emb1507 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	emb1507
Entrez Gene ID	838690
Full Name	U5 small nuclear ribonucleoprotein helicase
Synonyms	F9H16.5,F9H16_5,embryo defective 1507
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001198121.1	NP_001185050.1	U5 small nuclear ribonucleoprotein helicase
NM_101949.5	NP_173520.1	U5 small nuclear ribonucleoprotein helicase

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	brr2	NP_593346.1
Canis lupus familiaris (dog)	SNRNP200	XP_532949.2
Bos taurus (cattle)	SNRNP200	NP_001193092.1
Caenorhabditis elegans (roundworm)	snrp-200	NP_496710.1
Kluyveromyces lactis	KLLA0C17798g	XP_452995.1
Arabidopsis thaliana (thale cress)	AT2G42270	NP_181756.1
Pan troglodytes (chimpanzee)	SNRNP200	XP_001147672.1
Saccharomyces cerevisiae (baker's yeast)	BRR2	NP_011099.1
Eremothecium gossypii	AGOS_AGR113W	NP_986779.1
Neurospora crassa	NCU02685	XP_965458.1
Xenopus tropicalis (tropical clawed frog)	snrnp200	XP_002932581.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001519	XP_321605.3
Magnaporthe oryzae (rice blast fungus)	MGG_15536	XP_003713506.1
Homo sapiens (human)	SNRNP200	NP_054733.2
	SNRNP200	XP_001098299.2
Mus musculus (house mouse)	Snrnp200	NP_796188.2
Rattus norvegicus (Norway rat)	Snrnp200	NP_001032855.2
Danio rerio (zebrafish)	snrnp200	NP_001116729.1
Drosophila melanogaster (fruit fly)	l(3)72Ab	NP_648818.3
Arabidopsis thaliana (thale cress)	emb1507	NP_001185050.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BRR2a is specifically needed for efficient splicing of a subset of introns characterized by a combination of factors including intron size, sequence and chromatin, and that FLC is most sensitive to splicing defects [BRR2a]
Title: BRR2a Affects Flowering Time via FLC Splicing.

The following emb1507 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the emb1507 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb24102	NM_101949.5 Latest version!	Arabidopsis thaliana U5 small nuclear ribonucleoprotein helicase (emb1507), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAb24102	NM_001198121.1 Latest version!	Arabidopsis thaliana U5 small nuclear ribonucleoprotein helicase (emb1507), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

emb1507 ( NM_001198121.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001185050.1 Arabidopsis thaliana U5 small nuclear ribonucleoprotein helicase (emb1507), mRNA.

Price & Availability↑

CloneID	OAb24102
Clone ID Related Accession (Same CDS sequence)	NM_001198121.1 , NM_101949.5
Accession Version	NM_001198121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	U5 small nuclear ribonucleoprotein helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGCGAACT TGGGAGGCGG TGCGGAAGCA CATGCCAGGT TCAAGCAGTA TGAGTACAGA 
GCCAATTCTA GTCTGGTGTT AACTACTGAT AATAGACCTC GGGATACTCA TGAGCCTACA 
GGAGAACCGG AAACTCTGTG GGGGAAAATC GATCCGAGGT CTTTTGGTGA CCGTGTTGCT 
AAGGGAAGGC CTCAGGAGTT GGAGGATAAG CTGAAAAAGT CTAAGAAGAA GGAACGTGAT 
GTTGTAGATG ATATGGTAAA TATTAGGCAG AGTAAGAGGC GGCGTCTAAG GGAAGAGAGT 
GTGCTGACTG ATACTGATGA TGCGGTTTAT CAGCCGAAGA CTAAGGAGAC GAGGGCTGCT 
TATGAAGCCA TGCTTGGTCT TATCCAGAAG CAGTTGGGTG GGCAACCTCC GAGTATTGTT 
AGTGGTGCAG CGGATGAGAT TTTGGCTGTT CTCAAGAATG ACGCATTTAG AAACCCTGAG 
AAAAAGATGG AGATTGAGAA GTTGCTGAAC AAGATTGAAA ACCATGAGTT TGATCAGCTT 
GTTTCCATTG GAAAGTTAAT CACGGACTTC CAAGAGGGTG GTGATTCTGG TGGTGGTAGA 
GCTAATGATG ATGAAGGGCT TGATGATGAT TTGGGCGTGG CTGTTGAATT TGAGGAGAAT 
GAAGAAGATG ATGAGGAAAG CGATCCTGAT ATGGTTGAAG AGGATGATGA TGAGGAGGAT 
GATGAGCCTA CCAGAACTGG AGGCATGCAG GTTGATGCAG GGATAAATGA TGAGGATGCA 
GGAGATGCCA ATGAGGGCAC AAATCTTAAT GTTCAAGACA TTGATGCATA TTGGCTCCAG 
AGAAAGATCT CTCAAGCGTA TGAACAGCAG ATTGATCCAC AACAATGCCA GGTACTCGCA 
GAAGAGCTAC TAAAGATACT CGCTGAAGGG GATGATAGGG TTGTTGAGGA CAAGCTGCTT 
ATGCACCTTC AGTACGAAAA ATTTAGCCTT GTCAAGTTTC TGCTGCGTAA CCGTCTTAAA 
GTTGTGTGGT GCACTCGTTT GGCTAGGGCA GAAGACCAGG AAGAGAGGAA TAGAATCGAG 
GAGGAGATGA GGGGGTTAGG CCCAGAGTTG ACAGCAATTG TGGAGCAGTT GCATGCAACA 
AGAGCAACAG CGAAAGAGAG GGAGGAGAAC CTGCAAAAAA GTATTAACGA GGAAGCCCGG 
CGTTTGAAGG ACGAAACTGG TGGAGATGGA GGCAGAGGCA GGAGAGATGT TGCTGATAGA 
GATTCAGAGA GTGGGTGGGT GAAGGGTCAG CGTCAGATGC TGGACCTGGA GAGCTTGGCT 
TTTGACCAAG GTGGTCTTCT GATGGCGAAT AAGAAGTGTG ACCTCCCCCC TGGCTCTTAC 
AGAAGTCATG GCAAGGGATA TGATGAAGTT CATGTGCCTT GGGTTTCTAA AAAGGTTGAT 
AGGAATGAGA AGCTAGTGAA GATTACGGAA ATGCCTGATT GGGCGCAACC GGCTTTTAAG 
GGAATGCAAC AGTTGAACAG GGTTCAGAGC AAAGTGTATG ACACTGCGCT TTTCAAGGCA 
GAGAATATTC TTCTTTGTGC TCCAACTGGT GCTGGGAAAA CCAATGTTGC TATGCTCACC 
ATACTGCAGC AACTTGAAAT GAATAGGAAC ACAGATGGAA CTTATAACCA TGGAGATTAC 
AAAATTGTCT ATGTTGCACC CATGAAAGCC CTTGTTGCTG AAGTTGTAGG TAATTTGTCT 
AATCGTCTGA AGGATTATGG AGTTATTGTG AGAGAACTCA GTGGGGATCA GTCCCTAACC 
GGAAGAGAAA TTGAAGAGAC TCAGATAATT GTTACAACAC CAGAGAAATG GGATATCATC 
ACTAGGAAGT CTGGAGACCG AACTTATACT CAGCTTGTGA GACTTCTTAT AATTGATGAA 
ATTCATCTCC TTCATGACAA TCGAGGGCCT GTGCTTGAAA GCATTGTTGC TAGGACTCTT 
AGGCAAATTG AAACCACGAA AGAGAATATT CGTCTGGTTG GTTTATCTGC TACATTGCCA 
AATTATGAAG ACGTGGCCTT ATTTTTGCGG GTTGATCTTA AGAAAGGTTT GTTTAAATTT 
GACCGCAGCT ACAGACCCGT GCCTCTCCAT CAGCAGTATA TTGGAATCAG TGTGAAGAAA 
CCGTTGCAGA GGTTCCAGTT GATGAATGAT CTTTGTTATC AGAAAGTATT GGCTGGTGCT 
GGAAAGCACC AGGTCCTTAT TTTTGTTCAT TCAAGGAAGG AAACATCAAA AACTGCTAGA 
GCAATAAGGG ACACTGCAAT GGCGAACGAT ACCCTCAGCA GATTCTTGAA GGAAGATAGT 
GTAACTCGTG ACGTTCTCCA TAGTCATGAA GACATTGTTA AGAATAGTGA CCTAAAAGAT 
ATTCTGCCTT ACGGTTTTGC AATTCATCAT GCTGGGTTAT CAAGGGGTGA TCGTGAGATT 
GTTGAGACTC TTTTTTCTCA GGGGCATGTG CAAGTCTTGG TTTCCACAGC AACTCTCGCA 
TGGGGTGTGA ATCTGCCTGC ACATACCGTA ATTATAAAAG GAACCCAGGT GTATAATCCT 
GAAAAAGGGG CGTGGATGGA GCTGAGTCCT CTAGATGTTA TGCAGATGCT TGGTCGTGCT 
GGAAGACCTC AGTATGATCA ACATGGGGAG GGTATAATTA TAACTGGCTA CAGCGAGCTG 
CAATATTATC TTTCTTTGAT GAATGAGCAA CTACCTATTG AAAGCCAGTT TATTTCCAAA 
CTTGCTGATC AGCTTAATGC TGAAATTGTG CTTGGAACCG TTCAGAATGC TAGAGAGGCT 
TGCCATTGGC TTGGTTACAC GTATTTGTAT ATCCGTATGG TTCGTAATCC AACGTTGTAT 
GGCTTAGCGC CTGATGCCCT TGCAAAAGAC GTAGTTTTGG AGGAGAGAAG AGCTGACCTG 
ATACATTCGG CTGCTACTAT CTTGGACAAA AACAACTTGG TTAAGTATGA CAGGAAAAGT 
GGATACTTCC AAGTTACAGA TTTGGGACGC ATAGCAAGCT ACTATTACAT AACCCATGGG 
ACCATAGCTA CATACAATGA GCATTTGAAG CCAACAATGG GTGATATAGA TCTTTATCGT 
CTGTTCTCCC TGAGTGACGA GTTCAAGTAT GTGACTGTTC GGCAAGATGA GAAGATGGAA 
CTGGCGAAAC TTTTGGATCG TGTCCCAATT CCGATCAAGG AAACTTTGGA GGAGCCCAGT 
GCCAAAATAA ATGTATTGCT GCAGGCATAT ATTTCACAGC TAAAGCTTGA GGGTCTTTCT 
TTGACATCTG ACATGGTTTA CATTACGCAG AGTGCTGGAC GTCTTGTGCG AGCTCTTTAT 
GAAATTGTAT TGAAGCGGGG ATGGGCTCAA TTGGCAGAGA AGGCTCTGAA CTTGTCTAAA 
ATGGTAGGGA AGAGGATGTG GAGTGTTCAG ACACCCCTGC GTCAGTTTCA TGGACTCTCC 
AATGATATTT TGATGCAGTT GGAGAAGAAG GATTTGGTGT GGGAGAGGTA CTATGATCTA 
TCCGCACAGG AGCTAGGAGA GCTTATTCGC AGTCCTAAGA TGGGCAAGCC GCTTCACAAG 
TTCATTCACC AGTTCCCAAA AGTGACTCTT TCTGCACATG TTCAGCCTAT TACTCGCACT 
GTTCTGAATG TGGAGCTTAC AGTTACACCT GATTTCCTGT GGGACGAGAA AATTCATAAA 
TATGTTGAGC CGTTTTGGAT AATTGTGGAA GACAATGATG GTGAAAAGAT CCTTCATCAC 
GAGTATTTTC TTTTGAAGAA GCAGTATATT GATGAGGATC ACACTTTGCA CTTCACTGTG 
CCAATTTTTG AACCGTTGCC ACCTCAGTAT TTTGTTCGAG TGGTTTCAGA CAAGTGGCTT 
GGCTCGGAGA CTGTGTTGCC TGTATCTTTC AGGCATCTTA TTCTTCCAGA GAAGTATCCA 
CCTCCGACAG AGTTACTGGA CTTGCAACCT CTCCCAGTGA CGGCGCTAAG GAATCCGAAT 
TATGAGATAC TCTATCAGGA TTTCAAGCAT TTCAATCCAG TGCAGACTCA GGTCTTTACT 
GTTTTGTATA ACACTAACGA CAATGTGTTG GTTGCTGCTC CAACGGGAAG TGGGAAAACT 
ATTTGTGCGG AGTTCGCTAT TTTGAGGAAT CATCATGAAG GACCTGATGC TACAATGCGT 
GTTGTTTATA TTGCACCACT TGAGGCTATT GCCAAGGAGC AGTTTCGTAT CTGGGAGGGG 
AAGTTTGGGA AGGGTCTTGG TTTACGGGTT GTTGAGCTAA CTGGGGAGAC AGCATTGGAT 
CTTAAGCTGC TTGAGAAAGG CCAGATAATC ATAAGTACTC CTGAGAAATG GGATGCTCTT 
TCCCGCAGGT GGAAACAGAG AAAATATGTT CAACAAGTTA GTCTTTTCAT CGTCGACGAA 
CTCCATCTAA TTGGAGGTCA ACATGGTCCA GTGTTGGAGG TAATCGTTTC CAGAATGAGA 
TATATTTCAA GTCAGGTCAT TAATAAGATC AGGATTGTTG CGCTATCCAC TTCGCTTGCA 
AATGCAAAAG ATCTTGGGGA GTGGATTGGG GCCAGTTCCC ACGGCCTTTT CAACTTCCCT 
CCCGGAGTCC GTCCTGTTCC ACTGGAGATC CACATTCAGG GAGTGGATAT ATCCAGTTTT 
GAAGCCAGGA TGCAGGCGAT GACCAAACCA ACCTACACCG CCATAGTTCA GCACGCCAAG 
AACAAGAAGC CGGCTATTGT TTTCGTCCCA ACCCGTAAAC ATGTCCGCTT GACTGCTGTG 
GATCTGATGG CTTATTCTCA CATGGATAAC CCGCAGAGTC CTGATTTCTT GTTAGGGAAG 
CTGGAAGAAC TGGACCCTTT TGTTGAACAA ATCCGCGAGG AAACACTGAA GGAGACACTG 
TGTCATGGTA TTGGATACTT GCATGAGGGT TTAAGCAGTC TGGATCAGGA GATTGTGACA 
CAGCTCTTTG AAGCTGGACG GATTCAGGTT TGTGTGATGA GCAGTTCATT GTGTTGGGGC 
ACTCCATTAA CTGCGCATTT GGTGGTCGTA ATGGGAACGC AGTATTACGA TGGGAGGGAA 
AATTCACATT CTGATTATCC AGTCCCTGAT CTGCTTCAGA TGATGGGGCG TGCTAGCCGA 
CCACTTCTTG ACAATGCCGG GAAGTGTGTG ATCTTTTGTC ATGCACCGCG GAAGGAGTAT 
TACAAGAAGT TTTTGTACGA AGCCTTCCCT GTGGAAAGTC AGCTCCAGCA TTTCTTGCAT 
GATAATTTCA ACGCAGAAGT GGTTGCTGGG GTTATAGAGA ACAAGCAAGA TGCCGTGGAT 
TATCTTACAT GGACTTTCAT GTACAGGAGG CTTCCCCAGA ACCCTAACTA CTACAATCTC 
CAAGGTGTTA GCCACAGGCA TTTGTCTGAT CACCTATCAG AGCTGGTTGA GAACACCTTG 
TCTGATCTTG AAGCCAGTAA ATGCATTGAG GTAGAGGATG AGATGGAACT CTCTCCTCTC 
AACCTTGGTA TGATTGCATC CTATTATTAC ATCAGTTATA CCACCATTGA GCGCTTCAGT 
TCACTGTTGT CTTCTAAAAC CAAGATGAAG GGACTTCTTG AAATCTTAAC ATCGGCTTCA 
GAGTATGACA TGATTCCTAT ACGGCCTGGC GAAGAGGACA CCGTTCGAAG ATTAATCAAT 
CACCAGAGGT TCTCTTTTGA AAACCCAAAA TGCACAGACC CTCATGTGAA GGCAAACGCG 
CTTCTTCAGG CGCATTTCTC AAGGCAAAAC ATTGGCGGGA ACTTGGCAAT GGATCAGCGT 
GATGTCCTTC TATCTGCAAC CAGACTTCTT CAAGCGATGG TCGATGTGAT ATCAAGCAAT 
GGCTGGCTGA ATCTTGCTCT GCTAGCTATG GAAGTAAGCC AGATGGTGAC TCAAGGCATG 
TGGGAGCGGG ACTCCATGCT TCTGCAGCTC CCTCACTTCA CAAAAGACTT GGCCAAGAGG 
TGCCAGGAGA ATCCAGGGAA AAACATTGAA ACCGTATTCG ATCTTGTGGA GATGGAGGAT 
GAAGAACGCC AAGAGCTTCT CAAGATGTCA GACGCTCAGC TTCTAGACAT TGCCAGATTC 
TGCAACCGCT TCCCAAACAT CGACCTTACT TACGAGATTG TGGGCAGTGA AGAGGTGAAC 
CCAGGGAAAG AGGTTACATT GCAAGTGATG CTTGAGAGAG ACATGGAGGG GAGGACAGAA 
GTGGGACCTG TGGATTCACT GAGGTATCCC AAGACCAAAG AAGAAGGATG GTGGCTGGTC 
GTGGGCGACA CCAAGACAAA CCAGTTGCTG GCAATCAAGC GTGTCTCATT GCAACGGAAA 
GTGAAGGTGA AGCTGGATTT CACAGCACCG AGTGAACCAG GAGAGAAGTC ATACACACTA 
TACTTCATGT GTGACTCTTA CTTGGGGTGT GACCAAGAGT ACTCGTTCTC TGTTGATGTC 
AAAGGATCCG GCGCTGGAGA TCGCATGGAA GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185050.1
CDS	182..6697
Translation	MANLGGGAEAHARFKQYEYRANSSLVLTTDNRPRDTHEPTGEPETLWGKIDPRSFGDRVAKGRPQELEDKLKKSKKKERDVVDDMVNIRQSKRRRLREESVLTDTDDAVYQPKTKETRAAYEAMLGLIQKQLGGQPPSIVSGAADEILAVLKNDAFRNPEKKMEIEKLLNKIENHEFDQLVSIGKLITDFQEGGDSGGGRANDDEGLDDDLGVAVEFEENEEDDEESDPDMVEEDDDEEDDEPTRTGGMQVDAGINDEDAGDANEGTNLNVQDIDAYWLQRKISQAYEQQIDPQQCQVLAEELLKILAEGDDRVVEDKLLMHLQYEKFSLVKFLLRNRLKVVWCTRLARAEDQEERNRIEEEMRGLGPELTAIVEQLHATRATAKEREENLQKSINEEARRLKDETGGDGGRGRRDVADRDSESGWVKGQRQMLDLESLAFDQGGLLMANKKCDLPPGSYRSHGKGYDEVHVPWVSKKVDRNEKLVKITEMPDWAQPAFKGMQQLNRVQSKVYDTALFKAENILLCAPTGAGKTNVAMLTILQQLEMNRNTDGTYNHGDYKIVYVAPMKALVAEVVGNLSNRLKDYGVIVRELSGDQSLTGREIEETQIIVTTPEKWDIITRKSGDRTYTQLVRLLIIDEIHLLHDNRGPVLESIVARTLRQIETTKENIRLVGLSATLPNYEDVALFLRVDLKKGLFKFDRSYRPVPLHQQYIGISVKKPLQRFQLMNDLCYQKVLAGAGKHQVLIFVHSRKETSKTARAIRDTAMANDTLSRFLKEDSVTRDVLHSHEDIVKNSDLKDILPYGFAIHHAGLSRGDREIVETLFSQGHVQVLVSTATLAWGVNLPAHTVIIKGTQVYNPEKGAWMELSPLDVMQMLGRAGRPQYDQHGEGIIITGYSELQYYLSLMNEQLPIESQFISKLADQLNAEIVLGTVQNAREACHWLGYTYLYIRMVRNPTLYGLAPDALAKDVVLEERRADLIHSAATILDKNNLVKYDRKSGYFQVTDLGRIASYYYITHGTIATYNEHLKPTMGDIDLYRLFSLSDEFKYVTVRQDEKMELAKLLDRVPIPIKETLEEPSAKINVLLQAYISQLKLEGLSLTSDMVYITQSAGRLVRALYEIVLKRGWAQLAEKALNLSKMVGKRMWSVQTPLRQFHGLSNDILMQLEKKDLVWERYYDLSAQELGELIRSPKMGKPLHKFIHQFPKVTLSAHVQPITRTVLNVELTVTPDFLWDEKIHKYVEPFWIIVEDNDGEKILHHEYFLLKKQYIDEDHTLHFTVPIFEPLPPQYFVRVVSDKWLGSETVLPVSFRHLILPEKYPPPTELLDLQPLPVTALRNPNYEILYQDFKHFNPVQTQVFTVLYNTNDNVLVAAPTGSGKTICAEFAILRNHHEGPDATMRVVYIAPLEAIAKEQFRIWEGKFGKGLGLRVVELTGETALDLKLLEKGQIIISTPEKWDALSRRWKQRKYVQQVSLFIVDELHLIGGQHGPVLEVIVSRMRYISSQVINKIRIVALSTSLANAKDLGEWIGASSHGLFNFPPGVRPVPLEIHIQGVDISSFEARMQAMTKPTYTAIVQHAKNKKPAIVFVPTRKHVRLTAVDLMAYSHMDNPQSPDFLLGKLEELDPFVEQIREETLKETLCHGIGYLHEGLSSLDQEIVTQLFEAGRIQVCVMSSSLCWGTPLTAHLVVVMGTQYYDGRENSHSDYPVPDLLQMMGRASRPLLDNAGKCVIFCHAPRKEYYKKFLYEAFPVESQLQHFLHDNFNAEVVAGVIENKQDAVDYLTWTFMYRRLPQNPNYYNLQGVSHRHLSDHLSELVENTLSDLEASKCIEVEDEMELSPLNLGMIASYYYISYTTIERFSSLLSSKTKMKGLLEILTSASEYDMIPIRPGEEDTVRRLINHQRFSFENPKCTDPHVKANALLQAHFSRQNIGGNLAMDQRDVLLSATRLLQAMVDVISSNGWLNLALLAMEVSQMVTQGMWERDSMLLQLPHFTKDLAKRCQENPGKNIETVFDLVEMEDEERQELLKMSDAQLLDIARFCNRFPNIDLTYEIVGSEEVNPGKEVTLQVMLERDMEGRTEVGPVDSLRYPKTKEEGWWLVVGDTKTNQLLAIKRVSLQRKVKVKLDFTAPSEPGEKSYTLYFMCDSYLGCDQEYSFSVDVKGSGAGDRMEE

Target ORF information:

RefSeq Version	NM_001198121.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana U5 small nuclear ribonucleoprotein helicase (emb1507), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198121.1

ORF Insert Sequence:

ATGGCGAACT TGGGAGGCGG TGCGGAAGCA CATGCCAGGT TCAAGCAGTA TGAGTACAGA 
GCCAATTCTA GTCTGGTGTT AACTACTGAT AATAGACCTC GGGATACTCA TGAGCCTACA 
GGAGAACCGG AAACTCTGTG GGGGAAAATC GATCCGAGGT CTTTTGGTGA CCGTGTTGCT 
AAGGGAAGGC CTCAGGAGTT GGAGGATAAG CTGAAAAAGT CTAAGAAGAA GGAACGTGAT 
GTTGTAGATG ATATGGTAAA TATTAGGCAG AGTAAGAGGC GGCGTCTAAG GGAAGAGAGT 
GTGCTGACTG ATACTGATGA TGCGGTTTAT CAGCCGAAGA CTAAGGAGAC GAGGGCTGCT 
TATGAAGCCA TGCTTGGTCT TATCCAGAAG CAGTTGGGTG GGCAACCTCC GAGTATTGTT 
AGTGGTGCAG CGGATGAGAT TTTGGCTGTT CTCAAGAATG ACGCATTTAG AAACCCTGAG 
AAAAAGATGG AGATTGAGAA GTTGCTGAAC AAGATTGAAA ACCATGAGTT TGATCAGCTT 
GTTTCCATTG GAAAGTTAAT CACGGACTTC CAAGAGGGTG GTGATTCTGG TGGTGGTAGA 
GCTAATGATG ATGAAGGGCT TGATGATGAT TTGGGCGTGG CTGTTGAATT TGAGGAGAAT 
GAAGAAGATG ATGAGGAAAG CGATCCTGAT ATGGTTGAAG AGGATGATGA TGAGGAGGAT 
GATGAGCCTA CCAGAACTGG AGGCATGCAG GTTGATGCAG GGATAAATGA TGAGGATGCA 
GGAGATGCCA ATGAGGGCAC AAATCTTAAT GTTCAAGACA TTGATGCATA TTGGCTCCAG 
AGAAAGATCT CTCAAGCGTA TGAACAGCAG ATTGATCCAC AACAATGCCA GGTACTCGCA 
GAAGAGCTAC TAAAGATACT CGCTGAAGGG GATGATAGGG TTGTTGAGGA CAAGCTGCTT 
ATGCACCTTC AGTACGAAAA ATTTAGCCTT GTCAAGTTTC TGCTGCGTAA CCGTCTTAAA 
GTTGTGTGGT GCACTCGTTT GGCTAGGGCA GAAGACCAGG AAGAGAGGAA TAGAATCGAG 
GAGGAGATGA GGGGGTTAGG CCCAGAGTTG ACAGCAATTG TGGAGCAGTT GCATGCAACA 
AGAGCAACAG CGAAAGAGAG GGAGGAGAAC CTGCAAAAAA GTATTAACGA GGAAGCCCGG 
CGTTTGAAGG ACGAAACTGG TGGAGATGGA GGCAGAGGCA GGAGAGATGT TGCTGATAGA 
GATTCAGAGA GTGGGTGGGT GAAGGGTCAG CGTCAGATGC TGGACCTGGA GAGCTTGGCT 
TTTGACCAAG GTGGTCTTCT GATGGCGAAT AAGAAGTGTG ACCTCCCCCC TGGCTCTTAC 
AGAAGTCATG GCAAGGGATA TGATGAAGTT CATGTGCCTT GGGTTTCTAA AAAGGTTGAT 
AGGAATGAGA AGCTAGTGAA GATTACGGAA ATGCCTGATT GGGCGCAACC GGCTTTTAAG 
GGAATGCAAC AGTTGAACAG GGTTCAGAGC AAAGTGTATG ACACTGCGCT TTTCAAGGCA 
GAGAATATTC TTCTTTGTGC TCCAACTGGT GCTGGGAAAA CCAATGTTGC TATGCTCACC 
ATACTGCAGC AACTTGAAAT GAATAGGAAC ACAGATGGAA CTTATAACCA TGGAGATTAC 
AAAATTGTCT ATGTTGCACC CATGAAAGCC CTTGTTGCTG AAGTTGTAGG TAATTTGTCT 
AATCGTCTGA AGGATTATGG AGTTATTGTG AGAGAACTCA GTGGGGATCA GTCCCTAACC 
GGAAGAGAAA TTGAAGAGAC TCAGATAATT GTTACAACAC CAGAGAAATG GGATATCATC 
ACTAGGAAGT CTGGAGACCG AACTTATACT CAGCTTGTGA GACTTCTTAT AATTGATGAA 
ATTCATCTCC TTCATGACAA TCGAGGGCCT GTGCTTGAAA GCATTGTTGC TAGGACTCTT 
AGGCAAATTG AAACCACGAA AGAGAATATT CGTCTGGTTG GTTTATCTGC TACATTGCCA 
AATTATGAAG ACGTGGCCTT ATTTTTGCGG GTTGATCTTA AGAAAGGTTT GTTTAAATTT 
GACCGCAGCT ACAGACCCGT GCCTCTCCAT CAGCAGTATA TTGGAATCAG TGTGAAGAAA 
CCGTTGCAGA GGTTCCAGTT GATGAATGAT CTTTGTTATC AGAAAGTATT GGCTGGTGCT 
GGAAAGCACC AGGTCCTTAT TTTTGTTCAT TCAAGGAAGG AAACATCAAA AACTGCTAGA 
GCAATAAGGG ACACTGCAAT GGCGAACGAT ACCCTCAGCA GATTCTTGAA GGAAGATAGT 
GTAACTCGTG ACGTTCTCCA TAGTCATGAA GACATTGTTA AGAATAGTGA CCTAAAAGAT 
ATTCTGCCTT ACGGTTTTGC AATTCATCAT GCTGGGTTAT CAAGGGGTGA TCGTGAGATT 
GTTGAGACTC TTTTTTCTCA GGGGCATGTG CAAGTCTTGG TTTCCACAGC AACTCTCGCA 
TGGGGTGTGA ATCTGCCTGC ACATACCGTA ATTATAAAAG GAACCCAGGT GTATAATCCT 
GAAAAAGGGG CGTGGATGGA GCTGAGTCCT CTAGATGTTA TGCAGATGCT TGGTCGTGCT 
GGAAGACCTC AGTATGATCA ACATGGGGAG GGTATAATTA TAACTGGCTA CAGCGAGCTG 
CAATATTATC TTTCTTTGAT GAATGAGCAA CTACCTATTG AAAGCCAGTT TATTTCCAAA 
CTTGCTGATC AGCTTAATGC TGAAATTGTG CTTGGAACCG TTCAGAATGC TAGAGAGGCT 
TGCCATTGGC TTGGTTACAC GTATTTGTAT ATCCGTATGG TTCGTAATCC AACGTTGTAT 
GGCTTAGCGC CTGATGCCCT TGCAAAAGAC GTAGTTTTGG AGGAGAGAAG AGCTGACCTG 
ATACATTCGG CTGCTACTAT CTTGGACAAA AACAACTTGG TTAAGTATGA CAGGAAAAGT 
GGATACTTCC AAGTTACAGA TTTGGGACGC ATAGCAAGCT ACTATTACAT AACCCATGGG 
ACCATAGCTA CATACAATGA GCATTTGAAG CCAACAATGG GTGATATAGA TCTTTATCGT 
CTGTTCTCCC TGAGTGACGA GTTCAAGTAT GTGACTGTTC GGCAAGATGA GAAGATGGAA 
CTGGCGAAAC TTTTGGATCG TGTCCCAATT CCGATCAAGG AAACTTTGGA GGAGCCCAGT 
GCCAAAATAA ATGTATTGCT GCAGGCATAT ATTTCACAGC TAAAGCTTGA GGGTCTTTCT 
TTGACATCTG ACATGGTTTA CATTACGCAG AGTGCTGGAC GTCTTGTGCG AGCTCTTTAT 
GAAATTGTAT TGAAGCGGGG ATGGGCTCAA TTGGCAGAGA AGGCTCTGAA CTTGTCTAAA 
ATGGTAGGGA AGAGGATGTG GAGTGTTCAG ACACCCCTGC GTCAGTTTCA TGGACTCTCC 
AATGATATTT TGATGCAGTT GGAGAAGAAG GATTTGGTGT GGGAGAGGTA CTATGATCTA 
TCCGCACAGG AGCTAGGAGA GCTTATTCGC AGTCCTAAGA TGGGCAAGCC GCTTCACAAG 
TTCATTCACC AGTTCCCAAA AGTGACTCTT TCTGCACATG TTCAGCCTAT TACTCGCACT 
GTTCTGAATG TGGAGCTTAC AGTTACACCT GATTTCCTGT GGGACGAGAA AATTCATAAA 
TATGTTGAGC CGTTTTGGAT AATTGTGGAA GACAATGATG GTGAAAAGAT CCTTCATCAC 
GAGTATTTTC TTTTGAAGAA GCAGTATATT GATGAGGATC ACACTTTGCA CTTCACTGTG 
CCAATTTTTG AACCGTTGCC ACCTCAGTAT TTTGTTCGAG TGGTTTCAGA CAAGTGGCTT 
GGCTCGGAGA CTGTGTTGCC TGTATCTTTC AGGCATCTTA TTCTTCCAGA GAAGTATCCA 
CCTCCGACAG AGTTACTGGA CTTGCAACCT CTCCCAGTGA CGGCGCTAAG GAATCCGAAT 
TATGAGATAC TCTATCAGGA TTTCAAGCAT TTCAATCCAG TGCAGACTCA GGTCTTTACT 
GTTTTGTATA ACACTAACGA CAATGTGTTG GTTGCTGCTC CAACGGGAAG TGGGAAAACT 
ATTTGTGCGG AGTTCGCTAT TTTGAGGAAT CATCATGAAG GACCTGATGC TACAATGCGT 
GTTGTTTATA TTGCACCACT TGAGGCTATT GCCAAGGAGC AGTTTCGTAT CTGGGAGGGG 
AAGTTTGGGA AGGGTCTTGG TTTACGGGTT GTTGAGCTAA CTGGGGAGAC AGCATTGGAT 
CTTAAGCTGC TTGAGAAAGG CCAGATAATC ATAAGTACTC CTGAGAAATG GGATGCTCTT 
TCCCGCAGGT GGAAACAGAG AAAATATGTT CAACAAGTTA GTCTTTTCAT CGTCGACGAA 
CTCCATCTAA TTGGAGGTCA ACATGGTCCA GTGTTGGAGG TAATCGTTTC CAGAATGAGA 
TATATTTCAA GTCAGGTCAT TAATAAGATC AGGATTGTTG CGCTATCCAC TTCGCTTGCA 
AATGCAAAAG ATCTTGGGGA GTGGATTGGG GCCAGTTCCC ACGGCCTTTT CAACTTCCCT 
CCCGGAGTCC GTCCTGTTCC ACTGGAGATC CACATTCAGG GAGTGGATAT ATCCAGTTTT 
GAAGCCAGGA TGCAGGCGAT GACCAAACCA ACCTACACCG CCATAGTTCA GCACGCCAAG 
AACAAGAAGC CGGCTATTGT TTTCGTCCCA ACCCGTAAAC ATGTCCGCTT GACTGCTGTG 
GATCTGATGG CTTATTCTCA CATGGATAAC CCGCAGAGTC CTGATTTCTT GTTAGGGAAG 
CTGGAAGAAC TGGACCCTTT TGTTGAACAA ATCCGCGAGG AAACACTGAA GGAGACACTG 
TGTCATGGTA TTGGATACTT GCATGAGGGT TTAAGCAGTC TGGATCAGGA GATTGTGACA 
CAGCTCTTTG AAGCTGGACG GATTCAGGTT TGTGTGATGA GCAGTTCATT GTGTTGGGGC 
ACTCCATTAA CTGCGCATTT GGTGGTCGTA ATGGGAACGC AGTATTACGA TGGGAGGGAA 
AATTCACATT CTGATTATCC AGTCCCTGAT CTGCTTCAGA TGATGGGGCG TGCTAGCCGA 
CCACTTCTTG ACAATGCCGG GAAGTGTGTG ATCTTTTGTC ATGCACCGCG GAAGGAGTAT 
TACAAGAAGT TTTTGTACGA AGCCTTCCCT GTGGAAAGTC AGCTCCAGCA TTTCTTGCAT 
GATAATTTCA ACGCAGAAGT GGTTGCTGGG GTTATAGAGA ACAAGCAAGA TGCCGTGGAT 
TATCTTACAT GGACTTTCAT GTACAGGAGG CTTCCCCAGA ACCCTAACTA CTACAATCTC 
CAAGGTGTTA GCCACAGGCA TTTGTCTGAT CACCTATCAG AGCTGGTTGA GAACACCTTG 
TCTGATCTTG AAGCCAGTAA ATGCATTGAG GTAGAGGATG AGATGGAACT CTCTCCTCTC 
AACCTTGGTA TGATTGCATC CTATTATTAC ATCAGTTATA CCACCATTGA GCGCTTCAGT 
TCACTGTTGT CTTCTAAAAC CAAGATGAAG GGACTTCTTG AAATCTTAAC ATCGGCTTCA 
GAGTATGACA TGATTCCTAT ACGGCCTGGC GAAGAGGACA CCGTTCGAAG ATTAATCAAT 
CACCAGAGGT TCTCTTTTGA AAACCCAAAA TGCACAGACC CTCATGTGAA GGCAAACGCG 
CTTCTTCAGG CGCATTTCTC AAGGCAAAAC ATTGGCGGGA ACTTGGCAAT GGATCAGCGT 
GATGTCCTTC TATCTGCAAC CAGACTTCTT CAAGCGATGG TCGATGTGAT ATCAAGCAAT 
GGCTGGCTGA ATCTTGCTCT GCTAGCTATG GAAGTAAGCC AGATGGTGAC TCAAGGCATG 
TGGGAGCGGG ACTCCATGCT TCTGCAGCTC CCTCACTTCA CAAAAGACTT GGCCAAGAGG 
TGCCAGGAGA ATCCAGGGAA AAACATTGAA ACCGTATTCG ATCTTGTGGA GATGGAGGAT 
GAAGAACGCC AAGAGCTTCT CAAGATGTCA GACGCTCAGC TTCTAGACAT TGCCAGATTC 
TGCAACCGCT TCCCAAACAT CGACCTTACT TACGAGATTG TGGGCAGTGA AGAGGTGAAC 
CCAGGGAAAG AGGTTACATT GCAAGTGATG CTTGAGAGAG ACATGGAGGG GAGGACAGAA 
GTGGGACCTG TGGATTCACT GAGGTATCCC AAGACCAAAG AAGAAGGATG GTGGCTGGTC 
GTGGGCGACA CCAAGACAAA CCAGTTGCTG GCAATCAAGC GTGTCTCATT GCAACGGAAA 
GTGAAGGTGA AGCTGGATTT CACAGCACCG AGTGAACCAG GAGAGAAGTC ATACACACTA 
TACTTCATGT GTGACTCTTA CTTGGGGTGT GACCAAGAGT ACTCGTTCTC TGTTGATGTC 
AAAGGATCCG GCGCTGGAGA TCGCATGGAA GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emb1507 ( NM_101949.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_173520.1 Arabidopsis thaliana U5 small nuclear ribonucleoprotein helicase (emb1507), mRNA.

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CloneID	OAb24102
Clone ID Related Accession (Same CDS sequence)	NM_001198121.1 , NM_101949.5
Accession Version	NM_101949.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	U5 small nuclear ribonucleoprotein helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_101949.4.

ATGGCGAACT TGGGAGGCGG TGCGGAAGCA CATGCCAGGT TCAAGCAGTA TGAGTACAGA 
GCCAATTCTA GTCTGGTGTT AACTACTGAT AATAGACCTC GGGATACTCA TGAGCCTACA 
GGAGAACCGG AAACTCTGTG GGGGAAAATC GATCCGAGGT CTTTTGGTGA CCGTGTTGCT 
AAGGGAAGGC CTCAGGAGTT GGAGGATAAG CTGAAAAAGT CTAAGAAGAA GGAACGTGAT 
GTTGTAGATG ATATGGTAAA TATTAGGCAG AGTAAGAGGC GGCGTCTAAG GGAAGAGAGT 
GTGCTGACTG ATACTGATGA TGCGGTTTAT CAGCCGAAGA CTAAGGAGAC GAGGGCTGCT 
TATGAAGCCA TGCTTGGTCT TATCCAGAAG CAGTTGGGTG GGCAACCTCC GAGTATTGTT 
AGTGGTGCAG CGGATGAGAT TTTGGCTGTT CTCAAGAATG ACGCATTTAG AAACCCTGAG 
AAAAAGATGG AGATTGAGAA GTTGCTGAAC AAGATTGAAA ACCATGAGTT TGATCAGCTT 
GTTTCCATTG GAAAGTTAAT CACGGACTTC CAAGAGGGTG GTGATTCTGG TGGTGGTAGA 
GCTAATGATG ATGAAGGGCT TGATGATGAT TTGGGCGTGG CTGTTGAATT TGAGGAGAAT 
GAAGAAGATG ATGAGGAAAG CGATCCTGAT ATGGTTGAAG AGGATGATGA TGAGGAGGAT 
GATGAGCCTA CCAGAACTGG AGGCATGCAG GTTGATGCAG GGATAAATGA TGAGGATGCA 
GGAGATGCCA ATGAGGGCAC AAATCTTAAT GTTCAAGACA TTGATGCATA TTGGCTCCAG 
AGAAAGATCT CTCAAGCGTA TGAACAGCAG ATTGATCCAC AACAATGCCA GGTACTCGCA 
GAAGAGCTAC TAAAGATACT CGCTGAAGGG GATGATAGGG TTGTTGAGGA CAAGCTGCTT 
ATGCACCTTC AGTACGAAAA ATTTAGCCTT GTCAAGTTTC TGCTGCGTAA CCGTCTTAAA 
GTTGTGTGGT GCACTCGTTT GGCTAGGGCA GAAGACCAGG AAGAGAGGAA TAGAATCGAG 
GAGGAGATGA GGGGGTTAGG CCCAGAGTTG ACAGCAATTG TGGAGCAGTT GCATGCAACA 
AGAGCAACAG CGAAAGAGAG GGAGGAGAAC CTGCAAAAAA GTATTAACGA GGAAGCCCGG 
CGTTTGAAGG ACGAAACTGG TGGAGATGGA GGCAGAGGCA GGAGAGATGT TGCTGATAGA 
GATTCAGAGA GTGGGTGGGT GAAGGGTCAG CGTCAGATGC TGGACCTGGA GAGCTTGGCT 
TTTGACCAAG GTGGTCTTCT GATGGCGAAT AAGAAGTGTG ACCTCCCCCC TGGCTCTTAC 
AGAAGTCATG GCAAGGGATA TGATGAAGTT CATGTGCCTT GGGTTTCTAA AAAGGTTGAT 
AGGAATGAGA AGCTAGTGAA GATTACGGAA ATGCCTGATT GGGCGCAACC GGCTTTTAAG 
GGAATGCAAC AGTTGAACAG GGTTCAGAGC AAAGTGTATG ACACTGCGCT TTTCAAGGCA 
GAGAATATTC TTCTTTGTGC TCCAACTGGT GCTGGGAAAA CCAATGTTGC TATGCTCACC 
ATACTGCAGC AACTTGAAAT GAATAGGAAC ACAGATGGAA CTTATAACCA TGGAGATTAC 
AAAATTGTCT ATGTTGCACC CATGAAAGCC CTTGTTGCTG AAGTTGTAGG TAATTTGTCT 
AATCGTCTGA AGGATTATGG AGTTATTGTG AGAGAACTCA GTGGGGATCA GTCCCTAACC 
GGAAGAGAAA TTGAAGAGAC TCAGATAATT GTTACAACAC CAGAGAAATG GGATATCATC 
ACTAGGAAGT CTGGAGACCG AACTTATACT CAGCTTGTGA GACTTCTTAT AATTGATGAA 
ATTCATCTCC TTCATGACAA TCGAGGGCCT GTGCTTGAAA GCATTGTTGC TAGGACTCTT 
AGGCAAATTG AAACCACGAA AGAGAATATT CGTCTGGTTG GTTTATCTGC TACATTGCCA 
AATTATGAAG ACGTGGCCTT ATTTTTGCGG GTTGATCTTA AGAAAGGTTT GTTTAAATTT 
GACCGCAGCT ACAGACCCGT GCCTCTCCAT CAGCAGTATA TTGGAATCAG TGTGAAGAAA 
CCGTTGCAGA GGTTCCAGTT GATGAATGAT CTTTGTTATC AGAAAGTATT GGCTGGTGCT 
GGAAAGCACC AGGTCCTTAT TTTTGTTCAT TCAAGGAAGG AAACATCAAA AACTGCTAGA 
GCAATAAGGG ACACTGCAAT GGCGAACGAT ACCCTCAGCA GATTCTTGAA GGAAGATAGT 
GTAACTCGTG ACGTTCTCCA TAGTCATGAA GACATTGTTA AGAATAGTGA CCTAAAAGAT 
ATTCTGCCTT ACGGTTTTGC AATTCATCAT GCTGGGTTAT CAAGGGGTGA TCGTGAGATT 
GTTGAGACTC TTTTTTCTCA GGGGCATGTG CAAGTCTTGG TTTCCACAGC AACTCTCGCA 
TGGGGTGTGA ATCTGCCTGC ACATACCGTA ATTATAAAAG GAACCCAGGT GTATAATCCT 
GAAAAAGGGG CGTGGATGGA GCTGAGTCCT CTAGATGTTA TGCAGATGCT TGGTCGTGCT 
GGAAGACCTC AGTATGATCA ACATGGGGAG GGTATAATTA TAACTGGCTA CAGCGAGCTG 
CAATATTATC TTTCTTTGAT GAATGAGCAA CTACCTATTG AAAGCCAGTT TATTTCCAAA 
CTTGCTGATC AGCTTAATGC TGAAATTGTG CTTGGAACCG TTCAGAATGC TAGAGAGGCT 
TGCCATTGGC TTGGTTACAC GTATTTGTAT ATCCGTATGG TTCGTAATCC AACGTTGTAT 
GGCTTAGCGC CTGATGCCCT TGCAAAAGAC GTAGTTTTGG AGGAGAGAAG AGCTGACCTG 
ATACATTCGG CTGCTACTAT CTTGGACAAA AACAACTTGG TTAAGTATGA CAGGAAAAGT 
GGATACTTCC AAGTTACAGA TTTGGGACGC ATAGCAAGCT ACTATTACAT AACCCATGGG 
ACCATAGCTA CATACAATGA GCATTTGAAG CCAACAATGG GTGATATAGA TCTTTATCGT 
CTGTTCTCCC TGAGTGACGA GTTCAAGTAT GTGACTGTTC GGCAAGATGA GAAGATGGAA 
CTGGCGAAAC TTTTGGATCG TGTCCCAATT CCGATCAAGG AAACTTTGGA GGAGCCCAGT 
GCCAAAATAA ATGTATTGCT GCAGGCATAT ATTTCACAGC TAAAGCTTGA GGGTCTTTCT 
TTGACATCTG ACATGGTTTA CATTACGCAG AGTGCTGGAC GTCTTGTGCG AGCTCTTTAT 
GAAATTGTAT TGAAGCGGGG ATGGGCTCAA TTGGCAGAGA AGGCTCTGAA CTTGTCTAAA 
ATGGTAGGGA AGAGGATGTG GAGTGTTCAG ACACCCCTGC GTCAGTTTCA TGGACTCTCC 
AATGATATTT TGATGCAGTT GGAGAAGAAG GATTTGGTGT GGGAGAGGTA CTATGATCTA 
TCCGCACAGG AGCTAGGAGA GCTTATTCGC AGTCCTAAGA TGGGCAAGCC GCTTCACAAG 
TTCATTCACC AGTTCCCAAA AGTGACTCTT TCTGCACATG TTCAGCCTAT TACTCGCACT 
GTTCTGAATG TGGAGCTTAC AGTTACACCT GATTTCCTGT GGGACGAGAA AATTCATAAA 
TATGTTGAGC CGTTTTGGAT AATTGTGGAA GACAATGATG GTGAAAAGAT CCTTCATCAC 
GAGTATTTTC TTTTGAAGAA GCAGTATATT GATGAGGATC ACACTTTGCA CTTCACTGTG 
CCAATTTTTG AACCGTTGCC ACCTCAGTAT TTTGTTCGAG TGGTTTCAGA CAAGTGGCTT 
GGCTCGGAGA CTGTGTTGCC TGTATCTTTC AGGCATCTTA TTCTTCCAGA GAAGTATCCA 
CCTCCGACAG AGTTACTGGA CTTGCAACCT CTCCCAGTGA CGGCGCTAAG GAATCCGAAT 
TATGAGATAC TCTATCAGGA TTTCAAGCAT TTCAATCCAG TGCAGACTCA GGTCTTTACT 
GTTTTGTATA ACACTAACGA CAATGTGTTG GTTGCTGCTC CAACGGGAAG TGGGAAAACT 
ATTTGTGCGG AGTTCGCTAT TTTGAGGAAT CATCATGAAG GACCTGATGC TACAATGCGT 
GTTGTTTATA TTGCACCACT TGAGGCTATT GCCAAGGAGC AGTTTCGTAT CTGGGAGGGG 
AAGTTTGGGA AGGGTCTTGG TTTACGGGTT GTTGAGCTAA CTGGGGAGAC AGCATTGGAT 
CTTAAGCTGC TTGAGAAAGG CCAGATAATC ATAAGTACTC CTGAGAAATG GGATGCTCTT 
TCCCGCAGGT GGAAACAGAG AAAATATGTT CAACAAGTTA GTCTTTTCAT CGTCGACGAA 
CTCCATCTAA TTGGAGGTCA ACATGGTCCA GTGTTGGAGG TAATCGTTTC CAGAATGAGA 
TATATTTCAA GTCAGGTCAT TAATAAGATC AGGATTGTTG CGCTATCCAC TTCGCTTGCA 
AATGCAAAAG ATCTTGGGGA GTGGATTGGG GCCAGTTCCC ACGGCCTTTT CAACTTCCCT 
CCCGGAGTCC GTCCTGTTCC ACTGGAGATC CACATTCAGG GAGTGGATAT ATCCAGTTTT 
GAAGCCAGGA TGCAGGCGAT GACCAAACCA ACCTACACCG CCATAGTTCA GCACGCCAAG 
AACAAGAAGC CGGCTATTGT TTTCGTCCCA ACCCGTAAAC ATGTCCGCTT GACTGCTGTG 
GATCTGATGG CTTATTCTCA CATGGATAAC CCGCAGAGTC CTGATTTCTT GTTAGGGAAG 
CTGGAAGAAC TGGACCCTTT TGTTGAACAA ATCCGCGAGG AAACACTGAA GGAGACACTG 
TGTCATGGTA TTGGATACTT GCATGAGGGT TTAAGCAGTC TGGATCAGGA GATTGTGACA 
CAGCTCTTTG AAGCTGGACG GATTCAGGTT TGTGTGATGA GCAGTTCATT GTGTTGGGGC 
ACTCCATTAA CTGCGCATTT GGTGGTCGTA ATGGGAACGC AGTATTACGA TGGGAGGGAA 
AATTCACATT CTGATTATCC AGTCCCTGAT CTGCTTCAGA TGATGGGGCG TGCTAGCCGA 
CCACTTCTTG ACAATGCCGG GAAGTGTGTG ATCTTTTGTC ATGCACCGCG GAAGGAGTAT 
TACAAGAAGT TTTTGTACGA AGCCTTCCCT GTGGAAAGTC AGCTCCAGCA TTTCTTGCAT 
GATAATTTCA ACGCAGAAGT GGTTGCTGGG GTTATAGAGA ACAAGCAAGA TGCCGTGGAT 
TATCTTACAT GGACTTTCAT GTACAGGAGG CTTCCCCAGA ACCCTAACTA CTACAATCTC 
CAAGGTGTTA GCCACAGGCA TTTGTCTGAT CACCTATCAG AGCTGGTTGA GAACACCTTG 
TCTGATCTTG AAGCCAGTAA ATGCATTGAG GTAGAGGATG AGATGGAACT CTCTCCTCTC 
AACCTTGGTA TGATTGCATC CTATTATTAC ATCAGTTATA CCACCATTGA GCGCTTCAGT 
TCACTGTTGT CTTCTAAAAC CAAGATGAAG GGACTTCTTG AAATCTTAAC ATCGGCTTCA 
GAGTATGACA TGATTCCTAT ACGGCCTGGC GAAGAGGACA CCGTTCGAAG ATTAATCAAT 
CACCAGAGGT TCTCTTTTGA AAACCCAAAA TGCACAGACC CTCATGTGAA GGCAAACGCG 
CTTCTTCAGG CGCATTTCTC AAGGCAAAAC ATTGGCGGGA ACTTGGCAAT GGATCAGCGT 
GATGTCCTTC TATCTGCAAC CAGACTTCTT CAAGCGATGG TCGATGTGAT ATCAAGCAAT 
GGCTGGCTGA ATCTTGCTCT GCTAGCTATG GAAGTAAGCC AGATGGTGAC TCAAGGCATG 
TGGGAGCGGG ACTCCATGCT TCTGCAGCTC CCTCACTTCA CAAAAGACTT GGCCAAGAGG 
TGCCAGGAGA ATCCAGGGAA AAACATTGAA ACCGTATTCG ATCTTGTGGA GATGGAGGAT 
GAAGAACGCC AAGAGCTTCT CAAGATGTCA GACGCTCAGC TTCTAGACAT TGCCAGATTC 
TGCAACCGCT TCCCAAACAT CGACCTTACT TACGAGATTG TGGGCAGTGA AGAGGTGAAC 
CCAGGGAAAG AGGTTACATT GCAAGTGATG CTTGAGAGAG ACATGGAGGG GAGGACAGAA 
GTGGGACCTG TGGATTCACT GAGGTATCCC AAGACCAAAG AAGAAGGATG GTGGCTGGTC 
GTGGGCGACA CCAAGACAAA CCAGTTGCTG GCAATCAAGC GTGTCTCATT GCAACGGAAA 
GTGAAGGTGA AGCTGGATTT CACAGCACCG AGTGAACCAG GAGAGAAGTC ATACACACTA 
TACTTCATGT GTGACTCTTA CTTGGGGTGT GACCAAGAGT ACTCGTTCTC TGTTGATGTC 
AAAGGATCCG GCGCTGGAGA TCGCATGGAA GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_173520.1
CDS	203..6718
Translation	MANLGGGAEAHARFKQYEYRANSSLVLTTDNRPRDTHEPTGEPETLWGKIDPRSFGDRVAKGRPQELEDKLKKSKKKERDVVDDMVNIRQSKRRRLREESVLTDTDDAVYQPKTKETRAAYEAMLGLIQKQLGGQPPSIVSGAADEILAVLKNDAFRNPEKKMEIEKLLNKIENHEFDQLVSIGKLITDFQEGGDSGGGRANDDEGLDDDLGVAVEFEENEEDDEESDPDMVEEDDDEEDDEPTRTGGMQVDAGINDEDAGDANEGTNLNVQDIDAYWLQRKISQAYEQQIDPQQCQVLAEELLKILAEGDDRVVEDKLLMHLQYEKFSLVKFLLRNRLKVVWCTRLARAEDQEERNRIEEEMRGLGPELTAIVEQLHATRATAKEREENLQKSINEEARRLKDETGGDGGRGRRDVADRDSESGWVKGQRQMLDLESLAFDQGGLLMANKKCDLPPGSYRSHGKGYDEVHVPWVSKKVDRNEKLVKITEMPDWAQPAFKGMQQLNRVQSKVYDTALFKAENILLCAPTGAGKTNVAMLTILQQLEMNRNTDGTYNHGDYKIVYVAPMKALVAEVVGNLSNRLKDYGVIVRELSGDQSLTGREIEETQIIVTTPEKWDIITRKSGDRTYTQLVRLLIIDEIHLLHDNRGPVLESIVARTLRQIETTKENIRLVGLSATLPNYEDVALFLRVDLKKGLFKFDRSYRPVPLHQQYIGISVKKPLQRFQLMNDLCYQKVLAGAGKHQVLIFVHSRKETSKTARAIRDTAMANDTLSRFLKEDSVTRDVLHSHEDIVKNSDLKDILPYGFAIHHAGLSRGDREIVETLFSQGHVQVLVSTATLAWGVNLPAHTVIIKGTQVYNPEKGAWMELSPLDVMQMLGRAGRPQYDQHGEGIIITGYSELQYYLSLMNEQLPIESQFISKLADQLNAEIVLGTVQNAREACHWLGYTYLYIRMVRNPTLYGLAPDALAKDVVLEERRADLIHSAATILDKNNLVKYDRKSGYFQVTDLGRIASYYYITHGTIATYNEHLKPTMGDIDLYRLFSLSDEFKYVTVRQDEKMELAKLLDRVPIPIKETLEEPSAKINVLLQAYISQLKLEGLSLTSDMVYITQSAGRLVRALYEIVLKRGWAQLAEKALNLSKMVGKRMWSVQTPLRQFHGLSNDILMQLEKKDLVWERYYDLSAQELGELIRSPKMGKPLHKFIHQFPKVTLSAHVQPITRTVLNVELTVTPDFLWDEKIHKYVEPFWIIVEDNDGEKILHHEYFLLKKQYIDEDHTLHFTVPIFEPLPPQYFVRVVSDKWLGSETVLPVSFRHLILPEKYPPPTELLDLQPLPVTALRNPNYEILYQDFKHFNPVQTQVFTVLYNTNDNVLVAAPTGSGKTICAEFAILRNHHEGPDATMRVVYIAPLEAIAKEQFRIWEGKFGKGLGLRVVELTGETALDLKLLEKGQIIISTPEKWDALSRRWKQRKYVQQVSLFIVDELHLIGGQHGPVLEVIVSRMRYISSQVINKIRIVALSTSLANAKDLGEWIGASSHGLFNFPPGVRPVPLEIHIQGVDISSFEARMQAMTKPTYTAIVQHAKNKKPAIVFVPTRKHVRLTAVDLMAYSHMDNPQSPDFLLGKLEELDPFVEQIREETLKETLCHGIGYLHEGLSSLDQEIVTQLFEAGRIQVCVMSSSLCWGTPLTAHLVVVMGTQYYDGRENSHSDYPVPDLLQMMGRASRPLLDNAGKCVIFCHAPRKEYYKKFLYEAFPVESQLQHFLHDNFNAEVVAGVIENKQDAVDYLTWTFMYRRLPQNPNYYNLQGVSHRHLSDHLSELVENTLSDLEASKCIEVEDEMELSPLNLGMIASYYYISYTTIERFSSLLSSKTKMKGLLEILTSASEYDMIPIRPGEEDTVRRLINHQRFSFENPKCTDPHVKANALLQAHFSRQNIGGNLAMDQRDVLLSATRLLQAMVDVISSNGWLNLALLAMEVSQMVTQGMWERDSMLLQLPHFTKDLAKRCQENPGKNIETVFDLVEMEDEERQELLKMSDAQLLDIARFCNRFPNIDLTYEIVGSEEVNPGKEVTLQVMLERDMEGRTEVGPVDSLRYPKTKEEGWWLVVGDTKTNQLLAIKRVSLQRKVKVKLDFTAPSEPGEKSYTLYFMCDSYLGCDQEYSFSVDVKGSGAGDRMEE

Target ORF information:

RefSeq Version	NM_101949.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana U5 small nuclear ribonucleoprotein helicase (emb1507), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_101949.5

ORF Insert Sequence:

ATGGCGAACT TGGGAGGCGG TGCGGAAGCA CATGCCAGGT TCAAGCAGTA TGAGTACAGA 
GCCAATTCTA GTCTGGTGTT AACTACTGAT AATAGACCTC GGGATACTCA TGAGCCTACA 
GGAGAACCGG AAACTCTGTG GGGGAAAATC GATCCGAGGT CTTTTGGTGA CCGTGTTGCT 
AAGGGAAGGC CTCAGGAGTT GGAGGATAAG CTGAAAAAGT CTAAGAAGAA GGAACGTGAT 
GTTGTAGATG ATATGGTAAA TATTAGGCAG AGTAAGAGGC GGCGTCTAAG GGAAGAGAGT 
GTGCTGACTG ATACTGATGA TGCGGTTTAT CAGCCGAAGA CTAAGGAGAC GAGGGCTGCT 
TATGAAGCCA TGCTTGGTCT TATCCAGAAG CAGTTGGGTG GGCAACCTCC GAGTATTGTT 
AGTGGTGCAG CGGATGAGAT TTTGGCTGTT CTCAAGAATG ACGCATTTAG AAACCCTGAG 
AAAAAGATGG AGATTGAGAA GTTGCTGAAC AAGATTGAAA ACCATGAGTT TGATCAGCTT 
GTTTCCATTG GAAAGTTAAT CACGGACTTC CAAGAGGGTG GTGATTCTGG TGGTGGTAGA 
GCTAATGATG ATGAAGGGCT TGATGATGAT TTGGGCGTGG CTGTTGAATT TGAGGAGAAT 
GAAGAAGATG ATGAGGAAAG CGATCCTGAT ATGGTTGAAG AGGATGATGA TGAGGAGGAT 
GATGAGCCTA CCAGAACTGG AGGCATGCAG GTTGATGCAG GGATAAATGA TGAGGATGCA 
GGAGATGCCA ATGAGGGCAC AAATCTTAAT GTTCAAGACA TTGATGCATA TTGGCTCCAG 
AGAAAGATCT CTCAAGCGTA TGAACAGCAG ATTGATCCAC AACAATGCCA GGTACTCGCA 
GAAGAGCTAC TAAAGATACT CGCTGAAGGG GATGATAGGG TTGTTGAGGA CAAGCTGCTT 
ATGCACCTTC AGTACGAAAA ATTTAGCCTT GTCAAGTTTC TGCTGCGTAA CCGTCTTAAA 
GTTGTGTGGT GCACTCGTTT GGCTAGGGCA GAAGACCAGG AAGAGAGGAA TAGAATCGAG 
GAGGAGATGA GGGGGTTAGG CCCAGAGTTG ACAGCAATTG TGGAGCAGTT GCATGCAACA 
AGAGCAACAG CGAAAGAGAG GGAGGAGAAC CTGCAAAAAA GTATTAACGA GGAAGCCCGG 
CGTTTGAAGG ACGAAACTGG TGGAGATGGA GGCAGAGGCA GGAGAGATGT TGCTGATAGA 
GATTCAGAGA GTGGGTGGGT GAAGGGTCAG CGTCAGATGC TGGACCTGGA GAGCTTGGCT 
TTTGACCAAG GTGGTCTTCT GATGGCGAAT AAGAAGTGTG ACCTCCCCCC TGGCTCTTAC 
AGAAGTCATG GCAAGGGATA TGATGAAGTT CATGTGCCTT GGGTTTCTAA AAAGGTTGAT 
AGGAATGAGA AGCTAGTGAA GATTACGGAA ATGCCTGATT GGGCGCAACC GGCTTTTAAG 
GGAATGCAAC AGTTGAACAG GGTTCAGAGC AAAGTGTATG ACACTGCGCT TTTCAAGGCA 
GAGAATATTC TTCTTTGTGC TCCAACTGGT GCTGGGAAAA CCAATGTTGC TATGCTCACC 
ATACTGCAGC AACTTGAAAT GAATAGGAAC ACAGATGGAA CTTATAACCA TGGAGATTAC 
AAAATTGTCT ATGTTGCACC CATGAAAGCC CTTGTTGCTG AAGTTGTAGG TAATTTGTCT 
AATCGTCTGA AGGATTATGG AGTTATTGTG AGAGAACTCA GTGGGGATCA GTCCCTAACC 
GGAAGAGAAA TTGAAGAGAC TCAGATAATT GTTACAACAC CAGAGAAATG GGATATCATC 
ACTAGGAAGT CTGGAGACCG AACTTATACT CAGCTTGTGA GACTTCTTAT AATTGATGAA 
ATTCATCTCC TTCATGACAA TCGAGGGCCT GTGCTTGAAA GCATTGTTGC TAGGACTCTT 
AGGCAAATTG AAACCACGAA AGAGAATATT CGTCTGGTTG GTTTATCTGC TACATTGCCA 
AATTATGAAG ACGTGGCCTT ATTTTTGCGG GTTGATCTTA AGAAAGGTTT GTTTAAATTT 
GACCGCAGCT ACAGACCCGT GCCTCTCCAT CAGCAGTATA TTGGAATCAG TGTGAAGAAA 
CCGTTGCAGA GGTTCCAGTT GATGAATGAT CTTTGTTATC AGAAAGTATT GGCTGGTGCT 
GGAAAGCACC AGGTCCTTAT TTTTGTTCAT TCAAGGAAGG AAACATCAAA AACTGCTAGA 
GCAATAAGGG ACACTGCAAT GGCGAACGAT ACCCTCAGCA GATTCTTGAA GGAAGATAGT 
GTAACTCGTG ACGTTCTCCA TAGTCATGAA GACATTGTTA AGAATAGTGA CCTAAAAGAT 
ATTCTGCCTT ACGGTTTTGC AATTCATCAT GCTGGGTTAT CAAGGGGTGA TCGTGAGATT 
GTTGAGACTC TTTTTTCTCA GGGGCATGTG CAAGTCTTGG TTTCCACAGC AACTCTCGCA 
TGGGGTGTGA ATCTGCCTGC ACATACCGTA ATTATAAAAG GAACCCAGGT GTATAATCCT 
GAAAAAGGGG CGTGGATGGA GCTGAGTCCT CTAGATGTTA TGCAGATGCT TGGTCGTGCT 
GGAAGACCTC AGTATGATCA ACATGGGGAG GGTATAATTA TAACTGGCTA CAGCGAGCTG 
CAATATTATC TTTCTTTGAT GAATGAGCAA CTACCTATTG AAAGCCAGTT TATTTCCAAA 
CTTGCTGATC AGCTTAATGC TGAAATTGTG CTTGGAACCG TTCAGAATGC TAGAGAGGCT 
TGCCATTGGC TTGGTTACAC GTATTTGTAT ATCCGTATGG TTCGTAATCC AACGTTGTAT 
GGCTTAGCGC CTGATGCCCT TGCAAAAGAC GTAGTTTTGG AGGAGAGAAG AGCTGACCTG 
ATACATTCGG CTGCTACTAT CTTGGACAAA AACAACTTGG TTAAGTATGA CAGGAAAAGT 
GGATACTTCC AAGTTACAGA TTTGGGACGC ATAGCAAGCT ACTATTACAT AACCCATGGG 
ACCATAGCTA CATACAATGA GCATTTGAAG CCAACAATGG GTGATATAGA TCTTTATCGT 
CTGTTCTCCC TGAGTGACGA GTTCAAGTAT GTGACTGTTC GGCAAGATGA GAAGATGGAA 
CTGGCGAAAC TTTTGGATCG TGTCCCAATT CCGATCAAGG AAACTTTGGA GGAGCCCAGT 
GCCAAAATAA ATGTATTGCT GCAGGCATAT ATTTCACAGC TAAAGCTTGA GGGTCTTTCT 
TTGACATCTG ACATGGTTTA CATTACGCAG AGTGCTGGAC GTCTTGTGCG AGCTCTTTAT 
GAAATTGTAT TGAAGCGGGG ATGGGCTCAA TTGGCAGAGA AGGCTCTGAA CTTGTCTAAA 
ATGGTAGGGA AGAGGATGTG GAGTGTTCAG ACACCCCTGC GTCAGTTTCA TGGACTCTCC 
AATGATATTT TGATGCAGTT GGAGAAGAAG GATTTGGTGT GGGAGAGGTA CTATGATCTA 
TCCGCACAGG AGCTAGGAGA GCTTATTCGC AGTCCTAAGA TGGGCAAGCC GCTTCACAAG 
TTCATTCACC AGTTCCCAAA AGTGACTCTT TCTGCACATG TTCAGCCTAT TACTCGCACT 
GTTCTGAATG TGGAGCTTAC AGTTACACCT GATTTCCTGT GGGACGAGAA AATTCATAAA 
TATGTTGAGC CGTTTTGGAT AATTGTGGAA GACAATGATG GTGAAAAGAT CCTTCATCAC 
GAGTATTTTC TTTTGAAGAA GCAGTATATT GATGAGGATC ACACTTTGCA CTTCACTGTG 
CCAATTTTTG AACCGTTGCC ACCTCAGTAT TTTGTTCGAG TGGTTTCAGA CAAGTGGCTT 
GGCTCGGAGA CTGTGTTGCC TGTATCTTTC AGGCATCTTA TTCTTCCAGA GAAGTATCCA 
CCTCCGACAG AGTTACTGGA CTTGCAACCT CTCCCAGTGA CGGCGCTAAG GAATCCGAAT 
TATGAGATAC TCTATCAGGA TTTCAAGCAT TTCAATCCAG TGCAGACTCA GGTCTTTACT 
GTTTTGTATA ACACTAACGA CAATGTGTTG GTTGCTGCTC CAACGGGAAG TGGGAAAACT 
ATTTGTGCGG AGTTCGCTAT TTTGAGGAAT CATCATGAAG GACCTGATGC TACAATGCGT 
GTTGTTTATA TTGCACCACT TGAGGCTATT GCCAAGGAGC AGTTTCGTAT CTGGGAGGGG 
AAGTTTGGGA AGGGTCTTGG TTTACGGGTT GTTGAGCTAA CTGGGGAGAC AGCATTGGAT 
CTTAAGCTGC TTGAGAAAGG CCAGATAATC ATAAGTACTC CTGAGAAATG GGATGCTCTT 
TCCCGCAGGT GGAAACAGAG AAAATATGTT CAACAAGTTA GTCTTTTCAT CGTCGACGAA 
CTCCATCTAA TTGGAGGTCA ACATGGTCCA GTGTTGGAGG TAATCGTTTC CAGAATGAGA 
TATATTTCAA GTCAGGTCAT TAATAAGATC AGGATTGTTG CGCTATCCAC TTCGCTTGCA 
AATGCAAAAG ATCTTGGGGA GTGGATTGGG GCCAGTTCCC ACGGCCTTTT CAACTTCCCT 
CCCGGAGTCC GTCCTGTTCC ACTGGAGATC CACATTCAGG GAGTGGATAT ATCCAGTTTT 
GAAGCCAGGA TGCAGGCGAT GACCAAACCA ACCTACACCG CCATAGTTCA GCACGCCAAG 
AACAAGAAGC CGGCTATTGT TTTCGTCCCA ACCCGTAAAC ATGTCCGCTT GACTGCTGTG 
GATCTGATGG CTTATTCTCA CATGGATAAC CCGCAGAGTC CTGATTTCTT GTTAGGGAAG 
CTGGAAGAAC TGGACCCTTT TGTTGAACAA ATCCGCGAGG AAACACTGAA GGAGACACTG 
TGTCATGGTA TTGGATACTT GCATGAGGGT TTAAGCAGTC TGGATCAGGA GATTGTGACA 
CAGCTCTTTG AAGCTGGACG GATTCAGGTT TGTGTGATGA GCAGTTCATT GTGTTGGGGC 
ACTCCATTAA CTGCGCATTT GGTGGTCGTA ATGGGAACGC AGTATTACGA TGGGAGGGAA 
AATTCACATT CTGATTATCC AGTCCCTGAT CTGCTTCAGA TGATGGGGCG TGCTAGCCGA 
CCACTTCTTG ACAATGCCGG GAAGTGTGTG ATCTTTTGTC ATGCACCGCG GAAGGAGTAT 
TACAAGAAGT TTTTGTACGA AGCCTTCCCT GTGGAAAGTC AGCTCCAGCA TTTCTTGCAT 
GATAATTTCA ACGCAGAAGT GGTTGCTGGG GTTATAGAGA ACAAGCAAGA TGCCGTGGAT 
TATCTTACAT GGACTTTCAT GTACAGGAGG CTTCCCCAGA ACCCTAACTA CTACAATCTC 
CAAGGTGTTA GCCACAGGCA TTTGTCTGAT CACCTATCAG AGCTGGTTGA GAACACCTTG 
TCTGATCTTG AAGCCAGTAA ATGCATTGAG GTAGAGGATG AGATGGAACT CTCTCCTCTC 
AACCTTGGTA TGATTGCATC CTATTATTAC ATCAGTTATA CCACCATTGA GCGCTTCAGT 
TCACTGTTGT CTTCTAAAAC CAAGATGAAG GGACTTCTTG AAATCTTAAC ATCGGCTTCA 
GAGTATGACA TGATTCCTAT ACGGCCTGGC GAAGAGGACA CCGTTCGAAG ATTAATCAAT 
CACCAGAGGT TCTCTTTTGA AAACCCAAAA TGCACAGACC CTCATGTGAA GGCAAACGCG 
CTTCTTCAGG CGCATTTCTC AAGGCAAAAC ATTGGCGGGA ACTTGGCAAT GGATCAGCGT 
GATGTCCTTC TATCTGCAAC CAGACTTCTT CAAGCGATGG TCGATGTGAT ATCAAGCAAT 
GGCTGGCTGA ATCTTGCTCT GCTAGCTATG GAAGTAAGCC AGATGGTGAC TCAAGGCATG 
TGGGAGCGGG ACTCCATGCT TCTGCAGCTC CCTCACTTCA CAAAAGACTT GGCCAAGAGG 
TGCCAGGAGA ATCCAGGGAA AAACATTGAA ACCGTATTCG ATCTTGTGGA GATGGAGGAT 
GAAGAACGCC AAGAGCTTCT CAAGATGTCA GACGCTCAGC TTCTAGACAT TGCCAGATTC 
TGCAACCGCT TCCCAAACAT CGACCTTACT TACGAGATTG TGGGCAGTGA AGAGGTGAAC 
CCAGGGAAAG AGGTTACATT GCAAGTGATG CTTGAGAGAG ACATGGAGGG GAGGACAGAA 
GTGGGACCTG TGGATTCACT GAGGTATCCC AAGACCAAAG AAGAAGGATG GTGGCTGGTC 
GTGGGCGACA CCAAGACAAA CCAGTTGCTG GCAATCAAGC GTGTCTCATT GCAACGGAAA 
GTGAAGGTGA AGCTGGATTT CACAGCACCG AGTGAACCAG GAGAGAAGTC ATACACACTA 
TACTTCATGT GTGACTCTTA CTTGGGGTGT GACCAAGAGT ACTCGTTCTC TGTTGATGTC 
AAAGGATCCG GCGCTGGAGA TCGCATGGAA GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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emb1507 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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