SNRNP200 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SNRNP200
Entrez Gene ID	23020
Full Name	small nuclear ribonucleoprotein U5 subunit 200
Synonyms	ASCC3L1,BRR2,HELIC2,RP33,U5-200KD
General protein information	Preferred Names small nuclear ribonucleoprotein U5 subunit 200 Names U5 small nuclear ribonucleoprotein 200 kDa helicase BRR2 homolog U5 snRNP-specific 200 kDa protein activating signal cointegrator 1 complex subunit 3-like 1 bad response to refrigeration 2 homolog small nuclear ribonucleoprotein 200kDa (U5) small nuclear ribonucleoprotein, U5 200kDa subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q11.2
Summary	Pre-mRNA splicing is catalyzed by the spliceosome, a complex of specialized RNA and protein subunits that removes introns from a transcribed pre-mRNA segment. The spliceosome consists of small nuclear RNA proteins (snRNPs) U1, U2, U4, U5 and U6, together with approximately 80 conserved proteins. U5 snRNP contains nine specific proteins. This gene encodes one of the U5 snRNP-specific proteins. This protein belongs to the DEXH-box family of putative RNA helicases. It is a core component of U4/U6-U5 snRNPs and appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. Mutations in this gene cause autosomal-dominant retinitis pigmentosa. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined. [provided by RefSeq, Mar 2010].
Disorder MIM:	601664
Disorder Html:	Retinitis pigmentosa 33, 610359 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017003603.1	XP_016859092.1	U5 small nuclear ribonucleoprotein 200 kDa helicase isoform X1
NM_014014.4	NP_054733.2	U5 small nuclear ribonucleoprotein 200 kDa helicase
NM_014014.5	NP_054733.2	U5 small nuclear ribonucleoprotein 200 kDa helicase

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	brr2	NP_593346.1
Canis lupus familiaris (dog)	SNRNP200	XP_532949.2
Bos taurus (cattle)	SNRNP200	NP_001193092.1
Caenorhabditis elegans (roundworm)	snrp-200	NP_496710.1
Kluyveromyces lactis	KLLA0C17798g	XP_452995.1
Arabidopsis thaliana (thale cress)	AT2G42270	NP_181756.1
Pan troglodytes (chimpanzee)	SNRNP200	XP_001147672.1
Saccharomyces cerevisiae (baker's yeast)	BRR2	NP_011099.1
Eremothecium gossypii	AGOS_AGR113W	NP_986779.1
Neurospora crassa	NCU02685	XP_965458.1
Xenopus tropicalis (tropical clawed frog)	snrnp200	XP_002932581.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001519	XP_321605.3
Magnaporthe oryzae (rice blast fungus)	MGG_15536	XP_003713506.1
Homo sapiens (human)	SNRNP200	NP_054733.2
	SNRNP200	XP_001098299.2
Mus musculus (house mouse)	Snrnp200	NP_796188.2
Rattus norvegicus (Norway rat)	Snrnp200	NP_001032855.2
Danio rerio (zebrafish)	snrnp200	NP_001116729.1
Drosophila melanogaster (fruit fly)	l(3)72Ab	NP_648818.3
Arabidopsis thaliana (thale cress)	emb1507	NP_001185050.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of a human catalytic step I spliceosome.
Zhan X, Yan C, Zhang X, Lei J, Shi Y
Science (New York, N.Y.)(2018 Jan)

Variability in clinical phenotypes of PRPF8-linked autosomal dominant retinitis pigmentosa correlates with differential PRPF8/SNRNP200 interactions.
Escher P, Passarin O, Munier FL, Tran VH, Vaclavik V
Ophthalmic genetics39(1)80-86(2018 Jan-Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Biochemical and biophysical analyses revealed that an intrinsically disordered region of FBP21 binds to an extended surface of the C-terminal Sec63 unit of Brr2. Additional contacts in the C-terminal helicase cassette are required for allosteric inhibition of Brr2 helicase activity.
Title: A new role for FBP21 as regulator of Brr2 helicase activity.

This work identifies a novel immunoregulatory role of the spliceosomal SNRNP200 helicase as an RNA sensor and TBK1 adaptor for the activation of IRF3-mediated antiviral innate response
Title: Spliceosome SNRNP200 Promotes Viral RNA Sensing and IRF3 Activation of Antiviral Response.

Results show that SNW1 directly associates with EFTUD2 and SNRNP200 and that disruption of SNW1 association with these proteins promotes the apoptosis of breast cancer cells.
Title: Inhibition of SNW1 association with spliceosomal proteins promotes apoptosis in breast cancer cells.

Our data suggest that BRR2 is an important factor in 5'-splice-site recognition and that the retinitis pigmentosa linked mutations c.3260C>T (p.S1087L) and c.3269G>T (p.R1090L) affect this BRR2 function.
Title: Retinitis pigmentosa mutations of SNRNP200 enhance cryptic splice-site recognition.

Mutations in SNRNP200 cause 1.6% of autosomal dominant retinitis pigmentosa.
Title: Mutations in the small nuclear riboprotein 200 kDa gene (SNRNP200) cause 1.6% of autosomal dominant retinitis pigmentosa.

The following SNRNP200 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SNRNP200 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18366	NM_014014.5 Latest version!	Homo sapiens small nuclear ribonucleoprotein U5 subunit 200 (SNRNP200), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu18366	XM_017003603.1 Latest version!	Homo sapiens small nuclear ribonucleoprotein U5 subunit 200 (SNRNP200), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
View Old Accession Versions >>
OHu18366	NM_014014.4	Homo sapiens small nuclear ribonucleoprotein U5 subunit 200 (SNRNP200), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SNRNP200 ( NM_014014.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_054733.2 Homo sapiens small nuclear ribonucleoprotein U5 subunit 200 (SNRNP200), mRNA.

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CloneID	OHu18366
Clone ID Related Accession (Same CDS sequence)	XM_017003603.1 , NM_014014.4 , NM_014014.5
Accession Version	NM_014014.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	U5 small nuclear ribonucleoprotein 200 kDa helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP873066.1, DB067751.1, AY572488.1, AB018331.2 and AW190145.1. This sequence is a reference standard in the RefSeqGene project. On Mar 26, 2011 this sequence version replaced NM_014014.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.60324.1, SRR1803613.85420.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATG TAACCGCCCG TAGTCTGCAA TACGAGTACA AGGCGAACTC GAATCTTGTG 
CTCCAAGCTG ACCGTTCTCT CATTGACCGG ACCCGCCGGG ATGAACCCAC AGGAGAGGTG 
CTGTCCCTTG TTGGGAAGCT GGAGGGCACC CGTATGGGAG ACAAGGCTCA ACGGACCAAA 
CCGCAGATGC AGGAGGAAAG AAGAGCCAAG CGAAGAAAGC GTGATGAGGA CCGGCATGAC 
ATCAACAAGA TGAAGGGTTA TACTCTGCTG TCGGAGGGCA TTGATGAGAT GGTGGGCATC 
ATCTACAAGC CCAAAACTAA AGAGACTCGG GAGACCTATG AGGTGCTACT CAGCTTCATC 
CAGGCTGCTC TTGGGGACCA GCCACGTGAT ATCCTTTGTG GGGCAGCTGA TGAAGTTCTA 
GCTGTTCTAA AGAATGAAAA GCTGCGGGAC AAGGAAAGGC GAAAGGAGAT TGACCTGCTG 
CTGGGTCAAA CAGATGATAC CAGATACCAT GTGCTAGTGA ACCTGGGCAA AAAGATCACA 
GACTATGGTG GAGATAAGGA AATCCAAAAT ATGGATGACA ACATTGATGA GACATACGGT 
GTGAATGTGC AGTTTGAGTC TGATGAGGAG GAAGGTGATG AAGACGTATA CGGGGAGGTT 
CGAGAAGAGG CATCTGATGA TGACATGGAA GGGGACGAGG CTGTCGTGCG CTGCACCCTC 
TCGGCTAATC TCGTAGCCTC AGGTGAACTG ATGAGTTCCA AGAAGAAGGA TTTGCACCCT 
CGGGATATTG ATGCATTTTG GCTGCAGCGG CAGCTCAGTC GTTTCTATGA TGATGCCATC 
GTGTCGCAGA AGAAGGCAGA TGAAGTATTG GAGATTTTGA AGACGGCCAG TGATGATCGG 
GAATGTGAAA ATCAGCTGGT TCTGCTGCTT GGTTTCAACA CCTTTGATTT CATTAAAGTG 
TTGCGGCAGC ACAGGATGAT GATTTTATAC TGTACCTTGC TGGCCAGTGC ACAAAGTGAA 
GCTGAAAAGG AAAGGATTAT GGGAAAGATG GAAGCTGACC CAGAGCTATC CAAGTTCCTC 
TACCAGCTTC ATGAAACCGA GAAGGAGGAT CTGATCCGAG AGGAAAGGTC CCGGAGAGAG 
CGAGTGCGTC AGTCTCGAAT GGACACAGAT CTGGAAACCA TGGATCTCGA CCAGGGTGGA 
GAGGCACTGG CTCCACGGCA GGTTCTGGAC TTGGAGGACC TGGTTTTTAC CCAAGGGAGC 
CACTTTATGG CCAATAAACG CTGTCAGCTT CCTGATGGAT CCTTCCGTCG CCAGCGTAAG 
GGCTATGAAG AGGTGCATGT GCCTGCTCTG AAGCCCAAGC CCTTTGGCTC AGAAGAACAA 
CTGCTTCCAG TGGAAAAGCT GCCAAAGTAT GCCCAGGCTG GGTTTGAGGG CTTCAAAACA 
CTGAATCGGA TCCAGAGTAA GCTCTACCGT GCTGCCCTTG AGACGGATGA GAATCTGCTG 
CTGTGTGCTC CTACTGGTGC TGGGAAGACC AACGTGGCCC TGATGTGCAT GCTCCGAGAG 
ATTGGGAAAC ACATAAACAT GGACGGCACC ATCAATGTGG ATGACTTCAA GATTATCTAC 
ATTGCCCCCA TGCGCTCCTT GGTGCAGGAG ATGGTGGGCA GCTTTGGAAA GCGCCTGGCC 
ACTTATGGCA TCACTGTTGC TGAACTGACT GGGGACCACC AGCTGTGCAA AGAAGAGATC 
AGTGCCACTC AGATCATCGT CTGCACCCCC GAGAAGTGGG ACATCATCAC CCGCAAGGGT 
GGTGAGCGCA CCTACACCCA GCTGGTGCGG CTCATCATTC TGGATGAGAT TCATCTTCTC 
CACGATGACA GAGGTCCTGT CTTAGAAGCT TTAGTGGCCA GGGCCATCCG AAACATTGAG 
ATGACCCAAG AGGATGTCCG ACTCATTGGT CTCAGTGCCA CCCTACCCAA CTATGAAGAT 
GTAGCCACCT TTCTACGTGT TGACCCTGCC AAGGGTCTCT TTTACTTTGA CAACAGCTTC 
CGTCCAGTGC CTCTGGAACA GACATATGTG GGTATCACAG AGAAAAAAGC TATCAAGCGT 
TTCCAGATCA TGAATGAAAT CGTCTATGAA AAAATCATGG AACATGCTGG AAAAAATCAG 
GTGCTGGTGT TTGTCCACTC CCGGAAGGAG ACTGGAAAGA CAGCCAGGGC CATCCGGGAC 
ATGTGCCTAG AAAAGGACAC TCTGGGTCTG TTTCTGAGGG AGGGCTCAGC CTCCACAGAA 
GTCCTGCGAA CAGAAGCTGA GCAGTGCAAG AACCTAGAGC TGAAGGATCT TCTGCCTTAT 
GGCTTTGCTA TTCATCACGC AGGCATGACC AGGGTTGACC GAACACTCGT GGAGGATCTT 
TTTGCTGATA AACATATTCA GGTTTTAGTT TCCACAGCAA CTCTAGCTTG GGGTGTGAAT 
CTCCCTGCAC ATACAGTCAT CATCAAAGGC ACCCAGGTGT ACAGTCCAGA GAAGGGGCGT 
TGGACAGAAC TGGGAGCACT GGACATTCTG CAGATGCTGG GACGTGCCGG AAGACCCCAG 
TATGACACCA AGGGTGAAGG CATACTCATC ACATCTCATG GGGAGCTACA GTACTACCTG 
TCCCTCCTCA ATCAACAACT TCCTATTGAA AGCCAGATGG TTTCAAAGCT TCCTGACATG 
CTCAATGCAG AAATCGTGCT AGGAAATGTC CAGAATGCCA AGGATGCGGT GAACTGGCTG 
GGCTATGCCT ACCTCTATAT CCGAATGCTG CGATCCCCAA CCCTCTATGG CATCTCTCAT 
GATGACCTCA AGGGAGATCC CCTGCTGGAC CAGCGCCGAC TAGATCTGGT TCATACAGCT 
GCCCTGATGC TGGACAAGAA CAATCTGGTC AAGTACGACA AGAAGACGGG CAACTTCCAG 
GTGACAGAAC TGGGCCGTAT AGCCAGCCAC TACTACATCA CCAATGATAC AGTGCAGACT 
TACAACCAGC TGCTGAAGCC CACCCTGAGT GAGATTGAGC TTTTCAGGGT CTTCTCATTG 
TCCTCTGAGT TCAAGAACAT CACAGTGAGA GAGGAGGAGA AGCTGGAGCT GCAGAAGTTG 
CTGGAGAGGG TGCCTATCCC TGTAAAGGAG AGCATTGAGG AACCCAGTGC TAAGATCAAC 
GTTCTTCTGC AAGCCTTCAT CTCACAGCTG AAATTGGAGG GCTTTGCACT GATGGCTGAC 
ATGGTGTATG TCACACAGTC GGCTGGCCGG TTGATGCGAG CGATATTTGA AATTGTCCTG 
AACCGAGGTT GGGCACAGCT TACAGACAAG ACCCTGAACC TCTGCAAGAT GATCGACAAA 
CGCATGTGGC AGTCCATGTG TCCTCTGCGC CAGTTCCGGA AACTCCCTGA GGAAGTAGTG 
AAGAAGATTG AGAAGAAGAA TTTCCCCTTT GAGCGTCTGT ACGACCTGAA TCATAATGAG 
ATTGGGGAGC TTATCCGCAT GCCAAAGATG GGGAAGACCA TCCACAAATA TGTCCATCTG 
TTTCCCAAGT TGGAGTTGTC AGTGCACCTG CAGCCTATCA CACGCTCCAC CCTGAAGGTG 
GAGCTGACCA TCACGCCAGA CTTCCAGTGG GATGAAAAGG TGCATGGTTC ATCCGAGGCT 
TTTTGGATTC TGGTGGAGGA TGTGGACAGC GAGGTGATTC TGCACCATGA GTATTTTCTC 
CTCAAGGCCA AGTACGCCCA GGACGAGCAC CTCATTACAT TCTTCGTGCC TGTCTTTGAA 
CCGCTGCCCC CTCAGTACTT CATCCGAGTG GTGTCTGACC GCTGGCTCTC TTGTGAGACC 
CAGCTGCCTG TCTCCTTCCG GCACCTGATC TTGCCGGAGA AGTACCCCCC TCCAACCGAA 
CTTTTGGACC TGCAGCCCTT GCCCGTGTCT GCTCTGAGAA ACAGTGCCTT TGAGAGTCTT 
TACCAAGATA AATTTCCTTT CTTCAATCCC ATCCAGACCC AGGTGTTTAA CACTGTATAC 
AACAGTGACG ACAACGTGTT TGTGGGGGCC CCCACGGGCA GCGGGAAGAC TATTTGTGCA 
GAGTTTGCCA TCCTGCGAAT GCTGCTGCAG AGCTCGGAGG GGCGCTGTGT GTACATCACC 
CCCATGGAGG CCCTGGCAGA GCAGGTATAC ATGGACTGGT ACGAGAAGTT CCAGGACAGG 
CTCAACAAGA AGGTGGTACT CCTGACAGGC GAGACCAGCA CAGACCTGAA GCTGCTGGGC 
AAAGGGAACA TTATCATCAG CACCCCTGAG AAGTGGGACA TACTTTCCCG GCGATGGAAG 
CAGCGCAAGA ACGTGCAGAA CATCAACCTC TTCGTGGTGG ATGAGGTCCA CCTTATCGGG 
GGCGAGAATG GGCCTGTCTT AGAAGTGATC TGCTCCCGAA TGCGCTACAT CTCCTCCCAG 
ATTGAGCGGC CCATTCGCAT TGTGGCACTC AGCTCTTCGC TCTCCAATGC CAAGGATGTG 
GCCCACTGGC TGGGCTGCAG TGCCACCTCC ACCTTCAACT TCCATCCCAA TGTGCGTCCC 
GTCCCCTTGG AGCTGCACAT CCAGGGCTTC AACATCAGCC ATACACAAAC CCGCCTGCTC 
TCCATGGCCA AGCCTGTGTA CCATGCTATC ACCAAGCACT CGCCCAAGAA GCCTGTCATT 
GTCTTTGTGC CGTCTCGCAA GCAGACCCGC CTCACTGCCA TTGACATCCT CACCACCTGT 
GCAGCAGACA TCCAACGGCA GAGGTTCTTG CACTGCACCG AGAAGGATCT GATTCCGTAC 
CTGGAGAAGC TAAGTGACAG CACGCTCAAG GAAACGCTGC TAAATGGGGT GGGCTACCTG 
CATGAGGGGC TCAGCCCCAT GGAGCGACGC CTGGTGGAGC AGCTCTTCAG CTCAGGGGCT 
ATCCAGGTGG TGGTGGCTTC TCGGAGTCTC TGCTGGGGCA TGAACGTGGC TGCCCACCTG 
GTAATCATCA TGGATACCCA GTACTACAAT GGCAAGATCC ACGCCTATGT GGATTACCCC 
ATCTATGACG TGCTTCAGAT GGTGGGCCAC GCCAACCGCC CTTTGCAGGA CGATGAGGGG 
CGCTGTGTCA TCATGTGTCA GGGCTCCAAG AAGGATTTCT TCAAGAAGTT CTTATATGAG 
CCATTGCCAG TAGAATCTCA CCTGGACCAC TGTATGCATG ACCACTTCAA TGCTGAGATC 
GTCACCAAGA CCATTGAGAA CAAGCAGGAT GCTGTGGACT ACCTCACCTG GACCTTTCTG 
TACCGCCGCA TGACACAGAA CCCCAATTAC TACAACCTGC AGGGCATCTC CCATCGTCAC 
TTGTCGGACC ACTTGTCAGA GCTGGTGGAG CAGACCCTGA GTGACCTGGA GCAGTCCAAG 
TGCATCAGCA TCGAGGACGA GATGGACGTG GCGCCTCTGA ACCTAGGCAT GATCGCCGCC 
TACTATTACA TCAACTACAC CACCATTGAG CTCTTCAGCA TGTCCCTCAA TGCCAAGACC 
AAGGTGCGAG GGCTTATCGA GATCATCTCC AATGCAGCAG AGTATGAGAA CATTCCCATC 
CGGCACCATG AAGACAATCT CCTGAGGCAG TTGGCTCAGA AGGTCCCCCA CAAGCTGAAT 
AACCCTAAGT TCAATGATCC GCACGTCAAG ACCAACCTGC TCCTGCAGGC TCACTTGTCT 
CGCATGCAGC TGAGTGCTGA GTTGCAGTCA GATACGGAGG AAATCCTTAG TAAGGCAATC 
CGGCTCATCC AGGCCTGCGT GGATGTCCTT TCCAGCAATG GGTGGCTCAG CCCTGCTCTG 
GCAGCTATGG AACTGGCCCA GATGGTCACC CAAGCCATGT GGTCCAAGGA CTCATACCTG 
AAGCAGCTGC CACACTTCAC CTCTGAGCAT ATCAAACGTT GCACAGACAA GGGAGTGGAG 
AGTGTTTTCG ACATCATGGA GATGGAGGAT GAAGAACGGA ACGCGTTGCT TCAGCTGACT 
GACAGCCAGA TTGCAGATGT GGCTCGCTTT TGTAACCGCT ACCCTAATAT CGAACTATCT 
TATGAGGTGG TAGATAAGGA CAGCATCCGC AGTGGCGGGC CAGTTGTGGT GCTGGTGCAG 
CTGGAGCGAG AGGAGGAAGT CACAGGCCCT GTCATTGCGC CTCTCTTCCC GCAGAAACGT 
GAAGAGGGCT GGTGGGTGGT GATTGGAGAT GCCAAGTCCA ATAGCCTCAT CTCCATCAAG 
AGGCTGACCT TGCAGCAGAA GGCCAAGGTG AAGTTGGACT TTGTGGCCCC AGCCACTGGT 
GCCCACAACT ACACTCTGTA CTTCATGAGT GACGCTTACA TGGGATGTGA CCAGGAGTAC 
AAATTCAGCG TGGATGTGAA AGAAGCTGAG ACAGACAGTG ATTCAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054733.2
CDS	133..6543
Translation	MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTKPQMQEERRAKRRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFIQAALGDQPRDILCGAADEVLAVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKITDYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTLSANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFLYQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD

Target ORF information:

RefSeq Version	NM_014014.4
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein U5 subunit 200 (SNRNP200), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014014.4

ORF Insert Sequence:

ATGGCGGATG TAACCGCCCG TAGTCTGCAA TACGAGTACA AGGCGAACTC GAATCTTGTG 
CTCCAAGCTG ACCGTTCTCT CATTGACCGG ACCCGCCGGG ATGAACCCAC AGGAGAGGTG 
CTGTCCCTTG TTGGGAAGCT GGAGGGCACC CGTATGGGAG ACAAGGCTCA ACGGACCAAA 
CCGCAGATGC AGGAGGAAAG AAGAGCCAAG CGAAGAAAGC GTGATGAGGA CCGGCATGAC 
ATCAACAAGA TGAAGGGTTA TACTCTGCTG TCGGAGGGCA TTGATGAGAT GGTGGGCATC 
ATCTACAAGC CCAAAACTAA AGAGACTCGG GAGACCTATG AGGTGCTACT CAGCTTCATC 
CAGGCTGCTC TTGGGGACCA GCCACGTGAT ATCCTTTGTG GGGCAGCTGA TGAAGTTCTA 
GCTGTTCTAA AGAATGAAAA GCTGCGGGAC AAGGAAAGGC GAAAGGAGAT TGACCTGCTG 
CTGGGTCAAA CAGATGATAC CAGATACCAT GTGCTAGTGA ACCTGGGCAA AAAGATCACA 
GACTATGGTG GAGATAAGGA AATCCAAAAT ATGGATGACA ACATTGATGA GACATACGGT 
GTGAATGTGC AGTTTGAGTC TGATGAGGAG GAAGGTGATG AAGACGTATA CGGGGAGGTT 
CGAGAAGAGG CATCTGATGA TGACATGGAA GGGGACGAGG CTGTCGTGCG CTGCACCCTC 
TCGGCTAATC TCGTAGCCTC AGGTGAACTG ATGAGTTCCA AGAAGAAGGA TTTGCACCCT 
CGGGATATTG ATGCATTTTG GCTGCAGCGG CAGCTCAGTC GTTTCTATGA TGATGCCATC 
GTGTCGCAGA AGAAGGCAGA TGAAGTATTG GAGATTTTGA AGACGGCCAG TGATGATCGG 
GAATGTGAAA ATCAGCTGGT TCTGCTGCTT GGTTTCAACA CCTTTGATTT CATTAAAGTG 
TTGCGGCAGC ACAGGATGAT GATTTTATAC TGTACCTTGC TGGCCAGTGC ACAAAGTGAA 
GCTGAAAAGG AAAGGATTAT GGGAAAGATG GAAGCTGACC CAGAGCTATC CAAGTTCCTC 
TACCAGCTTC ATGAAACCGA GAAGGAGGAT CTGATCCGAG AGGAAAGGTC CCGGAGAGAG 
CGAGTGCGTC AGTCTCGAAT GGACACAGAT CTGGAAACCA TGGATCTCGA CCAGGGTGGA 
GAGGCACTGG CTCCACGGCA GGTTCTGGAC TTGGAGGACC TGGTTTTTAC CCAAGGGAGC 
CACTTTATGG CCAATAAACG CTGTCAGCTT CCTGATGGAT CCTTCCGTCG CCAGCGTAAG 
GGCTATGAAG AGGTGCATGT GCCTGCTCTG AAGCCCAAGC CCTTTGGCTC AGAAGAACAA 
CTGCTTCCAG TGGAAAAGCT GCCAAAGTAT GCCCAGGCTG GGTTTGAGGG CTTCAAAACA 
CTGAATCGGA TCCAGAGTAA GCTCTACCGT GCTGCCCTTG AGACGGATGA GAATCTGCTG 
CTGTGTGCTC CTACTGGTGC TGGGAAGACC AACGTGGCCC TGATGTGCAT GCTCCGAGAG 
ATTGGGAAAC ACATAAACAT GGACGGCACC ATCAATGTGG ATGACTTCAA GATTATCTAC 
ATTGCCCCCA TGCGCTCCTT GGTGCAGGAG ATGGTGGGCA GCTTTGGAAA GCGCCTGGCC 
ACTTATGGCA TCACTGTTGC TGAACTGACT GGGGACCACC AGCTGTGCAA AGAAGAGATC 
AGTGCCACTC AGATCATCGT CTGCACCCCC GAGAAGTGGG ACATCATCAC CCGCAAGGGT 
GGTGAGCGCA CCTACACCCA GCTGGTGCGG CTCATCATTC TGGATGAGAT TCATCTTCTC 
CACGATGACA GAGGTCCTGT CTTAGAAGCT TTAGTGGCCA GGGCCATCCG AAACATTGAG 
ATGACCCAAG AGGATGTCCG ACTCATTGGT CTCAGTGCCA CCCTACCCAA CTATGAAGAT 
GTAGCCACCT TTCTACGTGT TGACCCTGCC AAGGGTCTCT TTTACTTTGA CAACAGCTTC 
CGTCCAGTGC CTCTGGAACA GACATATGTG GGTATCACAG AGAAAAAAGC TATCAAGCGT 
TTCCAGATCA TGAATGAAAT CGTCTATGAA AAAATCATGG AACATGCTGG AAAAAATCAG 
GTGCTGGTGT TTGTCCACTC CCGGAAGGAG ACTGGAAAGA CAGCCAGGGC CATCCGGGAC 
ATGTGCCTAG AAAAGGACAC TCTGGGTCTG TTTCTGAGGG AGGGCTCAGC CTCCACAGAA 
GTCCTGCGAA CAGAAGCTGA GCAGTGCAAG AACCTAGAGC TGAAGGATCT TCTGCCTTAT 
GGCTTTGCTA TTCATCACGC AGGCATGACC AGGGTTGACC GAACACTCGT GGAGGATCTT 
TTTGCTGATA AACATATTCA GGTTTTAGTT TCCACAGCAA CTCTAGCTTG GGGTGTGAAT 
CTCCCTGCAC ATACAGTCAT CATCAAAGGC ACCCAGGTGT ACAGTCCAGA GAAGGGGCGT 
TGGACAGAAC TGGGAGCACT GGACATTCTG CAGATGCTGG GACGTGCCGG AAGACCCCAG 
TATGACACCA AGGGTGAAGG CATACTCATC ACATCTCATG GGGAGCTACA GTACTACCTG 
TCCCTCCTCA ATCAACAACT TCCTATTGAA AGCCAGATGG TTTCAAAGCT TCCTGACATG 
CTCAATGCAG AAATCGTGCT AGGAAATGTC CAGAATGCCA AGGATGCGGT GAACTGGCTG 
GGCTATGCCT ACCTCTATAT CCGAATGCTG CGATCCCCAA CCCTCTATGG CATCTCTCAT 
GATGACCTCA AGGGAGATCC CCTGCTGGAC CAGCGCCGAC TAGATCTGGT TCATACAGCT 
GCCCTGATGC TGGACAAGAA CAATCTGGTC AAGTACGACA AGAAGACGGG CAACTTCCAG 
GTGACAGAAC TGGGCCGTAT AGCCAGCCAC TACTACATCA CCAATGATAC AGTGCAGACT 
TACAACCAGC TGCTGAAGCC CACCCTGAGT GAGATTGAGC TTTTCAGGGT CTTCTCATTG 
TCCTCTGAGT TCAAGAACAT CACAGTGAGA GAGGAGGAGA AGCTGGAGCT GCAGAAGTTG 
CTGGAGAGGG TGCCTATCCC TGTAAAGGAG AGCATTGAGG AACCCAGTGC TAAGATCAAC 
GTTCTTCTGC AAGCCTTCAT CTCACAGCTG AAATTGGAGG GCTTTGCACT GATGGCTGAC 
ATGGTGTATG TCACACAGTC GGCTGGCCGG TTGATGCGAG CGATATTTGA AATTGTCCTG 
AACCGAGGTT GGGCACAGCT TACAGACAAG ACCCTGAACC TCTGCAAGAT GATCGACAAA 
CGCATGTGGC AGTCCATGTG TCCTCTGCGC CAGTTCCGGA AACTCCCTGA GGAAGTAGTG 
AAGAAGATTG AGAAGAAGAA TTTCCCCTTT GAGCGTCTGT ACGACCTGAA TCATAATGAG 
ATTGGGGAGC TTATCCGCAT GCCAAAGATG GGGAAGACCA TCCACAAATA TGTCCATCTG 
TTTCCCAAGT TGGAGTTGTC AGTGCACCTG CAGCCTATCA CACGCTCCAC CCTGAAGGTG 
GAGCTGACCA TCACGCCAGA CTTCCAGTGG GATGAAAAGG TGCATGGTTC ATCCGAGGCT 
TTTTGGATTC TGGTGGAGGA TGTGGACAGC GAGGTGATTC TGCACCATGA GTATTTTCTC 
CTCAAGGCCA AGTACGCCCA GGACGAGCAC CTCATTACAT TCTTCGTGCC TGTCTTTGAA 
CCGCTGCCCC CTCAGTACTT CATCCGAGTG GTGTCTGACC GCTGGCTCTC TTGTGAGACC 
CAGCTGCCTG TCTCCTTCCG GCACCTGATC TTGCCGGAGA AGTACCCCCC TCCAACCGAA 
CTTTTGGACC TGCAGCCCTT GCCCGTGTCT GCTCTGAGAA ACAGTGCCTT TGAGAGTCTT 
TACCAAGATA AATTTCCTTT CTTCAATCCC ATCCAGACCC AGGTGTTTAA CACTGTATAC 
AACAGTGACG ACAACGTGTT TGTGGGGGCC CCCACGGGCA GCGGGAAGAC TATTTGTGCA 
GAGTTTGCCA TCCTGCGAAT GCTGCTGCAG AGCTCGGAGG GGCGCTGTGT GTACATCACC 
CCCATGGAGG CCCTGGCAGA GCAGGTATAC ATGGACTGGT ACGAGAAGTT CCAGGACAGG 
CTCAACAAGA AGGTGGTACT CCTGACAGGC GAGACCAGCA CAGACCTGAA GCTGCTGGGC 
AAAGGGAACA TTATCATCAG CACCCCTGAG AAGTGGGACA TACTTTCCCG GCGATGGAAG 
CAGCGCAAGA ACGTGCAGAA CATCAACCTC TTCGTGGTGG ATGAGGTCCA CCTTATCGGG 
GGCGAGAATG GGCCTGTCTT AGAAGTGATC TGCTCCCGAA TGCGCTACAT CTCCTCCCAG 
ATTGAGCGGC CCATTCGCAT TGTGGCACTC AGCTCTTCGC TCTCCAATGC CAAGGATGTG 
GCCCACTGGC TGGGCTGCAG TGCCACCTCC ACCTTCAACT TCCATCCCAA TGTGCGTCCC 
GTCCCCTTGG AGCTGCACAT CCAGGGCTTC AACATCAGCC ATACACAAAC CCGCCTGCTC 
TCCATGGCCA AGCCTGTGTA CCATGCTATC ACCAAGCACT CGCCCAAGAA GCCTGTCATT 
GTCTTTGTGC CGTCTCGCAA GCAGACCCGC CTCACTGCCA TTGACATCCT CACCACCTGT 
GCAGCAGACA TCCAACGGCA GAGGTTCTTG CACTGCACCG AGAAGGATCT GATTCCGTAC 
CTGGAGAAGC TAAGTGACAG CACGCTCAAG GAAACGCTGC TAAATGGGGT GGGCTACCTG 
CATGAGGGGC TCAGCCCCAT GGAGCGACGC CTGGTGGAGC AGCTCTTCAG CTCAGGGGCT 
ATCCAGGTGG TGGTGGCTTC TCGGAGTCTC TGCTGGGGCA TGAACGTGGC TGCCCACCTG 
GTAATCATCA TGGATACCCA GTACTACAAT GGCAAGATCC ACGCCTATGT GGATTACCCC 
ATCTATGACG TGCTTCAGAT GGTGGGCCAC GCCAACCGCC CTTTGCAGGA CGATGAGGGG 
CGCTGTGTCA TCATGTGTCA GGGCTCCAAG AAGGATTTCT TCAAGAAGTT CTTATATGAG 
CCATTGCCAG TAGAATCTCA CCTGGACCAC TGTATGCATG ACCACTTCAA TGCTGAGATC 
GTCACCAAGA CCATTGAGAA CAAGCAGGAT GCTGTGGACT ACCTCACCTG GACCTTTCTG 
TACCGCCGCA TGACACAGAA CCCCAATTAC TACAACCTGC AGGGCATCTC CCATCGTCAC 
TTGTCGGACC ACTTGTCAGA GCTGGTGGAG CAGACCCTGA GTGACCTGGA GCAGTCCAAG 
TGCATCAGCA TCGAGGACGA GATGGACGTG GCGCCTCTGA ACCTAGGCAT GATCGCCGCC 
TACTATTACA TCAACTACAC CACCATTGAG CTCTTCAGCA TGTCCCTCAA TGCCAAGACC 
AAGGTGCGAG GGCTTATCGA GATCATCTCC AATGCAGCAG AGTATGAGAA CATTCCCATC 
CGGCACCATG AAGACAATCT CCTGAGGCAG TTGGCTCAGA AGGTCCCCCA CAAGCTGAAT 
AACCCTAAGT TCAATGATCC GCACGTCAAG ACCAACCTGC TCCTGCAGGC TCACTTGTCT 
CGCATGCAGC TGAGTGCTGA GTTGCAGTCA GATACGGAGG AAATCCTTAG TAAGGCAATC 
CGGCTCATCC AGGCCTGCGT GGATGTCCTT TCCAGCAATG GGTGGCTCAG CCCTGCTCTG 
GCAGCTATGG AACTGGCCCA GATGGTCACC CAAGCCATGT GGTCCAAGGA CTCATACCTG 
AAGCAGCTGC CACACTTCAC CTCTGAGCAT ATCAAACGTT GCACAGACAA GGGAGTGGAG 
AGTGTTTTCG ACATCATGGA GATGGAGGAT GAAGAACGGA ACGCGTTGCT TCAGCTGACT 
GACAGCCAGA TTGCAGATGT GGCTCGCTTT TGTAACCGCT ACCCTAATAT CGAACTATCT 
TATGAGGTGG TAGATAAGGA CAGCATCCGC AGTGGCGGGC CAGTTGTGGT GCTGGTGCAG 
CTGGAGCGAG AGGAGGAAGT CACAGGCCCT GTCATTGCGC CTCTCTTCCC GCAGAAACGT 
GAAGAGGGCT GGTGGGTGGT GATTGGAGAT GCCAAGTCCA ATAGCCTCAT CTCCATCAAG 
AGGCTGACCT TGCAGCAGAA GGCCAAGGTG AAGTTGGACT TTGTGGCCCC AGCCACTGGT 
GCCCACAACT ACACTCTGTA CTTCATGAGT GACGCTTACA TGGGATGTGA CCAGGAGTAC 
AAATTCAGCG TGGATGTGAA AGAAGCTGAG ACAGACAGTG ATTCAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRNP200 ( NM_014014.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_054733.2 Homo sapiens small nuclear ribonucleoprotein U5 subunit 200 (SNRNP200), mRNA.

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CloneID	OHu18366
Clone ID Related Accession (Same CDS sequence)	XM_017003603.1 , NM_014014.4 , NM_014014.5
Accession Version	NM_014014.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	U5 small nuclear ribonucleoprotein 200 kDa helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB067751.1, AY572488.1, AB018331.2 and AW190145.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_014014.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.187493.1, SRR1803611.60324.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000323853.10/ ENSP00000317123.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGATG TAACCGCCCG TAGTCTGCAA TACGAGTACA AGGCGAACTC GAATCTTGTG 
CTCCAAGCTG ACCGTTCTCT CATTGACCGG ACCCGCCGGG ATGAACCCAC AGGAGAGGTG 
CTGTCCCTTG TTGGGAAGCT GGAGGGCACC CGTATGGGAG ACAAGGCTCA ACGGACCAAA 
CCGCAGATGC AGGAGGAAAG AAGAGCCAAG CGAAGAAAGC GTGATGAGGA CCGGCATGAC 
ATCAACAAGA TGAAGGGTTA TACTCTGCTG TCGGAGGGCA TTGATGAGAT GGTGGGCATC 
ATCTACAAGC CCAAAACTAA AGAGACTCGG GAGACCTATG AGGTGCTACT CAGCTTCATC 
CAGGCTGCTC TTGGGGACCA GCCACGTGAT ATCCTTTGTG GGGCAGCTGA TGAAGTTCTA 
GCTGTTCTAA AGAATGAAAA GCTGCGGGAC AAGGAAAGGC GAAAGGAGAT TGACCTGCTG 
CTGGGTCAAA CAGATGATAC CAGATACCAT GTGCTAGTGA ACCTGGGCAA AAAGATCACA 
GACTATGGTG GAGATAAGGA AATCCAAAAT ATGGATGACA ACATTGATGA GACATACGGT 
GTGAATGTGC AGTTTGAGTC TGATGAGGAG GAAGGTGATG AAGACGTATA CGGGGAGGTT 
CGAGAAGAGG CATCTGATGA TGACATGGAA GGGGACGAGG CTGTCGTGCG CTGCACCCTC 
TCGGCTAATC TCGTAGCCTC AGGTGAACTG ATGAGTTCCA AGAAGAAGGA TTTGCACCCT 
CGGGATATTG ATGCATTTTG GCTGCAGCGG CAGCTCAGTC GTTTCTATGA TGATGCCATC 
GTGTCGCAGA AGAAGGCAGA TGAAGTATTG GAGATTTTGA AGACGGCCAG TGATGATCGG 
GAATGTGAAA ATCAGCTGGT TCTGCTGCTT GGTTTCAACA CCTTTGATTT CATTAAAGTG 
TTGCGGCAGC ACAGGATGAT GATTTTATAC TGTACCTTGC TGGCCAGTGC ACAAAGTGAA 
GCTGAAAAGG AAAGGATTAT GGGAAAGATG GAAGCTGACC CAGAGCTATC CAAGTTCCTC 
TACCAGCTTC ATGAAACCGA GAAGGAGGAT CTGATCCGAG AGGAAAGGTC CCGGAGAGAG 
CGAGTGCGTC AGTCTCGAAT GGACACAGAT CTGGAAACCA TGGATCTCGA CCAGGGTGGA 
GAGGCACTGG CTCCACGGCA GGTTCTGGAC TTGGAGGACC TGGTTTTTAC CCAAGGGAGC 
CACTTTATGG CCAATAAACG CTGTCAGCTT CCTGATGGAT CCTTCCGTCG CCAGCGTAAG 
GGCTATGAAG AGGTGCATGT GCCTGCTCTG AAGCCCAAGC CCTTTGGCTC AGAAGAACAA 
CTGCTTCCAG TGGAAAAGCT GCCAAAGTAT GCCCAGGCTG GGTTTGAGGG CTTCAAAACA 
CTGAATCGGA TCCAGAGTAA GCTCTACCGT GCTGCCCTTG AGACGGATGA GAATCTGCTG 
CTGTGTGCTC CTACTGGTGC TGGGAAGACC AACGTGGCCC TGATGTGCAT GCTCCGAGAG 
ATTGGGAAAC ACATAAACAT GGACGGCACC ATCAATGTGG ATGACTTCAA GATTATCTAC 
ATTGCCCCCA TGCGCTCCTT GGTGCAGGAG ATGGTGGGCA GCTTTGGAAA GCGCCTGGCC 
ACTTATGGCA TCACTGTTGC TGAACTGACT GGGGACCACC AGCTGTGCAA AGAAGAGATC 
AGTGCCACTC AGATCATCGT CTGCACCCCC GAGAAGTGGG ACATCATCAC CCGCAAGGGT 
GGTGAGCGCA CCTACACCCA GCTGGTGCGG CTCATCATTC TGGATGAGAT TCATCTTCTC 
CACGATGACA GAGGTCCTGT CTTAGAAGCT TTAGTGGCCA GGGCCATCCG AAACATTGAG 
ATGACCCAAG AGGATGTCCG ACTCATTGGT CTCAGTGCCA CCCTACCCAA CTATGAAGAT 
GTAGCCACCT TTCTACGTGT TGACCCTGCC AAGGGTCTCT TTTACTTTGA CAACAGCTTC 
CGTCCAGTGC CTCTGGAACA GACATATGTG GGTATCACAG AGAAAAAAGC TATCAAGCGT 
TTCCAGATCA TGAATGAAAT CGTCTATGAA AAAATCATGG AACATGCTGG AAAAAATCAG 
GTGCTGGTGT TTGTCCACTC CCGGAAGGAG ACTGGAAAGA CAGCCAGGGC CATCCGGGAC 
ATGTGCCTAG AAAAGGACAC TCTGGGTCTG TTTCTGAGGG AGGGCTCAGC CTCCACAGAA 
GTCCTGCGAA CAGAAGCTGA GCAGTGCAAG AACCTAGAGC TGAAGGATCT TCTGCCTTAT 
GGCTTTGCTA TTCATCACGC AGGCATGACC AGGGTTGACC GAACACTCGT GGAGGATCTT 
TTTGCTGATA AACATATTCA GGTTTTAGTT TCCACAGCAA CTCTAGCTTG GGGTGTGAAT 
CTCCCTGCAC ATACAGTCAT CATCAAAGGC ACCCAGGTGT ACAGTCCAGA GAAGGGGCGT 
TGGACAGAAC TGGGAGCACT GGACATTCTG CAGATGCTGG GACGTGCCGG AAGACCCCAG 
TATGACACCA AGGGTGAAGG CATACTCATC ACATCTCATG GGGAGCTACA GTACTACCTG 
TCCCTCCTCA ATCAACAACT TCCTATTGAA AGCCAGATGG TTTCAAAGCT TCCTGACATG 
CTCAATGCAG AAATCGTGCT AGGAAATGTC CAGAATGCCA AGGATGCGGT GAACTGGCTG 
GGCTATGCCT ACCTCTATAT CCGAATGCTG CGATCCCCAA CCCTCTATGG CATCTCTCAT 
GATGACCTCA AGGGAGATCC CCTGCTGGAC CAGCGCCGAC TAGATCTGGT TCATACAGCT 
GCCCTGATGC TGGACAAGAA CAATCTGGTC AAGTACGACA AGAAGACGGG CAACTTCCAG 
GTGACAGAAC TGGGCCGTAT AGCCAGCCAC TACTACATCA CCAATGATAC AGTGCAGACT 
TACAACCAGC TGCTGAAGCC CACCCTGAGT GAGATTGAGC TTTTCAGGGT CTTCTCATTG 
TCCTCTGAGT TCAAGAACAT CACAGTGAGA GAGGAGGAGA AGCTGGAGCT GCAGAAGTTG 
CTGGAGAGGG TGCCTATCCC TGTAAAGGAG AGCATTGAGG AACCCAGTGC TAAGATCAAC 
GTTCTTCTGC AAGCCTTCAT CTCACAGCTG AAATTGGAGG GCTTTGCACT GATGGCTGAC 
ATGGTGTATG TCACACAGTC GGCTGGCCGG TTGATGCGAG CGATATTTGA AATTGTCCTG 
AACCGAGGTT GGGCACAGCT TACAGACAAG ACCCTGAACC TCTGCAAGAT GATCGACAAA 
CGCATGTGGC AGTCCATGTG TCCTCTGCGC CAGTTCCGGA AACTCCCTGA GGAAGTAGTG 
AAGAAGATTG AGAAGAAGAA TTTCCCCTTT GAGCGTCTGT ACGACCTGAA TCATAATGAG 
ATTGGGGAGC TTATCCGCAT GCCAAAGATG GGGAAGACCA TCCACAAATA TGTCCATCTG 
TTTCCCAAGT TGGAGTTGTC AGTGCACCTG CAGCCTATCA CACGCTCCAC CCTGAAGGTG 
GAGCTGACCA TCACGCCAGA CTTCCAGTGG GATGAAAAGG TGCATGGTTC ATCCGAGGCT 
TTTTGGATTC TGGTGGAGGA TGTGGACAGC GAGGTGATTC TGCACCATGA GTATTTTCTC 
CTCAAGGCCA AGTACGCCCA GGACGAGCAC CTCATTACAT TCTTCGTGCC TGTCTTTGAA 
CCGCTGCCCC CTCAGTACTT CATCCGAGTG GTGTCTGACC GCTGGCTCTC TTGTGAGACC 
CAGCTGCCTG TCTCCTTCCG GCACCTGATC TTGCCGGAGA AGTACCCCCC TCCAACCGAA 
CTTTTGGACC TGCAGCCCTT GCCCGTGTCT GCTCTGAGAA ACAGTGCCTT TGAGAGTCTT 
TACCAAGATA AATTTCCTTT CTTCAATCCC ATCCAGACCC AGGTGTTTAA CACTGTATAC 
AACAGTGACG ACAACGTGTT TGTGGGGGCC CCCACGGGCA GCGGGAAGAC TATTTGTGCA 
GAGTTTGCCA TCCTGCGAAT GCTGCTGCAG AGCTCGGAGG GGCGCTGTGT GTACATCACC 
CCCATGGAGG CCCTGGCAGA GCAGGTATAC ATGGACTGGT ACGAGAAGTT CCAGGACAGG 
CTCAACAAGA AGGTGGTACT CCTGACAGGC GAGACCAGCA CAGACCTGAA GCTGCTGGGC 
AAAGGGAACA TTATCATCAG CACCCCTGAG AAGTGGGACA TACTTTCCCG GCGATGGAAG 
CAGCGCAAGA ACGTGCAGAA CATCAACCTC TTCGTGGTGG ATGAGGTCCA CCTTATCGGG 
GGCGAGAATG GGCCTGTCTT AGAAGTGATC TGCTCCCGAA TGCGCTACAT CTCCTCCCAG 
ATTGAGCGGC CCATTCGCAT TGTGGCACTC AGCTCTTCGC TCTCCAATGC CAAGGATGTG 
GCCCACTGGC TGGGCTGCAG TGCCACCTCC ACCTTCAACT TCCATCCCAA TGTGCGTCCC 
GTCCCCTTGG AGCTGCACAT CCAGGGCTTC AACATCAGCC ATACACAAAC CCGCCTGCTC 
TCCATGGCCA AGCCTGTGTA CCATGCTATC ACCAAGCACT CGCCCAAGAA GCCTGTCATT 
GTCTTTGTGC CGTCTCGCAA GCAGACCCGC CTCACTGCCA TTGACATCCT CACCACCTGT 
GCAGCAGACA TCCAACGGCA GAGGTTCTTG CACTGCACCG AGAAGGATCT GATTCCGTAC 
CTGGAGAAGC TAAGTGACAG CACGCTCAAG GAAACGCTGC TAAATGGGGT GGGCTACCTG 
CATGAGGGGC TCAGCCCCAT GGAGCGACGC CTGGTGGAGC AGCTCTTCAG CTCAGGGGCT 
ATCCAGGTGG TGGTGGCTTC TCGGAGTCTC TGCTGGGGCA TGAACGTGGC TGCCCACCTG 
GTAATCATCA TGGATACCCA GTACTACAAT GGCAAGATCC ACGCCTATGT GGATTACCCC 
ATCTATGACG TGCTTCAGAT GGTGGGCCAC GCCAACCGCC CTTTGCAGGA CGATGAGGGG 
CGCTGTGTCA TCATGTGTCA GGGCTCCAAG AAGGATTTCT TCAAGAAGTT CTTATATGAG 
CCATTGCCAG TAGAATCTCA CCTGGACCAC TGTATGCATG ACCACTTCAA TGCTGAGATC 
GTCACCAAGA CCATTGAGAA CAAGCAGGAT GCTGTGGACT ACCTCACCTG GACCTTTCTG 
TACCGCCGCA TGACACAGAA CCCCAATTAC TACAACCTGC AGGGCATCTC CCATCGTCAC 
TTGTCGGACC ACTTGTCAGA GCTGGTGGAG CAGACCCTGA GTGACCTGGA GCAGTCCAAG 
TGCATCAGCA TCGAGGACGA GATGGACGTG GCGCCTCTGA ACCTAGGCAT GATCGCCGCC 
TACTATTACA TCAACTACAC CACCATTGAG CTCTTCAGCA TGTCCCTCAA TGCCAAGACC 
AAGGTGCGAG GGCTTATCGA GATCATCTCC AATGCAGCAG AGTATGAGAA CATTCCCATC 
CGGCACCATG AAGACAATCT CCTGAGGCAG TTGGCTCAGA AGGTCCCCCA CAAGCTGAAT 
AACCCTAAGT TCAATGATCC GCACGTCAAG ACCAACCTGC TCCTGCAGGC TCACTTGTCT 
CGCATGCAGC TGAGTGCTGA GTTGCAGTCA GATACGGAGG AAATCCTTAG TAAGGCAATC 
CGGCTCATCC AGGCCTGCGT GGATGTCCTT TCCAGCAATG GGTGGCTCAG CCCTGCTCTG 
GCAGCTATGG AACTGGCCCA GATGGTCACC CAAGCCATGT GGTCCAAGGA CTCATACCTG 
AAGCAGCTGC CACACTTCAC CTCTGAGCAT ATCAAACGTT GCACAGACAA GGGAGTGGAG 
AGTGTTTTCG ACATCATGGA GATGGAGGAT GAAGAACGGA ACGCGTTGCT TCAGCTGACT 
GACAGCCAGA TTGCAGATGT GGCTCGCTTT TGTAACCGCT ACCCTAATAT CGAACTATCT 
TATGAGGTGG TAGATAAGGA CAGCATCCGC AGTGGCGGGC CAGTTGTGGT GCTGGTGCAG 
CTGGAGCGAG AGGAGGAAGT CACAGGCCCT GTCATTGCGC CTCTCTTCCC GCAGAAACGT 
GAAGAGGGCT GGTGGGTGGT GATTGGAGAT GCCAAGTCCA ATAGCCTCAT CTCCATCAAG 
AGGCTGACCT TGCAGCAGAA GGCCAAGGTG AAGTTGGACT TTGTGGCCCC AGCCACTGGT 
GCCCACAACT ACACTCTGTA CTTCATGAGT GACGCTTACA TGGGATGTGA CCAGGAGTAC 
AAATTCAGCG TGGATGTGAA AGAAGCTGAG ACAGACAGTG ATTCAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054733.2
CDS	110..6520
Translation	MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTKPQMQEERRAKRRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFIQAALGDQPRDILCGAADEVLAVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKITDYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTLSANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFLYQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD

Target ORF information:

RefSeq Version	NM_014014.5
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein U5 subunit 200 (SNRNP200), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014014.5

ORF Insert Sequence:

ATGGCGGATG TAACCGCCCG TAGTCTGCAA TACGAGTACA AGGCGAACTC GAATCTTGTG 
CTCCAAGCTG ACCGTTCTCT CATTGACCGG ACCCGCCGGG ATGAACCCAC AGGAGAGGTG 
CTGTCCCTTG TTGGGAAGCT GGAGGGCACC CGTATGGGAG ACAAGGCTCA ACGGACCAAA 
CCGCAGATGC AGGAGGAAAG AAGAGCCAAG CGAAGAAAGC GTGATGAGGA CCGGCATGAC 
ATCAACAAGA TGAAGGGTTA TACTCTGCTG TCGGAGGGCA TTGATGAGAT GGTGGGCATC 
ATCTACAAGC CCAAAACTAA AGAGACTCGG GAGACCTATG AGGTGCTACT CAGCTTCATC 
CAGGCTGCTC TTGGGGACCA GCCACGTGAT ATCCTTTGTG GGGCAGCTGA TGAAGTTCTA 
GCTGTTCTAA AGAATGAAAA GCTGCGGGAC AAGGAAAGGC GAAAGGAGAT TGACCTGCTG 
CTGGGTCAAA CAGATGATAC CAGATACCAT GTGCTAGTGA ACCTGGGCAA AAAGATCACA 
GACTATGGTG GAGATAAGGA AATCCAAAAT ATGGATGACA ACATTGATGA GACATACGGT 
GTGAATGTGC AGTTTGAGTC TGATGAGGAG GAAGGTGATG AAGACGTATA CGGGGAGGTT 
CGAGAAGAGG CATCTGATGA TGACATGGAA GGGGACGAGG CTGTCGTGCG CTGCACCCTC 
TCGGCTAATC TCGTAGCCTC AGGTGAACTG ATGAGTTCCA AGAAGAAGGA TTTGCACCCT 
CGGGATATTG ATGCATTTTG GCTGCAGCGG CAGCTCAGTC GTTTCTATGA TGATGCCATC 
GTGTCGCAGA AGAAGGCAGA TGAAGTATTG GAGATTTTGA AGACGGCCAG TGATGATCGG 
GAATGTGAAA ATCAGCTGGT TCTGCTGCTT GGTTTCAACA CCTTTGATTT CATTAAAGTG 
TTGCGGCAGC ACAGGATGAT GATTTTATAC TGTACCTTGC TGGCCAGTGC ACAAAGTGAA 
GCTGAAAAGG AAAGGATTAT GGGAAAGATG GAAGCTGACC CAGAGCTATC CAAGTTCCTC 
TACCAGCTTC ATGAAACCGA GAAGGAGGAT CTGATCCGAG AGGAAAGGTC CCGGAGAGAG 
CGAGTGCGTC AGTCTCGAAT GGACACAGAT CTGGAAACCA TGGATCTCGA CCAGGGTGGA 
GAGGCACTGG CTCCACGGCA GGTTCTGGAC TTGGAGGACC TGGTTTTTAC CCAAGGGAGC 
CACTTTATGG CCAATAAACG CTGTCAGCTT CCTGATGGAT CCTTCCGTCG CCAGCGTAAG 
GGCTATGAAG AGGTGCATGT GCCTGCTCTG AAGCCCAAGC CCTTTGGCTC AGAAGAACAA 
CTGCTTCCAG TGGAAAAGCT GCCAAAGTAT GCCCAGGCTG GGTTTGAGGG CTTCAAAACA 
CTGAATCGGA TCCAGAGTAA GCTCTACCGT GCTGCCCTTG AGACGGATGA GAATCTGCTG 
CTGTGTGCTC CTACTGGTGC TGGGAAGACC AACGTGGCCC TGATGTGCAT GCTCCGAGAG 
ATTGGGAAAC ACATAAACAT GGACGGCACC ATCAATGTGG ATGACTTCAA GATTATCTAC 
ATTGCCCCCA TGCGCTCCTT GGTGCAGGAG ATGGTGGGCA GCTTTGGAAA GCGCCTGGCC 
ACTTATGGCA TCACTGTTGC TGAACTGACT GGGGACCACC AGCTGTGCAA AGAAGAGATC 
AGTGCCACTC AGATCATCGT CTGCACCCCC GAGAAGTGGG ACATCATCAC CCGCAAGGGT 
GGTGAGCGCA CCTACACCCA GCTGGTGCGG CTCATCATTC TGGATGAGAT TCATCTTCTC 
CACGATGACA GAGGTCCTGT CTTAGAAGCT TTAGTGGCCA GGGCCATCCG AAACATTGAG 
ATGACCCAAG AGGATGTCCG ACTCATTGGT CTCAGTGCCA CCCTACCCAA CTATGAAGAT 
GTAGCCACCT TTCTACGTGT TGACCCTGCC AAGGGTCTCT TTTACTTTGA CAACAGCTTC 
CGTCCAGTGC CTCTGGAACA GACATATGTG GGTATCACAG AGAAAAAAGC TATCAAGCGT 
TTCCAGATCA TGAATGAAAT CGTCTATGAA AAAATCATGG AACATGCTGG AAAAAATCAG 
GTGCTGGTGT TTGTCCACTC CCGGAAGGAG ACTGGAAAGA CAGCCAGGGC CATCCGGGAC 
ATGTGCCTAG AAAAGGACAC TCTGGGTCTG TTTCTGAGGG AGGGCTCAGC CTCCACAGAA 
GTCCTGCGAA CAGAAGCTGA GCAGTGCAAG AACCTAGAGC TGAAGGATCT TCTGCCTTAT 
GGCTTTGCTA TTCATCACGC AGGCATGACC AGGGTTGACC GAACACTCGT GGAGGATCTT 
TTTGCTGATA AACATATTCA GGTTTTAGTT TCCACAGCAA CTCTAGCTTG GGGTGTGAAT 
CTCCCTGCAC ATACAGTCAT CATCAAAGGC ACCCAGGTGT ACAGTCCAGA GAAGGGGCGT 
TGGACAGAAC TGGGAGCACT GGACATTCTG CAGATGCTGG GACGTGCCGG AAGACCCCAG 
TATGACACCA AGGGTGAAGG CATACTCATC ACATCTCATG GGGAGCTACA GTACTACCTG 
TCCCTCCTCA ATCAACAACT TCCTATTGAA AGCCAGATGG TTTCAAAGCT TCCTGACATG 
CTCAATGCAG AAATCGTGCT AGGAAATGTC CAGAATGCCA AGGATGCGGT GAACTGGCTG 
GGCTATGCCT ACCTCTATAT CCGAATGCTG CGATCCCCAA CCCTCTATGG CATCTCTCAT 
GATGACCTCA AGGGAGATCC CCTGCTGGAC CAGCGCCGAC TAGATCTGGT TCATACAGCT 
GCCCTGATGC TGGACAAGAA CAATCTGGTC AAGTACGACA AGAAGACGGG CAACTTCCAG 
GTGACAGAAC TGGGCCGTAT AGCCAGCCAC TACTACATCA CCAATGATAC AGTGCAGACT 
TACAACCAGC TGCTGAAGCC CACCCTGAGT GAGATTGAGC TTTTCAGGGT CTTCTCATTG 
TCCTCTGAGT TCAAGAACAT CACAGTGAGA GAGGAGGAGA AGCTGGAGCT GCAGAAGTTG 
CTGGAGAGGG TGCCTATCCC TGTAAAGGAG AGCATTGAGG AACCCAGTGC TAAGATCAAC 
GTTCTTCTGC AAGCCTTCAT CTCACAGCTG AAATTGGAGG GCTTTGCACT GATGGCTGAC 
ATGGTGTATG TCACACAGTC GGCTGGCCGG TTGATGCGAG CGATATTTGA AATTGTCCTG 
AACCGAGGTT GGGCACAGCT TACAGACAAG ACCCTGAACC TCTGCAAGAT GATCGACAAA 
CGCATGTGGC AGTCCATGTG TCCTCTGCGC CAGTTCCGGA AACTCCCTGA GGAAGTAGTG 
AAGAAGATTG AGAAGAAGAA TTTCCCCTTT GAGCGTCTGT ACGACCTGAA TCATAATGAG 
ATTGGGGAGC TTATCCGCAT GCCAAAGATG GGGAAGACCA TCCACAAATA TGTCCATCTG 
TTTCCCAAGT TGGAGTTGTC AGTGCACCTG CAGCCTATCA CACGCTCCAC CCTGAAGGTG 
GAGCTGACCA TCACGCCAGA CTTCCAGTGG GATGAAAAGG TGCATGGTTC ATCCGAGGCT 
TTTTGGATTC TGGTGGAGGA TGTGGACAGC GAGGTGATTC TGCACCATGA GTATTTTCTC 
CTCAAGGCCA AGTACGCCCA GGACGAGCAC CTCATTACAT TCTTCGTGCC TGTCTTTGAA 
CCGCTGCCCC CTCAGTACTT CATCCGAGTG GTGTCTGACC GCTGGCTCTC TTGTGAGACC 
CAGCTGCCTG TCTCCTTCCG GCACCTGATC TTGCCGGAGA AGTACCCCCC TCCAACCGAA 
CTTTTGGACC TGCAGCCCTT GCCCGTGTCT GCTCTGAGAA ACAGTGCCTT TGAGAGTCTT 
TACCAAGATA AATTTCCTTT CTTCAATCCC ATCCAGACCC AGGTGTTTAA CACTGTATAC 
AACAGTGACG ACAACGTGTT TGTGGGGGCC CCCACGGGCA GCGGGAAGAC TATTTGTGCA 
GAGTTTGCCA TCCTGCGAAT GCTGCTGCAG AGCTCGGAGG GGCGCTGTGT GTACATCACC 
CCCATGGAGG CCCTGGCAGA GCAGGTATAC ATGGACTGGT ACGAGAAGTT CCAGGACAGG 
CTCAACAAGA AGGTGGTACT CCTGACAGGC GAGACCAGCA CAGACCTGAA GCTGCTGGGC 
AAAGGGAACA TTATCATCAG CACCCCTGAG AAGTGGGACA TACTTTCCCG GCGATGGAAG 
CAGCGCAAGA ACGTGCAGAA CATCAACCTC TTCGTGGTGG ATGAGGTCCA CCTTATCGGG 
GGCGAGAATG GGCCTGTCTT AGAAGTGATC TGCTCCCGAA TGCGCTACAT CTCCTCCCAG 
ATTGAGCGGC CCATTCGCAT TGTGGCACTC AGCTCTTCGC TCTCCAATGC CAAGGATGTG 
GCCCACTGGC TGGGCTGCAG TGCCACCTCC ACCTTCAACT TCCATCCCAA TGTGCGTCCC 
GTCCCCTTGG AGCTGCACAT CCAGGGCTTC AACATCAGCC ATACACAAAC CCGCCTGCTC 
TCCATGGCCA AGCCTGTGTA CCATGCTATC ACCAAGCACT CGCCCAAGAA GCCTGTCATT 
GTCTTTGTGC CGTCTCGCAA GCAGACCCGC CTCACTGCCA TTGACATCCT CACCACCTGT 
GCAGCAGACA TCCAACGGCA GAGGTTCTTG CACTGCACCG AGAAGGATCT GATTCCGTAC 
CTGGAGAAGC TAAGTGACAG CACGCTCAAG GAAACGCTGC TAAATGGGGT GGGCTACCTG 
CATGAGGGGC TCAGCCCCAT GGAGCGACGC CTGGTGGAGC AGCTCTTCAG CTCAGGGGCT 
ATCCAGGTGG TGGTGGCTTC TCGGAGTCTC TGCTGGGGCA TGAACGTGGC TGCCCACCTG 
GTAATCATCA TGGATACCCA GTACTACAAT GGCAAGATCC ACGCCTATGT GGATTACCCC 
ATCTATGACG TGCTTCAGAT GGTGGGCCAC GCCAACCGCC CTTTGCAGGA CGATGAGGGG 
CGCTGTGTCA TCATGTGTCA GGGCTCCAAG AAGGATTTCT TCAAGAAGTT CTTATATGAG 
CCATTGCCAG TAGAATCTCA CCTGGACCAC TGTATGCATG ACCACTTCAA TGCTGAGATC 
GTCACCAAGA CCATTGAGAA CAAGCAGGAT GCTGTGGACT ACCTCACCTG GACCTTTCTG 
TACCGCCGCA TGACACAGAA CCCCAATTAC TACAACCTGC AGGGCATCTC CCATCGTCAC 
TTGTCGGACC ACTTGTCAGA GCTGGTGGAG CAGACCCTGA GTGACCTGGA GCAGTCCAAG 
TGCATCAGCA TCGAGGACGA GATGGACGTG GCGCCTCTGA ACCTAGGCAT GATCGCCGCC 
TACTATTACA TCAACTACAC CACCATTGAG CTCTTCAGCA TGTCCCTCAA TGCCAAGACC 
AAGGTGCGAG GGCTTATCGA GATCATCTCC AATGCAGCAG AGTATGAGAA CATTCCCATC 
CGGCACCATG AAGACAATCT CCTGAGGCAG TTGGCTCAGA AGGTCCCCCA CAAGCTGAAT 
AACCCTAAGT TCAATGATCC GCACGTCAAG ACCAACCTGC TCCTGCAGGC TCACTTGTCT 
CGCATGCAGC TGAGTGCTGA GTTGCAGTCA GATACGGAGG AAATCCTTAG TAAGGCAATC 
CGGCTCATCC AGGCCTGCGT GGATGTCCTT TCCAGCAATG GGTGGCTCAG CCCTGCTCTG 
GCAGCTATGG AACTGGCCCA GATGGTCACC CAAGCCATGT GGTCCAAGGA CTCATACCTG 
AAGCAGCTGC CACACTTCAC CTCTGAGCAT ATCAAACGTT GCACAGACAA GGGAGTGGAG 
AGTGTTTTCG ACATCATGGA GATGGAGGAT GAAGAACGGA ACGCGTTGCT TCAGCTGACT 
GACAGCCAGA TTGCAGATGT GGCTCGCTTT TGTAACCGCT ACCCTAATAT CGAACTATCT 
TATGAGGTGG TAGATAAGGA CAGCATCCGC AGTGGCGGGC CAGTTGTGGT GCTGGTGCAG 
CTGGAGCGAG AGGAGGAAGT CACAGGCCCT GTCATTGCGC CTCTCTTCCC GCAGAAACGT 
GAAGAGGGCT GGTGGGTGGT GATTGGAGAT GCCAAGTCCA ATAGCCTCAT CTCCATCAAG 
AGGCTGACCT TGCAGCAGAA GGCCAAGGTG AAGTTGGACT TTGTGGCCCC AGCCACTGGT 
GCCCACAACT ACACTCTGTA CTTCATGAGT GACGCTTACA TGGGATGTGA CCAGGAGTAC 
AAATTCAGCG TGGATGTGAA AGAAGCTGAG ACAGACAGTG ATTCAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRNP200 ( XM_017003603.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859092.1 Homo sapiens small nuclear ribonucleoprotein U5 subunit 200 (SNRNP200), transcript variant X1, mRNA.

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CloneID	OHu18366
Clone ID Related Accession (Same CDS sequence)	XM_017003603.1 , NM_014014.4 , NM_014014.5
Accession Version	XM_017003603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	U5 small nuclear ribonucleoprotein 200 kDa helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG TAACCGCCCG TAGTCTGCAA TACGAGTACA AGGCGAACTC GAATCTTGTG 
CTCCAAGCTG ACCGTTCTCT CATTGACCGG ACCCGCCGGG ATGAACCCAC AGGAGAGGTG 
CTGTCCCTTG TTGGGAAGCT GGAGGGCACC CGTATGGGAG ACAAGGCTCA ACGGACCAAA 
CCGCAGATGC AGGAGGAAAG AAGAGCCAAG CGAAGAAAGC GTGATGAGGA CCGGCATGAC 
ATCAACAAGA TGAAGGGTTA TACTCTGCTG TCGGAGGGCA TTGATGAGAT GGTGGGCATC 
ATCTACAAGC CCAAAACTAA AGAGACTCGG GAGACCTATG AGGTGCTACT CAGCTTCATC 
CAGGCTGCTC TTGGGGACCA GCCACGTGAT ATCCTTTGTG GGGCAGCTGA TGAAGTTCTA 
GCTGTTCTAA AGAATGAAAA GCTGCGGGAC AAGGAAAGGC GAAAGGAGAT TGACCTGCTG 
CTGGGTCAAA CAGATGATAC CAGATACCAT GTGCTAGTGA ACCTGGGCAA AAAGATCACA 
GACTATGGTG GAGATAAGGA AATCCAAAAT ATGGATGACA ACATTGATGA GACATACGGT 
GTGAATGTGC AGTTTGAGTC TGATGAGGAG GAAGGTGATG AAGACGTATA CGGGGAGGTT 
CGAGAAGAGG CATCTGATGA TGACATGGAA GGGGACGAGG CTGTCGTGCG CTGCACCCTC 
TCGGCTAATC TCGTAGCCTC AGGTGAACTG ATGAGTTCCA AGAAGAAGGA TTTGCACCCT 
CGGGATATTG ATGCATTTTG GCTGCAGCGG CAGCTCAGTC GTTTCTATGA TGATGCCATC 
GTGTCGCAGA AGAAGGCAGA TGAAGTATTG GAGATTTTGA AGACGGCCAG TGATGATCGG 
GAATGTGAAA ATCAGCTGGT TCTGCTGCTT GGTTTCAACA CCTTTGATTT CATTAAAGTG 
TTGCGGCAGC ACAGGATGAT GATTTTATAC TGTACCTTGC TGGCCAGTGC ACAAAGTGAA 
GCTGAAAAGG AAAGGATTAT GGGAAAGATG GAAGCTGACC CAGAGCTATC CAAGTTCCTC 
TACCAGCTTC ATGAAACCGA GAAGGAGGAT CTGATCCGAG AGGAAAGGTC CCGGAGAGAG 
CGAGTGCGTC AGTCTCGAAT GGACACAGAT CTGGAAACCA TGGATCTCGA CCAGGGTGGA 
GAGGCACTGG CTCCACGGCA GGTTCTGGAC TTGGAGGACC TGGTTTTTAC CCAAGGGAGC 
CACTTTATGG CCAATAAACG CTGTCAGCTT CCTGATGGAT CCTTCCGTCG CCAGCGTAAG 
GGCTATGAAG AGGTGCATGT GCCTGCTCTG AAGCCCAAGC CCTTTGGCTC AGAAGAACAA 
CTGCTTCCAG TGGAAAAGCT GCCAAAGTAT GCCCAGGCTG GGTTTGAGGG CTTCAAAACA 
CTGAATCGGA TCCAGAGTAA GCTCTACCGT GCTGCCCTTG AGACGGATGA GAATCTGCTG 
CTGTGTGCTC CTACTGGTGC TGGGAAGACC AACGTGGCCC TGATGTGCAT GCTCCGAGAG 
ATTGGGAAAC ACATAAACAT GGACGGCACC ATCAATGTGG ATGACTTCAA GATTATCTAC 
ATTGCCCCCA TGCGCTCCTT GGTGCAGGAG ATGGTGGGCA GCTTTGGAAA GCGCCTGGCC 
ACTTATGGCA TCACTGTTGC TGAACTGACT GGGGACCACC AGCTGTGCAA AGAAGAGATC 
AGTGCCACTC AGATCATCGT CTGCACCCCC GAGAAGTGGG ACATCATCAC CCGCAAGGGT 
GGTGAGCGCA CCTACACCCA GCTGGTGCGG CTCATCATTC TGGATGAGAT TCATCTTCTC 
CACGATGACA GAGGTCCTGT CTTAGAAGCT TTAGTGGCCA GGGCCATCCG AAACATTGAG 
ATGACCCAAG AGGATGTCCG ACTCATTGGT CTCAGTGCCA CCCTACCCAA CTATGAAGAT 
GTAGCCACCT TTCTACGTGT TGACCCTGCC AAGGGTCTCT TTTACTTTGA CAACAGCTTC 
CGTCCAGTGC CTCTGGAACA GACATATGTG GGTATCACAG AGAAAAAAGC TATCAAGCGT 
TTCCAGATCA TGAATGAAAT CGTCTATGAA AAAATCATGG AACATGCTGG AAAAAATCAG 
GTGCTGGTGT TTGTCCACTC CCGGAAGGAG ACTGGAAAGA CAGCCAGGGC CATCCGGGAC 
ATGTGCCTAG AAAAGGACAC TCTGGGTCTG TTTCTGAGGG AGGGCTCAGC CTCCACAGAA 
GTCCTGCGAA CAGAAGCTGA GCAGTGCAAG AACCTAGAGC TGAAGGATCT TCTGCCTTAT 
GGCTTTGCTA TTCATCACGC AGGCATGACC AGGGTTGACC GAACACTCGT GGAGGATCTT 
TTTGCTGATA AACATATTCA GGTTTTAGTT TCCACAGCAA CTCTAGCTTG GGGTGTGAAT 
CTCCCTGCAC ATACAGTCAT CATCAAAGGC ACCCAGGTGT ACAGTCCAGA GAAGGGGCGT 
TGGACAGAAC TGGGAGCACT GGACATTCTG CAGATGCTGG GACGTGCCGG AAGACCCCAG 
TATGACACCA AGGGTGAAGG CATACTCATC ACATCTCATG GGGAGCTACA GTACTACCTG 
TCCCTCCTCA ATCAACAACT TCCTATTGAA AGCCAGATGG TTTCAAAGCT TCCTGACATG 
CTCAATGCAG AAATCGTGCT AGGAAATGTC CAGAATGCCA AGGATGCGGT GAACTGGCTG 
GGCTATGCCT ACCTCTATAT CCGAATGCTG CGATCCCCAA CCCTCTATGG CATCTCTCAT 
GATGACCTCA AGGGAGATCC CCTGCTGGAC CAGCGCCGAC TAGATCTGGT TCATACAGCT 
GCCCTGATGC TGGACAAGAA CAATCTGGTC AAGTACGACA AGAAGACGGG CAACTTCCAG 
GTGACAGAAC TGGGCCGTAT AGCCAGCCAC TACTACATCA CCAATGATAC AGTGCAGACT 
TACAACCAGC TGCTGAAGCC CACCCTGAGT GAGATTGAGC TTTTCAGGGT CTTCTCATTG 
TCCTCTGAGT TCAAGAACAT CACAGTGAGA GAGGAGGAGA AGCTGGAGCT GCAGAAGTTG 
CTGGAGAGGG TGCCTATCCC TGTAAAGGAG AGCATTGAGG AACCCAGTGC TAAGATCAAC 
GTTCTTCTGC AAGCCTTCAT CTCACAGCTG AAATTGGAGG GCTTTGCACT GATGGCTGAC 
ATGGTGTATG TCACACAGTC GGCTGGCCGG TTGATGCGAG CGATATTTGA AATTGTCCTG 
AACCGAGGTT GGGCACAGCT TACAGACAAG ACCCTGAACC TCTGCAAGAT GATCGACAAA 
CGCATGTGGC AGTCCATGTG TCCTCTGCGC CAGTTCCGGA AACTCCCTGA GGAAGTAGTG 
AAGAAGATTG AGAAGAAGAA TTTCCCCTTT GAGCGTCTGT ACGACCTGAA TCATAATGAG 
ATTGGGGAGC TTATCCGCAT GCCAAAGATG GGGAAGACCA TCCACAAATA TGTCCATCTG 
TTTCCCAAGT TGGAGTTGTC AGTGCACCTG CAGCCTATCA CACGCTCCAC CCTGAAGGTG 
GAGCTGACCA TCACGCCAGA CTTCCAGTGG GATGAAAAGG TGCATGGTTC ATCCGAGGCT 
TTTTGGATTC TGGTGGAGGA TGTGGACAGC GAGGTGATTC TGCACCATGA GTATTTTCTC 
CTCAAGGCCA AGTACGCCCA GGACGAGCAC CTCATTACAT TCTTCGTGCC TGTCTTTGAA 
CCGCTGCCCC CTCAGTACTT CATCCGAGTG GTGTCTGACC GCTGGCTCTC TTGTGAGACC 
CAGCTGCCTG TCTCCTTCCG GCACCTGATC TTGCCGGAGA AGTACCCCCC TCCAACCGAA 
CTTTTGGACC TGCAGCCCTT GCCCGTGTCT GCTCTGAGAA ACAGTGCCTT TGAGAGTCTT 
TACCAAGATA AATTTCCTTT CTTCAATCCC ATCCAGACCC AGGTGTTTAA CACTGTATAC 
AACAGTGACG ACAACGTGTT TGTGGGGGCC CCCACGGGCA GCGGGAAGAC TATTTGTGCA 
GAGTTTGCCA TCCTGCGAAT GCTGCTGCAG AGCTCGGAGG GGCGCTGTGT GTACATCACC 
CCCATGGAGG CCCTGGCAGA GCAGGTATAC ATGGACTGGT ACGAGAAGTT CCAGGACAGG 
CTCAACAAGA AGGTGGTACT CCTGACAGGC GAGACCAGCA CAGACCTGAA GCTGCTGGGC 
AAAGGGAACA TTATCATCAG CACCCCTGAG AAGTGGGACA TACTTTCCCG GCGATGGAAG 
CAGCGCAAGA ACGTGCAGAA CATCAACCTC TTCGTGGTGG ATGAGGTCCA CCTTATCGGG 
GGCGAGAATG GGCCTGTCTT AGAAGTGATC TGCTCCCGAA TGCGCTACAT CTCCTCCCAG 
ATTGAGCGGC CCATTCGCAT TGTGGCACTC AGCTCTTCGC TCTCCAATGC CAAGGATGTG 
GCCCACTGGC TGGGCTGCAG TGCCACCTCC ACCTTCAACT TCCATCCCAA TGTGCGTCCC 
GTCCCCTTGG AGCTGCACAT CCAGGGCTTC AACATCAGCC ATACACAAAC CCGCCTGCTC 
TCCATGGCCA AGCCTGTGTA CCATGCTATC ACCAAGCACT CGCCCAAGAA GCCTGTCATT 
GTCTTTGTGC CGTCTCGCAA GCAGACCCGC CTCACTGCCA TTGACATCCT CACCACCTGT 
GCAGCAGACA TCCAACGGCA GAGGTTCTTG CACTGCACCG AGAAGGATCT GATTCCGTAC 
CTGGAGAAGC TAAGTGACAG CACGCTCAAG GAAACGCTGC TAAATGGGGT GGGCTACCTG 
CATGAGGGGC TCAGCCCCAT GGAGCGACGC CTGGTGGAGC AGCTCTTCAG CTCAGGGGCT 
ATCCAGGTGG TGGTGGCTTC TCGGAGTCTC TGCTGGGGCA TGAACGTGGC TGCCCACCTG 
GTAATCATCA TGGATACCCA GTACTACAAT GGCAAGATCC ACGCCTATGT GGATTACCCC 
ATCTATGACG TGCTTCAGAT GGTGGGCCAC GCCAACCGCC CTTTGCAGGA CGATGAGGGG 
CGCTGTGTCA TCATGTGTCA GGGCTCCAAG AAGGATTTCT TCAAGAAGTT CTTATATGAG 
CCATTGCCAG TAGAATCTCA CCTGGACCAC TGTATGCATG ACCACTTCAA TGCTGAGATC 
GTCACCAAGA CCATTGAGAA CAAGCAGGAT GCTGTGGACT ACCTCACCTG GACCTTTCTG 
TACCGCCGCA TGACACAGAA CCCCAATTAC TACAACCTGC AGGGCATCTC CCATCGTCAC 
TTGTCGGACC ACTTGTCAGA GCTGGTGGAG CAGACCCTGA GTGACCTGGA GCAGTCCAAG 
TGCATCAGCA TCGAGGACGA GATGGACGTG GCGCCTCTGA ACCTAGGCAT GATCGCCGCC 
TACTATTACA TCAACTACAC CACCATTGAG CTCTTCAGCA TGTCCCTCAA TGCCAAGACC 
AAGGTGCGAG GGCTTATCGA GATCATCTCC AATGCAGCAG AGTATGAGAA CATTCCCATC 
CGGCACCATG AAGACAATCT CCTGAGGCAG TTGGCTCAGA AGGTCCCCCA CAAGCTGAAT 
AACCCTAAGT TCAATGATCC GCACGTCAAG ACCAACCTGC TCCTGCAGGC TCACTTGTCT 
CGCATGCAGC TGAGTGCTGA GTTGCAGTCA GATACGGAGG AAATCCTTAG TAAGGCAATC 
CGGCTCATCC AGGCCTGCGT GGATGTCCTT TCCAGCAATG GGTGGCTCAG CCCTGCTCTG 
GCAGCTATGG AACTGGCCCA GATGGTCACC CAAGCCATGT GGTCCAAGGA CTCATACCTG 
AAGCAGCTGC CACACTTCAC CTCTGAGCAT ATCAAACGTT GCACAGACAA GGGAGTGGAG 
AGTGTTTTCG ACATCATGGA GATGGAGGAT GAAGAACGGA ACGCGTTGCT TCAGCTGACT 
GACAGCCAGA TTGCAGATGT GGCTCGCTTT TGTAACCGCT ACCCTAATAT CGAACTATCT 
TATGAGGTGG TAGATAAGGA CAGCATCCGC AGTGGCGGGC CAGTTGTGGT GCTGGTGCAG 
CTGGAGCGAG AGGAGGAAGT CACAGGCCCT GTCATTGCGC CTCTCTTCCC GCAGAAACGT 
GAAGAGGGCT GGTGGGTGGT GATTGGAGAT GCCAAGTCCA ATAGCCTCAT CTCCATCAAG 
AGGCTGACCT TGCAGCAGAA GGCCAAGGTG AAGTTGGACT TTGTGGCCCC AGCCACTGGT 
GCCCACAACT ACACTCTGTA CTTCATGAGT GACGCTTACA TGGGATGTGA CCAGGAGTAC 
AAATTCAGCG TGGATGTGAA AGAAGCTGAG ACAGACAGTG ATTCAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859092.1
CDS	963..7373
Translation	MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTKPQMQEERRAKRRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFIQAALGDQPRDILCGAADEVLAVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKITDYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTLSANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFLYQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD

Target ORF information:

RefSeq Version	XM_017003603.1
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein U5 subunit 200 (SNRNP200), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003603.1

ORF Insert Sequence:

ATGGCGGATG TAACCGCCCG TAGTCTGCAA TACGAGTACA AGGCGAACTC GAATCTTGTG 
CTCCAAGCTG ACCGTTCTCT CATTGACCGG ACCCGCCGGG ATGAACCCAC AGGAGAGGTG 
CTGTCCCTTG TTGGGAAGCT GGAGGGCACC CGTATGGGAG ACAAGGCTCA ACGGACCAAA 
CCGCAGATGC AGGAGGAAAG AAGAGCCAAG CGAAGAAAGC GTGATGAGGA CCGGCATGAC 
ATCAACAAGA TGAAGGGTTA TACTCTGCTG TCGGAGGGCA TTGATGAGAT GGTGGGCATC 
ATCTACAAGC CCAAAACTAA AGAGACTCGG GAGACCTATG AGGTGCTACT CAGCTTCATC 
CAGGCTGCTC TTGGGGACCA GCCACGTGAT ATCCTTTGTG GGGCAGCTGA TGAAGTTCTA 
GCTGTTCTAA AGAATGAAAA GCTGCGGGAC AAGGAAAGGC GAAAGGAGAT TGACCTGCTG 
CTGGGTCAAA CAGATGATAC CAGATACCAT GTGCTAGTGA ACCTGGGCAA AAAGATCACA 
GACTATGGTG GAGATAAGGA AATCCAAAAT ATGGATGACA ACATTGATGA GACATACGGT 
GTGAATGTGC AGTTTGAGTC TGATGAGGAG GAAGGTGATG AAGACGTATA CGGGGAGGTT 
CGAGAAGAGG CATCTGATGA TGACATGGAA GGGGACGAGG CTGTCGTGCG CTGCACCCTC 
TCGGCTAATC TCGTAGCCTC AGGTGAACTG ATGAGTTCCA AGAAGAAGGA TTTGCACCCT 
CGGGATATTG ATGCATTTTG GCTGCAGCGG CAGCTCAGTC GTTTCTATGA TGATGCCATC 
GTGTCGCAGA AGAAGGCAGA TGAAGTATTG GAGATTTTGA AGACGGCCAG TGATGATCGG 
GAATGTGAAA ATCAGCTGGT TCTGCTGCTT GGTTTCAACA CCTTTGATTT CATTAAAGTG 
TTGCGGCAGC ACAGGATGAT GATTTTATAC TGTACCTTGC TGGCCAGTGC ACAAAGTGAA 
GCTGAAAAGG AAAGGATTAT GGGAAAGATG GAAGCTGACC CAGAGCTATC CAAGTTCCTC 
TACCAGCTTC ATGAAACCGA GAAGGAGGAT CTGATCCGAG AGGAAAGGTC CCGGAGAGAG 
CGAGTGCGTC AGTCTCGAAT GGACACAGAT CTGGAAACCA TGGATCTCGA CCAGGGTGGA 
GAGGCACTGG CTCCACGGCA GGTTCTGGAC TTGGAGGACC TGGTTTTTAC CCAAGGGAGC 
CACTTTATGG CCAATAAACG CTGTCAGCTT CCTGATGGAT CCTTCCGTCG CCAGCGTAAG 
GGCTATGAAG AGGTGCATGT GCCTGCTCTG AAGCCCAAGC CCTTTGGCTC AGAAGAACAA 
CTGCTTCCAG TGGAAAAGCT GCCAAAGTAT GCCCAGGCTG GGTTTGAGGG CTTCAAAACA 
CTGAATCGGA TCCAGAGTAA GCTCTACCGT GCTGCCCTTG AGACGGATGA GAATCTGCTG 
CTGTGTGCTC CTACTGGTGC TGGGAAGACC AACGTGGCCC TGATGTGCAT GCTCCGAGAG 
ATTGGGAAAC ACATAAACAT GGACGGCACC ATCAATGTGG ATGACTTCAA GATTATCTAC 
ATTGCCCCCA TGCGCTCCTT GGTGCAGGAG ATGGTGGGCA GCTTTGGAAA GCGCCTGGCC 
ACTTATGGCA TCACTGTTGC TGAACTGACT GGGGACCACC AGCTGTGCAA AGAAGAGATC 
AGTGCCACTC AGATCATCGT CTGCACCCCC GAGAAGTGGG ACATCATCAC CCGCAAGGGT 
GGTGAGCGCA CCTACACCCA GCTGGTGCGG CTCATCATTC TGGATGAGAT TCATCTTCTC 
CACGATGACA GAGGTCCTGT CTTAGAAGCT TTAGTGGCCA GGGCCATCCG AAACATTGAG 
ATGACCCAAG AGGATGTCCG ACTCATTGGT CTCAGTGCCA CCCTACCCAA CTATGAAGAT 
GTAGCCACCT TTCTACGTGT TGACCCTGCC AAGGGTCTCT TTTACTTTGA CAACAGCTTC 
CGTCCAGTGC CTCTGGAACA GACATATGTG GGTATCACAG AGAAAAAAGC TATCAAGCGT 
TTCCAGATCA TGAATGAAAT CGTCTATGAA AAAATCATGG AACATGCTGG AAAAAATCAG 
GTGCTGGTGT TTGTCCACTC CCGGAAGGAG ACTGGAAAGA CAGCCAGGGC CATCCGGGAC 
ATGTGCCTAG AAAAGGACAC TCTGGGTCTG TTTCTGAGGG AGGGCTCAGC CTCCACAGAA 
GTCCTGCGAA CAGAAGCTGA GCAGTGCAAG AACCTAGAGC TGAAGGATCT TCTGCCTTAT 
GGCTTTGCTA TTCATCACGC AGGCATGACC AGGGTTGACC GAACACTCGT GGAGGATCTT 
TTTGCTGATA AACATATTCA GGTTTTAGTT TCCACAGCAA CTCTAGCTTG GGGTGTGAAT 
CTCCCTGCAC ATACAGTCAT CATCAAAGGC ACCCAGGTGT ACAGTCCAGA GAAGGGGCGT 
TGGACAGAAC TGGGAGCACT GGACATTCTG CAGATGCTGG GACGTGCCGG AAGACCCCAG 
TATGACACCA AGGGTGAAGG CATACTCATC ACATCTCATG GGGAGCTACA GTACTACCTG 
TCCCTCCTCA ATCAACAACT TCCTATTGAA AGCCAGATGG TTTCAAAGCT TCCTGACATG 
CTCAATGCAG AAATCGTGCT AGGAAATGTC CAGAATGCCA AGGATGCGGT GAACTGGCTG 
GGCTATGCCT ACCTCTATAT CCGAATGCTG CGATCCCCAA CCCTCTATGG CATCTCTCAT 
GATGACCTCA AGGGAGATCC CCTGCTGGAC CAGCGCCGAC TAGATCTGGT TCATACAGCT 
GCCCTGATGC TGGACAAGAA CAATCTGGTC AAGTACGACA AGAAGACGGG CAACTTCCAG 
GTGACAGAAC TGGGCCGTAT AGCCAGCCAC TACTACATCA CCAATGATAC AGTGCAGACT 
TACAACCAGC TGCTGAAGCC CACCCTGAGT GAGATTGAGC TTTTCAGGGT CTTCTCATTG 
TCCTCTGAGT TCAAGAACAT CACAGTGAGA GAGGAGGAGA AGCTGGAGCT GCAGAAGTTG 
CTGGAGAGGG TGCCTATCCC TGTAAAGGAG AGCATTGAGG AACCCAGTGC TAAGATCAAC 
GTTCTTCTGC AAGCCTTCAT CTCACAGCTG AAATTGGAGG GCTTTGCACT GATGGCTGAC 
ATGGTGTATG TCACACAGTC GGCTGGCCGG TTGATGCGAG CGATATTTGA AATTGTCCTG 
AACCGAGGTT GGGCACAGCT TACAGACAAG ACCCTGAACC TCTGCAAGAT GATCGACAAA 
CGCATGTGGC AGTCCATGTG TCCTCTGCGC CAGTTCCGGA AACTCCCTGA GGAAGTAGTG 
AAGAAGATTG AGAAGAAGAA TTTCCCCTTT GAGCGTCTGT ACGACCTGAA TCATAATGAG 
ATTGGGGAGC TTATCCGCAT GCCAAAGATG GGGAAGACCA TCCACAAATA TGTCCATCTG 
TTTCCCAAGT TGGAGTTGTC AGTGCACCTG CAGCCTATCA CACGCTCCAC CCTGAAGGTG 
GAGCTGACCA TCACGCCAGA CTTCCAGTGG GATGAAAAGG TGCATGGTTC ATCCGAGGCT 
TTTTGGATTC TGGTGGAGGA TGTGGACAGC GAGGTGATTC TGCACCATGA GTATTTTCTC 
CTCAAGGCCA AGTACGCCCA GGACGAGCAC CTCATTACAT TCTTCGTGCC TGTCTTTGAA 
CCGCTGCCCC CTCAGTACTT CATCCGAGTG GTGTCTGACC GCTGGCTCTC TTGTGAGACC 
CAGCTGCCTG TCTCCTTCCG GCACCTGATC TTGCCGGAGA AGTACCCCCC TCCAACCGAA 
CTTTTGGACC TGCAGCCCTT GCCCGTGTCT GCTCTGAGAA ACAGTGCCTT TGAGAGTCTT 
TACCAAGATA AATTTCCTTT CTTCAATCCC ATCCAGACCC AGGTGTTTAA CACTGTATAC 
AACAGTGACG ACAACGTGTT TGTGGGGGCC CCCACGGGCA GCGGGAAGAC TATTTGTGCA 
GAGTTTGCCA TCCTGCGAAT GCTGCTGCAG AGCTCGGAGG GGCGCTGTGT GTACATCACC 
CCCATGGAGG CCCTGGCAGA GCAGGTATAC ATGGACTGGT ACGAGAAGTT CCAGGACAGG 
CTCAACAAGA AGGTGGTACT CCTGACAGGC GAGACCAGCA CAGACCTGAA GCTGCTGGGC 
AAAGGGAACA TTATCATCAG CACCCCTGAG AAGTGGGACA TACTTTCCCG GCGATGGAAG 
CAGCGCAAGA ACGTGCAGAA CATCAACCTC TTCGTGGTGG ATGAGGTCCA CCTTATCGGG 
GGCGAGAATG GGCCTGTCTT AGAAGTGATC TGCTCCCGAA TGCGCTACAT CTCCTCCCAG 
ATTGAGCGGC CCATTCGCAT TGTGGCACTC AGCTCTTCGC TCTCCAATGC CAAGGATGTG 
GCCCACTGGC TGGGCTGCAG TGCCACCTCC ACCTTCAACT TCCATCCCAA TGTGCGTCCC 
GTCCCCTTGG AGCTGCACAT CCAGGGCTTC AACATCAGCC ATACACAAAC CCGCCTGCTC 
TCCATGGCCA AGCCTGTGTA CCATGCTATC ACCAAGCACT CGCCCAAGAA GCCTGTCATT 
GTCTTTGTGC CGTCTCGCAA GCAGACCCGC CTCACTGCCA TTGACATCCT CACCACCTGT 
GCAGCAGACA TCCAACGGCA GAGGTTCTTG CACTGCACCG AGAAGGATCT GATTCCGTAC 
CTGGAGAAGC TAAGTGACAG CACGCTCAAG GAAACGCTGC TAAATGGGGT GGGCTACCTG 
CATGAGGGGC TCAGCCCCAT GGAGCGACGC CTGGTGGAGC AGCTCTTCAG CTCAGGGGCT 
ATCCAGGTGG TGGTGGCTTC TCGGAGTCTC TGCTGGGGCA TGAACGTGGC TGCCCACCTG 
GTAATCATCA TGGATACCCA GTACTACAAT GGCAAGATCC ACGCCTATGT GGATTACCCC 
ATCTATGACG TGCTTCAGAT GGTGGGCCAC GCCAACCGCC CTTTGCAGGA CGATGAGGGG 
CGCTGTGTCA TCATGTGTCA GGGCTCCAAG AAGGATTTCT TCAAGAAGTT CTTATATGAG 
CCATTGCCAG TAGAATCTCA CCTGGACCAC TGTATGCATG ACCACTTCAA TGCTGAGATC 
GTCACCAAGA CCATTGAGAA CAAGCAGGAT GCTGTGGACT ACCTCACCTG GACCTTTCTG 
TACCGCCGCA TGACACAGAA CCCCAATTAC TACAACCTGC AGGGCATCTC CCATCGTCAC 
TTGTCGGACC ACTTGTCAGA GCTGGTGGAG CAGACCCTGA GTGACCTGGA GCAGTCCAAG 
TGCATCAGCA TCGAGGACGA GATGGACGTG GCGCCTCTGA ACCTAGGCAT GATCGCCGCC 
TACTATTACA TCAACTACAC CACCATTGAG CTCTTCAGCA TGTCCCTCAA TGCCAAGACC 
AAGGTGCGAG GGCTTATCGA GATCATCTCC AATGCAGCAG AGTATGAGAA CATTCCCATC 
CGGCACCATG AAGACAATCT CCTGAGGCAG TTGGCTCAGA AGGTCCCCCA CAAGCTGAAT 
AACCCTAAGT TCAATGATCC GCACGTCAAG ACCAACCTGC TCCTGCAGGC TCACTTGTCT 
CGCATGCAGC TGAGTGCTGA GTTGCAGTCA GATACGGAGG AAATCCTTAG TAAGGCAATC 
CGGCTCATCC AGGCCTGCGT GGATGTCCTT TCCAGCAATG GGTGGCTCAG CCCTGCTCTG 
GCAGCTATGG AACTGGCCCA GATGGTCACC CAAGCCATGT GGTCCAAGGA CTCATACCTG 
AAGCAGCTGC CACACTTCAC CTCTGAGCAT ATCAAACGTT GCACAGACAA GGGAGTGGAG 
AGTGTTTTCG ACATCATGGA GATGGAGGAT GAAGAACGGA ACGCGTTGCT TCAGCTGACT 
GACAGCCAGA TTGCAGATGT GGCTCGCTTT TGTAACCGCT ACCCTAATAT CGAACTATCT 
TATGAGGTGG TAGATAAGGA CAGCATCCGC AGTGGCGGGC CAGTTGTGGT GCTGGTGCAG 
CTGGAGCGAG AGGAGGAAGT CACAGGCCCT GTCATTGCGC CTCTCTTCCC GCAGAAACGT 
GAAGAGGGCT GGTGGGTGGT GATTGGAGAT GCCAAGTCCA ATAGCCTCAT CTCCATCAAG 
AGGCTGACCT TGCAGCAGAA GGCCAAGGTG AAGTTGGACT TTGTGGCCCC AGCCACTGGT 
GCCCACAACT ACACTCTGTA CTTCATGAGT GACGCTTACA TGGGATGTGA CCAGGAGTAC 
AAATTCAGCG TGGATGTGAA AGAAGCTGAG ACAGACAGTG ATTCAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of a human catalytic step I spliceosome.
Science (New York, N.Y.)(2018 Jan)
Zhan X,Yan C,Zhang X,Lei J,Shi Y

Variability in clinical phenotypes of PRPF8-linked autosomal dominant retinitis pigmentosa correlates with differential PRPF8/SNRNP200 interactions.
Ophthalmic genetics39(1)80-86(2018 Jan-Feb)
Escher P,Passarin O,Munier FL,Tran VH,Vaclavik V

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オンライン注文

SNRNP200 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

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