Snrnp200 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Snrnp200
Entrez Gene ID	320632
Full Name	small nuclear ribonucleoprotein 200 (U5)
Synonyms	A330064G03Rik,Ascc3l1,BC011390,HELIC2,U5-200-KD,U5-200KD
General protein information	Preferred Names small nuclear ribonucleoprotein 200 (U5) Names U5 small nuclear ribonucleoprotein 200 kDa helicase BRR2 homolog U5 snRNP-specific 200 kDa protein U5 snRNP-specific protein, 200 kDa (DEXH RNA helicase family) activating signal cointegrator 1 complex subunit 3-like 1 small nuclear ribonucleoprotein 200kDa (U5)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 F1\|2 61.84 cM
Summary	On February 19, 2002, this locus was switched from human to mouse. The source accession, Z70200.1, is almost identical to the mouse BAC clone AC074224, and it matches the mouse cDNA accession BC011390 as well. The human gene is LocusID 23020. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017319022.1	XP_017174511.1	U5 small nuclear ribonucleoprotein 200 kDa helicase isoform X1
NM_177214.4	NP_796188.2	U5 small nuclear ribonucleoprotein 200 kDa helicase
NM_177214.5	NP_796188.2	U5 small nuclear ribonucleoprotein 200 kDa helicase

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	brr2	NP_593346.1
Canis lupus familiaris (dog)	SNRNP200	XP_532949.2
Bos taurus (cattle)	SNRNP200	NP_001193092.1
Caenorhabditis elegans (roundworm)	snrp-200	NP_496710.1
Kluyveromyces lactis	KLLA0C17798g	XP_452995.1
Arabidopsis thaliana (thale cress)	AT2G42270	NP_181756.1
Pan troglodytes (chimpanzee)	SNRNP200	XP_001147672.1
Saccharomyces cerevisiae (baker's yeast)	BRR2	NP_011099.1
Eremothecium gossypii	AGOS_AGR113W	NP_986779.1
Neurospora crassa	NCU02685	XP_965458.1
Xenopus tropicalis (tropical clawed frog)	snrnp200	XP_002932581.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001519	XP_321605.3
Magnaporthe oryzae (rice blast fungus)	MGG_15536	XP_003713506.1
Homo sapiens (human)	SNRNP200	NP_054733.2
	SNRNP200	XP_001098299.2
Mus musculus (house mouse)	Snrnp200	NP_796188.2
Rattus norvegicus (Norway rat)	Snrnp200	NP_001032855.2
Danio rerio (zebrafish)	snrnp200	NP_001116729.1
Drosophila melanogaster (fruit fly)	l(3)72Ab	NP_648818.3
Arabidopsis thaliana (thale cress)	emb1507	NP_001185050.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
McKee AE, Minet E, Stern C, Riahi S, Stiles CD, Silver PA
BMC developmental biology514(2005 Jul)

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, F-Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Seino S, Nishimura M, Kaisho T, Hoshino K, Kitamura H, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)

Comparative sequence analysis of a single-gene conserved segment in mouse and human.
Thomas JW, , Green ED
Mammalian genome : official journal of the International Mammalian Genome Society14(10)673-8(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Snrnp200 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Snrnp200 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu80289	XM_017319022.1 Latest version!	Mus musculus small nuclear ribonucleoprotein 200 (U5) (Snrnp200), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu06079	NM_177214.5 Latest version!	Mus musculus small nuclear ribonucleoprotein 200 (U5) (Snrnp200), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1574.30 $2249.00
View Old Accession Versions >>
OMu06079	NM_177214.4	Mus musculus small nuclear ribonucleoprotein 200 (U5) (Snrnp200), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1574.30 $2249.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Snrnp200 ( XM_017319022.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174511.1 Mus musculus small nuclear ribonucleoprotein 200 (U5) (Snrnp200), transcript variant X1, mRNA.

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CloneID	OMu80289
Clone ID Related Accession (Same CDS sequence)	XM_017319022.1
Accession Version	XM_017319022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	U5 small nuclear ribonucleoprotein 200 kDa helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG TGACTGCCCG CAGTTTGCAG TACGAGTACA AGGCGAACTC AAATCTTGTG 
CTCCAAGCTG ACCGCTCTCT CATTGACCGG ACCCGCCGGG ATGAACCGAC GGGAGAGGTT 
CTGTCCCTGG TTGGGAAGTT GGAGGGAACC CGAATGGGAG ATAAGGCTCA ACGGACCAAA 
CCACAGATGC AGGAAGAAAG ACGGGCCAAG AGGAGAAAAC GTGATGAGGA CCGGCATGAC 
ATGAACAAGA TGAAAGGCTA CACGCTGCTG TCTGAGGGCA TTGATGAGAT GGTGGGCATC 
ATCTACAAGC CTAAGACGAA GGAGACCCGA GAGACCTACG AGGTGCTGCT CAGCTTCATC 
CAGGCTGCGC TGGGAGATCA GCCTCGAGAT ATTCTTTGTG GAGCAGCTGA TGAAGTCCTG 
GCTGTGCTGA AGAATGAAAA GCTTCGAGAC AAGGAGAGAA GACGGGAGAT TGACCTGCTG 
CTGGGTCAGA CAGATGATAC CAGATACCAT GTGCTGGTAA ACCTGGGCAA GAAGATCACA 
GACTACGGTG GCGACAAGGA GATACAGAAT ATGGATGACA ACATTGATGA GACTTATGGT 
GTGAACGTGC AGTTTGAGTC TGATGAGGAG GAAGGTGATG AAGATGTGTA TGGGGAAGTC 
CGAGAAGAAG CTTCTGATGA TGACATGGAA GGGGACGAAG CTGTTGTGCG CTGCACCCTC 
TCTGCTAATC TTGTAGCCTC AGGCGAACTG ATGAGTTCTA AGAAGAAGGA TTTGCACCCT 
CGGGATATCG ATGCCTTCTG GCTGCAGCGA CAGCTCAGTC GTTTCTATGA TGATGCCATT 
GTGTCACAGA AGAAGGCAGA TGAGGTGTTG GAGATTTTGA AGACAGCAAG TGATGACCGA 
GAATGTGAGA ACCAACTGGT TTTGCTCCTT GGTTTCAACA CGTTTGACTT CATTAAAGTG 
TTGCGGCAGC ACAGGATGAT GATTTTATAC TGTACCTTGT TGGCCAGTGC TCAGAGTGAA 
CCTGAAAAGG AGAGGATCGT GGGAAAGATG GAAGCTGATC CAGAGCTCTC CAAGTTCCTC 
TATCAGCTCC ATGAGACTGA GAAGGAGGAC CTGATCAGAG AAGAAAGGTC CCGGAGAGAG 
AGAGTCCGTC AGTCCCGAAT GGACACAGAT CTGGAAACCA TGGATTTGGA CCAGGGTGGA 
GAGGCTCTGG CTCCAAGGCA GGTTCTGGAT TTGGAGGACC TTGTTTTTAC CCAAGGAAGC 
CACTTCATGG CCAATAAGCG CTGCCAGCTT CCTGACGGGT CCTTCCGACG CCAGCGTAAA 
GGCTATGAAG AGGTGCATGT GCCTGCTTTG AAGCCCAAGC CCTTTGGCTC AGAAGAACAA 
TTGCTCCCGG TGGAGAAGCT GCCAAAGTAT GCCCAGGCAG GATTTGAGGG CTTCAAAACG 
CTGAACCGGA TCCAGAGTAA GCTCTACCGT GCTGCCCTGG AGACTGATGA GAACCTGCTG 
CTGTGTGCTC CCACTGGTGC GGGCAAGACC AACGTGGCCC TGATGTGTAT GCTCCGAGAG 
ATAGGGAAAC ACATCAACAT GGACGGCACA ATCAATGTGG ATGACTTCAA GATCATCTAC 
ATAGCTCCCA TGCGGTCCCT GGTCCAGGAG ATGGTGGGCA GTTTTGGAAA GCGCCTGGCC 
ACATATGGCA TCACTGTTGC TGAGCTGACT GGGGATCACC AGCTATGCAA GGAGGAAATC 
AGTGCCACAC AGATTATCGT CTGCACCCCT GAGAAGTGGG ACATCATCAC ACGCAAGGGC 
GGGGAGCGCA CCTACACCCA GCTGGTGCGA CTCATTGTCT TGGATGAGAT CCATCTTCTA 
CATGATGACA GAGGTCCTGT CTTAGAAGCT TTGGTGGCCA GGGCCATCCG AAACATTGAG 
ATGACCCAAG AAGATGTCCG ACTCATTGGT CTCAGTGCTA CCCTCCCCAA CTATGAAGAT 
GTGGCCACCT TTCTGCGAGT CGACCCTGCT AAGGGCCTCT TCTACTTTGA TAACAGCTTC 
CGCCCCGTGC CTCTGGAACA AACATATGTG GGCATCACAG AGAAAAAAGC TATCAAACGT 
TTCCAGATCA TGAATGAAAT AGTCTATGAG AAAATCATGG AACATGCTGG AAAAAATCAG 
GTGCTCGTGT TTGTCCATTC TCGAAAAGAA ACTGGGAAGA CAGCAAGGGC AATCCGTGAC 
ATGTGTCTGG AGAAGGACAC TTTGGGTCTG TTTCTTCGCG AGGGTTCTGC CTCCACTGAA 
GTCCTTCGTA CAGAAGCAGA GCAGTGCAAG AACTTGGAGC TGAAGGATCT TTTGCCCTAT 
GGCTTTGCTA TTCATCATGC AGGCATGACT AGAGTTGACC GAACACTTGT AGAAGATCTT 
TTTGCTGACA AGCATATTCA GGTTTTAGTT TCCACCGCAA CTCTGGCGTG GGGTGTAAAT 
CTTCCTGCAC ATACAGTCAT CATTAAAGGT ACCCAAGTGT ACAGTCCAGA GAAGGGGCGT 
TGGACAGAGC TGGGAGCACT GGATATCCTG CAGATGCTGG GCCGTGCTGG ACGGCCGCAG 
TATGACACCA AGGGTGAAGG CATCCTCATC ACATCCCATG GGGAGCTCCA GTACTACCTC 
TCCCTCCTCA ACCAGCAGCT GCCTATCGAG AGCCAGATGG TCTCCAAGCT GCCTGACATG 
CTCAATGCGG AAATTGTTCT GGGCAATGTC CAGAATGCAA AGGATGCAGT GAACTGGCTG 
GGCTATGCCT ACCTATACAT CCGAATGCTC CGGTCCCCTA CCCTCTATGG CATTTCTCAT 
GATGACCTCA AGGGAGATCC CTTGCTGGAC CAGCGCCGAC TCGATCTTGT TCACACTGCT 
GCCTTGATGC TGGACAAGAA CAATCTGGTC AAGTACGACA AGAAGACAGG CAACTTCCAG 
GTGACAGAAC TTGGCCGGAT AGCAAGTCAC TACTATATCA CCAATGATAC TGTGCAGACC 
TACAACCAGC TGCTGAAGCC TACTCTGAGT GAGATTGAGC TTTTCCGAGT GTTCTCCTTG 
TCCTCAGAGT TCAAGAACAT CACTGTAAGA GAGGAGGAGA AGCTGGAGCT GCAGAAGTTG 
CTGGAGAGAG TGCCCATCCC TGTAAAGGAG AGCATTGAGG AACCCAGCGC TAAGATCAAC 
GTGCTTCTCC AAGCCTTCAT CTCACAGCTG AAACTCGAAG GCTTTGCGCT GATGGCTGAC 
ATGGTGTATG TGACCCAGTC GGCTGGCCGG TTGATGCGTG CAATCTTCGA AATTGTCCTG 
AACCGAGGTT GGGCACAGCT TACAGATAAG ACCCTGAATC TCTGCAAGAT GATTGACAAG 
CGCATGTGGC AGTCCATGTG TCCTCTTCGC CAGTTCCGAA AACTTCCTGA GGAAGTAGTG 
AAGAAGATTG AGAAGAAAAA CTTCCCCTTT GAGCGGCTGT ATGACTTGAA TCATAATGAG 
ATAGGTGAAC TTATTCGAAT GCCGAAGATG GGGAAGACCA TCCACAAGTA TGTCCATCTT 
TTCCCCAAGT TGGAGTTGTC AGTGCACCTG CAGCCTATTA CACGCTCTAC GCTGAAAGTA 
GAGCTGACTA TCACACCAGA TTTCCAGTGG GATGAAAAGG CAAGGCTTGA TGGTCAGATG 
GGTGGAGTTG GAGTGCCTGA GTCTCCCTCA CTGGCTCGTC AGGATTTCTT TCAACCATGC 
TTGGGTTGGC AGGTCCATGG TTCGTCAGAG GCATTTTGGA TTCTGGTGGA GGATGTGGAC 
AGTGAGGTGA TTCTGCACCA TGAATATTTT CTGCTGAAGG CCAAGTATGC CCAGGATGAG 
CACCTCATCA CGTTCTTTGT TCCAGTCTTT GAACCACTAC CTCCTCAGTA CTTCATTCGA 
GTAGTGTCTG ATCGCTGGCT CTCTTGTGAG ACGCAGCTAC CTGTCTCCTT CCGGCATCTG 
ATCCTACCAG AGAAGTACCC ACCTCCAACC GAACTGTTGG ACCTGCAGCC ATTGCCTGTG 
TCTGCTCTGA GAAACAGTGC TTTTGAGAGC CTTTACCAAG ATAAATTTCC TTTCTTCAAT 
CCCATCCAGA CTCAAGTATT TAATACCGTG TACAACAGTG ATGATAACGT GTTTGTGGGG 
GCCCCCACGG GCAGCGGGAA GACTATCTGC GCGGAGTTTG CCATCCTTCG GATGCTGCTG 
CAGAATTCTG AGGGACGCTG TGTCTACATT ACCCCTATGG AGGCTCTGGC AGAGCAGGTA 
TACATGGACT GGTATGAGAA GTTTCAGGAC AGGCTCAACA AGAAGGTAGT GCTGCTGACG 
GGGGAGACCA GCACAGATCT GAAGCTCCTG GGCAAAGGCA ACATCATCAT CAGTACCCCT 
GAGAAGTGGG ACATCCTCTC TCGGAGGTGG AAGCAGCGCA AGAACGTCCA GAACATTAAC 
CTCTTTGTGG TGGATGAGGT CCACCTTATT GGGGGCGAGA ATGGGCCTGT CTTGGAAGTG 
ATCTGCTCCC GGATGCGCTA CATCTCCTCC CAGATTGAGC GGCCCATTCG CATTGTGGCA 
CTTAGCTCCT CACTCTCCAA TGCCAAGGAT GTGGCTCACT GGCTGGGCTG CAGTGCCACC 
TCCACCTTCA ACTTCCATCC TAATGTGCGC CCTGTACCTT TGGAACTGCA CATCCAGGGC 
TTCAACATCA GTCACACACA GACTCGCCTG CTCTCTATGG CCAAGCCTGT GTACCATGCT 
ATCACCAAAC ACTCACCCAA GAAGCCTGTC ATCGTTTTTG TCCCATCTCG TAAGCAGACC 
CGCCTCACTG CAATAGACAT CCTCACTACC TGTGCAGCAG ACATCCAGCG GCAGAGGTTC 
CTGCACTGCA CCGAGAAGGA CCTGATCCCT TACCTGGAGA AGCTCAGTGA CAGCACACTC 
AAAGAGACCC TGTTAAATGG GGTGGGCTAC CTGCATGAAG GCCTGAGCCC CATGGAGAGG 
CGCCTGGTAG AGCAGCTCTT CAGCTCCGGG GCTATCCAGG TGGTGGTAGC TTCTCGGAGT 
CTCTGCTGGG GCATGAATGT TGCTGCTCAT CTAGTGATCA TCATGGATAC TCAGTACTAC 
AATGGCAAGA TCCATGCCTA TGTGGATTAC CCCATCTATG ATGTGCTTCA GATGGTGGGC 
CATGCCAACC GGCCCCTGCA GGATGATGAG GGGCGCTGTG TCATCATGTG TCAGGGCTCT 
AAAAAGGATT TTTTCAAAAA ATTTTTGTAT GAGCCATTGC CAGTAGAGTC TCACCTGGAC 
CACTGTATGC ATGACCACTT CAATGCTGAG ATTGTCACCA AGACCATTGA GAACAAGCAG 
GATGCTGTGG ACTACCTCAC CTGGACCTTT CTGTATCGCA GAATGACACA GAACCCCAAT 
TACTACAACC TGCAGGGGAT ATCCCATCGT CATCTGTCTG ACCACCTGTC AGAGCTGGTG 
GAGCAGACCC TCAGTGACCT GGAGCAGTCC AAATGCATCA GTATTGAGGA CGAGATGGAT 
GTGGCCCCTC TGAACCTGGG CATGATTGCT GCCTACTATT ACATAAACTA CACCACCATT 
GAGCTCTTCA GCATGTCTCT GAATGCTAAA ACCAAGGTTC GAGGACTTAT TGAGATCATT 
TCCAATGCAG CAGAGTATGA GAACATTCCA ATCAGGCATC ATGAAGACAA CCTCCTGCGC 
CAGTTGGCTC AGAAGGTCCC CCACAAGCTG AATAACCCCA AGTTCAATGA TCCACATGTG 
AAGACCAATC TGCTGCTGCA GGCTCACCTG TCCCGCATGC AGCTAAGTGC TGAACTACAG 
TCAGACACAG AGGAGATCCT TAGTAAGGCA ATCCGGCTAA TTCAGGCCTG TGTGGATGTA 
CTCTCCAGCA ATGGGTGGCT TAGTCCTGCT CTGGCAGCCA TGGAACTGGC CCAGATGGTC 
ACCCAAGCCA TGTGGTCTAA GGACTCTTAC CTGAAGCAGC TGCCACACTT CACCTCAGAG 
CATATCAAAC GTTGCACAGA TAAGGGAGTG GAGAGTGTTT TTGACATCAT GGAGATGGAG 
GATGAAGAAC GGAATGCATT GCTTCAGTTG ACTGACAGCC AGATTGCAGA TGTGGCCCGC 
TTCTGTAACC GCTACCCGAA TATTGAACTG TCCTATGAAG TGGTGGATAA AGACAGCATC 
CGCAGTGGCG GACCAGTTGT GGTGCTAGTG CAACTGGAGC GAGAGGAGGA AGTCACGGGC 
CCAGTTATTG CACCTCTCTT CCCACAGAAA CGTGAAGAGG GCTGGTGGGT TGTGATTGGA 
GACGCCAAGT CCAACAGCCT CATCTCCATC AAGAGGCTGA CCCTGCAGCA GAAAGCCAAG 
GTGAAGCTAG ACTTTGTGGC CCCAGCCACA GGTGGCCACA ACTATACCCT GTACTTCATG 
AGTGACGCAT ACATGGGCTG TGACCAGGAG TATAAGTTCA GTGTGGATGT GAAAGAAGCT 
GAGACAGACA GTGATTCAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174511.1
CDS	136..6639
Translation	MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTKPQMQEERRAKRRKRDEDRHDMNKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFIQAALGDQPRDILCGAADEVLAVLKNEKLRDKERRREIDLLLGQTDDTRYHVLVNLGKKITDYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTLSANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEPEKERIVGKMEADPELSKFLYQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIVLDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQWDEKARLDGQMGGVGVPESPSLARQDFFQPCLGWQVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQNSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGGHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD

Target ORF information:

RefSeq Version	XM_017319022.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus small nuclear ribonucleoprotein 200 (U5) (Snrnp200), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319022.1

ORF Insert Sequence:

ATGGCGGATG TGACTGCCCG CAGTTTGCAG TACGAGTACA AGGCGAACTC AAATCTTGTG 
CTCCAAGCTG ACCGCTCTCT CATTGACCGG ACCCGCCGGG ATGAACCGAC GGGAGAGGTT 
CTGTCCCTGG TTGGGAAGTT GGAGGGAACC CGAATGGGAG ATAAGGCTCA ACGGACCAAA 
CCACAGATGC AGGAAGAAAG ACGGGCCAAG AGGAGAAAAC GTGATGAGGA CCGGCATGAC 
ATGAACAAGA TGAAAGGCTA CACGCTGCTG TCTGAGGGCA TTGATGAGAT GGTGGGCATC 
ATCTACAAGC CTAAGACGAA GGAGACCCGA GAGACCTACG AGGTGCTGCT CAGCTTCATC 
CAGGCTGCGC TGGGAGATCA GCCTCGAGAT ATTCTTTGTG GAGCAGCTGA TGAAGTCCTG 
GCTGTGCTGA AGAATGAAAA GCTTCGAGAC AAGGAGAGAA GACGGGAGAT TGACCTGCTG 
CTGGGTCAGA CAGATGATAC CAGATACCAT GTGCTGGTAA ACCTGGGCAA GAAGATCACA 
GACTACGGTG GCGACAAGGA GATACAGAAT ATGGATGACA ACATTGATGA GACTTATGGT 
GTGAACGTGC AGTTTGAGTC TGATGAGGAG GAAGGTGATG AAGATGTGTA TGGGGAAGTC 
CGAGAAGAAG CTTCTGATGA TGACATGGAA GGGGACGAAG CTGTTGTGCG CTGCACCCTC 
TCTGCTAATC TTGTAGCCTC AGGCGAACTG ATGAGTTCTA AGAAGAAGGA TTTGCACCCT 
CGGGATATCG ATGCCTTCTG GCTGCAGCGA CAGCTCAGTC GTTTCTATGA TGATGCCATT 
GTGTCACAGA AGAAGGCAGA TGAGGTGTTG GAGATTTTGA AGACAGCAAG TGATGACCGA 
GAATGTGAGA ACCAACTGGT TTTGCTCCTT GGTTTCAACA CGTTTGACTT CATTAAAGTG 
TTGCGGCAGC ACAGGATGAT GATTTTATAC TGTACCTTGT TGGCCAGTGC TCAGAGTGAA 
CCTGAAAAGG AGAGGATCGT GGGAAAGATG GAAGCTGATC CAGAGCTCTC CAAGTTCCTC 
TATCAGCTCC ATGAGACTGA GAAGGAGGAC CTGATCAGAG AAGAAAGGTC CCGGAGAGAG 
AGAGTCCGTC AGTCCCGAAT GGACACAGAT CTGGAAACCA TGGATTTGGA CCAGGGTGGA 
GAGGCTCTGG CTCCAAGGCA GGTTCTGGAT TTGGAGGACC TTGTTTTTAC CCAAGGAAGC 
CACTTCATGG CCAATAAGCG CTGCCAGCTT CCTGACGGGT CCTTCCGACG CCAGCGTAAA 
GGCTATGAAG AGGTGCATGT GCCTGCTTTG AAGCCCAAGC CCTTTGGCTC AGAAGAACAA 
TTGCTCCCGG TGGAGAAGCT GCCAAAGTAT GCCCAGGCAG GATTTGAGGG CTTCAAAACG 
CTGAACCGGA TCCAGAGTAA GCTCTACCGT GCTGCCCTGG AGACTGATGA GAACCTGCTG 
CTGTGTGCTC CCACTGGTGC GGGCAAGACC AACGTGGCCC TGATGTGTAT GCTCCGAGAG 
ATAGGGAAAC ACATCAACAT GGACGGCACA ATCAATGTGG ATGACTTCAA GATCATCTAC 
ATAGCTCCCA TGCGGTCCCT GGTCCAGGAG ATGGTGGGCA GTTTTGGAAA GCGCCTGGCC 
ACATATGGCA TCACTGTTGC TGAGCTGACT GGGGATCACC AGCTATGCAA GGAGGAAATC 
AGTGCCACAC AGATTATCGT CTGCACCCCT GAGAAGTGGG ACATCATCAC ACGCAAGGGC 
GGGGAGCGCA CCTACACCCA GCTGGTGCGA CTCATTGTCT TGGATGAGAT CCATCTTCTA 
CATGATGACA GAGGTCCTGT CTTAGAAGCT TTGGTGGCCA GGGCCATCCG AAACATTGAG 
ATGACCCAAG AAGATGTCCG ACTCATTGGT CTCAGTGCTA CCCTCCCCAA CTATGAAGAT 
GTGGCCACCT TTCTGCGAGT CGACCCTGCT AAGGGCCTCT TCTACTTTGA TAACAGCTTC 
CGCCCCGTGC CTCTGGAACA AACATATGTG GGCATCACAG AGAAAAAAGC TATCAAACGT 
TTCCAGATCA TGAATGAAAT AGTCTATGAG AAAATCATGG AACATGCTGG AAAAAATCAG 
GTGCTCGTGT TTGTCCATTC TCGAAAAGAA ACTGGGAAGA CAGCAAGGGC AATCCGTGAC 
ATGTGTCTGG AGAAGGACAC TTTGGGTCTG TTTCTTCGCG AGGGTTCTGC CTCCACTGAA 
GTCCTTCGTA CAGAAGCAGA GCAGTGCAAG AACTTGGAGC TGAAGGATCT TTTGCCCTAT 
GGCTTTGCTA TTCATCATGC AGGCATGACT AGAGTTGACC GAACACTTGT AGAAGATCTT 
TTTGCTGACA AGCATATTCA GGTTTTAGTT TCCACCGCAA CTCTGGCGTG GGGTGTAAAT 
CTTCCTGCAC ATACAGTCAT CATTAAAGGT ACCCAAGTGT ACAGTCCAGA GAAGGGGCGT 
TGGACAGAGC TGGGAGCACT GGATATCCTG CAGATGCTGG GCCGTGCTGG ACGGCCGCAG 
TATGACACCA AGGGTGAAGG CATCCTCATC ACATCCCATG GGGAGCTCCA GTACTACCTC 
TCCCTCCTCA ACCAGCAGCT GCCTATCGAG AGCCAGATGG TCTCCAAGCT GCCTGACATG 
CTCAATGCGG AAATTGTTCT GGGCAATGTC CAGAATGCAA AGGATGCAGT GAACTGGCTG 
GGCTATGCCT ACCTATACAT CCGAATGCTC CGGTCCCCTA CCCTCTATGG CATTTCTCAT 
GATGACCTCA AGGGAGATCC CTTGCTGGAC CAGCGCCGAC TCGATCTTGT TCACACTGCT 
GCCTTGATGC TGGACAAGAA CAATCTGGTC AAGTACGACA AGAAGACAGG CAACTTCCAG 
GTGACAGAAC TTGGCCGGAT AGCAAGTCAC TACTATATCA CCAATGATAC TGTGCAGACC 
TACAACCAGC TGCTGAAGCC TACTCTGAGT GAGATTGAGC TTTTCCGAGT GTTCTCCTTG 
TCCTCAGAGT TCAAGAACAT CACTGTAAGA GAGGAGGAGA AGCTGGAGCT GCAGAAGTTG 
CTGGAGAGAG TGCCCATCCC TGTAAAGGAG AGCATTGAGG AACCCAGCGC TAAGATCAAC 
GTGCTTCTCC AAGCCTTCAT CTCACAGCTG AAACTCGAAG GCTTTGCGCT GATGGCTGAC 
ATGGTGTATG TGACCCAGTC GGCTGGCCGG TTGATGCGTG CAATCTTCGA AATTGTCCTG 
AACCGAGGTT GGGCACAGCT TACAGATAAG ACCCTGAATC TCTGCAAGAT GATTGACAAG 
CGCATGTGGC AGTCCATGTG TCCTCTTCGC CAGTTCCGAA AACTTCCTGA GGAAGTAGTG 
AAGAAGATTG AGAAGAAAAA CTTCCCCTTT GAGCGGCTGT ATGACTTGAA TCATAATGAG 
ATAGGTGAAC TTATTCGAAT GCCGAAGATG GGGAAGACCA TCCACAAGTA TGTCCATCTT 
TTCCCCAAGT TGGAGTTGTC AGTGCACCTG CAGCCTATTA CACGCTCTAC GCTGAAAGTA 
GAGCTGACTA TCACACCAGA TTTCCAGTGG GATGAAAAGG CAAGGCTTGA TGGTCAGATG 
GGTGGAGTTG GAGTGCCTGA GTCTCCCTCA CTGGCTCGTC AGGATTTCTT TCAACCATGC 
TTGGGTTGGC AGGTCCATGG TTCGTCAGAG GCATTTTGGA TTCTGGTGGA GGATGTGGAC 
AGTGAGGTGA TTCTGCACCA TGAATATTTT CTGCTGAAGG CCAAGTATGC CCAGGATGAG 
CACCTCATCA CGTTCTTTGT TCCAGTCTTT GAACCACTAC CTCCTCAGTA CTTCATTCGA 
GTAGTGTCTG ATCGCTGGCT CTCTTGTGAG ACGCAGCTAC CTGTCTCCTT CCGGCATCTG 
ATCCTACCAG AGAAGTACCC ACCTCCAACC GAACTGTTGG ACCTGCAGCC ATTGCCTGTG 
TCTGCTCTGA GAAACAGTGC TTTTGAGAGC CTTTACCAAG ATAAATTTCC TTTCTTCAAT 
CCCATCCAGA CTCAAGTATT TAATACCGTG TACAACAGTG ATGATAACGT GTTTGTGGGG 
GCCCCCACGG GCAGCGGGAA GACTATCTGC GCGGAGTTTG CCATCCTTCG GATGCTGCTG 
CAGAATTCTG AGGGACGCTG TGTCTACATT ACCCCTATGG AGGCTCTGGC AGAGCAGGTA 
TACATGGACT GGTATGAGAA GTTTCAGGAC AGGCTCAACA AGAAGGTAGT GCTGCTGACG 
GGGGAGACCA GCACAGATCT GAAGCTCCTG GGCAAAGGCA ACATCATCAT CAGTACCCCT 
GAGAAGTGGG ACATCCTCTC TCGGAGGTGG AAGCAGCGCA AGAACGTCCA GAACATTAAC 
CTCTTTGTGG TGGATGAGGT CCACCTTATT GGGGGCGAGA ATGGGCCTGT CTTGGAAGTG 
ATCTGCTCCC GGATGCGCTA CATCTCCTCC CAGATTGAGC GGCCCATTCG CATTGTGGCA 
CTTAGCTCCT CACTCTCCAA TGCCAAGGAT GTGGCTCACT GGCTGGGCTG CAGTGCCACC 
TCCACCTTCA ACTTCCATCC TAATGTGCGC CCTGTACCTT TGGAACTGCA CATCCAGGGC 
TTCAACATCA GTCACACACA GACTCGCCTG CTCTCTATGG CCAAGCCTGT GTACCATGCT 
ATCACCAAAC ACTCACCCAA GAAGCCTGTC ATCGTTTTTG TCCCATCTCG TAAGCAGACC 
CGCCTCACTG CAATAGACAT CCTCACTACC TGTGCAGCAG ACATCCAGCG GCAGAGGTTC 
CTGCACTGCA CCGAGAAGGA CCTGATCCCT TACCTGGAGA AGCTCAGTGA CAGCACACTC 
AAAGAGACCC TGTTAAATGG GGTGGGCTAC CTGCATGAAG GCCTGAGCCC CATGGAGAGG 
CGCCTGGTAG AGCAGCTCTT CAGCTCCGGG GCTATCCAGG TGGTGGTAGC TTCTCGGAGT 
CTCTGCTGGG GCATGAATGT TGCTGCTCAT CTAGTGATCA TCATGGATAC TCAGTACTAC 
AATGGCAAGA TCCATGCCTA TGTGGATTAC CCCATCTATG ATGTGCTTCA GATGGTGGGC 
CATGCCAACC GGCCCCTGCA GGATGATGAG GGGCGCTGTG TCATCATGTG TCAGGGCTCT 
AAAAAGGATT TTTTCAAAAA ATTTTTGTAT GAGCCATTGC CAGTAGAGTC TCACCTGGAC 
CACTGTATGC ATGACCACTT CAATGCTGAG ATTGTCACCA AGACCATTGA GAACAAGCAG 
GATGCTGTGG ACTACCTCAC CTGGACCTTT CTGTATCGCA GAATGACACA GAACCCCAAT 
TACTACAACC TGCAGGGGAT ATCCCATCGT CATCTGTCTG ACCACCTGTC AGAGCTGGTG 
GAGCAGACCC TCAGTGACCT GGAGCAGTCC AAATGCATCA GTATTGAGGA CGAGATGGAT 
GTGGCCCCTC TGAACCTGGG CATGATTGCT GCCTACTATT ACATAAACTA CACCACCATT 
GAGCTCTTCA GCATGTCTCT GAATGCTAAA ACCAAGGTTC GAGGACTTAT TGAGATCATT 
TCCAATGCAG CAGAGTATGA GAACATTCCA ATCAGGCATC ATGAAGACAA CCTCCTGCGC 
CAGTTGGCTC AGAAGGTCCC CCACAAGCTG AATAACCCCA AGTTCAATGA TCCACATGTG 
AAGACCAATC TGCTGCTGCA GGCTCACCTG TCCCGCATGC AGCTAAGTGC TGAACTACAG 
TCAGACACAG AGGAGATCCT TAGTAAGGCA ATCCGGCTAA TTCAGGCCTG TGTGGATGTA 
CTCTCCAGCA ATGGGTGGCT TAGTCCTGCT CTGGCAGCCA TGGAACTGGC CCAGATGGTC 
ACCCAAGCCA TGTGGTCTAA GGACTCTTAC CTGAAGCAGC TGCCACACTT CACCTCAGAG 
CATATCAAAC GTTGCACAGA TAAGGGAGTG GAGAGTGTTT TTGACATCAT GGAGATGGAG 
GATGAAGAAC GGAATGCATT GCTTCAGTTG ACTGACAGCC AGATTGCAGA TGTGGCCCGC 
TTCTGTAACC GCTACCCGAA TATTGAACTG TCCTATGAAG TGGTGGATAA AGACAGCATC 
CGCAGTGGCG GACCAGTTGT GGTGCTAGTG CAACTGGAGC GAGAGGAGGA AGTCACGGGC 
CCAGTTATTG CACCTCTCTT CCCACAGAAA CGTGAAGAGG GCTGGTGGGT TGTGATTGGA 
GACGCCAAGT CCAACAGCCT CATCTCCATC AAGAGGCTGA CCCTGCAGCA GAAAGCCAAG 
GTGAAGCTAG ACTTTGTGGC CCCAGCCACA GGTGGCCACA ACTATACCCT GTACTTCATG 
AGTGACGCAT ACATGGGCTG TGACCAGGAG TATAAGTTCA GTGTGGATGT GAAAGAAGCT 
GAGACAGACA GTGATTCAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Snrnp200 ( NM_177214.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796188.2 Mus musculus small nuclear ribonucleoprotein 200 (U5) (Snrnp200), mRNA.

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CloneID	OMu06079
Clone ID Related Accession (Same CDS sequence)	NM_177214.4 , NM_177214.5
Accession Version	NM_177214.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	U5 small nuclear ribonucleoprotein 200 kDa helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL845368.6. On Nov 17, 2006 this sequence version replaced NM_177214.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC063261.1, SRR1660817.190241.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGATG TGACTGCCCG CAGTTTGCAG TACGAGTACA AGGCGAACTC AAATCTTGTG 
CTCCAAGCTG ACCGCTCTCT CATTGACCGG ACCCGCCGGG ATGAACCGAC GGGAGAGGTT 
CTGTCCCTGG TTGGGAAGTT GGAGGGAACC CGAATGGGAG ATAAGGCTCA ACGGACCAAA 
CCACAGATGC AGGAAGAAAG ACGGGCCAAG AGGAGAAAAC GTGATGAGGA CCGGCATGAC 
ATGAACAAGA TGAAAGGCTA CACGCTGCTG TCTGAGGGCA TTGATGAGAT GGTGGGCATC 
ATCTACAAGC CTAAGACGAA GGAGACCCGA GAGACCTACG AGGTGCTGCT CAGCTTCATC 
CAGGCTGCGC TGGGAGATCA GCCTCGAGAT ATTCTTTGTG GAGCAGCTGA TGAAGTCCTG 
GCTGTGCTGA AGAATGAAAA GCTTCGAGAC AAGGAGAGAA GACGGGAGAT TGACCTGCTG 
CTGGGTCAGA CAGATGATAC CAGATACCAT GTGCTGGTAA ACCTGGGCAA GAAGATCACA 
GACTACGGTG GCGACAAGGA GATACAGAAT ATGGATGACA ACATTGATGA GACTTATGGT 
GTGAACGTGC AGTTTGAGTC TGATGAGGAG GAAGGTGATG AAGATGTGTA TGGGGAAGTC 
CGAGAAGAAG CTTCTGATGA TGACATGGAA GGGGACGAAG CTGTTGTGCG CTGCACCCTC 
TCTGCTAATC TTGTAGCCTC AGGCGAACTG ATGAGTTCTA AGAAGAAGGA TTTGCACCCT 
CGGGATATCG ATGCCTTCTG GCTGCAGCGA CAGCTCAGTC GTTTCTATGA TGATGCCATT 
GTGTCACAGA AGAAGGCAGA TGAGGTGTTG GAGATTTTGA AGACAGCAAG TGATGACCGA 
GAATGTGAGA ACCAACTGGT TTTGCTCCTT GGTTTCAACA CGTTTGACTT CATTAAAGTG 
TTGCGGCAGC ACAGGATGAT GATTTTATAC TGTACCTTGT TGGCCAGTGC TCAGAGTGAA 
CCTGAAAAGG AGAGGATCGT GGGAAAGATG GAAGCTGATC CAGAGCTCTC CAAGTTCCTC 
TATCAGCTCC ATGAGACTGA GAAGGAGGAC CTGATCAGAG AAGAAAGGTC CCGGAGAGAG 
AGAGTCCGTC AGTCCCGAAT GGACACAGAT CTGGAAACCA TGGATTTGGA CCAGGGTGGA 
GAGGCTCTGG CTCCAAGGCA GGTTCTGGAT TTGGAGGACC TTGTTTTTAC CCAAGGAAGC 
CACTTCATGG CCAATAAGCG CTGCCAGCTT CCTGACGGGT CCTTCCGACG CCAGCGTAAA 
GGCTATGAAG AGGTGCATGT GCCTGCTTTG AAGCCCAAGC CCTTTGGCTC AGAAGAACAA 
TTGCTCCCGG TGGAGAAGCT GCCAAAGTAT GCCCAGGCAG GATTTGAGGG CTTCAAAACG 
CTGAACCGGA TCCAGAGTAA GCTCTACCGT GCTGCCCTGG AGACTGATGA GAACCTGCTG 
CTGTGTGCTC CCACTGGTGC GGGCAAGACC AACGTGGCCC TGATGTGTAT GCTCCGAGAG 
ATAGGGAAAC ACATCAACAT GGACGGCACA ATCAATGTGG ATGACTTCAA GATCATCTAC 
ATAGCTCCCA TGCGGTCCCT GGTCCAGGAG ATGGTGGGCA GTTTTGGAAA GCGCCTGGCC 
ACATATGGCA TCACTGTTGC TGAGCTGACT GGGGATCACC AGCTATGCAA GGAGGAAATC 
AGTGCCACAC AGATTATCGT CTGCACCCCT GAGAAGTGGG ACATCATCAC ACGCAAGGGC 
GGGGAGCGCA CCTACACCCA GCTGGTGCGA CTCATTGTCT TGGATGAGAT CCATCTTCTA 
CATGATGACA GAGGTCCTGT CTTAGAAGCT TTGGTGGCCA GGGCCATCCG AAACATTGAG 
ATGACCCAAG AAGATGTCCG ACTCATTGGT CTCAGTGCTA CCCTCCCCAA CTATGAAGAT 
GTGGCCACCT TTCTGCGAGT CGACCCTGCT AAGGGCCTCT TCTACTTTGA TAACAGCTTC 
CGCCCCGTGC CTCTGGAACA AACATATGTG GGCATCACAG AGAAAAAAGC TATCAAACGT 
TTCCAGATCA TGAATGAAAT AGTCTATGAG AAAATCATGG AACATGCTGG AAAAAATCAG 
GTGCTCGTGT TTGTCCATTC TCGAAAAGAA ACTGGGAAGA CAGCAAGGGC AATCCGTGAC 
ATGTGTCTGG AGAAGGACAC TTTGGGTCTG TTTCTTCGCG AGGGTTCTGC CTCCACTGAA 
GTCCTTCGTA CAGAAGCAGA GCAGTGCAAG AACTTGGAGC TGAAGGATCT TTTGCCCTAT 
GGCTTTGCTA TTCATCATGC AGGCATGACT AGAGTTGACC GAACACTTGT AGAAGATCTT 
TTTGCTGACA AGCATATTCA GGTTTTAGTT TCCACCGCAA CTCTGGCGTG GGGTGTAAAT 
CTTCCTGCAC ATACAGTCAT CATTAAAGGT ACCCAAGTGT ACAGTCCAGA GAAGGGGCGT 
TGGACAGAGC TGGGAGCACT GGATATCCTG CAGATGCTGG GCCGTGCTGG ACGGCCGCAG 
TATGACACCA AGGGTGAAGG CATCCTCATC ACATCCCATG GGGAGCTCCA GTACTACCTC 
TCCCTCCTCA ACCAGCAGCT GCCTATCGAG AGCCAGATGG TCTCCAAGCT GCCTGACATG 
CTCAATGCGG AAATTGTTCT GGGCAATGTC CAGAATGCAA AGGATGCAGT GAACTGGCTG 
GGCTATGCCT ACCTATACAT CCGAATGCTC CGGTCCCCTA CCCTCTATGG CATTTCTCAT 
GATGACCTCA AGGGAGATCC CTTGCTGGAC CAGCGCCGAC TCGATCTTGT TCACACTGCT 
GCCTTGATGC TGGACAAGAA CAATCTGGTC AAGTACGACA AGAAGACAGG CAACTTCCAG 
GTGACAGAAC TTGGCCGGAT AGCAAGTCAC TACTATATCA CCAATGATAC TGTGCAGACC 
TACAACCAGC TGCTGAAGCC TACTCTGAGT GAGATTGAGC TTTTCCGAGT GTTCTCCTTG 
TCCTCAGAGT TCAAGAACAT CACTGTAAGA GAGGAGGAGA AGCTGGAGCT GCAGAAGTTG 
CTGGAGAGAG TGCCCATCCC TGTAAAGGAG AGCATTGAGG AACCCAGCGC TAAGATCAAC 
GTGCTTCTCC AAGCCTTCAT CTCACAGCTG AAACTCGAAG GCTTTGCGCT GATGGCTGAC 
ATGGTGTATG TGACCCAGTC GGCTGGCCGG TTGATGCGTG CAATCTTCGA AATTGTCCTG 
AACCGAGGTT GGGCACAGCT TACAGATAAG ACCCTGAATC TCTGCAAGAT GATTGACAAG 
CGCATGTGGC AGTCCATGTG TCCTCTTCGC CAGTTCCGAA AACTTCCTGA GGAAGTAGTG 
AAGAAGATTG AGAAGAAAAA CTTCCCCTTT GAGCGGCTGT ATGACTTGAA TCATAATGAG 
ATAGGTGAAC TTATTCGAAT GCCGAAGATG GGGAAGACCA TCCACAAGTA TGTCCATCTT 
TTCCCCAAGT TGGAGTTGTC AGTGCACCTG CAGCCTATTA CACGCTCTAC GCTGAAAGTA 
GAGCTGACTA TCACACCAGA TTTCCAGTGG GATGAAAAGG TCCATGGTTC GTCAGAGGCA 
TTTTGGATTC TGGTGGAGGA TGTGGACAGT GAGGTGATTC TGCACCATGA ATATTTTCTG 
CTGAAGGCCA AGTATGCCCA GGATGAGCAC CTCATCACGT TCTTTGTTCC AGTCTTTGAA 
CCACTACCTC CTCAGTACTT CATTCGAGTA GTGTCTGATC GCTGGCTCTC TTGTGAGACG 
CAGCTACCTG TCTCCTTCCG GCATCTGATC CTACCAGAGA AGTACCCACC TCCAACCGAA 
CTGTTGGACC TGCAGCCATT GCCTGTGTCT GCTCTGAGAA ACAGTGCTTT TGAGAGCCTT 
TACCAAGATA AATTTCCTTT CTTCAATCCC ATCCAGACTC AAGTATTTAA TACCGTGTAC 
AACAGTGATG ATAACGTGTT TGTGGGGGCC CCCACGGGCA GCGGGAAGAC TATCTGCGCG 
GAGTTTGCCA TCCTTCGGAT GCTGCTGCAG AATTCTGAGG GACGCTGTGT CTACATTACC 
CCTATGGAGG CTCTGGCAGA GCAGGTATAC ATGGACTGGT ATGAGAAGTT TCAGGACAGG 
CTCAACAAGA AGGTAGTGCT GCTGACGGGG GAGACCAGCA CAGATCTGAA GCTCCTGGGC 
AAAGGCAACA TCATCATCAG TACCCCTGAG AAGTGGGACA TCCTCTCTCG GAGGTGGAAG 
CAGCGCAAGA ACGTCCAGAA CATTAACCTC TTTGTGGTGG ATGAGGTCCA CCTTATTGGG 
GGCGAGAATG GGCCTGTCTT GGAAGTGATC TGCTCCCGGA TGCGCTACAT CTCCTCCCAG 
ATTGAGCGGC CCATTCGCAT TGTGGCACTT AGCTCCTCAC TCTCCAATGC CAAGGATGTG 
GCTCACTGGC TGGGCTGCAG TGCCACCTCC ACCTTCAACT TCCATCCTAA TGTGCGCCCT 
GTACCTTTGG AACTGCACAT CCAGGGCTTC AACATCAGTC ACACACAGAC TCGCCTGCTC 
TCTATGGCCA AGCCTGTGTA CCATGCTATC ACCAAACACT CACCCAAGAA GCCTGTCATC 
GTTTTTGTCC CATCTCGTAA GCAGACCCGC CTCACTGCAA TAGACATCCT CACTACCTGT 
GCAGCAGACA TCCAGCGGCA GAGGTTCCTG CACTGCACCG AGAAGGACCT GATCCCTTAC 
CTGGAGAAGC TCAGTGACAG CACACTCAAA GAGACCCTGT TAAATGGGGT GGGCTACCTG 
CATGAAGGCC TGAGCCCCAT GGAGAGGCGC CTGGTAGAGC AGCTCTTCAG CTCCGGGGCT 
ATCCAGGTGG TGGTAGCTTC TCGGAGTCTC TGCTGGGGCA TGAATGTTGC TGCTCATCTA 
GTGATCATCA TGGATACTCA GTACTACAAT GGCAAGATCC ATGCCTATGT GGATTACCCC 
ATCTATGATG TGCTTCAGAT GGTGGGCCAT GCCAACCGGC CCCTGCAGGA TGATGAGGGG 
CGCTGTGTCA TCATGTGTCA GGGCTCTAAA AAGGATTTTT TCAAAAAATT TTTGTATGAG 
CCATTGCCAG TAGAGTCTCA CCTGGACCAC TGTATGCATG ACCACTTCAA TGCTGAGATT 
GTCACCAAGA CCATTGAGAA CAAGCAGGAT GCTGTGGACT ACCTCACCTG GACCTTTCTG 
TATCGCAGAA TGACACAGAA CCCCAATTAC TACAACCTGC AGGGGATATC CCATCGTCAT 
CTGTCTGACC ACCTGTCAGA GCTGGTGGAG CAGACCCTCA GTGACCTGGA GCAGTCCAAA 
TGCATCAGTA TTGAGGACGA GATGGATGTG GCCCCTCTGA ACCTGGGCAT GATTGCTGCC 
TACTATTACA TAAACTACAC CACCATTGAG CTCTTCAGCA TGTCTCTGAA TGCTAAAACC 
AAGGTTCGAG GACTTATTGA GATCATTTCC AATGCAGCAG AGTATGAGAA CATTCCAATC 
AGGCATCATG AAGACAACCT CCTGCGCCAG TTGGCTCAGA AGGTCCCCCA CAAGCTGAAT 
AACCCCAAGT TCAATGATCC ACATGTGAAG ACCAATCTGC TGCTGCAGGC TCACCTGTCC 
CGCATGCAGC TAAGTGCTGA ACTACAGTCA GACACAGAGG AGATCCTTAG TAAGGCAATC 
CGGCTAATTC AGGCCTGTGT GGATGTACTC TCCAGCAATG GGTGGCTTAG TCCTGCTCTG 
GCAGCCATGG AACTGGCCCA GATGGTCACC CAAGCCATGT GGTCTAAGGA CTCTTACCTG 
AAGCAGCTGC CACACTTCAC CTCAGAGCAT ATCAAACGTT GCACAGATAA GGGAGTGGAG 
AGTGTTTTTG ACATCATGGA GATGGAGGAT GAAGAACGGA ATGCATTGCT TCAGTTGACT 
GACAGCCAGA TTGCAGATGT GGCCCGCTTC TGTAACCGCT ACCCGAATAT TGAACTGTCC 
TATGAAGTGG TGGATAAAGA CAGCATCCGC AGTGGCGGAC CAGTTGTGGT GCTAGTGCAA 
CTGGAGCGAG AGGAGGAAGT CACGGGCCCA GTTATTGCAC CTCTCTTCCC ACAGAAACGT 
GAAGAGGGCT GGTGGGTTGT GATTGGAGAC GCCAAGTCCA ACAGCCTCAT CTCCATCAAG 
AGGCTGACCC TGCAGCAGAA AGCCAAGGTG AAGCTAGACT TTGTGGCCCC AGCCACAGGT 
GGCCACAACT ATACCCTGTA CTTCATGAGT GACGCATACA TGGGCTGTGA CCAGGAGTAT 
AAGTTCAGTG TGGATGTGAA AGAAGCTGAG ACAGACAGTG ATTCAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796188.2
CDS	91..6501
Translation	MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTKPQMQEERRAKRRKRDEDRHDMNKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFIQAALGDQPRDILCGAADEVLAVLKNEKLRDKERRREIDLLLGQTDDTRYHVLVNLGKKITDYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTLSANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEPEKERIVGKMEADPELSKFLYQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIVLDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQNSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGGHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD

Target ORF information:

RefSeq Version	NM_177214.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus small nuclear ribonucleoprotein 200 (U5) (Snrnp200), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177214.4

ORF Insert Sequence:

ATGGCGGATG TGACTGCCCG CAGTTTGCAG TACGAGTACA AGGCGAACTC AAATCTTGTG 
CTCCAAGCTG ACCGCTCTCT CATTGACCGG ACCCGCCGGG ATGAACCGAC GGGAGAGGTT 
CTGTCCCTGG TTGGGAAGTT GGAGGGAACC CGAATGGGAG ATAAGGCTCA ACGGACCAAA 
CCACAGATGC AGGAAGAAAG ACGGGCCAAG AGGAGAAAAC GTGATGAGGA CCGGCATGAC 
ATGAACAAGA TGAAAGGCTA CACGCTGCTG TCTGAGGGCA TTGATGAGAT GGTGGGCATC 
ATCTACAAGC CTAAGACGAA GGAGACCCGA GAGACCTACG AGGTGCTGCT CAGCTTCATC 
CAGGCTGCGC TGGGAGATCA GCCTCGAGAT ATTCTTTGTG GAGCAGCTGA TGAAGTCCTG 
GCTGTGCTGA AGAATGAAAA GCTTCGAGAC AAGGAGAGAA GACGGGAGAT TGACCTGCTG 
CTGGGTCAGA CAGATGATAC CAGATACCAT GTGCTGGTAA ACCTGGGCAA GAAGATCACA 
GACTACGGTG GCGACAAGGA GATACAGAAT ATGGATGACA ACATTGATGA GACTTATGGT 
GTGAACGTGC AGTTTGAGTC TGATGAGGAG GAAGGTGATG AAGATGTGTA TGGGGAAGTC 
CGAGAAGAAG CTTCTGATGA TGACATGGAA GGGGACGAAG CTGTTGTGCG CTGCACCCTC 
TCTGCTAATC TTGTAGCCTC AGGCGAACTG ATGAGTTCTA AGAAGAAGGA TTTGCACCCT 
CGGGATATCG ATGCCTTCTG GCTGCAGCGA CAGCTCAGTC GTTTCTATGA TGATGCCATT 
GTGTCACAGA AGAAGGCAGA TGAGGTGTTG GAGATTTTGA AGACAGCAAG TGATGACCGA 
GAATGTGAGA ACCAACTGGT TTTGCTCCTT GGTTTCAACA CGTTTGACTT CATTAAAGTG 
TTGCGGCAGC ACAGGATGAT GATTTTATAC TGTACCTTGT TGGCCAGTGC TCAGAGTGAA 
CCTGAAAAGG AGAGGATCGT GGGAAAGATG GAAGCTGATC CAGAGCTCTC CAAGTTCCTC 
TATCAGCTCC ATGAGACTGA GAAGGAGGAC CTGATCAGAG AAGAAAGGTC CCGGAGAGAG 
AGAGTCCGTC AGTCCCGAAT GGACACAGAT CTGGAAACCA TGGATTTGGA CCAGGGTGGA 
GAGGCTCTGG CTCCAAGGCA GGTTCTGGAT TTGGAGGACC TTGTTTTTAC CCAAGGAAGC 
CACTTCATGG CCAATAAGCG CTGCCAGCTT CCTGACGGGT CCTTCCGACG CCAGCGTAAA 
GGCTATGAAG AGGTGCATGT GCCTGCTTTG AAGCCCAAGC CCTTTGGCTC AGAAGAACAA 
TTGCTCCCGG TGGAGAAGCT GCCAAAGTAT GCCCAGGCAG GATTTGAGGG CTTCAAAACG 
CTGAACCGGA TCCAGAGTAA GCTCTACCGT GCTGCCCTGG AGACTGATGA GAACCTGCTG 
CTGTGTGCTC CCACTGGTGC GGGCAAGACC AACGTGGCCC TGATGTGTAT GCTCCGAGAG 
ATAGGGAAAC ACATCAACAT GGACGGCACA ATCAATGTGG ATGACTTCAA GATCATCTAC 
ATAGCTCCCA TGCGGTCCCT GGTCCAGGAG ATGGTGGGCA GTTTTGGAAA GCGCCTGGCC 
ACATATGGCA TCACTGTTGC TGAGCTGACT GGGGATCACC AGCTATGCAA GGAGGAAATC 
AGTGCCACAC AGATTATCGT CTGCACCCCT GAGAAGTGGG ACATCATCAC ACGCAAGGGC 
GGGGAGCGCA CCTACACCCA GCTGGTGCGA CTCATTGTCT TGGATGAGAT CCATCTTCTA 
CATGATGACA GAGGTCCTGT CTTAGAAGCT TTGGTGGCCA GGGCCATCCG AAACATTGAG 
ATGACCCAAG AAGATGTCCG ACTCATTGGT CTCAGTGCTA CCCTCCCCAA CTATGAAGAT 
GTGGCCACCT TTCTGCGAGT CGACCCTGCT AAGGGCCTCT TCTACTTTGA TAACAGCTTC 
CGCCCCGTGC CTCTGGAACA AACATATGTG GGCATCACAG AGAAAAAAGC TATCAAACGT 
TTCCAGATCA TGAATGAAAT AGTCTATGAG AAAATCATGG AACATGCTGG AAAAAATCAG 
GTGCTCGTGT TTGTCCATTC TCGAAAAGAA ACTGGGAAGA CAGCAAGGGC AATCCGTGAC 
ATGTGTCTGG AGAAGGACAC TTTGGGTCTG TTTCTTCGCG AGGGTTCTGC CTCCACTGAA 
GTCCTTCGTA CAGAAGCAGA GCAGTGCAAG AACTTGGAGC TGAAGGATCT TTTGCCCTAT 
GGCTTTGCTA TTCATCATGC AGGCATGACT AGAGTTGACC GAACACTTGT AGAAGATCTT 
TTTGCTGACA AGCATATTCA GGTTTTAGTT TCCACCGCAA CTCTGGCGTG GGGTGTAAAT 
CTTCCTGCAC ATACAGTCAT CATTAAAGGT ACCCAAGTGT ACAGTCCAGA GAAGGGGCGT 
TGGACAGAGC TGGGAGCACT GGATATCCTG CAGATGCTGG GCCGTGCTGG ACGGCCGCAG 
TATGACACCA AGGGTGAAGG CATCCTCATC ACATCCCATG GGGAGCTCCA GTACTACCTC 
TCCCTCCTCA ACCAGCAGCT GCCTATCGAG AGCCAGATGG TCTCCAAGCT GCCTGACATG 
CTCAATGCGG AAATTGTTCT GGGCAATGTC CAGAATGCAA AGGATGCAGT GAACTGGCTG 
GGCTATGCCT ACCTATACAT CCGAATGCTC CGGTCCCCTA CCCTCTATGG CATTTCTCAT 
GATGACCTCA AGGGAGATCC CTTGCTGGAC CAGCGCCGAC TCGATCTTGT TCACACTGCT 
GCCTTGATGC TGGACAAGAA CAATCTGGTC AAGTACGACA AGAAGACAGG CAACTTCCAG 
GTGACAGAAC TTGGCCGGAT AGCAAGTCAC TACTATATCA CCAATGATAC TGTGCAGACC 
TACAACCAGC TGCTGAAGCC TACTCTGAGT GAGATTGAGC TTTTCCGAGT GTTCTCCTTG 
TCCTCAGAGT TCAAGAACAT CACTGTAAGA GAGGAGGAGA AGCTGGAGCT GCAGAAGTTG 
CTGGAGAGAG TGCCCATCCC TGTAAAGGAG AGCATTGAGG AACCCAGCGC TAAGATCAAC 
GTGCTTCTCC AAGCCTTCAT CTCACAGCTG AAACTCGAAG GCTTTGCGCT GATGGCTGAC 
ATGGTGTATG TGACCCAGTC GGCTGGCCGG TTGATGCGTG CAATCTTCGA AATTGTCCTG 
AACCGAGGTT GGGCACAGCT TACAGATAAG ACCCTGAATC TCTGCAAGAT GATTGACAAG 
CGCATGTGGC AGTCCATGTG TCCTCTTCGC CAGTTCCGAA AACTTCCTGA GGAAGTAGTG 
AAGAAGATTG AGAAGAAAAA CTTCCCCTTT GAGCGGCTGT ATGACTTGAA TCATAATGAG 
ATAGGTGAAC TTATTCGAAT GCCGAAGATG GGGAAGACCA TCCACAAGTA TGTCCATCTT 
TTCCCCAAGT TGGAGTTGTC AGTGCACCTG CAGCCTATTA CACGCTCTAC GCTGAAAGTA 
GAGCTGACTA TCACACCAGA TTTCCAGTGG GATGAAAAGG TCCATGGTTC GTCAGAGGCA 
TTTTGGATTC TGGTGGAGGA TGTGGACAGT GAGGTGATTC TGCACCATGA ATATTTTCTG 
CTGAAGGCCA AGTATGCCCA GGATGAGCAC CTCATCACGT TCTTTGTTCC AGTCTTTGAA 
CCACTACCTC CTCAGTACTT CATTCGAGTA GTGTCTGATC GCTGGCTCTC TTGTGAGACG 
CAGCTACCTG TCTCCTTCCG GCATCTGATC CTACCAGAGA AGTACCCACC TCCAACCGAA 
CTGTTGGACC TGCAGCCATT GCCTGTGTCT GCTCTGAGAA ACAGTGCTTT TGAGAGCCTT 
TACCAAGATA AATTTCCTTT CTTCAATCCC ATCCAGACTC AAGTATTTAA TACCGTGTAC 
AACAGTGATG ATAACGTGTT TGTGGGGGCC CCCACGGGCA GCGGGAAGAC TATCTGCGCG 
GAGTTTGCCA TCCTTCGGAT GCTGCTGCAG AATTCTGAGG GACGCTGTGT CTACATTACC 
CCTATGGAGG CTCTGGCAGA GCAGGTATAC ATGGACTGGT ATGAGAAGTT TCAGGACAGG 
CTCAACAAGA AGGTAGTGCT GCTGACGGGG GAGACCAGCA CAGATCTGAA GCTCCTGGGC 
AAAGGCAACA TCATCATCAG TACCCCTGAG AAGTGGGACA TCCTCTCTCG GAGGTGGAAG 
CAGCGCAAGA ACGTCCAGAA CATTAACCTC TTTGTGGTGG ATGAGGTCCA CCTTATTGGG 
GGCGAGAATG GGCCTGTCTT GGAAGTGATC TGCTCCCGGA TGCGCTACAT CTCCTCCCAG 
ATTGAGCGGC CCATTCGCAT TGTGGCACTT AGCTCCTCAC TCTCCAATGC CAAGGATGTG 
GCTCACTGGC TGGGCTGCAG TGCCACCTCC ACCTTCAACT TCCATCCTAA TGTGCGCCCT 
GTACCTTTGG AACTGCACAT CCAGGGCTTC AACATCAGTC ACACACAGAC TCGCCTGCTC 
TCTATGGCCA AGCCTGTGTA CCATGCTATC ACCAAACACT CACCCAAGAA GCCTGTCATC 
GTTTTTGTCC CATCTCGTAA GCAGACCCGC CTCACTGCAA TAGACATCCT CACTACCTGT 
GCAGCAGACA TCCAGCGGCA GAGGTTCCTG CACTGCACCG AGAAGGACCT GATCCCTTAC 
CTGGAGAAGC TCAGTGACAG CACACTCAAA GAGACCCTGT TAAATGGGGT GGGCTACCTG 
CATGAAGGCC TGAGCCCCAT GGAGAGGCGC CTGGTAGAGC AGCTCTTCAG CTCCGGGGCT 
ATCCAGGTGG TGGTAGCTTC TCGGAGTCTC TGCTGGGGCA TGAATGTTGC TGCTCATCTA 
GTGATCATCA TGGATACTCA GTACTACAAT GGCAAGATCC ATGCCTATGT GGATTACCCC 
ATCTATGATG TGCTTCAGAT GGTGGGCCAT GCCAACCGGC CCCTGCAGGA TGATGAGGGG 
CGCTGTGTCA TCATGTGTCA GGGCTCTAAA AAGGATTTTT TCAAAAAATT TTTGTATGAG 
CCATTGCCAG TAGAGTCTCA CCTGGACCAC TGTATGCATG ACCACTTCAA TGCTGAGATT 
GTCACCAAGA CCATTGAGAA CAAGCAGGAT GCTGTGGACT ACCTCACCTG GACCTTTCTG 
TATCGCAGAA TGACACAGAA CCCCAATTAC TACAACCTGC AGGGGATATC CCATCGTCAT 
CTGTCTGACC ACCTGTCAGA GCTGGTGGAG CAGACCCTCA GTGACCTGGA GCAGTCCAAA 
TGCATCAGTA TTGAGGACGA GATGGATGTG GCCCCTCTGA ACCTGGGCAT GATTGCTGCC 
TACTATTACA TAAACTACAC CACCATTGAG CTCTTCAGCA TGTCTCTGAA TGCTAAAACC 
AAGGTTCGAG GACTTATTGA GATCATTTCC AATGCAGCAG AGTATGAGAA CATTCCAATC 
AGGCATCATG AAGACAACCT CCTGCGCCAG TTGGCTCAGA AGGTCCCCCA CAAGCTGAAT 
AACCCCAAGT TCAATGATCC ACATGTGAAG ACCAATCTGC TGCTGCAGGC TCACCTGTCC 
CGCATGCAGC TAAGTGCTGA ACTACAGTCA GACACAGAGG AGATCCTTAG TAAGGCAATC 
CGGCTAATTC AGGCCTGTGT GGATGTACTC TCCAGCAATG GGTGGCTTAG TCCTGCTCTG 
GCAGCCATGG AACTGGCCCA GATGGTCACC CAAGCCATGT GGTCTAAGGA CTCTTACCTG 
AAGCAGCTGC CACACTTCAC CTCAGAGCAT ATCAAACGTT GCACAGATAA GGGAGTGGAG 
AGTGTTTTTG ACATCATGGA GATGGAGGAT GAAGAACGGA ATGCATTGCT TCAGTTGACT 
GACAGCCAGA TTGCAGATGT GGCCCGCTTC TGTAACCGCT ACCCGAATAT TGAACTGTCC 
TATGAAGTGG TGGATAAAGA CAGCATCCGC AGTGGCGGAC CAGTTGTGGT GCTAGTGCAA 
CTGGAGCGAG AGGAGGAAGT CACGGGCCCA GTTATTGCAC CTCTCTTCCC ACAGAAACGT 
GAAGAGGGCT GGTGGGTTGT GATTGGAGAC GCCAAGTCCA ACAGCCTCAT CTCCATCAAG 
AGGCTGACCC TGCAGCAGAA AGCCAAGGTG AAGCTAGACT TTGTGGCCCC AGCCACAGGT 
GGCCACAACT ATACCCTGTA CTTCATGAGT GACGCATACA TGGGCTGTGA CCAGGAGTAT 
AAGTTCAGTG TGGATGTGAA AGAAGCTGAG ACAGACAGTG ATTCAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Snrnp200 ( NM_177214.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796188.2 Mus musculus small nuclear ribonucleoprotein 200 (U5) (Snrnp200), mRNA.

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CloneID	OMu06079
Clone ID Related Accession (Same CDS sequence)	NM_177214.4 , NM_177214.5
Accession Version	NM_177214.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	U5 small nuclear ribonucleoprotein 200 kDa helicase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845368.6. On Apr 26, 2018 this sequence version replaced NM_177214.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC063261.1, SRR1660817.190241.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATG TGACTGCCCG CAGTTTGCAG TACGAGTACA AGGCGAACTC AAATCTTGTG 
CTCCAAGCTG ACCGCTCTCT CATTGACCGG ACCCGCCGGG ATGAACCGAC GGGAGAGGTT 
CTGTCCCTGG TTGGGAAGTT GGAGGGAACC CGAATGGGAG ATAAGGCTCA ACGGACCAAA 
CCACAGATGC AGGAAGAAAG ACGGGCCAAG AGGAGAAAAC GTGATGAGGA CCGGCATGAC 
ATGAACAAGA TGAAAGGCTA CACGCTGCTG TCTGAGGGCA TTGATGAGAT GGTGGGCATC 
ATCTACAAGC CTAAGACGAA GGAGACCCGA GAGACCTACG AGGTGCTGCT CAGCTTCATC 
CAGGCTGCGC TGGGAGATCA GCCTCGAGAT ATTCTTTGTG GAGCAGCTGA TGAAGTCCTG 
GCTGTGCTGA AGAATGAAAA GCTTCGAGAC AAGGAGAGAA GACGGGAGAT TGACCTGCTG 
CTGGGTCAGA CAGATGATAC CAGATACCAT GTGCTGGTAA ACCTGGGCAA GAAGATCACA 
GACTACGGTG GCGACAAGGA GATACAGAAT ATGGATGACA ACATTGATGA GACTTATGGT 
GTGAACGTGC AGTTTGAGTC TGATGAGGAG GAAGGTGATG AAGATGTGTA TGGGGAAGTC 
CGAGAAGAAG CTTCTGATGA TGACATGGAA GGGGACGAAG CTGTTGTGCG CTGCACCCTC 
TCTGCTAATC TTGTAGCCTC AGGCGAACTG ATGAGTTCTA AGAAGAAGGA TTTGCACCCT 
CGGGATATCG ATGCCTTCTG GCTGCAGCGA CAGCTCAGTC GTTTCTATGA TGATGCCATT 
GTGTCACAGA AGAAGGCAGA TGAGGTGTTG GAGATTTTGA AGACAGCAAG TGATGACCGA 
GAATGTGAGA ACCAACTGGT TTTGCTCCTT GGTTTCAACA CGTTTGACTT CATTAAAGTG 
TTGCGGCAGC ACAGGATGAT GATTTTATAC TGTACCTTGT TGGCCAGTGC TCAGAGTGAA 
CCTGAAAAGG AGAGGATCGT GGGAAAGATG GAAGCTGATC CAGAGCTCTC CAAGTTCCTC 
TATCAGCTCC ATGAGACTGA GAAGGAGGAC CTGATCAGAG AAGAAAGGTC CCGGAGAGAG 
AGAGTCCGTC AGTCCCGAAT GGACACAGAT CTGGAAACCA TGGATTTGGA CCAGGGTGGA 
GAGGCTCTGG CTCCAAGGCA GGTTCTGGAT TTGGAGGACC TTGTTTTTAC CCAAGGAAGC 
CACTTCATGG CCAATAAGCG CTGCCAGCTT CCTGACGGGT CCTTCCGACG CCAGCGTAAA 
GGCTATGAAG AGGTGCATGT GCCTGCTTTG AAGCCCAAGC CCTTTGGCTC AGAAGAACAA 
TTGCTCCCGG TGGAGAAGCT GCCAAAGTAT GCCCAGGCAG GATTTGAGGG CTTCAAAACG 
CTGAACCGGA TCCAGAGTAA GCTCTACCGT GCTGCCCTGG AGACTGATGA GAACCTGCTG 
CTGTGTGCTC CCACTGGTGC GGGCAAGACC AACGTGGCCC TGATGTGTAT GCTCCGAGAG 
ATAGGGAAAC ACATCAACAT GGACGGCACA ATCAATGTGG ATGACTTCAA GATCATCTAC 
ATAGCTCCCA TGCGGTCCCT GGTCCAGGAG ATGGTGGGCA GTTTTGGAAA GCGCCTGGCC 
ACATATGGCA TCACTGTTGC TGAGCTGACT GGGGATCACC AGCTATGCAA GGAGGAAATC 
AGTGCCACAC AGATTATCGT CTGCACCCCT GAGAAGTGGG ACATCATCAC ACGCAAGGGC 
GGGGAGCGCA CCTACACCCA GCTGGTGCGA CTCATTGTCT TGGATGAGAT CCATCTTCTA 
CATGATGACA GAGGTCCTGT CTTAGAAGCT TTGGTGGCCA GGGCCATCCG AAACATTGAG 
ATGACCCAAG AAGATGTCCG ACTCATTGGT CTCAGTGCTA CCCTCCCCAA CTATGAAGAT 
GTGGCCACCT TTCTGCGAGT CGACCCTGCT AAGGGCCTCT TCTACTTTGA TAACAGCTTC 
CGCCCCGTGC CTCTGGAACA AACATATGTG GGCATCACAG AGAAAAAAGC TATCAAACGT 
TTCCAGATCA TGAATGAAAT AGTCTATGAG AAAATCATGG AACATGCTGG AAAAAATCAG 
GTGCTCGTGT TTGTCCATTC TCGAAAAGAA ACTGGGAAGA CAGCAAGGGC AATCCGTGAC 
ATGTGTCTGG AGAAGGACAC TTTGGGTCTG TTTCTTCGCG AGGGTTCTGC CTCCACTGAA 
GTCCTTCGTA CAGAAGCAGA GCAGTGCAAG AACTTGGAGC TGAAGGATCT TTTGCCCTAT 
GGCTTTGCTA TTCATCATGC AGGCATGACT AGAGTTGACC GAACACTTGT AGAAGATCTT 
TTTGCTGACA AGCATATTCA GGTTTTAGTT TCCACCGCAA CTCTGGCGTG GGGTGTAAAT 
CTTCCTGCAC ATACAGTCAT CATTAAAGGT ACCCAAGTGT ACAGTCCAGA GAAGGGGCGT 
TGGACAGAGC TGGGAGCACT GGATATCCTG CAGATGCTGG GCCGTGCTGG ACGGCCGCAG 
TATGACACCA AGGGTGAAGG CATCCTCATC ACATCCCATG GGGAGCTCCA GTACTACCTC 
TCCCTCCTCA ACCAGCAGCT GCCTATCGAG AGCCAGATGG TCTCCAAGCT GCCTGACATG 
CTCAATGCGG AAATTGTTCT GGGCAATGTC CAGAATGCAA AGGATGCAGT GAACTGGCTG 
GGCTATGCCT ACCTATACAT CCGAATGCTC CGGTCCCCTA CCCTCTATGG CATTTCTCAT 
GATGACCTCA AGGGAGATCC CTTGCTGGAC CAGCGCCGAC TCGATCTTGT TCACACTGCT 
GCCTTGATGC TGGACAAGAA CAATCTGGTC AAGTACGACA AGAAGACAGG CAACTTCCAG 
GTGACAGAAC TTGGCCGGAT AGCAAGTCAC TACTATATCA CCAATGATAC TGTGCAGACC 
TACAACCAGC TGCTGAAGCC TACTCTGAGT GAGATTGAGC TTTTCCGAGT GTTCTCCTTG 
TCCTCAGAGT TCAAGAACAT CACTGTAAGA GAGGAGGAGA AGCTGGAGCT GCAGAAGTTG 
CTGGAGAGAG TGCCCATCCC TGTAAAGGAG AGCATTGAGG AACCCAGCGC TAAGATCAAC 
GTGCTTCTCC AAGCCTTCAT CTCACAGCTG AAACTCGAAG GCTTTGCGCT GATGGCTGAC 
ATGGTGTATG TGACCCAGTC GGCTGGCCGG TTGATGCGTG CAATCTTCGA AATTGTCCTG 
AACCGAGGTT GGGCACAGCT TACAGATAAG ACCCTGAATC TCTGCAAGAT GATTGACAAG 
CGCATGTGGC AGTCCATGTG TCCTCTTCGC CAGTTCCGAA AACTTCCTGA GGAAGTAGTG 
AAGAAGATTG AGAAGAAAAA CTTCCCCTTT GAGCGGCTGT ATGACTTGAA TCATAATGAG 
ATAGGTGAAC TTATTCGAAT GCCGAAGATG GGGAAGACCA TCCACAAGTA TGTCCATCTT 
TTCCCCAAGT TGGAGTTGTC AGTGCACCTG CAGCCTATTA CACGCTCTAC GCTGAAAGTA 
GAGCTGACTA TCACACCAGA TTTCCAGTGG GATGAAAAGG TCCATGGTTC GTCAGAGGCA 
TTTTGGATTC TGGTGGAGGA TGTGGACAGT GAGGTGATTC TGCACCATGA ATATTTTCTG 
CTGAAGGCCA AGTATGCCCA GGATGAGCAC CTCATCACGT TCTTTGTTCC AGTCTTTGAA 
CCACTACCTC CTCAGTACTT CATTCGAGTA GTGTCTGATC GCTGGCTCTC TTGTGAGACG 
CAGCTACCTG TCTCCTTCCG GCATCTGATC CTACCAGAGA AGTACCCACC TCCAACCGAA 
CTGTTGGACC TGCAGCCATT GCCTGTGTCT GCTCTGAGAA ACAGTGCTTT TGAGAGCCTT 
TACCAAGATA AATTTCCTTT CTTCAATCCC ATCCAGACTC AAGTATTTAA TACCGTGTAC 
AACAGTGATG ATAACGTGTT TGTGGGGGCC CCCACGGGCA GCGGGAAGAC TATCTGCGCG 
GAGTTTGCCA TCCTTCGGAT GCTGCTGCAG AATTCTGAGG GACGCTGTGT CTACATTACC 
CCTATGGAGG CTCTGGCAGA GCAGGTATAC ATGGACTGGT ATGAGAAGTT TCAGGACAGG 
CTCAACAAGA AGGTAGTGCT GCTGACGGGG GAGACCAGCA CAGATCTGAA GCTCCTGGGC 
AAAGGCAACA TCATCATCAG TACCCCTGAG AAGTGGGACA TCCTCTCTCG GAGGTGGAAG 
CAGCGCAAGA ACGTCCAGAA CATTAACCTC TTTGTGGTGG ATGAGGTCCA CCTTATTGGG 
GGCGAGAATG GGCCTGTCTT GGAAGTGATC TGCTCCCGGA TGCGCTACAT CTCCTCCCAG 
ATTGAGCGGC CCATTCGCAT TGTGGCACTT AGCTCCTCAC TCTCCAATGC CAAGGATGTG 
GCTCACTGGC TGGGCTGCAG TGCCACCTCC ACCTTCAACT TCCATCCTAA TGTGCGCCCT 
GTACCTTTGG AACTGCACAT CCAGGGCTTC AACATCAGTC ACACACAGAC TCGCCTGCTC 
TCTATGGCCA AGCCTGTGTA CCATGCTATC ACCAAACACT CACCCAAGAA GCCTGTCATC 
GTTTTTGTCC CATCTCGTAA GCAGACCCGC CTCACTGCAA TAGACATCCT CACTACCTGT 
GCAGCAGACA TCCAGCGGCA GAGGTTCCTG CACTGCACCG AGAAGGACCT GATCCCTTAC 
CTGGAGAAGC TCAGTGACAG CACACTCAAA GAGACCCTGT TAAATGGGGT GGGCTACCTG 
CATGAAGGCC TGAGCCCCAT GGAGAGGCGC CTGGTAGAGC AGCTCTTCAG CTCCGGGGCT 
ATCCAGGTGG TGGTAGCTTC TCGGAGTCTC TGCTGGGGCA TGAATGTTGC TGCTCATCTA 
GTGATCATCA TGGATACTCA GTACTACAAT GGCAAGATCC ATGCCTATGT GGATTACCCC 
ATCTATGATG TGCTTCAGAT GGTGGGCCAT GCCAACCGGC CCCTGCAGGA TGATGAGGGG 
CGCTGTGTCA TCATGTGTCA GGGCTCTAAA AAGGATTTTT TCAAAAAATT TTTGTATGAG 
CCATTGCCAG TAGAGTCTCA CCTGGACCAC TGTATGCATG ACCACTTCAA TGCTGAGATT 
GTCACCAAGA CCATTGAGAA CAAGCAGGAT GCTGTGGACT ACCTCACCTG GACCTTTCTG 
TATCGCAGAA TGACACAGAA CCCCAATTAC TACAACCTGC AGGGGATATC CCATCGTCAT 
CTGTCTGACC ACCTGTCAGA GCTGGTGGAG CAGACCCTCA GTGACCTGGA GCAGTCCAAA 
TGCATCAGTA TTGAGGACGA GATGGATGTG GCCCCTCTGA ACCTGGGCAT GATTGCTGCC 
TACTATTACA TAAACTACAC CACCATTGAG CTCTTCAGCA TGTCTCTGAA TGCTAAAACC 
AAGGTTCGAG GACTTATTGA GATCATTTCC AATGCAGCAG AGTATGAGAA CATTCCAATC 
AGGCATCATG AAGACAACCT CCTGCGCCAG TTGGCTCAGA AGGTCCCCCA CAAGCTGAAT 
AACCCCAAGT TCAATGATCC ACATGTGAAG ACCAATCTGC TGCTGCAGGC TCACCTGTCC 
CGCATGCAGC TAAGTGCTGA ACTACAGTCA GACACAGAGG AGATCCTTAG TAAGGCAATC 
CGGCTAATTC AGGCCTGTGT GGATGTACTC TCCAGCAATG GGTGGCTTAG TCCTGCTCTG 
GCAGCCATGG AACTGGCCCA GATGGTCACC CAAGCCATGT GGTCTAAGGA CTCTTACCTG 
AAGCAGCTGC CACACTTCAC CTCAGAGCAT ATCAAACGTT GCACAGATAA GGGAGTGGAG 
AGTGTTTTTG ACATCATGGA GATGGAGGAT GAAGAACGGA ATGCATTGCT TCAGTTGACT 
GACAGCCAGA TTGCAGATGT GGCCCGCTTC TGTAACCGCT ACCCGAATAT TGAACTGTCC 
TATGAAGTGG TGGATAAAGA CAGCATCCGC AGTGGCGGAC CAGTTGTGGT GCTAGTGCAA 
CTGGAGCGAG AGGAGGAAGT CACGGGCCCA GTTATTGCAC CTCTCTTCCC ACAGAAACGT 
GAAGAGGGCT GGTGGGTTGT GATTGGAGAC GCCAAGTCCA ACAGCCTCAT CTCCATCAAG 
AGGCTGACCC TGCAGCAGAA AGCCAAGGTG AAGCTAGACT TTGTGGCCCC AGCCACAGGT 
GGCCACAACT ATACCCTGTA CTTCATGAGT GACGCATACA TGGGCTGTGA CCAGGAGTAT 
AAGTTCAGTG TGGATGTGAA AGAAGCTGAG ACAGACAGTG ATTCAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796188.2
CDS	109..6519
Translation	MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTKPQMQEERRAKRRKRDEDRHDMNKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFIQAALGDQPRDILCGAADEVLAVLKNEKLRDKERRREIDLLLGQTDDTRYHVLVNLGKKITDYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTLSANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEPEKERIVGKMEADPELSKFLYQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIVLDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQNSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGGHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD

Target ORF information:

RefSeq Version	NM_177214.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus small nuclear ribonucleoprotein 200 (U5) (Snrnp200), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177214.5

ORF Insert Sequence:

ATGGCGGATG TGACTGCCCG CAGTTTGCAG TACGAGTACA AGGCGAACTC AAATCTTGTG 
CTCCAAGCTG ACCGCTCTCT CATTGACCGG ACCCGCCGGG ATGAACCGAC GGGAGAGGTT 
CTGTCCCTGG TTGGGAAGTT GGAGGGAACC CGAATGGGAG ATAAGGCTCA ACGGACCAAA 
CCACAGATGC AGGAAGAAAG ACGGGCCAAG AGGAGAAAAC GTGATGAGGA CCGGCATGAC 
ATGAACAAGA TGAAAGGCTA CACGCTGCTG TCTGAGGGCA TTGATGAGAT GGTGGGCATC 
ATCTACAAGC CTAAGACGAA GGAGACCCGA GAGACCTACG AGGTGCTGCT CAGCTTCATC 
CAGGCTGCGC TGGGAGATCA GCCTCGAGAT ATTCTTTGTG GAGCAGCTGA TGAAGTCCTG 
GCTGTGCTGA AGAATGAAAA GCTTCGAGAC AAGGAGAGAA GACGGGAGAT TGACCTGCTG 
CTGGGTCAGA CAGATGATAC CAGATACCAT GTGCTGGTAA ACCTGGGCAA GAAGATCACA 
GACTACGGTG GCGACAAGGA GATACAGAAT ATGGATGACA ACATTGATGA GACTTATGGT 
GTGAACGTGC AGTTTGAGTC TGATGAGGAG GAAGGTGATG AAGATGTGTA TGGGGAAGTC 
CGAGAAGAAG CTTCTGATGA TGACATGGAA GGGGACGAAG CTGTTGTGCG CTGCACCCTC 
TCTGCTAATC TTGTAGCCTC AGGCGAACTG ATGAGTTCTA AGAAGAAGGA TTTGCACCCT 
CGGGATATCG ATGCCTTCTG GCTGCAGCGA CAGCTCAGTC GTTTCTATGA TGATGCCATT 
GTGTCACAGA AGAAGGCAGA TGAGGTGTTG GAGATTTTGA AGACAGCAAG TGATGACCGA 
GAATGTGAGA ACCAACTGGT TTTGCTCCTT GGTTTCAACA CGTTTGACTT CATTAAAGTG 
TTGCGGCAGC ACAGGATGAT GATTTTATAC TGTACCTTGT TGGCCAGTGC TCAGAGTGAA 
CCTGAAAAGG AGAGGATCGT GGGAAAGATG GAAGCTGATC CAGAGCTCTC CAAGTTCCTC 
TATCAGCTCC ATGAGACTGA GAAGGAGGAC CTGATCAGAG AAGAAAGGTC CCGGAGAGAG 
AGAGTCCGTC AGTCCCGAAT GGACACAGAT CTGGAAACCA TGGATTTGGA CCAGGGTGGA 
GAGGCTCTGG CTCCAAGGCA GGTTCTGGAT TTGGAGGACC TTGTTTTTAC CCAAGGAAGC 
CACTTCATGG CCAATAAGCG CTGCCAGCTT CCTGACGGGT CCTTCCGACG CCAGCGTAAA 
GGCTATGAAG AGGTGCATGT GCCTGCTTTG AAGCCCAAGC CCTTTGGCTC AGAAGAACAA 
TTGCTCCCGG TGGAGAAGCT GCCAAAGTAT GCCCAGGCAG GATTTGAGGG CTTCAAAACG 
CTGAACCGGA TCCAGAGTAA GCTCTACCGT GCTGCCCTGG AGACTGATGA GAACCTGCTG 
CTGTGTGCTC CCACTGGTGC GGGCAAGACC AACGTGGCCC TGATGTGTAT GCTCCGAGAG 
ATAGGGAAAC ACATCAACAT GGACGGCACA ATCAATGTGG ATGACTTCAA GATCATCTAC 
ATAGCTCCCA TGCGGTCCCT GGTCCAGGAG ATGGTGGGCA GTTTTGGAAA GCGCCTGGCC 
ACATATGGCA TCACTGTTGC TGAGCTGACT GGGGATCACC AGCTATGCAA GGAGGAAATC 
AGTGCCACAC AGATTATCGT CTGCACCCCT GAGAAGTGGG ACATCATCAC ACGCAAGGGC 
GGGGAGCGCA CCTACACCCA GCTGGTGCGA CTCATTGTCT TGGATGAGAT CCATCTTCTA 
CATGATGACA GAGGTCCTGT CTTAGAAGCT TTGGTGGCCA GGGCCATCCG AAACATTGAG 
ATGACCCAAG AAGATGTCCG ACTCATTGGT CTCAGTGCTA CCCTCCCCAA CTATGAAGAT 
GTGGCCACCT TTCTGCGAGT CGACCCTGCT AAGGGCCTCT TCTACTTTGA TAACAGCTTC 
CGCCCCGTGC CTCTGGAACA AACATATGTG GGCATCACAG AGAAAAAAGC TATCAAACGT 
TTCCAGATCA TGAATGAAAT AGTCTATGAG AAAATCATGG AACATGCTGG AAAAAATCAG 
GTGCTCGTGT TTGTCCATTC TCGAAAAGAA ACTGGGAAGA CAGCAAGGGC AATCCGTGAC 
ATGTGTCTGG AGAAGGACAC TTTGGGTCTG TTTCTTCGCG AGGGTTCTGC CTCCACTGAA 
GTCCTTCGTA CAGAAGCAGA GCAGTGCAAG AACTTGGAGC TGAAGGATCT TTTGCCCTAT 
GGCTTTGCTA TTCATCATGC AGGCATGACT AGAGTTGACC GAACACTTGT AGAAGATCTT 
TTTGCTGACA AGCATATTCA GGTTTTAGTT TCCACCGCAA CTCTGGCGTG GGGTGTAAAT 
CTTCCTGCAC ATACAGTCAT CATTAAAGGT ACCCAAGTGT ACAGTCCAGA GAAGGGGCGT 
TGGACAGAGC TGGGAGCACT GGATATCCTG CAGATGCTGG GCCGTGCTGG ACGGCCGCAG 
TATGACACCA AGGGTGAAGG CATCCTCATC ACATCCCATG GGGAGCTCCA GTACTACCTC 
TCCCTCCTCA ACCAGCAGCT GCCTATCGAG AGCCAGATGG TCTCCAAGCT GCCTGACATG 
CTCAATGCGG AAATTGTTCT GGGCAATGTC CAGAATGCAA AGGATGCAGT GAACTGGCTG 
GGCTATGCCT ACCTATACAT CCGAATGCTC CGGTCCCCTA CCCTCTATGG CATTTCTCAT 
GATGACCTCA AGGGAGATCC CTTGCTGGAC CAGCGCCGAC TCGATCTTGT TCACACTGCT 
GCCTTGATGC TGGACAAGAA CAATCTGGTC AAGTACGACA AGAAGACAGG CAACTTCCAG 
GTGACAGAAC TTGGCCGGAT AGCAAGTCAC TACTATATCA CCAATGATAC TGTGCAGACC 
TACAACCAGC TGCTGAAGCC TACTCTGAGT GAGATTGAGC TTTTCCGAGT GTTCTCCTTG 
TCCTCAGAGT TCAAGAACAT CACTGTAAGA GAGGAGGAGA AGCTGGAGCT GCAGAAGTTG 
CTGGAGAGAG TGCCCATCCC TGTAAAGGAG AGCATTGAGG AACCCAGCGC TAAGATCAAC 
GTGCTTCTCC AAGCCTTCAT CTCACAGCTG AAACTCGAAG GCTTTGCGCT GATGGCTGAC 
ATGGTGTATG TGACCCAGTC GGCTGGCCGG TTGATGCGTG CAATCTTCGA AATTGTCCTG 
AACCGAGGTT GGGCACAGCT TACAGATAAG ACCCTGAATC TCTGCAAGAT GATTGACAAG 
CGCATGTGGC AGTCCATGTG TCCTCTTCGC CAGTTCCGAA AACTTCCTGA GGAAGTAGTG 
AAGAAGATTG AGAAGAAAAA CTTCCCCTTT GAGCGGCTGT ATGACTTGAA TCATAATGAG 
ATAGGTGAAC TTATTCGAAT GCCGAAGATG GGGAAGACCA TCCACAAGTA TGTCCATCTT 
TTCCCCAAGT TGGAGTTGTC AGTGCACCTG CAGCCTATTA CACGCTCTAC GCTGAAAGTA 
GAGCTGACTA TCACACCAGA TTTCCAGTGG GATGAAAAGG TCCATGGTTC GTCAGAGGCA 
TTTTGGATTC TGGTGGAGGA TGTGGACAGT GAGGTGATTC TGCACCATGA ATATTTTCTG 
CTGAAGGCCA AGTATGCCCA GGATGAGCAC CTCATCACGT TCTTTGTTCC AGTCTTTGAA 
CCACTACCTC CTCAGTACTT CATTCGAGTA GTGTCTGATC GCTGGCTCTC TTGTGAGACG 
CAGCTACCTG TCTCCTTCCG GCATCTGATC CTACCAGAGA AGTACCCACC TCCAACCGAA 
CTGTTGGACC TGCAGCCATT GCCTGTGTCT GCTCTGAGAA ACAGTGCTTT TGAGAGCCTT 
TACCAAGATA AATTTCCTTT CTTCAATCCC ATCCAGACTC AAGTATTTAA TACCGTGTAC 
AACAGTGATG ATAACGTGTT TGTGGGGGCC CCCACGGGCA GCGGGAAGAC TATCTGCGCG 
GAGTTTGCCA TCCTTCGGAT GCTGCTGCAG AATTCTGAGG GACGCTGTGT CTACATTACC 
CCTATGGAGG CTCTGGCAGA GCAGGTATAC ATGGACTGGT ATGAGAAGTT TCAGGACAGG 
CTCAACAAGA AGGTAGTGCT GCTGACGGGG GAGACCAGCA CAGATCTGAA GCTCCTGGGC 
AAAGGCAACA TCATCATCAG TACCCCTGAG AAGTGGGACA TCCTCTCTCG GAGGTGGAAG 
CAGCGCAAGA ACGTCCAGAA CATTAACCTC TTTGTGGTGG ATGAGGTCCA CCTTATTGGG 
GGCGAGAATG GGCCTGTCTT GGAAGTGATC TGCTCCCGGA TGCGCTACAT CTCCTCCCAG 
ATTGAGCGGC CCATTCGCAT TGTGGCACTT AGCTCCTCAC TCTCCAATGC CAAGGATGTG 
GCTCACTGGC TGGGCTGCAG TGCCACCTCC ACCTTCAACT TCCATCCTAA TGTGCGCCCT 
GTACCTTTGG AACTGCACAT CCAGGGCTTC AACATCAGTC ACACACAGAC TCGCCTGCTC 
TCTATGGCCA AGCCTGTGTA CCATGCTATC ACCAAACACT CACCCAAGAA GCCTGTCATC 
GTTTTTGTCC CATCTCGTAA GCAGACCCGC CTCACTGCAA TAGACATCCT CACTACCTGT 
GCAGCAGACA TCCAGCGGCA GAGGTTCCTG CACTGCACCG AGAAGGACCT GATCCCTTAC 
CTGGAGAAGC TCAGTGACAG CACACTCAAA GAGACCCTGT TAAATGGGGT GGGCTACCTG 
CATGAAGGCC TGAGCCCCAT GGAGAGGCGC CTGGTAGAGC AGCTCTTCAG CTCCGGGGCT 
ATCCAGGTGG TGGTAGCTTC TCGGAGTCTC TGCTGGGGCA TGAATGTTGC TGCTCATCTA 
GTGATCATCA TGGATACTCA GTACTACAAT GGCAAGATCC ATGCCTATGT GGATTACCCC 
ATCTATGATG TGCTTCAGAT GGTGGGCCAT GCCAACCGGC CCCTGCAGGA TGATGAGGGG 
CGCTGTGTCA TCATGTGTCA GGGCTCTAAA AAGGATTTTT TCAAAAAATT TTTGTATGAG 
CCATTGCCAG TAGAGTCTCA CCTGGACCAC TGTATGCATG ACCACTTCAA TGCTGAGATT 
GTCACCAAGA CCATTGAGAA CAAGCAGGAT GCTGTGGACT ACCTCACCTG GACCTTTCTG 
TATCGCAGAA TGACACAGAA CCCCAATTAC TACAACCTGC AGGGGATATC CCATCGTCAT 
CTGTCTGACC ACCTGTCAGA GCTGGTGGAG CAGACCCTCA GTGACCTGGA GCAGTCCAAA 
TGCATCAGTA TTGAGGACGA GATGGATGTG GCCCCTCTGA ACCTGGGCAT GATTGCTGCC 
TACTATTACA TAAACTACAC CACCATTGAG CTCTTCAGCA TGTCTCTGAA TGCTAAAACC 
AAGGTTCGAG GACTTATTGA GATCATTTCC AATGCAGCAG AGTATGAGAA CATTCCAATC 
AGGCATCATG AAGACAACCT CCTGCGCCAG TTGGCTCAGA AGGTCCCCCA CAAGCTGAAT 
AACCCCAAGT TCAATGATCC ACATGTGAAG ACCAATCTGC TGCTGCAGGC TCACCTGTCC 
CGCATGCAGC TAAGTGCTGA ACTACAGTCA GACACAGAGG AGATCCTTAG TAAGGCAATC 
CGGCTAATTC AGGCCTGTGT GGATGTACTC TCCAGCAATG GGTGGCTTAG TCCTGCTCTG 
GCAGCCATGG AACTGGCCCA GATGGTCACC CAAGCCATGT GGTCTAAGGA CTCTTACCTG 
AAGCAGCTGC CACACTTCAC CTCAGAGCAT ATCAAACGTT GCACAGATAA GGGAGTGGAG 
AGTGTTTTTG ACATCATGGA GATGGAGGAT GAAGAACGGA ATGCATTGCT TCAGTTGACT 
GACAGCCAGA TTGCAGATGT GGCCCGCTTC TGTAACCGCT ACCCGAATAT TGAACTGTCC 
TATGAAGTGG TGGATAAAGA CAGCATCCGC AGTGGCGGAC CAGTTGTGGT GCTAGTGCAA 
CTGGAGCGAG AGGAGGAAGT CACGGGCCCA GTTATTGCAC CTCTCTTCCC ACAGAAACGT 
GAAGAGGGCT GGTGGGTTGT GATTGGAGAC GCCAAGTCCA ACAGCCTCAT CTCCATCAAG 
AGGCTGACCC TGCAGCAGAA AGCCAAGGTG AAGCTAGACT TTGTGGCCCC AGCCACAGGT 
GGCCACAACT ATACCCTGTA CTTCATGAGT GACGCATACA TGGGCTGTGA CCAGGAGTAT 
AAGTTCAGTG TGGATGTGAA AGAAGCTGAG ACAGACAGTG ATTCAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
BMC developmental biology514(2005 Jul)
McKee AE,Minet E,Stern C,Riahi S,Stiles CD,Silver PA

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)
Okazaki N,F-Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Seino S,Nishimura M,Kaisho T,Hoshino K,Kitamura H,Nagase T,Ohara O,Koga H

Comparative sequence analysis of a single-gene conserved segment in mouse and human.
Mammalian genome : official journal of the International Mammalian Genome Society14(10)673-8(2003 Oct)
Thomas JW,,Green ED

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Snrnp200 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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