AgaP_AGAP002836 cDNA ORF clone, Anopheles gambiae str. PEST

Gene
Clones
gRNAs

Summary

Gene Symbol	AgaP_AGAP002836
Entrez Gene ID	1273124
Full Name	AGAP002836-PB
Synonyms	AgaP_ENSANGG00000014308,ENSANGG00000014308
Gene Type	protein-coding
Organism	Anopheles gambiae str. PEST

mRNA and Protein(s)

mRNA	Protein	Name
XM_003436208.1	XP_003436256.1	AGAP002836-PB
XM_312076.3	XP_312076.2	AGAP002836-PA

Pathways from BioSystems

Homologies

Homo sapiens (human)	DICER1	NP_085124.2
Rattus norvegicus (Norway rat)	Dicer1	XP_006240573.1
Danio rerio (zebrafish)	dicer1	NP_001154925.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002836	XP_003436256.1
Magnaporthe oryzae (rice blast fungus)	MGG_01541	XP_003714515.1
Neurospora crassa	NCU08270	XP_961898.1
	DICER1	XP_001100868.1
Gallus gallus (chicken)	DICER1	NP_001035555.1
Drosophila melanogaster (fruit fly)	Dcr-1	NP_524453.1
Caenorhabditis elegans (roundworm)	dcr-1	NP_498761.2
Xenopus tropicalis (tropical clawed frog)	dicer1	NP_001123390.2
Canis lupus familiaris (dog)	DICER1	XP_868526.3
Bos taurus (cattle)	DICER1	NP_976235.1
Schizosaccharomyces pombe (fission yeast)	dcr1	NP_588215.2
Pan troglodytes (chimpanzee)	DICER1	XP_001154369.1
Mus musculus (house mouse)	Dicer1	NP_683750.2

Gene Ontology
Bibliography

Related articles in PubMed

The Anopheles gambiae genome: an update.
Mongin E, Louis C, Holt RA, Birney E, Collins FH
Trends in parasitology20(2)49-52(2004 Feb)

The genome sequence of the malaria mosquito Anopheles gambiae.
Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, Nusskern DR, Wincker P, Clark AG, Ribeiro JM, Wides R, Salzberg SL, Loftus B, Yandell M, Majoros WH, Rusch DB, Lai Z, Kraft CL, Abril JF, Anthouard V, Arensburger P, Atkinson PW, Baden H, de Berardinis V, Baldwin D, Benes V, Biedler J, Blass C, Bolanos R, Boscus D, Barnstead M, Cai S, Center A, Chaturverdi K, Christophides GK, Chrystal MA, Clamp M, Cravchik A, Curwen V, Dana A, Delcher A, Dew I, Evans CA, Flanigan M, Grundschober-Freimoser A, Friedli L, Gu Z, Guan P, Guigo R, Hillenmeyer ME, Hladun SL, Hogan JR, Hong YS, Hoover J, Jaillon O, Ke Z, Kodira C, Kokoza E, Koutsos A, Letunic I, Levitsky A, Liang Y, Lin JJ, Lobo NF, Lopez JR, Malek JA, McIntosh TC, Meister S, Miller J, Mobarry C, Mongin E, Murphy SD, O'Brochta DA, Pfannkoch C, Qi R, Regier MA, Remington K, Shao H, Sharakhova MV, Sitter CD, Shetty J, Smith TJ, Strong R, Sun J, Thomasova D, Ton LQ, Topalis P, Tu Z, Unger MF, Walenz B, Wang A, Wang J, Wang M, Wang X, Woodford KJ, Wortman JR, Wu M, Yao A, Zdobnov EM, Zhang H, Zhao Q, Zhao S, Zhu SC, Zhimulev I, Coluzzi M, della Torre A, Roth CW, Louis C, Kalush F, Mural RJ, Myers EW, Adams MD, Smith HO, Broder S, Gardner MJ, Fraser CM, Birney E, Bork P, Brey PT, Venter JC, Weissenbach J, Kafatos FC, Collins FH, Hoffman SL
Science (New York, N.Y.)298(5591)129-49(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AgaP_AGAP002836 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AgaP_AGAP002836 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAh02780	XM_003436208.1 Latest version!	Anopheles gambiae str. PEST AGAP002836-RB (AgaP_AGAP002836), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OAh02781	XM_312076.3 Latest version!	Anopheles gambiae str. PEST AGAP002836-RA (AgaP_AGAP002836), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AgaP_AGAP002836 ( XM_003436208.1 ) cDNA ORF clone, Anopheles gambiae str. PEST -> XP_003436256.1 Anopheles gambiae str. PEST AGAP002836-RB (AgaP_AGAP002836), partial mRNA.

Price & Availability↑

CloneID	OAh02780
Clone ID Related Accession (Same CDS sequence)	XM_003436208.1
Accession Version	XM_003436208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-25
Organism	Anopheles gambiae str. PEST
Product	AGAP002836-PB
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_078266). COMPLETENESS: incomplete on both ends.

ATGTCGTTGT TCCACTGGAC GGATGGCAAC ATCCACACCA CAGCCCTCAC GCCCCGGGAC 
TATCAAACCG AGCTGCTGGC TACGGCTCGG GAAGAAAATC TTATCGTCTG TATAGCGCAC 
AATTCCGCCA AAGAGTTCCT GGCCGTAAAG TTGATCCAGT CGATGCGCAC CAATCGCTGG 
AGCAGTCACC CTGAAGCACC GGGAAAAGCC ATCTACCTCA CGCGAATGGA TCGAAGCTTG 
CTGTCGTCGA TGGTTTCGAA TTTGACCGAC CTACAGGTCG CCAACGTCGA TGATGTGGAG 
GACAGTGAAG GGTCACACGA ACCGGACGGT GCTTCCAACA CGCCGGTGAC GGATGTTGCT 
TCGGCCGATG TGCTGTTCTT CGGCAGTGAA ACAACATTGC TGCAGTACAT CGAGCAAGGT 
ACGGTACGGG TGCAAGACAT AAGCCTGTTG ATTGTGGACG AATGCCACAA AAACTACGGT 
CGACAGGAAC TGTGGGAGAT CTGTGCTCGA CTGACGCATC AGGCACCGTC GTCCGATAGG 
CCCGCTCAAC GGACGAGAAT CTTGGGATTA GCTGGTCCAT TGCATGGTGC GGGCTGTACA 
CCGGAACGGT TGTGCTGGGA GCTGCATTAC CTTGAACGAT GCTTGCGTGC ACGTATCGAA 
ACTGCGAGCG ACATCACAAG CGTTCTCAGA TTCAGCACAA AACCTACGGA ACTAATCCTG 
GAATGCATCC CACCCAAGCC ATCCAATTTG ACGCAGCTTC TTAGGATGTT AATACAGCGT 
CAGATCGCTT TCCTGAAGCA GCATCGCTAT GAACCGCTAG CCGTGTACGG ACTGGACGGT 
AACGATAGCG CTAGTGATAA GCCGGACACA GACGGGGAAC CGGAGAAGGA TGAAGAAAAC 
GATGACCTGC GCCGTGAGCT AAAATCCATC CCCGATCCCA CGGTCGGTCC GTTGAGCTAC 
CTAAAGCAGT ATCTCGAGCT GCTGGACGAG TTCGGACCCT GGGGCGCTGA TCGCGGTGCA 
CTCGAACTTC TAACGACCAT CGACCAGGAG AAGGTGAAAG TGCCTTACGA CCGGCACTTT 
CTTCTGTTCT GCATGGTGTA CACCACGCTG CTGCAGGCAA GAGCAACGGT GGCGTCCGTC 
TTCGCACAGC ACGACACCGA ACTGGAGCGC ATCAAGCGCT ATTCGACGCC CAAGGTTCGC 
CGCCTGCTCG AGGTGCTGGC ATGGTTCGGT GAACAACGCA ACCGGCCGAA AGATCGCAAC 
CAACCGGCCA CACTGCACCA TCATCAGCAG GTGCACAATC AGCAGCGCAT ATTGTACTGC 
TTCTGCCGCA ACGTCGAGTG TAAAGAGCTG GAGAAAAGCT ACCACACTTT CGGCGCCCAA 
ATAGCGGACG TTGATGAGCG GATCAAGCAG CTGGACGGTT TGCTAGCATC CGTGCGCAAG 
CGAACCGAAC GGTTGAACCT AAAGCACAAA GTCGTCGACA ATGCGGCCGC CGAGGGTGGT 
GGTACGCTAG GGGTGCAGTC ACCCCGGCAC GGTCACGAGA GCCGAGCAAA CAATTTCCGC 
CGCAAGCGAT TCGCGGGCGG GGGACATTCG CACCACCGAT CGAACGACAC GACCGATGCG 
CTGTGCGGGT TAATTTTCTG CAACAACCGG GCGATGGCCC GCATTCTTTA CGTGCTGCTG 
TACGAGGTGT CTCGTTCGCA GCGCGAATTT GAGTTCATCA GCCCACAGTA CACGGTGGAC 
AAGGTGGCCA CCAATCCGCA AAACTGCCTC AAGCAGACCA CCATCGAGCA TCGCAAGCAG 
GAGGAGGTGC TGAAACGCTT CCGAATGCAC GAATGCAACC TGTTGATTGG GACGTCCGTG 
TTGGAGGAAG GCATCGAGCT GCCAAAGTGT AACCTAGTTA TTCGCTGGAA CAGTCCCGCC 
AACTATCGGT CCTACGCTCA GTGCAAGGGT CGAGCGAAAG CGCCGGGCGC GTACCATGTG 
TTGTTTGTCA CGCCCGAAAA TGCTGCCTCG CGCAACGAAC AACAGGAGGA TATGGCATCG 
ATCGAGGATG TTTCACTTGA TGGCATGATT CCGGAAGGGC CGAAAGACGA CAATACGCGC 
GAAACGATCG ATGATCAGGA CCGAAGAATG ATCGAAACAG CAACAGATGC GATGATCGAG 
CAGGTTGCCA TCTACCGTGA AGTTGAAAAG CTTTTACTTG CCAAGTGCCG CAATGGAGAG 
CCGCCCGATT GGGAGCTGAA GCATGCCGAC TGCTTCAACC ACTGCCTAGA AGTGTATCGT 
CCGAGTAGTG GTGGTGCCGT TGCACTTCAA TCCAACGGAA CGTGCGCCTC ACTGTGGCTG 
GGAAATGCCA TCCAAACACT CAACAAGTAC TGTGCCAAGC TGCCGAGCGA TACGTTCACT 
AAGCTTACTC CGATTTGGCG CTGTGCCACT ACCGTCCGGA AGGGGCGCAA GCTGTACCAA 
TACACCATTC GCCTGCCCAT AAACTCGCCC TGGAAAGAAG ACATATTGGG ATTGCCGATG 
CCTACGGAAA CGCTAGCACG CAGGCTGGCT GCCTATATGA CCTGCCGGAT GCTGCATGCC 
GCTGGGGAGC TTGACAATTC CTTTCAACCG TTCGGCAAGG AAGCCTTTCG TGCGTTCGAA 
GCGGACTGGG AAAACTTTGA GCTGGAGGAA TCGGACGCAA AAATACTGTC CGAAAACAGC 
GACCCCAGAC CGGGCACAAC AAAACGGCGG CAATATTACT ACAAAAGGAT TGCTTCCGTG 
TTCAACGAGT GTCGACCGGA CGCTGAAACC GTCGCCTATC TCTACCACAT ACGCATGGAG 
CTGATCTGCC CCATCCCCGA GGAGCAGAAT ACGCGCGGGC GCAAAATATA CGCACCGGAA 
GAATCCGCAC AAGGGTTTGG CATTCTGACC ACGAAGCTGA TACCAAAGAT AAGCTCCTTT 
CCCATATTCA CCCGGTCCGG CGAGGTGAAA GTGTCGCTCG ATCTGTGCCC GCAGCGCGTT 
AAGCTGTCCG CCCACCAGCT GGAGATGGTA AACTGTTTCG TGAAGTACAC GTTCACCAAA 
GTGTTGCGCT TGCAGAAGAG CCTGATGCTG TACGATGCGA ACGCAACGGA GAACTGTTTC 
TTCATCGTTC CGACGGTCAA ACAAGCTGTT GCGGGTGCGG CTGGTAAAGA CGAACCACCG 
CTCCAATCAG ACGACGTGAT GGTGGACTGG GAGTTTGTGG AAAAGATTGC CACGAACGTG 
CATCGCAGCG GACCAACATT CATACCGGAT GAGGCACGCA AGGGCTACAC GTTCGATGTG 
GGCAAATTTC GGGACGCCGT TGTAATGCCG TGGTATCGGA ACCGCGACCA GCCGCAGTAC 
TTTTACGTGG CGGAAATATG CAACCATCTG TCGCCGAAGA GCACCTTTCC CGGGTCGAAC 
TACGCGACGT TCGAGGAGTA TTACCATCGC AAGTACAAAA TCCACATTCA GAACCAACGC 
CAGCCGCTGC TGGACGTTGA CCATACCAGT GCGCGGCTAA ACTTCCTGAC GCCCCGTTAC 
GTTAACCGGA AGGGTGTAGC GTTGCCGACC AGCTCGGAGG AGACGAAGCG GGCCAAGCGT 
GAAAACTTGG AGCAGAAACA GATCCTCGTG CCGGAGCTGT GCACGATACA TCCGTTCCCT 
GCCTCGCTGT GGCGTGCGGC CGTCTGCCTG CCGTGCGTAC TGTACCGCAT CAACGCGCTG 
CTGCTGGCAG ATGAGATACG GCGGCAGGTG GCACGCGACC TGCGGCTGGG ATGGGAAAAT 
GTGGACGAGC TGCAGGAGGG CCAGTTCCAG TGGCCAATGC TAAGCTTTGG CTGGAATCTG 
GCCGATGTGC TGCGCAAAAC GAAGGAGCAG AAAATTGCGC AAGCTCAGGA AGCGATCGAT 
GCCAGTGCGC CGGAAGTGGA GGATGAAGTG GAGCTAGACA AGGAAGCGCC GAACGTGCGT 
GATGCTGCGG AGGTTGATGA GGAAGATGGG CTAAAAATGG AAAACGGTGT AATTGCTGAG 
GTTGAAAAGA GTCAGGTGGA TGGAGAGGAT GATACTGGCG ATAAAAAGAC TGATAGCGAT 
GGAACGCTGC TTGAAATTGG TACATGGTCG AACGAAATGG CGGTAGGCGT TGGAACCGAT 
AACGATATGG GTGAAGAAGG TAGGAGAGGC GCATCGTCAC CCTCGTTCCT GCGGTACGAT 
TCCGACTGTT CCAGCAACAG CAGTGCCAAT TTCTACTCTT CGGACGAGTA CGACGAGGAG 
GACGATTACT ACCTGTACGA TGGTTCGGAA AAGCCGAAAA ATGCGATAGA ACCGAGCCAG 
GAGGCAGTAT CGGGCACGGA CAATGCGAAC GACTCGGGCG ATAAGCCGGG CAGTAGGAAC 
CGGACCATCA CGCAGTCACA GGACACGGTG GTAAATCTGG GTGGGCGGTT GAAGATCGAG 
TTCAAGTCGG AAACCGATGC CGAAGCGATC GATTCCGAGT GTGATCTGCA GCGGCAGCGA 
ACGCAGCAGA GCATCATTGA GCGGTCACGC CAGAACGATA TGCTCTATCA GAGCTCGAAA 
AACGCGGTCG ATGGGTTCTG CTACTCGCCG AGCGATCGGC AGTGCGCCGA GGAGCGGGAG 
CAAGCGGAGC AACGGTTTGA GCGGCAGAAA AATCACACCA AAGACACCAT CCGCCAGCAG 
GGCAGCTTGG TGCGATGGAA CGAACCGCTG TCCGTAAGCC ACTGGCGATC GAAGCTGGAC 
GAGTCGGAAG CGGCCACCCT TGGCGCACTG ATGGACGATT TGGGCGGGCA ACCGTTCACG 
GAGCTCGTAC CGTACGTCGA GCCGGAGCAG CTGTTCGAGC TGCTTGTGCG AAACGGTAGC 
AGCGGGGAAC GATGGCTCCG ATTGCACGAT CTCTACTGCT TGAATCGACC GTTCTTCCCG 
GAACGGTACA CCATCTTCCG GGGCGGTTCA CAGTTTGATA ACTTTCTGGA CGAAAGCTGC 
CAGGAAGGTG AGGGTGCTAG TTCGCCACCG AAAGTGATCG AGCTGACTAT TTGCGATCCT 
TTTCCGGCGA TAGCCGCCGG GCAGATGGCT TGCAACTACA AACCGAAACA ACATTCCATG 
CGGAGCGACG AAGCGAACAA GAACGGCCAC GACAGTGCGG GTGTGCTCAG TACGACAGAT 
GAGGCCGGGG GTGTTGATTA CTTCAGTTTC GATTATCAGC CAGATTTGAG CCAACATCCC 
GGTCCCAGTC CGGCTATTAT TCTACAAGCG CTAACCATGT CGAACGCCAA CGATGGCATC 
AACCTGGAGC GATTGGAAAC GATTGGCGAT TCCTTTCTGA AGTACGCCAT CACCACCTAC 
CTTTACTGCC GGTACGACAA CGTGCACGAG GGCAAGCTAA GCCACCTGCG GTCGAAGCAG 
GTATCCAATC TGAACCTTTA CCGGTTGGGG CGCAGGAAGC GGTTGGGCGA CTGCATGATT 
GCGGCCAAAT TTGAACCACA CGATAACTGG CTGCCACCCT GCTACTACGT GCCGAAGGAG 
CTAGAACAAA CGTTGATTGA TGCAAAGATC CCTGCTTGCC ATTGGAATCT GGCCGATCTT 
CCAGACATCA AGCGTCTGTC GTGCGCTGAA ATCTGTCAAC TAGTCAAGGA ACGTGCACGG 
GCCAAACGCA GAGAGGATGT CGACCGGTTC GACCTACAAC ATGCAGACAG CAACGGTGGT 
GAGGACGGAA ATGAAGACGA CGACGATGAC GACGACGACG ATGAAGACGA TACAACCACT 
GGCGAAGGGA ACGAGCACGA AGCGGACAAT GGGTCCTGCT ACATCCCGTA CAATTTAGTA 
ACGCAGCACA GCATCCCCGA CAAATCGGTG GCTGACTGTG TGGAAGCCCT GATCGGTGCC 
TACCTCATCG AATGTGGCCC CCGCGGAGCG CTCCTCTTTA TGGCATGGCT TGGCATACGG 
GTGCTCCCCA TCCGTGAGCC GCCGGTGAAG CTTAATTCAA ACAACGAGAC GGCGCTTACT 
CCTTACAAAG CTACTGGACA GAACGATGGA CCCCTGTCCA CTGGCGTAAC GATTGCTGAG 
TATGGTCACT GGGTTGCACC GCCCTCGCCC ATGGTGCGGG CCAACATTAC GTTCGGTGGC 
ATCGAAACCG GTGCCGCGGC AACGTCCCGC GAGCTGGCCC GGCTGCTGCA AGGGTTCGAG 
GAGTTCGAGC AGGCGCTTGG CTACCGCTTC CGGGACCGTT CCTACCTGCT GCAAGCCATG 
ACGCACGCTT CGTACAGCCC GAACCGGCTG ACGGATTGCT ACCAGCGGCT GGAGTTTCTG 
GGCGATGCCA TCCTCGACTA CCTCATCACG CGCCATCTGT ACGAGGACCG CCGGCAGCAT 
TCACCGGGCG CGCTCACAGA CTTGCGGTCG GCGCTGGTGA ACAATACCAT CTTTGCTTCG 
CTCGCCGTAC GCCACGGATT TCATAAGTAC TTCCTGCACC TGTCGCCCGG GCTGCAGGAG 
GTGATCGATC GATTCGTTCG CATCCAGCAA GAGAACGGGC ATCGCATCAC GGAGGAGGAA 
TACTACCTGC CGGACGAAGA TGACGAGCTG GGCGAGTACG GTGCGATGGG GGAGGACGGT 
CCCGGCGAGG GCCGCGGTGT GGGGGAGGCG GAAGATGTGG AAGTGCCGAA AGCGTTGGGC 
GACGTGTTCG AATCGATTGC CGGTGCCATT TTCCTCGATT CCGACATGTC GCTCGATACG 
GTGTGGAAGG TGTATCGGAA AATGATGGGA CCGGAAATAG AGAAATTCAG CAGCTCGGTC 
CCAAAATCCC CGATACGCGA ACTGCTCGAA ATGGAACCGG AGACGGCCAA ATTTGGCAAA 
CCGGAGAAGC TGACGGACGG ACGGCGCGTG CGGGTAACGG TGGAAGTGTT CGGCAAAGGT 
ACATTCCGTG GCATTGGAAG GAACTATCGC ATCGCCAAGT GTACGGCGGC AAAGTGTGCC 
CTTCGCCAGC TCAAGAAGCT CGGCTATGCC AACCATCACA AGCGCCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003436256.1
CDS	1..7011
Translation	MSLFHWTDGNIHTTALTPRDYQTELLATAREENLIVCIAHNSAKEFLAVKLIQSMRTNRWSSHPEAPGKAIYLTRMDRSLLSSMVSNLTDLQVANVDDVEDSEGSHEPDGASNTPVTDVASADVLFFGSETTLLQYIEQGTVRVQDISLLIVDECHKNYGRQELWEICARLTHQAPSSDRPAQRTRILGLAGPLHGAGCTPERLCWELHYLERCLRARIETASDITSVLRFSTKPTELILECIPPKPSNLTQLLRMLIQRQIAFLKQHRYEPLAVYGLDGNDSASDKPDTDGEPEKDEENDDLRRELKSIPDPTVGPLSYLKQYLELLDEFGPWGADRGALELLTTIDQEKVKVPYDRHFLLFCMVYTTLLQARATVASVFAQHDTELERIKRYSTPKVRRLLEVLAWFGEQRNRPKDRNQPATLHHHQQVHNQQRILYCFCRNVECKELEKSYHTFGAQIADVDERIKQLDGLLASVRKRTERLNLKHKVVDNAAAEGGGTLGVQSPRHGHESRANNFRRKRFAGGGHSHHRSNDTTDALCGLIFCNNRAMARILYVLLYEVSRSQREFEFISPQYTVDKVATNPQNCLKQTTIEHRKQEEVLKRFRMHECNLLIGTSVLEEGIELPKCNLVIRWNSPANYRSYAQCKGRAKAPGAYHVLFVTPENAASRNEQQEDMASIEDVSLDGMIPEGPKDDNTRETIDDQDRRMIETATDAMIEQVAIYREVEKLLLAKCRNGEPPDWELKHADCFNHCLEVYRPSSGGAVALQSNGTCASLWLGNAIQTLNKYCAKLPSDTFTKLTPIWRCATTVRKGRKLYQYTIRLPINSPWKEDILGLPMPTETLARRLAAYMTCRMLHAAGELDNSFQPFGKEAFRAFEADWENFELEESDAKILSENSDPRPGTTKRRQYYYKRIASVFNECRPDAETVAYLYHIRMELICPIPEEQNTRGRKIYAPEESAQGFGILTTKLIPKISSFPIFTRSGEVKVSLDLCPQRVKLSAHQLEMVNCFVKYTFTKVLRLQKSLMLYDANATENCFFIVPTVKQAVAGAAGKDEPPLQSDDVMVDWEFVEKIATNVHRSGPTFIPDEARKGYTFDVGKFRDAVVMPWYRNRDQPQYFYVAEICNHLSPKSTFPGSNYATFEEYYHRKYKIHIQNQRQPLLDVDHTSARLNFLTPRYVNRKGVALPTSSEETKRAKRENLEQKQILVPELCTIHPFPASLWRAAVCLPCVLYRINALLLADEIRRQVARDLRLGWENVDELQEGQFQWPMLSFGWNLADVLRKTKEQKIAQAQEAIDASAPEVEDEVELDKEAPNVRDAAEVDEEDGLKMENGVIAEVEKSQVDGEDDTGDKKTDSDGTLLEIGTWSNEMAVGVGTDNDMGEEGRRGASSPSFLRYDSDCSSNSSANFYSSDEYDEEDDYYLYDGSEKPKNAIEPSQEAVSGTDNANDSGDKPGSRNRTITQSQDTVVNLGGRLKIEFKSETDAEAIDSECDLQRQRTQQSIIERSRQNDMLYQSSKNAVDGFCYSPSDRQCAEEREQAEQRFERQKNHTKDTIRQQGSLVRWNEPLSVSHWRSKLDESEAATLGALMDDLGGQPFTELVPYVEPEQLFELLVRNGSSGERWLRLHDLYCLNRPFFPERYTIFRGGSQFDNFLDESCQEGEGASSPPKVIELTICDPFPAIAAGQMACNYKPKQHSMRSDEANKNGHDSAGVLSTTDEAGGVDYFSFDYQPDLSQHPGPSPAIILQALTMSNANDGINLERLETIGDSFLKYAITTYLYCRYDNVHEGKLSHLRSKQVSNLNLYRLGRRKRLGDCMIAAKFEPHDNWLPPCYYVPKELEQTLIDAKIPACHWNLADLPDIKRLSCAEICQLVKERARAKRREDVDRFDLQHADSNGGEDGNEDDDDDDDDDEDDTTTGEGNEHEADNGSCYIPYNLVTQHSIPDKSVADCVEALIGAYLIECGPRGALLFMAWLGIRVLPIREPPVKLNSNNETALTPYKATGQNDGPLSTGVTIAEYGHWVAPPSPMVRANITFGGIETGAAATSRELARLLQGFEEFEQALGYRFRDRSYLLQAMTHASYSPNRLTDCYQRLEFLGDAILDYLITRHLYEDRRQHSPGALTDLRSALVNNTIFASLAVRHGFHKYFLHLSPGLQEVIDRFVRIQQENGHRITEEEYYLPDEDDELGEYGAMGEDGPGEGRGVGEAEDVEVPKALGDVFESIAGAIFLDSDMSLDTVWKVYRKMMGPEIEKFSSSVPKSPIRELLEMEPETAKFGKPEKLTDGRRVRVTVEVFGKGTFRGIGRNYRIAKCTAAKCALRQLKKLGYANHHKRR

Target ORF information:

RefSeq Version	XM_003436208.1
Organism	Anopheles gambiae str. PEST
Definition	Anopheles gambiae str. PEST AGAP002836-RB (AgaP_AGAP002836), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003436208.1

ORF Insert Sequence:

ATGTCGTTGT TCCACTGGAC GGATGGCAAC ATCCACACCA CAGCCCTCAC GCCCCGGGAC 
TATCAAACCG AGCTGCTGGC TACGGCTCGG GAAGAAAATC TTATCGTCTG TATAGCGCAC 
AATTCCGCCA AAGAGTTCCT GGCCGTAAAG TTGATCCAGT CGATGCGCAC CAATCGCTGG 
AGCAGTCACC CTGAAGCACC GGGAAAAGCC ATCTACCTCA CGCGAATGGA TCGAAGCTTG 
CTGTCGTCGA TGGTTTCGAA TTTGACCGAC CTACAGGTCG CCAACGTCGA TGATGTGGAG 
GACAGTGAAG GGTCACACGA ACCGGACGGT GCTTCCAACA CGCCGGTGAC GGATGTTGCT 
TCGGCCGATG TGCTGTTCTT CGGCAGTGAA ACAACATTGC TGCAGTACAT CGAGCAAGGT 
ACGGTACGGG TGCAAGACAT AAGCCTGTTG ATTGTGGACG AATGCCACAA AAACTACGGT 
CGACAGGAAC TGTGGGAGAT CTGTGCTCGA CTGACGCATC AGGCACCGTC GTCCGATAGG 
CCCGCTCAAC GGACGAGAAT CTTGGGATTA GCTGGTCCAT TGCATGGTGC GGGCTGTACA 
CCGGAACGGT TGTGCTGGGA GCTGCATTAC CTTGAACGAT GCTTGCGTGC ACGTATCGAA 
ACTGCGAGCG ACATCACAAG CGTTCTCAGA TTCAGCACAA AACCTACGGA ACTAATCCTG 
GAATGCATCC CACCCAAGCC ATCCAATTTG ACGCAGCTTC TTAGGATGTT AATACAGCGT 
CAGATCGCTT TCCTGAAGCA GCATCGCTAT GAACCGCTAG CCGTGTACGG ACTGGACGGT 
AACGATAGCG CTAGTGATAA GCCGGACACA GACGGGGAAC CGGAGAAGGA TGAAGAAAAC 
GATGACCTGC GCCGTGAGCT AAAATCCATC CCCGATCCCA CGGTCGGTCC GTTGAGCTAC 
CTAAAGCAGT ATCTCGAGCT GCTGGACGAG TTCGGACCCT GGGGCGCTGA TCGCGGTGCA 
CTCGAACTTC TAACGACCAT CGACCAGGAG AAGGTGAAAG TGCCTTACGA CCGGCACTTT 
CTTCTGTTCT GCATGGTGTA CACCACGCTG CTGCAGGCAA GAGCAACGGT GGCGTCCGTC 
TTCGCACAGC ACGACACCGA ACTGGAGCGC ATCAAGCGCT ATTCGACGCC CAAGGTTCGC 
CGCCTGCTCG AGGTGCTGGC ATGGTTCGGT GAACAACGCA ACCGGCCGAA AGATCGCAAC 
CAACCGGCCA CACTGCACCA TCATCAGCAG GTGCACAATC AGCAGCGCAT ATTGTACTGC 
TTCTGCCGCA ACGTCGAGTG TAAAGAGCTG GAGAAAAGCT ACCACACTTT CGGCGCCCAA 
ATAGCGGACG TTGATGAGCG GATCAAGCAG CTGGACGGTT TGCTAGCATC CGTGCGCAAG 
CGAACCGAAC GGTTGAACCT AAAGCACAAA GTCGTCGACA ATGCGGCCGC CGAGGGTGGT 
GGTACGCTAG GGGTGCAGTC ACCCCGGCAC GGTCACGAGA GCCGAGCAAA CAATTTCCGC 
CGCAAGCGAT TCGCGGGCGG GGGACATTCG CACCACCGAT CGAACGACAC GACCGATGCG 
CTGTGCGGGT TAATTTTCTG CAACAACCGG GCGATGGCCC GCATTCTTTA CGTGCTGCTG 
TACGAGGTGT CTCGTTCGCA GCGCGAATTT GAGTTCATCA GCCCACAGTA CACGGTGGAC 
AAGGTGGCCA CCAATCCGCA AAACTGCCTC AAGCAGACCA CCATCGAGCA TCGCAAGCAG 
GAGGAGGTGC TGAAACGCTT CCGAATGCAC GAATGCAACC TGTTGATTGG GACGTCCGTG 
TTGGAGGAAG GCATCGAGCT GCCAAAGTGT AACCTAGTTA TTCGCTGGAA CAGTCCCGCC 
AACTATCGGT CCTACGCTCA GTGCAAGGGT CGAGCGAAAG CGCCGGGCGC GTACCATGTG 
TTGTTTGTCA CGCCCGAAAA TGCTGCCTCG CGCAACGAAC AACAGGAGGA TATGGCATCG 
ATCGAGGATG TTTCACTTGA TGGCATGATT CCGGAAGGGC CGAAAGACGA CAATACGCGC 
GAAACGATCG ATGATCAGGA CCGAAGAATG ATCGAAACAG CAACAGATGC GATGATCGAG 
CAGGTTGCCA TCTACCGTGA AGTTGAAAAG CTTTTACTTG CCAAGTGCCG CAATGGAGAG 
CCGCCCGATT GGGAGCTGAA GCATGCCGAC TGCTTCAACC ACTGCCTAGA AGTGTATCGT 
CCGAGTAGTG GTGGTGCCGT TGCACTTCAA TCCAACGGAA CGTGCGCCTC ACTGTGGCTG 
GGAAATGCCA TCCAAACACT CAACAAGTAC TGTGCCAAGC TGCCGAGCGA TACGTTCACT 
AAGCTTACTC CGATTTGGCG CTGTGCCACT ACCGTCCGGA AGGGGCGCAA GCTGTACCAA 
TACACCATTC GCCTGCCCAT AAACTCGCCC TGGAAAGAAG ACATATTGGG ATTGCCGATG 
CCTACGGAAA CGCTAGCACG CAGGCTGGCT GCCTATATGA CCTGCCGGAT GCTGCATGCC 
GCTGGGGAGC TTGACAATTC CTTTCAACCG TTCGGCAAGG AAGCCTTTCG TGCGTTCGAA 
GCGGACTGGG AAAACTTTGA GCTGGAGGAA TCGGACGCAA AAATACTGTC CGAAAACAGC 
GACCCCAGAC CGGGCACAAC AAAACGGCGG CAATATTACT ACAAAAGGAT TGCTTCCGTG 
TTCAACGAGT GTCGACCGGA CGCTGAAACC GTCGCCTATC TCTACCACAT ACGCATGGAG 
CTGATCTGCC CCATCCCCGA GGAGCAGAAT ACGCGCGGGC GCAAAATATA CGCACCGGAA 
GAATCCGCAC AAGGGTTTGG CATTCTGACC ACGAAGCTGA TACCAAAGAT AAGCTCCTTT 
CCCATATTCA CCCGGTCCGG CGAGGTGAAA GTGTCGCTCG ATCTGTGCCC GCAGCGCGTT 
AAGCTGTCCG CCCACCAGCT GGAGATGGTA AACTGTTTCG TGAAGTACAC GTTCACCAAA 
GTGTTGCGCT TGCAGAAGAG CCTGATGCTG TACGATGCGA ACGCAACGGA GAACTGTTTC 
TTCATCGTTC CGACGGTCAA ACAAGCTGTT GCGGGTGCGG CTGGTAAAGA CGAACCACCG 
CTCCAATCAG ACGACGTGAT GGTGGACTGG GAGTTTGTGG AAAAGATTGC CACGAACGTG 
CATCGCAGCG GACCAACATT CATACCGGAT GAGGCACGCA AGGGCTACAC GTTCGATGTG 
GGCAAATTTC GGGACGCCGT TGTAATGCCG TGGTATCGGA ACCGCGACCA GCCGCAGTAC 
TTTTACGTGG CGGAAATATG CAACCATCTG TCGCCGAAGA GCACCTTTCC CGGGTCGAAC 
TACGCGACGT TCGAGGAGTA TTACCATCGC AAGTACAAAA TCCACATTCA GAACCAACGC 
CAGCCGCTGC TGGACGTTGA CCATACCAGT GCGCGGCTAA ACTTCCTGAC GCCCCGTTAC 
GTTAACCGGA AGGGTGTAGC GTTGCCGACC AGCTCGGAGG AGACGAAGCG GGCCAAGCGT 
GAAAACTTGG AGCAGAAACA GATCCTCGTG CCGGAGCTGT GCACGATACA TCCGTTCCCT 
GCCTCGCTGT GGCGTGCGGC CGTCTGCCTG CCGTGCGTAC TGTACCGCAT CAACGCGCTG 
CTGCTGGCAG ATGAGATACG GCGGCAGGTG GCACGCGACC TGCGGCTGGG ATGGGAAAAT 
GTGGACGAGC TGCAGGAGGG CCAGTTCCAG TGGCCAATGC TAAGCTTTGG CTGGAATCTG 
GCCGATGTGC TGCGCAAAAC GAAGGAGCAG AAAATTGCGC AAGCTCAGGA AGCGATCGAT 
GCCAGTGCGC CGGAAGTGGA GGATGAAGTG GAGCTAGACA AGGAAGCGCC GAACGTGCGT 
GATGCTGCGG AGGTTGATGA GGAAGATGGG CTAAAAATGG AAAACGGTGT AATTGCTGAG 
GTTGAAAAGA GTCAGGTGGA TGGAGAGGAT GATACTGGCG ATAAAAAGAC TGATAGCGAT 
GGAACGCTGC TTGAAATTGG TACATGGTCG AACGAAATGG CGGTAGGCGT TGGAACCGAT 
AACGATATGG GTGAAGAAGG TAGGAGAGGC GCATCGTCAC CCTCGTTCCT GCGGTACGAT 
TCCGACTGTT CCAGCAACAG CAGTGCCAAT TTCTACTCTT CGGACGAGTA CGACGAGGAG 
GACGATTACT ACCTGTACGA TGGTTCGGAA AAGCCGAAAA ATGCGATAGA ACCGAGCCAG 
GAGGCAGTAT CGGGCACGGA CAATGCGAAC GACTCGGGCG ATAAGCCGGG CAGTAGGAAC 
CGGACCATCA CGCAGTCACA GGACACGGTG GTAAATCTGG GTGGGCGGTT GAAGATCGAG 
TTCAAGTCGG AAACCGATGC CGAAGCGATC GATTCCGAGT GTGATCTGCA GCGGCAGCGA 
ACGCAGCAGA GCATCATTGA GCGGTCACGC CAGAACGATA TGCTCTATCA GAGCTCGAAA 
AACGCGGTCG ATGGGTTCTG CTACTCGCCG AGCGATCGGC AGTGCGCCGA GGAGCGGGAG 
CAAGCGGAGC AACGGTTTGA GCGGCAGAAA AATCACACCA AAGACACCAT CCGCCAGCAG 
GGCAGCTTGG TGCGATGGAA CGAACCGCTG TCCGTAAGCC ACTGGCGATC GAAGCTGGAC 
GAGTCGGAAG CGGCCACCCT TGGCGCACTG ATGGACGATT TGGGCGGGCA ACCGTTCACG 
GAGCTCGTAC CGTACGTCGA GCCGGAGCAG CTGTTCGAGC TGCTTGTGCG AAACGGTAGC 
AGCGGGGAAC GATGGCTCCG ATTGCACGAT CTCTACTGCT TGAATCGACC GTTCTTCCCG 
GAACGGTACA CCATCTTCCG GGGCGGTTCA CAGTTTGATA ACTTTCTGGA CGAAAGCTGC 
CAGGAAGGTG AGGGTGCTAG TTCGCCACCG AAAGTGATCG AGCTGACTAT TTGCGATCCT 
TTTCCGGCGA TAGCCGCCGG GCAGATGGCT TGCAACTACA AACCGAAACA ACATTCCATG 
CGGAGCGACG AAGCGAACAA GAACGGCCAC GACAGTGCGG GTGTGCTCAG TACGACAGAT 
GAGGCCGGGG GTGTTGATTA CTTCAGTTTC GATTATCAGC CAGATTTGAG CCAACATCCC 
GGTCCCAGTC CGGCTATTAT TCTACAAGCG CTAACCATGT CGAACGCCAA CGATGGCATC 
AACCTGGAGC GATTGGAAAC GATTGGCGAT TCCTTTCTGA AGTACGCCAT CACCACCTAC 
CTTTACTGCC GGTACGACAA CGTGCACGAG GGCAAGCTAA GCCACCTGCG GTCGAAGCAG 
GTATCCAATC TGAACCTTTA CCGGTTGGGG CGCAGGAAGC GGTTGGGCGA CTGCATGATT 
GCGGCCAAAT TTGAACCACA CGATAACTGG CTGCCACCCT GCTACTACGT GCCGAAGGAG 
CTAGAACAAA CGTTGATTGA TGCAAAGATC CCTGCTTGCC ATTGGAATCT GGCCGATCTT 
CCAGACATCA AGCGTCTGTC GTGCGCTGAA ATCTGTCAAC TAGTCAAGGA ACGTGCACGG 
GCCAAACGCA GAGAGGATGT CGACCGGTTC GACCTACAAC ATGCAGACAG CAACGGTGGT 
GAGGACGGAA ATGAAGACGA CGACGATGAC GACGACGACG ATGAAGACGA TACAACCACT 
GGCGAAGGGA ACGAGCACGA AGCGGACAAT GGGTCCTGCT ACATCCCGTA CAATTTAGTA 
ACGCAGCACA GCATCCCCGA CAAATCGGTG GCTGACTGTG TGGAAGCCCT GATCGGTGCC 
TACCTCATCG AATGTGGCCC CCGCGGAGCG CTCCTCTTTA TGGCATGGCT TGGCATACGG 
GTGCTCCCCA TCCGTGAGCC GCCGGTGAAG CTTAATTCAA ACAACGAGAC GGCGCTTACT 
CCTTACAAAG CTACTGGACA GAACGATGGA CCCCTGTCCA CTGGCGTAAC GATTGCTGAG 
TATGGTCACT GGGTTGCACC GCCCTCGCCC ATGGTGCGGG CCAACATTAC GTTCGGTGGC 
ATCGAAACCG GTGCCGCGGC AACGTCCCGC GAGCTGGCCC GGCTGCTGCA AGGGTTCGAG 
GAGTTCGAGC AGGCGCTTGG CTACCGCTTC CGGGACCGTT CCTACCTGCT GCAAGCCATG 
ACGCACGCTT CGTACAGCCC GAACCGGCTG ACGGATTGCT ACCAGCGGCT GGAGTTTCTG 
GGCGATGCCA TCCTCGACTA CCTCATCACG CGCCATCTGT ACGAGGACCG CCGGCAGCAT 
TCACCGGGCG CGCTCACAGA CTTGCGGTCG GCGCTGGTGA ACAATACCAT CTTTGCTTCG 
CTCGCCGTAC GCCACGGATT TCATAAGTAC TTCCTGCACC TGTCGCCCGG GCTGCAGGAG 
GTGATCGATC GATTCGTTCG CATCCAGCAA GAGAACGGGC ATCGCATCAC GGAGGAGGAA 
TACTACCTGC CGGACGAAGA TGACGAGCTG GGCGAGTACG GTGCGATGGG GGAGGACGGT 
CCCGGCGAGG GCCGCGGTGT GGGGGAGGCG GAAGATGTGG AAGTGCCGAA AGCGTTGGGC 
GACGTGTTCG AATCGATTGC CGGTGCCATT TTCCTCGATT CCGACATGTC GCTCGATACG 
GTGTGGAAGG TGTATCGGAA AATGATGGGA CCGGAAATAG AGAAATTCAG CAGCTCGGTC 
CCAAAATCCC CGATACGCGA ACTGCTCGAA ATGGAACCGG AGACGGCCAA ATTTGGCAAA 
CCGGAGAAGC TGACGGACGG ACGGCGCGTG CGGGTAACGG TGGAAGTGTT CGGCAAAGGT 
ACATTCCGTG GCATTGGAAG GAACTATCGC ATCGCCAAGT GTACGGCGGC AAAGTGTGCC 
CTTCGCCAGC TCAAGAAGCT CGGCTATGCC AACCATCACA AGCGCCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AgaP_AGAP002836 ( XM_312076.3 ) cDNA ORF clone, Anopheles gambiae str. PEST -> XP_312076.2 Anopheles gambiae str. PEST AGAP002836-RA (AgaP_AGAP002836), partial mRNA.

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CloneID	OAh02781
Clone ID Related Accession (Same CDS sequence)	XM_312076.3
Accession Version	XM_312076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-25
Organism	Anopheles gambiae str. PEST
Product	AGAP002836-PA
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_078266). On Dec 6, 2006 this sequence version replaced XM_312076.2. COMPLETENESS: incomplete on both ends.

ATGTCGTTGT TCCACTGGAC GGATGGCAAC ATCCACACCA CAGCCCTCAC GCCCCGGGAC 
TATCAAACCG AGCTGCTGGC TACGGCTCGG GAAGAAAATC TTATCGTCTG TATAGCGCAC 
AATTCCGCCA AAGAGTTCCT GGCCGTAAAG TTGATCCAGT CGATGCGCAC CAATCGCTGG 
AGCAGTCACC CTGAAGCACC GGGAAAAGCC ATCTACCTCA CGCGAATGGA TCGAAGCTTG 
CTGTCGTCGA TGGTTTCGAA TTTGACCGAC CTACAGGTCG CCAACGTCGA TGATGTGGAG 
GACAGTGAAG GGTCACACGA ACCGGACGGT GCTTCCAACA CGCCGGTGAC GGATGTTGCT 
TCGGCCGATG TGCTGTTCTT CGGCAGTGAA ACAACATTGC TGCAGTACAT CGAGCAAGGT 
ACGGTACGGG TGCAAGACAT AAGCCTGTTG ATTGTGGACG AATGCCACAA AAACTACGGT 
CGACAGGAAC TGTGGGAGAT CTGTGCTCGA CTGACGCATC AGGCACCGTC GTCCGATAGG 
CCCGCTCAAC GGACGAGAAT CTTGGGATTA GCTGGTCCAT TGCATGGTGC GGGCTGTACA 
CCGGAACGGT TGTGCTGGGA GCTGCATTAC CTTGAACGAT GCTTGCGTGC ACGTATCGAA 
ACTGCGAGCG ACATCACAAG CGTTCTCAGA TTCAGCACAA AACCTACGGA ACTAATCCTG 
GAATGCATCC CACCCAAGCC ATCCAATTTG ACGCAGCTTC TTAGGATGTT AATACAGCGT 
CAGATCGCTT TCCTGAAGCA GCATCGCTAT GAACCGCTAG CCGTGTACGG ACTGGACGGT 
AACGATAGCG CTAGTGATAA GCCGGACACA GACGGGGAAC CGGAGAAGGA TGAAGAAAAC 
GATGACCTGC GCCGTGAGCT AAAATCCATC CCCGATCCCA CGGTCGGTCC GTTGAGCTAC 
CTAAAGCAGT ATCTCGAGCT GCTGGACGAG TTCGGACCCT GGGGCGCTGA TCGCGGTGCA 
CTCGAACTTC TAACGACCAT CGACCAGGAG AAGGTGAAAG TGCCTTACGA CCGGCACTTT 
CTTCTGTTCT GCATGGTGTA CACCACGCTG CTGCAGGCAA GAGCAACGGT GGCGTCCGTC 
TTCGCACAGC ACGACACCGA ACTGGAGCGC ATCAAGCGCT ATTCGACGCC CAAGGTTCGC 
CGCCTGCTCG AGGTGCTGGC ATGGTTCGGT GAACAACGCA ACCGGCCGAA AGATCGCAAC 
CAACCGGCCA CACTGCACCA TCATCAGCAG GTGCACAATC AGCAGCGCAT ATTGTACTGC 
TTCTGCCGCA ACGTCGAGTG TAAAGAGCTG GAGAAAAGCT ACCACACTTT CGGCGCCCAA 
ATAGCGGACG TTGATGAGCG GATCAAGCAG CTGGACGGTT TGCTAGCATC CGTGCGCAAG 
CGAACCGAAC GGTTGAACCT AAAGCACAAA GTCGTCGACA ATGCGGCCGC CGAGGGTGGT 
GGTACGCTAG GGGTGCAGTC ACCCCGGCAC GGTCACGAGA GCCGAGCAAA CAATTTCCGC 
CGCAAGCGAT TCGCGGGCGG GGGACATTCG CACCACCGAT CGAACGACAC GACCGATGCG 
CTGTGCGGGT TAATTTTCTG CAACAACCGG GCGATGGCCC GCATTCTTTA CGTGCTGCTG 
TACGAGGTGT CTCGTTCGCA GCGCGAATTT GAGTTCATCA GCCCACAGTA CACGGTGGAC 
AAGGTGGCCA CCAATCCGCA AAACTGCCTC AAGCAGACCA CCATCGAGCA TCGCAAGCAG 
GAGGAGGTGC TGAAACGCTT CCGAATGCAC GAATGCAACC TGTTGATTGG GACGTCCGTG 
TTGGAGGAAG GCATCGAGCT GCCAAAGTGT AACCTAGTTA TTCGCTGGAA CAGTCCCGCC 
AACTATCGGT CCTACGCTCA GTGCAAGGGT CGAGCGAAAG CGCCGGGCGC GTACCATGTG 
TTGTTTGTCA CGCCCGAAAA TGCTGCCTCG CGCAACGAAC AACAGGAGGA TATGGCATCG 
ATCGAGGATG TTTCACTTGA TGGCATGATT CCGGAAGGGC CGAAAGACGA CAATACGCGC 
GAAACGATCG ATGATCAGGA CCGAAGAATG ATCGAAACAG CAACAGATGC GATGATCGAG 
CAGGTTGCCA TCTACCGTGA AGTTGAAAAG CTTTTACTTG CCAAGTGCCG CAATGGAGAG 
CCGCCCGATT GGGAGCTGAA GCATGCCGAC TGCTTCAACC ACTGCCTAGA AGTGTATCGT 
CCGAGTAGTG GTGGTGCCGT TGCACTTCAA TCCAACGGAA CGTGCGCCTC ACTGTGGCTG 
GGAAATGCCA TCCAAACACT CAACAAGTAC TGTGCCAAGC TGCCGAGCGA TACGTTCACT 
AAGCTTACTC CGATTTGGCG CTGTGCCACT ACCGTCCGGA AGGGGCGCAA GCTGTACCAA 
TACACCATTC GCCTGCCCAT AAACTCGCCC TGGAAAGAAG ACATATTGAT TGCTTCCGTG 
TTCAACGAGT GTCGACCGGA CGCTGAAACC GTCGCCTATC TCTACCACAT ACGCATGGAG 
CTGATCTGCC CCATCCCCGA GGAGCAGAAT ACGCGCGGGC GCAAAATATA CGCACCGGAA 
GAATCCGCAC AAGGGTTTGG CATTCTGACC ACGAAGCTGA TACCAAAGAT AAGCTCCTTT 
CCCATATTCA CCCGGTCCGG CGAGGTGAAA GTGTCGCTCG ATCTGTGCCC GCAGCGCGTT 
AAGCTGTCCG CCCACCAGCT GGAGATGGTA AACTGTTTCG TGAAGTACAC GTTCACCAAA 
GTGTTGCGCT TGCAGAAGAG CCTGATGCTG TACGATGCGA ACGCAACGGA GAACTGTTTC 
TTCATCGTTC CGACGGTCAA ACAAGCTGTT GCGGGTGCGG CTGGTAAAGA CGAACCACCG 
CTCCAATCAG ACGACGTGAT GGTGGACTGG GAGTTTGTGG AAAAGATTGC CACGAACGTG 
CATCGCAGCG GACCAACATT CATACCGGAT GAGGCACGCA AGGGCTACAC GTTCGATGTG 
GGCAAATTTC GGGACGCCGT TGTAATGCCG TGGTATCGGA ACCGCGACCA GCCGCAGTAC 
TTTTACGTGG CGGAAATATG CAACCATCTG TCGCCGAAGA GCACCTTTCC CGGGTCGAAC 
TACGCGACGT TCGAGGAGTA TTACCATCGC AAGTACAAAA TCCACATTCA GAACCAACGC 
CAGCCGCTGC TGGACGTTGA CCATACCAGT GCGCGGCTAA ACTTCCTGAC GCCCCGTTAC 
GTTAACCGGA AGGGTGTAGC GTTGCCGACC AGCTCGGAGG AGACGAAGCG GGCCAAGCGT 
GAAAACTTGG AGCAGAAACA GATCCTCGTG CCGGAGCTGT GCACGATACA TCCGTTCCCT 
GCCTCGCTGT GGCGTGCGGC CGTCTGCCTG CCGTGCGTAC TGTACCGCAT CAACGCGCTG 
CTGCTGGCAG ATGAGATACG GCGGCAGGTG GCACGCGACC TGCGGCTGGG ATGGGAAAAT 
GTGGACGAGC TGCAGGAGGG CCAGTTCCAG TGGCCAATGC TAAGCTTTGG CTGGAATCTG 
GCCGATGTGC TGCGCAAAAC GAAGGAGCAG AAAATTGCGC AAGCTCAGGA AGCGATCGAT 
GCCAGTGCGC CGGAAGTGGA GGATGAAGTG GAGCTAGACA AGGAAGCGCC GAACGTGCGT 
GATGCTGCGG AGGTTGATGA GGAAGATGGG CTAAAAATGG AAAACGGTGT AATTGCTGAG 
GTTGAAAAGA GTCAGGTGGA TGGAGAGGAT GATACTGGCG ATAAAAAGAC TGATAGCGAT 
GGAACGCTGC TTGAAATTGG TACATGGTCG AACGAAATGG CGGTAGGCGT TGGAACCGAT 
AACGATATGG GTGAAGAAGG TAGGAGAGGC GCATCGTCAC CCTCGTTCCT GCGGTACGAT 
TCCGACTGTT CCAGCAACAG CAGTGCCAAT TTCTACTCTT CGGACGAGTA CGACGAGGAG 
GACGATTACT ACCTGTACGA TGGTTCGGAA AAGCCGAAAA ATGCGATAGA ACCGAGCCAG 
GAGGCAGTAT CGGGCACGGA CAATGCGAAC GACTCGGGCG ATAAGCCGGG CAGTAGGAAC 
CGGACCATCA CGCAGTCACA GGACACGGTG GTAAATCTGG GTGGGCGGTT GAAGATCGAG 
TTCAAGTCGG AAACCGATGC CGAAGCGATC GATTCCGAGT GTGATCTGCA GCGGCAGCGA 
ACGCAGCAGA GCATCATTGA GCGGTCACGC CAGAACGATA TGCTCTATCA GAGCTCGAAA 
AACGCGGTCG ATGGGTTCTG CTACTCGCCG AGCGATCGGC AGTGCGCCGA GGAGCGGGAG 
CAAGCGGAGC AACGGTTTGA GCGGCAGAAA AATCACACCA AAGACACCAT CCGCCAGCAG 
GGCAGCTTGG TGCGATGGAA CGAACCGCTG TCCGTAAGCC ACTGGCGATC GAAGCTGGAC 
GAGTCGGAAG CGGCCACCCT TGGCGCACTG ATGGACGATT TGGGCGGGCA ACCGTTCACG 
GAGCTCGTAC CGTACGTCGA GCCGGAGCAG CTGTTCGAGC TGCTTGTGCG AAACGGTAGC 
AGCGGGGAAC GATGGCTCCG ATTGCACGAT CTCTACTGCT TGAATCGACC GTTCTTCCCG 
GAACGGTACA CCATCTTCCG GGGCGGTTCA CAGTTTGATA ACTTTCTGGA CGAAAGCTGC 
CAGGAAGGTG AGGGTGCTAG TTCGCCACCG AAAGTGATCG AGCTGACTAT TTGCGATCCT 
TTTCCGGCGA TAGCCGCCGG GCAGATGGCT TGCAACTACA AACCGAAACA ACATTCCATG 
CGGAGCGACG AAGCGAACAA GAACGGCCAC GACAGTGCGG GTGTGCTCAG TACGACAGAT 
GAGGCCGGGG GTGTTGATTA CTTCAGTTTC GATTATCAGC CAGATTTGAG CCAACATCCC 
GGTCCCAGTC CGGCTATTAT TCTACAAGCG CTAACCATGT CGAACGCCAA CGATGGCATC 
AACCTGGAGC GATTGGAAAC GATTGGCGAT TCCTTTCTGA AGTACGCCAT CACCACCTAC 
CTTTACTGCC GGTACGACAA CGTGCACGAG GGCAAGCTAA GCCACCTGCG GTCGAAGCAG 
GTATCCAATC TGAACCTTTA CCGGTTGGGG CGCAGGAAGC GGTTGGGCGA CTGCATGATT 
GCGGCCAAAT TTGAACCACA CGATAACTGG CTGCCACCCT GCTACTACGT GCCGAAGGAG 
CTAGAACAAA CGTTGATTGA TGCAAAGATC CCTGCTTGCC ATTGGAATCT GGCCGATCTT 
CCAGACATCA AGCGTCTGTC GTGCGCTGAA ATCTGTCAAC TAGTCAAGGA ACGTGCACGG 
GCCAAACGCA GAGAGGATGT CGACCGGTTC GACCTACAAC ATGCAGACAG CAACGGTGGT 
GAGGACGGAA ATGAAGACGA CGACGATGAC GACGACGACG ATGAAGACGA TACAACCACT 
GGCGAAGGGA ACGAGCACGA AGCGGACAAT GGGTCCTGCT ACATCCCGTA CAATTTAGTA 
ACGCAGCACA GCATCCCCGA CAAATCGGTG GCTGACTGTG TGGAAGCCCT GATCGGTGCC 
TACCTCATCG AATGTGGCCC CCGCGGAGCG CTCCTCTTTA TGGCATGGCT TGGCATACGG 
GTGCTCCCCA TCCGTGAGCC GCCGGTGAAG CTTAATTCAA ACAACGAGAC GGCGCTTACT 
CCTTACAAAG CTACTGGACA GAACGATGGA CCCCTGTCCA CTGGCGTAAC GATTGCTGAG 
TATGGTCACT GGGTTGCACC GCCCTCGCCC ATGGTGCGGG CCAACATTAC GTTCGGTGGC 
ATCGAAACCG GTGCCGCGGC AACGTCCCGC GAGCTGGCCC GGCTGCTGCA AGGGTTCGAG 
GAGTTCGAGC AGGCGCTTGG CTACCGCTTC CGGGACCGTT CCTACCTGCT GCAAGCCATG 
ACGCACGCTT CGTACAGCCC GAACCGGCTG ACGGATTGCT ACCAGCGGCT GGAGTTTCTG 
GGCGATGCCA TCCTCGACTA CCTCATCACG CGCCATCTGT ACGAGGACCG CCGGCAGCAT 
TCACCGGGCG CGCTCACAGA CTTGCGGTCG GCGCTGGTGA ACAATACCAT CTTTGCTTCG 
CTCGCCGTAC GCCACGGATT TCATAAGTAC TTCCTGCACC TGTCGCCCGG GCTGCAGGAG 
GTGATCGATC GATTCGTTCG CATCCAGCAA GAGAACGGGC ATCGCATCAC GGAGGAGGAA 
TACTACCTGC CGGACGAAGA TGACGAGCTG GGCGAGTACG GTGCGATGGG GGAGGACGGT 
CCCGGCGAGG GCCGCGGTGT GGGGGAGGCG GAAGATGTGG AAGTGCCGAA AGCGTTGGGC 
GACGTGTTCG AATCGATTGC CGGTGCCATT TTCCTCGATT CCGACATGTC GCTCGATACG 
GTGTGGAAGG TGTATCGGAA AATGATGGGA CCGGAAATAG AGAAATTCAG CAGCTCGGTC 
CCAAAATCCC CGATACGCGA ACTGCTCGAA ATGGAACCGG AGACGGCCAA ATTTGGCAAA 
CCGGAGAAGC TGACGGACGG ACGGCGCGTG CGGGTAACGG TGGAAGTGTT CGGCAAAGGT 
ACATTCCGTG GCATTGGAAG GAACTATCGC ATCGCCAAGT GTACGGCGGC AAAGTGTGCC 
CTTCGCCAGC TCAAGAAGCT CGGCTATGCC AACCATCACA AGCGCCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_312076.2
CDS	1..6771
Translation	MSLFHWTDGNIHTTALTPRDYQTELLATAREENLIVCIAHNSAKEFLAVKLIQSMRTNRWSSHPEAPGKAIYLTRMDRSLLSSMVSNLTDLQVANVDDVEDSEGSHEPDGASNTPVTDVASADVLFFGSETTLLQYIEQGTVRVQDISLLIVDECHKNYGRQELWEICARLTHQAPSSDRPAQRTRILGLAGPLHGAGCTPERLCWELHYLERCLRARIETASDITSVLRFSTKPTELILECIPPKPSNLTQLLRMLIQRQIAFLKQHRYEPLAVYGLDGNDSASDKPDTDGEPEKDEENDDLRRELKSIPDPTVGPLSYLKQYLELLDEFGPWGADRGALELLTTIDQEKVKVPYDRHFLLFCMVYTTLLQARATVASVFAQHDTELERIKRYSTPKVRRLLEVLAWFGEQRNRPKDRNQPATLHHHQQVHNQQRILYCFCRNVECKELEKSYHTFGAQIADVDERIKQLDGLLASVRKRTERLNLKHKVVDNAAAEGGGTLGVQSPRHGHESRANNFRRKRFAGGGHSHHRSNDTTDALCGLIFCNNRAMARILYVLLYEVSRSQREFEFISPQYTVDKVATNPQNCLKQTTIEHRKQEEVLKRFRMHECNLLIGTSVLEEGIELPKCNLVIRWNSPANYRSYAQCKGRAKAPGAYHVLFVTPENAASRNEQQEDMASIEDVSLDGMIPEGPKDDNTRETIDDQDRRMIETATDAMIEQVAIYREVEKLLLAKCRNGEPPDWELKHADCFNHCLEVYRPSSGGAVALQSNGTCASLWLGNAIQTLNKYCAKLPSDTFTKLTPIWRCATTVRKGRKLYQYTIRLPINSPWKEDILIASVFNECRPDAETVAYLYHIRMELICPIPEEQNTRGRKIYAPEESAQGFGILTTKLIPKISSFPIFTRSGEVKVSLDLCPQRVKLSAHQLEMVNCFVKYTFTKVLRLQKSLMLYDANATENCFFIVPTVKQAVAGAAGKDEPPLQSDDVMVDWEFVEKIATNVHRSGPTFIPDEARKGYTFDVGKFRDAVVMPWYRNRDQPQYFYVAEICNHLSPKSTFPGSNYATFEEYYHRKYKIHIQNQRQPLLDVDHTSARLNFLTPRYVNRKGVALPTSSEETKRAKRENLEQKQILVPELCTIHPFPASLWRAAVCLPCVLYRINALLLADEIRRQVARDLRLGWENVDELQEGQFQWPMLSFGWNLADVLRKTKEQKIAQAQEAIDASAPEVEDEVELDKEAPNVRDAAEVDEEDGLKMENGVIAEVEKSQVDGEDDTGDKKTDSDGTLLEIGTWSNEMAVGVGTDNDMGEEGRRGASSPSFLRYDSDCSSNSSANFYSSDEYDEEDDYYLYDGSEKPKNAIEPSQEAVSGTDNANDSGDKPGSRNRTITQSQDTVVNLGGRLKIEFKSETDAEAIDSECDLQRQRTQQSIIERSRQNDMLYQSSKNAVDGFCYSPSDRQCAEEREQAEQRFERQKNHTKDTIRQQGSLVRWNEPLSVSHWRSKLDESEAATLGALMDDLGGQPFTELVPYVEPEQLFELLVRNGSSGERWLRLHDLYCLNRPFFPERYTIFRGGSQFDNFLDESCQEGEGASSPPKVIELTICDPFPAIAAGQMACNYKPKQHSMRSDEANKNGHDSAGVLSTTDEAGGVDYFSFDYQPDLSQHPGPSPAIILQALTMSNANDGINLERLETIGDSFLKYAITTYLYCRYDNVHEGKLSHLRSKQVSNLNLYRLGRRKRLGDCMIAAKFEPHDNWLPPCYYVPKELEQTLIDAKIPACHWNLADLPDIKRLSCAEICQLVKERARAKRREDVDRFDLQHADSNGGEDGNEDDDDDDDDDEDDTTTGEGNEHEADNGSCYIPYNLVTQHSIPDKSVADCVEALIGAYLIECGPRGALLFMAWLGIRVLPIREPPVKLNSNNETALTPYKATGQNDGPLSTGVTIAEYGHWVAPPSPMVRANITFGGIETGAAATSRELARLLQGFEEFEQALGYRFRDRSYLLQAMTHASYSPNRLTDCYQRLEFLGDAILDYLITRHLYEDRRQHSPGALTDLRSALVNNTIFASLAVRHGFHKYFLHLSPGLQEVIDRFVRIQQENGHRITEEEYYLPDEDDELGEYGAMGEDGPGEGRGVGEAEDVEVPKALGDVFESIAGAIFLDSDMSLDTVWKVYRKMMGPEIEKFSSSVPKSPIRELLEMEPETAKFGKPEKLTDGRRVRVTVEVFGKGTFRGIGRNYRIAKCTAAKCALRQLKKLGYANHHKRR

Target ORF information:

RefSeq Version	XM_312076.3
Organism	Anopheles gambiae str. PEST
Definition	Anopheles gambiae str. PEST AGAP002836-RA (AgaP_AGAP002836), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_312076.3

ORF Insert Sequence:

ATGTCGTTGT TCCACTGGAC GGATGGCAAC ATCCACACCA CAGCCCTCAC GCCCCGGGAC 
TATCAAACCG AGCTGCTGGC TACGGCTCGG GAAGAAAATC TTATCGTCTG TATAGCGCAC 
AATTCCGCCA AAGAGTTCCT GGCCGTAAAG TTGATCCAGT CGATGCGCAC CAATCGCTGG 
AGCAGTCACC CTGAAGCACC GGGAAAAGCC ATCTACCTCA CGCGAATGGA TCGAAGCTTG 
CTGTCGTCGA TGGTTTCGAA TTTGACCGAC CTACAGGTCG CCAACGTCGA TGATGTGGAG 
GACAGTGAAG GGTCACACGA ACCGGACGGT GCTTCCAACA CGCCGGTGAC GGATGTTGCT 
TCGGCCGATG TGCTGTTCTT CGGCAGTGAA ACAACATTGC TGCAGTACAT CGAGCAAGGT 
ACGGTACGGG TGCAAGACAT AAGCCTGTTG ATTGTGGACG AATGCCACAA AAACTACGGT 
CGACAGGAAC TGTGGGAGAT CTGTGCTCGA CTGACGCATC AGGCACCGTC GTCCGATAGG 
CCCGCTCAAC GGACGAGAAT CTTGGGATTA GCTGGTCCAT TGCATGGTGC GGGCTGTACA 
CCGGAACGGT TGTGCTGGGA GCTGCATTAC CTTGAACGAT GCTTGCGTGC ACGTATCGAA 
ACTGCGAGCG ACATCACAAG CGTTCTCAGA TTCAGCACAA AACCTACGGA ACTAATCCTG 
GAATGCATCC CACCCAAGCC ATCCAATTTG ACGCAGCTTC TTAGGATGTT AATACAGCGT 
CAGATCGCTT TCCTGAAGCA GCATCGCTAT GAACCGCTAG CCGTGTACGG ACTGGACGGT 
AACGATAGCG CTAGTGATAA GCCGGACACA GACGGGGAAC CGGAGAAGGA TGAAGAAAAC 
GATGACCTGC GCCGTGAGCT AAAATCCATC CCCGATCCCA CGGTCGGTCC GTTGAGCTAC 
CTAAAGCAGT ATCTCGAGCT GCTGGACGAG TTCGGACCCT GGGGCGCTGA TCGCGGTGCA 
CTCGAACTTC TAACGACCAT CGACCAGGAG AAGGTGAAAG TGCCTTACGA CCGGCACTTT 
CTTCTGTTCT GCATGGTGTA CACCACGCTG CTGCAGGCAA GAGCAACGGT GGCGTCCGTC 
TTCGCACAGC ACGACACCGA ACTGGAGCGC ATCAAGCGCT ATTCGACGCC CAAGGTTCGC 
CGCCTGCTCG AGGTGCTGGC ATGGTTCGGT GAACAACGCA ACCGGCCGAA AGATCGCAAC 
CAACCGGCCA CACTGCACCA TCATCAGCAG GTGCACAATC AGCAGCGCAT ATTGTACTGC 
TTCTGCCGCA ACGTCGAGTG TAAAGAGCTG GAGAAAAGCT ACCACACTTT CGGCGCCCAA 
ATAGCGGACG TTGATGAGCG GATCAAGCAG CTGGACGGTT TGCTAGCATC CGTGCGCAAG 
CGAACCGAAC GGTTGAACCT AAAGCACAAA GTCGTCGACA ATGCGGCCGC CGAGGGTGGT 
GGTACGCTAG GGGTGCAGTC ACCCCGGCAC GGTCACGAGA GCCGAGCAAA CAATTTCCGC 
CGCAAGCGAT TCGCGGGCGG GGGACATTCG CACCACCGAT CGAACGACAC GACCGATGCG 
CTGTGCGGGT TAATTTTCTG CAACAACCGG GCGATGGCCC GCATTCTTTA CGTGCTGCTG 
TACGAGGTGT CTCGTTCGCA GCGCGAATTT GAGTTCATCA GCCCACAGTA CACGGTGGAC 
AAGGTGGCCA CCAATCCGCA AAACTGCCTC AAGCAGACCA CCATCGAGCA TCGCAAGCAG 
GAGGAGGTGC TGAAACGCTT CCGAATGCAC GAATGCAACC TGTTGATTGG GACGTCCGTG 
TTGGAGGAAG GCATCGAGCT GCCAAAGTGT AACCTAGTTA TTCGCTGGAA CAGTCCCGCC 
AACTATCGGT CCTACGCTCA GTGCAAGGGT CGAGCGAAAG CGCCGGGCGC GTACCATGTG 
TTGTTTGTCA CGCCCGAAAA TGCTGCCTCG CGCAACGAAC AACAGGAGGA TATGGCATCG 
ATCGAGGATG TTTCACTTGA TGGCATGATT CCGGAAGGGC CGAAAGACGA CAATACGCGC 
GAAACGATCG ATGATCAGGA CCGAAGAATG ATCGAAACAG CAACAGATGC GATGATCGAG 
CAGGTTGCCA TCTACCGTGA AGTTGAAAAG CTTTTACTTG CCAAGTGCCG CAATGGAGAG 
CCGCCCGATT GGGAGCTGAA GCATGCCGAC TGCTTCAACC ACTGCCTAGA AGTGTATCGT 
CCGAGTAGTG GTGGTGCCGT TGCACTTCAA TCCAACGGAA CGTGCGCCTC ACTGTGGCTG 
GGAAATGCCA TCCAAACACT CAACAAGTAC TGTGCCAAGC TGCCGAGCGA TACGTTCACT 
AAGCTTACTC CGATTTGGCG CTGTGCCACT ACCGTCCGGA AGGGGCGCAA GCTGTACCAA 
TACACCATTC GCCTGCCCAT AAACTCGCCC TGGAAAGAAG ACATATTGAT TGCTTCCGTG 
TTCAACGAGT GTCGACCGGA CGCTGAAACC GTCGCCTATC TCTACCACAT ACGCATGGAG 
CTGATCTGCC CCATCCCCGA GGAGCAGAAT ACGCGCGGGC GCAAAATATA CGCACCGGAA 
GAATCCGCAC AAGGGTTTGG CATTCTGACC ACGAAGCTGA TACCAAAGAT AAGCTCCTTT 
CCCATATTCA CCCGGTCCGG CGAGGTGAAA GTGTCGCTCG ATCTGTGCCC GCAGCGCGTT 
AAGCTGTCCG CCCACCAGCT GGAGATGGTA AACTGTTTCG TGAAGTACAC GTTCACCAAA 
GTGTTGCGCT TGCAGAAGAG CCTGATGCTG TACGATGCGA ACGCAACGGA GAACTGTTTC 
TTCATCGTTC CGACGGTCAA ACAAGCTGTT GCGGGTGCGG CTGGTAAAGA CGAACCACCG 
CTCCAATCAG ACGACGTGAT GGTGGACTGG GAGTTTGTGG AAAAGATTGC CACGAACGTG 
CATCGCAGCG GACCAACATT CATACCGGAT GAGGCACGCA AGGGCTACAC GTTCGATGTG 
GGCAAATTTC GGGACGCCGT TGTAATGCCG TGGTATCGGA ACCGCGACCA GCCGCAGTAC 
TTTTACGTGG CGGAAATATG CAACCATCTG TCGCCGAAGA GCACCTTTCC CGGGTCGAAC 
TACGCGACGT TCGAGGAGTA TTACCATCGC AAGTACAAAA TCCACATTCA GAACCAACGC 
CAGCCGCTGC TGGACGTTGA CCATACCAGT GCGCGGCTAA ACTTCCTGAC GCCCCGTTAC 
GTTAACCGGA AGGGTGTAGC GTTGCCGACC AGCTCGGAGG AGACGAAGCG GGCCAAGCGT 
GAAAACTTGG AGCAGAAACA GATCCTCGTG CCGGAGCTGT GCACGATACA TCCGTTCCCT 
GCCTCGCTGT GGCGTGCGGC CGTCTGCCTG CCGTGCGTAC TGTACCGCAT CAACGCGCTG 
CTGCTGGCAG ATGAGATACG GCGGCAGGTG GCACGCGACC TGCGGCTGGG ATGGGAAAAT 
GTGGACGAGC TGCAGGAGGG CCAGTTCCAG TGGCCAATGC TAAGCTTTGG CTGGAATCTG 
GCCGATGTGC TGCGCAAAAC GAAGGAGCAG AAAATTGCGC AAGCTCAGGA AGCGATCGAT 
GCCAGTGCGC CGGAAGTGGA GGATGAAGTG GAGCTAGACA AGGAAGCGCC GAACGTGCGT 
GATGCTGCGG AGGTTGATGA GGAAGATGGG CTAAAAATGG AAAACGGTGT AATTGCTGAG 
GTTGAAAAGA GTCAGGTGGA TGGAGAGGAT GATACTGGCG ATAAAAAGAC TGATAGCGAT 
GGAACGCTGC TTGAAATTGG TACATGGTCG AACGAAATGG CGGTAGGCGT TGGAACCGAT 
AACGATATGG GTGAAGAAGG TAGGAGAGGC GCATCGTCAC CCTCGTTCCT GCGGTACGAT 
TCCGACTGTT CCAGCAACAG CAGTGCCAAT TTCTACTCTT CGGACGAGTA CGACGAGGAG 
GACGATTACT ACCTGTACGA TGGTTCGGAA AAGCCGAAAA ATGCGATAGA ACCGAGCCAG 
GAGGCAGTAT CGGGCACGGA CAATGCGAAC GACTCGGGCG ATAAGCCGGG CAGTAGGAAC 
CGGACCATCA CGCAGTCACA GGACACGGTG GTAAATCTGG GTGGGCGGTT GAAGATCGAG 
TTCAAGTCGG AAACCGATGC CGAAGCGATC GATTCCGAGT GTGATCTGCA GCGGCAGCGA 
ACGCAGCAGA GCATCATTGA GCGGTCACGC CAGAACGATA TGCTCTATCA GAGCTCGAAA 
AACGCGGTCG ATGGGTTCTG CTACTCGCCG AGCGATCGGC AGTGCGCCGA GGAGCGGGAG 
CAAGCGGAGC AACGGTTTGA GCGGCAGAAA AATCACACCA AAGACACCAT CCGCCAGCAG 
GGCAGCTTGG TGCGATGGAA CGAACCGCTG TCCGTAAGCC ACTGGCGATC GAAGCTGGAC 
GAGTCGGAAG CGGCCACCCT TGGCGCACTG ATGGACGATT TGGGCGGGCA ACCGTTCACG 
GAGCTCGTAC CGTACGTCGA GCCGGAGCAG CTGTTCGAGC TGCTTGTGCG AAACGGTAGC 
AGCGGGGAAC GATGGCTCCG ATTGCACGAT CTCTACTGCT TGAATCGACC GTTCTTCCCG 
GAACGGTACA CCATCTTCCG GGGCGGTTCA CAGTTTGATA ACTTTCTGGA CGAAAGCTGC 
CAGGAAGGTG AGGGTGCTAG TTCGCCACCG AAAGTGATCG AGCTGACTAT TTGCGATCCT 
TTTCCGGCGA TAGCCGCCGG GCAGATGGCT TGCAACTACA AACCGAAACA ACATTCCATG 
CGGAGCGACG AAGCGAACAA GAACGGCCAC GACAGTGCGG GTGTGCTCAG TACGACAGAT 
GAGGCCGGGG GTGTTGATTA CTTCAGTTTC GATTATCAGC CAGATTTGAG CCAACATCCC 
GGTCCCAGTC CGGCTATTAT TCTACAAGCG CTAACCATGT CGAACGCCAA CGATGGCATC 
AACCTGGAGC GATTGGAAAC GATTGGCGAT TCCTTTCTGA AGTACGCCAT CACCACCTAC 
CTTTACTGCC GGTACGACAA CGTGCACGAG GGCAAGCTAA GCCACCTGCG GTCGAAGCAG 
GTATCCAATC TGAACCTTTA CCGGTTGGGG CGCAGGAAGC GGTTGGGCGA CTGCATGATT 
GCGGCCAAAT TTGAACCACA CGATAACTGG CTGCCACCCT GCTACTACGT GCCGAAGGAG 
CTAGAACAAA CGTTGATTGA TGCAAAGATC CCTGCTTGCC ATTGGAATCT GGCCGATCTT 
CCAGACATCA AGCGTCTGTC GTGCGCTGAA ATCTGTCAAC TAGTCAAGGA ACGTGCACGG 
GCCAAACGCA GAGAGGATGT CGACCGGTTC GACCTACAAC ATGCAGACAG CAACGGTGGT 
GAGGACGGAA ATGAAGACGA CGACGATGAC GACGACGACG ATGAAGACGA TACAACCACT 
GGCGAAGGGA ACGAGCACGA AGCGGACAAT GGGTCCTGCT ACATCCCGTA CAATTTAGTA 
ACGCAGCACA GCATCCCCGA CAAATCGGTG GCTGACTGTG TGGAAGCCCT GATCGGTGCC 
TACCTCATCG AATGTGGCCC CCGCGGAGCG CTCCTCTTTA TGGCATGGCT TGGCATACGG 
GTGCTCCCCA TCCGTGAGCC GCCGGTGAAG CTTAATTCAA ACAACGAGAC GGCGCTTACT 
CCTTACAAAG CTACTGGACA GAACGATGGA CCCCTGTCCA CTGGCGTAAC GATTGCTGAG 
TATGGTCACT GGGTTGCACC GCCCTCGCCC ATGGTGCGGG CCAACATTAC GTTCGGTGGC 
ATCGAAACCG GTGCCGCGGC AACGTCCCGC GAGCTGGCCC GGCTGCTGCA AGGGTTCGAG 
GAGTTCGAGC AGGCGCTTGG CTACCGCTTC CGGGACCGTT CCTACCTGCT GCAAGCCATG 
ACGCACGCTT CGTACAGCCC GAACCGGCTG ACGGATTGCT ACCAGCGGCT GGAGTTTCTG 
GGCGATGCCA TCCTCGACTA CCTCATCACG CGCCATCTGT ACGAGGACCG CCGGCAGCAT 
TCACCGGGCG CGCTCACAGA CTTGCGGTCG GCGCTGGTGA ACAATACCAT CTTTGCTTCG 
CTCGCCGTAC GCCACGGATT TCATAAGTAC TTCCTGCACC TGTCGCCCGG GCTGCAGGAG 
GTGATCGATC GATTCGTTCG CATCCAGCAA GAGAACGGGC ATCGCATCAC GGAGGAGGAA 
TACTACCTGC CGGACGAAGA TGACGAGCTG GGCGAGTACG GTGCGATGGG GGAGGACGGT 
CCCGGCGAGG GCCGCGGTGT GGGGGAGGCG GAAGATGTGG AAGTGCCGAA AGCGTTGGGC 
GACGTGTTCG AATCGATTGC CGGTGCCATT TTCCTCGATT CCGACATGTC GCTCGATACG 
GTGTGGAAGG TGTATCGGAA AATGATGGGA CCGGAAATAG AGAAATTCAG CAGCTCGGTC 
CCAAAATCCC CGATACGCGA ACTGCTCGAA ATGGAACCGG AGACGGCCAA ATTTGGCAAA 
CCGGAGAAGC TGACGGACGG ACGGCGCGTG CGGGTAACGG TGGAAGTGTT CGGCAAAGGT 
ACATTCCGTG GCATTGGAAG GAACTATCGC ATCGCCAAGT GTACGGCGGC AAAGTGTGCC 
CTTCGCCAGC TCAAGAAGCT CGGCTATGCC AACCATCACA AGCGCCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Anopheles gambiae genome: an update.
Trends in parasitology20(2)49-52(2004 Feb)
Mongin E,Louis C,Holt RA,Birney E,Collins FH

The genome sequence of the malaria mosquito Anopheles gambiae.
Science (New York, N.Y.)298(5591)129-49(2002 Oct)
Holt RA,Subramanian GM,Halpern A,Sutton GG,Charlab R,Nusskern DR,Wincker P,Clark AG,Ribeiro JM,Wides R,Salzberg SL,Loftus B,Yandell M,Majoros WH,Rusch DB,Lai Z,Kraft CL,Abril JF,Anthouard V,Arensburger P,Atkinson PW,Baden H,de Berardinis V,Baldwin D,Benes V,Biedler J,Blass C,Bolanos R,Boscus D,Barnstead M,Cai S,Center A,Chaturverdi K,Christophides GK,Chrystal MA,Clamp M,Cravchik A,Curwen V,Dana A,Delcher A,Dew I,Evans CA,Flanigan M,Grundschober-Freimoser A,Friedli L,Gu Z,Guan P,Guigo R,Hillenmeyer ME,Hladun SL,Hogan JR,Hong YS,Hoover J,Jaillon O,Ke Z,Kodira C,Kokoza E,Koutsos A,Letunic I,Levitsky A,Liang Y,Lin JJ,Lobo NF,Lopez JR,Malek JA,McIntosh TC,Meister S,Miller J,Mobarry C,Mongin E,Murphy SD,O'Brochta DA,Pfannkoch C,Qi R,Regier MA,Remington K,Shao H,Sharakhova MV,Sitter CD,Shetty J,Smith TJ,Strong R,Sun J,Thomasova D,Ton LQ,Topalis P,Tu Z,Unger MF,Walenz B,Wang A,Wang J,Wang M,Wang X,Woodford KJ,Wortman JR,Wu M,Yao A,Zdobnov EM,Zhang H,Zhao Q,Zhao S,Zhu SC,Zhimulev I,Coluzzi M,della Torre A,Roth CW,Louis C,Kalush F,Mural RJ,Myers EW,Adams MD,Smith HO,Broder S,Gardner MJ,Fraser CM,Birney E,Bork P,Brey PT,Venter JC,Weissenbach J,Kafatos FC,Collins FH,Hoffman SL

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AgaP_AGAP002836 cDNA ORF clone, Anopheles gambiae str. PEST

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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