DICER1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011536600.2	XP_011534902.1	endoribonuclease Dicer isoform X1
XM_011536602.2	XP_011534904.1	endoribonuclease Dicer isoform X1
XM_011536599.1	XP_011534901.1	endoribonuclease Dicer isoform X1
XM_017021121.1	XP_016876610.1	endoribonuclease Dicer isoform X1
XM_011536599.2	XP_011534901.1	endoribonuclease Dicer isoform X1
NM_177438.2	NP_803187.1	endoribonuclease Dicer isoform 1
XM_017021120.1	XP_016876609.1	endoribonuclease Dicer isoform X1
XM_011536604.2	XP_011534906.1	endoribonuclease Dicer isoform X2
XM_017021123.1	XP_016876612.1	endoribonuclease Dicer isoform X2
XM_011536600.3	XP_011534902.1	endoribonuclease Dicer isoform X1
XM_017021123.2	XP_016876612.1	endoribonuclease Dicer isoform X2
NM_001271282.2	NP_001258211.1	endoribonuclease Dicer isoform 1
XM_011536601.2	XP_011534903.1	endoribonuclease Dicer isoform X1
XM_017021120.2	XP_016876609.1	endoribonuclease Dicer isoform X1
XM_011536602.3	XP_011534904.1	endoribonuclease Dicer isoform X1
XM_017021121.2	XP_016876610.1	endoribonuclease Dicer isoform X1
NM_001195573.1	NP_001182502.1	endoribonuclease Dicer isoform 2
NM_001291628.1	NP_001278557.1	endoribonuclease Dicer isoform 1
NM_030621.4	NP_085124.2	endoribonuclease Dicer isoform 1
XM_011536602.1	XP_011534904.1	endoribonuclease Dicer isoform X1
XM_011536603.1	XP_011534905.1	endoribonuclease Dicer isoform X1
XM_011536601.1	XP_011534903.1	endoribonuclease Dicer isoform X1
XM_011536600.1	XP_011534902.1	endoribonuclease Dicer isoform X1
XM_017021122.1	XP_016876611.1	endoribonuclease Dicer isoform X2
XM_011536601.3	XP_011534903.1	endoribonuclease Dicer isoform X1
XM_017021122.2	XP_016876611.1	endoribonuclease Dicer isoform X2
XM_011536605.2	XP_011534907.1	endoribonuclease Dicer isoform X3
NM_001271282.3	NP_001258211.1	endoribonuclease Dicer isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23464	NM_177438.2 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	NM_030621.4 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	NM_001271282.3 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	NM_001291628.1 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_011536600.3 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_011536601.3 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_011536599.2 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_017021120.2 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_017021121.2 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_011536603.1 Latest version!	Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_011536602.3 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu66151	XM_011536605.2 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OHu22176	NM_001195573.1 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu66150	XM_017021123.2 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu66150	XM_017021122.2 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu66150	XM_011536604.2 Latest version!	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu23464	XM_011536599.1	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_011536600.2	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu66150	XM_017021123.1	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu23464	XM_017021120.1	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_011536602.2	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_017021121.1	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_011536602.1	Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_011536601.1	Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_011536600.1	Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu23464	XM_011536601.2	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu66150	XM_017021122.1	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu23464	NM_001271282.2	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
Hide Old Accession Versions >>

DICER1 ( NM_177438.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_803187.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	NM_177438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC150287.1, AL390254.6 and AF007142.1. This sequence is a reference standard in the RefSeqGene project. On Feb 23, 2008 this sequence version replaced NM_177438.1. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Variants 1, 2, 4 and 5 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150287.1, SRR1660803.3388.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_803187.1
CDS	239..6007
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	NM_177438.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177438.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( NM_001271282.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258211.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 4, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	NM_001271282.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ132261.2, AL390254.6 and AF007142.1. On Apr 23, 2014 this sequence version replaced NM_001271282.1. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 2. Variants 1, 2, 4 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ132261.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258211.1
CDS	234..6002
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	NM_001271282.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271282.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( NM_001291628.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278557.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 5, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	NM_001291628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY845868.1, BM152875.1, AJ132261.2, AL390254.6 and AF007142.1. Transcript Variant: This variant (5) lacks an internal segment in the 5' UTR, compared to variant 2. Variants 1, 2, 4 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1660803.189586.1, SRR1803614.213440.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278557.1
CDS	122..5890
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	NM_001291628.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291628.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( NM_030621.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_085124.2 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 2, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	NM_030621.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY845867.1, BM152875.1, AJ132261.2, AL390254.6 and AF007142.1. On Apr 23, 2014 this sequence version replaced NM_030621.3. Transcript Variant: This variant (2) represents the longest transcript. Variants 1, 2, 4 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1803614.25876.1, SRR1660803.189586.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 15987463 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085124.2
CDS	293..6061
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	NM_030621.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030621.4

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( NM_001271282.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258211.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 4, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	NM_001271282.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ132261.2, AL390254.6 and AF007142.1. On Jun 2, 2019 this sequence version replaced NM_001271282.2. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 2. Variants 1, 2, 4 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ132261.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258211.1
CDS	225..5993
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	NM_001271282.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271282.3

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536600.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534902.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_011536600.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536600.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534902.1
CDS	300..6068
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536600.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536600.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536602.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534904.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X5, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_011536602.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534904.1
CDS	336..6104
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536602.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536602.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536599.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534901.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_011536599.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534901.1
CDS	425..6193
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536599.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536599.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_017021121.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876610.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X6, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_017021121.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876610.1
CDS	127..5895
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_017021121.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021121.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536599.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534901.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_011536599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011536599.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534901.1
CDS	1247..7015
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536599.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536599.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_017021120.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876609.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_017021120.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876609.1
CDS	732..6500
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_017021120.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021120.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536600.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534902.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_011536600.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011536600.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534902.1
CDS	1086..6854
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536600.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536600.3

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536601.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534903.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X4, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_011536601.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536601.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534903.1
CDS	445..6213
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536601.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536601.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_017021120.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876609.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_017021120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876609.1
CDS	637..6405
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_017021120.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021120.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536602.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534904.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X5, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_011536602.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011536602.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534904.1
CDS	335..6103
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536602.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536602.3

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_017021121.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876610.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X6, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_017021121.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021121.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876610.1
CDS	919..6687
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_017021121.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021121.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536602.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534904.1 Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant X4, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_011536602.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	endoribonuclease Dicer isoform X1
Comment

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011534904.1

Translation

MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536602.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536602.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536603.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534905.1 Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant X5, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_011536603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	endoribonuclease Dicer isoform X1
Comment

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011534905.1

Translation

MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536603.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536603.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536601.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534903.1 Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_011536601.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	endoribonuclease Dicer isoform X1
Comment

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011534903.1

Translation

MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536601.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536601.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536600.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534902.1 Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant X2, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_011536600.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	endoribonuclease Dicer isoform X1
Comment

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011534902.1

Translation

MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536600.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens dicer 1, ribonuclease type III (DICER1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536600.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536601.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534903.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X4, mRNA.

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CloneID	OHu23464
Clone ID Related Accession (Same CDS sequence)	XM_011536600.2 , XM_011536602.2 , XM_011536599.1 , XM_017021121.1 , XM_011536599.2 , NM_177438.2 , XM_017021120.1 , XM_011536600.3 , NM_001271282.2 , XM_011536601.2 , XM_017021120.2 , XM_011536602.3 , XM_017021121.2 , NM_001291628.1 , NM_030621.4 , XM_011536602.1 , XM_011536603.1 , XM_011536601.1 , XM_011536600.1 , XM_011536601.3 , NM_001271282.3
Accession Version	XM_011536601.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011536601.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534903.1
CDS	444..6212
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536601.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536601.3

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCTGAGG AGGATGAAGA GAAAGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTTGAGT CGCTTGCTGG TGCCATTTAC 
ATGGATAGTG GGATGTCACT GGAGACAGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCA 
CTAATAGAAA AGTTTTCTGC AAATGTACCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT TAGCCCGGCT GAGAGAACTT ACGACGGGAA GGTCAGAGTC 
ACTGTGGAAG TAGTAGGAAA GGGGAAATTT AAAGGTGTTG GTCGAAGTTA CAGGATTGCC 
AAATCTGCAG CAGCAAGAAG AGCCCTCCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536605.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534907.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X10, mRNA.

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CloneID	OHu66151
Clone ID Related Accession (Same CDS sequence)	XM_011536605.2
Accession Version	XM_011536605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCAG AATTCAGAAA ACAGGAAGAG GTACTTAGGA AATTTCGAGC ACATGAGACC 
AACCTGCTTA TTGCAACAAG TATTGTAGAA GAGGGTGTTG ATATACCAAA ATGCAACTTG 
GTGGTTCGTT TTGATTTGCC CACAGAATAT CGATCCTATG TTCAATCTAA AGGAAGAGCA 
AGGGCACCCA TCTCTAATTA TATAATGTTA GCGGATACAG ACAAAATAAA AAGTTTTGAA 
GAAGACCTTA AAACCTACAA AGCTATTGAA AAGATCTTGA GAAACAAGTG TTCCAAGTCG 
GTTGATACTG GTGAGACTGA CATTGATCCT GTCATGGATG ATGATGACGT TTTCCCACCA 
TATGTGTTGA GGCCTGACGA TGGTGGTCCA CGAGTCACAA TCAACACGGC CATTGGACAC 
ATCAATAGAT ACTGTGCTAG ATTACCAAGT GATCCGTTTA CTCATCTAGC TCCTAAATGC 
AGAACCCGAG AGTTGCCTGA TGGTACATTT TATTCAACTC TTTATCTGCC AATTAACTCA 
CCTCTTCGAG CCTCCATTGT TGGTCCACCA ATGAGCTGTG TACGATTGGC TGAAAGAGTT 
GTAGCTCTCA TTTGCTGTGA GAAACTGCAC AAAATTGGCG AACTGGATGA CCATTTGATG 
CCAGTTGGGA AAGAGACTGT TAAATATGAA GAGGAGCTTG ATTTGCATGA TGAAGAAGAG 
ACCAGTGTTC CAGGAAGACC AGGTTCCACG AAACGAAGGC AGTGCTACCC AAAAGCAATT 
CCAGAGTGTT TGAGGGATAG TTATCCCAGA CCTGATCAGC CCTGTTACCT GTATGTGATA 
GGAATGGTTT TAACTACACC TTTACCTGAT GAACTCAACT TTAGAAGGCG GAAGCTCTAT 
CCTCCTGAAG ATACCACAAG ATGCTTTGGA ATACTGACGG CCAAACCCAT ACCTCAGATT 
CCACACTTTC CTGTGTACAC ACGCTCTGGA GAGGTTACCA TATCCATTGA GTTGAAGAAG 
TCTGGTTTCA TGTTGTCTCT ACAAATGCTT GAGTTGATTA CAAGACTTCA CCAGTATATA 
TTCTCACATA TTCTTCGGCT TGAAAAACCT GCACTAGAAT TTAAACCTAC AGACGCTGAT 
TCAGCATACT GTGTTCTACC TCTTAATGTT GTTAATGACT CCAGCACTTT GGATATTGAC 
TTTAAATTCA TGGAAGATAT TGAGAAGTCT GAAGCTCGCA TAGGCATTCC CAGTACAAAG 
TATACAAAAG AAACACCCTT TGTTTTTAAA TTAGAAGATT ACCAAGATGC CGTTATCATT 
CCAAGATATC GCAATTTTGA TCAGCCTCAT CGATTTTATG TAGCTGATGT GTACACTGAT 
CTTACCCCAC TCAGTAAATT TCCTTCCCCT GAGTATGAAA CTTTTGCAGA ATATTATAAA 
ACAAAGTACA ACCTTGACCT AACCAATCTC AACCAGCCAC TGCTGGATGT GGACCACACA 
TCTTCAAGAC TTAATCTTTT GACACCTCGA CATTTGAATC AGAAGGGGAA AGCGCTTCCT 
TTAAGCAGTG CTGAGAAGAG GAAAGCCAAA TGGGAAAGTC TGCAGAATAA ACAGATACTG 
GTTCCAGAAC TCTGTGCTAT ACATCCAATT CCAGCATCAC TGTGGAGAAA AGCTGTTTGT 
CTCCCCAGCA TACTTTATCG CCTTCACTGC CTTTTGACTG CAGAGGAGCT AAGAGCCCAG 
ACTGCCAGCG ATGCTGGCGT GGGAGTCAGA TCACTTCCTG CGGATTTTAG ATACCCTAAC 
TTAGACTTCG GGTGGAAAAA ATCTATTGAC AGCAAATCTT TCATCTCAAT TTCTAACTCC 
TCTTCAGCTG AAAATGATAA TTACTGTAAG CACAGCACAA TTGTCCCTGA AAATGCTGCA 
CATCAAGGTG CTAATAGAAC CTCCTCTCTA GAAAATCATG ACCAAATGTC TGTGAACTGC 
AGAACGTTGC TCAGCGAGTC CCCTGGTAAG CTCCACGTTG AAGTTTCAGC AGATCTTACA 
GCAATTAATG GTCTTTCTTA CAATCAAAAT CTCGCCAATG GCAGTTATGA TTTAGCTAAC 
AGAGACTTTT GCCAAGGAAA TCAGCTAAAT TACTACAAGC AGGAAATACC CGTGCAACCA 
ACTACCTCAT ATTCCATTCA GAATTTATAC AGTTACGAGA ACCAGCCCCA GCCCAGCGAT 
GAATGTACTC TCCTGAGTAA TAAATACCTT GATGGAAATG CTAACAAATC TACCTCAGAT 
GGAAGTCCTG TGATGGCCGT AATGCCTGGT ACGACAGACA CTATTCAAGT GCTCAAGGGC 
AGGATGGATT CTGAGCAGAG CCCTTCTATT GGGTACTCCT CAAGGACTCT TGGCCCCAAT 
CCTGGACTTA TTCTTCAGGC TTTGACTCTG TCAAACGCTA GTGATGGATT TAACCTGGAG 
CGGCTTGAAA TGCTTGGCGA CTCCTTTTTA AAGCATGCCA TCACCACATA TCTATTTTGC 
ACTTACCCTG ATGCGCATGA GGGCCGCCTT TCATATATGA GAAGCAAAAA GGTCAGCAAC 
TGTAATCTGT ATCGCCTTGG AAAAAAGAAG GGACTACCCA GCCGCATGGT GGTGTCAATA 
TTTGATCCCC CTGTGAATTG GCTTCCTCCT GGTTATGTAG TAAATCAAGA CAAAAGCAAC 
ACAGATAAAT GGGAAAAAGA TGAAATGACA AAAGACTGCA TGCTGGCGAA TGGCAAACTG 
GATGAGGATT ACGAGGAGGA GGATGAGGAG GAGGAGAGCC TGATGTGGAG GGCTCCGAAG 
GAAGAGGCTG ACTATGAAGA TGATTTCCTG GAGTATGATC AGGAACATAT CAGATTTATA 
GATAATATGT TAATGGGGTC AGGAGCTTTT GTAAAGAAAA TCTCTCTTTC TCCTTTTTCA 
ACCACTGATT CTGCATATGA ATGGAAAATG CCCAAAAAAT CCTCCTTAGG TAGTATGCCA 
TTTTCATCAG ATTTTGAGGA TTTTGACTAC AGCTCTTGGG ATGCAATGTG CTATCTGGAT 
CCTAGCAAAG CTGTTGAAGA AGATGACTTT GTGGTGGGGT TCTGGAATCC ATCAGAAGAA 
AACTGTGGTG TTGACACGGG AAAGCAGTCC ATTTCTTACG ACTTGCACAC TGAGCAGTGT 
ATTGCTGACA AAAGCATAGC GGACTGTGTG GAAGCCCTGC TGGGCTGCTA TTTAACCAGC 
TGTGGGGAGA GGGCTGCTCA GCTTTTCCTC TGTTCACTGG GGCTGAAGGT GCTCCCGGTA 
ATTAAAAGGA CTGATCGGGA AAAGGCCCTG TGCCCTACTC GGGAGAATTT CAACAGCCAA 
CAAAAGAACC TTTCAGTGAG CTGTGCTGCT GCTTCTGTGG CCAGTTCACG CTCTTCTGTA 
TTGAAAGACT CGGAATATGG TTGTTTGAAG ATTCCACCAA GATGTATGTT TGATCATCCA 
GATGCAGATA AAACACTGAA TCACCTTATA TCGGGGTTTG AAAATTTTGA AAAGAAAATC 
AACTACAGAT TCAAGAATAA GGCTTACCTT CTCCAGGCTT TTACACATGC CTCCTACCAC 
TACAATACTA TCACTGATTG TTACCAGCGC TTAGAATTCC TGGGAGATGC GATTTTGGAC 
TACCTCATAA CCAAGCACCT TTATGAAGAC CCGCGGCAGC ACTCCCCGGG GGTCCTGACA 
GACCTGCGGT CTGCCCTGGT CAACAACACC ATCTTTGCAT CGCTGGCTGT AAAGTACGAC 
TACCACAAGT ACTTCAAAGC TGTCTCTCCT GAGCTCTTCC ATGTCATTGA TGACTTTGTG 
CAGTTTCAGC TTGAGAAGAA TGAAATGCAA GGAATGGATT CTGAGCTTAG GAGATCTGAG 
GAGGATGAAG AGAAAGAAGA GGATATTGAA GTTCCAAAGG CCATGGGGGA TATTTTTGAG 
TCGCTTGCTG GTGCCATTTA CATGGATAGT GGGATGTCAC TGGAGACAGT CTGGCAGGTG 
TACTATCCCA TGATGCGGCC ACTAATAGAA AAGTTTTCTG CAAATGTACC CCGTTCCCCT 
GTGCGAGAAT TGCTTGAAAT GGAACCAGAA ACTGCCAAAT TTAGCCCGGC TGAGAGAACT 
TACGACGGGA AGGTCAGAGT CACTGTGGAA GTAGTAGGAA AGGGGAAATT TAAAGGTGTT 
GGTCGAAGTT ACAGGATTGC CAAATCTGCA GCAGCAAGAA GAGCCCTCCG AAGCCTCAAA 
GCTAATCAAC CTCAGGTTCC CAATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534907.1
CDS	6405..10694
Translation	MEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536605.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536605.2

ORF Insert Sequence:

ATGGAAGCAG AATTCAGAAA ACAGGAAGAG GTACTTAGGA AATTTCGAGC ACATGAGACC 
AACCTGCTTA TTGCAACAAG TATTGTAGAA GAGGGTGTTG ATATACCAAA ATGCAACTTG 
GTGGTTCGTT TTGATTTGCC CACAGAATAT CGATCCTATG TTCAATCTAA AGGAAGAGCA 
AGGGCACCCA TCTCTAATTA TATAATGTTA GCGGATACAG ACAAAATAAA AAGTTTTGAA 
GAAGACCTTA AAACCTACAA AGCTATTGAA AAGATCTTGA GAAACAAGTG TTCCAAGTCG 
GTTGATACTG GTGAGACTGA CATTGATCCT GTCATGGATG ATGATGACGT TTTCCCACCA 
TATGTGTTGA GGCCTGACGA TGGTGGTCCA CGAGTCACAA TCAACACGGC CATTGGACAC 
ATCAATAGAT ACTGTGCTAG ATTACCAAGT GATCCGTTTA CTCATCTAGC TCCTAAATGC 
AGAACCCGAG AGTTGCCTGA TGGTACATTT TATTCAACTC TTTATCTGCC AATTAACTCA 
CCTCTTCGAG CCTCCATTGT TGGTCCACCA ATGAGCTGTG TACGATTGGC TGAAAGAGTT 
GTAGCTCTCA TTTGCTGTGA GAAACTGCAC AAAATTGGCG AACTGGATGA CCATTTGATG 
CCAGTTGGGA AAGAGACTGT TAAATATGAA GAGGAGCTTG ATTTGCATGA TGAAGAAGAG 
ACCAGTGTTC CAGGAAGACC AGGTTCCACG AAACGAAGGC AGTGCTACCC AAAAGCAATT 
CCAGAGTGTT TGAGGGATAG TTATCCCAGA CCTGATCAGC CCTGTTACCT GTATGTGATA 
GGAATGGTTT TAACTACACC TTTACCTGAT GAACTCAACT TTAGAAGGCG GAAGCTCTAT 
CCTCCTGAAG ATACCACAAG ATGCTTTGGA ATACTGACGG CCAAACCCAT ACCTCAGATT 
CCACACTTTC CTGTGTACAC ACGCTCTGGA GAGGTTACCA TATCCATTGA GTTGAAGAAG 
TCTGGTTTCA TGTTGTCTCT ACAAATGCTT GAGTTGATTA CAAGACTTCA CCAGTATATA 
TTCTCACATA TTCTTCGGCT TGAAAAACCT GCACTAGAAT TTAAACCTAC AGACGCTGAT 
TCAGCATACT GTGTTCTACC TCTTAATGTT GTTAATGACT CCAGCACTTT GGATATTGAC 
TTTAAATTCA TGGAAGATAT TGAGAAGTCT GAAGCTCGCA TAGGCATTCC CAGTACAAAG 
TATACAAAAG AAACACCCTT TGTTTTTAAA TTAGAAGATT ACCAAGATGC CGTTATCATT 
CCAAGATATC GCAATTTTGA TCAGCCTCAT CGATTTTATG TAGCTGATGT GTACACTGAT 
CTTACCCCAC TCAGTAAATT TCCTTCCCCT GAGTATGAAA CTTTTGCAGA ATATTATAAA 
ACAAAGTACA ACCTTGACCT AACCAATCTC AACCAGCCAC TGCTGGATGT GGACCACACA 
TCTTCAAGAC TTAATCTTTT GACACCTCGA CATTTGAATC AGAAGGGGAA AGCGCTTCCT 
TTAAGCAGTG CTGAGAAGAG GAAAGCCAAA TGGGAAAGTC TGCAGAATAA ACAGATACTG 
GTTCCAGAAC TCTGTGCTAT ACATCCAATT CCAGCATCAC TGTGGAGAAA AGCTGTTTGT 
CTCCCCAGCA TACTTTATCG CCTTCACTGC CTTTTGACTG CAGAGGAGCT AAGAGCCCAG 
ACTGCCAGCG ATGCTGGCGT GGGAGTCAGA TCACTTCCTG CGGATTTTAG ATACCCTAAC 
TTAGACTTCG GGTGGAAAAA ATCTATTGAC AGCAAATCTT TCATCTCAAT TTCTAACTCC 
TCTTCAGCTG AAAATGATAA TTACTGTAAG CACAGCACAA TTGTCCCTGA AAATGCTGCA 
CATCAAGGTG CTAATAGAAC CTCCTCTCTA GAAAATCATG ACCAAATGTC TGTGAACTGC 
AGAACGTTGC TCAGCGAGTC CCCTGGTAAG CTCCACGTTG AAGTTTCAGC AGATCTTACA 
GCAATTAATG GTCTTTCTTA CAATCAAAAT CTCGCCAATG GCAGTTATGA TTTAGCTAAC 
AGAGACTTTT GCCAAGGAAA TCAGCTAAAT TACTACAAGC AGGAAATACC CGTGCAACCA 
ACTACCTCAT ATTCCATTCA GAATTTATAC AGTTACGAGA ACCAGCCCCA GCCCAGCGAT 
GAATGTACTC TCCTGAGTAA TAAATACCTT GATGGAAATG CTAACAAATC TACCTCAGAT 
GGAAGTCCTG TGATGGCCGT AATGCCTGGT ACGACAGACA CTATTCAAGT GCTCAAGGGC 
AGGATGGATT CTGAGCAGAG CCCTTCTATT GGGTACTCCT CAAGGACTCT TGGCCCCAAT 
CCTGGACTTA TTCTTCAGGC TTTGACTCTG TCAAACGCTA GTGATGGATT TAACCTGGAG 
CGGCTTGAAA TGCTTGGCGA CTCCTTTTTA AAGCATGCCA TCACCACATA TCTATTTTGC 
ACTTACCCTG ATGCGCATGA GGGCCGCCTT TCATATATGA GAAGCAAAAA GGTCAGCAAC 
TGTAATCTGT ATCGCCTTGG AAAAAAGAAG GGACTACCCA GCCGCATGGT GGTGTCAATA 
TTTGATCCCC CTGTGAATTG GCTTCCTCCT GGTTATGTAG TAAATCAAGA CAAAAGCAAC 
ACAGATAAAT GGGAAAAAGA TGAAATGACA AAAGACTGCA TGCTGGCGAA TGGCAAACTG 
GATGAGGATT ACGAGGAGGA GGATGAGGAG GAGGAGAGCC TGATGTGGAG GGCTCCGAAG 
GAAGAGGCTG ACTATGAAGA TGATTTCCTG GAGTATGATC AGGAACATAT CAGATTTATA 
GATAATATGT TAATGGGGTC AGGAGCTTTT GTAAAGAAAA TCTCTCTTTC TCCTTTTTCA 
ACCACTGATT CTGCATATGA ATGGAAAATG CCCAAAAAAT CCTCCTTAGG TAGTATGCCA 
TTTTCATCAG ATTTTGAGGA TTTTGACTAC AGCTCTTGGG ATGCAATGTG CTATCTGGAT 
CCTAGCAAAG CTGTTGAAGA AGATGACTTT GTGGTGGGGT TCTGGAATCC ATCAGAAGAA 
AACTGTGGTG TTGACACGGG AAAGCAGTCC ATTTCTTACG ACTTGCACAC TGAGCAGTGT 
ATTGCTGACA AAAGCATAGC GGACTGTGTG GAAGCCCTGC TGGGCTGCTA TTTAACCAGC 
TGTGGGGAGA GGGCTGCTCA GCTTTTCCTC TGTTCACTGG GGCTGAAGGT GCTCCCGGTA 
ATTAAAAGGA CTGATCGGGA AAAGGCCCTG TGCCCTACTC GGGAGAATTT CAACAGCCAA 
CAAAAGAACC TTTCAGTGAG CTGTGCTGCT GCTTCTGTGG CCAGTTCACG CTCTTCTGTA 
TTGAAAGACT CGGAATATGG TTGTTTGAAG ATTCCACCAA GATGTATGTT TGATCATCCA 
GATGCAGATA AAACACTGAA TCACCTTATA TCGGGGTTTG AAAATTTTGA AAAGAAAATC 
AACTACAGAT TCAAGAATAA GGCTTACCTT CTCCAGGCTT TTACACATGC CTCCTACCAC 
TACAATACTA TCACTGATTG TTACCAGCGC TTAGAATTCC TGGGAGATGC GATTTTGGAC 
TACCTCATAA CCAAGCACCT TTATGAAGAC CCGCGGCAGC ACTCCCCGGG GGTCCTGACA 
GACCTGCGGT CTGCCCTGGT CAACAACACC ATCTTTGCAT CGCTGGCTGT AAAGTACGAC 
TACCACAAGT ACTTCAAAGC TGTCTCTCCT GAGCTCTTCC ATGTCATTGA TGACTTTGTG 
CAGTTTCAGC TTGAGAAGAA TGAAATGCAA GGAATGGATT CTGAGCTTAG GAGATCTGAG 
GAGGATGAAG AGAAAGAAGA GGATATTGAA GTTCCAAAGG CCATGGGGGA TATTTTTGAG 
TCGCTTGCTG GTGCCATTTA CATGGATAGT GGGATGTCAC TGGAGACAGT CTGGCAGGTG 
TACTATCCCA TGATGCGGCC ACTAATAGAA AAGTTTTCTG CAAATGTACC CCGTTCCCCT 
GTGCGAGAAT TGCTTGAAAT GGAACCAGAA ACTGCCAAAT TTAGCCCGGC TGAGAGAACT 
TACGACGGGA AGGTCAGAGT CACTGTGGAA GTAGTAGGAA AGGGGAAATT TAAAGGTGTT 
GGTCGAAGTT ACAGGATTGC CAAATCTGCA GCAGCAAGAA GAGCCCTCCG AAGCCTCAAA 
GCTAATCAAC CTCAGGTTCC CAATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( NM_001195573.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182502.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 3, mRNA.

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CloneID	OHu22176
Clone ID Related Accession (Same CDS sequence)	NM_001195573.1
Accession Version	NM_001195573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356017.3, HM595745.1 and AL390254.6. Transcript Variant: This variant (3) lacks an exon in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 2. The resulting isoform 2 (also called t-Dicer) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM595745.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGAAAAGT TTTCTGCAAA TGTACCCCGT TCCCCTGTGC 
GAGAATTGCT TGAAATGGAA CCAGAAACTG CCAAATTTAG CCCGGCTGAG AGAACTTACG 
ACGGGAAGGT CAGAGTCACT GTGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182502.1
CDS	46..5535
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSEKSFLQMYPVPLCENCLKWNQKLPNLARLRELTTGRSESLWK

Target ORF information:

RefSeq Version	NM_001195573.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195573.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATCGTCTGTT TAAACACTGG CTCAGGGAAG ACATTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAGC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAG ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTATCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCATGTCCT 
CGCATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAAAAGATTC AGAAACTAGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTCTTAG ACAGGTATAC TTCTCAGCCA TGTGAGATTG TGGTGGATTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAAT TAGAAGAAGC ACTTAATTTT 
ATCAATGATT GTAATATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCGAAA 
CAGATACTAT CAGACTGTCG TGCCGTATTG GTAGTTCTGG GACCCTGGTG TGCAGATAAA 
GTAGCTGGAA TGATGGTAAG AGAACTACAG AAATACATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATCAAACTG 
CTCGAAATCT TACGCAAATA TAAACCATAT GAGCGACAGC AGTTTGAAAG CGTTGAGTGG 
TATAATAATA GAAATCAGGA TAATTATGTG TCATGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACAAATT TTCCTTCTCC TTTTACCAAC 
ATTTTGTGCG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTATA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGGAAGAA TCAGCCTCGC AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTGCTTAT TGCAACAAGT 
ATTGTAGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGTTT TGATTTGCCC 
ACAGAATATC GATCCTATGT TCAATCTAAA GGAAGAGCAA GGGCACCCAT CTCTAATTAT 
ATAATGTTAG CGGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACCTACAAA 
GCTATTGAAA AGATCTTGAG AAACAAGTGT TCCAAGTCGG TTGATACTGG TGAGACTGAC 
ATTGATCCTG TCATGGATGA TGATGACGTT TTCCCACCAT ATGTGTTGAG GCCTGACGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGCA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTTCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCT GAAAGAGTTG TAGCTCTCAT TTGCTGTGAG 
AAACTGCACA AAATTGGCGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACTGTT 
AAATATGAAG AGGAGCTTGA TTTGCATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACGA AACGAAGGCA GTGCTACCCA AAAGCAATTC CAGAGTGTTT GAGGGATAGT 
TATCCCAGAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTTT AACTACACCT 
TTACCTGATG AACTCAACTT TAGAAGGCGG AAGCTCTATC CTCCTGAAGA TACCACAAGA 
TGCTTTGGAA TACTGACGGC CAAACCCATA CCTCAGATTC CACACTTTCC TGTGTACACA 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAT GTTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCTACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TTAATGACTC CAGCACTTTG GATATTGACT TTAAATTCAT GGAAGATATT 
GAGAAGTCTG AAGCTCGCAT AGGCATTCCC AGTACAAAGT ATACAAAAGA AACACCCTTT 
GTTTTTAAAT TAGAAGATTA CCAAGATGCC GTTATCATTC CAAGATATCG CAATTTTGAT 
CAGCCTCATC GATTTTATGT AGCTGATGTG TACACTGATC TTACCCCACT CAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAGTACAA CCTTGACCTA 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGAC ATTTGAATCA GAAGGGGAAA GCGCTTCCTT TAAGCAGTGC TGAGAAGAGG 
AAAGCCAAAT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAACT CTGTGCTATA 
CATCCAATTC CAGCATCACT GTGGAGAAAA GCTGTTTGTC TCCCCAGCAT ACTTTATCGC 
CTTCACTGCC TTTTGACTGC AGAGGAGCTA AGAGCCCAGA CTGCCAGCGA TGCTGGCGTG 
GGAGTCAGAT CACTTCCTGC GGATTTTAGA TACCCTAACT TAGACTTCGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAATT TCTAACTCCT CTTCAGCTGA AAATGATAAT 
TACTGTAAGC ACAGCACAAT TGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCTCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGCT CAGCGAGTCC 
CCTGGTAAGC TCCACGTTGA AGTTTCAGCA GATCTTACAG CAATTAATGG TCTTTCTTAC 
AATCAAAATC TCGCCAATGG CAGTTATGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAT 
CAGCTAAATT ACTACAAGCA GGAAATACCC GTGCAACCAA CTACCTCATA TTCCATTCAG 
AATTTATACA GTTACGAGAA CCAGCCCCAG CCCAGCGATG AATGTACTCT CCTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACAAATCT ACCTCAGATG GAAGTCCTGT GATGGCCGTA 
ATGCCTGGTA CGACAGACAC TATTCAAGTG CTCAAGGGCA GGATGGATTC TGAGCAGAGC 
CCTTCTATTG GGTACTCCTC AAGGACTCTT GGCCCCAATC CTGGACTTAT TCTTCAGGCT 
TTGACTCTGT CAAACGCTAG TGATGGATTT AACCTGGAGC GGCTTGAAAT GCTTGGCGAC 
TCCTTTTTAA AGCATGCCAT CACCACATAT CTATTTTGCA CTTACCCTGA TGCGCATGAG 
GGCCGCCTTT CATATATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGCCTTGGA 
AAAAAGAAGG GACTACCCAG CCGCATGGTG GTGTCAATAT TTGATCCCCC TGTGAATTGG 
CTTCCTCCTG GTTATGTAGT AAATCAAGAC AAAAGCAACA CAGATAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GCTGGCGAAT GGCAAACTGG ATGAGGATTA CGAGGAGGAG 
GATGAGGAGG AGGAGAGCCT GATGTGGAGG GCTCCGAAGG AAGAGGCTGA CTATGAAGAT 
GATTTCCTGG AGTATGATCA GGAACATATC AGATTTATAG ATAATATGTT AATGGGGTCA 
GGAGCTTTTG TAAAGAAAAT CTCTCTTTCT CCTTTTTCAA CCACTGATTC TGCATATGAA 
TGGAAAATGC CCAAAAAATC CTCCTTAGGT AGTATGCCAT TTTCATCAGA TTTTGAGGAT 
TTTGACTACA GCTCTTGGGA TGCAATGTGC TATCTGGATC CTAGCAAAGC TGTTGAAGAA 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT TGACACGGGA 
AAGCAGTCCA TTTCTTACGA CTTGCACACT GAGCAGTGTA TTGCTGACAA AAGCATAGCG 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGGGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCACTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCCTGT GCCCTACTCG GGAGAATTTC AACAGCCAAC AAAAGAACCT TTCAGTGAGC 
TGTGCTGCTG CTTCTGTGGC CAGTTCACGC TCTTCTGTAT TGAAAGACTC GGAATATGGT 
TGTTTGAAGA TTCCACCAAG ATGTATGTTT GATCATCCAG ATGCAGATAA AACACTGAAT 
CACCTTATAT CGGGGTTTGA AAATTTTGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TCCAGGCTTT TACACATGCC TCCTACCACT ACAATACTAT CACTGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCG ATTTTGGACT ACCTCATAAC CAAGCACCTT 
TATGAAGACC CGCGGCAGCA CTCCCCGGGG GTCCTGACAG ACCTGCGGTC TGCCCTGGTC 
AACAACACCA TCTTTGCATC GCTGGCTGTA AAGTACGACT ACCACAAGTA CTTCAAAGCT 
GTCTCTCCTG AGCTCTTCCA TGTCATTGAT GACTTTGTGC AGTTTCAGCT TGAGAAGAAT 
GAAATGCAAG GAATGGATTC TGAGAAAAGT TTTCTGCAAA TGTACCCCGT TCCCCTGTGC 
GAGAATTGCT TGAAATGGAA CCAGAAACTG CCAAATTTAG CCCGGCTGAG AGAACTTACG 
ACGGGAAGGT CAGAGTCACT GTGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_011536604.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534906.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X9, mRNA.

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CloneID	OHu66150
Clone ID Related Accession (Same CDS sequence)	XM_011536604.2 , XM_017021123.1 , XM_017021123.2 , XM_017021122.1 , XM_017021122.2
Accession Version	XM_011536604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536604.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAG GACGGTGTTC TTGGTCAACT CTGGTTCTCA TTATGACTTG CTATGTCGCC 
TTGAATGTTT TGAAAAATGG TTACTTATCA CTGTCAGACA TTAACCTTTT GGTGTTTGAT 
GAGTGTCATC TTGCAATCCT AGACCACCCC TATCGAGAAA TTATGAAGCT CTGTGAAAAT 
TGTCCATCAT GTCCTCGCAT TTTGGGACTA ACTGCTTCCA TTTTAAATGG GAAATGTGAT 
CCAGAGGAAT TGGAAGAAAA GATTCAGAAA CTAGAGAAAA TTCTTAAGAG TAATGCTGAA 
ACTGCAACTG ACCTGGTGGT CTTAGACAGG TATACTTCTC AGCCATGTGA GATTGTGGTG 
GATTGTGGAC CATTTACTGA CAGAAGTGGG CTTTATGAAA GACTGCTGAT GGAATTAGAA 
GAAGCACTTA ATTTTATCAA TGATTGTAAT ATATCTGTAC ATTCAAAAGA AAGAGATTCT 
ACTTTAATTT CGAAACAGAT ACTATCAGAC TGTCGTGCCG TATTGGTAGT TCTGGGACCC 
TGGTGTGCAG ATAAAGTAGC TGGAATGATG GTAAGAGAAC TACAGAAATA CATCAAACAT 
GAGCAAGAGG AGCTGCACAG GAAATTTTTA TTGTTTACAG ACACTTTCCT AAGGAAAATA 
CATGCACTAT GTGAAGAGCA CTTCTCACCT GCCTCACTTG ACCTGAAATT TGTAACTCCT 
AAAGTAATCA AACTGCTCGA AATCTTACGC AAATATAAAC CATATGAGCG ACAGCAGTTT 
GAAAGCGTTG AGTGGTATAA TAATAGAAAT CAGGATAATT ATGTGTCATG GAGTGATTCT 
GAGGATGATG ATGAGGATGA AGAAATTGAA GAAAAAGAGA AGCCAGAGAC AAATTTTCCT 
TCTCCTTTTA CCAACATTTT GTGCGGAATT ATTTTTGTGG AAAGAAGATA CACAGCAGTT 
GTCTTAAACA GATTGATAAA GGAAGCTGGC AAACAAGATC CAGAGCTGGC TTATATCAGT 
AGCAATTTCA TAACTGGACA TGGCATTGGG AAGAATCAGC CTCGCAACAA ACAGATGGAA 
GCAGAATTCA GAAAACAGGA AGAGGTACTT AGGAAATTTC GAGCACATGA GACCAACCTG 
CTTATTGCAA CAAGTATTGT AGAAGAGGGT GTTGATATAC CAAAATGCAA CTTGGTGGTT 
CGTTTTGATT TGCCCACAGA ATATCGATCC TATGTTCAAT CTAAAGGAAG AGCAAGGGCA 
CCCATCTCTA ATTATATAAT GTTAGCGGAT ACAGACAAAA TAAAAAGTTT TGAAGAAGAC 
CTTAAAACCT ACAAAGCTAT TGAAAAGATC TTGAGAAACA AGTGTTCCAA GTCGGTTGAT 
ACTGGTGAGA CTGACATTGA TCCTGTCATG GATGATGATG ACGTTTTCCC ACCATATGTG 
TTGAGGCCTG ACGATGGTGG TCCACGAGTC ACAATCAACA CGGCCATTGG ACACATCAAT 
AGATACTGTG CTAGATTACC AAGTGATCCG TTTACTCATC TAGCTCCTAA ATGCAGAACC 
CGAGAGTTGC CTGATGGTAC ATTTTATTCA ACTCTTTATC TGCCAATTAA CTCACCTCTT 
CGAGCCTCCA TTGTTGGTCC ACCAATGAGC TGTGTACGAT TGGCTGAAAG AGTTGTAGCT 
CTCATTTGCT GTGAGAAACT GCACAAAATT GGCGAACTGG ATGACCATTT GATGCCAGTT 
GGGAAAGAGA CTGTTAAATA TGAAGAGGAG CTTGATTTGC ATGATGAAGA AGAGACCAGT 
GTTCCAGGAA GACCAGGTTC CACGAAACGA AGGCAGTGCT ACCCAAAAGC AATTCCAGAG 
TGTTTGAGGG ATAGTTATCC CAGACCTGAT CAGCCCTGTT ACCTGTATGT GATAGGAATG 
GTTTTAACTA CACCTTTACC TGATGAACTC AACTTTAGAA GGCGGAAGCT CTATCCTCCT 
GAAGATACCA CAAGATGCTT TGGAATACTG ACGGCCAAAC CCATACCTCA GATTCCACAC 
TTTCCTGTGT ACACACGCTC TGGAGAGGTT ACCATATCCA TTGAGTTGAA GAAGTCTGGT 
TTCATGTTGT CTCTACAAAT GCTTGAGTTG ATTACAAGAC TTCACCAGTA TATATTCTCA 
CATATTCTTC GGCTTGAAAA ACCTGCACTA GAATTTAAAC CTACAGACGC TGATTCAGCA 
TACTGTGTTC TACCTCTTAA TGTTGTTAAT GACTCCAGCA CTTTGGATAT TGACTTTAAA 
TTCATGGAAG ATATTGAGAA GTCTGAAGCT CGCATAGGCA TTCCCAGTAC AAAGTATACA 
AAAGAAACAC CCTTTGTTTT TAAATTAGAA GATTACCAAG ATGCCGTTAT CATTCCAAGA 
TATCGCAATT TTGATCAGCC TCATCGATTT TATGTAGCTG ATGTGTACAC TGATCTTACC 
CCACTCAGTA AATTTCCTTC CCCTGAGTAT GAAACTTTTG CAGAATATTA TAAAACAAAG 
TACAACCTTG ACCTAACCAA TCTCAACCAG CCACTGCTGG ATGTGGACCA CACATCTTCA 
AGACTTAATC TTTTGACACC TCGACATTTG AATCAGAAGG GGAAAGCGCT TCCTTTAAGC 
AGTGCTGAGA AGAGGAAAGC CAAATGGGAA AGTCTGCAGA ATAAACAGAT ACTGGTTCCA 
GAACTCTGTG CTATACATCC AATTCCAGCA TCACTGTGGA GAAAAGCTGT TTGTCTCCCC 
AGCATACTTT ATCGCCTTCA CTGCCTTTTG ACTGCAGAGG AGCTAAGAGC CCAGACTGCC 
AGCGATGCTG GCGTGGGAGT CAGATCACTT CCTGCGGATT TTAGATACCC TAACTTAGAC 
TTCGGGTGGA AAAAATCTAT TGACAGCAAA TCTTTCATCT CAATTTCTAA CTCCTCTTCA 
GCTGAAAATG ATAATTACTG TAAGCACAGC ACAATTGTCC CTGAAAATGC TGCACATCAA 
GGTGCTAATA GAACCTCCTC TCTAGAAAAT CATGACCAAA TGTCTGTGAA CTGCAGAACG 
TTGCTCAGCG AGTCCCCTGG TAAGCTCCAC GTTGAAGTTT CAGCAGATCT TACAGCAATT 
AATGGTCTTT CTTACAATCA AAATCTCGCC AATGGCAGTT ATGATTTAGC TAACAGAGAC 
TTTTGCCAAG GAAATCAGCT AAATTACTAC AAGCAGGAAA TACCCGTGCA ACCAACTACC 
TCATATTCCA TTCAGAATTT ATACAGTTAC GAGAACCAGC CCCAGCCCAG CGATGAATGT 
ACTCTCCTGA GTAATAAATA CCTTGATGGA AATGCTAACA AATCTACCTC AGATGGAAGT 
CCTGTGATGG CCGTAATGCC TGGTACGACA GACACTATTC AAGTGCTCAA GGGCAGGATG 
GATTCTGAGC AGAGCCCTTC TATTGGGTAC TCCTCAAGGA CTCTTGGCCC CAATCCTGGA 
CTTATTCTTC AGGCTTTGAC TCTGTCAAAC GCTAGTGATG GATTTAACCT GGAGCGGCTT 
GAAATGCTTG GCGACTCCTT TTTAAAGCAT GCCATCACCA CATATCTATT TTGCACTTAC 
CCTGATGCGC ATGAGGGCCG CCTTTCATAT ATGAGAAGCA AAAAGGTCAG CAACTGTAAT 
CTGTATCGCC TTGGAAAAAA GAAGGGACTA CCCAGCCGCA TGGTGGTGTC AATATTTGAT 
CCCCCTGTGA ATTGGCTTCC TCCTGGTTAT GTAGTAAATC AAGACAAAAG CAACACAGAT 
AAATGGGAAA AAGATGAAAT GACAAAAGAC TGCATGCTGG CGAATGGCAA ACTGGATGAG 
GATTACGAGG AGGAGGATGA GGAGGAGGAG AGCCTGATGT GGAGGGCTCC GAAGGAAGAG 
GCTGACTATG AAGATGATTT CCTGGAGTAT GATCAGGAAC ATATCAGATT TATAGATAAT 
ATGTTAATGG GGTCAGGAGC TTTTGTAAAG AAAATCTCTC TTTCTCCTTT TTCAACCACT 
GATTCTGCAT ATGAATGGAA AATGCCCAAA AAATCCTCCT TAGGTAGTAT GCCATTTTCA 
TCAGATTTTG AGGATTTTGA CTACAGCTCT TGGGATGCAA TGTGCTATCT GGATCCTAGC 
AAAGCTGTTG AAGAAGATGA CTTTGTGGTG GGGTTCTGGA ATCCATCAGA AGAAAACTGT 
GGTGTTGACA CGGGAAAGCA GTCCATTTCT TACGACTTGC ACACTGAGCA GTGTATTGCT 
GACAAAAGCA TAGCGGACTG TGTGGAAGCC CTGCTGGGCT GCTATTTAAC CAGCTGTGGG 
GAGAGGGCTG CTCAGCTTTT CCTCTGTTCA CTGGGGCTGA AGGTGCTCCC GGTAATTAAA 
AGGACTGATC GGGAAAAGGC CCTGTGCCCT ACTCGGGAGA ATTTCAACAG CCAACAAAAG 
AACCTTTCAG TGAGCTGTGC TGCTGCTTCT GTGGCCAGTT CACGCTCTTC TGTATTGAAA 
GACTCGGAAT ATGGTTGTTT GAAGATTCCA CCAAGATGTA TGTTTGATCA TCCAGATGCA 
GATAAAACAC TGAATCACCT TATATCGGGG TTTGAAAATT TTGAAAAGAA AATCAACTAC 
AGATTCAAGA ATAAGGCTTA CCTTCTCCAG GCTTTTACAC ATGCCTCCTA CCACTACAAT 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCGCGG CAGCACTCCC CGGGGGTCCT GACAGACCTG 
CGGTCTGCCC TGGTCAACAA CACCATCTTT GCATCGCTGG CTGTAAAGTA CGACTACCAC 
AAGTACTTCA AAGCTGTCTC TCCTGAGCTC TTCCATGTCA TTGATGACTT TGTGCAGTTT 
CAGCTTGAGA AGAATGAAAT GCAAGGAATG GATTCTGAGC TTAGGAGATC TGAGGAGGAT 
GAAGAGAAAG AAGAGGATAT TGAAGTTCCA AAGGCCATGG GGGATATTTT TGAGTCGCTT 
GCTGGTGCCA TTTACATGGA TAGTGGGATG TCACTGGAGA CAGTCTGGCA GGTGTACTAT 
CCCATGATGC GGCCACTAAT AGAAAAGTTT TCTGCAAATG TACCCCGTTC CCCTGTGCGA 
GAATTGCTTG AAATGGAACC AGAAACTGCC AAATTTAGCC CGGCTGAGAG AACTTACGAC 
GGGAAGGTCA GAGTCACTGT GGAAGTAGTA GGAAAGGGGA AATTTAAAGG TGTTGGTCGA 
AGTTACAGGA TTGCCAAATC TGCAGCAGCA AGAAGAGCCC TCCGAAGCCT CAAAGCTAAT 
CAACCTCAGG TTCCCAATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534906.1
CDS	526..5889
Translation	MEKGRCSWSTLVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_011536604.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536604.2

ORF Insert Sequence:

ATGGAAAAAG GACGGTGTTC TTGGTCAACT CTGGTTCTCA TTATGACTTG CTATGTCGCC 
TTGAATGTTT TGAAAAATGG TTACTTATCA CTGTCAGACA TTAACCTTTT GGTGTTTGAT 
GAGTGTCATC TTGCAATCCT AGACCACCCC TATCGAGAAA TTATGAAGCT CTGTGAAAAT 
TGTCCATCAT GTCCTCGCAT TTTGGGACTA ACTGCTTCCA TTTTAAATGG GAAATGTGAT 
CCAGAGGAAT TGGAAGAAAA GATTCAGAAA CTAGAGAAAA TTCTTAAGAG TAATGCTGAA 
ACTGCAACTG ACCTGGTGGT CTTAGACAGG TATACTTCTC AGCCATGTGA GATTGTGGTG 
GATTGTGGAC CATTTACTGA CAGAAGTGGG CTTTATGAAA GACTGCTGAT GGAATTAGAA 
GAAGCACTTA ATTTTATCAA TGATTGTAAT ATATCTGTAC ATTCAAAAGA AAGAGATTCT 
ACTTTAATTT CGAAACAGAT ACTATCAGAC TGTCGTGCCG TATTGGTAGT TCTGGGACCC 
TGGTGTGCAG ATAAAGTAGC TGGAATGATG GTAAGAGAAC TACAGAAATA CATCAAACAT 
GAGCAAGAGG AGCTGCACAG GAAATTTTTA TTGTTTACAG ACACTTTCCT AAGGAAAATA 
CATGCACTAT GTGAAGAGCA CTTCTCACCT GCCTCACTTG ACCTGAAATT TGTAACTCCT 
AAAGTAATCA AACTGCTCGA AATCTTACGC AAATATAAAC CATATGAGCG ACAGCAGTTT 
GAAAGCGTTG AGTGGTATAA TAATAGAAAT CAGGATAATT ATGTGTCATG GAGTGATTCT 
GAGGATGATG ATGAGGATGA AGAAATTGAA GAAAAAGAGA AGCCAGAGAC AAATTTTCCT 
TCTCCTTTTA CCAACATTTT GTGCGGAATT ATTTTTGTGG AAAGAAGATA CACAGCAGTT 
GTCTTAAACA GATTGATAAA GGAAGCTGGC AAACAAGATC CAGAGCTGGC TTATATCAGT 
AGCAATTTCA TAACTGGACA TGGCATTGGG AAGAATCAGC CTCGCAACAA ACAGATGGAA 
GCAGAATTCA GAAAACAGGA AGAGGTACTT AGGAAATTTC GAGCACATGA GACCAACCTG 
CTTATTGCAA CAAGTATTGT AGAAGAGGGT GTTGATATAC CAAAATGCAA CTTGGTGGTT 
CGTTTTGATT TGCCCACAGA ATATCGATCC TATGTTCAAT CTAAAGGAAG AGCAAGGGCA 
CCCATCTCTA ATTATATAAT GTTAGCGGAT ACAGACAAAA TAAAAAGTTT TGAAGAAGAC 
CTTAAAACCT ACAAAGCTAT TGAAAAGATC TTGAGAAACA AGTGTTCCAA GTCGGTTGAT 
ACTGGTGAGA CTGACATTGA TCCTGTCATG GATGATGATG ACGTTTTCCC ACCATATGTG 
TTGAGGCCTG ACGATGGTGG TCCACGAGTC ACAATCAACA CGGCCATTGG ACACATCAAT 
AGATACTGTG CTAGATTACC AAGTGATCCG TTTACTCATC TAGCTCCTAA ATGCAGAACC 
CGAGAGTTGC CTGATGGTAC ATTTTATTCA ACTCTTTATC TGCCAATTAA CTCACCTCTT 
CGAGCCTCCA TTGTTGGTCC ACCAATGAGC TGTGTACGAT TGGCTGAAAG AGTTGTAGCT 
CTCATTTGCT GTGAGAAACT GCACAAAATT GGCGAACTGG ATGACCATTT GATGCCAGTT 
GGGAAAGAGA CTGTTAAATA TGAAGAGGAG CTTGATTTGC ATGATGAAGA AGAGACCAGT 
GTTCCAGGAA GACCAGGTTC CACGAAACGA AGGCAGTGCT ACCCAAAAGC AATTCCAGAG 
TGTTTGAGGG ATAGTTATCC CAGACCTGAT CAGCCCTGTT ACCTGTATGT GATAGGAATG 
GTTTTAACTA CACCTTTACC TGATGAACTC AACTTTAGAA GGCGGAAGCT CTATCCTCCT 
GAAGATACCA CAAGATGCTT TGGAATACTG ACGGCCAAAC CCATACCTCA GATTCCACAC 
TTTCCTGTGT ACACACGCTC TGGAGAGGTT ACCATATCCA TTGAGTTGAA GAAGTCTGGT 
TTCATGTTGT CTCTACAAAT GCTTGAGTTG ATTACAAGAC TTCACCAGTA TATATTCTCA 
CATATTCTTC GGCTTGAAAA ACCTGCACTA GAATTTAAAC CTACAGACGC TGATTCAGCA 
TACTGTGTTC TACCTCTTAA TGTTGTTAAT GACTCCAGCA CTTTGGATAT TGACTTTAAA 
TTCATGGAAG ATATTGAGAA GTCTGAAGCT CGCATAGGCA TTCCCAGTAC AAAGTATACA 
AAAGAAACAC CCTTTGTTTT TAAATTAGAA GATTACCAAG ATGCCGTTAT CATTCCAAGA 
TATCGCAATT TTGATCAGCC TCATCGATTT TATGTAGCTG ATGTGTACAC TGATCTTACC 
CCACTCAGTA AATTTCCTTC CCCTGAGTAT GAAACTTTTG CAGAATATTA TAAAACAAAG 
TACAACCTTG ACCTAACCAA TCTCAACCAG CCACTGCTGG ATGTGGACCA CACATCTTCA 
AGACTTAATC TTTTGACACC TCGACATTTG AATCAGAAGG GGAAAGCGCT TCCTTTAAGC 
AGTGCTGAGA AGAGGAAAGC CAAATGGGAA AGTCTGCAGA ATAAACAGAT ACTGGTTCCA 
GAACTCTGTG CTATACATCC AATTCCAGCA TCACTGTGGA GAAAAGCTGT TTGTCTCCCC 
AGCATACTTT ATCGCCTTCA CTGCCTTTTG ACTGCAGAGG AGCTAAGAGC CCAGACTGCC 
AGCGATGCTG GCGTGGGAGT CAGATCACTT CCTGCGGATT TTAGATACCC TAACTTAGAC 
TTCGGGTGGA AAAAATCTAT TGACAGCAAA TCTTTCATCT CAATTTCTAA CTCCTCTTCA 
GCTGAAAATG ATAATTACTG TAAGCACAGC ACAATTGTCC CTGAAAATGC TGCACATCAA 
GGTGCTAATA GAACCTCCTC TCTAGAAAAT CATGACCAAA TGTCTGTGAA CTGCAGAACG 
TTGCTCAGCG AGTCCCCTGG TAAGCTCCAC GTTGAAGTTT CAGCAGATCT TACAGCAATT 
AATGGTCTTT CTTACAATCA AAATCTCGCC AATGGCAGTT ATGATTTAGC TAACAGAGAC 
TTTTGCCAAG GAAATCAGCT AAATTACTAC AAGCAGGAAA TACCCGTGCA ACCAACTACC 
TCATATTCCA TTCAGAATTT ATACAGTTAC GAGAACCAGC CCCAGCCCAG CGATGAATGT 
ACTCTCCTGA GTAATAAATA CCTTGATGGA AATGCTAACA AATCTACCTC AGATGGAAGT 
CCTGTGATGG CCGTAATGCC TGGTACGACA GACACTATTC AAGTGCTCAA GGGCAGGATG 
GATTCTGAGC AGAGCCCTTC TATTGGGTAC TCCTCAAGGA CTCTTGGCCC CAATCCTGGA 
CTTATTCTTC AGGCTTTGAC TCTGTCAAAC GCTAGTGATG GATTTAACCT GGAGCGGCTT 
GAAATGCTTG GCGACTCCTT TTTAAAGCAT GCCATCACCA CATATCTATT TTGCACTTAC 
CCTGATGCGC ATGAGGGCCG CCTTTCATAT ATGAGAAGCA AAAAGGTCAG CAACTGTAAT 
CTGTATCGCC TTGGAAAAAA GAAGGGACTA CCCAGCCGCA TGGTGGTGTC AATATTTGAT 
CCCCCTGTGA ATTGGCTTCC TCCTGGTTAT GTAGTAAATC AAGACAAAAG CAACACAGAT 
AAATGGGAAA AAGATGAAAT GACAAAAGAC TGCATGCTGG CGAATGGCAA ACTGGATGAG 
GATTACGAGG AGGAGGATGA GGAGGAGGAG AGCCTGATGT GGAGGGCTCC GAAGGAAGAG 
GCTGACTATG AAGATGATTT CCTGGAGTAT GATCAGGAAC ATATCAGATT TATAGATAAT 
ATGTTAATGG GGTCAGGAGC TTTTGTAAAG AAAATCTCTC TTTCTCCTTT TTCAACCACT 
GATTCTGCAT ATGAATGGAA AATGCCCAAA AAATCCTCCT TAGGTAGTAT GCCATTTTCA 
TCAGATTTTG AGGATTTTGA CTACAGCTCT TGGGATGCAA TGTGCTATCT GGATCCTAGC 
AAAGCTGTTG AAGAAGATGA CTTTGTGGTG GGGTTCTGGA ATCCATCAGA AGAAAACTGT 
GGTGTTGACA CGGGAAAGCA GTCCATTTCT TACGACTTGC ACACTGAGCA GTGTATTGCT 
GACAAAAGCA TAGCGGACTG TGTGGAAGCC CTGCTGGGCT GCTATTTAAC CAGCTGTGGG 
GAGAGGGCTG CTCAGCTTTT CCTCTGTTCA CTGGGGCTGA AGGTGCTCCC GGTAATTAAA 
AGGACTGATC GGGAAAAGGC CCTGTGCCCT ACTCGGGAGA ATTTCAACAG CCAACAAAAG 
AACCTTTCAG TGAGCTGTGC TGCTGCTTCT GTGGCCAGTT CACGCTCTTC TGTATTGAAA 
GACTCGGAAT ATGGTTGTTT GAAGATTCCA CCAAGATGTA TGTTTGATCA TCCAGATGCA 
GATAAAACAC TGAATCACCT TATATCGGGG TTTGAAAATT TTGAAAAGAA AATCAACTAC 
AGATTCAAGA ATAAGGCTTA CCTTCTCCAG GCTTTTACAC ATGCCTCCTA CCACTACAAT 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCGCGG CAGCACTCCC CGGGGGTCCT GACAGACCTG 
CGGTCTGCCC TGGTCAACAA CACCATCTTT GCATCGCTGG CTGTAAAGTA CGACTACCAC 
AAGTACTTCA AAGCTGTCTC TCCTGAGCTC TTCCATGTCA TTGATGACTT TGTGCAGTTT 
CAGCTTGAGA AGAATGAAAT GCAAGGAATG GATTCTGAGC TTAGGAGATC TGAGGAGGAT 
GAAGAGAAAG AAGAGGATAT TGAAGTTCCA AAGGCCATGG GGGATATTTT TGAGTCGCTT 
GCTGGTGCCA TTTACATGGA TAGTGGGATG TCACTGGAGA CAGTCTGGCA GGTGTACTAT 
CCCATGATGC GGCCACTAAT AGAAAAGTTT TCTGCAAATG TACCCCGTTC CCCTGTGCGA 
GAATTGCTTG AAATGGAACC AGAAACTGCC AAATTTAGCC CGGCTGAGAG AACTTACGAC 
GGGAAGGTCA GAGTCACTGT GGAAGTAGTA GGAAAGGGGA AATTTAAAGG TGTTGGTCGA 
AGTTACAGGA TTGCCAAATC TGCAGCAGCA AGAAGAGCCC TCCGAAGCCT CAAAGCTAAT 
CAACCTCAGG TTCCCAATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_017021123.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876612.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X8, mRNA.

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CloneID	OHu66150
Clone ID Related Accession (Same CDS sequence)	XM_011536604.2 , XM_017021123.1 , XM_017021123.2 , XM_017021122.1 , XM_017021122.2
Accession Version	XM_017021123.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAG GACGGTGTTC TTGGTCAACT CTGGTTCTCA TTATGACTTG CTATGTCGCC 
TTGAATGTTT TGAAAAATGG TTACTTATCA CTGTCAGACA TTAACCTTTT GGTGTTTGAT 
GAGTGTCATC TTGCAATCCT AGACCACCCC TATCGAGAAA TTATGAAGCT CTGTGAAAAT 
TGTCCATCAT GTCCTCGCAT TTTGGGACTA ACTGCTTCCA TTTTAAATGG GAAATGTGAT 
CCAGAGGAAT TGGAAGAAAA GATTCAGAAA CTAGAGAAAA TTCTTAAGAG TAATGCTGAA 
ACTGCAACTG ACCTGGTGGT CTTAGACAGG TATACTTCTC AGCCATGTGA GATTGTGGTG 
GATTGTGGAC CATTTACTGA CAGAAGTGGG CTTTATGAAA GACTGCTGAT GGAATTAGAA 
GAAGCACTTA ATTTTATCAA TGATTGTAAT ATATCTGTAC ATTCAAAAGA AAGAGATTCT 
ACTTTAATTT CGAAACAGAT ACTATCAGAC TGTCGTGCCG TATTGGTAGT TCTGGGACCC 
TGGTGTGCAG ATAAAGTAGC TGGAATGATG GTAAGAGAAC TACAGAAATA CATCAAACAT 
GAGCAAGAGG AGCTGCACAG GAAATTTTTA TTGTTTACAG ACACTTTCCT AAGGAAAATA 
CATGCACTAT GTGAAGAGCA CTTCTCACCT GCCTCACTTG ACCTGAAATT TGTAACTCCT 
AAAGTAATCA AACTGCTCGA AATCTTACGC AAATATAAAC CATATGAGCG ACAGCAGTTT 
GAAAGCGTTG AGTGGTATAA TAATAGAAAT CAGGATAATT ATGTGTCATG GAGTGATTCT 
GAGGATGATG ATGAGGATGA AGAAATTGAA GAAAAAGAGA AGCCAGAGAC AAATTTTCCT 
TCTCCTTTTA CCAACATTTT GTGCGGAATT ATTTTTGTGG AAAGAAGATA CACAGCAGTT 
GTCTTAAACA GATTGATAAA GGAAGCTGGC AAACAAGATC CAGAGCTGGC TTATATCAGT 
AGCAATTTCA TAACTGGACA TGGCATTGGG AAGAATCAGC CTCGCAACAA ACAGATGGAA 
GCAGAATTCA GAAAACAGGA AGAGGTACTT AGGAAATTTC GAGCACATGA GACCAACCTG 
CTTATTGCAA CAAGTATTGT AGAAGAGGGT GTTGATATAC CAAAATGCAA CTTGGTGGTT 
CGTTTTGATT TGCCCACAGA ATATCGATCC TATGTTCAAT CTAAAGGAAG AGCAAGGGCA 
CCCATCTCTA ATTATATAAT GTTAGCGGAT ACAGACAAAA TAAAAAGTTT TGAAGAAGAC 
CTTAAAACCT ACAAAGCTAT TGAAAAGATC TTGAGAAACA AGTGTTCCAA GTCGGTTGAT 
ACTGGTGAGA CTGACATTGA TCCTGTCATG GATGATGATG ACGTTTTCCC ACCATATGTG 
TTGAGGCCTG ACGATGGTGG TCCACGAGTC ACAATCAACA CGGCCATTGG ACACATCAAT 
AGATACTGTG CTAGATTACC AAGTGATCCG TTTACTCATC TAGCTCCTAA ATGCAGAACC 
CGAGAGTTGC CTGATGGTAC ATTTTATTCA ACTCTTTATC TGCCAATTAA CTCACCTCTT 
CGAGCCTCCA TTGTTGGTCC ACCAATGAGC TGTGTACGAT TGGCTGAAAG AGTTGTAGCT 
CTCATTTGCT GTGAGAAACT GCACAAAATT GGCGAACTGG ATGACCATTT GATGCCAGTT 
GGGAAAGAGA CTGTTAAATA TGAAGAGGAG CTTGATTTGC ATGATGAAGA AGAGACCAGT 
GTTCCAGGAA GACCAGGTTC CACGAAACGA AGGCAGTGCT ACCCAAAAGC AATTCCAGAG 
TGTTTGAGGG ATAGTTATCC CAGACCTGAT CAGCCCTGTT ACCTGTATGT GATAGGAATG 
GTTTTAACTA CACCTTTACC TGATGAACTC AACTTTAGAA GGCGGAAGCT CTATCCTCCT 
GAAGATACCA CAAGATGCTT TGGAATACTG ACGGCCAAAC CCATACCTCA GATTCCACAC 
TTTCCTGTGT ACACACGCTC TGGAGAGGTT ACCATATCCA TTGAGTTGAA GAAGTCTGGT 
TTCATGTTGT CTCTACAAAT GCTTGAGTTG ATTACAAGAC TTCACCAGTA TATATTCTCA 
CATATTCTTC GGCTTGAAAA ACCTGCACTA GAATTTAAAC CTACAGACGC TGATTCAGCA 
TACTGTGTTC TACCTCTTAA TGTTGTTAAT GACTCCAGCA CTTTGGATAT TGACTTTAAA 
TTCATGGAAG ATATTGAGAA GTCTGAAGCT CGCATAGGCA TTCCCAGTAC AAAGTATACA 
AAAGAAACAC CCTTTGTTTT TAAATTAGAA GATTACCAAG ATGCCGTTAT CATTCCAAGA 
TATCGCAATT TTGATCAGCC TCATCGATTT TATGTAGCTG ATGTGTACAC TGATCTTACC 
CCACTCAGTA AATTTCCTTC CCCTGAGTAT GAAACTTTTG CAGAATATTA TAAAACAAAG 
TACAACCTTG ACCTAACCAA TCTCAACCAG CCACTGCTGG ATGTGGACCA CACATCTTCA 
AGACTTAATC TTTTGACACC TCGACATTTG AATCAGAAGG GGAAAGCGCT TCCTTTAAGC 
AGTGCTGAGA AGAGGAAAGC CAAATGGGAA AGTCTGCAGA ATAAACAGAT ACTGGTTCCA 
GAACTCTGTG CTATACATCC AATTCCAGCA TCACTGTGGA GAAAAGCTGT TTGTCTCCCC 
AGCATACTTT ATCGCCTTCA CTGCCTTTTG ACTGCAGAGG AGCTAAGAGC CCAGACTGCC 
AGCGATGCTG GCGTGGGAGT CAGATCACTT CCTGCGGATT TTAGATACCC TAACTTAGAC 
TTCGGGTGGA AAAAATCTAT TGACAGCAAA TCTTTCATCT CAATTTCTAA CTCCTCTTCA 
GCTGAAAATG ATAATTACTG TAAGCACAGC ACAATTGTCC CTGAAAATGC TGCACATCAA 
GGTGCTAATA GAACCTCCTC TCTAGAAAAT CATGACCAAA TGTCTGTGAA CTGCAGAACG 
TTGCTCAGCG AGTCCCCTGG TAAGCTCCAC GTTGAAGTTT CAGCAGATCT TACAGCAATT 
AATGGTCTTT CTTACAATCA AAATCTCGCC AATGGCAGTT ATGATTTAGC TAACAGAGAC 
TTTTGCCAAG GAAATCAGCT AAATTACTAC AAGCAGGAAA TACCCGTGCA ACCAACTACC 
TCATATTCCA TTCAGAATTT ATACAGTTAC GAGAACCAGC CCCAGCCCAG CGATGAATGT 
ACTCTCCTGA GTAATAAATA CCTTGATGGA AATGCTAACA AATCTACCTC AGATGGAAGT 
CCTGTGATGG CCGTAATGCC TGGTACGACA GACACTATTC AAGTGCTCAA GGGCAGGATG 
GATTCTGAGC AGAGCCCTTC TATTGGGTAC TCCTCAAGGA CTCTTGGCCC CAATCCTGGA 
CTTATTCTTC AGGCTTTGAC TCTGTCAAAC GCTAGTGATG GATTTAACCT GGAGCGGCTT 
GAAATGCTTG GCGACTCCTT TTTAAAGCAT GCCATCACCA CATATCTATT TTGCACTTAC 
CCTGATGCGC ATGAGGGCCG CCTTTCATAT ATGAGAAGCA AAAAGGTCAG CAACTGTAAT 
CTGTATCGCC TTGGAAAAAA GAAGGGACTA CCCAGCCGCA TGGTGGTGTC AATATTTGAT 
CCCCCTGTGA ATTGGCTTCC TCCTGGTTAT GTAGTAAATC AAGACAAAAG CAACACAGAT 
AAATGGGAAA AAGATGAAAT GACAAAAGAC TGCATGCTGG CGAATGGCAA ACTGGATGAG 
GATTACGAGG AGGAGGATGA GGAGGAGGAG AGCCTGATGT GGAGGGCTCC GAAGGAAGAG 
GCTGACTATG AAGATGATTT CCTGGAGTAT GATCAGGAAC ATATCAGATT TATAGATAAT 
ATGTTAATGG GGTCAGGAGC TTTTGTAAAG AAAATCTCTC TTTCTCCTTT TTCAACCACT 
GATTCTGCAT ATGAATGGAA AATGCCCAAA AAATCCTCCT TAGGTAGTAT GCCATTTTCA 
TCAGATTTTG AGGATTTTGA CTACAGCTCT TGGGATGCAA TGTGCTATCT GGATCCTAGC 
AAAGCTGTTG AAGAAGATGA CTTTGTGGTG GGGTTCTGGA ATCCATCAGA AGAAAACTGT 
GGTGTTGACA CGGGAAAGCA GTCCATTTCT TACGACTTGC ACACTGAGCA GTGTATTGCT 
GACAAAAGCA TAGCGGACTG TGTGGAAGCC CTGCTGGGCT GCTATTTAAC CAGCTGTGGG 
GAGAGGGCTG CTCAGCTTTT CCTCTGTTCA CTGGGGCTGA AGGTGCTCCC GGTAATTAAA 
AGGACTGATC GGGAAAAGGC CCTGTGCCCT ACTCGGGAGA ATTTCAACAG CCAACAAAAG 
AACCTTTCAG TGAGCTGTGC TGCTGCTTCT GTGGCCAGTT CACGCTCTTC TGTATTGAAA 
GACTCGGAAT ATGGTTGTTT GAAGATTCCA CCAAGATGTA TGTTTGATCA TCCAGATGCA 
GATAAAACAC TGAATCACCT TATATCGGGG TTTGAAAATT TTGAAAAGAA AATCAACTAC 
AGATTCAAGA ATAAGGCTTA CCTTCTCCAG GCTTTTACAC ATGCCTCCTA CCACTACAAT 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCGCGG CAGCACTCCC CGGGGGTCCT GACAGACCTG 
CGGTCTGCCC TGGTCAACAA CACCATCTTT GCATCGCTGG CTGTAAAGTA CGACTACCAC 
AAGTACTTCA AAGCTGTCTC TCCTGAGCTC TTCCATGTCA TTGATGACTT TGTGCAGTTT 
CAGCTTGAGA AGAATGAAAT GCAAGGAATG GATTCTGAGC TTAGGAGATC TGAGGAGGAT 
GAAGAGAAAG AAGAGGATAT TGAAGTTCCA AAGGCCATGG GGGATATTTT TGAGTCGCTT 
GCTGGTGCCA TTTACATGGA TAGTGGGATG TCACTGGAGA CAGTCTGGCA GGTGTACTAT 
CCCATGATGC GGCCACTAAT AGAAAAGTTT TCTGCAAATG TACCCCGTTC CCCTGTGCGA 
GAATTGCTTG AAATGGAACC AGAAACTGCC AAATTTAGCC CGGCTGAGAG AACTTACGAC 
GGGAAGGTCA GAGTCACTGT GGAAGTAGTA GGAAAGGGGA AATTTAAAGG TGTTGGTCGA 
AGTTACAGGA TTGCCAAATC TGCAGCAGCA AGAAGAGCCC TCCGAAGCCT CAAAGCTAAT 
CAACCTCAGG TTCCCAATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876612.1
CDS	1007..6370
Translation	MEKGRCSWSTLVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_017021123.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021123.1

ORF Insert Sequence:

ATGGAAAAAG GACGGTGTTC TTGGTCAACT CTGGTTCTCA TTATGACTTG CTATGTCGCC 
TTGAATGTTT TGAAAAATGG TTACTTATCA CTGTCAGACA TTAACCTTTT GGTGTTTGAT 
GAGTGTCATC TTGCAATCCT AGACCACCCC TATCGAGAAA TTATGAAGCT CTGTGAAAAT 
TGTCCATCAT GTCCTCGCAT TTTGGGACTA ACTGCTTCCA TTTTAAATGG GAAATGTGAT 
CCAGAGGAAT TGGAAGAAAA GATTCAGAAA CTAGAGAAAA TTCTTAAGAG TAATGCTGAA 
ACTGCAACTG ACCTGGTGGT CTTAGACAGG TATACTTCTC AGCCATGTGA GATTGTGGTG 
GATTGTGGAC CATTTACTGA CAGAAGTGGG CTTTATGAAA GACTGCTGAT GGAATTAGAA 
GAAGCACTTA ATTTTATCAA TGATTGTAAT ATATCTGTAC ATTCAAAAGA AAGAGATTCT 
ACTTTAATTT CGAAACAGAT ACTATCAGAC TGTCGTGCCG TATTGGTAGT TCTGGGACCC 
TGGTGTGCAG ATAAAGTAGC TGGAATGATG GTAAGAGAAC TACAGAAATA CATCAAACAT 
GAGCAAGAGG AGCTGCACAG GAAATTTTTA TTGTTTACAG ACACTTTCCT AAGGAAAATA 
CATGCACTAT GTGAAGAGCA CTTCTCACCT GCCTCACTTG ACCTGAAATT TGTAACTCCT 
AAAGTAATCA AACTGCTCGA AATCTTACGC AAATATAAAC CATATGAGCG ACAGCAGTTT 
GAAAGCGTTG AGTGGTATAA TAATAGAAAT CAGGATAATT ATGTGTCATG GAGTGATTCT 
GAGGATGATG ATGAGGATGA AGAAATTGAA GAAAAAGAGA AGCCAGAGAC AAATTTTCCT 
TCTCCTTTTA CCAACATTTT GTGCGGAATT ATTTTTGTGG AAAGAAGATA CACAGCAGTT 
GTCTTAAACA GATTGATAAA GGAAGCTGGC AAACAAGATC CAGAGCTGGC TTATATCAGT 
AGCAATTTCA TAACTGGACA TGGCATTGGG AAGAATCAGC CTCGCAACAA ACAGATGGAA 
GCAGAATTCA GAAAACAGGA AGAGGTACTT AGGAAATTTC GAGCACATGA GACCAACCTG 
CTTATTGCAA CAAGTATTGT AGAAGAGGGT GTTGATATAC CAAAATGCAA CTTGGTGGTT 
CGTTTTGATT TGCCCACAGA ATATCGATCC TATGTTCAAT CTAAAGGAAG AGCAAGGGCA 
CCCATCTCTA ATTATATAAT GTTAGCGGAT ACAGACAAAA TAAAAAGTTT TGAAGAAGAC 
CTTAAAACCT ACAAAGCTAT TGAAAAGATC TTGAGAAACA AGTGTTCCAA GTCGGTTGAT 
ACTGGTGAGA CTGACATTGA TCCTGTCATG GATGATGATG ACGTTTTCCC ACCATATGTG 
TTGAGGCCTG ACGATGGTGG TCCACGAGTC ACAATCAACA CGGCCATTGG ACACATCAAT 
AGATACTGTG CTAGATTACC AAGTGATCCG TTTACTCATC TAGCTCCTAA ATGCAGAACC 
CGAGAGTTGC CTGATGGTAC ATTTTATTCA ACTCTTTATC TGCCAATTAA CTCACCTCTT 
CGAGCCTCCA TTGTTGGTCC ACCAATGAGC TGTGTACGAT TGGCTGAAAG AGTTGTAGCT 
CTCATTTGCT GTGAGAAACT GCACAAAATT GGCGAACTGG ATGACCATTT GATGCCAGTT 
GGGAAAGAGA CTGTTAAATA TGAAGAGGAG CTTGATTTGC ATGATGAAGA AGAGACCAGT 
GTTCCAGGAA GACCAGGTTC CACGAAACGA AGGCAGTGCT ACCCAAAAGC AATTCCAGAG 
TGTTTGAGGG ATAGTTATCC CAGACCTGAT CAGCCCTGTT ACCTGTATGT GATAGGAATG 
GTTTTAACTA CACCTTTACC TGATGAACTC AACTTTAGAA GGCGGAAGCT CTATCCTCCT 
GAAGATACCA CAAGATGCTT TGGAATACTG ACGGCCAAAC CCATACCTCA GATTCCACAC 
TTTCCTGTGT ACACACGCTC TGGAGAGGTT ACCATATCCA TTGAGTTGAA GAAGTCTGGT 
TTCATGTTGT CTCTACAAAT GCTTGAGTTG ATTACAAGAC TTCACCAGTA TATATTCTCA 
CATATTCTTC GGCTTGAAAA ACCTGCACTA GAATTTAAAC CTACAGACGC TGATTCAGCA 
TACTGTGTTC TACCTCTTAA TGTTGTTAAT GACTCCAGCA CTTTGGATAT TGACTTTAAA 
TTCATGGAAG ATATTGAGAA GTCTGAAGCT CGCATAGGCA TTCCCAGTAC AAAGTATACA 
AAAGAAACAC CCTTTGTTTT TAAATTAGAA GATTACCAAG ATGCCGTTAT CATTCCAAGA 
TATCGCAATT TTGATCAGCC TCATCGATTT TATGTAGCTG ATGTGTACAC TGATCTTACC 
CCACTCAGTA AATTTCCTTC CCCTGAGTAT GAAACTTTTG CAGAATATTA TAAAACAAAG 
TACAACCTTG ACCTAACCAA TCTCAACCAG CCACTGCTGG ATGTGGACCA CACATCTTCA 
AGACTTAATC TTTTGACACC TCGACATTTG AATCAGAAGG GGAAAGCGCT TCCTTTAAGC 
AGTGCTGAGA AGAGGAAAGC CAAATGGGAA AGTCTGCAGA ATAAACAGAT ACTGGTTCCA 
GAACTCTGTG CTATACATCC AATTCCAGCA TCACTGTGGA GAAAAGCTGT TTGTCTCCCC 
AGCATACTTT ATCGCCTTCA CTGCCTTTTG ACTGCAGAGG AGCTAAGAGC CCAGACTGCC 
AGCGATGCTG GCGTGGGAGT CAGATCACTT CCTGCGGATT TTAGATACCC TAACTTAGAC 
TTCGGGTGGA AAAAATCTAT TGACAGCAAA TCTTTCATCT CAATTTCTAA CTCCTCTTCA 
GCTGAAAATG ATAATTACTG TAAGCACAGC ACAATTGTCC CTGAAAATGC TGCACATCAA 
GGTGCTAATA GAACCTCCTC TCTAGAAAAT CATGACCAAA TGTCTGTGAA CTGCAGAACG 
TTGCTCAGCG AGTCCCCTGG TAAGCTCCAC GTTGAAGTTT CAGCAGATCT TACAGCAATT 
AATGGTCTTT CTTACAATCA AAATCTCGCC AATGGCAGTT ATGATTTAGC TAACAGAGAC 
TTTTGCCAAG GAAATCAGCT AAATTACTAC AAGCAGGAAA TACCCGTGCA ACCAACTACC 
TCATATTCCA TTCAGAATTT ATACAGTTAC GAGAACCAGC CCCAGCCCAG CGATGAATGT 
ACTCTCCTGA GTAATAAATA CCTTGATGGA AATGCTAACA AATCTACCTC AGATGGAAGT 
CCTGTGATGG CCGTAATGCC TGGTACGACA GACACTATTC AAGTGCTCAA GGGCAGGATG 
GATTCTGAGC AGAGCCCTTC TATTGGGTAC TCCTCAAGGA CTCTTGGCCC CAATCCTGGA 
CTTATTCTTC AGGCTTTGAC TCTGTCAAAC GCTAGTGATG GATTTAACCT GGAGCGGCTT 
GAAATGCTTG GCGACTCCTT TTTAAAGCAT GCCATCACCA CATATCTATT TTGCACTTAC 
CCTGATGCGC ATGAGGGCCG CCTTTCATAT ATGAGAAGCA AAAAGGTCAG CAACTGTAAT 
CTGTATCGCC TTGGAAAAAA GAAGGGACTA CCCAGCCGCA TGGTGGTGTC AATATTTGAT 
CCCCCTGTGA ATTGGCTTCC TCCTGGTTAT GTAGTAAATC AAGACAAAAG CAACACAGAT 
AAATGGGAAA AAGATGAAAT GACAAAAGAC TGCATGCTGG CGAATGGCAA ACTGGATGAG 
GATTACGAGG AGGAGGATGA GGAGGAGGAG AGCCTGATGT GGAGGGCTCC GAAGGAAGAG 
GCTGACTATG AAGATGATTT CCTGGAGTAT GATCAGGAAC ATATCAGATT TATAGATAAT 
ATGTTAATGG GGTCAGGAGC TTTTGTAAAG AAAATCTCTC TTTCTCCTTT TTCAACCACT 
GATTCTGCAT ATGAATGGAA AATGCCCAAA AAATCCTCCT TAGGTAGTAT GCCATTTTCA 
TCAGATTTTG AGGATTTTGA CTACAGCTCT TGGGATGCAA TGTGCTATCT GGATCCTAGC 
AAAGCTGTTG AAGAAGATGA CTTTGTGGTG GGGTTCTGGA ATCCATCAGA AGAAAACTGT 
GGTGTTGACA CGGGAAAGCA GTCCATTTCT TACGACTTGC ACACTGAGCA GTGTATTGCT 
GACAAAAGCA TAGCGGACTG TGTGGAAGCC CTGCTGGGCT GCTATTTAAC CAGCTGTGGG 
GAGAGGGCTG CTCAGCTTTT CCTCTGTTCA CTGGGGCTGA AGGTGCTCCC GGTAATTAAA 
AGGACTGATC GGGAAAAGGC CCTGTGCCCT ACTCGGGAGA ATTTCAACAG CCAACAAAAG 
AACCTTTCAG TGAGCTGTGC TGCTGCTTCT GTGGCCAGTT CACGCTCTTC TGTATTGAAA 
GACTCGGAAT ATGGTTGTTT GAAGATTCCA CCAAGATGTA TGTTTGATCA TCCAGATGCA 
GATAAAACAC TGAATCACCT TATATCGGGG TTTGAAAATT TTGAAAAGAA AATCAACTAC 
AGATTCAAGA ATAAGGCTTA CCTTCTCCAG GCTTTTACAC ATGCCTCCTA CCACTACAAT 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCGCGG CAGCACTCCC CGGGGGTCCT GACAGACCTG 
CGGTCTGCCC TGGTCAACAA CACCATCTTT GCATCGCTGG CTGTAAAGTA CGACTACCAC 
AAGTACTTCA AAGCTGTCTC TCCTGAGCTC TTCCATGTCA TTGATGACTT TGTGCAGTTT 
CAGCTTGAGA AGAATGAAAT GCAAGGAATG GATTCTGAGC TTAGGAGATC TGAGGAGGAT 
GAAGAGAAAG AAGAGGATAT TGAAGTTCCA AAGGCCATGG GGGATATTTT TGAGTCGCTT 
GCTGGTGCCA TTTACATGGA TAGTGGGATG TCACTGGAGA CAGTCTGGCA GGTGTACTAT 
CCCATGATGC GGCCACTAAT AGAAAAGTTT TCTGCAAATG TACCCCGTTC CCCTGTGCGA 
GAATTGCTTG AAATGGAACC AGAAACTGCC AAATTTAGCC CGGCTGAGAG AACTTACGAC 
GGGAAGGTCA GAGTCACTGT GGAAGTAGTA GGAAAGGGGA AATTTAAAGG TGTTGGTCGA 
AGTTACAGGA TTGCCAAATC TGCAGCAGCA AGAAGAGCCC TCCGAAGCCT CAAAGCTAAT 
CAACCTCAGG TTCCCAATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_017021123.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876612.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X8, mRNA.

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CloneID	OHu66150
Clone ID Related Accession (Same CDS sequence)	XM_011536604.2 , XM_017021123.1 , XM_017021123.2 , XM_017021122.1 , XM_017021122.2
Accession Version	XM_017021123.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021123.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAG GACGGTGTTC TTGGTCAACT CTGGTTCTCA TTATGACTTG CTATGTCGCC 
TTGAATGTTT TGAAAAATGG TTACTTATCA CTGTCAGACA TTAACCTTTT GGTGTTTGAT 
GAGTGTCATC TTGCAATCCT AGACCACCCC TATCGAGAAA TTATGAAGCT CTGTGAAAAT 
TGTCCATCAT GTCCTCGCAT TTTGGGACTA ACTGCTTCCA TTTTAAATGG GAAATGTGAT 
CCAGAGGAAT TGGAAGAAAA GATTCAGAAA CTAGAGAAAA TTCTTAAGAG TAATGCTGAA 
ACTGCAACTG ACCTGGTGGT CTTAGACAGG TATACTTCTC AGCCATGTGA GATTGTGGTG 
GATTGTGGAC CATTTACTGA CAGAAGTGGG CTTTATGAAA GACTGCTGAT GGAATTAGAA 
GAAGCACTTA ATTTTATCAA TGATTGTAAT ATATCTGTAC ATTCAAAAGA AAGAGATTCT 
ACTTTAATTT CGAAACAGAT ACTATCAGAC TGTCGTGCCG TATTGGTAGT TCTGGGACCC 
TGGTGTGCAG ATAAAGTAGC TGGAATGATG GTAAGAGAAC TACAGAAATA CATCAAACAT 
GAGCAAGAGG AGCTGCACAG GAAATTTTTA TTGTTTACAG ACACTTTCCT AAGGAAAATA 
CATGCACTAT GTGAAGAGCA CTTCTCACCT GCCTCACTTG ACCTGAAATT TGTAACTCCT 
AAAGTAATCA AACTGCTCGA AATCTTACGC AAATATAAAC CATATGAGCG ACAGCAGTTT 
GAAAGCGTTG AGTGGTATAA TAATAGAAAT CAGGATAATT ATGTGTCATG GAGTGATTCT 
GAGGATGATG ATGAGGATGA AGAAATTGAA GAAAAAGAGA AGCCAGAGAC AAATTTTCCT 
TCTCCTTTTA CCAACATTTT GTGCGGAATT ATTTTTGTGG AAAGAAGATA CACAGCAGTT 
GTCTTAAACA GATTGATAAA GGAAGCTGGC AAACAAGATC CAGAGCTGGC TTATATCAGT 
AGCAATTTCA TAACTGGACA TGGCATTGGG AAGAATCAGC CTCGCAACAA ACAGATGGAA 
GCAGAATTCA GAAAACAGGA AGAGGTACTT AGGAAATTTC GAGCACATGA GACCAACCTG 
CTTATTGCAA CAAGTATTGT AGAAGAGGGT GTTGATATAC CAAAATGCAA CTTGGTGGTT 
CGTTTTGATT TGCCCACAGA ATATCGATCC TATGTTCAAT CTAAAGGAAG AGCAAGGGCA 
CCCATCTCTA ATTATATAAT GTTAGCGGAT ACAGACAAAA TAAAAAGTTT TGAAGAAGAC 
CTTAAAACCT ACAAAGCTAT TGAAAAGATC TTGAGAAACA AGTGTTCCAA GTCGGTTGAT 
ACTGGTGAGA CTGACATTGA TCCTGTCATG GATGATGATG ACGTTTTCCC ACCATATGTG 
TTGAGGCCTG ACGATGGTGG TCCACGAGTC ACAATCAACA CGGCCATTGG ACACATCAAT 
AGATACTGTG CTAGATTACC AAGTGATCCG TTTACTCATC TAGCTCCTAA ATGCAGAACC 
CGAGAGTTGC CTGATGGTAC ATTTTATTCA ACTCTTTATC TGCCAATTAA CTCACCTCTT 
CGAGCCTCCA TTGTTGGTCC ACCAATGAGC TGTGTACGAT TGGCTGAAAG AGTTGTAGCT 
CTCATTTGCT GTGAGAAACT GCACAAAATT GGCGAACTGG ATGACCATTT GATGCCAGTT 
GGGAAAGAGA CTGTTAAATA TGAAGAGGAG CTTGATTTGC ATGATGAAGA AGAGACCAGT 
GTTCCAGGAA GACCAGGTTC CACGAAACGA AGGCAGTGCT ACCCAAAAGC AATTCCAGAG 
TGTTTGAGGG ATAGTTATCC CAGACCTGAT CAGCCCTGTT ACCTGTATGT GATAGGAATG 
GTTTTAACTA CACCTTTACC TGATGAACTC AACTTTAGAA GGCGGAAGCT CTATCCTCCT 
GAAGATACCA CAAGATGCTT TGGAATACTG ACGGCCAAAC CCATACCTCA GATTCCACAC 
TTTCCTGTGT ACACACGCTC TGGAGAGGTT ACCATATCCA TTGAGTTGAA GAAGTCTGGT 
TTCATGTTGT CTCTACAAAT GCTTGAGTTG ATTACAAGAC TTCACCAGTA TATATTCTCA 
CATATTCTTC GGCTTGAAAA ACCTGCACTA GAATTTAAAC CTACAGACGC TGATTCAGCA 
TACTGTGTTC TACCTCTTAA TGTTGTTAAT GACTCCAGCA CTTTGGATAT TGACTTTAAA 
TTCATGGAAG ATATTGAGAA GTCTGAAGCT CGCATAGGCA TTCCCAGTAC AAAGTATACA 
AAAGAAACAC CCTTTGTTTT TAAATTAGAA GATTACCAAG ATGCCGTTAT CATTCCAAGA 
TATCGCAATT TTGATCAGCC TCATCGATTT TATGTAGCTG ATGTGTACAC TGATCTTACC 
CCACTCAGTA AATTTCCTTC CCCTGAGTAT GAAACTTTTG CAGAATATTA TAAAACAAAG 
TACAACCTTG ACCTAACCAA TCTCAACCAG CCACTGCTGG ATGTGGACCA CACATCTTCA 
AGACTTAATC TTTTGACACC TCGACATTTG AATCAGAAGG GGAAAGCGCT TCCTTTAAGC 
AGTGCTGAGA AGAGGAAAGC CAAATGGGAA AGTCTGCAGA ATAAACAGAT ACTGGTTCCA 
GAACTCTGTG CTATACATCC AATTCCAGCA TCACTGTGGA GAAAAGCTGT TTGTCTCCCC 
AGCATACTTT ATCGCCTTCA CTGCCTTTTG ACTGCAGAGG AGCTAAGAGC CCAGACTGCC 
AGCGATGCTG GCGTGGGAGT CAGATCACTT CCTGCGGATT TTAGATACCC TAACTTAGAC 
TTCGGGTGGA AAAAATCTAT TGACAGCAAA TCTTTCATCT CAATTTCTAA CTCCTCTTCA 
GCTGAAAATG ATAATTACTG TAAGCACAGC ACAATTGTCC CTGAAAATGC TGCACATCAA 
GGTGCTAATA GAACCTCCTC TCTAGAAAAT CATGACCAAA TGTCTGTGAA CTGCAGAACG 
TTGCTCAGCG AGTCCCCTGG TAAGCTCCAC GTTGAAGTTT CAGCAGATCT TACAGCAATT 
AATGGTCTTT CTTACAATCA AAATCTCGCC AATGGCAGTT ATGATTTAGC TAACAGAGAC 
TTTTGCCAAG GAAATCAGCT AAATTACTAC AAGCAGGAAA TACCCGTGCA ACCAACTACC 
TCATATTCCA TTCAGAATTT ATACAGTTAC GAGAACCAGC CCCAGCCCAG CGATGAATGT 
ACTCTCCTGA GTAATAAATA CCTTGATGGA AATGCTAACA AATCTACCTC AGATGGAAGT 
CCTGTGATGG CCGTAATGCC TGGTACGACA GACACTATTC AAGTGCTCAA GGGCAGGATG 
GATTCTGAGC AGAGCCCTTC TATTGGGTAC TCCTCAAGGA CTCTTGGCCC CAATCCTGGA 
CTTATTCTTC AGGCTTTGAC TCTGTCAAAC GCTAGTGATG GATTTAACCT GGAGCGGCTT 
GAAATGCTTG GCGACTCCTT TTTAAAGCAT GCCATCACCA CATATCTATT TTGCACTTAC 
CCTGATGCGC ATGAGGGCCG CCTTTCATAT ATGAGAAGCA AAAAGGTCAG CAACTGTAAT 
CTGTATCGCC TTGGAAAAAA GAAGGGACTA CCCAGCCGCA TGGTGGTGTC AATATTTGAT 
CCCCCTGTGA ATTGGCTTCC TCCTGGTTAT GTAGTAAATC AAGACAAAAG CAACACAGAT 
AAATGGGAAA AAGATGAAAT GACAAAAGAC TGCATGCTGG CGAATGGCAA ACTGGATGAG 
GATTACGAGG AGGAGGATGA GGAGGAGGAG AGCCTGATGT GGAGGGCTCC GAAGGAAGAG 
GCTGACTATG AAGATGATTT CCTGGAGTAT GATCAGGAAC ATATCAGATT TATAGATAAT 
ATGTTAATGG GGTCAGGAGC TTTTGTAAAG AAAATCTCTC TTTCTCCTTT TTCAACCACT 
GATTCTGCAT ATGAATGGAA AATGCCCAAA AAATCCTCCT TAGGTAGTAT GCCATTTTCA 
TCAGATTTTG AGGATTTTGA CTACAGCTCT TGGGATGCAA TGTGCTATCT GGATCCTAGC 
AAAGCTGTTG AAGAAGATGA CTTTGTGGTG GGGTTCTGGA ATCCATCAGA AGAAAACTGT 
GGTGTTGACA CGGGAAAGCA GTCCATTTCT TACGACTTGC ACACTGAGCA GTGTATTGCT 
GACAAAAGCA TAGCGGACTG TGTGGAAGCC CTGCTGGGCT GCTATTTAAC CAGCTGTGGG 
GAGAGGGCTG CTCAGCTTTT CCTCTGTTCA CTGGGGCTGA AGGTGCTCCC GGTAATTAAA 
AGGACTGATC GGGAAAAGGC CCTGTGCCCT ACTCGGGAGA ATTTCAACAG CCAACAAAAG 
AACCTTTCAG TGAGCTGTGC TGCTGCTTCT GTGGCCAGTT CACGCTCTTC TGTATTGAAA 
GACTCGGAAT ATGGTTGTTT GAAGATTCCA CCAAGATGTA TGTTTGATCA TCCAGATGCA 
GATAAAACAC TGAATCACCT TATATCGGGG TTTGAAAATT TTGAAAAGAA AATCAACTAC 
AGATTCAAGA ATAAGGCTTA CCTTCTCCAG GCTTTTACAC ATGCCTCCTA CCACTACAAT 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCGCGG CAGCACTCCC CGGGGGTCCT GACAGACCTG 
CGGTCTGCCC TGGTCAACAA CACCATCTTT GCATCGCTGG CTGTAAAGTA CGACTACCAC 
AAGTACTTCA AAGCTGTCTC TCCTGAGCTC TTCCATGTCA TTGATGACTT TGTGCAGTTT 
CAGCTTGAGA AGAATGAAAT GCAAGGAATG GATTCTGAGC TTAGGAGATC TGAGGAGGAT 
GAAGAGAAAG AAGAGGATAT TGAAGTTCCA AAGGCCATGG GGGATATTTT TGAGTCGCTT 
GCTGGTGCCA TTTACATGGA TAGTGGGATG TCACTGGAGA CAGTCTGGCA GGTGTACTAT 
CCCATGATGC GGCCACTAAT AGAAAAGTTT TCTGCAAATG TACCCCGTTC CCCTGTGCGA 
GAATTGCTTG AAATGGAACC AGAAACTGCC AAATTTAGCC CGGCTGAGAG AACTTACGAC 
GGGAAGGTCA GAGTCACTGT GGAAGTAGTA GGAAAGGGGA AATTTAAAGG TGTTGGTCGA 
AGTTACAGGA TTGCCAAATC TGCAGCAGCA AGAAGAGCCC TCCGAAGCCT CAAAGCTAAT 
CAACCTCAGG TTCCCAATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876612.1
CDS	911..6274
Translation	MEKGRCSWSTLVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_017021123.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021123.2

ORF Insert Sequence:

ATGGAAAAAG GACGGTGTTC TTGGTCAACT CTGGTTCTCA TTATGACTTG CTATGTCGCC 
TTGAATGTTT TGAAAAATGG TTACTTATCA CTGTCAGACA TTAACCTTTT GGTGTTTGAT 
GAGTGTCATC TTGCAATCCT AGACCACCCC TATCGAGAAA TTATGAAGCT CTGTGAAAAT 
TGTCCATCAT GTCCTCGCAT TTTGGGACTA ACTGCTTCCA TTTTAAATGG GAAATGTGAT 
CCAGAGGAAT TGGAAGAAAA GATTCAGAAA CTAGAGAAAA TTCTTAAGAG TAATGCTGAA 
ACTGCAACTG ACCTGGTGGT CTTAGACAGG TATACTTCTC AGCCATGTGA GATTGTGGTG 
GATTGTGGAC CATTTACTGA CAGAAGTGGG CTTTATGAAA GACTGCTGAT GGAATTAGAA 
GAAGCACTTA ATTTTATCAA TGATTGTAAT ATATCTGTAC ATTCAAAAGA AAGAGATTCT 
ACTTTAATTT CGAAACAGAT ACTATCAGAC TGTCGTGCCG TATTGGTAGT TCTGGGACCC 
TGGTGTGCAG ATAAAGTAGC TGGAATGATG GTAAGAGAAC TACAGAAATA CATCAAACAT 
GAGCAAGAGG AGCTGCACAG GAAATTTTTA TTGTTTACAG ACACTTTCCT AAGGAAAATA 
CATGCACTAT GTGAAGAGCA CTTCTCACCT GCCTCACTTG ACCTGAAATT TGTAACTCCT 
AAAGTAATCA AACTGCTCGA AATCTTACGC AAATATAAAC CATATGAGCG ACAGCAGTTT 
GAAAGCGTTG AGTGGTATAA TAATAGAAAT CAGGATAATT ATGTGTCATG GAGTGATTCT 
GAGGATGATG ATGAGGATGA AGAAATTGAA GAAAAAGAGA AGCCAGAGAC AAATTTTCCT 
TCTCCTTTTA CCAACATTTT GTGCGGAATT ATTTTTGTGG AAAGAAGATA CACAGCAGTT 
GTCTTAAACA GATTGATAAA GGAAGCTGGC AAACAAGATC CAGAGCTGGC TTATATCAGT 
AGCAATTTCA TAACTGGACA TGGCATTGGG AAGAATCAGC CTCGCAACAA ACAGATGGAA 
GCAGAATTCA GAAAACAGGA AGAGGTACTT AGGAAATTTC GAGCACATGA GACCAACCTG 
CTTATTGCAA CAAGTATTGT AGAAGAGGGT GTTGATATAC CAAAATGCAA CTTGGTGGTT 
CGTTTTGATT TGCCCACAGA ATATCGATCC TATGTTCAAT CTAAAGGAAG AGCAAGGGCA 
CCCATCTCTA ATTATATAAT GTTAGCGGAT ACAGACAAAA TAAAAAGTTT TGAAGAAGAC 
CTTAAAACCT ACAAAGCTAT TGAAAAGATC TTGAGAAACA AGTGTTCCAA GTCGGTTGAT 
ACTGGTGAGA CTGACATTGA TCCTGTCATG GATGATGATG ACGTTTTCCC ACCATATGTG 
TTGAGGCCTG ACGATGGTGG TCCACGAGTC ACAATCAACA CGGCCATTGG ACACATCAAT 
AGATACTGTG CTAGATTACC AAGTGATCCG TTTACTCATC TAGCTCCTAA ATGCAGAACC 
CGAGAGTTGC CTGATGGTAC ATTTTATTCA ACTCTTTATC TGCCAATTAA CTCACCTCTT 
CGAGCCTCCA TTGTTGGTCC ACCAATGAGC TGTGTACGAT TGGCTGAAAG AGTTGTAGCT 
CTCATTTGCT GTGAGAAACT GCACAAAATT GGCGAACTGG ATGACCATTT GATGCCAGTT 
GGGAAAGAGA CTGTTAAATA TGAAGAGGAG CTTGATTTGC ATGATGAAGA AGAGACCAGT 
GTTCCAGGAA GACCAGGTTC CACGAAACGA AGGCAGTGCT ACCCAAAAGC AATTCCAGAG 
TGTTTGAGGG ATAGTTATCC CAGACCTGAT CAGCCCTGTT ACCTGTATGT GATAGGAATG 
GTTTTAACTA CACCTTTACC TGATGAACTC AACTTTAGAA GGCGGAAGCT CTATCCTCCT 
GAAGATACCA CAAGATGCTT TGGAATACTG ACGGCCAAAC CCATACCTCA GATTCCACAC 
TTTCCTGTGT ACACACGCTC TGGAGAGGTT ACCATATCCA TTGAGTTGAA GAAGTCTGGT 
TTCATGTTGT CTCTACAAAT GCTTGAGTTG ATTACAAGAC TTCACCAGTA TATATTCTCA 
CATATTCTTC GGCTTGAAAA ACCTGCACTA GAATTTAAAC CTACAGACGC TGATTCAGCA 
TACTGTGTTC TACCTCTTAA TGTTGTTAAT GACTCCAGCA CTTTGGATAT TGACTTTAAA 
TTCATGGAAG ATATTGAGAA GTCTGAAGCT CGCATAGGCA TTCCCAGTAC AAAGTATACA 
AAAGAAACAC CCTTTGTTTT TAAATTAGAA GATTACCAAG ATGCCGTTAT CATTCCAAGA 
TATCGCAATT TTGATCAGCC TCATCGATTT TATGTAGCTG ATGTGTACAC TGATCTTACC 
CCACTCAGTA AATTTCCTTC CCCTGAGTAT GAAACTTTTG CAGAATATTA TAAAACAAAG 
TACAACCTTG ACCTAACCAA TCTCAACCAG CCACTGCTGG ATGTGGACCA CACATCTTCA 
AGACTTAATC TTTTGACACC TCGACATTTG AATCAGAAGG GGAAAGCGCT TCCTTTAAGC 
AGTGCTGAGA AGAGGAAAGC CAAATGGGAA AGTCTGCAGA ATAAACAGAT ACTGGTTCCA 
GAACTCTGTG CTATACATCC AATTCCAGCA TCACTGTGGA GAAAAGCTGT TTGTCTCCCC 
AGCATACTTT ATCGCCTTCA CTGCCTTTTG ACTGCAGAGG AGCTAAGAGC CCAGACTGCC 
AGCGATGCTG GCGTGGGAGT CAGATCACTT CCTGCGGATT TTAGATACCC TAACTTAGAC 
TTCGGGTGGA AAAAATCTAT TGACAGCAAA TCTTTCATCT CAATTTCTAA CTCCTCTTCA 
GCTGAAAATG ATAATTACTG TAAGCACAGC ACAATTGTCC CTGAAAATGC TGCACATCAA 
GGTGCTAATA GAACCTCCTC TCTAGAAAAT CATGACCAAA TGTCTGTGAA CTGCAGAACG 
TTGCTCAGCG AGTCCCCTGG TAAGCTCCAC GTTGAAGTTT CAGCAGATCT TACAGCAATT 
AATGGTCTTT CTTACAATCA AAATCTCGCC AATGGCAGTT ATGATTTAGC TAACAGAGAC 
TTTTGCCAAG GAAATCAGCT AAATTACTAC AAGCAGGAAA TACCCGTGCA ACCAACTACC 
TCATATTCCA TTCAGAATTT ATACAGTTAC GAGAACCAGC CCCAGCCCAG CGATGAATGT 
ACTCTCCTGA GTAATAAATA CCTTGATGGA AATGCTAACA AATCTACCTC AGATGGAAGT 
CCTGTGATGG CCGTAATGCC TGGTACGACA GACACTATTC AAGTGCTCAA GGGCAGGATG 
GATTCTGAGC AGAGCCCTTC TATTGGGTAC TCCTCAAGGA CTCTTGGCCC CAATCCTGGA 
CTTATTCTTC AGGCTTTGAC TCTGTCAAAC GCTAGTGATG GATTTAACCT GGAGCGGCTT 
GAAATGCTTG GCGACTCCTT TTTAAAGCAT GCCATCACCA CATATCTATT TTGCACTTAC 
CCTGATGCGC ATGAGGGCCG CCTTTCATAT ATGAGAAGCA AAAAGGTCAG CAACTGTAAT 
CTGTATCGCC TTGGAAAAAA GAAGGGACTA CCCAGCCGCA TGGTGGTGTC AATATTTGAT 
CCCCCTGTGA ATTGGCTTCC TCCTGGTTAT GTAGTAAATC AAGACAAAAG CAACACAGAT 
AAATGGGAAA AAGATGAAAT GACAAAAGAC TGCATGCTGG CGAATGGCAA ACTGGATGAG 
GATTACGAGG AGGAGGATGA GGAGGAGGAG AGCCTGATGT GGAGGGCTCC GAAGGAAGAG 
GCTGACTATG AAGATGATTT CCTGGAGTAT GATCAGGAAC ATATCAGATT TATAGATAAT 
ATGTTAATGG GGTCAGGAGC TTTTGTAAAG AAAATCTCTC TTTCTCCTTT TTCAACCACT 
GATTCTGCAT ATGAATGGAA AATGCCCAAA AAATCCTCCT TAGGTAGTAT GCCATTTTCA 
TCAGATTTTG AGGATTTTGA CTACAGCTCT TGGGATGCAA TGTGCTATCT GGATCCTAGC 
AAAGCTGTTG AAGAAGATGA CTTTGTGGTG GGGTTCTGGA ATCCATCAGA AGAAAACTGT 
GGTGTTGACA CGGGAAAGCA GTCCATTTCT TACGACTTGC ACACTGAGCA GTGTATTGCT 
GACAAAAGCA TAGCGGACTG TGTGGAAGCC CTGCTGGGCT GCTATTTAAC CAGCTGTGGG 
GAGAGGGCTG CTCAGCTTTT CCTCTGTTCA CTGGGGCTGA AGGTGCTCCC GGTAATTAAA 
AGGACTGATC GGGAAAAGGC CCTGTGCCCT ACTCGGGAGA ATTTCAACAG CCAACAAAAG 
AACCTTTCAG TGAGCTGTGC TGCTGCTTCT GTGGCCAGTT CACGCTCTTC TGTATTGAAA 
GACTCGGAAT ATGGTTGTTT GAAGATTCCA CCAAGATGTA TGTTTGATCA TCCAGATGCA 
GATAAAACAC TGAATCACCT TATATCGGGG TTTGAAAATT TTGAAAAGAA AATCAACTAC 
AGATTCAAGA ATAAGGCTTA CCTTCTCCAG GCTTTTACAC ATGCCTCCTA CCACTACAAT 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCGCGG CAGCACTCCC CGGGGGTCCT GACAGACCTG 
CGGTCTGCCC TGGTCAACAA CACCATCTTT GCATCGCTGG CTGTAAAGTA CGACTACCAC 
AAGTACTTCA AAGCTGTCTC TCCTGAGCTC TTCCATGTCA TTGATGACTT TGTGCAGTTT 
CAGCTTGAGA AGAATGAAAT GCAAGGAATG GATTCTGAGC TTAGGAGATC TGAGGAGGAT 
GAAGAGAAAG AAGAGGATAT TGAAGTTCCA AAGGCCATGG GGGATATTTT TGAGTCGCTT 
GCTGGTGCCA TTTACATGGA TAGTGGGATG TCACTGGAGA CAGTCTGGCA GGTGTACTAT 
CCCATGATGC GGCCACTAAT AGAAAAGTTT TCTGCAAATG TACCCCGTTC CCCTGTGCGA 
GAATTGCTTG AAATGGAACC AGAAACTGCC AAATTTAGCC CGGCTGAGAG AACTTACGAC 
GGGAAGGTCA GAGTCACTGT GGAAGTAGTA GGAAAGGGGA AATTTAAAGG TGTTGGTCGA 
AGTTACAGGA TTGCCAAATC TGCAGCAGCA AGAAGAGCCC TCCGAAGCCT CAAAGCTAAT 
CAACCTCAGG TTCCCAATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_017021122.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876611.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X7, mRNA.

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CloneID	OHu66150
Clone ID Related Accession (Same CDS sequence)	XM_011536604.2 , XM_017021123.1 , XM_017021123.2 , XM_017021122.1 , XM_017021122.2
Accession Version	XM_017021122.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAG GACGGTGTTC TTGGTCAACT CTGGTTCTCA TTATGACTTG CTATGTCGCC 
TTGAATGTTT TGAAAAATGG TTACTTATCA CTGTCAGACA TTAACCTTTT GGTGTTTGAT 
GAGTGTCATC TTGCAATCCT AGACCACCCC TATCGAGAAA TTATGAAGCT CTGTGAAAAT 
TGTCCATCAT GTCCTCGCAT TTTGGGACTA ACTGCTTCCA TTTTAAATGG GAAATGTGAT 
CCAGAGGAAT TGGAAGAAAA GATTCAGAAA CTAGAGAAAA TTCTTAAGAG TAATGCTGAA 
ACTGCAACTG ACCTGGTGGT CTTAGACAGG TATACTTCTC AGCCATGTGA GATTGTGGTG 
GATTGTGGAC CATTTACTGA CAGAAGTGGG CTTTATGAAA GACTGCTGAT GGAATTAGAA 
GAAGCACTTA ATTTTATCAA TGATTGTAAT ATATCTGTAC ATTCAAAAGA AAGAGATTCT 
ACTTTAATTT CGAAACAGAT ACTATCAGAC TGTCGTGCCG TATTGGTAGT TCTGGGACCC 
TGGTGTGCAG ATAAAGTAGC TGGAATGATG GTAAGAGAAC TACAGAAATA CATCAAACAT 
GAGCAAGAGG AGCTGCACAG GAAATTTTTA TTGTTTACAG ACACTTTCCT AAGGAAAATA 
CATGCACTAT GTGAAGAGCA CTTCTCACCT GCCTCACTTG ACCTGAAATT TGTAACTCCT 
AAAGTAATCA AACTGCTCGA AATCTTACGC AAATATAAAC CATATGAGCG ACAGCAGTTT 
GAAAGCGTTG AGTGGTATAA TAATAGAAAT CAGGATAATT ATGTGTCATG GAGTGATTCT 
GAGGATGATG ATGAGGATGA AGAAATTGAA GAAAAAGAGA AGCCAGAGAC AAATTTTCCT 
TCTCCTTTTA CCAACATTTT GTGCGGAATT ATTTTTGTGG AAAGAAGATA CACAGCAGTT 
GTCTTAAACA GATTGATAAA GGAAGCTGGC AAACAAGATC CAGAGCTGGC TTATATCAGT 
AGCAATTTCA TAACTGGACA TGGCATTGGG AAGAATCAGC CTCGCAACAA ACAGATGGAA 
GCAGAATTCA GAAAACAGGA AGAGGTACTT AGGAAATTTC GAGCACATGA GACCAACCTG 
CTTATTGCAA CAAGTATTGT AGAAGAGGGT GTTGATATAC CAAAATGCAA CTTGGTGGTT 
CGTTTTGATT TGCCCACAGA ATATCGATCC TATGTTCAAT CTAAAGGAAG AGCAAGGGCA 
CCCATCTCTA ATTATATAAT GTTAGCGGAT ACAGACAAAA TAAAAAGTTT TGAAGAAGAC 
CTTAAAACCT ACAAAGCTAT TGAAAAGATC TTGAGAAACA AGTGTTCCAA GTCGGTTGAT 
ACTGGTGAGA CTGACATTGA TCCTGTCATG GATGATGATG ACGTTTTCCC ACCATATGTG 
TTGAGGCCTG ACGATGGTGG TCCACGAGTC ACAATCAACA CGGCCATTGG ACACATCAAT 
AGATACTGTG CTAGATTACC AAGTGATCCG TTTACTCATC TAGCTCCTAA ATGCAGAACC 
CGAGAGTTGC CTGATGGTAC ATTTTATTCA ACTCTTTATC TGCCAATTAA CTCACCTCTT 
CGAGCCTCCA TTGTTGGTCC ACCAATGAGC TGTGTACGAT TGGCTGAAAG AGTTGTAGCT 
CTCATTTGCT GTGAGAAACT GCACAAAATT GGCGAACTGG ATGACCATTT GATGCCAGTT 
GGGAAAGAGA CTGTTAAATA TGAAGAGGAG CTTGATTTGC ATGATGAAGA AGAGACCAGT 
GTTCCAGGAA GACCAGGTTC CACGAAACGA AGGCAGTGCT ACCCAAAAGC AATTCCAGAG 
TGTTTGAGGG ATAGTTATCC CAGACCTGAT CAGCCCTGTT ACCTGTATGT GATAGGAATG 
GTTTTAACTA CACCTTTACC TGATGAACTC AACTTTAGAA GGCGGAAGCT CTATCCTCCT 
GAAGATACCA CAAGATGCTT TGGAATACTG ACGGCCAAAC CCATACCTCA GATTCCACAC 
TTTCCTGTGT ACACACGCTC TGGAGAGGTT ACCATATCCA TTGAGTTGAA GAAGTCTGGT 
TTCATGTTGT CTCTACAAAT GCTTGAGTTG ATTACAAGAC TTCACCAGTA TATATTCTCA 
CATATTCTTC GGCTTGAAAA ACCTGCACTA GAATTTAAAC CTACAGACGC TGATTCAGCA 
TACTGTGTTC TACCTCTTAA TGTTGTTAAT GACTCCAGCA CTTTGGATAT TGACTTTAAA 
TTCATGGAAG ATATTGAGAA GTCTGAAGCT CGCATAGGCA TTCCCAGTAC AAAGTATACA 
AAAGAAACAC CCTTTGTTTT TAAATTAGAA GATTACCAAG ATGCCGTTAT CATTCCAAGA 
TATCGCAATT TTGATCAGCC TCATCGATTT TATGTAGCTG ATGTGTACAC TGATCTTACC 
CCACTCAGTA AATTTCCTTC CCCTGAGTAT GAAACTTTTG CAGAATATTA TAAAACAAAG 
TACAACCTTG ACCTAACCAA TCTCAACCAG CCACTGCTGG ATGTGGACCA CACATCTTCA 
AGACTTAATC TTTTGACACC TCGACATTTG AATCAGAAGG GGAAAGCGCT TCCTTTAAGC 
AGTGCTGAGA AGAGGAAAGC CAAATGGGAA AGTCTGCAGA ATAAACAGAT ACTGGTTCCA 
GAACTCTGTG CTATACATCC AATTCCAGCA TCACTGTGGA GAAAAGCTGT TTGTCTCCCC 
AGCATACTTT ATCGCCTTCA CTGCCTTTTG ACTGCAGAGG AGCTAAGAGC CCAGACTGCC 
AGCGATGCTG GCGTGGGAGT CAGATCACTT CCTGCGGATT TTAGATACCC TAACTTAGAC 
TTCGGGTGGA AAAAATCTAT TGACAGCAAA TCTTTCATCT CAATTTCTAA CTCCTCTTCA 
GCTGAAAATG ATAATTACTG TAAGCACAGC ACAATTGTCC CTGAAAATGC TGCACATCAA 
GGTGCTAATA GAACCTCCTC TCTAGAAAAT CATGACCAAA TGTCTGTGAA CTGCAGAACG 
TTGCTCAGCG AGTCCCCTGG TAAGCTCCAC GTTGAAGTTT CAGCAGATCT TACAGCAATT 
AATGGTCTTT CTTACAATCA AAATCTCGCC AATGGCAGTT ATGATTTAGC TAACAGAGAC 
TTTTGCCAAG GAAATCAGCT AAATTACTAC AAGCAGGAAA TACCCGTGCA ACCAACTACC 
TCATATTCCA TTCAGAATTT ATACAGTTAC GAGAACCAGC CCCAGCCCAG CGATGAATGT 
ACTCTCCTGA GTAATAAATA CCTTGATGGA AATGCTAACA AATCTACCTC AGATGGAAGT 
CCTGTGATGG CCGTAATGCC TGGTACGACA GACACTATTC AAGTGCTCAA GGGCAGGATG 
GATTCTGAGC AGAGCCCTTC TATTGGGTAC TCCTCAAGGA CTCTTGGCCC CAATCCTGGA 
CTTATTCTTC AGGCTTTGAC TCTGTCAAAC GCTAGTGATG GATTTAACCT GGAGCGGCTT 
GAAATGCTTG GCGACTCCTT TTTAAAGCAT GCCATCACCA CATATCTATT TTGCACTTAC 
CCTGATGCGC ATGAGGGCCG CCTTTCATAT ATGAGAAGCA AAAAGGTCAG CAACTGTAAT 
CTGTATCGCC TTGGAAAAAA GAAGGGACTA CCCAGCCGCA TGGTGGTGTC AATATTTGAT 
CCCCCTGTGA ATTGGCTTCC TCCTGGTTAT GTAGTAAATC AAGACAAAAG CAACACAGAT 
AAATGGGAAA AAGATGAAAT GACAAAAGAC TGCATGCTGG CGAATGGCAA ACTGGATGAG 
GATTACGAGG AGGAGGATGA GGAGGAGGAG AGCCTGATGT GGAGGGCTCC GAAGGAAGAG 
GCTGACTATG AAGATGATTT CCTGGAGTAT GATCAGGAAC ATATCAGATT TATAGATAAT 
ATGTTAATGG GGTCAGGAGC TTTTGTAAAG AAAATCTCTC TTTCTCCTTT TTCAACCACT 
GATTCTGCAT ATGAATGGAA AATGCCCAAA AAATCCTCCT TAGGTAGTAT GCCATTTTCA 
TCAGATTTTG AGGATTTTGA CTACAGCTCT TGGGATGCAA TGTGCTATCT GGATCCTAGC 
AAAGCTGTTG AAGAAGATGA CTTTGTGGTG GGGTTCTGGA ATCCATCAGA AGAAAACTGT 
GGTGTTGACA CGGGAAAGCA GTCCATTTCT TACGACTTGC ACACTGAGCA GTGTATTGCT 
GACAAAAGCA TAGCGGACTG TGTGGAAGCC CTGCTGGGCT GCTATTTAAC CAGCTGTGGG 
GAGAGGGCTG CTCAGCTTTT CCTCTGTTCA CTGGGGCTGA AGGTGCTCCC GGTAATTAAA 
AGGACTGATC GGGAAAAGGC CCTGTGCCCT ACTCGGGAGA ATTTCAACAG CCAACAAAAG 
AACCTTTCAG TGAGCTGTGC TGCTGCTTCT GTGGCCAGTT CACGCTCTTC TGTATTGAAA 
GACTCGGAAT ATGGTTGTTT GAAGATTCCA CCAAGATGTA TGTTTGATCA TCCAGATGCA 
GATAAAACAC TGAATCACCT TATATCGGGG TTTGAAAATT TTGAAAAGAA AATCAACTAC 
AGATTCAAGA ATAAGGCTTA CCTTCTCCAG GCTTTTACAC ATGCCTCCTA CCACTACAAT 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCGCGG CAGCACTCCC CGGGGGTCCT GACAGACCTG 
CGGTCTGCCC TGGTCAACAA CACCATCTTT GCATCGCTGG CTGTAAAGTA CGACTACCAC 
AAGTACTTCA AAGCTGTCTC TCCTGAGCTC TTCCATGTCA TTGATGACTT TGTGCAGTTT 
CAGCTTGAGA AGAATGAAAT GCAAGGAATG GATTCTGAGC TTAGGAGATC TGAGGAGGAT 
GAAGAGAAAG AAGAGGATAT TGAAGTTCCA AAGGCCATGG GGGATATTTT TGAGTCGCTT 
GCTGGTGCCA TTTACATGGA TAGTGGGATG TCACTGGAGA CAGTCTGGCA GGTGTACTAT 
CCCATGATGC GGCCACTAAT AGAAAAGTTT TCTGCAAATG TACCCCGTTC CCCTGTGCGA 
GAATTGCTTG AAATGGAACC AGAAACTGCC AAATTTAGCC CGGCTGAGAG AACTTACGAC 
GGGAAGGTCA GAGTCACTGT GGAAGTAGTA GGAAAGGGGA AATTTAAAGG TGTTGGTCGA 
AGTTACAGGA TTGCCAAATC TGCAGCAGCA AGAAGAGCCC TCCGAAGCCT CAAAGCTAAT 
CAACCTCAGG TTCCCAATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876611.1
CDS	576..5939
Translation	MEKGRCSWSTLVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_017021122.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021122.1

ORF Insert Sequence:

ATGGAAAAAG GACGGTGTTC TTGGTCAACT CTGGTTCTCA TTATGACTTG CTATGTCGCC 
TTGAATGTTT TGAAAAATGG TTACTTATCA CTGTCAGACA TTAACCTTTT GGTGTTTGAT 
GAGTGTCATC TTGCAATCCT AGACCACCCC TATCGAGAAA TTATGAAGCT CTGTGAAAAT 
TGTCCATCAT GTCCTCGCAT TTTGGGACTA ACTGCTTCCA TTTTAAATGG GAAATGTGAT 
CCAGAGGAAT TGGAAGAAAA GATTCAGAAA CTAGAGAAAA TTCTTAAGAG TAATGCTGAA 
ACTGCAACTG ACCTGGTGGT CTTAGACAGG TATACTTCTC AGCCATGTGA GATTGTGGTG 
GATTGTGGAC CATTTACTGA CAGAAGTGGG CTTTATGAAA GACTGCTGAT GGAATTAGAA 
GAAGCACTTA ATTTTATCAA TGATTGTAAT ATATCTGTAC ATTCAAAAGA AAGAGATTCT 
ACTTTAATTT CGAAACAGAT ACTATCAGAC TGTCGTGCCG TATTGGTAGT TCTGGGACCC 
TGGTGTGCAG ATAAAGTAGC TGGAATGATG GTAAGAGAAC TACAGAAATA CATCAAACAT 
GAGCAAGAGG AGCTGCACAG GAAATTTTTA TTGTTTACAG ACACTTTCCT AAGGAAAATA 
CATGCACTAT GTGAAGAGCA CTTCTCACCT GCCTCACTTG ACCTGAAATT TGTAACTCCT 
AAAGTAATCA AACTGCTCGA AATCTTACGC AAATATAAAC CATATGAGCG ACAGCAGTTT 
GAAAGCGTTG AGTGGTATAA TAATAGAAAT CAGGATAATT ATGTGTCATG GAGTGATTCT 
GAGGATGATG ATGAGGATGA AGAAATTGAA GAAAAAGAGA AGCCAGAGAC AAATTTTCCT 
TCTCCTTTTA CCAACATTTT GTGCGGAATT ATTTTTGTGG AAAGAAGATA CACAGCAGTT 
GTCTTAAACA GATTGATAAA GGAAGCTGGC AAACAAGATC CAGAGCTGGC TTATATCAGT 
AGCAATTTCA TAACTGGACA TGGCATTGGG AAGAATCAGC CTCGCAACAA ACAGATGGAA 
GCAGAATTCA GAAAACAGGA AGAGGTACTT AGGAAATTTC GAGCACATGA GACCAACCTG 
CTTATTGCAA CAAGTATTGT AGAAGAGGGT GTTGATATAC CAAAATGCAA CTTGGTGGTT 
CGTTTTGATT TGCCCACAGA ATATCGATCC TATGTTCAAT CTAAAGGAAG AGCAAGGGCA 
CCCATCTCTA ATTATATAAT GTTAGCGGAT ACAGACAAAA TAAAAAGTTT TGAAGAAGAC 
CTTAAAACCT ACAAAGCTAT TGAAAAGATC TTGAGAAACA AGTGTTCCAA GTCGGTTGAT 
ACTGGTGAGA CTGACATTGA TCCTGTCATG GATGATGATG ACGTTTTCCC ACCATATGTG 
TTGAGGCCTG ACGATGGTGG TCCACGAGTC ACAATCAACA CGGCCATTGG ACACATCAAT 
AGATACTGTG CTAGATTACC AAGTGATCCG TTTACTCATC TAGCTCCTAA ATGCAGAACC 
CGAGAGTTGC CTGATGGTAC ATTTTATTCA ACTCTTTATC TGCCAATTAA CTCACCTCTT 
CGAGCCTCCA TTGTTGGTCC ACCAATGAGC TGTGTACGAT TGGCTGAAAG AGTTGTAGCT 
CTCATTTGCT GTGAGAAACT GCACAAAATT GGCGAACTGG ATGACCATTT GATGCCAGTT 
GGGAAAGAGA CTGTTAAATA TGAAGAGGAG CTTGATTTGC ATGATGAAGA AGAGACCAGT 
GTTCCAGGAA GACCAGGTTC CACGAAACGA AGGCAGTGCT ACCCAAAAGC AATTCCAGAG 
TGTTTGAGGG ATAGTTATCC CAGACCTGAT CAGCCCTGTT ACCTGTATGT GATAGGAATG 
GTTTTAACTA CACCTTTACC TGATGAACTC AACTTTAGAA GGCGGAAGCT CTATCCTCCT 
GAAGATACCA CAAGATGCTT TGGAATACTG ACGGCCAAAC CCATACCTCA GATTCCACAC 
TTTCCTGTGT ACACACGCTC TGGAGAGGTT ACCATATCCA TTGAGTTGAA GAAGTCTGGT 
TTCATGTTGT CTCTACAAAT GCTTGAGTTG ATTACAAGAC TTCACCAGTA TATATTCTCA 
CATATTCTTC GGCTTGAAAA ACCTGCACTA GAATTTAAAC CTACAGACGC TGATTCAGCA 
TACTGTGTTC TACCTCTTAA TGTTGTTAAT GACTCCAGCA CTTTGGATAT TGACTTTAAA 
TTCATGGAAG ATATTGAGAA GTCTGAAGCT CGCATAGGCA TTCCCAGTAC AAAGTATACA 
AAAGAAACAC CCTTTGTTTT TAAATTAGAA GATTACCAAG ATGCCGTTAT CATTCCAAGA 
TATCGCAATT TTGATCAGCC TCATCGATTT TATGTAGCTG ATGTGTACAC TGATCTTACC 
CCACTCAGTA AATTTCCTTC CCCTGAGTAT GAAACTTTTG CAGAATATTA TAAAACAAAG 
TACAACCTTG ACCTAACCAA TCTCAACCAG CCACTGCTGG ATGTGGACCA CACATCTTCA 
AGACTTAATC TTTTGACACC TCGACATTTG AATCAGAAGG GGAAAGCGCT TCCTTTAAGC 
AGTGCTGAGA AGAGGAAAGC CAAATGGGAA AGTCTGCAGA ATAAACAGAT ACTGGTTCCA 
GAACTCTGTG CTATACATCC AATTCCAGCA TCACTGTGGA GAAAAGCTGT TTGTCTCCCC 
AGCATACTTT ATCGCCTTCA CTGCCTTTTG ACTGCAGAGG AGCTAAGAGC CCAGACTGCC 
AGCGATGCTG GCGTGGGAGT CAGATCACTT CCTGCGGATT TTAGATACCC TAACTTAGAC 
TTCGGGTGGA AAAAATCTAT TGACAGCAAA TCTTTCATCT CAATTTCTAA CTCCTCTTCA 
GCTGAAAATG ATAATTACTG TAAGCACAGC ACAATTGTCC CTGAAAATGC TGCACATCAA 
GGTGCTAATA GAACCTCCTC TCTAGAAAAT CATGACCAAA TGTCTGTGAA CTGCAGAACG 
TTGCTCAGCG AGTCCCCTGG TAAGCTCCAC GTTGAAGTTT CAGCAGATCT TACAGCAATT 
AATGGTCTTT CTTACAATCA AAATCTCGCC AATGGCAGTT ATGATTTAGC TAACAGAGAC 
TTTTGCCAAG GAAATCAGCT AAATTACTAC AAGCAGGAAA TACCCGTGCA ACCAACTACC 
TCATATTCCA TTCAGAATTT ATACAGTTAC GAGAACCAGC CCCAGCCCAG CGATGAATGT 
ACTCTCCTGA GTAATAAATA CCTTGATGGA AATGCTAACA AATCTACCTC AGATGGAAGT 
CCTGTGATGG CCGTAATGCC TGGTACGACA GACACTATTC AAGTGCTCAA GGGCAGGATG 
GATTCTGAGC AGAGCCCTTC TATTGGGTAC TCCTCAAGGA CTCTTGGCCC CAATCCTGGA 
CTTATTCTTC AGGCTTTGAC TCTGTCAAAC GCTAGTGATG GATTTAACCT GGAGCGGCTT 
GAAATGCTTG GCGACTCCTT TTTAAAGCAT GCCATCACCA CATATCTATT TTGCACTTAC 
CCTGATGCGC ATGAGGGCCG CCTTTCATAT ATGAGAAGCA AAAAGGTCAG CAACTGTAAT 
CTGTATCGCC TTGGAAAAAA GAAGGGACTA CCCAGCCGCA TGGTGGTGTC AATATTTGAT 
CCCCCTGTGA ATTGGCTTCC TCCTGGTTAT GTAGTAAATC AAGACAAAAG CAACACAGAT 
AAATGGGAAA AAGATGAAAT GACAAAAGAC TGCATGCTGG CGAATGGCAA ACTGGATGAG 
GATTACGAGG AGGAGGATGA GGAGGAGGAG AGCCTGATGT GGAGGGCTCC GAAGGAAGAG 
GCTGACTATG AAGATGATTT CCTGGAGTAT GATCAGGAAC ATATCAGATT TATAGATAAT 
ATGTTAATGG GGTCAGGAGC TTTTGTAAAG AAAATCTCTC TTTCTCCTTT TTCAACCACT 
GATTCTGCAT ATGAATGGAA AATGCCCAAA AAATCCTCCT TAGGTAGTAT GCCATTTTCA 
TCAGATTTTG AGGATTTTGA CTACAGCTCT TGGGATGCAA TGTGCTATCT GGATCCTAGC 
AAAGCTGTTG AAGAAGATGA CTTTGTGGTG GGGTTCTGGA ATCCATCAGA AGAAAACTGT 
GGTGTTGACA CGGGAAAGCA GTCCATTTCT TACGACTTGC ACACTGAGCA GTGTATTGCT 
GACAAAAGCA TAGCGGACTG TGTGGAAGCC CTGCTGGGCT GCTATTTAAC CAGCTGTGGG 
GAGAGGGCTG CTCAGCTTTT CCTCTGTTCA CTGGGGCTGA AGGTGCTCCC GGTAATTAAA 
AGGACTGATC GGGAAAAGGC CCTGTGCCCT ACTCGGGAGA ATTTCAACAG CCAACAAAAG 
AACCTTTCAG TGAGCTGTGC TGCTGCTTCT GTGGCCAGTT CACGCTCTTC TGTATTGAAA 
GACTCGGAAT ATGGTTGTTT GAAGATTCCA CCAAGATGTA TGTTTGATCA TCCAGATGCA 
GATAAAACAC TGAATCACCT TATATCGGGG TTTGAAAATT TTGAAAAGAA AATCAACTAC 
AGATTCAAGA ATAAGGCTTA CCTTCTCCAG GCTTTTACAC ATGCCTCCTA CCACTACAAT 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCGCGG CAGCACTCCC CGGGGGTCCT GACAGACCTG 
CGGTCTGCCC TGGTCAACAA CACCATCTTT GCATCGCTGG CTGTAAAGTA CGACTACCAC 
AAGTACTTCA AAGCTGTCTC TCCTGAGCTC TTCCATGTCA TTGATGACTT TGTGCAGTTT 
CAGCTTGAGA AGAATGAAAT GCAAGGAATG GATTCTGAGC TTAGGAGATC TGAGGAGGAT 
GAAGAGAAAG AAGAGGATAT TGAAGTTCCA AAGGCCATGG GGGATATTTT TGAGTCGCTT 
GCTGGTGCCA TTTACATGGA TAGTGGGATG TCACTGGAGA CAGTCTGGCA GGTGTACTAT 
CCCATGATGC GGCCACTAAT AGAAAAGTTT TCTGCAAATG TACCCCGTTC CCCTGTGCGA 
GAATTGCTTG AAATGGAACC AGAAACTGCC AAATTTAGCC CGGCTGAGAG AACTTACGAC 
GGGAAGGTCA GAGTCACTGT GGAAGTAGTA GGAAAGGGGA AATTTAAAGG TGTTGGTCGA 
AGTTACAGGA TTGCCAAATC TGCAGCAGCA AGAAGAGCCC TCCGAAGCCT CAAAGCTAAT 
CAACCTCAGG TTCCCAATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_017021122.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876611.1 Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X7, mRNA.

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CloneID	OHu66150
Clone ID Related Accession (Same CDS sequence)	XM_011536604.2 , XM_017021123.1 , XM_017021123.2 , XM_017021122.1 , XM_017021122.2
Accession Version	XM_017021122.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoribonuclease Dicer isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021122.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAG GACGGTGTTC TTGGTCAACT CTGGTTCTCA TTATGACTTG CTATGTCGCC 
TTGAATGTTT TGAAAAATGG TTACTTATCA CTGTCAGACA TTAACCTTTT GGTGTTTGAT 
GAGTGTCATC TTGCAATCCT AGACCACCCC TATCGAGAAA TTATGAAGCT CTGTGAAAAT 
TGTCCATCAT GTCCTCGCAT TTTGGGACTA ACTGCTTCCA TTTTAAATGG GAAATGTGAT 
CCAGAGGAAT TGGAAGAAAA GATTCAGAAA CTAGAGAAAA TTCTTAAGAG TAATGCTGAA 
ACTGCAACTG ACCTGGTGGT CTTAGACAGG TATACTTCTC AGCCATGTGA GATTGTGGTG 
GATTGTGGAC CATTTACTGA CAGAAGTGGG CTTTATGAAA GACTGCTGAT GGAATTAGAA 
GAAGCACTTA ATTTTATCAA TGATTGTAAT ATATCTGTAC ATTCAAAAGA AAGAGATTCT 
ACTTTAATTT CGAAACAGAT ACTATCAGAC TGTCGTGCCG TATTGGTAGT TCTGGGACCC 
TGGTGTGCAG ATAAAGTAGC TGGAATGATG GTAAGAGAAC TACAGAAATA CATCAAACAT 
GAGCAAGAGG AGCTGCACAG GAAATTTTTA TTGTTTACAG ACACTTTCCT AAGGAAAATA 
CATGCACTAT GTGAAGAGCA CTTCTCACCT GCCTCACTTG ACCTGAAATT TGTAACTCCT 
AAAGTAATCA AACTGCTCGA AATCTTACGC AAATATAAAC CATATGAGCG ACAGCAGTTT 
GAAAGCGTTG AGTGGTATAA TAATAGAAAT CAGGATAATT ATGTGTCATG GAGTGATTCT 
GAGGATGATG ATGAGGATGA AGAAATTGAA GAAAAAGAGA AGCCAGAGAC AAATTTTCCT 
TCTCCTTTTA CCAACATTTT GTGCGGAATT ATTTTTGTGG AAAGAAGATA CACAGCAGTT 
GTCTTAAACA GATTGATAAA GGAAGCTGGC AAACAAGATC CAGAGCTGGC TTATATCAGT 
AGCAATTTCA TAACTGGACA TGGCATTGGG AAGAATCAGC CTCGCAACAA ACAGATGGAA 
GCAGAATTCA GAAAACAGGA AGAGGTACTT AGGAAATTTC GAGCACATGA GACCAACCTG 
CTTATTGCAA CAAGTATTGT AGAAGAGGGT GTTGATATAC CAAAATGCAA CTTGGTGGTT 
CGTTTTGATT TGCCCACAGA ATATCGATCC TATGTTCAAT CTAAAGGAAG AGCAAGGGCA 
CCCATCTCTA ATTATATAAT GTTAGCGGAT ACAGACAAAA TAAAAAGTTT TGAAGAAGAC 
CTTAAAACCT ACAAAGCTAT TGAAAAGATC TTGAGAAACA AGTGTTCCAA GTCGGTTGAT 
ACTGGTGAGA CTGACATTGA TCCTGTCATG GATGATGATG ACGTTTTCCC ACCATATGTG 
TTGAGGCCTG ACGATGGTGG TCCACGAGTC ACAATCAACA CGGCCATTGG ACACATCAAT 
AGATACTGTG CTAGATTACC AAGTGATCCG TTTACTCATC TAGCTCCTAA ATGCAGAACC 
CGAGAGTTGC CTGATGGTAC ATTTTATTCA ACTCTTTATC TGCCAATTAA CTCACCTCTT 
CGAGCCTCCA TTGTTGGTCC ACCAATGAGC TGTGTACGAT TGGCTGAAAG AGTTGTAGCT 
CTCATTTGCT GTGAGAAACT GCACAAAATT GGCGAACTGG ATGACCATTT GATGCCAGTT 
GGGAAAGAGA CTGTTAAATA TGAAGAGGAG CTTGATTTGC ATGATGAAGA AGAGACCAGT 
GTTCCAGGAA GACCAGGTTC CACGAAACGA AGGCAGTGCT ACCCAAAAGC AATTCCAGAG 
TGTTTGAGGG ATAGTTATCC CAGACCTGAT CAGCCCTGTT ACCTGTATGT GATAGGAATG 
GTTTTAACTA CACCTTTACC TGATGAACTC AACTTTAGAA GGCGGAAGCT CTATCCTCCT 
GAAGATACCA CAAGATGCTT TGGAATACTG ACGGCCAAAC CCATACCTCA GATTCCACAC 
TTTCCTGTGT ACACACGCTC TGGAGAGGTT ACCATATCCA TTGAGTTGAA GAAGTCTGGT 
TTCATGTTGT CTCTACAAAT GCTTGAGTTG ATTACAAGAC TTCACCAGTA TATATTCTCA 
CATATTCTTC GGCTTGAAAA ACCTGCACTA GAATTTAAAC CTACAGACGC TGATTCAGCA 
TACTGTGTTC TACCTCTTAA TGTTGTTAAT GACTCCAGCA CTTTGGATAT TGACTTTAAA 
TTCATGGAAG ATATTGAGAA GTCTGAAGCT CGCATAGGCA TTCCCAGTAC AAAGTATACA 
AAAGAAACAC CCTTTGTTTT TAAATTAGAA GATTACCAAG ATGCCGTTAT CATTCCAAGA 
TATCGCAATT TTGATCAGCC TCATCGATTT TATGTAGCTG ATGTGTACAC TGATCTTACC 
CCACTCAGTA AATTTCCTTC CCCTGAGTAT GAAACTTTTG CAGAATATTA TAAAACAAAG 
TACAACCTTG ACCTAACCAA TCTCAACCAG CCACTGCTGG ATGTGGACCA CACATCTTCA 
AGACTTAATC TTTTGACACC TCGACATTTG AATCAGAAGG GGAAAGCGCT TCCTTTAAGC 
AGTGCTGAGA AGAGGAAAGC CAAATGGGAA AGTCTGCAGA ATAAACAGAT ACTGGTTCCA 
GAACTCTGTG CTATACATCC AATTCCAGCA TCACTGTGGA GAAAAGCTGT TTGTCTCCCC 
AGCATACTTT ATCGCCTTCA CTGCCTTTTG ACTGCAGAGG AGCTAAGAGC CCAGACTGCC 
AGCGATGCTG GCGTGGGAGT CAGATCACTT CCTGCGGATT TTAGATACCC TAACTTAGAC 
TTCGGGTGGA AAAAATCTAT TGACAGCAAA TCTTTCATCT CAATTTCTAA CTCCTCTTCA 
GCTGAAAATG ATAATTACTG TAAGCACAGC ACAATTGTCC CTGAAAATGC TGCACATCAA 
GGTGCTAATA GAACCTCCTC TCTAGAAAAT CATGACCAAA TGTCTGTGAA CTGCAGAACG 
TTGCTCAGCG AGTCCCCTGG TAAGCTCCAC GTTGAAGTTT CAGCAGATCT TACAGCAATT 
AATGGTCTTT CTTACAATCA AAATCTCGCC AATGGCAGTT ATGATTTAGC TAACAGAGAC 
TTTTGCCAAG GAAATCAGCT AAATTACTAC AAGCAGGAAA TACCCGTGCA ACCAACTACC 
TCATATTCCA TTCAGAATTT ATACAGTTAC GAGAACCAGC CCCAGCCCAG CGATGAATGT 
ACTCTCCTGA GTAATAAATA CCTTGATGGA AATGCTAACA AATCTACCTC AGATGGAAGT 
CCTGTGATGG CCGTAATGCC TGGTACGACA GACACTATTC AAGTGCTCAA GGGCAGGATG 
GATTCTGAGC AGAGCCCTTC TATTGGGTAC TCCTCAAGGA CTCTTGGCCC CAATCCTGGA 
CTTATTCTTC AGGCTTTGAC TCTGTCAAAC GCTAGTGATG GATTTAACCT GGAGCGGCTT 
GAAATGCTTG GCGACTCCTT TTTAAAGCAT GCCATCACCA CATATCTATT TTGCACTTAC 
CCTGATGCGC ATGAGGGCCG CCTTTCATAT ATGAGAAGCA AAAAGGTCAG CAACTGTAAT 
CTGTATCGCC TTGGAAAAAA GAAGGGACTA CCCAGCCGCA TGGTGGTGTC AATATTTGAT 
CCCCCTGTGA ATTGGCTTCC TCCTGGTTAT GTAGTAAATC AAGACAAAAG CAACACAGAT 
AAATGGGAAA AAGATGAAAT GACAAAAGAC TGCATGCTGG CGAATGGCAA ACTGGATGAG 
GATTACGAGG AGGAGGATGA GGAGGAGGAG AGCCTGATGT GGAGGGCTCC GAAGGAAGAG 
GCTGACTATG AAGATGATTT CCTGGAGTAT GATCAGGAAC ATATCAGATT TATAGATAAT 
ATGTTAATGG GGTCAGGAGC TTTTGTAAAG AAAATCTCTC TTTCTCCTTT TTCAACCACT 
GATTCTGCAT ATGAATGGAA AATGCCCAAA AAATCCTCCT TAGGTAGTAT GCCATTTTCA 
TCAGATTTTG AGGATTTTGA CTACAGCTCT TGGGATGCAA TGTGCTATCT GGATCCTAGC 
AAAGCTGTTG AAGAAGATGA CTTTGTGGTG GGGTTCTGGA ATCCATCAGA AGAAAACTGT 
GGTGTTGACA CGGGAAAGCA GTCCATTTCT TACGACTTGC ACACTGAGCA GTGTATTGCT 
GACAAAAGCA TAGCGGACTG TGTGGAAGCC CTGCTGGGCT GCTATTTAAC CAGCTGTGGG 
GAGAGGGCTG CTCAGCTTTT CCTCTGTTCA CTGGGGCTGA AGGTGCTCCC GGTAATTAAA 
AGGACTGATC GGGAAAAGGC CCTGTGCCCT ACTCGGGAGA ATTTCAACAG CCAACAAAAG 
AACCTTTCAG TGAGCTGTGC TGCTGCTTCT GTGGCCAGTT CACGCTCTTC TGTATTGAAA 
GACTCGGAAT ATGGTTGTTT GAAGATTCCA CCAAGATGTA TGTTTGATCA TCCAGATGCA 
GATAAAACAC TGAATCACCT TATATCGGGG TTTGAAAATT TTGAAAAGAA AATCAACTAC 
AGATTCAAGA ATAAGGCTTA CCTTCTCCAG GCTTTTACAC ATGCCTCCTA CCACTACAAT 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCGCGG CAGCACTCCC CGGGGGTCCT GACAGACCTG 
CGGTCTGCCC TGGTCAACAA CACCATCTTT GCATCGCTGG CTGTAAAGTA CGACTACCAC 
AAGTACTTCA AAGCTGTCTC TCCTGAGCTC TTCCATGTCA TTGATGACTT TGTGCAGTTT 
CAGCTTGAGA AGAATGAAAT GCAAGGAATG GATTCTGAGC TTAGGAGATC TGAGGAGGAT 
GAAGAGAAAG AAGAGGATAT TGAAGTTCCA AAGGCCATGG GGGATATTTT TGAGTCGCTT 
GCTGGTGCCA TTTACATGGA TAGTGGGATG TCACTGGAGA CAGTCTGGCA GGTGTACTAT 
CCCATGATGC GGCCACTAAT AGAAAAGTTT TCTGCAAATG TACCCCGTTC CCCTGTGCGA 
GAATTGCTTG AAATGGAACC AGAAACTGCC AAATTTAGCC CGGCTGAGAG AACTTACGAC 
GGGAAGGTCA GAGTCACTGT GGAAGTAGTA GGAAAGGGGA AATTTAAAGG TGTTGGTCGA 
AGTTACAGGA TTGCCAAATC TGCAGCAGCA AGAAGAGCCC TCCGAAGCCT CAAAGCTAAT 
CAACCTCAGG TTCCCAATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876611.1
CDS	1521..6884
Translation	MEKGRCSWSTLVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_017021122.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021122.2

ORF Insert Sequence:

ATGGAAAAAG GACGGTGTTC TTGGTCAACT CTGGTTCTCA TTATGACTTG CTATGTCGCC 
TTGAATGTTT TGAAAAATGG TTACTTATCA CTGTCAGACA TTAACCTTTT GGTGTTTGAT 
GAGTGTCATC TTGCAATCCT AGACCACCCC TATCGAGAAA TTATGAAGCT CTGTGAAAAT 
TGTCCATCAT GTCCTCGCAT TTTGGGACTA ACTGCTTCCA TTTTAAATGG GAAATGTGAT 
CCAGAGGAAT TGGAAGAAAA GATTCAGAAA CTAGAGAAAA TTCTTAAGAG TAATGCTGAA 
ACTGCAACTG ACCTGGTGGT CTTAGACAGG TATACTTCTC AGCCATGTGA GATTGTGGTG 
GATTGTGGAC CATTTACTGA CAGAAGTGGG CTTTATGAAA GACTGCTGAT GGAATTAGAA 
GAAGCACTTA ATTTTATCAA TGATTGTAAT ATATCTGTAC ATTCAAAAGA AAGAGATTCT 
ACTTTAATTT CGAAACAGAT ACTATCAGAC TGTCGTGCCG TATTGGTAGT TCTGGGACCC 
TGGTGTGCAG ATAAAGTAGC TGGAATGATG GTAAGAGAAC TACAGAAATA CATCAAACAT 
GAGCAAGAGG AGCTGCACAG GAAATTTTTA TTGTTTACAG ACACTTTCCT AAGGAAAATA 
CATGCACTAT GTGAAGAGCA CTTCTCACCT GCCTCACTTG ACCTGAAATT TGTAACTCCT 
AAAGTAATCA AACTGCTCGA AATCTTACGC AAATATAAAC CATATGAGCG ACAGCAGTTT 
GAAAGCGTTG AGTGGTATAA TAATAGAAAT CAGGATAATT ATGTGTCATG GAGTGATTCT 
GAGGATGATG ATGAGGATGA AGAAATTGAA GAAAAAGAGA AGCCAGAGAC AAATTTTCCT 
TCTCCTTTTA CCAACATTTT GTGCGGAATT ATTTTTGTGG AAAGAAGATA CACAGCAGTT 
GTCTTAAACA GATTGATAAA GGAAGCTGGC AAACAAGATC CAGAGCTGGC TTATATCAGT 
AGCAATTTCA TAACTGGACA TGGCATTGGG AAGAATCAGC CTCGCAACAA ACAGATGGAA 
GCAGAATTCA GAAAACAGGA AGAGGTACTT AGGAAATTTC GAGCACATGA GACCAACCTG 
CTTATTGCAA CAAGTATTGT AGAAGAGGGT GTTGATATAC CAAAATGCAA CTTGGTGGTT 
CGTTTTGATT TGCCCACAGA ATATCGATCC TATGTTCAAT CTAAAGGAAG AGCAAGGGCA 
CCCATCTCTA ATTATATAAT GTTAGCGGAT ACAGACAAAA TAAAAAGTTT TGAAGAAGAC 
CTTAAAACCT ACAAAGCTAT TGAAAAGATC TTGAGAAACA AGTGTTCCAA GTCGGTTGAT 
ACTGGTGAGA CTGACATTGA TCCTGTCATG GATGATGATG ACGTTTTCCC ACCATATGTG 
TTGAGGCCTG ACGATGGTGG TCCACGAGTC ACAATCAACA CGGCCATTGG ACACATCAAT 
AGATACTGTG CTAGATTACC AAGTGATCCG TTTACTCATC TAGCTCCTAA ATGCAGAACC 
CGAGAGTTGC CTGATGGTAC ATTTTATTCA ACTCTTTATC TGCCAATTAA CTCACCTCTT 
CGAGCCTCCA TTGTTGGTCC ACCAATGAGC TGTGTACGAT TGGCTGAAAG AGTTGTAGCT 
CTCATTTGCT GTGAGAAACT GCACAAAATT GGCGAACTGG ATGACCATTT GATGCCAGTT 
GGGAAAGAGA CTGTTAAATA TGAAGAGGAG CTTGATTTGC ATGATGAAGA AGAGACCAGT 
GTTCCAGGAA GACCAGGTTC CACGAAACGA AGGCAGTGCT ACCCAAAAGC AATTCCAGAG 
TGTTTGAGGG ATAGTTATCC CAGACCTGAT CAGCCCTGTT ACCTGTATGT GATAGGAATG 
GTTTTAACTA CACCTTTACC TGATGAACTC AACTTTAGAA GGCGGAAGCT CTATCCTCCT 
GAAGATACCA CAAGATGCTT TGGAATACTG ACGGCCAAAC CCATACCTCA GATTCCACAC 
TTTCCTGTGT ACACACGCTC TGGAGAGGTT ACCATATCCA TTGAGTTGAA GAAGTCTGGT 
TTCATGTTGT CTCTACAAAT GCTTGAGTTG ATTACAAGAC TTCACCAGTA TATATTCTCA 
CATATTCTTC GGCTTGAAAA ACCTGCACTA GAATTTAAAC CTACAGACGC TGATTCAGCA 
TACTGTGTTC TACCTCTTAA TGTTGTTAAT GACTCCAGCA CTTTGGATAT TGACTTTAAA 
TTCATGGAAG ATATTGAGAA GTCTGAAGCT CGCATAGGCA TTCCCAGTAC AAAGTATACA 
AAAGAAACAC CCTTTGTTTT TAAATTAGAA GATTACCAAG ATGCCGTTAT CATTCCAAGA 
TATCGCAATT TTGATCAGCC TCATCGATTT TATGTAGCTG ATGTGTACAC TGATCTTACC 
CCACTCAGTA AATTTCCTTC CCCTGAGTAT GAAACTTTTG CAGAATATTA TAAAACAAAG 
TACAACCTTG ACCTAACCAA TCTCAACCAG CCACTGCTGG ATGTGGACCA CACATCTTCA 
AGACTTAATC TTTTGACACC TCGACATTTG AATCAGAAGG GGAAAGCGCT TCCTTTAAGC 
AGTGCTGAGA AGAGGAAAGC CAAATGGGAA AGTCTGCAGA ATAAACAGAT ACTGGTTCCA 
GAACTCTGTG CTATACATCC AATTCCAGCA TCACTGTGGA GAAAAGCTGT TTGTCTCCCC 
AGCATACTTT ATCGCCTTCA CTGCCTTTTG ACTGCAGAGG AGCTAAGAGC CCAGACTGCC 
AGCGATGCTG GCGTGGGAGT CAGATCACTT CCTGCGGATT TTAGATACCC TAACTTAGAC 
TTCGGGTGGA AAAAATCTAT TGACAGCAAA TCTTTCATCT CAATTTCTAA CTCCTCTTCA 
GCTGAAAATG ATAATTACTG TAAGCACAGC ACAATTGTCC CTGAAAATGC TGCACATCAA 
GGTGCTAATA GAACCTCCTC TCTAGAAAAT CATGACCAAA TGTCTGTGAA CTGCAGAACG 
TTGCTCAGCG AGTCCCCTGG TAAGCTCCAC GTTGAAGTTT CAGCAGATCT TACAGCAATT 
AATGGTCTTT CTTACAATCA AAATCTCGCC AATGGCAGTT ATGATTTAGC TAACAGAGAC 
TTTTGCCAAG GAAATCAGCT AAATTACTAC AAGCAGGAAA TACCCGTGCA ACCAACTACC 
TCATATTCCA TTCAGAATTT ATACAGTTAC GAGAACCAGC CCCAGCCCAG CGATGAATGT 
ACTCTCCTGA GTAATAAATA CCTTGATGGA AATGCTAACA AATCTACCTC AGATGGAAGT 
CCTGTGATGG CCGTAATGCC TGGTACGACA GACACTATTC AAGTGCTCAA GGGCAGGATG 
GATTCTGAGC AGAGCCCTTC TATTGGGTAC TCCTCAAGGA CTCTTGGCCC CAATCCTGGA 
CTTATTCTTC AGGCTTTGAC TCTGTCAAAC GCTAGTGATG GATTTAACCT GGAGCGGCTT 
GAAATGCTTG GCGACTCCTT TTTAAAGCAT GCCATCACCA CATATCTATT TTGCACTTAC 
CCTGATGCGC ATGAGGGCCG CCTTTCATAT ATGAGAAGCA AAAAGGTCAG CAACTGTAAT 
CTGTATCGCC TTGGAAAAAA GAAGGGACTA CCCAGCCGCA TGGTGGTGTC AATATTTGAT 
CCCCCTGTGA ATTGGCTTCC TCCTGGTTAT GTAGTAAATC AAGACAAAAG CAACACAGAT 
AAATGGGAAA AAGATGAAAT GACAAAAGAC TGCATGCTGG CGAATGGCAA ACTGGATGAG 
GATTACGAGG AGGAGGATGA GGAGGAGGAG AGCCTGATGT GGAGGGCTCC GAAGGAAGAG 
GCTGACTATG AAGATGATTT CCTGGAGTAT GATCAGGAAC ATATCAGATT TATAGATAAT 
ATGTTAATGG GGTCAGGAGC TTTTGTAAAG AAAATCTCTC TTTCTCCTTT TTCAACCACT 
GATTCTGCAT ATGAATGGAA AATGCCCAAA AAATCCTCCT TAGGTAGTAT GCCATTTTCA 
TCAGATTTTG AGGATTTTGA CTACAGCTCT TGGGATGCAA TGTGCTATCT GGATCCTAGC 
AAAGCTGTTG AAGAAGATGA CTTTGTGGTG GGGTTCTGGA ATCCATCAGA AGAAAACTGT 
GGTGTTGACA CGGGAAAGCA GTCCATTTCT TACGACTTGC ACACTGAGCA GTGTATTGCT 
GACAAAAGCA TAGCGGACTG TGTGGAAGCC CTGCTGGGCT GCTATTTAAC CAGCTGTGGG 
GAGAGGGCTG CTCAGCTTTT CCTCTGTTCA CTGGGGCTGA AGGTGCTCCC GGTAATTAAA 
AGGACTGATC GGGAAAAGGC CCTGTGCCCT ACTCGGGAGA ATTTCAACAG CCAACAAAAG 
AACCTTTCAG TGAGCTGTGC TGCTGCTTCT GTGGCCAGTT CACGCTCTTC TGTATTGAAA 
GACTCGGAAT ATGGTTGTTT GAAGATTCCA CCAAGATGTA TGTTTGATCA TCCAGATGCA 
GATAAAACAC TGAATCACCT TATATCGGGG TTTGAAAATT TTGAAAAGAA AATCAACTAC 
AGATTCAAGA ATAAGGCTTA CCTTCTCCAG GCTTTTACAC ATGCCTCCTA CCACTACAAT 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCGCGG CAGCACTCCC CGGGGGTCCT GACAGACCTG 
CGGTCTGCCC TGGTCAACAA CACCATCTTT GCATCGCTGG CTGTAAAGTA CGACTACCAC 
AAGTACTTCA AAGCTGTCTC TCCTGAGCTC TTCCATGTCA TTGATGACTT TGTGCAGTTT 
CAGCTTGAGA AGAATGAAAT GCAAGGAATG GATTCTGAGC TTAGGAGATC TGAGGAGGAT 
GAAGAGAAAG AAGAGGATAT TGAAGTTCCA AAGGCCATGG GGGATATTTT TGAGTCGCTT 
GCTGGTGCCA TTTACATGGA TAGTGGGATG TCACTGGAGA CAGTCTGGCA GGTGTACTAT 
CCCATGATGC GGCCACTAAT AGAAAAGTTT TCTGCAAATG TACCCCGTTC CCCTGTGCGA 
GAATTGCTTG AAATGGAACC AGAAACTGCC AAATTTAGCC CGGCTGAGAG AACTTACGAC 
GGGAAGGTCA GAGTCACTGT GGAAGTAGTA GGAAAGGGGA AATTTAAAGG TGTTGGTCGA 
AGTTACAGGA TTGCCAAATC TGCAGCAGCA AGAAGAGCCC TCCGAAGCCT CAAAGCTAAT 
CAACCTCAGG TTCCCAATAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DICER1
Entrez Gene ID	23405
Full Name	dicer 1, ribonuclease III
Synonyms	DCR1,Dicer,Dicer1e,HERNA,K12H4.8-LIKE,MNG1,RMSE2
General protein information	Preferred Names dicer 1, ribonuclease III Names endoribonuclease Dicer Dicer1, Dcr-1 homolog dicer 1, double-stranded RNA-specific endoribonuclease dicer 1, ribonuclease type III helicase MOI helicase with RNAse motif
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.13
Summary	This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010].
Disorder MIM:	606241
Disorder Html:	Pleuropulmonary blastoma, 601200 (3); Goiter, multinodular 1, with or without Sertoli-Leydig cell tumors, 138800 (3); Rhabdomyosarcoma, embryonal, 2, 180295 (3)

Homo sapiens (human)	DICER1	NP_085124.2
Rattus norvegicus (Norway rat)	Dicer1	XP_006240573.1
Danio rerio (zebrafish)	dicer1	NP_001154925.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002836	XP_003436256.1
Magnaporthe oryzae (rice blast fungus)	MGG_01541	XP_003714515.1
Neurospora crassa	NCU08270	XP_961898.1
	DICER1	XP_001100868.1
Gallus gallus (chicken)	DICER1	NP_001035555.1
Drosophila melanogaster (fruit fly)	Dcr-1	NP_524453.1
Caenorhabditis elegans (roundworm)	dcr-1	NP_498761.2
Xenopus tropicalis (tropical clawed frog)	dicer1	NP_001123390.2
Canis lupus familiaris (dog)	DICER1	XP_868526.3
Bos taurus (cattle)	DICER1	NP_976235.1
Schizosaccharomyces pombe (fission yeast)	dcr1	NP_588215.2
Pan troglodytes (chimpanzee)	DICER1	XP_001154369.1
Mus musculus (house mouse)	Dicer1	NP_683750.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DICER1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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