dcr-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	dcr-1
Entrez Gene ID	176138
Full Name	Death-promoting deoxyribonuclease
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_066360.3	NP_498761.2	Death-promoting deoxyribonuclease
NM_066360.4	NP_498761.2	Death-promoting deoxyribonuclease

Pathways from BioSystems

Homologies

Homo sapiens (human)	DICER1	NP_085124.2
Rattus norvegicus (Norway rat)	Dicer1	XP_006240573.1
Danio rerio (zebrafish)	dicer1	NP_001154925.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002836	XP_003436256.1
Magnaporthe oryzae (rice blast fungus)	MGG_01541	XP_003714515.1
Neurospora crassa	NCU08270	XP_961898.1
	DICER1	XP_001100868.1
Gallus gallus (chicken)	DICER1	NP_001035555.1
Drosophila melanogaster (fruit fly)	Dcr-1	NP_524453.1
Caenorhabditis elegans (roundworm)	dcr-1	NP_498761.2
Xenopus tropicalis (tropical clawed frog)	dicer1	NP_001123390.2
Canis lupus familiaris (dog)	DICER1	XP_868526.3
Bos taurus (cattle)	DICER1	NP_976235.1
Schizosaccharomyces pombe (fission yeast)	dcr1	NP_588215.2
Pan troglodytes (chimpanzee)	DICER1	XP_001154369.1
Mus musculus (house mouse)	Dicer1	NP_683750.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report of transcriptome-wide map of Dicer targets; identify thousands of Dicer-binding sites in human cells and C. elegans; find known and hundreds of additional miRNAs; also report structural RNAs, promoter RNAs, and mitochondrial transcripts as Dicer targets; most Dicer-binding sites reside on mRNAs/lncRNAs and are not significantly processed into small RNAs.
Title: A variety of dicer substrates in human and C. elegans.

Cleavage of DCR-1 by caspase 3 may cause a conformational change that allows truncated DCR-1 to bind and process DNA, and may remove steric hindrance that blocks DNA access to truncated DCR-1.
Title: A novel mechanism underlies caspase-dependent conversion of the dicer ribonuclease into a deoxyribonuclease during apoptosis.

[review] Dicer-1 lacks a functional helicase domain and is responsible for processing pre-microRNAs into mature miRNAs.
Title: Dicer's role as an antiviral: still an enigma.

Taken together, these results uncover a previously unknown role for DCR-1/Dicer in C. elegans antibacterial immunity that is largely associated with miRNA processing.
Title: New role for DCR-1/dicer in Caenorhabditis elegans innate immunity against the highly virulent bacterium Bacillus thuringiensis DB27.

Results show that the tandem tudor protein ERI-5 potentiates ERI endo-RNAi by tethering an RNA-dependent RNA polymerase (RdRP, F10B5.7) module to DCR-1.
Title: Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi.

The following dcr-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dcr-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340045	NM_066360.4 Latest version!	Caenorhabditis elegans Death-promoting deoxyribonuclease (dcr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa340045	NM_066360.3	Caenorhabditis elegans Death-promoting deoxyribonuclease (dcr-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dcr-1 ( NM_066360.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498761.2 Caenorhabditis elegans Death-promoting deoxyribonuclease (dcr-1), partial mRNA.

Price & Availability↑

CloneID	OCa340045
Clone ID Related Accession (Same CDS sequence)	NM_066360.3 , NM_066360.4
Accession Version	NM_066360.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Death-promoting deoxyribonuclease
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_066360.2. COMPLETENESS: incomplete on both ends.

ATGGTCAGGG TAAGAGCTGA TTTACAATGT TTTAACCCCA GGGACTACCA GGTGGAACTT 
TTGGATAAAG CGACAAAAAA GAACACAATC GTCCAACTAG GCACTGGTTC AGGAAAAACT 
TTCATTGCAG TTCTACTGCT GAAAGAATAT GGAGTTCAAT TGTTTGCACC ATTGGATCAG 
GGTGGAAAAC GAGCGTTTTT TGTTGTAGAG AAAGTAAACC TCGTTGAACA ACAAGCTATC 
CACATCGAAG TTCACACAAG TTTCAAAGTT GGTCAGGTCC ACGGACAGAC AAGTAGTGGT 
TTATGGGACA GCAAAGAACA ATGTGATCAG TTCATGAAGA GGCACCATGT TGTTGTGATC 
ACAGCTCAAT GCCTTCTTGA TTTGATTCGA CATGCGTACT TGAAAATTGA AGACATGTGT 
GTTCTGATCT TTGACGAATG TCATCATGCT CTCGGTTCTC AACATCCATA CAGAAGCATC 
ATGGTCGATT ATAAGTTATT GAAAAAGGAC AAACCCGTCC CACGTGTATT AGGACTCACA 
GCCAGTCTTA TCAAAGCGAA AGTTGCGCCG GAAAAACTAA TGGAACAGTT AAAAAAGTTG 
GAATCTGCGA TGGACAGTGT TATAGAGACA GCTAGTGATT TAGTCTCGCT CTCGAAATAC 
GGTGCGAAAC CCTATGAAGT TGTGATCATC TGCAAAGACT TTGAAATTGG GTGCCTGGGT 
ATTCCGAATT TCGACACAGT TATAGAAATA TTTGATGAAA CTGTAGCCTT TGTGAACACC 
ACAACAGAAT TTCATCCAGA TCTAGATTTG GATCCTCGTA GACCGATTAA AGATTCTCTC 
AAGACGACGC GAGCTGTTTT TCGTCAACTG GGACCTTGGG CGGCATGGCG AACGGCTCAA 
GTTTGGGAGA AAGAGTTGGG AAAAATTATA AAATCTCAAG TGCTACCGGA TAAGACGTTG 
CGGTTTTTGA ATATGGCAAA AACATCTATG ATTACGATTA AAAGATTGTT GGAACCGGAG 
ATGAAGAAAA TCAAAAGTAT TGAAGCTCTC CGTCCATATG TTCCTCAACG TGTCATACGT 
CTTTTCGAAA TCCTAGAAAC TTTCAACCCC GAATTTCAAA AAGAACGAAT GAAGTTAGAA 
AAAGCAGAAC ATTTATCAGC CATTATTTTT GTCGATCAAC GGTACATTGC ATATTCGTTA 
CTTCTTATGA TGCGTCATAT TAAATCATGG GAGCCTAAGT TCAAGTTTGT GAACCCGGAT 
TACGTCGTTG GAGCCAGCGG ACGAAATTTG GCGAGTTCGG ATAGTCAAGG ATTACATAAA 
CGACAAACTG AAGTTCTTCG CCGTTTCCAT CGAAATGAAA TCAATTGTCT TATCGCTACA 
TCAGTGCTTG AAGAAGGTGT AGATGTTAAA CAATGTAATC TTGTGATTAA ATTTGATCGA 
CCACTTGACA TGAGATCATA CGTTCAGTCA AAGGGAAGAG CTCGGCGAGC TGGATCAAGA 
TATGTTATCA CTGTTGAGGA GAAAGATACT GCGGCGTGTG ATAGTGATTT GAAAGATTTC 
CAGCAAATAG AAAAAATTCT TCTTAGCCGC CATCGTACAG TCAACAATCC TATTGAAGAT 
GATTCGGATA GATTTGAAGA ATTTGATGTT GATTCTCAAA TGGAACCATA TGTTGTTGAA 
AAAACTGGTG CAACATTGAA AATGTCAACT GCTATCGCAT TAATTAATAG ATATTGTTCG 
AAGCTTCCTT CCGATATCTT CACTCGTCTT GTTCCACACA ATCAAATCAT TCCAATTGAA 
GAGAATGGAG TGACCAAATA CTGTGCAGAG CTTCTTCTTC CAATTAACTC ACCGATCAAA 
CATGCAATTG TGCTCAAAAA TCCAATGCCA AATAAGAAGA CTGCACAGAT GGCAGTTGCA 
CTTGAAGCAT GTCGACAATT ACATTTAGAA GGAGAACTCG ATGATAATTT ACTTCCAAAA 
GGAAGAGAGT CGATTGCCAA ATTATTAGAA CATATTGATG AGGAGCCGGA TGAGTATGCA 
CCTGGAATTG CTGCTAAAGT TGGATCATCA AAACGAAAGC AATTATATGA CAAAAAGATT 
GCTCGTGCTC TCAATGAATC CTTCGTGGAA GCAGATAAGG AATGTTTCAT CTATGCATTC 
GAATTGGAAC GTTTCCGGGA AGCCGAACTT ACGTTGAACC CGAAAAGACG AAAGTTTGAA 
GATCCATTTA ATTATGAATA TTGCTTCGGA TTTCTGAGCG CAAAAGAGAT ACCAAAGATC 
CCACCATTCC CAGTGTTTCT TCGACAAGGA AATATGAAAG TCCGATTGAT TGTCGCACCG 
AAAAAGACTA CCGTAACCGC TGCTCAGCTT CAAGAGATTC AACTGTTCCA TAATTATTTG 
TTCACACAAG TTCTTCAAAT GTGTAAAACG GGAAATTTGG AATTCGATGG AACATCAAAT 
GCACCATTGA ACACGTTGAT TGTTCCACTT AACAAGCGAA AAGATGATAT GTCCTACACA 
ATCAACATGA AATATGTATC GGAAGTAGTT GCAAACATGG AAAATATGCC ACGTATTCCA 
AAAGATGAAG TTCGTCGTCA ATACAAATTC AACGCTGAAG ATTATAAGGA TGCGATCGTA 
ATGCCGTGGT ATCGAAATCT GGAACAGCCT GTATTCTACT ATGTTGCTGA AATTCTACCA 
GAATGGAGGC CGTCGTCGAA ATTCCCGGAT ACGCACTTCG AAACGTTTAA CGAGTATTTC 
ATCAAGAAAT ATAAATTAGA GATTTATGAT CAGAATCAAA GTTTATTGGA TGTAGATTTC 
ACATCTACTC GTCTTAACCT TCTTCAACCT CGAATTCAAA ATCAACCACG TCGTTCAAGA 
ACTGTGAGTA ACTCCTCAAC GTCAAATATT CCTCAAGCAT CTGCTTCTGA TTCAAAAGAG 
TCGAATACTT CCGTTCCACA CTCTTCACAA CGTCAGATCC TTGTACCGGA ATTGATGGAC 
ATTCATCCTA TCTCTGCGAC TCTTTGGAAT GTTATAGCAG CCCTGCCAAG TATTTTCTAT 
CGTGTTAATC AGTTATTGTT GACTGATGAA CTTCGAGAAA CAATTTTGGT GAAAGCTTTT 
GGAAAAGAGA AAACTAAACT GGATGATAAT GTTGAATGGA ATTCTCTGGC ATATGCAACC 
GAATATGAAG AGAAACAAAC GATAATCGTT AAGAAAATTC AACAATTGAG AGATTTAAAT 
CAAAAATCGA TTGAAGATCA AGAAAGAGAG ACGAGAGAAA ATGATAAGAT TGACGATGGT 
GAGGAGTTAT TCAATATTGG AGTTTGGGAT CCAGAAGAGG CAGTCAGAAT TGGTGTAGAA 
ATTAGTTCAA GAGATGATAG AATGGACGGT GAAGATCAGG ATACTGTAGG ACTAACACAA 
GGACTTCATG ATGGGAATAT ATCCGATGAA GACGATGAAC TGCCGTTTGT AATGCATGAT 
TACACAGCCA GGCTCACTTC TAACAGAAAT GGAATTGGTG CATGGTCAGG ATCTGAGTCT 
ATTGTTCCAA GCGGATGGGG AGATTGGGAT GGACCTGAAC CAGATAATAG TCCCATGCCT 
TTCCAAATTC TCGGTGGACC AGGTGGTTTG AATGTGCAGG CACTGATGGC TGATGTCGGA 
AGAGTTTTTG ATCCATCTAC TGCTTCGTCT TCCCTTTCTC AAACTGTACA AGAATCGACA 
GTTTCACCTC CAAAACAATT GACAAAAGAA GAAGAACAGT TTAAGAAACT TCAAAATGAC 
TTGCTGAAAC AAGCAAAAGA GCGACTTGAA GCTCTAGAAA TGTCAGAAGA TATGGAGAAA 
CCGCGAAGAT TGGAGGATAC TGTGAATTTA GAAGATTATG GAGATGATCA AGAAAATCAA 
GAGGATGAGA ATACACCAAC AAATTTCCCG AAAACCATAG ATGAAGAAAT TGAAGAGTTG 
TCAATTGGAG CCCGGAAAAA ACAAGAAATA GACGACAATG CAGCTAAAAC TGATGTACTC 
GAAAGGGAAA ACTGTGAAGT TTTGCCAGTT GCTATAAATG AAAAGTCTCG TTCATTCTCT 
TTTGAAAAAG AAAGTAAAGC AATAAATGGA CGACTTATAA GACAACGATC AGAGGAATAC 
GTGTCACATA TTGATTCAGA TATTGGATTA GGAGTATCTC CATGTCTTTT GTTAACTGCT 
CTTACTACTT CCAATGCTGC AGATGGAATG TCATTAGAGA GATTCGAAAC AATCGGAGAC 
TCTTTCTTGA AGTTTGCTAC TACCGATTAT CTCTATCACA CGTTGCTTGA TCAACATGAA 
GGAAAATTGT CGTTTGCACG TAGTAAAGAA GTTTCAAATT GCAATTTGTA TCGACTGGGC 
AAGAAATTGG GAATACCTCA ATTGATTGTT GCAAATAAAT TCGATGCTCA CGATTCTTGG 
CTGCCACCAT GCTATATTCC AACTTGCGAC TTCAAAGCTC CTAACACTGA TGATGCAGAA 
GAGAAAGATA ATGAAATTGA ACGAATTTTG GATGGACAAG TGATTGAAGA GAAACCTGAA 
AATAAAACTG GATGGGACAT TGGTGGTGAT GTCTCGAAAA GCACTACCGA TGGAATAGAG 
ACTATTACAT TCCCAAAGCA AGCCCGTGTT GGTAATGATG ATATCTCTCC ACTTCCTTAC 
AACTTGCTCA CCCAACAGCA TATTTCGGAT AAATCAATCG CTGACGCAGT CGAAGCTTTG 
ATCGGAGTAC ATTTGCTGAC GTTGGGACCT AATCCAACGC TGAAGGTGAT GAATTGGATG 
GGACTGAAAG TAATTCAAAA AGATCAGAAG AGTGACGTAC CTTCACCTCT TCTACGATTC 
ATTGACACTC CGACCAATCC GAATGCTTCT CTAAATTTCT TGAACAATTT GTGGCAGCAA 
TTCCAGTTTA CACAATTGGA AGAGAAAATT GGATATCGCT TCAAGGAACG TGCTTATCTT 
GTTCAAGCTT TCACACATGC TTCGTACATC AATAATCGTG TCACAGGATG CTATCAAAGA 
TTAGAATTCC TCGGAGACGC TGTTCTCGAC TATATGATCA CCAGATATTT GTTTGAAGAT 
TCTCGTCAAT ACAGTCCGGG TGTTCTCACT GATTTACGAT CAGCTCTCGT CAATAATACA 
ATTTTCGCGT CGTTAGCAGT GAAATTCGAG TTTCAAAAGC ACTTCATCGC TATGTGCCCA 
GGGCTTTATC ACATGATTGA GAAATTTGTT AAGCTGTGCT CGGAGCGCAA TTTCGATACT 
AATTTCAATG CTGAGATGTA TATGGTGACT ACTGAGGAAG AAATAGATGA AGGCCAAGAA 
GAGGATATTG AAGTTCCAAA AGCGATGGGT GACATATTTG AATCAGTAGC TGGCGCCATT 
TATCTCGACT CTGGACGTAA TTTGGATACC ACTTGGCAAG TGATTTTCCA CATGATGCGC 
GGGACTATTG AATTGTGCTG TGCGAACCCA CCAAGATCAC CGATTCGAGA GCTTATGGAG 
TTCGAGCAGA GCAAAGTTCG ATTCAGTAAA ATGGAACGAA TTCTCGAGAG TGGAAAAGTT 
CGAGTTACAG TCGAGGTTGT TAATAACATG AGATTCACTG GAATGGGAAG GAACTATCGA 
ATCGCGAAAG CTACTGCTGC GAAAAGAGCT TTGAAATACC TCCATCAAAT TGAGCAGCAA 
AGAAGACAAA GCCCATCATT AACAACTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498761.2
CDS	1..5733
Translation	MVRVRADLQCFNPRDYQVELLDKATKKNTIVQLGTGSGKTFIAVLLLKEYGVQLFAPLDQGGKRAFFVVEKVNLVEQQAIHIEVHTSFKVGQVHGQTSSGLWDSKEQCDQFMKRHHVVVITAQCLLDLIRHAYLKIEDMCVLIFDECHHALGSQHPYRSIMVDYKLLKKDKPVPRVLGLTASLIKAKVAPEKLMEQLKKLESAMDSVIETASDLVSLSKYGAKPYEVVIICKDFEIGCLGIPNFDTVIEIFDETVAFVNTTTEFHPDLDLDPRRPIKDSLKTTRAVFRQLGPWAAWRTAQVWEKELGKIIKSQVLPDKTLRFLNMAKTSMITIKRLLEPEMKKIKSIEALRPYVPQRVIRLFEILETFNPEFQKERMKLEKAEHLSAIIFVDQRYIAYSLLLMMRHIKSWEPKFKFVNPDYVVGASGRNLASSDSQGLHKRQTEVLRRFHRNEINCLIATSVLEEGVDVKQCNLVIKFDRPLDMRSYVQSKGRARRAGSRYVITVEEKDTAACDSDLKDFQQIEKILLSRHRTVNNPIEDDSDRFEEFDVDSQMEPYVVEKTGATLKMSTAIALINRYCSKLPSDIFTRLVPHNQIIPIEENGVTKYCAELLLPINSPIKHAIVLKNPMPNKKTAQMAVALEACRQLHLEGELDDNLLPKGRESIAKLLEHIDEEPDEYAPGIAAKVGSSKRKQLYDKKIARALNESFVEADKECFIYAFELERFREAELTLNPKRRKFEDPFNYEYCFGFLSAKEIPKIPPFPVFLRQGNMKVRLIVAPKKTTVTAAQLQEIQLFHNYLFTQVLQMCKTGNLEFDGTSNAPLNTLIVPLNKRKDDMSYTINMKYVSEVVANMENMPRIPKDEVRRQYKFNAEDYKDAIVMPWYRNLEQPVFYYVAEILPEWRPSSKFPDTHFETFNEYFIKKYKLEIYDQNQSLLDVDFTSTRLNLLQPRIQNQPRRSRTVSNSSTSNIPQASASDSKESNTSVPHSSQRQILVPELMDIHPISATLWNVIAALPSIFYRVNQLLLTDELRETILVKAFGKEKTKLDDNVEWNSLAYATEYEEKQTIIVKKIQQLRDLNQKSIEDQERETRENDKIDDGEELFNIGVWDPEEAVRIGVEISSRDDRMDGEDQDTVGLTQGLHDGNISDEDDELPFVMHDYTARLTSNRNGIGAWSGSESIVPSGWGDWDGPEPDNSPMPFQILGGPGGLNVQALMADVGRVFDPSTASSSLSQTVQESTVSPPKQLTKEEEQFKKLQNDLLKQAKERLEALEMSEDMEKPRRLEDTVNLEDYGDDQENQEDENTPTNFPKTIDEEIEELSIGARKKQEIDDNAAKTDVLERENCEVLPVAINEKSRSFSFEKESKAINGRLIRQRSEEYVSHIDSDIGLGVSPCLLLTALTTSNAADGMSLERFETIGDSFLKFATTDYLYHTLLDQHEGKLSFARSKEVSNCNLYRLGKKLGIPQLIVANKFDAHDSWLPPCYIPTCDFKAPNTDDAEEKDNEIERILDGQVIEEKPENKTGWDIGGDVSKSTTDGIETITFPKQARVGNDDISPLPYNLLTQQHISDKSIADAVEALIGVHLLTLGPNPTLKVMNWMGLKVIQKDQKSDVPSPLLRFIDTPTNPNASLNFLNNLWQQFQFTQLEEKIGYRFKERAYLVQAFTHASYINNRVTGCYQRLEFLGDAVLDYMITRYLFEDSRQYSPGVLTDLRSALVNNTIFASLAVKFEFQKHFIAMCPGLYHMIEKFVKLCSERNFDTNFNAEMYMVTTEEEIDEGQEEDIEVPKAMGDIFESVAGAIYLDSGRNLDTTWQVIFHMMRGTIELCCANPPRSPIRELMEFEQSKVRFSKMERILESGKVRVTVEVVNNMRFTGMGRNYRIAKATAAKRALKYLHQIEQQRRQSPSLTTV

Target ORF information:

RefSeq Version	NM_066360.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Death-promoting deoxyribonuclease (dcr-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066360.3

ORF Insert Sequence:

ATGGTCAGGG TAAGAGCTGA TTTACAATGT TTTAACCCCA GGGACTACCA GGTGGAACTT 
TTGGATAAAG CGACAAAAAA GAACACAATC GTCCAACTAG GCACTGGTTC AGGAAAAACT 
TTCATTGCAG TTCTACTGCT GAAAGAATAT GGAGTTCAAT TGTTTGCACC ATTGGATCAG 
GGTGGAAAAC GAGCGTTTTT TGTTGTAGAG AAAGTAAACC TCGTTGAACA ACAAGCTATC 
CACATCGAAG TTCACACAAG TTTCAAAGTT GGTCAGGTCC ACGGACAGAC AAGTAGTGGT 
TTATGGGACA GCAAAGAACA ATGTGATCAG TTCATGAAGA GGCACCATGT TGTTGTGATC 
ACAGCTCAAT GCCTTCTTGA TTTGATTCGA CATGCGTACT TGAAAATTGA AGACATGTGT 
GTTCTGATCT TTGACGAATG TCATCATGCT CTCGGTTCTC AACATCCATA CAGAAGCATC 
ATGGTCGATT ATAAGTTATT GAAAAAGGAC AAACCCGTCC CACGTGTATT AGGACTCACA 
GCCAGTCTTA TCAAAGCGAA AGTTGCGCCG GAAAAACTAA TGGAACAGTT AAAAAAGTTG 
GAATCTGCGA TGGACAGTGT TATAGAGACA GCTAGTGATT TAGTCTCGCT CTCGAAATAC 
GGTGCGAAAC CCTATGAAGT TGTGATCATC TGCAAAGACT TTGAAATTGG GTGCCTGGGT 
ATTCCGAATT TCGACACAGT TATAGAAATA TTTGATGAAA CTGTAGCCTT TGTGAACACC 
ACAACAGAAT TTCATCCAGA TCTAGATTTG GATCCTCGTA GACCGATTAA AGATTCTCTC 
AAGACGACGC GAGCTGTTTT TCGTCAACTG GGACCTTGGG CGGCATGGCG AACGGCTCAA 
GTTTGGGAGA AAGAGTTGGG AAAAATTATA AAATCTCAAG TGCTACCGGA TAAGACGTTG 
CGGTTTTTGA ATATGGCAAA AACATCTATG ATTACGATTA AAAGATTGTT GGAACCGGAG 
ATGAAGAAAA TCAAAAGTAT TGAAGCTCTC CGTCCATATG TTCCTCAACG TGTCATACGT 
CTTTTCGAAA TCCTAGAAAC TTTCAACCCC GAATTTCAAA AAGAACGAAT GAAGTTAGAA 
AAAGCAGAAC ATTTATCAGC CATTATTTTT GTCGATCAAC GGTACATTGC ATATTCGTTA 
CTTCTTATGA TGCGTCATAT TAAATCATGG GAGCCTAAGT TCAAGTTTGT GAACCCGGAT 
TACGTCGTTG GAGCCAGCGG ACGAAATTTG GCGAGTTCGG ATAGTCAAGG ATTACATAAA 
CGACAAACTG AAGTTCTTCG CCGTTTCCAT CGAAATGAAA TCAATTGTCT TATCGCTACA 
TCAGTGCTTG AAGAAGGTGT AGATGTTAAA CAATGTAATC TTGTGATTAA ATTTGATCGA 
CCACTTGACA TGAGATCATA CGTTCAGTCA AAGGGAAGAG CTCGGCGAGC TGGATCAAGA 
TATGTTATCA CTGTTGAGGA GAAAGATACT GCGGCGTGTG ATAGTGATTT GAAAGATTTC 
CAGCAAATAG AAAAAATTCT TCTTAGCCGC CATCGTACAG TCAACAATCC TATTGAAGAT 
GATTCGGATA GATTTGAAGA ATTTGATGTT GATTCTCAAA TGGAACCATA TGTTGTTGAA 
AAAACTGGTG CAACATTGAA AATGTCAACT GCTATCGCAT TAATTAATAG ATATTGTTCG 
AAGCTTCCTT CCGATATCTT CACTCGTCTT GTTCCACACA ATCAAATCAT TCCAATTGAA 
GAGAATGGAG TGACCAAATA CTGTGCAGAG CTTCTTCTTC CAATTAACTC ACCGATCAAA 
CATGCAATTG TGCTCAAAAA TCCAATGCCA AATAAGAAGA CTGCACAGAT GGCAGTTGCA 
CTTGAAGCAT GTCGACAATT ACATTTAGAA GGAGAACTCG ATGATAATTT ACTTCCAAAA 
GGAAGAGAGT CGATTGCCAA ATTATTAGAA CATATTGATG AGGAGCCGGA TGAGTATGCA 
CCTGGAATTG CTGCTAAAGT TGGATCATCA AAACGAAAGC AATTATATGA CAAAAAGATT 
GCTCGTGCTC TCAATGAATC CTTCGTGGAA GCAGATAAGG AATGTTTCAT CTATGCATTC 
GAATTGGAAC GTTTCCGGGA AGCCGAACTT ACGTTGAACC CGAAAAGACG AAAGTTTGAA 
GATCCATTTA ATTATGAATA TTGCTTCGGA TTTCTGAGCG CAAAAGAGAT ACCAAAGATC 
CCACCATTCC CAGTGTTTCT TCGACAAGGA AATATGAAAG TCCGATTGAT TGTCGCACCG 
AAAAAGACTA CCGTAACCGC TGCTCAGCTT CAAGAGATTC AACTGTTCCA TAATTATTTG 
TTCACACAAG TTCTTCAAAT GTGTAAAACG GGAAATTTGG AATTCGATGG AACATCAAAT 
GCACCATTGA ACACGTTGAT TGTTCCACTT AACAAGCGAA AAGATGATAT GTCCTACACA 
ATCAACATGA AATATGTATC GGAAGTAGTT GCAAACATGG AAAATATGCC ACGTATTCCA 
AAAGATGAAG TTCGTCGTCA ATACAAATTC AACGCTGAAG ATTATAAGGA TGCGATCGTA 
ATGCCGTGGT ATCGAAATCT GGAACAGCCT GTATTCTACT ATGTTGCTGA AATTCTACCA 
GAATGGAGGC CGTCGTCGAA ATTCCCGGAT ACGCACTTCG AAACGTTTAA CGAGTATTTC 
ATCAAGAAAT ATAAATTAGA GATTTATGAT CAGAATCAAA GTTTATTGGA TGTAGATTTC 
ACATCTACTC GTCTTAACCT TCTTCAACCT CGAATTCAAA ATCAACCACG TCGTTCAAGA 
ACTGTGAGTA ACTCCTCAAC GTCAAATATT CCTCAAGCAT CTGCTTCTGA TTCAAAAGAG 
TCGAATACTT CCGTTCCACA CTCTTCACAA CGTCAGATCC TTGTACCGGA ATTGATGGAC 
ATTCATCCTA TCTCTGCGAC TCTTTGGAAT GTTATAGCAG CCCTGCCAAG TATTTTCTAT 
CGTGTTAATC AGTTATTGTT GACTGATGAA CTTCGAGAAA CAATTTTGGT GAAAGCTTTT 
GGAAAAGAGA AAACTAAACT GGATGATAAT GTTGAATGGA ATTCTCTGGC ATATGCAACC 
GAATATGAAG AGAAACAAAC GATAATCGTT AAGAAAATTC AACAATTGAG AGATTTAAAT 
CAAAAATCGA TTGAAGATCA AGAAAGAGAG ACGAGAGAAA ATGATAAGAT TGACGATGGT 
GAGGAGTTAT TCAATATTGG AGTTTGGGAT CCAGAAGAGG CAGTCAGAAT TGGTGTAGAA 
ATTAGTTCAA GAGATGATAG AATGGACGGT GAAGATCAGG ATACTGTAGG ACTAACACAA 
GGACTTCATG ATGGGAATAT ATCCGATGAA GACGATGAAC TGCCGTTTGT AATGCATGAT 
TACACAGCCA GGCTCACTTC TAACAGAAAT GGAATTGGTG CATGGTCAGG ATCTGAGTCT 
ATTGTTCCAA GCGGATGGGG AGATTGGGAT GGACCTGAAC CAGATAATAG TCCCATGCCT 
TTCCAAATTC TCGGTGGACC AGGTGGTTTG AATGTGCAGG CACTGATGGC TGATGTCGGA 
AGAGTTTTTG ATCCATCTAC TGCTTCGTCT TCCCTTTCTC AAACTGTACA AGAATCGACA 
GTTTCACCTC CAAAACAATT GACAAAAGAA GAAGAACAGT TTAAGAAACT TCAAAATGAC 
TTGCTGAAAC AAGCAAAAGA GCGACTTGAA GCTCTAGAAA TGTCAGAAGA TATGGAGAAA 
CCGCGAAGAT TGGAGGATAC TGTGAATTTA GAAGATTATG GAGATGATCA AGAAAATCAA 
GAGGATGAGA ATACACCAAC AAATTTCCCG AAAACCATAG ATGAAGAAAT TGAAGAGTTG 
TCAATTGGAG CCCGGAAAAA ACAAGAAATA GACGACAATG CAGCTAAAAC TGATGTACTC 
GAAAGGGAAA ACTGTGAAGT TTTGCCAGTT GCTATAAATG AAAAGTCTCG TTCATTCTCT 
TTTGAAAAAG AAAGTAAAGC AATAAATGGA CGACTTATAA GACAACGATC AGAGGAATAC 
GTGTCACATA TTGATTCAGA TATTGGATTA GGAGTATCTC CATGTCTTTT GTTAACTGCT 
CTTACTACTT CCAATGCTGC AGATGGAATG TCATTAGAGA GATTCGAAAC AATCGGAGAC 
TCTTTCTTGA AGTTTGCTAC TACCGATTAT CTCTATCACA CGTTGCTTGA TCAACATGAA 
GGAAAATTGT CGTTTGCACG TAGTAAAGAA GTTTCAAATT GCAATTTGTA TCGACTGGGC 
AAGAAATTGG GAATACCTCA ATTGATTGTT GCAAATAAAT TCGATGCTCA CGATTCTTGG 
CTGCCACCAT GCTATATTCC AACTTGCGAC TTCAAAGCTC CTAACACTGA TGATGCAGAA 
GAGAAAGATA ATGAAATTGA ACGAATTTTG GATGGACAAG TGATTGAAGA GAAACCTGAA 
AATAAAACTG GATGGGACAT TGGTGGTGAT GTCTCGAAAA GCACTACCGA TGGAATAGAG 
ACTATTACAT TCCCAAAGCA AGCCCGTGTT GGTAATGATG ATATCTCTCC ACTTCCTTAC 
AACTTGCTCA CCCAACAGCA TATTTCGGAT AAATCAATCG CTGACGCAGT CGAAGCTTTG 
ATCGGAGTAC ATTTGCTGAC GTTGGGACCT AATCCAACGC TGAAGGTGAT GAATTGGATG 
GGACTGAAAG TAATTCAAAA AGATCAGAAG AGTGACGTAC CTTCACCTCT TCTACGATTC 
ATTGACACTC CGACCAATCC GAATGCTTCT CTAAATTTCT TGAACAATTT GTGGCAGCAA 
TTCCAGTTTA CACAATTGGA AGAGAAAATT GGATATCGCT TCAAGGAACG TGCTTATCTT 
GTTCAAGCTT TCACACATGC TTCGTACATC AATAATCGTG TCACAGGATG CTATCAAAGA 
TTAGAATTCC TCGGAGACGC TGTTCTCGAC TATATGATCA CCAGATATTT GTTTGAAGAT 
TCTCGTCAAT ACAGTCCGGG TGTTCTCACT GATTTACGAT CAGCTCTCGT CAATAATACA 
ATTTTCGCGT CGTTAGCAGT GAAATTCGAG TTTCAAAAGC ACTTCATCGC TATGTGCCCA 
GGGCTTTATC ACATGATTGA GAAATTTGTT AAGCTGTGCT CGGAGCGCAA TTTCGATACT 
AATTTCAATG CTGAGATGTA TATGGTGACT ACTGAGGAAG AAATAGATGA AGGCCAAGAA 
GAGGATATTG AAGTTCCAAA AGCGATGGGT GACATATTTG AATCAGTAGC TGGCGCCATT 
TATCTCGACT CTGGACGTAA TTTGGATACC ACTTGGCAAG TGATTTTCCA CATGATGCGC 
GGGACTATTG AATTGTGCTG TGCGAACCCA CCAAGATCAC CGATTCGAGA GCTTATGGAG 
TTCGAGCAGA GCAAAGTTCG ATTCAGTAAA ATGGAACGAA TTCTCGAGAG TGGAAAAGTT 
CGAGTTACAG TCGAGGTTGT TAATAACATG AGATTCACTG GAATGGGAAG GAACTATCGA 
ATCGCGAAAG CTACTGCTGC GAAAAGAGCT TTGAAATACC TCCATCAAAT TGAGCAGCAA 
AGAAGACAAA GCCCATCATT AACAACTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dcr-1 ( NM_066360.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498761.2 Caenorhabditis elegans Death-promoting deoxyribonuclease (dcr-1), mRNA.

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CloneID	OCa340045
Clone ID Related Accession (Same CDS sequence)	NM_066360.3 , NM_066360.4
Accession Version	NM_066360.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Death-promoting deoxyribonuclease
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_066360.3.

ATGGTCAGGG TAAGAGCTGA TTTACAATGT TTTAACCCCA GGGACTACCA GGTGGAACTT 
TTGGATAAAG CGACAAAAAA GAACACAATC GTCCAACTAG GCACTGGTTC AGGAAAAACT 
TTCATTGCAG TTCTACTGCT GAAAGAATAT GGAGTTCAAT TGTTTGCACC ATTGGATCAG 
GGTGGAAAAC GAGCGTTTTT TGTTGTAGAG AAAGTAAACC TCGTTGAACA ACAAGCTATC 
CACATCGAAG TTCACACAAG TTTCAAAGTT GGTCAGGTCC ACGGACAGAC AAGTAGTGGT 
TTATGGGACA GCAAAGAACA ATGTGATCAG TTCATGAAGA GGCACCATGT TGTTGTGATC 
ACAGCTCAAT GCCTTCTTGA TTTGATTCGA CATGCGTACT TGAAAATTGA AGACATGTGT 
GTTCTGATCT TTGACGAATG TCATCATGCT CTCGGTTCTC AACATCCATA CAGAAGCATC 
ATGGTCGATT ATAAGTTATT GAAAAAGGAC AAACCCGTCC CACGTGTATT AGGACTCACA 
GCCAGTCTTA TCAAAGCGAA AGTTGCGCCG GAAAAACTAA TGGAACAGTT AAAAAAGTTG 
GAATCTGCGA TGGACAGTGT TATAGAGACA GCTAGTGATT TAGTCTCGCT CTCGAAATAC 
GGTGCGAAAC CCTATGAAGT TGTGATCATC TGCAAAGACT TTGAAATTGG GTGCCTGGGT 
ATTCCGAATT TCGACACAGT TATAGAAATA TTTGATGAAA CTGTAGCCTT TGTGAACACC 
ACAACAGAAT TTCATCCAGA TCTAGATTTG GATCCTCGTA GACCGATTAA AGATTCTCTC 
AAGACGACGC GAGCTGTTTT TCGTCAACTG GGACCTTGGG CGGCATGGCG AACGGCTCAA 
GTTTGGGAGA AAGAGTTGGG AAAAATTATA AAATCTCAAG TGCTACCGGA TAAGACGTTG 
CGGTTTTTGA ATATGGCAAA AACATCTATG ATTACGATTA AAAGATTGTT GGAACCGGAG 
ATGAAGAAAA TCAAAAGTAT TGAAGCTCTC CGTCCATATG TTCCTCAACG TGTCATACGT 
CTTTTCGAAA TCCTAGAAAC TTTCAACCCC GAATTTCAAA AAGAACGAAT GAAGTTAGAA 
AAAGCAGAAC ATTTATCAGC CATTATTTTT GTCGATCAAC GGTACATTGC ATATTCGTTA 
CTTCTTATGA TGCGTCATAT TAAATCATGG GAGCCTAAGT TCAAGTTTGT GAACCCGGAT 
TACGTCGTTG GAGCCAGCGG ACGAAATTTG GCGAGTTCGG ATAGTCAAGG ATTACATAAA 
CGACAAACTG AAGTTCTTCG CCGTTTCCAT CGAAATGAAA TCAATTGTCT TATCGCTACA 
TCAGTGCTTG AAGAAGGTGT AGATGTTAAA CAATGTAATC TTGTGATTAA ATTTGATCGA 
CCACTTGACA TGAGATCATA CGTTCAGTCA AAGGGAAGAG CTCGGCGAGC TGGATCAAGA 
TATGTTATCA CTGTTGAGGA GAAAGATACT GCGGCGTGTG ATAGTGATTT GAAAGATTTC 
CAGCAAATAG AAAAAATTCT TCTTAGCCGC CATCGTACAG TCAACAATCC TATTGAAGAT 
GATTCGGATA GATTTGAAGA ATTTGATGTT GATTCTCAAA TGGAACCATA TGTTGTTGAA 
AAAACTGGTG CAACATTGAA AATGTCAACT GCTATCGCAT TAATTAATAG ATATTGTTCG 
AAGCTTCCTT CCGATATCTT CACTCGTCTT GTTCCACACA ATCAAATCAT TCCAATTGAA 
GAGAATGGAG TGACCAAATA CTGTGCAGAG CTTCTTCTTC CAATTAACTC ACCGATCAAA 
CATGCAATTG TGCTCAAAAA TCCAATGCCA AATAAGAAGA CTGCACAGAT GGCAGTTGCA 
CTTGAAGCAT GTCGACAATT ACATTTAGAA GGAGAACTCG ATGATAATTT ACTTCCAAAA 
GGAAGAGAGT CGATTGCCAA ATTATTAGAA CATATTGATG AGGAGCCGGA TGAGTATGCA 
CCTGGAATTG CTGCTAAAGT TGGATCATCA AAACGAAAGC AATTATATGA CAAAAAGATT 
GCTCGTGCTC TCAATGAATC CTTCGTGGAA GCAGATAAGG AATGTTTCAT CTATGCATTC 
GAATTGGAAC GTTTCCGGGA AGCCGAACTT ACGTTGAACC CGAAAAGACG AAAGTTTGAA 
GATCCATTTA ATTATGAATA TTGCTTCGGA TTTCTGAGCG CAAAAGAGAT ACCAAAGATC 
CCACCATTCC CAGTGTTTCT TCGACAAGGA AATATGAAAG TCCGATTGAT TGTCGCACCG 
AAAAAGACTA CCGTAACCGC TGCTCAGCTT CAAGAGATTC AACTGTTCCA TAATTATTTG 
TTCACACAAG TTCTTCAAAT GTGTAAAACG GGAAATTTGG AATTCGATGG AACATCAAAT 
GCACCATTGA ACACGTTGAT TGTTCCACTT AACAAGCGAA AAGATGATAT GTCCTACACA 
ATCAACATGA AATATGTATC GGAAGTAGTT GCAAACATGG AAAATATGCC ACGTATTCCA 
AAAGATGAAG TTCGTCGTCA ATACAAATTC AACGCTGAAG ATTATAAGGA TGCGATCGTA 
ATGCCGTGGT ATCGAAATCT GGAACAGCCT GTATTCTACT ATGTTGCTGA AATTCTACCA 
GAATGGAGGC CGTCGTCGAA ATTCCCGGAT ACGCACTTCG AAACGTTTAA CGAGTATTTC 
ATCAAGAAAT ATAAATTAGA GATTTATGAT CAGAATCAAA GTTTATTGGA TGTAGATTTC 
ACATCTACTC GTCTTAACCT TCTTCAACCT CGAATTCAAA ATCAACCACG TCGTTCAAGA 
ACTGTGAGTA ACTCCTCAAC GTCAAATATT CCTCAAGCAT CTGCTTCTGA TTCAAAAGAG 
TCGAATACTT CCGTTCCACA CTCTTCACAA CGTCAGATCC TTGTACCGGA ATTGATGGAC 
ATTCATCCTA TCTCTGCGAC TCTTTGGAAT GTTATAGCAG CCCTGCCAAG TATTTTCTAT 
CGTGTTAATC AGTTATTGTT GACTGATGAA CTTCGAGAAA CAATTTTGGT GAAAGCTTTT 
GGAAAAGAGA AAACTAAACT GGATGATAAT GTTGAATGGA ATTCTCTGGC ATATGCAACC 
GAATATGAAG AGAAACAAAC GATAATCGTT AAGAAAATTC AACAATTGAG AGATTTAAAT 
CAAAAATCGA TTGAAGATCA AGAAAGAGAG ACGAGAGAAA ATGATAAGAT TGACGATGGT 
GAGGAGTTAT TCAATATTGG AGTTTGGGAT CCAGAAGAGG CAGTCAGAAT TGGTGTAGAA 
ATTAGTTCAA GAGATGATAG AATGGACGGT GAAGATCAGG ATACTGTAGG ACTAACACAA 
GGACTTCATG ATGGGAATAT ATCCGATGAA GACGATGAAC TGCCGTTTGT AATGCATGAT 
TACACAGCCA GGCTCACTTC TAACAGAAAT GGAATTGGTG CATGGTCAGG ATCTGAGTCT 
ATTGTTCCAA GCGGATGGGG AGATTGGGAT GGACCTGAAC CAGATAATAG TCCCATGCCT 
TTCCAAATTC TCGGTGGACC AGGTGGTTTG AATGTGCAGG CACTGATGGC TGATGTCGGA 
AGAGTTTTTG ATCCATCTAC TGCTTCGTCT TCCCTTTCTC AAACTGTACA AGAATCGACA 
GTTTCACCTC CAAAACAATT GACAAAAGAA GAAGAACAGT TTAAGAAACT TCAAAATGAC 
TTGCTGAAAC AAGCAAAAGA GCGACTTGAA GCTCTAGAAA TGTCAGAAGA TATGGAGAAA 
CCGCGAAGAT TGGAGGATAC TGTGAATTTA GAAGATTATG GAGATGATCA AGAAAATCAA 
GAGGATGAGA ATACACCAAC AAATTTCCCG AAAACCATAG ATGAAGAAAT TGAAGAGTTG 
TCAATTGGAG CCCGGAAAAA ACAAGAAATA GACGACAATG CAGCTAAAAC TGATGTACTC 
GAAAGGGAAA ACTGTGAAGT TTTGCCAGTT GCTATAAATG AAAAGTCTCG TTCATTCTCT 
TTTGAAAAAG AAAGTAAAGC AATAAATGGA CGACTTATAA GACAACGATC AGAGGAATAC 
GTGTCACATA TTGATTCAGA TATTGGATTA GGAGTATCTC CATGTCTTTT GTTAACTGCT 
CTTACTACTT CCAATGCTGC AGATGGAATG TCATTAGAGA GATTCGAAAC AATCGGAGAC 
TCTTTCTTGA AGTTTGCTAC TACCGATTAT CTCTATCACA CGTTGCTTGA TCAACATGAA 
GGAAAATTGT CGTTTGCACG TAGTAAAGAA GTTTCAAATT GCAATTTGTA TCGACTGGGC 
AAGAAATTGG GAATACCTCA ATTGATTGTT GCAAATAAAT TCGATGCTCA CGATTCTTGG 
CTGCCACCAT GCTATATTCC AACTTGCGAC TTCAAAGCTC CTAACACTGA TGATGCAGAA 
GAGAAAGATA ATGAAATTGA ACGAATTTTG GATGGACAAG TGATTGAAGA GAAACCTGAA 
AATAAAACTG GATGGGACAT TGGTGGTGAT GTCTCGAAAA GCACTACCGA TGGAATAGAG 
ACTATTACAT TCCCAAAGCA AGCCCGTGTT GGTAATGATG ATATCTCTCC ACTTCCTTAC 
AACTTGCTCA CCCAACAGCA TATTTCGGAT AAATCAATCG CTGACGCAGT CGAAGCTTTG 
ATCGGAGTAC ATTTGCTGAC GTTGGGACCT AATCCAACGC TGAAGGTGAT GAATTGGATG 
GGACTGAAAG TAATTCAAAA AGATCAGAAG AGTGACGTAC CTTCACCTCT TCTACGATTC 
ATTGACACTC CGACCAATCC GAATGCTTCT CTAAATTTCT TGAACAATTT GTGGCAGCAA 
TTCCAGTTTA CACAATTGGA AGAGAAAATT GGATATCGCT TCAAGGAACG TGCTTATCTT 
GTTCAAGCTT TCACACATGC TTCGTACATC AATAATCGTG TCACAGGATG CTATCAAAGA 
TTAGAATTCC TCGGAGACGC TGTTCTCGAC TATATGATCA CCAGATATTT GTTTGAAGAT 
TCTCGTCAAT ACAGTCCGGG TGTTCTCACT GATTTACGAT CAGCTCTCGT CAATAATACA 
ATTTTCGCGT CGTTAGCAGT GAAATTCGAG TTTCAAAAGC ACTTCATCGC TATGTGCCCA 
GGGCTTTATC ACATGATTGA GAAATTTGTT AAGCTGTGCT CGGAGCGCAA TTTCGATACT 
AATTTCAATG CTGAGATGTA TATGGTGACT ACTGAGGAAG AAATAGATGA AGGCCAAGAA 
GAGGATATTG AAGTTCCAAA AGCGATGGGT GACATATTTG AATCAGTAGC TGGCGCCATT 
TATCTCGACT CTGGACGTAA TTTGGATACC ACTTGGCAAG TGATTTTCCA CATGATGCGC 
GGGACTATTG AATTGTGCTG TGCGAACCCA CCAAGATCAC CGATTCGAGA GCTTATGGAG 
TTCGAGCAGA GCAAAGTTCG ATTCAGTAAA ATGGAACGAA TTCTCGAGAG TGGAAAAGTT 
CGAGTTACAG TCGAGGTTGT TAATAACATG AGATTCACTG GAATGGGAAG GAACTATCGA 
ATCGCGAAAG CTACTGCTGC GAAAAGAGCT TTGAAATACC TCCATCAAAT TGAGCAGCAA 
AGAAGACAAA GCCCATCATT AACAACTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498761.2
CDS	8..5740
Translation	MVRVRADLQCFNPRDYQVELLDKATKKNTIVQLGTGSGKTFIAVLLLKEYGVQLFAPLDQGGKRAFFVVEKVNLVEQQAIHIEVHTSFKVGQVHGQTSSGLWDSKEQCDQFMKRHHVVVITAQCLLDLIRHAYLKIEDMCVLIFDECHHALGSQHPYRSIMVDYKLLKKDKPVPRVLGLTASLIKAKVAPEKLMEQLKKLESAMDSVIETASDLVSLSKYGAKPYEVVIICKDFEIGCLGIPNFDTVIEIFDETVAFVNTTTEFHPDLDLDPRRPIKDSLKTTRAVFRQLGPWAAWRTAQVWEKELGKIIKSQVLPDKTLRFLNMAKTSMITIKRLLEPEMKKIKSIEALRPYVPQRVIRLFEILETFNPEFQKERMKLEKAEHLSAIIFVDQRYIAYSLLLMMRHIKSWEPKFKFVNPDYVVGASGRNLASSDSQGLHKRQTEVLRRFHRNEINCLIATSVLEEGVDVKQCNLVIKFDRPLDMRSYVQSKGRARRAGSRYVITVEEKDTAACDSDLKDFQQIEKILLSRHRTVNNPIEDDSDRFEEFDVDSQMEPYVVEKTGATLKMSTAIALINRYCSKLPSDIFTRLVPHNQIIPIEENGVTKYCAELLLPINSPIKHAIVLKNPMPNKKTAQMAVALEACRQLHLEGELDDNLLPKGRESIAKLLEHIDEEPDEYAPGIAAKVGSSKRKQLYDKKIARALNESFVEADKECFIYAFELERFREAELTLNPKRRKFEDPFNYEYCFGFLSAKEIPKIPPFPVFLRQGNMKVRLIVAPKKTTVTAAQLQEIQLFHNYLFTQVLQMCKTGNLEFDGTSNAPLNTLIVPLNKRKDDMSYTINMKYVSEVVANMENMPRIPKDEVRRQYKFNAEDYKDAIVMPWYRNLEQPVFYYVAEILPEWRPSSKFPDTHFETFNEYFIKKYKLEIYDQNQSLLDVDFTSTRLNLLQPRIQNQPRRSRTVSNSSTSNIPQASASDSKESNTSVPHSSQRQILVPELMDIHPISATLWNVIAALPSIFYRVNQLLLTDELRETILVKAFGKEKTKLDDNVEWNSLAYATEYEEKQTIIVKKIQQLRDLNQKSIEDQERETRENDKIDDGEELFNIGVWDPEEAVRIGVEISSRDDRMDGEDQDTVGLTQGLHDGNISDEDDELPFVMHDYTARLTSNRNGIGAWSGSESIVPSGWGDWDGPEPDNSPMPFQILGGPGGLNVQALMADVGRVFDPSTASSSLSQTVQESTVSPPKQLTKEEEQFKKLQNDLLKQAKERLEALEMSEDMEKPRRLEDTVNLEDYGDDQENQEDENTPTNFPKTIDEEIEELSIGARKKQEIDDNAAKTDVLERENCEVLPVAINEKSRSFSFEKESKAINGRLIRQRSEEYVSHIDSDIGLGVSPCLLLTALTTSNAADGMSLERFETIGDSFLKFATTDYLYHTLLDQHEGKLSFARSKEVSNCNLYRLGKKLGIPQLIVANKFDAHDSWLPPCYIPTCDFKAPNTDDAEEKDNEIERILDGQVIEEKPENKTGWDIGGDVSKSTTDGIETITFPKQARVGNDDISPLPYNLLTQQHISDKSIADAVEALIGVHLLTLGPNPTLKVMNWMGLKVIQKDQKSDVPSPLLRFIDTPTNPNASLNFLNNLWQQFQFTQLEEKIGYRFKERAYLVQAFTHASYINNRVTGCYQRLEFLGDAVLDYMITRYLFEDSRQYSPGVLTDLRSALVNNTIFASLAVKFEFQKHFIAMCPGLYHMIEKFVKLCSERNFDTNFNAEMYMVTTEEEIDEGQEEDIEVPKAMGDIFESVAGAIYLDSGRNLDTTWQVIFHMMRGTIELCCANPPRSPIRELMEFEQSKVRFSKMERILESGKVRVTVEVVNNMRFTGMGRNYRIAKATAAKRALKYLHQIEQQRRQSPSLTTV

Target ORF information:

RefSeq Version	NM_066360.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Death-promoting deoxyribonuclease (dcr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066360.4

ORF Insert Sequence:

ATGGTCAGGG TAAGAGCTGA TTTACAATGT TTTAACCCCA GGGACTACCA GGTGGAACTT 
TTGGATAAAG CGACAAAAAA GAACACAATC GTCCAACTAG GCACTGGTTC AGGAAAAACT 
TTCATTGCAG TTCTACTGCT GAAAGAATAT GGAGTTCAAT TGTTTGCACC ATTGGATCAG 
GGTGGAAAAC GAGCGTTTTT TGTTGTAGAG AAAGTAAACC TCGTTGAACA ACAAGCTATC 
CACATCGAAG TTCACACAAG TTTCAAAGTT GGTCAGGTCC ACGGACAGAC AAGTAGTGGT 
TTATGGGACA GCAAAGAACA ATGTGATCAG TTCATGAAGA GGCACCATGT TGTTGTGATC 
ACAGCTCAAT GCCTTCTTGA TTTGATTCGA CATGCGTACT TGAAAATTGA AGACATGTGT 
GTTCTGATCT TTGACGAATG TCATCATGCT CTCGGTTCTC AACATCCATA CAGAAGCATC 
ATGGTCGATT ATAAGTTATT GAAAAAGGAC AAACCCGTCC CACGTGTATT AGGACTCACA 
GCCAGTCTTA TCAAAGCGAA AGTTGCGCCG GAAAAACTAA TGGAACAGTT AAAAAAGTTG 
GAATCTGCGA TGGACAGTGT TATAGAGACA GCTAGTGATT TAGTCTCGCT CTCGAAATAC 
GGTGCGAAAC CCTATGAAGT TGTGATCATC TGCAAAGACT TTGAAATTGG GTGCCTGGGT 
ATTCCGAATT TCGACACAGT TATAGAAATA TTTGATGAAA CTGTAGCCTT TGTGAACACC 
ACAACAGAAT TTCATCCAGA TCTAGATTTG GATCCTCGTA GACCGATTAA AGATTCTCTC 
AAGACGACGC GAGCTGTTTT TCGTCAACTG GGACCTTGGG CGGCATGGCG AACGGCTCAA 
GTTTGGGAGA AAGAGTTGGG AAAAATTATA AAATCTCAAG TGCTACCGGA TAAGACGTTG 
CGGTTTTTGA ATATGGCAAA AACATCTATG ATTACGATTA AAAGATTGTT GGAACCGGAG 
ATGAAGAAAA TCAAAAGTAT TGAAGCTCTC CGTCCATATG TTCCTCAACG TGTCATACGT 
CTTTTCGAAA TCCTAGAAAC TTTCAACCCC GAATTTCAAA AAGAACGAAT GAAGTTAGAA 
AAAGCAGAAC ATTTATCAGC CATTATTTTT GTCGATCAAC GGTACATTGC ATATTCGTTA 
CTTCTTATGA TGCGTCATAT TAAATCATGG GAGCCTAAGT TCAAGTTTGT GAACCCGGAT 
TACGTCGTTG GAGCCAGCGG ACGAAATTTG GCGAGTTCGG ATAGTCAAGG ATTACATAAA 
CGACAAACTG AAGTTCTTCG CCGTTTCCAT CGAAATGAAA TCAATTGTCT TATCGCTACA 
TCAGTGCTTG AAGAAGGTGT AGATGTTAAA CAATGTAATC TTGTGATTAA ATTTGATCGA 
CCACTTGACA TGAGATCATA CGTTCAGTCA AAGGGAAGAG CTCGGCGAGC TGGATCAAGA 
TATGTTATCA CTGTTGAGGA GAAAGATACT GCGGCGTGTG ATAGTGATTT GAAAGATTTC 
CAGCAAATAG AAAAAATTCT TCTTAGCCGC CATCGTACAG TCAACAATCC TATTGAAGAT 
GATTCGGATA GATTTGAAGA ATTTGATGTT GATTCTCAAA TGGAACCATA TGTTGTTGAA 
AAAACTGGTG CAACATTGAA AATGTCAACT GCTATCGCAT TAATTAATAG ATATTGTTCG 
AAGCTTCCTT CCGATATCTT CACTCGTCTT GTTCCACACA ATCAAATCAT TCCAATTGAA 
GAGAATGGAG TGACCAAATA CTGTGCAGAG CTTCTTCTTC CAATTAACTC ACCGATCAAA 
CATGCAATTG TGCTCAAAAA TCCAATGCCA AATAAGAAGA CTGCACAGAT GGCAGTTGCA 
CTTGAAGCAT GTCGACAATT ACATTTAGAA GGAGAACTCG ATGATAATTT ACTTCCAAAA 
GGAAGAGAGT CGATTGCCAA ATTATTAGAA CATATTGATG AGGAGCCGGA TGAGTATGCA 
CCTGGAATTG CTGCTAAAGT TGGATCATCA AAACGAAAGC AATTATATGA CAAAAAGATT 
GCTCGTGCTC TCAATGAATC CTTCGTGGAA GCAGATAAGG AATGTTTCAT CTATGCATTC 
GAATTGGAAC GTTTCCGGGA AGCCGAACTT ACGTTGAACC CGAAAAGACG AAAGTTTGAA 
GATCCATTTA ATTATGAATA TTGCTTCGGA TTTCTGAGCG CAAAAGAGAT ACCAAAGATC 
CCACCATTCC CAGTGTTTCT TCGACAAGGA AATATGAAAG TCCGATTGAT TGTCGCACCG 
AAAAAGACTA CCGTAACCGC TGCTCAGCTT CAAGAGATTC AACTGTTCCA TAATTATTTG 
TTCACACAAG TTCTTCAAAT GTGTAAAACG GGAAATTTGG AATTCGATGG AACATCAAAT 
GCACCATTGA ACACGTTGAT TGTTCCACTT AACAAGCGAA AAGATGATAT GTCCTACACA 
ATCAACATGA AATATGTATC GGAAGTAGTT GCAAACATGG AAAATATGCC ACGTATTCCA 
AAAGATGAAG TTCGTCGTCA ATACAAATTC AACGCTGAAG ATTATAAGGA TGCGATCGTA 
ATGCCGTGGT ATCGAAATCT GGAACAGCCT GTATTCTACT ATGTTGCTGA AATTCTACCA 
GAATGGAGGC CGTCGTCGAA ATTCCCGGAT ACGCACTTCG AAACGTTTAA CGAGTATTTC 
ATCAAGAAAT ATAAATTAGA GATTTATGAT CAGAATCAAA GTTTATTGGA TGTAGATTTC 
ACATCTACTC GTCTTAACCT TCTTCAACCT CGAATTCAAA ATCAACCACG TCGTTCAAGA 
ACTGTGAGTA ACTCCTCAAC GTCAAATATT CCTCAAGCAT CTGCTTCTGA TTCAAAAGAG 
TCGAATACTT CCGTTCCACA CTCTTCACAA CGTCAGATCC TTGTACCGGA ATTGATGGAC 
ATTCATCCTA TCTCTGCGAC TCTTTGGAAT GTTATAGCAG CCCTGCCAAG TATTTTCTAT 
CGTGTTAATC AGTTATTGTT GACTGATGAA CTTCGAGAAA CAATTTTGGT GAAAGCTTTT 
GGAAAAGAGA AAACTAAACT GGATGATAAT GTTGAATGGA ATTCTCTGGC ATATGCAACC 
GAATATGAAG AGAAACAAAC GATAATCGTT AAGAAAATTC AACAATTGAG AGATTTAAAT 
CAAAAATCGA TTGAAGATCA AGAAAGAGAG ACGAGAGAAA ATGATAAGAT TGACGATGGT 
GAGGAGTTAT TCAATATTGG AGTTTGGGAT CCAGAAGAGG CAGTCAGAAT TGGTGTAGAA 
ATTAGTTCAA GAGATGATAG AATGGACGGT GAAGATCAGG ATACTGTAGG ACTAACACAA 
GGACTTCATG ATGGGAATAT ATCCGATGAA GACGATGAAC TGCCGTTTGT AATGCATGAT 
TACACAGCCA GGCTCACTTC TAACAGAAAT GGAATTGGTG CATGGTCAGG ATCTGAGTCT 
ATTGTTCCAA GCGGATGGGG AGATTGGGAT GGACCTGAAC CAGATAATAG TCCCATGCCT 
TTCCAAATTC TCGGTGGACC AGGTGGTTTG AATGTGCAGG CACTGATGGC TGATGTCGGA 
AGAGTTTTTG ATCCATCTAC TGCTTCGTCT TCCCTTTCTC AAACTGTACA AGAATCGACA 
GTTTCACCTC CAAAACAATT GACAAAAGAA GAAGAACAGT TTAAGAAACT TCAAAATGAC 
TTGCTGAAAC AAGCAAAAGA GCGACTTGAA GCTCTAGAAA TGTCAGAAGA TATGGAGAAA 
CCGCGAAGAT TGGAGGATAC TGTGAATTTA GAAGATTATG GAGATGATCA AGAAAATCAA 
GAGGATGAGA ATACACCAAC AAATTTCCCG AAAACCATAG ATGAAGAAAT TGAAGAGTTG 
TCAATTGGAG CCCGGAAAAA ACAAGAAATA GACGACAATG CAGCTAAAAC TGATGTACTC 
GAAAGGGAAA ACTGTGAAGT TTTGCCAGTT GCTATAAATG AAAAGTCTCG TTCATTCTCT 
TTTGAAAAAG AAAGTAAAGC AATAAATGGA CGACTTATAA GACAACGATC AGAGGAATAC 
GTGTCACATA TTGATTCAGA TATTGGATTA GGAGTATCTC CATGTCTTTT GTTAACTGCT 
CTTACTACTT CCAATGCTGC AGATGGAATG TCATTAGAGA GATTCGAAAC AATCGGAGAC 
TCTTTCTTGA AGTTTGCTAC TACCGATTAT CTCTATCACA CGTTGCTTGA TCAACATGAA 
GGAAAATTGT CGTTTGCACG TAGTAAAGAA GTTTCAAATT GCAATTTGTA TCGACTGGGC 
AAGAAATTGG GAATACCTCA ATTGATTGTT GCAAATAAAT TCGATGCTCA CGATTCTTGG 
CTGCCACCAT GCTATATTCC AACTTGCGAC TTCAAAGCTC CTAACACTGA TGATGCAGAA 
GAGAAAGATA ATGAAATTGA ACGAATTTTG GATGGACAAG TGATTGAAGA GAAACCTGAA 
AATAAAACTG GATGGGACAT TGGTGGTGAT GTCTCGAAAA GCACTACCGA TGGAATAGAG 
ACTATTACAT TCCCAAAGCA AGCCCGTGTT GGTAATGATG ATATCTCTCC ACTTCCTTAC 
AACTTGCTCA CCCAACAGCA TATTTCGGAT AAATCAATCG CTGACGCAGT CGAAGCTTTG 
ATCGGAGTAC ATTTGCTGAC GTTGGGACCT AATCCAACGC TGAAGGTGAT GAATTGGATG 
GGACTGAAAG TAATTCAAAA AGATCAGAAG AGTGACGTAC CTTCACCTCT TCTACGATTC 
ATTGACACTC CGACCAATCC GAATGCTTCT CTAAATTTCT TGAACAATTT GTGGCAGCAA 
TTCCAGTTTA CACAATTGGA AGAGAAAATT GGATATCGCT TCAAGGAACG TGCTTATCTT 
GTTCAAGCTT TCACACATGC TTCGTACATC AATAATCGTG TCACAGGATG CTATCAAAGA 
TTAGAATTCC TCGGAGACGC TGTTCTCGAC TATATGATCA CCAGATATTT GTTTGAAGAT 
TCTCGTCAAT ACAGTCCGGG TGTTCTCACT GATTTACGAT CAGCTCTCGT CAATAATACA 
ATTTTCGCGT CGTTAGCAGT GAAATTCGAG TTTCAAAAGC ACTTCATCGC TATGTGCCCA 
GGGCTTTATC ACATGATTGA GAAATTTGTT AAGCTGTGCT CGGAGCGCAA TTTCGATACT 
AATTTCAATG CTGAGATGTA TATGGTGACT ACTGAGGAAG AAATAGATGA AGGCCAAGAA 
GAGGATATTG AAGTTCCAAA AGCGATGGGT GACATATTTG AATCAGTAGC TGGCGCCATT 
TATCTCGACT CTGGACGTAA TTTGGATACC ACTTGGCAAG TGATTTTCCA CATGATGCGC 
GGGACTATTG AATTGTGCTG TGCGAACCCA CCAAGATCAC CGATTCGAGA GCTTATGGAG 
TTCGAGCAGA GCAAAGTTCG ATTCAGTAAA ATGGAACGAA TTCTCGAGAG TGGAAAAGTT 
CGAGTTACAG TCGAGGTTGT TAATAACATG AGATTCACTG GAATGGGAAG GAACTATCGA 
ATCGCGAAAG CTACTGCTGC GAAAAGAGCT TTGAAATACC TCCATCAAAT TGAGCAGCAA 
AGAAGACAAA GCCCATCATT AACAACTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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dcr-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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