dicer1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dicer1
Entrez Gene ID	100170154
Full Name	dicer 1, ribonuclease III
Synonyms	dcr1,dicer,herna
General protein information	Preferred Names dicer 1, ribonuclease III Names endoribonuclease Dicer dicer 1, ribonuclease type III
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031890235.1	XP_031746095.1	endoribonuclease Dicer isoform X1
XM_012968074.2	XP_012823528.1	endoribonuclease Dicer isoform X1
NM_001129918.2	NP_001123390.2	endoribonuclease Dicer
XM_031890234.1	XP_031746094.1	endoribonuclease Dicer isoform X1
XM_012968075.2	XP_012823529.1	endoribonuclease Dicer isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DICER1	NP_085124.2
Rattus norvegicus (Norway rat)	Dicer1	XP_006240573.1
Danio rerio (zebrafish)	dicer1	NP_001154925.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002836	XP_003436256.1
Magnaporthe oryzae (rice blast fungus)	MGG_01541	XP_003714515.1
Neurospora crassa	NCU08270	XP_961898.1
	DICER1	XP_001100868.1
Gallus gallus (chicken)	DICER1	NP_001035555.1
Drosophila melanogaster (fruit fly)	Dcr-1	NP_524453.1
Caenorhabditis elegans (roundworm)	dcr-1	NP_498761.2
Xenopus tropicalis (tropical clawed frog)	dicer1	NP_001123390.2
Canis lupus familiaris (dog)	DICER1	XP_868526.3
Bos taurus (cattle)	DICER1	NP_976235.1
Schizosaccharomyces pombe (fission yeast)	dcr1	NP_588215.2
Pan troglodytes (chimpanzee)	DICER1	XP_001154369.1
Mus musculus (house mouse)	Dicer1	NP_683750.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dicer1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dicer1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa63519	XM_031890234.1 Latest version!	Xenopus tropicalis dicer 1, ribonuclease III (dicer1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa63519	XM_031890235.1 Latest version!	Xenopus tropicalis dicer 1, ribonuclease III (dicer1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa25838	NM_001129918.2 Latest version!	Xenopus tropicalis dicer 1, ribonuclease III (dicer1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32251	XM_012968074.2 Latest version!	Xenopus tropicalis dicer 1, ribonuclease type III (dicer1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32251	XM_012968075.2 Latest version!	Xenopus tropicalis dicer 1, ribonuclease type III (dicer1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dicer1 ( XM_031890235.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746095.1 Xenopus tropicalis dicer 1, ribonuclease III (dicer1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa63519
Clone ID Related Accession (Same CDS sequence)	XM_031890235.1 , XM_031890234.1
Accession Version	XM_031890235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCC TTCAGCTCAT GACTCCGGCC TCCTCTCCAA TGGGTCCCTT CTTTGGGCTA 
CCCTGGCAAC AGGAGGCTAT TCATGACAAC ATTTATACCC CACGGAAATA TCAGGTGGAA 
CTACTCGAAG CAGCATTGGA TCATAATACT ATAGTATGTT TGAATTCTGG CTCAGGGAAG 
ACATTTATTG CAGTACTACT CAGTAAAGAG CTGTCATACC AGATCCGTGG GGACTTCAGC 
AAAAATACTA AGAGGACTGT GTTTTTGGTC AATTCCGAGA AACAGGTTTC TCAACAAGTG 
TCTGCTGTCA GGACCCATAC AGACCTCAAG GTGGGAGAAT ATTCAGATCA AGAAAAAACA 
CAATGCTGGG CAAAAGAAAG ATGGTACCTA GAATTTGAAA CTCATCAGGT CTTGGTCATG 
ACCTGCCACA TCTTCCTGAA CGTTCTGAAA AGTGGGAACG TGTCATTGTC AAACATTAAT 
CTGTTAGTGT TTGATGAATG TCATCTTGCA ATTCAGGATC ACCCATATCG AGAAATCATG 
AAGATATGTG AGAGTTGCCA GCCATGCCCT CGAATCCTGG GTCTAACTGC TTCAATTTTA 
AATGGAAAAT GTGACCCTCG TGACCTAGAG GAAAAGATCC AGAAACTGGA GGAAATATTA 
AGGAGTAATG CAGAAACTGC AACTGATTTG GTTGTTTTAG ACAGGTATGC TTCCCAACCA 
TGTGAAATTG TATTGGACTG TGGGCCATAT ATTGACAAAA GTGGACTTTA TCAAAGACTT 
CTAAATGAAT TGGATGAAGC CCTTAACTTT CTCATTGACT GTAATATTTC TACACATTCT 
AAAGAGAGAG ATTCCACATT AATTTCAAAA CAGATTCTAT CAGACTGTCA AACTGTTCTT 
TTGGTCTTGG GACCATGGTG TGCTGATAAA GTTGCGGGGA TGATGGTGAG AGAGCTGCAG 
AAGTATATCA AACATGAGCA GGAGGAACTG CACAGAAAAT TCCTTTTGTT TACAGACACT 
ATCTTAAGGA AGATCCATGC TCTTTGTGAG GAACACTTCT CGCCAGCCTC TCTTGATATG 
AAGTTTGTCA CACCTAAAGT TATAAAACTG CTGGAAATTT TACGCAAATA CAAACCCTAC 
GAACGCCAGC AATTTGAAAG TGTTGAATGG TATAACAATA GAAACCAGGA TAATTATGTG 
TCTTGGAGTG ATTCAGAGGA TGATGACGAT GAAGATGAAG AAATTGAGGA GAAAGAGAAA 
ACTGAAACAA GCTTTCCATC CCCATTCACA AACATCCTGT GTGGCATCAT CTTCGTGGAA 
CGAAGATACA CAGCAGTAGT ATTAAACAGG TTGATTAAAG AAGCCGGGAA GCAAGATCCA 
GAGCTGGCCT ATATCAGTAG TAACTTTATT ACTGGGCACG GCATAGGAAA GAACCAGCCA 
CGCAATAAGC AGATGGAAGT TGAGTTTAGA AAGCAAGAAG AGGTGCTTCG TAAATTTCGT 
GCACACGAAA CCAACTTATT GATAGCTACT AGCATTGTTG AGGAAGGAGT GGACATACCA 
AAATGCAACT TGGTAGTTCG ATTTGATTTA CCTTCAGAGT ACAGATCCTA TGTACAGTCC 
AAAGGCAGAG CAAGAGCACC AATCTCAAAT TACATCATGC TAGCCGATAG TGATAAAATT 
AAGGCATTTG AAGAGGACCT TAAAACATAC AAAGCAATTG AAAAGATTCT GCGGAACAAA 
TGCTCAAAGT CCATTGATTG TGGAAATACA GAATCTGAGC CCATTGTGGA CGATGATGAA 
ATATTTCCAC CATATGTGTT GAGACAGGAT GATGGCAGCC CACGAGTTAC TATCAACACC 
GCTATTGGAC ACATTAACAG GTACTGTGCT AGGCTACCTA GTGACCCATT TACTCATCTT 
GCTCCTAAGT GCAAAACACG AGAGTTCCCT GATGGACTTT ATCGCTCAAC ACTCTACCTG 
CCAATTAATT CTCCACTTAG AGCCCCCATT GTTGGCCCTC CGATGAATTG TGGAAGGCTA 
GCTGATAGAG CTGTAGCTCT TATATGCTGT AAAAAACTAC ATGAAATTGG TGAACTGGAT 
GATCATTTAA TGCCAGTTGG CAAGGAAACT GTAAAATATG AGGAGGAGCT TGATTTGCAT 
GATGAGGAAG AAACCAGTGT TCCAGGCAGA CCAGGATCCA CAAAAAGAAG GCAGTGTTAT 
CCAAAAGCTA TTCCTGAATG TTTACGGAAC AGCTACCCCA AGCCTGGTCA GCCTTGTTAC 
TTATATGTAA TAGGAATGGT ATTAACCACT CCTCTACCAG ATGAACTTAA TTTTAGGCGA 
CGGAAGCTGT ATCCCCCTGA AGACACAACA AGATGCTTTG GAATACTAAC TGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTCTAT ACTCGTTCGG GAGAGGTGAC CATATCAATT 
GAACTAAAGA AGTCTGGTTT TACATTAAAC TTAGAACAAC TTGAGCTCAT TACTAGACTC 
CACCAGTACA TTTTTTCACA CATTCTTCGT CTTGAGAAAC CTGCACTAGA ATTTAAACCC 
ACAGTAGCCG ATTGTGCCTA CTGTGTTCTA CCTCTTAACG TTGTAAATGA TTCTGGCACT 
TTGGACATTG ACTTCAAATT TGTAGAAGAT ATTGAGAAAT CAGAAGCACG TACTGGTATA 
CCTAATACAC AGTATTCAGC TGAAAGTCCT TTTATTTTCA AATTAGAAGA CTACCAGGAT 
GCTGTTATCA TTCCAAGATA TCGAAATTTT GATCAGCCGC ACAGATTTTA TGTAGCTGAT 
GTATACACTG ATCTTACACC GCTCAGCAAG TTCCCTTCCC CTGAATATGA AACCTTTGCT 
GAATATTACA AAACAAAGTA TAACCTCGAT CTCACAAACC TCAATCAGCC ACTTTTGGAT 
GTGGACCACA CATCATCAAG ACTTAATTTG CTGACTCCTC GCCATCTGAA TCAGAAAGGT 
AAAGCTCTGC CATTAAGCAG TGCTGAAAAG AGAAAAGCCA AATGGGAGAG TTTACAAAAC 
AAACAGATCC TGGTTCCAGA GCTTTGTGCT ATACATCCAG TCCCAGCATC CTTATGGAGA 
AAAGCTGTTT GTTTGCCAAG CATACTTTAT CGATTACATT GCCTCCTTAC AGCAGAAGAG 
CTGAGAGCGC AAACAGCTAT TGATGCTGGG GTAGGAGTCA AATCGCTTCC TGATGATTTC 
CGATACCCAA ACTTGGATTT TGGATGGAAA CGATCTATAG ACAGTAAAAC ATTCATCTCT 
AATCAAAGTT CCTCATCAGT CGAGAGTGAA AGTGACTGCA GACTCAATAA AACCACGGCC 
CCTGACAGTG CTGCAAGCTC AGCTGCTAAT TCTGTAATCT ACATGCAAAT CAATGACCAA 
ATGTCTGTGA ACTGCACACC ACCATGTCAA AAATCTCTGA GTCACCTCCA AACAGTTTGC 
TTCTCAGATG ATTACAAAGC AATTAACGGT ATTTCTTGCA ATGGTCTTAC CAATGGTGAT 
TGGGAAGCAG AAAGCGCTGC ATGTTTCCAA AAGGACGAAC GGATAACCTG CAAACAGGAA 
ATACCTGAAA AATCTACCTC GTTTCACGTT CAGAATTTAC CTAAGGAGAA CCAGCCCATA 
CTTAAAGAAT GTACTCTGAG CAACTCTGAT GGAAATGTCA GCAAACCTAC CTCAGATGAG 
TGCCCTAGCA CCTGTACTTC AGACATGCAT TATGATAGTG GGCTTTCCAA TAGGCACTCT 
TCAAAAACTC TTGGCCCAAA CCCTGGTCTT ATCCTTCAGG CCTTGACTCT GTCCAATGCA 
AGTGATGGTT TCAATCTGGA GCGTCTTGAA ATGCTTGGGG ACTCTTTCTT AAAACATGCT 
ATCACCACAT ATCTGTTCTG CACATACCCA GATGCTCACG AGGGCCGTCT ATCCTATATG 
CGCAGCAAGA AGGTCAGTAA CTGTAACCTT TATCGGCTTG GAAAAAAGAA AGGCTCACCC 
AGCCGGATGG TGGTGTCCAT TTTCGATCCA CCTGTCAACT GGCTTCCCCC TGGTTATATT 
GTTAACCAGG ACAAAAACTC TGATAAGTGG GAAAGTAATG AAACGTCTGG TGAGGATGTG 
ATGGTTAATG GCAAAATTGA TGAAGACTTT GATGATGAAG AAGATGAAGA TCTTATGTGG 
AGGAATCCCA AAGAGGAAAC TGATTTTGAT GATGACTTTT TGGAATATGA TCAAGAGCAT 
ATAAAATTCA TTGACAGCAT GTTAATGGGA TCTGGGGCAT TTGTGAAGAA AATTCCTCTT 
TCTTCATTTG CACCCCCTGA TCAGAACTAT GAATGGAGGG CGCCAAAGAA ACCTCCACTG 
GAAAGTTCTC AATTTCCCTG CGATTTTGAT GATTTTGATT ACAGTTCTTG GGATGCCATG 
TGTTATTTGG ATCCCAGCAA GGCTGTAGAG GAGGATGACT TTGTAGTAGG CTTTTGGAAT 
CCATCAGAAG AGAACTGTGG AGCTGATGCT GGAAAGCAGT CTATATCTTA TGACCTACAC 
ACAGAGCAGT GTATTGCAGA CAAAAGTATA GCTGACTGTG TAGAGGCTTT ATTAGGCTGC 
TATCTGACCA GTTGTGGTGA GAGAGCTGCA CAACTTTTTC TTTGTTCTTT GGGCTTAAAA 
GTGCTCCCAG AAGTAAGAAA GCTTGTTACA AACACAAATG TCATTAGTGC ATCATCATCT 
TACCAGAACA GTACCAGAGA CAACTGCACT CTTACTGCCA GAACCAACAC TGATCTGTCC 
TCCTGCAAAG GCATAGACTA CGGTTATTTA AAGATTCCCC CAAGGTGTAT GTTTGAGCAC 
CCAGATGCTG AGAAAACCCT TGACCATCTA ATATCTGGTT TTGAAAACTT TGAGAAGAAA 
ATAAACTATC CATTTAAAAA CAAGGCTTAC CTTCTACAGG CTTTTACACA TGCCTCTTAC 
CACTACAATA CTATAACTGA CTGCTACCAG CGTTTAGAAT TTCTTGGAGA TGCAATTTTG 
GACTACCTCA TTACTAAGCA CCTTTATGAA GATCCACGCC AGCACTCGCC AGGAGTCCTC 
ACTGACCTGC GCTCTGCTCT TGTGAATAAC ACCATATTTG CATCACTAGC TGTGAAATAT 
GACTACCATA AATACTTCAA GGCTATCTCT CCTGAACTGT TCCATGTAAT TGATGATTTT 
GTGCAATTTC AACTTGAAAA AAATGAAATG CAAGGCATGG ATTCTGAGTT ACGTAGGTCT 
GAAGAAGACG AAGAGAAAGA AGAGGACATT GAAGTCCCAA AAGCTATGGG AGACATATTT 
GAGTCACTTG CAGGAGCTAT TTACATGGAC AGCGGCATGT CTTTGGAAAC TGTATGGCAT 
GTGTATTATC CTATGATGCA GCCATTAATA GAAAAATTCT CTGCTAATGT TCCTCGTTCC 
CCAGTGAGAG AATTACTGGA AATGGAGCCT GAAACTGCTA AATTCAGTCC AGCCGAAAGA 
ACATACGATG GAAAAGTCAG AGTGACTGTG GAAGTTGTTG GAAAAGGAAA ATTTAAAGGA 
GTCGGAAGAA GTTACAGAAT TGCCAAATCT GCAGCAGCCA GGAGAGCACT AAGAAGCCTC 
AAAGCAAATC AATCTCAGGT CCCTAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746095.1
CDS	276..5948
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNSGSGKTFIAVLLSKELSYQIRGDFSKNTKRTVFLVNSEKQVSQQVSAVRTHTDLKVGEYSDQEKTQCWAKERWYLEFETHQVLVMTCHIFLNVLKSGNVSLSNINLLVFDECHLAIQDHPYREIMKICESCQPCPRILGLTASILNGKCDPRDLEEKIQKLEEILRSNAETATDLVVLDRYASQPCEIVLDCGPYIDKSGLYQRLLNELDEALNFLIDCNISTHSKERDSTLISKQILSDCQTVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTILRKIHALCEEHFSPASLDMKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDEDEEIEEKEKTETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPSEYRSYVQSKGRARAPISNYIMLADSDKIKAFEEDLKTYKAIEKILRNKCSKSIDCGNTESEPIVDDDEIFPPYVLRQDDGSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTREFPDGLYRSTLYLPINSPLRAPIVGPPMNCGRLADRAVALICCKKLHEIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLNLEQLELITRLHQYIFSHILRLEKPALEFKPTVADCAYCVLPLNVVNDSGTLDIDFKFVEDIEKSEARTGIPNTQYSAESPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPVPASLWRKAVCLPSILYRLHCLLTAEELRAQTAIDAGVGVKSLPDDFRYPNLDFGWKRSIDSKTFISNQSSSSVESESDCRLNKTTAPDSAASSAANSVIYMQINDQMSVNCTPPCQKSLSHLQTVCFSDDYKAINGISCNGLTNGDWEAESAACFQKDERITCKQEIPEKSTSFHVQNLPKENQPILKECTLSNSDGNVSKPTSDECPSTCTSDMHYDSGLSNRHSSKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGSPSRMVVSIFDPPVNWLPPGYIVNQDKNSDKWESNETSGEDVMVNGKIDEDFDDEEDEDLMWRNPKEETDFDDDFLEYDQEHIKFIDSMLMGSGAFVKKIPLSSFAPPDQNYEWRAPKKPPLESSQFPCDFDDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGADAGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPEVRKLVTNTNVISASSSYQNSTRDNCTLTARTNTDLSSCKGIDYGYLKIPPRCMFEHPDAEKTLDHLISGFENFEKKINYPFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWHVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQSQVPNS

Target ORF information:

RefSeq Version	XM_031890235.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis dicer 1, ribonuclease III (dicer1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890235.1

ORF Insert Sequence:

ATGGCAGGCC TTCAGCTCAT GACTCCGGCC TCCTCTCCAA TGGGTCCCTT CTTTGGGCTA 
CCCTGGCAAC AGGAGGCTAT TCATGACAAC ATTTATACCC CACGGAAATA TCAGGTGGAA 
CTACTCGAAG CAGCATTGGA TCATAATACT ATAGTATGTT TGAATTCTGG CTCAGGGAAG 
ACATTTATTG CAGTACTACT CAGTAAAGAG CTGTCATACC AGATCCGTGG GGACTTCAGC 
AAAAATACTA AGAGGACTGT GTTTTTGGTC AATTCCGAGA AACAGGTTTC TCAACAAGTG 
TCTGCTGTCA GGACCCATAC AGACCTCAAG GTGGGAGAAT ATTCAGATCA AGAAAAAACA 
CAATGCTGGG CAAAAGAAAG ATGGTACCTA GAATTTGAAA CTCATCAGGT CTTGGTCATG 
ACCTGCCACA TCTTCCTGAA CGTTCTGAAA AGTGGGAACG TGTCATTGTC AAACATTAAT 
CTGTTAGTGT TTGATGAATG TCATCTTGCA ATTCAGGATC ACCCATATCG AGAAATCATG 
AAGATATGTG AGAGTTGCCA GCCATGCCCT CGAATCCTGG GTCTAACTGC TTCAATTTTA 
AATGGAAAAT GTGACCCTCG TGACCTAGAG GAAAAGATCC AGAAACTGGA GGAAATATTA 
AGGAGTAATG CAGAAACTGC AACTGATTTG GTTGTTTTAG ACAGGTATGC TTCCCAACCA 
TGTGAAATTG TATTGGACTG TGGGCCATAT ATTGACAAAA GTGGACTTTA TCAAAGACTT 
CTAAATGAAT TGGATGAAGC CCTTAACTTT CTCATTGACT GTAATATTTC TACACATTCT 
AAAGAGAGAG ATTCCACATT AATTTCAAAA CAGATTCTAT CAGACTGTCA AACTGTTCTT 
TTGGTCTTGG GACCATGGTG TGCTGATAAA GTTGCGGGGA TGATGGTGAG AGAGCTGCAG 
AAGTATATCA AACATGAGCA GGAGGAACTG CACAGAAAAT TCCTTTTGTT TACAGACACT 
ATCTTAAGGA AGATCCATGC TCTTTGTGAG GAACACTTCT CGCCAGCCTC TCTTGATATG 
AAGTTTGTCA CACCTAAAGT TATAAAACTG CTGGAAATTT TACGCAAATA CAAACCCTAC 
GAACGCCAGC AATTTGAAAG TGTTGAATGG TATAACAATA GAAACCAGGA TAATTATGTG 
TCTTGGAGTG ATTCAGAGGA TGATGACGAT GAAGATGAAG AAATTGAGGA GAAAGAGAAA 
ACTGAAACAA GCTTTCCATC CCCATTCACA AACATCCTGT GTGGCATCAT CTTCGTGGAA 
CGAAGATACA CAGCAGTAGT ATTAAACAGG TTGATTAAAG AAGCCGGGAA GCAAGATCCA 
GAGCTGGCCT ATATCAGTAG TAACTTTATT ACTGGGCACG GCATAGGAAA GAACCAGCCA 
CGCAATAAGC AGATGGAAGT TGAGTTTAGA AAGCAAGAAG AGGTGCTTCG TAAATTTCGT 
GCACACGAAA CCAACTTATT GATAGCTACT AGCATTGTTG AGGAAGGAGT GGACATACCA 
AAATGCAACT TGGTAGTTCG ATTTGATTTA CCTTCAGAGT ACAGATCCTA TGTACAGTCC 
AAAGGCAGAG CAAGAGCACC AATCTCAAAT TACATCATGC TAGCCGATAG TGATAAAATT 
AAGGCATTTG AAGAGGACCT TAAAACATAC AAAGCAATTG AAAAGATTCT GCGGAACAAA 
TGCTCAAAGT CCATTGATTG TGGAAATACA GAATCTGAGC CCATTGTGGA CGATGATGAA 
ATATTTCCAC CATATGTGTT GAGACAGGAT GATGGCAGCC CACGAGTTAC TATCAACACC 
GCTATTGGAC ACATTAACAG GTACTGTGCT AGGCTACCTA GTGACCCATT TACTCATCTT 
GCTCCTAAGT GCAAAACACG AGAGTTCCCT GATGGACTTT ATCGCTCAAC ACTCTACCTG 
CCAATTAATT CTCCACTTAG AGCCCCCATT GTTGGCCCTC CGATGAATTG TGGAAGGCTA 
GCTGATAGAG CTGTAGCTCT TATATGCTGT AAAAAACTAC ATGAAATTGG TGAACTGGAT 
GATCATTTAA TGCCAGTTGG CAAGGAAACT GTAAAATATG AGGAGGAGCT TGATTTGCAT 
GATGAGGAAG AAACCAGTGT TCCAGGCAGA CCAGGATCCA CAAAAAGAAG GCAGTGTTAT 
CCAAAAGCTA TTCCTGAATG TTTACGGAAC AGCTACCCCA AGCCTGGTCA GCCTTGTTAC 
TTATATGTAA TAGGAATGGT ATTAACCACT CCTCTACCAG ATGAACTTAA TTTTAGGCGA 
CGGAAGCTGT ATCCCCCTGA AGACACAACA AGATGCTTTG GAATACTAAC TGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTCTAT ACTCGTTCGG GAGAGGTGAC CATATCAATT 
GAACTAAAGA AGTCTGGTTT TACATTAAAC TTAGAACAAC TTGAGCTCAT TACTAGACTC 
CACCAGTACA TTTTTTCACA CATTCTTCGT CTTGAGAAAC CTGCACTAGA ATTTAAACCC 
ACAGTAGCCG ATTGTGCCTA CTGTGTTCTA CCTCTTAACG TTGTAAATGA TTCTGGCACT 
TTGGACATTG ACTTCAAATT TGTAGAAGAT ATTGAGAAAT CAGAAGCACG TACTGGTATA 
CCTAATACAC AGTATTCAGC TGAAAGTCCT TTTATTTTCA AATTAGAAGA CTACCAGGAT 
GCTGTTATCA TTCCAAGATA TCGAAATTTT GATCAGCCGC ACAGATTTTA TGTAGCTGAT 
GTATACACTG ATCTTACACC GCTCAGCAAG TTCCCTTCCC CTGAATATGA AACCTTTGCT 
GAATATTACA AAACAAAGTA TAACCTCGAT CTCACAAACC TCAATCAGCC ACTTTTGGAT 
GTGGACCACA CATCATCAAG ACTTAATTTG CTGACTCCTC GCCATCTGAA TCAGAAAGGT 
AAAGCTCTGC CATTAAGCAG TGCTGAAAAG AGAAAAGCCA AATGGGAGAG TTTACAAAAC 
AAACAGATCC TGGTTCCAGA GCTTTGTGCT ATACATCCAG TCCCAGCATC CTTATGGAGA 
AAAGCTGTTT GTTTGCCAAG CATACTTTAT CGATTACATT GCCTCCTTAC AGCAGAAGAG 
CTGAGAGCGC AAACAGCTAT TGATGCTGGG GTAGGAGTCA AATCGCTTCC TGATGATTTC 
CGATACCCAA ACTTGGATTT TGGATGGAAA CGATCTATAG ACAGTAAAAC ATTCATCTCT 
AATCAAAGTT CCTCATCAGT CGAGAGTGAA AGTGACTGCA GACTCAATAA AACCACGGCC 
CCTGACAGTG CTGCAAGCTC AGCTGCTAAT TCTGTAATCT ACATGCAAAT CAATGACCAA 
ATGTCTGTGA ACTGCACACC ACCATGTCAA AAATCTCTGA GTCACCTCCA AACAGTTTGC 
TTCTCAGATG ATTACAAAGC AATTAACGGT ATTTCTTGCA ATGGTCTTAC CAATGGTGAT 
TGGGAAGCAG AAAGCGCTGC ATGTTTCCAA AAGGACGAAC GGATAACCTG CAAACAGGAA 
ATACCTGAAA AATCTACCTC GTTTCACGTT CAGAATTTAC CTAAGGAGAA CCAGCCCATA 
CTTAAAGAAT GTACTCTGAG CAACTCTGAT GGAAATGTCA GCAAACCTAC CTCAGATGAG 
TGCCCTAGCA CCTGTACTTC AGACATGCAT TATGATAGTG GGCTTTCCAA TAGGCACTCT 
TCAAAAACTC TTGGCCCAAA CCCTGGTCTT ATCCTTCAGG CCTTGACTCT GTCCAATGCA 
AGTGATGGTT TCAATCTGGA GCGTCTTGAA ATGCTTGGGG ACTCTTTCTT AAAACATGCT 
ATCACCACAT ATCTGTTCTG CACATACCCA GATGCTCACG AGGGCCGTCT ATCCTATATG 
CGCAGCAAGA AGGTCAGTAA CTGTAACCTT TATCGGCTTG GAAAAAAGAA AGGCTCACCC 
AGCCGGATGG TGGTGTCCAT TTTCGATCCA CCTGTCAACT GGCTTCCCCC TGGTTATATT 
GTTAACCAGG ACAAAAACTC TGATAAGTGG GAAAGTAATG AAACGTCTGG TGAGGATGTG 
ATGGTTAATG GCAAAATTGA TGAAGACTTT GATGATGAAG AAGATGAAGA TCTTATGTGG 
AGGAATCCCA AAGAGGAAAC TGATTTTGAT GATGACTTTT TGGAATATGA TCAAGAGCAT 
ATAAAATTCA TTGACAGCAT GTTAATGGGA TCTGGGGCAT TTGTGAAGAA AATTCCTCTT 
TCTTCATTTG CACCCCCTGA TCAGAACTAT GAATGGAGGG CGCCAAAGAA ACCTCCACTG 
GAAAGTTCTC AATTTCCCTG CGATTTTGAT GATTTTGATT ACAGTTCTTG GGATGCCATG 
TGTTATTTGG ATCCCAGCAA GGCTGTAGAG GAGGATGACT TTGTAGTAGG CTTTTGGAAT 
CCATCAGAAG AGAACTGTGG AGCTGATGCT GGAAAGCAGT CTATATCTTA TGACCTACAC 
ACAGAGCAGT GTATTGCAGA CAAAAGTATA GCTGACTGTG TAGAGGCTTT ATTAGGCTGC 
TATCTGACCA GTTGTGGTGA GAGAGCTGCA CAACTTTTTC TTTGTTCTTT GGGCTTAAAA 
GTGCTCCCAG AAGTAAGAAA GCTTGTTACA AACACAAATG TCATTAGTGC ATCATCATCT 
TACCAGAACA GTACCAGAGA CAACTGCACT CTTACTGCCA GAACCAACAC TGATCTGTCC 
TCCTGCAAAG GCATAGACTA CGGTTATTTA AAGATTCCCC CAAGGTGTAT GTTTGAGCAC 
CCAGATGCTG AGAAAACCCT TGACCATCTA ATATCTGGTT TTGAAAACTT TGAGAAGAAA 
ATAAACTATC CATTTAAAAA CAAGGCTTAC CTTCTACAGG CTTTTACACA TGCCTCTTAC 
CACTACAATA CTATAACTGA CTGCTACCAG CGTTTAGAAT TTCTTGGAGA TGCAATTTTG 
GACTACCTCA TTACTAAGCA CCTTTATGAA GATCCACGCC AGCACTCGCC AGGAGTCCTC 
ACTGACCTGC GCTCTGCTCT TGTGAATAAC ACCATATTTG CATCACTAGC TGTGAAATAT 
GACTACCATA AATACTTCAA GGCTATCTCT CCTGAACTGT TCCATGTAAT TGATGATTTT 
GTGCAATTTC AACTTGAAAA AAATGAAATG CAAGGCATGG ATTCTGAGTT ACGTAGGTCT 
GAAGAAGACG AAGAGAAAGA AGAGGACATT GAAGTCCCAA AAGCTATGGG AGACATATTT 
GAGTCACTTG CAGGAGCTAT TTACATGGAC AGCGGCATGT CTTTGGAAAC TGTATGGCAT 
GTGTATTATC CTATGATGCA GCCATTAATA GAAAAATTCT CTGCTAATGT TCCTCGTTCC 
CCAGTGAGAG AATTACTGGA AATGGAGCCT GAAACTGCTA AATTCAGTCC AGCCGAAAGA 
ACATACGATG GAAAAGTCAG AGTGACTGTG GAAGTTGTTG GAAAAGGAAA ATTTAAAGGA 
GTCGGAAGAA GTTACAGAAT TGCCAAATCT GCAGCAGCCA GGAGAGCACT AAGAAGCCTC 
AAAGCAAATC AATCTCAGGT CCCTAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dicer1 ( XM_031890234.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746094.1 Xenopus tropicalis dicer 1, ribonuclease III (dicer1), transcript variant X1, mRNA.

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CloneID	OXa63519
Clone ID Related Accession (Same CDS sequence)	XM_031890235.1 , XM_031890234.1
Accession Version	XM_031890234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCC TTCAGCTCAT GACTCCGGCC TCCTCTCCAA TGGGTCCCTT CTTTGGGCTA 
CCCTGGCAAC AGGAGGCTAT TCATGACAAC ATTTATACCC CACGGAAATA TCAGGTGGAA 
CTACTCGAAG CAGCATTGGA TCATAATACT ATAGTATGTT TGAATTCTGG CTCAGGGAAG 
ACATTTATTG CAGTACTACT CAGTAAAGAG CTGTCATACC AGATCCGTGG GGACTTCAGC 
AAAAATACTA AGAGGACTGT GTTTTTGGTC AATTCCGAGA AACAGGTTTC TCAACAAGTG 
TCTGCTGTCA GGACCCATAC AGACCTCAAG GTGGGAGAAT ATTCAGATCA AGAAAAAACA 
CAATGCTGGG CAAAAGAAAG ATGGTACCTA GAATTTGAAA CTCATCAGGT CTTGGTCATG 
ACCTGCCACA TCTTCCTGAA CGTTCTGAAA AGTGGGAACG TGTCATTGTC AAACATTAAT 
CTGTTAGTGT TTGATGAATG TCATCTTGCA ATTCAGGATC ACCCATATCG AGAAATCATG 
AAGATATGTG AGAGTTGCCA GCCATGCCCT CGAATCCTGG GTCTAACTGC TTCAATTTTA 
AATGGAAAAT GTGACCCTCG TGACCTAGAG GAAAAGATCC AGAAACTGGA GGAAATATTA 
AGGAGTAATG CAGAAACTGC AACTGATTTG GTTGTTTTAG ACAGGTATGC TTCCCAACCA 
TGTGAAATTG TATTGGACTG TGGGCCATAT ATTGACAAAA GTGGACTTTA TCAAAGACTT 
CTAAATGAAT TGGATGAAGC CCTTAACTTT CTCATTGACT GTAATATTTC TACACATTCT 
AAAGAGAGAG ATTCCACATT AATTTCAAAA CAGATTCTAT CAGACTGTCA AACTGTTCTT 
TTGGTCTTGG GACCATGGTG TGCTGATAAA GTTGCGGGGA TGATGGTGAG AGAGCTGCAG 
AAGTATATCA AACATGAGCA GGAGGAACTG CACAGAAAAT TCCTTTTGTT TACAGACACT 
ATCTTAAGGA AGATCCATGC TCTTTGTGAG GAACACTTCT CGCCAGCCTC TCTTGATATG 
AAGTTTGTCA CACCTAAAGT TATAAAACTG CTGGAAATTT TACGCAAATA CAAACCCTAC 
GAACGCCAGC AATTTGAAAG TGTTGAATGG TATAACAATA GAAACCAGGA TAATTATGTG 
TCTTGGAGTG ATTCAGAGGA TGATGACGAT GAAGATGAAG AAATTGAGGA GAAAGAGAAA 
ACTGAAACAA GCTTTCCATC CCCATTCACA AACATCCTGT GTGGCATCAT CTTCGTGGAA 
CGAAGATACA CAGCAGTAGT ATTAAACAGG TTGATTAAAG AAGCCGGGAA GCAAGATCCA 
GAGCTGGCCT ATATCAGTAG TAACTTTATT ACTGGGCACG GCATAGGAAA GAACCAGCCA 
CGCAATAAGC AGATGGAAGT TGAGTTTAGA AAGCAAGAAG AGGTGCTTCG TAAATTTCGT 
GCACACGAAA CCAACTTATT GATAGCTACT AGCATTGTTG AGGAAGGAGT GGACATACCA 
AAATGCAACT TGGTAGTTCG ATTTGATTTA CCTTCAGAGT ACAGATCCTA TGTACAGTCC 
AAAGGCAGAG CAAGAGCACC AATCTCAAAT TACATCATGC TAGCCGATAG TGATAAAATT 
AAGGCATTTG AAGAGGACCT TAAAACATAC AAAGCAATTG AAAAGATTCT GCGGAACAAA 
TGCTCAAAGT CCATTGATTG TGGAAATACA GAATCTGAGC CCATTGTGGA CGATGATGAA 
ATATTTCCAC CATATGTGTT GAGACAGGAT GATGGCAGCC CACGAGTTAC TATCAACACC 
GCTATTGGAC ACATTAACAG GTACTGTGCT AGGCTACCTA GTGACCCATT TACTCATCTT 
GCTCCTAAGT GCAAAACACG AGAGTTCCCT GATGGACTTT ATCGCTCAAC ACTCTACCTG 
CCAATTAATT CTCCACTTAG AGCCCCCATT GTTGGCCCTC CGATGAATTG TGGAAGGCTA 
GCTGATAGAG CTGTAGCTCT TATATGCTGT AAAAAACTAC ATGAAATTGG TGAACTGGAT 
GATCATTTAA TGCCAGTTGG CAAGGAAACT GTAAAATATG AGGAGGAGCT TGATTTGCAT 
GATGAGGAAG AAACCAGTGT TCCAGGCAGA CCAGGATCCA CAAAAAGAAG GCAGTGTTAT 
CCAAAAGCTA TTCCTGAATG TTTACGGAAC AGCTACCCCA AGCCTGGTCA GCCTTGTTAC 
TTATATGTAA TAGGAATGGT ATTAACCACT CCTCTACCAG ATGAACTTAA TTTTAGGCGA 
CGGAAGCTGT ATCCCCCTGA AGACACAACA AGATGCTTTG GAATACTAAC TGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTCTAT ACTCGTTCGG GAGAGGTGAC CATATCAATT 
GAACTAAAGA AGTCTGGTTT TACATTAAAC TTAGAACAAC TTGAGCTCAT TACTAGACTC 
CACCAGTACA TTTTTTCACA CATTCTTCGT CTTGAGAAAC CTGCACTAGA ATTTAAACCC 
ACAGTAGCCG ATTGTGCCTA CTGTGTTCTA CCTCTTAACG TTGTAAATGA TTCTGGCACT 
TTGGACATTG ACTTCAAATT TGTAGAAGAT ATTGAGAAAT CAGAAGCACG TACTGGTATA 
CCTAATACAC AGTATTCAGC TGAAAGTCCT TTTATTTTCA AATTAGAAGA CTACCAGGAT 
GCTGTTATCA TTCCAAGATA TCGAAATTTT GATCAGCCGC ACAGATTTTA TGTAGCTGAT 
GTATACACTG ATCTTACACC GCTCAGCAAG TTCCCTTCCC CTGAATATGA AACCTTTGCT 
GAATATTACA AAACAAAGTA TAACCTCGAT CTCACAAACC TCAATCAGCC ACTTTTGGAT 
GTGGACCACA CATCATCAAG ACTTAATTTG CTGACTCCTC GCCATCTGAA TCAGAAAGGT 
AAAGCTCTGC CATTAAGCAG TGCTGAAAAG AGAAAAGCCA AATGGGAGAG TTTACAAAAC 
AAACAGATCC TGGTTCCAGA GCTTTGTGCT ATACATCCAG TCCCAGCATC CTTATGGAGA 
AAAGCTGTTT GTTTGCCAAG CATACTTTAT CGATTACATT GCCTCCTTAC AGCAGAAGAG 
CTGAGAGCGC AAACAGCTAT TGATGCTGGG GTAGGAGTCA AATCGCTTCC TGATGATTTC 
CGATACCCAA ACTTGGATTT TGGATGGAAA CGATCTATAG ACAGTAAAAC ATTCATCTCT 
AATCAAAGTT CCTCATCAGT CGAGAGTGAA AGTGACTGCA GACTCAATAA AACCACGGCC 
CCTGACAGTG CTGCAAGCTC AGCTGCTAAT TCTGTAATCT ACATGCAAAT CAATGACCAA 
ATGTCTGTGA ACTGCACACC ACCATGTCAA AAATCTCTGA GTCACCTCCA AACAGTTTGC 
TTCTCAGATG ATTACAAAGC AATTAACGGT ATTTCTTGCA ATGGTCTTAC CAATGGTGAT 
TGGGAAGCAG AAAGCGCTGC ATGTTTCCAA AAGGACGAAC GGATAACCTG CAAACAGGAA 
ATACCTGAAA AATCTACCTC GTTTCACGTT CAGAATTTAC CTAAGGAGAA CCAGCCCATA 
CTTAAAGAAT GTACTCTGAG CAACTCTGAT GGAAATGTCA GCAAACCTAC CTCAGATGAG 
TGCCCTAGCA CCTGTACTTC AGACATGCAT TATGATAGTG GGCTTTCCAA TAGGCACTCT 
TCAAAAACTC TTGGCCCAAA CCCTGGTCTT ATCCTTCAGG CCTTGACTCT GTCCAATGCA 
AGTGATGGTT TCAATCTGGA GCGTCTTGAA ATGCTTGGGG ACTCTTTCTT AAAACATGCT 
ATCACCACAT ATCTGTTCTG CACATACCCA GATGCTCACG AGGGCCGTCT ATCCTATATG 
CGCAGCAAGA AGGTCAGTAA CTGTAACCTT TATCGGCTTG GAAAAAAGAA AGGCTCACCC 
AGCCGGATGG TGGTGTCCAT TTTCGATCCA CCTGTCAACT GGCTTCCCCC TGGTTATATT 
GTTAACCAGG ACAAAAACTC TGATAAGTGG GAAAGTAATG AAACGTCTGG TGAGGATGTG 
ATGGTTAATG GCAAAATTGA TGAAGACTTT GATGATGAAG AAGATGAAGA TCTTATGTGG 
AGGAATCCCA AAGAGGAAAC TGATTTTGAT GATGACTTTT TGGAATATGA TCAAGAGCAT 
ATAAAATTCA TTGACAGCAT GTTAATGGGA TCTGGGGCAT TTGTGAAGAA AATTCCTCTT 
TCTTCATTTG CACCCCCTGA TCAGAACTAT GAATGGAGGG CGCCAAAGAA ACCTCCACTG 
GAAAGTTCTC AATTTCCCTG CGATTTTGAT GATTTTGATT ACAGTTCTTG GGATGCCATG 
TGTTATTTGG ATCCCAGCAA GGCTGTAGAG GAGGATGACT TTGTAGTAGG CTTTTGGAAT 
CCATCAGAAG AGAACTGTGG AGCTGATGCT GGAAAGCAGT CTATATCTTA TGACCTACAC 
ACAGAGCAGT GTATTGCAGA CAAAAGTATA GCTGACTGTG TAGAGGCTTT ATTAGGCTGC 
TATCTGACCA GTTGTGGTGA GAGAGCTGCA CAACTTTTTC TTTGTTCTTT GGGCTTAAAA 
GTGCTCCCAG AAGTAAGAAA GCTTGTTACA AACACAAATG TCATTAGTGC ATCATCATCT 
TACCAGAACA GTACCAGAGA CAACTGCACT CTTACTGCCA GAACCAACAC TGATCTGTCC 
TCCTGCAAAG GCATAGACTA CGGTTATTTA AAGATTCCCC CAAGGTGTAT GTTTGAGCAC 
CCAGATGCTG AGAAAACCCT TGACCATCTA ATATCTGGTT TTGAAAACTT TGAGAAGAAA 
ATAAACTATC CATTTAAAAA CAAGGCTTAC CTTCTACAGG CTTTTACACA TGCCTCTTAC 
CACTACAATA CTATAACTGA CTGCTACCAG CGTTTAGAAT TTCTTGGAGA TGCAATTTTG 
GACTACCTCA TTACTAAGCA CCTTTATGAA GATCCACGCC AGCACTCGCC AGGAGTCCTC 
ACTGACCTGC GCTCTGCTCT TGTGAATAAC ACCATATTTG CATCACTAGC TGTGAAATAT 
GACTACCATA AATACTTCAA GGCTATCTCT CCTGAACTGT TCCATGTAAT TGATGATTTT 
GTGCAATTTC AACTTGAAAA AAATGAAATG CAAGGCATGG ATTCTGAGTT ACGTAGGTCT 
GAAGAAGACG AAGAGAAAGA AGAGGACATT GAAGTCCCAA AAGCTATGGG AGACATATTT 
GAGTCACTTG CAGGAGCTAT TTACATGGAC AGCGGCATGT CTTTGGAAAC TGTATGGCAT 
GTGTATTATC CTATGATGCA GCCATTAATA GAAAAATTCT CTGCTAATGT TCCTCGTTCC 
CCAGTGAGAG AATTACTGGA AATGGAGCCT GAAACTGCTA AATTCAGTCC AGCCGAAAGA 
ACATACGATG GAAAAGTCAG AGTGACTGTG GAAGTTGTTG GAAAAGGAAA ATTTAAAGGA 
GTCGGAAGAA GTTACAGAAT TGCCAAATCT GCAGCAGCCA GGAGAGCACT AAGAAGCCTC 
AAAGCAAATC AATCTCAGGT CCCTAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746094.1
CDS	362..6034
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNSGSGKTFIAVLLSKELSYQIRGDFSKNTKRTVFLVNSEKQVSQQVSAVRTHTDLKVGEYSDQEKTQCWAKERWYLEFETHQVLVMTCHIFLNVLKSGNVSLSNINLLVFDECHLAIQDHPYREIMKICESCQPCPRILGLTASILNGKCDPRDLEEKIQKLEEILRSNAETATDLVVLDRYASQPCEIVLDCGPYIDKSGLYQRLLNELDEALNFLIDCNISTHSKERDSTLISKQILSDCQTVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTILRKIHALCEEHFSPASLDMKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDEDEEIEEKEKTETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPSEYRSYVQSKGRARAPISNYIMLADSDKIKAFEEDLKTYKAIEKILRNKCSKSIDCGNTESEPIVDDDEIFPPYVLRQDDGSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTREFPDGLYRSTLYLPINSPLRAPIVGPPMNCGRLADRAVALICCKKLHEIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLNLEQLELITRLHQYIFSHILRLEKPALEFKPTVADCAYCVLPLNVVNDSGTLDIDFKFVEDIEKSEARTGIPNTQYSAESPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPVPASLWRKAVCLPSILYRLHCLLTAEELRAQTAIDAGVGVKSLPDDFRYPNLDFGWKRSIDSKTFISNQSSSSVESESDCRLNKTTAPDSAASSAANSVIYMQINDQMSVNCTPPCQKSLSHLQTVCFSDDYKAINGISCNGLTNGDWEAESAACFQKDERITCKQEIPEKSTSFHVQNLPKENQPILKECTLSNSDGNVSKPTSDECPSTCTSDMHYDSGLSNRHSSKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGSPSRMVVSIFDPPVNWLPPGYIVNQDKNSDKWESNETSGEDVMVNGKIDEDFDDEEDEDLMWRNPKEETDFDDDFLEYDQEHIKFIDSMLMGSGAFVKKIPLSSFAPPDQNYEWRAPKKPPLESSQFPCDFDDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGADAGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPEVRKLVTNTNVISASSSYQNSTRDNCTLTARTNTDLSSCKGIDYGYLKIPPRCMFEHPDAEKTLDHLISGFENFEKKINYPFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWHVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQSQVPNS

Target ORF information:

RefSeq Version	XM_031890234.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis dicer 1, ribonuclease III (dicer1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890234.1

ORF Insert Sequence:

ATGGCAGGCC TTCAGCTCAT GACTCCGGCC TCCTCTCCAA TGGGTCCCTT CTTTGGGCTA 
CCCTGGCAAC AGGAGGCTAT TCATGACAAC ATTTATACCC CACGGAAATA TCAGGTGGAA 
CTACTCGAAG CAGCATTGGA TCATAATACT ATAGTATGTT TGAATTCTGG CTCAGGGAAG 
ACATTTATTG CAGTACTACT CAGTAAAGAG CTGTCATACC AGATCCGTGG GGACTTCAGC 
AAAAATACTA AGAGGACTGT GTTTTTGGTC AATTCCGAGA AACAGGTTTC TCAACAAGTG 
TCTGCTGTCA GGACCCATAC AGACCTCAAG GTGGGAGAAT ATTCAGATCA AGAAAAAACA 
CAATGCTGGG CAAAAGAAAG ATGGTACCTA GAATTTGAAA CTCATCAGGT CTTGGTCATG 
ACCTGCCACA TCTTCCTGAA CGTTCTGAAA AGTGGGAACG TGTCATTGTC AAACATTAAT 
CTGTTAGTGT TTGATGAATG TCATCTTGCA ATTCAGGATC ACCCATATCG AGAAATCATG 
AAGATATGTG AGAGTTGCCA GCCATGCCCT CGAATCCTGG GTCTAACTGC TTCAATTTTA 
AATGGAAAAT GTGACCCTCG TGACCTAGAG GAAAAGATCC AGAAACTGGA GGAAATATTA 
AGGAGTAATG CAGAAACTGC AACTGATTTG GTTGTTTTAG ACAGGTATGC TTCCCAACCA 
TGTGAAATTG TATTGGACTG TGGGCCATAT ATTGACAAAA GTGGACTTTA TCAAAGACTT 
CTAAATGAAT TGGATGAAGC CCTTAACTTT CTCATTGACT GTAATATTTC TACACATTCT 
AAAGAGAGAG ATTCCACATT AATTTCAAAA CAGATTCTAT CAGACTGTCA AACTGTTCTT 
TTGGTCTTGG GACCATGGTG TGCTGATAAA GTTGCGGGGA TGATGGTGAG AGAGCTGCAG 
AAGTATATCA AACATGAGCA GGAGGAACTG CACAGAAAAT TCCTTTTGTT TACAGACACT 
ATCTTAAGGA AGATCCATGC TCTTTGTGAG GAACACTTCT CGCCAGCCTC TCTTGATATG 
AAGTTTGTCA CACCTAAAGT TATAAAACTG CTGGAAATTT TACGCAAATA CAAACCCTAC 
GAACGCCAGC AATTTGAAAG TGTTGAATGG TATAACAATA GAAACCAGGA TAATTATGTG 
TCTTGGAGTG ATTCAGAGGA TGATGACGAT GAAGATGAAG AAATTGAGGA GAAAGAGAAA 
ACTGAAACAA GCTTTCCATC CCCATTCACA AACATCCTGT GTGGCATCAT CTTCGTGGAA 
CGAAGATACA CAGCAGTAGT ATTAAACAGG TTGATTAAAG AAGCCGGGAA GCAAGATCCA 
GAGCTGGCCT ATATCAGTAG TAACTTTATT ACTGGGCACG GCATAGGAAA GAACCAGCCA 
CGCAATAAGC AGATGGAAGT TGAGTTTAGA AAGCAAGAAG AGGTGCTTCG TAAATTTCGT 
GCACACGAAA CCAACTTATT GATAGCTACT AGCATTGTTG AGGAAGGAGT GGACATACCA 
AAATGCAACT TGGTAGTTCG ATTTGATTTA CCTTCAGAGT ACAGATCCTA TGTACAGTCC 
AAAGGCAGAG CAAGAGCACC AATCTCAAAT TACATCATGC TAGCCGATAG TGATAAAATT 
AAGGCATTTG AAGAGGACCT TAAAACATAC AAAGCAATTG AAAAGATTCT GCGGAACAAA 
TGCTCAAAGT CCATTGATTG TGGAAATACA GAATCTGAGC CCATTGTGGA CGATGATGAA 
ATATTTCCAC CATATGTGTT GAGACAGGAT GATGGCAGCC CACGAGTTAC TATCAACACC 
GCTATTGGAC ACATTAACAG GTACTGTGCT AGGCTACCTA GTGACCCATT TACTCATCTT 
GCTCCTAAGT GCAAAACACG AGAGTTCCCT GATGGACTTT ATCGCTCAAC ACTCTACCTG 
CCAATTAATT CTCCACTTAG AGCCCCCATT GTTGGCCCTC CGATGAATTG TGGAAGGCTA 
GCTGATAGAG CTGTAGCTCT TATATGCTGT AAAAAACTAC ATGAAATTGG TGAACTGGAT 
GATCATTTAA TGCCAGTTGG CAAGGAAACT GTAAAATATG AGGAGGAGCT TGATTTGCAT 
GATGAGGAAG AAACCAGTGT TCCAGGCAGA CCAGGATCCA CAAAAAGAAG GCAGTGTTAT 
CCAAAAGCTA TTCCTGAATG TTTACGGAAC AGCTACCCCA AGCCTGGTCA GCCTTGTTAC 
TTATATGTAA TAGGAATGGT ATTAACCACT CCTCTACCAG ATGAACTTAA TTTTAGGCGA 
CGGAAGCTGT ATCCCCCTGA AGACACAACA AGATGCTTTG GAATACTAAC TGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTCTAT ACTCGTTCGG GAGAGGTGAC CATATCAATT 
GAACTAAAGA AGTCTGGTTT TACATTAAAC TTAGAACAAC TTGAGCTCAT TACTAGACTC 
CACCAGTACA TTTTTTCACA CATTCTTCGT CTTGAGAAAC CTGCACTAGA ATTTAAACCC 
ACAGTAGCCG ATTGTGCCTA CTGTGTTCTA CCTCTTAACG TTGTAAATGA TTCTGGCACT 
TTGGACATTG ACTTCAAATT TGTAGAAGAT ATTGAGAAAT CAGAAGCACG TACTGGTATA 
CCTAATACAC AGTATTCAGC TGAAAGTCCT TTTATTTTCA AATTAGAAGA CTACCAGGAT 
GCTGTTATCA TTCCAAGATA TCGAAATTTT GATCAGCCGC ACAGATTTTA TGTAGCTGAT 
GTATACACTG ATCTTACACC GCTCAGCAAG TTCCCTTCCC CTGAATATGA AACCTTTGCT 
GAATATTACA AAACAAAGTA TAACCTCGAT CTCACAAACC TCAATCAGCC ACTTTTGGAT 
GTGGACCACA CATCATCAAG ACTTAATTTG CTGACTCCTC GCCATCTGAA TCAGAAAGGT 
AAAGCTCTGC CATTAAGCAG TGCTGAAAAG AGAAAAGCCA AATGGGAGAG TTTACAAAAC 
AAACAGATCC TGGTTCCAGA GCTTTGTGCT ATACATCCAG TCCCAGCATC CTTATGGAGA 
AAAGCTGTTT GTTTGCCAAG CATACTTTAT CGATTACATT GCCTCCTTAC AGCAGAAGAG 
CTGAGAGCGC AAACAGCTAT TGATGCTGGG GTAGGAGTCA AATCGCTTCC TGATGATTTC 
CGATACCCAA ACTTGGATTT TGGATGGAAA CGATCTATAG ACAGTAAAAC ATTCATCTCT 
AATCAAAGTT CCTCATCAGT CGAGAGTGAA AGTGACTGCA GACTCAATAA AACCACGGCC 
CCTGACAGTG CTGCAAGCTC AGCTGCTAAT TCTGTAATCT ACATGCAAAT CAATGACCAA 
ATGTCTGTGA ACTGCACACC ACCATGTCAA AAATCTCTGA GTCACCTCCA AACAGTTTGC 
TTCTCAGATG ATTACAAAGC AATTAACGGT ATTTCTTGCA ATGGTCTTAC CAATGGTGAT 
TGGGAAGCAG AAAGCGCTGC ATGTTTCCAA AAGGACGAAC GGATAACCTG CAAACAGGAA 
ATACCTGAAA AATCTACCTC GTTTCACGTT CAGAATTTAC CTAAGGAGAA CCAGCCCATA 
CTTAAAGAAT GTACTCTGAG CAACTCTGAT GGAAATGTCA GCAAACCTAC CTCAGATGAG 
TGCCCTAGCA CCTGTACTTC AGACATGCAT TATGATAGTG GGCTTTCCAA TAGGCACTCT 
TCAAAAACTC TTGGCCCAAA CCCTGGTCTT ATCCTTCAGG CCTTGACTCT GTCCAATGCA 
AGTGATGGTT TCAATCTGGA GCGTCTTGAA ATGCTTGGGG ACTCTTTCTT AAAACATGCT 
ATCACCACAT ATCTGTTCTG CACATACCCA GATGCTCACG AGGGCCGTCT ATCCTATATG 
CGCAGCAAGA AGGTCAGTAA CTGTAACCTT TATCGGCTTG GAAAAAAGAA AGGCTCACCC 
AGCCGGATGG TGGTGTCCAT TTTCGATCCA CCTGTCAACT GGCTTCCCCC TGGTTATATT 
GTTAACCAGG ACAAAAACTC TGATAAGTGG GAAAGTAATG AAACGTCTGG TGAGGATGTG 
ATGGTTAATG GCAAAATTGA TGAAGACTTT GATGATGAAG AAGATGAAGA TCTTATGTGG 
AGGAATCCCA AAGAGGAAAC TGATTTTGAT GATGACTTTT TGGAATATGA TCAAGAGCAT 
ATAAAATTCA TTGACAGCAT GTTAATGGGA TCTGGGGCAT TTGTGAAGAA AATTCCTCTT 
TCTTCATTTG CACCCCCTGA TCAGAACTAT GAATGGAGGG CGCCAAAGAA ACCTCCACTG 
GAAAGTTCTC AATTTCCCTG CGATTTTGAT GATTTTGATT ACAGTTCTTG GGATGCCATG 
TGTTATTTGG ATCCCAGCAA GGCTGTAGAG GAGGATGACT TTGTAGTAGG CTTTTGGAAT 
CCATCAGAAG AGAACTGTGG AGCTGATGCT GGAAAGCAGT CTATATCTTA TGACCTACAC 
ACAGAGCAGT GTATTGCAGA CAAAAGTATA GCTGACTGTG TAGAGGCTTT ATTAGGCTGC 
TATCTGACCA GTTGTGGTGA GAGAGCTGCA CAACTTTTTC TTTGTTCTTT GGGCTTAAAA 
GTGCTCCCAG AAGTAAGAAA GCTTGTTACA AACACAAATG TCATTAGTGC ATCATCATCT 
TACCAGAACA GTACCAGAGA CAACTGCACT CTTACTGCCA GAACCAACAC TGATCTGTCC 
TCCTGCAAAG GCATAGACTA CGGTTATTTA AAGATTCCCC CAAGGTGTAT GTTTGAGCAC 
CCAGATGCTG AGAAAACCCT TGACCATCTA ATATCTGGTT TTGAAAACTT TGAGAAGAAA 
ATAAACTATC CATTTAAAAA CAAGGCTTAC CTTCTACAGG CTTTTACACA TGCCTCTTAC 
CACTACAATA CTATAACTGA CTGCTACCAG CGTTTAGAAT TTCTTGGAGA TGCAATTTTG 
GACTACCTCA TTACTAAGCA CCTTTATGAA GATCCACGCC AGCACTCGCC AGGAGTCCTC 
ACTGACCTGC GCTCTGCTCT TGTGAATAAC ACCATATTTG CATCACTAGC TGTGAAATAT 
GACTACCATA AATACTTCAA GGCTATCTCT CCTGAACTGT TCCATGTAAT TGATGATTTT 
GTGCAATTTC AACTTGAAAA AAATGAAATG CAAGGCATGG ATTCTGAGTT ACGTAGGTCT 
GAAGAAGACG AAGAGAAAGA AGAGGACATT GAAGTCCCAA AAGCTATGGG AGACATATTT 
GAGTCACTTG CAGGAGCTAT TTACATGGAC AGCGGCATGT CTTTGGAAAC TGTATGGCAT 
GTGTATTATC CTATGATGCA GCCATTAATA GAAAAATTCT CTGCTAATGT TCCTCGTTCC 
CCAGTGAGAG AATTACTGGA AATGGAGCCT GAAACTGCTA AATTCAGTCC AGCCGAAAGA 
ACATACGATG GAAAAGTCAG AGTGACTGTG GAAGTTGTTG GAAAAGGAAA ATTTAAAGGA 
GTCGGAAGAA GTTACAGAAT TGCCAAATCT GCAGCAGCCA GGAGAGCACT AAGAAGCCTC 
AAAGCAAATC AATCTCAGGT CCCTAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dicer1 ( NM_001129918.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001123390.2 Xenopus tropicalis dicer 1, ribonuclease III (dicer1), mRNA.

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CloneID	OXa25838
Clone ID Related Accession (Same CDS sequence)	NM_001129918.2
Accession Version	NM_001129918.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Xenopus tropicalis(tropical clawed frog)
Product	endoribonuclease Dicer
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU338242.1. On Jul 18, 2009 this sequence version replaced NM_001129918.1. ##Evidence-Data-START## Transcript exon combination :: EU338242.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGGCC TTCAGCTCAT GACTCCGGCC TCCTCTCCAA TGGGTCCCTT CTTTGGGCTA 
CCCTGGCAAC AGGAGGCTAT TCATGACAAC ATTTATACCC CACGGAAATA TCAGGTGGAA 
CTACTCGAAG CAGCATTGGA TCATAATACT ATAGTATGTT TGAATTCTGG CTCAGGGAAG 
ACATTTATTG CAGTACTACT CAGTAAAGAG CTGTCATACC AGATCCGTGG GGACTTCAGC 
AAAAATACTA AGAGGACTGT GTTTTTGGTC AATTCCGAGA AACAGGTTTC TCAACAAGTG 
TCTGCTGTCA GGACCCATAC AGACCTCAAG GTGGGAGAAT ATTCAGATCA AGAAAAAACA 
CAATGCTGGG CAAAAGAAAG ATGGTACCTA GAATTTGAAA CTCATCAGGT CTTGGTCATG 
ACCTGCCACA TCTTCCTGAA CGTTCTGAAA AGTGGGAACG TGTCATTGTC AAACATTAAT 
CTGTTAGTGT TTGATGAATG TCATCTTGCA ATTCAGGATC ACCCATATCG AGAAATCATG 
AAGATATGTG AGAGTTGCCA GCCATGCCCT CGAATCCTGG GTCTAACTGC TTCAATTTTA 
AATGGAAAAT GTGACCCTCG TGACCTAGAG GAAAAGATCC AGAAACTGGA GGAAATATTA 
AGGAGTAATG CAGAAACTGC AACTGATTTG GTTGTTTTAG ACAGGTATGC TTCCCAACCA 
TGTGAAATTG TATTGGACTG TGGGCCATAT ATTGACAAAA GTGGACTTTA TCAAAGACTT 
CTAAATGAAT TGGATGAAGC CCTTAACTTT CTCATTGACT GTAATATTTC TACACATTCT 
AAAGAGAGAG ATTCCACATT AATTTCAAAA CAGATTCTAT CAGACTGTCA AACTGTTCTT 
TTGGTCTTGG GACCATGGTG TGCTGATAAA GTTGCGGGGA TGATGGTGAG AGAGCTGCAG 
AAGTATATCA AACATGAGCA GGAGGAACTG CACAGAAAAT TCCTTTTGTT TACAGACACT 
ATCTTAAGGA AGATCCATGC TCTTTGTGAG GAACACTTCT CGCCAGCCTC TCTTGATATG 
AAGTTTGTCA CACCTAAAGT TATAAAACTG CTGGAAATTT TACGCAAATA CAAACCCTAC 
GAACGCCAGC AATTTGAAAG TGTTGAATGG TATAACAATA GAAACCAGGA TAATTATGTG 
TCTTGGAGTG ATTCAGAGGA TGATGACGAT GAAGATGAAG AAATTGAGGA GAAAGAGAAA 
ACTGAAACAA GCTTTCCATC CCCATTCACA AACATCCTGT GTGGCATCAT CTTCGTGGAA 
CGAAGATACA CAGCAGTAGT ATTAAACAGG TTGATTAAAG AAGCCGGGAA GCAAGATCCA 
GAGCTGGCCT ATATCAGTAG TAACTTTATT ACTGGGCACG GCATAGGAAA GAACCAGCCA 
CGCAATAAGC AGATGGAAGT TGAGTTTAGA AAGCAAGAAG AGGTGCTTCG TAAATTTCGT 
GCACACGAAA CCAACTTATT GATAGCTACT AGCATTGTTG AGGAAGGAGT GGACATACCA 
AAATGCAACT TGGTAGTTCG ATTTGATTTA CCTTCAGAGT ACAGATCCTA TGTACAGTCC 
AAAGGCAGAG CAAGAGCACC AATCTCAAAT TACATCATGC TAGCCGATAG TGATAAAATT 
AAGGCATTTG AAGAGGACCT TAAAACATAC AAAGCAATTG AAAAGATTCT GCGGAACAAA 
TGCTCAAAGT CCATTGATTG TGGAAATACA GAATCTGAGC CCATTGTGGA CGATGATGAA 
ATATTTCCAC CATATGTGTT GAGACAGGAT GATGGCAGCC CACGAGTTAC TATCAACACC 
GCTATTGGAC ACATTAACAG GTACTGTGCT AGGCTACCTA GTGACCCATT TACTCATCTT 
GCTCCTAAGT GCAAAACACG AGAGTTCCCT GATGGACTTT ATCGCTCAAC ACTCTACCTG 
CCAATTAATT CTCCACTTAG AGCCCCCATT GTTGGCCCTC CGATGAATTG TGGAAGGCTA 
GCTGATAGAG CTGTAGCTCT TATATGCTGT AAAAAACTAC ATGAAATTGG TGAACTGGAT 
GATCATTTAA TGCCAGTTGG CAAGGAAACT GTAAAATATG AGGAGGAGCT TGATTTGCAT 
GATGAGGAAG AAACCAGTGT TCCAGGCAGA CCAGGATCCA CAAAAAGAAG GCAGTGTTAT 
CCAAAAGCTA TTCCTGAATG TTTACGGAAC AGCTACCCCA AGCCTGGTCA GCCTTGTTAC 
TTATATGTAA TAGGAATGGT ATTAACCACT CCTCTACCAG ATGAACTTAA TTTTAGGCGA 
CGGAAGCTGT ATCCCCCTGA AGACACAACA AGATGCTTTG GAATACTAAC TGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTCTAT ACTCGTTCGG GAGAGGTGAC CATATCAATT 
GAACTAAAGA AGTCTGGTTT TACATTAAAC TTAGAACAAC TTGAGCTCAT TACTAGACTC 
CACCAGTACA TTTTTTCACA CATTCTTCGT CTTGAGAAAC CTGCACTAGA ATTTAAACCC 
ACAGTAGCCG ATTGTGCCTA CTGTGTTCTA CCTCTTAACG TTGTAAATGA TTCTGGCACT 
TTGGACATTG ACTTCAAATT TGTAGAAGAT ATTGAGAAAT CAGAAGCACG TACTGGTATA 
CCTAATACAC AGTATTCAGC TGAAAGTCCT TTTATTTTCA AATTAGAAGA CTACCAGGAT 
GCTGTTATCA TTCCAAGGCA AGTAATATAT CGAAATTTTG ATCAGCCGCA CAGATTTTAT 
GTAGCTGATG TATACACTGA TCTTACACCG CTCAGCAAGT TCCCTTCCCC TGAATATGAA 
ACCTTTGCTG AATATTACAA AACAAAGTAT AACCTCGATC TCACAAACCT CAATCAGCCA 
CTTTTGGATG TGGACCACAC ATCATCAAGA CTTAATTTGC TGACTCCTCG CCATCTGAAT 
CAGAAAGGTA AAGCTCTGCC ATTAAGCAGT GCTGAAAAGA GAAAAGCCAA ATGGGAGAGT 
TTACAAAACA AACAGATCCT GGTTCCAGAG CTTTGTGCTA TACATCCAGT CCCAGCATCC 
TTATGGAGAA AAGCTGTTTG TTTGCCAAGC ATACTTTATC GATTACATTG CCTCCTTACA 
GCAGAAGAGC TGAGAGCGCA AACAGCTATT GATGCTGGGG TAGGAGTCAA ATCGCTTCCT 
GATGATTTCC GATACCCAAA CTTGGATTTT GGATGGAAAC GATCTATAGA CAGTAAAACA 
TTCATCTCTA ATCAAAGTTC CTCATCAGTC GAGAGTGAAA GTGACTGCAG ACTCAATAAA 
ACCACGGCCC CTGACAGTGC TGCAAGCTCA GCTGCTAATT CTGTAATCTA CATGCAAATC 
AATGACCAAA TGTCTGTGAA CTGCACACCA CCATGTCAAA AATCTCTGAG TCACCTCCAA 
ACAGTTTGCT TCTCAGATGA TTACAAAGCA ATTAACGGTA TTTCTTGCAA TGGTCTTACC 
AATGGTGATT GGGAAGCAGA AAGCGCTGCA TGTTTCCAAA AGGACGAACG GATAACCTGC 
AAACAGGAAA TACCTGAAAA ATCTACCTCG TTTCACGTTC AGAATTTACC TAAGGAGAAC 
CAGCCCATAC TTAAAGAATG TACTCTGAGC AACTCTGATG GAAATGTCAG CAAACCTACC 
TCAGATGAGT GCCCTAGCAC CTGTACTTCA GACATGCATT ATGATAGTGG GCTTTCCAAT 
AGGCACTCTT CAAAAACTCT TGGCCCAAAC CCTGGTCTTA TCCTTCAGGC CTTGACTCTG 
TCCAATGCAA GTGATGGTTT CAATCTGGAG CGTCTTGAAA TGCTTGGGGA CTCTTTCTTA 
AAACATGCTA TCACCACATA TCTGTTCTGC ACATACCCAG ATGCTCACGA GGGCCGTCTA 
TCCTATATGC GCAGCAAGAA GGTCAGTAAC TGTAACCTTT ATCGGCTTGG AAAAAAGAAA 
GGCTCACCCA GCCGGATGGT GGTGTCCATT TTCGATCCAC CTGTCAACTG GCTTCCCCCT 
GGTTATATTG TTAACCAGGA CAAAAACTCT GATAAGTGGG AAAGTAATGA AACGTCTGGT 
GAGGATGTGA TGGTTAATGG CAAAATTGAT GAAGACTTTG ATGATGAAGA AGATGAAGAT 
CTTATGTGGA GGAATCCCAA AGAGGAAACT GATTTTGATG ATGACTTTTT GGAATATGAT 
CAAGAGCATA TAAAATTCAT TGACAGCATG TTAATGGGAT CTGGGGCATT TGTGAAGAAA 
ATTCCTCTTT CTTCATTTGC ACCCCCTGAT CAGAACTATG AATGGAGGGC GCCAAAGAAA 
CCTCCACTGG AAAGTTCTCA ATTTCCCTGC GATTTTGATG ATTTTGATTA CAGTTCTTGG 
GATGCCATGT GTTATTTGGA TCCCAGCAAG GCTGTAGAGG AGGATGACTT TGTAGTAGGC 
TTTTGGAATC CATCAGAAGA GAACTGTGGA GCTGATGCTG GAAAGCAGTC TATATCTTAT 
GACCTACACA CAGAGCAGTG TATTGCAGAC AAAAGTATAG CTGACTGTGT AGAGGCTTTA 
TTAGGCTGCT ATCTGACCAG TTGTGGTGAG AGAGCTGCAC AACTGTTTCT TTGTTCTTTG 
GGCTTAAAAG TGCTCCCAGA AGTAAGAAAG CTTGTTACAA ACACAAATGT CATTAGTGCA 
TCATCATCTT ACCAGAACAG TACCAGAGAC AACTGCACTC TTACTGCCAG AACCAACACT 
GATCTGTCCT CCTGCAAAGG CATAGACTAC GGTTATTTAA AGATTCCCCC AAGGTGTATG 
TTTGAGCACC CAGATGCTGA GAAAACCCTT GACCATCTAA TATCTGGTTT TGAAAACTTT 
GAGAAGAAAA TAAACTATCC ATTTAAAAAC AAGGCTTACC TTCTACAGGC TTTTACACAT 
GCCTCTTACC ACTACAATAC TATAACTGAC TGCTACCAGC GTTTAGAATT TCTTGGAGAT 
GCAATTTTGG ACTACCTCAT TACTAAGCAC CTTTATGAAG ATCCACGCCA GCACTCGCCA 
GGAGTCCTCA CTGACCTGCG CTCTGCTCTT GTGAATAACA CCATATTTGC ATCACTAGCT 
GTGAAATATG ACTACCATAA ATACTTCAAG GCTATCTCTC CTGAACTGTT CCATGTAATT 
GATGATTTTG TGCAATTTCA ACTTGAAAAA AATGAAATGC AAGGCATGGA TTCTGAGTTA 
CGTAGGTCTG AAGAAGACGA AGAGAAAGAA GAGGACATTG AAGTCCCAAA AGCTATGGGA 
GACATATTTG AGTCACTTGC AGGAGCTATT TACATGGACA GCGGCATGTC TTTGGAAACT 
GTATGGCATG TGTATTATCC TATGATGCAG CCATTAATAG AAAAATTCTC TGCTAATGTT 
CCTCGTTCCC CAGTGAGAGA ATTACTGGAA ATGGAGCCTG AAACTGCTAA ATTCAGTCCA 
GCCGAAAGAA CATACGATGG AAAAGTCAGA GTGACTGTGG AAGTTGTTGG AAAAGGAAAA 
TTTAAAGGAG TCGGAAGAAG TTACAGAATT GCCAAATCTG CAGCAGCCAG GAGAGCACTA 
AGAAGCCTCA AAGCAAATCA ATCTCAGGTC CCTAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123390.2
CDS	1..5682
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNSGSGKTFIAVLLSKELSYQIRGDFSKNTKRTVFLVNSEKQVSQQVSAVRTHTDLKVGEYSDQEKTQCWAKERWYLEFETHQVLVMTCHIFLNVLKSGNVSLSNINLLVFDECHLAIQDHPYREIMKICESCQPCPRILGLTASILNGKCDPRDLEEKIQKLEEILRSNAETATDLVVLDRYASQPCEIVLDCGPYIDKSGLYQRLLNELDEALNFLIDCNISTHSKERDSTLISKQILSDCQTVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTILRKIHALCEEHFSPASLDMKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDEDEEIEEKEKTETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPSEYRSYVQSKGRARAPISNYIMLADSDKIKAFEEDLKTYKAIEKILRNKCSKSIDCGNTESEPIVDDDEIFPPYVLRQDDGSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTREFPDGLYRSTLYLPINSPLRAPIVGPPMNCGRLADRAVALICCKKLHEIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLNLEQLELITRLHQYIFSHILRLEKPALEFKPTVADCAYCVLPLNVVNDSGTLDIDFKFVEDIEKSEARTGIPNTQYSAESPFIFKLEDYQDAVIIPRQVIYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPVPASLWRKAVCLPSILYRLHCLLTAEELRAQTAIDAGVGVKSLPDDFRYPNLDFGWKRSIDSKTFISNQSSSSVESESDCRLNKTTAPDSAASSAANSVIYMQINDQMSVNCTPPCQKSLSHLQTVCFSDDYKAINGISCNGLTNGDWEAESAACFQKDERITCKQEIPEKSTSFHVQNLPKENQPILKECTLSNSDGNVSKPTSDECPSTCTSDMHYDSGLSNRHSSKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGSPSRMVVSIFDPPVNWLPPGYIVNQDKNSDKWESNETSGEDVMVNGKIDEDFDDEEDEDLMWRNPKEETDFDDDFLEYDQEHIKFIDSMLMGSGAFVKKIPLSSFAPPDQNYEWRAPKKPPLESSQFPCDFDDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGADAGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPEVRKLVTNTNVISASSSYQNSTRDNCTLTARTNTDLSSCKGIDYGYLKIPPRCMFEHPDAEKTLDHLISGFENFEKKINYPFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWHVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQSQVPNS

Target ORF information:

RefSeq Version	NM_001129918.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis dicer 1, ribonuclease III (dicer1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129918.2

ORF Insert Sequence:

ATGGCAGGCC TTCAGCTCAT GACTCCGGCC TCCTCTCCAA TGGGTCCCTT CTTTGGGCTA 
CCCTGGCAAC AGGAGGCTAT TCATGACAAC ATTTATACCC CACGGAAATA TCAGGTGGAA 
CTACTCGAAG CAGCATTGGA TCATAATACT ATAGTATGTT TGAATTCTGG CTCAGGGAAG 
ACATTTATTG CAGTACTACT CAGTAAAGAG CTGTCATACC AGATCCGTGG GGACTTCAGC 
AAAAATACTA AGAGGACTGT GTTTTTGGTC AATTCCGAGA AACAGGTTTC TCAACAAGTG 
TCTGCTGTCA GGACCCATAC AGACCTCAAG GTGGGAGAAT ATTCAGATCA AGAAAAAACA 
CAATGCTGGG CAAAAGAAAG ATGGTACCTA GAATTTGAAA CTCATCAGGT CTTGGTCATG 
ACCTGCCACA TCTTCCTGAA CGTTCTGAAA AGTGGGAACG TGTCATTGTC AAACATTAAT 
CTGTTAGTGT TTGATGAATG TCATCTTGCA ATTCAGGATC ACCCATATCG AGAAATCATG 
AAGATATGTG AGAGTTGCCA GCCATGCCCT CGAATCCTGG GTCTAACTGC TTCAATTTTA 
AATGGAAAAT GTGACCCTCG TGACCTAGAG GAAAAGATCC AGAAACTGGA GGAAATATTA 
AGGAGTAATG CAGAAACTGC AACTGATTTG GTTGTTTTAG ACAGGTATGC TTCCCAACCA 
TGTGAAATTG TATTGGACTG TGGGCCATAT ATTGACAAAA GTGGACTTTA TCAAAGACTT 
CTAAATGAAT TGGATGAAGC CCTTAACTTT CTCATTGACT GTAATATTTC TACACATTCT 
AAAGAGAGAG ATTCCACATT AATTTCAAAA CAGATTCTAT CAGACTGTCA AACTGTTCTT 
TTGGTCTTGG GACCATGGTG TGCTGATAAA GTTGCGGGGA TGATGGTGAG AGAGCTGCAG 
AAGTATATCA AACATGAGCA GGAGGAACTG CACAGAAAAT TCCTTTTGTT TACAGACACT 
ATCTTAAGGA AGATCCATGC TCTTTGTGAG GAACACTTCT CGCCAGCCTC TCTTGATATG 
AAGTTTGTCA CACCTAAAGT TATAAAACTG CTGGAAATTT TACGCAAATA CAAACCCTAC 
GAACGCCAGC AATTTGAAAG TGTTGAATGG TATAACAATA GAAACCAGGA TAATTATGTG 
TCTTGGAGTG ATTCAGAGGA TGATGACGAT GAAGATGAAG AAATTGAGGA GAAAGAGAAA 
ACTGAAACAA GCTTTCCATC CCCATTCACA AACATCCTGT GTGGCATCAT CTTCGTGGAA 
CGAAGATACA CAGCAGTAGT ATTAAACAGG TTGATTAAAG AAGCCGGGAA GCAAGATCCA 
GAGCTGGCCT ATATCAGTAG TAACTTTATT ACTGGGCACG GCATAGGAAA GAACCAGCCA 
CGCAATAAGC AGATGGAAGT TGAGTTTAGA AAGCAAGAAG AGGTGCTTCG TAAATTTCGT 
GCACACGAAA CCAACTTATT GATAGCTACT AGCATTGTTG AGGAAGGAGT GGACATACCA 
AAATGCAACT TGGTAGTTCG ATTTGATTTA CCTTCAGAGT ACAGATCCTA TGTACAGTCC 
AAAGGCAGAG CAAGAGCACC AATCTCAAAT TACATCATGC TAGCCGATAG TGATAAAATT 
AAGGCATTTG AAGAGGACCT TAAAACATAC AAAGCAATTG AAAAGATTCT GCGGAACAAA 
TGCTCAAAGT CCATTGATTG TGGAAATACA GAATCTGAGC CCATTGTGGA CGATGATGAA 
ATATTTCCAC CATATGTGTT GAGACAGGAT GATGGCAGCC CACGAGTTAC TATCAACACC 
GCTATTGGAC ACATTAACAG GTACTGTGCT AGGCTACCTA GTGACCCATT TACTCATCTT 
GCTCCTAAGT GCAAAACACG AGAGTTCCCT GATGGACTTT ATCGCTCAAC ACTCTACCTG 
CCAATTAATT CTCCACTTAG AGCCCCCATT GTTGGCCCTC CGATGAATTG TGGAAGGCTA 
GCTGATAGAG CTGTAGCTCT TATATGCTGT AAAAAACTAC ATGAAATTGG TGAACTGGAT 
GATCATTTAA TGCCAGTTGG CAAGGAAACT GTAAAATATG AGGAGGAGCT TGATTTGCAT 
GATGAGGAAG AAACCAGTGT TCCAGGCAGA CCAGGATCCA CAAAAAGAAG GCAGTGTTAT 
CCAAAAGCTA TTCCTGAATG TTTACGGAAC AGCTACCCCA AGCCTGGTCA GCCTTGTTAC 
TTATATGTAA TAGGAATGGT ATTAACCACT CCTCTACCAG ATGAACTTAA TTTTAGGCGA 
CGGAAGCTGT ATCCCCCTGA AGACACAACA AGATGCTTTG GAATACTAAC TGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTCTAT ACTCGTTCGG GAGAGGTGAC CATATCAATT 
GAACTAAAGA AGTCTGGTTT TACATTAAAC TTAGAACAAC TTGAGCTCAT TACTAGACTC 
CACCAGTACA TTTTTTCACA CATTCTTCGT CTTGAGAAAC CTGCACTAGA ATTTAAACCC 
ACAGTAGCCG ATTGTGCCTA CTGTGTTCTA CCTCTTAACG TTGTAAATGA TTCTGGCACT 
TTGGACATTG ACTTCAAATT TGTAGAAGAT ATTGAGAAAT CAGAAGCACG TACTGGTATA 
CCTAATACAC AGTATTCAGC TGAAAGTCCT TTTATTTTCA AATTAGAAGA CTACCAGGAT 
GCTGTTATCA TTCCAAGGCA AGTAATATAT CGAAATTTTG ATCAGCCGCA CAGATTTTAT 
GTAGCTGATG TATACACTGA TCTTACACCG CTCAGCAAGT TCCCTTCCCC TGAATATGAA 
ACCTTTGCTG AATATTACAA AACAAAGTAT AACCTCGATC TCACAAACCT CAATCAGCCA 
CTTTTGGATG TGGACCACAC ATCATCAAGA CTTAATTTGC TGACTCCTCG CCATCTGAAT 
CAGAAAGGTA AAGCTCTGCC ATTAAGCAGT GCTGAAAAGA GAAAAGCCAA ATGGGAGAGT 
TTACAAAACA AACAGATCCT GGTTCCAGAG CTTTGTGCTA TACATCCAGT CCCAGCATCC 
TTATGGAGAA AAGCTGTTTG TTTGCCAAGC ATACTTTATC GATTACATTG CCTCCTTACA 
GCAGAAGAGC TGAGAGCGCA AACAGCTATT GATGCTGGGG TAGGAGTCAA ATCGCTTCCT 
GATGATTTCC GATACCCAAA CTTGGATTTT GGATGGAAAC GATCTATAGA CAGTAAAACA 
TTCATCTCTA ATCAAAGTTC CTCATCAGTC GAGAGTGAAA GTGACTGCAG ACTCAATAAA 
ACCACGGCCC CTGACAGTGC TGCAAGCTCA GCTGCTAATT CTGTAATCTA CATGCAAATC 
AATGACCAAA TGTCTGTGAA CTGCACACCA CCATGTCAAA AATCTCTGAG TCACCTCCAA 
ACAGTTTGCT TCTCAGATGA TTACAAAGCA ATTAACGGTA TTTCTTGCAA TGGTCTTACC 
AATGGTGATT GGGAAGCAGA AAGCGCTGCA TGTTTCCAAA AGGACGAACG GATAACCTGC 
AAACAGGAAA TACCTGAAAA ATCTACCTCG TTTCACGTTC AGAATTTACC TAAGGAGAAC 
CAGCCCATAC TTAAAGAATG TACTCTGAGC AACTCTGATG GAAATGTCAG CAAACCTACC 
TCAGATGAGT GCCCTAGCAC CTGTACTTCA GACATGCATT ATGATAGTGG GCTTTCCAAT 
AGGCACTCTT CAAAAACTCT TGGCCCAAAC CCTGGTCTTA TCCTTCAGGC CTTGACTCTG 
TCCAATGCAA GTGATGGTTT CAATCTGGAG CGTCTTGAAA TGCTTGGGGA CTCTTTCTTA 
AAACATGCTA TCACCACATA TCTGTTCTGC ACATACCCAG ATGCTCACGA GGGCCGTCTA 
TCCTATATGC GCAGCAAGAA GGTCAGTAAC TGTAACCTTT ATCGGCTTGG AAAAAAGAAA 
GGCTCACCCA GCCGGATGGT GGTGTCCATT TTCGATCCAC CTGTCAACTG GCTTCCCCCT 
GGTTATATTG TTAACCAGGA CAAAAACTCT GATAAGTGGG AAAGTAATGA AACGTCTGGT 
GAGGATGTGA TGGTTAATGG CAAAATTGAT GAAGACTTTG ATGATGAAGA AGATGAAGAT 
CTTATGTGGA GGAATCCCAA AGAGGAAACT GATTTTGATG ATGACTTTTT GGAATATGAT 
CAAGAGCATA TAAAATTCAT TGACAGCATG TTAATGGGAT CTGGGGCATT TGTGAAGAAA 
ATTCCTCTTT CTTCATTTGC ACCCCCTGAT CAGAACTATG AATGGAGGGC GCCAAAGAAA 
CCTCCACTGG AAAGTTCTCA ATTTCCCTGC GATTTTGATG ATTTTGATTA CAGTTCTTGG 
GATGCCATGT GTTATTTGGA TCCCAGCAAG GCTGTAGAGG AGGATGACTT TGTAGTAGGC 
TTTTGGAATC CATCAGAAGA GAACTGTGGA GCTGATGCTG GAAAGCAGTC TATATCTTAT 
GACCTACACA CAGAGCAGTG TATTGCAGAC AAAAGTATAG CTGACTGTGT AGAGGCTTTA 
TTAGGCTGCT ATCTGACCAG TTGTGGTGAG AGAGCTGCAC AACTGTTTCT TTGTTCTTTG 
GGCTTAAAAG TGCTCCCAGA AGTAAGAAAG CTTGTTACAA ACACAAATGT CATTAGTGCA 
TCATCATCTT ACCAGAACAG TACCAGAGAC AACTGCACTC TTACTGCCAG AACCAACACT 
GATCTGTCCT CCTGCAAAGG CATAGACTAC GGTTATTTAA AGATTCCCCC AAGGTGTATG 
TTTGAGCACC CAGATGCTGA GAAAACCCTT GACCATCTAA TATCTGGTTT TGAAAACTTT 
GAGAAGAAAA TAAACTATCC ATTTAAAAAC AAGGCTTACC TTCTACAGGC TTTTACACAT 
GCCTCTTACC ACTACAATAC TATAACTGAC TGCTACCAGC GTTTAGAATT TCTTGGAGAT 
GCAATTTTGG ACTACCTCAT TACTAAGCAC CTTTATGAAG ATCCACGCCA GCACTCGCCA 
GGAGTCCTCA CTGACCTGCG CTCTGCTCTT GTGAATAACA CCATATTTGC ATCACTAGCT 
GTGAAATATG ACTACCATAA ATACTTCAAG GCTATCTCTC CTGAACTGTT CCATGTAATT 
GATGATTTTG TGCAATTTCA ACTTGAAAAA AATGAAATGC AAGGCATGGA TTCTGAGTTA 
CGTAGGTCTG AAGAAGACGA AGAGAAAGAA GAGGACATTG AAGTCCCAAA AGCTATGGGA 
GACATATTTG AGTCACTTGC AGGAGCTATT TACATGGACA GCGGCATGTC TTTGGAAACT 
GTATGGCATG TGTATTATCC TATGATGCAG CCATTAATAG AAAAATTCTC TGCTAATGTT 
CCTCGTTCCC CAGTGAGAGA ATTACTGGAA ATGGAGCCTG AAACTGCTAA ATTCAGTCCA 
GCCGAAAGAA CATACGATGG AAAAGTCAGA GTGACTGTGG AAGTTGTTGG AAAAGGAAAA 
TTTAAAGGAG TCGGAAGAAG TTACAGAATT GCCAAATCTG CAGCAGCCAG GAGAGCACTA 
AGAAGCCTCA AAGCAAATCA ATCTCAGGTC CCTAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dicer1 ( XM_012968074.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823528.1 Xenopus tropicalis dicer 1, ribonuclease type III (dicer1), transcript variant X1, mRNA.

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CloneID	OXa32251
Clone ID Related Accession (Same CDS sequence)	XM_012968074.2 , XM_012968075.2
Accession Version	XM_012968074.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCC TTCAGCTCAT GACTCCGGCC TCCTCTCCAA TGGGTCCCTT CTTTGGGCTA 
CCCTGGCAAC AGGAGGCTAT TCATGACAAC ATTTATACCC CACGGAAATA TCAGGTGGAA 
CTACTCGAAG CAGCATTGGA TCATAATACT ATAGTATGTT TGAATTCTGG CTCAGGGAAG 
ACATTTATTG CAGTACTACT CAGTAAAGAG CTGTCATACC AGATCCGTGG GGACTTCAGC 
AAAAATACTA AGAGGACTGT GTTTTTGGTC AATTCCGAGA AACAGGTTTC TCAACAAGTG 
TCTGCTGTCA GGACCCATAC AGACCTCAAG GTGGGAGAAT ATTCAGATCA AGAAAAAACA 
CAATGCTGGG CAAAAGAAAG ATGGTACCTA GAATTTGAAA CTCATCAGGT CTTGGTCATG 
ACCTGCCACA TCTTCCTGAA CGTTCTGAAA AGTGGGAACG TGTCATTGTC AAACATTAAT 
CTGTTAGTGT TTGATGAATG TCATCTTGCA ATTCAGGATC ACCCATATCG AGAAATCATG 
AAGATATGTG AGAGTTGCCA GCCATGCCCT CGAATCCTGG GTCTAACTGC TTCAATTTTA 
AATGGAAAAT GTGACCCTCG TGACCTAGAG GAAAAGATCC AGAAACTGGA GGAAATATTA 
AGGAGTAATG CAGAAACTGC AACTGATTTG GTTGTTTTAG ACAGGTATGC TTCCCAACCA 
TGTGAAATTG TATTGGACTG TGGGCCATAT ATTGACAAAA GTGGACTTTA TCAAAGACTT 
CTAAATGAAT TGGATGAAGC CCTTAACTTT CTCATTGACT GTAATATTTC TACACATTCT 
AAAGAGAGAG ATTCCACATT AATTTCAAAA CAGATTCTAT CAGACTGTCA AACTGTTCTT 
TTGGTCTTGG GACCATGGTG TGCTGATAAA GTTGCGGGGA TGATGGTGAG AGAGCTGCAG 
AAGTATATCA AACATGAGCA GGAGGAACTG CACAGAAAAT TCCTTTTGTT TACAGACACT 
ATCTTAAGGA AGATCCATGC TCTTTGTGAG GAACACTTCT CGCCAGCCTC TCTTGATATG 
AAGTTTGTCA CACCTAAAGT TATAAAACTG CTGGAAATTT TACGCAAATA CAAACCCTAC 
GAACGCCAGC AATTTGAAAG TGTTGAATGG TATAACAATA GAAACCAGGA TAATTATGTG 
TCTTGGAGTG ATTCAGAGGA TGATGACGAT GAAGATGAAG AAATTGAGGA GAAAGAGAAA 
ACTGAAACAA GCTTTCCATC CCCATTCACA AACATCCTGT GTGGCATCAT CTTCGTGGAA 
CGAAGATACA CAGCAGTAGT ATTAAACAGG TTGATTAAAG AAGCCGGGAA GCAAGATCCA 
GAGCTGGCCT ATATCAGTAG TAACTTTATT ACTGGGCACG GCATAGGAAA GAACCAGCCA 
CGCAATAAGC AGATGGAAGT TGAGTTTAGA AAGCAAGAAG AGGTGCTTCG TAAATTTCGT 
GCACACGAAA CCAACTTATT GATAGCTACT AGCATTGTTG AGGAAGGAGT GGACATACCA 
AAATGCAACT TGGTAGTTCG ATTTGATTTA CCTTCAGAGT ACAGATCCTA TGTACAGTCC 
AAAGGCAGAG CAAGAGCACC AATCTCAAAT TACATCATGC TAGCCGATAG TGATAAAATT 
AAGGCATTTG AAGAGGACCT TAAAACATAC AAAGCAATTG AAAAGATTCT GCGGAACAAA 
TGCTCAAAGT CCATTGATTG TGGAAATACA GAATCTGAGC CCATTGTGGA CGATGATGAA 
ATATTTCCAC CATATGTGTT GAGACAGGAT GATGGCAGCC CACGAGTTAC TATCAACACC 
GCTATTGGAC ACATTAACAG GTACTGTGCT AGGCTACCTA GTGACCCATT TACTCATCTT 
GCTCCTAAGT GCAAAACACG AGAGTTCCCT GATGGACTTT ATCGCTCAAC ACTCTACCTG 
CCAATTAATT CTCCACTTAG AGCCCCCATT GTTGGCCCTC CGATGAATTG TGGAAGGCTA 
GCTGATAGAG CTGTAGCTCT TATATGCTGT AAAAAACTAC ATGAAATTGG TGAACTGGAT 
GATCATTTAA TGCCAGTTGG CAAGGAAACT GTAAAATATG AGGAGGAGCT TGATTTGCAT 
GATGAGGAAG AAACCAGTGT TCCAGGCAGA CCAGGATCCA CAAAAAGAAG GCAGTGTTAT 
CCAAAAGCTA TTCCTGAATG TTTACGGAAC AGCTACCCCA AGCCTGGTCA GCCTTGTTAC 
TTATATGTAA TAGGAATGGT ATTAACCACT CCTCTACCAG ATGAACTTAA TTTTAGGCGA 
CGGAAGCTGT ATCCCCCTGA AGACACAACA AGATGCTTTG GAATACTAAC TGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTCTAT ACTCGTTCGG GAGAGGTGAC CATATCAATT 
GAACTAAAGA AGTCTGGTTT TACATTAAAC TTAGAACAAC TTGAGCTCAT TACTAGACTC 
CACCAGTACA TTTTTTCACA CATTCTTCGT CTTGAGAAAC CTGCACTAGA ATTTAAACCC 
ACAGTAGCCG ATTGTGCCTA CTGTGTTCTA CCTCTTAACG TTGTAAATGA TTCTGGCACT 
TTGGACATTG ACTTCAAATT TGTAGAAGAT ATTGAGAAAT CAGAAGCACG TACTGGTATA 
CCTAATACAC AGTATTCAGC TGAAAGTCCT TTTATTTTCA AATTAGAAGA CTACCAGGAT 
GCTGTTATCA TTCCAAGATA TCGAAATTTT GATCAGCCGC ACAGATTTTA TGTAGCTGAT 
GTATACACTG ATCTTACACC GCTCAGCAAG TTCCCTTCCC CTGAATATGA AACCTTTGCT 
GAATATTACA AAACAAAGTA TAACCTCGAT CTCACAAACC TCAATCAGCC ACTTTTGGAT 
GTGGACCACA CATCATCAAG ACTTAATTTG CTGACTCCTC GCCATCTGAA TCAGAAAGGT 
AAAGCTCTGC CATTAAGCAG TGCTGAAAAG AGAAAAGCCA AATGGGAGAG TTTACAAAAC 
AAACAGATCC TGGTTCCAGA GCTTTGTGCT ATACATCCAG TCCCAGCATC CTTATGGAGA 
AAAGCTGTTT GTTTGCCAAG CATACTTTAT CGATTACATT GCCTCCTTAC AGCAGAAGAG 
CTGAGAGCGC AAACAGCTAT TGATGCTGGG GTAGGAGTCA AATCGCTTCC TGATGATTTC 
CGATACCCAA ACTTGGATTT TGGATGGAAA CGATCTATAG ACAGTAAAAC ATTCATCTCT 
AATCAAAGTT CCTCATCAGT CGAGAGTGAA AGTGACTGCA GACTCAATAA AACCACGGCC 
CCTGACAGTG CTGCAAGCTC AGCTGCTAAT TCTGTAATCT ACATGCAAAT CAATGACCAA 
ATGTCTGTGA ACTGCACACC ACCATGTCAA AAATCTCTGA GTCACCTCCA AACAGTTTGC 
TTCTCAGATG ATTACAAAGC AATTAACGGT ATTTCTTGCA ATGGTCTTAC CAATGGTGAT 
TGGGAAGCAG AAAGCGCTGC ATGTTTCCAA AAGGACGAAC GGATAACCTG CAAACAGGAA 
ATACCTGAAA AATCTACCTC GTTTCACGTT CAGAATTTAC CTAAGGAGAA CCAGCCCATA 
CTTAAAGAAT GTACTCTGAG CAACTCTGAT GGAAATGTCA GCAAACCTAC CTCAGATGAG 
TGCCCTAGCA CCTGTACTTC AGACATGCAT TATGATAGTG GGCTTTCCAA TAGGCACTCT 
TCAAAAACTC TTGGCCCAAA CCCTGGTCTT ATCCTTCAGG CCTTGACTCT GTCCAATGCA 
AGTGATGGTT TCAATCTGGA GCGTCTTGAA ATGCTTGGGG ACTCTTTCTT AAAACATGCT 
ATCACCACAT ATCTGTTCTG CACATACCCA GATGCTCACG AGGGCCGTCT ATCCTATATG 
CGCAGCAAGA AGGTCAGTAA CTGTAACCTT TATCGGCTTG GAAAAAAGAA AGGCTCACCC 
AGCCGGATGG TGGTGTCCAT TTTCGATCCA CCTGTCAACT GGCTTCCCCC TGGTTATATT 
GTTAACCAGG ACAAAAACTC TGATAAGTGG GAAAGTAATG AAACGTCTGG TGAGGATGTG 
ATGGTTAATG GCAAAATTGA TGAAGACTTT GATGATGAAG AAGATGAAGA TCTTATGTGG 
AGGAATCCCA AAGAGGAAAC TGATTTTGAT GATGACTTTT TGGAATATGA TCAAGAGCAT 
ATAAAATTCA TTGACAGCAT GTTAATGGGA TCTGGGGCAT TTGTGAAGAA AATTCCTCTT 
TCTTCATTTG CACCCCCTGA TCAGAACTAT GAATGGAGGG CGCCAAAGAA ACCTCCACTG 
GAAAGTTCTC AATTTCCCTG CGATTTTGAT GATTTTGATT ACAGTTCTTG GGATGCCATG 
TGTTATTTGG ATCCCAGCAA GGCTGTAGAG GAGGATGACT TTGTAGTAGG CTTTTGGAAT 
CCATCAGAAG AGAACTGTGG AGCTGATGCT GGAAAGCAGT CTATATCTTA TGACCTACAC 
ACAGAGCAGT GTATTGCAGA CAAAAGTATA GCTGACTGTG TAGAGGCTTT ATTAGGCTGC 
TATCTGACCA GTTGTGGTGA GAGAGCTGCA CAACTGTTTC TTTGTTCTTT GGGCTTAAAA 
GTGCTCCCAG AAGTAAGAAA GCTTGTTACA AACACAAATG TCATTAGTGC ATCATCATCT 
TACCAGAACA GTACCAGAGA CAACTGCACT CTTACTGCCA GAACCAACAC TGATCTGTCC 
TCCTGCAAAG GCATAGACTA CGGTTATTTA AAGATTCCCC CAAGGTGTAT GTTTGAGCAC 
CCAGATGCTG AGAAAACCCT TGACCATCTA ATATCTGGTT TTGAAAACTT TGAGAAGAAA 
ATAAACTATC CATTTAAAAA CAAGGCTTAC CTTCTACAGG CTTTTACACA TGCCTCTTAC 
CACTACAATA CTATAACTGA CTGCTACCAG CGTTTAGAAT TTCTTGGAGA TGCAATTTTG 
GACTACCTCA TTACTAAGCA CCTTTATGAA GATCCACGCC AGCACTCGCC AGGAGTCCTC 
ACTGACCTGC GCTCTGCTCT TGTGAATAAC ACCATATTTG CATCACTAGC TGTGAAATAT 
GACTACCATA AATACTTCAA GGCTATCTCT CCTGAACTGT TCCATGTAAT TGATGATTTT 
GTGCAATTTC AACTTGAAAA AAATGAAATG CAAGGCATGG ATTCTGAGTT ACGTAGGTCT 
GAAGAAGACG AAGAGAAAGA AGAGGACATT GAAGTCCCAA AAGCTATGGG AGACATATTT 
GAGTCACTTG CAGGAGCTAT TTACATGGAC AGCGGCATGT CTTTGGAAAC TGTATGGCAT 
GTGTATTATC CTATGATGCA GCCATTAATA GAAAAATTCT CTGCTAATGT TCCTCGTTCC 
CCAGTGAGAG AATTACTGGA AATGGAGCCT GAAACTGCTA AATTCAGTCC AGCCGAAAGA 
ACATACGATG GAAAAGTCAG AGTGACTGTG GAAGTTGTTG GAAAAGGAAA ATTTAAAGGA 
GTCGGAAGAA GTTACAGAAT TGCCAAATCT GCAGCAGCCA GGAGAGCACT AAGAAGCCTC 
AAAGCAAATC AATCTCAGGT CCCTAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823528.1
CDS	363..6035
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNSGSGKTFIAVLLSKELSYQIRGDFSKNTKRTVFLVNSEKQVSQQVSAVRTHTDLKVGEYSDQEKTQCWAKERWYLEFETHQVLVMTCHIFLNVLKSGNVSLSNINLLVFDECHLAIQDHPYREIMKICESCQPCPRILGLTASILNGKCDPRDLEEKIQKLEEILRSNAETATDLVVLDRYASQPCEIVLDCGPYIDKSGLYQRLLNELDEALNFLIDCNISTHSKERDSTLISKQILSDCQTVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTILRKIHALCEEHFSPASLDMKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDEDEEIEEKEKTETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPSEYRSYVQSKGRARAPISNYIMLADSDKIKAFEEDLKTYKAIEKILRNKCSKSIDCGNTESEPIVDDDEIFPPYVLRQDDGSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTREFPDGLYRSTLYLPINSPLRAPIVGPPMNCGRLADRAVALICCKKLHEIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLNLEQLELITRLHQYIFSHILRLEKPALEFKPTVADCAYCVLPLNVVNDSGTLDIDFKFVEDIEKSEARTGIPNTQYSAESPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPVPASLWRKAVCLPSILYRLHCLLTAEELRAQTAIDAGVGVKSLPDDFRYPNLDFGWKRSIDSKTFISNQSSSSVESESDCRLNKTTAPDSAASSAANSVIYMQINDQMSVNCTPPCQKSLSHLQTVCFSDDYKAINGISCNGLTNGDWEAESAACFQKDERITCKQEIPEKSTSFHVQNLPKENQPILKECTLSNSDGNVSKPTSDECPSTCTSDMHYDSGLSNRHSSKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGSPSRMVVSIFDPPVNWLPPGYIVNQDKNSDKWESNETSGEDVMVNGKIDEDFDDEEDEDLMWRNPKEETDFDDDFLEYDQEHIKFIDSMLMGSGAFVKKIPLSSFAPPDQNYEWRAPKKPPLESSQFPCDFDDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGADAGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPEVRKLVTNTNVISASSSYQNSTRDNCTLTARTNTDLSSCKGIDYGYLKIPPRCMFEHPDAEKTLDHLISGFENFEKKINYPFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWHVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQSQVPNS

Target ORF information:

RefSeq Version	XM_012968074.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis dicer 1, ribonuclease type III (dicer1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968074.2

ORF Insert Sequence:

ATGGCAGGCC TTCAGCTCAT GACTCCGGCC TCCTCTCCAA TGGGTCCCTT CTTTGGGCTA 
CCCTGGCAAC AGGAGGCTAT TCATGACAAC ATTTATACCC CACGGAAATA TCAGGTGGAA 
CTACTCGAAG CAGCATTGGA TCATAATACT ATAGTATGTT TGAATTCTGG CTCAGGGAAG 
ACATTTATTG CAGTACTACT CAGTAAAGAG CTGTCATACC AGATCCGTGG GGACTTCAGC 
AAAAATACTA AGAGGACTGT GTTTTTGGTC AATTCCGAGA AACAGGTTTC TCAACAAGTG 
TCTGCTGTCA GGACCCATAC AGACCTCAAG GTGGGAGAAT ATTCAGATCA AGAAAAAACA 
CAATGCTGGG CAAAAGAAAG ATGGTACCTA GAATTTGAAA CTCATCAGGT CTTGGTCATG 
ACCTGCCACA TCTTCCTGAA CGTTCTGAAA AGTGGGAACG TGTCATTGTC AAACATTAAT 
CTGTTAGTGT TTGATGAATG TCATCTTGCA ATTCAGGATC ACCCATATCG AGAAATCATG 
AAGATATGTG AGAGTTGCCA GCCATGCCCT CGAATCCTGG GTCTAACTGC TTCAATTTTA 
AATGGAAAAT GTGACCCTCG TGACCTAGAG GAAAAGATCC AGAAACTGGA GGAAATATTA 
AGGAGTAATG CAGAAACTGC AACTGATTTG GTTGTTTTAG ACAGGTATGC TTCCCAACCA 
TGTGAAATTG TATTGGACTG TGGGCCATAT ATTGACAAAA GTGGACTTTA TCAAAGACTT 
CTAAATGAAT TGGATGAAGC CCTTAACTTT CTCATTGACT GTAATATTTC TACACATTCT 
AAAGAGAGAG ATTCCACATT AATTTCAAAA CAGATTCTAT CAGACTGTCA AACTGTTCTT 
TTGGTCTTGG GACCATGGTG TGCTGATAAA GTTGCGGGGA TGATGGTGAG AGAGCTGCAG 
AAGTATATCA AACATGAGCA GGAGGAACTG CACAGAAAAT TCCTTTTGTT TACAGACACT 
ATCTTAAGGA AGATCCATGC TCTTTGTGAG GAACACTTCT CGCCAGCCTC TCTTGATATG 
AAGTTTGTCA CACCTAAAGT TATAAAACTG CTGGAAATTT TACGCAAATA CAAACCCTAC 
GAACGCCAGC AATTTGAAAG TGTTGAATGG TATAACAATA GAAACCAGGA TAATTATGTG 
TCTTGGAGTG ATTCAGAGGA TGATGACGAT GAAGATGAAG AAATTGAGGA GAAAGAGAAA 
ACTGAAACAA GCTTTCCATC CCCATTCACA AACATCCTGT GTGGCATCAT CTTCGTGGAA 
CGAAGATACA CAGCAGTAGT ATTAAACAGG TTGATTAAAG AAGCCGGGAA GCAAGATCCA 
GAGCTGGCCT ATATCAGTAG TAACTTTATT ACTGGGCACG GCATAGGAAA GAACCAGCCA 
CGCAATAAGC AGATGGAAGT TGAGTTTAGA AAGCAAGAAG AGGTGCTTCG TAAATTTCGT 
GCACACGAAA CCAACTTATT GATAGCTACT AGCATTGTTG AGGAAGGAGT GGACATACCA 
AAATGCAACT TGGTAGTTCG ATTTGATTTA CCTTCAGAGT ACAGATCCTA TGTACAGTCC 
AAAGGCAGAG CAAGAGCACC AATCTCAAAT TACATCATGC TAGCCGATAG TGATAAAATT 
AAGGCATTTG AAGAGGACCT TAAAACATAC AAAGCAATTG AAAAGATTCT GCGGAACAAA 
TGCTCAAAGT CCATTGATTG TGGAAATACA GAATCTGAGC CCATTGTGGA CGATGATGAA 
ATATTTCCAC CATATGTGTT GAGACAGGAT GATGGCAGCC CACGAGTTAC TATCAACACC 
GCTATTGGAC ACATTAACAG GTACTGTGCT AGGCTACCTA GTGACCCATT TACTCATCTT 
GCTCCTAAGT GCAAAACACG AGAGTTCCCT GATGGACTTT ATCGCTCAAC ACTCTACCTG 
CCAATTAATT CTCCACTTAG AGCCCCCATT GTTGGCCCTC CGATGAATTG TGGAAGGCTA 
GCTGATAGAG CTGTAGCTCT TATATGCTGT AAAAAACTAC ATGAAATTGG TGAACTGGAT 
GATCATTTAA TGCCAGTTGG CAAGGAAACT GTAAAATATG AGGAGGAGCT TGATTTGCAT 
GATGAGGAAG AAACCAGTGT TCCAGGCAGA CCAGGATCCA CAAAAAGAAG GCAGTGTTAT 
CCAAAAGCTA TTCCTGAATG TTTACGGAAC AGCTACCCCA AGCCTGGTCA GCCTTGTTAC 
TTATATGTAA TAGGAATGGT ATTAACCACT CCTCTACCAG ATGAACTTAA TTTTAGGCGA 
CGGAAGCTGT ATCCCCCTGA AGACACAACA AGATGCTTTG GAATACTAAC TGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTCTAT ACTCGTTCGG GAGAGGTGAC CATATCAATT 
GAACTAAAGA AGTCTGGTTT TACATTAAAC TTAGAACAAC TTGAGCTCAT TACTAGACTC 
CACCAGTACA TTTTTTCACA CATTCTTCGT CTTGAGAAAC CTGCACTAGA ATTTAAACCC 
ACAGTAGCCG ATTGTGCCTA CTGTGTTCTA CCTCTTAACG TTGTAAATGA TTCTGGCACT 
TTGGACATTG ACTTCAAATT TGTAGAAGAT ATTGAGAAAT CAGAAGCACG TACTGGTATA 
CCTAATACAC AGTATTCAGC TGAAAGTCCT TTTATTTTCA AATTAGAAGA CTACCAGGAT 
GCTGTTATCA TTCCAAGATA TCGAAATTTT GATCAGCCGC ACAGATTTTA TGTAGCTGAT 
GTATACACTG ATCTTACACC GCTCAGCAAG TTCCCTTCCC CTGAATATGA AACCTTTGCT 
GAATATTACA AAACAAAGTA TAACCTCGAT CTCACAAACC TCAATCAGCC ACTTTTGGAT 
GTGGACCACA CATCATCAAG ACTTAATTTG CTGACTCCTC GCCATCTGAA TCAGAAAGGT 
AAAGCTCTGC CATTAAGCAG TGCTGAAAAG AGAAAAGCCA AATGGGAGAG TTTACAAAAC 
AAACAGATCC TGGTTCCAGA GCTTTGTGCT ATACATCCAG TCCCAGCATC CTTATGGAGA 
AAAGCTGTTT GTTTGCCAAG CATACTTTAT CGATTACATT GCCTCCTTAC AGCAGAAGAG 
CTGAGAGCGC AAACAGCTAT TGATGCTGGG GTAGGAGTCA AATCGCTTCC TGATGATTTC 
CGATACCCAA ACTTGGATTT TGGATGGAAA CGATCTATAG ACAGTAAAAC ATTCATCTCT 
AATCAAAGTT CCTCATCAGT CGAGAGTGAA AGTGACTGCA GACTCAATAA AACCACGGCC 
CCTGACAGTG CTGCAAGCTC AGCTGCTAAT TCTGTAATCT ACATGCAAAT CAATGACCAA 
ATGTCTGTGA ACTGCACACC ACCATGTCAA AAATCTCTGA GTCACCTCCA AACAGTTTGC 
TTCTCAGATG ATTACAAAGC AATTAACGGT ATTTCTTGCA ATGGTCTTAC CAATGGTGAT 
TGGGAAGCAG AAAGCGCTGC ATGTTTCCAA AAGGACGAAC GGATAACCTG CAAACAGGAA 
ATACCTGAAA AATCTACCTC GTTTCACGTT CAGAATTTAC CTAAGGAGAA CCAGCCCATA 
CTTAAAGAAT GTACTCTGAG CAACTCTGAT GGAAATGTCA GCAAACCTAC CTCAGATGAG 
TGCCCTAGCA CCTGTACTTC AGACATGCAT TATGATAGTG GGCTTTCCAA TAGGCACTCT 
TCAAAAACTC TTGGCCCAAA CCCTGGTCTT ATCCTTCAGG CCTTGACTCT GTCCAATGCA 
AGTGATGGTT TCAATCTGGA GCGTCTTGAA ATGCTTGGGG ACTCTTTCTT AAAACATGCT 
ATCACCACAT ATCTGTTCTG CACATACCCA GATGCTCACG AGGGCCGTCT ATCCTATATG 
CGCAGCAAGA AGGTCAGTAA CTGTAACCTT TATCGGCTTG GAAAAAAGAA AGGCTCACCC 
AGCCGGATGG TGGTGTCCAT TTTCGATCCA CCTGTCAACT GGCTTCCCCC TGGTTATATT 
GTTAACCAGG ACAAAAACTC TGATAAGTGG GAAAGTAATG AAACGTCTGG TGAGGATGTG 
ATGGTTAATG GCAAAATTGA TGAAGACTTT GATGATGAAG AAGATGAAGA TCTTATGTGG 
AGGAATCCCA AAGAGGAAAC TGATTTTGAT GATGACTTTT TGGAATATGA TCAAGAGCAT 
ATAAAATTCA TTGACAGCAT GTTAATGGGA TCTGGGGCAT TTGTGAAGAA AATTCCTCTT 
TCTTCATTTG CACCCCCTGA TCAGAACTAT GAATGGAGGG CGCCAAAGAA ACCTCCACTG 
GAAAGTTCTC AATTTCCCTG CGATTTTGAT GATTTTGATT ACAGTTCTTG GGATGCCATG 
TGTTATTTGG ATCCCAGCAA GGCTGTAGAG GAGGATGACT TTGTAGTAGG CTTTTGGAAT 
CCATCAGAAG AGAACTGTGG AGCTGATGCT GGAAAGCAGT CTATATCTTA TGACCTACAC 
ACAGAGCAGT GTATTGCAGA CAAAAGTATA GCTGACTGTG TAGAGGCTTT ATTAGGCTGC 
TATCTGACCA GTTGTGGTGA GAGAGCTGCA CAACTGTTTC TTTGTTCTTT GGGCTTAAAA 
GTGCTCCCAG AAGTAAGAAA GCTTGTTACA AACACAAATG TCATTAGTGC ATCATCATCT 
TACCAGAACA GTACCAGAGA CAACTGCACT CTTACTGCCA GAACCAACAC TGATCTGTCC 
TCCTGCAAAG GCATAGACTA CGGTTATTTA AAGATTCCCC CAAGGTGTAT GTTTGAGCAC 
CCAGATGCTG AGAAAACCCT TGACCATCTA ATATCTGGTT TTGAAAACTT TGAGAAGAAA 
ATAAACTATC CATTTAAAAA CAAGGCTTAC CTTCTACAGG CTTTTACACA TGCCTCTTAC 
CACTACAATA CTATAACTGA CTGCTACCAG CGTTTAGAAT TTCTTGGAGA TGCAATTTTG 
GACTACCTCA TTACTAAGCA CCTTTATGAA GATCCACGCC AGCACTCGCC AGGAGTCCTC 
ACTGACCTGC GCTCTGCTCT TGTGAATAAC ACCATATTTG CATCACTAGC TGTGAAATAT 
GACTACCATA AATACTTCAA GGCTATCTCT CCTGAACTGT TCCATGTAAT TGATGATTTT 
GTGCAATTTC AACTTGAAAA AAATGAAATG CAAGGCATGG ATTCTGAGTT ACGTAGGTCT 
GAAGAAGACG AAGAGAAAGA AGAGGACATT GAAGTCCCAA AAGCTATGGG AGACATATTT 
GAGTCACTTG CAGGAGCTAT TTACATGGAC AGCGGCATGT CTTTGGAAAC TGTATGGCAT 
GTGTATTATC CTATGATGCA GCCATTAATA GAAAAATTCT CTGCTAATGT TCCTCGTTCC 
CCAGTGAGAG AATTACTGGA AATGGAGCCT GAAACTGCTA AATTCAGTCC AGCCGAAAGA 
ACATACGATG GAAAAGTCAG AGTGACTGTG GAAGTTGTTG GAAAAGGAAA ATTTAAAGGA 
GTCGGAAGAA GTTACAGAAT TGCCAAATCT GCAGCAGCCA GGAGAGCACT AAGAAGCCTC 
AAAGCAAATC AATCTCAGGT CCCTAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dicer1 ( XM_012968075.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823529.1 Xenopus tropicalis dicer 1, ribonuclease type III (dicer1), transcript variant X2, mRNA.

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CloneID	OXa32251
Clone ID Related Accession (Same CDS sequence)	XM_012968074.2 , XM_012968075.2
Accession Version	XM_012968075.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCC TTCAGCTCAT GACTCCGGCC TCCTCTCCAA TGGGTCCCTT CTTTGGGCTA 
CCCTGGCAAC AGGAGGCTAT TCATGACAAC ATTTATACCC CACGGAAATA TCAGGTGGAA 
CTACTCGAAG CAGCATTGGA TCATAATACT ATAGTATGTT TGAATTCTGG CTCAGGGAAG 
ACATTTATTG CAGTACTACT CAGTAAAGAG CTGTCATACC AGATCCGTGG GGACTTCAGC 
AAAAATACTA AGAGGACTGT GTTTTTGGTC AATTCCGAGA AACAGGTTTC TCAACAAGTG 
TCTGCTGTCA GGACCCATAC AGACCTCAAG GTGGGAGAAT ATTCAGATCA AGAAAAAACA 
CAATGCTGGG CAAAAGAAAG ATGGTACCTA GAATTTGAAA CTCATCAGGT CTTGGTCATG 
ACCTGCCACA TCTTCCTGAA CGTTCTGAAA AGTGGGAACG TGTCATTGTC AAACATTAAT 
CTGTTAGTGT TTGATGAATG TCATCTTGCA ATTCAGGATC ACCCATATCG AGAAATCATG 
AAGATATGTG AGAGTTGCCA GCCATGCCCT CGAATCCTGG GTCTAACTGC TTCAATTTTA 
AATGGAAAAT GTGACCCTCG TGACCTAGAG GAAAAGATCC AGAAACTGGA GGAAATATTA 
AGGAGTAATG CAGAAACTGC AACTGATTTG GTTGTTTTAG ACAGGTATGC TTCCCAACCA 
TGTGAAATTG TATTGGACTG TGGGCCATAT ATTGACAAAA GTGGACTTTA TCAAAGACTT 
CTAAATGAAT TGGATGAAGC CCTTAACTTT CTCATTGACT GTAATATTTC TACACATTCT 
AAAGAGAGAG ATTCCACATT AATTTCAAAA CAGATTCTAT CAGACTGTCA AACTGTTCTT 
TTGGTCTTGG GACCATGGTG TGCTGATAAA GTTGCGGGGA TGATGGTGAG AGAGCTGCAG 
AAGTATATCA AACATGAGCA GGAGGAACTG CACAGAAAAT TCCTTTTGTT TACAGACACT 
ATCTTAAGGA AGATCCATGC TCTTTGTGAG GAACACTTCT CGCCAGCCTC TCTTGATATG 
AAGTTTGTCA CACCTAAAGT TATAAAACTG CTGGAAATTT TACGCAAATA CAAACCCTAC 
GAACGCCAGC AATTTGAAAG TGTTGAATGG TATAACAATA GAAACCAGGA TAATTATGTG 
TCTTGGAGTG ATTCAGAGGA TGATGACGAT GAAGATGAAG AAATTGAGGA GAAAGAGAAA 
ACTGAAACAA GCTTTCCATC CCCATTCACA AACATCCTGT GTGGCATCAT CTTCGTGGAA 
CGAAGATACA CAGCAGTAGT ATTAAACAGG TTGATTAAAG AAGCCGGGAA GCAAGATCCA 
GAGCTGGCCT ATATCAGTAG TAACTTTATT ACTGGGCACG GCATAGGAAA GAACCAGCCA 
CGCAATAAGC AGATGGAAGT TGAGTTTAGA AAGCAAGAAG AGGTGCTTCG TAAATTTCGT 
GCACACGAAA CCAACTTATT GATAGCTACT AGCATTGTTG AGGAAGGAGT GGACATACCA 
AAATGCAACT TGGTAGTTCG ATTTGATTTA CCTTCAGAGT ACAGATCCTA TGTACAGTCC 
AAAGGCAGAG CAAGAGCACC AATCTCAAAT TACATCATGC TAGCCGATAG TGATAAAATT 
AAGGCATTTG AAGAGGACCT TAAAACATAC AAAGCAATTG AAAAGATTCT GCGGAACAAA 
TGCTCAAAGT CCATTGATTG TGGAAATACA GAATCTGAGC CCATTGTGGA CGATGATGAA 
ATATTTCCAC CATATGTGTT GAGACAGGAT GATGGCAGCC CACGAGTTAC TATCAACACC 
GCTATTGGAC ACATTAACAG GTACTGTGCT AGGCTACCTA GTGACCCATT TACTCATCTT 
GCTCCTAAGT GCAAAACACG AGAGTTCCCT GATGGACTTT ATCGCTCAAC ACTCTACCTG 
CCAATTAATT CTCCACTTAG AGCCCCCATT GTTGGCCCTC CGATGAATTG TGGAAGGCTA 
GCTGATAGAG CTGTAGCTCT TATATGCTGT AAAAAACTAC ATGAAATTGG TGAACTGGAT 
GATCATTTAA TGCCAGTTGG CAAGGAAACT GTAAAATATG AGGAGGAGCT TGATTTGCAT 
GATGAGGAAG AAACCAGTGT TCCAGGCAGA CCAGGATCCA CAAAAAGAAG GCAGTGTTAT 
CCAAAAGCTA TTCCTGAATG TTTACGGAAC AGCTACCCCA AGCCTGGTCA GCCTTGTTAC 
TTATATGTAA TAGGAATGGT ATTAACCACT CCTCTACCAG ATGAACTTAA TTTTAGGCGA 
CGGAAGCTGT ATCCCCCTGA AGACACAACA AGATGCTTTG GAATACTAAC TGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTCTAT ACTCGTTCGG GAGAGGTGAC CATATCAATT 
GAACTAAAGA AGTCTGGTTT TACATTAAAC TTAGAACAAC TTGAGCTCAT TACTAGACTC 
CACCAGTACA TTTTTTCACA CATTCTTCGT CTTGAGAAAC CTGCACTAGA ATTTAAACCC 
ACAGTAGCCG ATTGTGCCTA CTGTGTTCTA CCTCTTAACG TTGTAAATGA TTCTGGCACT 
TTGGACATTG ACTTCAAATT TGTAGAAGAT ATTGAGAAAT CAGAAGCACG TACTGGTATA 
CCTAATACAC AGTATTCAGC TGAAAGTCCT TTTATTTTCA AATTAGAAGA CTACCAGGAT 
GCTGTTATCA TTCCAAGATA TCGAAATTTT GATCAGCCGC ACAGATTTTA TGTAGCTGAT 
GTATACACTG ATCTTACACC GCTCAGCAAG TTCCCTTCCC CTGAATATGA AACCTTTGCT 
GAATATTACA AAACAAAGTA TAACCTCGAT CTCACAAACC TCAATCAGCC ACTTTTGGAT 
GTGGACCACA CATCATCAAG ACTTAATTTG CTGACTCCTC GCCATCTGAA TCAGAAAGGT 
AAAGCTCTGC CATTAAGCAG TGCTGAAAAG AGAAAAGCCA AATGGGAGAG TTTACAAAAC 
AAACAGATCC TGGTTCCAGA GCTTTGTGCT ATACATCCAG TCCCAGCATC CTTATGGAGA 
AAAGCTGTTT GTTTGCCAAG CATACTTTAT CGATTACATT GCCTCCTTAC AGCAGAAGAG 
CTGAGAGCGC AAACAGCTAT TGATGCTGGG GTAGGAGTCA AATCGCTTCC TGATGATTTC 
CGATACCCAA ACTTGGATTT TGGATGGAAA CGATCTATAG ACAGTAAAAC ATTCATCTCT 
AATCAAAGTT CCTCATCAGT CGAGAGTGAA AGTGACTGCA GACTCAATAA AACCACGGCC 
CCTGACAGTG CTGCAAGCTC AGCTGCTAAT TCTGTAATCT ACATGCAAAT CAATGACCAA 
ATGTCTGTGA ACTGCACACC ACCATGTCAA AAATCTCTGA GTCACCTCCA AACAGTTTGC 
TTCTCAGATG ATTACAAAGC AATTAACGGT ATTTCTTGCA ATGGTCTTAC CAATGGTGAT 
TGGGAAGCAG AAAGCGCTGC ATGTTTCCAA AAGGACGAAC GGATAACCTG CAAACAGGAA 
ATACCTGAAA AATCTACCTC GTTTCACGTT CAGAATTTAC CTAAGGAGAA CCAGCCCATA 
CTTAAAGAAT GTACTCTGAG CAACTCTGAT GGAAATGTCA GCAAACCTAC CTCAGATGAG 
TGCCCTAGCA CCTGTACTTC AGACATGCAT TATGATAGTG GGCTTTCCAA TAGGCACTCT 
TCAAAAACTC TTGGCCCAAA CCCTGGTCTT ATCCTTCAGG CCTTGACTCT GTCCAATGCA 
AGTGATGGTT TCAATCTGGA GCGTCTTGAA ATGCTTGGGG ACTCTTTCTT AAAACATGCT 
ATCACCACAT ATCTGTTCTG CACATACCCA GATGCTCACG AGGGCCGTCT ATCCTATATG 
CGCAGCAAGA AGGTCAGTAA CTGTAACCTT TATCGGCTTG GAAAAAAGAA AGGCTCACCC 
AGCCGGATGG TGGTGTCCAT TTTCGATCCA CCTGTCAACT GGCTTCCCCC TGGTTATATT 
GTTAACCAGG ACAAAAACTC TGATAAGTGG GAAAGTAATG AAACGTCTGG TGAGGATGTG 
ATGGTTAATG GCAAAATTGA TGAAGACTTT GATGATGAAG AAGATGAAGA TCTTATGTGG 
AGGAATCCCA AAGAGGAAAC TGATTTTGAT GATGACTTTT TGGAATATGA TCAAGAGCAT 
ATAAAATTCA TTGACAGCAT GTTAATGGGA TCTGGGGCAT TTGTGAAGAA AATTCCTCTT 
TCTTCATTTG CACCCCCTGA TCAGAACTAT GAATGGAGGG CGCCAAAGAA ACCTCCACTG 
GAAAGTTCTC AATTTCCCTG CGATTTTGAT GATTTTGATT ACAGTTCTTG GGATGCCATG 
TGTTATTTGG ATCCCAGCAA GGCTGTAGAG GAGGATGACT TTGTAGTAGG CTTTTGGAAT 
CCATCAGAAG AGAACTGTGG AGCTGATGCT GGAAAGCAGT CTATATCTTA TGACCTACAC 
ACAGAGCAGT GTATTGCAGA CAAAAGTATA GCTGACTGTG TAGAGGCTTT ATTAGGCTGC 
TATCTGACCA GTTGTGGTGA GAGAGCTGCA CAACTGTTTC TTTGTTCTTT GGGCTTAAAA 
GTGCTCCCAG AAGTAAGAAA GCTTGTTACA AACACAAATG TCATTAGTGC ATCATCATCT 
TACCAGAACA GTACCAGAGA CAACTGCACT CTTACTGCCA GAACCAACAC TGATCTGTCC 
TCCTGCAAAG GCATAGACTA CGGTTATTTA AAGATTCCCC CAAGGTGTAT GTTTGAGCAC 
CCAGATGCTG AGAAAACCCT TGACCATCTA ATATCTGGTT TTGAAAACTT TGAGAAGAAA 
ATAAACTATC CATTTAAAAA CAAGGCTTAC CTTCTACAGG CTTTTACACA TGCCTCTTAC 
CACTACAATA CTATAACTGA CTGCTACCAG CGTTTAGAAT TTCTTGGAGA TGCAATTTTG 
GACTACCTCA TTACTAAGCA CCTTTATGAA GATCCACGCC AGCACTCGCC AGGAGTCCTC 
ACTGACCTGC GCTCTGCTCT TGTGAATAAC ACCATATTTG CATCACTAGC TGTGAAATAT 
GACTACCATA AATACTTCAA GGCTATCTCT CCTGAACTGT TCCATGTAAT TGATGATTTT 
GTGCAATTTC AACTTGAAAA AAATGAAATG CAAGGCATGG ATTCTGAGTT ACGTAGGTCT 
GAAGAAGACG AAGAGAAAGA AGAGGACATT GAAGTCCCAA AAGCTATGGG AGACATATTT 
GAGTCACTTG CAGGAGCTAT TTACATGGAC AGCGGCATGT CTTTGGAAAC TGTATGGCAT 
GTGTATTATC CTATGATGCA GCCATTAATA GAAAAATTCT CTGCTAATGT TCCTCGTTCC 
CCAGTGAGAG AATTACTGGA AATGGAGCCT GAAACTGCTA AATTCAGTCC AGCCGAAAGA 
ACATACGATG GAAAAGTCAG AGTGACTGTG GAAGTTGTTG GAAAAGGAAA ATTTAAAGGA 
GTCGGAAGAA GTTACAGAAT TGCCAAATCT GCAGCAGCCA GGAGAGCACT AAGAAGCCTC 
AAAGCAAATC AATCTCAGGT CCCTAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823529.1
CDS	277..5949
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNSGSGKTFIAVLLSKELSYQIRGDFSKNTKRTVFLVNSEKQVSQQVSAVRTHTDLKVGEYSDQEKTQCWAKERWYLEFETHQVLVMTCHIFLNVLKSGNVSLSNINLLVFDECHLAIQDHPYREIMKICESCQPCPRILGLTASILNGKCDPRDLEEKIQKLEEILRSNAETATDLVVLDRYASQPCEIVLDCGPYIDKSGLYQRLLNELDEALNFLIDCNISTHSKERDSTLISKQILSDCQTVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTILRKIHALCEEHFSPASLDMKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDEDEEIEEKEKTETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPSEYRSYVQSKGRARAPISNYIMLADSDKIKAFEEDLKTYKAIEKILRNKCSKSIDCGNTESEPIVDDDEIFPPYVLRQDDGSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTREFPDGLYRSTLYLPINSPLRAPIVGPPMNCGRLADRAVALICCKKLHEIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLNLEQLELITRLHQYIFSHILRLEKPALEFKPTVADCAYCVLPLNVVNDSGTLDIDFKFVEDIEKSEARTGIPNTQYSAESPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPVPASLWRKAVCLPSILYRLHCLLTAEELRAQTAIDAGVGVKSLPDDFRYPNLDFGWKRSIDSKTFISNQSSSSVESESDCRLNKTTAPDSAASSAANSVIYMQINDQMSVNCTPPCQKSLSHLQTVCFSDDYKAINGISCNGLTNGDWEAESAACFQKDERITCKQEIPEKSTSFHVQNLPKENQPILKECTLSNSDGNVSKPTSDECPSTCTSDMHYDSGLSNRHSSKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGSPSRMVVSIFDPPVNWLPPGYIVNQDKNSDKWESNETSGEDVMVNGKIDEDFDDEEDEDLMWRNPKEETDFDDDFLEYDQEHIKFIDSMLMGSGAFVKKIPLSSFAPPDQNYEWRAPKKPPLESSQFPCDFDDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGADAGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPEVRKLVTNTNVISASSSYQNSTRDNCTLTARTNTDLSSCKGIDYGYLKIPPRCMFEHPDAEKTLDHLISGFENFEKKINYPFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWHVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQSQVPNS

Target ORF information:

RefSeq Version	XM_012968075.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis dicer 1, ribonuclease type III (dicer1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968075.2

ORF Insert Sequence:

ATGGCAGGCC TTCAGCTCAT GACTCCGGCC TCCTCTCCAA TGGGTCCCTT CTTTGGGCTA 
CCCTGGCAAC AGGAGGCTAT TCATGACAAC ATTTATACCC CACGGAAATA TCAGGTGGAA 
CTACTCGAAG CAGCATTGGA TCATAATACT ATAGTATGTT TGAATTCTGG CTCAGGGAAG 
ACATTTATTG CAGTACTACT CAGTAAAGAG CTGTCATACC AGATCCGTGG GGACTTCAGC 
AAAAATACTA AGAGGACTGT GTTTTTGGTC AATTCCGAGA AACAGGTTTC TCAACAAGTG 
TCTGCTGTCA GGACCCATAC AGACCTCAAG GTGGGAGAAT ATTCAGATCA AGAAAAAACA 
CAATGCTGGG CAAAAGAAAG ATGGTACCTA GAATTTGAAA CTCATCAGGT CTTGGTCATG 
ACCTGCCACA TCTTCCTGAA CGTTCTGAAA AGTGGGAACG TGTCATTGTC AAACATTAAT 
CTGTTAGTGT TTGATGAATG TCATCTTGCA ATTCAGGATC ACCCATATCG AGAAATCATG 
AAGATATGTG AGAGTTGCCA GCCATGCCCT CGAATCCTGG GTCTAACTGC TTCAATTTTA 
AATGGAAAAT GTGACCCTCG TGACCTAGAG GAAAAGATCC AGAAACTGGA GGAAATATTA 
AGGAGTAATG CAGAAACTGC AACTGATTTG GTTGTTTTAG ACAGGTATGC TTCCCAACCA 
TGTGAAATTG TATTGGACTG TGGGCCATAT ATTGACAAAA GTGGACTTTA TCAAAGACTT 
CTAAATGAAT TGGATGAAGC CCTTAACTTT CTCATTGACT GTAATATTTC TACACATTCT 
AAAGAGAGAG ATTCCACATT AATTTCAAAA CAGATTCTAT CAGACTGTCA AACTGTTCTT 
TTGGTCTTGG GACCATGGTG TGCTGATAAA GTTGCGGGGA TGATGGTGAG AGAGCTGCAG 
AAGTATATCA AACATGAGCA GGAGGAACTG CACAGAAAAT TCCTTTTGTT TACAGACACT 
ATCTTAAGGA AGATCCATGC TCTTTGTGAG GAACACTTCT CGCCAGCCTC TCTTGATATG 
AAGTTTGTCA CACCTAAAGT TATAAAACTG CTGGAAATTT TACGCAAATA CAAACCCTAC 
GAACGCCAGC AATTTGAAAG TGTTGAATGG TATAACAATA GAAACCAGGA TAATTATGTG 
TCTTGGAGTG ATTCAGAGGA TGATGACGAT GAAGATGAAG AAATTGAGGA GAAAGAGAAA 
ACTGAAACAA GCTTTCCATC CCCATTCACA AACATCCTGT GTGGCATCAT CTTCGTGGAA 
CGAAGATACA CAGCAGTAGT ATTAAACAGG TTGATTAAAG AAGCCGGGAA GCAAGATCCA 
GAGCTGGCCT ATATCAGTAG TAACTTTATT ACTGGGCACG GCATAGGAAA GAACCAGCCA 
CGCAATAAGC AGATGGAAGT TGAGTTTAGA AAGCAAGAAG AGGTGCTTCG TAAATTTCGT 
GCACACGAAA CCAACTTATT GATAGCTACT AGCATTGTTG AGGAAGGAGT GGACATACCA 
AAATGCAACT TGGTAGTTCG ATTTGATTTA CCTTCAGAGT ACAGATCCTA TGTACAGTCC 
AAAGGCAGAG CAAGAGCACC AATCTCAAAT TACATCATGC TAGCCGATAG TGATAAAATT 
AAGGCATTTG AAGAGGACCT TAAAACATAC AAAGCAATTG AAAAGATTCT GCGGAACAAA 
TGCTCAAAGT CCATTGATTG TGGAAATACA GAATCTGAGC CCATTGTGGA CGATGATGAA 
ATATTTCCAC CATATGTGTT GAGACAGGAT GATGGCAGCC CACGAGTTAC TATCAACACC 
GCTATTGGAC ACATTAACAG GTACTGTGCT AGGCTACCTA GTGACCCATT TACTCATCTT 
GCTCCTAAGT GCAAAACACG AGAGTTCCCT GATGGACTTT ATCGCTCAAC ACTCTACCTG 
CCAATTAATT CTCCACTTAG AGCCCCCATT GTTGGCCCTC CGATGAATTG TGGAAGGCTA 
GCTGATAGAG CTGTAGCTCT TATATGCTGT AAAAAACTAC ATGAAATTGG TGAACTGGAT 
GATCATTTAA TGCCAGTTGG CAAGGAAACT GTAAAATATG AGGAGGAGCT TGATTTGCAT 
GATGAGGAAG AAACCAGTGT TCCAGGCAGA CCAGGATCCA CAAAAAGAAG GCAGTGTTAT 
CCAAAAGCTA TTCCTGAATG TTTACGGAAC AGCTACCCCA AGCCTGGTCA GCCTTGTTAC 
TTATATGTAA TAGGAATGGT ATTAACCACT CCTCTACCAG ATGAACTTAA TTTTAGGCGA 
CGGAAGCTGT ATCCCCCTGA AGACACAACA AGATGCTTTG GAATACTAAC TGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTCTAT ACTCGTTCGG GAGAGGTGAC CATATCAATT 
GAACTAAAGA AGTCTGGTTT TACATTAAAC TTAGAACAAC TTGAGCTCAT TACTAGACTC 
CACCAGTACA TTTTTTCACA CATTCTTCGT CTTGAGAAAC CTGCACTAGA ATTTAAACCC 
ACAGTAGCCG ATTGTGCCTA CTGTGTTCTA CCTCTTAACG TTGTAAATGA TTCTGGCACT 
TTGGACATTG ACTTCAAATT TGTAGAAGAT ATTGAGAAAT CAGAAGCACG TACTGGTATA 
CCTAATACAC AGTATTCAGC TGAAAGTCCT TTTATTTTCA AATTAGAAGA CTACCAGGAT 
GCTGTTATCA TTCCAAGATA TCGAAATTTT GATCAGCCGC ACAGATTTTA TGTAGCTGAT 
GTATACACTG ATCTTACACC GCTCAGCAAG TTCCCTTCCC CTGAATATGA AACCTTTGCT 
GAATATTACA AAACAAAGTA TAACCTCGAT CTCACAAACC TCAATCAGCC ACTTTTGGAT 
GTGGACCACA CATCATCAAG ACTTAATTTG CTGACTCCTC GCCATCTGAA TCAGAAAGGT 
AAAGCTCTGC CATTAAGCAG TGCTGAAAAG AGAAAAGCCA AATGGGAGAG TTTACAAAAC 
AAACAGATCC TGGTTCCAGA GCTTTGTGCT ATACATCCAG TCCCAGCATC CTTATGGAGA 
AAAGCTGTTT GTTTGCCAAG CATACTTTAT CGATTACATT GCCTCCTTAC AGCAGAAGAG 
CTGAGAGCGC AAACAGCTAT TGATGCTGGG GTAGGAGTCA AATCGCTTCC TGATGATTTC 
CGATACCCAA ACTTGGATTT TGGATGGAAA CGATCTATAG ACAGTAAAAC ATTCATCTCT 
AATCAAAGTT CCTCATCAGT CGAGAGTGAA AGTGACTGCA GACTCAATAA AACCACGGCC 
CCTGACAGTG CTGCAAGCTC AGCTGCTAAT TCTGTAATCT ACATGCAAAT CAATGACCAA 
ATGTCTGTGA ACTGCACACC ACCATGTCAA AAATCTCTGA GTCACCTCCA AACAGTTTGC 
TTCTCAGATG ATTACAAAGC AATTAACGGT ATTTCTTGCA ATGGTCTTAC CAATGGTGAT 
TGGGAAGCAG AAAGCGCTGC ATGTTTCCAA AAGGACGAAC GGATAACCTG CAAACAGGAA 
ATACCTGAAA AATCTACCTC GTTTCACGTT CAGAATTTAC CTAAGGAGAA CCAGCCCATA 
CTTAAAGAAT GTACTCTGAG CAACTCTGAT GGAAATGTCA GCAAACCTAC CTCAGATGAG 
TGCCCTAGCA CCTGTACTTC AGACATGCAT TATGATAGTG GGCTTTCCAA TAGGCACTCT 
TCAAAAACTC TTGGCCCAAA CCCTGGTCTT ATCCTTCAGG CCTTGACTCT GTCCAATGCA 
AGTGATGGTT TCAATCTGGA GCGTCTTGAA ATGCTTGGGG ACTCTTTCTT AAAACATGCT 
ATCACCACAT ATCTGTTCTG CACATACCCA GATGCTCACG AGGGCCGTCT ATCCTATATG 
CGCAGCAAGA AGGTCAGTAA CTGTAACCTT TATCGGCTTG GAAAAAAGAA AGGCTCACCC 
AGCCGGATGG TGGTGTCCAT TTTCGATCCA CCTGTCAACT GGCTTCCCCC TGGTTATATT 
GTTAACCAGG ACAAAAACTC TGATAAGTGG GAAAGTAATG AAACGTCTGG TGAGGATGTG 
ATGGTTAATG GCAAAATTGA TGAAGACTTT GATGATGAAG AAGATGAAGA TCTTATGTGG 
AGGAATCCCA AAGAGGAAAC TGATTTTGAT GATGACTTTT TGGAATATGA TCAAGAGCAT 
ATAAAATTCA TTGACAGCAT GTTAATGGGA TCTGGGGCAT TTGTGAAGAA AATTCCTCTT 
TCTTCATTTG CACCCCCTGA TCAGAACTAT GAATGGAGGG CGCCAAAGAA ACCTCCACTG 
GAAAGTTCTC AATTTCCCTG CGATTTTGAT GATTTTGATT ACAGTTCTTG GGATGCCATG 
TGTTATTTGG ATCCCAGCAA GGCTGTAGAG GAGGATGACT TTGTAGTAGG CTTTTGGAAT 
CCATCAGAAG AGAACTGTGG AGCTGATGCT GGAAAGCAGT CTATATCTTA TGACCTACAC 
ACAGAGCAGT GTATTGCAGA CAAAAGTATA GCTGACTGTG TAGAGGCTTT ATTAGGCTGC 
TATCTGACCA GTTGTGGTGA GAGAGCTGCA CAACTGTTTC TTTGTTCTTT GGGCTTAAAA 
GTGCTCCCAG AAGTAAGAAA GCTTGTTACA AACACAAATG TCATTAGTGC ATCATCATCT 
TACCAGAACA GTACCAGAGA CAACTGCACT CTTACTGCCA GAACCAACAC TGATCTGTCC 
TCCTGCAAAG GCATAGACTA CGGTTATTTA AAGATTCCCC CAAGGTGTAT GTTTGAGCAC 
CCAGATGCTG AGAAAACCCT TGACCATCTA ATATCTGGTT TTGAAAACTT TGAGAAGAAA 
ATAAACTATC CATTTAAAAA CAAGGCTTAC CTTCTACAGG CTTTTACACA TGCCTCTTAC 
CACTACAATA CTATAACTGA CTGCTACCAG CGTTTAGAAT TTCTTGGAGA TGCAATTTTG 
GACTACCTCA TTACTAAGCA CCTTTATGAA GATCCACGCC AGCACTCGCC AGGAGTCCTC 
ACTGACCTGC GCTCTGCTCT TGTGAATAAC ACCATATTTG CATCACTAGC TGTGAAATAT 
GACTACCATA AATACTTCAA GGCTATCTCT CCTGAACTGT TCCATGTAAT TGATGATTTT 
GTGCAATTTC AACTTGAAAA AAATGAAATG CAAGGCATGG ATTCTGAGTT ACGTAGGTCT 
GAAGAAGACG AAGAGAAAGA AGAGGACATT GAAGTCCCAA AAGCTATGGG AGACATATTT 
GAGTCACTTG CAGGAGCTAT TTACATGGAC AGCGGCATGT CTTTGGAAAC TGTATGGCAT 
GTGTATTATC CTATGATGCA GCCATTAATA GAAAAATTCT CTGCTAATGT TCCTCGTTCC 
CCAGTGAGAG AATTACTGGA AATGGAGCCT GAAACTGCTA AATTCAGTCC AGCCGAAAGA 
ACATACGATG GAAAAGTCAG AGTGACTGTG GAAGTTGTTG GAAAAGGAAA ATTTAAAGGA 
GTCGGAAGAA GTTACAGAAT TGCCAAATCT GCAGCAGCCA GGAGAGCACT AAGAAGCCTC 
AAAGCAAATC AATCTCAGGT CCCTAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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dicer1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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