DICER1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	DICER1
Entrez Gene ID	480426
Full Name	dicer 1, ribonuclease III
General protein information	Preferred Names dicer 1, ribonuclease III Names endoribonuclease Dicer Dicer1, Dcr-1 homolog
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_863433.4	XP_868526.4	endoribonuclease Dicer
XM_014116140.2	XP_013971615.1	endoribonuclease Dicer
XM_022422353.1	XP_022278061.1	endoribonuclease Dicer
XM_863433.5	XP_868526.4	endoribonuclease Dicer
XM_014116140.1	XP_013971615.1	endoribonuclease Dicer

Pathways from BioSystems

Homologies

Homo sapiens (human)	DICER1	NP_085124.2
Rattus norvegicus (Norway rat)	Dicer1	XP_006240573.1
Danio rerio (zebrafish)	dicer1	NP_001154925.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002836	XP_003436256.1
Magnaporthe oryzae (rice blast fungus)	MGG_01541	XP_003714515.1
Neurospora crassa	NCU08270	XP_961898.1
	DICER1	XP_001100868.1
Gallus gallus (chicken)	DICER1	NP_001035555.1
Drosophila melanogaster (fruit fly)	Dcr-1	NP_524453.1
Caenorhabditis elegans (roundworm)	dcr-1	NP_498761.2
Xenopus tropicalis (tropical clawed frog)	dicer1	NP_001123390.2
Canis lupus familiaris (dog)	DICER1	XP_868526.3
Bos taurus (cattle)	DICER1	NP_976235.1
Schizosaccharomyces pombe (fission yeast)	dcr1	NP_588215.2
Pan troglodytes (chimpanzee)	DICER1	XP_001154369.1
Mus musculus (house mouse)	Dicer1	NP_683750.2

Gene Ontology

The following DICER1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DICER1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe38040	XM_014116140.2 Latest version!	Canis lupus familiaris dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe38040	XM_022422353.1 Latest version!	Canis lupus familiaris dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe38040	XM_863433.5 Latest version!	Canis lupus familiaris dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
View Old Accession Versions >>
OCe38040	XM_863433.4	Canis lupus familiaris dicer 1, ribonuclease type III (DICER1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe38040	XM_014116140.1	Canis lupus familiaris dicer 1, ribonuclease type III (DICER1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DICER1 ( XM_863433.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_868526.4 Canis lupus familiaris dicer 1, ribonuclease type III (DICER1), transcript variant X2, mRNA.

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CloneID	OCe38040
Clone ID Related Accession (Same CDS sequence)	XM_863433.4 , XM_014116140.2 , XM_022422353.1 , XM_863433.5 , XM_014116140.1
Accession Version	XM_863433.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	endoribonuclease Dicer
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_863433.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCC 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAAC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCTCTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCGTGTCCT 
CGTATTTTGG GACTCACTGC TTCCATTTTA AATGGGAAAT GCGATCCCGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTG AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTATTGG ACAGATACAC TTCTCAACCC TGTGAGATTG TGGTGGACTG TGGGCCATTC 
ACGGACAGAA GTGGCCTCTA TGGCAGACTG CTGGTGGAGC TAGAGGAAGC ACTTAACTTT 
ATCAACGACT GTAACATATC TGTGCATTCG AAAGAAAGAG ATTCTACTCT AATTTCTAAA 
CAGATACTGT CAGACTGTCG TGCGGTATTG GTAGTCCTGG GCCCCTGGTG TGCAGATAAA 
GTAGCTGGGA TGATGGTAAG AGAACTGCAA AAGTATATCA AACATGAACA GGAAGAGCTG 
CACAGGAAAT TTCTATTGTT TACAGACACC TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CTCCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATAAAACTG 
CTTGAAATCC TACGCAAATA TAAACCGTAC GAGCGACAGC AGTTTGAAAG TGTCGAGTGG 
TATAATAATA GGAATCAGGA TAATTATGTG TCTTGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACCAACT TTCCTTCTCC ATTTACCAAT 
ATTTTATGTG GAATCATTTT TGTGGAAAGA AGATACACGG CTGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTTCTCAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGATT TGATTTGCCC 
ACAGAGTATC GATCCTATGT TCAATCTAAG GGAAGAGCAA GGGCACCGAT CTCTAATTAT 
ATAATGTTAG CAGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTAAG AAACAAGTGT TCCAAGTCAG TTGACACTGG TGAGACTGAC 
ATTGAACCTG TTGTGGATGA TGACGACGTG TTCCCACCAT ACGTGTTGAG GCCTGATGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGTA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTCCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCC GAAAGAGTCG TAGCTCTCAT TTGCTGTGAA 
AAACTGCACA AAATTGGTGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACCGTT 
AAATATGAAG AAGAGCTTGA TTTACATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACAA AACGAAGACA GTGCTACCCA AAAGCAATTC CAGAATGTTT AAGGGATAGC 
TATCCCAAAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTCT GACGACGCCC 
TTACCTGATG AACTCAACTT CAGAAGGCGC AAGCTCTATC CCCCTGAGGA TACCACACGA 
TGCTTTGGAA TACTAACAGC CAAACCTATA CCTCAGATCC CGCACTTTCC TGTGTACACG 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAC ATTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCCACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TGAATGACTC CAGCACTTTG GACATTGACT TTAAATTCAT GGAAGATATT 
GAGAAATCTG AAGCTCGCAT AGGCATTCCC AGTACCAAGT ATTCAAAAGA AACACCTTTT 
GTTTTTAAAT TAGAAGATTA CCAGGATGCA GTTATCATTC CAAGATATCG TAATTTTGAT 
CAGCCTCATC GATTCTATGT AGCTGATGTA TACACTGATC TTACCCCACT GAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAATACAA CCTTGACCTG 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGTC ATTTGAATCA GAAGGGGAAA GCGCTTCCTC TTAGCAGTGC TGAAAAGAGA 
AAAGCCAAGT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAGCT CTGTGCTATA 
CACCCAATTC CAGCGTCACT GTGGCGAAAA GCAGTCTGTC TCCCTAGTAT ACTCTATCGC 
CTTCACTGCC TTTTGACCGC AGAGGAGCTA AGAGCCCAGA CGGCCAGCGA TGCTGGCGTG 
GGAGTCCGAT CACTTCCCGT GGATTTTAGA TACCCTAACC TAGACTTTGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAGTT GCTAACTCCT CTTCAGCTGA AAATGAAAAT 
TACTGTAAGC ACAGCACAAT TGTCGTCCCT GAAAATGCTG CACGTCAAGG TGCTAATAGA 
ACCTCCTCTC TAGAAAATCA TGACCAAATG TCTGTGAACT GCAGAACGTT ATTCAGTGAG 
TCACCTGGTA AGCTCCAGAT TGAAGTTGTA ACAGATCTTA CAGCAATTAA TGGTCTTTCT 
TATAATAAAA ATCTTGCCAA TGGCAGTTAT GATTTAGCTA ACAGAGACTT TTGCCAAGGA 
AATCAGCTGA ATTACTACAA GCAGGAAATA CCTGTACAAC CAACTACCTC ATATCCCATT 
CAGAATTTAT ACAATTACGA GAACCAGCCC AAGCCCAGCG ATGAATGTAC TCTACTGAGT 
AATAAATACC TTGATGGAAA TGCTAACAAA TCTACCTCAG ATGGAAGTCC CACGACGGCC 
GCAATGCCTG GTACTACCGA AGCTGTTCGA GCGCTCAAGG ACAAGATGGG TTCTGAGCAG 
AGCCCTTGTC CTGGGTACTC CTCGAGGACT CTTGGCCCAA ATCCTGGACT TATTCTGCAG 
GCTTTGACCC TTTCAAATGC TAGTGATGGA TTTAACCTGG AGCGGCTTGA AATGCTTGGT 
GACTCCTTTT TAAAGCATGC CATCACCACA TATCTATTTT GCACTTATCC TGATGCTCAT 
GAGGGCCGCC TTTCATATAT GAGAAGCAAA AAGGTCAGCA ACTGTAATCT GTATCGGCTT 
GGAAAAAAGA AGGGACTGCC CAGCCGCATG GTGGTATCAA TATTTGATCC CCCTGTGAAT 
TGGCTTCCTC CTGGTTATGT AGTAAACCAA GACAAAAGTA ACGCAGATAA ATGGGAAAAA 
GATGAAATGA CAAAAGACTG CATGTTGGCT AATGGCAAGC TGGACGAGGA CTTTGAGGAG 
GACGACGAGG AGGAGGAGGA TCTAATGTGG AGGGCTCCGA AGGAGGACGC CGATTATGAA 
GATGATTTCC TGGAGTATGA TCAGGAACAT ATCAAATTTA TAGATAACAT GTTAATGGGA 
TCAGGAGCTT TTGTAAAGAA AATTTCTCTC TCTCCTTTTT CAACCACTGA TTCTGCATAC 
GAATGGAAAA TGCCCAAAAA ATCCTCCTTA GGTAGTATGC CATTTTCATC AGATTTTGAG 
GATTTTGACT ATAGCTCTTG GGATGCCATG TGCTATCTGG ATCCTAGCAA AGCTGTGGAA 
GAGGATGACT TTGTGGTGGG GTTCTGGAAT CCATCAGAAG AAAACTGTGG TGTTGACACG 
GGGAAACAGT CCATTTCTTA CGACTTGCAC ACTGAGCAGT GTATTGCGGA CAAAAGCATA 
GCCGACTGCG TGGAAGCCCT GCTGGGCTGC TATTTAACCA GCTGCGGCGA GAGGGCTGCT 
CAGCTCTTCC TCTGCTCACT GGGGCTCAAG GTGCTCCCGG TCATGAAAAG GACCGATCGG 
GAAAAGACCA TGTGCCCTCC TAGGGAGAAT TTCAGCAGCC AACAAAAGAA CCTTTCAGGG 
GGCCGCGCTG CTGCCTCTGT GGCCAGTTTA CGTCCCTCTG TGTTGAAAGA CTTGGAGTAT 
GGCTGTTTGA AGATTCCACC GAGGTGTATG TTTGATCATC CGGACGCAGA TAAAACACTG 
AATCACCTTA TATCGGGTTT TGAGAATTTT GAAAAGAAAA TCAACTACAG ATTCAAGAAT 
AAGGCTTATC TTCTACAGGC TTTTACCCAT GCCTCCTACC ACTACAATAC TATTACTGAT 
TGTTACCAGC GCCTGGAGTT CCTGGGAGAT GCGATCTTGG ACTACCTCAT AACCAAGCAC 
CTTTACGAAG ACCCGCGGCA GCACTCCCCG GGCGTCCTGA CCGACCTGCG GTCCGCACTG 
GTCAACAACA CCATCTTCGC GTCGCTGGCG GTCAAGTACG ACTACCACAA GTACTTCAAA 
GCTGTCTCCC CTGAGCTCTT CCATGTCATT GATGACTTCG TGCAGTTCCA GCTGGAGAAG 
AATGAGATGC AGGGCATGGA CTCCGAGCTT AGGAGGTCTG AGGAGGATGA AGAGAAAGAA 
GAGGACATTG AAGTTCCAAA GGCCATGGGG GACATTTTTG AGTCGCTTGC TGGTGCCATT 
TACATGGATA GTGGGATGTC ACTGGAGATG GTTTGGCAGG TGTACTATCC TATGATGCGG 
CCGCTGATAG AAAAATTTTC TGCAAACGTG CCCCGTTCCC CTGTGCGAGA ATTGCTTGAA 
ATGGAACCAG AAACTGCCAA ATTTAGCCCG GCTGAGAGGA CGTATGACGG CAAGGTCAGA 
GTCACGGTGG AAGTGGTAGG GAAGGGGAAA TTTAAAGGCG TCGGCCGGAG CTACAGGATC 
GCCAAGTCTG CAGCAGCAAG AAGAGCCCTA CGCAGCCTCA AAGCCAATCA ACCTCAGGTT 
CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_868526.4
CDS	418..6189
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYGRLLVELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIEPVVDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAARQGANRTSSLENHDQMSVNCRTLFSESPGKLQIEVVTDLTAINGLSYNKNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANKSTSDGSPTTAAMPGTTEAVRALKDKMGSEQSPCPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNADKWEKDEMTKDCMLANGKLDEDFEEDDEEEEDLMWRAPKEDADYEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVMKRTDREKTMCPPRENFSSQQKNLSGGRAAASVASLRPSVLKDLEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEMVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_863433.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dicer 1, ribonuclease type III (DICER1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_863433.4

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCC 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAAC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCTCTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCGTGTCCT 
CGTATTTTGG GACTCACTGC TTCCATTTTA AATGGGAAAT GCGATCCCGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTG AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTATTGG ACAGATACAC TTCTCAACCC TGTGAGATTG TGGTGGACTG TGGGCCATTC 
ACGGACAGAA GTGGCCTCTA TGGCAGACTG CTGGTGGAGC TAGAGGAAGC ACTTAACTTT 
ATCAACGACT GTAACATATC TGTGCATTCG AAAGAAAGAG ATTCTACTCT AATTTCTAAA 
CAGATACTGT CAGACTGTCG TGCGGTATTG GTAGTCCTGG GCCCCTGGTG TGCAGATAAA 
GTAGCTGGGA TGATGGTAAG AGAACTGCAA AAGTATATCA AACATGAACA GGAAGAGCTG 
CACAGGAAAT TTCTATTGTT TACAGACACC TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CTCCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATAAAACTG 
CTTGAAATCC TACGCAAATA TAAACCGTAC GAGCGACAGC AGTTTGAAAG TGTCGAGTGG 
TATAATAATA GGAATCAGGA TAATTATGTG TCTTGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACCAACT TTCCTTCTCC ATTTACCAAT 
ATTTTATGTG GAATCATTTT TGTGGAAAGA AGATACACGG CTGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTTCTCAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGATT TGATTTGCCC 
ACAGAGTATC GATCCTATGT TCAATCTAAG GGAAGAGCAA GGGCACCGAT CTCTAATTAT 
ATAATGTTAG CAGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTAAG AAACAAGTGT TCCAAGTCAG TTGACACTGG TGAGACTGAC 
ATTGAACCTG TTGTGGATGA TGACGACGTG TTCCCACCAT ACGTGTTGAG GCCTGATGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGTA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTCCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCC GAAAGAGTCG TAGCTCTCAT TTGCTGTGAA 
AAACTGCACA AAATTGGTGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACCGTT 
AAATATGAAG AAGAGCTTGA TTTACATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACAA AACGAAGACA GTGCTACCCA AAAGCAATTC CAGAATGTTT AAGGGATAGC 
TATCCCAAAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTCT GACGACGCCC 
TTACCTGATG AACTCAACTT CAGAAGGCGC AAGCTCTATC CCCCTGAGGA TACCACACGA 
TGCTTTGGAA TACTAACAGC CAAACCTATA CCTCAGATCC CGCACTTTCC TGTGTACACG 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAC ATTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCCACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TGAATGACTC CAGCACTTTG GACATTGACT TTAAATTCAT GGAAGATATT 
GAGAAATCTG AAGCTCGCAT AGGCATTCCC AGTACCAAGT ATTCAAAAGA AACACCTTTT 
GTTTTTAAAT TAGAAGATTA CCAGGATGCA GTTATCATTC CAAGATATCG TAATTTTGAT 
CAGCCTCATC GATTCTATGT AGCTGATGTA TACACTGATC TTACCCCACT GAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAATACAA CCTTGACCTG 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGTC ATTTGAATCA GAAGGGGAAA GCGCTTCCTC TTAGCAGTGC TGAAAAGAGA 
AAAGCCAAGT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAGCT CTGTGCTATA 
CACCCAATTC CAGCGTCACT GTGGCGAAAA GCAGTCTGTC TCCCTAGTAT ACTCTATCGC 
CTTCACTGCC TTTTGACCGC AGAGGAGCTA AGAGCCCAGA CGGCCAGCGA TGCTGGCGTG 
GGAGTCCGAT CACTTCCCGT GGATTTTAGA TACCCTAACC TAGACTTTGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAGTT GCTAACTCCT CTTCAGCTGA AAATGAAAAT 
TACTGTAAGC ACAGCACAAT TGTCGTCCCT GAAAATGCTG CACGTCAAGG TGCTAATAGA 
ACCTCCTCTC TAGAAAATCA TGACCAAATG TCTGTGAACT GCAGAACGTT ATTCAGTGAG 
TCACCTGGTA AGCTCCAGAT TGAAGTTGTA ACAGATCTTA CAGCAATTAA TGGTCTTTCT 
TATAATAAAA ATCTTGCCAA TGGCAGTTAT GATTTAGCTA ACAGAGACTT TTGCCAAGGA 
AATCAGCTGA ATTACTACAA GCAGGAAATA CCTGTACAAC CAACTACCTC ATATCCCATT 
CAGAATTTAT ACAATTACGA GAACCAGCCC AAGCCCAGCG ATGAATGTAC TCTACTGAGT 
AATAAATACC TTGATGGAAA TGCTAACAAA TCTACCTCAG ATGGAAGTCC CACGACGGCC 
GCAATGCCTG GTACTACCGA AGCTGTTCGA GCGCTCAAGG ACAAGATGGG TTCTGAGCAG 
AGCCCTTGTC CTGGGTACTC CTCGAGGACT CTTGGCCCAA ATCCTGGACT TATTCTGCAG 
GCTTTGACCC TTTCAAATGC TAGTGATGGA TTTAACCTGG AGCGGCTTGA AATGCTTGGT 
GACTCCTTTT TAAAGCATGC CATCACCACA TATCTATTTT GCACTTATCC TGATGCTCAT 
GAGGGCCGCC TTTCATATAT GAGAAGCAAA AAGGTCAGCA ACTGTAATCT GTATCGGCTT 
GGAAAAAAGA AGGGACTGCC CAGCCGCATG GTGGTATCAA TATTTGATCC CCCTGTGAAT 
TGGCTTCCTC CTGGTTATGT AGTAAACCAA GACAAAAGTA ACGCAGATAA ATGGGAAAAA 
GATGAAATGA CAAAAGACTG CATGTTGGCT AATGGCAAGC TGGACGAGGA CTTTGAGGAG 
GACGACGAGG AGGAGGAGGA TCTAATGTGG AGGGCTCCGA AGGAGGACGC CGATTATGAA 
GATGATTTCC TGGAGTATGA TCAGGAACAT ATCAAATTTA TAGATAACAT GTTAATGGGA 
TCAGGAGCTT TTGTAAAGAA AATTTCTCTC TCTCCTTTTT CAACCACTGA TTCTGCATAC 
GAATGGAAAA TGCCCAAAAA ATCCTCCTTA GGTAGTATGC CATTTTCATC AGATTTTGAG 
GATTTTGACT ATAGCTCTTG GGATGCCATG TGCTATCTGG ATCCTAGCAA AGCTGTGGAA 
GAGGATGACT TTGTGGTGGG GTTCTGGAAT CCATCAGAAG AAAACTGTGG TGTTGACACG 
GGGAAACAGT CCATTTCTTA CGACTTGCAC ACTGAGCAGT GTATTGCGGA CAAAAGCATA 
GCCGACTGCG TGGAAGCCCT GCTGGGCTGC TATTTAACCA GCTGCGGCGA GAGGGCTGCT 
CAGCTCTTCC TCTGCTCACT GGGGCTCAAG GTGCTCCCGG TCATGAAAAG GACCGATCGG 
GAAAAGACCA TGTGCCCTCC TAGGGAGAAT TTCAGCAGCC AACAAAAGAA CCTTTCAGGG 
GGCCGCGCTG CTGCCTCTGT GGCCAGTTTA CGTCCCTCTG TGTTGAAAGA CTTGGAGTAT 
GGCTGTTTGA AGATTCCACC GAGGTGTATG TTTGATCATC CGGACGCAGA TAAAACACTG 
AATCACCTTA TATCGGGTTT TGAGAATTTT GAAAAGAAAA TCAACTACAG ATTCAAGAAT 
AAGGCTTATC TTCTACAGGC TTTTACCCAT GCCTCCTACC ACTACAATAC TATTACTGAT 
TGTTACCAGC GCCTGGAGTT CCTGGGAGAT GCGATCTTGG ACTACCTCAT AACCAAGCAC 
CTTTACGAAG ACCCGCGGCA GCACTCCCCG GGCGTCCTGA CCGACCTGCG GTCCGCACTG 
GTCAACAACA CCATCTTCGC GTCGCTGGCG GTCAAGTACG ACTACCACAA GTACTTCAAA 
GCTGTCTCCC CTGAGCTCTT CCATGTCATT GATGACTTCG TGCAGTTCCA GCTGGAGAAG 
AATGAGATGC AGGGCATGGA CTCCGAGCTT AGGAGGTCTG AGGAGGATGA AGAGAAAGAA 
GAGGACATTG AAGTTCCAAA GGCCATGGGG GACATTTTTG AGTCGCTTGC TGGTGCCATT 
TACATGGATA GTGGGATGTC ACTGGAGATG GTTTGGCAGG TGTACTATCC TATGATGCGG 
CCGCTGATAG AAAAATTTTC TGCAAACGTG CCCCGTTCCC CTGTGCGAGA ATTGCTTGAA 
ATGGAACCAG AAACTGCCAA ATTTAGCCCG GCTGAGAGGA CGTATGACGG CAAGGTCAGA 
GTCACGGTGG AAGTGGTAGG GAAGGGGAAA TTTAAAGGCG TCGGCCGGAG CTACAGGATC 
GCCAAGTCTG CAGCAGCAAG AAGAGCCCTA CGCAGCCTCA AAGCCAATCA ACCTCAGGTT 
CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_014116140.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013971615.1 Canis lupus familiaris dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.

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CloneID	OCe38040
Clone ID Related Accession (Same CDS sequence)	XM_863433.4 , XM_014116140.2 , XM_022422353.1 , XM_863433.5 , XM_014116140.1
Accession Version	XM_014116140.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	endoribonuclease Dicer
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116140.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCC 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAAC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCTCTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCGTGTCCT 
CGTATTTTGG GACTCACTGC TTCCATTTTA AATGGGAAAT GCGATCCCGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTG AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTATTGG ACAGATACAC TTCTCAACCC TGTGAGATTG TGGTGGACTG TGGGCCATTC 
ACGGACAGAA GTGGCCTCTA TGGCAGACTG CTGGTGGAGC TAGAGGAAGC ACTTAACTTT 
ATCAACGACT GTAACATATC TGTGCATTCG AAAGAAAGAG ATTCTACTCT AATTTCTAAA 
CAGATACTGT CAGACTGTCG TGCGGTATTG GTAGTCCTGG GCCCCTGGTG TGCAGATAAA 
GTAGCTGGGA TGATGGTAAG AGAACTGCAA AAGTATATCA AACATGAACA GGAAGAGCTG 
CACAGGAAAT TTCTATTGTT TACAGACACC TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CTCCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATAAAACTG 
CTTGAAATCC TACGCAAATA TAAACCGTAC GAGCGACAGC AGTTTGAAAG TGTCGAGTGG 
TATAATAATA GGAATCAGGA TAATTATGTG TCTTGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACCAACT TTCCTTCTCC ATTTACCAAT 
ATTTTATGTG GAATCATTTT TGTGGAAAGA AGATACACGG CTGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTTCTCAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGATT TGATTTGCCC 
ACAGAGTATC GATCCTATGT TCAATCTAAG GGAAGAGCAA GGGCACCGAT CTCTAATTAT 
ATAATGTTAG CAGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTAAG AAACAAGTGT TCCAAGTCAG TTGACACTGG TGAGACTGAC 
ATTGAACCTG TTGTGGATGA TGACGACGTG TTCCCACCAT ACGTGTTGAG GCCTGATGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGTA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTCCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCC GAAAGAGTCG TAGCTCTCAT TTGCTGTGAA 
AAACTGCACA AAATTGGTGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACCGTT 
AAATATGAAG AAGAGCTTGA TTTACATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACAA AACGAAGACA GTGCTACCCA AAAGCAATTC CAGAATGTTT AAGGGATAGC 
TATCCCAAAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTCT GACGACGCCC 
TTACCTGATG AACTCAACTT CAGAAGGCGC AAGCTCTATC CCCCTGAGGA TACCACACGA 
TGCTTTGGAA TACTAACAGC CAAACCTATA CCTCAGATCC CGCACTTTCC TGTGTACACG 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAC ATTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCCACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TGAATGACTC CAGCACTTTG GACATTGACT TTAAATTCAT GGAAGATATT 
GAGAAATCTG AAGCTCGCAT AGGCATTCCC AGTACCAAGT ATTCAAAAGA AACACCTTTT 
GTTTTTAAAT TAGAAGATTA CCAGGATGCA GTTATCATTC CAAGATATCG TAATTTTGAT 
CAGCCTCATC GATTCTATGT AGCTGATGTA TACACTGATC TTACCCCACT GAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAATACAA CCTTGACCTG 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGTC ATTTGAATCA GAAGGGGAAA GCGCTTCCTC TTAGCAGTGC TGAAAAGAGA 
AAAGCCAAGT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAGCT CTGTGCTATA 
CACCCAATTC CAGCGTCACT GTGGCGAAAA GCAGTCTGTC TCCCTAGTAT ACTCTATCGC 
CTTCACTGCC TTTTGACCGC AGAGGAGCTA AGAGCCCAGA CGGCCAGCGA TGCTGGCGTG 
GGAGTCCGAT CACTTCCCGT GGATTTTAGA TACCCTAACC TAGACTTTGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAGTT GCTAACTCCT CTTCAGCTGA AAATGAAAAT 
TACTGTAAGC ACAGCACAAT TGTCGTCCCT GAAAATGCTG CACGTCAAGG TGCTAATAGA 
ACCTCCTCTC TAGAAAATCA TGACCAAATG TCTGTGAACT GCAGAACGTT ATTCAGTGAG 
TCACCTGGTA AGCTCCAGAT TGAAGTTGTA ACAGATCTTA CAGCAATTAA TGGTCTTTCT 
TATAATAAAA ATCTTGCCAA TGGCAGTTAT GATTTAGCTA ACAGAGACTT TTGCCAAGGA 
AATCAGCTGA ATTACTACAA GCAGGAAATA CCTGTACAAC CAACTACCTC ATATCCCATT 
CAGAATTTAT ACAATTACGA GAACCAGCCC AAGCCCAGCG ATGAATGTAC TCTACTGAGT 
AATAAATACC TTGATGGAAA TGCTAACAAA TCTACCTCAG ATGGAAGTCC CACGACGGCC 
GCAATGCCTG GTACTACCGA AGCTGTTCGA GCGCTCAAGG ACAAGATGGG TTCTGAGCAG 
AGCCCTTGTC CTGGGTACTC CTCGAGGACT CTTGGCCCAA ATCCTGGACT TATTCTGCAG 
GCTTTGACCC TTTCAAATGC TAGTGATGGA TTTAACCTGG AGCGGCTTGA AATGCTTGGT 
GACTCCTTTT TAAAGCATGC CATCACCACA TATCTATTTT GCACTTATCC TGATGCTCAT 
GAGGGCCGCC TTTCATATAT GAGAAGCAAA AAGGTCAGCA ACTGTAATCT GTATCGGCTT 
GGAAAAAAGA AGGGACTGCC CAGCCGCATG GTGGTATCAA TATTTGATCC CCCTGTGAAT 
TGGCTTCCTC CTGGTTATGT AGTAAACCAA GACAAAAGTA ACGCAGATAA ATGGGAAAAA 
GATGAAATGA CAAAAGACTG CATGTTGGCT AATGGCAAGC TGGACGAGGA CTTTGAGGAG 
GACGACGAGG AGGAGGAGGA TCTAATGTGG AGGGCTCCGA AGGAGGACGC CGATTATGAA 
GATGATTTCC TGGAGTATGA TCAGGAACAT ATCAAATTTA TAGATAACAT GTTAATGGGA 
TCAGGAGCTT TTGTAAAGAA AATTTCTCTC TCTCCTTTTT CAACCACTGA TTCTGCATAC 
GAATGGAAAA TGCCCAAAAA ATCCTCCTTA GGTAGTATGC CATTTTCATC AGATTTTGAG 
GATTTTGACT ATAGCTCTTG GGATGCCATG TGCTATCTGG ATCCTAGCAA AGCTGTGGAA 
GAGGATGACT TTGTGGTGGG GTTCTGGAAT CCATCAGAAG AAAACTGTGG TGTTGACACG 
GGGAAACAGT CCATTTCTTA CGACTTGCAC ACTGAGCAGT GTATTGCGGA CAAAAGCATA 
GCCGACTGCG TGGAAGCCCT GCTGGGCTGC TATTTAACCA GCTGCGGCGA GAGGGCTGCT 
CAGCTCTTCC TCTGCTCACT GGGGCTCAAG GTGCTCCCGG TCATGAAAAG GACCGATCGG 
GAAAAGACCA TGTGCCCTCC TAGGGAGAAT TTCAGCAGCC AACAAAAGAA CCTTTCAGGG 
GGCCGCGCTG CTGCCTCTGT GGCCAGTTTA CGTCCCTCTG TGTTGAAAGA CTTGGAGTAT 
GGCTGTTTGA AGATTCCACC GAGGTGTATG TTTGATCATC CGGACGCAGA TAAAACACTG 
AATCACCTTA TATCGGGTTT TGAGAATTTT GAAAAGAAAA TCAACTACAG ATTCAAGAAT 
AAGGCTTATC TTCTACAGGC TTTTACCCAT GCCTCCTACC ACTACAATAC TATTACTGAT 
TGTTACCAGC GCCTGGAGTT CCTGGGAGAT GCGATCTTGG ACTACCTCAT AACCAAGCAC 
CTTTACGAAG ACCCGCGGCA GCACTCCCCG GGCGTCCTGA CCGACCTGCG GTCCGCACTG 
GTCAACAACA CCATCTTCGC GTCGCTGGCG GTCAAGTACG ACTACCACAA GTACTTCAAA 
GCTGTCTCCC CTGAGCTCTT CCATGTCATT GATGACTTCG TGCAGTTCCA GCTGGAGAAG 
AATGAGATGC AGGGCATGGA CTCCGAGCTT AGGAGGTCTG AGGAGGATGA AGAGAAAGAA 
GAGGACATTG AAGTTCCAAA GGCCATGGGG GACATTTTTG AGTCGCTTGC TGGTGCCATT 
TACATGGATA GTGGGATGTC ACTGGAGATG GTTTGGCAGG TGTACTATCC TATGATGCGG 
CCGCTGATAG AAAAATTTTC TGCAAACGTG CCCCGTTCCC CTGTGCGAGA ATTGCTTGAA 
ATGGAACCAG AAACTGCCAA ATTTAGCCCG GCTGAGAGGA CGTATGACGG CAAGGTCAGA 
GTCACGGTGG AAGTGGTAGG GAAGGGGAAA TTTAAAGGCG TCGGCCGGAG CTACAGGATC 
GCCAAGTCTG CAGCAGCAAG AAGAGCCCTA CGCAGCCTCA AAGCCAATCA ACCTCAGGTT 
CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971615.1
CDS	357..6128
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYGRLLVELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIEPVVDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAARQGANRTSSLENHDQMSVNCRTLFSESPGKLQIEVVTDLTAINGLSYNKNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANKSTSDGSPTTAAMPGTTEAVRALKDKMGSEQSPCPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNADKWEKDEMTKDCMLANGKLDEDFEEDDEEEEDLMWRAPKEDADYEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVMKRTDREKTMCPPRENFSSQQKNLSGGRAAASVASLRPSVLKDLEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEMVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_014116140.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116140.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCC 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAAC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCTCTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCGTGTCCT 
CGTATTTTGG GACTCACTGC TTCCATTTTA AATGGGAAAT GCGATCCCGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTG AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTATTGG ACAGATACAC TTCTCAACCC TGTGAGATTG TGGTGGACTG TGGGCCATTC 
ACGGACAGAA GTGGCCTCTA TGGCAGACTG CTGGTGGAGC TAGAGGAAGC ACTTAACTTT 
ATCAACGACT GTAACATATC TGTGCATTCG AAAGAAAGAG ATTCTACTCT AATTTCTAAA 
CAGATACTGT CAGACTGTCG TGCGGTATTG GTAGTCCTGG GCCCCTGGTG TGCAGATAAA 
GTAGCTGGGA TGATGGTAAG AGAACTGCAA AAGTATATCA AACATGAACA GGAAGAGCTG 
CACAGGAAAT TTCTATTGTT TACAGACACC TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CTCCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATAAAACTG 
CTTGAAATCC TACGCAAATA TAAACCGTAC GAGCGACAGC AGTTTGAAAG TGTCGAGTGG 
TATAATAATA GGAATCAGGA TAATTATGTG TCTTGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACCAACT TTCCTTCTCC ATTTACCAAT 
ATTTTATGTG GAATCATTTT TGTGGAAAGA AGATACACGG CTGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTTCTCAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGATT TGATTTGCCC 
ACAGAGTATC GATCCTATGT TCAATCTAAG GGAAGAGCAA GGGCACCGAT CTCTAATTAT 
ATAATGTTAG CAGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTAAG AAACAAGTGT TCCAAGTCAG TTGACACTGG TGAGACTGAC 
ATTGAACCTG TTGTGGATGA TGACGACGTG TTCCCACCAT ACGTGTTGAG GCCTGATGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGTA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTCCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCC GAAAGAGTCG TAGCTCTCAT TTGCTGTGAA 
AAACTGCACA AAATTGGTGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACCGTT 
AAATATGAAG AAGAGCTTGA TTTACATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACAA AACGAAGACA GTGCTACCCA AAAGCAATTC CAGAATGTTT AAGGGATAGC 
TATCCCAAAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTCT GACGACGCCC 
TTACCTGATG AACTCAACTT CAGAAGGCGC AAGCTCTATC CCCCTGAGGA TACCACACGA 
TGCTTTGGAA TACTAACAGC CAAACCTATA CCTCAGATCC CGCACTTTCC TGTGTACACG 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAC ATTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCCACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TGAATGACTC CAGCACTTTG GACATTGACT TTAAATTCAT GGAAGATATT 
GAGAAATCTG AAGCTCGCAT AGGCATTCCC AGTACCAAGT ATTCAAAAGA AACACCTTTT 
GTTTTTAAAT TAGAAGATTA CCAGGATGCA GTTATCATTC CAAGATATCG TAATTTTGAT 
CAGCCTCATC GATTCTATGT AGCTGATGTA TACACTGATC TTACCCCACT GAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAATACAA CCTTGACCTG 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGTC ATTTGAATCA GAAGGGGAAA GCGCTTCCTC TTAGCAGTGC TGAAAAGAGA 
AAAGCCAAGT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAGCT CTGTGCTATA 
CACCCAATTC CAGCGTCACT GTGGCGAAAA GCAGTCTGTC TCCCTAGTAT ACTCTATCGC 
CTTCACTGCC TTTTGACCGC AGAGGAGCTA AGAGCCCAGA CGGCCAGCGA TGCTGGCGTG 
GGAGTCCGAT CACTTCCCGT GGATTTTAGA TACCCTAACC TAGACTTTGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAGTT GCTAACTCCT CTTCAGCTGA AAATGAAAAT 
TACTGTAAGC ACAGCACAAT TGTCGTCCCT GAAAATGCTG CACGTCAAGG TGCTAATAGA 
ACCTCCTCTC TAGAAAATCA TGACCAAATG TCTGTGAACT GCAGAACGTT ATTCAGTGAG 
TCACCTGGTA AGCTCCAGAT TGAAGTTGTA ACAGATCTTA CAGCAATTAA TGGTCTTTCT 
TATAATAAAA ATCTTGCCAA TGGCAGTTAT GATTTAGCTA ACAGAGACTT TTGCCAAGGA 
AATCAGCTGA ATTACTACAA GCAGGAAATA CCTGTACAAC CAACTACCTC ATATCCCATT 
CAGAATTTAT ACAATTACGA GAACCAGCCC AAGCCCAGCG ATGAATGTAC TCTACTGAGT 
AATAAATACC TTGATGGAAA TGCTAACAAA TCTACCTCAG ATGGAAGTCC CACGACGGCC 
GCAATGCCTG GTACTACCGA AGCTGTTCGA GCGCTCAAGG ACAAGATGGG TTCTGAGCAG 
AGCCCTTGTC CTGGGTACTC CTCGAGGACT CTTGGCCCAA ATCCTGGACT TATTCTGCAG 
GCTTTGACCC TTTCAAATGC TAGTGATGGA TTTAACCTGG AGCGGCTTGA AATGCTTGGT 
GACTCCTTTT TAAAGCATGC CATCACCACA TATCTATTTT GCACTTATCC TGATGCTCAT 
GAGGGCCGCC TTTCATATAT GAGAAGCAAA AAGGTCAGCA ACTGTAATCT GTATCGGCTT 
GGAAAAAAGA AGGGACTGCC CAGCCGCATG GTGGTATCAA TATTTGATCC CCCTGTGAAT 
TGGCTTCCTC CTGGTTATGT AGTAAACCAA GACAAAAGTA ACGCAGATAA ATGGGAAAAA 
GATGAAATGA CAAAAGACTG CATGTTGGCT AATGGCAAGC TGGACGAGGA CTTTGAGGAG 
GACGACGAGG AGGAGGAGGA TCTAATGTGG AGGGCTCCGA AGGAGGACGC CGATTATGAA 
GATGATTTCC TGGAGTATGA TCAGGAACAT ATCAAATTTA TAGATAACAT GTTAATGGGA 
TCAGGAGCTT TTGTAAAGAA AATTTCTCTC TCTCCTTTTT CAACCACTGA TTCTGCATAC 
GAATGGAAAA TGCCCAAAAA ATCCTCCTTA GGTAGTATGC CATTTTCATC AGATTTTGAG 
GATTTTGACT ATAGCTCTTG GGATGCCATG TGCTATCTGG ATCCTAGCAA AGCTGTGGAA 
GAGGATGACT TTGTGGTGGG GTTCTGGAAT CCATCAGAAG AAAACTGTGG TGTTGACACG 
GGGAAACAGT CCATTTCTTA CGACTTGCAC ACTGAGCAGT GTATTGCGGA CAAAAGCATA 
GCCGACTGCG TGGAAGCCCT GCTGGGCTGC TATTTAACCA GCTGCGGCGA GAGGGCTGCT 
CAGCTCTTCC TCTGCTCACT GGGGCTCAAG GTGCTCCCGG TCATGAAAAG GACCGATCGG 
GAAAAGACCA TGTGCCCTCC TAGGGAGAAT TTCAGCAGCC AACAAAAGAA CCTTTCAGGG 
GGCCGCGCTG CTGCCTCTGT GGCCAGTTTA CGTCCCTCTG TGTTGAAAGA CTTGGAGTAT 
GGCTGTTTGA AGATTCCACC GAGGTGTATG TTTGATCATC CGGACGCAGA TAAAACACTG 
AATCACCTTA TATCGGGTTT TGAGAATTTT GAAAAGAAAA TCAACTACAG ATTCAAGAAT 
AAGGCTTATC TTCTACAGGC TTTTACCCAT GCCTCCTACC ACTACAATAC TATTACTGAT 
TGTTACCAGC GCCTGGAGTT CCTGGGAGAT GCGATCTTGG ACTACCTCAT AACCAAGCAC 
CTTTACGAAG ACCCGCGGCA GCACTCCCCG GGCGTCCTGA CCGACCTGCG GTCCGCACTG 
GTCAACAACA CCATCTTCGC GTCGCTGGCG GTCAAGTACG ACTACCACAA GTACTTCAAA 
GCTGTCTCCC CTGAGCTCTT CCATGTCATT GATGACTTCG TGCAGTTCCA GCTGGAGAAG 
AATGAGATGC AGGGCATGGA CTCCGAGCTT AGGAGGTCTG AGGAGGATGA AGAGAAAGAA 
GAGGACATTG AAGTTCCAAA GGCCATGGGG GACATTTTTG AGTCGCTTGC TGGTGCCATT 
TACATGGATA GTGGGATGTC ACTGGAGATG GTTTGGCAGG TGTACTATCC TATGATGCGG 
CCGCTGATAG AAAAATTTTC TGCAAACGTG CCCCGTTCCC CTGTGCGAGA ATTGCTTGAA 
ATGGAACCAG AAACTGCCAA ATTTAGCCCG GCTGAGAGGA CGTATGACGG CAAGGTCAGA 
GTCACGGTGG AAGTGGTAGG GAAGGGGAAA TTTAAAGGCG TCGGCCGGAG CTACAGGATC 
GCCAAGTCTG CAGCAGCAAG AAGAGCCCTA CGCAGCCTCA AAGCCAATCA ACCTCAGGTT 
CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_022422353.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022278061.1 Canis lupus familiaris dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.

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CloneID	OCe38040
Clone ID Related Accession (Same CDS sequence)	XM_863433.4 , XM_014116140.2 , XM_022422353.1 , XM_863433.5 , XM_014116140.1
Accession Version	XM_022422353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	endoribonuclease Dicer
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCC 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAAC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCTCTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCGTGTCCT 
CGTATTTTGG GACTCACTGC TTCCATTTTA AATGGGAAAT GCGATCCCGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTG AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTATTGG ACAGATACAC TTCTCAACCC TGTGAGATTG TGGTGGACTG TGGGCCATTC 
ACGGACAGAA GTGGCCTCTA TGGCAGACTG CTGGTGGAGC TAGAGGAAGC ACTTAACTTT 
ATCAACGACT GTAACATATC TGTGCATTCG AAAGAAAGAG ATTCTACTCT AATTTCTAAA 
CAGATACTGT CAGACTGTCG TGCGGTATTG GTAGTCCTGG GCCCCTGGTG TGCAGATAAA 
GTAGCTGGGA TGATGGTAAG AGAACTGCAA AAGTATATCA AACATGAACA GGAAGAGCTG 
CACAGGAAAT TTCTATTGTT TACAGACACC TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CTCCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATAAAACTG 
CTTGAAATCC TACGCAAATA TAAACCGTAC GAGCGACAGC AGTTTGAAAG TGTCGAGTGG 
TATAATAATA GGAATCAGGA TAATTATGTG TCTTGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACCAACT TTCCTTCTCC ATTTACCAAT 
ATTTTATGTG GAATCATTTT TGTGGAAAGA AGATACACGG CTGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTTCTCAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGATT TGATTTGCCC 
ACAGAGTATC GATCCTATGT TCAATCTAAG GGAAGAGCAA GGGCACCGAT CTCTAATTAT 
ATAATGTTAG CAGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTAAG AAACAAGTGT TCCAAGTCAG TTGACACTGG TGAGACTGAC 
ATTGAACCTG TTGTGGATGA TGACGACGTG TTCCCACCAT ACGTGTTGAG GCCTGATGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGTA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTCCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCC GAAAGAGTCG TAGCTCTCAT TTGCTGTGAA 
AAACTGCACA AAATTGGTGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACCGTT 
AAATATGAAG AAGAGCTTGA TTTACATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACAA AACGAAGACA GTGCTACCCA AAAGCAATTC CAGAATGTTT AAGGGATAGC 
TATCCCAAAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTCT GACGACGCCC 
TTACCTGATG AACTCAACTT CAGAAGGCGC AAGCTCTATC CCCCTGAGGA TACCACACGA 
TGCTTTGGAA TACTAACAGC CAAACCTATA CCTCAGATCC CGCACTTTCC TGTGTACACG 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAC ATTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCCACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TGAATGACTC CAGCACTTTG GACATTGACT TTAAATTCAT GGAAGATATT 
GAGAAATCTG AAGCTCGCAT AGGCATTCCC AGTACCAAGT ATTCAAAAGA AACACCTTTT 
GTTTTTAAAT TAGAAGATTA CCAGGATGCA GTTATCATTC CAAGATATCG TAATTTTGAT 
CAGCCTCATC GATTCTATGT AGCTGATGTA TACACTGATC TTACCCCACT GAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAATACAA CCTTGACCTG 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGTC ATTTGAATCA GAAGGGGAAA GCGCTTCCTC TTAGCAGTGC TGAAAAGAGA 
AAAGCCAAGT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAGCT CTGTGCTATA 
CACCCAATTC CAGCGTCACT GTGGCGAAAA GCAGTCTGTC TCCCTAGTAT ACTCTATCGC 
CTTCACTGCC TTTTGACCGC AGAGGAGCTA AGAGCCCAGA CGGCCAGCGA TGCTGGCGTG 
GGAGTCCGAT CACTTCCCGT GGATTTTAGA TACCCTAACC TAGACTTTGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAGTT GCTAACTCCT CTTCAGCTGA AAATGAAAAT 
TACTGTAAGC ACAGCACAAT TGTCGTCCCT GAAAATGCTG CACGTCAAGG TGCTAATAGA 
ACCTCCTCTC TAGAAAATCA TGACCAAATG TCTGTGAACT GCAGAACGTT ATTCAGTGAG 
TCACCTGGTA AGCTCCAGAT TGAAGTTGTA ACAGATCTTA CAGCAATTAA TGGTCTTTCT 
TATAATAAAA ATCTTGCCAA TGGCAGTTAT GATTTAGCTA ACAGAGACTT TTGCCAAGGA 
AATCAGCTGA ATTACTACAA GCAGGAAATA CCTGTACAAC CAACTACCTC ATATCCCATT 
CAGAATTTAT ACAATTACGA GAACCAGCCC AAGCCCAGCG ATGAATGTAC TCTACTGAGT 
AATAAATACC TTGATGGAAA TGCTAACAAA TCTACCTCAG ATGGAAGTCC CACGACGGCC 
GCAATGCCTG GTACTACCGA AGCTGTTCGA GCGCTCAAGG ACAAGATGGG TTCTGAGCAG 
AGCCCTTGTC CTGGGTACTC CTCGAGGACT CTTGGCCCAA ATCCTGGACT TATTCTGCAG 
GCTTTGACCC TTTCAAATGC TAGTGATGGA TTTAACCTGG AGCGGCTTGA AATGCTTGGT 
GACTCCTTTT TAAAGCATGC CATCACCACA TATCTATTTT GCACTTATCC TGATGCTCAT 
GAGGGCCGCC TTTCATATAT GAGAAGCAAA AAGGTCAGCA ACTGTAATCT GTATCGGCTT 
GGAAAAAAGA AGGGACTGCC CAGCCGCATG GTGGTATCAA TATTTGATCC CCCTGTGAAT 
TGGCTTCCTC CTGGTTATGT AGTAAACCAA GACAAAAGTA ACGCAGATAA ATGGGAAAAA 
GATGAAATGA CAAAAGACTG CATGTTGGCT AATGGCAAGC TGGACGAGGA CTTTGAGGAG 
GACGACGAGG AGGAGGAGGA TCTAATGTGG AGGGCTCCGA AGGAGGACGC CGATTATGAA 
GATGATTTCC TGGAGTATGA TCAGGAACAT ATCAAATTTA TAGATAACAT GTTAATGGGA 
TCAGGAGCTT TTGTAAAGAA AATTTCTCTC TCTCCTTTTT CAACCACTGA TTCTGCATAC 
GAATGGAAAA TGCCCAAAAA ATCCTCCTTA GGTAGTATGC CATTTTCATC AGATTTTGAG 
GATTTTGACT ATAGCTCTTG GGATGCCATG TGCTATCTGG ATCCTAGCAA AGCTGTGGAA 
GAGGATGACT TTGTGGTGGG GTTCTGGAAT CCATCAGAAG AAAACTGTGG TGTTGACACG 
GGGAAACAGT CCATTTCTTA CGACTTGCAC ACTGAGCAGT GTATTGCGGA CAAAAGCATA 
GCCGACTGCG TGGAAGCCCT GCTGGGCTGC TATTTAACCA GCTGCGGCGA GAGGGCTGCT 
CAGCTCTTCC TCTGCTCACT GGGGCTCAAG GTGCTCCCGG TCATGAAAAG GACCGATCGG 
GAAAAGACCA TGTGCCCTCC TAGGGAGAAT TTCAGCAGCC AACAAAAGAA CCTTTCAGGG 
GGCCGCGCTG CTGCCTCTGT GGCCAGTTTA CGTCCCTCTG TGTTGAAAGA CTTGGAGTAT 
GGCTGTTTGA AGATTCCACC GAGGTGTATG TTTGATCATC CGGACGCAGA TAAAACACTG 
AATCACCTTA TATCGGGTTT TGAGAATTTT GAAAAGAAAA TCAACTACAG ATTCAAGAAT 
AAGGCTTATC TTCTACAGGC TTTTACCCAT GCCTCCTACC ACTACAATAC TATTACTGAT 
TGTTACCAGC GCCTGGAGTT CCTGGGAGAT GCGATCTTGG ACTACCTCAT AACCAAGCAC 
CTTTACGAAG ACCCGCGGCA GCACTCCCCG GGCGTCCTGA CCGACCTGCG GTCCGCACTG 
GTCAACAACA CCATCTTCGC GTCGCTGGCG GTCAAGTACG ACTACCACAA GTACTTCAAA 
GCTGTCTCCC CTGAGCTCTT CCATGTCATT GATGACTTCG TGCAGTTCCA GCTGGAGAAG 
AATGAGATGC AGGGCATGGA CTCCGAGCTT AGGAGGTCTG AGGAGGATGA AGAGAAAGAA 
GAGGACATTG AAGTTCCAAA GGCCATGGGG GACATTTTTG AGTCGCTTGC TGGTGCCATT 
TACATGGATA GTGGGATGTC ACTGGAGATG GTTTGGCAGG TGTACTATCC TATGATGCGG 
CCGCTGATAG AAAAATTTTC TGCAAACGTG CCCCGTTCCC CTGTGCGAGA ATTGCTTGAA 
ATGGAACCAG AAACTGCCAA ATTTAGCCCG GCTGAGAGGA CGTATGACGG CAAGGTCAGA 
GTCACGGTGG AAGTGGTAGG GAAGGGGAAA TTTAAAGGCG TCGGCCGGAG CTACAGGATC 
GCCAAGTCTG CAGCAGCAAG AAGAGCCCTA CGCAGCCTCA AAGCCAATCA ACCTCAGGTT 
CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022278061.1
CDS	1..5772
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYGRLLVELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIEPVVDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAARQGANRTSSLENHDQMSVNCRTLFSESPGKLQIEVVTDLTAINGLSYNKNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANKSTSDGSPTTAAMPGTTEAVRALKDKMGSEQSPCPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNADKWEKDEMTKDCMLANGKLDEDFEEDDEEEEDLMWRAPKEDADYEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVMKRTDREKTMCPPRENFSSQQKNLSGGRAAASVASLRPSVLKDLEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEMVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_022422353.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022422353.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCC 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAAC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCTCTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCGTGTCCT 
CGTATTTTGG GACTCACTGC TTCCATTTTA AATGGGAAAT GCGATCCCGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTG AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTATTGG ACAGATACAC TTCTCAACCC TGTGAGATTG TGGTGGACTG TGGGCCATTC 
ACGGACAGAA GTGGCCTCTA TGGCAGACTG CTGGTGGAGC TAGAGGAAGC ACTTAACTTT 
ATCAACGACT GTAACATATC TGTGCATTCG AAAGAAAGAG ATTCTACTCT AATTTCTAAA 
CAGATACTGT CAGACTGTCG TGCGGTATTG GTAGTCCTGG GCCCCTGGTG TGCAGATAAA 
GTAGCTGGGA TGATGGTAAG AGAACTGCAA AAGTATATCA AACATGAACA GGAAGAGCTG 
CACAGGAAAT TTCTATTGTT TACAGACACC TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CTCCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATAAAACTG 
CTTGAAATCC TACGCAAATA TAAACCGTAC GAGCGACAGC AGTTTGAAAG TGTCGAGTGG 
TATAATAATA GGAATCAGGA TAATTATGTG TCTTGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACCAACT TTCCTTCTCC ATTTACCAAT 
ATTTTATGTG GAATCATTTT TGTGGAAAGA AGATACACGG CTGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTTCTCAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGATT TGATTTGCCC 
ACAGAGTATC GATCCTATGT TCAATCTAAG GGAAGAGCAA GGGCACCGAT CTCTAATTAT 
ATAATGTTAG CAGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTAAG AAACAAGTGT TCCAAGTCAG TTGACACTGG TGAGACTGAC 
ATTGAACCTG TTGTGGATGA TGACGACGTG TTCCCACCAT ACGTGTTGAG GCCTGATGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGTA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTCCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCC GAAAGAGTCG TAGCTCTCAT TTGCTGTGAA 
AAACTGCACA AAATTGGTGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACCGTT 
AAATATGAAG AAGAGCTTGA TTTACATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACAA AACGAAGACA GTGCTACCCA AAAGCAATTC CAGAATGTTT AAGGGATAGC 
TATCCCAAAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTCT GACGACGCCC 
TTACCTGATG AACTCAACTT CAGAAGGCGC AAGCTCTATC CCCCTGAGGA TACCACACGA 
TGCTTTGGAA TACTAACAGC CAAACCTATA CCTCAGATCC CGCACTTTCC TGTGTACACG 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAC ATTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCCACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TGAATGACTC CAGCACTTTG GACATTGACT TTAAATTCAT GGAAGATATT 
GAGAAATCTG AAGCTCGCAT AGGCATTCCC AGTACCAAGT ATTCAAAAGA AACACCTTTT 
GTTTTTAAAT TAGAAGATTA CCAGGATGCA GTTATCATTC CAAGATATCG TAATTTTGAT 
CAGCCTCATC GATTCTATGT AGCTGATGTA TACACTGATC TTACCCCACT GAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAATACAA CCTTGACCTG 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGTC ATTTGAATCA GAAGGGGAAA GCGCTTCCTC TTAGCAGTGC TGAAAAGAGA 
AAAGCCAAGT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAGCT CTGTGCTATA 
CACCCAATTC CAGCGTCACT GTGGCGAAAA GCAGTCTGTC TCCCTAGTAT ACTCTATCGC 
CTTCACTGCC TTTTGACCGC AGAGGAGCTA AGAGCCCAGA CGGCCAGCGA TGCTGGCGTG 
GGAGTCCGAT CACTTCCCGT GGATTTTAGA TACCCTAACC TAGACTTTGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAGTT GCTAACTCCT CTTCAGCTGA AAATGAAAAT 
TACTGTAAGC ACAGCACAAT TGTCGTCCCT GAAAATGCTG CACGTCAAGG TGCTAATAGA 
ACCTCCTCTC TAGAAAATCA TGACCAAATG TCTGTGAACT GCAGAACGTT ATTCAGTGAG 
TCACCTGGTA AGCTCCAGAT TGAAGTTGTA ACAGATCTTA CAGCAATTAA TGGTCTTTCT 
TATAATAAAA ATCTTGCCAA TGGCAGTTAT GATTTAGCTA ACAGAGACTT TTGCCAAGGA 
AATCAGCTGA ATTACTACAA GCAGGAAATA CCTGTACAAC CAACTACCTC ATATCCCATT 
CAGAATTTAT ACAATTACGA GAACCAGCCC AAGCCCAGCG ATGAATGTAC TCTACTGAGT 
AATAAATACC TTGATGGAAA TGCTAACAAA TCTACCTCAG ATGGAAGTCC CACGACGGCC 
GCAATGCCTG GTACTACCGA AGCTGTTCGA GCGCTCAAGG ACAAGATGGG TTCTGAGCAG 
AGCCCTTGTC CTGGGTACTC CTCGAGGACT CTTGGCCCAA ATCCTGGACT TATTCTGCAG 
GCTTTGACCC TTTCAAATGC TAGTGATGGA TTTAACCTGG AGCGGCTTGA AATGCTTGGT 
GACTCCTTTT TAAAGCATGC CATCACCACA TATCTATTTT GCACTTATCC TGATGCTCAT 
GAGGGCCGCC TTTCATATAT GAGAAGCAAA AAGGTCAGCA ACTGTAATCT GTATCGGCTT 
GGAAAAAAGA AGGGACTGCC CAGCCGCATG GTGGTATCAA TATTTGATCC CCCTGTGAAT 
TGGCTTCCTC CTGGTTATGT AGTAAACCAA GACAAAAGTA ACGCAGATAA ATGGGAAAAA 
GATGAAATGA CAAAAGACTG CATGTTGGCT AATGGCAAGC TGGACGAGGA CTTTGAGGAG 
GACGACGAGG AGGAGGAGGA TCTAATGTGG AGGGCTCCGA AGGAGGACGC CGATTATGAA 
GATGATTTCC TGGAGTATGA TCAGGAACAT ATCAAATTTA TAGATAACAT GTTAATGGGA 
TCAGGAGCTT TTGTAAAGAA AATTTCTCTC TCTCCTTTTT CAACCACTGA TTCTGCATAC 
GAATGGAAAA TGCCCAAAAA ATCCTCCTTA GGTAGTATGC CATTTTCATC AGATTTTGAG 
GATTTTGACT ATAGCTCTTG GGATGCCATG TGCTATCTGG ATCCTAGCAA AGCTGTGGAA 
GAGGATGACT TTGTGGTGGG GTTCTGGAAT CCATCAGAAG AAAACTGTGG TGTTGACACG 
GGGAAACAGT CCATTTCTTA CGACTTGCAC ACTGAGCAGT GTATTGCGGA CAAAAGCATA 
GCCGACTGCG TGGAAGCCCT GCTGGGCTGC TATTTAACCA GCTGCGGCGA GAGGGCTGCT 
CAGCTCTTCC TCTGCTCACT GGGGCTCAAG GTGCTCCCGG TCATGAAAAG GACCGATCGG 
GAAAAGACCA TGTGCCCTCC TAGGGAGAAT TTCAGCAGCC AACAAAAGAA CCTTTCAGGG 
GGCCGCGCTG CTGCCTCTGT GGCCAGTTTA CGTCCCTCTG TGTTGAAAGA CTTGGAGTAT 
GGCTGTTTGA AGATTCCACC GAGGTGTATG TTTGATCATC CGGACGCAGA TAAAACACTG 
AATCACCTTA TATCGGGTTT TGAGAATTTT GAAAAGAAAA TCAACTACAG ATTCAAGAAT 
AAGGCTTATC TTCTACAGGC TTTTACCCAT GCCTCCTACC ACTACAATAC TATTACTGAT 
TGTTACCAGC GCCTGGAGTT CCTGGGAGAT GCGATCTTGG ACTACCTCAT AACCAAGCAC 
CTTTACGAAG ACCCGCGGCA GCACTCCCCG GGCGTCCTGA CCGACCTGCG GTCCGCACTG 
GTCAACAACA CCATCTTCGC GTCGCTGGCG GTCAAGTACG ACTACCACAA GTACTTCAAA 
GCTGTCTCCC CTGAGCTCTT CCATGTCATT GATGACTTCG TGCAGTTCCA GCTGGAGAAG 
AATGAGATGC AGGGCATGGA CTCCGAGCTT AGGAGGTCTG AGGAGGATGA AGAGAAAGAA 
GAGGACATTG AAGTTCCAAA GGCCATGGGG GACATTTTTG AGTCGCTTGC TGGTGCCATT 
TACATGGATA GTGGGATGTC ACTGGAGATG GTTTGGCAGG TGTACTATCC TATGATGCGG 
CCGCTGATAG AAAAATTTTC TGCAAACGTG CCCCGTTCCC CTGTGCGAGA ATTGCTTGAA 
ATGGAACCAG AAACTGCCAA ATTTAGCCCG GCTGAGAGGA CGTATGACGG CAAGGTCAGA 
GTCACGGTGG AAGTGGTAGG GAAGGGGAAA TTTAAAGGCG TCGGCCGGAG CTACAGGATC 
GCCAAGTCTG CAGCAGCAAG AAGAGCCCTA CGCAGCCTCA AAGCCAATCA ACCTCAGGTT 
CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_863433.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_868526.4 Canis lupus familiaris dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.

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CloneID	OCe38040
Clone ID Related Accession (Same CDS sequence)	XM_863433.4 , XM_014116140.2 , XM_022422353.1 , XM_863433.5 , XM_014116140.1
Accession Version	XM_863433.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	endoribonuclease Dicer
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_863433.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCC 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAAC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCTCTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCGTGTCCT 
CGTATTTTGG GACTCACTGC TTCCATTTTA AATGGGAAAT GCGATCCCGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTG AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTATTGG ACAGATACAC TTCTCAACCC TGTGAGATTG TGGTGGACTG TGGGCCATTC 
ACGGACAGAA GTGGCCTCTA TGGCAGACTG CTGGTGGAGC TAGAGGAAGC ACTTAACTTT 
ATCAACGACT GTAACATATC TGTGCATTCG AAAGAAAGAG ATTCTACTCT AATTTCTAAA 
CAGATACTGT CAGACTGTCG TGCGGTATTG GTAGTCCTGG GCCCCTGGTG TGCAGATAAA 
GTAGCTGGGA TGATGGTAAG AGAACTGCAA AAGTATATCA AACATGAACA GGAAGAGCTG 
CACAGGAAAT TTCTATTGTT TACAGACACC TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CTCCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATAAAACTG 
CTTGAAATCC TACGCAAATA TAAACCGTAC GAGCGACAGC AGTTTGAAAG TGTCGAGTGG 
TATAATAATA GGAATCAGGA TAATTATGTG TCTTGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACCAACT TTCCTTCTCC ATTTACCAAT 
ATTTTATGTG GAATCATTTT TGTGGAAAGA AGATACACGG CTGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTTCTCAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGATT TGATTTGCCC 
ACAGAGTATC GATCCTATGT TCAATCTAAG GGAAGAGCAA GGGCACCGAT CTCTAATTAT 
ATAATGTTAG CAGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTAAG AAACAAGTGT TCCAAGTCAG TTGACACTGG TGAGACTGAC 
ATTGAACCTG TTGTGGATGA TGACGACGTG TTCCCACCAT ACGTGTTGAG GCCTGATGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGTA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTCCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCC GAAAGAGTCG TAGCTCTCAT TTGCTGTGAA 
AAACTGCACA AAATTGGTGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACCGTT 
AAATATGAAG AAGAGCTTGA TTTACATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACAA AACGAAGACA GTGCTACCCA AAAGCAATTC CAGAATGTTT AAGGGATAGC 
TATCCCAAAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTCT GACGACGCCC 
TTACCTGATG AACTCAACTT CAGAAGGCGC AAGCTCTATC CCCCTGAGGA TACCACACGA 
TGCTTTGGAA TACTAACAGC CAAACCTATA CCTCAGATCC CGCACTTTCC TGTGTACACG 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAC ATTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCCACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TGAATGACTC CAGCACTTTG GACATTGACT TTAAATTCAT GGAAGATATT 
GAGAAATCTG AAGCTCGCAT AGGCATTCCC AGTACCAAGT ATTCAAAAGA AACACCTTTT 
GTTTTTAAAT TAGAAGATTA CCAGGATGCA GTTATCATTC CAAGATATCG TAATTTTGAT 
CAGCCTCATC GATTCTATGT AGCTGATGTA TACACTGATC TTACCCCACT GAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAATACAA CCTTGACCTG 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGTC ATTTGAATCA GAAGGGGAAA GCGCTTCCTC TTAGCAGTGC TGAAAAGAGA 
AAAGCCAAGT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAGCT CTGTGCTATA 
CACCCAATTC CAGCGTCACT GTGGCGAAAA GCAGTCTGTC TCCCTAGTAT ACTCTATCGC 
CTTCACTGCC TTTTGACCGC AGAGGAGCTA AGAGCCCAGA CGGCCAGCGA TGCTGGCGTG 
GGAGTCCGAT CACTTCCCGT GGATTTTAGA TACCCTAACC TAGACTTTGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAGTT GCTAACTCCT CTTCAGCTGA AAATGAAAAT 
TACTGTAAGC ACAGCACAAT TGTCGTCCCT GAAAATGCTG CACGTCAAGG TGCTAATAGA 
ACCTCCTCTC TAGAAAATCA TGACCAAATG TCTGTGAACT GCAGAACGTT ATTCAGTGAG 
TCACCTGGTA AGCTCCAGAT TGAAGTTGTA ACAGATCTTA CAGCAATTAA TGGTCTTTCT 
TATAATAAAA ATCTTGCCAA TGGCAGTTAT GATTTAGCTA ACAGAGACTT TTGCCAAGGA 
AATCAGCTGA ATTACTACAA GCAGGAAATA CCTGTACAAC CAACTACCTC ATATCCCATT 
CAGAATTTAT ACAATTACGA GAACCAGCCC AAGCCCAGCG ATGAATGTAC TCTACTGAGT 
AATAAATACC TTGATGGAAA TGCTAACAAA TCTACCTCAG ATGGAAGTCC CACGACGGCC 
GCAATGCCTG GTACTACCGA AGCTGTTCGA GCGCTCAAGG ACAAGATGGG TTCTGAGCAG 
AGCCCTTGTC CTGGGTACTC CTCGAGGACT CTTGGCCCAA ATCCTGGACT TATTCTGCAG 
GCTTTGACCC TTTCAAATGC TAGTGATGGA TTTAACCTGG AGCGGCTTGA AATGCTTGGT 
GACTCCTTTT TAAAGCATGC CATCACCACA TATCTATTTT GCACTTATCC TGATGCTCAT 
GAGGGCCGCC TTTCATATAT GAGAAGCAAA AAGGTCAGCA ACTGTAATCT GTATCGGCTT 
GGAAAAAAGA AGGGACTGCC CAGCCGCATG GTGGTATCAA TATTTGATCC CCCTGTGAAT 
TGGCTTCCTC CTGGTTATGT AGTAAACCAA GACAAAAGTA ACGCAGATAA ATGGGAAAAA 
GATGAAATGA CAAAAGACTG CATGTTGGCT AATGGCAAGC TGGACGAGGA CTTTGAGGAG 
GACGACGAGG AGGAGGAGGA TCTAATGTGG AGGGCTCCGA AGGAGGACGC CGATTATGAA 
GATGATTTCC TGGAGTATGA TCAGGAACAT ATCAAATTTA TAGATAACAT GTTAATGGGA 
TCAGGAGCTT TTGTAAAGAA AATTTCTCTC TCTCCTTTTT CAACCACTGA TTCTGCATAC 
GAATGGAAAA TGCCCAAAAA ATCCTCCTTA GGTAGTATGC CATTTTCATC AGATTTTGAG 
GATTTTGACT ATAGCTCTTG GGATGCCATG TGCTATCTGG ATCCTAGCAA AGCTGTGGAA 
GAGGATGACT TTGTGGTGGG GTTCTGGAAT CCATCAGAAG AAAACTGTGG TGTTGACACG 
GGGAAACAGT CCATTTCTTA CGACTTGCAC ACTGAGCAGT GTATTGCGGA CAAAAGCATA 
GCCGACTGCG TGGAAGCCCT GCTGGGCTGC TATTTAACCA GCTGCGGCGA GAGGGCTGCT 
CAGCTCTTCC TCTGCTCACT GGGGCTCAAG GTGCTCCCGG TCATGAAAAG GACCGATCGG 
GAAAAGACCA TGTGCCCTCC TAGGGAGAAT TTCAGCAGCC AACAAAAGAA CCTTTCAGGG 
GGCCGCGCTG CTGCCTCTGT GGCCAGTTTA CGTCCCTCTG TGTTGAAAGA CTTGGAGTAT 
GGCTGTTTGA AGATTCCACC GAGGTGTATG TTTGATCATC CGGACGCAGA TAAAACACTG 
AATCACCTTA TATCGGGTTT TGAGAATTTT GAAAAGAAAA TCAACTACAG ATTCAAGAAT 
AAGGCTTATC TTCTACAGGC TTTTACCCAT GCCTCCTACC ACTACAATAC TATTACTGAT 
TGTTACCAGC GCCTGGAGTT CCTGGGAGAT GCGATCTTGG ACTACCTCAT AACCAAGCAC 
CTTTACGAAG ACCCGCGGCA GCACTCCCCG GGCGTCCTGA CCGACCTGCG GTCCGCACTG 
GTCAACAACA CCATCTTCGC GTCGCTGGCG GTCAAGTACG ACTACCACAA GTACTTCAAA 
GCTGTCTCCC CTGAGCTCTT CCATGTCATT GATGACTTCG TGCAGTTCCA GCTGGAGAAG 
AATGAGATGC AGGGCATGGA CTCCGAGCTT AGGAGGTCTG AGGAGGATGA AGAGAAAGAA 
GAGGACATTG AAGTTCCAAA GGCCATGGGG GACATTTTTG AGTCGCTTGC TGGTGCCATT 
TACATGGATA GTGGGATGTC ACTGGAGATG GTTTGGCAGG TGTACTATCC TATGATGCGG 
CCGCTGATAG AAAAATTTTC TGCAAACGTG CCCCGTTCCC CTGTGCGAGA ATTGCTTGAA 
ATGGAACCAG AAACTGCCAA ATTTAGCCCG GCTGAGAGGA CGTATGACGG CAAGGTCAGA 
GTCACGGTGG AAGTGGTAGG GAAGGGGAAA TTTAAAGGCG TCGGCCGGAG CTACAGGATC 
GCCAAGTCTG CAGCAGCAAG AAGAGCCCTA CGCAGCCTCA AAGCCAATCA ACCTCAGGTT 
CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_868526.4
CDS	431..6202
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYGRLLVELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIEPVVDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAARQGANRTSSLENHDQMSVNCRTLFSESPGKLQIEVVTDLTAINGLSYNKNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANKSTSDGSPTTAAMPGTTEAVRALKDKMGSEQSPCPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNADKWEKDEMTKDCMLANGKLDEDFEEDDEEEEDLMWRAPKEDADYEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVMKRTDREKTMCPPRENFSSQQKNLSGGRAAASVASLRPSVLKDLEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEMVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_863433.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_863433.5

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCC 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAAC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCTCTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCGTGTCCT 
CGTATTTTGG GACTCACTGC TTCCATTTTA AATGGGAAAT GCGATCCCGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTG AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTATTGG ACAGATACAC TTCTCAACCC TGTGAGATTG TGGTGGACTG TGGGCCATTC 
ACGGACAGAA GTGGCCTCTA TGGCAGACTG CTGGTGGAGC TAGAGGAAGC ACTTAACTTT 
ATCAACGACT GTAACATATC TGTGCATTCG AAAGAAAGAG ATTCTACTCT AATTTCTAAA 
CAGATACTGT CAGACTGTCG TGCGGTATTG GTAGTCCTGG GCCCCTGGTG TGCAGATAAA 
GTAGCTGGGA TGATGGTAAG AGAACTGCAA AAGTATATCA AACATGAACA GGAAGAGCTG 
CACAGGAAAT TTCTATTGTT TACAGACACC TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CTCCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATAAAACTG 
CTTGAAATCC TACGCAAATA TAAACCGTAC GAGCGACAGC AGTTTGAAAG TGTCGAGTGG 
TATAATAATA GGAATCAGGA TAATTATGTG TCTTGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACCAACT TTCCTTCTCC ATTTACCAAT 
ATTTTATGTG GAATCATTTT TGTGGAAAGA AGATACACGG CTGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTTCTCAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGATT TGATTTGCCC 
ACAGAGTATC GATCCTATGT TCAATCTAAG GGAAGAGCAA GGGCACCGAT CTCTAATTAT 
ATAATGTTAG CAGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTAAG AAACAAGTGT TCCAAGTCAG TTGACACTGG TGAGACTGAC 
ATTGAACCTG TTGTGGATGA TGACGACGTG TTCCCACCAT ACGTGTTGAG GCCTGATGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGTA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTCCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCC GAAAGAGTCG TAGCTCTCAT TTGCTGTGAA 
AAACTGCACA AAATTGGTGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACCGTT 
AAATATGAAG AAGAGCTTGA TTTACATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACAA AACGAAGACA GTGCTACCCA AAAGCAATTC CAGAATGTTT AAGGGATAGC 
TATCCCAAAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTCT GACGACGCCC 
TTACCTGATG AACTCAACTT CAGAAGGCGC AAGCTCTATC CCCCTGAGGA TACCACACGA 
TGCTTTGGAA TACTAACAGC CAAACCTATA CCTCAGATCC CGCACTTTCC TGTGTACACG 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAC ATTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCCACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TGAATGACTC CAGCACTTTG GACATTGACT TTAAATTCAT GGAAGATATT 
GAGAAATCTG AAGCTCGCAT AGGCATTCCC AGTACCAAGT ATTCAAAAGA AACACCTTTT 
GTTTTTAAAT TAGAAGATTA CCAGGATGCA GTTATCATTC CAAGATATCG TAATTTTGAT 
CAGCCTCATC GATTCTATGT AGCTGATGTA TACACTGATC TTACCCCACT GAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAATACAA CCTTGACCTG 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGTC ATTTGAATCA GAAGGGGAAA GCGCTTCCTC TTAGCAGTGC TGAAAAGAGA 
AAAGCCAAGT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAGCT CTGTGCTATA 
CACCCAATTC CAGCGTCACT GTGGCGAAAA GCAGTCTGTC TCCCTAGTAT ACTCTATCGC 
CTTCACTGCC TTTTGACCGC AGAGGAGCTA AGAGCCCAGA CGGCCAGCGA TGCTGGCGTG 
GGAGTCCGAT CACTTCCCGT GGATTTTAGA TACCCTAACC TAGACTTTGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAGTT GCTAACTCCT CTTCAGCTGA AAATGAAAAT 
TACTGTAAGC ACAGCACAAT TGTCGTCCCT GAAAATGCTG CACGTCAAGG TGCTAATAGA 
ACCTCCTCTC TAGAAAATCA TGACCAAATG TCTGTGAACT GCAGAACGTT ATTCAGTGAG 
TCACCTGGTA AGCTCCAGAT TGAAGTTGTA ACAGATCTTA CAGCAATTAA TGGTCTTTCT 
TATAATAAAA ATCTTGCCAA TGGCAGTTAT GATTTAGCTA ACAGAGACTT TTGCCAAGGA 
AATCAGCTGA ATTACTACAA GCAGGAAATA CCTGTACAAC CAACTACCTC ATATCCCATT 
CAGAATTTAT ACAATTACGA GAACCAGCCC AAGCCCAGCG ATGAATGTAC TCTACTGAGT 
AATAAATACC TTGATGGAAA TGCTAACAAA TCTACCTCAG ATGGAAGTCC CACGACGGCC 
GCAATGCCTG GTACTACCGA AGCTGTTCGA GCGCTCAAGG ACAAGATGGG TTCTGAGCAG 
AGCCCTTGTC CTGGGTACTC CTCGAGGACT CTTGGCCCAA ATCCTGGACT TATTCTGCAG 
GCTTTGACCC TTTCAAATGC TAGTGATGGA TTTAACCTGG AGCGGCTTGA AATGCTTGGT 
GACTCCTTTT TAAAGCATGC CATCACCACA TATCTATTTT GCACTTATCC TGATGCTCAT 
GAGGGCCGCC TTTCATATAT GAGAAGCAAA AAGGTCAGCA ACTGTAATCT GTATCGGCTT 
GGAAAAAAGA AGGGACTGCC CAGCCGCATG GTGGTATCAA TATTTGATCC CCCTGTGAAT 
TGGCTTCCTC CTGGTTATGT AGTAAACCAA GACAAAAGTA ACGCAGATAA ATGGGAAAAA 
GATGAAATGA CAAAAGACTG CATGTTGGCT AATGGCAAGC TGGACGAGGA CTTTGAGGAG 
GACGACGAGG AGGAGGAGGA TCTAATGTGG AGGGCTCCGA AGGAGGACGC CGATTATGAA 
GATGATTTCC TGGAGTATGA TCAGGAACAT ATCAAATTTA TAGATAACAT GTTAATGGGA 
TCAGGAGCTT TTGTAAAGAA AATTTCTCTC TCTCCTTTTT CAACCACTGA TTCTGCATAC 
GAATGGAAAA TGCCCAAAAA ATCCTCCTTA GGTAGTATGC CATTTTCATC AGATTTTGAG 
GATTTTGACT ATAGCTCTTG GGATGCCATG TGCTATCTGG ATCCTAGCAA AGCTGTGGAA 
GAGGATGACT TTGTGGTGGG GTTCTGGAAT CCATCAGAAG AAAACTGTGG TGTTGACACG 
GGGAAACAGT CCATTTCTTA CGACTTGCAC ACTGAGCAGT GTATTGCGGA CAAAAGCATA 
GCCGACTGCG TGGAAGCCCT GCTGGGCTGC TATTTAACCA GCTGCGGCGA GAGGGCTGCT 
CAGCTCTTCC TCTGCTCACT GGGGCTCAAG GTGCTCCCGG TCATGAAAAG GACCGATCGG 
GAAAAGACCA TGTGCCCTCC TAGGGAGAAT TTCAGCAGCC AACAAAAGAA CCTTTCAGGG 
GGCCGCGCTG CTGCCTCTGT GGCCAGTTTA CGTCCCTCTG TGTTGAAAGA CTTGGAGTAT 
GGCTGTTTGA AGATTCCACC GAGGTGTATG TTTGATCATC CGGACGCAGA TAAAACACTG 
AATCACCTTA TATCGGGTTT TGAGAATTTT GAAAAGAAAA TCAACTACAG ATTCAAGAAT 
AAGGCTTATC TTCTACAGGC TTTTACCCAT GCCTCCTACC ACTACAATAC TATTACTGAT 
TGTTACCAGC GCCTGGAGTT CCTGGGAGAT GCGATCTTGG ACTACCTCAT AACCAAGCAC 
CTTTACGAAG ACCCGCGGCA GCACTCCCCG GGCGTCCTGA CCGACCTGCG GTCCGCACTG 
GTCAACAACA CCATCTTCGC GTCGCTGGCG GTCAAGTACG ACTACCACAA GTACTTCAAA 
GCTGTCTCCC CTGAGCTCTT CCATGTCATT GATGACTTCG TGCAGTTCCA GCTGGAGAAG 
AATGAGATGC AGGGCATGGA CTCCGAGCTT AGGAGGTCTG AGGAGGATGA AGAGAAAGAA 
GAGGACATTG AAGTTCCAAA GGCCATGGGG GACATTTTTG AGTCGCTTGC TGGTGCCATT 
TACATGGATA GTGGGATGTC ACTGGAGATG GTTTGGCAGG TGTACTATCC TATGATGCGG 
CCGCTGATAG AAAAATTTTC TGCAAACGTG CCCCGTTCCC CTGTGCGAGA ATTGCTTGAA 
ATGGAACCAG AAACTGCCAA ATTTAGCCCG GCTGAGAGGA CGTATGACGG CAAGGTCAGA 
GTCACGGTGG AAGTGGTAGG GAAGGGGAAA TTTAAAGGCG TCGGCCGGAG CTACAGGATC 
GCCAAGTCTG CAGCAGCAAG AAGAGCCCTA CGCAGCCTCA AAGCCAATCA ACCTCAGGTT 
CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_014116140.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013971615.1 Canis lupus familiaris dicer 1, ribonuclease type III (DICER1), transcript variant X1, mRNA.

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CloneID	OCe38040
Clone ID Related Accession (Same CDS sequence)	XM_863433.4 , XM_014116140.2 , XM_022422353.1 , XM_863433.5 , XM_014116140.1
Accession Version	XM_014116140.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	endoribonuclease Dicer
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCC 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAAC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCTCTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCGTGTCCT 
CGTATTTTGG GACTCACTGC TTCCATTTTA AATGGGAAAT GCGATCCCGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTG AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTATTGG ACAGATACAC TTCTCAACCC TGTGAGATTG TGGTGGACTG TGGGCCATTC 
ACGGACAGAA GTGGCCTCTA TGGCAGACTG CTGGTGGAGC TAGAGGAAGC ACTTAACTTT 
ATCAACGACT GTAACATATC TGTGCATTCG AAAGAAAGAG ATTCTACTCT AATTTCTAAA 
CAGATACTGT CAGACTGTCG TGCGGTATTG GTAGTCCTGG GCCCCTGGTG TGCAGATAAA 
GTAGCTGGGA TGATGGTAAG AGAACTGCAA AAGTATATCA AACATGAACA GGAAGAGCTG 
CACAGGAAAT TTCTATTGTT TACAGACACC TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CTCCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATAAAACTG 
CTTGAAATCC TACGCAAATA TAAACCGTAC GAGCGACAGC AGTTTGAAAG TGTCGAGTGG 
TATAATAATA GGAATCAGGA TAATTATGTG TCTTGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACCAACT TTCCTTCTCC ATTTACCAAT 
ATTTTATGTG GAATCATTTT TGTGGAAAGA AGATACACGG CTGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTTCTCAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGATT TGATTTGCCC 
ACAGAGTATC GATCCTATGT TCAATCTAAG GGAAGAGCAA GGGCACCGAT CTCTAATTAT 
ATAATGTTAG CAGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTAAG AAACAAGTGT TCCAAGTCAG TTGACACTGG TGAGACTGAC 
ATTGAACCTG TTGTGGATGA TGACGACGTG TTCCCACCAT ACGTGTTGAG GCCTGATGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGTA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTCCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCC GAAAGAGTCG TAGCTCTCAT TTGCTGTGAA 
AAACTGCACA AAATTGGTGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACCGTT 
AAATATGAAG AAGAGCTTGA TTTACATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACAA AACGAAGACA GTGCTACCCA AAAGCAATTC CAGAATGTTT AAGGGATAGC 
TATCCCAAAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTCT GACGACGCCC 
TTACCTGATG AACTCAACTT CAGAAGGCGC AAGCTCTATC CCCCTGAGGA TACCACACGA 
TGCTTTGGAA TACTAACAGC CAAACCTATA CCTCAGATCC CGCACTTTCC TGTGTACACG 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAC ATTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCCACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TGAATGACTC CAGCACTTTG GACATTGACT TTAAATTCAT GGAAGATATT 
GAGAAATCTG AAGCTCGCAT AGGCATTCCC AGTACCAAGT ATTCAAAAGA AACACCTTTT 
GTTTTTAAAT TAGAAGATTA CCAGGATGCA GTTATCATTC CAAGATATCG TAATTTTGAT 
CAGCCTCATC GATTCTATGT AGCTGATGTA TACACTGATC TTACCCCACT GAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAATACAA CCTTGACCTG 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGTC ATTTGAATCA GAAGGGGAAA GCGCTTCCTC TTAGCAGTGC TGAAAAGAGA 
AAAGCCAAGT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAGCT CTGTGCTATA 
CACCCAATTC CAGCGTCACT GTGGCGAAAA GCAGTCTGTC TCCCTAGTAT ACTCTATCGC 
CTTCACTGCC TTTTGACCGC AGAGGAGCTA AGAGCCCAGA CGGCCAGCGA TGCTGGCGTG 
GGAGTCCGAT CACTTCCCGT GGATTTTAGA TACCCTAACC TAGACTTTGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAGTT GCTAACTCCT CTTCAGCTGA AAATGAAAAT 
TACTGTAAGC ACAGCACAAT TGTCGTCCCT GAAAATGCTG CACGTCAAGG TGCTAATAGA 
ACCTCCTCTC TAGAAAATCA TGACCAAATG TCTGTGAACT GCAGAACGTT ATTCAGTGAG 
TCACCTGGTA AGCTCCAGAT TGAAGTTGTA ACAGATCTTA CAGCAATTAA TGGTCTTTCT 
TATAATAAAA ATCTTGCCAA TGGCAGTTAT GATTTAGCTA ACAGAGACTT TTGCCAAGGA 
AATCAGCTGA ATTACTACAA GCAGGAAATA CCTGTACAAC CAACTACCTC ATATCCCATT 
CAGAATTTAT ACAATTACGA GAACCAGCCC AAGCCCAGCG ATGAATGTAC TCTACTGAGT 
AATAAATACC TTGATGGAAA TGCTAACAAA TCTACCTCAG ATGGAAGTCC CACGACGGCC 
GCAATGCCTG GTACTACCGA AGCTGTTCGA GCGCTCAAGG ACAAGATGGG TTCTGAGCAG 
AGCCCTTGTC CTGGGTACTC CTCGAGGACT CTTGGCCCAA ATCCTGGACT TATTCTGCAG 
GCTTTGACCC TTTCAAATGC TAGTGATGGA TTTAACCTGG AGCGGCTTGA AATGCTTGGT 
GACTCCTTTT TAAAGCATGC CATCACCACA TATCTATTTT GCACTTATCC TGATGCTCAT 
GAGGGCCGCC TTTCATATAT GAGAAGCAAA AAGGTCAGCA ACTGTAATCT GTATCGGCTT 
GGAAAAAAGA AGGGACTGCC CAGCCGCATG GTGGTATCAA TATTTGATCC CCCTGTGAAT 
TGGCTTCCTC CTGGTTATGT AGTAAACCAA GACAAAAGTA ACGCAGATAA ATGGGAAAAA 
GATGAAATGA CAAAAGACTG CATGTTGGCT AATGGCAAGC TGGACGAGGA CTTTGAGGAG 
GACGACGAGG AGGAGGAGGA TCTAATGTGG AGGGCTCCGA AGGAGGACGC CGATTATGAA 
GATGATTTCC TGGAGTATGA TCAGGAACAT ATCAAATTTA TAGATAACAT GTTAATGGGA 
TCAGGAGCTT TTGTAAAGAA AATTTCTCTC TCTCCTTTTT CAACCACTGA TTCTGCATAC 
GAATGGAAAA TGCCCAAAAA ATCCTCCTTA GGTAGTATGC CATTTTCATC AGATTTTGAG 
GATTTTGACT ATAGCTCTTG GGATGCCATG TGCTATCTGG ATCCTAGCAA AGCTGTGGAA 
GAGGATGACT TTGTGGTGGG GTTCTGGAAT CCATCAGAAG AAAACTGTGG TGTTGACACG 
GGGAAACAGT CCATTTCTTA CGACTTGCAC ACTGAGCAGT GTATTGCGGA CAAAAGCATA 
GCCGACTGCG TGGAAGCCCT GCTGGGCTGC TATTTAACCA GCTGCGGCGA GAGGGCTGCT 
CAGCTCTTCC TCTGCTCACT GGGGCTCAAG GTGCTCCCGG TCATGAAAAG GACCGATCGG 
GAAAAGACCA TGTGCCCTCC TAGGGAGAAT TTCAGCAGCC AACAAAAGAA CCTTTCAGGG 
GGCCGCGCTG CTGCCTCTGT GGCCAGTTTA CGTCCCTCTG TGTTGAAAGA CTTGGAGTAT 
GGCTGTTTGA AGATTCCACC GAGGTGTATG TTTGATCATC CGGACGCAGA TAAAACACTG 
AATCACCTTA TATCGGGTTT TGAGAATTTT GAAAAGAAAA TCAACTACAG ATTCAAGAAT 
AAGGCTTATC TTCTACAGGC TTTTACCCAT GCCTCCTACC ACTACAATAC TATTACTGAT 
TGTTACCAGC GCCTGGAGTT CCTGGGAGAT GCGATCTTGG ACTACCTCAT AACCAAGCAC 
CTTTACGAAG ACCCGCGGCA GCACTCCCCG GGCGTCCTGA CCGACCTGCG GTCCGCACTG 
GTCAACAACA CCATCTTCGC GTCGCTGGCG GTCAAGTACG ACTACCACAA GTACTTCAAA 
GCTGTCTCCC CTGAGCTCTT CCATGTCATT GATGACTTCG TGCAGTTCCA GCTGGAGAAG 
AATGAGATGC AGGGCATGGA CTCCGAGCTT AGGAGGTCTG AGGAGGATGA AGAGAAAGAA 
GAGGACATTG AAGTTCCAAA GGCCATGGGG GACATTTTTG AGTCGCTTGC TGGTGCCATT 
TACATGGATA GTGGGATGTC ACTGGAGATG GTTTGGCAGG TGTACTATCC TATGATGCGG 
CCGCTGATAG AAAAATTTTC TGCAAACGTG CCCCGTTCCC CTGTGCGAGA ATTGCTTGAA 
ATGGAACCAG AAACTGCCAA ATTTAGCCCG GCTGAGAGGA CGTATGACGG CAAGGTCAGA 
GTCACGGTGG AAGTGGTAGG GAAGGGGAAA TTTAAAGGCG TCGGCCGGAG CTACAGGATC 
GCCAAGTCTG CAGCAGCAAG AAGAGCCCTA CGCAGCCTCA AAGCCAATCA ACCTCAGGTT 
CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971615.1
CDS	344..6115
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYGRLLVELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIEPVVDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISVANSSSAENENYCKHSTIVVPENAARQGANRTSSLENHDQMSVNCRTLFSESPGKLQIEVVTDLTAINGLSYNKNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANKSTSDGSPTTAAMPGTTEAVRALKDKMGSEQSPCPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNADKWEKDEMTKDCMLANGKLDEDFEEDDEEEEDLMWRAPKEDADYEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVMKRTDREKTMCPPRENFSSQQKNLSGGRAAASVASLRPSVLKDLEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEMVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_014116140.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dicer 1, ribonuclease type III (DICER1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116140.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA GCCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCC 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG AGACTTCAAC AGAAATGGAA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC TCAACAAGTG TCAGCTGTCA GAACTCATTC AGATCTCAAG 
GTTGGGGAAT ACTCAAACCT AGAAGTAAAT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCTCTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTCTGTG AAAATTGTCC ATCGTGTCCT 
CGTATTTTGG GACTCACTGC TTCCATTTTA AATGGGAAAT GCGATCCCGA GGAATTGGAA 
GAAAAGATTC AGAAACTGGA GAAAATTCTG AAGAGTAATG CTGAAACTGC AACTGACCTG 
GTGGTATTGG ACAGATACAC TTCTCAACCC TGTGAGATTG TGGTGGACTG TGGGCCATTC 
ACGGACAGAA GTGGCCTCTA TGGCAGACTG CTGGTGGAGC TAGAGGAAGC ACTTAACTTT 
ATCAACGACT GTAACATATC TGTGCATTCG AAAGAAAGAG ATTCTACTCT AATTTCTAAA 
CAGATACTGT CAGACTGTCG TGCGGTATTG GTAGTCCTGG GCCCCTGGTG TGCAGATAAA 
GTAGCTGGGA TGATGGTAAG AGAACTGCAA AAGTATATCA AACATGAACA GGAAGAGCTG 
CACAGGAAAT TTCTATTGTT TACAGACACC TTCCTAAGGA AAATACATGC ACTATGTGAA 
GAGCACTTCT CTCCTGCCTC ACTTGACCTG AAATTTGTAA CTCCTAAAGT AATAAAACTG 
CTTGAAATCC TACGCAAATA TAAACCGTAC GAGCGACAGC AGTTTGAAAG TGTCGAGTGG 
TATAATAATA GGAATCAGGA TAATTATGTG TCTTGGAGTG ATTCTGAGGA TGATGATGAG 
GATGAAGAAA TTGAAGAAAA AGAGAAGCCA GAGACCAACT TTCCTTCTCC ATTTACCAAT 
ATTTTATGTG GAATCATTTT TGTGGAAAGA AGATACACGG CTGTTGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TTGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCAGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAGACCA ACCTTCTCAT TGCAACAAGT 
ATTGTGGAAG AGGGTGTTGA TATACCAAAA TGCAACTTGG TGGTTCGATT TGATTTGCCC 
ACAGAGTATC GATCCTATGT TCAATCTAAG GGAAGAGCAA GGGCACCGAT CTCTAATTAT 
ATAATGTTAG CAGATACAGA CAAAATAAAA AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTAAG AAACAAGTGT TCCAAGTCAG TTGACACTGG TGAGACTGAC 
ATTGAACCTG TTGTGGATGA TGACGACGTG TTCCCACCAT ACGTGTTGAG GCCTGATGAT 
GGTGGTCCAC GAGTCACAAT CAACACGGCC ATTGGACACA TCAATAGATA CTGTGCTAGA 
TTACCAAGTG ATCCGTTTAC TCATCTAGCT CCTAAATGTA GAACCCGAGA GTTGCCTGAT 
GGTACATTTT ATTCAACTCT TTATCTGCCA ATTAACTCAC CTCTCCGAGC CTCCATTGTT 
GGTCCACCAA TGAGCTGTGT ACGATTGGCC GAAAGAGTCG TAGCTCTCAT TTGCTGTGAA 
AAACTGCACA AAATTGGTGA ACTGGATGAC CATTTGATGC CAGTTGGGAA AGAGACCGTT 
AAATATGAAG AAGAGCTTGA TTTACATGAT GAAGAAGAGA CCAGTGTTCC AGGAAGACCA 
GGTTCCACAA AACGAAGACA GTGCTACCCA AAAGCAATTC CAGAATGTTT AAGGGATAGC 
TATCCCAAAC CTGATCAGCC CTGTTACCTG TATGTGATAG GAATGGTTCT GACGACGCCC 
TTACCTGATG AACTCAACTT CAGAAGGCGC AAGCTCTATC CCCCTGAGGA TACCACACGA 
TGCTTTGGAA TACTAACAGC CAAACCTATA CCTCAGATCC CGCACTTTCC TGTGTACACG 
CGCTCTGGAG AGGTTACCAT ATCCATTGAG TTGAAGAAGT CTGGTTTCAC ATTGTCTCTA 
CAAATGCTTG AGTTGATTAC AAGACTTCAC CAGTATATAT TCTCACATAT TCTTCGGCTT 
GAAAAACCTG CACTAGAATT TAAACCCACA GACGCTGATT CAGCATACTG TGTTCTACCT 
CTTAATGTTG TGAATGACTC CAGCACTTTG GACATTGACT TTAAATTCAT GGAAGATATT 
GAGAAATCTG AAGCTCGCAT AGGCATTCCC AGTACCAAGT ATTCAAAAGA AACACCTTTT 
GTTTTTAAAT TAGAAGATTA CCAGGATGCA GTTATCATTC CAAGATATCG TAATTTTGAT 
CAGCCTCATC GATTCTATGT AGCTGATGTA TACACTGATC TTACCCCACT GAGTAAATTT 
CCTTCCCCTG AGTATGAAAC TTTTGCAGAA TATTATAAAA CAAAATACAA CCTTGACCTG 
ACCAATCTCA ACCAGCCACT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACACCTCGTC ATTTGAATCA GAAGGGGAAA GCGCTTCCTC TTAGCAGTGC TGAAAAGAGA 
AAAGCCAAGT GGGAAAGTCT GCAGAATAAA CAGATACTGG TTCCAGAGCT CTGTGCTATA 
CACCCAATTC CAGCGTCACT GTGGCGAAAA GCAGTCTGTC TCCCTAGTAT ACTCTATCGC 
CTTCACTGCC TTTTGACCGC AGAGGAGCTA AGAGCCCAGA CGGCCAGCGA TGCTGGCGTG 
GGAGTCCGAT CACTTCCCGT GGATTTTAGA TACCCTAACC TAGACTTTGG GTGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCAGTT GCTAACTCCT CTTCAGCTGA AAATGAAAAT 
TACTGTAAGC ACAGCACAAT TGTCGTCCCT GAAAATGCTG CACGTCAAGG TGCTAATAGA 
ACCTCCTCTC TAGAAAATCA TGACCAAATG TCTGTGAACT GCAGAACGTT ATTCAGTGAG 
TCACCTGGTA AGCTCCAGAT TGAAGTTGTA ACAGATCTTA CAGCAATTAA TGGTCTTTCT 
TATAATAAAA ATCTTGCCAA TGGCAGTTAT GATTTAGCTA ACAGAGACTT TTGCCAAGGA 
AATCAGCTGA ATTACTACAA GCAGGAAATA CCTGTACAAC CAACTACCTC ATATCCCATT 
CAGAATTTAT ACAATTACGA GAACCAGCCC AAGCCCAGCG ATGAATGTAC TCTACTGAGT 
AATAAATACC TTGATGGAAA TGCTAACAAA TCTACCTCAG ATGGAAGTCC CACGACGGCC 
GCAATGCCTG GTACTACCGA AGCTGTTCGA GCGCTCAAGG ACAAGATGGG TTCTGAGCAG 
AGCCCTTGTC CTGGGTACTC CTCGAGGACT CTTGGCCCAA ATCCTGGACT TATTCTGCAG 
GCTTTGACCC TTTCAAATGC TAGTGATGGA TTTAACCTGG AGCGGCTTGA AATGCTTGGT 
GACTCCTTTT TAAAGCATGC CATCACCACA TATCTATTTT GCACTTATCC TGATGCTCAT 
GAGGGCCGCC TTTCATATAT GAGAAGCAAA AAGGTCAGCA ACTGTAATCT GTATCGGCTT 
GGAAAAAAGA AGGGACTGCC CAGCCGCATG GTGGTATCAA TATTTGATCC CCCTGTGAAT 
TGGCTTCCTC CTGGTTATGT AGTAAACCAA GACAAAAGTA ACGCAGATAA ATGGGAAAAA 
GATGAAATGA CAAAAGACTG CATGTTGGCT AATGGCAAGC TGGACGAGGA CTTTGAGGAG 
GACGACGAGG AGGAGGAGGA TCTAATGTGG AGGGCTCCGA AGGAGGACGC CGATTATGAA 
GATGATTTCC TGGAGTATGA TCAGGAACAT ATCAAATTTA TAGATAACAT GTTAATGGGA 
TCAGGAGCTT TTGTAAAGAA AATTTCTCTC TCTCCTTTTT CAACCACTGA TTCTGCATAC 
GAATGGAAAA TGCCCAAAAA ATCCTCCTTA GGTAGTATGC CATTTTCATC AGATTTTGAG 
GATTTTGACT ATAGCTCTTG GGATGCCATG TGCTATCTGG ATCCTAGCAA AGCTGTGGAA 
GAGGATGACT TTGTGGTGGG GTTCTGGAAT CCATCAGAAG AAAACTGTGG TGTTGACACG 
GGGAAACAGT CCATTTCTTA CGACTTGCAC ACTGAGCAGT GTATTGCGGA CAAAAGCATA 
GCCGACTGCG TGGAAGCCCT GCTGGGCTGC TATTTAACCA GCTGCGGCGA GAGGGCTGCT 
CAGCTCTTCC TCTGCTCACT GGGGCTCAAG GTGCTCCCGG TCATGAAAAG GACCGATCGG 
GAAAAGACCA TGTGCCCTCC TAGGGAGAAT TTCAGCAGCC AACAAAAGAA CCTTTCAGGG 
GGCCGCGCTG CTGCCTCTGT GGCCAGTTTA CGTCCCTCTG TGTTGAAAGA CTTGGAGTAT 
GGCTGTTTGA AGATTCCACC GAGGTGTATG TTTGATCATC CGGACGCAGA TAAAACACTG 
AATCACCTTA TATCGGGTTT TGAGAATTTT GAAAAGAAAA TCAACTACAG ATTCAAGAAT 
AAGGCTTATC TTCTACAGGC TTTTACCCAT GCCTCCTACC ACTACAATAC TATTACTGAT 
TGTTACCAGC GCCTGGAGTT CCTGGGAGAT GCGATCTTGG ACTACCTCAT AACCAAGCAC 
CTTTACGAAG ACCCGCGGCA GCACTCCCCG GGCGTCCTGA CCGACCTGCG GTCCGCACTG 
GTCAACAACA CCATCTTCGC GTCGCTGGCG GTCAAGTACG ACTACCACAA GTACTTCAAA 
GCTGTCTCCC CTGAGCTCTT CCATGTCATT GATGACTTCG TGCAGTTCCA GCTGGAGAAG 
AATGAGATGC AGGGCATGGA CTCCGAGCTT AGGAGGTCTG AGGAGGATGA AGAGAAAGAA 
GAGGACATTG AAGTTCCAAA GGCCATGGGG GACATTTTTG AGTCGCTTGC TGGTGCCATT 
TACATGGATA GTGGGATGTC ACTGGAGATG GTTTGGCAGG TGTACTATCC TATGATGCGG 
CCGCTGATAG AAAAATTTTC TGCAAACGTG CCCCGTTCCC CTGTGCGAGA ATTGCTTGAA 
ATGGAACCAG AAACTGCCAA ATTTAGCCCG GCTGAGAGGA CGTATGACGG CAAGGTCAGA 
GTCACGGTGG AAGTGGTAGG GAAGGGGAAA TTTAAAGGCG TCGGCCGGAG CTACAGGATC 
GCCAAGTCTG CAGCAGCAAG AAGAGCCCTA CGCAGCCTCA AAGCCAATCA ACCTCAGGTT 
CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

DICER1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報