Dicer1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_017314997.1	XP_017170486.1	endoribonuclease Dicer isoform X2
XM_006515609.4	XP_006515672.1	endoribonuclease Dicer isoform X1
XM_017314999.2	XP_017170488.1	endoribonuclease Dicer isoform X4
NM_148948.2	NP_683750.2	endoribonuclease Dicer
XM_006515608.3	XP_006515671.1	endoribonuclease Dicer isoform X1
XM_006515609.3	XP_006515672.1	endoribonuclease Dicer isoform X1
XM_017314998.1	XP_017170487.1	endoribonuclease Dicer isoform X3
XM_006515611.4	XP_006515674.1	endoribonuclease Dicer isoform X1
XM_017314997.2	XP_017170486.1	endoribonuclease Dicer isoform X2
XM_006515611.3	XP_006515674.1	endoribonuclease Dicer isoform X1
XM_017314999.1	XP_017170488.1	endoribonuclease Dicer isoform X4
XM_006515608.4	XP_006515671.1	endoribonuclease Dicer isoform X1
XM_017314998.2	XP_017170487.1	endoribonuclease Dicer isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu78174	XM_017314999.2 Latest version!	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu23015	NM_148948.2 Latest version!	Mus musculus dicer 1, ribonuclease type III (Dicer1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu23015	XM_006515608.4 Latest version!	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu23015	XM_006515611.4 Latest version!	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu23015	XM_006515609.4 Latest version!	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78172	XM_017314997.2 Latest version!	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78173	XM_017314998.2 Latest version!	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMu23015	XM_006515609.3	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu78172	XM_017314997.1	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu23015	XM_006515611.3	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu78174	XM_017314999.1	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu23015	XM_006515608.3	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu78173	XM_017314998.1	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

Dicer1 ( XM_017314999.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170488.1 Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu78174
Clone ID Related Accession (Same CDS sequence)	XM_017314999.2 , XM_017314999.1
Accession Version	XM_017314999.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314999.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGG AGTTCAGAAA GCAGGAGGAG GTACTTAGGA AATTTCGAGC TCATGAGACC 
AACCTGCTCA TTGCAACAAG TGTTGTAGAA GAGGGTGTGG ATATTCCCAA GTGCAATTTG 
GTGGTTCGTT TTGATTTGCC TACAGAGTAT CGATCATATG TCCAGTCTAA AGGACGGGCA 
CGAGCGCCGA TCTCTAATTA CGTAATGTTA GCAGATACAG ACAAGATAAA GAGCTTTGAA 
GAAGACCTCA AGACCTACAA AGCTATTGAA AAGATCTTAA GGAACAAGTG TTCGAAGTCA 
GCGGATGGTG CTGAGGCGGA CGTCCATGCT GGTGTAGATG ATGAGGATGC TTTCCCGCCA 
TATGTGCTGA GGCCTGATGA TGGGGGTCCT CGAGTCACAA TCAACACTGC CATTGGACAC 
ATCAACAGAT ATTGTGCTCG ATTACCAAGT GATCCGTTTA CGCATCTAGC TCCCAAATGT 
AGAACCCGAG AATTGCCTGA TGGTACATTT TATTCAACTC TTTATCTTCC GATTAACTCA 
CCTCTTCGAG CCTCCATTGT TGGTCCACCG ATGGACAGTG TACGGTTGGC TGAAAGAGTG 
GTAGCTCTCA TTTGCTGTGA AAAACTGCAC AAAATTGGTG AACTGGATGA ACACTTGATG 
CCAGTTGGGA AAGAGACTGT TAAATATGAA GAAGAGCTGG ACTTACATGA CGAGGAGGAG 
ACCAGTGTTC CCGGGAGACC AGGGTCCACA AAACGACGGC AGTGCTACCC CAAAGCAATT 
CCAGAGTGCT TGAGGGAGAG TTACCCCAAA CCCGACCAGC CTTGTTACCT GTATGTGATA 
GGAATGGTCT TAACTACCCC CCTCCCTGAT GAACTCAACT TCAGGCGGCG GAAGCTCTAC 
CCACCTGAGG ACACCACAAG ATGCTTTGGG ATCCTGACAG CCAAACCCAT ACCTCAGATT 
CCTCACTTTC CTGTGTATAC ACGCTCTGGA GAGGTCACCA TATCCATCGA GCTGAAGAAG 
TCTGGTTTCA CATTGTCTCA ACAAATGCTC GAGCTGATTA CAAGATTGCA CCAGTACATA 
TTCTCACATA TTCTCCGGCT TGAGAAGCCT GCCCTGGAGT TTAAACCTAC AGGCGCGGAG 
TCAGCCTACT GTGTTCTACC TCTTAATGTT GTTAATGACT CCGGCACTTT GGACATTGAC 
TTTAAATTCA TGGAGGATAT TGAGAAGTCG GAAGCTCGGA TAGGCATTCC TAGCACCAAG 
TATTCAAAGG AAACACCGTT TGTCTTTAAG TTAGAAGATT ACCAAGATGC AGTGATCATT 
CCAAGATACC GCAATTTTGA TCAGCCTCAT CGATTTTATG TAGCTGATGT ATATACGGAT 
CTTACCCCAC TCAGTAAATT TCCTTCCCCT GAATATGAAA CTTTTGCAGA ATATTATAAA 
ACAAAGTATA ACCTTGACCT GACCAATCTC AACCAGCCAC TGCTGGATGT TGACCACACA 
TCTTCCAGAC TGAACCTTTT GACACCTCGG CATTTGAATC AGAAGGGGAA AGCACTTCCT 
CTAAGCAGTG CTGAGAAGAG GAAGGCCAAA TGGGAAAGTC TGCAGAACAA ACAGATCCTG 
GTTCCGGAAC TCTGTGCTAT ACATCCAATC CCAGCATCAC TGTGGAGAAA AGCGGTTTGT 
CTCCCCAGCA TACTGTATCG CCTTCACTGC CTTCTGACTG CAGAGGAACT AAGAGCTCAG 
ACAGCCAGCG ATGCTGGTGT GGGTGTCAGA TCACTTCCCG TGGATTTTAG ATACCCTAAC 
TTAGACTTCG GGTGGAAAAA ATCTATTGAT AGCAAGTCTT TCATCTCAAG CTGTAACTCC 
TCGTTGGCTG AGAGTGATAA TTACTGTAAG CACAGCACAA CTGTAGTCCC TGAACATGCT 
GCACATCAAG GTGCTACTAG ACCCTCCTTA GAAAACCATG ACCAAATGTC TGTGAACTGC 
AAAAGGTTGC CCGCTGAGTC ACCTGCTAAG CTCCAAAGTG AGGTTTCAAC GGATCTTACA 
GCAATTAATG GTCTTTCTTA CAATAAAAAC CTTGCCAATG GCAGTTATGA TTTAGTTAAC 
AGAGACTTTT GCCAAGGAAA TCAGCTGAAT TACTTCAAGC AGGAAATACC TGTACAACCA 
ACTACCTCAT ATCCCATTCA GAATTTGTAC AATTATGAGA ACCAGCCCAA GCCCAGCAAT 
GAATGTCCTC TACTGAGTAA TACGTACCTT GATGGGAACG CTAACACATC TACCTCAGAT 
GGAAGCCCCG CGGTGTCCAC GATGCCTGCC ATGATGAATG CTGTTAAAGC ACTCAAGGAC 
AGGATGGACT CTGAGCAGAG CCCTTCTGTT GGGTACTCCT CAAGGACTCT TGGCCCCAAT 
CCGGGACTGA TTCTCCAGGC TTTGACACTG TCAAATGCCA GTGATGGGTT TAACCTGGAG 
CGGCTTGAGA TGCTTGGCGA CTCCTTTTTA AAGCATGCTA TCACCACATA TCTGTTTTGC 
ACGTACCCTG ATGCTCATGA AGGCCGCCTT TCATATATGA GAAGCAAAAA GGTCAGCAAC 
TGTAACCTGT ATCGCCTTGG CAAGAAGAAG GGACTGCCCA GCCGCATGGT GGTGTCGATA 
TTCGATCCTC CTGTGAATTG GCTTCCTCCT GGTTATGTGG TAAACCAAGA CAAAAGTAAC 
TCAGAGAAGT GGGAAAAGGA TGAAATGACA AAAGACTGCT TGCTGGCTAA TGGCAAACTG 
GGTGAGGCCT GTGAGGAGGA GGAGGACCTG ACGTGGAGGG CCCCCAAGGA GGAGGCCGAG 
GATGAGGACG ACTTCCTGGA GTATGACCAG GAGCATATCC AGTTTATAGA CAGCATGCTC 
ATGGGCTCGG GCGCCTTCGT GAGGAAAATC TCTCTGTCGC CCTTCTCAGC CTCCGACTCT 
GCATACGAAT GGAAGATGCC CAAGAAGGCC TCCTTGGGCA GTATGCCGTT TGCCTCGGGC 
CTTGAGGATT TTGATTACAG CTCTTGGGAT GCGATGTGCT ATCTGGATCC TAGCAAAGCC 
GTTGAAGAGG ATGACTTTGT GGTAGGTTTC TGGAATCCAT CAGAAGAGAA CTGTGGCGTC 
GACACAGGGA AGCAGTCCAT TTCTTACGAC TTGCACACGG AGCAGTGCAT TGCTGACAAG 
AGCATAGCGG ACTGTGTGGA GGCCCTGCTG GGCTGCTACT TAACCAGCTG CGGCGAGAGG 
GCTGCTCAGC TCTTCCTCTG TTCGCTGGGG CTGAAGGTGC TCCCAGTAAT CAAAAGGACC 
AGCCGGGAGA AGGCCTTGGA CCCTGCTCAG GAGAACGGAA GCAGCCAACA AAAGAGCCTT 
TCAGGGAGCT GTGCCTCTCC AGTTGGCCCA CGCTCCTCTG CAGGGAAAGA CTTGGAGTAC 
GGCTGCCTGA AGATCCCACC GAGGTGCATG TTTGACCATC CAGACGCAGA GAAAACCCTC 
AACCACCTGA TATCTGGGTT TGAAACTTTT GAGAAGAAAA TTAACTATAG ATTCAAGAAT 
AAGGCTTATC TTCTGCAGGC TTTTACACAC GCCTCCTACC ACTACAACAC TATCACTGAT 
TGTTACCAGC GCTTAGAATT CCTGGGAGAT GCGATTTTGG ACTACCTCAT AACCAAGCAC 
CTTTATGAAG ACCCACGGCA GCATTCTCCA GGGGTCTTGA CTGACTTGCG CTCTGCCCTT 
GTCAATAACA CCATCTTTGC ATCATTGGCT GTGAAGTATG ACTACCACAA GTACTTTAAA 
GCTGTCTCTC CTGAGCTCTT TCATGTTATT GATGACTTTG TGAAGTTTCA GCTTGAGAAG 
AACGAAATGC AAGGAATGGA CTCTGAGCTT AGGAGATCCG AGGAGGATGA GGAGAAAGAA 
GAGGATATCG AAGTCCCCAA GGCCATGGGG GACATTTTTG AGTCTCTTGC TGGTGCCATT 
TATATGGACA GTGGGATGTC TCTGGAGGTG GTCTGGCAGG TGTACTATCC GATGATGCAG 
CCTCTAATAG AAAAGTTTTC TGCAAATGTG CCTCGTTCTC CAGTGAGAGA ATTGCTCGAG 
ATGGAACCAG AAACTGCCAA GTTTAGCCCA GCGGAGAGAA CTTATGATGG AAAGGTCAGA 
GTCACCGTGG AAGTGGTGGG AAAGGGGAAA TTTAAAGGTG TTGGCCGGAG CTACAGGATC 
GCCAAGTCTG CTGCAGCACG AAGGGCCCTC CGCAGCCTCA AAGCTAACCA GCCTCAGGTT 
CCTAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170488.1
CDS	1027..5298
Translation	MEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_017314999.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314999.2

ORF Insert Sequence:

ATGGAGGCGG AGTTCAGAAA GCAGGAGGAG GTACTTAGGA AATTTCGAGC TCATGAGACC 
AACCTGCTCA TTGCAACAAG TGTTGTAGAA GAGGGTGTGG ATATTCCCAA GTGCAATTTG 
GTGGTTCGTT TTGATTTGCC TACAGAGTAT CGATCATATG TCCAGTCTAA AGGACGGGCA 
CGAGCGCCGA TCTCTAATTA CGTAATGTTA GCAGATACAG ACAAGATAAA GAGCTTTGAA 
GAAGACCTCA AGACCTACAA AGCTATTGAA AAGATCTTAA GGAACAAGTG TTCGAAGTCA 
GCGGATGGTG CTGAGGCGGA CGTCCATGCT GGTGTAGATG ATGAGGATGC TTTCCCGCCA 
TATGTGCTGA GGCCTGATGA TGGGGGTCCT CGAGTCACAA TCAACACTGC CATTGGACAC 
ATCAACAGAT ATTGTGCTCG ATTACCAAGT GATCCGTTTA CGCATCTAGC TCCCAAATGT 
AGAACCCGAG AATTGCCTGA TGGTACATTT TATTCAACTC TTTATCTTCC GATTAACTCA 
CCTCTTCGAG CCTCCATTGT TGGTCCACCG ATGGACAGTG TACGGTTGGC TGAAAGAGTG 
GTAGCTCTCA TTTGCTGTGA AAAACTGCAC AAAATTGGTG AACTGGATGA ACACTTGATG 
CCAGTTGGGA AAGAGACTGT TAAATATGAA GAAGAGCTGG ACTTACATGA CGAGGAGGAG 
ACCAGTGTTC CCGGGAGACC AGGGTCCACA AAACGACGGC AGTGCTACCC CAAAGCAATT 
CCAGAGTGCT TGAGGGAGAG TTACCCCAAA CCCGACCAGC CTTGTTACCT GTATGTGATA 
GGAATGGTCT TAACTACCCC CCTCCCTGAT GAACTCAACT TCAGGCGGCG GAAGCTCTAC 
CCACCTGAGG ACACCACAAG ATGCTTTGGG ATCCTGACAG CCAAACCCAT ACCTCAGATT 
CCTCACTTTC CTGTGTATAC ACGCTCTGGA GAGGTCACCA TATCCATCGA GCTGAAGAAG 
TCTGGTTTCA CATTGTCTCA ACAAATGCTC GAGCTGATTA CAAGATTGCA CCAGTACATA 
TTCTCACATA TTCTCCGGCT TGAGAAGCCT GCCCTGGAGT TTAAACCTAC AGGCGCGGAG 
TCAGCCTACT GTGTTCTACC TCTTAATGTT GTTAATGACT CCGGCACTTT GGACATTGAC 
TTTAAATTCA TGGAGGATAT TGAGAAGTCG GAAGCTCGGA TAGGCATTCC TAGCACCAAG 
TATTCAAAGG AAACACCGTT TGTCTTTAAG TTAGAAGATT ACCAAGATGC AGTGATCATT 
CCAAGATACC GCAATTTTGA TCAGCCTCAT CGATTTTATG TAGCTGATGT ATATACGGAT 
CTTACCCCAC TCAGTAAATT TCCTTCCCCT GAATATGAAA CTTTTGCAGA ATATTATAAA 
ACAAAGTATA ACCTTGACCT GACCAATCTC AACCAGCCAC TGCTGGATGT TGACCACACA 
TCTTCCAGAC TGAACCTTTT GACACCTCGG CATTTGAATC AGAAGGGGAA AGCACTTCCT 
CTAAGCAGTG CTGAGAAGAG GAAGGCCAAA TGGGAAAGTC TGCAGAACAA ACAGATCCTG 
GTTCCGGAAC TCTGTGCTAT ACATCCAATC CCAGCATCAC TGTGGAGAAA AGCGGTTTGT 
CTCCCCAGCA TACTGTATCG CCTTCACTGC CTTCTGACTG CAGAGGAACT AAGAGCTCAG 
ACAGCCAGCG ATGCTGGTGT GGGTGTCAGA TCACTTCCCG TGGATTTTAG ATACCCTAAC 
TTAGACTTCG GGTGGAAAAA ATCTATTGAT AGCAAGTCTT TCATCTCAAG CTGTAACTCC 
TCGTTGGCTG AGAGTGATAA TTACTGTAAG CACAGCACAA CTGTAGTCCC TGAACATGCT 
GCACATCAAG GTGCTACTAG ACCCTCCTTA GAAAACCATG ACCAAATGTC TGTGAACTGC 
AAAAGGTTGC CCGCTGAGTC ACCTGCTAAG CTCCAAAGTG AGGTTTCAAC GGATCTTACA 
GCAATTAATG GTCTTTCTTA CAATAAAAAC CTTGCCAATG GCAGTTATGA TTTAGTTAAC 
AGAGACTTTT GCCAAGGAAA TCAGCTGAAT TACTTCAAGC AGGAAATACC TGTACAACCA 
ACTACCTCAT ATCCCATTCA GAATTTGTAC AATTATGAGA ACCAGCCCAA GCCCAGCAAT 
GAATGTCCTC TACTGAGTAA TACGTACCTT GATGGGAACG CTAACACATC TACCTCAGAT 
GGAAGCCCCG CGGTGTCCAC GATGCCTGCC ATGATGAATG CTGTTAAAGC ACTCAAGGAC 
AGGATGGACT CTGAGCAGAG CCCTTCTGTT GGGTACTCCT CAAGGACTCT TGGCCCCAAT 
CCGGGACTGA TTCTCCAGGC TTTGACACTG TCAAATGCCA GTGATGGGTT TAACCTGGAG 
CGGCTTGAGA TGCTTGGCGA CTCCTTTTTA AAGCATGCTA TCACCACATA TCTGTTTTGC 
ACGTACCCTG ATGCTCATGA AGGCCGCCTT TCATATATGA GAAGCAAAAA GGTCAGCAAC 
TGTAACCTGT ATCGCCTTGG CAAGAAGAAG GGACTGCCCA GCCGCATGGT GGTGTCGATA 
TTCGATCCTC CTGTGAATTG GCTTCCTCCT GGTTATGTGG TAAACCAAGA CAAAAGTAAC 
TCAGAGAAGT GGGAAAAGGA TGAAATGACA AAAGACTGCT TGCTGGCTAA TGGCAAACTG 
GGTGAGGCCT GTGAGGAGGA GGAGGACCTG ACGTGGAGGG CCCCCAAGGA GGAGGCCGAG 
GATGAGGACG ACTTCCTGGA GTATGACCAG GAGCATATCC AGTTTATAGA CAGCATGCTC 
ATGGGCTCGG GCGCCTTCGT GAGGAAAATC TCTCTGTCGC CCTTCTCAGC CTCCGACTCT 
GCATACGAAT GGAAGATGCC CAAGAAGGCC TCCTTGGGCA GTATGCCGTT TGCCTCGGGC 
CTTGAGGATT TTGATTACAG CTCTTGGGAT GCGATGTGCT ATCTGGATCC TAGCAAAGCC 
GTTGAAGAGG ATGACTTTGT GGTAGGTTTC TGGAATCCAT CAGAAGAGAA CTGTGGCGTC 
GACACAGGGA AGCAGTCCAT TTCTTACGAC TTGCACACGG AGCAGTGCAT TGCTGACAAG 
AGCATAGCGG ACTGTGTGGA GGCCCTGCTG GGCTGCTACT TAACCAGCTG CGGCGAGAGG 
GCTGCTCAGC TCTTCCTCTG TTCGCTGGGG CTGAAGGTGC TCCCAGTAAT CAAAAGGACC 
AGCCGGGAGA AGGCCTTGGA CCCTGCTCAG GAGAACGGAA GCAGCCAACA AAAGAGCCTT 
TCAGGGAGCT GTGCCTCTCC AGTTGGCCCA CGCTCCTCTG CAGGGAAAGA CTTGGAGTAC 
GGCTGCCTGA AGATCCCACC GAGGTGCATG TTTGACCATC CAGACGCAGA GAAAACCCTC 
AACCACCTGA TATCTGGGTT TGAAACTTTT GAGAAGAAAA TTAACTATAG ATTCAAGAAT 
AAGGCTTATC TTCTGCAGGC TTTTACACAC GCCTCCTACC ACTACAACAC TATCACTGAT 
TGTTACCAGC GCTTAGAATT CCTGGGAGAT GCGATTTTGG ACTACCTCAT AACCAAGCAC 
CTTTATGAAG ACCCACGGCA GCATTCTCCA GGGGTCTTGA CTGACTTGCG CTCTGCCCTT 
GTCAATAACA CCATCTTTGC ATCATTGGCT GTGAAGTATG ACTACCACAA GTACTTTAAA 
GCTGTCTCTC CTGAGCTCTT TCATGTTATT GATGACTTTG TGAAGTTTCA GCTTGAGAAG 
AACGAAATGC AAGGAATGGA CTCTGAGCTT AGGAGATCCG AGGAGGATGA GGAGAAAGAA 
GAGGATATCG AAGTCCCCAA GGCCATGGGG GACATTTTTG AGTCTCTTGC TGGTGCCATT 
TATATGGACA GTGGGATGTC TCTGGAGGTG GTCTGGCAGG TGTACTATCC GATGATGCAG 
CCTCTAATAG AAAAGTTTTC TGCAAATGTG CCTCGTTCTC CAGTGAGAGA ATTGCTCGAG 
ATGGAACCAG AAACTGCCAA GTTTAGCCCA GCGGAGAGAA CTTATGATGG AAAGGTCAGA 
GTCACCGTGG AAGTGGTGGG AAAGGGGAAA TTTAAAGGTG TTGGCCGGAG CTACAGGATC 
GCCAAGTCTG CTGCAGCACG AAGGGCCCTC CGCAGCCTCA AAGCTAACCA GCCTCAGGTT 
CCTAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dicer1 ( XM_017314999.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170488.1 Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu78174
Clone ID Related Accession (Same CDS sequence)	XM_017314999.2 , XM_017314999.1
Accession Version	XM_017314999.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGG AGTTCAGAAA GCAGGAGGAG GTACTTAGGA AATTTCGAGC TCATGAGACC 
AACCTGCTCA TTGCAACAAG TGTTGTAGAA GAGGGTGTGG ATATTCCCAA GTGCAATTTG 
GTGGTTCGTT TTGATTTGCC TACAGAGTAT CGATCATATG TCCAGTCTAA AGGACGGGCA 
CGAGCGCCGA TCTCTAATTA CGTAATGTTA GCAGATACAG ACAAGATAAA GAGCTTTGAA 
GAAGACCTCA AGACCTACAA AGCTATTGAA AAGATCTTAA GGAACAAGTG TTCGAAGTCA 
GCGGATGGTG CTGAGGCGGA CGTCCATGCT GGTGTAGATG ATGAGGATGC TTTCCCGCCA 
TATGTGCTGA GGCCTGATGA TGGGGGTCCT CGAGTCACAA TCAACACTGC CATTGGACAC 
ATCAACAGAT ATTGTGCTCG ATTACCAAGT GATCCGTTTA CGCATCTAGC TCCCAAATGT 
AGAACCCGAG AATTGCCTGA TGGTACATTT TATTCAACTC TTTATCTTCC GATTAACTCA 
CCTCTTCGAG CCTCCATTGT TGGTCCACCG ATGGACAGTG TACGGTTGGC TGAAAGAGTG 
GTAGCTCTCA TTTGCTGTGA AAAACTGCAC AAAATTGGTG AACTGGATGA ACACTTGATG 
CCAGTTGGGA AAGAGACTGT TAAATATGAA GAAGAGCTGG ACTTACATGA CGAGGAGGAG 
ACCAGTGTTC CCGGGAGACC AGGGTCCACA AAACGACGGC AGTGCTACCC CAAAGCAATT 
CCAGAGTGCT TGAGGGAGAG TTACCCCAAA CCCGACCAGC CTTGTTACCT GTATGTGATA 
GGAATGGTCT TAACTACCCC CCTCCCTGAT GAACTCAACT TCAGGCGGCG GAAGCTCTAC 
CCACCTGAGG ACACCACAAG ATGCTTTGGG ATCCTGACAG CCAAACCCAT ACCTCAGATT 
CCTCACTTTC CTGTGTATAC ACGCTCTGGA GAGGTCACCA TATCCATCGA GCTGAAGAAG 
TCTGGTTTCA CATTGTCTCA ACAAATGCTC GAGCTGATTA CAAGATTGCA CCAGTACATA 
TTCTCACATA TTCTCCGGCT TGAGAAGCCT GCCCTGGAGT TTAAACCTAC AGGCGCGGAG 
TCAGCCTACT GTGTTCTACC TCTTAATGTT GTTAATGACT CCGGCACTTT GGACATTGAC 
TTTAAATTCA TGGAGGATAT TGAGAAGTCG GAAGCTCGGA TAGGCATTCC TAGCACCAAG 
TATTCAAAGG AAACACCGTT TGTCTTTAAG TTAGAAGATT ACCAAGATGC AGTGATCATT 
CCAAGATACC GCAATTTTGA TCAGCCTCAT CGATTTTATG TAGCTGATGT ATATACGGAT 
CTTACCCCAC TCAGTAAATT TCCTTCCCCT GAATATGAAA CTTTTGCAGA ATATTATAAA 
ACAAAGTATA ACCTTGACCT GACCAATCTC AACCAGCCAC TGCTGGATGT TGACCACACA 
TCTTCCAGAC TGAACCTTTT GACACCTCGG CATTTGAATC AGAAGGGGAA AGCACTTCCT 
CTAAGCAGTG CTGAGAAGAG GAAGGCCAAA TGGGAAAGTC TGCAGAACAA ACAGATCCTG 
GTTCCGGAAC TCTGTGCTAT ACATCCAATC CCAGCATCAC TGTGGAGAAA AGCGGTTTGT 
CTCCCCAGCA TACTGTATCG CCTTCACTGC CTTCTGACTG CAGAGGAACT AAGAGCTCAG 
ACAGCCAGCG ATGCTGGTGT GGGTGTCAGA TCACTTCCCG TGGATTTTAG ATACCCTAAC 
TTAGACTTCG GGTGGAAAAA ATCTATTGAT AGCAAGTCTT TCATCTCAAG CTGTAACTCC 
TCGTTGGCTG AGAGTGATAA TTACTGTAAG CACAGCACAA CTGTAGTCCC TGAACATGCT 
GCACATCAAG GTGCTACTAG ACCCTCCTTA GAAAACCATG ACCAAATGTC TGTGAACTGC 
AAAAGGTTGC CCGCTGAGTC ACCTGCTAAG CTCCAAAGTG AGGTTTCAAC GGATCTTACA 
GCAATTAATG GTCTTTCTTA CAATAAAAAC CTTGCCAATG GCAGTTATGA TTTAGTTAAC 
AGAGACTTTT GCCAAGGAAA TCAGCTGAAT TACTTCAAGC AGGAAATACC TGTACAACCA 
ACTACCTCAT ATCCCATTCA GAATTTGTAC AATTATGAGA ACCAGCCCAA GCCCAGCAAT 
GAATGTCCTC TACTGAGTAA TACGTACCTT GATGGGAACG CTAACACATC TACCTCAGAT 
GGAAGCCCCG CGGTGTCCAC GATGCCTGCC ATGATGAATG CTGTTAAAGC ACTCAAGGAC 
AGGATGGACT CTGAGCAGAG CCCTTCTGTT GGGTACTCCT CAAGGACTCT TGGCCCCAAT 
CCGGGACTGA TTCTCCAGGC TTTGACACTG TCAAATGCCA GTGATGGGTT TAACCTGGAG 
CGGCTTGAGA TGCTTGGCGA CTCCTTTTTA AAGCATGCTA TCACCACATA TCTGTTTTGC 
ACGTACCCTG ATGCTCATGA AGGCCGCCTT TCATATATGA GAAGCAAAAA GGTCAGCAAC 
TGTAACCTGT ATCGCCTTGG CAAGAAGAAG GGACTGCCCA GCCGCATGGT GGTGTCGATA 
TTCGATCCTC CTGTGAATTG GCTTCCTCCT GGTTATGTGG TAAACCAAGA CAAAAGTAAC 
TCAGAGAAGT GGGAAAAGGA TGAAATGACA AAAGACTGCT TGCTGGCTAA TGGCAAACTG 
GGTGAGGCCT GTGAGGAGGA GGAGGACCTG ACGTGGAGGG CCCCCAAGGA GGAGGCCGAG 
GATGAGGACG ACTTCCTGGA GTATGACCAG GAGCATATCC AGTTTATAGA CAGCATGCTC 
ATGGGCTCGG GCGCCTTCGT GAGGAAAATC TCTCTGTCGC CCTTCTCAGC CTCCGACTCT 
GCATACGAAT GGAAGATGCC CAAGAAGGCC TCCTTGGGCA GTATGCCGTT TGCCTCGGGC 
CTTGAGGATT TTGATTACAG CTCTTGGGAT GCGATGTGCT ATCTGGATCC TAGCAAAGCC 
GTTGAAGAGG ATGACTTTGT GGTAGGTTTC TGGAATCCAT CAGAAGAGAA CTGTGGCGTC 
GACACAGGGA AGCAGTCCAT TTCTTACGAC TTGCACACGG AGCAGTGCAT TGCTGACAAG 
AGCATAGCGG ACTGTGTGGA GGCCCTGCTG GGCTGCTACT TAACCAGCTG CGGCGAGAGG 
GCTGCTCAGC TCTTCCTCTG TTCGCTGGGG CTGAAGGTGC TCCCAGTAAT CAAAAGGACC 
AGCCGGGAGA AGGCCTTGGA CCCTGCTCAG GAGAACGGAA GCAGCCAACA AAAGAGCCTT 
TCAGGGAGCT GTGCCTCTCC AGTTGGCCCA CGCTCCTCTG CAGGGAAAGA CTTGGAGTAC 
GGCTGCCTGA AGATCCCACC GAGGTGCATG TTTGACCATC CAGACGCAGA GAAAACCCTC 
AACCACCTGA TATCTGGGTT TGAAACTTTT GAGAAGAAAA TTAACTATAG ATTCAAGAAT 
AAGGCTTATC TTCTGCAGGC TTTTACACAC GCCTCCTACC ACTACAACAC TATCACTGAT 
TGTTACCAGC GCTTAGAATT CCTGGGAGAT GCGATTTTGG ACTACCTCAT AACCAAGCAC 
CTTTATGAAG ACCCACGGCA GCATTCTCCA GGGGTCTTGA CTGACTTGCG CTCTGCCCTT 
GTCAATAACA CCATCTTTGC ATCATTGGCT GTGAAGTATG ACTACCACAA GTACTTTAAA 
GCTGTCTCTC CTGAGCTCTT TCATGTTATT GATGACTTTG TGAAGTTTCA GCTTGAGAAG 
AACGAAATGC AAGGAATGGA CTCTGAGCTT AGGAGATCCG AGGAGGATGA GGAGAAAGAA 
GAGGATATCG AAGTCCCCAA GGCCATGGGG GACATTTTTG AGTCTCTTGC TGGTGCCATT 
TATATGGACA GTGGGATGTC TCTGGAGGTG GTCTGGCAGG TGTACTATCC GATGATGCAG 
CCTCTAATAG AAAAGTTTTC TGCAAATGTG CCTCGTTCTC CAGTGAGAGA ATTGCTCGAG 
ATGGAACCAG AAACTGCCAA GTTTAGCCCA GCGGAGAGAA CTTATGATGG AAAGGTCAGA 
GTCACCGTGG AAGTGGTGGG AAAGGGGAAA TTTAAAGGTG TTGGCCGGAG CTACAGGATC 
GCCAAGTCTG CTGCAGCACG AAGGGCCCTC CGCAGCCTCA AAGCTAACCA GCCTCAGGTT 
CCTAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170488.1
CDS	1020..5291
Translation	MEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_017314999.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314999.1

ORF Insert Sequence:

ATGGAGGCGG AGTTCAGAAA GCAGGAGGAG GTACTTAGGA AATTTCGAGC TCATGAGACC 
AACCTGCTCA TTGCAACAAG TGTTGTAGAA GAGGGTGTGG ATATTCCCAA GTGCAATTTG 
GTGGTTCGTT TTGATTTGCC TACAGAGTAT CGATCATATG TCCAGTCTAA AGGACGGGCA 
CGAGCGCCGA TCTCTAATTA CGTAATGTTA GCAGATACAG ACAAGATAAA GAGCTTTGAA 
GAAGACCTCA AGACCTACAA AGCTATTGAA AAGATCTTAA GGAACAAGTG TTCGAAGTCA 
GCGGATGGTG CTGAGGCGGA CGTCCATGCT GGTGTAGATG ATGAGGATGC TTTCCCGCCA 
TATGTGCTGA GGCCTGATGA TGGGGGTCCT CGAGTCACAA TCAACACTGC CATTGGACAC 
ATCAACAGAT ATTGTGCTCG ATTACCAAGT GATCCGTTTA CGCATCTAGC TCCCAAATGT 
AGAACCCGAG AATTGCCTGA TGGTACATTT TATTCAACTC TTTATCTTCC GATTAACTCA 
CCTCTTCGAG CCTCCATTGT TGGTCCACCG ATGGACAGTG TACGGTTGGC TGAAAGAGTG 
GTAGCTCTCA TTTGCTGTGA AAAACTGCAC AAAATTGGTG AACTGGATGA ACACTTGATG 
CCAGTTGGGA AAGAGACTGT TAAATATGAA GAAGAGCTGG ACTTACATGA CGAGGAGGAG 
ACCAGTGTTC CCGGGAGACC AGGGTCCACA AAACGACGGC AGTGCTACCC CAAAGCAATT 
CCAGAGTGCT TGAGGGAGAG TTACCCCAAA CCCGACCAGC CTTGTTACCT GTATGTGATA 
GGAATGGTCT TAACTACCCC CCTCCCTGAT GAACTCAACT TCAGGCGGCG GAAGCTCTAC 
CCACCTGAGG ACACCACAAG ATGCTTTGGG ATCCTGACAG CCAAACCCAT ACCTCAGATT 
CCTCACTTTC CTGTGTATAC ACGCTCTGGA GAGGTCACCA TATCCATCGA GCTGAAGAAG 
TCTGGTTTCA CATTGTCTCA ACAAATGCTC GAGCTGATTA CAAGATTGCA CCAGTACATA 
TTCTCACATA TTCTCCGGCT TGAGAAGCCT GCCCTGGAGT TTAAACCTAC AGGCGCGGAG 
TCAGCCTACT GTGTTCTACC TCTTAATGTT GTTAATGACT CCGGCACTTT GGACATTGAC 
TTTAAATTCA TGGAGGATAT TGAGAAGTCG GAAGCTCGGA TAGGCATTCC TAGCACCAAG 
TATTCAAAGG AAACACCGTT TGTCTTTAAG TTAGAAGATT ACCAAGATGC AGTGATCATT 
CCAAGATACC GCAATTTTGA TCAGCCTCAT CGATTTTATG TAGCTGATGT ATATACGGAT 
CTTACCCCAC TCAGTAAATT TCCTTCCCCT GAATATGAAA CTTTTGCAGA ATATTATAAA 
ACAAAGTATA ACCTTGACCT GACCAATCTC AACCAGCCAC TGCTGGATGT TGACCACACA 
TCTTCCAGAC TGAACCTTTT GACACCTCGG CATTTGAATC AGAAGGGGAA AGCACTTCCT 
CTAAGCAGTG CTGAGAAGAG GAAGGCCAAA TGGGAAAGTC TGCAGAACAA ACAGATCCTG 
GTTCCGGAAC TCTGTGCTAT ACATCCAATC CCAGCATCAC TGTGGAGAAA AGCGGTTTGT 
CTCCCCAGCA TACTGTATCG CCTTCACTGC CTTCTGACTG CAGAGGAACT AAGAGCTCAG 
ACAGCCAGCG ATGCTGGTGT GGGTGTCAGA TCACTTCCCG TGGATTTTAG ATACCCTAAC 
TTAGACTTCG GGTGGAAAAA ATCTATTGAT AGCAAGTCTT TCATCTCAAG CTGTAACTCC 
TCGTTGGCTG AGAGTGATAA TTACTGTAAG CACAGCACAA CTGTAGTCCC TGAACATGCT 
GCACATCAAG GTGCTACTAG ACCCTCCTTA GAAAACCATG ACCAAATGTC TGTGAACTGC 
AAAAGGTTGC CCGCTGAGTC ACCTGCTAAG CTCCAAAGTG AGGTTTCAAC GGATCTTACA 
GCAATTAATG GTCTTTCTTA CAATAAAAAC CTTGCCAATG GCAGTTATGA TTTAGTTAAC 
AGAGACTTTT GCCAAGGAAA TCAGCTGAAT TACTTCAAGC AGGAAATACC TGTACAACCA 
ACTACCTCAT ATCCCATTCA GAATTTGTAC AATTATGAGA ACCAGCCCAA GCCCAGCAAT 
GAATGTCCTC TACTGAGTAA TACGTACCTT GATGGGAACG CTAACACATC TACCTCAGAT 
GGAAGCCCCG CGGTGTCCAC GATGCCTGCC ATGATGAATG CTGTTAAAGC ACTCAAGGAC 
AGGATGGACT CTGAGCAGAG CCCTTCTGTT GGGTACTCCT CAAGGACTCT TGGCCCCAAT 
CCGGGACTGA TTCTCCAGGC TTTGACACTG TCAAATGCCA GTGATGGGTT TAACCTGGAG 
CGGCTTGAGA TGCTTGGCGA CTCCTTTTTA AAGCATGCTA TCACCACATA TCTGTTTTGC 
ACGTACCCTG ATGCTCATGA AGGCCGCCTT TCATATATGA GAAGCAAAAA GGTCAGCAAC 
TGTAACCTGT ATCGCCTTGG CAAGAAGAAG GGACTGCCCA GCCGCATGGT GGTGTCGATA 
TTCGATCCTC CTGTGAATTG GCTTCCTCCT GGTTATGTGG TAAACCAAGA CAAAAGTAAC 
TCAGAGAAGT GGGAAAAGGA TGAAATGACA AAAGACTGCT TGCTGGCTAA TGGCAAACTG 
GGTGAGGCCT GTGAGGAGGA GGAGGACCTG ACGTGGAGGG CCCCCAAGGA GGAGGCCGAG 
GATGAGGACG ACTTCCTGGA GTATGACCAG GAGCATATCC AGTTTATAGA CAGCATGCTC 
ATGGGCTCGG GCGCCTTCGT GAGGAAAATC TCTCTGTCGC CCTTCTCAGC CTCCGACTCT 
GCATACGAAT GGAAGATGCC CAAGAAGGCC TCCTTGGGCA GTATGCCGTT TGCCTCGGGC 
CTTGAGGATT TTGATTACAG CTCTTGGGAT GCGATGTGCT ATCTGGATCC TAGCAAAGCC 
GTTGAAGAGG ATGACTTTGT GGTAGGTTTC TGGAATCCAT CAGAAGAGAA CTGTGGCGTC 
GACACAGGGA AGCAGTCCAT TTCTTACGAC TTGCACACGG AGCAGTGCAT TGCTGACAAG 
AGCATAGCGG ACTGTGTGGA GGCCCTGCTG GGCTGCTACT TAACCAGCTG CGGCGAGAGG 
GCTGCTCAGC TCTTCCTCTG TTCGCTGGGG CTGAAGGTGC TCCCAGTAAT CAAAAGGACC 
AGCCGGGAGA AGGCCTTGGA CCCTGCTCAG GAGAACGGAA GCAGCCAACA AAAGAGCCTT 
TCAGGGAGCT GTGCCTCTCC AGTTGGCCCA CGCTCCTCTG CAGGGAAAGA CTTGGAGTAC 
GGCTGCCTGA AGATCCCACC GAGGTGCATG TTTGACCATC CAGACGCAGA GAAAACCCTC 
AACCACCTGA TATCTGGGTT TGAAACTTTT GAGAAGAAAA TTAACTATAG ATTCAAGAAT 
AAGGCTTATC TTCTGCAGGC TTTTACACAC GCCTCCTACC ACTACAACAC TATCACTGAT 
TGTTACCAGC GCTTAGAATT CCTGGGAGAT GCGATTTTGG ACTACCTCAT AACCAAGCAC 
CTTTATGAAG ACCCACGGCA GCATTCTCCA GGGGTCTTGA CTGACTTGCG CTCTGCCCTT 
GTCAATAACA CCATCTTTGC ATCATTGGCT GTGAAGTATG ACTACCACAA GTACTTTAAA 
GCTGTCTCTC CTGAGCTCTT TCATGTTATT GATGACTTTG TGAAGTTTCA GCTTGAGAAG 
AACGAAATGC AAGGAATGGA CTCTGAGCTT AGGAGATCCG AGGAGGATGA GGAGAAAGAA 
GAGGATATCG AAGTCCCCAA GGCCATGGGG GACATTTTTG AGTCTCTTGC TGGTGCCATT 
TATATGGACA GTGGGATGTC TCTGGAGGTG GTCTGGCAGG TGTACTATCC GATGATGCAG 
CCTCTAATAG AAAAGTTTTC TGCAAATGTG CCTCGTTCTC CAGTGAGAGA ATTGCTCGAG 
ATGGAACCAG AAACTGCCAA GTTTAGCCCA GCGGAGAGAA CTTATGATGG AAAGGTCAGA 
GTCACCGTGG AAGTGGTGGG AAAGGGGAAA TTTAAAGGTG TTGGCCGGAG CTACAGGATC 
GCCAAGTCTG CTGCAGCACG AAGGGCCCTC CGCAGCCTCA AAGCTAACCA GCCTCAGGTT 
CCTAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dicer1 ( NM_148948.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_683750.2 Mus musculus dicer 1, ribonuclease type III (Dicer1), mRNA.

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CloneID	OMu23015
Clone ID Related Accession (Same CDS sequence)	XM_006515609.4 , NM_148948.2 , XM_006515608.3 , XM_006515609.3 , XM_006515611.4 , XM_006515611.3 , XM_006515608.4
Accession Version	NM_148948.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124364.4. On Nov 1, 2006 this sequence version replaced NM_148948.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF430845.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683750.2
CDS	280..6000
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSDLEVNASWTKERWSQEFTKHQVLIMTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	NM_148948.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148948.2

ORF Insert Sequence:

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dicer1 ( XM_006515609.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515672.1 Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X2, mRNA.

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CloneID	OMu23015
Clone ID Related Accession (Same CDS sequence)	XM_006515609.4 , NM_148948.2 , XM_006515608.3 , XM_006515609.3 , XM_006515611.4 , XM_006515611.3 , XM_006515608.4
Accession Version	XM_006515609.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515609.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515672.1
CDS	327..6047
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSDLEVNASWTKERWSQEFTKHQVLIMTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_006515609.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515609.4

ORF Insert Sequence:

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dicer1 ( XM_006515608.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515671.1 Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X1, mRNA.

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CloneID	OMu23015
Clone ID Related Accession (Same CDS sequence)	XM_006515609.4 , NM_148948.2 , XM_006515608.3 , XM_006515609.3 , XM_006515611.4 , XM_006515611.3 , XM_006515608.4
Accession Version	XM_006515608.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515608.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515671.1
CDS	816..6536
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSDLEVNASWTKERWSQEFTKHQVLIMTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_006515608.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515608.3

ORF Insert Sequence:

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dicer1 ( XM_006515609.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515672.1 Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X2, mRNA.

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CloneID	OMu23015
Clone ID Related Accession (Same CDS sequence)	XM_006515609.4 , NM_148948.2 , XM_006515608.3 , XM_006515609.3 , XM_006515611.4 , XM_006515611.3 , XM_006515608.4
Accession Version	XM_006515609.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515609.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515672.1
CDS	1138..6858
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSDLEVNASWTKERWSQEFTKHQVLIMTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_006515609.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515609.3

ORF Insert Sequence:

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dicer1 ( XM_006515611.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515674.1 Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X3, mRNA.

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CloneID	OMu23015
Clone ID Related Accession (Same CDS sequence)	XM_006515609.4 , NM_148948.2 , XM_006515608.3 , XM_006515609.3 , XM_006515611.4 , XM_006515611.3 , XM_006515608.4
Accession Version	XM_006515611.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515611.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515674.1
CDS	362..6082
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSDLEVNASWTKERWSQEFTKHQVLIMTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_006515611.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515611.4

ORF Insert Sequence:

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dicer1 ( XM_006515611.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515674.1 Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X3, mRNA.

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CloneID	OMu23015
Clone ID Related Accession (Same CDS sequence)	XM_006515609.4 , NM_148948.2 , XM_006515608.3 , XM_006515609.3 , XM_006515611.4 , XM_006515611.3 , XM_006515608.4
Accession Version	XM_006515611.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515674.1
CDS	718..6438
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSDLEVNASWTKERWSQEFTKHQVLIMTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_006515611.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515611.3

ORF Insert Sequence:

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dicer1 ( XM_006515608.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515671.1 Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X1, mRNA.

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CloneID	OMu23015
Clone ID Related Accession (Same CDS sequence)	XM_006515609.4 , NM_148948.2 , XM_006515608.3 , XM_006515609.3 , XM_006515611.4 , XM_006515611.3 , XM_006515608.4
Accession Version	XM_006515608.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515608.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515671.1
CDS	440..6160
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSDLEVNASWTKERWSQEFTKHQVLIMTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_006515608.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515608.4

ORF Insert Sequence:

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGCTTA GGAGATCCGA GGAGGATGAG 
GAGAAAGAAG AGGATATCGA AGTCCCCAAG GCCATGGGGG ACATTTTTGA GTCTCTTGCT 
GGTGCCATTT ATATGGACAG TGGGATGTCT CTGGAGGTGG TCTGGCAGGT GTACTATCCG 
ATGATGCAGC CTCTAATAGA AAAGTTTTCT GCAAATGTGC CTCGTTCTCC AGTGAGAGAA 
TTGCTCGAGA TGGAACCAGA AACTGCCAAG TTTAGCCCAG CGGAGAGAAC TTATGATGGA 
AAGGTCAGAG TCACCGTGGA AGTGGTGGGA AAGGGGAAAT TTAAAGGTGT TGGCCGGAGC 
TACAGGATCG CCAAGTCTGC TGCAGCACGA AGGGCCCTCC GCAGCCTCAA AGCTAACCAG 
CCTCAGGTTC CTAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dicer1 ( XM_017314997.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170486.1 Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X4, mRNA.

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CloneID	OMu78172
Clone ID Related Accession (Same CDS sequence)	XM_017314997.1 , XM_017314997.2
Accession Version	XM_017314997.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGAAAA GTTTTCTGCA AATGTGCCTC 
GTTCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170486.1
CDS	816..6167
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSDLEVNASWTKERWSQEFTKHQVLIMTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSEKSFLQMCLVLQ

Target ORF information:

RefSeq Version	XM_017314997.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314997.1

ORF Insert Sequence:

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGAAAA GTTTTCTGCA AATGTGCCTC 
GTTCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dicer1 ( XM_017314997.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170486.1 Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X4, mRNA.

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CloneID	OMu78172
Clone ID Related Accession (Same CDS sequence)	XM_017314997.1 , XM_017314997.2
Accession Version	XM_017314997.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGAAAA GTTTTCTGCA AATGTGCCTC 
GTTCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170486.1
CDS	440..5791
Translation	MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSDLEVNASWTKERWSQEFTKHQVLIMTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSEKSFLQMCLVLQ

Target ORF information:

RefSeq Version	XM_017314997.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314997.2

ORF Insert Sequence:

ATGGCAGGCC TGCAGCTCAT GACCCCTGCT TCCTCACCAA TGGGTCCTTT CTTTGGACTG 
CCATGGCAAC AAGAAGCAAT TCATGATAAC ATTTATACGC CAAGAAAATA CCAGGTTGAG 
CTGCTGGAAG CAGCTCTGGA CCATAACACA ATTGTGTGCT TGAACACTGG CTCAGGGAAG 
ACGTTCATCG CGGTCCTGCT CACCAAAGAG CTGGCCCATC AGATCAGGGG CGACCTCAAC 
CCGCATGCAA AAAGGACCGT GTTCCTCGTC AACTCTGCAA ACCAGGTTGC TCAACAAGTG 
TCAGCCGTCA GAACTCACTC GGATCTCAAG GTTGGAGAGT ACTCAGACCT AGAAGTAAAT 
GCATCTTGGA CAAAAGAGAG ATGGAGCCAA GAGTTTACTA AGCACCAGGT TCTCATTATG 
ACTTGCTATG TCGCCTTGAC TGTTTTGAAA AATGGTTACT TATCACTGTC AGACATTAAC 
CTTTTGGTGT TTGATGAGTG TCATCTTGCG ATTCTAGACC ACCCCTATCG TGAAATTATG 
AAGCTCTGTG AGAGTTGTCC ATCATGCCCT CGAATTTTGG GACTAACTGC TTCCATTTTA 
AATGGGAAAT GTGATCCAGA GGAATTGGAA GAGAAGATTC AGAAACTGGA GCGAATTCTC 
AGGAGTGACG CTGAGACGGC GACTGACTTG GTGGTCCTGG ACAGATACAC TTCTCAGCCT 
TGTGAGATTG TGGTAGACTG TGGACCGTTT ACTGACAGAA GTGGGCTGTA TGAGAGATTG 
CTGATGGAGT TAGAAGCAGC ACTTGATTTT ATCAATGATT GTAATGTAGC TGTACATTCA 
AAGGAAAGAG ATTCTACTTT GATTTCCAAA CAAATCCTCT CAGACTGCCG TGCAGTGTTG 
GTTGTTCTGG GACCCTGGTG TGCAGATAAA GTAGCTGGGA TGATGGTAAG AGAACTTCAG 
AAGTACATCA AACATGAGCA AGAGGAGCTA CACAGGAAGT TCCTATTGTT TACAGACACC 
TTGTTAAGGA AAATACACGC CCTGTGTGAA GAGTACTTCT CACCTGCCTC ACTTGACCTG 
AAGTATGTAA CTCCCAAAGT CATGAAACTG CTCGAGATCC TGCGCAAATA CAAGCCCTAT 
GAGCGACAGC AGTTTGAAAG TGTTGAGTGG TACAATAACA GGAATCAGGA TAATTACGTG 
TCCTGGAGTG ATTCCGAGGA TGACGATGAT GACGAAGAAA TTGAAGAAAA AGAAAAGCCA 
GAGACAAATT TTCCTTCTCC GTTTACCAAT ATTTTATGTG GGATTATTTT TGTGGAAAGA 
AGATACACAG CAGTTGTCCT AAACAGATTG ATAAAGGAAG CTGGCAAACA AGATCCAGAG 
CTGGCTTATA TCAGCAGCAA CTTTATAACT GGACACGGCA TTGGAAAGAA CCAGCCTCGC 
AGCAAACAGA TGGAGGCGGA GTTCAGAAAG CAGGAGGAGG TACTTAGGAA ATTTCGAGCT 
CATGAGACCA ACCTGCTCAT TGCAACAAGT GTTGTAGAAG AGGGTGTGGA TATTCCCAAG 
TGCAATTTGG TGGTTCGTTT TGATTTGCCT ACAGAGTATC GATCATATGT CCAGTCTAAA 
GGACGGGCAC GAGCGCCGAT CTCTAATTAC GTAATGTTAG CAGATACAGA CAAGATAAAG 
AGCTTTGAAG AAGACCTCAA GACCTACAAA GCTATTGAAA AGATCTTAAG GAACAAGTGT 
TCGAAGTCAG CGGATGGTGC TGAGGCGGAC GTCCATGCTG GTGTAGATGA TGAGGATGCT 
TTCCCGCCAT ATGTGCTGAG GCCTGATGAT GGGGGTCCTC GAGTCACAAT CAACACTGCC 
ATTGGACACA TCAACAGATA TTGTGCTCGA TTACCAAGTG ATCCGTTTAC GCATCTAGCT 
CCCAAATGTA GAACCCGAGA ATTGCCTGAT GGTACATTTT ATTCAACTCT TTATCTTCCG 
ATTAACTCAC CTCTTCGAGC CTCCATTGTT GGTCCACCGA TGGACAGTGT ACGGTTGGCT 
GAAAGAGTGG TAGCTCTCAT TTGCTGTGAA AAACTGCACA AAATTGGTGA ACTGGATGAA 
CACTTGATGC CAGTTGGGAA AGAGACTGTT AAATATGAAG AAGAGCTGGA CTTACATGAC 
GAGGAGGAGA CCAGTGTTCC CGGGAGACCA GGGTCCACAA AACGACGGCA GTGCTACCCC 
AAAGCAATTC CAGAGTGCTT GAGGGAGAGT TACCCCAAAC CCGACCAGCC TTGTTACCTG 
TATGTGATAG GAATGGTCTT AACTACCCCC CTCCCTGATG AACTCAACTT CAGGCGGCGG 
AAGCTCTACC CACCTGAGGA CACCACAAGA TGCTTTGGGA TCCTGACAGC CAAACCCATA 
CCTCAGATTC CTCACTTTCC TGTGTATACA CGCTCTGGAG AGGTCACCAT ATCCATCGAG 
CTGAAGAAGT CTGGTTTCAC ATTGTCTCAA CAAATGCTCG AGCTGATTAC AAGATTGCAC 
CAGTACATAT TCTCACATAT TCTCCGGCTT GAGAAGCCTG CCCTGGAGTT TAAACCTACA 
GGCGCGGAGT CAGCCTACTG TGTTCTACCT CTTAATGTTG TTAATGACTC CGGCACTTTG 
GACATTGACT TTAAATTCAT GGAGGATATT GAGAAGTCGG AAGCTCGGAT AGGCATTCCT 
AGCACCAAGT ATTCAAAGGA AACACCGTTT GTCTTTAAGT TAGAAGATTA CCAAGATGCA 
GTGATCATTC CAAGATACCG CAATTTTGAT CAGCCTCATC GATTTTATGT AGCTGATGTA 
TATACGGATC TTACCCCACT CAGTAAATTT CCTTCCCCTG AATATGAAAC TTTTGCAGAA 
TATTATAAAA CAAAGTATAA CCTTGACCTG ACCAATCTCA ACCAGCCACT GCTGGATGTT 
GACCACACAT CTTCCAGACT GAACCTTTTG ACACCTCGGC ATTTGAATCA GAAGGGGAAA 
GCACTTCCTC TAAGCAGTGC TGAGAAGAGG AAGGCCAAAT GGGAAAGTCT GCAGAACAAA 
CAGATCCTGG TTCCGGAACT CTGTGCTATA CATCCAATCC CAGCATCACT GTGGAGAAAA 
GCGGTTTGTC TCCCCAGCAT ACTGTATCGC CTTCACTGCC TTCTGACTGC AGAGGAACTA 
AGAGCTCAGA CAGCCAGCGA TGCTGGTGTG GGTGTCAGAT CACTTCCCGT GGATTTTAGA 
TACCCTAACT TAGACTTCGG GTGGAAAAAA TCTATTGATA GCAAGTCTTT CATCTCAAGC 
TGTAACTCCT CGTTGGCTGA GAGTGATAAT TACTGTAAGC ACAGCACAAC TGTAGTCCCT 
GAACATGCTG CACATCAAGG TGCTACTAGA CCCTCCTTAG AAAACCATGA CCAAATGTCT 
GTGAACTGCA AAAGGTTGCC CGCTGAGTCA CCTGCTAAGC TCCAAAGTGA GGTTTCAACG 
GATCTTACAG CAATTAATGG TCTTTCTTAC AATAAAAACC TTGCCAATGG CAGTTATGAT 
TTAGTTAACA GAGACTTTTG CCAAGGAAAT CAGCTGAATT ACTTCAAGCA GGAAATACCT 
GTACAACCAA CTACCTCATA TCCCATTCAG AATTTGTACA ATTATGAGAA CCAGCCCAAG 
CCCAGCAATG AATGTCCTCT ACTGAGTAAT ACGTACCTTG ATGGGAACGC TAACACATCT 
ACCTCAGATG GAAGCCCCGC GGTGTCCACG ATGCCTGCCA TGATGAATGC TGTTAAAGCA 
CTCAAGGACA GGATGGACTC TGAGCAGAGC CCTTCTGTTG GGTACTCCTC AAGGACTCTT 
GGCCCCAATC CGGGACTGAT TCTCCAGGCT TTGACACTGT CAAATGCCAG TGATGGGTTT 
AACCTGGAGC GGCTTGAGAT GCTTGGCGAC TCCTTTTTAA AGCATGCTAT CACCACATAT 
CTGTTTTGCA CGTACCCTGA TGCTCATGAA GGCCGCCTTT CATATATGAG AAGCAAAAAG 
GTCAGCAACT GTAACCTGTA TCGCCTTGGC AAGAAGAAGG GACTGCCCAG CCGCATGGTG 
GTGTCGATAT TCGATCCTCC TGTGAATTGG CTTCCTCCTG GTTATGTGGT AAACCAAGAC 
AAAAGTAACT CAGAGAAGTG GGAAAAGGAT GAAATGACAA AAGACTGCTT GCTGGCTAAT 
GGCAAACTGG GTGAGGCCTG TGAGGAGGAG GAGGACCTGA CGTGGAGGGC CCCCAAGGAG 
GAGGCCGAGG ATGAGGACGA CTTCCTGGAG TATGACCAGG AGCATATCCA GTTTATAGAC 
AGCATGCTCA TGGGCTCGGG CGCCTTCGTG AGGAAAATCT CTCTGTCGCC CTTCTCAGCC 
TCCGACTCTG CATACGAATG GAAGATGCCC AAGAAGGCCT CCTTGGGCAG TATGCCGTTT 
GCCTCGGGCC TTGAGGATTT TGATTACAGC TCTTGGGATG CGATGTGCTA TCTGGATCCT 
AGCAAAGCCG TTGAAGAGGA TGACTTTGTG GTAGGTTTCT GGAATCCATC AGAAGAGAAC 
TGTGGCGTCG ACACAGGGAA GCAGTCCATT TCTTACGACT TGCACACGGA GCAGTGCATT 
GCTGACAAGA GCATAGCGGA CTGTGTGGAG GCCCTGCTGG GCTGCTACTT AACCAGCTGC 
GGCGAGAGGG CTGCTCAGCT CTTCCTCTGT TCGCTGGGGC TGAAGGTGCT CCCAGTAATC 
AAAAGGACCA GCCGGGAGAA GGCCTTGGAC CCTGCTCAGG AGAACGGAAG CAGCCAACAA 
AAGAGCCTTT CAGGGAGCTG TGCCTCTCCA GTTGGCCCAC GCTCCTCTGC AGGGAAAGAC 
TTGGAGTACG GCTGCCTGAA GATCCCACCG AGGTGCATGT TTGACCATCC AGACGCAGAG 
AAAACCCTCA ACCACCTGAT ATCTGGGTTT GAAACTTTTG AGAAGAAAAT TAACTATAGA 
TTCAAGAATA AGGCTTATCT TCTGCAGGCT TTTACACACG CCTCCTACCA CTACAACACT 
ATCACTGATT GTTACCAGCG CTTAGAATTC CTGGGAGATG CGATTTTGGA CTACCTCATA 
ACCAAGCACC TTTATGAAGA CCCACGGCAG CATTCTCCAG GGGTCTTGAC TGACTTGCGC 
TCTGCCCTTG TCAATAACAC CATCTTTGCA TCATTGGCTG TGAAGTATGA CTACCACAAG 
TACTTTAAAG CTGTCTCTCC TGAGCTCTTT CATGTTATTG ATGACTTTGT GAAGTTTCAG 
CTTGAGAAGA ACGAAATGCA AGGAATGGAC TCTGAGAAAA GTTTTCTGCA AATGTGCCTC 
GTTCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dicer1 ( XM_017314998.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170487.1 Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X5, mRNA.

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CloneID	OMu78173
Clone ID Related Accession (Same CDS sequence)	XM_017314998.1 , XM_017314998.2
Accession Version	XM_017314998.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTGCT ATGTCGCCTT GACTGTTTTG AAAAATGGTT ACTTATCACT GTCAGACATT 
AACCTTTTGG TGTTTGATGA GTGTCATCTT GCGATTCTAG ACCACCCCTA TCGTGAAATT 
ATGAAGCTCT GTGAGAGTTG TCCATCATGC CCTCGAATTT TGGGACTAAC TGCTTCCATT 
TTAAATGGGA AATGTGATCC AGAGGAATTG GAAGAGAAGA TTCAGAAACT GGAGCGAATT 
CTCAGGAGTG ACGCTGAGAC GGCGACTGAC TTGGTGGTCC TGGACAGATA CACTTCTCAG 
CCTTGTGAGA TTGTGGTAGA CTGTGGACCG TTTACTGACA GAAGTGGGCT GTATGAGAGA 
TTGCTGATGG AGTTAGAAGC AGCACTTGAT TTTATCAATG ATTGTAATGT AGCTGTACAT 
TCAAAGGAAA GAGATTCTAC TTTGATTTCC AAACAAATCC TCTCAGACTG CCGTGCAGTG 
TTGGTTGTTC TGGGACCCTG GTGTGCAGAT AAAGTAGCTG GGATGATGGT AAGAGAACTT 
CAGAAGTACA TCAAACATGA GCAAGAGGAG CTACACAGGA AGTTCCTATT GTTTACAGAC 
ACCTTGTTAA GGAAAATACA CGCCCTGTGT GAAGAGTACT TCTCACCTGC CTCACTTGAC 
CTGAAGTATG TAACTCCCAA AGTCATGAAA CTGCTCGAGA TCCTGCGCAA ATACAAGCCC 
TATGAGCGAC AGCAGTTTGA AAGTGTTGAG TGGTACAATA ACAGGAATCA GGATAATTAC 
GTGTCCTGGA GTGATTCCGA GGATGACGAT GATGACGAAG AAATTGAAGA AAAAGAAAAG 
CCAGAGACAA ATTTTCCTTC TCCGTTTACC AATATTTTAT GTGGGATTAT TTTTGTGGAA 
AGAAGATACA CAGCAGTTGT CCTAAACAGA TTGATAAAGG AAGCTGGCAA ACAAGATCCA 
GAGCTGGCTT ATATCAGCAG CAACTTTATA ACTGGACACG GCATTGGAAA GAACCAGCCT 
CGCAGCAAAC AGATGGAGGC GGAGTTCAGA AAGCAGGAGG AGGTACTTAG GAAATTTCGA 
GCTCATGAGA CCAACCTGCT CATTGCAACA AGTGTTGTAG AAGAGGGTGT GGATATTCCC 
AAGTGCAATT TGGTGGTTCG TTTTGATTTG CCTACAGAGT ATCGATCATA TGTCCAGTCT 
AAAGGACGGG CACGAGCGCC GATCTCTAAT TACGTAATGT TAGCAGATAC AGACAAGATA 
AAGAGCTTTG AAGAAGACCT CAAGACCTAC AAAGCTATTG AAAAGATCTT AAGGAACAAG 
TGTTCGAAGT CAGCGGATGG TGCTGAGGCG GACGTCCATG CTGGTGTAGA TGATGAGGAT 
GCTTTCCCGC CATATGTGCT GAGGCCTGAT GATGGGGGTC CTCGAGTCAC AATCAACACT 
GCCATTGGAC ACATCAACAG ATATTGTGCT CGATTACCAA GTGATCCGTT TACGCATCTA 
GCTCCCAAAT GTAGAACCCG AGAATTGCCT GATGGTACAT TTTATTCAAC TCTTTATCTT 
CCGATTAACT CACCTCTTCG AGCCTCCATT GTTGGTCCAC CGATGGACAG TGTACGGTTG 
GCTGAAAGAG TGGTAGCTCT CATTTGCTGT GAAAAACTGC ACAAAATTGG TGAACTGGAT 
GAACACTTGA TGCCAGTTGG GAAAGAGACT GTTAAATATG AAGAAGAGCT GGACTTACAT 
GACGAGGAGG AGACCAGTGT TCCCGGGAGA CCAGGGTCCA CAAAACGACG GCAGTGCTAC 
CCCAAAGCAA TTCCAGAGTG CTTGAGGGAG AGTTACCCCA AACCCGACCA GCCTTGTTAC 
CTGTATGTGA TAGGAATGGT CTTAACTACC CCCCTCCCTG ATGAACTCAA CTTCAGGCGG 
CGGAAGCTCT ACCCACCTGA GGACACCACA AGATGCTTTG GGATCCTGAC AGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTGTAT ACACGCTCTG GAGAGGTCAC CATATCCATC 
GAGCTGAAGA AGTCTGGTTT CACATTGTCT CAACAAATGC TCGAGCTGAT TACAAGATTG 
CACCAGTACA TATTCTCACA TATTCTCCGG CTTGAGAAGC CTGCCCTGGA GTTTAAACCT 
ACAGGCGCGG AGTCAGCCTA CTGTGTTCTA CCTCTTAATG TTGTTAATGA CTCCGGCACT 
TTGGACATTG ACTTTAAATT CATGGAGGAT ATTGAGAAGT CGGAAGCTCG GATAGGCATT 
CCTAGCACCA AGTATTCAAA GGAAACACCG TTTGTCTTTA AGTTAGAAGA TTACCAAGAT 
GCAGTGATCA TTCCAAGATA CCGCAATTTT GATCAGCCTC ATCGATTTTA TGTAGCTGAT 
GTATATACGG ATCTTACCCC ACTCAGTAAA TTTCCTTCCC CTGAATATGA AACTTTTGCA 
GAATATTATA AAACAAAGTA TAACCTTGAC CTGACCAATC TCAACCAGCC ACTGCTGGAT 
GTTGACCACA CATCTTCCAG ACTGAACCTT TTGACACCTC GGCATTTGAA TCAGAAGGGG 
AAAGCACTTC CTCTAAGCAG TGCTGAGAAG AGGAAGGCCA AATGGGAAAG TCTGCAGAAC 
AAACAGATCC TGGTTCCGGA ACTCTGTGCT ATACATCCAA TCCCAGCATC ACTGTGGAGA 
AAAGCGGTTT GTCTCCCCAG CATACTGTAT CGCCTTCACT GCCTTCTGAC TGCAGAGGAA 
CTAAGAGCTC AGACAGCCAG CGATGCTGGT GTGGGTGTCA GATCACTTCC CGTGGATTTT 
AGATACCCTA ACTTAGACTT CGGGTGGAAA AAATCTATTG ATAGCAAGTC TTTCATCTCA 
AGCTGTAACT CCTCGTTGGC TGAGAGTGAT AATTACTGTA AGCACAGCAC AACTGTAGTC 
CCTGAACATG CTGCACATCA AGGTGCTACT AGACCCTCCT TAGAAAACCA TGACCAAATG 
TCTGTGAACT GCAAAAGGTT GCCCGCTGAG TCACCTGCTA AGCTCCAAAG TGAGGTTTCA 
ACGGATCTTA CAGCAATTAA TGGTCTTTCT TACAATAAAA ACCTTGCCAA TGGCAGTTAT 
GATTTAGTTA ACAGAGACTT TTGCCAAGGA AATCAGCTGA ATTACTTCAA GCAGGAAATA 
CCTGTACAAC CAACTACCTC ATATCCCATT CAGAATTTGT ACAATTATGA GAACCAGCCC 
AAGCCCAGCA ATGAATGTCC TCTACTGAGT AATACGTACC TTGATGGGAA CGCTAACACA 
TCTACCTCAG ATGGAAGCCC CGCGGTGTCC ACGATGCCTG CCATGATGAA TGCTGTTAAA 
GCACTCAAGG ACAGGATGGA CTCTGAGCAG AGCCCTTCTG TTGGGTACTC CTCAAGGACT 
CTTGGCCCCA ATCCGGGACT GATTCTCCAG GCTTTGACAC TGTCAAATGC CAGTGATGGG 
TTTAACCTGG AGCGGCTTGA GATGCTTGGC GACTCCTTTT TAAAGCATGC TATCACCACA 
TATCTGTTTT GCACGTACCC TGATGCTCAT GAAGGCCGCC TTTCATATAT GAGAAGCAAA 
AAGGTCAGCA ACTGTAACCT GTATCGCCTT GGCAAGAAGA AGGGACTGCC CAGCCGCATG 
GTGGTGTCGA TATTCGATCC TCCTGTGAAT TGGCTTCCTC CTGGTTATGT GGTAAACCAA 
GACAAAAGTA ACTCAGAGAA GTGGGAAAAG GATGAAATGA CAAAAGACTG CTTGCTGGCT 
AATGGCAAAC TGGGTGAGGC CTGTGAGGAG GAGGAGGACC TGACGTGGAG GGCCCCCAAG 
GAGGAGGCCG AGGATGAGGA CGACTTCCTG GAGTATGACC AGGAGCATAT CCAGTTTATA 
GACAGCATGC TCATGGGCTC GGGCGCCTTC GTGAGGAAAA TCTCTCTGTC GCCCTTCTCA 
GCCTCCGACT CTGCATACGA ATGGAAGATG CCCAAGAAGG CCTCCTTGGG CAGTATGCCG 
TTTGCCTCGG GCCTTGAGGA TTTTGATTAC AGCTCTTGGG ATGCGATGTG CTATCTGGAT 
CCTAGCAAAG CCGTTGAAGA GGATGACTTT GTGGTAGGTT TCTGGAATCC ATCAGAAGAG 
AACTGTGGCG TCGACACAGG GAAGCAGTCC ATTTCTTACG ACTTGCACAC GGAGCAGTGC 
ATTGCTGACA AGAGCATAGC GGACTGTGTG GAGGCCCTGC TGGGCTGCTA CTTAACCAGC 
TGCGGCGAGA GGGCTGCTCA GCTCTTCCTC TGTTCGCTGG GGCTGAAGGT GCTCCCAGTA 
ATCAAAAGGA CCAGCCGGGA GAAGGCCTTG GACCCTGCTC AGGAGAACGG AAGCAGCCAA 
CAAAAGAGCC TTTCAGGGAG CTGTGCCTCT CCAGTTGGCC CACGCTCCTC TGCAGGGAAA 
GACTTGGAGT ACGGCTGCCT GAAGATCCCA CCGAGGTGCA TGTTTGACCA TCCAGACGCA 
GAGAAAACCC TCAACCACCT GATATCTGGG TTTGAAACTT TTGAGAAGAA AATTAACTAT 
AGATTCAAGA ATAAGGCTTA TCTTCTGCAG GCTTTTACAC ACGCCTCCTA CCACTACAAC 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCACGG CAGCATTCTC CAGGGGTCTT GACTGACTTG 
CGCTCTGCCC TTGTCAATAA CACCATCTTT GCATCATTGG CTGTGAAGTA TGACTACCAC 
AAGTACTTTA AAGCTGTCTC TCCTGAGCTC TTTCATGTTA TTGATGACTT TGTGAAGTTT 
CAGCTTGAGA AGAACGAAAT GCAAGGAATG GACTCTGAGC TTAGGAGATC CGAGGAGGAT 
GAGGAGAAAG AAGAGGATAT CGAAGTCCCC AAGGCCATGG GGGACATTTT TGAGTCTCTT 
GCTGGTGCCA TTTATATGGA CAGTGGGATG TCTCTGGAGG TGGTCTGGCA GGTGTACTAT 
CCGATGATGC AGCCTCTAAT AGAAAAGTTT TCTGCAAATG TGCCTCGTTC TCCAGTGAGA 
GAATTGCTCG AGATGGAACC AGAAACTGCC AAGTTTAGCC CAGCGGAGAG AACTTATGAT 
GGAAAGGTCA GAGTCACCGT GGAAGTGGTG GGAAAGGGGA AATTTAAAGG TGTTGGCCGG 
AGCTACAGGA TCGCCAAGTC TGCTGCAGCA CGAAGGGCCC TCCGCAGCCT CAAAGCTAAC 
CAGCCTCAGG TTCCTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170487.1
CDS	106..5409
Translation	MTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_017314998.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314998.1

ORF Insert Sequence:

ATGACTTGCT ATGTCGCCTT GACTGTTTTG AAAAATGGTT ACTTATCACT GTCAGACATT 
AACCTTTTGG TGTTTGATGA GTGTCATCTT GCGATTCTAG ACCACCCCTA TCGTGAAATT 
ATGAAGCTCT GTGAGAGTTG TCCATCATGC CCTCGAATTT TGGGACTAAC TGCTTCCATT 
TTAAATGGGA AATGTGATCC AGAGGAATTG GAAGAGAAGA TTCAGAAACT GGAGCGAATT 
CTCAGGAGTG ACGCTGAGAC GGCGACTGAC TTGGTGGTCC TGGACAGATA CACTTCTCAG 
CCTTGTGAGA TTGTGGTAGA CTGTGGACCG TTTACTGACA GAAGTGGGCT GTATGAGAGA 
TTGCTGATGG AGTTAGAAGC AGCACTTGAT TTTATCAATG ATTGTAATGT AGCTGTACAT 
TCAAAGGAAA GAGATTCTAC TTTGATTTCC AAACAAATCC TCTCAGACTG CCGTGCAGTG 
TTGGTTGTTC TGGGACCCTG GTGTGCAGAT AAAGTAGCTG GGATGATGGT AAGAGAACTT 
CAGAAGTACA TCAAACATGA GCAAGAGGAG CTACACAGGA AGTTCCTATT GTTTACAGAC 
ACCTTGTTAA GGAAAATACA CGCCCTGTGT GAAGAGTACT TCTCACCTGC CTCACTTGAC 
CTGAAGTATG TAACTCCCAA AGTCATGAAA CTGCTCGAGA TCCTGCGCAA ATACAAGCCC 
TATGAGCGAC AGCAGTTTGA AAGTGTTGAG TGGTACAATA ACAGGAATCA GGATAATTAC 
GTGTCCTGGA GTGATTCCGA GGATGACGAT GATGACGAAG AAATTGAAGA AAAAGAAAAG 
CCAGAGACAA ATTTTCCTTC TCCGTTTACC AATATTTTAT GTGGGATTAT TTTTGTGGAA 
AGAAGATACA CAGCAGTTGT CCTAAACAGA TTGATAAAGG AAGCTGGCAA ACAAGATCCA 
GAGCTGGCTT ATATCAGCAG CAACTTTATA ACTGGACACG GCATTGGAAA GAACCAGCCT 
CGCAGCAAAC AGATGGAGGC GGAGTTCAGA AAGCAGGAGG AGGTACTTAG GAAATTTCGA 
GCTCATGAGA CCAACCTGCT CATTGCAACA AGTGTTGTAG AAGAGGGTGT GGATATTCCC 
AAGTGCAATT TGGTGGTTCG TTTTGATTTG CCTACAGAGT ATCGATCATA TGTCCAGTCT 
AAAGGACGGG CACGAGCGCC GATCTCTAAT TACGTAATGT TAGCAGATAC AGACAAGATA 
AAGAGCTTTG AAGAAGACCT CAAGACCTAC AAAGCTATTG AAAAGATCTT AAGGAACAAG 
TGTTCGAAGT CAGCGGATGG TGCTGAGGCG GACGTCCATG CTGGTGTAGA TGATGAGGAT 
GCTTTCCCGC CATATGTGCT GAGGCCTGAT GATGGGGGTC CTCGAGTCAC AATCAACACT 
GCCATTGGAC ACATCAACAG ATATTGTGCT CGATTACCAA GTGATCCGTT TACGCATCTA 
GCTCCCAAAT GTAGAACCCG AGAATTGCCT GATGGTACAT TTTATTCAAC TCTTTATCTT 
CCGATTAACT CACCTCTTCG AGCCTCCATT GTTGGTCCAC CGATGGACAG TGTACGGTTG 
GCTGAAAGAG TGGTAGCTCT CATTTGCTGT GAAAAACTGC ACAAAATTGG TGAACTGGAT 
GAACACTTGA TGCCAGTTGG GAAAGAGACT GTTAAATATG AAGAAGAGCT GGACTTACAT 
GACGAGGAGG AGACCAGTGT TCCCGGGAGA CCAGGGTCCA CAAAACGACG GCAGTGCTAC 
CCCAAAGCAA TTCCAGAGTG CTTGAGGGAG AGTTACCCCA AACCCGACCA GCCTTGTTAC 
CTGTATGTGA TAGGAATGGT CTTAACTACC CCCCTCCCTG ATGAACTCAA CTTCAGGCGG 
CGGAAGCTCT ACCCACCTGA GGACACCACA AGATGCTTTG GGATCCTGAC AGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTGTAT ACACGCTCTG GAGAGGTCAC CATATCCATC 
GAGCTGAAGA AGTCTGGTTT CACATTGTCT CAACAAATGC TCGAGCTGAT TACAAGATTG 
CACCAGTACA TATTCTCACA TATTCTCCGG CTTGAGAAGC CTGCCCTGGA GTTTAAACCT 
ACAGGCGCGG AGTCAGCCTA CTGTGTTCTA CCTCTTAATG TTGTTAATGA CTCCGGCACT 
TTGGACATTG ACTTTAAATT CATGGAGGAT ATTGAGAAGT CGGAAGCTCG GATAGGCATT 
CCTAGCACCA AGTATTCAAA GGAAACACCG TTTGTCTTTA AGTTAGAAGA TTACCAAGAT 
GCAGTGATCA TTCCAAGATA CCGCAATTTT GATCAGCCTC ATCGATTTTA TGTAGCTGAT 
GTATATACGG ATCTTACCCC ACTCAGTAAA TTTCCTTCCC CTGAATATGA AACTTTTGCA 
GAATATTATA AAACAAAGTA TAACCTTGAC CTGACCAATC TCAACCAGCC ACTGCTGGAT 
GTTGACCACA CATCTTCCAG ACTGAACCTT TTGACACCTC GGCATTTGAA TCAGAAGGGG 
AAAGCACTTC CTCTAAGCAG TGCTGAGAAG AGGAAGGCCA AATGGGAAAG TCTGCAGAAC 
AAACAGATCC TGGTTCCGGA ACTCTGTGCT ATACATCCAA TCCCAGCATC ACTGTGGAGA 
AAAGCGGTTT GTCTCCCCAG CATACTGTAT CGCCTTCACT GCCTTCTGAC TGCAGAGGAA 
CTAAGAGCTC AGACAGCCAG CGATGCTGGT GTGGGTGTCA GATCACTTCC CGTGGATTTT 
AGATACCCTA ACTTAGACTT CGGGTGGAAA AAATCTATTG ATAGCAAGTC TTTCATCTCA 
AGCTGTAACT CCTCGTTGGC TGAGAGTGAT AATTACTGTA AGCACAGCAC AACTGTAGTC 
CCTGAACATG CTGCACATCA AGGTGCTACT AGACCCTCCT TAGAAAACCA TGACCAAATG 
TCTGTGAACT GCAAAAGGTT GCCCGCTGAG TCACCTGCTA AGCTCCAAAG TGAGGTTTCA 
ACGGATCTTA CAGCAATTAA TGGTCTTTCT TACAATAAAA ACCTTGCCAA TGGCAGTTAT 
GATTTAGTTA ACAGAGACTT TTGCCAAGGA AATCAGCTGA ATTACTTCAA GCAGGAAATA 
CCTGTACAAC CAACTACCTC ATATCCCATT CAGAATTTGT ACAATTATGA GAACCAGCCC 
AAGCCCAGCA ATGAATGTCC TCTACTGAGT AATACGTACC TTGATGGGAA CGCTAACACA 
TCTACCTCAG ATGGAAGCCC CGCGGTGTCC ACGATGCCTG CCATGATGAA TGCTGTTAAA 
GCACTCAAGG ACAGGATGGA CTCTGAGCAG AGCCCTTCTG TTGGGTACTC CTCAAGGACT 
CTTGGCCCCA ATCCGGGACT GATTCTCCAG GCTTTGACAC TGTCAAATGC CAGTGATGGG 
TTTAACCTGG AGCGGCTTGA GATGCTTGGC GACTCCTTTT TAAAGCATGC TATCACCACA 
TATCTGTTTT GCACGTACCC TGATGCTCAT GAAGGCCGCC TTTCATATAT GAGAAGCAAA 
AAGGTCAGCA ACTGTAACCT GTATCGCCTT GGCAAGAAGA AGGGACTGCC CAGCCGCATG 
GTGGTGTCGA TATTCGATCC TCCTGTGAAT TGGCTTCCTC CTGGTTATGT GGTAAACCAA 
GACAAAAGTA ACTCAGAGAA GTGGGAAAAG GATGAAATGA CAAAAGACTG CTTGCTGGCT 
AATGGCAAAC TGGGTGAGGC CTGTGAGGAG GAGGAGGACC TGACGTGGAG GGCCCCCAAG 
GAGGAGGCCG AGGATGAGGA CGACTTCCTG GAGTATGACC AGGAGCATAT CCAGTTTATA 
GACAGCATGC TCATGGGCTC GGGCGCCTTC GTGAGGAAAA TCTCTCTGTC GCCCTTCTCA 
GCCTCCGACT CTGCATACGA ATGGAAGATG CCCAAGAAGG CCTCCTTGGG CAGTATGCCG 
TTTGCCTCGG GCCTTGAGGA TTTTGATTAC AGCTCTTGGG ATGCGATGTG CTATCTGGAT 
CCTAGCAAAG CCGTTGAAGA GGATGACTTT GTGGTAGGTT TCTGGAATCC ATCAGAAGAG 
AACTGTGGCG TCGACACAGG GAAGCAGTCC ATTTCTTACG ACTTGCACAC GGAGCAGTGC 
ATTGCTGACA AGAGCATAGC GGACTGTGTG GAGGCCCTGC TGGGCTGCTA CTTAACCAGC 
TGCGGCGAGA GGGCTGCTCA GCTCTTCCTC TGTTCGCTGG GGCTGAAGGT GCTCCCAGTA 
ATCAAAAGGA CCAGCCGGGA GAAGGCCTTG GACCCTGCTC AGGAGAACGG AAGCAGCCAA 
CAAAAGAGCC TTTCAGGGAG CTGTGCCTCT CCAGTTGGCC CACGCTCCTC TGCAGGGAAA 
GACTTGGAGT ACGGCTGCCT GAAGATCCCA CCGAGGTGCA TGTTTGACCA TCCAGACGCA 
GAGAAAACCC TCAACCACCT GATATCTGGG TTTGAAACTT TTGAGAAGAA AATTAACTAT 
AGATTCAAGA ATAAGGCTTA TCTTCTGCAG GCTTTTACAC ACGCCTCCTA CCACTACAAC 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCACGG CAGCATTCTC CAGGGGTCTT GACTGACTTG 
CGCTCTGCCC TTGTCAATAA CACCATCTTT GCATCATTGG CTGTGAAGTA TGACTACCAC 
AAGTACTTTA AAGCTGTCTC TCCTGAGCTC TTTCATGTTA TTGATGACTT TGTGAAGTTT 
CAGCTTGAGA AGAACGAAAT GCAAGGAATG GACTCTGAGC TTAGGAGATC CGAGGAGGAT 
GAGGAGAAAG AAGAGGATAT CGAAGTCCCC AAGGCCATGG GGGACATTTT TGAGTCTCTT 
GCTGGTGCCA TTTATATGGA CAGTGGGATG TCTCTGGAGG TGGTCTGGCA GGTGTACTAT 
CCGATGATGC AGCCTCTAAT AGAAAAGTTT TCTGCAAATG TGCCTCGTTC TCCAGTGAGA 
GAATTGCTCG AGATGGAACC AGAAACTGCC AAGTTTAGCC CAGCGGAGAG AACTTATGAT 
GGAAAGGTCA GAGTCACCGT GGAAGTGGTG GGAAAGGGGA AATTTAAAGG TGTTGGCCGG 
AGCTACAGGA TCGCCAAGTC TGCTGCAGCA CGAAGGGCCC TCCGCAGCCT CAAAGCTAAC 
CAGCCTCAGG TTCCTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dicer1 ( XM_017314998.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170487.1 Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X5, mRNA.

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CloneID	OMu78173
Clone ID Related Accession (Same CDS sequence)	XM_017314998.1 , XM_017314998.2
Accession Version	XM_017314998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	endoribonuclease Dicer isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTGCT ATGTCGCCTT GACTGTTTTG AAAAATGGTT ACTTATCACT GTCAGACATT 
AACCTTTTGG TGTTTGATGA GTGTCATCTT GCGATTCTAG ACCACCCCTA TCGTGAAATT 
ATGAAGCTCT GTGAGAGTTG TCCATCATGC CCTCGAATTT TGGGACTAAC TGCTTCCATT 
TTAAATGGGA AATGTGATCC AGAGGAATTG GAAGAGAAGA TTCAGAAACT GGAGCGAATT 
CTCAGGAGTG ACGCTGAGAC GGCGACTGAC TTGGTGGTCC TGGACAGATA CACTTCTCAG 
CCTTGTGAGA TTGTGGTAGA CTGTGGACCG TTTACTGACA GAAGTGGGCT GTATGAGAGA 
TTGCTGATGG AGTTAGAAGC AGCACTTGAT TTTATCAATG ATTGTAATGT AGCTGTACAT 
TCAAAGGAAA GAGATTCTAC TTTGATTTCC AAACAAATCC TCTCAGACTG CCGTGCAGTG 
TTGGTTGTTC TGGGACCCTG GTGTGCAGAT AAAGTAGCTG GGATGATGGT AAGAGAACTT 
CAGAAGTACA TCAAACATGA GCAAGAGGAG CTACACAGGA AGTTCCTATT GTTTACAGAC 
ACCTTGTTAA GGAAAATACA CGCCCTGTGT GAAGAGTACT TCTCACCTGC CTCACTTGAC 
CTGAAGTATG TAACTCCCAA AGTCATGAAA CTGCTCGAGA TCCTGCGCAA ATACAAGCCC 
TATGAGCGAC AGCAGTTTGA AAGTGTTGAG TGGTACAATA ACAGGAATCA GGATAATTAC 
GTGTCCTGGA GTGATTCCGA GGATGACGAT GATGACGAAG AAATTGAAGA AAAAGAAAAG 
CCAGAGACAA ATTTTCCTTC TCCGTTTACC AATATTTTAT GTGGGATTAT TTTTGTGGAA 
AGAAGATACA CAGCAGTTGT CCTAAACAGA TTGATAAAGG AAGCTGGCAA ACAAGATCCA 
GAGCTGGCTT ATATCAGCAG CAACTTTATA ACTGGACACG GCATTGGAAA GAACCAGCCT 
CGCAGCAAAC AGATGGAGGC GGAGTTCAGA AAGCAGGAGG AGGTACTTAG GAAATTTCGA 
GCTCATGAGA CCAACCTGCT CATTGCAACA AGTGTTGTAG AAGAGGGTGT GGATATTCCC 
AAGTGCAATT TGGTGGTTCG TTTTGATTTG CCTACAGAGT ATCGATCATA TGTCCAGTCT 
AAAGGACGGG CACGAGCGCC GATCTCTAAT TACGTAATGT TAGCAGATAC AGACAAGATA 
AAGAGCTTTG AAGAAGACCT CAAGACCTAC AAAGCTATTG AAAAGATCTT AAGGAACAAG 
TGTTCGAAGT CAGCGGATGG TGCTGAGGCG GACGTCCATG CTGGTGTAGA TGATGAGGAT 
GCTTTCCCGC CATATGTGCT GAGGCCTGAT GATGGGGGTC CTCGAGTCAC AATCAACACT 
GCCATTGGAC ACATCAACAG ATATTGTGCT CGATTACCAA GTGATCCGTT TACGCATCTA 
GCTCCCAAAT GTAGAACCCG AGAATTGCCT GATGGTACAT TTTATTCAAC TCTTTATCTT 
CCGATTAACT CACCTCTTCG AGCCTCCATT GTTGGTCCAC CGATGGACAG TGTACGGTTG 
GCTGAAAGAG TGGTAGCTCT CATTTGCTGT GAAAAACTGC ACAAAATTGG TGAACTGGAT 
GAACACTTGA TGCCAGTTGG GAAAGAGACT GTTAAATATG AAGAAGAGCT GGACTTACAT 
GACGAGGAGG AGACCAGTGT TCCCGGGAGA CCAGGGTCCA CAAAACGACG GCAGTGCTAC 
CCCAAAGCAA TTCCAGAGTG CTTGAGGGAG AGTTACCCCA AACCCGACCA GCCTTGTTAC 
CTGTATGTGA TAGGAATGGT CTTAACTACC CCCCTCCCTG ATGAACTCAA CTTCAGGCGG 
CGGAAGCTCT ACCCACCTGA GGACACCACA AGATGCTTTG GGATCCTGAC AGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTGTAT ACACGCTCTG GAGAGGTCAC CATATCCATC 
GAGCTGAAGA AGTCTGGTTT CACATTGTCT CAACAAATGC TCGAGCTGAT TACAAGATTG 
CACCAGTACA TATTCTCACA TATTCTCCGG CTTGAGAAGC CTGCCCTGGA GTTTAAACCT 
ACAGGCGCGG AGTCAGCCTA CTGTGTTCTA CCTCTTAATG TTGTTAATGA CTCCGGCACT 
TTGGACATTG ACTTTAAATT CATGGAGGAT ATTGAGAAGT CGGAAGCTCG GATAGGCATT 
CCTAGCACCA AGTATTCAAA GGAAACACCG TTTGTCTTTA AGTTAGAAGA TTACCAAGAT 
GCAGTGATCA TTCCAAGATA CCGCAATTTT GATCAGCCTC ATCGATTTTA TGTAGCTGAT 
GTATATACGG ATCTTACCCC ACTCAGTAAA TTTCCTTCCC CTGAATATGA AACTTTTGCA 
GAATATTATA AAACAAAGTA TAACCTTGAC CTGACCAATC TCAACCAGCC ACTGCTGGAT 
GTTGACCACA CATCTTCCAG ACTGAACCTT TTGACACCTC GGCATTTGAA TCAGAAGGGG 
AAAGCACTTC CTCTAAGCAG TGCTGAGAAG AGGAAGGCCA AATGGGAAAG TCTGCAGAAC 
AAACAGATCC TGGTTCCGGA ACTCTGTGCT ATACATCCAA TCCCAGCATC ACTGTGGAGA 
AAAGCGGTTT GTCTCCCCAG CATACTGTAT CGCCTTCACT GCCTTCTGAC TGCAGAGGAA 
CTAAGAGCTC AGACAGCCAG CGATGCTGGT GTGGGTGTCA GATCACTTCC CGTGGATTTT 
AGATACCCTA ACTTAGACTT CGGGTGGAAA AAATCTATTG ATAGCAAGTC TTTCATCTCA 
AGCTGTAACT CCTCGTTGGC TGAGAGTGAT AATTACTGTA AGCACAGCAC AACTGTAGTC 
CCTGAACATG CTGCACATCA AGGTGCTACT AGACCCTCCT TAGAAAACCA TGACCAAATG 
TCTGTGAACT GCAAAAGGTT GCCCGCTGAG TCACCTGCTA AGCTCCAAAG TGAGGTTTCA 
ACGGATCTTA CAGCAATTAA TGGTCTTTCT TACAATAAAA ACCTTGCCAA TGGCAGTTAT 
GATTTAGTTA ACAGAGACTT TTGCCAAGGA AATCAGCTGA ATTACTTCAA GCAGGAAATA 
CCTGTACAAC CAACTACCTC ATATCCCATT CAGAATTTGT ACAATTATGA GAACCAGCCC 
AAGCCCAGCA ATGAATGTCC TCTACTGAGT AATACGTACC TTGATGGGAA CGCTAACACA 
TCTACCTCAG ATGGAAGCCC CGCGGTGTCC ACGATGCCTG CCATGATGAA TGCTGTTAAA 
GCACTCAAGG ACAGGATGGA CTCTGAGCAG AGCCCTTCTG TTGGGTACTC CTCAAGGACT 
CTTGGCCCCA ATCCGGGACT GATTCTCCAG GCTTTGACAC TGTCAAATGC CAGTGATGGG 
TTTAACCTGG AGCGGCTTGA GATGCTTGGC GACTCCTTTT TAAAGCATGC TATCACCACA 
TATCTGTTTT GCACGTACCC TGATGCTCAT GAAGGCCGCC TTTCATATAT GAGAAGCAAA 
AAGGTCAGCA ACTGTAACCT GTATCGCCTT GGCAAGAAGA AGGGACTGCC CAGCCGCATG 
GTGGTGTCGA TATTCGATCC TCCTGTGAAT TGGCTTCCTC CTGGTTATGT GGTAAACCAA 
GACAAAAGTA ACTCAGAGAA GTGGGAAAAG GATGAAATGA CAAAAGACTG CTTGCTGGCT 
AATGGCAAAC TGGGTGAGGC CTGTGAGGAG GAGGAGGACC TGACGTGGAG GGCCCCCAAG 
GAGGAGGCCG AGGATGAGGA CGACTTCCTG GAGTATGACC AGGAGCATAT CCAGTTTATA 
GACAGCATGC TCATGGGCTC GGGCGCCTTC GTGAGGAAAA TCTCTCTGTC GCCCTTCTCA 
GCCTCCGACT CTGCATACGA ATGGAAGATG CCCAAGAAGG CCTCCTTGGG CAGTATGCCG 
TTTGCCTCGG GCCTTGAGGA TTTTGATTAC AGCTCTTGGG ATGCGATGTG CTATCTGGAT 
CCTAGCAAAG CCGTTGAAGA GGATGACTTT GTGGTAGGTT TCTGGAATCC ATCAGAAGAG 
AACTGTGGCG TCGACACAGG GAAGCAGTCC ATTTCTTACG ACTTGCACAC GGAGCAGTGC 
ATTGCTGACA AGAGCATAGC GGACTGTGTG GAGGCCCTGC TGGGCTGCTA CTTAACCAGC 
TGCGGCGAGA GGGCTGCTCA GCTCTTCCTC TGTTCGCTGG GGCTGAAGGT GCTCCCAGTA 
ATCAAAAGGA CCAGCCGGGA GAAGGCCTTG GACCCTGCTC AGGAGAACGG AAGCAGCCAA 
CAAAAGAGCC TTTCAGGGAG CTGTGCCTCT CCAGTTGGCC CACGCTCCTC TGCAGGGAAA 
GACTTGGAGT ACGGCTGCCT GAAGATCCCA CCGAGGTGCA TGTTTGACCA TCCAGACGCA 
GAGAAAACCC TCAACCACCT GATATCTGGG TTTGAAACTT TTGAGAAGAA AATTAACTAT 
AGATTCAAGA ATAAGGCTTA TCTTCTGCAG GCTTTTACAC ACGCCTCCTA CCACTACAAC 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCACGG CAGCATTCTC CAGGGGTCTT GACTGACTTG 
CGCTCTGCCC TTGTCAATAA CACCATCTTT GCATCATTGG CTGTGAAGTA TGACTACCAC 
AAGTACTTTA AAGCTGTCTC TCCTGAGCTC TTTCATGTTA TTGATGACTT TGTGAAGTTT 
CAGCTTGAGA AGAACGAAAT GCAAGGAATG GACTCTGAGC TTAGGAGATC CGAGGAGGAT 
GAGGAGAAAG AAGAGGATAT CGAAGTCCCC AAGGCCATGG GGGACATTTT TGAGTCTCTT 
GCTGGTGCCA TTTATATGGA CAGTGGGATG TCTCTGGAGG TGGTCTGGCA GGTGTACTAT 
CCGATGATGC AGCCTCTAAT AGAAAAGTTT TCTGCAAATG TGCCTCGTTC TCCAGTGAGA 
GAATTGCTCG AGATGGAACC AGAAACTGCC AAGTTTAGCC CAGCGGAGAG AACTTATGAT 
GGAAAGGTCA GAGTCACCGT GGAAGTGGTG GGAAAGGGGA AATTTAAAGG TGTTGGCCGG 
AGCTACAGGA TCGCCAAGTC TGCTGCAGCA CGAAGGGCCC TCCGCAGCCT CAAAGCTAAC 
CAGCCTCAGG TTCCTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170487.1
CDS	152..5455
Translation	MTCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_017314998.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314998.2

ORF Insert Sequence:

ATGACTTGCT ATGTCGCCTT GACTGTTTTG AAAAATGGTT ACTTATCACT GTCAGACATT 
AACCTTTTGG TGTTTGATGA GTGTCATCTT GCGATTCTAG ACCACCCCTA TCGTGAAATT 
ATGAAGCTCT GTGAGAGTTG TCCATCATGC CCTCGAATTT TGGGACTAAC TGCTTCCATT 
TTAAATGGGA AATGTGATCC AGAGGAATTG GAAGAGAAGA TTCAGAAACT GGAGCGAATT 
CTCAGGAGTG ACGCTGAGAC GGCGACTGAC TTGGTGGTCC TGGACAGATA CACTTCTCAG 
CCTTGTGAGA TTGTGGTAGA CTGTGGACCG TTTACTGACA GAAGTGGGCT GTATGAGAGA 
TTGCTGATGG AGTTAGAAGC AGCACTTGAT TTTATCAATG ATTGTAATGT AGCTGTACAT 
TCAAAGGAAA GAGATTCTAC TTTGATTTCC AAACAAATCC TCTCAGACTG CCGTGCAGTG 
TTGGTTGTTC TGGGACCCTG GTGTGCAGAT AAAGTAGCTG GGATGATGGT AAGAGAACTT 
CAGAAGTACA TCAAACATGA GCAAGAGGAG CTACACAGGA AGTTCCTATT GTTTACAGAC 
ACCTTGTTAA GGAAAATACA CGCCCTGTGT GAAGAGTACT TCTCACCTGC CTCACTTGAC 
CTGAAGTATG TAACTCCCAA AGTCATGAAA CTGCTCGAGA TCCTGCGCAA ATACAAGCCC 
TATGAGCGAC AGCAGTTTGA AAGTGTTGAG TGGTACAATA ACAGGAATCA GGATAATTAC 
GTGTCCTGGA GTGATTCCGA GGATGACGAT GATGACGAAG AAATTGAAGA AAAAGAAAAG 
CCAGAGACAA ATTTTCCTTC TCCGTTTACC AATATTTTAT GTGGGATTAT TTTTGTGGAA 
AGAAGATACA CAGCAGTTGT CCTAAACAGA TTGATAAAGG AAGCTGGCAA ACAAGATCCA 
GAGCTGGCTT ATATCAGCAG CAACTTTATA ACTGGACACG GCATTGGAAA GAACCAGCCT 
CGCAGCAAAC AGATGGAGGC GGAGTTCAGA AAGCAGGAGG AGGTACTTAG GAAATTTCGA 
GCTCATGAGA CCAACCTGCT CATTGCAACA AGTGTTGTAG AAGAGGGTGT GGATATTCCC 
AAGTGCAATT TGGTGGTTCG TTTTGATTTG CCTACAGAGT ATCGATCATA TGTCCAGTCT 
AAAGGACGGG CACGAGCGCC GATCTCTAAT TACGTAATGT TAGCAGATAC AGACAAGATA 
AAGAGCTTTG AAGAAGACCT CAAGACCTAC AAAGCTATTG AAAAGATCTT AAGGAACAAG 
TGTTCGAAGT CAGCGGATGG TGCTGAGGCG GACGTCCATG CTGGTGTAGA TGATGAGGAT 
GCTTTCCCGC CATATGTGCT GAGGCCTGAT GATGGGGGTC CTCGAGTCAC AATCAACACT 
GCCATTGGAC ACATCAACAG ATATTGTGCT CGATTACCAA GTGATCCGTT TACGCATCTA 
GCTCCCAAAT GTAGAACCCG AGAATTGCCT GATGGTACAT TTTATTCAAC TCTTTATCTT 
CCGATTAACT CACCTCTTCG AGCCTCCATT GTTGGTCCAC CGATGGACAG TGTACGGTTG 
GCTGAAAGAG TGGTAGCTCT CATTTGCTGT GAAAAACTGC ACAAAATTGG TGAACTGGAT 
GAACACTTGA TGCCAGTTGG GAAAGAGACT GTTAAATATG AAGAAGAGCT GGACTTACAT 
GACGAGGAGG AGACCAGTGT TCCCGGGAGA CCAGGGTCCA CAAAACGACG GCAGTGCTAC 
CCCAAAGCAA TTCCAGAGTG CTTGAGGGAG AGTTACCCCA AACCCGACCA GCCTTGTTAC 
CTGTATGTGA TAGGAATGGT CTTAACTACC CCCCTCCCTG ATGAACTCAA CTTCAGGCGG 
CGGAAGCTCT ACCCACCTGA GGACACCACA AGATGCTTTG GGATCCTGAC AGCCAAACCC 
ATACCTCAGA TTCCTCACTT TCCTGTGTAT ACACGCTCTG GAGAGGTCAC CATATCCATC 
GAGCTGAAGA AGTCTGGTTT CACATTGTCT CAACAAATGC TCGAGCTGAT TACAAGATTG 
CACCAGTACA TATTCTCACA TATTCTCCGG CTTGAGAAGC CTGCCCTGGA GTTTAAACCT 
ACAGGCGCGG AGTCAGCCTA CTGTGTTCTA CCTCTTAATG TTGTTAATGA CTCCGGCACT 
TTGGACATTG ACTTTAAATT CATGGAGGAT ATTGAGAAGT CGGAAGCTCG GATAGGCATT 
CCTAGCACCA AGTATTCAAA GGAAACACCG TTTGTCTTTA AGTTAGAAGA TTACCAAGAT 
GCAGTGATCA TTCCAAGATA CCGCAATTTT GATCAGCCTC ATCGATTTTA TGTAGCTGAT 
GTATATACGG ATCTTACCCC ACTCAGTAAA TTTCCTTCCC CTGAATATGA AACTTTTGCA 
GAATATTATA AAACAAAGTA TAACCTTGAC CTGACCAATC TCAACCAGCC ACTGCTGGAT 
GTTGACCACA CATCTTCCAG ACTGAACCTT TTGACACCTC GGCATTTGAA TCAGAAGGGG 
AAAGCACTTC CTCTAAGCAG TGCTGAGAAG AGGAAGGCCA AATGGGAAAG TCTGCAGAAC 
AAACAGATCC TGGTTCCGGA ACTCTGTGCT ATACATCCAA TCCCAGCATC ACTGTGGAGA 
AAAGCGGTTT GTCTCCCCAG CATACTGTAT CGCCTTCACT GCCTTCTGAC TGCAGAGGAA 
CTAAGAGCTC AGACAGCCAG CGATGCTGGT GTGGGTGTCA GATCACTTCC CGTGGATTTT 
AGATACCCTA ACTTAGACTT CGGGTGGAAA AAATCTATTG ATAGCAAGTC TTTCATCTCA 
AGCTGTAACT CCTCGTTGGC TGAGAGTGAT AATTACTGTA AGCACAGCAC AACTGTAGTC 
CCTGAACATG CTGCACATCA AGGTGCTACT AGACCCTCCT TAGAAAACCA TGACCAAATG 
TCTGTGAACT GCAAAAGGTT GCCCGCTGAG TCACCTGCTA AGCTCCAAAG TGAGGTTTCA 
ACGGATCTTA CAGCAATTAA TGGTCTTTCT TACAATAAAA ACCTTGCCAA TGGCAGTTAT 
GATTTAGTTA ACAGAGACTT TTGCCAAGGA AATCAGCTGA ATTACTTCAA GCAGGAAATA 
CCTGTACAAC CAACTACCTC ATATCCCATT CAGAATTTGT ACAATTATGA GAACCAGCCC 
AAGCCCAGCA ATGAATGTCC TCTACTGAGT AATACGTACC TTGATGGGAA CGCTAACACA 
TCTACCTCAG ATGGAAGCCC CGCGGTGTCC ACGATGCCTG CCATGATGAA TGCTGTTAAA 
GCACTCAAGG ACAGGATGGA CTCTGAGCAG AGCCCTTCTG TTGGGTACTC CTCAAGGACT 
CTTGGCCCCA ATCCGGGACT GATTCTCCAG GCTTTGACAC TGTCAAATGC CAGTGATGGG 
TTTAACCTGG AGCGGCTTGA GATGCTTGGC GACTCCTTTT TAAAGCATGC TATCACCACA 
TATCTGTTTT GCACGTACCC TGATGCTCAT GAAGGCCGCC TTTCATATAT GAGAAGCAAA 
AAGGTCAGCA ACTGTAACCT GTATCGCCTT GGCAAGAAGA AGGGACTGCC CAGCCGCATG 
GTGGTGTCGA TATTCGATCC TCCTGTGAAT TGGCTTCCTC CTGGTTATGT GGTAAACCAA 
GACAAAAGTA ACTCAGAGAA GTGGGAAAAG GATGAAATGA CAAAAGACTG CTTGCTGGCT 
AATGGCAAAC TGGGTGAGGC CTGTGAGGAG GAGGAGGACC TGACGTGGAG GGCCCCCAAG 
GAGGAGGCCG AGGATGAGGA CGACTTCCTG GAGTATGACC AGGAGCATAT CCAGTTTATA 
GACAGCATGC TCATGGGCTC GGGCGCCTTC GTGAGGAAAA TCTCTCTGTC GCCCTTCTCA 
GCCTCCGACT CTGCATACGA ATGGAAGATG CCCAAGAAGG CCTCCTTGGG CAGTATGCCG 
TTTGCCTCGG GCCTTGAGGA TTTTGATTAC AGCTCTTGGG ATGCGATGTG CTATCTGGAT 
CCTAGCAAAG CCGTTGAAGA GGATGACTTT GTGGTAGGTT TCTGGAATCC ATCAGAAGAG 
AACTGTGGCG TCGACACAGG GAAGCAGTCC ATTTCTTACG ACTTGCACAC GGAGCAGTGC 
ATTGCTGACA AGAGCATAGC GGACTGTGTG GAGGCCCTGC TGGGCTGCTA CTTAACCAGC 
TGCGGCGAGA GGGCTGCTCA GCTCTTCCTC TGTTCGCTGG GGCTGAAGGT GCTCCCAGTA 
ATCAAAAGGA CCAGCCGGGA GAAGGCCTTG GACCCTGCTC AGGAGAACGG AAGCAGCCAA 
CAAAAGAGCC TTTCAGGGAG CTGTGCCTCT CCAGTTGGCC CACGCTCCTC TGCAGGGAAA 
GACTTGGAGT ACGGCTGCCT GAAGATCCCA CCGAGGTGCA TGTTTGACCA TCCAGACGCA 
GAGAAAACCC TCAACCACCT GATATCTGGG TTTGAAACTT TTGAGAAGAA AATTAACTAT 
AGATTCAAGA ATAAGGCTTA TCTTCTGCAG GCTTTTACAC ACGCCTCCTA CCACTACAAC 
ACTATCACTG ATTGTTACCA GCGCTTAGAA TTCCTGGGAG ATGCGATTTT GGACTACCTC 
ATAACCAAGC ACCTTTATGA AGACCCACGG CAGCATTCTC CAGGGGTCTT GACTGACTTG 
CGCTCTGCCC TTGTCAATAA CACCATCTTT GCATCATTGG CTGTGAAGTA TGACTACCAC 
AAGTACTTTA AAGCTGTCTC TCCTGAGCTC TTTCATGTTA TTGATGACTT TGTGAAGTTT 
CAGCTTGAGA AGAACGAAAT GCAAGGAATG GACTCTGAGC TTAGGAGATC CGAGGAGGAT 
GAGGAGAAAG AAGAGGATAT CGAAGTCCCC AAGGCCATGG GGGACATTTT TGAGTCTCTT 
GCTGGTGCCA TTTATATGGA CAGTGGGATG TCTCTGGAGG TGGTCTGGCA GGTGTACTAT 
CCGATGATGC AGCCTCTAAT AGAAAAGTTT TCTGCAAATG TGCCTCGTTC TCCAGTGAGA 
GAATTGCTCG AGATGGAACC AGAAACTGCC AAGTTTAGCC CAGCGGAGAG AACTTATGAT 
GGAAAGGTCA GAGTCACCGT GGAAGTGGTG GGAAAGGGGA AATTTAAAGG TGTTGGCCGG 
AGCTACAGGA TCGCCAAGTC TGCTGCAGCA CGAAGGGCCC TCCGCAGCCT CAAAGCTAAC 
CAGCCTCAGG TTCCTAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Dicer1
Entrez Gene ID	192119
Full Name	dicer 1, ribonuclease type III
Synonyms	1110006F08Rik,D12Ertd7e,mKIAA0928
General protein information	Preferred Names dicer 1, ribonuclease type III Names endoribonuclease Dicer Dicer1, Dcr-1 homolog double-strand-specific ribonuclease mDCR-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 E\|12 54.83 cM

Homo sapiens (human)	DICER1	NP_085124.2
Rattus norvegicus (Norway rat)	Dicer1	XP_006240573.1
Danio rerio (zebrafish)	dicer1	NP_001154925.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002836	XP_003436256.1
Magnaporthe oryzae (rice blast fungus)	MGG_01541	XP_003714515.1
Neurospora crassa	NCU08270	XP_961898.1
	DICER1	XP_001100868.1
Gallus gallus (chicken)	DICER1	NP_001035555.1
Drosophila melanogaster (fruit fly)	Dcr-1	NP_524453.1
Caenorhabditis elegans (roundworm)	dcr-1	NP_498761.2
Xenopus tropicalis (tropical clawed frog)	dicer1	NP_001123390.2
Canis lupus familiaris (dog)	DICER1	XP_868526.3
Bos taurus (cattle)	DICER1	NP_976235.1
Schizosaccharomyces pombe (fission yeast)	dcr1	NP_588215.2
Pan troglodytes (chimpanzee)	DICER1	XP_001154369.1
Mus musculus (house mouse)	Dicer1	NP_683750.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Dicer1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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