MAN2A2 cDNA ORF clone, Bos taurus(cattle)

The following MAN2A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN2A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa38851 NM_001192641.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa228345 XM_024981808.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa228346 XM_024981809.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa228347 XM_024981811.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa228347 XM_024981810.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa228347 XM_024981812.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa228348 XM_024981814.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa30852 XM_024981815.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa38851 XM_015459187.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa38851 XM_015459188.2
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa38851 XM_015459189.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa94197 XM_015459185.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa94198 XM_015459186.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa94199 XM_015459190.1
Latest version!
Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
View Old Accession Versions >>
OBa38851 XM_015459188.1 Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa38851
    Clone ID Related Accession (Same CDS sequence) XM_015459187.1 , XM_015459188.2 , XM_015459189.1 , NM_001192641.1 , XM_015459188.1
    Accession Version NM_001192641.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000021.1. On Jul 17, 2010 this sequence version replaced XM_605840.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5721694.2037.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGGTCCGC
    GAGGCCGCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    ACCCCTGTGC TCGCCCTGCC CGTGGCCAGG AGGCAGGCCC CTGGCCCCGC TCTGCGCTCT
    TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC
    CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAACCGCGC TCCTCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGTTTCAACT GCACCACCAG CCAAGGCAAG
    GTGGCCCTGG GCAGCCTCTT CCACGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG
    ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTACGT GGAGCCCATG
    GAGATCACCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179570.1
    CDS1..3453
    Translation

    Target ORF information:

    RefSeq Version NM_001192641.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGGTCCGC
    GAGGCCGCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    ACCCCTGTGC TCGCCCTGCC CGTGGCCAGG AGGCAGGCCC CTGGCCCCGC TCTGCGCTCT
    TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC
    CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAACCGCGC TCCTCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGTTTCAACT GCACCACCAG CCAAGGCAAG
    GTGGCCCTGG GCAGCCTCTT CCACGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG
    ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTACGT GGAGCCCATG
    GAGATCACCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228345
    Clone ID Related Accession (Same CDS sequence) XM_024981808.1
    Accession Version XM_024981808.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGCCTCTC TCTCTGTCCT GCACCCAGGG GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC
    TTGGTGGACA TCCGCGACTA CGTCAACAAG GAGCTGGCCC TGCGCATTCG CACGGACATC
    AACAGCCAGG GCATCTTCTT CACGGACCTC AACGGCTTTC AGGTGCAGCC CCGACGGTAT
    CTGAAGAAGC TGCCCCTGCA GGCCAACTTC TACCCCATGC CTGTCATGGC CTACCTCCAG
    GACGCCCAGA ACCGCCTCAC GCTGCACACG GCCCAGGCCC TGGGCGTCTC CAGCCTCCAC
    GATGGCCAGC TAGAGGTGAT CTTGGACCGG AGACTGATGC AGGACGACAA CCGGGGCCTC
    GGCCAAGGGC TCAAGGACAA CAAGAGAACC TGCAACCACT TCCGCCTCCT GTTAGAACGG
    CGAACCCCAG GCAGGGAGGT CCGCGAGGCC GCCTCTGCAA GCTTCCCGTC CCTCCTCAGC
    CACCTGACCT CCATGTACCT GAACACCCCT GTGCTCGCCC TGCCCGTGGC CAGGAGGCAG
    GCCCCTGGCC CCGCTCTGCG CTCTTTTCAC CCTCTGGCCT CCTCACTGCC CTGTGACTTC
    CACCTGCTCA ACCTGCGGAC GCTCCAGGCC GAGGAGGATG GCTTGCCCTC GGCAGAAACC
    GCGCTCCTCT TACACCGCAA GGGTTTTGAC TGTGGCCTTG AGGCCAAGAA CTTGGGTTTC
    AACTGCACCA CCAGCCAAGG CAAGGTGGCC CTGGGCAGCC TCTTCCACGG CCTGGACGTG
    GTTTTCCTGC AGCCAACCTC CTTGACCCTG CTGTACCCTC TGGCCTCGCC CTCCAATAGC
    ACTGACGTCT ACGTGGAGCC CATGGAGATC ACCACCTTTC GCCTCCGCTT GGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837576.1
    CDS729..4385
    Translation

    Target ORF information:

    RefSeq Version XM_024981808.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981808.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGCCTCTC TCTCTGTCCT GCACCCAGGG GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC
    TTGGTGGACA TCCGCGACTA CGTCAACAAG GAGCTGGCCC TGCGCATTCG CACGGACATC
    AACAGCCAGG GCATCTTCTT CACGGACCTC AACGGCTTTC AGGTGCAGCC CCGACGGTAT
    CTGAAGAAGC TGCCCCTGCA GGCCAACTTC TACCCCATGC CTGTCATGGC CTACCTCCAG
    GACGCCCAGA ACCGCCTCAC GCTGCACACG GCCCAGGCCC TGGGCGTCTC CAGCCTCCAC
    GATGGCCAGC TAGAGGTGAT CTTGGACCGG AGACTGATGC AGGACGACAA CCGGGGCCTC
    GGCCAAGGGC TCAAGGACAA CAAGAGAACC TGCAACCACT TCCGCCTCCT GTTAGAACGG
    CGAACCCCAG GCAGGGAGGT CCGCGAGGCC GCCTCTGCAA GCTTCCCGTC CCTCCTCAGC
    CACCTGACCT CCATGTACCT GAACACCCCT GTGCTCGCCC TGCCCGTGGC CAGGAGGCAG
    GCCCCTGGCC CCGCTCTGCG CTCTTTTCAC CCTCTGGCCT CCTCACTGCC CTGTGACTTC
    CACCTGCTCA ACCTGCGGAC GCTCCAGGCC GAGGAGGATG GCTTGCCCTC GGCAGAAACC
    GCGCTCCTCT TACACCGCAA GGGTTTTGAC TGTGGCCTTG AGGCCAAGAA CTTGGGTTTC
    AACTGCACCA CCAGCCAAGG CAAGGTGGCC CTGGGCAGCC TCTTCCACGG CCTGGACGTG
    GTTTTCCTGC AGCCAACCTC CTTGACCCTG CTGTACCCTC TGGCCTCGCC CTCCAATAGC
    ACTGACGTCT ACGTGGAGCC CATGGAGATC ACCACCTTTC GCCTCCGCTT GGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228346
    Clone ID Related Accession (Same CDS sequence) XM_024981809.1
    Accession Version XM_024981809.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGAGTGTCC GAAGGGTGGA TGAGGAGCAG
    GAACAGCGGG TGGACATGGA GTTCCTCGTC TATGGCACCC GCTCGTCCAA AGATAAGAGT
    GGAGCCTACC TCTTTCTGCC TGACGGCGAG GCCAAGCCCT ACGTCCCCAG GGACCCACCC
    GTGCTGCGCG TCACAGAAGG CCCTTTCTTC TCAGAGGTGG TTGCCTGCTA CGAGCACGTT
    CACCAGGTGG TCCGGCTGTA TAACCTGCCG GCCTCTCTCT CTGTCCTGCA CCCAGGGGTG
    GAGGGGCTGT CCCTGGATGT GTCGTCCTTG GTGGACATCC GCGACTACGT CAACAAGGAG
    CTGGCCCTGC GCATTCGCAC GGACATCAAC AGCCAGGGCA TCTTCTTCAC GGACCTCAAC
    GGCTTTCAGG TGCAGCCCCG ACGGTATCTG AAGAAGCTGC CCCTGCAGGC CAACTTCTAC
    CCCATGCCTG TCATGGCCTA CCTCCAGGAC GCCCAGAACC GCCTCACGCT GCACACGGCC
    CAGGCCCTGG GCGTCTCCAG CCTCCACGAT GGCCAGCTAG AGGTGATCTT GGACCGGAGA
    CTGATGCAGG ACGACAACCG GGGCCTCGGC CAAGGGCTCA AGGACAACAA GAGAACCTGC
    AACCACTTCC GCCTCCTGTT AGAACGGCGA ACCCCAGGCA GGGAGGTCCG CGAGGCCGCC
    TCTGCAAGCT TCCCGTCCCT CCTCAGCCAC CTGACCTCCA TGTACCTGAA CACCCCTGTG
    CTCGCCCTGC CCGTGGCCAG GAGGCAGGCC CCTGGCCCCG CTCTGCGCTC TTTTCACCCT
    CTGGCCTCCT CACTGCCCTG TGACTTCCAC CTGCTCAACC TGCGGACGCT CCAGGCCGAG
    GAGGATGGCT TGCCCTCGGC AGAAACCGCG CTCCTCTTAC ACCGCAAGGG TTTTGACTGT
    GGCCTTGAGG CCAAGAACTT GGGTTTCAAC TGCACCACCA GCCAAGGCAA GGTGGCCCTG
    GGCAGCCTCT TCCACGGCCT GGACGTGGTT TTCCTGCAGC CAACCTCCTT GACCCTGCTG
    TACCCTCTGG CCTCGCCCTC CAATAGCACT GACGTCTACG TGGAGCCCAT GGAGATCACC
    ACCTTTCGCC TCCGCTTGGG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837577.1
    CDS729..4352
    Translation

    Target ORF information:

    RefSeq Version XM_024981809.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981809.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGAGTGTCC GAAGGGTGGA TGAGGAGCAG
    GAACAGCGGG TGGACATGGA GTTCCTCGTC TATGGCACCC GCTCGTCCAA AGATAAGAGT
    GGAGCCTACC TCTTTCTGCC TGACGGCGAG GCCAAGCCCT ACGTCCCCAG GGACCCACCC
    GTGCTGCGCG TCACAGAAGG CCCTTTCTTC TCAGAGGTGG TTGCCTGCTA CGAGCACGTT
    CACCAGGTGG TCCGGCTGTA TAACCTGCCG GCCTCTCTCT CTGTCCTGCA CCCAGGGGTG
    GAGGGGCTGT CCCTGGATGT GTCGTCCTTG GTGGACATCC GCGACTACGT CAACAAGGAG
    CTGGCCCTGC GCATTCGCAC GGACATCAAC AGCCAGGGCA TCTTCTTCAC GGACCTCAAC
    GGCTTTCAGG TGCAGCCCCG ACGGTATCTG AAGAAGCTGC CCCTGCAGGC CAACTTCTAC
    CCCATGCCTG TCATGGCCTA CCTCCAGGAC GCCCAGAACC GCCTCACGCT GCACACGGCC
    CAGGCCCTGG GCGTCTCCAG CCTCCACGAT GGCCAGCTAG AGGTGATCTT GGACCGGAGA
    CTGATGCAGG ACGACAACCG GGGCCTCGGC CAAGGGCTCA AGGACAACAA GAGAACCTGC
    AACCACTTCC GCCTCCTGTT AGAACGGCGA ACCCCAGGCA GGGAGGTCCG CGAGGCCGCC
    TCTGCAAGCT TCCCGTCCCT CCTCAGCCAC CTGACCTCCA TGTACCTGAA CACCCCTGTG
    CTCGCCCTGC CCGTGGCCAG GAGGCAGGCC CCTGGCCCCG CTCTGCGCTC TTTTCACCCT
    CTGGCCTCCT CACTGCCCTG TGACTTCCAC CTGCTCAACC TGCGGACGCT CCAGGCCGAG
    GAGGATGGCT TGCCCTCGGC AGAAACCGCG CTCCTCTTAC ACCGCAAGGG TTTTGACTGT
    GGCCTTGAGG CCAAGAACTT GGGTTTCAAC TGCACCACCA GCCAAGGCAA GGTGGCCCTG
    GGCAGCCTCT TCCACGGCCT GGACGTGGTT TTCCTGCAGC CAACCTCCTT GACCCTGCTG
    TACCCTCTGG CCTCGCCCTC CAATAGCACT GACGTCTACG TGGAGCCCAT GGAGATCACC
    ACCTTTCGCC TCCGCTTGGG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228347
    Clone ID Related Accession (Same CDS sequence) XM_024981810.1 , XM_024981811.1 , XM_024981812.1
    Accession Version XM_024981810.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGCCTCTC TCTCTGTCCT GCACCCAGGG GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC
    TTGGTGGACA TCCGCGACTA CGTCAACAAG GAGCTGGCCC TGCGCATTCG CACGGACATC
    AACAGCCAGG GCATCTTCTT CACGGACCTC AACGGCTTTC AGGTGCAGCC CCGACGGTAT
    CTGAAGAAGC TGCCCCTGCA GGCCAACTTC TACCCCATGC CTGTCATGGC CTACCTCCAG
    GACGCCCAGA ACCGCCTCAC GCTGCACACG GCCCAGGCCC TGGGCGTCTC CAGCCTCCAC
    GATGGCCAGC TAGAGGTGAT CTTGGACCGG AGACTGATGC AGGACGACAA CCGGGGCCTC
    GGCCAAGGGC TCAAGGACAA CAAGAGAACC TGCAACCACT TCCGCCTCCT GTTAGAACGG
    CGAACCCCAG GCAGGGAGGT CCGCGAGGCC GCCTCTGCAA GCTTCCCGTC CCTCCTCAGC
    CACCTGACCT CCATGTACCT GAACACCCCT GTGCTCGCCC TGCCCGTGGC CAGGAGGCAG
    GCCCCTGGCC CCGCTCTGCG CTCTTTTCAC CCTCTGGCCT CCTCACTGCC CTGTGACTTC
    CACCTGCTCA ACCTGCGGAC GCTCCAGGCC GAGGAGGATG GCTTGCCCTC GGCAGAAACC
    GCGCTCCTCT TACACCGCAA GGGTTTTGAC TGTGGCCTTG AGGCCAAGAA CTTGGGTTTC
    AACTGCACCA CCAGCCAAGG CAAGGTGGCC CTGGGCAGCC TCTTCCACGG CCTGGACGTG
    GTTTTCCTGC AGCCAACCTC CTTGACCCTG CTGTACCCTC TGGCCTCGCC CTCCAATAGC
    ACTGACGTCT ACGTGGAGCC CATGGAGATC ACCACCTTTC GCCTCCGCTT GGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837578.1
    CDS427..3903
    Translation

    Target ORF information:

    RefSeq Version XM_024981810.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981810.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGCCTCTC TCTCTGTCCT GCACCCAGGG GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC
    TTGGTGGACA TCCGCGACTA CGTCAACAAG GAGCTGGCCC TGCGCATTCG CACGGACATC
    AACAGCCAGG GCATCTTCTT CACGGACCTC AACGGCTTTC AGGTGCAGCC CCGACGGTAT
    CTGAAGAAGC TGCCCCTGCA GGCCAACTTC TACCCCATGC CTGTCATGGC CTACCTCCAG
    GACGCCCAGA ACCGCCTCAC GCTGCACACG GCCCAGGCCC TGGGCGTCTC CAGCCTCCAC
    GATGGCCAGC TAGAGGTGAT CTTGGACCGG AGACTGATGC AGGACGACAA CCGGGGCCTC
    GGCCAAGGGC TCAAGGACAA CAAGAGAACC TGCAACCACT TCCGCCTCCT GTTAGAACGG
    CGAACCCCAG GCAGGGAGGT CCGCGAGGCC GCCTCTGCAA GCTTCCCGTC CCTCCTCAGC
    CACCTGACCT CCATGTACCT GAACACCCCT GTGCTCGCCC TGCCCGTGGC CAGGAGGCAG
    GCCCCTGGCC CCGCTCTGCG CTCTTTTCAC CCTCTGGCCT CCTCACTGCC CTGTGACTTC
    CACCTGCTCA ACCTGCGGAC GCTCCAGGCC GAGGAGGATG GCTTGCCCTC GGCAGAAACC
    GCGCTCCTCT TACACCGCAA GGGTTTTGAC TGTGGCCTTG AGGCCAAGAA CTTGGGTTTC
    AACTGCACCA CCAGCCAAGG CAAGGTGGCC CTGGGCAGCC TCTTCCACGG CCTGGACGTG
    GTTTTCCTGC AGCCAACCTC CTTGACCCTG CTGTACCCTC TGGCCTCGCC CTCCAATAGC
    ACTGACGTCT ACGTGGAGCC CATGGAGATC ACCACCTTTC GCCTCCGCTT GGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228347
    Clone ID Related Accession (Same CDS sequence) XM_024981810.1 , XM_024981811.1 , XM_024981812.1
    Accession Version XM_024981811.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGCCTCTC TCTCTGTCCT GCACCCAGGG GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC
    TTGGTGGACA TCCGCGACTA CGTCAACAAG GAGCTGGCCC TGCGCATTCG CACGGACATC
    AACAGCCAGG GCATCTTCTT CACGGACCTC AACGGCTTTC AGGTGCAGCC CCGACGGTAT
    CTGAAGAAGC TGCCCCTGCA GGCCAACTTC TACCCCATGC CTGTCATGGC CTACCTCCAG
    GACGCCCAGA ACCGCCTCAC GCTGCACACG GCCCAGGCCC TGGGCGTCTC CAGCCTCCAC
    GATGGCCAGC TAGAGGTGAT CTTGGACCGG AGACTGATGC AGGACGACAA CCGGGGCCTC
    GGCCAAGGGC TCAAGGACAA CAAGAGAACC TGCAACCACT TCCGCCTCCT GTTAGAACGG
    CGAACCCCAG GCAGGGAGGT CCGCGAGGCC GCCTCTGCAA GCTTCCCGTC CCTCCTCAGC
    CACCTGACCT CCATGTACCT GAACACCCCT GTGCTCGCCC TGCCCGTGGC CAGGAGGCAG
    GCCCCTGGCC CCGCTCTGCG CTCTTTTCAC CCTCTGGCCT CCTCACTGCC CTGTGACTTC
    CACCTGCTCA ACCTGCGGAC GCTCCAGGCC GAGGAGGATG GCTTGCCCTC GGCAGAAACC
    GCGCTCCTCT TACACCGCAA GGGTTTTGAC TGTGGCCTTG AGGCCAAGAA CTTGGGTTTC
    AACTGCACCA CCAGCCAAGG CAAGGTGGCC CTGGGCAGCC TCTTCCACGG CCTGGACGTG
    GTTTTCCTGC AGCCAACCTC CTTGACCCTG CTGTACCCTC TGGCCTCGCC CTCCAATAGC
    ACTGACGTCT ACGTGGAGCC CATGGAGATC ACCACCTTTC GCCTCCGCTT GGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837579.1
    CDS729..4205
    Translation

    Target ORF information:

    RefSeq Version XM_024981811.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981811.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGCCTCTC TCTCTGTCCT GCACCCAGGG GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC
    TTGGTGGACA TCCGCGACTA CGTCAACAAG GAGCTGGCCC TGCGCATTCG CACGGACATC
    AACAGCCAGG GCATCTTCTT CACGGACCTC AACGGCTTTC AGGTGCAGCC CCGACGGTAT
    CTGAAGAAGC TGCCCCTGCA GGCCAACTTC TACCCCATGC CTGTCATGGC CTACCTCCAG
    GACGCCCAGA ACCGCCTCAC GCTGCACACG GCCCAGGCCC TGGGCGTCTC CAGCCTCCAC
    GATGGCCAGC TAGAGGTGAT CTTGGACCGG AGACTGATGC AGGACGACAA CCGGGGCCTC
    GGCCAAGGGC TCAAGGACAA CAAGAGAACC TGCAACCACT TCCGCCTCCT GTTAGAACGG
    CGAACCCCAG GCAGGGAGGT CCGCGAGGCC GCCTCTGCAA GCTTCCCGTC CCTCCTCAGC
    CACCTGACCT CCATGTACCT GAACACCCCT GTGCTCGCCC TGCCCGTGGC CAGGAGGCAG
    GCCCCTGGCC CCGCTCTGCG CTCTTTTCAC CCTCTGGCCT CCTCACTGCC CTGTGACTTC
    CACCTGCTCA ACCTGCGGAC GCTCCAGGCC GAGGAGGATG GCTTGCCCTC GGCAGAAACC
    GCGCTCCTCT TACACCGCAA GGGTTTTGAC TGTGGCCTTG AGGCCAAGAA CTTGGGTTTC
    AACTGCACCA CCAGCCAAGG CAAGGTGGCC CTGGGCAGCC TCTTCCACGG CCTGGACGTG
    GTTTTCCTGC AGCCAACCTC CTTGACCCTG CTGTACCCTC TGGCCTCGCC CTCCAATAGC
    ACTGACGTCT ACGTGGAGCC CATGGAGATC ACCACCTTTC GCCTCCGCTT GGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228347
    Clone ID Related Accession (Same CDS sequence) XM_024981810.1 , XM_024981811.1 , XM_024981812.1
    Accession Version XM_024981812.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGCCTCTC TCTCTGTCCT GCACCCAGGG GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC
    TTGGTGGACA TCCGCGACTA CGTCAACAAG GAGCTGGCCC TGCGCATTCG CACGGACATC
    AACAGCCAGG GCATCTTCTT CACGGACCTC AACGGCTTTC AGGTGCAGCC CCGACGGTAT
    CTGAAGAAGC TGCCCCTGCA GGCCAACTTC TACCCCATGC CTGTCATGGC CTACCTCCAG
    GACGCCCAGA ACCGCCTCAC GCTGCACACG GCCCAGGCCC TGGGCGTCTC CAGCCTCCAC
    GATGGCCAGC TAGAGGTGAT CTTGGACCGG AGACTGATGC AGGACGACAA CCGGGGCCTC
    GGCCAAGGGC TCAAGGACAA CAAGAGAACC TGCAACCACT TCCGCCTCCT GTTAGAACGG
    CGAACCCCAG GCAGGGAGGT CCGCGAGGCC GCCTCTGCAA GCTTCCCGTC CCTCCTCAGC
    CACCTGACCT CCATGTACCT GAACACCCCT GTGCTCGCCC TGCCCGTGGC CAGGAGGCAG
    GCCCCTGGCC CCGCTCTGCG CTCTTTTCAC CCTCTGGCCT CCTCACTGCC CTGTGACTTC
    CACCTGCTCA ACCTGCGGAC GCTCCAGGCC GAGGAGGATG GCTTGCCCTC GGCAGAAACC
    GCGCTCCTCT TACACCGCAA GGGTTTTGAC TGTGGCCTTG AGGCCAAGAA CTTGGGTTTC
    AACTGCACCA CCAGCCAAGG CAAGGTGGCC CTGGGCAGCC TCTTCCACGG CCTGGACGTG
    GTTTTCCTGC AGCCAACCTC CTTGACCCTG CTGTACCCTC TGGCCTCGCC CTCCAATAGC
    ACTGACGTCT ACGTGGAGCC CATGGAGATC ACCACCTTTC GCCTCCGCTT GGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837580.1
    CDS423..3899
    Translation

    Target ORF information:

    RefSeq Version XM_024981812.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981812.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGCCTCTC TCTCTGTCCT GCACCCAGGG GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC
    TTGGTGGACA TCCGCGACTA CGTCAACAAG GAGCTGGCCC TGCGCATTCG CACGGACATC
    AACAGCCAGG GCATCTTCTT CACGGACCTC AACGGCTTTC AGGTGCAGCC CCGACGGTAT
    CTGAAGAAGC TGCCCCTGCA GGCCAACTTC TACCCCATGC CTGTCATGGC CTACCTCCAG
    GACGCCCAGA ACCGCCTCAC GCTGCACACG GCCCAGGCCC TGGGCGTCTC CAGCCTCCAC
    GATGGCCAGC TAGAGGTGAT CTTGGACCGG AGACTGATGC AGGACGACAA CCGGGGCCTC
    GGCCAAGGGC TCAAGGACAA CAAGAGAACC TGCAACCACT TCCGCCTCCT GTTAGAACGG
    CGAACCCCAG GCAGGGAGGT CCGCGAGGCC GCCTCTGCAA GCTTCCCGTC CCTCCTCAGC
    CACCTGACCT CCATGTACCT GAACACCCCT GTGCTCGCCC TGCCCGTGGC CAGGAGGCAG
    GCCCCTGGCC CCGCTCTGCG CTCTTTTCAC CCTCTGGCCT CCTCACTGCC CTGTGACTTC
    CACCTGCTCA ACCTGCGGAC GCTCCAGGCC GAGGAGGATG GCTTGCCCTC GGCAGAAACC
    GCGCTCCTCT TACACCGCAA GGGTTTTGAC TGTGGCCTTG AGGCCAAGAA CTTGGGTTTC
    AACTGCACCA CCAGCCAAGG CAAGGTGGCC CTGGGCAGCC TCTTCCACGG CCTGGACGTG
    GTTTTCCTGC AGCCAACCTC CTTGACCCTG CTGTACCCTC TGGCCTCGCC CTCCAATAGC
    ACTGACGTCT ACGTGGAGCC CATGGAGATC ACCACCTTTC GCCTCCGCTT GGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228348
    Clone ID Related Accession (Same CDS sequence) XM_024981814.1
    Accession Version XM_024981814.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3303bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGCCTCTC TCTCTGTCCT GCACCCAGGG GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC
    TTGGTGGACA TCCGCGACTA CGTCAACAAG GAGCTGGCCC TGCGCATTCG CACGGACATC
    AACAGCCAGG GCATCTTCTT CACGGACCTC AACGGCTTTC AGGTGCAGCC CCGACGGTAT
    CTGAAGAAGC TGCCCCTGCA GGCCAACTTC TACCCCATGC CTGTCATGGC CTACCTCCAG
    GACGCCCAGA ACCGCCTCAC GCTGCACACG GCCCAGGCCC TGGGCGTCTC CAGCCTCCAC
    GATGGCCAGC TAGAGGTGAT CTTGGACCGG AGACTGATGC AGGACGACAA CCGGGGCCTC
    GGCCAAGGGC TCAAGGACAA CAAGAGAACC TGCAACCACT TCCGCCTCCT GTTAGAACGG
    CGAACCCCAG GCAGGGAGCC TGGCTTTTTC TCCAAACTGG CAGCCATGTT TAGGGGCTTG
    ATCTTTCACA GCAGCAGGAA CAGGAACCGA GAGGTAAGGG CCAGTGATGC GGTGTCACAC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837582.1
    CDS729..4031
    Translation

    Target ORF information:

    RefSeq Version XM_024981814.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGCCTCTC TCTCTGTCCT GCACCCAGGG GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC
    TTGGTGGACA TCCGCGACTA CGTCAACAAG GAGCTGGCCC TGCGCATTCG CACGGACATC
    AACAGCCAGG GCATCTTCTT CACGGACCTC AACGGCTTTC AGGTGCAGCC CCGACGGTAT
    CTGAAGAAGC TGCCCCTGCA GGCCAACTTC TACCCCATGC CTGTCATGGC CTACCTCCAG
    GACGCCCAGA ACCGCCTCAC GCTGCACACG GCCCAGGCCC TGGGCGTCTC CAGCCTCCAC
    GATGGCCAGC TAGAGGTGAT CTTGGACCGG AGACTGATGC AGGACGACAA CCGGGGCCTC
    GGCCAAGGGC TCAAGGACAA CAAGAGAACC TGCAACCACT TCCGCCTCCT GTTAGAACGG
    CGAACCCCAG GCAGGGAGCC TGGCTTTTTC TCCAAACTGG CAGCCATGTT TAGGGGCTTG
    ATCTTTCACA GCAGCAGGAA CAGGAACCGA GAGGTAAGGG CCAGTGATGC GGTGTCACAC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30852
    Clone ID Related Accession (Same CDS sequence) XM_024981815.1
    Accession Version XM_024981815.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGCCTGGC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG
    AACCGAGAGG TCCGCGAGGC CGCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCCGTGG CCAGGAGGCA GGCCCCTGGC
    CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAAC CGCGCTCCTC
    TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGTTT CAACTGCACC
    ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCACG GCCTGGACGT GGTTTTCCTG
    CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC
    TACGTGGAGC CCATGGAGAT CACCACCTTT CGCCTCCGCT TGGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837583.1
    CDS729..4436
    Translation

    Target ORF information:

    RefSeq Version XM_024981815.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981815.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGCCTGGC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG
    AACCGAGAGG TCCGCGAGGC CGCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCCGTGG CCAGGAGGCA GGCCCCTGGC
    CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC
    AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAAC CGCGCTCCTC
    TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGTTT CAACTGCACC
    ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCACG GCCTGGACGT GGTTTTCCTG
    CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC
    TACGTGGAGC CCATGGAGAT CACCACCTTT CGCCTCCGCT TGGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38851
    Clone ID Related Accession (Same CDS sequence) XM_015459187.1 , XM_015459188.2 , XM_015459189.1 , NM_001192641.1 , XM_015459188.1
    Accession Version XM_015459187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGGTCCGC
    GAGGCCGCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    ACCCCTGTGC TCGCCCTGCC CGTGGCCAGG AGGCAGGCCC CTGGCCCCGC TCTGCGCTCT
    TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC
    CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAACCGCGC TCCTCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGTTTCAACT GCACCACCAG CCAAGGCAAG
    GTGGCCCTGG GCAGCCTCTT CCACGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG
    ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTACGT GGAGCCCATG
    GAGATCACCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314673.1
    CDS610..4062
    Translation

    Target ORF information:

    RefSeq Version XM_015459187.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGGTCCGC
    GAGGCCGCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    ACCCCTGTGC TCGCCCTGCC CGTGGCCAGG AGGCAGGCCC CTGGCCCCGC TCTGCGCTCT
    TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC
    CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAACCGCGC TCCTCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGTTTCAACT GCACCACCAG CCAAGGCAAG
    GTGGCCCTGG GCAGCCTCTT CCACGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG
    ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTACGT GGAGCCCATG
    GAGATCACCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38851
    Clone ID Related Accession (Same CDS sequence) XM_015459187.1 , XM_015459188.2 , XM_015459189.1 , NM_001192641.1 , XM_015459188.1
    Accession Version XM_015459188.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGGTCCGC
    GAGGCCGCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    ACCCCTGTGC TCGCCCTGCC CGTGGCCAGG AGGCAGGCCC CTGGCCCCGC TCTGCGCTCT
    TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC
    CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAACCGCGC TCCTCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGTTTCAACT GCACCACCAG CCAAGGCAAG
    GTGGCCCTGG GCAGCCTCTT CCACGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG
    ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTACGT GGAGCCCATG
    GAGATCACCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314674.1
    CDS426..3878
    Translation

    Target ORF information:

    RefSeq Version XM_015459188.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459188.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGGTCCGC
    GAGGCCGCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    ACCCCTGTGC TCGCCCTGCC CGTGGCCAGG AGGCAGGCCC CTGGCCCCGC TCTGCGCTCT
    TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC
    CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAACCGCGC TCCTCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGTTTCAACT GCACCACCAG CCAAGGCAAG
    GTGGCCCTGG GCAGCCTCTT CCACGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG
    ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTACGT GGAGCCCATG
    GAGATCACCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38851
    Clone ID Related Accession (Same CDS sequence) XM_015459187.1 , XM_015459188.2 , XM_015459189.1 , NM_001192641.1 , XM_015459188.1
    Accession Version XM_015459189.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGGTCCGC
    GAGGCCGCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    ACCCCTGTGC TCGCCCTGCC CGTGGCCAGG AGGCAGGCCC CTGGCCCCGC TCTGCGCTCT
    TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC
    CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAACCGCGC TCCTCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGTTTCAACT GCACCACCAG CCAAGGCAAG
    GTGGCCCTGG GCAGCCTCTT CCACGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG
    ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTACGT GGAGCCCATG
    GAGATCACCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314675.1
    CDS304..3756
    Translation

    Target ORF information:

    RefSeq Version XM_015459189.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459189.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGGTCCGC
    GAGGCCGCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    ACCCCTGTGC TCGCCCTGCC CGTGGCCAGG AGGCAGGCCC CTGGCCCCGC TCTGCGCTCT
    TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC
    CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAACCGCGC TCCTCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGTTTCAACT GCACCACCAG CCAAGGCAAG
    GTGGCCCTGG GCAGCCTCTT CCACGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG
    ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTACGT GGAGCCCATG
    GAGATCACCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38851
    Clone ID Related Accession (Same CDS sequence) XM_015459187.1 , XM_015459188.2 , XM_015459189.1 , NM_001192641.1 , XM_015459188.1
    Accession Version XM_015459188.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGGTCCGC
    GAGGCCGCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    ACCCCTGTGC TCGCCCTGCC CGTGGCCAGG AGGCAGGCCC CTGGCCCCGC TCTGCGCTCT
    TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC
    CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAACCGCGC TCCTCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGTTTCAACT GCACCACCAG CCAAGGCAAG
    GTGGCCCTGG GCAGCCTCTT CCACGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG
    ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTACGT GGAGCCCATG
    GAGATCACCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314674.1
    CDS308..3760
    Translation

    Target ORF information:

    RefSeq Version XM_015459188.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGGTCCGC
    GAGGCCGCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    ACCCCTGTGC TCGCCCTGCC CGTGGCCAGG AGGCAGGCCC CTGGCCCCGC TCTGCGCTCT
    TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC
    CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAACCGCGC TCCTCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGTTTCAACT GCACCACCAG CCAAGGCAAG
    GTGGCCCTGG GCAGCCTCTT CCACGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG
    ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTACGT GGAGCCCATG
    GAGATCACCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94197
    Clone ID Related Accession (Same CDS sequence) XM_015459185.1
    Accession Version XM_015459185.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGGTCCGC
    GAGGCCGCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    ACCCCTGTGC TCGCCCTGCC CGTGGCCAGG AGGCAGGCCC CTGGCCCCGC TCTGCGCTCT
    TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC
    CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAACCGCGC TCCTCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGTTTCAACT GCACCACCAG CCAAGGCAAG
    GTGGCCCTGG GCAGCCTCTT CCACGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG
    ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTACGT GGAGCCCATG
    GAGATCACCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314671.1
    CDS1322..4954
    Translation

    Target ORF information:

    RefSeq Version XM_015459185.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459185.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGGTCCGC
    GAGGCCGCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC
    ACCCCTGTGC TCGCCCTGCC CGTGGCCAGG AGGCAGGCCC CTGGCCCCGC TCTGCGCTCT
    TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC
    CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAACCGCGC TCCTCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGTTTCAACT GCACCACCAG CCAAGGCAAG
    GTGGCCCTGG GCAGCCTCTT CCACGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG
    ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTACGT GGAGCCCATG
    GAGATCACCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94198
    Clone ID Related Accession (Same CDS sequence) XM_015459186.1
    Accession Version XM_015459186.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGAGTGTCC GAAGGGTGGA TGAGGAGCAG
    GAACAGCGGG TGGACATGGA GTTCCTCGTC TATGGCACCC GCTCGTCCAA AGATAAGAGT
    GGAGCCTACC TCTTTCTGCC TGACGGCGAG GCCAAGCCCT ACGTCCCCAG GGACCCACCC
    GTGCTGCGCG TCACAGAAGG CCCTTTCTTC TCAGAGGTGG TTGCCTGCTA CGAGCACGTT
    CACCAGGTGG TCCGGCTGTA TAACCTGCCG GGGGTGGAGG GGCTGTCCCT GGATGTGTCG
    TCCTTGGTGG ACATCCGCGA CTACGTCAAC AAGGAGCTGG CCCTGCGCAT TCGCACGGAC
    ATCAACAGCC AGGGCATCTT CTTCACGGAC CTCAACGGCT TTCAGGTGCA GCCCCGACGG
    TATCTGAAGA AGCTGCCCCT GCAGGCCAAC TTCTACCCCA TGCCTGTCAT GGCCTACCTC
    CAGGACGCCC AGAACCGCCT CACGCTGCAC ACGGCCCAGG CCCTGGGCGT CTCCAGCCTC
    CACGATGGCC AGCTAGAGGT GATCTTGGAC CGGAGACTGA TGCAGGACGA CAACCGGGGC
    CTCGGCCAAG GGCTCAAGGA CAACAAGAGA ACCTGCAACC ACTTCCGCCT CCTGTTAGAA
    CGGCGAACCC CAGGCAGGGA GGTCCGCGAG GCCGCCTCTG CAAGCTTCCC GTCCCTCCTC
    AGCCACCTGA CCTCCATGTA CCTGAACACC CCTGTGCTCG CCCTGCCCGT GGCCAGGAGG
    CAGGCCCCTG GCCCCGCTCT GCGCTCTTTT CACCCTCTGG CCTCCTCACT GCCCTGTGAC
    TTCCACCTGC TCAACCTGCG GACGCTCCAG GCCGAGGAGG ATGGCTTGCC CTCGGCAGAA
    ACCGCGCTCC TCTTACACCG CAAGGGTTTT GACTGTGGCC TTGAGGCCAA GAACTTGGGT
    TTCAACTGCA CCACCAGCCA AGGCAAGGTG GCCCTGGGCA GCCTCTTCCA CGGCCTGGAC
    GTGGTTTTCC TGCAGCCAAC CTCCTTGACC CTGCTGTACC CTCTGGCCTC GCCCTCCAAT
    AGCACTGACG TCTACGTGGA GCCCATGGAG ATCACCACCT TTCGCCTCCG CTTGGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314672.1
    CDS1322..4921
    Translation

    Target ORF information:

    RefSeq Version XM_015459186.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459186.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGAGTGTCC GAAGGGTGGA TGAGGAGCAG
    GAACAGCGGG TGGACATGGA GTTCCTCGTC TATGGCACCC GCTCGTCCAA AGATAAGAGT
    GGAGCCTACC TCTTTCTGCC TGACGGCGAG GCCAAGCCCT ACGTCCCCAG GGACCCACCC
    GTGCTGCGCG TCACAGAAGG CCCTTTCTTC TCAGAGGTGG TTGCCTGCTA CGAGCACGTT
    CACCAGGTGG TCCGGCTGTA TAACCTGCCG GGGGTGGAGG GGCTGTCCCT GGATGTGTCG
    TCCTTGGTGG ACATCCGCGA CTACGTCAAC AAGGAGCTGG CCCTGCGCAT TCGCACGGAC
    ATCAACAGCC AGGGCATCTT CTTCACGGAC CTCAACGGCT TTCAGGTGCA GCCCCGACGG
    TATCTGAAGA AGCTGCCCCT GCAGGCCAAC TTCTACCCCA TGCCTGTCAT GGCCTACCTC
    CAGGACGCCC AGAACCGCCT CACGCTGCAC ACGGCCCAGG CCCTGGGCGT CTCCAGCCTC
    CACGATGGCC AGCTAGAGGT GATCTTGGAC CGGAGACTGA TGCAGGACGA CAACCGGGGC
    CTCGGCCAAG GGCTCAAGGA CAACAAGAGA ACCTGCAACC ACTTCCGCCT CCTGTTAGAA
    CGGCGAACCC CAGGCAGGGA GGTCCGCGAG GCCGCCTCTG CAAGCTTCCC GTCCCTCCTC
    AGCCACCTGA CCTCCATGTA CCTGAACACC CCTGTGCTCG CCCTGCCCGT GGCCAGGAGG
    CAGGCCCCTG GCCCCGCTCT GCGCTCTTTT CACCCTCTGG CCTCCTCACT GCCCTGTGAC
    TTCCACCTGC TCAACCTGCG GACGCTCCAG GCCGAGGAGG ATGGCTTGCC CTCGGCAGAA
    ACCGCGCTCC TCTTACACCG CAAGGGTTTT GACTGTGGCC TTGAGGCCAA GAACTTGGGT
    TTCAACTGCA CCACCAGCCA AGGCAAGGTG GCCCTGGGCA GCCTCTTCCA CGGCCTGGAC
    GTGGTTTTCC TGCAGCCAAC CTCCTTGACC CTGCTGTACC CTCTGGCCTC GCCCTCCAAT
    AGCACTGACG TCTACGTGGA GCCCATGGAG ATCACCACCT TTCGCCTCCG CTTGGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94199
    Clone ID Related Accession (Same CDS sequence) XM_015459190.1
    Accession Version XM_015459190.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-mannosidase 2x isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGCCTGGC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG
    AACCGAGAGG TAAGGGCCAG TGATGCGGTG TCACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314676.1
    CDS1322..4600
    Translation

    Target ORF information:

    RefSeq Version XM_015459190.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459190.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCACCCT GCCAGGTTGT CACAGAGCAC GAGAAGCTTT TGTTTCTTTG GAGTCAGGCA 
    AATTGGTGTC CCGCAGGAGC AGGAACTCAT TGGGACATTT GGTGGAAAGA CCACCACGGG
    ATTTTTGCTT GCCGGAGCTA CATCTGCTGT CCCTCCAGCC AGGTGTGTGT GGAGGCCAGT
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC
    TCGCTCTACC TCATGTTGGA CCGAGTACAA CATGATCCTG CTCGACACCA GAACGGTGGG
    AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG
    TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACGGCC
    AACGTGGAGG GCCCACCTGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG
    GGGGGCCGCG GCCCGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GTTGCCATTT
    GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCACACGAC
    TGGGACTCCG AGGACCTGCA GGTGTTCGTG GTCCCCCACT CTCACAACGA CCCAGGCTGG
    CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG
    TCTAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTCGCC
    AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ATGAGGCCAA CTCCCATTAT
    TTTGCGTTGA TTGACCAGCT CATCGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA
    ACCCCGCGCT CAGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG
    CACTTTGCCG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGACGCC
    AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT
    GGCCCGGATC CAAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG TGGGCGGATC
    AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC CGAGAGGGCA
    GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG
    GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCACGT GCAGGCCCAG
    TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGG
    GCCCGGCCTC CAGGTTTCCC TGTGCTGAGC GGGGATTTCT TCTCCTACGC GGACCGGGAG
    GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC
    CTGGAAGCAC ACCTGCGGGG CGCAGAGATC CTGTACAGCC TGGCTGTGGC TCACGCCCGC
    CGCGCTGGAC TGGCCAGCCA GTTCCCGCTC TCGAACTTCG CCCTTCTGAC GGACGCTCGG
    CGCACACTGG GGCTCTTCCA GCACCACGAC GCCATCACAG GCACGGCCAA GGAGGCCGTT
    GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAGCA GGTCATCATG
    AATGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGACCT ACCACTTTGA CCCAGAGGTG
    CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG
    ATCCAGCTGG AATCTTCACC CAGGTATGTG GTGCTGTTCA ACCCATTGGA GCAGGAGCGG
    CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GTGTGCTTTC GGAGGAGGGT
    CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC
    GTCTATCAGG TATCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG
    CAGCAGGGCC TGGACGGGCC CCGCACGCTG CCCTCCTCCG TGCGCGTCTA CCTACACGGG
    CGGCCGCTGT CTGTCAGCAG GAATGAGGCA TTCCCTCTCC GTGTCATTGA CTCGGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGTG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC
    GTCTATGGCA CCCGCTCGTC CAAAGATAAG AGTGGAGCCT ACCTCTTTCT GCCTGACGGC
    GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC
    TTCTCAGAGG TGGTTGCCTG CTACGAGCAC GTTCACCAGG TGGTCCGGCT GTATAACCTG
    CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG CGACTACGTC
    AACAAGGAGC TGGCCCTGCG CATTCGCACG GACATCAACA GCCAGGGCAT CTTCTTCACG
    GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC
    AACTTCTACC CCATGCCTGT CATGGCCTAC CTCCAGGACG CCCAGAACCG CCTCACGCTG
    CACACGGCCC AGGCCCTGGG CGTCTCCAGC CTCCACGATG GCCAGCTAGA GGTGATCTTG
    GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTCGGCC AAGGGCTCAA GGACAACAAG
    AGAACCTGCA ACCACTTCCG CCTCCTGTTA GAACGGCGAA CCCCAGGCAG GGAGCCTGGC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG
    AACCGAGAGG TAAGGGCCAG TGATGCGGTG TCACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.