Man2a2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Man2a2
Entrez Gene ID	308757
Full Name	mannosidase, alpha, class 2A, member 2
General protein information	Preferred Names mannosidase, alpha, class 2A, member 2 Names alpha-mannosidase 2x mannosidase 2, alpha 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q31

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107527.1	NP_001100997.1	alpha-mannosidase 2x
XM_017589165.1	XP_017444654.1	alpha-mannosidase 2x isoform X1
XM_008759529.2	XP_008757751.1	alpha-mannosidase 2x isoform X1
XM_008759527.2	XP_008757749.1	alpha-mannosidase 2x isoform X1
XM_008759530.2	XP_008757752.1	alpha-mannosidase 2x isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Man2a2	NP_001100997.1
Oryza sativa (rice)	Os06g0245700	NP_001057277.2
Xenopus tropicalis (tropical clawed frog)	man2a2	NP_001006777.1
Pan troglodytes (chimpanzee)	MAN2A2	XP_001169951.1
Gallus gallus (chicken)	MAN2A2	XP_001233608.2
Danio rerio (zebrafish)	man2a2	XP_005163040.1
Homo sapiens (human)	MAN2A2	NP_006113.2
Bos taurus (cattle)	MAN2A2	NP_001179570.1
Mus musculus (house mouse)	Man2a2	NP_766491.2
Drosophila melanogaster (fruit fly)	alpha-Man-II	NP_524291.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004020	XP_318477.4
Canis lupus familiaris (dog)	MAN2A2	XP_545863.3
Arabidopsis thaliana (thale cress)	GMII	NP_196999.1
Caenorhabditis elegans (roundworm)	aman-2	NP_505995.2

Gene Ontology
Bibliography

Related articles in PubMed

Essential and mutually compensatory roles of {alpha}-mannosidase II and {alpha}-mannosidase IIx in N-glycan processing in vivo in mice.
Akama TO, Nakagawa H, Wong NK, Sutton-Smith M, Dell A, Morris HR, Nakayama J, Nishimura S, Pai A, Moremen KW, Marth JD, Fukuda MN
Proceedings of the National Academy of Sciences of the United States of America103(24)8983-8(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Man2a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Man2a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11305	NM_001107527.1 Latest version!	Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa46658	XM_008759527.2 Latest version!	Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa46658	XM_008759529.2 Latest version!	Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa46658	XM_017589165.1 Latest version!	Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa46659	XM_008759530.2 Latest version!	Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Man2a2 ( NM_001107527.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100997.1 Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), mRNA.

Price & Availability↑

CloneID	ORa11305
Clone ID Related Accession (Same CDS sequence)	NM_001107527.1
Accession Version	NM_001107527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	alpha-mannosidase 2x
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473980.1. On or before Oct 4, 2007 this sequence version replaced XM_218816.4, XM_001066272.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGC GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCCCTGTACC TAATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT TTCTGTGCTA CAGAACCGGA TCGAACAGCT GGAACAGCTG 
CTGGAAGAAA ACCATGAGAT CATAAGCCAT ATCAAGGACT CTGTGCTGGA ACTGACAGCC 
AATGCGGAGG GCCCACCAGC CCTGCTGCCC TACCACACAG CCAACGGCTC CTGGGCTGTG 
CTCCCCGAGC CCCGGCCCAG CTTCTTCTCT GTATCCCCTG AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAGCTACAG ATGTTAACTG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGCGGCGT GTGGAGGCAA GGCTTCGACA TCTCCTACAG CCCAAATGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCTCACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAAACCCAAC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGATCC CCGACGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAGAAA AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AAATTGCAAC GGGTGGATGG GTGATGCCAG ATGAGGCCAA CTCCCATTAC 
TTTGCCCTGG TCGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACTCCTCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTA ATTCAGAGGG TGCATTACGC CATCAAGAAG 
CACTTTGCGG CCACTCACAG CCTGGAGTTC ATGTGGAGGC AGACATGGGA TTCAGACTCC 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ACGACGTCCC ACACACCTGT 
GGCCCTGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGTCCTT GGAAGGTGCC GCCGCGGGCT ATCACAGAGG CCAACGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TTCGAAGCAG TGTCCTCCTT 
GTGCCGCTGG GTGATGACTT CCGATATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTATCAAC GGCTCTTTGA CTTCCTCAAC AGCAAGCCGG AGTTCCACGT ACAGGCACAG 
TTTGGGACCC TCTCTGAGTA TTTTGATGCC CTGTATAAGA GGACAGGAGT GGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTATGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCC CGGCCTTTCT ATAAGAGCTT GGACCGCGTG 
CTAGAAACTC ACCTTCGTGG GGCAGAGGTT CTATACAGCC TGGCTTTGGC GCATGCCCGC 
CGTTCTGGAC TGACTGGCCA GTATCCGCTG TCTGATTACG CTGTCCTGAC GGAAGCTCGT 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GAACTGCCAA GGAGGCAGTT 
GTAGTAGACT ATGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTAAAGCA GGTCATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC AAGGAGACCT ACAGCTTTGA CCCTAGGGCA 
CCCTTCCTCC AAATGGATGA CAGTCGAGTA AGTCACGATG CCCTGCCTGA GCGCACGGTG 
ATCCGTCTGG ACTCCTCACC CAGGTTTGTG GTGGTCTTTA ACCCACTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCA CCCCGAGTGC GAGTGCTTTC AGAGGAGGGT 
CAGCCCTTGT CTGTGCAGAT CAGTGTGCAG TGGAGCTCCG CCACCAACAT GGTCCCCGAT 
GTCTACCAGG TGTCAGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGACC CTACACACTG CAGTCTTCGG TGCATGTCTA CCTGAACGGC 
GTGAAACTGT CTGTCAGCAG GCAAACAACA TTCCCTCTCC GTGTTGTGGA CTCGGGCACC 
AGTGACTTCG CCATCAGCAA TCGATACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCG TCCGACGTGT GGACGAAGAG CAGGAACAGC AGGTGGACAT GAAGCTCTTC 
GTCTATGGAA CCCGCACATC CAAGGATAAG AGTGGTGCCT ACCTCTTCCT GCCTGATAAC 
GAGGCTAAGC CCTATGTCCC TAAGAAACCT CCTGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGCGTA TTATGAGCAC TTTCACCAAG TGATTCGACT TTACAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACGTG TCGTTCCAGG TGGACATCAG GGACTACGTG 
AACAAGGAGC TAGCCCTGCG CATCCACACA GACATCGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGGT ACAGCCCCGG AAGTATCTGA AGAAGTTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAC ATCCAGGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCTC AGGCTCTGGG TGTCTCCAGC CTCGGCAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCATTTCCG CCTCCTGTTA GAACGTCGAA CCCTGATGAG CCCTGAGGTC 
CAACAGGAGC GCTCTACAAG CTACCCGTCC CTCCTCAGCC ACATGACTTC CATGTACCTC 
AACACACCTC CTCTGGTCTT ACCGGTGGCC AAGAGGGAGA GCACCAGCCC CACTCTGCAC 
TCTTTCCACC CTCTGGCTTC TCCGTTGCCC TGCGATTTCC ATCTGCTCAA TCTGCGCATG 
CTCCCCGCCG AGGTGAGTGT CCCGGTCCGT GCCAATCCTC ACCATCAGGC TGAGGACACC 
TTGCCCGCGG CTGATGCTGC TCTCATCCTA CACCGCAAGG GTTTTGACTG TGGCCTTGAA 
GCCAAGAACC TGGGCTTCAA CTGTACCACA AGCCAAGGCA AGCTGGCCCT GGGGAGCCTC 
TTCCATGGCC TGGATGTGCT ATTCCTGCAG CCCACCTCTT TGACTTTGCT ATACCCTCTG 
GCCTCGCCCT CCAACAGCAC TGACATCTCT CTGGAGCCCA TGGAGATCAG CACCTTCCGC 
CTGCGCTTGG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100997.1
CDS	306..3800
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPALLPYHTANGSWAVLPEPRPSFFSVSPEDCQFALGGRGQKPELQMLTVSEDLPFDNVEGGVWRQGFDISYSPNDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALVDQLIEGHQWLERNLGATPRSGWAVDPFGHSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVADRAALLLDQYRKKSRLFRSSVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPEFHVQAQFGTLSEYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLETHLRGAEVLYSLALAHARRSGLTGQYPLSDYAVLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIINAAHYLVLGDKETYSFDPRAPFLQMDDSRVSHDALPERTVIRLDSSPRFVVVFNPLEQERLSVVSLLVNSPRVRVLSEEGQPLSVQISVQWSSATNMVPDVYQVSVPVRLPALGLGVLQLQPDLDGPYTLQSSVHVYLNGVKLSVSRQTTFPLRVVDSGTSDFAISNRYMQVWFSGLTGLLKSVRRVDEEQEQQVDMKLFVYGTRTSKDKSGAYLFLPDNEAKPYVPKKPPVLRVTEGPFFSEVAAYYEHFHQVIRLYNLPGVEGLSLDVSFQVDIRDYVNKELALRIHTDIDSQGTFFTDLNGFQVQPRKYLKKLPLQANFYPMPVMAYIQDSQRRLTLHTAQALGVSSLGNGQLEVILDRRLMQDDNRGLGQGLKDNKITCNHFRLLLERRTLMSPEVQQERSTSYPSLLSHMTSMYLNTPPLVLPVAKRESTSPTLHSFHPLASPLPCDFHLLNLRMLPAEVSVPVRANPHHQAEDTLPAADAALILHRKGFDCGLEAKNLGFNCTTSQGKLALGSLFHGLDVLFLQPTSLTLLYPLASPSNSTDISLEPMEISTFRLRLG

Target ORF information:

RefSeq Version	NM_001107527.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107527.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGC GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCCCTGTACC TAATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT TTCTGTGCTA CAGAACCGGA TCGAACAGCT GGAACAGCTG 
CTGGAAGAAA ACCATGAGAT CATAAGCCAT ATCAAGGACT CTGTGCTGGA ACTGACAGCC 
AATGCGGAGG GCCCACCAGC CCTGCTGCCC TACCACACAG CCAACGGCTC CTGGGCTGTG 
CTCCCCGAGC CCCGGCCCAG CTTCTTCTCT GTATCCCCTG AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAGCTACAG ATGTTAACTG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGCGGCGT GTGGAGGCAA GGCTTCGACA TCTCCTACAG CCCAAATGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCTCACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAAACCCAAC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGATCC CCGACGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAGAAA AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AAATTGCAAC GGGTGGATGG GTGATGCCAG ATGAGGCCAA CTCCCATTAC 
TTTGCCCTGG TCGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACTCCTCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTA ATTCAGAGGG TGCATTACGC CATCAAGAAG 
CACTTTGCGG CCACTCACAG CCTGGAGTTC ATGTGGAGGC AGACATGGGA TTCAGACTCC 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ACGACGTCCC ACACACCTGT 
GGCCCTGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGTCCTT GGAAGGTGCC GCCGCGGGCT ATCACAGAGG CCAACGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TTCGAAGCAG TGTCCTCCTT 
GTGCCGCTGG GTGATGACTT CCGATATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTATCAAC GGCTCTTTGA CTTCCTCAAC AGCAAGCCGG AGTTCCACGT ACAGGCACAG 
TTTGGGACCC TCTCTGAGTA TTTTGATGCC CTGTATAAGA GGACAGGAGT GGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTATGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCC CGGCCTTTCT ATAAGAGCTT GGACCGCGTG 
CTAGAAACTC ACCTTCGTGG GGCAGAGGTT CTATACAGCC TGGCTTTGGC GCATGCCCGC 
CGTTCTGGAC TGACTGGCCA GTATCCGCTG TCTGATTACG CTGTCCTGAC GGAAGCTCGT 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GAACTGCCAA GGAGGCAGTT 
GTAGTAGACT ATGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTAAAGCA GGTCATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC AAGGAGACCT ACAGCTTTGA CCCTAGGGCA 
CCCTTCCTCC AAATGGATGA CAGTCGAGTA AGTCACGATG CCCTGCCTGA GCGCACGGTG 
ATCCGTCTGG ACTCCTCACC CAGGTTTGTG GTGGTCTTTA ACCCACTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCA CCCCGAGTGC GAGTGCTTTC AGAGGAGGGT 
CAGCCCTTGT CTGTGCAGAT CAGTGTGCAG TGGAGCTCCG CCACCAACAT GGTCCCCGAT 
GTCTACCAGG TGTCAGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGACC CTACACACTG CAGTCTTCGG TGCATGTCTA CCTGAACGGC 
GTGAAACTGT CTGTCAGCAG GCAAACAACA TTCCCTCTCC GTGTTGTGGA CTCGGGCACC 
AGTGACTTCG CCATCAGCAA TCGATACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCG TCCGACGTGT GGACGAAGAG CAGGAACAGC AGGTGGACAT GAAGCTCTTC 
GTCTATGGAA CCCGCACATC CAAGGATAAG AGTGGTGCCT ACCTCTTCCT GCCTGATAAC 
GAGGCTAAGC CCTATGTCCC TAAGAAACCT CCTGTGCTGC GTGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGCGTA TTATGAGCAC TTTCACCAAG TGATTCGACT TTACAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACGTG TCGTTCCAGG TGGACATCAG GGACTACGTG 
AACAAGGAGC TAGCCCTGCG CATCCACACA GACATCGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGGT ACAGCCCCGG AAGTATCTGA AGAAGTTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAC ATCCAGGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCTC AGGCTCTGGG TGTCTCCAGC CTCGGCAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCATTTCCG CCTCCTGTTA GAACGTCGAA CCCTGATGAG CCCTGAGGTC 
CAACAGGAGC GCTCTACAAG CTACCCGTCC CTCCTCAGCC ACATGACTTC CATGTACCTC 
AACACACCTC CTCTGGTCTT ACCGGTGGCC AAGAGGGAGA GCACCAGCCC CACTCTGCAC 
TCTTTCCACC CTCTGGCTTC TCCGTTGCCC TGCGATTTCC ATCTGCTCAA TCTGCGCATG 
CTCCCCGCCG AGGTGAGTGT CCCGGTCCGT GCCAATCCTC ACCATCAGGC TGAGGACACC 
TTGCCCGCGG CTGATGCTGC TCTCATCCTA CACCGCAAGG GTTTTGACTG TGGCCTTGAA 
GCCAAGAACC TGGGCTTCAA CTGTACCACA AGCCAAGGCA AGCTGGCCCT GGGGAGCCTC 
TTCCATGGCC TGGATGTGCT ATTCCTGCAG CCCACCTCTT TGACTTTGCT ATACCCTCTG 
GCCTCGCCCT CCAACAGCAC TGACATCTCT CTGGAGCCCA TGGAGATCAG CACCTTCCGC 
CTGCGCTTGG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2a2 ( XM_017589165.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444654.1 Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa46658
Clone ID Related Accession (Same CDS sequence)	XM_017589165.1 , XM_008759529.2 , XM_008759527.2
Accession Version	XM_017589165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGC GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCCCTGTACC TAATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT TTCTGTGCTA CAGAACCGGA TCGAACAGCT GGAACAGCTG 
CTGGAAGAAA ACCATGAGAT CATAAGCCAT ATCAAGGACT CTGTGCTGGA ACTGACAGCC 
AATGCGGAGG GCCCACCAGC CCTGCTGCCC TACCACACAG CCAACGGCTC CTGGGCTGTG 
CTCCCCGAGC CCCGGCCCAG CTTCTTCTCT GTATCCCCTG AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAGCTACAG ATGTTAACTG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGCGGCGT GTGGAGGCAA GGCTTCGACA TCTCCTACAG CCCAAATGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCTCACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAAACCCAAC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGATCC CCGACGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAGAAA AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AAATTGCAAC GGGTGGATGG GTGATGCCAG ATGAGGCCAA CTCCCATTAC 
TTTGCCCTGG TCGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACTCCTCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTA ATTCAGAGGG TGCATTACGC CATCAAGAAG 
CACTTTGCGG CCACTCACAG CCTGGAGTTC ATGTGGAGGC AGACATGGGA TTCAGACTCC 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ACGACGTCCC ACACACCTGT 
GGCCCTGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGTCCTT GGAAGGTGCC GCCGCGGGCT ATCACAGAGG CCAACGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TTCGAAGCAG TGTCCTCCTT 
GTGCCGCTGG GTGATGACTT CCGATATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTATCAAC GGCTCTTTGA CTTCCTCAAC AGCAAGCCGG AGTTCCACGT ACAGGCACAG 
TTTGGGACCC TCTCTGAGTA TTTTGATGCC CTGTATAAGA GGACAGGAGT GGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTATGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCC CGGCCTTTCT ATAAGAGCTT GGACCGCGTG 
CTAGAAACTC ACCTTCGTGG GGCAGAGGTT CTATACAGCC TGGCTTTGGC GCATGCCCGC 
CGTTCTGGAC TGACTGGCCA GTATCCGCTG TCTGATTACG CTGTCCTGAC GGAAGCTCGT 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GAACTGCCAA GGAGGCAGTT 
GTAGTAGACT ATGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTAAAGCA GGTCATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC AAGGAGACCT ACAGCTTTGA CCCTAGGGCA 
CCCTTCCTCC AAATGGATGA CAGTCGAGTA AGTCACGATG CCCTGCCTGA GCGCACGGTG 
ATCCGTCTGG ACTCCTCACC CAGGTTTGTG GTGGTCTTTA ACCCACTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCA CCCCGAGTGC GAGTGCTTTC AGAGGAGGGT 
CAGCCCTTGT CTGTGCAGAT CAGTGTGCAG TGGAGCTCCG CCACCAACAT GGTCCCCGAT 
GTCTACCAGG TGTCAGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGACC CTACACACTG CAGTCTTCGG TGCATGTCTA CCTGAACGGC 
GTGAAACTGT CTGTCAGCAG GCAAACAACA TTCCCTCTCC GTGTTGTGGA CTCGGGCACC 
AGTGACTTCG CCATCAGCAA TCGATACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCG TCCGACGTGT GGACGAAGAG CAGGAACAGC AGGTGGACAT GAAGCTCTTC 
GTCTATGGAA CCCGCACATC CAAGGATAAG AGTGGTGCCT ACCTCTTCCT GCCTGATAAC 
GAGGCTAAGC CCTATGTCCC TAAGAAACCT CCTGTGCTGC GCGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGCGTA TTATGAGCAC TTTCACCAAG TGATTCGACT TTACAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACGTG TCGTTCCAGG TGGACATCAG GGACTACGTG 
AACAAGGAGC TAGCCCTGCG CATCCACACA GACATCGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGGT ACAGCCCCGG AAGTATCTGA AGAAGTTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAC ATCCAGGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCTC AGGCTCTGGG TGTCTCCAGC CTCGGCAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCATTTCCG CCTCCTGTTA GAACGTCGAA CCCTGATGAG CCCTGAGCCT 
GGCTTTTTCT CCAAACTGGC AGCCATGTTT AGGGGCTTGA TCTTTTCCAG CAACACAATC 
AGGAACTCAA AGGTCCAACA GGAGCGCTCT ACAAGCTACC CGTCCCTCCT CAGCCACATG 
ACTTCCATGT ACCTCAACAC ACCTCCTCTG GTCTTACCGG TGGCCAAGAG GGAGAGCACC 
AGCCCCACTC TGCACTCTTT CCACCCTCTG GCTTCTCCGT TGCCCTGCGA TTTCCATCTG 
CTCAATCTGC GCATGCTCCC CGCCGAGGAC ACCTTGCCCG CGGCTGATGC TGCTCTCATC 
CTACACCGCA AGGGTTTTGA CTGTGGCCTT GAAGCCAAGA ACCTGGGCTT CAACTGTACC 
ACAAGCCAAG GCAAGCTGGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT GCTATTCCTG 
CAGCCCACCT CTTTGACTTT GCTATACCCT CTGGCCTCGC CCTCCAACAG CACTGACATC 
TCTCTGGAGC CCATGGAGAT CAGCACCTTC CGCCTGCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444654.1
CDS	60..3587
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPALLPYHTANGSWAVLPEPRPSFFSVSPEDCQFALGGRGQKPELQMLTVSEDLPFDNVEGGVWRQGFDISYSPNDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALVDQLIEGHQWLERNLGATPRSGWAVDPFGHSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVADRAALLLDQYRKKSRLFRSSVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPEFHVQAQFGTLSEYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLETHLRGAEVLYSLALAHARRSGLTGQYPLSDYAVLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIINAAHYLVLGDKETYSFDPRAPFLQMDDSRVSHDALPERTVIRLDSSPRFVVVFNPLEQERLSVVSLLVNSPRVRVLSEEGQPLSVQISVQWSSATNMVPDVYQVSVPVRLPALGLGVLQLQPDLDGPYTLQSSVHVYLNGVKLSVSRQTTFPLRVVDSGTSDFAISNRYMQVWFSGLTGLLKSVRRVDEEQEQQVDMKLFVYGTRTSKDKSGAYLFLPDNEAKPYVPKKPPVLRVTEGPFFSEVAAYYEHFHQVIRLYNLPGVEGLSLDVSFQVDIRDYVNKELALRIHTDIDSQGTFFTDLNGFQVQPRKYLKKLPLQANFYPMPVMAYIQDSQRRLTLHTAQALGVSSLGNGQLEVILDRRLMQDDNRGLGQGLKDNKITCNHFRLLLERRTLMSPEPGFFSKLAAMFRGLIFSSNTIRNSKVQQERSTSYPSLLSHMTSMYLNTPPLVLPVAKRESTSPTLHSFHPLASPLPCDFHLLNLRMLPAEDTLPAADAALILHRKGFDCGLEAKNLGFNCTTSQGKLALGSLFHGLDVLFLQPTSLTLLYPLASPSNSTDISLEPMEISTFRLRLG

Target ORF information:

RefSeq Version	XM_017589165.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589165.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGC GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCCCTGTACC TAATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT TTCTGTGCTA CAGAACCGGA TCGAACAGCT GGAACAGCTG 
CTGGAAGAAA ACCATGAGAT CATAAGCCAT ATCAAGGACT CTGTGCTGGA ACTGACAGCC 
AATGCGGAGG GCCCACCAGC CCTGCTGCCC TACCACACAG CCAACGGCTC CTGGGCTGTG 
CTCCCCGAGC CCCGGCCCAG CTTCTTCTCT GTATCCCCTG AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAGCTACAG ATGTTAACTG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGCGGCGT GTGGAGGCAA GGCTTCGACA TCTCCTACAG CCCAAATGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCTCACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAAACCCAAC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGATCC CCGACGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAGAAA AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AAATTGCAAC GGGTGGATGG GTGATGCCAG ATGAGGCCAA CTCCCATTAC 
TTTGCCCTGG TCGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACTCCTCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTA ATTCAGAGGG TGCATTACGC CATCAAGAAG 
CACTTTGCGG CCACTCACAG CCTGGAGTTC ATGTGGAGGC AGACATGGGA TTCAGACTCC 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ACGACGTCCC ACACACCTGT 
GGCCCTGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGTCCTT GGAAGGTGCC GCCGCGGGCT ATCACAGAGG CCAACGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TTCGAAGCAG TGTCCTCCTT 
GTGCCGCTGG GTGATGACTT CCGATATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTATCAAC GGCTCTTTGA CTTCCTCAAC AGCAAGCCGG AGTTCCACGT ACAGGCACAG 
TTTGGGACCC TCTCTGAGTA TTTTGATGCC CTGTATAAGA GGACAGGAGT GGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTATGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCC CGGCCTTTCT ATAAGAGCTT GGACCGCGTG 
CTAGAAACTC ACCTTCGTGG GGCAGAGGTT CTATACAGCC TGGCTTTGGC GCATGCCCGC 
CGTTCTGGAC TGACTGGCCA GTATCCGCTG TCTGATTACG CTGTCCTGAC GGAAGCTCGT 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GAACTGCCAA GGAGGCAGTT 
GTAGTAGACT ATGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTAAAGCA GGTCATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC AAGGAGACCT ACAGCTTTGA CCCTAGGGCA 
CCCTTCCTCC AAATGGATGA CAGTCGAGTA AGTCACGATG CCCTGCCTGA GCGCACGGTG 
ATCCGTCTGG ACTCCTCACC CAGGTTTGTG GTGGTCTTTA ACCCACTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCA CCCCGAGTGC GAGTGCTTTC AGAGGAGGGT 
CAGCCCTTGT CTGTGCAGAT CAGTGTGCAG TGGAGCTCCG CCACCAACAT GGTCCCCGAT 
GTCTACCAGG TGTCAGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGACC CTACACACTG CAGTCTTCGG TGCATGTCTA CCTGAACGGC 
GTGAAACTGT CTGTCAGCAG GCAAACAACA TTCCCTCTCC GTGTTGTGGA CTCGGGCACC 
AGTGACTTCG CCATCAGCAA TCGATACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCG TCCGACGTGT GGACGAAGAG CAGGAACAGC AGGTGGACAT GAAGCTCTTC 
GTCTATGGAA CCCGCACATC CAAGGATAAG AGTGGTGCCT ACCTCTTCCT GCCTGATAAC 
GAGGCTAAGC CCTATGTCCC TAAGAAACCT CCTGTGCTGC GCGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGCGTA TTATGAGCAC TTTCACCAAG TGATTCGACT TTACAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACGTG TCGTTCCAGG TGGACATCAG GGACTACGTG 
AACAAGGAGC TAGCCCTGCG CATCCACACA GACATCGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGGT ACAGCCCCGG AAGTATCTGA AGAAGTTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAC ATCCAGGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCTC AGGCTCTGGG TGTCTCCAGC CTCGGCAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCATTTCCG CCTCCTGTTA GAACGTCGAA CCCTGATGAG CCCTGAGCCT 
GGCTTTTTCT CCAAACTGGC AGCCATGTTT AGGGGCTTGA TCTTTTCCAG CAACACAATC 
AGGAACTCAA AGGTCCAACA GGAGCGCTCT ACAAGCTACC CGTCCCTCCT CAGCCACATG 
ACTTCCATGT ACCTCAACAC ACCTCCTCTG GTCTTACCGG TGGCCAAGAG GGAGAGCACC 
AGCCCCACTC TGCACTCTTT CCACCCTCTG GCTTCTCCGT TGCCCTGCGA TTTCCATCTG 
CTCAATCTGC GCATGCTCCC CGCCGAGGAC ACCTTGCCCG CGGCTGATGC TGCTCTCATC 
CTACACCGCA AGGGTTTTGA CTGTGGCCTT GAAGCCAAGA ACCTGGGCTT CAACTGTACC 
ACAAGCCAAG GCAAGCTGGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT GCTATTCCTG 
CAGCCCACCT CTTTGACTTT GCTATACCCT CTGGCCTCGC CCTCCAACAG CACTGACATC 
TCTCTGGAGC CCATGGAGAT CAGCACCTTC CGCCTGCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2a2 ( XM_008759529.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757751.1 Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), transcript variant X2, mRNA.

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CloneID	ORa46658
Clone ID Related Accession (Same CDS sequence)	XM_017589165.1 , XM_008759529.2 , XM_008759527.2
Accession Version	XM_008759529.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGC GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCCCTGTACC TAATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT TTCTGTGCTA CAGAACCGGA TCGAACAGCT GGAACAGCTG 
CTGGAAGAAA ACCATGAGAT CATAAGCCAT ATCAAGGACT CTGTGCTGGA ACTGACAGCC 
AATGCGGAGG GCCCACCAGC CCTGCTGCCC TACCACACAG CCAACGGCTC CTGGGCTGTG 
CTCCCCGAGC CCCGGCCCAG CTTCTTCTCT GTATCCCCTG AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAGCTACAG ATGTTAACTG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGCGGCGT GTGGAGGCAA GGCTTCGACA TCTCCTACAG CCCAAATGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCTCACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAAACCCAAC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGATCC CCGACGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAGAAA AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AAATTGCAAC GGGTGGATGG GTGATGCCAG ATGAGGCCAA CTCCCATTAC 
TTTGCCCTGG TCGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACTCCTCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTA ATTCAGAGGG TGCATTACGC CATCAAGAAG 
CACTTTGCGG CCACTCACAG CCTGGAGTTC ATGTGGAGGC AGACATGGGA TTCAGACTCC 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ACGACGTCCC ACACACCTGT 
GGCCCTGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGTCCTT GGAAGGTGCC GCCGCGGGCT ATCACAGAGG CCAACGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TTCGAAGCAG TGTCCTCCTT 
GTGCCGCTGG GTGATGACTT CCGATATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTATCAAC GGCTCTTTGA CTTCCTCAAC AGCAAGCCGG AGTTCCACGT ACAGGCACAG 
TTTGGGACCC TCTCTGAGTA TTTTGATGCC CTGTATAAGA GGACAGGAGT GGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTATGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCC CGGCCTTTCT ATAAGAGCTT GGACCGCGTG 
CTAGAAACTC ACCTTCGTGG GGCAGAGGTT CTATACAGCC TGGCTTTGGC GCATGCCCGC 
CGTTCTGGAC TGACTGGCCA GTATCCGCTG TCTGATTACG CTGTCCTGAC GGAAGCTCGT 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GAACTGCCAA GGAGGCAGTT 
GTAGTAGACT ATGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTAAAGCA GGTCATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC AAGGAGACCT ACAGCTTTGA CCCTAGGGCA 
CCCTTCCTCC AAATGGATGA CAGTCGAGTA AGTCACGATG CCCTGCCTGA GCGCACGGTG 
ATCCGTCTGG ACTCCTCACC CAGGTTTGTG GTGGTCTTTA ACCCACTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCA CCCCGAGTGC GAGTGCTTTC AGAGGAGGGT 
CAGCCCTTGT CTGTGCAGAT CAGTGTGCAG TGGAGCTCCG CCACCAACAT GGTCCCCGAT 
GTCTACCAGG TGTCAGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGACC CTACACACTG CAGTCTTCGG TGCATGTCTA CCTGAACGGC 
GTGAAACTGT CTGTCAGCAG GCAAACAACA TTCCCTCTCC GTGTTGTGGA CTCGGGCACC 
AGTGACTTCG CCATCAGCAA TCGATACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCG TCCGACGTGT GGACGAAGAG CAGGAACAGC AGGTGGACAT GAAGCTCTTC 
GTCTATGGAA CCCGCACATC CAAGGATAAG AGTGGTGCCT ACCTCTTCCT GCCTGATAAC 
GAGGCTAAGC CCTATGTCCC TAAGAAACCT CCTGTGCTGC GCGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGCGTA TTATGAGCAC TTTCACCAAG TGATTCGACT TTACAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACGTG TCGTTCCAGG TGGACATCAG GGACTACGTG 
AACAAGGAGC TAGCCCTGCG CATCCACACA GACATCGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGGT ACAGCCCCGG AAGTATCTGA AGAAGTTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAC ATCCAGGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCTC AGGCTCTGGG TGTCTCCAGC CTCGGCAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCATTTCCG CCTCCTGTTA GAACGTCGAA CCCTGATGAG CCCTGAGCCT 
GGCTTTTTCT CCAAACTGGC AGCCATGTTT AGGGGCTTGA TCTTTTCCAG CAACACAATC 
AGGAACTCAA AGGTCCAACA GGAGCGCTCT ACAAGCTACC CGTCCCTCCT CAGCCACATG 
ACTTCCATGT ACCTCAACAC ACCTCCTCTG GTCTTACCGG TGGCCAAGAG GGAGAGCACC 
AGCCCCACTC TGCACTCTTT CCACCCTCTG GCTTCTCCGT TGCCCTGCGA TTTCCATCTG 
CTCAATCTGC GCATGCTCCC CGCCGAGGAC ACCTTGCCCG CGGCTGATGC TGCTCTCATC 
CTACACCGCA AGGGTTTTGA CTGTGGCCTT GAAGCCAAGA ACCTGGGCTT CAACTGTACC 
ACAAGCCAAG GCAAGCTGGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT GCTATTCCTG 
CAGCCCACCT CTTTGACTTT GCTATACCCT CTGGCCTCGC CCTCCAACAG CACTGACATC 
TCTCTGGAGC CCATGGAGAT CAGCACCTTC CGCCTGCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757751.1
CDS	595..4122
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPALLPYHTANGSWAVLPEPRPSFFSVSPEDCQFALGGRGQKPELQMLTVSEDLPFDNVEGGVWRQGFDISYSPNDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALVDQLIEGHQWLERNLGATPRSGWAVDPFGHSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVADRAALLLDQYRKKSRLFRSSVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPEFHVQAQFGTLSEYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLETHLRGAEVLYSLALAHARRSGLTGQYPLSDYAVLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIINAAHYLVLGDKETYSFDPRAPFLQMDDSRVSHDALPERTVIRLDSSPRFVVVFNPLEQERLSVVSLLVNSPRVRVLSEEGQPLSVQISVQWSSATNMVPDVYQVSVPVRLPALGLGVLQLQPDLDGPYTLQSSVHVYLNGVKLSVSRQTTFPLRVVDSGTSDFAISNRYMQVWFSGLTGLLKSVRRVDEEQEQQVDMKLFVYGTRTSKDKSGAYLFLPDNEAKPYVPKKPPVLRVTEGPFFSEVAAYYEHFHQVIRLYNLPGVEGLSLDVSFQVDIRDYVNKELALRIHTDIDSQGTFFTDLNGFQVQPRKYLKKLPLQANFYPMPVMAYIQDSQRRLTLHTAQALGVSSLGNGQLEVILDRRLMQDDNRGLGQGLKDNKITCNHFRLLLERRTLMSPEPGFFSKLAAMFRGLIFSSNTIRNSKVQQERSTSYPSLLSHMTSMYLNTPPLVLPVAKRESTSPTLHSFHPLASPLPCDFHLLNLRMLPAEDTLPAADAALILHRKGFDCGLEAKNLGFNCTTSQGKLALGSLFHGLDVLFLQPTSLTLLYPLASPSNSTDISLEPMEISTFRLRLG

Target ORF information:

RefSeq Version	XM_008759529.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759529.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGC GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCCCTGTACC TAATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT TTCTGTGCTA CAGAACCGGA TCGAACAGCT GGAACAGCTG 
CTGGAAGAAA ACCATGAGAT CATAAGCCAT ATCAAGGACT CTGTGCTGGA ACTGACAGCC 
AATGCGGAGG GCCCACCAGC CCTGCTGCCC TACCACACAG CCAACGGCTC CTGGGCTGTG 
CTCCCCGAGC CCCGGCCCAG CTTCTTCTCT GTATCCCCTG AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAGCTACAG ATGTTAACTG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGCGGCGT GTGGAGGCAA GGCTTCGACA TCTCCTACAG CCCAAATGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCTCACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAAACCCAAC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGATCC CCGACGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAGAAA AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AAATTGCAAC GGGTGGATGG GTGATGCCAG ATGAGGCCAA CTCCCATTAC 
TTTGCCCTGG TCGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACTCCTCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTA ATTCAGAGGG TGCATTACGC CATCAAGAAG 
CACTTTGCGG CCACTCACAG CCTGGAGTTC ATGTGGAGGC AGACATGGGA TTCAGACTCC 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ACGACGTCCC ACACACCTGT 
GGCCCTGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGTCCTT GGAAGGTGCC GCCGCGGGCT ATCACAGAGG CCAACGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TTCGAAGCAG TGTCCTCCTT 
GTGCCGCTGG GTGATGACTT CCGATATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTATCAAC GGCTCTTTGA CTTCCTCAAC AGCAAGCCGG AGTTCCACGT ACAGGCACAG 
TTTGGGACCC TCTCTGAGTA TTTTGATGCC CTGTATAAGA GGACAGGAGT GGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTATGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCC CGGCCTTTCT ATAAGAGCTT GGACCGCGTG 
CTAGAAACTC ACCTTCGTGG GGCAGAGGTT CTATACAGCC TGGCTTTGGC GCATGCCCGC 
CGTTCTGGAC TGACTGGCCA GTATCCGCTG TCTGATTACG CTGTCCTGAC GGAAGCTCGT 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GAACTGCCAA GGAGGCAGTT 
GTAGTAGACT ATGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTAAAGCA GGTCATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC AAGGAGACCT ACAGCTTTGA CCCTAGGGCA 
CCCTTCCTCC AAATGGATGA CAGTCGAGTA AGTCACGATG CCCTGCCTGA GCGCACGGTG 
ATCCGTCTGG ACTCCTCACC CAGGTTTGTG GTGGTCTTTA ACCCACTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCA CCCCGAGTGC GAGTGCTTTC AGAGGAGGGT 
CAGCCCTTGT CTGTGCAGAT CAGTGTGCAG TGGAGCTCCG CCACCAACAT GGTCCCCGAT 
GTCTACCAGG TGTCAGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGACC CTACACACTG CAGTCTTCGG TGCATGTCTA CCTGAACGGC 
GTGAAACTGT CTGTCAGCAG GCAAACAACA TTCCCTCTCC GTGTTGTGGA CTCGGGCACC 
AGTGACTTCG CCATCAGCAA TCGATACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCG TCCGACGTGT GGACGAAGAG CAGGAACAGC AGGTGGACAT GAAGCTCTTC 
GTCTATGGAA CCCGCACATC CAAGGATAAG AGTGGTGCCT ACCTCTTCCT GCCTGATAAC 
GAGGCTAAGC CCTATGTCCC TAAGAAACCT CCTGTGCTGC GCGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGCGTA TTATGAGCAC TTTCACCAAG TGATTCGACT TTACAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACGTG TCGTTCCAGG TGGACATCAG GGACTACGTG 
AACAAGGAGC TAGCCCTGCG CATCCACACA GACATCGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGGT ACAGCCCCGG AAGTATCTGA AGAAGTTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAC ATCCAGGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCTC AGGCTCTGGG TGTCTCCAGC CTCGGCAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCATTTCCG CCTCCTGTTA GAACGTCGAA CCCTGATGAG CCCTGAGCCT 
GGCTTTTTCT CCAAACTGGC AGCCATGTTT AGGGGCTTGA TCTTTTCCAG CAACACAATC 
AGGAACTCAA AGGTCCAACA GGAGCGCTCT ACAAGCTACC CGTCCCTCCT CAGCCACATG 
ACTTCCATGT ACCTCAACAC ACCTCCTCTG GTCTTACCGG TGGCCAAGAG GGAGAGCACC 
AGCCCCACTC TGCACTCTTT CCACCCTCTG GCTTCTCCGT TGCCCTGCGA TTTCCATCTG 
CTCAATCTGC GCATGCTCCC CGCCGAGGAC ACCTTGCCCG CGGCTGATGC TGCTCTCATC 
CTACACCGCA AGGGTTTTGA CTGTGGCCTT GAAGCCAAGA ACCTGGGCTT CAACTGTACC 
ACAAGCCAAG GCAAGCTGGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT GCTATTCCTG 
CAGCCCACCT CTTTGACTTT GCTATACCCT CTGGCCTCGC CCTCCAACAG CACTGACATC 
TCTCTGGAGC CCATGGAGAT CAGCACCTTC CGCCTGCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2a2 ( XM_008759527.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757749.1 Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), transcript variant X1, mRNA.

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CloneID	ORa46658
Clone ID Related Accession (Same CDS sequence)	XM_017589165.1 , XM_008759529.2 , XM_008759527.2
Accession Version	XM_008759527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759527.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGC GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCCCTGTACC TAATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT TTCTGTGCTA CAGAACCGGA TCGAACAGCT GGAACAGCTG 
CTGGAAGAAA ACCATGAGAT CATAAGCCAT ATCAAGGACT CTGTGCTGGA ACTGACAGCC 
AATGCGGAGG GCCCACCAGC CCTGCTGCCC TACCACACAG CCAACGGCTC CTGGGCTGTG 
CTCCCCGAGC CCCGGCCCAG CTTCTTCTCT GTATCCCCTG AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAGCTACAG ATGTTAACTG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGCGGCGT GTGGAGGCAA GGCTTCGACA TCTCCTACAG CCCAAATGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCTCACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAAACCCAAC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGATCC CCGACGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAGAAA AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AAATTGCAAC GGGTGGATGG GTGATGCCAG ATGAGGCCAA CTCCCATTAC 
TTTGCCCTGG TCGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACTCCTCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTA ATTCAGAGGG TGCATTACGC CATCAAGAAG 
CACTTTGCGG CCACTCACAG CCTGGAGTTC ATGTGGAGGC AGACATGGGA TTCAGACTCC 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ACGACGTCCC ACACACCTGT 
GGCCCTGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGTCCTT GGAAGGTGCC GCCGCGGGCT ATCACAGAGG CCAACGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TTCGAAGCAG TGTCCTCCTT 
GTGCCGCTGG GTGATGACTT CCGATATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTATCAAC GGCTCTTTGA CTTCCTCAAC AGCAAGCCGG AGTTCCACGT ACAGGCACAG 
TTTGGGACCC TCTCTGAGTA TTTTGATGCC CTGTATAAGA GGACAGGAGT GGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTATGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCC CGGCCTTTCT ATAAGAGCTT GGACCGCGTG 
CTAGAAACTC ACCTTCGTGG GGCAGAGGTT CTATACAGCC TGGCTTTGGC GCATGCCCGC 
CGTTCTGGAC TGACTGGCCA GTATCCGCTG TCTGATTACG CTGTCCTGAC GGAAGCTCGT 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GAACTGCCAA GGAGGCAGTT 
GTAGTAGACT ATGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTAAAGCA GGTCATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC AAGGAGACCT ACAGCTTTGA CCCTAGGGCA 
CCCTTCCTCC AAATGGATGA CAGTCGAGTA AGTCACGATG CCCTGCCTGA GCGCACGGTG 
ATCCGTCTGG ACTCCTCACC CAGGTTTGTG GTGGTCTTTA ACCCACTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCA CCCCGAGTGC GAGTGCTTTC AGAGGAGGGT 
CAGCCCTTGT CTGTGCAGAT CAGTGTGCAG TGGAGCTCCG CCACCAACAT GGTCCCCGAT 
GTCTACCAGG TGTCAGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGACC CTACACACTG CAGTCTTCGG TGCATGTCTA CCTGAACGGC 
GTGAAACTGT CTGTCAGCAG GCAAACAACA TTCCCTCTCC GTGTTGTGGA CTCGGGCACC 
AGTGACTTCG CCATCAGCAA TCGATACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCG TCCGACGTGT GGACGAAGAG CAGGAACAGC AGGTGGACAT GAAGCTCTTC 
GTCTATGGAA CCCGCACATC CAAGGATAAG AGTGGTGCCT ACCTCTTCCT GCCTGATAAC 
GAGGCTAAGC CCTATGTCCC TAAGAAACCT CCTGTGCTGC GCGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGCGTA TTATGAGCAC TTTCACCAAG TGATTCGACT TTACAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACGTG TCGTTCCAGG TGGACATCAG GGACTACGTG 
AACAAGGAGC TAGCCCTGCG CATCCACACA GACATCGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGGT ACAGCCCCGG AAGTATCTGA AGAAGTTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAC ATCCAGGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCTC AGGCTCTGGG TGTCTCCAGC CTCGGCAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCATTTCCG CCTCCTGTTA GAACGTCGAA CCCTGATGAG CCCTGAGCCT 
GGCTTTTTCT CCAAACTGGC AGCCATGTTT AGGGGCTTGA TCTTTTCCAG CAACACAATC 
AGGAACTCAA AGGTCCAACA GGAGCGCTCT ACAAGCTACC CGTCCCTCCT CAGCCACATG 
ACTTCCATGT ACCTCAACAC ACCTCCTCTG GTCTTACCGG TGGCCAAGAG GGAGAGCACC 
AGCCCCACTC TGCACTCTTT CCACCCTCTG GCTTCTCCGT TGCCCTGCGA TTTCCATCTG 
CTCAATCTGC GCATGCTCCC CGCCGAGGAC ACCTTGCCCG CGGCTGATGC TGCTCTCATC 
CTACACCGCA AGGGTTTTGA CTGTGGCCTT GAAGCCAAGA ACCTGGGCTT CAACTGTACC 
ACAAGCCAAG GCAAGCTGGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT GCTATTCCTG 
CAGCCCACCT CTTTGACTTT GCTATACCCT CTGGCCTCGC CCTCCAACAG CACTGACATC 
TCTCTGGAGC CCATGGAGAT CAGCACCTTC CGCCTGCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757749.1
CDS	600..4127
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPALLPYHTANGSWAVLPEPRPSFFSVSPEDCQFALGGRGQKPELQMLTVSEDLPFDNVEGGVWRQGFDISYSPNDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALVDQLIEGHQWLERNLGATPRSGWAVDPFGHSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVADRAALLLDQYRKKSRLFRSSVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPEFHVQAQFGTLSEYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLETHLRGAEVLYSLALAHARRSGLTGQYPLSDYAVLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIINAAHYLVLGDKETYSFDPRAPFLQMDDSRVSHDALPERTVIRLDSSPRFVVVFNPLEQERLSVVSLLVNSPRVRVLSEEGQPLSVQISVQWSSATNMVPDVYQVSVPVRLPALGLGVLQLQPDLDGPYTLQSSVHVYLNGVKLSVSRQTTFPLRVVDSGTSDFAISNRYMQVWFSGLTGLLKSVRRVDEEQEQQVDMKLFVYGTRTSKDKSGAYLFLPDNEAKPYVPKKPPVLRVTEGPFFSEVAAYYEHFHQVIRLYNLPGVEGLSLDVSFQVDIRDYVNKELALRIHTDIDSQGTFFTDLNGFQVQPRKYLKKLPLQANFYPMPVMAYIQDSQRRLTLHTAQALGVSSLGNGQLEVILDRRLMQDDNRGLGQGLKDNKITCNHFRLLLERRTLMSPEPGFFSKLAAMFRGLIFSSNTIRNSKVQQERSTSYPSLLSHMTSMYLNTPPLVLPVAKRESTSPTLHSFHPLASPLPCDFHLLNLRMLPAEDTLPAADAALILHRKGFDCGLEAKNLGFNCTTSQGKLALGSLFHGLDVLFLQPTSLTLLYPLASPSNSTDISLEPMEISTFRLRLG

Target ORF information:

RefSeq Version	XM_008759527.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759527.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGC GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCCCTGTACC TAATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT TTCTGTGCTA CAGAACCGGA TCGAACAGCT GGAACAGCTG 
CTGGAAGAAA ACCATGAGAT CATAAGCCAT ATCAAGGACT CTGTGCTGGA ACTGACAGCC 
AATGCGGAGG GCCCACCAGC CCTGCTGCCC TACCACACAG CCAACGGCTC CTGGGCTGTG 
CTCCCCGAGC CCCGGCCCAG CTTCTTCTCT GTATCCCCTG AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAGCTACAG ATGTTAACTG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGCGGCGT GTGGAGGCAA GGCTTCGACA TCTCCTACAG CCCAAATGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCTCACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAAACCCAAC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGATCC CCGACGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAGAAA AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AAATTGCAAC GGGTGGATGG GTGATGCCAG ATGAGGCCAA CTCCCATTAC 
TTTGCCCTGG TCGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACTCCTCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTA ATTCAGAGGG TGCATTACGC CATCAAGAAG 
CACTTTGCGG CCACTCACAG CCTGGAGTTC ATGTGGAGGC AGACATGGGA TTCAGACTCC 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ACGACGTCCC ACACACCTGT 
GGCCCTGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGTCCTT GGAAGGTGCC GCCGCGGGCT ATCACAGAGG CCAACGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TTCGAAGCAG TGTCCTCCTT 
GTGCCGCTGG GTGATGACTT CCGATATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTATCAAC GGCTCTTTGA CTTCCTCAAC AGCAAGCCGG AGTTCCACGT ACAGGCACAG 
TTTGGGACCC TCTCTGAGTA TTTTGATGCC CTGTATAAGA GGACAGGAGT GGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTATGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCC CGGCCTTTCT ATAAGAGCTT GGACCGCGTG 
CTAGAAACTC ACCTTCGTGG GGCAGAGGTT CTATACAGCC TGGCTTTGGC GCATGCCCGC 
CGTTCTGGAC TGACTGGCCA GTATCCGCTG TCTGATTACG CTGTCCTGAC GGAAGCTCGT 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GAACTGCCAA GGAGGCAGTT 
GTAGTAGACT ATGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTAAAGCA GGTCATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC AAGGAGACCT ACAGCTTTGA CCCTAGGGCA 
CCCTTCCTCC AAATGGATGA CAGTCGAGTA AGTCACGATG CCCTGCCTGA GCGCACGGTG 
ATCCGTCTGG ACTCCTCACC CAGGTTTGTG GTGGTCTTTA ACCCACTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCA CCCCGAGTGC GAGTGCTTTC AGAGGAGGGT 
CAGCCCTTGT CTGTGCAGAT CAGTGTGCAG TGGAGCTCCG CCACCAACAT GGTCCCCGAT 
GTCTACCAGG TGTCAGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGACC CTACACACTG CAGTCTTCGG TGCATGTCTA CCTGAACGGC 
GTGAAACTGT CTGTCAGCAG GCAAACAACA TTCCCTCTCC GTGTTGTGGA CTCGGGCACC 
AGTGACTTCG CCATCAGCAA TCGATACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCG TCCGACGTGT GGACGAAGAG CAGGAACAGC AGGTGGACAT GAAGCTCTTC 
GTCTATGGAA CCCGCACATC CAAGGATAAG AGTGGTGCCT ACCTCTTCCT GCCTGATAAC 
GAGGCTAAGC CCTATGTCCC TAAGAAACCT CCTGTGCTGC GCGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGCGTA TTATGAGCAC TTTCACCAAG TGATTCGACT TTACAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACGTG TCGTTCCAGG TGGACATCAG GGACTACGTG 
AACAAGGAGC TAGCCCTGCG CATCCACACA GACATCGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGGT ACAGCCCCGG AAGTATCTGA AGAAGTTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAC ATCCAGGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCTC AGGCTCTGGG TGTCTCCAGC CTCGGCAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCATTTCCG CCTCCTGTTA GAACGTCGAA CCCTGATGAG CCCTGAGCCT 
GGCTTTTTCT CCAAACTGGC AGCCATGTTT AGGGGCTTGA TCTTTTCCAG CAACACAATC 
AGGAACTCAA AGGTCCAACA GGAGCGCTCT ACAAGCTACC CGTCCCTCCT CAGCCACATG 
ACTTCCATGT ACCTCAACAC ACCTCCTCTG GTCTTACCGG TGGCCAAGAG GGAGAGCACC 
AGCCCCACTC TGCACTCTTT CCACCCTCTG GCTTCTCCGT TGCCCTGCGA TTTCCATCTG 
CTCAATCTGC GCATGCTCCC CGCCGAGGAC ACCTTGCCCG CGGCTGATGC TGCTCTCATC 
CTACACCGCA AGGGTTTTGA CTGTGGCCTT GAAGCCAAGA ACCTGGGCTT CAACTGTACC 
ACAAGCCAAG GCAAGCTGGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT GCTATTCCTG 
CAGCCCACCT CTTTGACTTT GCTATACCCT CTGGCCTCGC CCTCCAACAG CACTGACATC 
TCTCTGGAGC CCATGGAGAT CAGCACCTTC CGCCTGCGCT TGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2a2 ( XM_008759530.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757752.1 Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), transcript variant X4, mRNA.

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CloneID	ORa46659
Clone ID Related Accession (Same CDS sequence)	XM_008759530.2
Accession Version	XM_008759530.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-mannosidase 2x isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGC GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCCCTGTACC TAATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT TTCTGTGCTA CAGAACCGGA TCGAACAGCT GGAACAGCTG 
CTGGAAGAAA ACCATGAGAT CATAAGCCAT ATCAAGGACT CTGTGCTGGA ACTGACAGCC 
AATGCGGAGG GCCCACCAGC CCTGCTGCCC TACCACACAG CCAACGGCTC CTGGGCTGTG 
CTCCCCGAGC CCCGGCCCAG CTTCTTCTCT GTATCCCCTG AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAGCTACAG ATGTTAACTG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGCGGCGT GTGGAGGCAA GGCTTCGACA TCTCCTACAG CCCAAATGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCTCACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAAACCCAAC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGATCC CCGACGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAGAAA AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AAATTGCAAC GGGTGGATGG GTGATGCCAG ATGAGGCCAA CTCCCATTAC 
TTTGCCCTGG TCGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACTCCTCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTA ATTCAGAGGG TGCATTACGC CATCAAGAAG 
CACTTTGCGG CCACTCACAG CCTGGAGTTC ATGTGGAGGC AGACATGGGA TTCAGACTCC 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ACGACGTCCC ACACACCTGT 
GGCCCTGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGTCCTT GGAAGGTGCC GCCGCGGGCT ATCACAGAGG CCAACGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TTCGAAGCAG TGTCCTCCTT 
GTGCCGCTGG GTGATGACTT CCGATATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTATCAAC GGCTCTTTGA CTTCCTCAAC AGCAAGCCGG AGTTCCACGT ACAGGCACAG 
TTTGGGACCC TCTCTGAGTA TTTTGATGCC CTGTATAAGA GGACAGGAGT GGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTATGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCC CGGCCTTTCT ATAAGAGCTT GGACCGCGTG 
CTAGAAACTC ACCTTCGTGG GGCAGAGGTT CTATACAGCC TGGCTTTGGC GCATGCCCGC 
CGTTCTGGAC TGACTGGCCA GTATCCGCTG TCTGATTACG CTGTCCTGAC GGAAGCTCGT 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GAACTGCCAA GGAGGCAGTT 
GTAGTAGACT ATGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTAAAGCA GGTCATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC AAGGAGACCT ACAGCTTTGA CCCTAGGGCA 
CCCTTCCTCC AAATGGATGA CAGTCGAGTA AGTCACGATG CCCTGCCTGA GCGCACGGTG 
ATCCGTCTGG ACTCCTCACC CAGGTTTGTG GTGGTCTTTA ACCCACTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCA CCCCGAGTGC GAGTGCTTTC AGAGGAGGGT 
CAGCCCTTGT CTGTGCAGAT CAGTGTGCAG TGGAGCTCCG CCACCAACAT GGTCCCCGAT 
GTCTACCAGG TGTCAGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGACC CTACACACTG CAGTCTTCGG TGCATGTCTA CCTGAACGGC 
GTGAAACTGT CTGTCAGCAG GCAAACAACA TTCCCTCTCC GTGTTGTGGA CTCGGGCACC 
AGTGACTTCG CCATCAGCAA TCGATACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCG TCCGACGTGT GGACGAAGAG CAGGAACAGC AGGTGGACAT GAAGCTCTTC 
GTCTATGGAA CCCGCACATC CAAGGATAAG AGTGGTGCCT ACCTCTTCCT GCCTGATAAC 
GAGGCTAAGC CCTATGTCCC TAAGAAACCT CCTGTGCTGC GCGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGCGTA TTATGAGCAC TTTCACCAAG TGATTCGACT TTACAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACGTG TCGTTCCAGG TGGACATCAG GGACTACGTG 
AACAAGGAGC TAGCCCTGCG CATCCACACA GACATCGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGGT ACAGCCCCGG AAGTATCTGA AGAAGTTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAC ATCCAGGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCTC AGGCTCTGGG TGTCTCCAGC CTCGGCAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCATTTCCG CCTCCTGTTA GAACGTCGAA CCCTGATGAG CCCTGAGGTC 
CAACAGGAGC GCTCTACAAG CTACCCGTCC CTCCTCAGCC ACATGACTTC CATGTACCTC 
AACACACCTC CTCTGGTCTT ACCGGTGGCC AAGAGGGAGA GCACCAGCCC CACTCTGCAC 
TCTTTCCACC CTCTGGCTTC TCCGTTGCCC TGCGATTTCC ATCTGCTCAA TCTGCGCATG 
CTCCCCGCCG AGGACACCTT GCCCGCGGCT GATGCTGCTC TCATCCTACA CCGCAAGGGT 
TTTGACTGTG GCCTTGAAGC CAAGAACCTG GGCTTCAACT GTACCACAAG CCAAGGCAAG 
CTGGCCCTGG GGAGCCTCTT CCATGGCCTG GATGTGCTAT TCCTGCAGCC CACCTCTTTG 
ACTTTGCTAT ACCCTCTGGC CTCGCCCTCC AACAGCACTG ACATCTCTCT GGAGCCCATG 
GAGATCAGCA CCTTCCGCCT GCGCTTGGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757752.1
CDS	600..4052
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANAEGPPALLPYHTANGSWAVLPEPRPSFFSVSPEDCQFALGGRGQKPELQMLTVSEDLPFDNVEGGVWRQGFDISYSPNDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRRFLWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALVDQLIEGHQWLERNLGATPRSGWAVDPFGHSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVADRAALLLDQYRKKSRLFRSSVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPEFHVQAQFGTLSEYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLETHLRGAEVLYSLALAHARRSGLTGQYPLSDYAVLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIINAAHYLVLGDKETYSFDPRAPFLQMDDSRVSHDALPERTVIRLDSSPRFVVVFNPLEQERLSVVSLLVNSPRVRVLSEEGQPLSVQISVQWSSATNMVPDVYQVSVPVRLPALGLGVLQLQPDLDGPYTLQSSVHVYLNGVKLSVSRQTTFPLRVVDSGTSDFAISNRYMQVWFSGLTGLLKSVRRVDEEQEQQVDMKLFVYGTRTSKDKSGAYLFLPDNEAKPYVPKKPPVLRVTEGPFFSEVAAYYEHFHQVIRLYNLPGVEGLSLDVSFQVDIRDYVNKELALRIHTDIDSQGTFFTDLNGFQVQPRKYLKKLPLQANFYPMPVMAYIQDSQRRLTLHTAQALGVSSLGNGQLEVILDRRLMQDDNRGLGQGLKDNKITCNHFRLLLERRTLMSPEVQQERSTSYPSLLSHMTSMYLNTPPLVLPVAKRESTSPTLHSFHPLASPLPCDFHLLNLRMLPAEDTLPAADAALILHRKGFDCGLEAKNLGFNCTTSQGKLALGSLFHGLDVLFLQPTSLTLLYPLASPSNSTDISLEPMEISTFRLRLG

Target ORF information:

RefSeq Version	XM_008759530.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosidase, alpha, class 2A, member 2 (Man2a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759530.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGC GGGGCTGCTA TCTTCTGTGT GGCCGTCTTT 
TCCCTGTACC TAATGCTGGA CCGAGTGCAG CATGATCCTG CCAGACACCA GAATGGTGGG 
AACTTCCCCA GGAGCCAAAT TTCTGTGCTA CAGAACCGGA TCGAACAGCT GGAACAGCTG 
CTGGAAGAAA ACCATGAGAT CATAAGCCAT ATCAAGGACT CTGTGCTGGA ACTGACAGCC 
AATGCGGAGG GCCCACCAGC CCTGCTGCCC TACCACACAG CCAACGGCTC CTGGGCTGTG 
CTCCCCGAGC CCCGGCCCAG CTTCTTCTCT GTATCCCCTG AGGACTGCCA GTTTGCTTTG 
GGGGGCCGGG GTCAGAAGCC AGAGCTACAG ATGTTAACTG TGTCTGAGGA TTTGCCGTTT 
GACAATGTGG AGGGCGGCGT GTGGAGGCAA GGCTTCGACA TCTCCTACAG CCCAAATGAC 
TGGGATGCTG AAGACCTGCA GGTGTTTGTG GTGCCTCACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA CTACACGGAA CAAACCCAAC ACATCCTCAA CAGCATGGTG 
TCCAAGCTGC AGGAAGATCC CCGACGGCGC TTTCTCTGGG CAGAAGTCTC CTTCTTCGCC 
AAGTGGTGGG ACAACATCAG TGCCCAGAAA AGGGCAGCAG TTCGAAGGCT GGTGGGAAAT 
GGGCAGCTGG AAATTGCAAC GGGTGGATGG GTGATGCCAG ATGAGGCCAA CTCCCATTAC 
TTTGCCCTGG TCGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAGAAA CCTGGGTGCA 
ACTCCTCGCT CGGGCTGGGC AGTGGACCCC TTTGGACACA GCTCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTA ATTCAGAGGG TGCATTACGC CATCAAGAAG 
CACTTTGCGG CCACTCACAG CCTGGAGTTC ATGTGGAGGC AGACATGGGA TTCAGACTCC 
AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ACGACGTCCC ACACACCTGT 
GGCCCTGATC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GTCTGCCGGG TGGGAGAATC 
AATTGTCCTT GGAAGGTGCC GCCGCGGGCT ATCACAGAGG CCAACGTGGC AGACAGGGCA 
GCCCTGCTCC TGGACCAGTA CCGGAAGAAG TCCCGGCTGT TTCGAAGCAG TGTCCTCCTT 
GTGCCGCTGG GTGATGACTT CCGATATGAC AAGCCCCAGG AATGGGATGC CCAGTTCTTC 
AACTATCAAC GGCTCTTTGA CTTCCTCAAC AGCAAGCCGG AGTTCCACGT ACAGGCACAG 
TTTGGGACCC TCTCTGAGTA TTTTGATGCC CTGTATAAGA GGACAGGAGT GGAGCCTGGT 
GCCCGGCCTC CAGGGTTTCC TGTGCTGAGT GGGGACTTCT TCTCCTATGC TGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCC CGGCCTTTCT ATAAGAGCTT GGACCGCGTG 
CTAGAAACTC ACCTTCGTGG GGCAGAGGTT CTATACAGCC TGGCTTTGGC GCATGCCCGC 
CGTTCTGGAC TGACTGGCCA GTATCCGCTG TCTGATTACG CTGTCCTGAC GGAAGCTCGT 
CGTACACTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GAACTGCCAA GGAGGCAGTT 
GTAGTAGACT ATGGGGTCAG GTTGCTGCGT TCCCTGGTCA GCCTAAAGCA GGTCATCATC 
AATGCTGCCC ACTACCTGGT GCTGGGGGAC AAGGAGACCT ACAGCTTTGA CCCTAGGGCA 
CCCTTCCTCC AAATGGATGA CAGTCGAGTA AGTCACGATG CCCTGCCTGA GCGCACGGTG 
ATCCGTCTGG ACTCCTCACC CAGGTTTGTG GTGGTCTTTA ACCCACTGGA ACAGGAGCGG 
CTCAGTGTGG TGTCCCTGCT GGTCAACTCA CCCCGAGTGC GAGTGCTTTC AGAGGAGGGT 
CAGCCCTTGT CTGTGCAGAT CAGTGTGCAG TGGAGCTCCG CCACCAACAT GGTCCCCGAT 
GTCTACCAGG TGTCAGTGCC TGTCCGCCTG CCAGCCCTGG GCCTGGGTGT GCTGCAGCTG 
CAGCCAGATC TCGATGGACC CTACACACTG CAGTCTTCGG TGCATGTCTA CCTGAACGGC 
GTGAAACTGT CTGTCAGCAG GCAAACAACA TTCCCTCTCC GTGTTGTGGA CTCGGGCACC 
AGTGACTTCG CCATCAGCAA TCGATACATG CAGGTCTGGT TCTCCGGCCT TACTGGGCTT 
CTCAAGAGCG TCCGACGTGT GGACGAAGAG CAGGAACAGC AGGTGGACAT GAAGCTCTTC 
GTCTATGGAA CCCGCACATC CAAGGATAAG AGTGGTGCCT ACCTCTTCCT GCCTGATAAC 
GAGGCTAAGC CCTATGTCCC TAAGAAACCT CCTGTGCTGC GCGTCACCGA AGGCCCTTTC 
TTCTCAGAGG TGGCTGCGTA TTATGAGCAC TTTCACCAAG TGATTCGACT TTACAACCTG 
CCAGGGGTAG AGGGGCTGTC TCTGGACGTG TCGTTCCAGG TGGACATCAG GGACTACGTG 
AACAAGGAGC TAGCCCTGCG CATCCACACA GACATCGACA GCCAGGGCAC TTTCTTCACA 
GACCTCAATG GCTTTCAGGT ACAGCCCCGG AAGTATCTGA AGAAGTTGCC CCTGCAGGCT 
AATTTCTACC CTATGCCAGT CATGGCCTAC ATCCAGGATT CCCAGAGGCG CCTCACGCTG 
CACACTGCTC AGGCTCTGGG TGTCTCCAGC CTCGGCAATG GCCAGCTGGA GGTGATCTTG 
GACCGAAGGC TAATGCAGGA TGACAACCGG GGACTAGGCC AAGGGCTCAA AGACAACAAG 
ATCACCTGCA ACCATTTCCG CCTCCTGTTA GAACGTCGAA CCCTGATGAG CCCTGAGGTC 
CAACAGGAGC GCTCTACAAG CTACCCGTCC CTCCTCAGCC ACATGACTTC CATGTACCTC 
AACACACCTC CTCTGGTCTT ACCGGTGGCC AAGAGGGAGA GCACCAGCCC CACTCTGCAC 
TCTTTCCACC CTCTGGCTTC TCCGTTGCCC TGCGATTTCC ATCTGCTCAA TCTGCGCATG 
CTCCCCGCCG AGGACACCTT GCCCGCGGCT GATGCTGCTC TCATCCTACA CCGCAAGGGT 
TTTGACTGTG GCCTTGAAGC CAAGAACCTG GGCTTCAACT GTACCACAAG CCAAGGCAAG 
CTGGCCCTGG GGAGCCTCTT CCATGGCCTG GATGTGCTAT TCCTGCAGCC CACCTCTTTG 
ACTTTGCTAT ACCCTCTGGC CTCGCCCTCC AACAGCACTG ACATCTCTCT GGAGCCCATG 
GAGATCAGCA CCTTCCGCCT GCGCTTGGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Essential and mutually compensatory roles of {alpha}-mannosidase II and {alpha}-mannosidase IIx in N-glycan processing in vivo in mice.
Proceedings of the National Academy of Sciences of the United States of America103(24)8983-8(2006 Jun)
Akama TO,Nakagawa H,Wong NK,Sutton-Smith M,Dell A,Morris HR,Nakayama J,Nishimura S,Pai A,Moremen KW,Marth JD,Fukuda MN

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Man2a2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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