alpha-Man-IIa cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	alpha-Man-IIa
Entrez Gene ID	41126
Full Name	alpha-Mannosidase class II a
Synonyms	CG18474,CG18802,CG8139,DM-GII.1,Dmel\CG18802,GMII,GmII,MAN-2,Man,Man-II,alpha-Man-II,dGMII,dGMIIb
General protein information	Preferred Names alpha-Mannosidase class II a Names CG18802 gene product from transcript CG18802-RB CG18802-PA CG18802-PB alpha mannosidase II alpha-Man-IIa-PA alpha-Man-IIa-PB alpha-mannosidase 2 alpha-mannosidase II alpha-mannosidase-II chain A, golgi Alpha-Mannosidase Ii golgi Man II golgi alpha-mannosidase golgi alpha-mannosidase II golgi mannosidase II mannosidase II
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85D15-85D16

mRNA and Protein(s)

mRNA	Protein	Name
NM_079567.3	NP_524291.2	alpha-Mannosidase class II a, isoform A
NM_001275484.1	NP_001262413.1	alpha-Mannosidase class II a, isoform B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Man2a2	NP_001100997.1
Oryza sativa (rice)	Os06g0245700	NP_001057277.2
Xenopus tropicalis (tropical clawed frog)	man2a2	NP_001006777.1
Pan troglodytes (chimpanzee)	MAN2A2	XP_001169951.1
Gallus gallus (chicken)	MAN2A2	XP_001233608.2
Danio rerio (zebrafish)	man2a2	XP_005163040.1
Homo sapiens (human)	MAN2A2	NP_006113.2
Bos taurus (cattle)	MAN2A2	NP_001179570.1
Mus musculus (house mouse)	Man2a2	NP_766491.2
Drosophila melanogaster (fruit fly)	alpha-Man-II	NP_524291.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004020	XP_318477.4
Canis lupus familiaris (dog)	MAN2A2	XP_545863.3
Arabidopsis thaliana (thale cress)	GMII	NP_196999.1
Caenorhabditis elegans (roundworm)	aman-2	NP_505995.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data show that the Drosophila metallophosphoesterase-dephosphorylated alpha-Man-II is required for the removal of the Rh1 oligosaccharide chain.
Title: A Drosophila metallophosphoesterase mediates deglycosylation of rhodopsin.

high-resolution crystal structure of a nucleophile mutant of Drosophila melanogaster GMII bound to its natural oligosaccharide substrate and an oligosaccharide precursor as well as the structure of the unliganded mutant.
Title: Golgi alpha-mannosidase II cleaves two sugars sequentially in the same catalytic site.

Drosophila melanogaster Golgi alpha-mannosidase II mechanism seen through the structural analysis of covalent reaction intermediates
Title: Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates.

The following alpha-Man-IIa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alpha-Man-IIa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04327	NM_001275484.1 Latest version!	Drosophila melanogaster alpha-Mannosidase class II a (alpha-Man-IIa), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa08207	NM_079567.3 Latest version!	Drosophila melanogaster alpha-Mannosidase class II a (alpha-Man-IIa), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alpha-Man-IIa ( NM_001275484.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262413.1 Drosophila melanogaster alpha-Mannosidase class II a (alpha-Man-IIa), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04327
Clone ID Related Accession (Same CDS sequence)	NM_001275484.1
Accession Version	NM_001275484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha-Mannosidase class II a, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGAA TACGTCGGCG GTTCGCTTTG GTAATTTGCT CCGGCTGCCT GCTGGTTTTC 
CTCAGCCTGT ACATAATCCT CAATTTTGCG GCGCCGGCAG CCACCCAGAT AAAGCAGCCC 
AACTATGAGA ACATTGAGAA CAAGCTGCAT GAGCTGGAAA ATGGTTTGCA GGAGCACGGG 
GAGGAGATGC GGAATCTCAG GGCGCGTCTT GCCGAAACAT CCAATCGCGA CGATCCAATA 
AGACCTCCAC TTAAAGTGGC TCGTTCCCCG AGGCCAGGGC AATGCCAAGA TGTGGTCCAA 
GACGTGCCCA ATGTGGATGT ACAGATGCTG GAGCTATACG ATCGCATGTC CTTCAAGGAC 
ATAGATGGAG GCGTGTGGAA ACAGGGCTGG AACATTAAGT ACGATCCACT GAAGTACAAC 
GCCCATCACA AACTAAAAGT CTTCGTTGTG CCGCACTCGC ACAACGATCC TGGATGGATT 
CAGACGTTTG AGGAATACTA CCAGCACGAC ACCAAGCACA TCCTGTCCAA TGCACTACGG 
CATCTGCACG ACAATCCCGA GATGAAGTTC ATCTGGGCGG AAATCTCCTA CTTTGCTCGG 
TTCTATCACG ATTTGGGAGA GAACAAAAAG CTGCAGATGA AGTCCATTGT AAAGAATGGA 
CAGTTGGAAT TTGTGACTGG AGGATGGGTA ATGCCGGACG AGGCCAACTC CCACTGGCGA 
AACGTACTGC TGCAGCTGAC CGAAGGGCAA ACATGGTTGA AGCAATTCAT GAATGTCACA 
CCCACTGCTT CCTGGGCCAT CGATCCCTTC GGACACAGTC CCACTATGCC GTACATTTTG 
CAGAAGAGTG GTTTCAAGAA TATGCTTATC CAAAGGACGC ACTATTCGGT TAAGAAGGAA 
CTGGCCCAAC AGCGACAGCT TGAGTTCCTG TGGCGCCAGA TCTGGGACAA CAAAGGGGAC 
ACAGCTCTCT TCACCCACAT GATGCCCTTC TACTCGTACG ACATTCCTCA CACCTGTGGT 
CCAGATCCCA AGGTTTGCTG TCAGTTCGAT TTCAAACGAA TGGGCTCCTT CGGTTTGAGC 
TGTCCATGGA AGGTGCCGCC GCGTACAATC AGTGATCAAA ATGTGGCAGC ACGCTCAGAT 
CTGCTGGTTG ATCAGTGGAA GAAGAAGGCC GAGCTGTATC GCACGAACGT GCTGCTGATT 
CCGTTGGGTG ACGACTTCCG CTTCAAGCAG AACACCGAGT GGGATGTGCA GCGCGTGAAC 
TACGAAAGGC TGTTCGAACA CATCAACAGC CAGGCCCACT TCAATGTCCA GGCGCAGTTC 
GGCACACTGC AGGAATACTT TGATGCAGTG CACCAGGCGG AAAGGGCGGG ACAAGCCGAG 
TTTCCCACGC TAAGCGGTGA CTTTTTCACA TACGCCGATC GATCGGATAA CTATTGGAGT 
GGCTACTACA CATCCCGCCC GTATCATAAG CGCATGGACC GCGTCCTGAT GCACTATGTA 
CGTGCAGCAG AAATGCTTTC CGCCTGGCAC TCCTGGGACG GTATGGCCCG CATCGAGGAA 
CGTCTGGAGC AGGCCCGCAG GGAGCTGTCA TTGTTCCAGC ACCACGACGG TATAACTGGC 
ACAGCAAAAA CGCACGTAGT CGTCGACTAC GAGCAACGCA TGCAGGAAGC TTTAAAAGCC 
TGTCAAATGG TAATGCAACA GTCGGTCTAC CGATTGCTGA CAAAGCCCTC CATCTACAGT 
CCGGACTTCA GTTTCTCGTA CTTTACGCTC GACGACTCCC GCTGGCCAGG ATCTGGTGTG 
GAGGACAGTC GAACCACCAT AATACTGGGC GAGGATATAC TGCCCTCCAA GCATGTGGTG 
ATGCACAACA CCCTGCCCCA CTGGCGGGAG CAGCTGGTGG ACTTTTATGT ATCCAGTCCG 
TTTGTAAGCG TTACCGACTT GGCAAACAAT CCGGTGGAGG CTCAGGTGTC CCCGGTGTGG 
AGCTGGCACC ACGACACACT CACAAAGACT ATCCACCCAC AAGGCTCCAC CACCAAGTAC 
CGCATCATCT TCAAGGCTCG GGTGCCGCCC ATGGGCTTGG CCACCTACGT TTTAACCATC 
TCCGATTCCA AGCCAGAGCA CACCTCGTAT GCATCGAATC TCTTGCTCCG TAAAAACCCG 
ACTTCGTTAC CATTGGGCCA ATATCCGGAG GATGTGAAGT TTGGCGATCC TCGAGAGATC 
TCATTGCGGG TTGGTAACGG ACCCACCTTG GCCTTTTCGG AGCAGGGTCT CCTTAAGTCC 
ATTCAGCTTA CTCAGGATAG CCCACATGTA CCGGTGCACT TCAAGTTCCT CAAGTATGGC 
GTTCGATCGC ATGGCGATAG ATCCGGTGCC TATCTGTTCC TGCCCAATGG ACCAGCTTCG 
CCAGTCGAGC TTGGCCAGCC AGTGGTCCTG GTGACTAAGG GCAAACTGGA GTCGTCCGTG 
AGCGTGGGAC TTCCGAGCGT GGTGCACCAG ACGATAATGC GCGGTGGTGC ACCTGAGATT 
CGCAATCTGG TGGATATAGG CTCACTGGAC AACACGGAGA TCGTGATGCG CTTGGAGACG 
CATATCGACA GCGGCGATAT CTTCTACACG GATCTCAATG GATTGCAATT TATCAAGAGG 
CGGCGTTTGG ACAAATTACC TTTGCAGGCC AACTATTATC CCATACCTTC TGGTATGTTC 
ATTGAGGATG CCAATACGCG ACTCACTCTC CTCACGGGTC AACCGCTGGG TGGATCTTCT 
CTGGCCTCGG GCGAGCTAGA GATTATGCAA GATCGTCGCC TGGCCAGCGA TGATGAACGC 
GGCCTGGGAC AGGGTGTTTT GGACAACAAG CCGGTGCTGC ATATTTATCG GCTGGTGCTG 
GAGAAGGTTA ACAACTGTGT CCGACCGTCA GAGCTTCATC CTGCCGGCTA TTTGACAAGT 
GCCGCTCACA AAGCATCGCA GTCACTGCTG GATCCACTGG ACAAGTTTAT ATTCGCTGAA 
AATGAGTGGA TCGGGGCACA GGGGCAATTT GGTGGCGATC ATCCTTCGGC TCGTGAGGAT 
CTCGATGTGT CGGTGATGAG ACGCTTAACC AAGAGCTCGG CCAAAACCCA GCGAGTAGGC 
TACGTTCTGC ACCGCACCAA TCTGATGCAA TGCGGCACTC CAGAGGAGCA TACACAGAAG 
CTGGATGTGT GCCACCTACT GCCGAATGTG GCGAGATGCG AGCGCACGAC GCTGACTTTC 
CTGCAGAATT TGGAGCACTT GGATGGCATG GTGGCGCCGG AAGTGTGCCC CATGGAAACC 
GCCGCTTATG TGAGCAGTCA CTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262413.1
CDS	147..3476
Translation	MLRIRRRFALVICSGCLLVFLSLYIILNFAAPAATQIKQPNYENIENKLHELENGLQEHGEEMRNLRARLAETSNRDDPIRPPLKVARSPRPGQCQDVVQDVPNVDVQMLELYDRMSFKDIDGGVWKQGWNIKYDPLKYNAHHKLKVFVVPHSHNDPGWIQTFEEYYQHDTKHILSNALRHLHDNPEMKFIWAEISYFARFYHDLGENKKLQMKSIVKNGQLEFVTGGWVMPDEANSHWRNVLLQLTEGQTWLKQFMNVTPTASWAIDPFGHSPTMPYILQKSGFKNMLIQRTHYSVKKELAQQRQLEFLWRQIWDNKGDTALFTHMMPFYSYDIPHTCGPDPKVCCQFDFKRMGSFGLSCPWKVPPRTISDQNVAARSDLLVDQWKKKAELYRTNVLLIPLGDDFRFKQNTEWDVQRVNYERLFEHINSQAHFNVQAQFGTLQEYFDAVHQAERAGQAEFPTLSGDFFTYADRSDNYWSGYYTSRPYHKRMDRVLMHYVRAAEMLSAWHSWDGMARIEERLEQARRELSLFQHHDGITGTAKTHVVVDYEQRMQEALKACQMVMQQSVYRLLTKPSIYSPDFSFSYFTLDDSRWPGSGVEDSRTTIILGEDILPSKHVVMHNTLPHWREQLVDFYVSSPFVSVTDLANNPVEAQVSPVWSWHHDTLTKTIHPQGSTTKYRIIFKARVPPMGLATYVLTISDSKPEHTSYASNLLLRKNPTSLPLGQYPEDVKFGDPREISLRVGNGPTLAFSEQGLLKSIQLTQDSPHVPVHFKFLKYGVRSHGDRSGAYLFLPNGPASPVELGQPVVLVTKGKLESSVSVGLPSVVHQTIMRGGAPEIRNLVDIGSLDNTEIVMRLETHIDSGDIFYTDLNGLQFIKRRRLDKLPLQANYYPIPSGMFIEDANTRLTLLTGQPLGGSSLASGELEIMQDRRLASDDERGLGQGVLDNKPVLHIYRLVLEKVNNCVRPSELHPAGYLTSAAHKASQSLLDPLDKFIFAENEWIGAQGQFGGDHPSAREDLDVSVMRRLTKSSAKTQRVGYVLHRTNLMQCGTPEEHTQKLDVCHLLPNVARCERTTLTFLQNLEHLDGMVAPEVCPMETAAYVSSHSS

Target ORF information:

RefSeq Version	NM_001275484.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha-Mannosidase class II a (alpha-Man-IIa), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275484.1

ORF Insert Sequence:

ATGTTGCGAA TACGTCGGCG GTTCGCTTTG GTAATTTGCT CCGGCTGCCT GCTGGTTTTC 
CTCAGCCTGT ACATAATCCT CAATTTTGCG GCGCCGGCAG CCACCCAGAT AAAGCAGCCC 
AACTATGAGA ACATTGAGAA CAAGCTGCAT GAGCTGGAAA ATGGTTTGCA GGAGCACGGG 
GAGGAGATGC GGAATCTCAG GGCGCGTCTT GCCGAAACAT CCAATCGCGA CGATCCAATA 
AGACCTCCAC TTAAAGTGGC TCGTTCCCCG AGGCCAGGGC AATGCCAAGA TGTGGTCCAA 
GACGTGCCCA ATGTGGATGT ACAGATGCTG GAGCTATACG ATCGCATGTC CTTCAAGGAC 
ATAGATGGAG GCGTGTGGAA ACAGGGCTGG AACATTAAGT ACGATCCACT GAAGTACAAC 
GCCCATCACA AACTAAAAGT CTTCGTTGTG CCGCACTCGC ACAACGATCC TGGATGGATT 
CAGACGTTTG AGGAATACTA CCAGCACGAC ACCAAGCACA TCCTGTCCAA TGCACTACGG 
CATCTGCACG ACAATCCCGA GATGAAGTTC ATCTGGGCGG AAATCTCCTA CTTTGCTCGG 
TTCTATCACG ATTTGGGAGA GAACAAAAAG CTGCAGATGA AGTCCATTGT AAAGAATGGA 
CAGTTGGAAT TTGTGACTGG AGGATGGGTA ATGCCGGACG AGGCCAACTC CCACTGGCGA 
AACGTACTGC TGCAGCTGAC CGAAGGGCAA ACATGGTTGA AGCAATTCAT GAATGTCACA 
CCCACTGCTT CCTGGGCCAT CGATCCCTTC GGACACAGTC CCACTATGCC GTACATTTTG 
CAGAAGAGTG GTTTCAAGAA TATGCTTATC CAAAGGACGC ACTATTCGGT TAAGAAGGAA 
CTGGCCCAAC AGCGACAGCT TGAGTTCCTG TGGCGCCAGA TCTGGGACAA CAAAGGGGAC 
ACAGCTCTCT TCACCCACAT GATGCCCTTC TACTCGTACG ACATTCCTCA CACCTGTGGT 
CCAGATCCCA AGGTTTGCTG TCAGTTCGAT TTCAAACGAA TGGGCTCCTT CGGTTTGAGC 
TGTCCATGGA AGGTGCCGCC GCGTACAATC AGTGATCAAA ATGTGGCAGC ACGCTCAGAT 
CTGCTGGTTG ATCAGTGGAA GAAGAAGGCC GAGCTGTATC GCACGAACGT GCTGCTGATT 
CCGTTGGGTG ACGACTTCCG CTTCAAGCAG AACACCGAGT GGGATGTGCA GCGCGTGAAC 
TACGAAAGGC TGTTCGAACA CATCAACAGC CAGGCCCACT TCAATGTCCA GGCGCAGTTC 
GGCACACTGC AGGAATACTT TGATGCAGTG CACCAGGCGG AAAGGGCGGG ACAAGCCGAG 
TTTCCCACGC TAAGCGGTGA CTTTTTCACA TACGCCGATC GATCGGATAA CTATTGGAGT 
GGCTACTACA CATCCCGCCC GTATCATAAG CGCATGGACC GCGTCCTGAT GCACTATGTA 
CGTGCAGCAG AAATGCTTTC CGCCTGGCAC TCCTGGGACG GTATGGCCCG CATCGAGGAA 
CGTCTGGAGC AGGCCCGCAG GGAGCTGTCA TTGTTCCAGC ACCACGACGG TATAACTGGC 
ACAGCAAAAA CGCACGTAGT CGTCGACTAC GAGCAACGCA TGCAGGAAGC TTTAAAAGCC 
TGTCAAATGG TAATGCAACA GTCGGTCTAC CGATTGCTGA CAAAGCCCTC CATCTACAGT 
CCGGACTTCA GTTTCTCGTA CTTTACGCTC GACGACTCCC GCTGGCCAGG ATCTGGTGTG 
GAGGACAGTC GAACCACCAT AATACTGGGC GAGGATATAC TGCCCTCCAA GCATGTGGTG 
ATGCACAACA CCCTGCCCCA CTGGCGGGAG CAGCTGGTGG ACTTTTATGT ATCCAGTCCG 
TTTGTAAGCG TTACCGACTT GGCAAACAAT CCGGTGGAGG CTCAGGTGTC CCCGGTGTGG 
AGCTGGCACC ACGACACACT CACAAAGACT ATCCACCCAC AAGGCTCCAC CACCAAGTAC 
CGCATCATCT TCAAGGCTCG GGTGCCGCCC ATGGGCTTGG CCACCTACGT TTTAACCATC 
TCCGATTCCA AGCCAGAGCA CACCTCGTAT GCATCGAATC TCTTGCTCCG TAAAAACCCG 
ACTTCGTTAC CATTGGGCCA ATATCCGGAG GATGTGAAGT TTGGCGATCC TCGAGAGATC 
TCATTGCGGG TTGGTAACGG ACCCACCTTG GCCTTTTCGG AGCAGGGTCT CCTTAAGTCC 
ATTCAGCTTA CTCAGGATAG CCCACATGTA CCGGTGCACT TCAAGTTCCT CAAGTATGGC 
GTTCGATCGC ATGGCGATAG ATCCGGTGCC TATCTGTTCC TGCCCAATGG ACCAGCTTCG 
CCAGTCGAGC TTGGCCAGCC AGTGGTCCTG GTGACTAAGG GCAAACTGGA GTCGTCCGTG 
AGCGTGGGAC TTCCGAGCGT GGTGCACCAG ACGATAATGC GCGGTGGTGC ACCTGAGATT 
CGCAATCTGG TGGATATAGG CTCACTGGAC AACACGGAGA TCGTGATGCG CTTGGAGACG 
CATATCGACA GCGGCGATAT CTTCTACACG GATCTCAATG GATTGCAATT TATCAAGAGG 
CGGCGTTTGG ACAAATTACC TTTGCAGGCC AACTATTATC CCATACCTTC TGGTATGTTC 
ATTGAGGATG CCAATACGCG ACTCACTCTC CTCACGGGTC AACCGCTGGG TGGATCTTCT 
CTGGCCTCGG GCGAGCTAGA GATTATGCAA GATCGTCGCC TGGCCAGCGA TGATGAACGC 
GGCCTGGGAC AGGGTGTTTT GGACAACAAG CCGGTGCTGC ATATTTATCG GCTGGTGCTG 
GAGAAGGTTA ACAACTGTGT CCGACCGTCA GAGCTTCATC CTGCCGGCTA TTTGACAAGT 
GCCGCTCACA AAGCATCGCA GTCACTGCTG GATCCACTGG ACAAGTTTAT ATTCGCTGAA 
AATGAGTGGA TCGGGGCACA GGGGCAATTT GGTGGCGATC ATCCTTCGGC TCGTGAGGAT 
CTCGATGTGT CGGTGATGAG ACGCTTAACC AAGAGCTCGG CCAAAACCCA GCGAGTAGGC 
TACGTTCTGC ACCGCACCAA TCTGATGCAA TGCGGCACTC CAGAGGAGCA TACACAGAAG 
CTGGATGTGT GCCACCTACT GCCGAATGTG GCGAGATGCG AGCGCACGAC GCTGACTTTC 
CTGCAGAATT TGGAGCACTT GGATGGCATG GTGGCGCCGG AAGTGTGCCC CATGGAAACC 
GCCGCTTATG TGAGCAGTCA CTCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alpha-Man-IIa ( NM_079567.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524291.2 Drosophila melanogaster alpha-Mannosidase class II a (alpha-Man-IIa), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa08207
Clone ID Related Accession (Same CDS sequence)	NM_079567.3
Accession Version	NM_079567.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha-Mannosidase class II a, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079567.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGAA TACGTCGGCG GTTCGCTTTG GTAATTTGCT CCGGCTGCCT GCTGGTTTTC 
CTCAGCCTGT ACATAATCCT CAATTTTGCG GCGCCGGCAG CCACCCAGAT AAAGCCCAAC 
TATGAGAACA TTGAGAACAA GCTGCATGAG CTGGAAAATG GTTTGCAGGA GCACGGGGAG 
GAGATGCGGA ATCTCAGGGC GCGTCTTGCC GAAACATCCA ATCGCGACGA TCCAATAAGA 
CCTCCACTTA AAGTGGCTCG TTCCCCGAGG CCAGGGCAAT GCCAAGATGT GGTCCAAGAC 
GTGCCCAATG TGGATGTACA GATGCTGGAG CTATACGATC GCATGTCCTT CAAGGACATA 
GATGGAGGCG TGTGGAAACA GGGCTGGAAC ATTAAGTACG ATCCACTGAA GTACAACGCC 
CATCACAAAC TAAAAGTCTT CGTTGTGCCG CACTCGCACA ACGATCCTGG ATGGATTCAG 
ACGTTTGAGG AATACTACCA GCACGACACC AAGCACATCC TGTCCAATGC ACTACGGCAT 
CTGCACGACA ATCCCGAGAT GAAGTTCATC TGGGCGGAAA TCTCCTACTT TGCTCGGTTC 
TATCACGATT TGGGAGAGAA CAAAAAGCTG CAGATGAAGT CCATTGTAAA GAATGGACAG 
TTGGAATTTG TGACTGGAGG ATGGGTAATG CCGGACGAGG CCAACTCCCA CTGGCGAAAC 
GTACTGCTGC AGCTGACCGA AGGGCAAACA TGGTTGAAGC AATTCATGAA TGTCACACCC 
ACTGCTTCCT GGGCCATCGA TCCCTTCGGA CACAGTCCCA CTATGCCGTA CATTTTGCAG 
AAGAGTGGTT TCAAGAATAT GCTTATCCAA AGGACGCACT ATTCGGTTAA GAAGGAACTG 
GCCCAACAGC GACAGCTTGA GTTCCTGTGG CGCCAGATCT GGGACAACAA AGGGGACACA 
GCTCTCTTCA CCCACATGAT GCCCTTCTAC TCGTACGACA TTCCTCACAC CTGTGGTCCA 
GATCCCAAGG TTTGCTGTCA GTTCGATTTC AAACGAATGG GCTCCTTCGG TTTGAGCTGT 
CCATGGAAGG TGCCGCCGCG TACAATCAGT GATCAAAATG TGGCAGCACG CTCAGATCTG 
CTGGTTGATC AGTGGAAGAA GAAGGCCGAG CTGTATCGCA CGAACGTGCT GCTGATTCCG 
TTGGGTGACG ACTTCCGCTT CAAGCAGAAC ACCGAGTGGG ATGTGCAGCG CGTGAACTAC 
GAAAGGCTGT TCGAACACAT CAACAGCCAG GCCCACTTCA ATGTCCAGGC GCAGTTCGGC 
ACACTGCAGG AATACTTTGA TGCAGTGCAC CAGGCGGAAA GGGCGGGACA AGCCGAGTTT 
CCCACGCTAA GCGGTGACTT TTTCACATAC GCCGATCGAT CGGATAACTA TTGGAGTGGC 
TACTACACAT CCCGCCCGTA TCATAAGCGC ATGGACCGCG TCCTGATGCA CTATGTACGT 
GCAGCAGAAA TGCTTTCCGC CTGGCACTCC TGGGACGGTA TGGCCCGCAT CGAGGAACGT 
CTGGAGCAGG CCCGCAGGGA GCTGTCATTG TTCCAGCACC ACGACGGTAT AACTGGCACA 
GCAAAAACGC ACGTAGTCGT CGACTACGAG CAACGCATGC AGGAAGCTTT AAAAGCCTGT 
CAAATGGTAA TGCAACAGTC GGTCTACCGA TTGCTGACAA AGCCCTCCAT CTACAGTCCG 
GACTTCAGTT TCTCGTACTT TACGCTCGAC GACTCCCGCT GGCCAGGATC TGGTGTGGAG 
GACAGTCGAA CCACCATAAT ACTGGGCGAG GATATACTGC CCTCCAAGCA TGTGGTGATG 
CACAACACCC TGCCCCACTG GCGGGAGCAG CTGGTGGACT TTTATGTATC CAGTCCGTTT 
GTAAGCGTTA CCGACTTGGC AAACAATCCG GTGGAGGCTC AGGTGTCCCC GGTGTGGAGC 
TGGCACCACG ACACACTCAC AAAGACTATC CACCCACAAG GCTCCACCAC CAAGTACCGC 
ATCATCTTCA AGGCTCGGGT GCCGCCCATG GGCTTGGCCA CCTACGTTTT AACCATCTCC 
GATTCCAAGC CAGAGCACAC CTCGTATGCA TCGAATCTCT TGCTCCGTAA AAACCCGACT 
TCGTTACCAT TGGGCCAATA TCCGGAGGAT GTGAAGTTTG GCGATCCTCG AGAGATCTCA 
TTGCGGGTTG GTAACGGACC CACCTTGGCC TTTTCGGAGC AGGGTCTCCT TAAGTCCATT 
CAGCTTACTC AGGATAGCCC ACATGTACCG GTGCACTTCA AGTTCCTCAA GTATGGCGTT 
CGATCGCATG GCGATAGATC CGGTGCCTAT CTGTTCCTGC CCAATGGACC AGCTTCGCCA 
GTCGAGCTTG GCCAGCCAGT GGTCCTGGTG ACTAAGGGCA AACTGGAGTC GTCCGTGAGC 
GTGGGACTTC CGAGCGTGGT GCACCAGACG ATAATGCGCG GTGGTGCACC TGAGATTCGC 
AATCTGGTGG ATATAGGCTC ACTGGACAAC ACGGAGATCG TGATGCGCTT GGAGACGCAT 
ATCGACAGCG GCGATATCTT CTACACGGAT CTCAATGGAT TGCAATTTAT CAAGAGGCGG 
CGTTTGGACA AATTACCTTT GCAGGCCAAC TATTATCCCA TACCTTCTGG TATGTTCATT 
GAGGATGCCA ATACGCGACT CACTCTCCTC ACGGGTCAAC CGCTGGGTGG ATCTTCTCTG 
GCCTCGGGCG AGCTAGAGAT TATGCAAGAT CGTCGCCTGG CCAGCGATGA TGAACGCGGC 
CTGGGACAGG GTGTTTTGGA CAACAAGCCG GTGCTGCATA TTTATCGGCT GGTGCTGGAG 
AAGGTTAACA ACTGTGTCCG ACCGTCAGAG CTTCATCCTG CCGGCTATTT GACAAGTGCC 
GCTCACAAAG CATCGCAGTC ACTGCTGGAT CCACTGGACA AGTTTATATT CGCTGAAAAT 
GAGTGGATCG GGGCACAGGG GCAATTTGGT GGCGATCATC CTTCGGCTCG TGAGGATCTC 
GATGTGTCGG TGATGAGACG CTTAACCAAG AGCTCGGCCA AAACCCAGCG AGTAGGCTAC 
GTTCTGCACC GCACCAATCT GATGCAATGC GGCACTCCAG AGGAGCATAC ACAGAAGCTG 
GATGTGTGCC ACCTACTGCC GAATGTGGCG AGATGCGAGC GCACGACGCT GACTTTCCTG 
CAGAATTTGG AGCACTTGGA TGGCATGGTG GCGCCGGAAG TGTGCCCCAT GGAAACCGCC 
GCTTATGTGA GCAGTCACTC AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524291.2
CDS	147..3473
Translation	MLRIRRRFALVICSGCLLVFLSLYIILNFAAPAATQIKPNYENIENKLHELENGLQEHGEEMRNLRARLAETSNRDDPIRPPLKVARSPRPGQCQDVVQDVPNVDVQMLELYDRMSFKDIDGGVWKQGWNIKYDPLKYNAHHKLKVFVVPHSHNDPGWIQTFEEYYQHDTKHILSNALRHLHDNPEMKFIWAEISYFARFYHDLGENKKLQMKSIVKNGQLEFVTGGWVMPDEANSHWRNVLLQLTEGQTWLKQFMNVTPTASWAIDPFGHSPTMPYILQKSGFKNMLIQRTHYSVKKELAQQRQLEFLWRQIWDNKGDTALFTHMMPFYSYDIPHTCGPDPKVCCQFDFKRMGSFGLSCPWKVPPRTISDQNVAARSDLLVDQWKKKAELYRTNVLLIPLGDDFRFKQNTEWDVQRVNYERLFEHINSQAHFNVQAQFGTLQEYFDAVHQAERAGQAEFPTLSGDFFTYADRSDNYWSGYYTSRPYHKRMDRVLMHYVRAAEMLSAWHSWDGMARIEERLEQARRELSLFQHHDGITGTAKTHVVVDYEQRMQEALKACQMVMQQSVYRLLTKPSIYSPDFSFSYFTLDDSRWPGSGVEDSRTTIILGEDILPSKHVVMHNTLPHWREQLVDFYVSSPFVSVTDLANNPVEAQVSPVWSWHHDTLTKTIHPQGSTTKYRIIFKARVPPMGLATYVLTISDSKPEHTSYASNLLLRKNPTSLPLGQYPEDVKFGDPREISLRVGNGPTLAFSEQGLLKSIQLTQDSPHVPVHFKFLKYGVRSHGDRSGAYLFLPNGPASPVELGQPVVLVTKGKLESSVSVGLPSVVHQTIMRGGAPEIRNLVDIGSLDNTEIVMRLETHIDSGDIFYTDLNGLQFIKRRRLDKLPLQANYYPIPSGMFIEDANTRLTLLTGQPLGGSSLASGELEIMQDRRLASDDERGLGQGVLDNKPVLHIYRLVLEKVNNCVRPSELHPAGYLTSAAHKASQSLLDPLDKFIFAENEWIGAQGQFGGDHPSAREDLDVSVMRRLTKSSAKTQRVGYVLHRTNLMQCGTPEEHTQKLDVCHLLPNVARCERTTLTFLQNLEHLDGMVAPEVCPMETAAYVSSHSS

Target ORF information:

RefSeq Version	NM_079567.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha-Mannosidase class II a (alpha-Man-IIa), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079567.3

ORF Insert Sequence:

ATGTTGCGAA TACGTCGGCG GTTCGCTTTG GTAATTTGCT CCGGCTGCCT GCTGGTTTTC 
CTCAGCCTGT ACATAATCCT CAATTTTGCG GCGCCGGCAG CCACCCAGAT AAAGCCCAAC 
TATGAGAACA TTGAGAACAA GCTGCATGAG CTGGAAAATG GTTTGCAGGA GCACGGGGAG 
GAGATGCGGA ATCTCAGGGC GCGTCTTGCC GAAACATCCA ATCGCGACGA TCCAATAAGA 
CCTCCACTTA AAGTGGCTCG TTCCCCGAGG CCAGGGCAAT GCCAAGATGT GGTCCAAGAC 
GTGCCCAATG TGGATGTACA GATGCTGGAG CTATACGATC GCATGTCCTT CAAGGACATA 
GATGGAGGCG TGTGGAAACA GGGCTGGAAC ATTAAGTACG ATCCACTGAA GTACAACGCC 
CATCACAAAC TAAAAGTCTT CGTTGTGCCG CACTCGCACA ACGATCCTGG ATGGATTCAG 
ACGTTTGAGG AATACTACCA GCACGACACC AAGCACATCC TGTCCAATGC ACTACGGCAT 
CTGCACGACA ATCCCGAGAT GAAGTTCATC TGGGCGGAAA TCTCCTACTT TGCTCGGTTC 
TATCACGATT TGGGAGAGAA CAAAAAGCTG CAGATGAAGT CCATTGTAAA GAATGGACAG 
TTGGAATTTG TGACTGGAGG ATGGGTAATG CCGGACGAGG CCAACTCCCA CTGGCGAAAC 
GTACTGCTGC AGCTGACCGA AGGGCAAACA TGGTTGAAGC AATTCATGAA TGTCACACCC 
ACTGCTTCCT GGGCCATCGA TCCCTTCGGA CACAGTCCCA CTATGCCGTA CATTTTGCAG 
AAGAGTGGTT TCAAGAATAT GCTTATCCAA AGGACGCACT ATTCGGTTAA GAAGGAACTG 
GCCCAACAGC GACAGCTTGA GTTCCTGTGG CGCCAGATCT GGGACAACAA AGGGGACACA 
GCTCTCTTCA CCCACATGAT GCCCTTCTAC TCGTACGACA TTCCTCACAC CTGTGGTCCA 
GATCCCAAGG TTTGCTGTCA GTTCGATTTC AAACGAATGG GCTCCTTCGG TTTGAGCTGT 
CCATGGAAGG TGCCGCCGCG TACAATCAGT GATCAAAATG TGGCAGCACG CTCAGATCTG 
CTGGTTGATC AGTGGAAGAA GAAGGCCGAG CTGTATCGCA CGAACGTGCT GCTGATTCCG 
TTGGGTGACG ACTTCCGCTT CAAGCAGAAC ACCGAGTGGG ATGTGCAGCG CGTGAACTAC 
GAAAGGCTGT TCGAACACAT CAACAGCCAG GCCCACTTCA ATGTCCAGGC GCAGTTCGGC 
ACACTGCAGG AATACTTTGA TGCAGTGCAC CAGGCGGAAA GGGCGGGACA AGCCGAGTTT 
CCCACGCTAA GCGGTGACTT TTTCACATAC GCCGATCGAT CGGATAACTA TTGGAGTGGC 
TACTACACAT CCCGCCCGTA TCATAAGCGC ATGGACCGCG TCCTGATGCA CTATGTACGT 
GCAGCAGAAA TGCTTTCCGC CTGGCACTCC TGGGACGGTA TGGCCCGCAT CGAGGAACGT 
CTGGAGCAGG CCCGCAGGGA GCTGTCATTG TTCCAGCACC ACGACGGTAT AACTGGCACA 
GCAAAAACGC ACGTAGTCGT CGACTACGAG CAACGCATGC AGGAAGCTTT AAAAGCCTGT 
CAAATGGTAA TGCAACAGTC GGTCTACCGA TTGCTGACAA AGCCCTCCAT CTACAGTCCG 
GACTTCAGTT TCTCGTACTT TACGCTCGAC GACTCCCGCT GGCCAGGATC TGGTGTGGAG 
GACAGTCGAA CCACCATAAT ACTGGGCGAG GATATACTGC CCTCCAAGCA TGTGGTGATG 
CACAACACCC TGCCCCACTG GCGGGAGCAG CTGGTGGACT TTTATGTATC CAGTCCGTTT 
GTAAGCGTTA CCGACTTGGC AAACAATCCG GTGGAGGCTC AGGTGTCCCC GGTGTGGAGC 
TGGCACCACG ACACACTCAC AAAGACTATC CACCCACAAG GCTCCACCAC CAAGTACCGC 
ATCATCTTCA AGGCTCGGGT GCCGCCCATG GGCTTGGCCA CCTACGTTTT AACCATCTCC 
GATTCCAAGC CAGAGCACAC CTCGTATGCA TCGAATCTCT TGCTCCGTAA AAACCCGACT 
TCGTTACCAT TGGGCCAATA TCCGGAGGAT GTGAAGTTTG GCGATCCTCG AGAGATCTCA 
TTGCGGGTTG GTAACGGACC CACCTTGGCC TTTTCGGAGC AGGGTCTCCT TAAGTCCATT 
CAGCTTACTC AGGATAGCCC ACATGTACCG GTGCACTTCA AGTTCCTCAA GTATGGCGTT 
CGATCGCATG GCGATAGATC CGGTGCCTAT CTGTTCCTGC CCAATGGACC AGCTTCGCCA 
GTCGAGCTTG GCCAGCCAGT GGTCCTGGTG ACTAAGGGCA AACTGGAGTC GTCCGTGAGC 
GTGGGACTTC CGAGCGTGGT GCACCAGACG ATAATGCGCG GTGGTGCACC TGAGATTCGC 
AATCTGGTGG ATATAGGCTC ACTGGACAAC ACGGAGATCG TGATGCGCTT GGAGACGCAT 
ATCGACAGCG GCGATATCTT CTACACGGAT CTCAATGGAT TGCAATTTAT CAAGAGGCGG 
CGTTTGGACA AATTACCTTT GCAGGCCAAC TATTATCCCA TACCTTCTGG TATGTTCATT 
GAGGATGCCA ATACGCGACT CACTCTCCTC ACGGGTCAAC CGCTGGGTGG ATCTTCTCTG 
GCCTCGGGCG AGCTAGAGAT TATGCAAGAT CGTCGCCTGG CCAGCGATGA TGAACGCGGC 
CTGGGACAGG GTGTTTTGGA CAACAAGCCG GTGCTGCATA TTTATCGGCT GGTGCTGGAG 
AAGGTTAACA ACTGTGTCCG ACCGTCAGAG CTTCATCCTG CCGGCTATTT GACAAGTGCC 
GCTCACAAAG CATCGCAGTC ACTGCTGGAT CCACTGGACA AGTTTATATT CGCTGAAAAT 
GAGTGGATCG GGGCACAGGG GCAATTTGGT GGCGATCATC CTTCGGCTCG TGAGGATCTC 
GATGTGTCGG TGATGAGACG CTTAACCAAG AGCTCGGCCA AAACCCAGCG AGTAGGCTAC 
GTTCTGCACC GCACCAATCT GATGCAATGC GGCACTCCAG AGGAGCATAC ACAGAAGCTG 
GATGTGTGCC ACCTACTGCC GAATGTGGCG AGATGCGAGC GCACGACGCT GACTTTCCTG 
CAGAATTTGG AGCACTTGGA TGGCATGGTG GCGCCGGAAG TGTGCCCCAT GGAAACCGCC 
GCTTATGTGA GCAGTCACTC AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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alpha-Man-IIa cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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