man2a2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	man2a2
Entrez Gene ID	100333987
Full Name	mannosidase, alpha, class 2A, member 2
General protein information	Preferred Names mannosidase, alpha, class 2A, member 2 Names alpha-mannosidase 2x CG6206-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009297800.1	XP_009296075.1	alpha-mannosidase 2x isoform X1
XM_009297801.2	XP_009296076.1	alpha-mannosidase 2x isoform X1
XM_009297800.2	XP_009296075.1	alpha-mannosidase 2x isoform X1
XM_009297802.1	XP_009296077.1	alpha-mannosidase 2x isoform X2
XM_009297803.1	XP_009296078.1	alpha-mannosidase 2x isoform X3
XM_009297801.3	XP_009296076.1	alpha-mannosidase 2x isoform X1
XM_009297802.2	XP_009296077.1	alpha-mannosidase 2x isoform X2
XM_009297803.2	XP_009296078.1	alpha-mannosidase 2x isoform X3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Man2a2	NP_001100997.1
Oryza sativa (rice)	Os06g0245700	NP_001057277.2
Xenopus tropicalis (tropical clawed frog)	man2a2	NP_001006777.1
Pan troglodytes (chimpanzee)	MAN2A2	XP_001169951.1
Gallus gallus (chicken)	MAN2A2	XP_001233608.2
Danio rerio (zebrafish)	man2a2	XP_005163040.1
Homo sapiens (human)	MAN2A2	NP_006113.2
Bos taurus (cattle)	MAN2A2	NP_001179570.1
Mus musculus (house mouse)	Man2a2	NP_766491.2
Drosophila melanogaster (fruit fly)	alpha-Man-II	NP_524291.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004020	XP_318477.4
Canis lupus familiaris (dog)	MAN2A2	XP_545863.3
Arabidopsis thaliana (thale cress)	GMII	NP_196999.1
Caenorhabditis elegans (roundworm)	aman-2	NP_505995.2

Gene Ontology

The following man2a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the man2a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa49900	XM_009297800.2 Latest version!	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa49900	XM_009297801.3 Latest version!	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa49901	XM_009297802.2 Latest version!	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa49902	XM_009297803.2 Latest version!	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa49900	XM_009297800.1	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa49900	XM_009297801.2	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa49901	XM_009297802.1	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa49902	XM_009297803.1	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

man2a2 ( XM_009297800.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296075.1 Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49900
Clone ID Related Accession (Same CDS sequence)	XM_009297800.1 , XM_009297801.2 , XM_009297800.2 , XM_009297801.3
Accession Version	XM_009297800.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alpha-mannosidase 2x isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC 
CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT 
ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG 
CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT 
CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT 
GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG 
TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG 
ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG 
ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC 
AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT 
GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT 
TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG 
ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG 
CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG 
CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG 
AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC 
GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC 
AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT 
GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG 
GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG 
AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG 
TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG 
GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG 
GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT 
TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA 
CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG 
CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC 
GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT 
AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG 
CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT 
ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT 
CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA 
CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG 
GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG 
TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA 
CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA 
CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG 
CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC 
ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA 
AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG 
TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT 
CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT 
AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG 
GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC 
AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC 
CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG 
GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA 
CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT 
GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA 
GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC 
ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC 
ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC 
AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG 
CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC 
ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG 
CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT 
CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296075.1
CDS	788..4312
Translation	MKLKKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQNGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVLELTDTGAISPSGHLPFRSANGSWVLPFDGRPTFLSVKPLDCQFALGRRSQTDLQMLDVYSLLNFDNVDGGVWKQGFEITYEPNEWDDEPLQVFVVPHSHNDPGWIKTFDKYYNDQTQHILNNMVTKMAEDPRRKFIWSEISYFAKWWESADVHKQEVMRKLILSGQLEMVTGGWVMTDEANAHYFAMIDQLIEGHQWLERNLGVTPRSGWAVDPFGHSATMPYLLKRANISSMLIQRVHYSIKKHFAATRSLEFMWRQAWDIESSTDMFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGSRVNCPWKVPPRAINDANVAERAGVLLDQYRKKSKLFRSKVLLVPLGDDFRYDKALEWDQQYLNYQKLFDYMNSHPEMHVKAQFATLSDYFDAVYKANGVTSGARPPDYPVLSGDFFAYADREDHYWSGYYTSRPFYKNLDRILESHLRGAEILYSLAVAHARHAGVEGRYPVSDYSLLTDARRNIGLFQHHDAITGTAKEAVVIDYGNRLLRSLVGLKRVIINAAHFLIIKNKDIYRFYQTEPFLETDDRRATQDSLPQRTLIELESSPRYLVLFNPVEQERLCLVTVLVNSARVRVLTEDGQTLPVQLSAQWMSATEMSGEVYQASFMARLPALGLAVFHLYDSADSPMTLRSETLLRVPGRGQSIRGLDPLPVRSQTVDGQPFYIQSQSLTLGFSGATGLLESIRRKDDPQEVRVQIQFLTYGTRPSKDKSGAYLFLPDGNAKPYSQREAPIVRVVEGPLFAEVVAHYQHFQQTVRIHNVPGVDGLSLDITTMVDIRDQNNKELAMRFVTDIQSGDTFYTDLNGFQIQPRRYFQKLPLQANFYPMPSTAYIQDSQYRLTLHTAQALGVTSLASGQLEVILDRRLMQDDNRGLGQGLKDNKRTANRFRLLLERRSNSAKPAGSYANMLNKIIAGILGDGQEEPVDSRPVSFPSLLSHMTSTILNHEVLALPVLPRKHGVPPMRTFAPLAGTLPCDFHLLNLRSIQSPQDAHSPSPYMALLLHRLGLDCGLEPQNPGFNCTTTQGQLSVSGLFRGLDLQLLQPMSLSLMHSQPPLSNDSSVSLEPMEISAYKLKLC

Target ORF information:

RefSeq Version	XM_009297800.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297800.1

ORF Insert Sequence:

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC 
CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT 
ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG 
CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT 
CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT 
GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG 
TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG 
ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG 
ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC 
AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT 
GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT 
TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG 
ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG 
CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG 
CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG 
AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC 
GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC 
AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT 
GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG 
GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG 
AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG 
TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG 
GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG 
GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT 
TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA 
CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG 
CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC 
GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT 
AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG 
CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT 
ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT 
CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA 
CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG 
GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG 
TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA 
CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA 
CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG 
CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC 
ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA 
AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG 
TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT 
CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT 
AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG 
GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC 
AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC 
CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG 
GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA 
CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT 
GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA 
GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC 
ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC 
ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC 
AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG 
CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC 
ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG 
CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT 
CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_009297801.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296076.1 Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49900
Clone ID Related Accession (Same CDS sequence)	XM_009297800.1 , XM_009297801.2 , XM_009297800.2 , XM_009297801.3
Accession Version	XM_009297801.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alpha-mannosidase 2x isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297801.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC 
CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT 
ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG 
CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT 
CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT 
GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG 
TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG 
ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG 
ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC 
AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT 
GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT 
TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG 
ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG 
CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG 
CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG 
AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC 
GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC 
AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT 
GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG 
GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG 
AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG 
TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG 
GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG 
GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT 
TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA 
CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG 
CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC 
GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT 
AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG 
CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT 
ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT 
CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA 
CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG 
GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG 
TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA 
CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA 
CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG 
CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC 
ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA 
AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG 
TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT 
CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT 
AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG 
GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC 
AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC 
CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG 
GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA 
CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT 
GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA 
GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC 
ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC 
ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC 
AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG 
CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC 
ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG 
CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT 
CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296076.1
CDS	772..4296
Translation	MKLKKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQNGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVLELTDTGAISPSGHLPFRSANGSWVLPFDGRPTFLSVKPLDCQFALGRRSQTDLQMLDVYSLLNFDNVDGGVWKQGFEITYEPNEWDDEPLQVFVVPHSHNDPGWIKTFDKYYNDQTQHILNNMVTKMAEDPRRKFIWSEISYFAKWWESADVHKQEVMRKLILSGQLEMVTGGWVMTDEANAHYFAMIDQLIEGHQWLERNLGVTPRSGWAVDPFGHSATMPYLLKRANISSMLIQRVHYSIKKHFAATRSLEFMWRQAWDIESSTDMFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGSRVNCPWKVPPRAINDANVAERAGVLLDQYRKKSKLFRSKVLLVPLGDDFRYDKALEWDQQYLNYQKLFDYMNSHPEMHVKAQFATLSDYFDAVYKANGVTSGARPPDYPVLSGDFFAYADREDHYWSGYYTSRPFYKNLDRILESHLRGAEILYSLAVAHARHAGVEGRYPVSDYSLLTDARRNIGLFQHHDAITGTAKEAVVIDYGNRLLRSLVGLKRVIINAAHFLIIKNKDIYRFYQTEPFLETDDRRATQDSLPQRTLIELESSPRYLVLFNPVEQERLCLVTVLVNSARVRVLTEDGQTLPVQLSAQWMSATEMSGEVYQASFMARLPALGLAVFHLYDSADSPMTLRSETLLRVPGRGQSIRGLDPLPVRSQTVDGQPFYIQSQSLTLGFSGATGLLESIRRKDDPQEVRVQIQFLTYGTRPSKDKSGAYLFLPDGNAKPYSQREAPIVRVVEGPLFAEVVAHYQHFQQTVRIHNVPGVDGLSLDITTMVDIRDQNNKELAMRFVTDIQSGDTFYTDLNGFQIQPRRYFQKLPLQANFYPMPSTAYIQDSQYRLTLHTAQALGVTSLASGQLEVILDRRLMQDDNRGLGQGLKDNKRTANRFRLLLERRSNSAKPAGSYANMLNKIIAGILGDGQEEPVDSRPVSFPSLLSHMTSTILNHEVLALPVLPRKHGVPPMRTFAPLAGTLPCDFHLLNLRSIQSPQDAHSPSPYMALLLHRLGLDCGLEPQNPGFNCTTTQGQLSVSGLFRGLDLQLLQPMSLSLMHSQPPLSNDSSVSLEPMEISAYKLKLC

Target ORF information:

RefSeq Version	XM_009297801.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297801.2

ORF Insert Sequence:

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC 
CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT 
ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG 
CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT 
CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT 
GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG 
TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG 
ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG 
ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC 
AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT 
GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT 
TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG 
ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG 
CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG 
CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG 
AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC 
GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC 
AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT 
GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG 
GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG 
AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG 
TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG 
GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG 
GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT 
TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA 
CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG 
CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC 
GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT 
AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG 
CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT 
ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT 
CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA 
CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG 
GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG 
TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA 
CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA 
CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG 
CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC 
ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA 
AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG 
TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT 
CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT 
AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG 
GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC 
AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC 
CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG 
GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA 
CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT 
GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA 
GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC 
ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC 
ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC 
AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG 
CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC 
ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG 
CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT 
CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_009297800.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296075.1 Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA.

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CloneID	ODa49900
Clone ID Related Accession (Same CDS sequence)	XM_009297800.1 , XM_009297801.2 , XM_009297800.2 , XM_009297801.3
Accession Version	XM_009297800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC 
CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT 
ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG 
CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT 
CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT 
GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG 
TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG 
ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG 
ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC 
AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT 
GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT 
TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG 
ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG 
CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG 
CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG 
AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC 
GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC 
AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT 
GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG 
GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG 
AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG 
TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG 
GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG 
GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT 
TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA 
CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG 
CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC 
GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT 
AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG 
CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT 
ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT 
CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA 
CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG 
GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG 
TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA 
CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA 
CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG 
CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC 
ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA 
AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG 
TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT 
CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT 
AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG 
GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC 
AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC 
CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG 
GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA 
CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT 
GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA 
GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC 
ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC 
ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC 
AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG 
CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC 
ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG 
CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT 
CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296075.1
CDS	788..4312
Translation	MKLKKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQNGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVLELTDTGAISPSGHLPFRSANGSWVLPFDGRPTFLSVKPLDCQFALGRRSQTDLQMLDVYSLLNFDNVDGGVWKQGFEITYEPNEWDDEPLQVFVVPHSHNDPGWIKTFDKYYNDQTQHILNNMVTKMAEDPRRKFIWSEISYFAKWWESADVHKQEVMRKLILSGQLEMVTGGWVMTDEANAHYFAMIDQLIEGHQWLERNLGVTPRSGWAVDPFGHSATMPYLLKRANISSMLIQRVHYSIKKHFAATRSLEFMWRQAWDIESSTDMFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGSRVNCPWKVPPRAINDANVAERAGVLLDQYRKKSKLFRSKVLLVPLGDDFRYDKALEWDQQYLNYQKLFDYMNSHPEMHVKAQFATLSDYFDAVYKANGVTSGARPPDYPVLSGDFFAYADREDHYWSGYYTSRPFYKNLDRILESHLRGAEILYSLAVAHARHAGVEGRYPVSDYSLLTDARRNIGLFQHHDAITGTAKEAVVIDYGNRLLRSLVGLKRVIINAAHFLIIKNKDIYRFYQTEPFLETDDRRATQDSLPQRTLIELESSPRYLVLFNPVEQERLCLVTVLVNSARVRVLTEDGQTLPVQLSAQWMSATEMSGEVYQASFMARLPALGLAVFHLYDSADSPMTLRSETLLRVPGRGQSIRGLDPLPVRSQTVDGQPFYIQSQSLTLGFSGATGLLESIRRKDDPQEVRVQIQFLTYGTRPSKDKSGAYLFLPDGNAKPYSQREAPIVRVVEGPLFAEVVAHYQHFQQTVRIHNVPGVDGLSLDITTMVDIRDQNNKELAMRFVTDIQSGDTFYTDLNGFQIQPRRYFQKLPLQANFYPMPSTAYIQDSQYRLTLHTAQALGVTSLASGQLEVILDRRLMQDDNRGLGQGLKDNKRTANRFRLLLERRSNSAKPAGSYANMLNKIIAGILGDGQEEPVDSRPVSFPSLLSHMTSTILNHEVLALPVLPRKHGVPPMRTFAPLAGTLPCDFHLLNLRSIQSPQDAHSPSPYMALLLHRLGLDCGLEPQNPGFNCTTTQGQLSVSGLFRGLDLQLLQPMSLSLMHSQPPLSNDSSVSLEPMEISAYKLKLC

Target ORF information:

RefSeq Version	XM_009297800.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297800.2

ORF Insert Sequence:

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC 
CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT 
ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG 
CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT 
CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT 
GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG 
TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG 
ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG 
ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC 
AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT 
GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT 
TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG 
ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG 
CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG 
CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG 
AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC 
GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC 
AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT 
GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG 
GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG 
AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG 
TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG 
GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG 
GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT 
TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA 
CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG 
CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC 
GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT 
AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG 
CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT 
ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT 
CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA 
CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG 
GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG 
TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA 
CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA 
CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG 
CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC 
ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA 
AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG 
TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT 
CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT 
AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG 
GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC 
AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC 
CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG 
GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA 
CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT 
GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA 
GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC 
ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC 
ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC 
AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG 
CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC 
ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG 
CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT 
CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_009297801.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296076.1 Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA.

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CloneID	ODa49900
Clone ID Related Accession (Same CDS sequence)	XM_009297800.1 , XM_009297801.2 , XM_009297800.2 , XM_009297801.3
Accession Version	XM_009297801.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297801.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC 
CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT 
ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG 
CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT 
CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT 
GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG 
TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG 
ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG 
ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC 
AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT 
GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT 
TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG 
ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG 
CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG 
CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG 
AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC 
GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC 
AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT 
GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG 
GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG 
AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG 
TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG 
GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG 
GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT 
TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA 
CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG 
CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC 
GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT 
AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG 
CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT 
ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT 
CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA 
CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG 
GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG 
TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA 
CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA 
CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG 
CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC 
ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA 
AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG 
TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT 
CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT 
AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG 
GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC 
AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC 
CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG 
GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA 
CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT 
GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA 
GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC 
ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC 
ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC 
AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG 
CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC 
ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG 
CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT 
CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296076.1
CDS	780..4304
Translation	MKLKKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQNGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVLELTDTGAISPSGHLPFRSANGSWVLPFDGRPTFLSVKPLDCQFALGRRSQTDLQMLDVYSLLNFDNVDGGVWKQGFEITYEPNEWDDEPLQVFVVPHSHNDPGWIKTFDKYYNDQTQHILNNMVTKMAEDPRRKFIWSEISYFAKWWESADVHKQEVMRKLILSGQLEMVTGGWVMTDEANAHYFAMIDQLIEGHQWLERNLGVTPRSGWAVDPFGHSATMPYLLKRANISSMLIQRVHYSIKKHFAATRSLEFMWRQAWDIESSTDMFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGSRVNCPWKVPPRAINDANVAERAGVLLDQYRKKSKLFRSKVLLVPLGDDFRYDKALEWDQQYLNYQKLFDYMNSHPEMHVKAQFATLSDYFDAVYKANGVTSGARPPDYPVLSGDFFAYADREDHYWSGYYTSRPFYKNLDRILESHLRGAEILYSLAVAHARHAGVEGRYPVSDYSLLTDARRNIGLFQHHDAITGTAKEAVVIDYGNRLLRSLVGLKRVIINAAHFLIIKNKDIYRFYQTEPFLETDDRRATQDSLPQRTLIELESSPRYLVLFNPVEQERLCLVTVLVNSARVRVLTEDGQTLPVQLSAQWMSATEMSGEVYQASFMARLPALGLAVFHLYDSADSPMTLRSETLLRVPGRGQSIRGLDPLPVRSQTVDGQPFYIQSQSLTLGFSGATGLLESIRRKDDPQEVRVQIQFLTYGTRPSKDKSGAYLFLPDGNAKPYSQREAPIVRVVEGPLFAEVVAHYQHFQQTVRIHNVPGVDGLSLDITTMVDIRDQNNKELAMRFVTDIQSGDTFYTDLNGFQIQPRRYFQKLPLQANFYPMPSTAYIQDSQYRLTLHTAQALGVTSLASGQLEVILDRRLMQDDNRGLGQGLKDNKRTANRFRLLLERRSNSAKPAGSYANMLNKIIAGILGDGQEEPVDSRPVSFPSLLSHMTSTILNHEVLALPVLPRKHGVPPMRTFAPLAGTLPCDFHLLNLRSIQSPQDAHSPSPYMALLLHRLGLDCGLEPQNPGFNCTTTQGQLSVSGLFRGLDLQLLQPMSLSLMHSQPPLSNDSSVSLEPMEISAYKLKLC

Target ORF information:

RefSeq Version	XM_009297801.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297801.3

ORF Insert Sequence:

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC 
CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT 
ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG 
CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT 
CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT 
GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG 
TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG 
ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG 
ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC 
AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT 
GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT 
TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG 
ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG 
CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG 
CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG 
AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC 
GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC 
AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT 
GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG 
GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG 
AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG 
TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG 
GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG 
GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT 
TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA 
CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG 
CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC 
GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT 
AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG 
CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT 
ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT 
CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA 
CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG 
GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG 
TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA 
CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA 
CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG 
CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC 
ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA 
AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG 
TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT 
CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT 
AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG 
GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC 
AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC 
CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG 
GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA 
CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT 
GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA 
GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC 
ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC 
ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC 
AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG 
CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC 
ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG 
CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT 
CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_009297802.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296077.1 Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA.

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CloneID	ODa49901
Clone ID Related Accession (Same CDS sequence)	XM_009297802.1 , XM_009297802.2
Accession Version	XM_009297802.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alpha-mannosidase 2x isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAACCGCAT CGAGCAGCTG GAACAGCTCC TGGAGGAGAA CCATCAGATC 
ATCAGCCACA TTAAAGACTC AGTGCTAGAG CTCACTGATA CGGGCGCCAT CTCTCCCAGT 
GGACACCTGC CCTTCCGGAG CGCCAACGGC TCCTGGGTGC TGCCGTTTGA TGGACGGCCC 
ACGTTTCTCT CAGTCAAGCC ATTGGACTGC CAGTTTGCTC TGGGCCGCAG GAGCCAAACT 
GACCTGCAGA TGCTGGATGT TTATTCCTTG CTGAACTTTG ATAATGTGGA CGGAGGGGTC 
TGGAAGCAAG GCTTTGAGAT CACCTATGAG CCCAACGAGT GGGACGATGA GCCCTTGCAG 
GTGTTTGTGG TTCCGCATTC GCACAACGAT CCAGGATGGA TCAAAACGTT TGACAAGTAC 
TACAACGACC AGACCCAGCA CATCTTAAAC AACATGGTGA CGAAGATGGC AGAAGATCCT 
CGCCGGAAGT TCATCTGGTC GGAAATATCG TACTTCGCCA AATGGTGGGA AAGCGCAGAT 
GTGCATAAAC AAGAGGTTAT GCGCAAGCTA ATCCTCAGTG GACAGCTGGA GATGGTAACC 
GGAGGCTGGG TGATGACGGA CGAAGCCAAC GCTCACTATT TCGCCATGAT CGATCAGCTC 
ATTGAAGGCC ATCAGTGGCT GGAGAGGAAT CTGGGTGTGA CCCCTCGCTC GGGCTGGGCT 
GTTGATCCAT TCGGGCACAG CGCCACCATG CCTTACCTGC TGAAACGGGC CAACATAAGC 
AGCATGTTGA TCCAGAGGGT CCATTACTCC ATCAAGAAGC ACTTCGCAGC CACACGCAGC 
CTTGAGTTCA TGTGGAGACA GGCCTGGGAT ATCGAGTCGA GCACAGACAT GTTCTGCCAC 
ATGATGCCGT TTTACAGCTA TGATGTGCCT CACACGTGCG GCCCCGACCC CAAAATCTGC 
TGCCAGTTTG ATTTCAAGAG GCTGCCGGGC AGTCGAGTCA ACTGCCCGTG GAAGGTGCCG 
CCCAGAGCTA TCAATGATGC CAATGTGGCC GAGAGAGCTG GCGTTCTGCT CGATCAATAT 
CGTAAAAAAT CGAAGCTGTT CCGGAGCAAA GTGTTGCTGG TTCCTCTGGG GGACGACTTT 
CGCTATGATA AAGCTTTAGA GTGGGACCAG CAGTACTTGA ACTACCAGAA ACTGTTCGAT 
TACATGAACT CCCATCCAGA AATGCACGTG AAGGCTCAGT TTGCGACGCT GTCAGACTAC 
TTTGATGCGG TGTACAAGGC TAATGGAGTG ACTTCGGGGG CGAGACCACC CGATTACCCT 
GTGCTGAGCG GAGACTTCTT CGCTTATGCA GACAGAGAGG ACCATTACTG GAGTGGATAC 
TACACCTCGC GACCCTTCTA CAAAAACCTG GACCGCATTT TAGAATCTCA CCTAAGAGGG 
GCAGAAATCC TCTATAGTTT GGCGGTGGCT CATGCACGAC ATGCAGGAGT TGAAGGACGA 
TATCCGGTTT CAGATTACTC TCTCCTGACT GATGCCAGGC GAAACATTGG GCTGTTCCAG 
CATCATGATG CCATCACGGG CACTGCCAAA GAGGCTGTCG TCATCGACTA CGGGAACAGG 
CTGTTACGTT CTCTGGTGGG TCTGAAACGA GTGATCATTA ATGCAGCGCA CTTCCTGATA 
ATAAAGAATA AAGACATTTA TCGCTTCTAC CAGACAGAGC CCTTCTTGGA GACGGATGAT 
AGACGCGCCA CACAGGACTC TCTCCCGCAG CGTACGCTTA TCGAGCTGGA ATCTTCACCA 
AGGTATCTGG TGCTGTTTAA CCCGGTGGAG CAGGAGCGTC TGTGTTTGGT CACTGTCCTG 
GTGAATTCGG CAAGAGTGAG GGTGCTTACA GAAGACGGAC AAACGCTACC GGTGCAGCTC 
AGCGCGCAGT GGATGTCCGC TACGGAGATG AGTGGGGAGG TTTATCAGGC TTCTTTCATG 
GCACGTCTGC CGGCTCTGGG ATTGGCCGTC TTCCACCTGT ACGACTCTGC TGATTCGCCC 
ATGACTCTGC GCTCCGAGAC GCTGCTCAGG GTTCCAGGAC GGGGTCAGAG CATTCGGGGT 
TTGGATCCTC TGCCTGTGCG CTCACAAACT GTTGATGGAC AGCCGTTCTA CATCCAAAGC 
CAATCCCTCA CTCTGGGCTT CTCTGGAGCC ACAGGGCTGC TGGAGAGCAT TCGACGGAAA 
GACGATCCCC AGGAGGTGAG GGTACAGATT CAGTTCCTCA CCTACGGTAC GCGACCCTCC 
AAGGACAAGA GCGGGGCCTA TCTGTTCCTG CCGGATGGAA ATGCTAAGCC GTATTCGCAG 
AGAGAGGCCC CTATCGTGCG TGTGGTTGAA GGTCCTCTGT TCGCAGAGGT TGTGGCACAT 
TATCAGCACT TCCAGCAGAC GGTCCGCATT CATAATGTTC CAGGGGTTGA CGGTCTTTCT 
TTGGACATCA CCACTATGGT GGACATCAGA GATCAGAATA ACAAAGAGCT GGCCATGAGG 
TTCGTCACCG ACATCCAGAG CGGAGATACT TTCTACACGG ATCTCAACGG CTTTCAGATT 
CAGCCTCGGC GATACTTCCA GAAACTTCCA CTACAAGCCA ATTTCTACCC CATGCCCAGC 
ACAGCATACA TCCAGGACAG CCAGTACCGG CTCACGCTCC ACACGGCACA GGCTCTGGGG 
GTTACCAGTC TGGCCAGCGG TCAGCTGGAG GTAATCCTGG ACCGCAGACT GATGCAGGAT 
GATAACAGGG GATTGGGACA AGGCCTGAAA GACAACAAAC GGACAGCTAA CAGGTTCCGT 
CTTCTGCTGG AGAGGCGGAG CAACAGTGCA AAGCCTGCTG GCTCCTATGC CAACATGCTA 
AACAAAATCA TTGCTGGTAT TTTAGGAGAC GGCCAGGAAG AGCCGGTGGA CAGCAGACCG 
GTCAGCTTCC CGTCTCTGCT CAGTCACATG ACCTCCACCA TCCTCAACCA TGAGGTGCTG 
GCACTGCCCG TGTTGCCCAG GAAACATGGC GTGCCACCCA TGCGAACCTT CGCCCCATTG 
GCTGGCACTC TGCCCTGCGA CTTCCACCTG CTGAACCTCA GGAGCATCCA AAGCCCGCAG 
GACGCTCACT CTCCATCTCC CTATATGGCT TTGCTGCTGC ACAGACTGGG TTTGGACTGT 
GGTCTGGAGC CGCAGAATCC TGGTTTCAAC TGCACCACCA CACAAGGCCA GCTCTCCGTT 
TCTGGGCTGT TCCGTGGGCT GGATCTCCAG CTCCTGCAGC CCATGTCTCT GTCTCTGATG 
CACTCTCAGC CTCCGCTCTC TAACGACTCC TCCGTCAGTC TGGAGCCAAT GGAGATCTCC 
GCCTACAAGC TCAAGCTGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296077.1
CDS	788..4291
Translation	MKLKKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQNGGNFPRNRIEQLEQLLEENHQIISHIKDSVLELTDTGAISPSGHLPFRSANGSWVLPFDGRPTFLSVKPLDCQFALGRRSQTDLQMLDVYSLLNFDNVDGGVWKQGFEITYEPNEWDDEPLQVFVVPHSHNDPGWIKTFDKYYNDQTQHILNNMVTKMAEDPRRKFIWSEISYFAKWWESADVHKQEVMRKLILSGQLEMVTGGWVMTDEANAHYFAMIDQLIEGHQWLERNLGVTPRSGWAVDPFGHSATMPYLLKRANISSMLIQRVHYSIKKHFAATRSLEFMWRQAWDIESSTDMFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGSRVNCPWKVPPRAINDANVAERAGVLLDQYRKKSKLFRSKVLLVPLGDDFRYDKALEWDQQYLNYQKLFDYMNSHPEMHVKAQFATLSDYFDAVYKANGVTSGARPPDYPVLSGDFFAYADREDHYWSGYYTSRPFYKNLDRILESHLRGAEILYSLAVAHARHAGVEGRYPVSDYSLLTDARRNIGLFQHHDAITGTAKEAVVIDYGNRLLRSLVGLKRVIINAAHFLIIKNKDIYRFYQTEPFLETDDRRATQDSLPQRTLIELESSPRYLVLFNPVEQERLCLVTVLVNSARVRVLTEDGQTLPVQLSAQWMSATEMSGEVYQASFMARLPALGLAVFHLYDSADSPMTLRSETLLRVPGRGQSIRGLDPLPVRSQTVDGQPFYIQSQSLTLGFSGATGLLESIRRKDDPQEVRVQIQFLTYGTRPSKDKSGAYLFLPDGNAKPYSQREAPIVRVVEGPLFAEVVAHYQHFQQTVRIHNVPGVDGLSLDITTMVDIRDQNNKELAMRFVTDIQSGDTFYTDLNGFQIQPRRYFQKLPLQANFYPMPSTAYIQDSQYRLTLHTAQALGVTSLASGQLEVILDRRLMQDDNRGLGQGLKDNKRTANRFRLLLERRSNSAKPAGSYANMLNKIIAGILGDGQEEPVDSRPVSFPSLLSHMTSTILNHEVLALPVLPRKHGVPPMRTFAPLAGTLPCDFHLLNLRSIQSPQDAHSPSPYMALLLHRLGLDCGLEPQNPGFNCTTTQGQLSVSGLFRGLDLQLLQPMSLSLMHSQPPLSNDSSVSLEPMEISAYKLKLC

Target ORF information:

RefSeq Version	XM_009297802.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297802.1

ORF Insert Sequence:

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAACCGCAT CGAGCAGCTG GAACAGCTCC TGGAGGAGAA CCATCAGATC 
ATCAGCCACA TTAAAGACTC AGTGCTAGAG CTCACTGATA CGGGCGCCAT CTCTCCCAGT 
GGACACCTGC CCTTCCGGAG CGCCAACGGC TCCTGGGTGC TGCCGTTTGA TGGACGGCCC 
ACGTTTCTCT CAGTCAAGCC ATTGGACTGC CAGTTTGCTC TGGGCCGCAG GAGCCAAACT 
GACCTGCAGA TGCTGGATGT TTATTCCTTG CTGAACTTTG ATAATGTGGA CGGAGGGGTC 
TGGAAGCAAG GCTTTGAGAT CACCTATGAG CCCAACGAGT GGGACGATGA GCCCTTGCAG 
GTGTTTGTGG TTCCGCATTC GCACAACGAT CCAGGATGGA TCAAAACGTT TGACAAGTAC 
TACAACGACC AGACCCAGCA CATCTTAAAC AACATGGTGA CGAAGATGGC AGAAGATCCT 
CGCCGGAAGT TCATCTGGTC GGAAATATCG TACTTCGCCA AATGGTGGGA AAGCGCAGAT 
GTGCATAAAC AAGAGGTTAT GCGCAAGCTA ATCCTCAGTG GACAGCTGGA GATGGTAACC 
GGAGGCTGGG TGATGACGGA CGAAGCCAAC GCTCACTATT TCGCCATGAT CGATCAGCTC 
ATTGAAGGCC ATCAGTGGCT GGAGAGGAAT CTGGGTGTGA CCCCTCGCTC GGGCTGGGCT 
GTTGATCCAT TCGGGCACAG CGCCACCATG CCTTACCTGC TGAAACGGGC CAACATAAGC 
AGCATGTTGA TCCAGAGGGT CCATTACTCC ATCAAGAAGC ACTTCGCAGC CACACGCAGC 
CTTGAGTTCA TGTGGAGACA GGCCTGGGAT ATCGAGTCGA GCACAGACAT GTTCTGCCAC 
ATGATGCCGT TTTACAGCTA TGATGTGCCT CACACGTGCG GCCCCGACCC CAAAATCTGC 
TGCCAGTTTG ATTTCAAGAG GCTGCCGGGC AGTCGAGTCA ACTGCCCGTG GAAGGTGCCG 
CCCAGAGCTA TCAATGATGC CAATGTGGCC GAGAGAGCTG GCGTTCTGCT CGATCAATAT 
CGTAAAAAAT CGAAGCTGTT CCGGAGCAAA GTGTTGCTGG TTCCTCTGGG GGACGACTTT 
CGCTATGATA AAGCTTTAGA GTGGGACCAG CAGTACTTGA ACTACCAGAA ACTGTTCGAT 
TACATGAACT CCCATCCAGA AATGCACGTG AAGGCTCAGT TTGCGACGCT GTCAGACTAC 
TTTGATGCGG TGTACAAGGC TAATGGAGTG ACTTCGGGGG CGAGACCACC CGATTACCCT 
GTGCTGAGCG GAGACTTCTT CGCTTATGCA GACAGAGAGG ACCATTACTG GAGTGGATAC 
TACACCTCGC GACCCTTCTA CAAAAACCTG GACCGCATTT TAGAATCTCA CCTAAGAGGG 
GCAGAAATCC TCTATAGTTT GGCGGTGGCT CATGCACGAC ATGCAGGAGT TGAAGGACGA 
TATCCGGTTT CAGATTACTC TCTCCTGACT GATGCCAGGC GAAACATTGG GCTGTTCCAG 
CATCATGATG CCATCACGGG CACTGCCAAA GAGGCTGTCG TCATCGACTA CGGGAACAGG 
CTGTTACGTT CTCTGGTGGG TCTGAAACGA GTGATCATTA ATGCAGCGCA CTTCCTGATA 
ATAAAGAATA AAGACATTTA TCGCTTCTAC CAGACAGAGC CCTTCTTGGA GACGGATGAT 
AGACGCGCCA CACAGGACTC TCTCCCGCAG CGTACGCTTA TCGAGCTGGA ATCTTCACCA 
AGGTATCTGG TGCTGTTTAA CCCGGTGGAG CAGGAGCGTC TGTGTTTGGT CACTGTCCTG 
GTGAATTCGG CAAGAGTGAG GGTGCTTACA GAAGACGGAC AAACGCTACC GGTGCAGCTC 
AGCGCGCAGT GGATGTCCGC TACGGAGATG AGTGGGGAGG TTTATCAGGC TTCTTTCATG 
GCACGTCTGC CGGCTCTGGG ATTGGCCGTC TTCCACCTGT ACGACTCTGC TGATTCGCCC 
ATGACTCTGC GCTCCGAGAC GCTGCTCAGG GTTCCAGGAC GGGGTCAGAG CATTCGGGGT 
TTGGATCCTC TGCCTGTGCG CTCACAAACT GTTGATGGAC AGCCGTTCTA CATCCAAAGC 
CAATCCCTCA CTCTGGGCTT CTCTGGAGCC ACAGGGCTGC TGGAGAGCAT TCGACGGAAA 
GACGATCCCC AGGAGGTGAG GGTACAGATT CAGTTCCTCA CCTACGGTAC GCGACCCTCC 
AAGGACAAGA GCGGGGCCTA TCTGTTCCTG CCGGATGGAA ATGCTAAGCC GTATTCGCAG 
AGAGAGGCCC CTATCGTGCG TGTGGTTGAA GGTCCTCTGT TCGCAGAGGT TGTGGCACAT 
TATCAGCACT TCCAGCAGAC GGTCCGCATT CATAATGTTC CAGGGGTTGA CGGTCTTTCT 
TTGGACATCA CCACTATGGT GGACATCAGA GATCAGAATA ACAAAGAGCT GGCCATGAGG 
TTCGTCACCG ACATCCAGAG CGGAGATACT TTCTACACGG ATCTCAACGG CTTTCAGATT 
CAGCCTCGGC GATACTTCCA GAAACTTCCA CTACAAGCCA ATTTCTACCC CATGCCCAGC 
ACAGCATACA TCCAGGACAG CCAGTACCGG CTCACGCTCC ACACGGCACA GGCTCTGGGG 
GTTACCAGTC TGGCCAGCGG TCAGCTGGAG GTAATCCTGG ACCGCAGACT GATGCAGGAT 
GATAACAGGG GATTGGGACA AGGCCTGAAA GACAACAAAC GGACAGCTAA CAGGTTCCGT 
CTTCTGCTGG AGAGGCGGAG CAACAGTGCA AAGCCTGCTG GCTCCTATGC CAACATGCTA 
AACAAAATCA TTGCTGGTAT TTTAGGAGAC GGCCAGGAAG AGCCGGTGGA CAGCAGACCG 
GTCAGCTTCC CGTCTCTGCT CAGTCACATG ACCTCCACCA TCCTCAACCA TGAGGTGCTG 
GCACTGCCCG TGTTGCCCAG GAAACATGGC GTGCCACCCA TGCGAACCTT CGCCCCATTG 
GCTGGCACTC TGCCCTGCGA CTTCCACCTG CTGAACCTCA GGAGCATCCA AAGCCCGCAG 
GACGCTCACT CTCCATCTCC CTATATGGCT TTGCTGCTGC ACAGACTGGG TTTGGACTGT 
GGTCTGGAGC CGCAGAATCC TGGTTTCAAC TGCACCACCA CACAAGGCCA GCTCTCCGTT 
TCTGGGCTGT TCCGTGGGCT GGATCTCCAG CTCCTGCAGC CCATGTCTCT GTCTCTGATG 
CACTCTCAGC CTCCGCTCTC TAACGACTCC TCCGTCAGTC TGGAGCCAAT GGAGATCTCC 
GCCTACAAGC TCAAGCTGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_009297802.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296077.1 Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA.

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CloneID	ODa49901
Clone ID Related Accession (Same CDS sequence)	XM_009297802.1 , XM_009297802.2
Accession Version	XM_009297802.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-mannosidase 2x isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAACCGCAT CGAGCAGCTG GAACAGCTCC TGGAGGAGAA CCATCAGATC 
ATCAGCCACA TTAAAGACTC AGTGCTAGAG CTCACTGATA CGGGCGCCAT CTCTCCCAGT 
GGACACCTGC CCTTCCGGAG CGCCAACGGC TCCTGGGTGC TGCCGTTTGA TGGACGGCCC 
ACGTTTCTCT CAGTCAAGCC ATTGGACTGC CAGTTTGCTC TGGGCCGCAG GAGCCAAACT 
GACCTGCAGA TGCTGGATGT TTATTCCTTG CTGAACTTTG ATAATGTGGA CGGAGGGGTC 
TGGAAGCAAG GCTTTGAGAT CACCTATGAG CCCAACGAGT GGGACGATGA GCCCTTGCAG 
GTGTTTGTGG TTCCGCATTC GCACAACGAT CCAGGATGGA TCAAAACGTT TGACAAGTAC 
TACAACGACC AGACCCAGCA CATCTTAAAC AACATGGTGA CGAAGATGGC AGAAGATCCT 
CGCCGGAAGT TCATCTGGTC GGAAATATCG TACTTCGCCA AATGGTGGGA AAGCGCAGAT 
GTGCATAAAC AAGAGGTTAT GCGCAAGCTA ATCCTCAGTG GACAGCTGGA GATGGTAACC 
GGAGGCTGGG TGATGACGGA CGAAGCCAAC GCTCACTATT TCGCCATGAT CGATCAGCTC 
ATTGAAGGCC ATCAGTGGCT GGAGAGGAAT CTGGGTGTGA CCCCTCGCTC GGGCTGGGCT 
GTTGATCCAT TCGGGCACAG CGCCACCATG CCTTACCTGC TGAAACGGGC CAACATAAGC 
AGCATGTTGA TCCAGAGGGT CCATTACTCC ATCAAGAAGC ACTTCGCAGC CACACGCAGC 
CTTGAGTTCA TGTGGAGACA GGCCTGGGAT ATCGAGTCGA GCACAGACAT GTTCTGCCAC 
ATGATGCCGT TTTACAGCTA TGATGTGCCT CACACGTGCG GCCCCGACCC CAAAATCTGC 
TGCCAGTTTG ATTTCAAGAG GCTGCCGGGC AGTCGAGTCA ACTGCCCGTG GAAGGTGCCG 
CCCAGAGCTA TCAATGATGC CAATGTGGCC GAGAGAGCTG GCGTTCTGCT CGATCAATAT 
CGTAAAAAAT CGAAGCTGTT CCGGAGCAAA GTGTTGCTGG TTCCTCTGGG GGACGACTTT 
CGCTATGATA AAGCTTTAGA GTGGGACCAG CAGTACTTGA ACTACCAGAA ACTGTTCGAT 
TACATGAACT CCCATCCAGA AATGCACGTG AAGGCTCAGT TTGCGACGCT GTCAGACTAC 
TTTGATGCGG TGTACAAGGC TAATGGAGTG ACTTCGGGGG CGAGACCACC CGATTACCCT 
GTGCTGAGCG GAGACTTCTT CGCTTATGCA GACAGAGAGG ACCATTACTG GAGTGGATAC 
TACACCTCGC GACCCTTCTA CAAAAACCTG GACCGCATTT TAGAATCTCA CCTAAGAGGG 
GCAGAAATCC TCTATAGTTT GGCGGTGGCT CATGCACGAC ATGCAGGAGT TGAAGGACGA 
TATCCGGTTT CAGATTACTC TCTCCTGACT GATGCCAGGC GAAACATTGG GCTGTTCCAG 
CATCATGATG CCATCACGGG CACTGCCAAA GAGGCTGTCG TCATCGACTA CGGGAACAGG 
CTGTTACGTT CTCTGGTGGG TCTGAAACGA GTGATCATTA ATGCAGCGCA CTTCCTGATA 
ATAAAGAATA AAGACATTTA TCGCTTCTAC CAGACAGAGC CCTTCTTGGA GACGGATGAT 
AGACGCGCCA CACAGGACTC TCTCCCGCAG CGTACGCTTA TCGAGCTGGA ATCTTCACCA 
AGGTATCTGG TGCTGTTTAA CCCGGTGGAG CAGGAGCGTC TGTGTTTGGT CACTGTCCTG 
GTGAATTCGG CAAGAGTGAG GGTGCTTACA GAAGACGGAC AAACGCTACC GGTGCAGCTC 
AGCGCGCAGT GGATGTCCGC TACGGAGATG AGTGGGGAGG TTTATCAGGC TTCTTTCATG 
GCACGTCTGC CGGCTCTGGG ATTGGCCGTC TTCCACCTGT ACGACTCTGC TGATTCGCCC 
ATGACTCTGC GCTCCGAGAC GCTGCTCAGG GTTCCAGGAC GGGGTCAGAG CATTCGGGGT 
TTGGATCCTC TGCCTGTGCG CTCACAAACT GTTGATGGAC AGCCGTTCTA CATCCAAAGC 
CAATCCCTCA CTCTGGGCTT CTCTGGAGCC ACAGGGCTGC TGGAGAGCAT TCGACGGAAA 
GACGATCCCC AGGAGGTGAG GGTACAGATT CAGTTCCTCA CCTACGGTAC GCGACCCTCC 
AAGGACAAGA GCGGGGCCTA TCTGTTCCTG CCGGATGGAA ATGCTAAGCC GTATTCGCAG 
AGAGAGGCCC CTATCGTGCG TGTGGTTGAA GGTCCTCTGT TCGCAGAGGT TGTGGCACAT 
TATCAGCACT TCCAGCAGAC GGTCCGCATT CATAATGTTC CAGGGGTTGA CGGTCTTTCT 
TTGGACATCA CCACTATGGT GGACATCAGA GATCAGAATA ACAAAGAGCT GGCCATGAGG 
TTCGTCACCG ACATCCAGAG CGGAGATACT TTCTACACGG ATCTCAACGG CTTTCAGATT 
CAGCCTCGGC GATACTTCCA GAAACTTCCA CTACAAGCCA ATTTCTACCC CATGCCCAGC 
ACAGCATACA TCCAGGACAG CCAGTACCGG CTCACGCTCC ACACGGCACA GGCTCTGGGG 
GTTACCAGTC TGGCCAGCGG TCAGCTGGAG GTAATCCTGG ACCGCAGACT GATGCAGGAT 
GATAACAGGG GATTGGGACA AGGCCTGAAA GACAACAAAC GGACAGCTAA CAGGTTCCGT 
CTTCTGCTGG AGAGGCGGAG CAACAGTGCA AAGCCTGCTG GCTCCTATGC CAACATGCTA 
AACAAAATCA TTGCTGGTAT TTTAGGAGAC GGCCAGGAAG AGCCGGTGGA CAGCAGACCG 
GTCAGCTTCC CGTCTCTGCT CAGTCACATG ACCTCCACCA TCCTCAACCA TGAGGTGCTG 
GCACTGCCCG TGTTGCCCAG GAAACATGGC GTGCCACCCA TGCGAACCTT CGCCCCATTG 
GCTGGCACTC TGCCCTGCGA CTTCCACCTG CTGAACCTCA GGAGCATCCA AAGCCCGCAG 
GACGCTCACT CTCCATCTCC CTATATGGCT TTGCTGCTGC ACAGACTGGG TTTGGACTGT 
GGTCTGGAGC CGCAGAATCC TGGTTTCAAC TGCACCACCA CACAAGGCCA GCTCTCCGTT 
TCTGGGCTGT TCCGTGGGCT GGATCTCCAG CTCCTGCAGC CCATGTCTCT GTCTCTGATG 
CACTCTCAGC CTCCGCTCTC TAACGACTCC TCCGTCAGTC TGGAGCCAAT GGAGATCTCC 
GCCTACAAGC TCAAGCTGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296077.1
CDS	788..4291
Translation	MKLKKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQNGGNFPRNRIEQLEQLLEENHQIISHIKDSVLELTDTGAISPSGHLPFRSANGSWVLPFDGRPTFLSVKPLDCQFALGRRSQTDLQMLDVYSLLNFDNVDGGVWKQGFEITYEPNEWDDEPLQVFVVPHSHNDPGWIKTFDKYYNDQTQHILNNMVTKMAEDPRRKFIWSEISYFAKWWESADVHKQEVMRKLILSGQLEMVTGGWVMTDEANAHYFAMIDQLIEGHQWLERNLGVTPRSGWAVDPFGHSATMPYLLKRANISSMLIQRVHYSIKKHFAATRSLEFMWRQAWDIESSTDMFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGSRVNCPWKVPPRAINDANVAERAGVLLDQYRKKSKLFRSKVLLVPLGDDFRYDKALEWDQQYLNYQKLFDYMNSHPEMHVKAQFATLSDYFDAVYKANGVTSGARPPDYPVLSGDFFAYADREDHYWSGYYTSRPFYKNLDRILESHLRGAEILYSLAVAHARHAGVEGRYPVSDYSLLTDARRNIGLFQHHDAITGTAKEAVVIDYGNRLLRSLVGLKRVIINAAHFLIIKNKDIYRFYQTEPFLETDDRRATQDSLPQRTLIELESSPRYLVLFNPVEQERLCLVTVLVNSARVRVLTEDGQTLPVQLSAQWMSATEMSGEVYQASFMARLPALGLAVFHLYDSADSPMTLRSETLLRVPGRGQSIRGLDPLPVRSQTVDGQPFYIQSQSLTLGFSGATGLLESIRRKDDPQEVRVQIQFLTYGTRPSKDKSGAYLFLPDGNAKPYSQREAPIVRVVEGPLFAEVVAHYQHFQQTVRIHNVPGVDGLSLDITTMVDIRDQNNKELAMRFVTDIQSGDTFYTDLNGFQIQPRRYFQKLPLQANFYPMPSTAYIQDSQYRLTLHTAQALGVTSLASGQLEVILDRRLMQDDNRGLGQGLKDNKRTANRFRLLLERRSNSAKPAGSYANMLNKIIAGILGDGQEEPVDSRPVSFPSLLSHMTSTILNHEVLALPVLPRKHGVPPMRTFAPLAGTLPCDFHLLNLRSIQSPQDAHSPSPYMALLLHRLGLDCGLEPQNPGFNCTTTQGQLSVSGLFRGLDLQLLQPMSLSLMHSQPPLSNDSSVSLEPMEISAYKLKLC

Target ORF information:

RefSeq Version	XM_009297802.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297802.2

ORF Insert Sequence:

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAACCGCAT CGAGCAGCTG GAACAGCTCC TGGAGGAGAA CCATCAGATC 
ATCAGCCACA TTAAAGACTC AGTGCTAGAG CTCACTGATA CGGGCGCCAT CTCTCCCAGT 
GGACACCTGC CCTTCCGGAG CGCCAACGGC TCCTGGGTGC TGCCGTTTGA TGGACGGCCC 
ACGTTTCTCT CAGTCAAGCC ATTGGACTGC CAGTTTGCTC TGGGCCGCAG GAGCCAAACT 
GACCTGCAGA TGCTGGATGT TTATTCCTTG CTGAACTTTG ATAATGTGGA CGGAGGGGTC 
TGGAAGCAAG GCTTTGAGAT CACCTATGAG CCCAACGAGT GGGACGATGA GCCCTTGCAG 
GTGTTTGTGG TTCCGCATTC GCACAACGAT CCAGGATGGA TCAAAACGTT TGACAAGTAC 
TACAACGACC AGACCCAGCA CATCTTAAAC AACATGGTGA CGAAGATGGC AGAAGATCCT 
CGCCGGAAGT TCATCTGGTC GGAAATATCG TACTTCGCCA AATGGTGGGA AAGCGCAGAT 
GTGCATAAAC AAGAGGTTAT GCGCAAGCTA ATCCTCAGTG GACAGCTGGA GATGGTAACC 
GGAGGCTGGG TGATGACGGA CGAAGCCAAC GCTCACTATT TCGCCATGAT CGATCAGCTC 
ATTGAAGGCC ATCAGTGGCT GGAGAGGAAT CTGGGTGTGA CCCCTCGCTC GGGCTGGGCT 
GTTGATCCAT TCGGGCACAG CGCCACCATG CCTTACCTGC TGAAACGGGC CAACATAAGC 
AGCATGTTGA TCCAGAGGGT CCATTACTCC ATCAAGAAGC ACTTCGCAGC CACACGCAGC 
CTTGAGTTCA TGTGGAGACA GGCCTGGGAT ATCGAGTCGA GCACAGACAT GTTCTGCCAC 
ATGATGCCGT TTTACAGCTA TGATGTGCCT CACACGTGCG GCCCCGACCC CAAAATCTGC 
TGCCAGTTTG ATTTCAAGAG GCTGCCGGGC AGTCGAGTCA ACTGCCCGTG GAAGGTGCCG 
CCCAGAGCTA TCAATGATGC CAATGTGGCC GAGAGAGCTG GCGTTCTGCT CGATCAATAT 
CGTAAAAAAT CGAAGCTGTT CCGGAGCAAA GTGTTGCTGG TTCCTCTGGG GGACGACTTT 
CGCTATGATA AAGCTTTAGA GTGGGACCAG CAGTACTTGA ACTACCAGAA ACTGTTCGAT 
TACATGAACT CCCATCCAGA AATGCACGTG AAGGCTCAGT TTGCGACGCT GTCAGACTAC 
TTTGATGCGG TGTACAAGGC TAATGGAGTG ACTTCGGGGG CGAGACCACC CGATTACCCT 
GTGCTGAGCG GAGACTTCTT CGCTTATGCA GACAGAGAGG ACCATTACTG GAGTGGATAC 
TACACCTCGC GACCCTTCTA CAAAAACCTG GACCGCATTT TAGAATCTCA CCTAAGAGGG 
GCAGAAATCC TCTATAGTTT GGCGGTGGCT CATGCACGAC ATGCAGGAGT TGAAGGACGA 
TATCCGGTTT CAGATTACTC TCTCCTGACT GATGCCAGGC GAAACATTGG GCTGTTCCAG 
CATCATGATG CCATCACGGG CACTGCCAAA GAGGCTGTCG TCATCGACTA CGGGAACAGG 
CTGTTACGTT CTCTGGTGGG TCTGAAACGA GTGATCATTA ATGCAGCGCA CTTCCTGATA 
ATAAAGAATA AAGACATTTA TCGCTTCTAC CAGACAGAGC CCTTCTTGGA GACGGATGAT 
AGACGCGCCA CACAGGACTC TCTCCCGCAG CGTACGCTTA TCGAGCTGGA ATCTTCACCA 
AGGTATCTGG TGCTGTTTAA CCCGGTGGAG CAGGAGCGTC TGTGTTTGGT CACTGTCCTG 
GTGAATTCGG CAAGAGTGAG GGTGCTTACA GAAGACGGAC AAACGCTACC GGTGCAGCTC 
AGCGCGCAGT GGATGTCCGC TACGGAGATG AGTGGGGAGG TTTATCAGGC TTCTTTCATG 
GCACGTCTGC CGGCTCTGGG ATTGGCCGTC TTCCACCTGT ACGACTCTGC TGATTCGCCC 
ATGACTCTGC GCTCCGAGAC GCTGCTCAGG GTTCCAGGAC GGGGTCAGAG CATTCGGGGT 
TTGGATCCTC TGCCTGTGCG CTCACAAACT GTTGATGGAC AGCCGTTCTA CATCCAAAGC 
CAATCCCTCA CTCTGGGCTT CTCTGGAGCC ACAGGGCTGC TGGAGAGCAT TCGACGGAAA 
GACGATCCCC AGGAGGTGAG GGTACAGATT CAGTTCCTCA CCTACGGTAC GCGACCCTCC 
AAGGACAAGA GCGGGGCCTA TCTGTTCCTG CCGGATGGAA ATGCTAAGCC GTATTCGCAG 
AGAGAGGCCC CTATCGTGCG TGTGGTTGAA GGTCCTCTGT TCGCAGAGGT TGTGGCACAT 
TATCAGCACT TCCAGCAGAC GGTCCGCATT CATAATGTTC CAGGGGTTGA CGGTCTTTCT 
TTGGACATCA CCACTATGGT GGACATCAGA GATCAGAATA ACAAAGAGCT GGCCATGAGG 
TTCGTCACCG ACATCCAGAG CGGAGATACT TTCTACACGG ATCTCAACGG CTTTCAGATT 
CAGCCTCGGC GATACTTCCA GAAACTTCCA CTACAAGCCA ATTTCTACCC CATGCCCAGC 
ACAGCATACA TCCAGGACAG CCAGTACCGG CTCACGCTCC ACACGGCACA GGCTCTGGGG 
GTTACCAGTC TGGCCAGCGG TCAGCTGGAG GTAATCCTGG ACCGCAGACT GATGCAGGAT 
GATAACAGGG GATTGGGACA AGGCCTGAAA GACAACAAAC GGACAGCTAA CAGGTTCCGT 
CTTCTGCTGG AGAGGCGGAG CAACAGTGCA AAGCCTGCTG GCTCCTATGC CAACATGCTA 
AACAAAATCA TTGCTGGTAT TTTAGGAGAC GGCCAGGAAG AGCCGGTGGA CAGCAGACCG 
GTCAGCTTCC CGTCTCTGCT CAGTCACATG ACCTCCACCA TCCTCAACCA TGAGGTGCTG 
GCACTGCCCG TGTTGCCCAG GAAACATGGC GTGCCACCCA TGCGAACCTT CGCCCCATTG 
GCTGGCACTC TGCCCTGCGA CTTCCACCTG CTGAACCTCA GGAGCATCCA AAGCCCGCAG 
GACGCTCACT CTCCATCTCC CTATATGGCT TTGCTGCTGC ACAGACTGGG TTTGGACTGT 
GGTCTGGAGC CGCAGAATCC TGGTTTCAAC TGCACCACCA CACAAGGCCA GCTCTCCGTT 
TCTGGGCTGT TCCGTGGGCT GGATCTCCAG CTCCTGCAGC CCATGTCTCT GTCTCTGATG 
CACTCTCAGC CTCCGCTCTC TAACGACTCC TCCGTCAGTC TGGAGCCAAT GGAGATCTCC 
GCCTACAAGC TCAAGCTGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_009297803.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296078.1 Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA.

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CloneID	ODa49902
Clone ID Related Accession (Same CDS sequence)	XM_009297803.1 , XM_009297803.2
Accession Version	XM_009297803.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alpha-mannosidase 2x isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC 
CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT 
ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG 
CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT 
CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT 
GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG 
TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG 
ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG 
ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC 
AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT 
GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT 
TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG 
ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG 
CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG 
CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG 
AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC 
GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC 
AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT 
GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG 
GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG 
AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG 
TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG 
GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG 
GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT 
TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA 
CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG 
CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC 
GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT 
AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG 
CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT 
ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT 
CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA 
CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG 
GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG 
TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA 
CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA 
CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG 
CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC 
ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA 
AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG 
TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT 
CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT 
AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG 
GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC 
AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC 
CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG 
GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA 
CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCGGTG 
GACAGCAGAC CGGTCAGCTT CCCGTCTCTG CTCAGTCACA TGACCTCCAC CATCCTCAAC 
CATGAGGTGC TGGCACTGCC CGTGTTGCCC AGGAAACATG GCGTGCCACC CATGCGAACC 
TTCGCCCCAT TGGCTGGCAC TCTGCCCTGC GACTTCCACC TGCTGAACCT CAGGAGCATC 
CAAAGCCCGC AGGACGCTCA CTCTCCATCT CCCTATATGG CTTTGCTGCT GCACAGACTG 
GGTTTGGACT GTGGTCTGGA GCCGCAGAAT CCTGGTTTCA ACTGCACCAC CACACAAGGC 
CAGCTCTCCG TTTCTGGGCT GTTCCGTGGG CTGGATCTCC AGCTCCTGCA GCCCATGTCT 
CTGTCTCTGA TGCACTCTCA GCCTCCGCTC TCTAACGACT CCTCCGTCAG TCTGGAGCCA 
ATGGAGATCT CCGCCTACAA GCTCAAGCTG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296078.1
CDS	788..4243
Translation	MKLKKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQNGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVLELTDTGAISPSGHLPFRSANGSWVLPFDGRPTFLSVKPLDCQFALGRRSQTDLQMLDVYSLLNFDNVDGGVWKQGFEITYEPNEWDDEPLQVFVVPHSHNDPGWIKTFDKYYNDQTQHILNNMVTKMAEDPRRKFIWSEISYFAKWWESADVHKQEVMRKLILSGQLEMVTGGWVMTDEANAHYFAMIDQLIEGHQWLERNLGVTPRSGWAVDPFGHSATMPYLLKRANISSMLIQRVHYSIKKHFAATRSLEFMWRQAWDIESSTDMFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGSRVNCPWKVPPRAINDANVAERAGVLLDQYRKKSKLFRSKVLLVPLGDDFRYDKALEWDQQYLNYQKLFDYMNSHPEMHVKAQFATLSDYFDAVYKANGVTSGARPPDYPVLSGDFFAYADREDHYWSGYYTSRPFYKNLDRILESHLRGAEILYSLAVAHARHAGVEGRYPVSDYSLLTDARRNIGLFQHHDAITGTAKEAVVIDYGNRLLRSLVGLKRVIINAAHFLIIKNKDIYRFYQTEPFLETDDRRATQDSLPQRTLIELESSPRYLVLFNPVEQERLCLVTVLVNSARVRVLTEDGQTLPVQLSAQWMSATEMSGEVYQASFMARLPALGLAVFHLYDSADSPMTLRSETLLRVPGRGQSIRGLDPLPVRSQTVDGQPFYIQSQSLTLGFSGATGLLESIRRKDDPQEVRVQIQFLTYGTRPSKDKSGAYLFLPDGNAKPYSQREAPIVRVVEGPLFAEVVAHYQHFQQTVRIHNVPGVDGLSLDITTMVDIRDQNNKELAMRFVTDIQSGDTFYTDLNGFQIQPRRYFQKLPLQANFYPMPSTAYIQDSQYRLTLHTAQALGVTSLASGQLEVILDRRLMQDDNRGLGQGLKDNKRTANRFRLLLERRSNSAKPVDSRPVSFPSLLSHMTSTILNHEVLALPVLPRKHGVPPMRTFAPLAGTLPCDFHLLNLRSIQSPQDAHSPSPYMALLLHRLGLDCGLEPQNPGFNCTTTQGQLSVSGLFRGLDLQLLQPMSLSLMHSQPPLSNDSSVSLEPMEISAYKLKLC

Target ORF information:

RefSeq Version	XM_009297803.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297803.1

ORF Insert Sequence:

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC 
CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT 
ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG 
CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT 
CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT 
GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG 
TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG 
ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG 
ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC 
AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT 
GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT 
TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG 
ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG 
CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG 
CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG 
AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC 
GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC 
AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT 
GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG 
GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG 
AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG 
TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG 
GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG 
GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT 
TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA 
CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG 
CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC 
GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT 
AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG 
CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT 
ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT 
CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA 
CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG 
GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG 
TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA 
CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA 
CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG 
CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC 
ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA 
AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG 
TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT 
CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT 
AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG 
GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC 
AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC 
CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG 
GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA 
CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCGGTG 
GACAGCAGAC CGGTCAGCTT CCCGTCTCTG CTCAGTCACA TGACCTCCAC CATCCTCAAC 
CATGAGGTGC TGGCACTGCC CGTGTTGCCC AGGAAACATG GCGTGCCACC CATGCGAACC 
TTCGCCCCAT TGGCTGGCAC TCTGCCCTGC GACTTCCACC TGCTGAACCT CAGGAGCATC 
CAAAGCCCGC AGGACGCTCA CTCTCCATCT CCCTATATGG CTTTGCTGCT GCACAGACTG 
GGTTTGGACT GTGGTCTGGA GCCGCAGAAT CCTGGTTTCA ACTGCACCAC CACACAAGGC 
CAGCTCTCCG TTTCTGGGCT GTTCCGTGGG CTGGATCTCC AGCTCCTGCA GCCCATGTCT 
CTGTCTCTGA TGCACTCTCA GCCTCCGCTC TCTAACGACT CCTCCGTCAG TCTGGAGCCA 
ATGGAGATCT CCGCCTACAA GCTCAAGCTG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_009297803.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296078.1 Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA.

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CloneID	ODa49902
Clone ID Related Accession (Same CDS sequence)	XM_009297803.1 , XM_009297803.2
Accession Version	XM_009297803.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-mannosidase 2x isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC 
CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT 
ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG 
CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT 
CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT 
GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG 
TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG 
ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG 
ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC 
AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT 
GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT 
TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG 
ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG 
CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG 
CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG 
AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC 
GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC 
AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT 
GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG 
GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG 
AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG 
TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG 
GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG 
GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT 
TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA 
CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG 
CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC 
GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT 
AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG 
CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT 
ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT 
CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA 
CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG 
GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG 
TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA 
CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA 
CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG 
CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC 
ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA 
AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG 
TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT 
CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT 
AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG 
GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC 
AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC 
CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG 
GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA 
CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCGGTG 
GACAGCAGAC CGGTCAGCTT CCCGTCTCTG CTCAGTCACA TGACCTCCAC CATCCTCAAC 
CATGAGGTGC TGGCACTGCC CGTGTTGCCC AGGAAACATG GCGTGCCACC CATGCGAACC 
TTCGCCCCAT TGGCTGGCAC TCTGCCCTGC GACTTCCACC TGCTGAACCT CAGGAGCATC 
CAAAGCCCGC AGGACGCTCA CTCTCCATCT CCCTATATGG CTTTGCTGCT GCACAGACTG 
GGTTTGGACT GTGGTCTGGA GCCGCAGAAT CCTGGTTTCA ACTGCACCAC CACACAAGGC 
CAGCTCTCCG TTTCTGGGCT GTTCCGTGGG CTGGATCTCC AGCTCCTGCA GCCCATGTCT 
CTGTCTCTGA TGCACTCTCA GCCTCCGCTC TCTAACGACT CCTCCGTCAG TCTGGAGCCA 
ATGGAGATCT CCGCCTACAA GCTCAAGCTG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296078.1
CDS	788..4243
Translation	MKLKKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQNGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVLELTDTGAISPSGHLPFRSANGSWVLPFDGRPTFLSVKPLDCQFALGRRSQTDLQMLDVYSLLNFDNVDGGVWKQGFEITYEPNEWDDEPLQVFVVPHSHNDPGWIKTFDKYYNDQTQHILNNMVTKMAEDPRRKFIWSEISYFAKWWESADVHKQEVMRKLILSGQLEMVTGGWVMTDEANAHYFAMIDQLIEGHQWLERNLGVTPRSGWAVDPFGHSATMPYLLKRANISSMLIQRVHYSIKKHFAATRSLEFMWRQAWDIESSTDMFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGSRVNCPWKVPPRAINDANVAERAGVLLDQYRKKSKLFRSKVLLVPLGDDFRYDKALEWDQQYLNYQKLFDYMNSHPEMHVKAQFATLSDYFDAVYKANGVTSGARPPDYPVLSGDFFAYADREDHYWSGYYTSRPFYKNLDRILESHLRGAEILYSLAVAHARHAGVEGRYPVSDYSLLTDARRNIGLFQHHDAITGTAKEAVVIDYGNRLLRSLVGLKRVIINAAHFLIIKNKDIYRFYQTEPFLETDDRRATQDSLPQRTLIELESSPRYLVLFNPVEQERLCLVTVLVNSARVRVLTEDGQTLPVQLSAQWMSATEMSGEVYQASFMARLPALGLAVFHLYDSADSPMTLRSETLLRVPGRGQSIRGLDPLPVRSQTVDGQPFYIQSQSLTLGFSGATGLLESIRRKDDPQEVRVQIQFLTYGTRPSKDKSGAYLFLPDGNAKPYSQREAPIVRVVEGPLFAEVVAHYQHFQQTVRIHNVPGVDGLSLDITTMVDIRDQNNKELAMRFVTDIQSGDTFYTDLNGFQIQPRRYFQKLPLQANFYPMPSTAYIQDSQYRLTLHTAQALGVTSLASGQLEVILDRRLMQDDNRGLGQGLKDNKRTANRFRLLLERRSNSAKPVDSRPVSFPSLLSHMTSTILNHEVLALPVLPRKHGVPPMRTFAPLAGTLPCDFHLLNLRSIQSPQDAHSPSPYMALLLHRLGLDCGLEPQNPGFNCTTTQGQLSVSGLFRGLDLQLLQPMSLSLMHSQPPLSNDSSVSLEPMEISAYKLKLC

Target ORF information:

RefSeq Version	XM_009297803.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297803.2

ORF Insert Sequence:

ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG 
AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC 
CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT 
ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG 
CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT 
CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT 
GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG 
TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG 
ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG 
ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC 
AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT 
GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT 
TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG 
ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG 
CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG 
CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG 
AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC 
GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC 
AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT 
GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG 
GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG 
AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG 
TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG 
GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG 
GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT 
TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA 
CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG 
CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC 
GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT 
AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG 
CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT 
ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT 
CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA 
CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG 
GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG 
TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA 
CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA 
CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG 
CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC 
ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA 
AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG 
TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT 
CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT 
AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG 
GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC 
AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC 
CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG 
GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA 
CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCGGTG 
GACAGCAGAC CGGTCAGCTT CCCGTCTCTG CTCAGTCACA TGACCTCCAC CATCCTCAAC 
CATGAGGTGC TGGCACTGCC CGTGTTGCCC AGGAAACATG GCGTGCCACC CATGCGAACC 
TTCGCCCCAT TGGCTGGCAC TCTGCCCTGC GACTTCCACC TGCTGAACCT CAGGAGCATC 
CAAAGCCCGC AGGACGCTCA CTCTCCATCT CCCTATATGG CTTTGCTGCT GCACAGACTG 
GGTTTGGACT GTGGTCTGGA GCCGCAGAAT CCTGGTTTCA ACTGCACCAC CACACAAGGC 
CAGCTCTCCG TTTCTGGGCT GTTCCGTGGG CTGGATCTCC AGCTCCTGCA GCCCATGTCT 
CTGTCTCTGA TGCACTCTCA GCCTCCGCTC TCTAACGACT CCTCCGTCAG TCTGGAGCCA 
ATGGAGATCT CCGCCTACAA GCTCAAGCTG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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man2a2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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