HLCS cDNA ORF clone, Canis lupus familiaris(dog)

The following HLCS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLCS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe29421 XM_022413367.1
Latest version!
Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe29421 XM_014109780.1
Latest version!
Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe29421 XM_022413368.1
Latest version!
Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe29421 XM_005638902.2
Latest version!
Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe29421 XM_014109781.2
Latest version!
Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe29421 XM_014109784.2
Latest version!
Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe29421 XM_022413369.1
Latest version!
Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe29421 XM_014109783.1
Latest version!
Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe29421 XM_014109782.1
Latest version!
Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe29421 XM_535590.5
Latest version!
Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe29421 XM_022413370.1
Latest version!
Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OCe29421 XM_014109784.1 Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe29421 XM_014109781.1 Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_022413367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product biotin--protein ligase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269075.1
    CDS719..2893
    Translation

    Target ORF information:

    RefSeq Version XM_022413367.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_014109784.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product biotin--protein ligase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109784.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965259.1
    CDS374..2548
    Translation

    Target ORF information:

    RefSeq Version XM_014109784.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109784.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_535590.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product biotin--protein ligase
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_535590.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_535590.3
    CDS1209..3383
    Translation

    Target ORF information:

    RefSeq Version XM_535590.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_535590.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_014109782.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product biotin--protein ligase
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965257.1
    CDS480..2654
    Translation

    Target ORF information:

    RefSeq Version XM_014109782.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_014109784.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product biotin--protein ligase
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965259.1
    CDS291..2465
    Translation

    Target ORF information:

    RefSeq Version XM_014109784.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_022413368.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product biotin--protein ligase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269076.1
    CDS574..2748
    Translation

    Target ORF information:

    RefSeq Version XM_022413368.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_022413370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product biotin--protein ligase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269078.1
    CDS403..2577
    Translation

    Target ORF information:

    RefSeq Version XM_022413370.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_022413369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product biotin--protein ligase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269077.1
    CDS654..2828
    Translation

    Target ORF information:

    RefSeq Version XM_022413369.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_014109780.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product biotin--protein ligase
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965255.1
    CDS618..2792
    Translation

    Target ORF information:

    RefSeq Version XM_014109780.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109780.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_014109781.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product biotin--protein ligase
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965256.1
    CDS704..2878
    Translation

    Target ORF information:

    RefSeq Version XM_014109781.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_014109783.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product biotin--protein ligase
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965258.1
    CDS352..2526
    Translation

    Target ORF information:

    RefSeq Version XM_014109783.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_005638902.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product biotin--protein ligase
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638959.1
    CDS580..2754
    Translation

    Target ORF information:

    RefSeq Version XM_005638902.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638902.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29421
    Clone ID Related Accession (Same CDS sequence) XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
    Accession Version XM_014109781.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product biotin--protein ligase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965256.1
    CDS691..2865
    Translation

    Target ORF information:

    RefSeq Version XM_014109781.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109781.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
    TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG
    CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT
    AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC
    AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT
    CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT
    GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT
    GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC
    ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG
    TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA
    AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG
    GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG
    TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC
    AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC
    ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG
    CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG
    ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA
    GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA
    GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC
    TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG
    CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT
    CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC
    GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA
    AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG
    GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA
    ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC
    ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG
    CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC
    AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA
    GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT
    AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG
    CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT
    GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT
    TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA
    GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC
    GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC
    CCCAAGCAGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.