Hlcs cDNA ORF clone, Mus musculus(house mouse)

The following Hlcs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hlcs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu10948 NM_139145.5
Latest version!
Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OMu10948 XM_006522855.2
Latest version!
Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OMu10948 XM_006522856.2
Latest version!
Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OMu84228 NM_001360045.1
Latest version!
Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu10948 XM_030248919.1
Latest version!
Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu10948 XM_030248920.1
Latest version!
Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu52627 XM_006522851.4
Latest version!
Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu52628 XM_006522852.4
Latest version!
Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu52629 XM_006522853.4
Latest version!
Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu52630 XM_006522854.4
Latest version!
Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu86856 XM_030248921.1
Latest version!
Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu52626 XM_006522850.3
Latest version!
Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
View Old Accession Versions >>
OMu10948 XM_006522856.1 Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OMu52627 XM_006522851.3 Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu52628 XM_006522852.3 Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu52629 XM_006522853.3 Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OMu10948 XM_006522855.1 Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
OMu52630 XM_006522854.3 Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OMu10948 NM_139145.4 Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu10948
    Clone ID Related Accession (Same CDS sequence) XM_006522855.1 , XM_006522856.2 , XM_006522856.1 , NM_139145.4 , XM_030248919.1 , XM_006522855.2 , XM_030248920.1 , NM_139145.5
    Accession Version NM_139145.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CT573086.9 and CU207273.8. On May 1, 2007 this sequence version replaced NM_139145.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC050090.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_631884.1
    CDS177..2345
    Translation

    Target ORF information:

    RefSeq Version NM_139145.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_139145.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu10948
    Clone ID Related Accession (Same CDS sequence) XM_006522855.1 , XM_006522856.2 , XM_006522856.1 , NM_139145.4 , XM_030248919.1 , XM_006522855.2 , XM_030248920.1 , NM_139145.5
    Accession Version NM_139145.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT573086.9 and CU207273.8. On Jan 26, 2018 this sequence version replaced NM_139145.4. Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK158212.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_631884.1
    CDS293..2461
    Translation

    Target ORF information:

    RefSeq Version NM_139145.5
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_139145.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu10948
    Clone ID Related Accession (Same CDS sequence) XM_006522855.1 , XM_006522856.2 , XM_006522856.1 , NM_139145.4 , XM_030248919.1 , XM_006522855.2 , XM_030248920.1 , NM_139145.5
    Accession Version XM_006522855.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product biotin--protein ligase isoform X6
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522918.1
    Translation

    Target ORF information:

    RefSeq Version XM_006522855.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522855.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu10948
    Clone ID Related Accession (Same CDS sequence) XM_006522855.1 , XM_006522856.2 , XM_006522856.1 , NM_139145.4 , XM_030248919.1 , XM_006522855.2 , XM_030248920.1 , NM_139145.5
    Accession Version XM_006522856.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522919.1
    CDS720..2888
    Translation

    Target ORF information:

    RefSeq Version XM_006522856.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522856.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu10948
    Clone ID Related Accession (Same CDS sequence) XM_006522855.1 , XM_006522856.2 , XM_006522856.1 , NM_139145.4 , XM_030248919.1 , XM_006522855.2 , XM_030248920.1 , NM_139145.5
    Accession Version XM_006522856.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product biotin--protein ligase isoform X6
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522919.1
    Translation

    Target ORF information:

    RefSeq Version XM_006522856.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522856.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu10948
    Clone ID Related Accession (Same CDS sequence) XM_006522855.1 , XM_006522856.2 , XM_006522856.1 , NM_139145.4 , XM_030248919.1 , XM_006522855.2 , XM_030248920.1 , NM_139145.5
    Accession Version XM_006522855.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522918.1
    CDS820..2988
    Translation

    Target ORF information:

    RefSeq Version XM_006522855.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522855.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52627
    Clone ID Related Accession (Same CDS sequence) XM_006522851.3 , XM_006522851.4
    Accession Version XM_006522851.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522851.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTGTCTGG CACGCGGTGC TGCGCGAATG GCAAGCCTGG CTTTGCAGGG GACAGGGAGC 
    TGGGGATCCA GATTGCAGGG GCAGATGGCA GACTGGCTGC GCGTCCCCGA CGCAAGGGCA
    GCTTTGCGCA GTGCGCATCC CCGCGCGCTG CTCGCCGCGG CCGCCAGGGG GCGCAGCGGG
    ATCTCAGTGC GATCACCCTG CGGAGCGGGC AGCCGCGCTC AGGGCTGCGA GTCTGTTGAT
    GACTTGAACA AATGGGCCCT GTTTCTCGTG TCTCCCTTCA TACTTGAGGC AGAACATATA
    GCATTTGTGA CAGAGAGCAT TTGGATACAA GGTACCAGTT TACAAGAGCC ATCATCTTCA
    GAAACCGTTG TAAAATGGTC TGACTGCTGT CTGCCGTTAG CCTGCCGACC CGGGGACCCC
    TACCAGTTAA TTGCTAAGGC AAGCGTGGAC AACTTCAGCA AGCTTGGGGT GGCCTTCATG
    GAGGACAGGC TCCAGATGGA CAACGGCCTG ATAGCCCAGA AGATCGTTTC AGTCCACTTA
    AAGGACCCTG CTCTGAAGGA ACTCGGCAAA GCCTCCGACA AGCAAGTGCA GGGCCCGCCC
    CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG CCTGCACAAG GTGTCATGGA GCATGCTGGC
    CAAGGTGATT GCAAGGCCGC CGGTGAAGGC CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT
    GAAAGTGAGC CTGCTGCAGA TGGGGACCCA GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC
    CACCTGTCCA TCTGTCACGA GTGTCTGGAG CTCGAGAACA GTACCATTGA CTCGGTGAGG
    TCTGCGTCTG CGGAGAACAT TCCAGACCTT CCCTGTGATC ACAGCGGCGT GGAGGGTGCT
    GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG AAGAGAGTCA ACATTTCGGG AAAGGCGCCC
    AATATCTTGC TGTATGTGGG CTCTGGTTCC GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG
    CGATCTGTCC TGACTGACTG TGTGGACACG GACAGCTACA CTCTCTACCA CCTGCTGGAA
    GACAGCGCTC TGAGGGACCC GTGGTCTGAC AACTGCCTCC TGCTAGTTAT TGCCAGCAGG
    GACCCCATCC CCAAAGACAT ACAGCACAAG TTCATGGCCT ATCTTTCTCA GGGAGGGAAG
    GTGCTGGGCC TGTCTTCCCC CTTCACGTTG GGTGGCTTTC GGGTGACCAG GAGAGATGTG
    CTACGGAACA CAGTCCAGAA CTTGGTTTTC TCTAAGGCTG ATGGGACTGA GGTACGGCTC
    AGTGTCCTGA GCAGCGGCTA CGTTTATGAA GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC
    CACCTGGAGA ATGAGGACAA GGATAAAATG ATTGTGCACG TGCCTTTTGG AACCCTCGGA
    GGGGAAGCCG TTCTCTGCCA GGTGCACCTG GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG
    ACTGCAGACG ACTTTAACGT GCTCAAATCC AGCAATGTGA GAAGACACGA GGTCCTTAAG
    GAGATCCTGA CTGCCCTCGG TCTGAGCTGT GACGCACCAC AGGTTCCTGC CTTAACGCCC
    CTGTACTTAC TGCTGGCCGC TGAGGAAACC CAGGATCCGT TCATGCAGTG GCTCGGGAGG
    CACACAGATC CTGAAGGAAT CATAAAATCC AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC
    TACACATCTG AAGCGGAGAT CACCCCGTCT TCCATGCCTG TGGTCACTGA CCCAGAAGCC
    TTTTCATCAG AGCACTTCAG CCTAGAGACC TATCGACAGA ATCTGCAGAC CACGCGCCTT
    GGGAAAGTGA TTCTGTTTGC AGAAGTGACC TCCACCACCA TGAGTCTCCT GGATGGGTTG
    ATGTTTGAGA TGCCGCAGGA AATGGGCTTA ATTGCCATTG CAGTTCGGCA GACCCAGGGC
    AAAGGAAGGG GCCCGAATGC CTGGCTGAGC CCCGTGGGAT GTGCTCTTTC CACTCTGCTG
    GTCTTCATCC CCCTGAGGTC ACAACTGGGA CAGAGGATTC CATTTGTCCA GCATTTGATG
    TCCTTGGCTG TGGTGGAGGC AGTGCGGTCT ATCCCAGGGT ATGAGGATAT CAACTTGCGA
    GTGAAGTGGC CCAACGATAT TTATTACAGC GATCTCATGA AGATCGGTGG GGTTCTGGTT
    AATTCAACAC TTATGGGAGA AACATTTTAT ATCCTCATCG GCTGTGGATT CAATGTGACG
    AACAGTAATC CTACCATTTG TATCAACGAC CTCATCGAAG AACACAATAA GCAGCACGGG
    GCGGGCCTGA AGCCCCTGCG AGCGGACTGT CTCATAGCCA GAGCCGTGAC CGTGCTGGAG
    AAACTGATCG ACAGGTTTCA GGACCAAGGG CCCGACGGCG TTCTTCCCCT CTATTATAAA
    TACTGGGTGC ACGGTGGTCA GCAAGTCCGC CTGGGCAGCA CGGAGGGGCC ACAGGCATCT
    ATTGTGGGCC TTGATGACTC CGGGTTCCTG CAGGTCCACC AAGAGGATGG TGGGGTTGTG
    ACGGTGCATC CAGATGGCAA CTCCTTCGAC ATGCTGAGAA ACCTCATCGT GCCCAAGCGA
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522914.1
    CDS166..2811
    Translation

    Target ORF information:

    RefSeq Version XM_006522851.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522851.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTGTCTGG CACGCGGTGC TGCGCGAATG GCAAGCCTGG CTTTGCAGGG GACAGGGAGC 
    TGGGGATCCA GATTGCAGGG GCAGATGGCA GACTGGCTGC GCGTCCCCGA CGCAAGGGCA
    GCTTTGCGCA GTGCGCATCC CCGCGCGCTG CTCGCCGCGG CCGCCAGGGG GCGCAGCGGG
    ATCTCAGTGC GATCACCCTG CGGAGCGGGC AGCCGCGCTC AGGGCTGCGA GTCTGTTGAT
    GACTTGAACA AATGGGCCCT GTTTCTCGTG TCTCCCTTCA TACTTGAGGC AGAACATATA
    GCATTTGTGA CAGAGAGCAT TTGGATACAA GGTACCAGTT TACAAGAGCC ATCATCTTCA
    GAAACCGTTG TAAAATGGTC TGACTGCTGT CTGCCGTTAG CCTGCCGACC CGGGGACCCC
    TACCAGTTAA TTGCTAAGGC AAGCGTGGAC AACTTCAGCA AGCTTGGGGT GGCCTTCATG
    GAGGACAGGC TCCAGATGGA CAACGGCCTG ATAGCCCAGA AGATCGTTTC AGTCCACTTA
    AAGGACCCTG CTCTGAAGGA ACTCGGCAAA GCCTCCGACA AGCAAGTGCA GGGCCCGCCC
    CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG CCTGCACAAG GTGTCATGGA GCATGCTGGC
    CAAGGTGATT GCAAGGCCGC CGGTGAAGGC CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT
    GAAAGTGAGC CTGCTGCAGA TGGGGACCCA GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC
    CACCTGTCCA TCTGTCACGA GTGTCTGGAG CTCGAGAACA GTACCATTGA CTCGGTGAGG
    TCTGCGTCTG CGGAGAACAT TCCAGACCTT CCCTGTGATC ACAGCGGCGT GGAGGGTGCT
    GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG AAGAGAGTCA ACATTTCGGG AAAGGCGCCC
    AATATCTTGC TGTATGTGGG CTCTGGTTCC GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG
    CGATCTGTCC TGACTGACTG TGTGGACACG GACAGCTACA CTCTCTACCA CCTGCTGGAA
    GACAGCGCTC TGAGGGACCC GTGGTCTGAC AACTGCCTCC TGCTAGTTAT TGCCAGCAGG
    GACCCCATCC CCAAAGACAT ACAGCACAAG TTCATGGCCT ATCTTTCTCA GGGAGGGAAG
    GTGCTGGGCC TGTCTTCCCC CTTCACGTTG GGTGGCTTTC GGGTGACCAG GAGAGATGTG
    CTACGGAACA CAGTCCAGAA CTTGGTTTTC TCTAAGGCTG ATGGGACTGA GGTACGGCTC
    AGTGTCCTGA GCAGCGGCTA CGTTTATGAA GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC
    CACCTGGAGA ATGAGGACAA GGATAAAATG ATTGTGCACG TGCCTTTTGG AACCCTCGGA
    GGGGAAGCCG TTCTCTGCCA GGTGCACCTG GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG
    ACTGCAGACG ACTTTAACGT GCTCAAATCC AGCAATGTGA GAAGACACGA GGTCCTTAAG
    GAGATCCTGA CTGCCCTCGG TCTGAGCTGT GACGCACCAC AGGTTCCTGC CTTAACGCCC
    CTGTACTTAC TGCTGGCCGC TGAGGAAACC CAGGATCCGT TCATGCAGTG GCTCGGGAGG
    CACACAGATC CTGAAGGAAT CATAAAATCC AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC
    TACACATCTG AAGCGGAGAT CACCCCGTCT TCCATGCCTG TGGTCACTGA CCCAGAAGCC
    TTTTCATCAG AGCACTTCAG CCTAGAGACC TATCGACAGA ATCTGCAGAC CACGCGCCTT
    GGGAAAGTGA TTCTGTTTGC AGAAGTGACC TCCACCACCA TGAGTCTCCT GGATGGGTTG
    ATGTTTGAGA TGCCGCAGGA AATGGGCTTA ATTGCCATTG CAGTTCGGCA GACCCAGGGC
    AAAGGAAGGG GCCCGAATGC CTGGCTGAGC CCCGTGGGAT GTGCTCTTTC CACTCTGCTG
    GTCTTCATCC CCCTGAGGTC ACAACTGGGA CAGAGGATTC CATTTGTCCA GCATTTGATG
    TCCTTGGCTG TGGTGGAGGC AGTGCGGTCT ATCCCAGGGT ATGAGGATAT CAACTTGCGA
    GTGAAGTGGC CCAACGATAT TTATTACAGC GATCTCATGA AGATCGGTGG GGTTCTGGTT
    AATTCAACAC TTATGGGAGA AACATTTTAT ATCCTCATCG GCTGTGGATT CAATGTGACG
    AACAGTAATC CTACCATTTG TATCAACGAC CTCATCGAAG AACACAATAA GCAGCACGGG
    GCGGGCCTGA AGCCCCTGCG AGCGGACTGT CTCATAGCCA GAGCCGTGAC CGTGCTGGAG
    AAACTGATCG ACAGGTTTCA GGACCAAGGG CCCGACGGCG TTCTTCCCCT CTATTATAAA
    TACTGGGTGC ACGGTGGTCA GCAAGTCCGC CTGGGCAGCA CGGAGGGGCC ACAGGCATCT
    ATTGTGGGCC TTGATGACTC CGGGTTCCTG CAGGTCCACC AAGAGGATGG TGGGGTTGTG
    ACGGTGCATC CAGATGGCAA CTCCTTCGAC ATGCTGAGAA ACCTCATCGT GCCCAAGCGA
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52628
    Clone ID Related Accession (Same CDS sequence) XM_006522852.3 , XM_006522852.4
    Accession Version XM_006522852.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522852.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTGTCTGG CACGCGGTGC TGCGCGAATG GCAAGCCTGG CTTTGCAGGG GACAGGGAGC 
    TGGGGATCCA GATTGCAGGG GCAGATGGCA GACTGGCTGC GCGTCCCCGA CGCAAGGGCA
    GCTTTGCGCA GTGCGCATCC CCGCGCGCTG CTCGCCGCGG CCGCCAGGGG GCGCAGCGGG
    ATCTCAGTGC GATCACCCTG CGGAGCGGGC AGCCGCGCTC AGGGCTGCGA GGTTGTAAAA
    TGGTCTGACT GCTGTCTGCC GTTAGCCTGC CGACCCGGGG ACCCCTACCA GTTAATTGCT
    AAGGCAAGCG TGGACAACTT CAGCAAGCTT GGGGTGGCCT TCATGGAGGA CAGGCTCCAG
    ATGGACAACG GCCTGATAGC CCAGAAGATC GTTTCAGTCC ACTTAAAGGA CCCTGCTCTG
    AAGGAACTCG GCAAAGCCTC CGACAAGCAA GTGCAGGGCC CGCCCCCGGG CCCAGAAGCT
    TCCCCCGAGG CCCAGCCTGC ACAAGGTGTC ATGGAGCATG CTGGCCAAGG TGATTGCAAG
    GCCGCCGGTG AAGGCCCTTC CCCACGGAGG AGGGGCTGTG CCCCTGAAAG TGAGCCTGCT
    GCAGATGGGG ACCCAGGGCT GAGCTCCCCG GAGCTCTGTC AGCTCCACCT GTCCATCTGT
    CACGAGTGTC TGGAGCTCGA GAACAGTACC ATTGACTCGG TGAGGTCTGC GTCTGCGGAG
    AACATTCCAG ACCTTCCCTG TGATCACAGC GGCGTGGAGG GTGCTGCGGG CGAACTCTGC
    CCCGAACGGA AAGGGAAGAG AGTCAACATT TCGGGAAAGG CGCCCAATAT CTTGCTGTAT
    GTGGGCTCTG GTTCCGAGGA AGCCCTGGGC CGGCTCCAGC AGGTGCGATC TGTCCTGACT
    GACTGTGTGG ACACGGACAG CTACACTCTC TACCACCTGC TGGAAGACAG CGCTCTGAGG
    GACCCGTGGT CTGACAACTG CCTCCTGCTA GTTATTGCCA GCAGGGACCC CATCCCCAAA
    GACATACAGC ACAAGTTCAT GGCCTATCTT TCTCAGGGAG GGAAGGTGCT GGGCCTGTCT
    TCCCCCTTCA CGTTGGGTGG CTTTCGGGTG ACCAGGAGAG ATGTGCTACG GAACACAGTC
    CAGAACTTGG TTTTCTCTAA GGCTGATGGG ACTGAGGTAC GGCTCAGTGT CCTGAGCAGC
    GGCTACGTTT ATGAAGAAGG CCCGAGCTTG GGCAGGCTCC AGGGCCACCT GGAGAATGAG
    GACAAGGATA AAATGATTGT GCACGTGCCT TTTGGAACCC TCGGAGGGGA AGCCGTTCTC
    TGCCAGGTGC ACCTGGAGCT CCCGCCCGGC GCCTCCCTGG TGCAGACTGC AGACGACTTT
    AACGTGCTCA AATCCAGCAA TGTGAGAAGA CACGAGGTCC TTAAGGAGAT CCTGACTGCC
    CTCGGTCTGA GCTGTGACGC ACCACAGGTT CCTGCCTTAA CGCCCCTGTA CTTACTGCTG
    GCCGCTGAGG AAACCCAGGA TCCGTTCATG CAGTGGCTCG GGAGGCACAC AGATCCTGAA
    GGAATCATAA AATCCAGCAA GCTCTCCCTT CAGTTTGTTT CCTCCTACAC ATCTGAAGCG
    GAGATCACCC CGTCTTCCAT GCCTGTGGTC ACTGACCCAG AAGCCTTTTC ATCAGAGCAC
    TTCAGCCTAG AGACCTATCG ACAGAATCTG CAGACCACGC GCCTTGGGAA AGTGATTCTG
    TTTGCAGAAG TGACCTCCAC CACCATGAGT CTCCTGGATG GGTTGATGTT TGAGATGCCG
    CAGGAAATGG GCTTAATTGC CATTGCAGTT CGGCAGACCC AGGGCAAAGG AAGGGGCCCG
    AATGCCTGGC TGAGCCCCGT GGGATGTGCT CTTTCCACTC TGCTGGTCTT CATCCCCCTG
    AGGTCACAAC TGGGACAGAG GATTCCATTT GTCCAGCATT TGATGTCCTT GGCTGTGGTG
    GAGGCAGTGC GGTCTATCCC AGGGTATGAG GATATCAACT TGCGAGTGAA GTGGCCCAAC
    GATATTTATT ACAGCGATCT CATGAAGATC GGTGGGGTTC TGGTTAATTC AACACTTATG
    GGAGAAACAT TTTATATCCT CATCGGCTGT GGATTCAATG TGACGAACAG TAATCCTACC
    ATTTGTATCA ACGACCTCAT CGAAGAACAC AATAAGCAGC ACGGGGCGGG CCTGAAGCCC
    CTGCGAGCGG ACTGTCTCAT AGCCAGAGCC GTGACCGTGC TGGAGAAACT GATCGACAGG
    TTTCAGGACC AAGGGCCCGA CGGCGTTCTT CCCCTCTATT ATAAATACTG GGTGCACGGT
    GGTCAGCAAG TCCGCCTGGG CAGCACGGAG GGGCCACAGG CATCTATTGT GGGCCTTGAT
    GACTCCGGGT TCCTGCAGGT CCACCAAGAG GATGGTGGGG TTGTGACGGT GCATCCAGAT
    GGCAACTCCT TCGACATGCT GAGAAACCTC ATCGTGCCCA AGCGACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522915.1
    CDS166..2676
    Translation

    Target ORF information:

    RefSeq Version XM_006522852.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522852.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTGTCTGG CACGCGGTGC TGCGCGAATG GCAAGCCTGG CTTTGCAGGG GACAGGGAGC 
    TGGGGATCCA GATTGCAGGG GCAGATGGCA GACTGGCTGC GCGTCCCCGA CGCAAGGGCA
    GCTTTGCGCA GTGCGCATCC CCGCGCGCTG CTCGCCGCGG CCGCCAGGGG GCGCAGCGGG
    ATCTCAGTGC GATCACCCTG CGGAGCGGGC AGCCGCGCTC AGGGCTGCGA GGTTGTAAAA
    TGGTCTGACT GCTGTCTGCC GTTAGCCTGC CGACCCGGGG ACCCCTACCA GTTAATTGCT
    AAGGCAAGCG TGGACAACTT CAGCAAGCTT GGGGTGGCCT TCATGGAGGA CAGGCTCCAG
    ATGGACAACG GCCTGATAGC CCAGAAGATC GTTTCAGTCC ACTTAAAGGA CCCTGCTCTG
    AAGGAACTCG GCAAAGCCTC CGACAAGCAA GTGCAGGGCC CGCCCCCGGG CCCAGAAGCT
    TCCCCCGAGG CCCAGCCTGC ACAAGGTGTC ATGGAGCATG CTGGCCAAGG TGATTGCAAG
    GCCGCCGGTG AAGGCCCTTC CCCACGGAGG AGGGGCTGTG CCCCTGAAAG TGAGCCTGCT
    GCAGATGGGG ACCCAGGGCT GAGCTCCCCG GAGCTCTGTC AGCTCCACCT GTCCATCTGT
    CACGAGTGTC TGGAGCTCGA GAACAGTACC ATTGACTCGG TGAGGTCTGC GTCTGCGGAG
    AACATTCCAG ACCTTCCCTG TGATCACAGC GGCGTGGAGG GTGCTGCGGG CGAACTCTGC
    CCCGAACGGA AAGGGAAGAG AGTCAACATT TCGGGAAAGG CGCCCAATAT CTTGCTGTAT
    GTGGGCTCTG GTTCCGAGGA AGCCCTGGGC CGGCTCCAGC AGGTGCGATC TGTCCTGACT
    GACTGTGTGG ACACGGACAG CTACACTCTC TACCACCTGC TGGAAGACAG CGCTCTGAGG
    GACCCGTGGT CTGACAACTG CCTCCTGCTA GTTATTGCCA GCAGGGACCC CATCCCCAAA
    GACATACAGC ACAAGTTCAT GGCCTATCTT TCTCAGGGAG GGAAGGTGCT GGGCCTGTCT
    TCCCCCTTCA CGTTGGGTGG CTTTCGGGTG ACCAGGAGAG ATGTGCTACG GAACACAGTC
    CAGAACTTGG TTTTCTCTAA GGCTGATGGG ACTGAGGTAC GGCTCAGTGT CCTGAGCAGC
    GGCTACGTTT ATGAAGAAGG CCCGAGCTTG GGCAGGCTCC AGGGCCACCT GGAGAATGAG
    GACAAGGATA AAATGATTGT GCACGTGCCT TTTGGAACCC TCGGAGGGGA AGCCGTTCTC
    TGCCAGGTGC ACCTGGAGCT CCCGCCCGGC GCCTCCCTGG TGCAGACTGC AGACGACTTT
    AACGTGCTCA AATCCAGCAA TGTGAGAAGA CACGAGGTCC TTAAGGAGAT CCTGACTGCC
    CTCGGTCTGA GCTGTGACGC ACCACAGGTT CCTGCCTTAA CGCCCCTGTA CTTACTGCTG
    GCCGCTGAGG AAACCCAGGA TCCGTTCATG CAGTGGCTCG GGAGGCACAC AGATCCTGAA
    GGAATCATAA AATCCAGCAA GCTCTCCCTT CAGTTTGTTT CCTCCTACAC ATCTGAAGCG
    GAGATCACCC CGTCTTCCAT GCCTGTGGTC ACTGACCCAG AAGCCTTTTC ATCAGAGCAC
    TTCAGCCTAG AGACCTATCG ACAGAATCTG CAGACCACGC GCCTTGGGAA AGTGATTCTG
    TTTGCAGAAG TGACCTCCAC CACCATGAGT CTCCTGGATG GGTTGATGTT TGAGATGCCG
    CAGGAAATGG GCTTAATTGC CATTGCAGTT CGGCAGACCC AGGGCAAAGG AAGGGGCCCG
    AATGCCTGGC TGAGCCCCGT GGGATGTGCT CTTTCCACTC TGCTGGTCTT CATCCCCCTG
    AGGTCACAAC TGGGACAGAG GATTCCATTT GTCCAGCATT TGATGTCCTT GGCTGTGGTG
    GAGGCAGTGC GGTCTATCCC AGGGTATGAG GATATCAACT TGCGAGTGAA GTGGCCCAAC
    GATATTTATT ACAGCGATCT CATGAAGATC GGTGGGGTTC TGGTTAATTC AACACTTATG
    GGAGAAACAT TTTATATCCT CATCGGCTGT GGATTCAATG TGACGAACAG TAATCCTACC
    ATTTGTATCA ACGACCTCAT CGAAGAACAC AATAAGCAGC ACGGGGCGGG CCTGAAGCCC
    CTGCGAGCGG ACTGTCTCAT AGCCAGAGCC GTGACCGTGC TGGAGAAACT GATCGACAGG
    TTTCAGGACC AAGGGCCCGA CGGCGTTCTT CCCCTCTATT ATAAATACTG GGTGCACGGT
    GGTCAGCAAG TCCGCCTGGG CAGCACGGAG GGGCCACAGG CATCTATTGT GGGCCTTGAT
    GACTCCGGGT TCCTGCAGGT CCACCAAGAG GATGGTGGGG TTGTGACGGT GCATCCAGAT
    GGCAACTCCT TCGACATGCT GAGAAACCTC ATCGTGCCCA AGCGACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu84228
    Clone ID Related Accession (Same CDS sequence) NM_001360045.1
    Accession Version NM_001360045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-24
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT573086.9 and CU207273.8. On Jan 26, 2018 this sequence version replaced XM_006522850.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK052424.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCCG GCCGGCCGCG 
    CTGGTGCTGT CCACGGTGCG GCGGCTGCGT GCTTCGCGCT GCTCCTTCAC CTTCTGCCGC
    GCGGCCGCGC AGCCCAGGGA CGCGCGCGTG TGCCTGAGCC AGGGCGGCCG CGTCTTCTGC
    GTCGGCGAGA GCCAGTCTGT TGATGACTTG AACAAATGGG CCCTGTTTCT CGTGTCTCCC
    TTCATACTTG AGGCAGAACA TATAGCATTT GTGACAGAGA GCATTTGGAT ACAAGGTACC
    AGTTTACAAG AGCCATCATC TTCAGAAACC GTTGTAAAAT GGTCTGACTG CTGTCTGCCG
    TTAGCCTGCC GACCCGGGGA CCCCTACCAG TTAATTGCTA AGGCAAGCGT GGACAACTTC
    AGCAAGCTTG GGGTGGCCTT CATGGAGGAC AGGCTCCAGA TGGACAACGG CCTGATAGCC
    CAGAAGATCG TTTCAGTCCA CTTAAAGGAC CCTGCTCTGA AGGAACTCGG CAAAGCCTCC
    GACAAGCAAG TGCAGGGCCC GCCCCCGGGC CCAGAAGCTT CCCCCGAGGC CCAGCCTGCA
    CAAGGTGTCA TGGAGCATGC TGGCCAAGGT GATTGCAAGG CCGCCGGTGA AGGCCCTTCC
    CCACGGAGGA GGGGCTGTGC CCCTGAAAGT GAGCCTGCTG CAGATGGGGA CCCAGGGCTG
    AGCTCCCCGG AGCTCTGTCA GCTCCACCTG TCCATCTGTC ACGAGTGTCT GGAGCTCGAG
    AACAGTACCA TTGACTCGGT GAGGTCTGCG TCTGCGGAGA ACATTCCAGA CCTTCCCTGT
    GATCACAGCG GCGTGGAGGG TGCTGCGGGC GAACTCTGCC CCGAACGGAA AGGGAAGAGA
    GTCAACATTT CGGGAAAGGC GCCCAATATC TTGCTGTATG TGGGCTCTGG TTCCGAGGAA
    GCCCTGGGCC GGCTCCAGCA GGTGCGATCT GTCCTGACTG ACTGTGTGGA CACGGACAGC
    TACACTCTCT ACCACCTGCT GGAAGACAGC GCTCTGAGGG ACCCGTGGTC TGACAACTGC
    CTCCTGCTAG TTATTGCCAG CAGGGACCCC ATCCCCAAAG ACATACAGCA CAAGTTCATG
    GCCTATCTTT CTCAGGGAGG GAAGGTGCTG GGCCTGTCTT CCCCCTTCAC GTTGGGTGGC
    TTTCGGGTGA CCAGGAGAGA TGTGCTACGG AACACAGTCC AGAACTTGGT TTTCTCTAAG
    GCTGATGGGA CTGAGGTACG GCTCAGTGTC CTGAGCAGCG GCTACGTTTA TGAAGAAGGC
    CCGAGCTTGG GCAGGCTCCA GGGCCACCTG GAGAATGAGG ACAAGGATAA AATGATTGTG
    CACGTGCCTT TTGGAACCCT CGGAGGGGAA GCCGTTCTCT GCCAGGTGCA CCTGGAGCTC
    CCGCCCGGCG CCTCCCTGGT GCAGACTGCA GACGACTTTA ACGTGCTCAA ATCCAGCAAT
    GTGAGAAGAC ACGAGGTCCT TAAGGAGATC CTGACTGCCC TCGGTCTGAG CTGTGACGCA
    CCACAGGTTC CTGCCTTAAC GCCCCTGTAC TTACTGCTGG CCGCTGAGGA AACCCAGGAT
    CCGTTCATGC AGTGGCTCGG GAGGCACACA GATCCTGAAG GAATCATAAA ATCCAGCAAG
    CTCTCCCTTC AGTTTGTTTC CTCCTACACA TCTGAAGCGG AGATCACCCC GTCTTCCATG
    CCTGTGGTCA CTGACCCAGA AGCCTTTTCA TCAGAGCACT TCAGCCTAGA GACCTATCGA
    CAGAATCTGC AGACCACGCG CCTTGGGAAA GTGATTCTGT TTGCAGAAGT GACCTCCACC
    ACCATGAGTC TCCTGGATGG GTTGATGTTT GAGATGCCGC AGGAAATGGG CTTAATTGCC
    ATTGCAGTTC GGCAGACCCA GGGCAAAGGA AGGGGCCCGA ATGCCTGGCT GAGCCCCGTG
    GGATGTGCTC TTTCCACTCT GCTGGTCTTC ATCCCCCTGA GGTCACAACT GGGACAGAGG
    ATTCCATTTG TCCAGCATTT GATGTCCTTG GCTGTGGTGG AGGCAGTGCG GTCTATCCCA
    GGGTATGAGG ATATCAACTT GCGAGTGAAG TGGCCCAACG ATATTTATTA CAGCGATCTC
    ATGAAGATCG GTGGGGTTCT GGTTAATTCA ACACTTATGG GAGAAACATT TTATATCCTC
    ATCGGCTGTG GATTCAATGT GACGAACAGT AATCCTACCA TTTGTATCAA CGACCTCATC
    GAAGAACACA ATAAGCAGCA CGGGGCGGGC CTGAAGCCCC TGCGAGCGGA CTGTCTCATA
    GCCAGAGCCG TGACCGTGCT GGAGAAACTG ATCGACAGGT TTCAGGACCA AGGGCCCGAC
    GGCGTTCTTC CCCTCTATTA TAAATACTGG GTGCACGGTG GTCAGCAAGT CCGCCTGGGC
    AGCACGGAGG GGCCACAGGC ATCTATTGTG GGCCTTGATG ACTCCGGGTT CCTGCAGGTC
    CACCAAGAGG ATGGTGGGGT TGTGACGGTG CATCCAGATG GCAACTCCTT CGACATGCTG
    AGAAACCTCA TCGTGCCCAA GCGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001346974.1
    CDS35..2644
    Translation

    Target ORF information:

    RefSeq Version NM_001360045.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001360045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCCG GCCGGCCGCG 
    CTGGTGCTGT CCACGGTGCG GCGGCTGCGT GCTTCGCGCT GCTCCTTCAC CTTCTGCCGC
    GCGGCCGCGC AGCCCAGGGA CGCGCGCGTG TGCCTGAGCC AGGGCGGCCG CGTCTTCTGC
    GTCGGCGAGA GCCAGTCTGT TGATGACTTG AACAAATGGG CCCTGTTTCT CGTGTCTCCC
    TTCATACTTG AGGCAGAACA TATAGCATTT GTGACAGAGA GCATTTGGAT ACAAGGTACC
    AGTTTACAAG AGCCATCATC TTCAGAAACC GTTGTAAAAT GGTCTGACTG CTGTCTGCCG
    TTAGCCTGCC GACCCGGGGA CCCCTACCAG TTAATTGCTA AGGCAAGCGT GGACAACTTC
    AGCAAGCTTG GGGTGGCCTT CATGGAGGAC AGGCTCCAGA TGGACAACGG CCTGATAGCC
    CAGAAGATCG TTTCAGTCCA CTTAAAGGAC CCTGCTCTGA AGGAACTCGG CAAAGCCTCC
    GACAAGCAAG TGCAGGGCCC GCCCCCGGGC CCAGAAGCTT CCCCCGAGGC CCAGCCTGCA
    CAAGGTGTCA TGGAGCATGC TGGCCAAGGT GATTGCAAGG CCGCCGGTGA AGGCCCTTCC
    CCACGGAGGA GGGGCTGTGC CCCTGAAAGT GAGCCTGCTG CAGATGGGGA CCCAGGGCTG
    AGCTCCCCGG AGCTCTGTCA GCTCCACCTG TCCATCTGTC ACGAGTGTCT GGAGCTCGAG
    AACAGTACCA TTGACTCGGT GAGGTCTGCG TCTGCGGAGA ACATTCCAGA CCTTCCCTGT
    GATCACAGCG GCGTGGAGGG TGCTGCGGGC GAACTCTGCC CCGAACGGAA AGGGAAGAGA
    GTCAACATTT CGGGAAAGGC GCCCAATATC TTGCTGTATG TGGGCTCTGG TTCCGAGGAA
    GCCCTGGGCC GGCTCCAGCA GGTGCGATCT GTCCTGACTG ACTGTGTGGA CACGGACAGC
    TACACTCTCT ACCACCTGCT GGAAGACAGC GCTCTGAGGG ACCCGTGGTC TGACAACTGC
    CTCCTGCTAG TTATTGCCAG CAGGGACCCC ATCCCCAAAG ACATACAGCA CAAGTTCATG
    GCCTATCTTT CTCAGGGAGG GAAGGTGCTG GGCCTGTCTT CCCCCTTCAC GTTGGGTGGC
    TTTCGGGTGA CCAGGAGAGA TGTGCTACGG AACACAGTCC AGAACTTGGT TTTCTCTAAG
    GCTGATGGGA CTGAGGTACG GCTCAGTGTC CTGAGCAGCG GCTACGTTTA TGAAGAAGGC
    CCGAGCTTGG GCAGGCTCCA GGGCCACCTG GAGAATGAGG ACAAGGATAA AATGATTGTG
    CACGTGCCTT TTGGAACCCT CGGAGGGGAA GCCGTTCTCT GCCAGGTGCA CCTGGAGCTC
    CCGCCCGGCG CCTCCCTGGT GCAGACTGCA GACGACTTTA ACGTGCTCAA ATCCAGCAAT
    GTGAGAAGAC ACGAGGTCCT TAAGGAGATC CTGACTGCCC TCGGTCTGAG CTGTGACGCA
    CCACAGGTTC CTGCCTTAAC GCCCCTGTAC TTACTGCTGG CCGCTGAGGA AACCCAGGAT
    CCGTTCATGC AGTGGCTCGG GAGGCACACA GATCCTGAAG GAATCATAAA ATCCAGCAAG
    CTCTCCCTTC AGTTTGTTTC CTCCTACACA TCTGAAGCGG AGATCACCCC GTCTTCCATG
    CCTGTGGTCA CTGACCCAGA AGCCTTTTCA TCAGAGCACT TCAGCCTAGA GACCTATCGA
    CAGAATCTGC AGACCACGCG CCTTGGGAAA GTGATTCTGT TTGCAGAAGT GACCTCCACC
    ACCATGAGTC TCCTGGATGG GTTGATGTTT GAGATGCCGC AGGAAATGGG CTTAATTGCC
    ATTGCAGTTC GGCAGACCCA GGGCAAAGGA AGGGGCCCGA ATGCCTGGCT GAGCCCCGTG
    GGATGTGCTC TTTCCACTCT GCTGGTCTTC ATCCCCCTGA GGTCACAACT GGGACAGAGG
    ATTCCATTTG TCCAGCATTT GATGTCCTTG GCTGTGGTGG AGGCAGTGCG GTCTATCCCA
    GGGTATGAGG ATATCAACTT GCGAGTGAAG TGGCCCAACG ATATTTATTA CAGCGATCTC
    ATGAAGATCG GTGGGGTTCT GGTTAATTCA ACACTTATGG GAGAAACATT TTATATCCTC
    ATCGGCTGTG GATTCAATGT GACGAACAGT AATCCTACCA TTTGTATCAA CGACCTCATC
    GAAGAACACA ATAAGCAGCA CGGGGCGGGC CTGAAGCCCC TGCGAGCGGA CTGTCTCATA
    GCCAGAGCCG TGACCGTGCT GGAGAAACTG ATCGACAGGT TTCAGGACCA AGGGCCCGAC
    GGCGTTCTTC CCCTCTATTA TAAATACTGG GTGCACGGTG GTCAGCAAGT CCGCCTGGGC
    AGCACGGAGG GGCCACAGGC ATCTATTGTG GGCCTTGATG ACTCCGGGTT CCTGCAGGTC
    CACCAAGAGG ATGGTGGGGT TGTGACGGTG CATCCAGATG GCAACTCCTT CGACATGCTG
    AGAAACCTCA TCGTGCCCAA GCGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu10948
    Clone ID Related Accession (Same CDS sequence) XM_006522855.1 , XM_006522856.2 , XM_006522856.1 , NM_139145.4 , XM_030248919.1 , XM_006522855.2 , XM_030248920.1 , NM_139145.5
    Accession Version XM_030248919.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030104779.1
    CDS855..3023
    Translation

    Target ORF information:

    RefSeq Version XM_030248919.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030248919.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu10948
    Clone ID Related Accession (Same CDS sequence) XM_006522855.1 , XM_006522856.2 , XM_006522856.1 , NM_139145.4 , XM_030248919.1 , XM_006522855.2 , XM_030248920.1 , NM_139145.5
    Accession Version XM_030248920.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030104780.1
    CDS287..2455
    Translation

    Target ORF information:

    RefSeq Version XM_030248920.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030248920.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGACA GGCTCCAGAT GGACAACGGC CTGATAGCCC AGAAGATCGT TTCAGTCCAC 
    TTAAAGGACC CTGCTCTGAA GGAACTCGGC AAAGCCTCCG ACAAGCAAGT GCAGGGCCCG
    CCCCCGGGCC CAGAAGCTTC CCCCGAGGCC CAGCCTGCAC AAGGTGTCAT GGAGCATGCT
    GGCCAAGGTG ATTGCAAGGC CGCCGGTGAA GGCCCTTCCC CACGGAGGAG GGGCTGTGCC
    CCTGAAAGTG AGCCTGCTGC AGATGGGGAC CCAGGGCTGA GCTCCCCGGA GCTCTGTCAG
    CTCCACCTGT CCATCTGTCA CGAGTGTCTG GAGCTCGAGA ACAGTACCAT TGACTCGGTG
    AGGTCTGCGT CTGCGGAGAA CATTCCAGAC CTTCCCTGTG ATCACAGCGG CGTGGAGGGT
    GCTGCGGGCG AACTCTGCCC CGAACGGAAA GGGAAGAGAG TCAACATTTC GGGAAAGGCG
    CCCAATATCT TGCTGTATGT GGGCTCTGGT TCCGAGGAAG CCCTGGGCCG GCTCCAGCAG
    GTGCGATCTG TCCTGACTGA CTGTGTGGAC ACGGACAGCT ACACTCTCTA CCACCTGCTG
    GAAGACAGCG CTCTGAGGGA CCCGTGGTCT GACAACTGCC TCCTGCTAGT TATTGCCAGC
    AGGGACCCCA TCCCCAAAGA CATACAGCAC AAGTTCATGG CCTATCTTTC TCAGGGAGGG
    AAGGTGCTGG GCCTGTCTTC CCCCTTCACG TTGGGTGGCT TTCGGGTGAC CAGGAGAGAT
    GTGCTACGGA ACACAGTCCA GAACTTGGTT TTCTCTAAGG CTGATGGGAC TGAGGTACGG
    CTCAGTGTCC TGAGCAGCGG CTACGTTTAT GAAGAAGGCC CGAGCTTGGG CAGGCTCCAG
    GGCCACCTGG AGAATGAGGA CAAGGATAAA ATGATTGTGC ACGTGCCTTT TGGAACCCTC
    GGAGGGGAAG CCGTTCTCTG CCAGGTGCAC CTGGAGCTCC CGCCCGGCGC CTCCCTGGTG
    CAGACTGCAG ACGACTTTAA CGTGCTCAAA TCCAGCAATG TGAGAAGACA CGAGGTCCTT
    AAGGAGATCC TGACTGCCCT CGGTCTGAGC TGTGACGCAC CACAGGTTCC TGCCTTAACG
    CCCCTGTACT TACTGCTGGC CGCTGAGGAA ACCCAGGATC CGTTCATGCA GTGGCTCGGG
    AGGCACACAG ATCCTGAAGG AATCATAAAA TCCAGCAAGC TCTCCCTTCA GTTTGTTTCC
    TCCTACACAT CTGAAGCGGA GATCACCCCG TCTTCCATGC CTGTGGTCAC TGACCCAGAA
    GCCTTTTCAT CAGAGCACTT CAGCCTAGAG ACCTATCGAC AGAATCTGCA GACCACGCGC
    CTTGGGAAAG TGATTCTGTT TGCAGAAGTG ACCTCCACCA CCATGAGTCT CCTGGATGGG
    TTGATGTTTG AGATGCCGCA GGAAATGGGC TTAATTGCCA TTGCAGTTCG GCAGACCCAG
    GGCAAAGGAA GGGGCCCGAA TGCCTGGCTG AGCCCCGTGG GATGTGCTCT TTCCACTCTG
    CTGGTCTTCA TCCCCCTGAG GTCACAACTG GGACAGAGGA TTCCATTTGT CCAGCATTTG
    ATGTCCTTGG CTGTGGTGGA GGCAGTGCGG TCTATCCCAG GGTATGAGGA TATCAACTTG
    CGAGTGAAGT GGCCCAACGA TATTTATTAC AGCGATCTCA TGAAGATCGG TGGGGTTCTG
    GTTAATTCAA CACTTATGGG AGAAACATTT TATATCCTCA TCGGCTGTGG ATTCAATGTG
    ACGAACAGTA ATCCTACCAT TTGTATCAAC GACCTCATCG AAGAACACAA TAAGCAGCAC
    GGGGCGGGCC TGAAGCCCCT GCGAGCGGAC TGTCTCATAG CCAGAGCCGT GACCGTGCTG
    GAGAAACTGA TCGACAGGTT TCAGGACCAA GGGCCCGACG GCGTTCTTCC CCTCTATTAT
    AAATACTGGG TGCACGGTGG TCAGCAAGTC CGCCTGGGCA GCACGGAGGG GCCACAGGCA
    TCTATTGTGG GCCTTGATGA CTCCGGGTTC CTGCAGGTCC ACCAAGAGGA TGGTGGGGTT
    GTGACGGTGC ATCCAGATGG CAACTCCTTC GACATGCTGA GAAACCTCAT CGTGCCCAAG
    CGACAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52627
    Clone ID Related Accession (Same CDS sequence) XM_006522851.3 , XM_006522851.4
    Accession Version XM_006522851.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522851.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTGTCTGG CACGCGGTGC TGCGCGAATG GCAAGCCTGG CTTTGCAGGG GACAGGGAGC 
    TGGGGATCCA GATTGCAGGG GCAGATGGCA GACTGGCTGC GCGTCCCCGA CGCAAGGGCA
    GCTTTGCGCA GTGCGCATCC CCGCGCGCTG CTCGCCGCGG CCGCCAGGGG GCGCAGCGGG
    ATCTCAGTGC GATCACCCTG CGGAGCGGGC AGCCGCGCTC AGGGCTGCGA GTCTGTTGAT
    GACTTGAACA AATGGGCCCT GTTTCTCGTG TCTCCCTTCA TACTTGAGGC AGAACATATA
    GCATTTGTGA CAGAGAGCAT TTGGATACAA GGTACCAGTT TACAAGAGCC ATCATCTTCA
    GAAACCGTTG TAAAATGGTC TGACTGCTGT CTGCCGTTAG CCTGCCGACC CGGGGACCCC
    TACCAGTTAA TTGCTAAGGC AAGCGTGGAC AACTTCAGCA AGCTTGGGGT GGCCTTCATG
    GAGGACAGGC TCCAGATGGA CAACGGCCTG ATAGCCCAGA AGATCGTTTC AGTCCACTTA
    AAGGACCCTG CTCTGAAGGA ACTCGGCAAA GCCTCCGACA AGCAAGTGCA GGGCCCGCCC
    CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG CCTGCACAAG GTGTCATGGA GCATGCTGGC
    CAAGGTGATT GCAAGGCCGC CGGTGAAGGC CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT
    GAAAGTGAGC CTGCTGCAGA TGGGGACCCA GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC
    CACCTGTCCA TCTGTCACGA GTGTCTGGAG CTCGAGAACA GTACCATTGA CTCGGTGAGG
    TCTGCGTCTG CGGAGAACAT TCCAGACCTT CCCTGTGATC ACAGCGGCGT GGAGGGTGCT
    GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG AAGAGAGTCA ACATTTCGGG AAAGGCGCCC
    AATATCTTGC TGTATGTGGG CTCTGGTTCC GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG
    CGATCTGTCC TGACTGACTG TGTGGACACG GACAGCTACA CTCTCTACCA CCTGCTGGAA
    GACAGCGCTC TGAGGGACCC GTGGTCTGAC AACTGCCTCC TGCTAGTTAT TGCCAGCAGG
    GACCCCATCC CCAAAGACAT ACAGCACAAG TTCATGGCCT ATCTTTCTCA GGGAGGGAAG
    GTGCTGGGCC TGTCTTCCCC CTTCACGTTG GGTGGCTTTC GGGTGACCAG GAGAGATGTG
    CTACGGAACA CAGTCCAGAA CTTGGTTTTC TCTAAGGCTG ATGGGACTGA GGTACGGCTC
    AGTGTCCTGA GCAGCGGCTA CGTTTATGAA GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC
    CACCTGGAGA ATGAGGACAA GGATAAAATG ATTGTGCACG TGCCTTTTGG AACCCTCGGA
    GGGGAAGCCG TTCTCTGCCA GGTGCACCTG GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG
    ACTGCAGACG ACTTTAACGT GCTCAAATCC AGCAATGTGA GAAGACACGA GGTCCTTAAG
    GAGATCCTGA CTGCCCTCGG TCTGAGCTGT GACGCACCAC AGGTTCCTGC CTTAACGCCC
    CTGTACTTAC TGCTGGCCGC TGAGGAAACC CAGGATCCGT TCATGCAGTG GCTCGGGAGG
    CACACAGATC CTGAAGGAAT CATAAAATCC AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC
    TACACATCTG AAGCGGAGAT CACCCCGTCT TCCATGCCTG TGGTCACTGA CCCAGAAGCC
    TTTTCATCAG AGCACTTCAG CCTAGAGACC TATCGACAGA ATCTGCAGAC CACGCGCCTT
    GGGAAAGTGA TTCTGTTTGC AGAAGTGACC TCCACCACCA TGAGTCTCCT GGATGGGTTG
    ATGTTTGAGA TGCCGCAGGA AATGGGCTTA ATTGCCATTG CAGTTCGGCA GACCCAGGGC
    AAAGGAAGGG GCCCGAATGC CTGGCTGAGC CCCGTGGGAT GTGCTCTTTC CACTCTGCTG
    GTCTTCATCC CCCTGAGGTC ACAACTGGGA CAGAGGATTC CATTTGTCCA GCATTTGATG
    TCCTTGGCTG TGGTGGAGGC AGTGCGGTCT ATCCCAGGGT ATGAGGATAT CAACTTGCGA
    GTGAAGTGGC CCAACGATAT TTATTACAGC GATCTCATGA AGATCGGTGG GGTTCTGGTT
    AATTCAACAC TTATGGGAGA AACATTTTAT ATCCTCATCG GCTGTGGATT CAATGTGACG
    AACAGTAATC CTACCATTTG TATCAACGAC CTCATCGAAG AACACAATAA GCAGCACGGG
    GCGGGCCTGA AGCCCCTGCG AGCGGACTGT CTCATAGCCA GAGCCGTGAC CGTGCTGGAG
    AAACTGATCG ACAGGTTTCA GGACCAAGGG CCCGACGGCG TTCTTCCCCT CTATTATAAA
    TACTGGGTGC ACGGTGGTCA GCAAGTCCGC CTGGGCAGCA CGGAGGGGCC ACAGGCATCT
    ATTGTGGGCC TTGATGACTC CGGGTTCCTG CAGGTCCACC AAGAGGATGG TGGGGTTGTG
    ACGGTGCATC CAGATGGCAA CTCCTTCGAC ATGCTGAGAA ACCTCATCGT GCCCAAGCGA
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522914.1
    CDS1492..4137
    Translation

    Target ORF information:

    RefSeq Version XM_006522851.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522851.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTGTCTGG CACGCGGTGC TGCGCGAATG GCAAGCCTGG CTTTGCAGGG GACAGGGAGC 
    TGGGGATCCA GATTGCAGGG GCAGATGGCA GACTGGCTGC GCGTCCCCGA CGCAAGGGCA
    GCTTTGCGCA GTGCGCATCC CCGCGCGCTG CTCGCCGCGG CCGCCAGGGG GCGCAGCGGG
    ATCTCAGTGC GATCACCCTG CGGAGCGGGC AGCCGCGCTC AGGGCTGCGA GTCTGTTGAT
    GACTTGAACA AATGGGCCCT GTTTCTCGTG TCTCCCTTCA TACTTGAGGC AGAACATATA
    GCATTTGTGA CAGAGAGCAT TTGGATACAA GGTACCAGTT TACAAGAGCC ATCATCTTCA
    GAAACCGTTG TAAAATGGTC TGACTGCTGT CTGCCGTTAG CCTGCCGACC CGGGGACCCC
    TACCAGTTAA TTGCTAAGGC AAGCGTGGAC AACTTCAGCA AGCTTGGGGT GGCCTTCATG
    GAGGACAGGC TCCAGATGGA CAACGGCCTG ATAGCCCAGA AGATCGTTTC AGTCCACTTA
    AAGGACCCTG CTCTGAAGGA ACTCGGCAAA GCCTCCGACA AGCAAGTGCA GGGCCCGCCC
    CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG CCTGCACAAG GTGTCATGGA GCATGCTGGC
    CAAGGTGATT GCAAGGCCGC CGGTGAAGGC CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT
    GAAAGTGAGC CTGCTGCAGA TGGGGACCCA GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC
    CACCTGTCCA TCTGTCACGA GTGTCTGGAG CTCGAGAACA GTACCATTGA CTCGGTGAGG
    TCTGCGTCTG CGGAGAACAT TCCAGACCTT CCCTGTGATC ACAGCGGCGT GGAGGGTGCT
    GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG AAGAGAGTCA ACATTTCGGG AAAGGCGCCC
    AATATCTTGC TGTATGTGGG CTCTGGTTCC GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG
    CGATCTGTCC TGACTGACTG TGTGGACACG GACAGCTACA CTCTCTACCA CCTGCTGGAA
    GACAGCGCTC TGAGGGACCC GTGGTCTGAC AACTGCCTCC TGCTAGTTAT TGCCAGCAGG
    GACCCCATCC CCAAAGACAT ACAGCACAAG TTCATGGCCT ATCTTTCTCA GGGAGGGAAG
    GTGCTGGGCC TGTCTTCCCC CTTCACGTTG GGTGGCTTTC GGGTGACCAG GAGAGATGTG
    CTACGGAACA CAGTCCAGAA CTTGGTTTTC TCTAAGGCTG ATGGGACTGA GGTACGGCTC
    AGTGTCCTGA GCAGCGGCTA CGTTTATGAA GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC
    CACCTGGAGA ATGAGGACAA GGATAAAATG ATTGTGCACG TGCCTTTTGG AACCCTCGGA
    GGGGAAGCCG TTCTCTGCCA GGTGCACCTG GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG
    ACTGCAGACG ACTTTAACGT GCTCAAATCC AGCAATGTGA GAAGACACGA GGTCCTTAAG
    GAGATCCTGA CTGCCCTCGG TCTGAGCTGT GACGCACCAC AGGTTCCTGC CTTAACGCCC
    CTGTACTTAC TGCTGGCCGC TGAGGAAACC CAGGATCCGT TCATGCAGTG GCTCGGGAGG
    CACACAGATC CTGAAGGAAT CATAAAATCC AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC
    TACACATCTG AAGCGGAGAT CACCCCGTCT TCCATGCCTG TGGTCACTGA CCCAGAAGCC
    TTTTCATCAG AGCACTTCAG CCTAGAGACC TATCGACAGA ATCTGCAGAC CACGCGCCTT
    GGGAAAGTGA TTCTGTTTGC AGAAGTGACC TCCACCACCA TGAGTCTCCT GGATGGGTTG
    ATGTTTGAGA TGCCGCAGGA AATGGGCTTA ATTGCCATTG CAGTTCGGCA GACCCAGGGC
    AAAGGAAGGG GCCCGAATGC CTGGCTGAGC CCCGTGGGAT GTGCTCTTTC CACTCTGCTG
    GTCTTCATCC CCCTGAGGTC ACAACTGGGA CAGAGGATTC CATTTGTCCA GCATTTGATG
    TCCTTGGCTG TGGTGGAGGC AGTGCGGTCT ATCCCAGGGT ATGAGGATAT CAACTTGCGA
    GTGAAGTGGC CCAACGATAT TTATTACAGC GATCTCATGA AGATCGGTGG GGTTCTGGTT
    AATTCAACAC TTATGGGAGA AACATTTTAT ATCCTCATCG GCTGTGGATT CAATGTGACG
    AACAGTAATC CTACCATTTG TATCAACGAC CTCATCGAAG AACACAATAA GCAGCACGGG
    GCGGGCCTGA AGCCCCTGCG AGCGGACTGT CTCATAGCCA GAGCCGTGAC CGTGCTGGAG
    AAACTGATCG ACAGGTTTCA GGACCAAGGG CCCGACGGCG TTCTTCCCCT CTATTATAAA
    TACTGGGTGC ACGGTGGTCA GCAAGTCCGC CTGGGCAGCA CGGAGGGGCC ACAGGCATCT
    ATTGTGGGCC TTGATGACTC CGGGTTCCTG CAGGTCCACC AAGAGGATGG TGGGGTTGTG
    ACGGTGCATC CAGATGGCAA CTCCTTCGAC ATGCTGAGAA ACCTCATCGT GCCCAAGCGA
    CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52628
    Clone ID Related Accession (Same CDS sequence) XM_006522852.3 , XM_006522852.4
    Accession Version XM_006522852.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522852.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTGTCTGG CACGCGGTGC TGCGCGAATG GCAAGCCTGG CTTTGCAGGG GACAGGGAGC 
    TGGGGATCCA GATTGCAGGG GCAGATGGCA GACTGGCTGC GCGTCCCCGA CGCAAGGGCA
    GCTTTGCGCA GTGCGCATCC CCGCGCGCTG CTCGCCGCGG CCGCCAGGGG GCGCAGCGGG
    ATCTCAGTGC GATCACCCTG CGGAGCGGGC AGCCGCGCTC AGGGCTGCGA GGTTGTAAAA
    TGGTCTGACT GCTGTCTGCC GTTAGCCTGC CGACCCGGGG ACCCCTACCA GTTAATTGCT
    AAGGCAAGCG TGGACAACTT CAGCAAGCTT GGGGTGGCCT TCATGGAGGA CAGGCTCCAG
    ATGGACAACG GCCTGATAGC CCAGAAGATC GTTTCAGTCC ACTTAAAGGA CCCTGCTCTG
    AAGGAACTCG GCAAAGCCTC CGACAAGCAA GTGCAGGGCC CGCCCCCGGG CCCAGAAGCT
    TCCCCCGAGG CCCAGCCTGC ACAAGGTGTC ATGGAGCATG CTGGCCAAGG TGATTGCAAG
    GCCGCCGGTG AAGGCCCTTC CCCACGGAGG AGGGGCTGTG CCCCTGAAAG TGAGCCTGCT
    GCAGATGGGG ACCCAGGGCT GAGCTCCCCG GAGCTCTGTC AGCTCCACCT GTCCATCTGT
    CACGAGTGTC TGGAGCTCGA GAACAGTACC ATTGACTCGG TGAGGTCTGC GTCTGCGGAG
    AACATTCCAG ACCTTCCCTG TGATCACAGC GGCGTGGAGG GTGCTGCGGG CGAACTCTGC
    CCCGAACGGA AAGGGAAGAG AGTCAACATT TCGGGAAAGG CGCCCAATAT CTTGCTGTAT
    GTGGGCTCTG GTTCCGAGGA AGCCCTGGGC CGGCTCCAGC AGGTGCGATC TGTCCTGACT
    GACTGTGTGG ACACGGACAG CTACACTCTC TACCACCTGC TGGAAGACAG CGCTCTGAGG
    GACCCGTGGT CTGACAACTG CCTCCTGCTA GTTATTGCCA GCAGGGACCC CATCCCCAAA
    GACATACAGC ACAAGTTCAT GGCCTATCTT TCTCAGGGAG GGAAGGTGCT GGGCCTGTCT
    TCCCCCTTCA CGTTGGGTGG CTTTCGGGTG ACCAGGAGAG ATGTGCTACG GAACACAGTC
    CAGAACTTGG TTTTCTCTAA GGCTGATGGG ACTGAGGTAC GGCTCAGTGT CCTGAGCAGC
    GGCTACGTTT ATGAAGAAGG CCCGAGCTTG GGCAGGCTCC AGGGCCACCT GGAGAATGAG
    GACAAGGATA AAATGATTGT GCACGTGCCT TTTGGAACCC TCGGAGGGGA AGCCGTTCTC
    TGCCAGGTGC ACCTGGAGCT CCCGCCCGGC GCCTCCCTGG TGCAGACTGC AGACGACTTT
    AACGTGCTCA AATCCAGCAA TGTGAGAAGA CACGAGGTCC TTAAGGAGAT CCTGACTGCC
    CTCGGTCTGA GCTGTGACGC ACCACAGGTT CCTGCCTTAA CGCCCCTGTA CTTACTGCTG
    GCCGCTGAGG AAACCCAGGA TCCGTTCATG CAGTGGCTCG GGAGGCACAC AGATCCTGAA
    GGAATCATAA AATCCAGCAA GCTCTCCCTT CAGTTTGTTT CCTCCTACAC ATCTGAAGCG
    GAGATCACCC CGTCTTCCAT GCCTGTGGTC ACTGACCCAG AAGCCTTTTC ATCAGAGCAC
    TTCAGCCTAG AGACCTATCG ACAGAATCTG CAGACCACGC GCCTTGGGAA AGTGATTCTG
    TTTGCAGAAG TGACCTCCAC CACCATGAGT CTCCTGGATG GGTTGATGTT TGAGATGCCG
    CAGGAAATGG GCTTAATTGC CATTGCAGTT CGGCAGACCC AGGGCAAAGG AAGGGGCCCG
    AATGCCTGGC TGAGCCCCGT GGGATGTGCT CTTTCCACTC TGCTGGTCTT CATCCCCCTG
    AGGTCACAAC TGGGACAGAG GATTCCATTT GTCCAGCATT TGATGTCCTT GGCTGTGGTG
    GAGGCAGTGC GGTCTATCCC AGGGTATGAG GATATCAACT TGCGAGTGAA GTGGCCCAAC
    GATATTTATT ACAGCGATCT CATGAAGATC GGTGGGGTTC TGGTTAATTC AACACTTATG
    GGAGAAACAT TTTATATCCT CATCGGCTGT GGATTCAATG TGACGAACAG TAATCCTACC
    ATTTGTATCA ACGACCTCAT CGAAGAACAC AATAAGCAGC ACGGGGCGGG CCTGAAGCCC
    CTGCGAGCGG ACTGTCTCAT AGCCAGAGCC GTGACCGTGC TGGAGAAACT GATCGACAGG
    TTTCAGGACC AAGGGCCCGA CGGCGTTCTT CCCCTCTATT ATAAATACTG GGTGCACGGT
    GGTCAGCAAG TCCGCCTGGG CAGCACGGAG GGGCCACAGG CATCTATTGT GGGCCTTGAT
    GACTCCGGGT TCCTGCAGGT CCACCAAGAG GATGGTGGGG TTGTGACGGT GCATCCAGAT
    GGCAACTCCT TCGACATGCT GAGAAACCTC ATCGTGCCCA AGCGACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522915.1
    CDS1492..4002
    Translation

    Target ORF information:

    RefSeq Version XM_006522852.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522852.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTGTCTGG CACGCGGTGC TGCGCGAATG GCAAGCCTGG CTTTGCAGGG GACAGGGAGC 
    TGGGGATCCA GATTGCAGGG GCAGATGGCA GACTGGCTGC GCGTCCCCGA CGCAAGGGCA
    GCTTTGCGCA GTGCGCATCC CCGCGCGCTG CTCGCCGCGG CCGCCAGGGG GCGCAGCGGG
    ATCTCAGTGC GATCACCCTG CGGAGCGGGC AGCCGCGCTC AGGGCTGCGA GGTTGTAAAA
    TGGTCTGACT GCTGTCTGCC GTTAGCCTGC CGACCCGGGG ACCCCTACCA GTTAATTGCT
    AAGGCAAGCG TGGACAACTT CAGCAAGCTT GGGGTGGCCT TCATGGAGGA CAGGCTCCAG
    ATGGACAACG GCCTGATAGC CCAGAAGATC GTTTCAGTCC ACTTAAAGGA CCCTGCTCTG
    AAGGAACTCG GCAAAGCCTC CGACAAGCAA GTGCAGGGCC CGCCCCCGGG CCCAGAAGCT
    TCCCCCGAGG CCCAGCCTGC ACAAGGTGTC ATGGAGCATG CTGGCCAAGG TGATTGCAAG
    GCCGCCGGTG AAGGCCCTTC CCCACGGAGG AGGGGCTGTG CCCCTGAAAG TGAGCCTGCT
    GCAGATGGGG ACCCAGGGCT GAGCTCCCCG GAGCTCTGTC AGCTCCACCT GTCCATCTGT
    CACGAGTGTC TGGAGCTCGA GAACAGTACC ATTGACTCGG TGAGGTCTGC GTCTGCGGAG
    AACATTCCAG ACCTTCCCTG TGATCACAGC GGCGTGGAGG GTGCTGCGGG CGAACTCTGC
    CCCGAACGGA AAGGGAAGAG AGTCAACATT TCGGGAAAGG CGCCCAATAT CTTGCTGTAT
    GTGGGCTCTG GTTCCGAGGA AGCCCTGGGC CGGCTCCAGC AGGTGCGATC TGTCCTGACT
    GACTGTGTGG ACACGGACAG CTACACTCTC TACCACCTGC TGGAAGACAG CGCTCTGAGG
    GACCCGTGGT CTGACAACTG CCTCCTGCTA GTTATTGCCA GCAGGGACCC CATCCCCAAA
    GACATACAGC ACAAGTTCAT GGCCTATCTT TCTCAGGGAG GGAAGGTGCT GGGCCTGTCT
    TCCCCCTTCA CGTTGGGTGG CTTTCGGGTG ACCAGGAGAG ATGTGCTACG GAACACAGTC
    CAGAACTTGG TTTTCTCTAA GGCTGATGGG ACTGAGGTAC GGCTCAGTGT CCTGAGCAGC
    GGCTACGTTT ATGAAGAAGG CCCGAGCTTG GGCAGGCTCC AGGGCCACCT GGAGAATGAG
    GACAAGGATA AAATGATTGT GCACGTGCCT TTTGGAACCC TCGGAGGGGA AGCCGTTCTC
    TGCCAGGTGC ACCTGGAGCT CCCGCCCGGC GCCTCCCTGG TGCAGACTGC AGACGACTTT
    AACGTGCTCA AATCCAGCAA TGTGAGAAGA CACGAGGTCC TTAAGGAGAT CCTGACTGCC
    CTCGGTCTGA GCTGTGACGC ACCACAGGTT CCTGCCTTAA CGCCCCTGTA CTTACTGCTG
    GCCGCTGAGG AAACCCAGGA TCCGTTCATG CAGTGGCTCG GGAGGCACAC AGATCCTGAA
    GGAATCATAA AATCCAGCAA GCTCTCCCTT CAGTTTGTTT CCTCCTACAC ATCTGAAGCG
    GAGATCACCC CGTCTTCCAT GCCTGTGGTC ACTGACCCAG AAGCCTTTTC ATCAGAGCAC
    TTCAGCCTAG AGACCTATCG ACAGAATCTG CAGACCACGC GCCTTGGGAA AGTGATTCTG
    TTTGCAGAAG TGACCTCCAC CACCATGAGT CTCCTGGATG GGTTGATGTT TGAGATGCCG
    CAGGAAATGG GCTTAATTGC CATTGCAGTT CGGCAGACCC AGGGCAAAGG AAGGGGCCCG
    AATGCCTGGC TGAGCCCCGT GGGATGTGCT CTTTCCACTC TGCTGGTCTT CATCCCCCTG
    AGGTCACAAC TGGGACAGAG GATTCCATTT GTCCAGCATT TGATGTCCTT GGCTGTGGTG
    GAGGCAGTGC GGTCTATCCC AGGGTATGAG GATATCAACT TGCGAGTGAA GTGGCCCAAC
    GATATTTATT ACAGCGATCT CATGAAGATC GGTGGGGTTC TGGTTAATTC AACACTTATG
    GGAGAAACAT TTTATATCCT CATCGGCTGT GGATTCAATG TGACGAACAG TAATCCTACC
    ATTTGTATCA ACGACCTCAT CGAAGAACAC AATAAGCAGC ACGGGGCGGG CCTGAAGCCC
    CTGCGAGCGG ACTGTCTCAT AGCCAGAGCC GTGACCGTGC TGGAGAAACT GATCGACAGG
    TTTCAGGACC AAGGGCCCGA CGGCGTTCTT CCCCTCTATT ATAAATACTG GGTGCACGGT
    GGTCAGCAAG TCCGCCTGGG CAGCACGGAG GGGCCACAGG CATCTATTGT GGGCCTTGAT
    GACTCCGGGT TCCTGCAGGT CCACCAAGAG GATGGTGGGG TTGTGACGGT GCATCCAGAT
    GGCAACTCCT TCGACATGCT GAGAAACCTC ATCGTGCCCA AGCGACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52629
    Clone ID Related Accession (Same CDS sequence) XM_006522853.4 , XM_006522853.3
    Accession Version XM_006522853.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522853.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCCTAG CACAGCCCCT CTCACCTCCC CAGTTCACTC TAGTTCTGCA GCCCAGGTCT 
    CTCCATCTCC AGAGCACACC TTCAATCTCC TGGCTCACCT TTCCCCCAGC CCCTCTTGGA
    AAAGCCAGCC TCCTGAGACC AGACAGTGAC CTCTCTTCCA ATGCCATCTT CAGTCCTGCC
    TGCCCTTTCC ATTCCTGTAG CCTCTCTTTA CCCGAGGCAC CCTCTCCATG GTCCTGGCGC
    CCCCAGATGC TACCCAGCCT GCTGCATCCT TCCACCTCTA GCGCATCCCT CTCCTGCATC
    CTTCCTTGCC CCGCACACCC GCTCCGCCCT CAGCCCCCCT CCTGCAGCGC ACGCGCACTC
    CACACTCCCG AAGCCTCGCT TCCGGCCGGG GCGGGGCCTG GCGCGAGCCG GGCGCGGGCG
    CCGGCCATGC TCATCACGCT GTGCTACCTG TACCTGTGGG CGCGCTGGGG GCGCCGGCCG
    GCCGCGCTGG TGCTGTCCAC GGTGCGGCGG CTGCGTGCTT CGCGCTGCTC CTTCACCTTC
    TGCCGCGCGG CCGCGCAGCC CAGGGACGCG CGCGTGTGCC TGAGCCAGGG CGGCCGCGTC
    TTCTGCGTCG GCGAGAGCCA GTCTGTTGAT GACTTGAACA AATGGGCCCT GTTTCTCGTG
    TCTCCCTTCA TACTTGAGGC AGAACATATA GCATTTGTGA CAGAGAGCAT TTGGATACAA
    GGTACCAGTT TACAAGAGCC ATCATCTTCA GAAACCGTTG TAAAATGGTC TGACTGCTGT
    CTGCCGTTAG CCTGCCGACC CGGGGACCCC TACCAGTTAA TTGCTAAGGC AAGCGTGGAC
    AACTTCAGCA AGCTTGGGGT GGCCTTCATG GAGGACAGGC TCCAGATGGA CAACGGCCTG
    ATAGCCCAGA AGATCGTTTC AGTCCACTTA AAGGACCCTG CTCTGAAGGA ACTCGGCAAA
    GCCTCCGACA AGCAAGTGCA GGGCCCGCCC CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG
    CCTGCACAAG GTGTCATGGA GCATGCTGGC CAAGGTGATT GCAAGGCCGC CGGTGAAGGC
    CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT GAAAGTGAGC CTGCTGCAGA TGGGGACCCA
    GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC CACCTGTCCA TCTGTCACGA GTGTCTGGAG
    CTCGAGAACA GTACCATTGA CTCGGTGAGG TCTGCGTCTG CGGAGAACAT TCCAGACCTT
    CCCTGTGATC ACAGCGGCGT GGAGGGTGCT GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG
    AAGAGAGTCA ACATTTCGGG AAAGGCGCCC AATATCTTGC TGTATGTGGG CTCTGGTTCC
    GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG CGATCTGTCC TGACTGACTG TGTGGACACG
    GACAGCTACA CTCTCTACCA CCTGCTGGAA GACAGCGCTC TGAGGGACCC GTGGTCTGAC
    AACTGCCTCC TGCTAGTTAT TGCCAGCAGG GACCCCATCC CCAAAGACAT ACAGCACAAG
    TTCATGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGGCC TGTCTTCCCC CTTCACGTTG
    GGTGGCTTTC GGGTGACCAG GAGAGATGTG CTACGGAACA CAGTCCAGAA CTTGGTTTTC
    TCTAAGGCTG ATGGGACTGA GGTACGGCTC AGTGTCCTGA GCAGCGGCTA CGTTTATGAA
    GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC CACCTGGAGA ATGAGGACAA GGATAAAATG
    ATTGTGCACG TGCCTTTTGG AACCCTCGGA GGGGAAGCCG TTCTCTGCCA GGTGCACCTG
    GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG ACTGCAGACG ACTTTAACGT GCTCAAATCC
    AGCAATGTGA GAAGACACGA GGTCCTTAAG GAGATCCTGA CTGCCCTCGG TCTGAGCTGT
    GACGCACCAC AGGTTCCTGC CTTAACGCCC CTGTACTTAC TGCTGGCCGC TGAGGAAACC
    CAGGATCCGT TCATGCAGTG GCTCGGGAGG CACACAGATC CTGAAGGAAT CATAAAATCC
    AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC TACACATCTG AAGCGGAGAT CACCCCGTCT
    TCCATGCCTG TGGTCACTGA CCCAGAAGCC TTTTCATCAG AGCACTTCAG CCTAGAGACC
    TATCGACAGA ATCTGCAGAC CACGCGCCTT GGGAAAGTGA TTCTGTTTGC AGAAGTGACC
    TCCACCACCA TGAGTCTCCT GGATGGGCTC CCGGGACTTC CCTCACAGTT GGCTTCCATG
    AGTCCCATCT ATCAGAGTTC ACTGGACAAT CTTTGGCTCC CTGCAGTGTT GTGGGTAATT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522916.1
    CDS281..2683
    Translation

    Target ORF information:

    RefSeq Version XM_006522853.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522853.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCCTAG CACAGCCCCT CTCACCTCCC CAGTTCACTC TAGTTCTGCA GCCCAGGTCT 
    CTCCATCTCC AGAGCACACC TTCAATCTCC TGGCTCACCT TTCCCCCAGC CCCTCTTGGA
    AAAGCCAGCC TCCTGAGACC AGACAGTGAC CTCTCTTCCA ATGCCATCTT CAGTCCTGCC
    TGCCCTTTCC ATTCCTGTAG CCTCTCTTTA CCCGAGGCAC CCTCTCCATG GTCCTGGCGC
    CCCCAGATGC TACCCAGCCT GCTGCATCCT TCCACCTCTA GCGCATCCCT CTCCTGCATC
    CTTCCTTGCC CCGCACACCC GCTCCGCCCT CAGCCCCCCT CCTGCAGCGC ACGCGCACTC
    CACACTCCCG AAGCCTCGCT TCCGGCCGGG GCGGGGCCTG GCGCGAGCCG GGCGCGGGCG
    CCGGCCATGC TCATCACGCT GTGCTACCTG TACCTGTGGG CGCGCTGGGG GCGCCGGCCG
    GCCGCGCTGG TGCTGTCCAC GGTGCGGCGG CTGCGTGCTT CGCGCTGCTC CTTCACCTTC
    TGCCGCGCGG CCGCGCAGCC CAGGGACGCG CGCGTGTGCC TGAGCCAGGG CGGCCGCGTC
    TTCTGCGTCG GCGAGAGCCA GTCTGTTGAT GACTTGAACA AATGGGCCCT GTTTCTCGTG
    TCTCCCTTCA TACTTGAGGC AGAACATATA GCATTTGTGA CAGAGAGCAT TTGGATACAA
    GGTACCAGTT TACAAGAGCC ATCATCTTCA GAAACCGTTG TAAAATGGTC TGACTGCTGT
    CTGCCGTTAG CCTGCCGACC CGGGGACCCC TACCAGTTAA TTGCTAAGGC AAGCGTGGAC
    AACTTCAGCA AGCTTGGGGT GGCCTTCATG GAGGACAGGC TCCAGATGGA CAACGGCCTG
    ATAGCCCAGA AGATCGTTTC AGTCCACTTA AAGGACCCTG CTCTGAAGGA ACTCGGCAAA
    GCCTCCGACA AGCAAGTGCA GGGCCCGCCC CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG
    CCTGCACAAG GTGTCATGGA GCATGCTGGC CAAGGTGATT GCAAGGCCGC CGGTGAAGGC
    CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT GAAAGTGAGC CTGCTGCAGA TGGGGACCCA
    GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC CACCTGTCCA TCTGTCACGA GTGTCTGGAG
    CTCGAGAACA GTACCATTGA CTCGGTGAGG TCTGCGTCTG CGGAGAACAT TCCAGACCTT
    CCCTGTGATC ACAGCGGCGT GGAGGGTGCT GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG
    AAGAGAGTCA ACATTTCGGG AAAGGCGCCC AATATCTTGC TGTATGTGGG CTCTGGTTCC
    GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG CGATCTGTCC TGACTGACTG TGTGGACACG
    GACAGCTACA CTCTCTACCA CCTGCTGGAA GACAGCGCTC TGAGGGACCC GTGGTCTGAC
    AACTGCCTCC TGCTAGTTAT TGCCAGCAGG GACCCCATCC CCAAAGACAT ACAGCACAAG
    TTCATGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGGCC TGTCTTCCCC CTTCACGTTG
    GGTGGCTTTC GGGTGACCAG GAGAGATGTG CTACGGAACA CAGTCCAGAA CTTGGTTTTC
    TCTAAGGCTG ATGGGACTGA GGTACGGCTC AGTGTCCTGA GCAGCGGCTA CGTTTATGAA
    GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC CACCTGGAGA ATGAGGACAA GGATAAAATG
    ATTGTGCACG TGCCTTTTGG AACCCTCGGA GGGGAAGCCG TTCTCTGCCA GGTGCACCTG
    GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG ACTGCAGACG ACTTTAACGT GCTCAAATCC
    AGCAATGTGA GAAGACACGA GGTCCTTAAG GAGATCCTGA CTGCCCTCGG TCTGAGCTGT
    GACGCACCAC AGGTTCCTGC CTTAACGCCC CTGTACTTAC TGCTGGCCGC TGAGGAAACC
    CAGGATCCGT TCATGCAGTG GCTCGGGAGG CACACAGATC CTGAAGGAAT CATAAAATCC
    AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC TACACATCTG AAGCGGAGAT CACCCCGTCT
    TCCATGCCTG TGGTCACTGA CCCAGAAGCC TTTTCATCAG AGCACTTCAG CCTAGAGACC
    TATCGACAGA ATCTGCAGAC CACGCGCCTT GGGAAAGTGA TTCTGTTTGC AGAAGTGACC
    TCCACCACCA TGAGTCTCCT GGATGGGCTC CCGGGACTTC CCTCACAGTT GGCTTCCATG
    AGTCCCATCT ATCAGAGTTC ACTGGACAAT CTTTGGCTCC CTGCAGTGTT GTGGGTAATT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52630
    Clone ID Related Accession (Same CDS sequence) XM_006522854.4 , XM_006522854.3
    Accession Version XM_006522854.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522854.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCTAG CACAGCCCCT CTCACCTCCC CAGTTCACTC TAGTTCTGCA GCCCAGGTCT 
    CTCCATCTCC AGAGCACACC TTCAATCTCC TGGCTCACCT TTCCCCCAGC CCCTCTTGGA
    AAAGCCAGCC TCCTGAGACC AGACAGTGAC CTCTCTTCCA ATGCCATCTT CAGTCCTGCC
    TGCCCTTTCC ATTCCTGTAG CCTCTCTTTA CCCGAGGCAC CCTCTCCATG GTCCTGGCGC
    CCCCAGATGC TACCCAGCCT GCTGCATCCT TCCACCTCTA GCGCATCCCT CTCCTGCATC
    CTTCCTTGCC CCGCACACCC GCTCCGCCCT CAGCCCCCCT CCTGCAGCGC ACGCGCACTC
    CACACTCCCG AAGCCTCGCT TCCGGCCGGG GCGGGGCCTG GCGCGAGCCG GGCGCGGGCG
    CCGGCCATGC TCATCACGCT GTGCTACCTG TACCTGTGGG CGCGCTGGGG GCGCCGGCCG
    GCCGCGCTGG TGCTGTCCAC GGTGCGGCGG CTGCGTGCTT CGCGCTGCTC CTTCACCTTC
    TGCCGCGCGG CCGCGCAGCC CAGGGACGCG CGCGTGTGCC TGAGCCAGGG CGGCCGCGTC
    TTCTGCGTCG GCGAGAGCCA GTCTGTTGAT GACTTGAACA AATGGGCCCT GTTTCTCGTG
    TCTCCCTTCA TACTTGAGGC AGAACATATA GCATTTGTGA CAGAGAGCAT TTGGATACAA
    GGTACCAGTT TACAAGAGCC ATCATCTTCA GAAACCGTTG TAAAATGGTC TGACTGCTGT
    CTGCCGTTAG CCTGCCGACC CGGGGACCCC TACCAGTTAA TTGCTAAGGC AAGCGTGGAC
    AACTTCAGCA AGCTTGGGGT GGCCTTCATG GAGGACAGGC TCCAGATGGA CAACGGCCTG
    ATAGCCCAGA AGATCGTTTC AGTCCACTTA AAGGACCCTG CTCTGAAGGA ACTCGGCAAA
    GCCTCCGACA AGCAAGTGCA GGGCCCGCCC CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG
    CCTGCACAAG GTGTCATGGA GCATGCTGGC CAAGGTGATT GCAAGGCCGC CGGTGAAGGC
    CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT GAAAGTGAGC CTGCTGCAGA TGGGGACCCA
    GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC CACCTGTCCA TCTGTCACGA GTGTCTGGAG
    CTCGAGAACA GTACCATTGA CTCGGTGAGG TCTGCGTCTG CGGAGAACAT TCCAGACCTT
    CCCTGTGATC ACAGCGGCGT GGAGGGTGCT GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG
    AAGAGAGTCA ACATTTCGGG AAAGGCGCCC AATATCTTGC TGTATGTGGG CTCTGGTTCC
    GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG CGATCTGTCC TGACTGACTG TGTGGACACG
    GACAGCTACA CTCTCTACCA CCTGCTGGAA GACAGCGCTC TGAGGGACCC GTGGTCTGAC
    AACTGCCTCC TGCTAGTTAT TGCCAGCAGG GACCCCATCC CCAAAGACAT ACAGCACAAG
    TTCATGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGGCC TGTCTTCCCC CTTCACGTTG
    GGTGGCTTTC GGGTGACCAG GAGAGATGTG CTACGGAACA CAGTCCAGAA CTTGGTTTTC
    TCTAAGGCTG ATGGGACTGA GGTACGGCTC AGTGTCCTGA GCAGCGGCTA CGTTTATGAA
    GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC CACCTGGAGA ATGAGGACAA GGATAAAATG
    ATTGTGCACG TGCCTTTTGG AACCCTCGGA GGGGAAGCCG TTCTCTGCCA GGTGCACCTG
    GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG ACTGCAGACG ACTTTAACGT GCTCAAATCC
    AGCAATGTGA GAAGACACGA GGTCCTTAAG GAGATCCTGA CTGCCCTCGG TCTGAGCTGT
    GACGCACCAC AGGTTCCTGC CTTAACGCCC CTGTACTTAC TGCTGGCCGC TGAGGAAACC
    CAGGATCCGT TCATGCAGTG GCTCGGGAGG CACACAGATC CTGAAGGAAT CATAAAATCC
    AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC TACACATCTG AAGCGGAGAT CACCCCGTCT
    TCCATGCCTG TGGTCACTGA CCCAGAAGCC TTTTCATCAG AGCACTTCAG CCTAGAGACC
    TATCGACAGA ATCTGCAGAC CACGCGCCTT GGGAAAGTGA TTCTGTTTGC AGAAGTGACC
    TCCACCACCA TGAGTCTCCT GGATGGAAGG AACGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522917.1
    CDS279..2597
    Translation

    Target ORF information:

    RefSeq Version XM_006522854.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522854.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCTAG CACAGCCCCT CTCACCTCCC CAGTTCACTC TAGTTCTGCA GCCCAGGTCT 
    CTCCATCTCC AGAGCACACC TTCAATCTCC TGGCTCACCT TTCCCCCAGC CCCTCTTGGA
    AAAGCCAGCC TCCTGAGACC AGACAGTGAC CTCTCTTCCA ATGCCATCTT CAGTCCTGCC
    TGCCCTTTCC ATTCCTGTAG CCTCTCTTTA CCCGAGGCAC CCTCTCCATG GTCCTGGCGC
    CCCCAGATGC TACCCAGCCT GCTGCATCCT TCCACCTCTA GCGCATCCCT CTCCTGCATC
    CTTCCTTGCC CCGCACACCC GCTCCGCCCT CAGCCCCCCT CCTGCAGCGC ACGCGCACTC
    CACACTCCCG AAGCCTCGCT TCCGGCCGGG GCGGGGCCTG GCGCGAGCCG GGCGCGGGCG
    CCGGCCATGC TCATCACGCT GTGCTACCTG TACCTGTGGG CGCGCTGGGG GCGCCGGCCG
    GCCGCGCTGG TGCTGTCCAC GGTGCGGCGG CTGCGTGCTT CGCGCTGCTC CTTCACCTTC
    TGCCGCGCGG CCGCGCAGCC CAGGGACGCG CGCGTGTGCC TGAGCCAGGG CGGCCGCGTC
    TTCTGCGTCG GCGAGAGCCA GTCTGTTGAT GACTTGAACA AATGGGCCCT GTTTCTCGTG
    TCTCCCTTCA TACTTGAGGC AGAACATATA GCATTTGTGA CAGAGAGCAT TTGGATACAA
    GGTACCAGTT TACAAGAGCC ATCATCTTCA GAAACCGTTG TAAAATGGTC TGACTGCTGT
    CTGCCGTTAG CCTGCCGACC CGGGGACCCC TACCAGTTAA TTGCTAAGGC AAGCGTGGAC
    AACTTCAGCA AGCTTGGGGT GGCCTTCATG GAGGACAGGC TCCAGATGGA CAACGGCCTG
    ATAGCCCAGA AGATCGTTTC AGTCCACTTA AAGGACCCTG CTCTGAAGGA ACTCGGCAAA
    GCCTCCGACA AGCAAGTGCA GGGCCCGCCC CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG
    CCTGCACAAG GTGTCATGGA GCATGCTGGC CAAGGTGATT GCAAGGCCGC CGGTGAAGGC
    CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT GAAAGTGAGC CTGCTGCAGA TGGGGACCCA
    GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC CACCTGTCCA TCTGTCACGA GTGTCTGGAG
    CTCGAGAACA GTACCATTGA CTCGGTGAGG TCTGCGTCTG CGGAGAACAT TCCAGACCTT
    CCCTGTGATC ACAGCGGCGT GGAGGGTGCT GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG
    AAGAGAGTCA ACATTTCGGG AAAGGCGCCC AATATCTTGC TGTATGTGGG CTCTGGTTCC
    GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG CGATCTGTCC TGACTGACTG TGTGGACACG
    GACAGCTACA CTCTCTACCA CCTGCTGGAA GACAGCGCTC TGAGGGACCC GTGGTCTGAC
    AACTGCCTCC TGCTAGTTAT TGCCAGCAGG GACCCCATCC CCAAAGACAT ACAGCACAAG
    TTCATGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGGCC TGTCTTCCCC CTTCACGTTG
    GGTGGCTTTC GGGTGACCAG GAGAGATGTG CTACGGAACA CAGTCCAGAA CTTGGTTTTC
    TCTAAGGCTG ATGGGACTGA GGTACGGCTC AGTGTCCTGA GCAGCGGCTA CGTTTATGAA
    GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC CACCTGGAGA ATGAGGACAA GGATAAAATG
    ATTGTGCACG TGCCTTTTGG AACCCTCGGA GGGGAAGCCG TTCTCTGCCA GGTGCACCTG
    GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG ACTGCAGACG ACTTTAACGT GCTCAAATCC
    AGCAATGTGA GAAGACACGA GGTCCTTAAG GAGATCCTGA CTGCCCTCGG TCTGAGCTGT
    GACGCACCAC AGGTTCCTGC CTTAACGCCC CTGTACTTAC TGCTGGCCGC TGAGGAAACC
    CAGGATCCGT TCATGCAGTG GCTCGGGAGG CACACAGATC CTGAAGGAAT CATAAAATCC
    AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC TACACATCTG AAGCGGAGAT CACCCCGTCT
    TCCATGCCTG TGGTCACTGA CCCAGAAGCC TTTTCATCAG AGCACTTCAG CCTAGAGACC
    TATCGACAGA ATCTGCAGAC CACGCGCCTT GGGAAAGTGA TTCTGTTTGC AGAAGTGACC
    TCCACCACCA TGAGTCTCCT GGATGGAAGG AACGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu86856
    Clone ID Related Accession (Same CDS sequence) XM_030248921.1
    Accession Version XM_030248921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTGTCTGG CACGCGGTGC TGCGCGAATG GCAAGCCTGG CTTTGCAGGG GACAGGGAGC 
    TGGGGATCCA GATTGCAGGG GCAGATGGCA GACTGGCTGC GCGTCCCCGA CGCAAGGGCA
    GCTTTGCGCA GTGCGCATCC CCGCGCGCTG CTCGCCGCGG CCGCCAGGGG GCGCAGCGGG
    ATCTCAGTGC GATCACCCTG CGGAGCGGGC AGCCGCGCTC AGGGCTGCGA GTCTGTTGAT
    GACTTGAACA AATGGGCCCT GTTTCTCGTG TCTCCCTTCA TACTTGAGGC AGAACATATA
    GCATTTGTGA CAGAGAGCAT TTGGATACAA GGTACCAGTT TACAAGAGCC ATCATCTTCA
    GAAACCGTTG TAAAATGGTC TGACTGCTGT CTGCCGTTAG CCTGCCGACC CGGGGACCCC
    TACCAGTTAA TTGCTAAGGC AAGCGTGGAC AACTTCAGCA AGCTTGGGGT GGCCTTCATG
    GAGGACAGGC TCCAGATGGA CAACGGCCTG ATAGCCCAGA AGATCGTTTC AGTCCACTTA
    AAGGACCCTG CTCTGAAGGA ACTCGGCAAA GCCTCCGACA AGCAAGTGCA GGGCCCGCCC
    CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG CCTGCACAAG GTGTCATGGA GCATGCTGGC
    CAAGGTGATT GCAAGGCCGC CGGTGAAGGC CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT
    GAAAGTGAGC CTGCTGCAGA TGGGGACCCA GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC
    CACCTGTCCA TCTGTCACGA GTGTCTGGAG CTCGAGAACA GTACCATTGA CTCGGTGAGG
    TCTGCGTCTG CGGAGAACAT TCCAGACCTT CCCTGTGATC ACAGCGGCGT GGAGGGTGCT
    GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG AAGAGAGTCA ACATTTCGGG AAAGGCGCCC
    AATATCTTGC TGTATGTGGG CTCTGGTTCC GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG
    CGATCTGTCC TGACTGACTG TGTGGACACG GACAGCTACA CTCTCTACCA CCTGCTGGAA
    GACAGCGCTC TGAGGGACCC GTGGTCTGAC AACTGCCTCC TGCTAGTTAT TGCCAGCAGG
    GACCCCATCC CCAAAGACAT ACAGCACAAG TTCATGGCCT ATCTTTCTCA GGGAGGGAAG
    GTGCTGGGCC TGTCTTCCCC CTTCACGTTG GGTGGCTTTC GGGTGACCAG GAGAGATGTG
    CTACGGAACA CAGTCCAGAA CTTGGTTTTC TCTAAGGCTG ATGGGACTGA GGTACGGCTC
    AGTGTCCTGA GCAGCGGCTA CGTTTATGAA GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC
    CACCTGGAGA ATGAGGACAA GGATAAAATG ATTGTGCACG TGCCTTTTGG AACCCTCGGA
    GGGGAAGCCG TTCTCTGCCA GGTGCACCTG GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG
    ACTGCAGACG ACTTTAACGT GCTCAAATCC AGCAATGTGA GAAGACACGA GGTCCTTAAG
    GAGATCCTGA CTGCCCTCGG TCTGAGCTGT GACGCACCAC AGGTTCCTGC CTTAACGCCC
    CTGTACTTAC TGCTGGCCGC TGAGGAAACC CAGGATCCGT TCATGCAGTG GCTCGGGAGG
    CACACAGATC CTGAAGGAAT CATAAAATCC AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC
    TACACATCTG AAGCGGAGAT CACCCCGTCT TCCATGCCTG TGGTCACTGA CCCAGAAGCC
    TTTTCATCAG AGCACTTCAG CCTAGAGACC TATCGACAGA ATCTGCAGAC CACGCGCCTT
    GGGAAAGTGA TTCTGTTTGC AGAAGTGACC TCCACCACCA TGAGTCTCCT GGATGGAAGG
    AACGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030104781.1
    CDS1492..3420
    Translation

    Target ORF information:

    RefSeq Version XM_030248921.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030248921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTGTCTGG CACGCGGTGC TGCGCGAATG GCAAGCCTGG CTTTGCAGGG GACAGGGAGC 
    TGGGGATCCA GATTGCAGGG GCAGATGGCA GACTGGCTGC GCGTCCCCGA CGCAAGGGCA
    GCTTTGCGCA GTGCGCATCC CCGCGCGCTG CTCGCCGCGG CCGCCAGGGG GCGCAGCGGG
    ATCTCAGTGC GATCACCCTG CGGAGCGGGC AGCCGCGCTC AGGGCTGCGA GTCTGTTGAT
    GACTTGAACA AATGGGCCCT GTTTCTCGTG TCTCCCTTCA TACTTGAGGC AGAACATATA
    GCATTTGTGA CAGAGAGCAT TTGGATACAA GGTACCAGTT TACAAGAGCC ATCATCTTCA
    GAAACCGTTG TAAAATGGTC TGACTGCTGT CTGCCGTTAG CCTGCCGACC CGGGGACCCC
    TACCAGTTAA TTGCTAAGGC AAGCGTGGAC AACTTCAGCA AGCTTGGGGT GGCCTTCATG
    GAGGACAGGC TCCAGATGGA CAACGGCCTG ATAGCCCAGA AGATCGTTTC AGTCCACTTA
    AAGGACCCTG CTCTGAAGGA ACTCGGCAAA GCCTCCGACA AGCAAGTGCA GGGCCCGCCC
    CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG CCTGCACAAG GTGTCATGGA GCATGCTGGC
    CAAGGTGATT GCAAGGCCGC CGGTGAAGGC CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT
    GAAAGTGAGC CTGCTGCAGA TGGGGACCCA GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC
    CACCTGTCCA TCTGTCACGA GTGTCTGGAG CTCGAGAACA GTACCATTGA CTCGGTGAGG
    TCTGCGTCTG CGGAGAACAT TCCAGACCTT CCCTGTGATC ACAGCGGCGT GGAGGGTGCT
    GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG AAGAGAGTCA ACATTTCGGG AAAGGCGCCC
    AATATCTTGC TGTATGTGGG CTCTGGTTCC GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG
    CGATCTGTCC TGACTGACTG TGTGGACACG GACAGCTACA CTCTCTACCA CCTGCTGGAA
    GACAGCGCTC TGAGGGACCC GTGGTCTGAC AACTGCCTCC TGCTAGTTAT TGCCAGCAGG
    GACCCCATCC CCAAAGACAT ACAGCACAAG TTCATGGCCT ATCTTTCTCA GGGAGGGAAG
    GTGCTGGGCC TGTCTTCCCC CTTCACGTTG GGTGGCTTTC GGGTGACCAG GAGAGATGTG
    CTACGGAACA CAGTCCAGAA CTTGGTTTTC TCTAAGGCTG ATGGGACTGA GGTACGGCTC
    AGTGTCCTGA GCAGCGGCTA CGTTTATGAA GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC
    CACCTGGAGA ATGAGGACAA GGATAAAATG ATTGTGCACG TGCCTTTTGG AACCCTCGGA
    GGGGAAGCCG TTCTCTGCCA GGTGCACCTG GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG
    ACTGCAGACG ACTTTAACGT GCTCAAATCC AGCAATGTGA GAAGACACGA GGTCCTTAAG
    GAGATCCTGA CTGCCCTCGG TCTGAGCTGT GACGCACCAC AGGTTCCTGC CTTAACGCCC
    CTGTACTTAC TGCTGGCCGC TGAGGAAACC CAGGATCCGT TCATGCAGTG GCTCGGGAGG
    CACACAGATC CTGAAGGAAT CATAAAATCC AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC
    TACACATCTG AAGCGGAGAT CACCCCGTCT TCCATGCCTG TGGTCACTGA CCCAGAAGCC
    TTTTCATCAG AGCACTTCAG CCTAGAGACC TATCGACAGA ATCTGCAGAC CACGCGCCTT
    GGGAAAGTGA TTCTGTTTGC AGAAGTGACC TCCACCACCA TGAGTCTCCT GGATGGAAGG
    AACGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52626
    Clone ID Related Accession (Same CDS sequence) XM_006522850.3
    Accession Version XM_006522850.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522850.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCCCTAG CACAGCCCCT CTCACCTCCC CAGTTCACTC TAGTTCTGCA GCCCAGGTCT 
    CTCCATCTCC AGAGCACACC TTCAATCTCC TGGCTCACCT TTCCCCCAGC CCCTCTTGGA
    AAAGCCAGCC TCCTGAGACC AGACAGTGAC CTCTCTTCCA ATGCCATCTT CAGTCCTGCC
    TGCCCTTTCC ATTCCTGTAG CCTCTCTTTA CCCGAGGCAC CCTCTCCATG GTCCTGGCGC
    CCCCAGATGC TACCCAGCCT GCTGCATCCT TCCACCTCTA GCGCATCCCT CTCCTGCATC
    CTTCCTTGCC CCGCACACCC GCTCCGCCCT CAGCCCCCCT CCTGCAGCGC ACGCGCACTC
    CACACTCCCG AAGCCTCGCT TCCGGCCGGG GCGGGGCCTG GCGCGAGCCG GGCGCGGGCG
    CCGGCCATGC TCATCACGCT GTGCTACCTG TACCTGTGGG CGCGCTGGGG GCGCCGGCCG
    GCCGCGCTGG TGCTGTCCAC GGTGCGGCGG CTGCGTGCTT CGCGCTGCTC CTTCACCTTC
    TGCCGCGCGG CCGCGCAGCC CAGGGACGCG CGCGTGTGCC TGAGCCAGGG CGGCCGCGTC
    TTCTGCGTCG GCGAGAGCCA GTCTGTTGAT GACTTGAACA AATGGGCCCT GTTTCTCGTG
    TCTCCCTTCA TACTTGAGGC AGAACATATA GCATTTGTGA CAGAGAGCAT TTGGATACAA
    GGTACCAGTT TACAAGAGCC ATCATCTTCA GAAACCGTTG TAAAATGGTC TGACTGCTGT
    CTGCCGTTAG CCTGCCGACC CGGGGACCCC TACCAGTTAA TTGCTAAGGC AAGCGTGGAC
    AACTTCAGCA AGCTTGGGGT GGCCTTCATG GAGGACAGGC TCCAGATGGA CAACGGCCTG
    ATAGCCCAGA AGATCGTTTC AGTCCACTTA AAGGACCCTG CTCTGAAGGA ACTCGGCAAA
    GCCTCCGACA AGCAAGTGCA GGGCCCGCCC CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG
    CCTGCACAAG GTGTCATGGA GCATGCTGGC CAAGGTGATT GCAAGGCCGC CGGTGAAGGC
    CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT GAAAGTGAGC CTGCTGCAGA TGGGGACCCA
    GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC CACCTGTCCA TCTGTCACGA GTGTCTGGAG
    CTCGAGAACA GTACCATTGA CTCGGTGAGG TCTGCGTCTG CGGAGAACAT TCCAGACCTT
    CCCTGTGATC ACAGCGGCGT GGAGGGTGCT GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG
    AAGAGAGTCA ACATTTCGGG AAAGGCGCCC AATATCTTGC TGTATGTGGG CTCTGGTTCC
    GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG CGATCTGTCC TGACTGACTG TGTGGACACG
    GACAGCTACA CTCTCTACCA CCTGCTGGAA GACAGCGCTC TGAGGGACCC GTGGTCTGAC
    AACTGCCTCC TGCTAGTTAT TGCCAGCAGG GACCCCATCC CCAAAGACAT ACAGCACAAG
    TTCATGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGGCC TGTCTTCCCC CTTCACGTTG
    GGTGGCTTTC GGGTGACCAG GAGAGATGTG CTACGGAACA CAGTCCAGAA CTTGGTTTTC
    TCTAAGGCTG ATGGGACTGA GGTACGGCTC AGTGTCCTGA GCAGCGGCTA CGTTTATGAA
    GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC CACCTGGAGA ATGAGGACAA GGATAAAATG
    ATTGTGCACG TGCCTTTTGG AACCCTCGGA GGGGAAGCCG TTCTCTGCCA GGTGCACCTG
    GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG ACTGCAGACG ACTTTAACGT GCTCAAATCC
    AGCAATGTGA GAAGACACGA GGTCCTTAAG GAGATCCTGA CTGCCCTCGG TCTGAGCTGT
    GACGCACCAC AGGTTCCTGC CTTAACGCCC CTGTACTTAC TGCTGGCCGC TGAGGAAACC
    CAGGATCCGT TCATGCAGTG GCTCGGGAGG CACACAGATC CTGAAGGAAT CATAAAATCC
    AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC TACACATCTG AAGCGGAGAT CACCCCGTCT
    TCCATGCCTG TGGTCACTGA CCCAGAAGCC TTTTCATCAG AGCACTTCAG CCTAGAGACC
    TATCGACAGA ATCTGCAGAC CACGCGCCTT GGGAAAGTGA TTCTGTTTGC AGAAGTGACC
    TCCACCACCA TGAGTCTCCT GGATGGGTTG ATGTTTGAGA TGCCGCAGGA AATGGGCTTA
    ATTGCCATTG CAGTTCGGCA GACCCAGGGC AAAGGAAGGG GCCCGAATGC CTGGCTGAGC
    CCCGTGGGAT GTGCTCTTTC CACTCTGCTG GTCTTCATCC CCCTGAGGTC ACAACTGGGA
    CAGAGGATTC CATTTGTCCA GCATTTGATG TCCTTGGCTG TGGTGGAGGC AGTGCGGTCT
    ATCCCAGGGT ATGAGGATAT CAACTTGCGA GTGAAGTGGC CCAACGATAT TTATTACAGC
    GATCTCATGA AGATCGGTGG GGTTCTGGTT AATTCAACAC TTATGGGAGA AACATTTTAT
    ATCCTCATCG GCTGTGGATT CAATGTGACG AACAGTAATC CTACCATTTG TATCAACGAC
    CTCATCGAAG AACACAATAA GCAGCACGGG GCGGGCCTGA AGCCCCTGCG AGCGGACTGT
    CTCATAGCCA GAGCCGTGAC CGTGCTGGAG AAACTGATCG ACAGGTTTCA GGACCAAGGG
    CCCGACGGCG TTCTTCCCCT CTATTATAAA TACTGGGTGC ACGGTGGTCA GCAAGTCCGC
    CTGGGCAGCA CGGAGGGGCC ACAGGCATCT ATTGTGGGCC TTGATGACTC CGGGTTCCTG
    CAGGTCCACC AAGAGGATGG TGGGGTTGTG ACGGTGCATC CAGATGGCAA CTCCTTCGAC
    ATGCTGAGAA ACCTCATCGT GCCCAAGCGA CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522913.1
    CDS297..3332
    Translation

    Target ORF information:

    RefSeq Version XM_006522850.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522850.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCCCTAG CACAGCCCCT CTCACCTCCC CAGTTCACTC TAGTTCTGCA GCCCAGGTCT 
    CTCCATCTCC AGAGCACACC TTCAATCTCC TGGCTCACCT TTCCCCCAGC CCCTCTTGGA
    AAAGCCAGCC TCCTGAGACC AGACAGTGAC CTCTCTTCCA ATGCCATCTT CAGTCCTGCC
    TGCCCTTTCC ATTCCTGTAG CCTCTCTTTA CCCGAGGCAC CCTCTCCATG GTCCTGGCGC
    CCCCAGATGC TACCCAGCCT GCTGCATCCT TCCACCTCTA GCGCATCCCT CTCCTGCATC
    CTTCCTTGCC CCGCACACCC GCTCCGCCCT CAGCCCCCCT CCTGCAGCGC ACGCGCACTC
    CACACTCCCG AAGCCTCGCT TCCGGCCGGG GCGGGGCCTG GCGCGAGCCG GGCGCGGGCG
    CCGGCCATGC TCATCACGCT GTGCTACCTG TACCTGTGGG CGCGCTGGGG GCGCCGGCCG
    GCCGCGCTGG TGCTGTCCAC GGTGCGGCGG CTGCGTGCTT CGCGCTGCTC CTTCACCTTC
    TGCCGCGCGG CCGCGCAGCC CAGGGACGCG CGCGTGTGCC TGAGCCAGGG CGGCCGCGTC
    TTCTGCGTCG GCGAGAGCCA GTCTGTTGAT GACTTGAACA AATGGGCCCT GTTTCTCGTG
    TCTCCCTTCA TACTTGAGGC AGAACATATA GCATTTGTGA CAGAGAGCAT TTGGATACAA
    GGTACCAGTT TACAAGAGCC ATCATCTTCA GAAACCGTTG TAAAATGGTC TGACTGCTGT
    CTGCCGTTAG CCTGCCGACC CGGGGACCCC TACCAGTTAA TTGCTAAGGC AAGCGTGGAC
    AACTTCAGCA AGCTTGGGGT GGCCTTCATG GAGGACAGGC TCCAGATGGA CAACGGCCTG
    ATAGCCCAGA AGATCGTTTC AGTCCACTTA AAGGACCCTG CTCTGAAGGA ACTCGGCAAA
    GCCTCCGACA AGCAAGTGCA GGGCCCGCCC CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG
    CCTGCACAAG GTGTCATGGA GCATGCTGGC CAAGGTGATT GCAAGGCCGC CGGTGAAGGC
    CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT GAAAGTGAGC CTGCTGCAGA TGGGGACCCA
    GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC CACCTGTCCA TCTGTCACGA GTGTCTGGAG
    CTCGAGAACA GTACCATTGA CTCGGTGAGG TCTGCGTCTG CGGAGAACAT TCCAGACCTT
    CCCTGTGATC ACAGCGGCGT GGAGGGTGCT GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG
    AAGAGAGTCA ACATTTCGGG AAAGGCGCCC AATATCTTGC TGTATGTGGG CTCTGGTTCC
    GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG CGATCTGTCC TGACTGACTG TGTGGACACG
    GACAGCTACA CTCTCTACCA CCTGCTGGAA GACAGCGCTC TGAGGGACCC GTGGTCTGAC
    AACTGCCTCC TGCTAGTTAT TGCCAGCAGG GACCCCATCC CCAAAGACAT ACAGCACAAG
    TTCATGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGGCC TGTCTTCCCC CTTCACGTTG
    GGTGGCTTTC GGGTGACCAG GAGAGATGTG CTACGGAACA CAGTCCAGAA CTTGGTTTTC
    TCTAAGGCTG ATGGGACTGA GGTACGGCTC AGTGTCCTGA GCAGCGGCTA CGTTTATGAA
    GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC CACCTGGAGA ATGAGGACAA GGATAAAATG
    ATTGTGCACG TGCCTTTTGG AACCCTCGGA GGGGAAGCCG TTCTCTGCCA GGTGCACCTG
    GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG ACTGCAGACG ACTTTAACGT GCTCAAATCC
    AGCAATGTGA GAAGACACGA GGTCCTTAAG GAGATCCTGA CTGCCCTCGG TCTGAGCTGT
    GACGCACCAC AGGTTCCTGC CTTAACGCCC CTGTACTTAC TGCTGGCCGC TGAGGAAACC
    CAGGATCCGT TCATGCAGTG GCTCGGGAGG CACACAGATC CTGAAGGAAT CATAAAATCC
    AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC TACACATCTG AAGCGGAGAT CACCCCGTCT
    TCCATGCCTG TGGTCACTGA CCCAGAAGCC TTTTCATCAG AGCACTTCAG CCTAGAGACC
    TATCGACAGA ATCTGCAGAC CACGCGCCTT GGGAAAGTGA TTCTGTTTGC AGAAGTGACC
    TCCACCACCA TGAGTCTCCT GGATGGGTTG ATGTTTGAGA TGCCGCAGGA AATGGGCTTA
    ATTGCCATTG CAGTTCGGCA GACCCAGGGC AAAGGAAGGG GCCCGAATGC CTGGCTGAGC
    CCCGTGGGAT GTGCTCTTTC CACTCTGCTG GTCTTCATCC CCCTGAGGTC ACAACTGGGA
    CAGAGGATTC CATTTGTCCA GCATTTGATG TCCTTGGCTG TGGTGGAGGC AGTGCGGTCT
    ATCCCAGGGT ATGAGGATAT CAACTTGCGA GTGAAGTGGC CCAACGATAT TTATTACAGC
    GATCTCATGA AGATCGGTGG GGTTCTGGTT AATTCAACAC TTATGGGAGA AACATTTTAT
    ATCCTCATCG GCTGTGGATT CAATGTGACG AACAGTAATC CTACCATTTG TATCAACGAC
    CTCATCGAAG AACACAATAA GCAGCACGGG GCGGGCCTGA AGCCCCTGCG AGCGGACTGT
    CTCATAGCCA GAGCCGTGAC CGTGCTGGAG AAACTGATCG ACAGGTTTCA GGACCAAGGG
    CCCGACGGCG TTCTTCCCCT CTATTATAAA TACTGGGTGC ACGGTGGTCA GCAAGTCCGC
    CTGGGCAGCA CGGAGGGGCC ACAGGCATCT ATTGTGGGCC TTGATGACTC CGGGTTCCTG
    CAGGTCCACC AAGAGGATGG TGGGGTTGTG ACGGTGCATC CAGATGGCAA CTCCTTCGAC
    ATGCTGAGAA ACCTCATCGT GCCCAAGCGA CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52629
    Clone ID Related Accession (Same CDS sequence) XM_006522853.4 , XM_006522853.3
    Accession Version XM_006522853.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522853.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCCTAG CACAGCCCCT CTCACCTCCC CAGTTCACTC TAGTTCTGCA GCCCAGGTCT 
    CTCCATCTCC AGAGCACACC TTCAATCTCC TGGCTCACCT TTCCCCCAGC CCCTCTTGGA
    AAAGCCAGCC TCCTGAGACC AGACAGTGAC CTCTCTTCCA ATGCCATCTT CAGTCCTGCC
    TGCCCTTTCC ATTCCTGTAG CCTCTCTTTA CCCGAGGCAC CCTCTCCATG GTCCTGGCGC
    CCCCAGATGC TACCCAGCCT GCTGCATCCT TCCACCTCTA GCGCATCCCT CTCCTGCATC
    CTTCCTTGCC CCGCACACCC GCTCCGCCCT CAGCCCCCCT CCTGCAGCGC ACGCGCACTC
    CACACTCCCG AAGCCTCGCT TCCGGCCGGG GCGGGGCCTG GCGCGAGCCG GGCGCGGGCG
    CCGGCCATGC TCATCACGCT GTGCTACCTG TACCTGTGGG CGCGCTGGGG GCGCCGGCCG
    GCCGCGCTGG TGCTGTCCAC GGTGCGGCGG CTGCGTGCTT CGCGCTGCTC CTTCACCTTC
    TGCCGCGCGG CCGCGCAGCC CAGGGACGCG CGCGTGTGCC TGAGCCAGGG CGGCCGCGTC
    TTCTGCGTCG GCGAGAGCCA GTCTGTTGAT GACTTGAACA AATGGGCCCT GTTTCTCGTG
    TCTCCCTTCA TACTTGAGGC AGAACATATA GCATTTGTGA CAGAGAGCAT TTGGATACAA
    GGTACCAGTT TACAAGAGCC ATCATCTTCA GAAACCGTTG TAAAATGGTC TGACTGCTGT
    CTGCCGTTAG CCTGCCGACC CGGGGACCCC TACCAGTTAA TTGCTAAGGC AAGCGTGGAC
    AACTTCAGCA AGCTTGGGGT GGCCTTCATG GAGGACAGGC TCCAGATGGA CAACGGCCTG
    ATAGCCCAGA AGATCGTTTC AGTCCACTTA AAGGACCCTG CTCTGAAGGA ACTCGGCAAA
    GCCTCCGACA AGCAAGTGCA GGGCCCGCCC CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG
    CCTGCACAAG GTGTCATGGA GCATGCTGGC CAAGGTGATT GCAAGGCCGC CGGTGAAGGC
    CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT GAAAGTGAGC CTGCTGCAGA TGGGGACCCA
    GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC CACCTGTCCA TCTGTCACGA GTGTCTGGAG
    CTCGAGAACA GTACCATTGA CTCGGTGAGG TCTGCGTCTG CGGAGAACAT TCCAGACCTT
    CCCTGTGATC ACAGCGGCGT GGAGGGTGCT GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG
    AAGAGAGTCA ACATTTCGGG AAAGGCGCCC AATATCTTGC TGTATGTGGG CTCTGGTTCC
    GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG CGATCTGTCC TGACTGACTG TGTGGACACG
    GACAGCTACA CTCTCTACCA CCTGCTGGAA GACAGCGCTC TGAGGGACCC GTGGTCTGAC
    AACTGCCTCC TGCTAGTTAT TGCCAGCAGG GACCCCATCC CCAAAGACAT ACAGCACAAG
    TTCATGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGGCC TGTCTTCCCC CTTCACGTTG
    GGTGGCTTTC GGGTGACCAG GAGAGATGTG CTACGGAACA CAGTCCAGAA CTTGGTTTTC
    TCTAAGGCTG ATGGGACTGA GGTACGGCTC AGTGTCCTGA GCAGCGGCTA CGTTTATGAA
    GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC CACCTGGAGA ATGAGGACAA GGATAAAATG
    ATTGTGCACG TGCCTTTTGG AACCCTCGGA GGGGAAGCCG TTCTCTGCCA GGTGCACCTG
    GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG ACTGCAGACG ACTTTAACGT GCTCAAATCC
    AGCAATGTGA GAAGACACGA GGTCCTTAAG GAGATCCTGA CTGCCCTCGG TCTGAGCTGT
    GACGCACCAC AGGTTCCTGC CTTAACGCCC CTGTACTTAC TGCTGGCCGC TGAGGAAACC
    CAGGATCCGT TCATGCAGTG GCTCGGGAGG CACACAGATC CTGAAGGAAT CATAAAATCC
    AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC TACACATCTG AAGCGGAGAT CACCCCGTCT
    TCCATGCCTG TGGTCACTGA CCCAGAAGCC TTTTCATCAG AGCACTTCAG CCTAGAGACC
    TATCGACAGA ATCTGCAGAC CACGCGCCTT GGGAAAGTGA TTCTGTTTGC AGAAGTGACC
    TCCACCACCA TGAGTCTCCT GGATGGGCTC CCGGGACTTC CCTCACAGTT GGCTTCCATG
    AGTCCCATCT ATCAGAGTTC ACTGGACAAT CTTTGGCTCC CTGCAGTGTT GTGGGTAATT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522916.1
    CDS301..2703
    Translation

    Target ORF information:

    RefSeq Version XM_006522853.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522853.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCCCTAG CACAGCCCCT CTCACCTCCC CAGTTCACTC TAGTTCTGCA GCCCAGGTCT 
    CTCCATCTCC AGAGCACACC TTCAATCTCC TGGCTCACCT TTCCCCCAGC CCCTCTTGGA
    AAAGCCAGCC TCCTGAGACC AGACAGTGAC CTCTCTTCCA ATGCCATCTT CAGTCCTGCC
    TGCCCTTTCC ATTCCTGTAG CCTCTCTTTA CCCGAGGCAC CCTCTCCATG GTCCTGGCGC
    CCCCAGATGC TACCCAGCCT GCTGCATCCT TCCACCTCTA GCGCATCCCT CTCCTGCATC
    CTTCCTTGCC CCGCACACCC GCTCCGCCCT CAGCCCCCCT CCTGCAGCGC ACGCGCACTC
    CACACTCCCG AAGCCTCGCT TCCGGCCGGG GCGGGGCCTG GCGCGAGCCG GGCGCGGGCG
    CCGGCCATGC TCATCACGCT GTGCTACCTG TACCTGTGGG CGCGCTGGGG GCGCCGGCCG
    GCCGCGCTGG TGCTGTCCAC GGTGCGGCGG CTGCGTGCTT CGCGCTGCTC CTTCACCTTC
    TGCCGCGCGG CCGCGCAGCC CAGGGACGCG CGCGTGTGCC TGAGCCAGGG CGGCCGCGTC
    TTCTGCGTCG GCGAGAGCCA GTCTGTTGAT GACTTGAACA AATGGGCCCT GTTTCTCGTG
    TCTCCCTTCA TACTTGAGGC AGAACATATA GCATTTGTGA CAGAGAGCAT TTGGATACAA
    GGTACCAGTT TACAAGAGCC ATCATCTTCA GAAACCGTTG TAAAATGGTC TGACTGCTGT
    CTGCCGTTAG CCTGCCGACC CGGGGACCCC TACCAGTTAA TTGCTAAGGC AAGCGTGGAC
    AACTTCAGCA AGCTTGGGGT GGCCTTCATG GAGGACAGGC TCCAGATGGA CAACGGCCTG
    ATAGCCCAGA AGATCGTTTC AGTCCACTTA AAGGACCCTG CTCTGAAGGA ACTCGGCAAA
    GCCTCCGACA AGCAAGTGCA GGGCCCGCCC CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG
    CCTGCACAAG GTGTCATGGA GCATGCTGGC CAAGGTGATT GCAAGGCCGC CGGTGAAGGC
    CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT GAAAGTGAGC CTGCTGCAGA TGGGGACCCA
    GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC CACCTGTCCA TCTGTCACGA GTGTCTGGAG
    CTCGAGAACA GTACCATTGA CTCGGTGAGG TCTGCGTCTG CGGAGAACAT TCCAGACCTT
    CCCTGTGATC ACAGCGGCGT GGAGGGTGCT GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG
    AAGAGAGTCA ACATTTCGGG AAAGGCGCCC AATATCTTGC TGTATGTGGG CTCTGGTTCC
    GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG CGATCTGTCC TGACTGACTG TGTGGACACG
    GACAGCTACA CTCTCTACCA CCTGCTGGAA GACAGCGCTC TGAGGGACCC GTGGTCTGAC
    AACTGCCTCC TGCTAGTTAT TGCCAGCAGG GACCCCATCC CCAAAGACAT ACAGCACAAG
    TTCATGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGGCC TGTCTTCCCC CTTCACGTTG
    GGTGGCTTTC GGGTGACCAG GAGAGATGTG CTACGGAACA CAGTCCAGAA CTTGGTTTTC
    TCTAAGGCTG ATGGGACTGA GGTACGGCTC AGTGTCCTGA GCAGCGGCTA CGTTTATGAA
    GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC CACCTGGAGA ATGAGGACAA GGATAAAATG
    ATTGTGCACG TGCCTTTTGG AACCCTCGGA GGGGAAGCCG TTCTCTGCCA GGTGCACCTG
    GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG ACTGCAGACG ACTTTAACGT GCTCAAATCC
    AGCAATGTGA GAAGACACGA GGTCCTTAAG GAGATCCTGA CTGCCCTCGG TCTGAGCTGT
    GACGCACCAC AGGTTCCTGC CTTAACGCCC CTGTACTTAC TGCTGGCCGC TGAGGAAACC
    CAGGATCCGT TCATGCAGTG GCTCGGGAGG CACACAGATC CTGAAGGAAT CATAAAATCC
    AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC TACACATCTG AAGCGGAGAT CACCCCGTCT
    TCCATGCCTG TGGTCACTGA CCCAGAAGCC TTTTCATCAG AGCACTTCAG CCTAGAGACC
    TATCGACAGA ATCTGCAGAC CACGCGCCTT GGGAAAGTGA TTCTGTTTGC AGAAGTGACC
    TCCACCACCA TGAGTCTCCT GGATGGGCTC CCGGGACTTC CCTCACAGTT GGCTTCCATG
    AGTCCCATCT ATCAGAGTTC ACTGGACAAT CTTTGGCTCC CTGCAGTGTT GTGGGTAATT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52630
    Clone ID Related Accession (Same CDS sequence) XM_006522854.4 , XM_006522854.3
    Accession Version XM_006522854.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product biotin--protein ligase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCTAG CACAGCCCCT CTCACCTCCC CAGTTCACTC TAGTTCTGCA GCCCAGGTCT 
    CTCCATCTCC AGAGCACACC TTCAATCTCC TGGCTCACCT TTCCCCCAGC CCCTCTTGGA
    AAAGCCAGCC TCCTGAGACC AGACAGTGAC CTCTCTTCCA ATGCCATCTT CAGTCCTGCC
    TGCCCTTTCC ATTCCTGTAG CCTCTCTTTA CCCGAGGCAC CCTCTCCATG GTCCTGGCGC
    CCCCAGATGC TACCCAGCCT GCTGCATCCT TCCACCTCTA GCGCATCCCT CTCCTGCATC
    CTTCCTTGCC CCGCACACCC GCTCCGCCCT CAGCCCCCCT CCTGCAGCGC ACGCGCACTC
    CACACTCCCG AAGCCTCGCT TCCGGCCGGG GCGGGGCCTG GCGCGAGCCG GGCGCGGGCG
    CCGGCCATGC TCATCACGCT GTGCTACCTG TACCTGTGGG CGCGCTGGGG GCGCCGGCCG
    GCCGCGCTGG TGCTGTCCAC GGTGCGGCGG CTGCGTGCTT CGCGCTGCTC CTTCACCTTC
    TGCCGCGCGG CCGCGCAGCC CAGGGACGCG CGCGTGTGCC TGAGCCAGGG CGGCCGCGTC
    TTCTGCGTCG GCGAGAGCCA GTCTGTTGAT GACTTGAACA AATGGGCCCT GTTTCTCGTG
    TCTCCCTTCA TACTTGAGGC AGAACATATA GCATTTGTGA CAGAGAGCAT TTGGATACAA
    GGTACCAGTT TACAAGAGCC ATCATCTTCA GAAACCGTTG TAAAATGGTC TGACTGCTGT
    CTGCCGTTAG CCTGCCGACC CGGGGACCCC TACCAGTTAA TTGCTAAGGC AAGCGTGGAC
    AACTTCAGCA AGCTTGGGGT GGCCTTCATG GAGGACAGGC TCCAGATGGA CAACGGCCTG
    ATAGCCCAGA AGATCGTTTC AGTCCACTTA AAGGACCCTG CTCTGAAGGA ACTCGGCAAA
    GCCTCCGACA AGCAAGTGCA GGGCCCGCCC CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG
    CCTGCACAAG GTGTCATGGA GCATGCTGGC CAAGGTGATT GCAAGGCCGC CGGTGAAGGC
    CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT GAAAGTGAGC CTGCTGCAGA TGGGGACCCA
    GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC CACCTGTCCA TCTGTCACGA GTGTCTGGAG
    CTCGAGAACA GTACCATTGA CTCGGTGAGG TCTGCGTCTG CGGAGAACAT TCCAGACCTT
    CCCTGTGATC ACAGCGGCGT GGAGGGTGCT GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG
    AAGAGAGTCA ACATTTCGGG AAAGGCGCCC AATATCTTGC TGTATGTGGG CTCTGGTTCC
    GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG CGATCTGTCC TGACTGACTG TGTGGACACG
    GACAGCTACA CTCTCTACCA CCTGCTGGAA GACAGCGCTC TGAGGGACCC GTGGTCTGAC
    AACTGCCTCC TGCTAGTTAT TGCCAGCAGG GACCCCATCC CCAAAGACAT ACAGCACAAG
    TTCATGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGGCC TGTCTTCCCC CTTCACGTTG
    GGTGGCTTTC GGGTGACCAG GAGAGATGTG CTACGGAACA CAGTCCAGAA CTTGGTTTTC
    TCTAAGGCTG ATGGGACTGA GGTACGGCTC AGTGTCCTGA GCAGCGGCTA CGTTTATGAA
    GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC CACCTGGAGA ATGAGGACAA GGATAAAATG
    ATTGTGCACG TGCCTTTTGG AACCCTCGGA GGGGAAGCCG TTCTCTGCCA GGTGCACCTG
    GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG ACTGCAGACG ACTTTAACGT GCTCAAATCC
    AGCAATGTGA GAAGACACGA GGTCCTTAAG GAGATCCTGA CTGCCCTCGG TCTGAGCTGT
    GACGCACCAC AGGTTCCTGC CTTAACGCCC CTGTACTTAC TGCTGGCCGC TGAGGAAACC
    CAGGATCCGT TCATGCAGTG GCTCGGGAGG CACACAGATC CTGAAGGAAT CATAAAATCC
    AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC TACACATCTG AAGCGGAGAT CACCCCGTCT
    TCCATGCCTG TGGTCACTGA CCCAGAAGCC TTTTCATCAG AGCACTTCAG CCTAGAGACC
    TATCGACAGA ATCTGCAGAC CACGCGCCTT GGGAAAGTGA TTCTGTTTGC AGAAGTGACC
    TCCACCACCA TGAGTCTCCT GGATGGAAGG AACGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522917.1
    CDS299..2617
    Translation

    Target ORF information:

    RefSeq Version XM_006522854.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522854.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCTAG CACAGCCCCT CTCACCTCCC CAGTTCACTC TAGTTCTGCA GCCCAGGTCT 
    CTCCATCTCC AGAGCACACC TTCAATCTCC TGGCTCACCT TTCCCCCAGC CCCTCTTGGA
    AAAGCCAGCC TCCTGAGACC AGACAGTGAC CTCTCTTCCA ATGCCATCTT CAGTCCTGCC
    TGCCCTTTCC ATTCCTGTAG CCTCTCTTTA CCCGAGGCAC CCTCTCCATG GTCCTGGCGC
    CCCCAGATGC TACCCAGCCT GCTGCATCCT TCCACCTCTA GCGCATCCCT CTCCTGCATC
    CTTCCTTGCC CCGCACACCC GCTCCGCCCT CAGCCCCCCT CCTGCAGCGC ACGCGCACTC
    CACACTCCCG AAGCCTCGCT TCCGGCCGGG GCGGGGCCTG GCGCGAGCCG GGCGCGGGCG
    CCGGCCATGC TCATCACGCT GTGCTACCTG TACCTGTGGG CGCGCTGGGG GCGCCGGCCG
    GCCGCGCTGG TGCTGTCCAC GGTGCGGCGG CTGCGTGCTT CGCGCTGCTC CTTCACCTTC
    TGCCGCGCGG CCGCGCAGCC CAGGGACGCG CGCGTGTGCC TGAGCCAGGG CGGCCGCGTC
    TTCTGCGTCG GCGAGAGCCA GTCTGTTGAT GACTTGAACA AATGGGCCCT GTTTCTCGTG
    TCTCCCTTCA TACTTGAGGC AGAACATATA GCATTTGTGA CAGAGAGCAT TTGGATACAA
    GGTACCAGTT TACAAGAGCC ATCATCTTCA GAAACCGTTG TAAAATGGTC TGACTGCTGT
    CTGCCGTTAG CCTGCCGACC CGGGGACCCC TACCAGTTAA TTGCTAAGGC AAGCGTGGAC
    AACTTCAGCA AGCTTGGGGT GGCCTTCATG GAGGACAGGC TCCAGATGGA CAACGGCCTG
    ATAGCCCAGA AGATCGTTTC AGTCCACTTA AAGGACCCTG CTCTGAAGGA ACTCGGCAAA
    GCCTCCGACA AGCAAGTGCA GGGCCCGCCC CCGGGCCCAG AAGCTTCCCC CGAGGCCCAG
    CCTGCACAAG GTGTCATGGA GCATGCTGGC CAAGGTGATT GCAAGGCCGC CGGTGAAGGC
    CCTTCCCCAC GGAGGAGGGG CTGTGCCCCT GAAAGTGAGC CTGCTGCAGA TGGGGACCCA
    GGGCTGAGCT CCCCGGAGCT CTGTCAGCTC CACCTGTCCA TCTGTCACGA GTGTCTGGAG
    CTCGAGAACA GTACCATTGA CTCGGTGAGG TCTGCGTCTG CGGAGAACAT TCCAGACCTT
    CCCTGTGATC ACAGCGGCGT GGAGGGTGCT GCGGGCGAAC TCTGCCCCGA ACGGAAAGGG
    AAGAGAGTCA ACATTTCGGG AAAGGCGCCC AATATCTTGC TGTATGTGGG CTCTGGTTCC
    GAGGAAGCCC TGGGCCGGCT CCAGCAGGTG CGATCTGTCC TGACTGACTG TGTGGACACG
    GACAGCTACA CTCTCTACCA CCTGCTGGAA GACAGCGCTC TGAGGGACCC GTGGTCTGAC
    AACTGCCTCC TGCTAGTTAT TGCCAGCAGG GACCCCATCC CCAAAGACAT ACAGCACAAG
    TTCATGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGGCC TGTCTTCCCC CTTCACGTTG
    GGTGGCTTTC GGGTGACCAG GAGAGATGTG CTACGGAACA CAGTCCAGAA CTTGGTTTTC
    TCTAAGGCTG ATGGGACTGA GGTACGGCTC AGTGTCCTGA GCAGCGGCTA CGTTTATGAA
    GAAGGCCCGA GCTTGGGCAG GCTCCAGGGC CACCTGGAGA ATGAGGACAA GGATAAAATG
    ATTGTGCACG TGCCTTTTGG AACCCTCGGA GGGGAAGCCG TTCTCTGCCA GGTGCACCTG
    GAGCTCCCGC CCGGCGCCTC CCTGGTGCAG ACTGCAGACG ACTTTAACGT GCTCAAATCC
    AGCAATGTGA GAAGACACGA GGTCCTTAAG GAGATCCTGA CTGCCCTCGG TCTGAGCTGT
    GACGCACCAC AGGTTCCTGC CTTAACGCCC CTGTACTTAC TGCTGGCCGC TGAGGAAACC
    CAGGATCCGT TCATGCAGTG GCTCGGGAGG CACACAGATC CTGAAGGAAT CATAAAATCC
    AGCAAGCTCT CCCTTCAGTT TGTTTCCTCC TACACATCTG AAGCGGAGAT CACCCCGTCT
    TCCATGCCTG TGGTCACTGA CCCAGAAGCC TTTTCATCAG AGCACTTCAG CCTAGAGACC
    TATCGACAGA ATCTGCAGAC CACGCGCCTT GGGAAAGTGA TTCTGTTTGC AGAAGTGACC
    TCCACCACCA TGAGTCTCCT GGATGGAAGG AACGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.