HLCS gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022413367.1	XP_022269075.1	biotin--protein ligase
XM_014109784.2	XP_013965259.1	biotin--protein ligase
XM_535590.5	XP_535590.3	biotin--protein ligase
XM_014109782.1	XP_013965257.1	biotin--protein ligase
XM_014109784.1	XP_013965259.1	biotin--protein ligase
XM_022413368.1	XP_022269076.1	biotin--protein ligase
XM_022413370.1	XP_022269078.1	biotin--protein ligase
XM_022413369.1	XP_022269077.1	biotin--protein ligase
XM_014109780.1	XP_013965255.1	biotin--protein ligase
XM_014109781.1	XP_013965256.1	biotin--protein ligase
XM_014109783.1	XP_013965258.1	biotin--protein ligase
XM_005638902.2	XP_005638959.1	biotin--protein ligase
XM_014109781.2	XP_013965256.1	biotin--protein ligase

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe29421	XM_022413367.1 Latest version!	Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29421	XM_014109780.1 Latest version!	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29421	XM_022413368.1 Latest version!	Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29421	XM_005638902.2 Latest version!	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29421	XM_014109781.2 Latest version!	Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29421	XM_014109784.2 Latest version!	Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29421	XM_022413369.1 Latest version!	Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29421	XM_014109783.1 Latest version!	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29421	XM_014109782.1 Latest version!	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29421	XM_535590.5 Latest version!	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29421	XM_022413370.1 Latest version!	Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OCe29421	XM_014109784.1	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29421	XM_014109781.1	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

HLCS ( XM_022413367.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022269075.1 Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_022413367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	biotin--protein ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269075.1
CDS	719..2893
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_022413367.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413367.1

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_014109784.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965259.1 Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_014109784.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	biotin--protein ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109784.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965259.1
CDS	374..2548
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_014109784.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109784.2

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_535590.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_535590.3 Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_535590.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	biotin--protein ligase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_535590.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_535590.3
CDS	1209..3383
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_535590.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_535590.5

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_014109782.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965257.1 Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_014109782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	biotin--protein ligase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965257.1
CDS	480..2654
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_014109782.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109782.1

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_014109784.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965259.1 Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_014109784.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	biotin--protein ligase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965259.1
CDS	291..2465
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_014109784.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109784.1

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_022413368.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022269076.1 Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_022413368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	biotin--protein ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269076.1
CDS	574..2748
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_022413368.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413368.1

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_022413370.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022269078.1 Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_022413370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	biotin--protein ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269078.1
CDS	403..2577
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_022413370.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413370.1

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_022413369.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022269077.1 Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_022413369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	biotin--protein ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269077.1
CDS	654..2828
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_022413369.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413369.1

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_014109780.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965255.1 Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_014109780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	biotin--protein ligase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965255.1
CDS	618..2792
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_014109780.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109780.1

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_014109781.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965256.1 Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.

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CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_014109781.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	biotin--protein ligase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965256.1
CDS	704..2878
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_014109781.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109781.1

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_014109783.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965258.1 Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X5, mRNA.

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CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_014109783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	biotin--protein ligase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965258.1
CDS	352..2526
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_014109783.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109783.1

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_005638902.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638959.1 Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X7, mRNA.

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CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_005638902.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	biotin--protein ligase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638959.1
CDS	580..2754
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_005638902.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638902.2

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_014109781.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965256.1 Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

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CloneID	OCe29421
Clone ID Related Accession (Same CDS sequence)	XM_022413367.1 , XM_014109784.2 , XM_535590.5 , XM_014109782.1 , XM_014109784.1 , XM_022413368.1 , XM_022413370.1 , XM_022413369.1 , XM_014109780.1 , XM_014109781.1 , XM_014109783.1 , XM_005638902.2 , XM_014109781.2
Accession Version	XM_014109781.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	biotin--protein ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965256.1
CDS	691..2865
Translation	MEDRLQMADGLIPQKIVSVHLQDCTLKALETSNKQAHILQQNPEPSTESKSEPDIMEYIDKDDPKVLRAHPTQEEAGTSGSEPAEEDDKGVGSVEHCPLHLSSCHECLELENSTIESVKFASAENIPDLPYDHGGLEDVADDSCPEREGRRVNITGKAPNILFYVGSTPQESLGRFQEVRSVLADCVDPDSYTLYHLSKESALRDPWSDNCLLLVIATREAIPADLSQKFMAYLSEGGKVLGLCSSFTLAGFQVMSKSTLKETVQNLVFSKADQTQVKLSILSSGFVYEDCSGGQLSPGQLQGHLENEDKDRLIVQVPFGTRGGEAVLCQAHLELPPSSSVVQGQEDFNLLKSSNLRRYEVLREILTTLGLNCDMKQIPALTPLYLLPVAEEILDPLMQWLGEHVDPEGVIKSSKLSLKFLSSYTSEIEITPSALPVVTDVGTFSSENFNLEIYRQNLQTKRLGKVILFAEVTPTTMNLLDGLMFEIPQEMGLIAIAVRQTQGKGRGANAWLSPMGCALSTLLISIPLRSQLGQRIPFIQHLMSLAVVEAVRSIPEYQDISLRVKWPNDIYYSDLMKLGGVLVNSTLLGETFYILIGCGFNVSNSNPTICINDLVMEYNKQHKAELKPLRADELIARTVTVLEKLIDTFQDKGPNGVLPLYYKYWVHSGQRVRLGGLEGPEVWIVGLDDSGFLQVQQENGDVVTVHPDGNSFDMLRNLIIPKQQ

Target ORF information:

RefSeq Version	XM_014109781.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109781.2

ORF Insert Sequence:

ATGGAAGATA GACTCCAGAT GGCTGATGGC CTGATACCTC AAAAGATTGT TTCAGTGCAT 
TTGCAGGACT GTACTCTGAA GGCGCTTGAG ACCTCAAACA AGCAAGCCCA TATCCTACAG 
CAGAATCCTG AACCTTCCAC CGAGAGTAAG TCAGAGCCGG ACATCATGGA GTACATCGAT 
AAAGATGACC CCAAGGTTCT TCGTGCCCAT CCCACACAGG AGGAAGCTGG TACCTCTGGC 
AGTGAGCCAG CTGAGGAGGA TGACAAAGGA GTAGGCTCCG TTGAGCACTG TCCCCTCCAT 
CTGTCTAGTT GCCATGAGTG TTTGGAGCTT GAGAATAGCA CCATTGAATC TGTCAAGTTT 
GCATCTGCAG AGAACATTCC AGATCTTCCC TACGATCACG GTGGTTTGGA GGATGTTGCT 
GATGACAGCT GCCCGGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTCTTCT ACGTGGGCTC TACACCCCAG GAGTCCCTGG GCAGGTTCCA GGAGGTCCGG 
TCCGTTCTGG CTGACTGTGT GGACCCCGAC AGCTATACAC TCTACCACCT GTCAAAGGAA 
AGTGCCCTCA GAGACCCATG GTCAGACAAC TGTCTATTGT TGGTCATTGC TACCAGGGAG 
GCTATTCCTG CAGACCTCTC CCAGAAGTTC ATGGCCTACC TTTCTGAAGG AGGGAAAGTG 
TTGGGCCTGT GTTCGTCCTT CACACTTGCT GGCTTCCAGG TGATGAGCAA GAGCACGCTC 
AAGGAAACAG TCCAGAACTT GGTTTTCTCC AAGGCCGACC AGACCCAGGT GAAGCTCAGC 
ATCCTGAGCA GCGGCTTCGT TTATGAGGAT TGCTCTGGAG GGCAGCTCAG CCCGGGCCAG 
CTCCAGGGTC ACCTGGAGAA TGAGGACAAA GACAGGCTGA TAGTGCAAGT GCCTTTTGGG 
ACACGTGGAG GAGAAGCCGT TCTTTGCCAG GCGCACCTAG AACTACCTCC CAGCTCTTCA 
GTGGTGCAAG GCCAGGAGGA TTTTAATCTG CTCAAATCAA GCAATCTCAG AAGATATGAA 
GTCCTTAGGG AGATCCTGAC AACGCTCGGC CTAAACTGTG ACATGAAACA AATTCCTGCC 
TTGACGCCTC TTTACTTACT GCCTGTGGCA GAGGAAATCT TGGACCCTCT TATGCAGTGG 
CTGGGAGAAC ATGTGGATCC TGAAGGAGTA ATAAAATCCA GCAAGCTGTC CCTTAAGTTT 
CTTTCATCCT ACACGTCTGA AATAGAAATC ACCCCATCTG CTCTACCTGT GGTCACAGAC 
GTGGGCACCT TCTCTTCAGA AAATTTTAAC CTAGAGATCT ACCGACAAAA TCTGCAGACA 
AAGAGACTTG GGAAAGTTAT TCTGTTTGCC GAGGTGACGC CCACCACGAT GAACCTTCTG 
GACGGGTTGA TGTTTGAGAT CCCGCAGGAA ATGGGGTTAA TTGCCATCGC AGTCCGCCAA 
ACCCAGGGCA AAGGGCGAGG AGCGAATGCT TGGCTGAGCC CCATGGGATG CGCGCTGTCC 
ACCCTGCTCA TCTCCATCCC ACTGAGATCC CAGCTGGGAC AGAGGATCCC GTTCATCCAG 
CATCTGATGT CCCTGGCCGT TGTGGAGGCA GTAAGGTCGA TTCCTGAGTA TCAGGATATC 
AGCTTACGAG TGAAGTGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCGACGCT TTTGGGAGAA ACATTTTATA TACTTATTGG CTGCGGATTT 
AACGTGAGTA ACAGTAACCC TACCATATGC ATCAATGATC TCGTCATGGA ATACAATAAG 
CAACACAAGG CAGAGCTGAA GCCCTTAAGA GCTGACGAGC TCATCGCCAG AACTGTGACT 
GTGCTGGAGA AGCTCATCGA CACGTTTCAG GACAAAGGGC CCAATGGCGT TCTTCCCCTT 
TATTATAAGT ATTGGGTACA CAGTGGCCAG CGGGTCCGCC TGGGAGGCCT CGAGGGGCCA 
GAAGTGTGGA TAGTCGGCCT GGATGACTCT GGCTTCCTTC AAGTCCAGCA GGAGAATGGC 
GACGTGGTGA CCGTGCATCC CGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTGATCATC 
CCCAAGCAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HLCS
Entrez Gene ID	478412
Full Name	holocarboxylase synthetase
General protein information	Preferred Names holocarboxylase synthetase Names biotin--protein ligase holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	HLCS	NP_000402.3
Arabidopsis thaliana (thale cress)	HCS1	NP_565605.1
Xenopus tropicalis (tropical clawed frog)	hlcs	XP_004912175.1
Pan troglodytes (chimpanzee)	HLCS	XP_003319106.2
	HLCS	XP_001084173.2
Bos taurus (cattle)	HLCS	XP_005202184.1
Arabidopsis thaliana (thale cress)	HCS2	NP_850957.1
Mus musculus (house mouse)	Hlcs	NP_631884.1
Gallus gallus (chicken)	HLCS	XP_004934596.1
Canis lupus familiaris (dog)	HLCS	XP_005638959.1
Rattus norvegicus (Norway rat)	Hlcs	XP_006248175.1
Caenorhabditis elegans (roundworm)	bpl-1	NP_871920.1
Danio rerio (zebrafish)	hlcs	XP_002663496.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HLCS cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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