HLCS cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	HLCS
Entrez Gene ID	418516
Full Name	holocarboxylase synthetase
General protein information	Preferred Names holocarboxylase synthetase Names biotin--protein ligase holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015301496.1	XP_015156982.1	biotin--protein ligase isoform X2
XM_416725.6	XP_416725.1	biotin--protein ligase isoform X1
XM_015301496.2	XP_015156982.1	biotin--protein ligase isoform X4
XM_025145239.1	XP_025001007.1	biotin--protein ligase isoform X4
XM_416725.5	XP_416725.1	biotin--protein ligase isoform X1
XM_025145233.1	XP_025001001.1	biotin--protein ligase isoform X2
XM_025145235.1	XP_025001003.1	biotin--protein ligase isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	HLCS	NP_000402.3
Arabidopsis thaliana (thale cress)	HCS1	NP_565605.1
Xenopus tropicalis (tropical clawed frog)	hlcs	XP_004912175.1
Pan troglodytes (chimpanzee)	HLCS	XP_003319106.2
	HLCS	XP_001084173.2
Bos taurus (cattle)	HLCS	XP_005202184.1
Arabidopsis thaliana (thale cress)	HCS2	NP_850957.1
Mus musculus (house mouse)	Hlcs	NP_631884.1
Gallus gallus (chicken)	HLCS	XP_004934596.1
Canis lupus familiaris (dog)	HLCS	XP_005638959.1
Rattus norvegicus (Norway rat)	Hlcs	XP_006248175.1
Caenorhabditis elegans (roundworm)	bpl-1	NP_871920.1
Danio rerio (zebrafish)	hlcs	XP_002663496.3

Gene Ontology

The following HLCS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLCS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa02722	XM_416725.6 Latest version!	Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OGa02723	XM_025145235.1 Latest version!	Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OGa142939	XM_025145233.1 Latest version!	Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa30361	XM_015301496.2 Latest version!	Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OGa30361	XM_025145239.1 Latest version!	Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OGa30361	XM_015301496.1	Gallus gallus holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OGa02722	XM_416725.5	Gallus gallus holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HLCS ( XM_416725.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_416725.1 Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa02722
Clone ID Related Accession (Same CDS sequence)	XM_416725.6 , XM_416725.5
Accession Version	XM_416725.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_416725.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATCA CGCTCTGCTA CCTCTACCTA TGGGCGCGCT GGGGGCCTTG CTCGGCCGCG 
CTCATCCGCC GCACCGTGCG CCGCCTCCAC CGCTCCCGTT GCTCCTTCAT CTTCTGCTGC 
TCCGCCGGCC CTTCGGCGCC TCCGGCTCAT CCGCCGCGCA GGCCGGCGCC TCCCGAGGAG 
CGCGTCTGCC TCAGGATAGG AAATAAAGTC TTCTTCACGG ACGAGACGCA GCTCCTCGAT 
GATTTAAACA GATGGAGTCT ACTTCTCATT TCTCCTTTTA TTTATCCTGA AAAGTTACTA 
GAAACAGAAC ATATAGCCTT TGTGACAGAA AGTGTTTCAG CTCAGGCAGA TAACTTGCAG 
AAATTACCTG TCTCTTCAAA AGAGATTGTG AAGTGGTCTG ACTATTGTTC ACCGTTGGCC 
TATAAACCTG GGGAACCTTA TAAGTTAATT GCTGAAGCAA GCGTAGATAA TTTCAGTAAC 
CTTGGAATAG CATTCATGGA AGACAGGCTT CAAATGGATA ACGGAATGGT TCCATGCAAG 
ATTGTTTCTG TCCATTTACA AGAAACTACT CTGAAGGAGT TAAAACAGGT GACACCATCT 
ATGAAAGAGA AGAGTGTAAG CATTACACAG ATGAATGAAG TAACTCCTGA TACTTTTGGG 
ACTACTGTTA AAACACAATT AGGAGTATCT GAGTCTGCAG GACAGGGTGA TTCAGAGGCA 
AAAGAAGAGA AATCTAAGCT AGTGAAAGAA AATGGAGATG AATCCAACAG CCTATCTGAC 
AAAGAGACAG GTTTTGGAGA ACATCATCAC TTGCATCTTT CCAGCTGTCG TGAATGTCTG 
CAACTTGAAA ACAGCACTAT TGAATCAGTC AGATTTGCCT CTGCGGAAGA CATTCCAGAA 
CTTCCTGATG ATTGCAATAG CAAACTGGAA GAGAATGGTG ATTGTCTGAC TACAGGAATA 
AAGAGAGTAA ACCTTGCTGG AAAGCCTCCT AATATATTGA TTTATGTTGG CTCTGGAACT 
GCAAAAGTGG ACTTTGAGCA AGTAAAATCA ATCATTCAGG AATGTGTTGA TACAGACAGC 
TATACCATCT ATCAGCTGCA TGAGGAACAA GTTCTGAAAG CTCCGTGGAT TGATAATTCA 
CTGCTGTTGA TCATTGCCAC AGAGGGGCCT ATTTCTGAGA AGAACCAAAA ACAGTTTATG 
AAGTTTTTAT CTAAAGGTGG AAAGATTCTA GGGTTATCTT CCTCTTTTAC ATTTGATGGT 
ATACAAATAA AGAGGAAAGA CAAACTGAAG AGAACTGTTC ATGAATTAGT GGTCTCTAAA 
ATGGACAGTA CTGAAATGAA GTTAAATCTT TTGATCAGTG GCTGTATTTT TGAGGTAGCG 
ATGAAGGAAG GGAGCAGCAA AGTGAAGCCA TTGAGTCGCT TAAATAATGC TGATAAAGAT 
ATAGTTATTG TTTATCTGCC TTATGGAGAC AATGGAGGAG AAGCCATTCT TTCTCAGGTG 
CATTTGGAAC TGGATACCAA TTCTGTGGAT ATCCAAACTG AAGAGGATTT TAATTTGCTT 
AAGATGAGCA ATCCCAAGAG ATATGAAGTT CTCAAGGAGA TTCTGATATC ACTTGGGCTG 
AGTTGTGAAT TAAGTGAGAT TCCTATGTTG ACGCCTATTT ACCTTCTCTC TTCTGATGAG 
GAAATCCATC TTACGTTTTT GAAATGGCTT GAGGAGAATG TAAATGTTGA AGGATTAATA 
GAATCCAGCA AAGTGTCTCT TAAATTTGTT TCATCTTGTG AATCGAAAAT GGAGATAACT 
CCATCTCTCA TGTCAGTCAT CACCAAGATG GGAAACTTCT CATCAGAACA TTTCAGCCTG 
GAGACATATC AGCAGAATCT ACAAACAAAG AAACTTGGAA AGATTCTTTT CTTTACTGAA 
GTTACCACCA CAACAATGAA TCTTCTAGAC GGGTTAATGT TCAAGTTGCC TGAGGAGATG 
GGTTTAATAG CAATTGCTGT TCGACAAACA CAAGGGAAAG GTCGTGGCGG AAATGTTTGG 
CTCAGTCCCA TTGGTTGTGC ACTATCCACT TTACATATCA CAATCCCTTT ACATTCCAAC 
TTGGGCCAGA GAATTCCTTT TATTCAACAC CTAGTGTCCT TGGCAGTGGT AGAGTCGGTT 
AGATCAATAC CAGGATATGA GGATATTGAC CTGCGAGTAA AATGGCCAAA TGATATTTAT 
TACAGTGATC TTATGAAGCT TGGTGGAGTT CTGGTAACCT CTACACTTAT TGAGACAACA 
TTTCATATAC TTATTGGCTT TGGATTTAAT GTGAATAACA GCAACCCCAC CATATGCATC 
AATGACCTCA TCACAAAATT TAATAAGGAA GAGGGGACAA ATCTGAAGGC TTTAACTGCA 
GACTGCCTTA TTGCGAGAAC TGTAACTGTG CTAGAGAGGC TTATAGATAT TTTTCAGGAG 
AAAGGGCCCA ATGGAGTTCT TCCTCGTTAC TACAAGTACT GGGTACACAG TGGTAAGCAG 
GTTCGTCTCC ACAATGAGGA GGGCCCGTTG GCATGGATAG TTGGGATTGA TGATTATGGA 
TTCCTTCAAG TTCATGAAGA AGGCAAAGGT GTAGAGTCTG TGCATCCAGA TGGCAACTCT 
TTTGACATGC TGAAAAACCT CATAATCCCA AAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_416725.1
CDS	40..2718
Translation	MLITLCYLYLWARWGPCSAALIRRTVRRLHRSRCSFIFCCSAGPSAPPAHPPRRPAPPEERVCLRIGNKVFFTDETQLLDDLNRWSLLLISPFIYPEKLLETEHIAFVTESVSAQADNLQKLPVSSKEIVKWSDYCSPLAYKPGEPYKLIAEASVDNFSNLGIAFMEDRLQMDNGMVPCKIVSVHLQETTLKELKQVTPSMKEKSVSITQMNEVTPDTFGTTVKTQLGVSESAGQGDSEAKEEKSKLVKENGDESNSLSDKETGFGEHHHLHLSSCRECLQLENSTIESVRFASAEDIPELPDDCNSKLEENGDCLTTGIKRVNLAGKPPNILIYVGSGTAKVDFEQVKSIIQECVDTDSYTIYQLHEEQVLKAPWIDNSLLLIIATEGPISEKNQKQFMKFLSKGGKILGLSSSFTFDGIQIKRKDKLKRTVHELVVSKMDSTEMKLNLLISGCIFEVAMKEGSSKVKPLSRLNNADKDIVIVYLPYGDNGGEAILSQVHLELDTNSVDIQTEEDFNLLKMSNPKRYEVLKEILISLGLSCELSEIPMLTPIYLLSSDEEIHLTFLKWLEENVNVEGLIESSKVSLKFVSSCESKMEITPSLMSVITKMGNFSSEHFSLETYQQNLQTKKLGKILFFTEVTTTTMNLLDGLMFKLPEEMGLIAIAVRQTQGKGRGGNVWLSPIGCALSTLHITIPLHSNLGQRIPFIQHLVSLAVVESVRSIPGYEDIDLRVKWPNDIYYSDLMKLGGVLVTSTLIETTFHILIGFGFNVNNSNPTICINDLITKFNKEEGTNLKALTADCLIARTVTVLERLIDIFQEKGPNGVLPRYYKYWVHSGKQVRLHNEEGPLAWIVGIDDYGFLQVHEEGKGVESVHPDGNSFDMLKNLIIPKH

Target ORF information:

RefSeq Version	XM_416725.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_416725.6

ORF Insert Sequence:

ATGCTGATCA CGCTCTGCTA CCTCTACCTA TGGGCGCGCT GGGGGCCTTG CTCGGCCGCG 
CTCATCCGCC GCACCGTGCG CCGCCTCCAC CGCTCCCGTT GCTCCTTCAT CTTCTGCTGC 
TCCGCCGGCC CTTCGGCGCC TCCGGCTCAT CCGCCGCGCA GGCCGGCGCC TCCCGAGGAG 
CGCGTCTGCC TCAGGATAGG AAATAAAGTC TTCTTCACGG ACGAGACGCA GCTCCTCGAT 
GATTTAAACA GATGGAGTCT ACTTCTCATT TCTCCTTTTA TTTATCCTGA AAAGTTACTA 
GAAACAGAAC ATATAGCCTT TGTGACAGAA AGTGTTTCAG CTCAGGCAGA TAACTTGCAG 
AAATTACCTG TCTCTTCAAA AGAGATTGTG AAGTGGTCTG ACTATTGTTC ACCGTTGGCC 
TATAAACCTG GGGAACCTTA TAAGTTAATT GCTGAAGCAA GCGTAGATAA TTTCAGTAAC 
CTTGGAATAG CATTCATGGA AGACAGGCTT CAAATGGATA ACGGAATGGT TCCATGCAAG 
ATTGTTTCTG TCCATTTACA AGAAACTACT CTGAAGGAGT TAAAACAGGT GACACCATCT 
ATGAAAGAGA AGAGTGTAAG CATTACACAG ATGAATGAAG TAACTCCTGA TACTTTTGGG 
ACTACTGTTA AAACACAATT AGGAGTATCT GAGTCTGCAG GACAGGGTGA TTCAGAGGCA 
AAAGAAGAGA AATCTAAGCT AGTGAAAGAA AATGGAGATG AATCCAACAG CCTATCTGAC 
AAAGAGACAG GTTTTGGAGA ACATCATCAC TTGCATCTTT CCAGCTGTCG TGAATGTCTG 
CAACTTGAAA ACAGCACTAT TGAATCAGTC AGATTTGCCT CTGCGGAAGA CATTCCAGAA 
CTTCCTGATG ATTGCAATAG CAAACTGGAA GAGAATGGTG ATTGTCTGAC TACAGGAATA 
AAGAGAGTAA ACCTTGCTGG AAAGCCTCCT AATATATTGA TTTATGTTGG CTCTGGAACT 
GCAAAAGTGG ACTTTGAGCA AGTAAAATCA ATCATTCAGG AATGTGTTGA TACAGACAGC 
TATACCATCT ATCAGCTGCA TGAGGAACAA GTTCTGAAAG CTCCGTGGAT TGATAATTCA 
CTGCTGTTGA TCATTGCCAC AGAGGGGCCT ATTTCTGAGA AGAACCAAAA ACAGTTTATG 
AAGTTTTTAT CTAAAGGTGG AAAGATTCTA GGGTTATCTT CCTCTTTTAC ATTTGATGGT 
ATACAAATAA AGAGGAAAGA CAAACTGAAG AGAACTGTTC ATGAATTAGT GGTCTCTAAA 
ATGGACAGTA CTGAAATGAA GTTAAATCTT TTGATCAGTG GCTGTATTTT TGAGGTAGCG 
ATGAAGGAAG GGAGCAGCAA AGTGAAGCCA TTGAGTCGCT TAAATAATGC TGATAAAGAT 
ATAGTTATTG TTTATCTGCC TTATGGAGAC AATGGAGGAG AAGCCATTCT TTCTCAGGTG 
CATTTGGAAC TGGATACCAA TTCTGTGGAT ATCCAAACTG AAGAGGATTT TAATTTGCTT 
AAGATGAGCA ATCCCAAGAG ATATGAAGTT CTCAAGGAGA TTCTGATATC ACTTGGGCTG 
AGTTGTGAAT TAAGTGAGAT TCCTATGTTG ACGCCTATTT ACCTTCTCTC TTCTGATGAG 
GAAATCCATC TTACGTTTTT GAAATGGCTT GAGGAGAATG TAAATGTTGA AGGATTAATA 
GAATCCAGCA AAGTGTCTCT TAAATTTGTT TCATCTTGTG AATCGAAAAT GGAGATAACT 
CCATCTCTCA TGTCAGTCAT CACCAAGATG GGAAACTTCT CATCAGAACA TTTCAGCCTG 
GAGACATATC AGCAGAATCT ACAAACAAAG AAACTTGGAA AGATTCTTTT CTTTACTGAA 
GTTACCACCA CAACAATGAA TCTTCTAGAC GGGTTAATGT TCAAGTTGCC TGAGGAGATG 
GGTTTAATAG CAATTGCTGT TCGACAAACA CAAGGGAAAG GTCGTGGCGG AAATGTTTGG 
CTCAGTCCCA TTGGTTGTGC ACTATCCACT TTACATATCA CAATCCCTTT ACATTCCAAC 
TTGGGCCAGA GAATTCCTTT TATTCAACAC CTAGTGTCCT TGGCAGTGGT AGAGTCGGTT 
AGATCAATAC CAGGATATGA GGATATTGAC CTGCGAGTAA AATGGCCAAA TGATATTTAT 
TACAGTGATC TTATGAAGCT TGGTGGAGTT CTGGTAACCT CTACACTTAT TGAGACAACA 
TTTCATATAC TTATTGGCTT TGGATTTAAT GTGAATAACA GCAACCCCAC CATATGCATC 
AATGACCTCA TCACAAAATT TAATAAGGAA GAGGGGACAA ATCTGAAGGC TTTAACTGCA 
GACTGCCTTA TTGCGAGAAC TGTAACTGTG CTAGAGAGGC TTATAGATAT TTTTCAGGAG 
AAAGGGCCCA ATGGAGTTCT TCCTCGTTAC TACAAGTACT GGGTACACAG TGGTAAGCAG 
GTTCGTCTCC ACAATGAGGA GGGCCCGTTG GCATGGATAG TTGGGATTGA TGATTATGGA 
TTCCTTCAAG TTCATGAAGA AGGCAAAGGT GTAGAGTCTG TGCATCCAGA TGGCAACTCT 
TTTGACATGC TGAAAAACCT CATAATCCCA AAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_416725.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_416725.1 Gallus gallus holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa02722
Clone ID Related Accession (Same CDS sequence)	XM_416725.6 , XM_416725.5
Accession Version	XM_416725.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455738.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_416725.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATCA CGCTCTGCTA CCTCTACCTA TGGGCGCGCT GGGGGCCTTG CTCGGCCGCG 
CTCATCCGCC GCACCGTGCG CCGCCTCCAC CGCTCCCGTT GCTCCTTCAT CTTCTGCTGC 
TCCGCCGGCC CTTCGGCGCC TCCGGCTCAT CCGCCGCGCA GGCCGGCGCC TCCCGAGGAG 
CGCGTCTGCC TCAGGATAGG AAATAAAGTC TTCTTCACGG ACGAGACGCA GCTCCTCGAT 
GATTTAAACA GATGGAGTCT ACTTCTCATT TCTCCTTTTA TTTATCCTGA AAAGTTACTA 
GAAACAGAAC ATATAGCCTT TGTGACAGAA AGTGTTTCAG CTCAGGCAGA TAACTTGCAG 
AAATTACCTG TCTCTTCAAA AGAGATTGTG AAGTGGTCTG ACTATTGTTC ACCGTTGGCC 
TATAAACCTG GGGAACCTTA TAAGTTAATT GCTGAAGCAA GCGTAGATAA TTTCAGTAAC 
CTTGGAATAG CATTCATGGA AGACAGGCTT CAAATGGATA ACGGAATGGT TCCATGCAAG 
ATTGTTTCTG TCCATTTACA AGAAACTACT CTGAAGGAGT TAAAACAGGT GACACCATCT 
ATGAAAGAGA AGAGTGTAAG CATTACACAG ATGAATGAAG TAACTCCTGA TACTTTTGGG 
ACTACTGTTA AAACACAATT AGGAGTATCT GAGTCTGCAG GACAGGGTGA TTCAGAGGCA 
AAAGAAGAGA AATCTAAGCT AGTGAAAGAA AATGGAGATG AATCCAACAG CCTATCTGAC 
AAAGAGACAG GTTTTGGAGA ACATCATCAC TTGCATCTTT CCAGCTGTCG TGAATGTCTG 
CAACTTGAAA ACAGCACTAT TGAATCAGTC AGATTTGCCT CTGCGGAAGA CATTCCAGAA 
CTTCCTGATG ATTGCAATAG CAAACTGGAA GAGAATGGTG ATTGTCTGAC TACAGGAATA 
AAGAGAGTAA ACCTTGCTGG AAAGCCTCCT AATATATTGA TTTATGTTGG CTCTGGAACT 
GCAAAAGTGG ACTTTGAGCA AGTAAAATCA ATCATTCAGG AATGTGTTGA TACAGACAGC 
TATACCATCT ATCAGCTGCA TGAGGAACAA GTTCTGAAAG CTCCGTGGAT TGATAATTCA 
CTGCTGTTGA TCATTGCCAC AGAGGGGCCT ATTTCTGAGA AGAACCAAAA ACAGTTTATG 
AAGTTTTTAT CTAAAGGTGG AAAGATTCTA GGGTTATCTT CCTCTTTTAC ATTTGATGGT 
ATACAAATAA AGAGGAAAGA CAAACTGAAG AGAACTGTTC ATGAATTAGT GGTCTCTAAA 
ATGGACAGTA CTGAAATGAA GTTAAATCTT TTGATCAGTG GCTGTATTTT TGAGGTAGCG 
ATGAAGGAAG GGAGCAGCAA AGTGAAGCCA TTGAGTCGCT TAAATAATGC TGATAAAGAT 
ATAGTTATTG TTTATCTGCC TTATGGAGAC AATGGAGGAG AAGCCATTCT TTCTCAGGTG 
CATTTGGAAC TGGATACCAA TTCTGTGGAT ATCCAAACTG AAGAGGATTT TAATTTGCTT 
AAGATGAGCA ATCCCAAGAG ATATGAAGTT CTCAAGGAGA TTCTGATATC ACTTGGGCTG 
AGTTGTGAAT TAAGTGAGAT TCCTATGTTG ACGCCTATTT ACCTTCTCTC TTCTGATGAG 
GAAATCCATC TTACGTTTTT GAAATGGCTT GAGGAGAATG TAAATGTTGA AGGATTAATA 
GAATCCAGCA AAGTGTCTCT TAAATTTGTT TCATCTTGTG AATCGAAAAT GGAGATAACT 
CCATCTCTCA TGTCAGTCAT CACCAAGATG GGAAACTTCT CATCAGAACA TTTCAGCCTG 
GAGACATATC AGCAGAATCT ACAAACAAAG AAACTTGGAA AGATTCTTTT CTTTACTGAA 
GTTACCACCA CAACAATGAA TCTTCTAGAC GGGTTAATGT TCAAGTTGCC TGAGGAGATG 
GGTTTAATAG CAATTGCTGT TCGACAAACA CAAGGGAAAG GTCGTGGCGG AAATGTTTGG 
CTCAGTCCCA TTGGTTGTGC ACTATCCACT TTACATATCA CAATCCCTTT ACATTCCAAC 
TTGGGCCAGA GAATTCCTTT TATTCAACAC CTAGTGTCCT TGGCAGTGGT AGAGTCGGTT 
AGATCAATAC CAGGATATGA GGATATTGAC CTGCGAGTAA AATGGCCAAA TGATATTTAT 
TACAGTGATC TTATGAAGCT TGGTGGAGTT CTGGTAACCT CTACACTTAT TGAGACAACA 
TTTCATATAC TTATTGGCTT TGGATTTAAT GTGAATAACA GCAACCCCAC CATATGCATC 
AATGACCTCA TCACAAAATT TAATAAGGAA GAGGGGACAA ATCTGAAGGC TTTAACTGCA 
GACTGCCTTA TTGCGAGAAC TGTAACTGTG CTAGAGAGGC TTATAGATAT TTTTCAGGAG 
AAAGGGCCCA ATGGAGTTCT TCCTCGTTAC TACAAGTACT GGGTACACAG TGGTAAGCAG 
GTTCGTCTCC ACAATGAGGA GGGCCCGTTG GCATGGATAG TTGGGATTGA TGATTATGGA 
TTCCTTCAAG TTCATGAAGA AGGCAAAGGT GTAGAGTCTG TGCATCCAGA TGGCAACTCT 
TTTGACATGC TGAAAAACCT CATAATCCCA AAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_416725.1
CDS	38..2716
Translation	MLITLCYLYLWARWGPCSAALIRRTVRRLHRSRCSFIFCCSAGPSAPPAHPPRRPAPPEERVCLRIGNKVFFTDETQLLDDLNRWSLLLISPFIYPEKLLETEHIAFVTESVSAQADNLQKLPVSSKEIVKWSDYCSPLAYKPGEPYKLIAEASVDNFSNLGIAFMEDRLQMDNGMVPCKIVSVHLQETTLKELKQVTPSMKEKSVSITQMNEVTPDTFGTTVKTQLGVSESAGQGDSEAKEEKSKLVKENGDESNSLSDKETGFGEHHHLHLSSCRECLQLENSTIESVRFASAEDIPELPDDCNSKLEENGDCLTTGIKRVNLAGKPPNILIYVGSGTAKVDFEQVKSIIQECVDTDSYTIYQLHEEQVLKAPWIDNSLLLIIATEGPISEKNQKQFMKFLSKGGKILGLSSSFTFDGIQIKRKDKLKRTVHELVVSKMDSTEMKLNLLISGCIFEVAMKEGSSKVKPLSRLNNADKDIVIVYLPYGDNGGEAILSQVHLELDTNSVDIQTEEDFNLLKMSNPKRYEVLKEILISLGLSCELSEIPMLTPIYLLSSDEEIHLTFLKWLEENVNVEGLIESSKVSLKFVSSCESKMEITPSLMSVITKMGNFSSEHFSLETYQQNLQTKKLGKILFFTEVTTTTMNLLDGLMFKLPEEMGLIAIAVRQTQGKGRGGNVWLSPIGCALSTLHITIPLHSNLGQRIPFIQHLVSLAVVESVRSIPGYEDIDLRVKWPNDIYYSDLMKLGGVLVTSTLIETTFHILIGFGFNVNNSNPTICINDLITKFNKEEGTNLKALTADCLIARTVTVLERLIDIFQEKGPNGVLPRYYKYWVHSGKQVRLHNEEGPLAWIVGIDDYGFLQVHEEGKGVESVHPDGNSFDMLKNLIIPKH

Target ORF information:

RefSeq Version	XM_416725.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_416725.5

ORF Insert Sequence:

ATGCTGATCA CGCTCTGCTA CCTCTACCTA TGGGCGCGCT GGGGGCCTTG CTCGGCCGCG 
CTCATCCGCC GCACCGTGCG CCGCCTCCAC CGCTCCCGTT GCTCCTTCAT CTTCTGCTGC 
TCCGCCGGCC CTTCGGCGCC TCCGGCTCAT CCGCCGCGCA GGCCGGCGCC TCCCGAGGAG 
CGCGTCTGCC TCAGGATAGG AAATAAAGTC TTCTTCACGG ACGAGACGCA GCTCCTCGAT 
GATTTAAACA GATGGAGTCT ACTTCTCATT TCTCCTTTTA TTTATCCTGA AAAGTTACTA 
GAAACAGAAC ATATAGCCTT TGTGACAGAA AGTGTTTCAG CTCAGGCAGA TAACTTGCAG 
AAATTACCTG TCTCTTCAAA AGAGATTGTG AAGTGGTCTG ACTATTGTTC ACCGTTGGCC 
TATAAACCTG GGGAACCTTA TAAGTTAATT GCTGAAGCAA GCGTAGATAA TTTCAGTAAC 
CTTGGAATAG CATTCATGGA AGACAGGCTT CAAATGGATA ACGGAATGGT TCCATGCAAG 
ATTGTTTCTG TCCATTTACA AGAAACTACT CTGAAGGAGT TAAAACAGGT GACACCATCT 
ATGAAAGAGA AGAGTGTAAG CATTACACAG ATGAATGAAG TAACTCCTGA TACTTTTGGG 
ACTACTGTTA AAACACAATT AGGAGTATCT GAGTCTGCAG GACAGGGTGA TTCAGAGGCA 
AAAGAAGAGA AATCTAAGCT AGTGAAAGAA AATGGAGATG AATCCAACAG CCTATCTGAC 
AAAGAGACAG GTTTTGGAGA ACATCATCAC TTGCATCTTT CCAGCTGTCG TGAATGTCTG 
CAACTTGAAA ACAGCACTAT TGAATCAGTC AGATTTGCCT CTGCGGAAGA CATTCCAGAA 
CTTCCTGATG ATTGCAATAG CAAACTGGAA GAGAATGGTG ATTGTCTGAC TACAGGAATA 
AAGAGAGTAA ACCTTGCTGG AAAGCCTCCT AATATATTGA TTTATGTTGG CTCTGGAACT 
GCAAAAGTGG ACTTTGAGCA AGTAAAATCA ATCATTCAGG AATGTGTTGA TACAGACAGC 
TATACCATCT ATCAGCTGCA TGAGGAACAA GTTCTGAAAG CTCCGTGGAT TGATAATTCA 
CTGCTGTTGA TCATTGCCAC AGAGGGGCCT ATTTCTGAGA AGAACCAAAA ACAGTTTATG 
AAGTTTTTAT CTAAAGGTGG AAAGATTCTA GGGTTATCTT CCTCTTTTAC ATTTGATGGT 
ATACAAATAA AGAGGAAAGA CAAACTGAAG AGAACTGTTC ATGAATTAGT GGTCTCTAAA 
ATGGACAGTA CTGAAATGAA GTTAAATCTT TTGATCAGTG GCTGTATTTT TGAGGTAGCG 
ATGAAGGAAG GGAGCAGCAA AGTGAAGCCA TTGAGTCGCT TAAATAATGC TGATAAAGAT 
ATAGTTATTG TTTATCTGCC TTATGGAGAC AATGGAGGAG AAGCCATTCT TTCTCAGGTG 
CATTTGGAAC TGGATACCAA TTCTGTGGAT ATCCAAACTG AAGAGGATTT TAATTTGCTT 
AAGATGAGCA ATCCCAAGAG ATATGAAGTT CTCAAGGAGA TTCTGATATC ACTTGGGCTG 
AGTTGTGAAT TAAGTGAGAT TCCTATGTTG ACGCCTATTT ACCTTCTCTC TTCTGATGAG 
GAAATCCATC TTACGTTTTT GAAATGGCTT GAGGAGAATG TAAATGTTGA AGGATTAATA 
GAATCCAGCA AAGTGTCTCT TAAATTTGTT TCATCTTGTG AATCGAAAAT GGAGATAACT 
CCATCTCTCA TGTCAGTCAT CACCAAGATG GGAAACTTCT CATCAGAACA TTTCAGCCTG 
GAGACATATC AGCAGAATCT ACAAACAAAG AAACTTGGAA AGATTCTTTT CTTTACTGAA 
GTTACCACCA CAACAATGAA TCTTCTAGAC GGGTTAATGT TCAAGTTGCC TGAGGAGATG 
GGTTTAATAG CAATTGCTGT TCGACAAACA CAAGGGAAAG GTCGTGGCGG AAATGTTTGG 
CTCAGTCCCA TTGGTTGTGC ACTATCCACT TTACATATCA CAATCCCTTT ACATTCCAAC 
TTGGGCCAGA GAATTCCTTT TATTCAACAC CTAGTGTCCT TGGCAGTGGT AGAGTCGGTT 
AGATCAATAC CAGGATATGA GGATATTGAC CTGCGAGTAA AATGGCCAAA TGATATTTAT 
TACAGTGATC TTATGAAGCT TGGTGGAGTT CTGGTAACCT CTACACTTAT TGAGACAACA 
TTTCATATAC TTATTGGCTT TGGATTTAAT GTGAATAACA GCAACCCCAC CATATGCATC 
AATGACCTCA TCACAAAATT TAATAAGGAA GAGGGGACAA ATCTGAAGGC TTTAACTGCA 
GACTGCCTTA TTGCGAGAAC TGTAACTGTG CTAGAGAGGC TTATAGATAT TTTTCAGGAG 
AAAGGGCCCA ATGGAGTTCT TCCTCGTTAC TACAAGTACT GGGTACACAG TGGTAAGCAG 
GTTCGTCTCC ACAATGAGGA GGGCCCGTTG GCATGGATAG TTGGGATTGA TGATTATGGA 
TTCCTTCAAG TTCATGAAGA AGGCAAAGGT GTAGAGTCTG TGCATCCAGA TGGCAACTCT 
TTTGACATGC TGAAAAACCT CATAATCCCA AAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_025145235.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001003.1 Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa02723
Clone ID Related Accession (Same CDS sequence)	XM_025145235.1
Accession Version	XM_025145235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	biotin--protein ligase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTCC TCGATGATTT AAACAGATGG AGTCTACTTC TCATTTCTCC TTTTATTTAT 
CCTGAAAAGT TACTAGAAAC AGAACATATA GCCTTTGTGA CAGAAAGTGT TTCAGCTCAG 
GCAGATAACT TGCAGAAATT ACCTGTCTCT TCAAAAGAGA TTGTGAAGTG GTCTGACTAT 
TGTTCACCGT TGGCCTATAA ACCTGGGGAA CCTTATAAGT TAATTGCTGA AGCAAGCGTA 
GATAATTTCA GTAACCTTGG AATAGCATTC ATGGAAGACA GGCTTCAAAT GGATAACGGA 
ATGGTTCCAT GCAAGATTGT TTCTGTCCAT TTACAAGAAA CTACTCTGAA GGAGTTAAAA 
CAGGTGACAC CATCTATGAA AGAGAAGAGT GTAAGCATTA CACAGATGAA TGAAGTAACT 
CCTGATACTT TTGGGACTAC TGTTAAAACA CAATTAGGAG TATCTGAGTC TGCAGGACAG 
GGTGATTCAG AGGCAAAAGA AGAGAAATCT AAGCTAGTGA AAGAAAATGG AGATGAATCC 
AACAGCCTAT CTGACAAAGA GACAGGTTTT GGAGAACATC ATCACTTGCA TCTTTCCAGC 
TGTCGTGAAT GTCTGCAACT TGAAAACAGC ACTATTGAAT CAGTCAGATT TGCCTCTGCG 
GAAGACATTC CAGAACTTCC TGATGATTGC AATAGCAAAC TGGAAGAGAA TGGTGATTGT 
CTGACTACAG GAATAAAGAG AGTAAACCTT GCTGGAAAGC CTCCTAATAT ATTGATTTAT 
GTTGGCTCTG GAACTGCAAA AGTGGACTTT GAGCAAGTAA AATCAATCAT TCAGGAATGT 
GTTGATACAG ACAGCTATAC CATCTATCAG CTGCATGAGG AACAAGTTCT GAAAGCTCCG 
TGGATTGATA ATTCACTGCT GTTGATCATT GCCACAGAGG GGCCTATTTC TGAGAAGAAC 
CAAAAACAGT TTATGAAGTT TTTATCTAAA GGTGGAAAGA TTCTAGGGTT ATCTTCCTCT 
TTTACATTTG ATGGTATACA AATAAAGAGG AAAGACAAAC TGAAGAGAAC TGTTCATGAA 
TTAGTGGTCT CTAAAATGGA CAGTACTGAA ATGAAGTTAA ATCTTTTGAT CAGTGGCTGT 
ATTTTTGAGG TAGCGATGAA GGAAGGGAGC AGCAAAGTGA AGCCATTGAG TCGCTTAAAT 
AATGCTGATA AAGATATAGT TATTGTTTAT CTGCCTTATG GAGACAATGG AGGAGAAGCC 
ATTCTTTCTC AGGTGCATTT GGAACTGGAT ACCAATTCTG TGGATATCCA AACTGAAGAG 
GATTTTAATT TGCTTAAGAT GAGCAATCCC AAGAGATATG AAGTTCTCAA GGAGATTCTG 
ATATCACTTG GGCTGAGTTG TGAATTAAGT GAGATTCCTA TGTTGACGCC TATTTACCTT 
CTCTCTTCTG ATGAGGAAAT CCATCTTACG TTTTTGAAAT GGCTTGAGGA GAATGTAAAT 
GTTGAAGGAT TAATAGAATC CAGCAAAGTG TCTCTTAAAT TTGTTTCATC TTGTGAATCG 
AAAATGGAGA TAACTCCATC TCTCATGTCA GTCATCACCA AGATGGGAAA CTTCTCATCA 
GAACATTTCA GCCTGGAGAC ATATCAGCAG AATCTACAAA CAAAGAAACT TGGAAAGATT 
CTTTTCTTTA CTGAAGTTAC CACCACAACA ATGAATCTTC TAGACGGGTT AATGTTCAAG 
TTGCCTGAGG AGATGGGTTT AATAGCAATT GCTGTTCGAC AAACACAAGG GAAAGGTCGT 
GGCGGAAATG TTTGGCTCAG TCCCATTGGT TGTGCACTAT CCACTTTACA TATCACAATC 
CCTTTACATT CCAACTTGGG CCAGAGAATT CCTTTTATTC AACACCTAGT GTCCTTGGCA 
GTGGTAGAGT CGGTTAGATC AATACCAGGA TATGAGGATA TTGACCTGCG AGTAAAATGG 
CCAAATGATA TTTATTACAG TGATCTTATG AAGCTTGGTG GAGTTCTGGT AACCTCTACA 
CTTATTGAGA CAACATTTCA TATACTTATT GGCTTTGGAT TTAATGTGAA TAACAGCAAC 
CCCACCATAT GCATCAATGA CCTCATCACA AAATTTAATA AGGAAGAGGG GACAAATCTG 
AAGGCTTTAA CTGCAGACTG CCTTATTGCG AGAACTGTAA CTGTGCTAGA GAGGCTTATA 
GATATTTTTC AGGAGAAAGG GCCCAATGGA GTTCTTCCTC GTTACTACAA GTACTGGGTA 
CACAGTGGTA AGCAGGTTCG TCTCCACAAT GAGGAGGGCC CGTTGGCATG GATAGTTGGG 
ATTGATGATT ATGGATTCCT TCAAGTTCAT GAAGAAGGCA AAGGTGTAGA GTCTGTGCAT 
CCAGATGGCA ACTCTTTTGA CATGCTGAAA AACCTCATAA TCCCAAAGCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001003.1
CDS	256..2709
Translation	MQLLDDLNRWSLLLISPFIYPEKLLETEHIAFVTESVSAQADNLQKLPVSSKEIVKWSDYCSPLAYKPGEPYKLIAEASVDNFSNLGIAFMEDRLQMDNGMVPCKIVSVHLQETTLKELKQVTPSMKEKSVSITQMNEVTPDTFGTTVKTQLGVSESAGQGDSEAKEEKSKLVKENGDESNSLSDKETGFGEHHHLHLSSCRECLQLENSTIESVRFASAEDIPELPDDCNSKLEENGDCLTTGIKRVNLAGKPPNILIYVGSGTAKVDFEQVKSIIQECVDTDSYTIYQLHEEQVLKAPWIDNSLLLIIATEGPISEKNQKQFMKFLSKGGKILGLSSSFTFDGIQIKRKDKLKRTVHELVVSKMDSTEMKLNLLISGCIFEVAMKEGSSKVKPLSRLNNADKDIVIVYLPYGDNGGEAILSQVHLELDTNSVDIQTEEDFNLLKMSNPKRYEVLKEILISLGLSCELSEIPMLTPIYLLSSDEEIHLTFLKWLEENVNVEGLIESSKVSLKFVSSCESKMEITPSLMSVITKMGNFSSEHFSLETYQQNLQTKKLGKILFFTEVTTTTMNLLDGLMFKLPEEMGLIAIAVRQTQGKGRGGNVWLSPIGCALSTLHITIPLHSNLGQRIPFIQHLVSLAVVESVRSIPGYEDIDLRVKWPNDIYYSDLMKLGGVLVTSTLIETTFHILIGFGFNVNNSNPTICINDLITKFNKEEGTNLKALTADCLIARTVTVLERLIDIFQEKGPNGVLPRYYKYWVHSGKQVRLHNEEGPLAWIVGIDDYGFLQVHEEGKGVESVHPDGNSFDMLKNLIIPKH

Target ORF information:

RefSeq Version	XM_025145235.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145235.1

ORF Insert Sequence:

ATGCAACTCC TCGATGATTT AAACAGATGG AGTCTACTTC TCATTTCTCC TTTTATTTAT 
CCTGAAAAGT TACTAGAAAC AGAACATATA GCCTTTGTGA CAGAAAGTGT TTCAGCTCAG 
GCAGATAACT TGCAGAAATT ACCTGTCTCT TCAAAAGAGA TTGTGAAGTG GTCTGACTAT 
TGTTCACCGT TGGCCTATAA ACCTGGGGAA CCTTATAAGT TAATTGCTGA AGCAAGCGTA 
GATAATTTCA GTAACCTTGG AATAGCATTC ATGGAAGACA GGCTTCAAAT GGATAACGGA 
ATGGTTCCAT GCAAGATTGT TTCTGTCCAT TTACAAGAAA CTACTCTGAA GGAGTTAAAA 
CAGGTGACAC CATCTATGAA AGAGAAGAGT GTAAGCATTA CACAGATGAA TGAAGTAACT 
CCTGATACTT TTGGGACTAC TGTTAAAACA CAATTAGGAG TATCTGAGTC TGCAGGACAG 
GGTGATTCAG AGGCAAAAGA AGAGAAATCT AAGCTAGTGA AAGAAAATGG AGATGAATCC 
AACAGCCTAT CTGACAAAGA GACAGGTTTT GGAGAACATC ATCACTTGCA TCTTTCCAGC 
TGTCGTGAAT GTCTGCAACT TGAAAACAGC ACTATTGAAT CAGTCAGATT TGCCTCTGCG 
GAAGACATTC CAGAACTTCC TGATGATTGC AATAGCAAAC TGGAAGAGAA TGGTGATTGT 
CTGACTACAG GAATAAAGAG AGTAAACCTT GCTGGAAAGC CTCCTAATAT ATTGATTTAT 
GTTGGCTCTG GAACTGCAAA AGTGGACTTT GAGCAAGTAA AATCAATCAT TCAGGAATGT 
GTTGATACAG ACAGCTATAC CATCTATCAG CTGCATGAGG AACAAGTTCT GAAAGCTCCG 
TGGATTGATA ATTCACTGCT GTTGATCATT GCCACAGAGG GGCCTATTTC TGAGAAGAAC 
CAAAAACAGT TTATGAAGTT TTTATCTAAA GGTGGAAAGA TTCTAGGGTT ATCTTCCTCT 
TTTACATTTG ATGGTATACA AATAAAGAGG AAAGACAAAC TGAAGAGAAC TGTTCATGAA 
TTAGTGGTCT CTAAAATGGA CAGTACTGAA ATGAAGTTAA ATCTTTTGAT CAGTGGCTGT 
ATTTTTGAGG TAGCGATGAA GGAAGGGAGC AGCAAAGTGA AGCCATTGAG TCGCTTAAAT 
AATGCTGATA AAGATATAGT TATTGTTTAT CTGCCTTATG GAGACAATGG AGGAGAAGCC 
ATTCTTTCTC AGGTGCATTT GGAACTGGAT ACCAATTCTG TGGATATCCA AACTGAAGAG 
GATTTTAATT TGCTTAAGAT GAGCAATCCC AAGAGATATG AAGTTCTCAA GGAGATTCTG 
ATATCACTTG GGCTGAGTTG TGAATTAAGT GAGATTCCTA TGTTGACGCC TATTTACCTT 
CTCTCTTCTG ATGAGGAAAT CCATCTTACG TTTTTGAAAT GGCTTGAGGA GAATGTAAAT 
GTTGAAGGAT TAATAGAATC CAGCAAAGTG TCTCTTAAAT TTGTTTCATC TTGTGAATCG 
AAAATGGAGA TAACTCCATC TCTCATGTCA GTCATCACCA AGATGGGAAA CTTCTCATCA 
GAACATTTCA GCCTGGAGAC ATATCAGCAG AATCTACAAA CAAAGAAACT TGGAAAGATT 
CTTTTCTTTA CTGAAGTTAC CACCACAACA ATGAATCTTC TAGACGGGTT AATGTTCAAG 
TTGCCTGAGG AGATGGGTTT AATAGCAATT GCTGTTCGAC AAACACAAGG GAAAGGTCGT 
GGCGGAAATG TTTGGCTCAG TCCCATTGGT TGTGCACTAT CCACTTTACA TATCACAATC 
CCTTTACATT CCAACTTGGG CCAGAGAATT CCTTTTATTC AACACCTAGT GTCCTTGGCA 
GTGGTAGAGT CGGTTAGATC AATACCAGGA TATGAGGATA TTGACCTGCG AGTAAAATGG 
CCAAATGATA TTTATTACAG TGATCTTATG AAGCTTGGTG GAGTTCTGGT AACCTCTACA 
CTTATTGAGA CAACATTTCA TATACTTATT GGCTTTGGAT TTAATGTGAA TAACAGCAAC 
CCCACCATAT GCATCAATGA CCTCATCACA AAATTTAATA AGGAAGAGGG GACAAATCTG 
AAGGCTTTAA CTGCAGACTG CCTTATTGCG AGAACTGTAA CTGTGCTAGA GAGGCTTATA 
GATATTTTTC AGGAGAAAGG GCCCAATGGA GTTCTTCCTC GTTACTACAA GTACTGGGTA 
CACAGTGGTA AGCAGGTTCG TCTCCACAAT GAGGAGGGCC CGTTGGCATG GATAGTTGGG 
ATTGATGATT ATGGATTCCT TCAAGTTCAT GAAGAAGGCA AAGGTGTAGA GTCTGTGCAT 
CCAGATGGCA ACTCTTTTGA CATGCTGAAA AACCTCATAA TCCCAAAGCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_025145233.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001001.1 Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa142939
Clone ID Related Accession (Same CDS sequence)	XM_025145233.1
Accession Version	XM_025145233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATCA CGCTCTGCTA CCTCTACCTA TGGGCGCGCT GGGGGCCTTG CTCGGCCGCG 
CTCATCCGCC GCACCGTGCG CCGCCTCCAC CGCTCCCGTT GCTCCTTCAT CTTCTGCTGC 
TCCGCCGGCC CTTCGGCGCC TCCGGCTCAT CCGCCGCGCA GGCCGGCGCC TCCCGAGGAG 
CGCGTCTGCC TCAGGATAGG AAATAAAGTC TTCTTCACGG ACGAGACGCA GATTGTGAAG 
TGGTCTGACT ATTGTTCACC GTTGGCCTAT AAACCTGGGG AACCTTATAA GTTAATTGCT 
GAAGCAAGCG TAGATAATTT CAGTAACCTT GGAATAGCAT TCATGGAAGA CAGGCTTCAA 
ATGGATAACG GAATGGTTCC ATGCAAGATT GTTTCTGTCC ATTTACAAGA AACTACTCTG 
AAGGAGTTAA AACAGGTGAC ACCATCTATG AAAGAGAAGA GTGTAAGCAT TACACAGATG 
AATGAAGTAA CTCCTGATAC TTTTGGGACT ACTGTTAAAA CACAATTAGG AGTATCTGAG 
TCTGCAGGAC AGGGTGATTC AGAGGCAAAA GAAGAGAAAT CTAAGCTAGT GAAAGAAAAT 
GGAGATGAAT CCAACAGCCT ATCTGACAAA GAGACAGGTT TTGGAGAACA TCATCACTTG 
CATCTTTCCA GCTGTCGTGA ATGTCTGCAA CTTGAAAACA GCACTATTGA ATCAGTCAGA 
TTTGCCTCTG CGGAAGACAT TCCAGAACTT CCTGATGATT GCAATAGCAA ACTGGAAGAG 
AATGGTGATT GTCTGACTAC AGGAATAAAG AGAGTAAACC TTGCTGGAAA GCCTCCTAAT 
ATATTGATTT ATGTTGGCTC TGGAACTGCA AAAGTGGACT TTGAGCAAGT AAAATCAATC 
ATTCAGGAAT GTGTTGATAC AGACAGCTAT ACCATCTATC AGCTGCATGA GGAACAAGTT 
CTGAAAGCTC CGTGGATTGA TAATTCACTG CTGTTGATCA TTGCCACAGA GGGGCCTATT 
TCTGAGAAGA ACCAAAAACA GTTTATGAAG TTTTTATCTA AAGGTGGAAA GATTCTAGGG 
TTATCTTCCT CTTTTACATT TGATGGTATA CAAATAAAGA GGAAAGACAA ACTGAAGAGA 
ACTGTTCATG AATTAGTGGT CTCTAAAATG GACAGTACTG AAATGAAGTT AAATCTTTTG 
ATCAGTGGCT GTATTTTTGA GGTAGCGATG AAGGAAGGGA GCAGCAAAGT GAAGCCATTG 
AGTCGCTTAA ATAATGCTGA TAAAGATATA GTTATTGTTT ATCTGCCTTA TGGAGACAAT 
GGAGGAGAAG CCATTCTTTC TCAGGTGCAT TTGGAACTGG ATACCAATTC TGTGGATATC 
CAAACTGAAG AGGATTTTAA TTTGCTTAAG ATGAGCAATC CCAAGAGATA TGAAGTTCTC 
AAGGAGATTC TGATATCACT TGGGCTGAGT TGTGAATTAA GTGAGATTCC TATGTTGACG 
CCTATTTACC TTCTCTCTTC TGATGAGGAA ATCCATCTTA CGTTTTTGAA ATGGCTTGAG 
GAGAATGTAA ATGTTGAAGG ATTAATAGAA TCCAGCAAAG TGTCTCTTAA ATTTGTTTCA 
TCTTGTGAAT CGAAAATGGA GATAACTCCA TCTCTCATGT CAGTCATCAC CAAGATGGGA 
AACTTCTCAT CAGAACATTT CAGCCTGGAG ACATATCAGC AGAATCTACA AACAAAGAAA 
CTTGGAAAGA TTCTTTTCTT TACTGAAGTT ACCACCACAA CAATGAATCT TCTAGACGGG 
TTAATGTTCA AGTTGCCTGA GGAGATGGGT TTAATAGCAA TTGCTGTTCG ACAAACACAA 
GGGAAAGGTC GTGGCGGAAA TGTTTGGCTC AGTCCCATTG GTTGTGCACT ATCCACTTTA 
CATATCACAA TCCCTTTACA TTCCAACTTG GGCCAGAGAA TTCCTTTTAT TCAACACCTA 
GTGTCCTTGG CAGTGGTAGA GTCGGTTAGA TCAATACCAG GATATGAGGA TATTGACCTG 
CGAGTAAAAT GGCCAAATGA TATTTATTAC AGTGATCTTA TGAAGCTTGG TGGAGTTCTG 
GTAACCTCTA CACTTATTGA GACAACATTT CATATACTTA TTGGCTTTGG ATTTAATGTG 
AATAACAGCA ACCCCACCAT ATGCATCAAT GACCTCATCA CAAAATTTAA TAAGGAAGAG 
GGGACAAATC TGAAGGCTTT AACTGCAGAC TGCCTTATTG CGAGAACTGT AACTGTGCTA 
GAGAGGCTTA TAGATATTTT TCAGGAGAAA GGGCCCAATG GAGTTCTTCC TCGTTACTAC 
AAGTACTGGG TACACAGTGG TAAGCAGGTT CGTCTCCACA ATGAGGAGGG CCCGTTGGCA 
TGGATAGTTG GGATTGATGA TTATGGATTC CTTCAAGTTC ATGAAGAAGG CAAAGGTGTA 
GAGTCTGTGC ATCCAGATGG CAACTCTTTT GACATGCTGA AAAACCTCAT AATCCCAAAG 
CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001001.1
CDS	40..2565
Translation	MLITLCYLYLWARWGPCSAALIRRTVRRLHRSRCSFIFCCSAGPSAPPAHPPRRPAPPEERVCLRIGNKVFFTDETQIVKWSDYCSPLAYKPGEPYKLIAEASVDNFSNLGIAFMEDRLQMDNGMVPCKIVSVHLQETTLKELKQVTPSMKEKSVSITQMNEVTPDTFGTTVKTQLGVSESAGQGDSEAKEEKSKLVKENGDESNSLSDKETGFGEHHHLHLSSCRECLQLENSTIESVRFASAEDIPELPDDCNSKLEENGDCLTTGIKRVNLAGKPPNILIYVGSGTAKVDFEQVKSIIQECVDTDSYTIYQLHEEQVLKAPWIDNSLLLIIATEGPISEKNQKQFMKFLSKGGKILGLSSSFTFDGIQIKRKDKLKRTVHELVVSKMDSTEMKLNLLISGCIFEVAMKEGSSKVKPLSRLNNADKDIVIVYLPYGDNGGEAILSQVHLELDTNSVDIQTEEDFNLLKMSNPKRYEVLKEILISLGLSCELSEIPMLTPIYLLSSDEEIHLTFLKWLEENVNVEGLIESSKVSLKFVSSCESKMEITPSLMSVITKMGNFSSEHFSLETYQQNLQTKKLGKILFFTEVTTTTMNLLDGLMFKLPEEMGLIAIAVRQTQGKGRGGNVWLSPIGCALSTLHITIPLHSNLGQRIPFIQHLVSLAVVESVRSIPGYEDIDLRVKWPNDIYYSDLMKLGGVLVTSTLIETTFHILIGFGFNVNNSNPTICINDLITKFNKEEGTNLKALTADCLIARTVTVLERLIDIFQEKGPNGVLPRYYKYWVHSGKQVRLHNEEGPLAWIVGIDDYGFLQVHEEGKGVESVHPDGNSFDMLKNLIIPKH

Target ORF information:

RefSeq Version	XM_025145233.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145233.1

ORF Insert Sequence:

ATGCTGATCA CGCTCTGCTA CCTCTACCTA TGGGCGCGCT GGGGGCCTTG CTCGGCCGCG 
CTCATCCGCC GCACCGTGCG CCGCCTCCAC CGCTCCCGTT GCTCCTTCAT CTTCTGCTGC 
TCCGCCGGCC CTTCGGCGCC TCCGGCTCAT CCGCCGCGCA GGCCGGCGCC TCCCGAGGAG 
CGCGTCTGCC TCAGGATAGG AAATAAAGTC TTCTTCACGG ACGAGACGCA GATTGTGAAG 
TGGTCTGACT ATTGTTCACC GTTGGCCTAT AAACCTGGGG AACCTTATAA GTTAATTGCT 
GAAGCAAGCG TAGATAATTT CAGTAACCTT GGAATAGCAT TCATGGAAGA CAGGCTTCAA 
ATGGATAACG GAATGGTTCC ATGCAAGATT GTTTCTGTCC ATTTACAAGA AACTACTCTG 
AAGGAGTTAA AACAGGTGAC ACCATCTATG AAAGAGAAGA GTGTAAGCAT TACACAGATG 
AATGAAGTAA CTCCTGATAC TTTTGGGACT ACTGTTAAAA CACAATTAGG AGTATCTGAG 
TCTGCAGGAC AGGGTGATTC AGAGGCAAAA GAAGAGAAAT CTAAGCTAGT GAAAGAAAAT 
GGAGATGAAT CCAACAGCCT ATCTGACAAA GAGACAGGTT TTGGAGAACA TCATCACTTG 
CATCTTTCCA GCTGTCGTGA ATGTCTGCAA CTTGAAAACA GCACTATTGA ATCAGTCAGA 
TTTGCCTCTG CGGAAGACAT TCCAGAACTT CCTGATGATT GCAATAGCAA ACTGGAAGAG 
AATGGTGATT GTCTGACTAC AGGAATAAAG AGAGTAAACC TTGCTGGAAA GCCTCCTAAT 
ATATTGATTT ATGTTGGCTC TGGAACTGCA AAAGTGGACT TTGAGCAAGT AAAATCAATC 
ATTCAGGAAT GTGTTGATAC AGACAGCTAT ACCATCTATC AGCTGCATGA GGAACAAGTT 
CTGAAAGCTC CGTGGATTGA TAATTCACTG CTGTTGATCA TTGCCACAGA GGGGCCTATT 
TCTGAGAAGA ACCAAAAACA GTTTATGAAG TTTTTATCTA AAGGTGGAAA GATTCTAGGG 
TTATCTTCCT CTTTTACATT TGATGGTATA CAAATAAAGA GGAAAGACAA ACTGAAGAGA 
ACTGTTCATG AATTAGTGGT CTCTAAAATG GACAGTACTG AAATGAAGTT AAATCTTTTG 
ATCAGTGGCT GTATTTTTGA GGTAGCGATG AAGGAAGGGA GCAGCAAAGT GAAGCCATTG 
AGTCGCTTAA ATAATGCTGA TAAAGATATA GTTATTGTTT ATCTGCCTTA TGGAGACAAT 
GGAGGAGAAG CCATTCTTTC TCAGGTGCAT TTGGAACTGG ATACCAATTC TGTGGATATC 
CAAACTGAAG AGGATTTTAA TTTGCTTAAG ATGAGCAATC CCAAGAGATA TGAAGTTCTC 
AAGGAGATTC TGATATCACT TGGGCTGAGT TGTGAATTAA GTGAGATTCC TATGTTGACG 
CCTATTTACC TTCTCTCTTC TGATGAGGAA ATCCATCTTA CGTTTTTGAA ATGGCTTGAG 
GAGAATGTAA ATGTTGAAGG ATTAATAGAA TCCAGCAAAG TGTCTCTTAA ATTTGTTTCA 
TCTTGTGAAT CGAAAATGGA GATAACTCCA TCTCTCATGT CAGTCATCAC CAAGATGGGA 
AACTTCTCAT CAGAACATTT CAGCCTGGAG ACATATCAGC AGAATCTACA AACAAAGAAA 
CTTGGAAAGA TTCTTTTCTT TACTGAAGTT ACCACCACAA CAATGAATCT TCTAGACGGG 
TTAATGTTCA AGTTGCCTGA GGAGATGGGT TTAATAGCAA TTGCTGTTCG ACAAACACAA 
GGGAAAGGTC GTGGCGGAAA TGTTTGGCTC AGTCCCATTG GTTGTGCACT ATCCACTTTA 
CATATCACAA TCCCTTTACA TTCCAACTTG GGCCAGAGAA TTCCTTTTAT TCAACACCTA 
GTGTCCTTGG CAGTGGTAGA GTCGGTTAGA TCAATACCAG GATATGAGGA TATTGACCTG 
CGAGTAAAAT GGCCAAATGA TATTTATTAC AGTGATCTTA TGAAGCTTGG TGGAGTTCTG 
GTAACCTCTA CACTTATTGA GACAACATTT CATATACTTA TTGGCTTTGG ATTTAATGTG 
AATAACAGCA ACCCCACCAT ATGCATCAAT GACCTCATCA CAAAATTTAA TAAGGAAGAG 
GGGACAAATC TGAAGGCTTT AACTGCAGAC TGCCTTATTG CGAGAACTGT AACTGTGCTA 
GAGAGGCTTA TAGATATTTT TCAGGAGAAA GGGCCCAATG GAGTTCTTCC TCGTTACTAC 
AAGTACTGGG TACACAGTGG TAAGCAGGTT CGTCTCCACA ATGAGGAGGG CCCGTTGGCA 
TGGATAGTTG GGATTGATGA TTATGGATTC CTTCAAGTTC ATGAAGAAGG CAAAGGTGTA 
GAGTCTGTGC ATCCAGATGG CAACTCTTTT GACATGCTGA AAAACCTCAT AATCCCAAAG 
CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_015301496.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156982.1 Gallus gallus holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa30361
Clone ID Related Accession (Same CDS sequence)	XM_015301496.1 , XM_015301496.2 , XM_025145239.1
Accession Version	XM_015301496.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455738.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA GGCTTCAAAT GGATAACGGA ATGGTTCCAT GCAAGATTGT TTCTGTCCAT 
TTACAAGAAA CTACTCTGAA GGAGTTAAAA CAGGTGACAC CATCTATGAA AGAGAAGAGT 
GTAAGCATTA CACAGATGAA TGAAGTAACT CCTGATACTT TTGGGACTAC TGTTAAAACA 
CAATTAGGAG TATCTGAGTC TGCAGGACAG GGTGATTCAG AGGCAAAAGA AGAGAAATCT 
AAGCTAGTGA AAGAAAATGG AGATGAATCC AACAGCCTAT CTGACAAAGA GACAGGTTTT 
GGAGAACATC ATCACTTGCA TCTTTCCAGC TGTCGTGAAT GTCTGCAACT TGAAAACAGC 
ACTATTGAAT CAGTCAGATT TGCCTCTGCG GAAGACATTC CAGAACTTCC TGATGATTGC 
AATAGCAAAC TGGAAGAGAA TGGTGATTGT CTGACTACAG GAATAAAGAG AGTAAACCTT 
GCTGGAAAGC CTCCTAATAT ATTGATTTAT GTTGGCTCTG GAACTGCAAA AGTGGACTTT 
GAGCAAGTAA AATCAATCAT TCAGGAATGT GTTGATACAG ACAGCTATAC CATCTATCAG 
CTGCATGAGG AACAAGTTCT GAAAGCTCCG TGGATTGATA ATTCACTGCT GTTGATCATT 
GCCACAGAGG GGCCTATTTC TGAGAAGAAC CAAAAACAGT TTATGAAGTT TTTATCTAAA 
GGTGGAAAGA TTCTAGGGTT ATCTTCCTCT TTTACATTTG ATGGTATACA AATAAAGAGG 
AAAGACAAAC TGAAGAGAAC TGTTCATGAA TTAGTGGTCT CTAAAATGGA CAGTACTGAA 
ATGAAGTTAA ATCTTTTGAT CAGTGGCTGT ATTTTTGAGG TAGCGATGAA GGAAGGGAGC 
AGCAAAGTGA AGCCATTGAG TCGCTTAAAT AATGCTGATA AAGATATAGT TATTGTTTAT 
CTGCCTTATG GAGACAATGG AGGAGAAGCC ATTCTTTCTC AGGTGCATTT GGAACTGGAT 
ACCAATTCTG TGGATATCCA AACTGAAGAG GATTTTAATT TGCTTAAGAT GAGCAATCCC 
AAGAGATATG AAGTTCTCAA GGAGATTCTG ATATCACTTG GGCTGAGTTG TGAATTAAGT 
GAGATTCCTA TGTTGACGCC TATTTACCTT CTCTCTTCTG ATGAGGAAAT CCATCTTACG 
TTTTTGAAAT GGCTTGAGGA GAATGTAAAT GTTGAAGGAT TAATAGAATC CAGCAAAGTG 
TCTCTTAAAT TTGTTTCATC TTGTGAATCG AAAATGGAGA TAACTCCATC TCTCATGTCA 
GTCATCACCA AGATGGGAAA CTTCTCATCA GAACATTTCA GCCTGGAGAC ATATCAGCAG 
AATCTACAAA CAAAGAAACT TGGAAAGATT CTTTTCTTTA CTGAAGTTAC CACCACAACA 
ATGAATCTTC TAGACGGGTT AATGTTCAAG TTGCCTGAGG AGATGGGTTT AATAGCAATT 
GCTGTTCGAC AAACACAAGG GAAAGGTCGT GGCGGAAATG TTTGGCTCAG TCCCATTGGT 
TGTGCACTAT CCACTTTACA TATCACAATC CCTTTACATT CCAACTTGGG CCAGAGAATT 
CCTTTTATTC AACACCTAGT GTCCTTGGCA GTGGTAGAGT CGGTTAGATC AATACCAGGA 
TATGAGGATA TTGACCTGCG AGTAAAATGG CCAAATGATA TTTATTACAG TGATCTTATG 
AAGCTTGGTG GAGTTCTGGT AACCTCTACA CTTATTGAGA CAACATTTCA TATACTTATT 
GGCTTTGGAT TTAATGTGAA TAACAGCAAC CCCACCATAT GCATCAATGA CCTCATCACA 
AAATTTAATA AGGAAGAGGG GACAAATCTG AAGGCTTTAA CTGCAGACTG CCTTATTGCG 
AGAACTGTAA CTGTGCTAGA GAGGCTTATA GATATTTTTC AGGAGAAAGG GCCCAATGGA 
GTTCTTCCTC GTTACTACAA GTACTGGGTA CACAGTGGTA AGCAGGTTCG TCTCCACAAT 
GAGGAGGGCC CGTTGGCATG GATAGTTGGG ATTGATGATT ATGGATTCCT TCAAGTTCAT 
GAAGAAGGCA AAGGTGTAGA GTCTGTGCAT CCAGATGGCA ACTCTTTTGA CATGCTGAAA 
AACCTCATAA TCCCAAAGCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156982.1
CDS	228..2411
Translation	MEDRLQMDNGMVPCKIVSVHLQETTLKELKQVTPSMKEKSVSITQMNEVTPDTFGTTVKTQLGVSESAGQGDSEAKEEKSKLVKENGDESNSLSDKETGFGEHHHLHLSSCRECLQLENSTIESVRFASAEDIPELPDDCNSKLEENGDCLTTGIKRVNLAGKPPNILIYVGSGTAKVDFEQVKSIIQECVDTDSYTIYQLHEEQVLKAPWIDNSLLLIIATEGPISEKNQKQFMKFLSKGGKILGLSSSFTFDGIQIKRKDKLKRTVHELVVSKMDSTEMKLNLLISGCIFEVAMKEGSSKVKPLSRLNNADKDIVIVYLPYGDNGGEAILSQVHLELDTNSVDIQTEEDFNLLKMSNPKRYEVLKEILISLGLSCELSEIPMLTPIYLLSSDEEIHLTFLKWLEENVNVEGLIESSKVSLKFVSSCESKMEITPSLMSVITKMGNFSSEHFSLETYQQNLQTKKLGKILFFTEVTTTTMNLLDGLMFKLPEEMGLIAIAVRQTQGKGRGGNVWLSPIGCALSTLHITIPLHSNLGQRIPFIQHLVSLAVVESVRSIPGYEDIDLRVKWPNDIYYSDLMKLGGVLVTSTLIETTFHILIGFGFNVNNSNPTICINDLITKFNKEEGTNLKALTADCLIARTVTVLERLIDIFQEKGPNGVLPRYYKYWVHSGKQVRLHNEEGPLAWIVGIDDYGFLQVHEEGKGVESVHPDGNSFDMLKNLIIPKH

Target ORF information:

RefSeq Version	XM_015301496.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301496.1

ORF Insert Sequence:

ATGGAAGACA GGCTTCAAAT GGATAACGGA ATGGTTCCAT GCAAGATTGT TTCTGTCCAT 
TTACAAGAAA CTACTCTGAA GGAGTTAAAA CAGGTGACAC CATCTATGAA AGAGAAGAGT 
GTAAGCATTA CACAGATGAA TGAAGTAACT CCTGATACTT TTGGGACTAC TGTTAAAACA 
CAATTAGGAG TATCTGAGTC TGCAGGACAG GGTGATTCAG AGGCAAAAGA AGAGAAATCT 
AAGCTAGTGA AAGAAAATGG AGATGAATCC AACAGCCTAT CTGACAAAGA GACAGGTTTT 
GGAGAACATC ATCACTTGCA TCTTTCCAGC TGTCGTGAAT GTCTGCAACT TGAAAACAGC 
ACTATTGAAT CAGTCAGATT TGCCTCTGCG GAAGACATTC CAGAACTTCC TGATGATTGC 
AATAGCAAAC TGGAAGAGAA TGGTGATTGT CTGACTACAG GAATAAAGAG AGTAAACCTT 
GCTGGAAAGC CTCCTAATAT ATTGATTTAT GTTGGCTCTG GAACTGCAAA AGTGGACTTT 
GAGCAAGTAA AATCAATCAT TCAGGAATGT GTTGATACAG ACAGCTATAC CATCTATCAG 
CTGCATGAGG AACAAGTTCT GAAAGCTCCG TGGATTGATA ATTCACTGCT GTTGATCATT 
GCCACAGAGG GGCCTATTTC TGAGAAGAAC CAAAAACAGT TTATGAAGTT TTTATCTAAA 
GGTGGAAAGA TTCTAGGGTT ATCTTCCTCT TTTACATTTG ATGGTATACA AATAAAGAGG 
AAAGACAAAC TGAAGAGAAC TGTTCATGAA TTAGTGGTCT CTAAAATGGA CAGTACTGAA 
ATGAAGTTAA ATCTTTTGAT CAGTGGCTGT ATTTTTGAGG TAGCGATGAA GGAAGGGAGC 
AGCAAAGTGA AGCCATTGAG TCGCTTAAAT AATGCTGATA AAGATATAGT TATTGTTTAT 
CTGCCTTATG GAGACAATGG AGGAGAAGCC ATTCTTTCTC AGGTGCATTT GGAACTGGAT 
ACCAATTCTG TGGATATCCA AACTGAAGAG GATTTTAATT TGCTTAAGAT GAGCAATCCC 
AAGAGATATG AAGTTCTCAA GGAGATTCTG ATATCACTTG GGCTGAGTTG TGAATTAAGT 
GAGATTCCTA TGTTGACGCC TATTTACCTT CTCTCTTCTG ATGAGGAAAT CCATCTTACG 
TTTTTGAAAT GGCTTGAGGA GAATGTAAAT GTTGAAGGAT TAATAGAATC CAGCAAAGTG 
TCTCTTAAAT TTGTTTCATC TTGTGAATCG AAAATGGAGA TAACTCCATC TCTCATGTCA 
GTCATCACCA AGATGGGAAA CTTCTCATCA GAACATTTCA GCCTGGAGAC ATATCAGCAG 
AATCTACAAA CAAAGAAACT TGGAAAGATT CTTTTCTTTA CTGAAGTTAC CACCACAACA 
ATGAATCTTC TAGACGGGTT AATGTTCAAG TTGCCTGAGG AGATGGGTTT AATAGCAATT 
GCTGTTCGAC AAACACAAGG GAAAGGTCGT GGCGGAAATG TTTGGCTCAG TCCCATTGGT 
TGTGCACTAT CCACTTTACA TATCACAATC CCTTTACATT CCAACTTGGG CCAGAGAATT 
CCTTTTATTC AACACCTAGT GTCCTTGGCA GTGGTAGAGT CGGTTAGATC AATACCAGGA 
TATGAGGATA TTGACCTGCG AGTAAAATGG CCAAATGATA TTTATTACAG TGATCTTATG 
AAGCTTGGTG GAGTTCTGGT AACCTCTACA CTTATTGAGA CAACATTTCA TATACTTATT 
GGCTTTGGAT TTAATGTGAA TAACAGCAAC CCCACCATAT GCATCAATGA CCTCATCACA 
AAATTTAATA AGGAAGAGGG GACAAATCTG AAGGCTTTAA CTGCAGACTG CCTTATTGCG 
AGAACTGTAA CTGTGCTAGA GAGGCTTATA GATATTTTTC AGGAGAAAGG GCCCAATGGA 
GTTCTTCCTC GTTACTACAA GTACTGGGTA CACAGTGGTA AGCAGGTTCG TCTCCACAAT 
GAGGAGGGCC CGTTGGCATG GATAGTTGGG ATTGATGATT ATGGATTCCT TCAAGTTCAT 
GAAGAAGGCA AAGGTGTAGA GTCTGTGCAT CCAGATGGCA ACTCTTTTGA CATGCTGAAA 
AACCTCATAA TCCCAAAGCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_015301496.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156982.1 Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa30361
Clone ID Related Accession (Same CDS sequence)	XM_015301496.1 , XM_015301496.2 , XM_025145239.1
Accession Version	XM_015301496.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	biotin--protein ligase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015301496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA GGCTTCAAAT GGATAACGGA ATGGTTCCAT GCAAGATTGT TTCTGTCCAT 
TTACAAGAAA CTACTCTGAA GGAGTTAAAA CAGGTGACAC CATCTATGAA AGAGAAGAGT 
GTAAGCATTA CACAGATGAA TGAAGTAACT CCTGATACTT TTGGGACTAC TGTTAAAACA 
CAATTAGGAG TATCTGAGTC TGCAGGACAG GGTGATTCAG AGGCAAAAGA AGAGAAATCT 
AAGCTAGTGA AAGAAAATGG AGATGAATCC AACAGCCTAT CTGACAAAGA GACAGGTTTT 
GGAGAACATC ATCACTTGCA TCTTTCCAGC TGTCGTGAAT GTCTGCAACT TGAAAACAGC 
ACTATTGAAT CAGTCAGATT TGCCTCTGCG GAAGACATTC CAGAACTTCC TGATGATTGC 
AATAGCAAAC TGGAAGAGAA TGGTGATTGT CTGACTACAG GAATAAAGAG AGTAAACCTT 
GCTGGAAAGC CTCCTAATAT ATTGATTTAT GTTGGCTCTG GAACTGCAAA AGTGGACTTT 
GAGCAAGTAA AATCAATCAT TCAGGAATGT GTTGATACAG ACAGCTATAC CATCTATCAG 
CTGCATGAGG AACAAGTTCT GAAAGCTCCG TGGATTGATA ATTCACTGCT GTTGATCATT 
GCCACAGAGG GGCCTATTTC TGAGAAGAAC CAAAAACAGT TTATGAAGTT TTTATCTAAA 
GGTGGAAAGA TTCTAGGGTT ATCTTCCTCT TTTACATTTG ATGGTATACA AATAAAGAGG 
AAAGACAAAC TGAAGAGAAC TGTTCATGAA TTAGTGGTCT CTAAAATGGA CAGTACTGAA 
ATGAAGTTAA ATCTTTTGAT CAGTGGCTGT ATTTTTGAGG TAGCGATGAA GGAAGGGAGC 
AGCAAAGTGA AGCCATTGAG TCGCTTAAAT AATGCTGATA AAGATATAGT TATTGTTTAT 
CTGCCTTATG GAGACAATGG AGGAGAAGCC ATTCTTTCTC AGGTGCATTT GGAACTGGAT 
ACCAATTCTG TGGATATCCA AACTGAAGAG GATTTTAATT TGCTTAAGAT GAGCAATCCC 
AAGAGATATG AAGTTCTCAA GGAGATTCTG ATATCACTTG GGCTGAGTTG TGAATTAAGT 
GAGATTCCTA TGTTGACGCC TATTTACCTT CTCTCTTCTG ATGAGGAAAT CCATCTTACG 
TTTTTGAAAT GGCTTGAGGA GAATGTAAAT GTTGAAGGAT TAATAGAATC CAGCAAAGTG 
TCTCTTAAAT TTGTTTCATC TTGTGAATCG AAAATGGAGA TAACTCCATC TCTCATGTCA 
GTCATCACCA AGATGGGAAA CTTCTCATCA GAACATTTCA GCCTGGAGAC ATATCAGCAG 
AATCTACAAA CAAAGAAACT TGGAAAGATT CTTTTCTTTA CTGAAGTTAC CACCACAACA 
ATGAATCTTC TAGACGGGTT AATGTTCAAG TTGCCTGAGG AGATGGGTTT AATAGCAATT 
GCTGTTCGAC AAACACAAGG GAAAGGTCGT GGCGGAAATG TTTGGCTCAG TCCCATTGGT 
TGTGCACTAT CCACTTTACA TATCACAATC CCTTTACATT CCAACTTGGG CCAGAGAATT 
CCTTTTATTC AACACCTAGT GTCCTTGGCA GTGGTAGAGT CGGTTAGATC AATACCAGGA 
TATGAGGATA TTGACCTGCG AGTAAAATGG CCAAATGATA TTTATTACAG TGATCTTATG 
AAGCTTGGTG GAGTTCTGGT AACCTCTACA CTTATTGAGA CAACATTTCA TATACTTATT 
GGCTTTGGAT TTAATGTGAA TAACAGCAAC CCCACCATAT GCATCAATGA CCTCATCACA 
AAATTTAATA AGGAAGAGGG GACAAATCTG AAGGCTTTAA CTGCAGACTG CCTTATTGCG 
AGAACTGTAA CTGTGCTAGA GAGGCTTATA GATATTTTTC AGGAGAAAGG GCCCAATGGA 
GTTCTTCCTC GTTACTACAA GTACTGGGTA CACAGTGGTA AGCAGGTTCG TCTCCACAAT 
GAGGAGGGCC CGTTGGCATG GATAGTTGGG ATTGATGATT ATGGATTCCT TCAAGTTCAT 
GAAGAAGGCA AAGGTGTAGA GTCTGTGCAT CCAGATGGCA ACTCTTTTGA CATGCTGAAA 
AACCTCATAA TCCCAAAGCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156982.1
CDS	409..2592
Translation	MEDRLQMDNGMVPCKIVSVHLQETTLKELKQVTPSMKEKSVSITQMNEVTPDTFGTTVKTQLGVSESAGQGDSEAKEEKSKLVKENGDESNSLSDKETGFGEHHHLHLSSCRECLQLENSTIESVRFASAEDIPELPDDCNSKLEENGDCLTTGIKRVNLAGKPPNILIYVGSGTAKVDFEQVKSIIQECVDTDSYTIYQLHEEQVLKAPWIDNSLLLIIATEGPISEKNQKQFMKFLSKGGKILGLSSSFTFDGIQIKRKDKLKRTVHELVVSKMDSTEMKLNLLISGCIFEVAMKEGSSKVKPLSRLNNADKDIVIVYLPYGDNGGEAILSQVHLELDTNSVDIQTEEDFNLLKMSNPKRYEVLKEILISLGLSCELSEIPMLTPIYLLSSDEEIHLTFLKWLEENVNVEGLIESSKVSLKFVSSCESKMEITPSLMSVITKMGNFSSEHFSLETYQQNLQTKKLGKILFFTEVTTTTMNLLDGLMFKLPEEMGLIAIAVRQTQGKGRGGNVWLSPIGCALSTLHITIPLHSNLGQRIPFIQHLVSLAVVESVRSIPGYEDIDLRVKWPNDIYYSDLMKLGGVLVTSTLIETTFHILIGFGFNVNNSNPTICINDLITKFNKEEGTNLKALTADCLIARTVTVLERLIDIFQEKGPNGVLPRYYKYWVHSGKQVRLHNEEGPLAWIVGIDDYGFLQVHEEGKGVESVHPDGNSFDMLKNLIIPKH

Target ORF information:

RefSeq Version	XM_015301496.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301496.2

ORF Insert Sequence:

ATGGAAGACA GGCTTCAAAT GGATAACGGA ATGGTTCCAT GCAAGATTGT TTCTGTCCAT 
TTACAAGAAA CTACTCTGAA GGAGTTAAAA CAGGTGACAC CATCTATGAA AGAGAAGAGT 
GTAAGCATTA CACAGATGAA TGAAGTAACT CCTGATACTT TTGGGACTAC TGTTAAAACA 
CAATTAGGAG TATCTGAGTC TGCAGGACAG GGTGATTCAG AGGCAAAAGA AGAGAAATCT 
AAGCTAGTGA AAGAAAATGG AGATGAATCC AACAGCCTAT CTGACAAAGA GACAGGTTTT 
GGAGAACATC ATCACTTGCA TCTTTCCAGC TGTCGTGAAT GTCTGCAACT TGAAAACAGC 
ACTATTGAAT CAGTCAGATT TGCCTCTGCG GAAGACATTC CAGAACTTCC TGATGATTGC 
AATAGCAAAC TGGAAGAGAA TGGTGATTGT CTGACTACAG GAATAAAGAG AGTAAACCTT 
GCTGGAAAGC CTCCTAATAT ATTGATTTAT GTTGGCTCTG GAACTGCAAA AGTGGACTTT 
GAGCAAGTAA AATCAATCAT TCAGGAATGT GTTGATACAG ACAGCTATAC CATCTATCAG 
CTGCATGAGG AACAAGTTCT GAAAGCTCCG TGGATTGATA ATTCACTGCT GTTGATCATT 
GCCACAGAGG GGCCTATTTC TGAGAAGAAC CAAAAACAGT TTATGAAGTT TTTATCTAAA 
GGTGGAAAGA TTCTAGGGTT ATCTTCCTCT TTTACATTTG ATGGTATACA AATAAAGAGG 
AAAGACAAAC TGAAGAGAAC TGTTCATGAA TTAGTGGTCT CTAAAATGGA CAGTACTGAA 
ATGAAGTTAA ATCTTTTGAT CAGTGGCTGT ATTTTTGAGG TAGCGATGAA GGAAGGGAGC 
AGCAAAGTGA AGCCATTGAG TCGCTTAAAT AATGCTGATA AAGATATAGT TATTGTTTAT 
CTGCCTTATG GAGACAATGG AGGAGAAGCC ATTCTTTCTC AGGTGCATTT GGAACTGGAT 
ACCAATTCTG TGGATATCCA AACTGAAGAG GATTTTAATT TGCTTAAGAT GAGCAATCCC 
AAGAGATATG AAGTTCTCAA GGAGATTCTG ATATCACTTG GGCTGAGTTG TGAATTAAGT 
GAGATTCCTA TGTTGACGCC TATTTACCTT CTCTCTTCTG ATGAGGAAAT CCATCTTACG 
TTTTTGAAAT GGCTTGAGGA GAATGTAAAT GTTGAAGGAT TAATAGAATC CAGCAAAGTG 
TCTCTTAAAT TTGTTTCATC TTGTGAATCG AAAATGGAGA TAACTCCATC TCTCATGTCA 
GTCATCACCA AGATGGGAAA CTTCTCATCA GAACATTTCA GCCTGGAGAC ATATCAGCAG 
AATCTACAAA CAAAGAAACT TGGAAAGATT CTTTTCTTTA CTGAAGTTAC CACCACAACA 
ATGAATCTTC TAGACGGGTT AATGTTCAAG TTGCCTGAGG AGATGGGTTT AATAGCAATT 
GCTGTTCGAC AAACACAAGG GAAAGGTCGT GGCGGAAATG TTTGGCTCAG TCCCATTGGT 
TGTGCACTAT CCACTTTACA TATCACAATC CCTTTACATT CCAACTTGGG CCAGAGAATT 
CCTTTTATTC AACACCTAGT GTCCTTGGCA GTGGTAGAGT CGGTTAGATC AATACCAGGA 
TATGAGGATA TTGACCTGCG AGTAAAATGG CCAAATGATA TTTATTACAG TGATCTTATG 
AAGCTTGGTG GAGTTCTGGT AACCTCTACA CTTATTGAGA CAACATTTCA TATACTTATT 
GGCTTTGGAT TTAATGTGAA TAACAGCAAC CCCACCATAT GCATCAATGA CCTCATCACA 
AAATTTAATA AGGAAGAGGG GACAAATCTG AAGGCTTTAA CTGCAGACTG CCTTATTGCG 
AGAACTGTAA CTGTGCTAGA GAGGCTTATA GATATTTTTC AGGAGAAAGG GCCCAATGGA 
GTTCTTCCTC GTTACTACAA GTACTGGGTA CACAGTGGTA AGCAGGTTCG TCTCCACAAT 
GAGGAGGGCC CGTTGGCATG GATAGTTGGG ATTGATGATT ATGGATTCCT TCAAGTTCAT 
GAAGAAGGCA AAGGTGTAGA GTCTGTGCAT CCAGATGGCA ACTCTTTTGA CATGCTGAAA 
AACCTCATAA TCCCAAAGCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_025145239.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001007.1 Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa30361
Clone ID Related Accession (Same CDS sequence)	XM_015301496.1 , XM_015301496.2 , XM_025145239.1
Accession Version	XM_025145239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	biotin--protein ligase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA GGCTTCAAAT GGATAACGGA ATGGTTCCAT GCAAGATTGT TTCTGTCCAT 
TTACAAGAAA CTACTCTGAA GGAGTTAAAA CAGGTGACAC CATCTATGAA AGAGAAGAGT 
GTAAGCATTA CACAGATGAA TGAAGTAACT CCTGATACTT TTGGGACTAC TGTTAAAACA 
CAATTAGGAG TATCTGAGTC TGCAGGACAG GGTGATTCAG AGGCAAAAGA AGAGAAATCT 
AAGCTAGTGA AAGAAAATGG AGATGAATCC AACAGCCTAT CTGACAAAGA GACAGGTTTT 
GGAGAACATC ATCACTTGCA TCTTTCCAGC TGTCGTGAAT GTCTGCAACT TGAAAACAGC 
ACTATTGAAT CAGTCAGATT TGCCTCTGCG GAAGACATTC CAGAACTTCC TGATGATTGC 
AATAGCAAAC TGGAAGAGAA TGGTGATTGT CTGACTACAG GAATAAAGAG AGTAAACCTT 
GCTGGAAAGC CTCCTAATAT ATTGATTTAT GTTGGCTCTG GAACTGCAAA AGTGGACTTT 
GAGCAAGTAA AATCAATCAT TCAGGAATGT GTTGATACAG ACAGCTATAC CATCTATCAG 
CTGCATGAGG AACAAGTTCT GAAAGCTCCG TGGATTGATA ATTCACTGCT GTTGATCATT 
GCCACAGAGG GGCCTATTTC TGAGAAGAAC CAAAAACAGT TTATGAAGTT TTTATCTAAA 
GGTGGAAAGA TTCTAGGGTT ATCTTCCTCT TTTACATTTG ATGGTATACA AATAAAGAGG 
AAAGACAAAC TGAAGAGAAC TGTTCATGAA TTAGTGGTCT CTAAAATGGA CAGTACTGAA 
ATGAAGTTAA ATCTTTTGAT CAGTGGCTGT ATTTTTGAGG TAGCGATGAA GGAAGGGAGC 
AGCAAAGTGA AGCCATTGAG TCGCTTAAAT AATGCTGATA AAGATATAGT TATTGTTTAT 
CTGCCTTATG GAGACAATGG AGGAGAAGCC ATTCTTTCTC AGGTGCATTT GGAACTGGAT 
ACCAATTCTG TGGATATCCA AACTGAAGAG GATTTTAATT TGCTTAAGAT GAGCAATCCC 
AAGAGATATG AAGTTCTCAA GGAGATTCTG ATATCACTTG GGCTGAGTTG TGAATTAAGT 
GAGATTCCTA TGTTGACGCC TATTTACCTT CTCTCTTCTG ATGAGGAAAT CCATCTTACG 
TTTTTGAAAT GGCTTGAGGA GAATGTAAAT GTTGAAGGAT TAATAGAATC CAGCAAAGTG 
TCTCTTAAAT TTGTTTCATC TTGTGAATCG AAAATGGAGA TAACTCCATC TCTCATGTCA 
GTCATCACCA AGATGGGAAA CTTCTCATCA GAACATTTCA GCCTGGAGAC ATATCAGCAG 
AATCTACAAA CAAAGAAACT TGGAAAGATT CTTTTCTTTA CTGAAGTTAC CACCACAACA 
ATGAATCTTC TAGACGGGTT AATGTTCAAG TTGCCTGAGG AGATGGGTTT AATAGCAATT 
GCTGTTCGAC AAACACAAGG GAAAGGTCGT GGCGGAAATG TTTGGCTCAG TCCCATTGGT 
TGTGCACTAT CCACTTTACA TATCACAATC CCTTTACATT CCAACTTGGG CCAGAGAATT 
CCTTTTATTC AACACCTAGT GTCCTTGGCA GTGGTAGAGT CGGTTAGATC AATACCAGGA 
TATGAGGATA TTGACCTGCG AGTAAAATGG CCAAATGATA TTTATTACAG TGATCTTATG 
AAGCTTGGTG GAGTTCTGGT AACCTCTACA CTTATTGAGA CAACATTTCA TATACTTATT 
GGCTTTGGAT TTAATGTGAA TAACAGCAAC CCCACCATAT GCATCAATGA CCTCATCACA 
AAATTTAATA AGGAAGAGGG GACAAATCTG AAGGCTTTAA CTGCAGACTG CCTTATTGCG 
AGAACTGTAA CTGTGCTAGA GAGGCTTATA GATATTTTTC AGGAGAAAGG GCCCAATGGA 
GTTCTTCCTC GTTACTACAA GTACTGGGTA CACAGTGGTA AGCAGGTTCG TCTCCACAAT 
GAGGAGGGCC CGTTGGCATG GATAGTTGGG ATTGATGATT ATGGATTCCT TCAAGTTCAT 
GAAGAAGGCA AAGGTGTAGA GTCTGTGCAT CCAGATGGCA ACTCTTTTGA CATGCTGAAA 
AACCTCATAA TCCCAAAGCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001007.1
CDS	368..2551
Translation	MEDRLQMDNGMVPCKIVSVHLQETTLKELKQVTPSMKEKSVSITQMNEVTPDTFGTTVKTQLGVSESAGQGDSEAKEEKSKLVKENGDESNSLSDKETGFGEHHHLHLSSCRECLQLENSTIESVRFASAEDIPELPDDCNSKLEENGDCLTTGIKRVNLAGKPPNILIYVGSGTAKVDFEQVKSIIQECVDTDSYTIYQLHEEQVLKAPWIDNSLLLIIATEGPISEKNQKQFMKFLSKGGKILGLSSSFTFDGIQIKRKDKLKRTVHELVVSKMDSTEMKLNLLISGCIFEVAMKEGSSKVKPLSRLNNADKDIVIVYLPYGDNGGEAILSQVHLELDTNSVDIQTEEDFNLLKMSNPKRYEVLKEILISLGLSCELSEIPMLTPIYLLSSDEEIHLTFLKWLEENVNVEGLIESSKVSLKFVSSCESKMEITPSLMSVITKMGNFSSEHFSLETYQQNLQTKKLGKILFFTEVTTTTMNLLDGLMFKLPEEMGLIAIAVRQTQGKGRGGNVWLSPIGCALSTLHITIPLHSNLGQRIPFIQHLVSLAVVESVRSIPGYEDIDLRVKWPNDIYYSDLMKLGGVLVTSTLIETTFHILIGFGFNVNNSNPTICINDLITKFNKEEGTNLKALTADCLIARTVTVLERLIDIFQEKGPNGVLPRYYKYWVHSGKQVRLHNEEGPLAWIVGIDDYGFLQVHEEGKGVESVHPDGNSFDMLKNLIIPKH

Target ORF information:

RefSeq Version	XM_025145239.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145239.1

ORF Insert Sequence:

ATGGAAGACA GGCTTCAAAT GGATAACGGA ATGGTTCCAT GCAAGATTGT TTCTGTCCAT 
TTACAAGAAA CTACTCTGAA GGAGTTAAAA CAGGTGACAC CATCTATGAA AGAGAAGAGT 
GTAAGCATTA CACAGATGAA TGAAGTAACT CCTGATACTT TTGGGACTAC TGTTAAAACA 
CAATTAGGAG TATCTGAGTC TGCAGGACAG GGTGATTCAG AGGCAAAAGA AGAGAAATCT 
AAGCTAGTGA AAGAAAATGG AGATGAATCC AACAGCCTAT CTGACAAAGA GACAGGTTTT 
GGAGAACATC ATCACTTGCA TCTTTCCAGC TGTCGTGAAT GTCTGCAACT TGAAAACAGC 
ACTATTGAAT CAGTCAGATT TGCCTCTGCG GAAGACATTC CAGAACTTCC TGATGATTGC 
AATAGCAAAC TGGAAGAGAA TGGTGATTGT CTGACTACAG GAATAAAGAG AGTAAACCTT 
GCTGGAAAGC CTCCTAATAT ATTGATTTAT GTTGGCTCTG GAACTGCAAA AGTGGACTTT 
GAGCAAGTAA AATCAATCAT TCAGGAATGT GTTGATACAG ACAGCTATAC CATCTATCAG 
CTGCATGAGG AACAAGTTCT GAAAGCTCCG TGGATTGATA ATTCACTGCT GTTGATCATT 
GCCACAGAGG GGCCTATTTC TGAGAAGAAC CAAAAACAGT TTATGAAGTT TTTATCTAAA 
GGTGGAAAGA TTCTAGGGTT ATCTTCCTCT TTTACATTTG ATGGTATACA AATAAAGAGG 
AAAGACAAAC TGAAGAGAAC TGTTCATGAA TTAGTGGTCT CTAAAATGGA CAGTACTGAA 
ATGAAGTTAA ATCTTTTGAT CAGTGGCTGT ATTTTTGAGG TAGCGATGAA GGAAGGGAGC 
AGCAAAGTGA AGCCATTGAG TCGCTTAAAT AATGCTGATA AAGATATAGT TATTGTTTAT 
CTGCCTTATG GAGACAATGG AGGAGAAGCC ATTCTTTCTC AGGTGCATTT GGAACTGGAT 
ACCAATTCTG TGGATATCCA AACTGAAGAG GATTTTAATT TGCTTAAGAT GAGCAATCCC 
AAGAGATATG AAGTTCTCAA GGAGATTCTG ATATCACTTG GGCTGAGTTG TGAATTAAGT 
GAGATTCCTA TGTTGACGCC TATTTACCTT CTCTCTTCTG ATGAGGAAAT CCATCTTACG 
TTTTTGAAAT GGCTTGAGGA GAATGTAAAT GTTGAAGGAT TAATAGAATC CAGCAAAGTG 
TCTCTTAAAT TTGTTTCATC TTGTGAATCG AAAATGGAGA TAACTCCATC TCTCATGTCA 
GTCATCACCA AGATGGGAAA CTTCTCATCA GAACATTTCA GCCTGGAGAC ATATCAGCAG 
AATCTACAAA CAAAGAAACT TGGAAAGATT CTTTTCTTTA CTGAAGTTAC CACCACAACA 
ATGAATCTTC TAGACGGGTT AATGTTCAAG TTGCCTGAGG AGATGGGTTT AATAGCAATT 
GCTGTTCGAC AAACACAAGG GAAAGGTCGT GGCGGAAATG TTTGGCTCAG TCCCATTGGT 
TGTGCACTAT CCACTTTACA TATCACAATC CCTTTACATT CCAACTTGGG CCAGAGAATT 
CCTTTTATTC AACACCTAGT GTCCTTGGCA GTGGTAGAGT CGGTTAGATC AATACCAGGA 
TATGAGGATA TTGACCTGCG AGTAAAATGG CCAAATGATA TTTATTACAG TGATCTTATG 
AAGCTTGGTG GAGTTCTGGT AACCTCTACA CTTATTGAGA CAACATTTCA TATACTTATT 
GGCTTTGGAT TTAATGTGAA TAACAGCAAC CCCACCATAT GCATCAATGA CCTCATCACA 
AAATTTAATA AGGAAGAGGG GACAAATCTG AAGGCTTTAA CTGCAGACTG CCTTATTGCG 
AGAACTGTAA CTGTGCTAGA GAGGCTTATA GATATTTTTC AGGAGAAAGG GCCCAATGGA 
GTTCTTCCTC GTTACTACAA GTACTGGGTA CACAGTGGTA AGCAGGTTCG TCTCCACAAT 
GAGGAGGGCC CGTTGGCATG GATAGTTGGG ATTGATGATT ATGGATTCCT TCAAGTTCAT 
GAAGAAGGCA AAGGTGTAGA GTCTGTGCAT CCAGATGGCA ACTCTTTTGA CATGCTGAAA 
AACCTCATAA TCCCAAAGCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HLCS cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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