HLCS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HLCS
Entrez Gene ID	3141
Full Name	holocarboxylase synthetase
Synonyms	HCS
General protein information	Preferred Names holocarboxylase synthetase Names biotin--protein ligase biotin apo-protein ligase biotin--[acetyl-CoA-carboxylase] ligase biotin--[methylcrotonoyl-CoA-carboxylase] ligase biotin--[methylmalonyl-CoA-carboxytransferase] ligase holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q22.13
Summary	This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jun 2011].
Disorder MIM:	609018
Disorder Html:	Holocarboxylase synthetase deficiency, 253270 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006723994.2	XP_006724057.1	biotin--protein ligase isoform X2
NM_001352515.1	NP_001339444.1	biotin--protein ligase isoform 1
NM_000411.8	NP_000402.3	biotin--protein ligase isoform 1
NM_001352516.2	NP_001339445.1	biotin--protein ligase isoform 1
NM_001352518.1	NP_001339447.1	biotin--protein ligase isoform 1
XM_011529538.1	XP_011527840.1	biotin--protein ligase isoform X1
NM_001352517.1	NP_001339446.1	biotin--protein ligase isoform 1
NM_001352515.2	NP_001339444.1	biotin--protein ligase isoform 1
XM_005260953.3	XP_005261010.1	biotin--protein ligase isoform X1
NM_000411.6	NP_000402.3	biotin--protein ligase
NM_001242784.2	NP_001229713.1	biotin--protein ligase isoform 1
NM_001242785.1	NP_001229714.1	biotin--protein ligase
NM_000411.7	NP_000402.3	biotin--protein ligase isoform 1
NM_001242784.3	NP_001229713.1	biotin--protein ligase isoform 1
XM_006723995.1	XP_006724058.1	biotin--protein ligase isoform X1
NM_001352514.2	NP_001339443.1	biotin--protein ligase isoform 2
XM_005260955.3	XP_005261012.1	biotin--protein ligase isoform X2
NM_001352514.1	NP_001339443.1	biotin--protein ligase isoform 2
XM_024452065.1	XP_024307833.1	biotin--protein ligase isoform X2
XM_017028330.1	XP_016883819.1	biotin--protein ligase isoform X1
NM_001352516.1	NP_001339445.1	biotin--protein ligase isoform 1
NM_001242784.1	NP_001229713.1	biotin--protein ligase
XM_011529539.2	XP_011527841.1	biotin--protein ligase isoform X2
XM_011529541.2	XP_011527843.1	biotin--protein ligase isoform X2
XM_005260956.3	XP_005261013.1	biotin--protein ligase isoform X2
XM_011529539.3	XP_011527841.1	biotin--protein ligase isoform X1
XM_024452066.1	XP_024307834.1	biotin--protein ligase isoform X2
XM_011529540.2	XP_011527842.1	biotin--protein ligase isoform X3
NM_001242785.2	NP_001229714.1	biotin--protein ligase isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HLCS	NP_000402.3
Arabidopsis thaliana (thale cress)	HCS1	NP_565605.1
Xenopus tropicalis (tropical clawed frog)	hlcs	XP_004912175.1
Pan troglodytes (chimpanzee)	HLCS	XP_003319106.2
	HLCS	XP_001084173.2
Bos taurus (cattle)	HLCS	XP_005202184.1
Arabidopsis thaliana (thale cress)	HCS2	NP_850957.1
Mus musculus (house mouse)	Hlcs	NP_631884.1
Gallus gallus (chicken)	HLCS	XP_004934596.1
Canis lupus familiaris (dog)	HLCS	XP_005638959.1
Rattus norvegicus (Norway rat)	Hlcs	XP_006248175.1
Caenorhabditis elegans (roundworm)	bpl-1	NP_871920.1
Danio rerio (zebrafish)	hlcs	XP_002663496.3

Gene Ontology
Bibliography

Related articles in PubMed

Holocarboxylase Synthetase: A Moonlighting Transcriptional Coregulator of Gene Expression and a Cytosolic Regulator of Biotin Utilization.
Le?n-Del-R?o A, Valadez-Graham V, Gravel RA
Annual review of nutrition37207-223(2017 Aug)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Holocarboxylase synthetase interacts physically with nuclear receptor co-repressor, histone deacetylase 1 and a novel splicing variant of histone deacetylase 1 to repress repeats.
Liu D, Zempleni J
The Biochemical journal461(3)477-86(2014 Aug)

Holocarboxylase synthetase acts as a biotin-independent transcriptional repressor interacting with HDAC1, HDAC2 and HDAC7.
Trujillo-Gonzalez I, Cervantes-Roldan R, Gonzalez-Noriega A, Michalak C, Reyes-Carmona S, Barrios-Garcia T, Meneses-Morales I, Leon-Del-Rio A
Molecular genetics and metabolism111(3)321-330(2014 Mar)

Holocarboxylase synthetase catalyzes biotinylation of heat shock protein 72, thereby inducing RANTES expression in HEK-293 cells.
Xue J, Zhou J, Zempleni J
American journal of physiology. Cell physiology305(12)C1240-5(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The function of HCS and biotin in metabolism and human disease, a putative role for the enzyme in histone biotinylation, and its participation as a nuclear factor in chromatin dynamics is discussed.
Title: Holocarboxylase Synthetase: A Moonlighting Transcriptional Coregulator of Gene Expression and a Cytosolic Regulator of Biotin Utilization.

Data suggest that direct interactions of HLCS (holocarboxylase synthetase) with NCOR1 (nuclear receptor corepressor 1) and HDAC1 (histone deacetylase 1) contribute toward transcriptional repression of repeats, presumably increasing genome stability.
Title: Holocarboxylase synthetase interacts physically with nuclear receptor co-repressor, histone deacetylase 1 and a novel splicing variant of histone deacetylase 1 to repress repeats.

The transcriptional function of HCS was shown by in vitro pull down and in vivo co-immunoprecipitation assays to depend on its interaction with the histone deacetylases HDAC1, HDAC2 and HDAC7
Title: Holocarboxylase synthetase acts as a biotin-independent transcriptional repressor interacting with HDAC1, HDAC2 and HDAC7.

This study showed that modification of HSP72 and demonstrated that binding of biotin to extracellular HSP72 prepares cells for a strong immune response.
Title: Holocarboxylase synthetase catalyzes biotinylation of heat shock protein 72, thereby inducing RANTES expression in HEK-293 cells.

study characterizes three functional promoters in the human HLCS gene
Title: Three promoters regulate the transcriptional activity of the human holocarboxylase synthetase gene.

The following HLCS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLCS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22818	NM_001242785.2 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu22818	NM_001352517.1 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	NM_001352518.1 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	NM_001352516.2 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	NM_001352515.2 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	NM_001242784.3 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	NM_000411.8 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	XM_011529538.1 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	XM_006723994.2 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	XM_006723995.1 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	XM_011529539.3 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	XM_005260956.3 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	XM_011529541.2 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	XM_017028330.1 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	XM_005260955.3 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu105174	XM_024452066.1 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu105174	XM_024452065.1 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu53235	NM_001352514.2 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu53235	XM_005260953.3 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu58262	XM_011529540.2 Latest version!	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu22818	NM_001352515.1	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	NM_000411.6	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	NM_000411.7	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	NM_001242784.2	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu53235	NM_001352514.1	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu22818	NM_001352516.1	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	NM_001242784.1	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	XM_011529539.2	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu22818	NM_001242785.1	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HLCS ( NM_001242785.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229714.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_001242785.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, AP000703.1, AP000702.1, AP000698.1 and AP000697.1. On Jun 16, 2017 this sequence version replaced NM_001242785.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229714.1
CDS	604..2784
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001242785.2
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242785.2

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_001352515.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339444.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_001352515.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, AP000703.1, AP000702.1, AP000698.1 and AP000697.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.20706.1, SRR1803611.151304.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339444.1
CDS	484..2664
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001352515.1
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352515.1

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_000411.8 ) cDNA ORF clone, Homo sapiens(human) -> NP_000402.3 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_000411.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D23672.2, D87328.1 and AP000697.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_000411.7. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.223049.1, D23672.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000402.3
CDS	447..2627
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_000411.8
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000411.8

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_001352516.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339445.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_001352516.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, AP000703.1, AP000702.1, AP000698.1 and AP000697.1. On Jun 2, 2019 this sequence version replaced NM_001352516.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.176063.1, SRR1803616.79621.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339445.1
CDS	504..2684
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001352516.2
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352516.2

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_001352518.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339447.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_001352518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, AP000703.1, AP000702.1, AP000698.1 and AP000697.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339447.1
CDS	317..2497
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001352518.1
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352518.1

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_001352517.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339446.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_001352517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, AP000703.1, AP000702.1, AP000698.1 and AP000697.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.173657.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339446.1
CDS	458..2638
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001352517.1
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352517.1

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_001352515.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339444.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_001352515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, AP000703.1, AP000702.1, AP000698.1 and AP000697.1. On Jun 2, 2019 this sequence version replaced NM_001352515.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.20706.1, SRR1803611.151304.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339444.1
CDS	301..2481
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001352515.2
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352515.2

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_000411.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_000402.3 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_000411.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-15
Organism	Homo sapiens(Human)
Product	biotin--protein ligase
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA334377.1, D23672.2, D87328.1 and AP000697.1. This sequence is a reference standard in the RefSeqGene project. On Jun 28, 2011 this sequence version replaced NM_000411.5. Transcript Variant: This variant (1) is the shortest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D23672.2, BC060787.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000402.3
CDS	481..2661
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_000411.6
Organism	Homo sapiens(Human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000411.6

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_001242784.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229713.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_001242784.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, DB290499.1, D87328.1, AP000698.1 and AP000697.1. On Jun 16, 2017 this sequence version replaced NM_001242784.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D87328.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229713.1
CDS	1449..3629
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001242784.2
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242784.2

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_001242785.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229714.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_001242785.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-15
Organism	Homo sapiens(Human)
Product	biotin--protein ligase
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, AP000703.1, AP000702.1, AP000698.1 and AP000697.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: D87328.1, D23672.2 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229714.1
CDS	574..2754
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001242785.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242785.1

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_000411.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_000402.3 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_000411.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, DA334377.1, D23672.2, D87328.1 and AP000697.1. This sequence is a reference standard in the RefSeqGene project. On Jun 16, 2017 this sequence version replaced NM_000411.6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.223049.1, D23672.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000402.3
CDS	630..2810
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_000411.7
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000411.7

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_001242784.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229713.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_001242784.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, DB290499.1, D87328.1, AP000698.1 and AP000697.1. On Jun 2, 2019 this sequence version replaced NM_001242784.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D87328.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229713.1
CDS	1428..3608
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001242784.3
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242784.3

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_001352516.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339445.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_001352516.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, AP000703.1, AP000702.1, AP000698.1 and AP000697.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.176063.1, SRR1660809.121356.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339445.1
CDS	504..2684
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001352516.1
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352516.1

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_001242784.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229713.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	NM_001242784.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-15
Organism	Homo sapiens(Human)
Product	biotin--protein ligase
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB290499.1, D87328.1, AP000698.1 and AP000697.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D87328.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229713.1
CDS	1415..3595
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001242784.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242784.1

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_006723994.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724057.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	XM_006723994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724057.1
CDS	428..2608
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	XM_006723994.2
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723994.2

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_011529538.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527840.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	XM_011529538.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527840.1
CDS	461..2641
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	XM_011529538.1
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529538.1

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_006723995.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724058.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	XM_006723995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724058.1
CDS	606..2786
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	XM_006723995.1
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723995.1

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_005260955.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005261012.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	XM_005260955.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260955.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005261012.1
CDS	314..2494
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	XM_005260955.3
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260955.3

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_017028330.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883819.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	XM_017028330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883819.1
CDS	264..2444
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	XM_017028330.1
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028330.1

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_011529539.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527841.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	XM_011529539.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527841.1
CDS	466..2646
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	XM_011529539.2
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529539.2

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_011529541.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527843.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	XM_011529541.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529541.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527843.1
CDS	317..2497
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	XM_011529541.2
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529541.2

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_005260956.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005261013.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	XM_005260956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005261013.1
CDS	490..2670
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	XM_005260956.3
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260956.3

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_011529539.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527841.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu22818
Clone ID Related Accession (Same CDS sequence)	XM_006723994.2 , NM_001352515.1 , NM_000411.8 , NM_001352516.2 , NM_001352518.1 , XM_011529538.1 , NM_001352517.1 , NM_001352515.2 , NM_000411.6 , NM_001242784.2 , NM_001242785.1 , NM_000411.7 , NM_001242784.3 , XM_006723995.1 , XM_005260955.3 , XM_017028330.1 , NM_001352516.1 , NM_001242784.1 , XM_011529539.2 , XM_011529541.2 , XM_005260956.3 , XM_011529539.3 , NM_001242785.2
Accession Version	XM_011529539.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011529539.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527841.1
CDS	467..2647
Translation	MEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	XM_011529539.3
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529539.3

ORF Insert Sequence:

ATGGAAGATA GACTCCACAT GGATAATGGA CTGGTACCCC AAAAGATTGT GTCGGTGCAC 
TTGCAGGACT CCACTCTGAA GGAAGTTAAG GATCAGGTCT CAAACAAGCA AGCCCAGATC 
CTAGAGCCGA AGCCTGAACC TTCTCTTGAG ATTAAGCCTG AGCAGGACGG TATGGAGCAT 
GTTGGCAGAG ATGACCCAAA GGCTCTTGGT GAAGAACCCA AACAAAGGAG AGGCAGTGCC 
TCTGGGAGTG AGCCTGCTGG GGACAGTGAC AGGGGAGGGG GCCCCGTTGA GCATTATCAC 
CTCCATCTGT CTAGTTGCCA CGAGTGTCTG GAACTTGAGA ACAGCACCAT TGAGTCAGTC 
AAGTTTGCGT CTGCCGAGAA CATTCCAGAC CTTCCCTACG ATTATAGCAG CAGTTTGGAG 
AGTGTTGCTG ATGAGACCTC CCCCGAAAGA GAAGGGAGGA GAGTCAACCT CACGGGAAAG 
GCACCCAACA TCCTCCTCTA TGTGGGCTCC GACTCCCAGG AAGCCCTCGG CCGGTTCCAC 
GAGGTCCGGT CTGTGCTGGC CGACTGTGTG GACATTGACA GTTATATTCT CTACCACCTG 
CTGGAGGACA GTGCTCTCAG AGACCCGTGG ACGGACAACT GTCTGCTGTT GGTCATTGCT 
ACCAGGGAGT CCATTCCCGA AGACCTGTAC CAGAAGTTCA TGGCCTATCT TTCTCAGGGA 
GGGAAGGTGT TGGGCCTGTC TTCATCCTTC ACCTTTGGTG GCTTTCAGGT GACAAGCAAG 
GGTGCACTGC ACAAGACAGT CCAGAACTTG GTTTTCTCCA AGGCTGACCA GAGCGAGGTG 
AAGCTCAGCG TCTTGAGCAG TGGCTGCAGG TACCAGGAAG GCCCCGTCCG GCTCAGCCCC 
GGCAGGCTCC AGGGCCACCT GGAGAATGAG GACAAGGACA GGATGATTGT GCATGTGCCT 
TTTGGAACTC GCGGGGGAGA AGCTGTTCTT TGCCAGGTGC ACTTAGAACT ACCTCCCAGC 
TCCAACATAG TGCAAACTCC AGAAGATTTT AACTTGCTCA AGTCAAGCAA TTTTAGAAGA 
TACGAAGTCC TTAGAGAGAT TCTGACAACC CTTGGCCTCA GCTGTGACAT GAAACAAGTT 
CCTGCCTTAA CTCCTCTTTA CTTGCTGTCA GCTGCGGAGG AAATCAGGGA TCCTCTTATG 
CAGTGGCTTG GGAAACATGT GGACTCCGAG GGAGAAATAA AATCCGGCCA GCTCTCTCTT 
AGATTTGTTT CATCCTACGT GTCTGAAGTA GAAATAACCC CATCTTGTAT ACCTGTGGTG 
ACCAACATGG AGGCCTTCTC ATCAGAACAT TTCAACTTAG AGATCTATCG CCAAAATCTG 
CAGACCAAGC AGTTGGGGAA AGTAATTTTG TTTGCCGAAG TGACCCCCAC AACGATGCGT 
CTCCTGGATG GGCTGATGTT TCAGACACCG CAGGAAATGG GCTTAATAGT GATCGCGGCC 
CGGCAGACCG AGGGCAAAGG ACGGGGAGGG AATGTGTGGC TGAGCCCTGT GGGATGTGCT 
CTTTCTACTC TGCTCATCTC CATTCCACTG AGATCCCAGC TGGGACAGAG GATCCCGTTT 
GTCCAGCATC TGATGTCCGT GGCTGTCGTG GAAGCAGTGA GGTCCATTCC CGAGTATCAG 
GATATCAACT TACGAGTGAA GTGGCCCAAC GATATTTATT ACAGTGACCT CATGAAGATC 
GGCGGAGTTC TGGTTAACTC AACACTCATG GGAGAAACAT TTTATATACT TATTGGCTGT 
GGATTTAATG TGACTAACAG TAACCCTACC ATCTGCATCA ACGACCTCAT CACAGAATAC 
AATAAACAAC ACAAGGCAGA ACTGAAGCCC TTAAGAGCCG ATTATCTCAT CGCCAGAGTC 
GTGACTGTGC TGGAGAAACT GATCAAAGAG TTTCAGGACA AAGGGCCCAA CAGCGTCCTT 
CCCCTTTATT ACCGATACTG GGTCCACAGT GGTCAGCAAG TCCATCTGGG CAGCGCAGAG 
GGACCAAAGG TGTCCATCGT TGGCCTGGAC GATTCTGGCT TCCTCCAGGT TCACCAGGAG 
GGCGGCGAGG TTGTGACTGT GCACCCGGAC GGCAACTCCT TCGACATGCT GAGAAACCTC 
ATCCTCCCCA AACGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_024452065.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307833.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu105174
Clone ID Related Accession (Same CDS sequence)	XM_024452065.1 , XM_024452066.1
Accession Version	XM_024452065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATG TTGGCAGAGA TGACCCAAAG GCTCTTGGTG AAGAACCCAA ACAAAGGAGA 
GGCAGTGCCT CTGGGAGTGA GCCTGCTGGG GACAGTGACA GGGGAGGGGG CCCCGTTGAG 
CATTATCACC TCCATCTGTC TAGTTGCCAC GAGTGTCTGG AACTTGAGAA CAGCACCATT 
GAGTCAGTCA AGTTTGCGTC TGCCGAGAAC ATTCCAGACC TTCCCTACGA TTATAGCAGC 
AGTTTGGAGA GTGTTGCTGA TGAGACCTCC CCCGAAAGAG AAGGGAGGAG AGTCAACCTC 
ACGGGAAAGG CACCCAACAT CCTCCTCTAT GTGGGCTCCG ACTCCCAGGA AGCCCTCGGC 
CGGTTCCACG AGGTCCGGTC TGTGCTGGCC GACTGTGTGG ACATTGACAG TTATATTCTC 
TACCACCTGC TGGAGGACAG TGCTCTCAGA GACCCGTGGA CGGACAACTG TCTGCTGTTG 
GTCATTGCTA CCAGGGAGTC CATTCCCGAA GACCTGTACC AGAAGTTCAT GGCCTATCTT 
TCTCAGGGAG GGAAGGTGTT GGGCCTGTCT TCATCCTTCA CCTTTGGTGG CTTTCAGGTG 
ACAAGCAAGG GTGCACTGCA CAAGACAGTC CAGAACTTGG TTTTCTCCAA GGCTGACCAG 
AGCGAGGTGA AGCTCAGCGT CTTGAGCAGT GGCTGCAGGT ACCAGGAAGG CCCCGTCCGG 
CTCAGCCCCG GCAGGCTCCA GGGCCACCTG GAGAATGAGG ACAAGGACAG GATGATTGTG 
CATGTGCCTT TTGGAACTCG CGGGGGAGAA GCTGTTCTTT GCCAGGTGCA CTTAGAACTA 
CCTCCCAGCT CCAACATAGT GCAAACTCCA GAAGATTTTA ACTTGCTCAA GTCAAGCAAT 
TTTAGAAGAT ACGAAGTCCT TAGAGAGATT CTGACAACCC TTGGCCTCAG CTGTGACATG 
AAACAAGTTC CTGCCTTAAC TCCTCTTTAC TTGCTGTCAG CTGCGGAGGA AATCAGGGAT 
CCTCTTATGC AGTGGCTTGG GAAACATGTG GACTCCGAGG GAGAAATAAA ATCCGGCCAG 
CTCTCTCTTA GATTTGTTTC ATCCTACGTG TCTGAAGTAG AAATAACCCC ATCTTGTATA 
CCTGTGGTGA CCAACATGGA GGCCTTCTCA TCAGAACATT TCAACTTAGA GATCTATCGC 
CAAAATCTGC AGACCAAGCA GTTGGGGAAA GTAATTTTGT TTGCCGAAGT GACCCCCACA 
ACGATGCGTC TCCTGGATGG GCTGATGTTT CAGACACCGC AGGAAATGGG CTTAATAGTG 
ATCGCGGCCC GGCAGACCGA GGGCAAAGGA CGGGGAGGGA ATGTGTGGCT GAGCCCTGTG 
GGATGTGCTC TTTCTACTCT GCTCATCTCC ATTCCACTGA GATCCCAGCT GGGACAGAGG 
ATCCCGTTTG TCCAGCATCT GATGTCCGTG GCTGTCGTGG AAGCAGTGAG GTCCATTCCC 
GAGTATCAGG ATATCAACTT ACGAGTGAAG TGGCCCAACG ATATTTATTA CAGTGACCTC 
ATGAAGATCG GCGGAGTTCT GGTTAACTCA ACACTCATGG GAGAAACATT TTATATACTT 
ATTGGCTGTG GATTTAATGT GACTAACAGT AACCCTACCA TCTGCATCAA CGACCTCATC 
ACAGAATACA ATAAACAACA CAAGGCAGAA CTGAAGCCCT TAAGAGCCGA TTATCTCATC 
GCCAGAGTCG TGACTGTGCT GGAGAAACTG ATCAAAGAGT TTCAGGACAA AGGGCCCAAC 
AGCGTCCTTC CCCTTTATTA CCGATACTGG GTCCACAGTG GTCAGCAAGT CCATCTGGGC 
AGCGCAGAGG GACCAAAGGT GTCCATCGTT GGCCTGGACG ATTCTGGCTT CCTCCAGGTT 
CACCAGGAGG GCGGCGAGGT TGTGACTGTG CACCCGGACG GCAACTCCTT CGACATGCTG 
AGAAACCTCA TCCTCCCCAA ACGGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307833.1
CDS	353..2362
Translation	MEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	XM_024452065.1
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452065.1

ORF Insert Sequence:

ATGGAGCATG TTGGCAGAGA TGACCCAAAG GCTCTTGGTG AAGAACCCAA ACAAAGGAGA 
GGCAGTGCCT CTGGGAGTGA GCCTGCTGGG GACAGTGACA GGGGAGGGGG CCCCGTTGAG 
CATTATCACC TCCATCTGTC TAGTTGCCAC GAGTGTCTGG AACTTGAGAA CAGCACCATT 
GAGTCAGTCA AGTTTGCGTC TGCCGAGAAC ATTCCAGACC TTCCCTACGA TTATAGCAGC 
AGTTTGGAGA GTGTTGCTGA TGAGACCTCC CCCGAAAGAG AAGGGAGGAG AGTCAACCTC 
ACGGGAAAGG CACCCAACAT CCTCCTCTAT GTGGGCTCCG ACTCCCAGGA AGCCCTCGGC 
CGGTTCCACG AGGTCCGGTC TGTGCTGGCC GACTGTGTGG ACATTGACAG TTATATTCTC 
TACCACCTGC TGGAGGACAG TGCTCTCAGA GACCCGTGGA CGGACAACTG TCTGCTGTTG 
GTCATTGCTA CCAGGGAGTC CATTCCCGAA GACCTGTACC AGAAGTTCAT GGCCTATCTT 
TCTCAGGGAG GGAAGGTGTT GGGCCTGTCT TCATCCTTCA CCTTTGGTGG CTTTCAGGTG 
ACAAGCAAGG GTGCACTGCA CAAGACAGTC CAGAACTTGG TTTTCTCCAA GGCTGACCAG 
AGCGAGGTGA AGCTCAGCGT CTTGAGCAGT GGCTGCAGGT ACCAGGAAGG CCCCGTCCGG 
CTCAGCCCCG GCAGGCTCCA GGGCCACCTG GAGAATGAGG ACAAGGACAG GATGATTGTG 
CATGTGCCTT TTGGAACTCG CGGGGGAGAA GCTGTTCTTT GCCAGGTGCA CTTAGAACTA 
CCTCCCAGCT CCAACATAGT GCAAACTCCA GAAGATTTTA ACTTGCTCAA GTCAAGCAAT 
TTTAGAAGAT ACGAAGTCCT TAGAGAGATT CTGACAACCC TTGGCCTCAG CTGTGACATG 
AAACAAGTTC CTGCCTTAAC TCCTCTTTAC TTGCTGTCAG CTGCGGAGGA AATCAGGGAT 
CCTCTTATGC AGTGGCTTGG GAAACATGTG GACTCCGAGG GAGAAATAAA ATCCGGCCAG 
CTCTCTCTTA GATTTGTTTC ATCCTACGTG TCTGAAGTAG AAATAACCCC ATCTTGTATA 
CCTGTGGTGA CCAACATGGA GGCCTTCTCA TCAGAACATT TCAACTTAGA GATCTATCGC 
CAAAATCTGC AGACCAAGCA GTTGGGGAAA GTAATTTTGT TTGCCGAAGT GACCCCCACA 
ACGATGCGTC TCCTGGATGG GCTGATGTTT CAGACACCGC AGGAAATGGG CTTAATAGTG 
ATCGCGGCCC GGCAGACCGA GGGCAAAGGA CGGGGAGGGA ATGTGTGGCT GAGCCCTGTG 
GGATGTGCTC TTTCTACTCT GCTCATCTCC ATTCCACTGA GATCCCAGCT GGGACAGAGG 
ATCCCGTTTG TCCAGCATCT GATGTCCGTG GCTGTCGTGG AAGCAGTGAG GTCCATTCCC 
GAGTATCAGG ATATCAACTT ACGAGTGAAG TGGCCCAACG ATATTTATTA CAGTGACCTC 
ATGAAGATCG GCGGAGTTCT GGTTAACTCA ACACTCATGG GAGAAACATT TTATATACTT 
ATTGGCTGTG GATTTAATGT GACTAACAGT AACCCTACCA TCTGCATCAA CGACCTCATC 
ACAGAATACA ATAAACAACA CAAGGCAGAA CTGAAGCCCT TAAGAGCCGA TTATCTCATC 
GCCAGAGTCG TGACTGTGCT GGAGAAACTG ATCAAAGAGT TTCAGGACAA AGGGCCCAAC 
AGCGTCCTTC CCCTTTATTA CCGATACTGG GTCCACAGTG GTCAGCAAGT CCATCTGGGC 
AGCGCAGAGG GACCAAAGGT GTCCATCGTT GGCCTGGACG ATTCTGGCTT CCTCCAGGTT 
CACCAGGAGG GCGGCGAGGT TGTGACTGTG CACCCGGACG GCAACTCCTT CGACATGCTG 
AGAAACCTCA TCCTCCCCAA ACGGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_024452066.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307834.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X7, mRNA.

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CloneID	OHu105174
Clone ID Related Accession (Same CDS sequence)	XM_024452065.1 , XM_024452066.1
Accession Version	XM_024452066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATG TTGGCAGAGA TGACCCAAAG GCTCTTGGTG AAGAACCCAA ACAAAGGAGA 
GGCAGTGCCT CTGGGAGTGA GCCTGCTGGG GACAGTGACA GGGGAGGGGG CCCCGTTGAG 
CATTATCACC TCCATCTGTC TAGTTGCCAC GAGTGTCTGG AACTTGAGAA CAGCACCATT 
GAGTCAGTCA AGTTTGCGTC TGCCGAGAAC ATTCCAGACC TTCCCTACGA TTATAGCAGC 
AGTTTGGAGA GTGTTGCTGA TGAGACCTCC CCCGAAAGAG AAGGGAGGAG AGTCAACCTC 
ACGGGAAAGG CACCCAACAT CCTCCTCTAT GTGGGCTCCG ACTCCCAGGA AGCCCTCGGC 
CGGTTCCACG AGGTCCGGTC TGTGCTGGCC GACTGTGTGG ACATTGACAG TTATATTCTC 
TACCACCTGC TGGAGGACAG TGCTCTCAGA GACCCGTGGA CGGACAACTG TCTGCTGTTG 
GTCATTGCTA CCAGGGAGTC CATTCCCGAA GACCTGTACC AGAAGTTCAT GGCCTATCTT 
TCTCAGGGAG GGAAGGTGTT GGGCCTGTCT TCATCCTTCA CCTTTGGTGG CTTTCAGGTG 
ACAAGCAAGG GTGCACTGCA CAAGACAGTC CAGAACTTGG TTTTCTCCAA GGCTGACCAG 
AGCGAGGTGA AGCTCAGCGT CTTGAGCAGT GGCTGCAGGT ACCAGGAAGG CCCCGTCCGG 
CTCAGCCCCG GCAGGCTCCA GGGCCACCTG GAGAATGAGG ACAAGGACAG GATGATTGTG 
CATGTGCCTT TTGGAACTCG CGGGGGAGAA GCTGTTCTTT GCCAGGTGCA CTTAGAACTA 
CCTCCCAGCT CCAACATAGT GCAAACTCCA GAAGATTTTA ACTTGCTCAA GTCAAGCAAT 
TTTAGAAGAT ACGAAGTCCT TAGAGAGATT CTGACAACCC TTGGCCTCAG CTGTGACATG 
AAACAAGTTC CTGCCTTAAC TCCTCTTTAC TTGCTGTCAG CTGCGGAGGA AATCAGGGAT 
CCTCTTATGC AGTGGCTTGG GAAACATGTG GACTCCGAGG GAGAAATAAA ATCCGGCCAG 
CTCTCTCTTA GATTTGTTTC ATCCTACGTG TCTGAAGTAG AAATAACCCC ATCTTGTATA 
CCTGTGGTGA CCAACATGGA GGCCTTCTCA TCAGAACATT TCAACTTAGA GATCTATCGC 
CAAAATCTGC AGACCAAGCA GTTGGGGAAA GTAATTTTGT TTGCCGAAGT GACCCCCACA 
ACGATGCGTC TCCTGGATGG GCTGATGTTT CAGACACCGC AGGAAATGGG CTTAATAGTG 
ATCGCGGCCC GGCAGACCGA GGGCAAAGGA CGGGGAGGGA ATGTGTGGCT GAGCCCTGTG 
GGATGTGCTC TTTCTACTCT GCTCATCTCC ATTCCACTGA GATCCCAGCT GGGACAGAGG 
ATCCCGTTTG TCCAGCATCT GATGTCCGTG GCTGTCGTGG AAGCAGTGAG GTCCATTCCC 
GAGTATCAGG ATATCAACTT ACGAGTGAAG TGGCCCAACG ATATTTATTA CAGTGACCTC 
ATGAAGATCG GCGGAGTTCT GGTTAACTCA ACACTCATGG GAGAAACATT TTATATACTT 
ATTGGCTGTG GATTTAATGT GACTAACAGT AACCCTACCA TCTGCATCAA CGACCTCATC 
ACAGAATACA ATAAACAACA CAAGGCAGAA CTGAAGCCCT TAAGAGCCGA TTATCTCATC 
GCCAGAGTCG TGACTGTGCT GGAGAAACTG ATCAAAGAGT TTCAGGACAA AGGGCCCAAC 
AGCGTCCTTC CCCTTTATTA CCGATACTGG GTCCACAGTG GTCAGCAAGT CCATCTGGGC 
AGCGCAGAGG GACCAAAGGT GTCCATCGTT GGCCTGGACG ATTCTGGCTT CCTCCAGGTT 
CACCAGGAGG GCGGCGAGGT TGTGACTGTG CACCCGGACG GCAACTCCTT CGACATGCTG 
AGAAACCTCA TCCTCCCCAA ACGGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307834.1
CDS	418..2427
Translation	MEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	XM_024452066.1
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452066.1

ORF Insert Sequence:

ATGGAGCATG TTGGCAGAGA TGACCCAAAG GCTCTTGGTG AAGAACCCAA ACAAAGGAGA 
GGCAGTGCCT CTGGGAGTGA GCCTGCTGGG GACAGTGACA GGGGAGGGGG CCCCGTTGAG 
CATTATCACC TCCATCTGTC TAGTTGCCAC GAGTGTCTGG AACTTGAGAA CAGCACCATT 
GAGTCAGTCA AGTTTGCGTC TGCCGAGAAC ATTCCAGACC TTCCCTACGA TTATAGCAGC 
AGTTTGGAGA GTGTTGCTGA TGAGACCTCC CCCGAAAGAG AAGGGAGGAG AGTCAACCTC 
ACGGGAAAGG CACCCAACAT CCTCCTCTAT GTGGGCTCCG ACTCCCAGGA AGCCCTCGGC 
CGGTTCCACG AGGTCCGGTC TGTGCTGGCC GACTGTGTGG ACATTGACAG TTATATTCTC 
TACCACCTGC TGGAGGACAG TGCTCTCAGA GACCCGTGGA CGGACAACTG TCTGCTGTTG 
GTCATTGCTA CCAGGGAGTC CATTCCCGAA GACCTGTACC AGAAGTTCAT GGCCTATCTT 
TCTCAGGGAG GGAAGGTGTT GGGCCTGTCT TCATCCTTCA CCTTTGGTGG CTTTCAGGTG 
ACAAGCAAGG GTGCACTGCA CAAGACAGTC CAGAACTTGG TTTTCTCCAA GGCTGACCAG 
AGCGAGGTGA AGCTCAGCGT CTTGAGCAGT GGCTGCAGGT ACCAGGAAGG CCCCGTCCGG 
CTCAGCCCCG GCAGGCTCCA GGGCCACCTG GAGAATGAGG ACAAGGACAG GATGATTGTG 
CATGTGCCTT TTGGAACTCG CGGGGGAGAA GCTGTTCTTT GCCAGGTGCA CTTAGAACTA 
CCTCCCAGCT CCAACATAGT GCAAACTCCA GAAGATTTTA ACTTGCTCAA GTCAAGCAAT 
TTTAGAAGAT ACGAAGTCCT TAGAGAGATT CTGACAACCC TTGGCCTCAG CTGTGACATG 
AAACAAGTTC CTGCCTTAAC TCCTCTTTAC TTGCTGTCAG CTGCGGAGGA AATCAGGGAT 
CCTCTTATGC AGTGGCTTGG GAAACATGTG GACTCCGAGG GAGAAATAAA ATCCGGCCAG 
CTCTCTCTTA GATTTGTTTC ATCCTACGTG TCTGAAGTAG AAATAACCCC ATCTTGTATA 
CCTGTGGTGA CCAACATGGA GGCCTTCTCA TCAGAACATT TCAACTTAGA GATCTATCGC 
CAAAATCTGC AGACCAAGCA GTTGGGGAAA GTAATTTTGT TTGCCGAAGT GACCCCCACA 
ACGATGCGTC TCCTGGATGG GCTGATGTTT CAGACACCGC AGGAAATGGG CTTAATAGTG 
ATCGCGGCCC GGCAGACCGA GGGCAAAGGA CGGGGAGGGA ATGTGTGGCT GAGCCCTGTG 
GGATGTGCTC TTTCTACTCT GCTCATCTCC ATTCCACTGA GATCCCAGCT GGGACAGAGG 
ATCCCGTTTG TCCAGCATCT GATGTCCGTG GCTGTCGTGG AAGCAGTGAG GTCCATTCCC 
GAGTATCAGG ATATCAACTT ACGAGTGAAG TGGCCCAACG ATATTTATTA CAGTGACCTC 
ATGAAGATCG GCGGAGTTCT GGTTAACTCA ACACTCATGG GAGAAACATT TTATATACTT 
ATTGGCTGTG GATTTAATGT GACTAACAGT AACCCTACCA TCTGCATCAA CGACCTCATC 
ACAGAATACA ATAAACAACA CAAGGCAGAA CTGAAGCCCT TAAGAGCCGA TTATCTCATC 
GCCAGAGTCG TGACTGTGCT GGAGAAACTG ATCAAAGAGT TTCAGGACAA AGGGCCCAAC 
AGCGTCCTTC CCCTTTATTA CCGATACTGG GTCCACAGTG GTCAGCAAGT CCATCTGGGC 
AGCGCAGAGG GACCAAAGGT GTCCATCGTT GGCCTGGACG ATTCTGGCTT CCTCCAGGTT 
CACCAGGAGG GCGGCGAGGT TGTGACTGTG CACCCGGACG GCAACTCCTT CGACATGCTG 
AGAAACCTCA TCCTCCCCAA ACGGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_001352514.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339443.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 4, mRNA.

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CloneID	OHu53235
Clone ID Related Accession (Same CDS sequence)	XM_005260953.3 , NM_001352514.2 , NM_001352514.1
Accession Version	NM_001352514.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, AP000703.1, AP000702.1, AP000698.1 and AP000697.1. On Jun 2, 2019 this sequence version replaced NM_001352514.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.111587.1, SRR1803614.186134.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGTCGCCG GCCGGCTGAG 
CTCGTGCGCG CCACGGTGCG GCGGCTGCGT GCCTCGCGCT GTTCCTTCAC CTTCTGCGGC 
GCGGCCGCGC AGCCCCCGGG CGCCCGCGTG TGCCTGAGCC GTGGCGGCCG CGTCTTCTGC 
GTCAGCGACA GCCAGTCCAT TGAAGACTTG AACAAGTGGG CCCTATTTCT TGTGTCTCCT 
TTTATACTTG AAGCAGAACA CATAGCATTT GTGACGGAGA GCATTTGGGT ACAAAGTGAG 
AATTTACAGA GATCATCCTC TTCAGAAACA ATTGTCAAGT GGTCAGACTG TTGTTTGCCA 
TTAGCTTGCA GACCTGGGGA TCCTTATCGG CTAATTGCTG AAGCAAGTGT GGACAACTTC 
AGCAAGCTGG GGGTGGCGTT CATGGAAGAT AGACTCCACA TGGATAATGG ACTGGTACCC 
CAAAAGATTG TGTCGGTGCA CTTGCAGGAC TCCACTCTGA AGGAAGTTAA GGATCAGGTC 
TCAAACAAGC AAGCCCAGAT CCTAGAGCCG AAGCCTGAAC CTTCTCTTGA GATTAAGCCT 
GAGCAGGACG GTATGGAGCA TGTTGGCAGA GATGACCCAA AGGCTCTTGG TGAAGAACCC 
AAACAAAGGA GAGGCAGTGC CTCTGGGAGT GAGCCTGCTG GGGACAGTGA CAGGGGAGGG 
GGCCCCGTTG AGCATTATCA CCTCCATCTG TCTAGTTGCC ACGAGTGTCT GGAACTTGAG 
AACAGCACCA TTGAGTCAGT CAAGTTTGCG TCTGCCGAGA ACATTCCAGA CCTTCCCTAC 
GATTATAGCA GCAGTTTGGA GAGTGTTGCT GATGAGACCT CCCCCGAAAG AGAAGGGAGG 
AGAGTCAACC TCACGGGAAA GGCACCCAAC ATCCTCCTCT ATGTGGGCTC CGACTCCCAG 
GAAGCCCTCG GCCGGTTCCA CGAGGTCCGG TCTGTGCTGG CCGACTGTGT GGACATTGAC 
AGTTATATTC TCTACCACCT GCTGGAGGAC AGTGCTCTCA GAGACCCGTG GACGGACAAC 
TGTCTGCTGT TGGTCATTGC TACCAGGGAG TCCATTCCCG AAGACCTGTA CCAGAAGTTC 
ATGGCCTATC TTTCTCAGGG AGGGAAGGTG TTGGGCCTGT CTTCATCCTT CACCTTTGGT 
GGCTTTCAGG TGACAAGCAA GGGTGCACTG CACAAGACAG TCCAGAACTT GGTTTTCTCC 
AAGGCTGACC AGAGCGAGGT GAAGCTCAGC GTCTTGAGCA GTGGCTGCAG GTACCAGGAA 
GGCCCCGTCC GGCTCAGCCC CGGCAGGCTC CAGGGCCACC TGGAGAATGA GGACAAGGAC 
AGGATGATTG TGCATGTGCC TTTTGGAACT CGCGGGGGAG AAGCTGTTCT TTGCCAGGTG 
CACTTAGAAC TACCTCCCAG CTCCAACATA GTGCAAACTC CAGAAGATTT TAACTTGCTC 
AAGTCAAGCA ATTTTAGAAG ATACGAAGTC CTTAGAGAGA TTCTGACAAC CCTTGGCCTC 
AGCTGTGACA TGAAACAAGT TCCTGCCTTA ACTCCTCTTT ACTTGCTGTC AGCTGCGGAG 
GAAATCAGGG ATCCTCTTAT GCAGTGGCTT GGGAAACATG TGGACTCCGA GGGAGAAATA 
AAATCCGGCC AGCTCTCTCT TAGATTTGTT TCATCCTACG TGTCTGAAGT AGAAATAACC 
CCATCTTGTA TACCTGTGGT GACCAACATG GAGGCCTTCT CATCAGAACA TTTCAACTTA 
GAGATCTATC GCCAAAATCT GCAGACCAAG CAGTTGGGGA AAGTAATTTT GTTTGCCGAA 
GTGACCCCCA CAACGATGCG TCTCCTGGAT GGGCTGATGT TTCAGACACC GCAGGAAATG 
GGCTTAATAG TGATCGCGGC CCGGCAGACC GAGGGCAAAG GACGGGGAGG GAATGTGTGG 
CTGAGCCCTG TGGGATGTGC TCTTTCTACT CTGCTCATCT CCATTCCACT GAGATCCCAG 
CTGGGACAGA GGATCCCGTT TGTCCAGCAT CTGATGTCCG TGGCTGTCGT GGAAGCAGTG 
AGGTCCATTC CCGAGTATCA GGATATCAAC TTACGAGTGA AGTGGCCCAA CGATATTTAT 
TACAGTGACC TCATGAAGAT CGGCGGAGTT CTGGTTAACT CAACACTCAT GGGAGAAACA 
TTTTATATAC TTATTGGCTG TGGATTTAAT GTGACTAACA GTAACCCTAC CATCTGCATC 
AACGACCTCA TCACAGAATA CAATAAACAA CACAAGGCAG AACTGAAGCC CTTAAGAGCC 
GATTATCTCA TCGCCAGAGT CGTGACTGTG CTGGAGAAAC TGATCAAAGA GTTTCAGGAC 
AAAGGGCCCA ACAGCGTCCT TCCCCTTTAT TACCGATACT GGGTCCACAG TGGTCAGCAA 
GTCCATCTGG GCAGCGCAGA GGGACCAAAG GTGTCCATCG TTGGCCTGGA CGATTCTGGC 
TTCCTCCAGG TTCACCAGGA GGGCGGCGAG GTTGTGACTG TGCACCCGGA CGGCAACTCC 
TTCGACATGC TGAGAAACCT CATCCTCCCC AAACGGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339443.1
CDS	32..2653
Translation	MLITLCYLYLWARWGRRPAELVRATVRRLRASRCSFTFCGAAAQPPGARVCLSRGGRVFCVSDSQSIEDLNKWALFLVSPFILEAEHIAFVTESIWVQSENLQRSSSSETIVKWSDCCLPLACRPGDPYRLIAEASVDNFSKLGVAFMEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001352514.2
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352514.2

ORF Insert Sequence:

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGTCGCCG GCCGGCTGAG 
CTCGTGCGCG CCACGGTGCG GCGGCTGCGT GCCTCGCGCT GTTCCTTCAC CTTCTGCGGC 
GCGGCCGCGC AGCCCCCGGG CGCCCGCGTG TGCCTGAGCC GTGGCGGCCG CGTCTTCTGC 
GTCAGCGACA GCCAGTCCAT TGAAGACTTG AACAAGTGGG CCCTATTTCT TGTGTCTCCT 
TTTATACTTG AAGCAGAACA CATAGCATTT GTGACGGAGA GCATTTGGGT ACAAAGTGAG 
AATTTACAGA GATCATCCTC TTCAGAAACA ATTGTCAAGT GGTCAGACTG TTGTTTGCCA 
TTAGCTTGCA GACCTGGGGA TCCTTATCGG CTAATTGCTG AAGCAAGTGT GGACAACTTC 
AGCAAGCTGG GGGTGGCGTT CATGGAAGAT AGACTCCACA TGGATAATGG ACTGGTACCC 
CAAAAGATTG TGTCGGTGCA CTTGCAGGAC TCCACTCTGA AGGAAGTTAA GGATCAGGTC 
TCAAACAAGC AAGCCCAGAT CCTAGAGCCG AAGCCTGAAC CTTCTCTTGA GATTAAGCCT 
GAGCAGGACG GTATGGAGCA TGTTGGCAGA GATGACCCAA AGGCTCTTGG TGAAGAACCC 
AAACAAAGGA GAGGCAGTGC CTCTGGGAGT GAGCCTGCTG GGGACAGTGA CAGGGGAGGG 
GGCCCCGTTG AGCATTATCA CCTCCATCTG TCTAGTTGCC ACGAGTGTCT GGAACTTGAG 
AACAGCACCA TTGAGTCAGT CAAGTTTGCG TCTGCCGAGA ACATTCCAGA CCTTCCCTAC 
GATTATAGCA GCAGTTTGGA GAGTGTTGCT GATGAGACCT CCCCCGAAAG AGAAGGGAGG 
AGAGTCAACC TCACGGGAAA GGCACCCAAC ATCCTCCTCT ATGTGGGCTC CGACTCCCAG 
GAAGCCCTCG GCCGGTTCCA CGAGGTCCGG TCTGTGCTGG CCGACTGTGT GGACATTGAC 
AGTTATATTC TCTACCACCT GCTGGAGGAC AGTGCTCTCA GAGACCCGTG GACGGACAAC 
TGTCTGCTGT TGGTCATTGC TACCAGGGAG TCCATTCCCG AAGACCTGTA CCAGAAGTTC 
ATGGCCTATC TTTCTCAGGG AGGGAAGGTG TTGGGCCTGT CTTCATCCTT CACCTTTGGT 
GGCTTTCAGG TGACAAGCAA GGGTGCACTG CACAAGACAG TCCAGAACTT GGTTTTCTCC 
AAGGCTGACC AGAGCGAGGT GAAGCTCAGC GTCTTGAGCA GTGGCTGCAG GTACCAGGAA 
GGCCCCGTCC GGCTCAGCCC CGGCAGGCTC CAGGGCCACC TGGAGAATGA GGACAAGGAC 
AGGATGATTG TGCATGTGCC TTTTGGAACT CGCGGGGGAG AAGCTGTTCT TTGCCAGGTG 
CACTTAGAAC TACCTCCCAG CTCCAACATA GTGCAAACTC CAGAAGATTT TAACTTGCTC 
AAGTCAAGCA ATTTTAGAAG ATACGAAGTC CTTAGAGAGA TTCTGACAAC CCTTGGCCTC 
AGCTGTGACA TGAAACAAGT TCCTGCCTTA ACTCCTCTTT ACTTGCTGTC AGCTGCGGAG 
GAAATCAGGG ATCCTCTTAT GCAGTGGCTT GGGAAACATG TGGACTCCGA GGGAGAAATA 
AAATCCGGCC AGCTCTCTCT TAGATTTGTT TCATCCTACG TGTCTGAAGT AGAAATAACC 
CCATCTTGTA TACCTGTGGT GACCAACATG GAGGCCTTCT CATCAGAACA TTTCAACTTA 
GAGATCTATC GCCAAAATCT GCAGACCAAG CAGTTGGGGA AAGTAATTTT GTTTGCCGAA 
GTGACCCCCA CAACGATGCG TCTCCTGGAT GGGCTGATGT TTCAGACACC GCAGGAAATG 
GGCTTAATAG TGATCGCGGC CCGGCAGACC GAGGGCAAAG GACGGGGAGG GAATGTGTGG 
CTGAGCCCTG TGGGATGTGC TCTTTCTACT CTGCTCATCT CCATTCCACT GAGATCCCAG 
CTGGGACAGA GGATCCCGTT TGTCCAGCAT CTGATGTCCG TGGCTGTCGT GGAAGCAGTG 
AGGTCCATTC CCGAGTATCA GGATATCAAC TTACGAGTGA AGTGGCCCAA CGATATTTAT 
TACAGTGACC TCATGAAGAT CGGCGGAGTT CTGGTTAACT CAACACTCAT GGGAGAAACA 
TTTTATATAC TTATTGGCTG TGGATTTAAT GTGACTAACA GTAACCCTAC CATCTGCATC 
AACGACCTCA TCACAGAATA CAATAAACAA CACAAGGCAG AACTGAAGCC CTTAAGAGCC 
GATTATCTCA TCGCCAGAGT CGTGACTGTG CTGGAGAAAC TGATCAAAGA GTTTCAGGAC 
AAAGGGCCCA ACAGCGTCCT TCCCCTTTAT TACCGATACT GGGTCCACAG TGGTCAGCAA 
GTCCATCTGG GCAGCGCAGA GGGACCAAAG GTGTCCATCG TTGGCCTGGA CGATTCTGGC 
TTCCTCCAGG TTCACCAGGA GGGCGGCGAG GTTGTGACTG TGCACCCGGA CGGCAACTCC 
TTCGACATGC TGAGAAACCT CATCCTCCCC AAACGGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( NM_001352514.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339443.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu53235
Clone ID Related Accession (Same CDS sequence)	XM_005260953.3 , NM_001352514.2 , NM_001352514.1
Accession Version	NM_001352514.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000704.2, AP000703.1, AP000702.1, AP000698.1 and AP000697.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.111587.1, SRR1803614.186134.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGTCGCCG GCCGGCTGAG 
CTCGTGCGCG CCACGGTGCG GCGGCTGCGT GCCTCGCGCT GTTCCTTCAC CTTCTGCGGC 
GCGGCCGCGC AGCCCCCGGG CGCCCGCGTG TGCCTGAGCC GTGGCGGCCG CGTCTTCTGC 
GTCAGCGACA GCCAGTCCAT TGAAGACTTG AACAAGTGGG CCCTATTTCT TGTGTCTCCT 
TTTATACTTG AAGCAGAACA CATAGCATTT GTGACGGAGA GCATTTGGGT ACAAAGTGAG 
AATTTACAGA GATCATCCTC TTCAGAAACA ATTGTCAAGT GGTCAGACTG TTGTTTGCCA 
TTAGCTTGCA GACCTGGGGA TCCTTATCGG CTAATTGCTG AAGCAAGTGT GGACAACTTC 
AGCAAGCTGG GGGTGGCGTT CATGGAAGAT AGACTCCACA TGGATAATGG ACTGGTACCC 
CAAAAGATTG TGTCGGTGCA CTTGCAGGAC TCCACTCTGA AGGAAGTTAA GGATCAGGTC 
TCAAACAAGC AAGCCCAGAT CCTAGAGCCG AAGCCTGAAC CTTCTCTTGA GATTAAGCCT 
GAGCAGGACG GTATGGAGCA TGTTGGCAGA GATGACCCAA AGGCTCTTGG TGAAGAACCC 
AAACAAAGGA GAGGCAGTGC CTCTGGGAGT GAGCCTGCTG GGGACAGTGA CAGGGGAGGG 
GGCCCCGTTG AGCATTATCA CCTCCATCTG TCTAGTTGCC ACGAGTGTCT GGAACTTGAG 
AACAGCACCA TTGAGTCAGT CAAGTTTGCG TCTGCCGAGA ACATTCCAGA CCTTCCCTAC 
GATTATAGCA GCAGTTTGGA GAGTGTTGCT GATGAGACCT CCCCCGAAAG AGAAGGGAGG 
AGAGTCAACC TCACGGGAAA GGCACCCAAC ATCCTCCTCT ATGTGGGCTC CGACTCCCAG 
GAAGCCCTCG GCCGGTTCCA CGAGGTCCGG TCTGTGCTGG CCGACTGTGT GGACATTGAC 
AGTTATATTC TCTACCACCT GCTGGAGGAC AGTGCTCTCA GAGACCCGTG GACGGACAAC 
TGTCTGCTGT TGGTCATTGC TACCAGGGAG TCCATTCCCG AAGACCTGTA CCAGAAGTTC 
ATGGCCTATC TTTCTCAGGG AGGGAAGGTG TTGGGCCTGT CTTCATCCTT CACCTTTGGT 
GGCTTTCAGG TGACAAGCAA GGGTGCACTG CACAAGACAG TCCAGAACTT GGTTTTCTCC 
AAGGCTGACC AGAGCGAGGT GAAGCTCAGC GTCTTGAGCA GTGGCTGCAG GTACCAGGAA 
GGCCCCGTCC GGCTCAGCCC CGGCAGGCTC CAGGGCCACC TGGAGAATGA GGACAAGGAC 
AGGATGATTG TGCATGTGCC TTTTGGAACT CGCGGGGGAG AAGCTGTTCT TTGCCAGGTG 
CACTTAGAAC TACCTCCCAG CTCCAACATA GTGCAAACTC CAGAAGATTT TAACTTGCTC 
AAGTCAAGCA ATTTTAGAAG ATACGAAGTC CTTAGAGAGA TTCTGACAAC CCTTGGCCTC 
AGCTGTGACA TGAAACAAGT TCCTGCCTTA ACTCCTCTTT ACTTGCTGTC AGCTGCGGAG 
GAAATCAGGG ATCCTCTTAT GCAGTGGCTT GGGAAACATG TGGACTCCGA GGGAGAAATA 
AAATCCGGCC AGCTCTCTCT TAGATTTGTT TCATCCTACG TGTCTGAAGT AGAAATAACC 
CCATCTTGTA TACCTGTGGT GACCAACATG GAGGCCTTCT CATCAGAACA TTTCAACTTA 
GAGATCTATC GCCAAAATCT GCAGACCAAG CAGTTGGGGA AAGTAATTTT GTTTGCCGAA 
GTGACCCCCA CAACGATGCG TCTCCTGGAT GGGCTGATGT TTCAGACACC GCAGGAAATG 
GGCTTAATAG TGATCGCGGC CCGGCAGACC GAGGGCAAAG GACGGGGAGG GAATGTGTGG 
CTGAGCCCTG TGGGATGTGC TCTTTCTACT CTGCTCATCT CCATTCCACT GAGATCCCAG 
CTGGGACAGA GGATCCCGTT TGTCCAGCAT CTGATGTCCG TGGCTGTCGT GGAAGCAGTG 
AGGTCCATTC CCGAGTATCA GGATATCAAC TTACGAGTGA AGTGGCCCAA CGATATTTAT 
TACAGTGACC TCATGAAGAT CGGCGGAGTT CTGGTTAACT CAACACTCAT GGGAGAAACA 
TTTTATATAC TTATTGGCTG TGGATTTAAT GTGACTAACA GTAACCCTAC CATCTGCATC 
AACGACCTCA TCACAGAATA CAATAAACAA CACAAGGCAG AACTGAAGCC CTTAAGAGCC 
GATTATCTCA TCGCCAGAGT CGTGACTGTG CTGGAGAAAC TGATCAAAGA GTTTCAGGAC 
AAAGGGCCCA ACAGCGTCCT TCCCCTTTAT TACCGATACT GGGTCCACAG TGGTCAGCAA 
GTCCATCTGG GCAGCGCAGA GGGACCAAAG GTGTCCATCG TTGGCCTGGA CGATTCTGGC 
TTCCTCCAGG TTCACCAGGA GGGCGGCGAG GTTGTGACTG TGCACCCGGA CGGCAACTCC 
TTCGACATGC TGAGAAACCT CATCCTCCCC AAACGGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339443.1
CDS	53..2674
Translation	MLITLCYLYLWARWGRRPAELVRATVRRLRASRCSFTFCGAAAQPPGARVCLSRGGRVFCVSDSQSIEDLNKWALFLVSPFILEAEHIAFVTESIWVQSENLQRSSSSETIVKWSDCCLPLACRPGDPYRLIAEASVDNFSKLGVAFMEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	NM_001352514.1
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352514.1

ORF Insert Sequence:

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGTCGCCG GCCGGCTGAG 
CTCGTGCGCG CCACGGTGCG GCGGCTGCGT GCCTCGCGCT GTTCCTTCAC CTTCTGCGGC 
GCGGCCGCGC AGCCCCCGGG CGCCCGCGTG TGCCTGAGCC GTGGCGGCCG CGTCTTCTGC 
GTCAGCGACA GCCAGTCCAT TGAAGACTTG AACAAGTGGG CCCTATTTCT TGTGTCTCCT 
TTTATACTTG AAGCAGAACA CATAGCATTT GTGACGGAGA GCATTTGGGT ACAAAGTGAG 
AATTTACAGA GATCATCCTC TTCAGAAACA ATTGTCAAGT GGTCAGACTG TTGTTTGCCA 
TTAGCTTGCA GACCTGGGGA TCCTTATCGG CTAATTGCTG AAGCAAGTGT GGACAACTTC 
AGCAAGCTGG GGGTGGCGTT CATGGAAGAT AGACTCCACA TGGATAATGG ACTGGTACCC 
CAAAAGATTG TGTCGGTGCA CTTGCAGGAC TCCACTCTGA AGGAAGTTAA GGATCAGGTC 
TCAAACAAGC AAGCCCAGAT CCTAGAGCCG AAGCCTGAAC CTTCTCTTGA GATTAAGCCT 
GAGCAGGACG GTATGGAGCA TGTTGGCAGA GATGACCCAA AGGCTCTTGG TGAAGAACCC 
AAACAAAGGA GAGGCAGTGC CTCTGGGAGT GAGCCTGCTG GGGACAGTGA CAGGGGAGGG 
GGCCCCGTTG AGCATTATCA CCTCCATCTG TCTAGTTGCC ACGAGTGTCT GGAACTTGAG 
AACAGCACCA TTGAGTCAGT CAAGTTTGCG TCTGCCGAGA ACATTCCAGA CCTTCCCTAC 
GATTATAGCA GCAGTTTGGA GAGTGTTGCT GATGAGACCT CCCCCGAAAG AGAAGGGAGG 
AGAGTCAACC TCACGGGAAA GGCACCCAAC ATCCTCCTCT ATGTGGGCTC CGACTCCCAG 
GAAGCCCTCG GCCGGTTCCA CGAGGTCCGG TCTGTGCTGG CCGACTGTGT GGACATTGAC 
AGTTATATTC TCTACCACCT GCTGGAGGAC AGTGCTCTCA GAGACCCGTG GACGGACAAC 
TGTCTGCTGT TGGTCATTGC TACCAGGGAG TCCATTCCCG AAGACCTGTA CCAGAAGTTC 
ATGGCCTATC TTTCTCAGGG AGGGAAGGTG TTGGGCCTGT CTTCATCCTT CACCTTTGGT 
GGCTTTCAGG TGACAAGCAA GGGTGCACTG CACAAGACAG TCCAGAACTT GGTTTTCTCC 
AAGGCTGACC AGAGCGAGGT GAAGCTCAGC GTCTTGAGCA GTGGCTGCAG GTACCAGGAA 
GGCCCCGTCC GGCTCAGCCC CGGCAGGCTC CAGGGCCACC TGGAGAATGA GGACAAGGAC 
AGGATGATTG TGCATGTGCC TTTTGGAACT CGCGGGGGAG AAGCTGTTCT TTGCCAGGTG 
CACTTAGAAC TACCTCCCAG CTCCAACATA GTGCAAACTC CAGAAGATTT TAACTTGCTC 
AAGTCAAGCA ATTTTAGAAG ATACGAAGTC CTTAGAGAGA TTCTGACAAC CCTTGGCCTC 
AGCTGTGACA TGAAACAAGT TCCTGCCTTA ACTCCTCTTT ACTTGCTGTC AGCTGCGGAG 
GAAATCAGGG ATCCTCTTAT GCAGTGGCTT GGGAAACATG TGGACTCCGA GGGAGAAATA 
AAATCCGGCC AGCTCTCTCT TAGATTTGTT TCATCCTACG TGTCTGAAGT AGAAATAACC 
CCATCTTGTA TACCTGTGGT GACCAACATG GAGGCCTTCT CATCAGAACA TTTCAACTTA 
GAGATCTATC GCCAAAATCT GCAGACCAAG CAGTTGGGGA AAGTAATTTT GTTTGCCGAA 
GTGACCCCCA CAACGATGCG TCTCCTGGAT GGGCTGATGT TTCAGACACC GCAGGAAATG 
GGCTTAATAG TGATCGCGGC CCGGCAGACC GAGGGCAAAG GACGGGGAGG GAATGTGTGG 
CTGAGCCCTG TGGGATGTGC TCTTTCTACT CTGCTCATCT CCATTCCACT GAGATCCCAG 
CTGGGACAGA GGATCCCGTT TGTCCAGCAT CTGATGTCCG TGGCTGTCGT GGAAGCAGTG 
AGGTCCATTC CCGAGTATCA GGATATCAAC TTACGAGTGA AGTGGCCCAA CGATATTTAT 
TACAGTGACC TCATGAAGAT CGGCGGAGTT CTGGTTAACT CAACACTCAT GGGAGAAACA 
TTTTATATAC TTATTGGCTG TGGATTTAAT GTGACTAACA GTAACCCTAC CATCTGCATC 
AACGACCTCA TCACAGAATA CAATAAACAA CACAAGGCAG AACTGAAGCC CTTAAGAGCC 
GATTATCTCA TCGCCAGAGT CGTGACTGTG CTGGAGAAAC TGATCAAAGA GTTTCAGGAC 
AAAGGGCCCA ACAGCGTCCT TCCCCTTTAT TACCGATACT GGGTCCACAG TGGTCAGCAA 
GTCCATCTGG GCAGCGCAGA GGGACCAAAG GTGTCCATCG TTGGCCTGGA CGATTCTGGC 
TTCCTCCAGG TTCACCAGGA GGGCGGCGAG GTTGTGACTG TGCACCCGGA CGGCAACTCC 
TTCGACATGC TGAGAAACCT CATCCTCCCC AAACGGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_005260953.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005261010.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

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CloneID	OHu53235
Clone ID Related Accession (Same CDS sequence)	XM_005260953.3 , NM_001352514.2 , NM_001352514.1
Accession Version	XM_005260953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260953.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGTCGCCG GCCGGCTGAG 
CTCGTGCGCG CCACGGTGCG GCGGCTGCGT GCCTCGCGCT GTTCCTTCAC CTTCTGCGGC 
GCGGCCGCGC AGCCCCCGGG CGCCCGCGTG TGCCTGAGCC GTGGCGGCCG CGTCTTCTGC 
GTCAGCGACA GCCAGTCCAT TGAAGACTTG AACAAGTGGG CCCTATTTCT TGTGTCTCCT 
TTTATACTTG AAGCAGAACA CATAGCATTT GTGACGGAGA GCATTTGGGT ACAAAGTGAG 
AATTTACAGA GATCATCCTC TTCAGAAACA ATTGTCAAGT GGTCAGACTG TTGTTTGCCA 
TTAGCTTGCA GACCTGGGGA TCCTTATCGG CTAATTGCTG AAGCAAGTGT GGACAACTTC 
AGCAAGCTGG GGGTGGCGTT CATGGAAGAT AGACTCCACA TGGATAATGG ACTGGTACCC 
CAAAAGATTG TGTCGGTGCA CTTGCAGGAC TCCACTCTGA AGGAAGTTAA GGATCAGGTC 
TCAAACAAGC AAGCCCAGAT CCTAGAGCCG AAGCCTGAAC CTTCTCTTGA GATTAAGCCT 
GAGCAGGACG GTATGGAGCA TGTTGGCAGA GATGACCCAA AGGCTCTTGG TGAAGAACCC 
AAACAAAGGA GAGGCAGTGC CTCTGGGAGT GAGCCTGCTG GGGACAGTGA CAGGGGAGGG 
GGCCCCGTTG AGCATTATCA CCTCCATCTG TCTAGTTGCC ACGAGTGTCT GGAACTTGAG 
AACAGCACCA TTGAGTCAGT CAAGTTTGCG TCTGCCGAGA ACATTCCAGA CCTTCCCTAC 
GATTATAGCA GCAGTTTGGA GAGTGTTGCT GATGAGACCT CCCCCGAAAG AGAAGGGAGG 
AGAGTCAACC TCACGGGAAA GGCACCCAAC ATCCTCCTCT ATGTGGGCTC CGACTCCCAG 
GAAGCCCTCG GCCGGTTCCA CGAGGTCCGG TCTGTGCTGG CCGACTGTGT GGACATTGAC 
AGTTATATTC TCTACCACCT GCTGGAGGAC AGTGCTCTCA GAGACCCGTG GACGGACAAC 
TGTCTGCTGT TGGTCATTGC TACCAGGGAG TCCATTCCCG AAGACCTGTA CCAGAAGTTC 
ATGGCCTATC TTTCTCAGGG AGGGAAGGTG TTGGGCCTGT CTTCATCCTT CACCTTTGGT 
GGCTTTCAGG TGACAAGCAA GGGTGCACTG CACAAGACAG TCCAGAACTT GGTTTTCTCC 
AAGGCTGACC AGAGCGAGGT GAAGCTCAGC GTCTTGAGCA GTGGCTGCAG GTACCAGGAA 
GGCCCCGTCC GGCTCAGCCC CGGCAGGCTC CAGGGCCACC TGGAGAATGA GGACAAGGAC 
AGGATGATTG TGCATGTGCC TTTTGGAACT CGCGGGGGAG AAGCTGTTCT TTGCCAGGTG 
CACTTAGAAC TACCTCCCAG CTCCAACATA GTGCAAACTC CAGAAGATTT TAACTTGCTC 
AAGTCAAGCA ATTTTAGAAG ATACGAAGTC CTTAGAGAGA TTCTGACAAC CCTTGGCCTC 
AGCTGTGACA TGAAACAAGT TCCTGCCTTA ACTCCTCTTT ACTTGCTGTC AGCTGCGGAG 
GAAATCAGGG ATCCTCTTAT GCAGTGGCTT GGGAAACATG TGGACTCCGA GGGAGAAATA 
AAATCCGGCC AGCTCTCTCT TAGATTTGTT TCATCCTACG TGTCTGAAGT AGAAATAACC 
CCATCTTGTA TACCTGTGGT GACCAACATG GAGGCCTTCT CATCAGAACA TTTCAACTTA 
GAGATCTATC GCCAAAATCT GCAGACCAAG CAGTTGGGGA AAGTAATTTT GTTTGCCGAA 
GTGACCCCCA CAACGATGCG TCTCCTGGAT GGGCTGATGT TTCAGACACC GCAGGAAATG 
GGCTTAATAG TGATCGCGGC CCGGCAGACC GAGGGCAAAG GACGGGGAGG GAATGTGTGG 
CTGAGCCCTG TGGGATGTGC TCTTTCTACT CTGCTCATCT CCATTCCACT GAGATCCCAG 
CTGGGACAGA GGATCCCGTT TGTCCAGCAT CTGATGTCCG TGGCTGTCGT GGAAGCAGTG 
AGGTCCATTC CCGAGTATCA GGATATCAAC TTACGAGTGA AGTGGCCCAA CGATATTTAT 
TACAGTGACC TCATGAAGAT CGGCGGAGTT CTGGTTAACT CAACACTCAT GGGAGAAACA 
TTTTATATAC TTATTGGCTG TGGATTTAAT GTGACTAACA GTAACCCTAC CATCTGCATC 
AACGACCTCA TCACAGAATA CAATAAACAA CACAAGGCAG AACTGAAGCC CTTAAGAGCC 
GATTATCTCA TCGCCAGAGT CGTGACTGTG CTGGAGAAAC TGATCAAAGA GTTTCAGGAC 
AAAGGGCCCA ACAGCGTCCT TCCCCTTTAT TACCGATACT GGGTCCACAG TGGTCAGCAA 
GTCCATCTGG GCAGCGCAGA GGGACCAAAG GTGTCCATCG TTGGCCTGGA CGATTCTGGC 
TTCCTCCAGG TTCACCAGGA GGGCGGCGAG GTTGTGACTG TGCACCCGGA CGGCAACTCC 
TTCGACATGC TGAGAAACCT CATCCTCCCC AAACGGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005261010.1
CDS	2..2623
Translation	MLITLCYLYLWARWGRRPAELVRATVRRLRASRCSFTFCGAAAQPPGARVCLSRGGRVFCVSDSQSIEDLNKWALFLVSPFILEAEHIAFVTESIWVQSENLQRSSSSETIVKWSDCCLPLACRPGDPYRLIAEASVDNFSKLGVAFMEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

Target ORF information:

RefSeq Version	XM_005260953.3
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260953.3

ORF Insert Sequence:

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGTCGCCG GCCGGCTGAG 
CTCGTGCGCG CCACGGTGCG GCGGCTGCGT GCCTCGCGCT GTTCCTTCAC CTTCTGCGGC 
GCGGCCGCGC AGCCCCCGGG CGCCCGCGTG TGCCTGAGCC GTGGCGGCCG CGTCTTCTGC 
GTCAGCGACA GCCAGTCCAT TGAAGACTTG AACAAGTGGG CCCTATTTCT TGTGTCTCCT 
TTTATACTTG AAGCAGAACA CATAGCATTT GTGACGGAGA GCATTTGGGT ACAAAGTGAG 
AATTTACAGA GATCATCCTC TTCAGAAACA ATTGTCAAGT GGTCAGACTG TTGTTTGCCA 
TTAGCTTGCA GACCTGGGGA TCCTTATCGG CTAATTGCTG AAGCAAGTGT GGACAACTTC 
AGCAAGCTGG GGGTGGCGTT CATGGAAGAT AGACTCCACA TGGATAATGG ACTGGTACCC 
CAAAAGATTG TGTCGGTGCA CTTGCAGGAC TCCACTCTGA AGGAAGTTAA GGATCAGGTC 
TCAAACAAGC AAGCCCAGAT CCTAGAGCCG AAGCCTGAAC CTTCTCTTGA GATTAAGCCT 
GAGCAGGACG GTATGGAGCA TGTTGGCAGA GATGACCCAA AGGCTCTTGG TGAAGAACCC 
AAACAAAGGA GAGGCAGTGC CTCTGGGAGT GAGCCTGCTG GGGACAGTGA CAGGGGAGGG 
GGCCCCGTTG AGCATTATCA CCTCCATCTG TCTAGTTGCC ACGAGTGTCT GGAACTTGAG 
AACAGCACCA TTGAGTCAGT CAAGTTTGCG TCTGCCGAGA ACATTCCAGA CCTTCCCTAC 
GATTATAGCA GCAGTTTGGA GAGTGTTGCT GATGAGACCT CCCCCGAAAG AGAAGGGAGG 
AGAGTCAACC TCACGGGAAA GGCACCCAAC ATCCTCCTCT ATGTGGGCTC CGACTCCCAG 
GAAGCCCTCG GCCGGTTCCA CGAGGTCCGG TCTGTGCTGG CCGACTGTGT GGACATTGAC 
AGTTATATTC TCTACCACCT GCTGGAGGAC AGTGCTCTCA GAGACCCGTG GACGGACAAC 
TGTCTGCTGT TGGTCATTGC TACCAGGGAG TCCATTCCCG AAGACCTGTA CCAGAAGTTC 
ATGGCCTATC TTTCTCAGGG AGGGAAGGTG TTGGGCCTGT CTTCATCCTT CACCTTTGGT 
GGCTTTCAGG TGACAAGCAA GGGTGCACTG CACAAGACAG TCCAGAACTT GGTTTTCTCC 
AAGGCTGACC AGAGCGAGGT GAAGCTCAGC GTCTTGAGCA GTGGCTGCAG GTACCAGGAA 
GGCCCCGTCC GGCTCAGCCC CGGCAGGCTC CAGGGCCACC TGGAGAATGA GGACAAGGAC 
AGGATGATTG TGCATGTGCC TTTTGGAACT CGCGGGGGAG AAGCTGTTCT TTGCCAGGTG 
CACTTAGAAC TACCTCCCAG CTCCAACATA GTGCAAACTC CAGAAGATTT TAACTTGCTC 
AAGTCAAGCA ATTTTAGAAG ATACGAAGTC CTTAGAGAGA TTCTGACAAC CCTTGGCCTC 
AGCTGTGACA TGAAACAAGT TCCTGCCTTA ACTCCTCTTT ACTTGCTGTC AGCTGCGGAG 
GAAATCAGGG ATCCTCTTAT GCAGTGGCTT GGGAAACATG TGGACTCCGA GGGAGAAATA 
AAATCCGGCC AGCTCTCTCT TAGATTTGTT TCATCCTACG TGTCTGAAGT AGAAATAACC 
CCATCTTGTA TACCTGTGGT GACCAACATG GAGGCCTTCT CATCAGAACA TTTCAACTTA 
GAGATCTATC GCCAAAATCT GCAGACCAAG CAGTTGGGGA AAGTAATTTT GTTTGCCGAA 
GTGACCCCCA CAACGATGCG TCTCCTGGAT GGGCTGATGT TTCAGACACC GCAGGAAATG 
GGCTTAATAG TGATCGCGGC CCGGCAGACC GAGGGCAAAG GACGGGGAGG GAATGTGTGG 
CTGAGCCCTG TGGGATGTGC TCTTTCTACT CTGCTCATCT CCATTCCACT GAGATCCCAG 
CTGGGACAGA GGATCCCGTT TGTCCAGCAT CTGATGTCCG TGGCTGTCGT GGAAGCAGTG 
AGGTCCATTC CCGAGTATCA GGATATCAAC TTACGAGTGA AGTGGCCCAA CGATATTTAT 
TACAGTGACC TCATGAAGAT CGGCGGAGTT CTGGTTAACT CAACACTCAT GGGAGAAACA 
TTTTATATAC TTATTGGCTG TGGATTTAAT GTGACTAACA GTAACCCTAC CATCTGCATC 
AACGACCTCA TCACAGAATA CAATAAACAA CACAAGGCAG AACTGAAGCC CTTAAGAGCC 
GATTATCTCA TCGCCAGAGT CGTGACTGTG CTGGAGAAAC TGATCAAAGA GTTTCAGGAC 
AAAGGGCCCA ACAGCGTCCT TCCCCTTTAT TACCGATACT GGGTCCACAG TGGTCAGCAA 
GTCCATCTGG GCAGCGCAGA GGGACCAAAG GTGTCCATCG TTGGCCTGGA CGATTCTGGC 
TTCCTCCAGG TTCACCAGGA GGGCGGCGAG GTTGTGACTG TGCACCCGGA CGGCAACTCC 
TTCGACATGC TGAGAAACCT CATCCTCCCC AAACGGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_011529540.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527842.1 Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X10, mRNA.

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CloneID	OHu58262
Clone ID Related Accession (Same CDS sequence)	XM_011529540.2
Accession Version	XM_011529540.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	biotin--protein ligase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGTCGCCG GCCGGCTGAG 
CTCGTGCGCG CCACGGTGCG GCGGCTGCGT GCCTCGCGCT GTTCCTTCAC CTTCTGCGGC 
GCGGCCGCGC AGCCCCCGGG CGCCCGCGTG TGCCTGAGCC GTGGCGGCCG CGTCTTCTGC 
GTCAGCGACA GCCAGTCCAT TGAAGACTTG AACAAGTGGG CCCTATTTCT TGTGTCTCCT 
TTTATACTTG AAGCAGAACA CATAGCATTT GTGACGGAGA GCATTTGGGT ACAAAGTGAG 
AATTTACAGA GATCATCCTC TTCAGAAACA ATTGTCAAGT GGTCAGACTG TTGTTTGCCA 
TTAGCTTGCA GACCTGGGGA TCCTTATCGG CTAATTGCTG AAGCAAGTGT GGACAACTTC 
AGCAAGCTGG GGGTGGCGTT CATGGAAGAT AGACTCCACA TGGATAATGG ACTGGTACCC 
CAAAAGATTG TGTCGGTGCA CTTGCAGGAC TCCACTCTGA AGGAAGTTAA GGATCAGGTC 
TCAAACAAGC AAGCCCAGAT CCTAGAGCCG AAGCCTGAAC CTTCTCTTGA GATTAAGCCT 
GAGCAGGACG GTATGGAGCA TGTTGGCAGA GATGACCCAA AGGCTCTTGG TGAAGAACCC 
AAACAAAGGA GAGGCAGTGC CTCTGGGAGT GAGCCTGCTG GGGACAGTGA CAGGGGAGGG 
GGCCCCGTTG AGCATTATCA CCTCCATCTG TCTAGTTGCC ACGAGTGTCT GGAACTTGAG 
AACAGCACCA TTGAGTCAGT CAAGTTTGCG TCTGCCGAGA ACATTCCAGA CCTTCCCTAC 
GATTATAGCA GCAGTTTGGA GAGTGTTGCT GATGAGACCT CCCCCGAAAG AGAAGGGAGG 
AGAGTCAACC TCACGGGAAA GGCACCCAAC ATCCTCCTCT ATGTGGGCTC CGACTCCCAG 
GAAGCCCTCG GCCGGTTCCA CGAGGTCCGG TCTGTGCTGG CCGACTGTGT GGACATTGAC 
AGTTATATTC TCTACCACCT GCTGGAGGAC AGTGCTCTCA GAGACCCGTG GACGGACAAC 
TGTCTGCTGT TGGTCATTGC TACCAGGGAG TCCATTCCCG AAGACCTGTA CCAGAAGTTC 
ATGGCCTATC TTTCTCAGGG AGGGAAGGTG TTGGGCCTGT CTTCATCCTT CACCTTTGGT 
GGCTTTCAGG TGACAAGCAA GGGTGCACTG CACAAGACAG TCCAGAACTT GGTTTTCTCC 
AAGGCTGACC AGAGCGAGGT GAAGCTCAGC GTCTTGAGCA GTGGCTGCAG GTACCAGGAA 
GGCCCCGTCC GGCTCAGCCC CGGCAGGCTC CAGGGCCACC TGGAGAATGA GGACAAGGAC 
AGGATGATTG TGCATGTGCC TTTTGGAACT CGCGGGGGAG AAGCTGTTCT TTGCCAGGTG 
CACTTAGAAC TACCTCCCAG CTCCAACATA GTGCAAACTC CAGAAGATTT TAACTTGCTC 
AAGTCAAGCA ATTTTAGAAG ATACGAAGTC CTTAGAGAGA TTCTGACAAC CCTTGGCCTC 
AGCTGTGACA TGAAACAAGT TCCTGCCTTA ACTCCTCTTT ACTTGCTGTC AGCTGCGGAG 
GAAATCAGGG ATCCTCTTAT GCAGTGGCTT GGGAAACATG TGGACTCCGA GGGAGAAATA 
AAATCCGGCC AGCTCTCTCT TAGATTTGTT TCATCCTACG TGTCTGAAGT AGAAATAACC 
CCATCTTGTA TACCTGTGGT GACCAACATG GAGGCCTTCT CATCAGAACA TTTCAACTTA 
GAGATCTATC GCCAAAATCT GCAGACCAAG CAGTTGGGGA AAGTAATTTT GTTTGCCGAA 
GTGACCCCCA CAACGATGCG TCTCCTGGAT GGGGCCGGAT GTACCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527842.1
CDS	2..1912
Translation	MLITLCYLYLWARWGRRPAELVRATVRRLRASRCSFTFCGAAAQPPGARVCLSRGGRVFCVSDSQSIEDLNKWALFLVSPFILEAEHIAFVTESIWVQSENLQRSSSSETIVKWSDCCLPLACRPGDPYRLIAEASVDNFSKLGVAFMEDRLHMDNGLVPQKIVSVHLQDSTLKEVKDQVSNKQAQILEPKPEPSLEIKPEQDGMEHVGRDDPKALGEEPKQRRGSASGSEPAGDSDRGGGPVEHYHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQVTSKGALHKTVQNLVFSKADQSEVKLSVLSSGCRYQEGPVRLSPGRLQGHLENEDKDRMIVHVPFGTRGGEAVLCQVHLELPPSSNIVQTPEDFNLLKSSNFRRYEVLREILTTLGLSCDMKQVPALTPLYLLSAAEEIRDPLMQWLGKHVDSEGEIKSGQLSLRFVSSYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGAGCTC

Target ORF information:

RefSeq Version	XM_011529540.2
Organism	Homo sapiens(human)
Definition	Homo sapiens holocarboxylase synthetase (HLCS), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529540.2

ORF Insert Sequence:

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGTCGCCG GCCGGCTGAG 
CTCGTGCGCG CCACGGTGCG GCGGCTGCGT GCCTCGCGCT GTTCCTTCAC CTTCTGCGGC 
GCGGCCGCGC AGCCCCCGGG CGCCCGCGTG TGCCTGAGCC GTGGCGGCCG CGTCTTCTGC 
GTCAGCGACA GCCAGTCCAT TGAAGACTTG AACAAGTGGG CCCTATTTCT TGTGTCTCCT 
TTTATACTTG AAGCAGAACA CATAGCATTT GTGACGGAGA GCATTTGGGT ACAAAGTGAG 
AATTTACAGA GATCATCCTC TTCAGAAACA ATTGTCAAGT GGTCAGACTG TTGTTTGCCA 
TTAGCTTGCA GACCTGGGGA TCCTTATCGG CTAATTGCTG AAGCAAGTGT GGACAACTTC 
AGCAAGCTGG GGGTGGCGTT CATGGAAGAT AGACTCCACA TGGATAATGG ACTGGTACCC 
CAAAAGATTG TGTCGGTGCA CTTGCAGGAC TCCACTCTGA AGGAAGTTAA GGATCAGGTC 
TCAAACAAGC AAGCCCAGAT CCTAGAGCCG AAGCCTGAAC CTTCTCTTGA GATTAAGCCT 
GAGCAGGACG GTATGGAGCA TGTTGGCAGA GATGACCCAA AGGCTCTTGG TGAAGAACCC 
AAACAAAGGA GAGGCAGTGC CTCTGGGAGT GAGCCTGCTG GGGACAGTGA CAGGGGAGGG 
GGCCCCGTTG AGCATTATCA CCTCCATCTG TCTAGTTGCC ACGAGTGTCT GGAACTTGAG 
AACAGCACCA TTGAGTCAGT CAAGTTTGCG TCTGCCGAGA ACATTCCAGA CCTTCCCTAC 
GATTATAGCA GCAGTTTGGA GAGTGTTGCT GATGAGACCT CCCCCGAAAG AGAAGGGAGG 
AGAGTCAACC TCACGGGAAA GGCACCCAAC ATCCTCCTCT ATGTGGGCTC CGACTCCCAG 
GAAGCCCTCG GCCGGTTCCA CGAGGTCCGG TCTGTGCTGG CCGACTGTGT GGACATTGAC 
AGTTATATTC TCTACCACCT GCTGGAGGAC AGTGCTCTCA GAGACCCGTG GACGGACAAC 
TGTCTGCTGT TGGTCATTGC TACCAGGGAG TCCATTCCCG AAGACCTGTA CCAGAAGTTC 
ATGGCCTATC TTTCTCAGGG AGGGAAGGTG TTGGGCCTGT CTTCATCCTT CACCTTTGGT 
GGCTTTCAGG TGACAAGCAA GGGTGCACTG CACAAGACAG TCCAGAACTT GGTTTTCTCC 
AAGGCTGACC AGAGCGAGGT GAAGCTCAGC GTCTTGAGCA GTGGCTGCAG GTACCAGGAA 
GGCCCCGTCC GGCTCAGCCC CGGCAGGCTC CAGGGCCACC TGGAGAATGA GGACAAGGAC 
AGGATGATTG TGCATGTGCC TTTTGGAACT CGCGGGGGAG AAGCTGTTCT TTGCCAGGTG 
CACTTAGAAC TACCTCCCAG CTCCAACATA GTGCAAACTC CAGAAGATTT TAACTTGCTC 
AAGTCAAGCA ATTTTAGAAG ATACGAAGTC CTTAGAGAGA TTCTGACAAC CCTTGGCCTC 
AGCTGTGACA TGAAACAAGT TCCTGCCTTA ACTCCTCTTT ACTTGCTGTC AGCTGCGGAG 
GAAATCAGGG ATCCTCTTAT GCAGTGGCTT GGGAAACATG TGGACTCCGA GGGAGAAATA 
AAATCCGGCC AGCTCTCTCT TAGATTTGTT TCATCCTACG TGTCTGAAGT AGAAATAACC 
CCATCTTGTA TACCTGTGGT GACCAACATG GAGGCCTTCT CATCAGAACA TTTCAACTTA 
GAGATCTATC GCCAAAATCT GCAGACCAAG CAGTTGGGGA AAGTAATTTT GTTTGCCGAA 
GTGACCCCCA CAACGATGCG TCTCCTGGAT GGGGCCGGAT GTACCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Holocarboxylase Synthetase: A Moonlighting Transcriptional Coregulator of Gene Expression and a Cytosolic Regulator of Biotin Utilization.
Annual review of nutrition37207-223(2017 Aug)
Le?n-Del-R?o A,Valadez-Graham V,Gravel RA

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Holocarboxylase synthetase interacts physically with nuclear receptor co-repressor, histone deacetylase 1 and a novel splicing variant of histone deacetylase 1 to repress repeats.
The Biochemical journal461(3)477-86(2014 Aug)
Liu D,Zempleni J

Holocarboxylase synthetase acts as a biotin-independent transcriptional repressor interacting with HDAC1, HDAC2 and HDAC7.
Molecular genetics and metabolism111(3)321-330(2014 Mar)
Trujillo-Gonzalez I,Cervantes-Roldan R,Gonzalez-Noriega A,Michalak C,Reyes-Carmona S,Barrios-Garcia T,Meneses-Morales I,Leon-Del-Rio A

Holocarboxylase synthetase catalyzes biotinylation of heat shock protein 72, thereby inducing RANTES expression in HEK-293 cells.
American journal of physiology. Cell physiology305(12)C1240-5(2013 Dec)
Xue J,Zhou J,Zempleni J

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HLCS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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