SMURF1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMURF1
Entrez Gene ID	472670
Full Name	SMAD specific E3 ubiquitin protein ligase 1
General protein information	Preferred Names SMAD specific E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase SMURF1 Smad ubiquitination regulatory factor 1
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003318608.3	XP_003318656.1	E3 ubiquitin-protein ligase SMURF1 isoform X2
XM_003318608.2	XP_003318656.1	E3 ubiquitin-protein ligase SMURF1 isoform X2
XM_528043.6	XP_528043.3	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_528043.5	XP_528043.3	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_009453689.2	XP_009451964.1	E3 ubiquitin-protein ligase SMURF1 isoform X3
XM_009453689.3	XP_009451964.1	E3 ubiquitin-protein ligase SMURF1 isoform X3

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SMURF1	XP_414794.3
Homo sapiens (human)	SMURF1	NP_065162.1
	SMURF1	XP_002803251.1
Canis lupus familiaris (dog)	SMURF1	XP_005621234.1
Xenopus tropicalis (tropical clawed frog)	LOC100491582	XP_004917897.1
Pan troglodytes (chimpanzee)	SMURF1	XP_528043.3
Bos taurus (cattle)	SMURF1	XP_002698191.3
Mus musculus (house mouse)	Smurf1	NP_001033716.1
Rattus norvegicus (Norway rat)	Smurf1	NP_001103068.1
Danio rerio (zebrafish)	smurf1	NP_001001943.1

Gene Ontology

The following SMURF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMURF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc57453	XM_528043.6 Latest version!	Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPc57454	XM_003318608.3 Latest version!	Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPc57455	XM_009453689.3 Latest version!	Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OPc57454	XM_003318608.2	Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPc57453	XM_528043.5	Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPc57455	XM_009453689.2	Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMURF1 ( XM_528043.6 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_528043.3 Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc57453
Clone ID Related Accession (Same CDS sequence)	XM_528043.6 , XM_528043.5
Accession Version	XM_528043.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_528043.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CCGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGGAAAACAG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCGGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ACTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ACACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG 
AATTGTGATG AACTTGGACC ACTGCCACCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGC 
GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG 
AAGCTGAAAG TCCTCAGACA TGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC 
ATCGAAGTGT CCAGAGAAGA AATCTTCGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA 
CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC 
GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC 
GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA 
ATCAACCCTG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG 
TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTATAAGCA GCTGCTGGGG 
AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG 
TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC 
GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCGGTCACA 
GAGGAGAACA AGAAAGAATA CGTTCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG 
AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GTCTGGATAA AATAGACTTG 
AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG 
TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT 
GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC 
GCGGCAGGGC CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG 
AAGGCCCATA CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC 
TACGAGAAGC TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_528043.3
CDS	351..2624
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPSGTIPGGDAAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_528043.6
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_528043.6

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CCGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGGAAAACAG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCGGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ACTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ACACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG 
AATTGTGATG AACTTGGACC ACTGCCACCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGC 
GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG 
AAGCTGAAAG TCCTCAGACA TGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC 
ATCGAAGTGT CCAGAGAAGA AATCTTCGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA 
CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC 
GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC 
GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA 
ATCAACCCTG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG 
TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTATAAGCA GCTGCTGGGG 
AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG 
TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC 
GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCGGTCACA 
GAGGAGAACA AGAAAGAATA CGTTCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG 
AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GTCTGGATAA AATAGACTTG 
AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG 
TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT 
GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC 
GCGGCAGGGC CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG 
AAGGCCCATA CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC 
TACGAGAAGC TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_528043.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_528043.3 Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc57453
Clone ID Related Accession (Same CDS sequence)	XM_528043.6 , XM_528043.5
Accession Version	XM_528043.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_528043.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CCGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGGAAAACAG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCGGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ACTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ACACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG 
AATTGTGATG AACTTGGACC ACTGCCACCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGC 
GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG 
AAGCTGAAAG TCCTCAGACA TGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC 
ATCGAAGTGT CCAGAGAAGA AATCTTCGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA 
CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC 
GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC 
GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA 
ATCAACCCTG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG 
TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTATAAGCA GCTGCTGGGG 
AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG 
TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC 
GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCGGTCACA 
GAGGAGAACA AGAAAGAATA CGTTCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG 
AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GTCTGGATAA AATAGACTTG 
AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG 
TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT 
GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC 
GCGGCAGGGC CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG 
AAGGCCCATA CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC 
TACGAGAAGC TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_528043.3
CDS	351..2624
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPSGTIPGGDAAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_528043.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_528043.5

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CCGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGGAAAACAG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCGGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ACTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ACACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG 
AATTGTGATG AACTTGGACC ACTGCCACCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGC 
GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG 
AAGCTGAAAG TCCTCAGACA TGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC 
ATCGAAGTGT CCAGAGAAGA AATCTTCGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA 
CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC 
GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC 
GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA 
ATCAACCCTG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG 
TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTATAAGCA GCTGCTGGGG 
AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG 
TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC 
GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCGGTCACA 
GAGGAGAACA AGAAAGAATA CGTTCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG 
AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GTCTGGATAA AATAGACTTG 
AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG 
TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT 
GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC 
GCGGCAGGGC CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG 
AAGGCCCATA CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC 
TACGAGAAGC TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_003318608.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_003318656.1 Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc57454
Clone ID Related Accession (Same CDS sequence)	XM_003318608.3 , XM_003318608.2
Accession Version	XM_003318608.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_003318608.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CCGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGGAAAACAG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCGGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ACTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ACACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAATTGTGA TGAACTTGGA 
CCACTGCCAC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GCGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CATGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTCG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC TGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTATAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCGGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTTCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGTCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGTTCTACAG GCGCGGCAGG GCCCCGGCTG 
TTCACCATCC ACCTGATAGA CGCGAACACA GACAACCTTC CGAAGGCCCA TACCTGCTTT 
AACCGGATCG ACATTCCACC ATATGAGTCC TATGAGAAGC TCTACGAGAA GCTGCTGACA 
GCCGTGGAGG AGACCTGCGG GTTTGCTGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003318656.1
CDS	351..2546
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_003318608.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003318608.3

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CCGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGGAAAACAG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCGGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ACTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ACACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAATTGTGA TGAACTTGGA 
CCACTGCCAC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GCGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CATGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTCG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC TGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTATAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCGGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTTCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGTCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGTTCTACAG GCGCGGCAGG GCCCCGGCTG 
TTCACCATCC ACCTGATAGA CGCGAACACA GACAACCTTC CGAAGGCCCA TACCTGCTTT 
AACCGGATCG ACATTCCACC ATATGAGTCC TATGAGAAGC TCTACGAGAA GCTGCTGACA 
GCCGTGGAGG AGACCTGCGG GTTTGCTGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_003318608.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_003318656.1 Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc57454
Clone ID Related Accession (Same CDS sequence)	XM_003318608.3 , XM_003318608.2
Accession Version	XM_003318608.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_003318608.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CCGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGGAAAACAG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCGGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ACTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ACACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAATTGTGA TGAACTTGGA 
CCACTGCCAC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GCGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CATGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTCG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC TGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTATAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCGGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTTCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGTCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGTTCTACAG GCGCGGCAGG GCCCCGGCTG 
TTCACCATCC ACCTGATAGA CGCGAACACA GACAACCTTC CGAAGGCCCA TACCTGCTTT 
AACCGGATCG ACATTCCACC ATATGAGTCC TATGAGAAGC TCTACGAGAA GCTGCTGACA 
GCCGTGGAGG AGACCTGCGG GTTTGCTGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003318656.1
CDS	351..2546
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_003318608.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003318608.2

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CCGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGGAAAACAG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCGGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ACTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ACACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAATTGTGA TGAACTTGGA 
CCACTGCCAC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GCGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CATGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTCG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC TGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTATAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCGGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTTCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGTCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGTTCTACAG GCGCGGCAGG GCCCCGGCTG 
TTCACCATCC ACCTGATAGA CGCGAACACA GACAACCTTC CGAAGGCCCA TACCTGCTTT 
AACCGGATCG ACATTCCACC ATATGAGTCC TATGAGAAGC TCTACGAGAA GCTGCTGACA 
GCCGTGGAGG AGACCTGCGG GTTTGCTGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_009453689.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451964.1 Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc57455
Clone ID Related Accession (Same CDS sequence)	XM_009453689.2 , XM_009453689.3
Accession Version	XM_009453689.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009453689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CCGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGGAAAACAG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCGGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ACTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ACACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAATTGTGA TGAACTTGGA 
CCACTGCCAC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GCGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CATGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTCG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC TGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTATAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCGGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTTCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGTCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATC 
CACCTGATAG ACGCGAACAC AGACAACCTT CCGAAGGCCC ATACCTGCTT TAACCGGATC 
GACATTCCAC CATATGAGTC CTATGAGAAG CTCTACGAGA AGCTGCTGAC AGCCGTGGAG 
GAGACCTGCG GGTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451964.1
CDS	351..2537
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_009453689.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009453689.2

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CCGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGGAAAACAG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCGGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ACTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ACACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAATTGTGA TGAACTTGGA 
CCACTGCCAC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GCGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CATGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTCG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC TGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTATAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCGGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTTCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGTCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATC 
CACCTGATAG ACGCGAACAC AGACAACCTT CCGAAGGCCC ATACCTGCTT TAACCGGATC 
GACATTCCAC CATATGAGTC CTATGAGAAG CTCTACGAGA AGCTGCTGAC AGCCGTGGAG 
GAGACCTGCG GGTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_009453689.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451964.1 Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

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CloneID	OPc57455
Clone ID Related Accession (Same CDS sequence)	XM_009453689.2 , XM_009453689.3
Accession Version	XM_009453689.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009453689.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CCGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGGAAAACAG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCGGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ACTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ACACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAATTGTGA TGAACTTGGA 
CCACTGCCAC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GCGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CATGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTCG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC TGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTATAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCGGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTTCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGTCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATC 
CACCTGATAG ACGCGAACAC AGACAACCTT CCGAAGGCCC ATACCTGCTT TAACCGGATC 
GACATTCCAC CATATGAGTC CTATGAGAAG CTCTACGAGA AGCTGCTGAC AGCCGTGGAG 
GAGACCTGCG GGTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451964.1
CDS	351..2537
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_009453689.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009453689.3

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CCGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGGAAAACAG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCGGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ACTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ACACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAATTGTGA TGAACTTGGA 
CCACTGCCAC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GCGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CATGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTCG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC TGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTATAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCGGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTTCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGTCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATC 
CACCTGATAG ACGCGAACAC AGACAACCTT CCGAAGGCCC ATACCTGCTT TAACCGGATC 
GACATTCCAC CATATGAGTC CTATGAGAAG CTCTACGAGA AGCTGCTGAC AGCCGTGGAG 
GAGACCTGCG GGTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SMURF1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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