SMURF1 cDNA ORF clone, Homo sapiens(human)

The following SMURF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMURF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu55935 NM_020429.3
Latest version!
Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu06128 NM_001199847.2
Latest version!
Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu55910 NM_181349.3
Latest version!
Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu97406 XM_017012457.1
Latest version!
Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu55935 NM_020429.2 Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu55910 NM_181349.2 Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu06128 NM_001199847.1 Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu55935
    Clone ID Related Accession (Same CDS sequence) NM_020429.3 , NM_020429.2
    Accession Version NM_020429.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-19
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase SMURF1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727777.1 and BC152468.1. On Jun 2, 2019 this sequence version replaced NM_020429.2. Transcript Variant: This variant (1) encodes the longer isoform (1) of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC152468.1, AB046845.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT
    TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG
    AACTGTGATG AACTTGGACC ACTGCCGCCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG
    AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC
    CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGT
    GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG
    AAGCTGAAAG TCCTCAGACA CGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC
    ATCGAAGTGT CCAGAGAAGA AATCTTTGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA
    CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC
    GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC
    GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA
    ATCAACCCCG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG
    TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTACAAGCA GCTGCTGGGG
    AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG
    TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC
    GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCAGTCACA
    GAGGAGAATA AGAAAGAATA CGTCCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC
    GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG
    AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG
    AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG
    TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT
    GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC
    GCGGCAGGGC CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG
    AAGGCCCATA CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC
    TACGAGAAGC TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065162.1
    CDS329..2602
    Translation

    Target ORF information:

    RefSeq Version NM_020429.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020429.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT
    TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG
    AACTGTGATG AACTTGGACC ACTGCCGCCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG
    AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC
    CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGT
    GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG
    AAGCTGAAAG TCCTCAGACA CGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC
    ATCGAAGTGT CCAGAGAAGA AATCTTTGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA
    CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC
    GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC
    GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA
    ATCAACCCCG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG
    TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTACAAGCA GCTGCTGGGG
    AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG
    TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC
    GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCAGTCACA
    GAGGAGAATA AGAAAGAATA CGTCCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC
    GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG
    AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG
    AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG
    TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT
    GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC
    GCGGCAGGGC CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG
    AAGGCCCATA CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC
    TACGAGAAGC TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu55935
    Clone ID Related Accession (Same CDS sequence) NM_020429.3 , NM_020429.2
    Accession Version NM_020429.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase SMURF1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727777.1, BC152468.1 and BM981719.1. On Dec 23, 2010 this sequence version replaced NM_020429.1. Transcript Variant: This variant (1) encodes the longer isoform (1) of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC152468.1, AB046845.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT
    TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG
    AACTGTGATG AACTTGGACC ACTGCCGCCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG
    AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC
    CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGT
    GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG
    AAGCTGAAAG TCCTCAGACA CGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC
    ATCGAAGTGT CCAGAGAAGA AATCTTTGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA
    CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC
    GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC
    GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA
    ATCAACCCCG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG
    TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTACAAGCA GCTGCTGGGG
    AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG
    TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC
    GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCAGTCACA
    GAGGAGAATA AGAAAGAATA CGTCCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC
    GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG
    AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG
    AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG
    TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT
    GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC
    GCGGCAGGGC CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG
    AAGGCCCATA CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC
    TACGAGAAGC TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065162.1
    CDS341..2614
    Translation

    Target ORF information:

    RefSeq Version NM_020429.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020429.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT
    TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG
    AACTGTGATG AACTTGGACC ACTGCCGCCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG
    AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC
    CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGT
    GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG
    AAGCTGAAAG TCCTCAGACA CGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC
    ATCGAAGTGT CCAGAGAAGA AATCTTTGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA
    CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC
    GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC
    GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA
    ATCAACCCCG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG
    TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTACAAGCA GCTGCTGGGG
    AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG
    TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC
    GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCAGTCACA
    GAGGAGAATA AGAAAGAATA CGTCCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC
    GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG
    AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG
    AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG
    TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT
    GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC
    GCGGCAGGGC CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG
    AAGGCCCATA CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC
    TACGAGAAGC TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06128
    Clone ID Related Accession (Same CDS sequence) NM_001199847.1 , NM_001199847.2
    Accession Version NM_001199847.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-02
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase SMURF1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727777.1, BC144414.1, BC136804.1, BC152468.1 and BM981719.1. Transcript Variant: This variant (3) lacks an in-frame exon in the coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. Isoform 3 is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.45337.1, BC136804.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA
    CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT
    CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG
    TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC
    GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA
    CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA
    GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA
    CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG
    TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT
    ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG
    TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC
    AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA
    GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC
    ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG
    CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA
    TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT
    CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG
    GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC
    ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG
    GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA
    GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATC
    CACCTGATAG ACGCGAACAC AGACAACCTT CCGAAGGCCC ATACCTGCTT TAACCGGATC
    GACATTCCAC CATATGAGTC CTATGAGAAG CTCTACGAGA AGCTGCTGAC AGCCGTGGAG
    GAGACCTGCG GGTTTGCTGT GGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186776.1
    CDS341..2527
    Translation

    Target ORF information:

    RefSeq Version NM_001199847.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199847.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA
    CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT
    CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG
    TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC
    GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA
    CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA
    GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA
    CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG
    TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT
    ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG
    TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC
    AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA
    GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC
    ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG
    CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA
    TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT
    CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG
    GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC
    ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG
    GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA
    GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATC
    CACCTGATAG ACGCGAACAC AGACAACCTT CCGAAGGCCC ATACCTGCTT TAACCGGATC
    GACATTCCAC CATATGAGTC CTATGAGAAG CTCTACGAGA AGCTGCTGAC AGCCGTGGAG
    GAGACCTGCG GGTTTGCTGT GGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06128
    Clone ID Related Accession (Same CDS sequence) NM_001199847.1 , NM_001199847.2
    Accession Version NM_001199847.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase SMURF1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727777.1, BC144414.1, BC136804.1 and BC152468.1. On Jun 2, 2019 this sequence version replaced NM_001199847.1. Transcript Variant: This variant (3) lacks an in-frame exon in the coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. Isoform 3 is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.45337.1, BC136804.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA
    CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT
    CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG
    TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC
    GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA
    CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA
    GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA
    CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG
    TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT
    ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG
    TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC
    AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA
    GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC
    ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG
    CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA
    TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT
    CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG
    GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC
    ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG
    GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA
    GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATC
    CACCTGATAG ACGCGAACAC AGACAACCTT CCGAAGGCCC ATACCTGCTT TAACCGGATC
    GACATTCCAC CATATGAGTC CTATGAGAAG CTCTACGAGA AGCTGCTGAC AGCCGTGGAG
    GAGACCTGCG GGTTTGCTGT GGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186776.1
    CDS329..2515
    Translation

    Target ORF information:

    RefSeq Version NM_001199847.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199847.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA
    CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT
    CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG
    TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC
    GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA
    CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA
    GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA
    CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG
    TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT
    ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG
    TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC
    AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA
    GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC
    ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG
    CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA
    TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT
    CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG
    GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC
    ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG
    GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA
    GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATC
    CACCTGATAG ACGCGAACAC AGACAACCTT CCGAAGGCCC ATACCTGCTT TAACCGGATC
    GACATTCCAC CATATGAGTC CTATGAGAAG CTCTACGAGA AGCTGCTGAC AGCCGTGGAG
    GAGACCTGCG GGTTTGCTGT GGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu55910
    Clone ID Related Accession (Same CDS sequence) NM_181349.2 , NM_181349.3
    Accession Version NM_181349.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-02
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase SMURF1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727777.1, AF199364.2, BC152468.1 and BM981719.1. On Dec 23, 2010 this sequence version replaced NM_181349.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. Isoform 2 is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF199364.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA
    CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT
    CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG
    TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC
    GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA
    CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA
    GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA
    CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG
    TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT
    ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG
    TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC
    AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA
    GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC
    ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG
    CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA
    TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT
    CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG
    GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC
    ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG
    GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA
    GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGTTCTACAG GCGCGGCAGG GCCCCGGCTG
    TTCACCATCC ACCTGATAGA CGCGAACACA GACAACCTTC CGAAGGCCCA TACCTGCTTT
    AACCGGATCG ACATTCCACC ATATGAGTCC TATGAGAAGC TCTACGAGAA GCTGCTGACA
    GCCGTGGAGG AGACCTGCGG GTTTGCTGTG GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_851994.1
    CDS341..2536
    Translation

    Target ORF information:

    RefSeq Version NM_181349.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_181349.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA
    CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT
    CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG
    TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC
    GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA
    CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA
    GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA
    CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG
    TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT
    ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG
    TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC
    AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA
    GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC
    ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG
    CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA
    TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT
    CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG
    GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC
    ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG
    GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA
    GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGTTCTACAG GCGCGGCAGG GCCCCGGCTG
    TTCACCATCC ACCTGATAGA CGCGAACACA GACAACCTTC CGAAGGCCCA TACCTGCTTT
    AACCGGATCG ACATTCCACC ATATGAGTCC TATGAGAAGC TCTACGAGAA GCTGCTGACA
    GCCGTGGAGG AGACCTGCGG GTTTGCTGTG GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu55910
    Clone ID Related Accession (Same CDS sequence) NM_181349.2 , NM_181349.3
    Accession Version NM_181349.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase SMURF1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727777.1, AF199364.2 and BC152468.1. On Jun 2, 2019 this sequence version replaced NM_181349.2. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. Isoform 2 is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF199364.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA
    CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT
    CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG
    TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC
    GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA
    CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA
    GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA
    CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG
    TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT
    ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG
    TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC
    AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA
    GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC
    ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG
    CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA
    TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT
    CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG
    GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC
    ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG
    GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA
    GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGTTCTACAG GCGCGGCAGG GCCCCGGCTG
    TTCACCATCC ACCTGATAGA CGCGAACACA GACAACCTTC CGAAGGCCCA TACCTGCTTT
    AACCGGATCG ACATTCCACC ATATGAGTCC TATGAGAAGC TCTACGAGAA GCTGCTGACA
    GCCGTGGAGG AGACCTGCGG GTTTGCTGTG GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_851994.1
    CDS329..2524
    Translation

    Target ORF information:

    RefSeq Version NM_181349.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_181349.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA
    CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT
    CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG
    TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC
    GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA
    CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA
    GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA
    CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG
    TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT
    ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG
    TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC
    AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA
    GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC
    ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG
    CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA
    TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT
    CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG
    GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC
    ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG
    GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA
    GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGTTCTACAG GCGCGGCAGG GCCCCGGCTG
    TTCACCATCC ACCTGATAGA CGCGAACACA GACAACCTTC CGAAGGCCCA TACCTGCTTT
    AACCGGATCG ACATTCCACC ATATGAGTCC TATGAGAAGC TCTACGAGAA GCTGCTGACA
    GCCGTGGAGG AGACCTGCGG GTTTGCTGTG GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu97406
    Clone ID Related Accession (Same CDS sequence) XM_017012457.1
    Accession Version XM_017012457.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT
    TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG
    AACTGTGATG AACTTGGACC ACTGCCGCCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG
    AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC
    CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGT
    GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG
    AAGCTGAAAG TCCTCAGACA CGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC
    ATCGAAGTGT CCAGAGAAGA AATCTTTGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA
    CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC
    GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC
    GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA
    ATCAACCCCG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG
    TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTACAAGCA GCTGCTGGGG
    AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG
    TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC
    GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCAGTCACA
    GAGGAGAATA AGAAAGAATA CGTCCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC
    GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG
    AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG
    AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG
    TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT
    GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG CGCGGCAGGG
    CCCCGGCTGT TCACCATCCA CCTGATAGAC GCGAACACAG ACAACCTTCC GAAGGCCCAT
    ACCTGCTTTA ACCGGATCGA CATTCCACCA TATGAGTCCT ATGAGAAGCT CTACGAGAAG
    CTGCTGACAG CCGTGGAGGA GACCTGCGGG TTTGCTGTGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016867946.1
    CDS339..2603
    Translation

    Target ORF information:

    RefSeq Version XM_017012457.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017012457.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
    TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT
    GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA
    AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG
    TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG
    CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA
    CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA
    GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA
    ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC
    CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC
    CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA
    CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC
    GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC
    ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT
    TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG
    AACTGTGATG AACTTGGACC ACTGCCGCCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG
    AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC
    CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGT
    GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG
    AAGCTGAAAG TCCTCAGACA CGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC
    ATCGAAGTGT CCAGAGAAGA AATCTTTGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA
    CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC
    GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC
    GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA
    ATCAACCCCG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG
    TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTACAAGCA GCTGCTGGGG
    AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG
    TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC
    GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCAGTCACA
    GAGGAGAATA AGAAAGAATA CGTCCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC
    GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG
    AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG
    AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG
    TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT
    GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG CGCGGCAGGG
    CCCCGGCTGT TCACCATCCA CCTGATAGAC GCGAACACAG ACAACCTTCC GAAGGCCCAT
    ACCTGCTTTA ACCGGATCGA CATTCCACCA TATGAGTCCT ATGAGAAGCT CTACGAGAAG
    CTGCTGACAG CCGTGGAGGA GACCTGCGGG TTTGCTGTGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.