Smurf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smurf1
Entrez Gene ID	690516
Full Name	SMAD specific E3 ubiquitin protein ligase 1
Synonyms	RGD1309707
General protein information	Preferred Names SMAD specific E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase SMURF1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12p11

mRNA and Protein(s)

mRNA	Protein	Name
XM_006248882.1	XP_006248944.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_017598462.1	XP_017453951.1	E3 ubiquitin-protein ligase SMURF1 isoform X2
XM_008769002.1	XP_008767224.1	E3 ubiquitin-protein ligase SMURF1 isoform X3
NM_001109598.1	NP_001103068.1	E3 ubiquitin-protein ligase SMURF1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SMURF1	XP_414794.3
Homo sapiens (human)	SMURF1	NP_065162.1
	SMURF1	XP_002803251.1
Canis lupus familiaris (dog)	SMURF1	XP_005621234.1
Xenopus tropicalis (tropical clawed frog)	LOC100491582	XP_004917897.1
Pan troglodytes (chimpanzee)	SMURF1	XP_528043.3
Bos taurus (cattle)	SMURF1	XP_002698191.3
Mus musculus (house mouse)	Smurf1	NP_001033716.1
Rattus norvegicus (Norway rat)	Smurf1	NP_001103068.1
Danio rerio (zebrafish)	smurf1	NP_001001943.1

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-424(322) as a new marker of disease progression in pulmonary arterial hypertension and its role in right ventricular hypertrophy by targeting SMURF1.
Baptista R, Marques C, Catarino S, Enguita FJ, Costa MC, Matafome P, Zuzarte M, Castro G, Reis A, Monteiro P, P?go M, Pereira P, Gir?o H
Cardiovascular research114(1)53-64(2018 Jan)

The Role of Smurf1 in Neuronal Necroptosis after Lipopolysaccharide-Induced Neuroinflammation.
Shao L, Liu X, Zhu S, Liu C, Gao Y, Xu X
Cellular and molecular neurobiology(2017 Sep)

MiR-503 Promotes Bone Formation in Distraction Osteogenesis through Suppressing Smurf1 Expression.
Sun Y, Xu J, Xu L, Zhang J, Chan K, Pan X, Li G
Scientific reports7(1)409(2017 Mar)

MicroRNA-140-5p and SMURF1 regulate pulmonary arterial hypertension.
Rothman AM, Arnold ND, Pickworth JA, Iremonger J, Ciuclan L, Allen RM, Guth-Gundel S, Southwood M, Morrell NW, Thomas M, Francis SE, Rowlands DJ, Lawrie A
The Journal of clinical investigation126(7)2495-508(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Smurf1 mediates NGF signaling and ubiquitinates RhoA in growth cones, regulating the need for local translation and degradation.
Title: Coupled local translation and degradation regulate growth cone collapse.

Smurf1 might be involved in the pathophysiology of spinal cord after spinal cord injury.
Title: Up-regulation of Smurf1 after spinal cord injury in adult rats.

Smurf1 stability is suppressed by SCF(FBXL15)-mediated ubiquitination.
Title: SCFFBXL?? regulates BMP signalling by directing the degradation of HECT-type ubiquitin ligase Smurf1.

The following Smurf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smurf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12922	NM_001109598.1 Latest version!	Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ORa34616	XM_006248882.1 Latest version!	Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa50498	XM_008769002.1 Latest version!	Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa57898	XM_017598462.1 Latest version!	Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smurf1 ( NM_001109598.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001103068.1 Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), mRNA.

Price & Availability↑

CloneID	ORa12922
Clone ID Related Accession (Same CDS sequence)	NM_001109598.1
Accession Version	NM_001109598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase SMURF1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474012.1. On Oct 4, 2007 this sequence version replaced XM_001074660.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTACGTT GGGAAAACCG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAT ACCGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GTGGTGGAGG GTCGGTGGTG GACTGCAGGG GACTGCTGGA GAACGAAGGA 
ACGGTATATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCGTATACAG ATGGCACCGG TGCAGCGGCA GGAGGTGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGATCC 
CTACAGACAC CGCAGAATCG ACCACATGGC CACCAGTCAC CGGAGCTGCC TGAAGGCTAC 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGC 
ACATGGCACG ACCCCAGGAT CCCCAGAGAC CTTAACAGTG TGAACTGCGA TGAACTTGGG 
CCACTGCCTC CAGGCTGGGA AGTCAGAAGC ACAGTGTCGG GAAGAATCTA TTTTGTAGAT 
CACAATAATA GGACAACCCA GTTTACAGAC CCACGGCTTC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CAGTTGCCCA GTGAGGGCTC CGTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATATGAGAGA GATTTAGTCC AGAAGCTTAA AGTTCTCAGA 
CATGAGCTCT CTCTTCAGCA GCCCCAAGCT GGTCATTGTC GCATAGAAGT TTCCAGAGAA 
GAAATATTTG AGGAGTCATA TCGCCAGATC ATGAAGATGC GACCAAAAGA CCTGAAGAAG 
CGCCTGATGG TGAAGTTCCG AGGGGAGGAA GGCTTGGACT ATGGTGGGGT GGCTCGGGAG 
TGGCTGTATT TGTTGTGCCA TGAAATGTTG AATCCGTATT ATGGACTCTT CCAGTATTCC 
ACGGACAATA TTTACACACT GCAAATCAAC CCAGATTCTT CTATCAACCC TGACCATCTG 
TCTTACTTCC ACTTTGTGGG TCGCATCATG GGTCTGGCTG TGTTCCATGG ACACTACATA 
AACGGGGGCT TCACAGTTCC TTTCTACAAG CAGCTCCTGG GAAAACCCAT CCAGCTGTCG 
GATCTGGAGT CAGTGGACCC AGAACTTCAC AAGAGCTTGG TGTGGATTCT AGAGAATGAC 
ATCACGCCAG TGCTGGATCA CACCTTCTGC GTGGAGCACA ATGCTTTTGG GCGGATCCTT 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCTGTCA CTGAGGAGAA CAAGAAGGAG 
TATGTGCGGT TGTATGTGAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCCTAGCG 
CTTCAGAAAG GGTTTAACGA ACTCATTCCC CAACACTTAC TGAAGCCCTT TGACCAGAAG 
GAACTAGAGC TGATAATAGG CGGCCTGGAT AAGATAGACC TGAATGACTG GAAGTCCAAC 
ACTCGGCTGA AGCACTGTGT GGCAGACAGC AACATCGTCA GGTGGTTCTG GCAGGCGGTG 
GAGACCTTCG ATGAGGAGAG GAGAGCCAGG CTCCTGCAGT TTGTGACAGG ATCCACACGA 
GTTCCTCTCC AAGGCTTCAA GGCTCTGCAA GGCGCGGCAG GGCCCCGGCT CTTCACCATC 
CACCTGATTG ACGCCAACAC CGACAACCTG CCCAAGGCCC ACACCTGCTT TAATCGGATT 
GACATCCCAC CCTATGAGTC CTATGAGAAG CTCTATGAGA AGCTGCTGAC AGCGGTAGAG 
GAGACCTGTG GCTTTGCCGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103068.1
CDS	23..2209
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	NM_001109598.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109598.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTACGTT GGGAAAACCG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAT ACCGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GTGGTGGAGG GTCGGTGGTG GACTGCAGGG GACTGCTGGA GAACGAAGGA 
ACGGTATATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCGTATACAG ATGGCACCGG TGCAGCGGCA GGAGGTGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGATCC 
CTACAGACAC CGCAGAATCG ACCACATGGC CACCAGTCAC CGGAGCTGCC TGAAGGCTAC 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGC 
ACATGGCACG ACCCCAGGAT CCCCAGAGAC CTTAACAGTG TGAACTGCGA TGAACTTGGG 
CCACTGCCTC CAGGCTGGGA AGTCAGAAGC ACAGTGTCGG GAAGAATCTA TTTTGTAGAT 
CACAATAATA GGACAACCCA GTTTACAGAC CCACGGCTTC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CAGTTGCCCA GTGAGGGCTC CGTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATATGAGAGA GATTTAGTCC AGAAGCTTAA AGTTCTCAGA 
CATGAGCTCT CTCTTCAGCA GCCCCAAGCT GGTCATTGTC GCATAGAAGT TTCCAGAGAA 
GAAATATTTG AGGAGTCATA TCGCCAGATC ATGAAGATGC GACCAAAAGA CCTGAAGAAG 
CGCCTGATGG TGAAGTTCCG AGGGGAGGAA GGCTTGGACT ATGGTGGGGT GGCTCGGGAG 
TGGCTGTATT TGTTGTGCCA TGAAATGTTG AATCCGTATT ATGGACTCTT CCAGTATTCC 
ACGGACAATA TTTACACACT GCAAATCAAC CCAGATTCTT CTATCAACCC TGACCATCTG 
TCTTACTTCC ACTTTGTGGG TCGCATCATG GGTCTGGCTG TGTTCCATGG ACACTACATA 
AACGGGGGCT TCACAGTTCC TTTCTACAAG CAGCTCCTGG GAAAACCCAT CCAGCTGTCG 
GATCTGGAGT CAGTGGACCC AGAACTTCAC AAGAGCTTGG TGTGGATTCT AGAGAATGAC 
ATCACGCCAG TGCTGGATCA CACCTTCTGC GTGGAGCACA ATGCTTTTGG GCGGATCCTT 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCTGTCA CTGAGGAGAA CAAGAAGGAG 
TATGTGCGGT TGTATGTGAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCCTAGCG 
CTTCAGAAAG GGTTTAACGA ACTCATTCCC CAACACTTAC TGAAGCCCTT TGACCAGAAG 
GAACTAGAGC TGATAATAGG CGGCCTGGAT AAGATAGACC TGAATGACTG GAAGTCCAAC 
ACTCGGCTGA AGCACTGTGT GGCAGACAGC AACATCGTCA GGTGGTTCTG GCAGGCGGTG 
GAGACCTTCG ATGAGGAGAG GAGAGCCAGG CTCCTGCAGT TTGTGACAGG ATCCACACGA 
GTTCCTCTCC AAGGCTTCAA GGCTCTGCAA GGCGCGGCAG GGCCCCGGCT CTTCACCATC 
CACCTGATTG ACGCCAACAC CGACAACCTG CCCAAGGCCC ACACCTGCTT TAATCGGATT 
GACATCCCAC CCTATGAGTC CTATGAGAAG CTCTATGAGA AGCTGCTGAC AGCGGTAGAG 
GAGACCTGTG GCTTTGCCGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_006248882.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248944.1 Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa34616
Clone ID Related Accession (Same CDS sequence)	XM_006248882.1
Accession Version	XM_006248882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTACGTT GGGAAAACCG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAT ACCGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GTGGTGGAGG GTCGGTGGTG GACTGCAGGG GACTGCTGGA GAACGAAGGA 
ACGGTATATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCGTATACAG ATGGCACCGG TGCAGCGGCA GGAGGTGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGATCC 
CTACAGACAC CGCAGAATCG ACCACATGGC CACCAGTCAC CGGAGCTGCC TGAAGGCTAC 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGC 
ACATGGCACG ACCCCAGGAT CCCCAGTCCC TTGGGGACCA TTCCGGGGGG AGATGAAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCCGAGC CCAGAGACCT TAACAGTGTG 
AACTGCGATG AACTTGGGCC ACTGCCTCCA GGCTGGGAAG TCAGAAGCAC AGTGTCGGGA 
AGAATCTATT TTGTAGATCA CAATAATAGG ACAACCCAGT TTACAGACCC ACGGCTTCAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCA GTTGCCCAGT 
GAGGGCTCCG TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ATGAGAGAGA TTTAGTCCAG 
AAGCTTAAAG TTCTCAGACA TGAGCTCTCT CTTCAGCAGC CCCAAGCTGG TCATTGTCGC 
ATAGAAGTTT CCAGAGAAGA AATATTTGAG GAGTCATATC GCCAGATCAT GAAGATGCGA 
CCAAAAGACC TGAAGAAGCG CCTGATGGTG AAGTTCCGAG GGGAGGAAGG CTTGGACTAT 
GGTGGGGTGG CTCGGGAGTG GCTGTATTTG TTGTGCCATG AAATGTTGAA TCCGTATTAT 
GGACTCTTCC AGTATTCCAC GGACAATATT TACACACTGC AAATCAACCC AGATTCTTCT 
ATCAACCCTG ACCATCTGTC TTACTTCCAC TTTGTGGGTC GCATCATGGG TCTGGCTGTG 
TTCCATGGAC ACTACATAAA CGGGGGCTTC ACAGTTCCTT TCTACAAGCA GCTCCTGGGA 
AAACCCATCC AGCTGTCGGA TCTGGAGTCA GTGGACCCAG AACTTCACAA GAGCTTGGTG 
TGGATTCTAG AGAATGACAT CACGCCAGTG CTGGATCACA CCTTCTGCGT GGAGCACAAT 
GCTTTTGGGC GGATCCTTCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCTGTCACT 
GAGGAGAACA AGAAGGAGTA TGTGCGGTTG TATGTGAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCCTAGCGCT TCAGAAAGGG TTTAACGAAC TCATTCCCCA ACACTTACTG 
AAGCCCTTTG ACCAGAAGGA ACTAGAGCTG ATAATAGGCG GCCTGGATAA GATAGACCTG 
AATGACTGGA AGTCCAACAC TCGGCTGAAG CACTGTGTGG CAGACAGCAA CATCGTCAGG 
TGGTTCTGGC AGGCGGTGGA GACCTTCGAT GAGGAGAGGA GAGCCAGGCT CCTGCAGTTT 
GTGACAGGAT CCACACGAGT TCCTCTCCAA GGCTTCAAGG CTCTGCAAGG CTCTACAGGC 
GCGGCAGGGC CCCGGCTCTT CACCATCCAC CTGATTGACG CCAACACCGA CAACCTGCCC 
AAGGCCCACA CCTGCTTTAA TCGGATTGAC ATCCCACCCT ATGAGTCCTA TGAGAAGCTC 
TATGAGAAGC TGCTGACAGC GGTAGAGGAG ACCTGTGGCT TTGCCGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248944.1
CDS	19..2292
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPLGTIPGGDEAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_006248882.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248882.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTACGTT GGGAAAACCG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAT ACCGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GTGGTGGAGG GTCGGTGGTG GACTGCAGGG GACTGCTGGA GAACGAAGGA 
ACGGTATATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCGTATACAG ATGGCACCGG TGCAGCGGCA GGAGGTGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGATCC 
CTACAGACAC CGCAGAATCG ACCACATGGC CACCAGTCAC CGGAGCTGCC TGAAGGCTAC 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGC 
ACATGGCACG ACCCCAGGAT CCCCAGTCCC TTGGGGACCA TTCCGGGGGG AGATGAAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCCGAGC CCAGAGACCT TAACAGTGTG 
AACTGCGATG AACTTGGGCC ACTGCCTCCA GGCTGGGAAG TCAGAAGCAC AGTGTCGGGA 
AGAATCTATT TTGTAGATCA CAATAATAGG ACAACCCAGT TTACAGACCC ACGGCTTCAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCA GTTGCCCAGT 
GAGGGCTCCG TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ATGAGAGAGA TTTAGTCCAG 
AAGCTTAAAG TTCTCAGACA TGAGCTCTCT CTTCAGCAGC CCCAAGCTGG TCATTGTCGC 
ATAGAAGTTT CCAGAGAAGA AATATTTGAG GAGTCATATC GCCAGATCAT GAAGATGCGA 
CCAAAAGACC TGAAGAAGCG CCTGATGGTG AAGTTCCGAG GGGAGGAAGG CTTGGACTAT 
GGTGGGGTGG CTCGGGAGTG GCTGTATTTG TTGTGCCATG AAATGTTGAA TCCGTATTAT 
GGACTCTTCC AGTATTCCAC GGACAATATT TACACACTGC AAATCAACCC AGATTCTTCT 
ATCAACCCTG ACCATCTGTC TTACTTCCAC TTTGTGGGTC GCATCATGGG TCTGGCTGTG 
TTCCATGGAC ACTACATAAA CGGGGGCTTC ACAGTTCCTT TCTACAAGCA GCTCCTGGGA 
AAACCCATCC AGCTGTCGGA TCTGGAGTCA GTGGACCCAG AACTTCACAA GAGCTTGGTG 
TGGATTCTAG AGAATGACAT CACGCCAGTG CTGGATCACA CCTTCTGCGT GGAGCACAAT 
GCTTTTGGGC GGATCCTTCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCTGTCACT 
GAGGAGAACA AGAAGGAGTA TGTGCGGTTG TATGTGAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCCTAGCGCT TCAGAAAGGG TTTAACGAAC TCATTCCCCA ACACTTACTG 
AAGCCCTTTG ACCAGAAGGA ACTAGAGCTG ATAATAGGCG GCCTGGATAA GATAGACCTG 
AATGACTGGA AGTCCAACAC TCGGCTGAAG CACTGTGTGG CAGACAGCAA CATCGTCAGG 
TGGTTCTGGC AGGCGGTGGA GACCTTCGAT GAGGAGAGGA GAGCCAGGCT CCTGCAGTTT 
GTGACAGGAT CCACACGAGT TCCTCTCCAA GGCTTCAAGG CTCTGCAAGG CTCTACAGGC 
GCGGCAGGGC CCCGGCTCTT CACCATCCAC CTGATTGACG CCAACACCGA CAACCTGCCC 
AAGGCCCACA CCTGCTTTAA TCGGATTGAC ATCCCACCCT ATGAGTCCTA TGAGAAGCTC 
TATGAGAAGC TGCTGACAGC GGTAGAGGAG ACCTGTGGCT TTGCCGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_008769002.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767224.1 Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa50498
Clone ID Related Accession (Same CDS sequence)	XM_008769002.1
Accession Version	XM_008769002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTACGTT GGGAAAACCG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAT ACCGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GTGGTGGAGG GTCGGTGGTG GACTGCAGGG GACTGCTGGA GAACGAAGGA 
ACGGTATATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCGTATACAG ATGGCACCGG TGCAGCGGCA GGAGGTGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGATCC 
CTACAGACAC CGCAGAATCG ACCACATGGC CACCAGTCAC CGGAGCTGCC TGAAGGCTAC 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGC 
ACATGGCACG ACCCCAGGAT CCCCAGAGAC CTTAACAGTG TGAACTGCGA TGAACTTGGG 
CCACTGCCTC CAGGCTGGGA AGTCAGAAGC ACAGTGTCGG GAAGAATCTA TTTTGTAGAT 
CACAATAATA GGACAACCCA GTTTACAGAC CCACGGCTTC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CAGTTGCCCA GTGAGGGCTC CGTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATATGAGAGA GATTTAGTCC AGAAGCTTAA AGTTCTCAGA 
CATGAGCTCT CTCTTCAGCA GCCCCAAGCT GGTCATTGTC GCATAGAAGT TTCCAGAGAA 
GAAATATTTG AGGAGTCATA TCGCCAGATC ATGAAGATGC GACCAAAAGA CCTGAAGAAG 
CGCCTGATGG TGAAGTTCCG AGGGGAGGAA GGCTTGGACT ATGGTGGGGT GGCTCGGGAG 
TGGCTGTATT TGTTGTGCCA TGAAATGTTG AATCCGTATT ATGGACTCTT CCAGTATTCC 
ACGGACAATA TTTACACACT GCAAATCAAC CCAGATTCTT CTATCAACCC TGACCATCTG 
TCTTACTTCC ACTTTGTGGG TCGCATCATG GGTCTGGCTG TGTTCCATGG ACACTACATA 
AACGGGGGCT TCACAGTTCC TTTCTACAAG CAGCTCCTGG GAAAACCCAT CCAGCTGTCG 
GATCTGGAGT CAGTGGACCC AGAACTTCAC AAGAGCTTGG TGTGGATTCT AGAGAATGAC 
ATCACGCCAG TGCTGGATCA CACCTTCTGC GTGGAGCACA ATGCTTTTGG GCGGATCCTT 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCTGTCA CTGAGGAGAA CAAGAAGGAG 
TATGTGCGGT TGTATGTGAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCCTAGCG 
CTTCAGAAAG GGTTTAACGA ACTCATTCCC CAACACTTAC TGAAGCCCTT TGACCAGAAG 
GAACTAGAGC TGATAATAGG CGGCCTGGAT AAGATAGACC TGAATGACTG GAAGTCCAAC 
ACTCGGCTGA AGCACTGTGT GGCAGACAGC AACATCGTCA GGTGGTTCTG GCAGGCGGTG 
GAGACCTTCG ATGAGGAGAG GAGAGCCAGG CTCCTGCAGT TTGTGACAGG ATCCACACGA 
GTTCCTCTCC AAGGCTTCAA GGCTCTGCAA GGCTCTACAG GCGCGGCAGG GCCCCGGCTC 
TTCACCATCC ACCTGATTGA CGCCAACACC GACAACCTGC CCAAGGCCCA CACCTGCTTT 
AATCGGATTG ACATCCCACC CTATGAGTCC TATGAGAAGC TCTATGAGAA GCTGCTGACA 
GCGGTAGAGG AGACCTGTGG CTTTGCCGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767224.1
CDS	19..2214
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_008769002.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769002.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTACGTT GGGAAAACCG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAT ACCGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GTGGTGGAGG GTCGGTGGTG GACTGCAGGG GACTGCTGGA GAACGAAGGA 
ACGGTATATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCGTATACAG ATGGCACCGG TGCAGCGGCA GGAGGTGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGATCC 
CTACAGACAC CGCAGAATCG ACCACATGGC CACCAGTCAC CGGAGCTGCC TGAAGGCTAC 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGC 
ACATGGCACG ACCCCAGGAT CCCCAGAGAC CTTAACAGTG TGAACTGCGA TGAACTTGGG 
CCACTGCCTC CAGGCTGGGA AGTCAGAAGC ACAGTGTCGG GAAGAATCTA TTTTGTAGAT 
CACAATAATA GGACAACCCA GTTTACAGAC CCACGGCTTC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CAGTTGCCCA GTGAGGGCTC CGTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATATGAGAGA GATTTAGTCC AGAAGCTTAA AGTTCTCAGA 
CATGAGCTCT CTCTTCAGCA GCCCCAAGCT GGTCATTGTC GCATAGAAGT TTCCAGAGAA 
GAAATATTTG AGGAGTCATA TCGCCAGATC ATGAAGATGC GACCAAAAGA CCTGAAGAAG 
CGCCTGATGG TGAAGTTCCG AGGGGAGGAA GGCTTGGACT ATGGTGGGGT GGCTCGGGAG 
TGGCTGTATT TGTTGTGCCA TGAAATGTTG AATCCGTATT ATGGACTCTT CCAGTATTCC 
ACGGACAATA TTTACACACT GCAAATCAAC CCAGATTCTT CTATCAACCC TGACCATCTG 
TCTTACTTCC ACTTTGTGGG TCGCATCATG GGTCTGGCTG TGTTCCATGG ACACTACATA 
AACGGGGGCT TCACAGTTCC TTTCTACAAG CAGCTCCTGG GAAAACCCAT CCAGCTGTCG 
GATCTGGAGT CAGTGGACCC AGAACTTCAC AAGAGCTTGG TGTGGATTCT AGAGAATGAC 
ATCACGCCAG TGCTGGATCA CACCTTCTGC GTGGAGCACA ATGCTTTTGG GCGGATCCTT 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCTGTCA CTGAGGAGAA CAAGAAGGAG 
TATGTGCGGT TGTATGTGAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCCTAGCG 
CTTCAGAAAG GGTTTAACGA ACTCATTCCC CAACACTTAC TGAAGCCCTT TGACCAGAAG 
GAACTAGAGC TGATAATAGG CGGCCTGGAT AAGATAGACC TGAATGACTG GAAGTCCAAC 
ACTCGGCTGA AGCACTGTGT GGCAGACAGC AACATCGTCA GGTGGTTCTG GCAGGCGGTG 
GAGACCTTCG ATGAGGAGAG GAGAGCCAGG CTCCTGCAGT TTGTGACAGG ATCCACACGA 
GTTCCTCTCC AAGGCTTCAA GGCTCTGCAA GGCTCTACAG GCGCGGCAGG GCCCCGGCTC 
TTCACCATCC ACCTGATTGA CGCCAACACC GACAACCTGC CCAAGGCCCA CACCTGCTTT 
AATCGGATTG ACATCCCACC CTATGAGTCC TATGAGAAGC TCTATGAGAA GCTGCTGACA 
GCGGTAGAGG AGACCTGTGG CTTTGCCGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_017598462.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453951.1 Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa57898
Clone ID Related Accession (Same CDS sequence)	XM_017598462.1
Accession Version	XM_017598462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTACGTT GGGAAAACCG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAT ACCGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GTGGTGGAGG GTCGGTGGTG GACTGCAGGG GACTGCTGGA GAACGAAGGA 
ACGGTATATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCGTATACAG ATGGCACCGG TGCAGCGGCA GGAGGTGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGATCC 
CTACAGACAC CGCAGAATCG ACCACATGGC CACCAGTCAC CGGAGCTGCC TGAAGGCTAC 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGC 
ACATGGCACG ACCCCAGGAT CCCCAGTCCC TTGGGGACCA TTCCGGGGGG AGATGAAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCCGAGC CCAGAGACCT TAACAGTGTG 
AACTGCGATG AACTTGGGCC ACTGCCTCCA GGCTGGGAAG TCAGAAGCAC AGTGTCGGGA 
AGAATCTATT TTGTAGATCA CAATAATAGG ACAACCCAGT TTACAGACCC ACGGCTTCAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCA GTTGCCCAGT 
GAGGGCTCCG TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ATGAGAGAGA TTTAGTCCAG 
AAGCTTAAAG TTCTCAGACA TGAGCTCTCT CTTCAGCAGC CCCAAGCTGG TCATTGTCGC 
ATAGAAGTTT CCAGAGAAGA AATATTTGAG GAGTCATATC GCCAGATCAT GAAGATGCGA 
CCAAAAGACC TGAAGAAGCG CCTGATGGTG AAGTTCCGAG GGGAGGAAGG CTTGGACTAT 
GGTGGGGTGG CTCGGGAGTG GCTGTATTTG TTGTGCCATG AAATGTTGAA TCCGTATTAT 
GGACTCTTCC AGTATTCCAC GGACAATATT TACACACTGC AAATCAACCC AGATTCTTCT 
ATCAACCCTG ACCATCTGTC TTACTTCCAC TTTGTGGGTC GCATCATGGG TCTGGCTGTG 
TTCCATGGAC ACTACATAAA CGGGGGCTTC ACAGTTCCTT TCTACAAGCA GCTCCTGGGA 
AAACCCATCC AGCTGTCGGA TCTGGAGTCA GTGGACCCAG AACTTCACAA GAGCTTGGTG 
TGGATTCTAG AGAATGACAT CACGCCAGTG CTGGATCACA CCTTCTGCGT GGAGCACAAT 
GCTTTTGGGC GGATCCTTCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCTGTCACT 
GAGGAGAACA AGAAGGAGTA TGTGCGGTTG TATGTGAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCCTAGCGCT TCAGAAAGGG TTTAACGAAC TCATTCCCCA ACACTTACTG 
AAGCCCTTTG ACCAGAAGGA ACTAGAGCTG ATAATAGGCG GCCTGGATAA GATAGACCTG 
AATGACTGGA AGTCCAACAC TCGGCTGAAG CACTGTGTGG CAGACAGCAA CATCGTCAGG 
TGGTTCTGGC AGGCGGTGGA GACCTTCGAT GAGGAGAGGA GAGCCAGGCT CCTGCAGTTT 
GTGACAGGAT CCACACGAGT TCCTCTCCAA GGCTTCAAGG CTCTGCAAGG CGCGGCAGGG 
CCCCGGCTCT TCACCATCCA CCTGATTGAC GCCAACACCG ACAACCTGCC CAAGGCCCAC 
ACCTGCTTTA ATCGGATTGA CATCCCACCC TATGAGTCCT ATGAGAAGCT CTATGAGAAG 
CTGCTGACAG CGGTAGAGGA GACCTGTGGC TTTGCCGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453951.1
CDS	19..2283
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPLGTIPGGDEAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_017598462.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598462.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTACGTT GGGAAAACCG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG CGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAT ACCGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GTGGTGGAGG GTCGGTGGTG GACTGCAGGG GACTGCTGGA GAACGAAGGA 
ACGGTATATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCGTATACAG ATGGCACCGG TGCAGCGGCA GGAGGTGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGATCC 
CTACAGACAC CGCAGAATCG ACCACATGGC CACCAGTCAC CGGAGCTGCC TGAAGGCTAC 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGC 
ACATGGCACG ACCCCAGGAT CCCCAGTCCC TTGGGGACCA TTCCGGGGGG AGATGAAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCCGAGC CCAGAGACCT TAACAGTGTG 
AACTGCGATG AACTTGGGCC ACTGCCTCCA GGCTGGGAAG TCAGAAGCAC AGTGTCGGGA 
AGAATCTATT TTGTAGATCA CAATAATAGG ACAACCCAGT TTACAGACCC ACGGCTTCAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCA GTTGCCCAGT 
GAGGGCTCCG TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ATGAGAGAGA TTTAGTCCAG 
AAGCTTAAAG TTCTCAGACA TGAGCTCTCT CTTCAGCAGC CCCAAGCTGG TCATTGTCGC 
ATAGAAGTTT CCAGAGAAGA AATATTTGAG GAGTCATATC GCCAGATCAT GAAGATGCGA 
CCAAAAGACC TGAAGAAGCG CCTGATGGTG AAGTTCCGAG GGGAGGAAGG CTTGGACTAT 
GGTGGGGTGG CTCGGGAGTG GCTGTATTTG TTGTGCCATG AAATGTTGAA TCCGTATTAT 
GGACTCTTCC AGTATTCCAC GGACAATATT TACACACTGC AAATCAACCC AGATTCTTCT 
ATCAACCCTG ACCATCTGTC TTACTTCCAC TTTGTGGGTC GCATCATGGG TCTGGCTGTG 
TTCCATGGAC ACTACATAAA CGGGGGCTTC ACAGTTCCTT TCTACAAGCA GCTCCTGGGA 
AAACCCATCC AGCTGTCGGA TCTGGAGTCA GTGGACCCAG AACTTCACAA GAGCTTGGTG 
TGGATTCTAG AGAATGACAT CACGCCAGTG CTGGATCACA CCTTCTGCGT GGAGCACAAT 
GCTTTTGGGC GGATCCTTCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCTGTCACT 
GAGGAGAACA AGAAGGAGTA TGTGCGGTTG TATGTGAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCCTAGCGCT TCAGAAAGGG TTTAACGAAC TCATTCCCCA ACACTTACTG 
AAGCCCTTTG ACCAGAAGGA ACTAGAGCTG ATAATAGGCG GCCTGGATAA GATAGACCTG 
AATGACTGGA AGTCCAACAC TCGGCTGAAG CACTGTGTGG CAGACAGCAA CATCGTCAGG 
TGGTTCTGGC AGGCGGTGGA GACCTTCGAT GAGGAGAGGA GAGCCAGGCT CCTGCAGTTT 
GTGACAGGAT CCACACGAGT TCCTCTCCAA GGCTTCAAGG CTCTGCAAGG CGCGGCAGGG 
CCCCGGCTCT TCACCATCCA CCTGATTGAC GCCAACACCG ACAACCTGCC CAAGGCCCAC 
ACCTGCTTTA ATCGGATTGA CATCCCACCC TATGAGTCCT ATGAGAAGCT CTATGAGAAG 
CTGCTGACAG CGGTAGAGGA GACCTGTGGC TTTGCCGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-424(322) as a new marker of disease progression in pulmonary arterial hypertension and its role in right ventricular hypertrophy by targeting SMURF1.
Cardiovascular research114(1)53-64(2018 Jan)
Baptista R,Marques C,Catarino S,Enguita FJ,Costa MC,Matafome P,Zuzarte M,Castro G,Reis A,Monteiro P,P?go M,Pereira P,Gir?o H

The Role of Smurf1 in Neuronal Necroptosis after Lipopolysaccharide-Induced Neuroinflammation.
Cellular and molecular neurobiology(2017 Sep)
Shao L,Liu X,Zhu S,Liu C,Gao Y,Xu X

MiR-503 Promotes Bone Formation in Distraction Osteogenesis through Suppressing Smurf1 Expression.
Scientific reports7(1)409(2017 Mar)
Sun Y,Xu J,Xu L,Zhang J,Chan K,Pan X,Li G

MicroRNA-140-5p and SMURF1 regulate pulmonary arterial hypertension.
The Journal of clinical investigation126(7)2495-508(2016 Jul)
Rothman AM,Arnold ND,Pickworth JA,Iremonger J,Ciuclan L,Allen RM,Guth-Gundel S,Southwood M,Morrell NW,Thomas M,Francis SE,Rowlands DJ,Lawrie A

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Smurf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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