SMURF1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005197000.3	XP_005197057.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_005196999.3	XP_005197056.1	E3 ubiquitin-protein ligase SMURF1 isoform X3
XM_002698145.6	XP_002698191.4	E3 ubiquitin-protein ligase SMURF1 isoform X4
XM_005225272.3	XP_005225329.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_010819648.2	XP_010817950.1	E3 ubiquitin-protein ligase SMURF1 isoform X2
XM_010825852.2	XP_010824154.1	E3 ubiquitin-protein ligase SMURF1 isoform X2
XM_005225271.3	XP_005225328.1	E3 ubiquitin-protein ligase SMURF1 isoform X3
XM_002698145.5	XP_002698191.3	E3 ubiquitin-protein ligase SMURF1 isoform X4
XM_591663.7	XP_591663.5	E3 ubiquitin-protein ligase SMURF1 isoform X4
XM_005225272.4	XP_005225329.2	E3 ubiquitin-protein ligase SMURF1 isoform X2
XM_005225271.4	XP_005225328.2	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_024985447.1	XP_024841215.1	E3 ubiquitin-protein ligase SMURF1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa11955	XM_591663.7 Latest version!	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa11955	XM_002698145.5 Latest version!	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa11956	XM_005196999.3 Latest version!	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa11956	XM_005225271.3 Latest version!	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa11957	XM_005197000.3 Latest version!	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa11957	XM_005225272.3 Latest version!	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa48766	XM_010825852.2 Latest version!	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa48766	XM_010819648.2 Latest version!	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa230703	XM_005225271.4 Latest version!	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa230704	XM_005225272.4 Latest version!	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa230705	XM_024985447.1 Latest version!	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa230706	XM_002698145.6 Latest version!	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

SMURF1 ( XM_002698145.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002698191.3 Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa11955
Clone ID Related Accession (Same CDS sequence)	XM_002698145.5 , XM_591663.7
Accession Version	XM_002698145.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002698145.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCTGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA ACTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGTCCC TGGGGGACCA CTCCTGGGGG AGATGGAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGCGTG 
AACTGCGATG AACTTGGACC ACTGCCGCCC GGTTGGGAAG TCCGCAGCAC GGTTTCCGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC GCGGTTACAC 
CACATCATGA CTCACCAGTG CCAACTGAAG GAGCCCAGCC AGCCACCACC GCCACCCAGC 
GAGGGCTCCG TGGAGGACGA GGAGCTGCCC GCCCAGAGGT ACGAGAGAGA TCTGGTCCAG 
AAACTGAAGG TCCTCAGACA CGAGCTCTCC TTGCAGCAGC CCCAGGCTGG CCACTGCCGC 
ATCGAGGTGT CCAGAGAGGA GATATTTGAG GAGTCTTATC GCCAGATAAT GAAGATGCGA 
CCAAAAGACT TGAAAAAGCG ACTCATGGTG AAATTCCGAG GAGAAGAAGG CTTGGACTAT 
GGAGGGGTGG CGAGGGAGTG GCTTTATTTA CTGTGCCATG AAATGCTGAA CCCGTACTAT 
GGACTCTTCC AGTACTCCAC GGACAACATT TACATGTTGC AAATCAACCC AGACTCTTCA 
ATCAACCCTG ACCACTTGTC TTACTTCCAT TTCGTGGGTC GGATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATCAA TGGGGGTTTC ACGGTGCCTT TCTACAAGCA GCTACTGGGG 
AAGCCCATCC AGCTTTCTGA TTTGGAATCG GTGGACCCTG AACTCCATAA GAGCCTAGTG 
TGGATTCTAG AGAATGACAT CACCCCCGTG CTCGATCACA CGTTCTGCGT GGAGCACAAT 
GCTTTCGGGC GGATTCTTCA GCACGAACTG AAACCCAACG GCAGGAACGT CCCCGTCACA 
GAGGAGAATA AGAAGGAATA TGTCCGGTTG TATGTAAATT GGAGGTTCAT GAGGGGAATC 
GAGGCCCAGT TCCTGGCTCT TCAGAAGGGG TTTAACGAGC TCATCCCTCA ACACTTATTG 
AAGCCTTTTG ACCAGAAGGA GCTGGAGCTG ATCATTGGCG GCCTAGACAA GATCGACCTG 
GACGACTGGA AGTCCAACAC GCGGCTGAAG CACTGTGGGG CCGACAGCAA CGTGGTCCGG 
TGGTTCTGGC AGGCGGTAGA GACCTTCGAT GAGGAGCGGC GGGCCCGGCT GCTGCAGTTC 
GTGACAGGCT CCACGCGGGT CCCCCTCCAG GGCTTCAAGG CGCTGCAAGG CTCTACAGGC 
GCCGCAGGGC CCCGGCTCTT CACCATCCAC CTGATCGACG CCAACACGGA CAACCTGCCC 
AAGGCCCACA CCTGCTTTAA CCGGATCGAC ATCCCGCCCT ACGAGTCGTA CGAGAAGCTC 
TATGAGAAGC TGCTGACTGC GGTGGAGGAG ACCTGTGGCT TTGCCGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002698191.3
CDS	77..2350
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLESEGTVYEDSGPGRPLSCFMEEPAPYTDGPGAAAGGGNCRFVESPSQDQRLQAQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPWGTTPGGDGAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMTHQCQLKEPSQPPPPPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_002698145.5
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002698145.5

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCTGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA ACTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGTCCC TGGGGGACCA CTCCTGGGGG AGATGGAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGCGTG 
AACTGCGATG AACTTGGACC ACTGCCGCCC GGTTGGGAAG TCCGCAGCAC GGTTTCCGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC GCGGTTACAC 
CACATCATGA CTCACCAGTG CCAACTGAAG GAGCCCAGCC AGCCACCACC GCCACCCAGC 
GAGGGCTCCG TGGAGGACGA GGAGCTGCCC GCCCAGAGGT ACGAGAGAGA TCTGGTCCAG 
AAACTGAAGG TCCTCAGACA CGAGCTCTCC TTGCAGCAGC CCCAGGCTGG CCACTGCCGC 
ATCGAGGTGT CCAGAGAGGA GATATTTGAG GAGTCTTATC GCCAGATAAT GAAGATGCGA 
CCAAAAGACT TGAAAAAGCG ACTCATGGTG AAATTCCGAG GAGAAGAAGG CTTGGACTAT 
GGAGGGGTGG CGAGGGAGTG GCTTTATTTA CTGTGCCATG AAATGCTGAA CCCGTACTAT 
GGACTCTTCC AGTACTCCAC GGACAACATT TACATGTTGC AAATCAACCC AGACTCTTCA 
ATCAACCCTG ACCACTTGTC TTACTTCCAT TTCGTGGGTC GGATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATCAA TGGGGGTTTC ACGGTGCCTT TCTACAAGCA GCTACTGGGG 
AAGCCCATCC AGCTTTCTGA TTTGGAATCG GTGGACCCTG AACTCCATAA GAGCCTAGTG 
TGGATTCTAG AGAATGACAT CACCCCCGTG CTCGATCACA CGTTCTGCGT GGAGCACAAT 
GCTTTCGGGC GGATTCTTCA GCACGAACTG AAACCCAACG GCAGGAACGT CCCCGTCACA 
GAGGAGAATA AGAAGGAATA TGTCCGGTTG TATGTAAATT GGAGGTTCAT GAGGGGAATC 
GAGGCCCAGT TCCTGGCTCT TCAGAAGGGG TTTAACGAGC TCATCCCTCA ACACTTATTG 
AAGCCTTTTG ACCAGAAGGA GCTGGAGCTG ATCATTGGCG GCCTAGACAA GATCGACCTG 
GACGACTGGA AGTCCAACAC GCGGCTGAAG CACTGTGGGG CCGACAGCAA CGTGGTCCGG 
TGGTTCTGGC AGGCGGTAGA GACCTTCGAT GAGGAGCGGC GGGCCCGGCT GCTGCAGTTC 
GTGACAGGCT CCACGCGGGT CCCCCTCCAG GGCTTCAAGG CGCTGCAAGG CTCTACAGGC 
GCCGCAGGGC CCCGGCTCTT CACCATCCAC CTGATCGACG CCAACACGGA CAACCTGCCC 
AAGGCCCACA CCTGCTTTAA CCGGATCGAC ATCCCGCCCT ACGAGTCGTA CGAGAAGCTC 
TATGAGAAGC TGCTGACTGC GGTGGAGGAG ACCTGTGGCT TTGCCGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_591663.7 ) cDNA ORF clone, Bos taurus(cattle) -> XP_591663.5 Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa11955
Clone ID Related Accession (Same CDS sequence)	XM_002698145.5 , XM_591663.7
Accession Version	XM_591663.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646658.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_591663.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCTGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA ACTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGTCCC TGGGGGACCA CTCCTGGGGG AGATGGAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGCGTG 
AACTGCGATG AACTTGGACC ACTGCCGCCC GGTTGGGAAG TCCGCAGCAC GGTTTCCGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC GCGGTTACAC 
CACATCATGA CTCACCAGTG CCAACTGAAG GAGCCCAGCC AGCCACCACC GCCACCCAGC 
GAGGGCTCCG TGGAGGACGA GGAGCTGCCC GCCCAGAGGT ACGAGAGAGA TCTGGTCCAG 
AAACTGAAGG TCCTCAGACA CGAGCTCTCC TTGCAGCAGC CCCAGGCTGG CCACTGCCGC 
ATCGAGGTGT CCAGAGAGGA GATATTTGAG GAGTCTTATC GCCAGATAAT GAAGATGCGA 
CCAAAAGACT TGAAAAAGCG ACTCATGGTG AAATTCCGAG GAGAAGAAGG CTTGGACTAT 
GGAGGGGTGG CGAGGGAGTG GCTTTATTTA CTGTGCCATG AAATGCTGAA CCCGTACTAT 
GGACTCTTCC AGTACTCCAC GGACAACATT TACATGTTGC AAATCAACCC AGACTCTTCA 
ATCAACCCTG ACCACTTGTC TTACTTCCAT TTCGTGGGTC GGATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATCAA TGGGGGTTTC ACGGTGCCTT TCTACAAGCA GCTACTGGGG 
AAGCCCATCC AGCTTTCTGA TTTGGAATCG GTGGACCCTG AACTCCATAA GAGCCTAGTG 
TGGATTCTAG AGAATGACAT CACCCCCGTG CTCGATCACA CGTTCTGCGT GGAGCACAAT 
GCTTTCGGGC GGATTCTTCA GCACGAACTG AAACCCAACG GCAGGAACGT CCCCGTCACA 
GAGGAGAATA AGAAGGAATA TGTCCGGTTG TATGTAAATT GGAGGTTCAT GAGGGGAATC 
GAGGCCCAGT TCCTGGCTCT TCAGAAGGGG TTTAACGAGC TCATCCCTCA ACACTTATTG 
AAGCCTTTTG ACCAGAAGGA GCTGGAGCTG ATCATTGGCG GCCTAGACAA GATCGACCTG 
GACGACTGGA AGTCCAACAC GCGGCTGAAG CACTGTGGGG CCGACAGCAA CGTGGTCCGG 
TGGTTCTGGC AGGCGGTAGA GACCTTCGAT GAGGAGCGGC GGGCCCGGCT GCTGCAGTTC 
GTGACAGGCT CCACGCGGGT CCCCCTCCAG GGCTTCAAGG CGCTGCAAGG CTCTACAGGC 
GCCGCAGGGC CCCGGCTCTT CACCATCCAC CTGATCGACG CCAACACGGA CAACCTGCCC 
AAGGCCCACA CCTGCTTTAA CCGGATCGAC ATCCCGCCCT ACGAGTCGTA CGAGAAGCTC 
TATGAGAAGC TGCTGACTGC GGTGGAGGAG ACCTGTGGCT TTGCCGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_591663.5
CDS	77..2350
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLESEGTVYEDSGPGRPLSCFMEEPAPYTDGPGAAAGGGNCRFVESPSQDQRLQAQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPWGTTPGGDGAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMTHQCQLKEPSQPPPPPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_591663.7
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_591663.7

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCTGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA ACTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGTCCC TGGGGGACCA CTCCTGGGGG AGATGGAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGCGTG 
AACTGCGATG AACTTGGACC ACTGCCGCCC GGTTGGGAAG TCCGCAGCAC GGTTTCCGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC GCGGTTACAC 
CACATCATGA CTCACCAGTG CCAACTGAAG GAGCCCAGCC AGCCACCACC GCCACCCAGC 
GAGGGCTCCG TGGAGGACGA GGAGCTGCCC GCCCAGAGGT ACGAGAGAGA TCTGGTCCAG 
AAACTGAAGG TCCTCAGACA CGAGCTCTCC TTGCAGCAGC CCCAGGCTGG CCACTGCCGC 
ATCGAGGTGT CCAGAGAGGA GATATTTGAG GAGTCTTATC GCCAGATAAT GAAGATGCGA 
CCAAAAGACT TGAAAAAGCG ACTCATGGTG AAATTCCGAG GAGAAGAAGG CTTGGACTAT 
GGAGGGGTGG CGAGGGAGTG GCTTTATTTA CTGTGCCATG AAATGCTGAA CCCGTACTAT 
GGACTCTTCC AGTACTCCAC GGACAACATT TACATGTTGC AAATCAACCC AGACTCTTCA 
ATCAACCCTG ACCACTTGTC TTACTTCCAT TTCGTGGGTC GGATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATCAA TGGGGGTTTC ACGGTGCCTT TCTACAAGCA GCTACTGGGG 
AAGCCCATCC AGCTTTCTGA TTTGGAATCG GTGGACCCTG AACTCCATAA GAGCCTAGTG 
TGGATTCTAG AGAATGACAT CACCCCCGTG CTCGATCACA CGTTCTGCGT GGAGCACAAT 
GCTTTCGGGC GGATTCTTCA GCACGAACTG AAACCCAACG GCAGGAACGT CCCCGTCACA 
GAGGAGAATA AGAAGGAATA TGTCCGGTTG TATGTAAATT GGAGGTTCAT GAGGGGAATC 
GAGGCCCAGT TCCTGGCTCT TCAGAAGGGG TTTAACGAGC TCATCCCTCA ACACTTATTG 
AAGCCTTTTG ACCAGAAGGA GCTGGAGCTG ATCATTGGCG GCCTAGACAA GATCGACCTG 
GACGACTGGA AGTCCAACAC GCGGCTGAAG CACTGTGGGG CCGACAGCAA CGTGGTCCGG 
TGGTTCTGGC AGGCGGTAGA GACCTTCGAT GAGGAGCGGC GGGCCCGGCT GCTGCAGTTC 
GTGACAGGCT CCACGCGGGT CCCCCTCCAG GGCTTCAAGG CGCTGCAAGG CTCTACAGGC 
GCCGCAGGGC CCCGGCTCTT CACCATCCAC CTGATCGACG CCAACACGGA CAACCTGCCC 
AAGGCCCACA CCTGCTTTAA CCGGATCGAC ATCCCGCCCT ACGAGTCGTA CGAGAAGCTC 
TATGAGAAGC TGCTGACTGC GGTGGAGGAG ACCTGTGGCT TTGCCGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_005196999.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197056.1 Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa11956
Clone ID Related Accession (Same CDS sequence)	XM_005196999.3 , XM_005225271.3
Accession Version	XM_005196999.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646658.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196999.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCTGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA ACTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGAGAC CTTAACAGCG TGAACTGCGA TGAACTTGGA 
CCACTGCCGC CCGGTTGGGA AGTCCGCAGC ACGGTTTCCG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCGCGGTTAC ACCACATCAT GACTCACCAG 
TGCCAACTGA AGGAGCCCAG CCAGCCACCA CCGCCACCCA GCGAGGGCTC CGTGGAGGAC 
GAGGAGCTGC CCGCCCAGAG GTACGAGAGA GATCTGGTCC AGAAACTGAA GGTCCTCAGA 
CACGAGCTCT CCTTGCAGCA GCCCCAGGCT GGCCACTGCC GCATCGAGGT GTCCAGAGAG 
GAGATATTTG AGGAGTCTTA TCGCCAGATA ATGAAGATGC GACCAAAAGA CTTGAAAAAG 
CGACTCATGG TGAAATTCCG AGGAGAAGAA GGCTTGGACT ATGGAGGGGT GGCGAGGGAG 
TGGCTTTATT TACTGTGCCA TGAAATGCTG AACCCGTACT ATGGACTCTT CCAGTACTCC 
ACGGACAACA TTTACATGTT GCAAATCAAC CCAGACTCTT CAATCAACCC TGACCACTTG 
TCTTACTTCC ATTTCGTGGG TCGGATCATG GGTCTGGCTG TGTTCCACGG ACACTACATC 
AATGGGGGTT TCACGGTGCC TTTCTACAAG CAGCTACTGG GGAAGCCCAT CCAGCTTTCT 
GATTTGGAAT CGGTGGACCC TGAACTCCAT AAGAGCCTAG TGTGGATTCT AGAGAATGAC 
ATCACCCCCG TGCTCGATCA CACGTTCTGC GTGGAGCACA ATGCTTTCGG GCGGATTCTT 
CAGCACGAAC TGAAACCCAA CGGCAGGAAC GTCCCCGTCA CAGAGGAGAA TAAGAAGGAA 
TATGTCCGGT TGTATGTAAA TTGGAGGTTC ATGAGGGGAA TCGAGGCCCA GTTCCTGGCT 
CTTCAGAAGG GGTTTAACGA GCTCATCCCT CAACACTTAT TGAAGCCTTT TGACCAGAAG 
GAGCTGGAGC TGATCATTGG CGGCCTAGAC AAGATCGACC TGGACGACTG GAAGTCCAAC 
ACGCGGCTGA AGCACTGTGG GGCCGACAGC AACGTGGTCC GGTGGTTCTG GCAGGCGGTA 
GAGACCTTCG ATGAGGAGCG GCGGGCCCGG CTGCTGCAGT TCGTGACAGG CTCCACGCGG 
GTCCCCCTCC AGGGCTTCAA GGCGCTGCAA GGCTCTACAG GCGCCGCAGG GCCCCGGCTC 
TTCACCATCC ACCTGATCGA CGCCAACACG GACAACCTGC CCAAGGCCCA CACCTGCTTT 
AACCGGATCG ACATCCCGCC CTACGAGTCG TACGAGAAGC TCTATGAGAA GCTGCTGACT 
GCGGTGGAGG AGACCTGTGG CTTTGCCGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197056.1
CDS	30..2225
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLESEGTVYEDSGPGRPLSCFMEEPAPYTDGPGAAAGGGNCRFVESPSQDQRLQAQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMTHQCQLKEPSQPPPPPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_005196999.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196999.3

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCTGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA ACTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGAGAC CTTAACAGCG TGAACTGCGA TGAACTTGGA 
CCACTGCCGC CCGGTTGGGA AGTCCGCAGC ACGGTTTCCG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCGCGGTTAC ACCACATCAT GACTCACCAG 
TGCCAACTGA AGGAGCCCAG CCAGCCACCA CCGCCACCCA GCGAGGGCTC CGTGGAGGAC 
GAGGAGCTGC CCGCCCAGAG GTACGAGAGA GATCTGGTCC AGAAACTGAA GGTCCTCAGA 
CACGAGCTCT CCTTGCAGCA GCCCCAGGCT GGCCACTGCC GCATCGAGGT GTCCAGAGAG 
GAGATATTTG AGGAGTCTTA TCGCCAGATA ATGAAGATGC GACCAAAAGA CTTGAAAAAG 
CGACTCATGG TGAAATTCCG AGGAGAAGAA GGCTTGGACT ATGGAGGGGT GGCGAGGGAG 
TGGCTTTATT TACTGTGCCA TGAAATGCTG AACCCGTACT ATGGACTCTT CCAGTACTCC 
ACGGACAACA TTTACATGTT GCAAATCAAC CCAGACTCTT CAATCAACCC TGACCACTTG 
TCTTACTTCC ATTTCGTGGG TCGGATCATG GGTCTGGCTG TGTTCCACGG ACACTACATC 
AATGGGGGTT TCACGGTGCC TTTCTACAAG CAGCTACTGG GGAAGCCCAT CCAGCTTTCT 
GATTTGGAAT CGGTGGACCC TGAACTCCAT AAGAGCCTAG TGTGGATTCT AGAGAATGAC 
ATCACCCCCG TGCTCGATCA CACGTTCTGC GTGGAGCACA ATGCTTTCGG GCGGATTCTT 
CAGCACGAAC TGAAACCCAA CGGCAGGAAC GTCCCCGTCA CAGAGGAGAA TAAGAAGGAA 
TATGTCCGGT TGTATGTAAA TTGGAGGTTC ATGAGGGGAA TCGAGGCCCA GTTCCTGGCT 
CTTCAGAAGG GGTTTAACGA GCTCATCCCT CAACACTTAT TGAAGCCTTT TGACCAGAAG 
GAGCTGGAGC TGATCATTGG CGGCCTAGAC AAGATCGACC TGGACGACTG GAAGTCCAAC 
ACGCGGCTGA AGCACTGTGG GGCCGACAGC AACGTGGTCC GGTGGTTCTG GCAGGCGGTA 
GAGACCTTCG ATGAGGAGCG GCGGGCCCGG CTGCTGCAGT TCGTGACAGG CTCCACGCGG 
GTCCCCCTCC AGGGCTTCAA GGCGCTGCAA GGCTCTACAG GCGCCGCAGG GCCCCGGCTC 
TTCACCATCC ACCTGATCGA CGCCAACACG GACAACCTGC CCAAGGCCCA CACCTGCTTT 
AACCGGATCG ACATCCCGCC CTACGAGTCG TACGAGAAGC TCTATGAGAA GCTGCTGACT 
GCGGTGGAGG AGACCTGTGG CTTTGCCGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_005225271.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225328.1 Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa11956
Clone ID Related Accession (Same CDS sequence)	XM_005196999.3 , XM_005225271.3
Accession Version	XM_005225271.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225271.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCTGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA ACTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGAGAC CTTAACAGCG TGAACTGCGA TGAACTTGGA 
CCACTGCCGC CCGGTTGGGA AGTCCGCAGC ACGGTTTCCG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCGCGGTTAC ACCACATCAT GACTCACCAG 
TGCCAACTGA AGGAGCCCAG CCAGCCACCA CCGCCACCCA GCGAGGGCTC CGTGGAGGAC 
GAGGAGCTGC CCGCCCAGAG GTACGAGAGA GATCTGGTCC AGAAACTGAA GGTCCTCAGA 
CACGAGCTCT CCTTGCAGCA GCCCCAGGCT GGCCACTGCC GCATCGAGGT GTCCAGAGAG 
GAGATATTTG AGGAGTCTTA TCGCCAGATA ATGAAGATGC GACCAAAAGA CTTGAAAAAG 
CGACTCATGG TGAAATTCCG AGGAGAAGAA GGCTTGGACT ATGGAGGGGT GGCGAGGGAG 
TGGCTTTATT TACTGTGCCA TGAAATGCTG AACCCGTACT ATGGACTCTT CCAGTACTCC 
ACGGACAACA TTTACATGTT GCAAATCAAC CCAGACTCTT CAATCAACCC TGACCACTTG 
TCTTACTTCC ATTTCGTGGG TCGGATCATG GGTCTGGCTG TGTTCCACGG ACACTACATC 
AATGGGGGTT TCACGGTGCC TTTCTACAAG CAGCTACTGG GGAAGCCCAT CCAGCTTTCT 
GATTTGGAAT CGGTGGACCC TGAACTCCAT AAGAGCCTAG TGTGGATTCT AGAGAATGAC 
ATCACCCCCG TGCTCGATCA CACGTTCTGC GTGGAGCACA ATGCTTTCGG GCGGATTCTT 
CAGCACGAAC TGAAACCCAA CGGCAGGAAC GTCCCCGTCA CAGAGGAGAA TAAGAAGGAA 
TATGTCCGGT TGTATGTAAA TTGGAGGTTC ATGAGGGGAA TCGAGGCCCA GTTCCTGGCT 
CTTCAGAAGG GGTTTAACGA GCTCATCCCT CAACACTTAT TGAAGCCTTT TGACCAGAAG 
GAGCTGGAGC TGATCATTGG CGGCCTAGAC AAGATCGACC TGGACGACTG GAAGTCCAAC 
ACGCGGCTGA AGCACTGTGG GGCCGACAGC AACGTGGTCC GGTGGTTCTG GCAGGCGGTA 
GAGACCTTCG ATGAGGAGCG GCGGGCCCGG CTGCTGCAGT TCGTGACAGG CTCCACGCGG 
GTCCCCCTCC AGGGCTTCAA GGCGCTGCAA GGCTCTACAG GCGCCGCAGG GCCCCGGCTC 
TTCACCATCC ACCTGATCGA CGCCAACACG GACAACCTGC CCAAGGCCCA CACCTGCTTT 
AACCGGATCG ACATCCCGCC CTACGAGTCG TACGAGAAGC TCTATGAGAA GCTGCTGACT 
GCGGTGGAGG AGACCTGTGG CTTTGCCGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225328.1
CDS	30..2225
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLESEGTVYEDSGPGRPLSCFMEEPAPYTDGPGAAAGGGNCRFVESPSQDQRLQAQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMTHQCQLKEPSQPPPPPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_005225271.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225271.3

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCTGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA ACTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGAGAC CTTAACAGCG TGAACTGCGA TGAACTTGGA 
CCACTGCCGC CCGGTTGGGA AGTCCGCAGC ACGGTTTCCG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCGCGGTTAC ACCACATCAT GACTCACCAG 
TGCCAACTGA AGGAGCCCAG CCAGCCACCA CCGCCACCCA GCGAGGGCTC CGTGGAGGAC 
GAGGAGCTGC CCGCCCAGAG GTACGAGAGA GATCTGGTCC AGAAACTGAA GGTCCTCAGA 
CACGAGCTCT CCTTGCAGCA GCCCCAGGCT GGCCACTGCC GCATCGAGGT GTCCAGAGAG 
GAGATATTTG AGGAGTCTTA TCGCCAGATA ATGAAGATGC GACCAAAAGA CTTGAAAAAG 
CGACTCATGG TGAAATTCCG AGGAGAAGAA GGCTTGGACT ATGGAGGGGT GGCGAGGGAG 
TGGCTTTATT TACTGTGCCA TGAAATGCTG AACCCGTACT ATGGACTCTT CCAGTACTCC 
ACGGACAACA TTTACATGTT GCAAATCAAC CCAGACTCTT CAATCAACCC TGACCACTTG 
TCTTACTTCC ATTTCGTGGG TCGGATCATG GGTCTGGCTG TGTTCCACGG ACACTACATC 
AATGGGGGTT TCACGGTGCC TTTCTACAAG CAGCTACTGG GGAAGCCCAT CCAGCTTTCT 
GATTTGGAAT CGGTGGACCC TGAACTCCAT AAGAGCCTAG TGTGGATTCT AGAGAATGAC 
ATCACCCCCG TGCTCGATCA CACGTTCTGC GTGGAGCACA ATGCTTTCGG GCGGATTCTT 
CAGCACGAAC TGAAACCCAA CGGCAGGAAC GTCCCCGTCA CAGAGGAGAA TAAGAAGGAA 
TATGTCCGGT TGTATGTAAA TTGGAGGTTC ATGAGGGGAA TCGAGGCCCA GTTCCTGGCT 
CTTCAGAAGG GGTTTAACGA GCTCATCCCT CAACACTTAT TGAAGCCTTT TGACCAGAAG 
GAGCTGGAGC TGATCATTGG CGGCCTAGAC AAGATCGACC TGGACGACTG GAAGTCCAAC 
ACGCGGCTGA AGCACTGTGG GGCCGACAGC AACGTGGTCC GGTGGTTCTG GCAGGCGGTA 
GAGACCTTCG ATGAGGAGCG GCGGGCCCGG CTGCTGCAGT TCGTGACAGG CTCCACGCGG 
GTCCCCCTCC AGGGCTTCAA GGCGCTGCAA GGCTCTACAG GCGCCGCAGG GCCCCGGCTC 
TTCACCATCC ACCTGATCGA CGCCAACACG GACAACCTGC CCAAGGCCCA CACCTGCTTT 
AACCGGATCG ACATCCCGCC CTACGAGTCG TACGAGAAGC TCTATGAGAA GCTGCTGACT 
GCGGTGGAGG AGACCTGTGG CTTTGCCGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_005197000.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197057.1 Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa11957
Clone ID Related Accession (Same CDS sequence)	XM_005197000.3 , XM_005225272.3
Accession Version	XM_005197000.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646658.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197000.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCTGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA ACTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGAGAC CTTAACAGCG TGAACTGCGA TGAACTTGGA 
CCACTGCCGC CCGGTTGGGA AGTCCGCAGC ACGGTTTCCG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCGCGGTTAC ACCACATCAT GACTCACCAG 
TGCCAACTGA AGGAGCCCAG CCAGCCACCA CCGCCACCCA GCGAGGGCTC CGTGGAGGAC 
GAGGAGCTGC CCGCCCAGAG GTACGAGAGA GATCTGGTCC AGAAACTGAA GGTCCTCAGA 
CACGAGCTCT CCTTGCAGCA GCCCCAGGCT GGCCACTGCC GCATCGAGGT GTCCAGAGAG 
GAGATATTTG AGGAGTCTTA TCGCCAGATA ATGAAGATGC GACCAAAAGA CTTGAAAAAG 
CGACTCATGG TGAAATTCCG AGGAGAAGAA GGCTTGGACT ATGGAGGGGT GGCGAGGGAG 
TGGCTTTATT TACTGTGCCA TGAAATGCTG AACCCGTACT ATGGACTCTT CCAGTACTCC 
ACGGACAACA TTTACATGTT GCAAATCAAC CCAGACTCTT CAATCAACCC TGACCACTTG 
TCTTACTTCC ATTTCGTGGG TCGGATCATG GGTCTGGCTG TGTTCCACGG ACACTACATC 
AATGGGGGTT TCACGGTGCC TTTCTACAAG CAGCTACTGG GGAAGCCCAT CCAGCTTTCT 
GATTTGGAAT CGGTGGACCC TGAACTCCAT AAGAGCCTAG TGTGGATTCT AGAGAATGAC 
ATCACCCCCG TGCTCGATCA CACGTTCTGC GTGGAGCACA ATGCTTTCGG GCGGATTCTT 
CAGCACGAAC TGAAACCCAA CGGCAGGAAC GTCCCCGTCA CAGAGGAGAA TAAGAAGGAA 
TATGTCCGGT TGTATGTAAA TTGGAGGTTC ATGAGGGGAA TCGAGGCCCA GTTCCTGGCT 
CTTCAGAAGG GGTTTAACGA GCTCATCCCT CAACACTTAT TGAAGCCTTT TGACCAGAAG 
GAGCTGGAGC TGATCATTGG CGGCCTAGAC AAGATCGACC TGGACGACTG GAAGTCCAAC 
ACGCGGCTGA AGCACTGTGG GGCCGACAGC AACGTGGTCC GGTGGTTCTG GCAGGCGGTA 
GAGACCTTCG ATGAGGAGCG GCGGGCCCGG CTGCTGCAGT TCGTGACAGG CTCCACGCGG 
GTCCCCCTCC AGGGCTTCAA GGCGCTGCAA GGCGCCGCAG GGCCCCGGCT CTTCACCATC 
CACCTGATCG ACGCCAACAC GGACAACCTG CCCAAGGCCC ACACCTGCTT TAACCGGATC 
GACATCCCGC CCTACGAGTC GTACGAGAAG CTCTATGAGA AGCTGCTGAC TGCGGTGGAG 
GAGACCTGTG GCTTTGCCGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197057.1
CDS	74..2260
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLESEGTVYEDSGPGRPLSCFMEEPAPYTDGPGAAAGGGNCRFVESPSQDQRLQAQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMTHQCQLKEPSQPPPPPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_005197000.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197000.3

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCTGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA ACTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGAGAC CTTAACAGCG TGAACTGCGA TGAACTTGGA 
CCACTGCCGC CCGGTTGGGA AGTCCGCAGC ACGGTTTCCG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCGCGGTTAC ACCACATCAT GACTCACCAG 
TGCCAACTGA AGGAGCCCAG CCAGCCACCA CCGCCACCCA GCGAGGGCTC CGTGGAGGAC 
GAGGAGCTGC CCGCCCAGAG GTACGAGAGA GATCTGGTCC AGAAACTGAA GGTCCTCAGA 
CACGAGCTCT CCTTGCAGCA GCCCCAGGCT GGCCACTGCC GCATCGAGGT GTCCAGAGAG 
GAGATATTTG AGGAGTCTTA TCGCCAGATA ATGAAGATGC GACCAAAAGA CTTGAAAAAG 
CGACTCATGG TGAAATTCCG AGGAGAAGAA GGCTTGGACT ATGGAGGGGT GGCGAGGGAG 
TGGCTTTATT TACTGTGCCA TGAAATGCTG AACCCGTACT ATGGACTCTT CCAGTACTCC 
ACGGACAACA TTTACATGTT GCAAATCAAC CCAGACTCTT CAATCAACCC TGACCACTTG 
TCTTACTTCC ATTTCGTGGG TCGGATCATG GGTCTGGCTG TGTTCCACGG ACACTACATC 
AATGGGGGTT TCACGGTGCC TTTCTACAAG CAGCTACTGG GGAAGCCCAT CCAGCTTTCT 
GATTTGGAAT CGGTGGACCC TGAACTCCAT AAGAGCCTAG TGTGGATTCT AGAGAATGAC 
ATCACCCCCG TGCTCGATCA CACGTTCTGC GTGGAGCACA ATGCTTTCGG GCGGATTCTT 
CAGCACGAAC TGAAACCCAA CGGCAGGAAC GTCCCCGTCA CAGAGGAGAA TAAGAAGGAA 
TATGTCCGGT TGTATGTAAA TTGGAGGTTC ATGAGGGGAA TCGAGGCCCA GTTCCTGGCT 
CTTCAGAAGG GGTTTAACGA GCTCATCCCT CAACACTTAT TGAAGCCTTT TGACCAGAAG 
GAGCTGGAGC TGATCATTGG CGGCCTAGAC AAGATCGACC TGGACGACTG GAAGTCCAAC 
ACGCGGCTGA AGCACTGTGG GGCCGACAGC AACGTGGTCC GGTGGTTCTG GCAGGCGGTA 
GAGACCTTCG ATGAGGAGCG GCGGGCCCGG CTGCTGCAGT TCGTGACAGG CTCCACGCGG 
GTCCCCCTCC AGGGCTTCAA GGCGCTGCAA GGCGCCGCAG GGCCCCGGCT CTTCACCATC 
CACCTGATCG ACGCCAACAC GGACAACCTG CCCAAGGCCC ACACCTGCTT TAACCGGATC 
GACATCCCGC CCTACGAGTC GTACGAGAAG CTCTATGAGA AGCTGCTGAC TGCGGTGGAG 
GAGACCTGTG GCTTTGCCGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_005225272.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225329.1 Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

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CloneID	OBa11957
Clone ID Related Accession (Same CDS sequence)	XM_005197000.3 , XM_005225272.3
Accession Version	XM_005225272.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225272.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCTGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA ACTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGAGAC CTTAACAGCG TGAACTGCGA TGAACTTGGA 
CCACTGCCGC CCGGTTGGGA AGTCCGCAGC ACGGTTTCCG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCGCGGTTAC ACCACATCAT GACTCACCAG 
TGCCAACTGA AGGAGCCCAG CCAGCCACCA CCGCCACCCA GCGAGGGCTC CGTGGAGGAC 
GAGGAGCTGC CCGCCCAGAG GTACGAGAGA GATCTGGTCC AGAAACTGAA GGTCCTCAGA 
CACGAGCTCT CCTTGCAGCA GCCCCAGGCT GGCCACTGCC GCATCGAGGT GTCCAGAGAG 
GAGATATTTG AGGAGTCTTA TCGCCAGATA ATGAAGATGC GACCAAAAGA CTTGAAAAAG 
CGACTCATGG TGAAATTCCG AGGAGAAGAA GGCTTGGACT ATGGAGGGGT GGCGAGGGAG 
TGGCTTTATT TACTGTGCCA TGAAATGCTG AACCCGTACT ATGGACTCTT CCAGTACTCC 
ACGGACAACA TTTACATGTT GCAAATCAAC CCAGACTCTT CAATCAACCC TGACCACTTG 
TCTTACTTCC ATTTCGTGGG TCGGATCATG GGTCTGGCTG TGTTCCACGG ACACTACATC 
AATGGGGGTT TCACGGTGCC TTTCTACAAG CAGCTACTGG GGAAGCCCAT CCAGCTTTCT 
GATTTGGAAT CGGTGGACCC TGAACTCCAT AAGAGCCTAG TGTGGATTCT AGAGAATGAC 
ATCACCCCCG TGCTCGATCA CACGTTCTGC GTGGAGCACA ATGCTTTCGG GCGGATTCTT 
CAGCACGAAC TGAAACCCAA CGGCAGGAAC GTCCCCGTCA CAGAGGAGAA TAAGAAGGAA 
TATGTCCGGT TGTATGTAAA TTGGAGGTTC ATGAGGGGAA TCGAGGCCCA GTTCCTGGCT 
CTTCAGAAGG GGTTTAACGA GCTCATCCCT CAACACTTAT TGAAGCCTTT TGACCAGAAG 
GAGCTGGAGC TGATCATTGG CGGCCTAGAC AAGATCGACC TGGACGACTG GAAGTCCAAC 
ACGCGGCTGA AGCACTGTGG GGCCGACAGC AACGTGGTCC GGTGGTTCTG GCAGGCGGTA 
GAGACCTTCG ATGAGGAGCG GCGGGCCCGG CTGCTGCAGT TCGTGACAGG CTCCACGCGG 
GTCCCCCTCC AGGGCTTCAA GGCGCTGCAA GGCGCCGCAG GGCCCCGGCT CTTCACCATC 
CACCTGATCG ACGCCAACAC GGACAACCTG CCCAAGGCCC ACACCTGCTT TAACCGGATC 
GACATCCCGC CCTACGAGTC GTACGAGAAG CTCTATGAGA AGCTGCTGAC TGCGGTGGAG 
GAGACCTGTG GCTTTGCCGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225329.1
CDS	74..2260
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLESEGTVYEDSGPGRPLSCFMEEPAPYTDGPGAAAGGGNCRFVESPSQDQRLQAQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMTHQCQLKEPSQPPPPPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_005225272.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225272.3

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCTGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA ACTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGAGAC CTTAACAGCG TGAACTGCGA TGAACTTGGA 
CCACTGCCGC CCGGTTGGGA AGTCCGCAGC ACGGTTTCCG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCGCGGTTAC ACCACATCAT GACTCACCAG 
TGCCAACTGA AGGAGCCCAG CCAGCCACCA CCGCCACCCA GCGAGGGCTC CGTGGAGGAC 
GAGGAGCTGC CCGCCCAGAG GTACGAGAGA GATCTGGTCC AGAAACTGAA GGTCCTCAGA 
CACGAGCTCT CCTTGCAGCA GCCCCAGGCT GGCCACTGCC GCATCGAGGT GTCCAGAGAG 
GAGATATTTG AGGAGTCTTA TCGCCAGATA ATGAAGATGC GACCAAAAGA CTTGAAAAAG 
CGACTCATGG TGAAATTCCG AGGAGAAGAA GGCTTGGACT ATGGAGGGGT GGCGAGGGAG 
TGGCTTTATT TACTGTGCCA TGAAATGCTG AACCCGTACT ATGGACTCTT CCAGTACTCC 
ACGGACAACA TTTACATGTT GCAAATCAAC CCAGACTCTT CAATCAACCC TGACCACTTG 
TCTTACTTCC ATTTCGTGGG TCGGATCATG GGTCTGGCTG TGTTCCACGG ACACTACATC 
AATGGGGGTT TCACGGTGCC TTTCTACAAG CAGCTACTGG GGAAGCCCAT CCAGCTTTCT 
GATTTGGAAT CGGTGGACCC TGAACTCCAT AAGAGCCTAG TGTGGATTCT AGAGAATGAC 
ATCACCCCCG TGCTCGATCA CACGTTCTGC GTGGAGCACA ATGCTTTCGG GCGGATTCTT 
CAGCACGAAC TGAAACCCAA CGGCAGGAAC GTCCCCGTCA CAGAGGAGAA TAAGAAGGAA 
TATGTCCGGT TGTATGTAAA TTGGAGGTTC ATGAGGGGAA TCGAGGCCCA GTTCCTGGCT 
CTTCAGAAGG GGTTTAACGA GCTCATCCCT CAACACTTAT TGAAGCCTTT TGACCAGAAG 
GAGCTGGAGC TGATCATTGG CGGCCTAGAC AAGATCGACC TGGACGACTG GAAGTCCAAC 
ACGCGGCTGA AGCACTGTGG GGCCGACAGC AACGTGGTCC GGTGGTTCTG GCAGGCGGTA 
GAGACCTTCG ATGAGGAGCG GCGGGCCCGG CTGCTGCAGT TCGTGACAGG CTCCACGCGG 
GTCCCCCTCC AGGGCTTCAA GGCGCTGCAA GGCGCCGCAG GGCCCCGGCT CTTCACCATC 
CACCTGATCG ACGCCAACAC GGACAACCTG CCCAAGGCCC ACACCTGCTT TAACCGGATC 
GACATCCCGC CCTACGAGTC GTACGAGAAG CTCTATGAGA AGCTGCTGAC TGCGGTGGAG 
GAGACCTGTG GCTTTGCCGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_010819648.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817950.1 Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

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CloneID	OBa48766
Clone ID Related Accession (Same CDS sequence)	XM_010819648.2 , XM_010825852.2
Accession Version	XM_010819648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTGT CTCCTAGGTG GCTCTGTAGT GCCACCTTCA AGGTATTATG TGCCAAGAAC 
CTTGCAAAGA AAGACTTCTT CAGGCTCCCT GACCCTTTTG CAAAGATTGT TGTGGACGGA 
TCTGGGCAGT GCCACTCAAC GGACACGGTG AAGAACACAC TGGACCCAAA GTGGAACCAG 
CACTACGATC TATATGTTGG AAAAACGGAT TCTATAACCA TCAGCGTGTG GAACCACAAG 
AAGATTCACA AGAAGCAGGG AGCCGGCTTC CTGGGGTGTG TGCGGCTGCT GTCCAACGCC 
ATCAGCAGAT TAAAAGATAC TGGCTACCAG CGTTTGGATC TGTGCAAACT AAACCCCTCA 
GATACTGATG CAGTTCGTGG CCAAATAGTG GTCAGTTTAC AGACACGAGA CAGGATCGGC 
ACTGGCGGCT CTGTGGTGGA CTGCAGAGGC CTGCTGGAGA GCGAAGGAAC CGTGTATGAA 
GACTCTGGGC CTGGAAGGCC CCTCAGCTGC TTCATGGAGG AGCCAGCCCC GTACACGGAT 
GGCCCCGGGG CCGCTGCTGG CGGGGGCAAC TGCAGGTTTG TGGAATCCCC GAGTCAAGAC 
CAAAGACTTC AGGCCCAGCG ACTGCGAAAC CCTGAGGTGC GAGGGTCGCT CCAGACGCCA 
CAGAACCGAC CTCACGGCCA CCAGTCCCCG GAGCTGCCCG AAGGCTACGA GCAGAGAACC 
ACGGTACAGG GCCAGGTGTA CTTCCTGCAC ACGCAGACGG GAGTCAGCAC GTGGCACGAC 
CCCAGGATAC CCAGAGACCT TAACAGCGTG AACTGCGATG AACTTGGACC ACTGCCGCCC 
GGTTGGGAAG TCCGCAGCAC GGTTTCCGGG AGGATATATT TTGTAGATCA TAATAACCGA 
ACAACCCAGT TTACAGACCC GCGGTTACAC CACATCATGA CTCACCAGTG CCAACTGAAG 
GAGCCCAGCC AGCCACCACC GCCACCCAGC GAGGGCTCCG TGGAGGACGA GGAGCTGCCC 
GCCCAGAGGT ACGAGAGAGA TCTGGTCCAG AAACTGAAGG TCCTCAGACA CGAGCTCTCC 
TTGCAGCAGC CCCAGGCTGG CCACTGCCGC ATCGAGGTGT CCAGAGAGGA GATATTTGAG 
GAGTCTTATC GCCAGATAAT GAAGATGCGA CCAAAAGACT TGAAAAAGCG ACTCATGGTG 
AAATTCCGAG GAGAAGAAGG CTTGGACTAT GGAGGGGTGG CGAGGGAGTG GCTTTATTTA 
CTGTGCCATG AAATGCTGAA CCCGTACTAT GGACTCTTCC AGTACTCCAC GGACAACATT 
TACATGTTGC AAATCAACCC AGACTCTTCA ATCAACCCTG ACCACTTGTC TTACTTCCAT 
TTCGTGGGTC GGATCATGGG TCTGGCTGTG TTCCACGGAC ACTACATCAA TGGGGGTTTC 
ACGGTGCCTT TCTACAAGCA GCTACTGGGG AAGCCCATCC AGCTTTCTGA TTTGGAATCG 
GTGGACCCTG AACTCCATAA GAGCCTAGTG TGGATTCTAG AGAATGACAT CACCCCCGTG 
CTCGATCACA CGTTCTGCGT GGAGCACAAT GCTTTCGGGC GGATTCTTCA GCACGAACTG 
AAACCCAACG GCAGGAACGT CCCCGTCACA GAGGAGAATA AGAAGGAATA TGTCCGGTTG 
TATGTAAATT GGAGGTTCAT GAGGGGAATC GAGGCCCAGT TCCTGGCTCT TCAGAAGGGG 
TTTAACGAGC TCATCCCTCA ACACTTATTG AAGCCTTTTG ACCAGAAGGA GCTGGAGCTG 
ATCATTGGCG GCCTAGACAA GATCGACCTG GACGACTGGA AGTCCAACAC GCGGCTGAAG 
CACTGTGGGG CCGACAGCAA CGTGGTCCGG TGGTTCTGGC AGGCGGTAGA GACCTTCGAT 
GAGGAGCGGC GGGCCCGGCT GCTGCAGTTC GTGACAGGCT CCACGCGGGT CCCCCTCCAG 
GGCTTCAAGG CGCTGCAAGG CTCTACAGGC GCCGCAGGGC CCCGGCTCTT CACCATCCAC 
CTGATCGACG CCAACACGGA CAACCTGCCC AAGGCCCACA CCTGCTTTAA CCGGATCGAC 
ATCCCGCCCT ACGAGTCGTA CGAGAAGCTC TATGAGAAGC TGCTGACTGC GGTGGAGGAG 
ACCTGTGGCT TTGCCGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817950.1
CDS	413..2596
Translation	MPVSPRWLCSATFKVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLESEGTVYEDSGPGRPLSCFMEEPAPYTDGPGAAAGGGNCRFVESPSQDQRLQAQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMTHQCQLKEPSQPPPPPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_010819648.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819648.2

ORF Insert Sequence:

ATGCCCGTGT CTCCTAGGTG GCTCTGTAGT GCCACCTTCA AGGTATTATG TGCCAAGAAC 
CTTGCAAAGA AAGACTTCTT CAGGCTCCCT GACCCTTTTG CAAAGATTGT TGTGGACGGA 
TCTGGGCAGT GCCACTCAAC GGACACGGTG AAGAACACAC TGGACCCAAA GTGGAACCAG 
CACTACGATC TATATGTTGG AAAAACGGAT TCTATAACCA TCAGCGTGTG GAACCACAAG 
AAGATTCACA AGAAGCAGGG AGCCGGCTTC CTGGGGTGTG TGCGGCTGCT GTCCAACGCC 
ATCAGCAGAT TAAAAGATAC TGGCTACCAG CGTTTGGATC TGTGCAAACT AAACCCCTCA 
GATACTGATG CAGTTCGTGG CCAAATAGTG GTCAGTTTAC AGACACGAGA CAGGATCGGC 
ACTGGCGGCT CTGTGGTGGA CTGCAGAGGC CTGCTGGAGA GCGAAGGAAC CGTGTATGAA 
GACTCTGGGC CTGGAAGGCC CCTCAGCTGC TTCATGGAGG AGCCAGCCCC GTACACGGAT 
GGCCCCGGGG CCGCTGCTGG CGGGGGCAAC TGCAGGTTTG TGGAATCCCC GAGTCAAGAC 
CAAAGACTTC AGGCCCAGCG ACTGCGAAAC CCTGAGGTGC GAGGGTCGCT CCAGACGCCA 
CAGAACCGAC CTCACGGCCA CCAGTCCCCG GAGCTGCCCG AAGGCTACGA GCAGAGAACC 
ACGGTACAGG GCCAGGTGTA CTTCCTGCAC ACGCAGACGG GAGTCAGCAC GTGGCACGAC 
CCCAGGATAC CCAGAGACCT TAACAGCGTG AACTGCGATG AACTTGGACC ACTGCCGCCC 
GGTTGGGAAG TCCGCAGCAC GGTTTCCGGG AGGATATATT TTGTAGATCA TAATAACCGA 
ACAACCCAGT TTACAGACCC GCGGTTACAC CACATCATGA CTCACCAGTG CCAACTGAAG 
GAGCCCAGCC AGCCACCACC GCCACCCAGC GAGGGCTCCG TGGAGGACGA GGAGCTGCCC 
GCCCAGAGGT ACGAGAGAGA TCTGGTCCAG AAACTGAAGG TCCTCAGACA CGAGCTCTCC 
TTGCAGCAGC CCCAGGCTGG CCACTGCCGC ATCGAGGTGT CCAGAGAGGA GATATTTGAG 
GAGTCTTATC GCCAGATAAT GAAGATGCGA CCAAAAGACT TGAAAAAGCG ACTCATGGTG 
AAATTCCGAG GAGAAGAAGG CTTGGACTAT GGAGGGGTGG CGAGGGAGTG GCTTTATTTA 
CTGTGCCATG AAATGCTGAA CCCGTACTAT GGACTCTTCC AGTACTCCAC GGACAACATT 
TACATGTTGC AAATCAACCC AGACTCTTCA ATCAACCCTG ACCACTTGTC TTACTTCCAT 
TTCGTGGGTC GGATCATGGG TCTGGCTGTG TTCCACGGAC ACTACATCAA TGGGGGTTTC 
ACGGTGCCTT TCTACAAGCA GCTACTGGGG AAGCCCATCC AGCTTTCTGA TTTGGAATCG 
GTGGACCCTG AACTCCATAA GAGCCTAGTG TGGATTCTAG AGAATGACAT CACCCCCGTG 
CTCGATCACA CGTTCTGCGT GGAGCACAAT GCTTTCGGGC GGATTCTTCA GCACGAACTG 
AAACCCAACG GCAGGAACGT CCCCGTCACA GAGGAGAATA AGAAGGAATA TGTCCGGTTG 
TATGTAAATT GGAGGTTCAT GAGGGGAATC GAGGCCCAGT TCCTGGCTCT TCAGAAGGGG 
TTTAACGAGC TCATCCCTCA ACACTTATTG AAGCCTTTTG ACCAGAAGGA GCTGGAGCTG 
ATCATTGGCG GCCTAGACAA GATCGACCTG GACGACTGGA AGTCCAACAC GCGGCTGAAG 
CACTGTGGGG CCGACAGCAA CGTGGTCCGG TGGTTCTGGC AGGCGGTAGA GACCTTCGAT 
GAGGAGCGGC GGGCCCGGCT GCTGCAGTTC GTGACAGGCT CCACGCGGGT CCCCCTCCAG 
GGCTTCAAGG CGCTGCAAGG CTCTACAGGC GCCGCAGGGC CCCGGCTCTT CACCATCCAC 
CTGATCGACG CCAACACGGA CAACCTGCCC AAGGCCCACA CCTGCTTTAA CCGGATCGAC 
ATCCCGCCCT ACGAGTCGTA CGAGAAGCTC TATGAGAAGC TGCTGACTGC GGTGGAGGAG 
ACCTGTGGCT TTGCCGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_010825852.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824154.1 Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

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CloneID	OBa48766
Clone ID Related Accession (Same CDS sequence)	XM_010819648.2 , XM_010825852.2
Accession Version	XM_010825852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646658.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTGT CTCCTAGGTG GCTCTGTAGT GCCACCTTCA AGGTATTATG TGCCAAGAAC 
CTTGCAAAGA AAGACTTCTT CAGGCTCCCT GACCCTTTTG CAAAGATTGT TGTGGACGGA 
TCTGGGCAGT GCCACTCAAC GGACACGGTG AAGAACACAC TGGACCCAAA GTGGAACCAG 
CACTACGATC TATATGTTGG AAAAACGGAT TCTATAACCA TCAGCGTGTG GAACCACAAG 
AAGATTCACA AGAAGCAGGG AGCCGGCTTC CTGGGGTGTG TGCGGCTGCT GTCCAACGCC 
ATCAGCAGAT TAAAAGATAC TGGCTACCAG CGTTTGGATC TGTGCAAACT AAACCCCTCA 
GATACTGATG CAGTTCGTGG CCAAATAGTG GTCAGTTTAC AGACACGAGA CAGGATCGGC 
ACTGGCGGCT CTGTGGTGGA CTGCAGAGGC CTGCTGGAGA GCGAAGGAAC CGTGTATGAA 
GACTCTGGGC CTGGAAGGCC CCTCAGCTGC TTCATGGAGG AGCCAGCCCC GTACACGGAT 
GGCCCCGGGG CCGCTGCTGG CGGGGGCAAC TGCAGGTTTG TGGAATCCCC GAGTCAAGAC 
CAAAGACTTC AGGCCCAGCG ACTGCGAAAC CCTGAGGTGC GAGGGTCGCT CCAGACGCCA 
CAGAACCGAC CTCACGGCCA CCAGTCCCCG GAGCTGCCCG AAGGCTACGA GCAGAGAACC 
ACGGTACAGG GCCAGGTGTA CTTCCTGCAC ACGCAGACGG GAGTCAGCAC GTGGCACGAC 
CCCAGGATAC CCAGAGACCT TAACAGCGTG AACTGCGATG AACTTGGACC ACTGCCGCCC 
GGTTGGGAAG TCCGCAGCAC GGTTTCCGGG AGGATATATT TTGTAGATCA TAATAACCGA 
ACAACCCAGT TTACAGACCC GCGGTTACAC CACATCATGA CTCACCAGTG CCAACTGAAG 
GAGCCCAGCC AGCCACCACC GCCACCCAGC GAGGGCTCCG TGGAGGACGA GGAGCTGCCC 
GCCCAGAGGT ACGAGAGAGA TCTGGTCCAG AAACTGAAGG TCCTCAGACA CGAGCTCTCC 
TTGCAGCAGC CCCAGGCTGG CCACTGCCGC ATCGAGGTGT CCAGAGAGGA GATATTTGAG 
GAGTCTTATC GCCAGATAAT GAAGATGCGA CCAAAAGACT TGAAAAAGCG ACTCATGGTG 
AAATTCCGAG GAGAAGAAGG CTTGGACTAT GGAGGGGTGG CGAGGGAGTG GCTTTATTTA 
CTGTGCCATG AAATGCTGAA CCCGTACTAT GGACTCTTCC AGTACTCCAC GGACAACATT 
TACATGTTGC AAATCAACCC AGACTCTTCA ATCAACCCTG ACCACTTGTC TTACTTCCAT 
TTCGTGGGTC GGATCATGGG TCTGGCTGTG TTCCACGGAC ACTACATCAA TGGGGGTTTC 
ACGGTGCCTT TCTACAAGCA GCTACTGGGG AAGCCCATCC AGCTTTCTGA TTTGGAATCG 
GTGGACCCTG AACTCCATAA GAGCCTAGTG TGGATTCTAG AGAATGACAT CACCCCCGTG 
CTCGATCACA CGTTCTGCGT GGAGCACAAT GCTTTCGGGC GGATTCTTCA GCACGAACTG 
AAACCCAACG GCAGGAACGT CCCCGTCACA GAGGAGAATA AGAAGGAATA TGTCCGGTTG 
TATGTAAATT GGAGGTTCAT GAGGGGAATC GAGGCCCAGT TCCTGGCTCT TCAGAAGGGG 
TTTAACGAGC TCATCCCTCA ACACTTATTG AAGCCTTTTG ACCAGAAGGA GCTGGAGCTG 
ATCATTGGCG GCCTAGACAA GATCGACCTG GACGACTGGA AGTCCAACAC GCGGCTGAAG 
CACTGTGGGG CCGACAGCAA CGTGGTCCGG TGGTTCTGGC AGGCGGTAGA GACCTTCGAT 
GAGGAGCGGC GGGCCCGGCT GCTGCAGTTC GTGACAGGCT CCACGCGGGT CCCCCTCCAG 
GGCTTCAAGG CGCTGCAAGG CTCTACAGGC GCCGCAGGGC CCCGGCTCTT CACCATCCAC 
CTGATCGACG CCAACACGGA CAACCTGCCC AAGGCCCACA CCTGCTTTAA CCGGATCGAC 
ATCCCGCCCT ACGAGTCGTA CGAGAAGCTC TATGAGAAGC TGCTGACTGC GGTGGAGGAG 
ACCTGTGGCT TTGCCGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824154.1
CDS	413..2596
Translation	MPVSPRWLCSATFKVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLESEGTVYEDSGPGRPLSCFMEEPAPYTDGPGAAAGGGNCRFVESPSQDQRLQAQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMTHQCQLKEPSQPPPPPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_010825852.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825852.2

ORF Insert Sequence:

ATGCCCGTGT CTCCTAGGTG GCTCTGTAGT GCCACCTTCA AGGTATTATG TGCCAAGAAC 
CTTGCAAAGA AAGACTTCTT CAGGCTCCCT GACCCTTTTG CAAAGATTGT TGTGGACGGA 
TCTGGGCAGT GCCACTCAAC GGACACGGTG AAGAACACAC TGGACCCAAA GTGGAACCAG 
CACTACGATC TATATGTTGG AAAAACGGAT TCTATAACCA TCAGCGTGTG GAACCACAAG 
AAGATTCACA AGAAGCAGGG AGCCGGCTTC CTGGGGTGTG TGCGGCTGCT GTCCAACGCC 
ATCAGCAGAT TAAAAGATAC TGGCTACCAG CGTTTGGATC TGTGCAAACT AAACCCCTCA 
GATACTGATG CAGTTCGTGG CCAAATAGTG GTCAGTTTAC AGACACGAGA CAGGATCGGC 
ACTGGCGGCT CTGTGGTGGA CTGCAGAGGC CTGCTGGAGA GCGAAGGAAC CGTGTATGAA 
GACTCTGGGC CTGGAAGGCC CCTCAGCTGC TTCATGGAGG AGCCAGCCCC GTACACGGAT 
GGCCCCGGGG CCGCTGCTGG CGGGGGCAAC TGCAGGTTTG TGGAATCCCC GAGTCAAGAC 
CAAAGACTTC AGGCCCAGCG ACTGCGAAAC CCTGAGGTGC GAGGGTCGCT CCAGACGCCA 
CAGAACCGAC CTCACGGCCA CCAGTCCCCG GAGCTGCCCG AAGGCTACGA GCAGAGAACC 
ACGGTACAGG GCCAGGTGTA CTTCCTGCAC ACGCAGACGG GAGTCAGCAC GTGGCACGAC 
CCCAGGATAC CCAGAGACCT TAACAGCGTG AACTGCGATG AACTTGGACC ACTGCCGCCC 
GGTTGGGAAG TCCGCAGCAC GGTTTCCGGG AGGATATATT TTGTAGATCA TAATAACCGA 
ACAACCCAGT TTACAGACCC GCGGTTACAC CACATCATGA CTCACCAGTG CCAACTGAAG 
GAGCCCAGCC AGCCACCACC GCCACCCAGC GAGGGCTCCG TGGAGGACGA GGAGCTGCCC 
GCCCAGAGGT ACGAGAGAGA TCTGGTCCAG AAACTGAAGG TCCTCAGACA CGAGCTCTCC 
TTGCAGCAGC CCCAGGCTGG CCACTGCCGC ATCGAGGTGT CCAGAGAGGA GATATTTGAG 
GAGTCTTATC GCCAGATAAT GAAGATGCGA CCAAAAGACT TGAAAAAGCG ACTCATGGTG 
AAATTCCGAG GAGAAGAAGG CTTGGACTAT GGAGGGGTGG CGAGGGAGTG GCTTTATTTA 
CTGTGCCATG AAATGCTGAA CCCGTACTAT GGACTCTTCC AGTACTCCAC GGACAACATT 
TACATGTTGC AAATCAACCC AGACTCTTCA ATCAACCCTG ACCACTTGTC TTACTTCCAT 
TTCGTGGGTC GGATCATGGG TCTGGCTGTG TTCCACGGAC ACTACATCAA TGGGGGTTTC 
ACGGTGCCTT TCTACAAGCA GCTACTGGGG AAGCCCATCC AGCTTTCTGA TTTGGAATCG 
GTGGACCCTG AACTCCATAA GAGCCTAGTG TGGATTCTAG AGAATGACAT CACCCCCGTG 
CTCGATCACA CGTTCTGCGT GGAGCACAAT GCTTTCGGGC GGATTCTTCA GCACGAACTG 
AAACCCAACG GCAGGAACGT CCCCGTCACA GAGGAGAATA AGAAGGAATA TGTCCGGTTG 
TATGTAAATT GGAGGTTCAT GAGGGGAATC GAGGCCCAGT TCCTGGCTCT TCAGAAGGGG 
TTTAACGAGC TCATCCCTCA ACACTTATTG AAGCCTTTTG ACCAGAAGGA GCTGGAGCTG 
ATCATTGGCG GCCTAGACAA GATCGACCTG GACGACTGGA AGTCCAACAC GCGGCTGAAG 
CACTGTGGGG CCGACAGCAA CGTGGTCCGG TGGTTCTGGC AGGCGGTAGA GACCTTCGAT 
GAGGAGCGGC GGGCCCGGCT GCTGCAGTTC GTGACAGGCT CCACGCGGGT CCCCCTCCAG 
GGCTTCAAGG CGCTGCAAGG CTCTACAGGC GCCGCAGGGC CCCGGCTCTT CACCATCCAC 
CTGATCGACG CCAACACGGA CAACCTGCCC AAGGCCCACA CCTGCTTTAA CCGGATCGAC 
ATCCCGCCCT ACGAGTCGTA CGAGAAGCTC TATGAGAAGC TGCTGACTGC GGTGGAGGAG 
ACCTGTGGCT TTGCCGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_005225271.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225328.2 Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa230703
Clone ID Related Accession (Same CDS sequence)	XM_005225271.4
Accession Version	XM_005225271.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225271.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCCGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA CCTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGAGAC CTTAACAGCG TGAACTGCGA TGAACTTGGA 
CCACTGCCGC CCGGTTGGGA AGTCCGCAGC ACGGTTTCCG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCGCGGTTAC ACCACATCAT GACTCACCAG 
TGCCAACTGA AGGAGCCCAG CCAGCCACCA CCGCCACCCA GCGAGGGCTC CGTGGAGGAC 
GAGGAGCTGC CCGCCCAGAG GTACGAGAGA GATCTGGTCC AGAAACTGAA GGTCCTCAGA 
CACGAGCTCT CCTTGCAGCA GCCCCAGGCT GGCCACTGCC GCATCGAGGT GTCCAGAGAG 
GAGATATTTG AGGAGTCTTA TCGCCAGATA ATGAAGATGC GACCAAAAGA CTTGAAAAAG 
CGACTCATGG TGAAATTCCG AGGAGAAGAA GGCTTGGACT ATGGAGGGGT GGCGAGGGAG 
TGGCTTTATT TACTGTGCCA TGAAATGTTG AACCCGTACT ATGGACTCTT CCAGTACTCC 
ACGGACAACA TTTACATGTT GCAAATCAAC CCAGACTCTT CAATCAACCC TGACCACTTG 
TCTTACTTCC ATTTCGTGGG TCGGATCATG GGTCTGGCTG TGTTCCACGG ACACTACATC 
AATGGGGGTT TCACGGTGCC TTTCTACAAG CAGCTACTGG GGAAGCCCAT CCAGCTTTCT 
GATTTGGAAT CGGTGGACCC TGAACTCCAT AAGAGCCTAG TGTGGATTCT AGAGAATGAC 
ATCACCCCCG TGCTCGATCA CACGTTCTGC GTGGAGCACA ATGCTTTCGG GCGGATTCTT 
CAGCACGAAC TGAAACCCAA CGGCAGGAAC GTCCCCGTCA CAGAGGAGAA TAAGAAGGAA 
TATGTCCGGT TGTATGTAAA TTGGAGGTTC ATGAGGGGAA TCGAGGCCCA GTTCCTGGCT 
CTTCAGAAGG GGTTTAACGA GCTCATCCCT CAACACTTAT TGAAGCCTTT TGACCAGAAG 
GAGCTGGAGC TGATCATTGG CGGCCTAGAC AAGATCGACC TGGACGACTG GAAGTCCAAC 
ACGCGGCTGA AGCACTGTGG GGCCGACAGC AACGTGGTCC GGTGGTTCTG GCAGGCGGTA 
GAGACCTTCG ATGAGGAGCG GCGGGCCCGG CTGCTGCAGT TCGTGACAGG CTCCACGCGG 
GTCCCCCTCC AGGGCTTCAA GGCGCTGCAA GGCTCTACAG GCGCCGCAGG GCCCCGGCTC 
TTCACCATCC ACCTGATCGA CGCCAACACG GACAACCTGC CCAAGGCCCA CACCTGCTTT 
AACCGGATCG ACATCCCGCC CTACGAGTCG TACGAGAAGC TCTATGAGAA GCTGCTGACT 
GCGGTGGAGG AGACCTGTGG CTTTGCCGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225328.2
CDS	277..2472
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLESEGTVYEDSGPGRPLSCFMEEPAPYTDGPGAAAGGGTCRFVESPSQDQRLQAQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMTHQCQLKEPSQPPPPPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_005225271.4
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225271.4

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCCGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA CCTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGAGAC CTTAACAGCG TGAACTGCGA TGAACTTGGA 
CCACTGCCGC CCGGTTGGGA AGTCCGCAGC ACGGTTTCCG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCGCGGTTAC ACCACATCAT GACTCACCAG 
TGCCAACTGA AGGAGCCCAG CCAGCCACCA CCGCCACCCA GCGAGGGCTC CGTGGAGGAC 
GAGGAGCTGC CCGCCCAGAG GTACGAGAGA GATCTGGTCC AGAAACTGAA GGTCCTCAGA 
CACGAGCTCT CCTTGCAGCA GCCCCAGGCT GGCCACTGCC GCATCGAGGT GTCCAGAGAG 
GAGATATTTG AGGAGTCTTA TCGCCAGATA ATGAAGATGC GACCAAAAGA CTTGAAAAAG 
CGACTCATGG TGAAATTCCG AGGAGAAGAA GGCTTGGACT ATGGAGGGGT GGCGAGGGAG 
TGGCTTTATT TACTGTGCCA TGAAATGTTG AACCCGTACT ATGGACTCTT CCAGTACTCC 
ACGGACAACA TTTACATGTT GCAAATCAAC CCAGACTCTT CAATCAACCC TGACCACTTG 
TCTTACTTCC ATTTCGTGGG TCGGATCATG GGTCTGGCTG TGTTCCACGG ACACTACATC 
AATGGGGGTT TCACGGTGCC TTTCTACAAG CAGCTACTGG GGAAGCCCAT CCAGCTTTCT 
GATTTGGAAT CGGTGGACCC TGAACTCCAT AAGAGCCTAG TGTGGATTCT AGAGAATGAC 
ATCACCCCCG TGCTCGATCA CACGTTCTGC GTGGAGCACA ATGCTTTCGG GCGGATTCTT 
CAGCACGAAC TGAAACCCAA CGGCAGGAAC GTCCCCGTCA CAGAGGAGAA TAAGAAGGAA 
TATGTCCGGT TGTATGTAAA TTGGAGGTTC ATGAGGGGAA TCGAGGCCCA GTTCCTGGCT 
CTTCAGAAGG GGTTTAACGA GCTCATCCCT CAACACTTAT TGAAGCCTTT TGACCAGAAG 
GAGCTGGAGC TGATCATTGG CGGCCTAGAC AAGATCGACC TGGACGACTG GAAGTCCAAC 
ACGCGGCTGA AGCACTGTGG GGCCGACAGC AACGTGGTCC GGTGGTTCTG GCAGGCGGTA 
GAGACCTTCG ATGAGGAGCG GCGGGCCCGG CTGCTGCAGT TCGTGACAGG CTCCACGCGG 
GTCCCCCTCC AGGGCTTCAA GGCGCTGCAA GGCTCTACAG GCGCCGCAGG GCCCCGGCTC 
TTCACCATCC ACCTGATCGA CGCCAACACG GACAACCTGC CCAAGGCCCA CACCTGCTTT 
AACCGGATCG ACATCCCGCC CTACGAGTCG TACGAGAAGC TCTATGAGAA GCTGCTGACT 
GCGGTGGAGG AGACCTGTGG CTTTGCCGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_005225272.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225329.2 Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa230704
Clone ID Related Accession (Same CDS sequence)	XM_005225272.4
Accession Version	XM_005225272.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225272.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCCGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA CCTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGAGAC CTTAACAGCG TGAACTGCGA TGAACTTGGA 
CCACTGCCGC CCGGTTGGGA AGTCCGCAGC ACGGTTTCCG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCGCGGTTAC ACCACATCAT GACTCACCAG 
TGCCAACTGA AGGAGCCCAG CCAGCCACCA CCGCCACCCA GCGAGGGCTC CGTGGAGGAC 
GAGGAGCTGC CCGCCCAGAG GTACGAGAGA GATCTGGTCC AGAAACTGAA GGTCCTCAGA 
CACGAGCTCT CCTTGCAGCA GCCCCAGGCT GGCCACTGCC GCATCGAGGT GTCCAGAGAG 
GAGATATTTG AGGAGTCTTA TCGCCAGATA ATGAAGATGC GACCAAAAGA CTTGAAAAAG 
CGACTCATGG TGAAATTCCG AGGAGAAGAA GGCTTGGACT ATGGAGGGGT GGCGAGGGAG 
TGGCTTTATT TACTGTGCCA TGAAATGTTG AACCCGTACT ATGGACTCTT CCAGTACTCC 
ACGGACAACA TTTACATGTT GCAAATCAAC CCAGACTCTT CAATCAACCC TGACCACTTG 
TCTTACTTCC ATTTCGTGGG TCGGATCATG GGTCTGGCTG TGTTCCACGG ACACTACATC 
AATGGGGGTT TCACGGTGCC TTTCTACAAG CAGCTACTGG GGAAGCCCAT CCAGCTTTCT 
GATTTGGAAT CGGTGGACCC TGAACTCCAT AAGAGCCTAG TGTGGATTCT AGAGAATGAC 
ATCACCCCCG TGCTCGATCA CACGTTCTGC GTGGAGCACA ATGCTTTCGG GCGGATTCTT 
CAGCACGAAC TGAAACCCAA CGGCAGGAAC GTCCCCGTCA CAGAGGAGAA TAAGAAGGAA 
TATGTCCGGT TGTATGTAAA TTGGAGGTTC ATGAGGGGAA TCGAGGCCCA GTTCCTGGCT 
CTTCAGAAGG GGTTTAACGA GCTCATCCCT CAACACTTAT TGAAGCCTTT TGACCAGAAG 
GAGCTGGAGC TGATCATTGG CGGCCTAGAC AAGATCGACC TGGACGACTG GAAGTCCAAC 
ACGCGGCTGA AGCACTGTGG GGCCGACAGC AACGTGGTCC GGTGGTTCTG GCAGGCGGTA 
GAGACCTTCG ATGAGGAGCG GCGGGCCCGG CTGCTGCAGT TCGTGACAGG CTCCACGCGG 
GTCCCCCTCC AGGGCTTCAA GGCGCTGCAA GGCGCCGCAG GGCCCCGGCT CTTCACCATC 
CACCTGATCG ACGCCAACAC GGACAACCTG CCCAAGGCCC ACACCTGCTT TAACCGGATC 
GACATCCCGC CCTACGAGTC GTACGAGAAG CTCTATGAGA AGCTGCTGAC TGCGGTGGAG 
GAGACCTGTG GCTTTGCCGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225329.2
CDS	277..2463
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLESEGTVYEDSGPGRPLSCFMEEPAPYTDGPGAAAGGGTCRFVESPSQDQRLQAQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMTHQCQLKEPSQPPPPPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_005225272.4
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225272.4

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCCGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA CCTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGAGAC CTTAACAGCG TGAACTGCGA TGAACTTGGA 
CCACTGCCGC CCGGTTGGGA AGTCCGCAGC ACGGTTTCCG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCGCGGTTAC ACCACATCAT GACTCACCAG 
TGCCAACTGA AGGAGCCCAG CCAGCCACCA CCGCCACCCA GCGAGGGCTC CGTGGAGGAC 
GAGGAGCTGC CCGCCCAGAG GTACGAGAGA GATCTGGTCC AGAAACTGAA GGTCCTCAGA 
CACGAGCTCT CCTTGCAGCA GCCCCAGGCT GGCCACTGCC GCATCGAGGT GTCCAGAGAG 
GAGATATTTG AGGAGTCTTA TCGCCAGATA ATGAAGATGC GACCAAAAGA CTTGAAAAAG 
CGACTCATGG TGAAATTCCG AGGAGAAGAA GGCTTGGACT ATGGAGGGGT GGCGAGGGAG 
TGGCTTTATT TACTGTGCCA TGAAATGTTG AACCCGTACT ATGGACTCTT CCAGTACTCC 
ACGGACAACA TTTACATGTT GCAAATCAAC CCAGACTCTT CAATCAACCC TGACCACTTG 
TCTTACTTCC ATTTCGTGGG TCGGATCATG GGTCTGGCTG TGTTCCACGG ACACTACATC 
AATGGGGGTT TCACGGTGCC TTTCTACAAG CAGCTACTGG GGAAGCCCAT CCAGCTTTCT 
GATTTGGAAT CGGTGGACCC TGAACTCCAT AAGAGCCTAG TGTGGATTCT AGAGAATGAC 
ATCACCCCCG TGCTCGATCA CACGTTCTGC GTGGAGCACA ATGCTTTCGG GCGGATTCTT 
CAGCACGAAC TGAAACCCAA CGGCAGGAAC GTCCCCGTCA CAGAGGAGAA TAAGAAGGAA 
TATGTCCGGT TGTATGTAAA TTGGAGGTTC ATGAGGGGAA TCGAGGCCCA GTTCCTGGCT 
CTTCAGAAGG GGTTTAACGA GCTCATCCCT CAACACTTAT TGAAGCCTTT TGACCAGAAG 
GAGCTGGAGC TGATCATTGG CGGCCTAGAC AAGATCGACC TGGACGACTG GAAGTCCAAC 
ACGCGGCTGA AGCACTGTGG GGCCGACAGC AACGTGGTCC GGTGGTTCTG GCAGGCGGTA 
GAGACCTTCG ATGAGGAGCG GCGGGCCCGG CTGCTGCAGT TCGTGACAGG CTCCACGCGG 
GTCCCCCTCC AGGGCTTCAA GGCGCTGCAA GGCGCCGCAG GGCCCCGGCT CTTCACCATC 
CACCTGATCG ACGCCAACAC GGACAACCTG CCCAAGGCCC ACACCTGCTT TAACCGGATC 
GACATCCCGC CCTACGAGTC GTACGAGAAG CTCTATGAGA AGCTGCTGAC TGCGGTGGAG 
GAGACCTGTG GCTTTGCCGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_024985447.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841215.1 Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

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CloneID	OBa230705
Clone ID Related Accession (Same CDS sequence)	XM_024985447.1
Accession Version	XM_024985447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTTCA GTTTACAGAC ACGAGACAGG 
ATCGGCACTG GCGGCTCTGT GGTGGACTGC AGAGGCCTGC TGGAGAGCGA AGGAACCGTG 
TATGAAGACT CCGGGCCTGG AAGGCCCCTC AGCTGCTTCA TGGAGGAGCC AGCCCCGTAC 
ACGGATGGCC CCGGGGCCGC TGCTGGCGGG GGCACCTGCA GGTTTGTGGA ATCCCCGAGT 
CAAGACCAAA GACTTCAGGC CCAGCGACTG CGAAACCCTG AGGTGCGAGG GTCGCTCCAG 
ACGCCACAGA ACCGACCTCA CGGCCACCAG TCCCCGGAGC TGCCCGAAGG CTACGAGCAG 
AGAACCACGG TACAGGGCCA GGTGTACTTC CTGCACACGC AGACGGGAGT CAGCACGTGG 
CACGACCCCA GGATACCCAG AGACCTTAAC AGCGTGAACT GCGATGAACT TGGACCACTG 
CCGCCCGGTT GGGAAGTCCG CAGCACGGTT TCCGGGAGGA TATATTTTGT AGATCATAAT 
AACCGAACAA CCCAGTTTAC AGACCCGCGG TTACACCACA TCATGACTCA CCAGTGCCAA 
CTGAAGGAGC CCAGCCAGCC ACCACCGCCA CCCAGCGAGG GCTCCGTGGA GGACGAGGAG 
CTGCCCGCCC AGAGGTACGA GAGAGATCTG GTCCAGAAAC TGAAGGTCCT CAGACACGAG 
CTCTCCTTGC AGCAGCCCCA GGCTGGCCAC TGCCGCATCG AGGTGTCCAG AGAGGAGATA 
TTTGAGGAGT CTTATCGCCA GATAATGAAG ATGCGACCAA AAGACTTGAA AAAGCGACTC 
ATGGTGAAAT TCCGAGGAGA AGAAGGCTTG GACTATGGAG GGGTGGCGAG GGAGTGGCTT 
TATTTACTGT GCCATGAAAT GTTGAACCCG TACTATGGAC TCTTCCAGTA CTCCACGGAC 
AACATTTACA TGTTGCAAAT CAACCCAGAC TCTTCAATCA ACCCTGACCA CTTGTCTTAC 
TTCCATTTCG TGGGTCGGAT CATGGGTCTG GCTGTGTTCC ACGGACACTA CATCAATGGG 
GGTTTCACGG TGCCTTTCTA CAAGCAGCTA CTGGGGAAGC CCATCCAGCT TTCTGATTTG 
GAATCGGTGG ACCCTGAACT CCATAAGAGC CTAGTGTGGA TTCTAGAGAA TGACATCACC 
CCCGTGCTCG ATCACACGTT CTGCGTGGAG CACAATGCTT TCGGGCGGAT TCTTCAGCAC 
GAACTGAAAC CCAACGGCAG GAACGTCCCC GTCACAGAGG AGAATAAGAA GGAATATGTC 
CGGTTGTATG TAAATTGGAG GTTCATGAGG GGAATCGAGG CCCAGTTCCT GGCTCTTCAG 
AAGGGGTTTA ACGAGCTCAT CCCTCAACAC TTATTGAAGC CTTTTGACCA GAAGGAGCTG 
GAGCTGATCA TTGGCGGCCT AGACAAGATC GACCTGGACG ACTGGAAGTC CAACACGCGG 
CTGAAGCACT GTGGGGCCGA CAGCAACGTG GTCCGGTGGT TCTGGCAGGC GGTAGAGACC 
TTCGATGAGG AGCGGCGGGC CCGGCTGCTG CAGTTCGTGA CAGGCTCCAC GCGGGTCCCC 
CTCCAGGGCT TCAAGGCGCT GCAAGGCTCT ACAGGCGCCG CAGGGCCCCG GCTCTTCACC 
ATCCACCTGA TCGACGCCAA CACGGACAAC CTGCCCAAGG CCCACACCTG CTTTAACCGG 
ATCGACATCC CGCCCTACGA GTCGTACGAG AAGCTCTATG AGAAGCTGCT GACTGCGGTG 
GAGGAGACCT GTGGCTTTGC CGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841215.1
CDS	278..2407
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGFSLQTRDRIGTGGSVVDCRGLLESEGTVYEDSGPGRPLSCFMEEPAPYTDGPGAAAGGGTCRFVESPSQDQRLQAQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMTHQCQLKEPSQPPPPPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_024985447.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985447.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTTCA GTTTACAGAC ACGAGACAGG 
ATCGGCACTG GCGGCTCTGT GGTGGACTGC AGAGGCCTGC TGGAGAGCGA AGGAACCGTG 
TATGAAGACT CCGGGCCTGG AAGGCCCCTC AGCTGCTTCA TGGAGGAGCC AGCCCCGTAC 
ACGGATGGCC CCGGGGCCGC TGCTGGCGGG GGCACCTGCA GGTTTGTGGA ATCCCCGAGT 
CAAGACCAAA GACTTCAGGC CCAGCGACTG CGAAACCCTG AGGTGCGAGG GTCGCTCCAG 
ACGCCACAGA ACCGACCTCA CGGCCACCAG TCCCCGGAGC TGCCCGAAGG CTACGAGCAG 
AGAACCACGG TACAGGGCCA GGTGTACTTC CTGCACACGC AGACGGGAGT CAGCACGTGG 
CACGACCCCA GGATACCCAG AGACCTTAAC AGCGTGAACT GCGATGAACT TGGACCACTG 
CCGCCCGGTT GGGAAGTCCG CAGCACGGTT TCCGGGAGGA TATATTTTGT AGATCATAAT 
AACCGAACAA CCCAGTTTAC AGACCCGCGG TTACACCACA TCATGACTCA CCAGTGCCAA 
CTGAAGGAGC CCAGCCAGCC ACCACCGCCA CCCAGCGAGG GCTCCGTGGA GGACGAGGAG 
CTGCCCGCCC AGAGGTACGA GAGAGATCTG GTCCAGAAAC TGAAGGTCCT CAGACACGAG 
CTCTCCTTGC AGCAGCCCCA GGCTGGCCAC TGCCGCATCG AGGTGTCCAG AGAGGAGATA 
TTTGAGGAGT CTTATCGCCA GATAATGAAG ATGCGACCAA AAGACTTGAA AAAGCGACTC 
ATGGTGAAAT TCCGAGGAGA AGAAGGCTTG GACTATGGAG GGGTGGCGAG GGAGTGGCTT 
TATTTACTGT GCCATGAAAT GTTGAACCCG TACTATGGAC TCTTCCAGTA CTCCACGGAC 
AACATTTACA TGTTGCAAAT CAACCCAGAC TCTTCAATCA ACCCTGACCA CTTGTCTTAC 
TTCCATTTCG TGGGTCGGAT CATGGGTCTG GCTGTGTTCC ACGGACACTA CATCAATGGG 
GGTTTCACGG TGCCTTTCTA CAAGCAGCTA CTGGGGAAGC CCATCCAGCT TTCTGATTTG 
GAATCGGTGG ACCCTGAACT CCATAAGAGC CTAGTGTGGA TTCTAGAGAA TGACATCACC 
CCCGTGCTCG ATCACACGTT CTGCGTGGAG CACAATGCTT TCGGGCGGAT TCTTCAGCAC 
GAACTGAAAC CCAACGGCAG GAACGTCCCC GTCACAGAGG AGAATAAGAA GGAATATGTC 
CGGTTGTATG TAAATTGGAG GTTCATGAGG GGAATCGAGG CCCAGTTCCT GGCTCTTCAG 
AAGGGGTTTA ACGAGCTCAT CCCTCAACAC TTATTGAAGC CTTTTGACCA GAAGGAGCTG 
GAGCTGATCA TTGGCGGCCT AGACAAGATC GACCTGGACG ACTGGAAGTC CAACACGCGG 
CTGAAGCACT GTGGGGCCGA CAGCAACGTG GTCCGGTGGT TCTGGCAGGC GGTAGAGACC 
TTCGATGAGG AGCGGCGGGC CCGGCTGCTG CAGTTCGTGA CAGGCTCCAC GCGGGTCCCC 
CTCCAGGGCT TCAAGGCGCT GCAAGGCTCT ACAGGCGCCG CAGGGCCCCG GCTCTTCACC 
ATCCACCTGA TCGACGCCAA CACGGACAAC CTGCCCAAGG CCCACACCTG CTTTAACCGG 
ATCGACATCC CGCCCTACGA GTCGTACGAG AAGCTCTATG AGAAGCTGCT GACTGCGGTG 
GAGGAGACCT GTGGCTTTGC CGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_002698145.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002698191.4 Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.

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CloneID	OBa230706
Clone ID Related Accession (Same CDS sequence)	XM_002698145.6
Accession Version	XM_002698145.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002698145.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCCGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA CCTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGTCCC TGGGGGACCA CTCCTGGGGG AGATGGAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGCGTG 
AACTGCGATG AACTTGGACC ACTGCCGCCC GGTTGGGAAG TCCGCAGCAC GGTTTCCGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC GCGGTTACAC 
CACATCATGA CTCACCAGTG CCAACTGAAG GAGCCCAGCC AGCCACCACC GCCACCCAGC 
GAGGGCTCCG TGGAGGACGA GGAGCTGCCC GCCCAGAGGT ACGAGAGAGA TCTGGTCCAG 
AAACTGAAGG TCCTCAGACA CGAGCTCTCC TTGCAGCAGC CCCAGGCTGG CCACTGCCGC 
ATCGAGGTGT CCAGAGAGGA GATATTTGAG GAGTCTTATC GCCAGATAAT GAAGATGCGA 
CCAAAAGACT TGAAAAAGCG ACTCATGGTG AAATTCCGAG GAGAAGAAGG CTTGGACTAT 
GGAGGGGTGG CGAGGGAGTG GCTTTATTTA CTGTGCCATG AAATGTTGAA CCCGTACTAT 
GGACTCTTCC AGTACTCCAC GGACAACATT TACATGTTGC AAATCAACCC AGACTCTTCA 
ATCAACCCTG ACCACTTGTC TTACTTCCAT TTCGTGGGTC GGATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATCAA TGGGGGTTTC ACGGTGCCTT TCTACAAGCA GCTACTGGGG 
AAGCCCATCC AGCTTTCTGA TTTGGAATCG GTGGACCCTG AACTCCATAA GAGCCTAGTG 
TGGATTCTAG AGAATGACAT CACCCCCGTG CTCGATCACA CGTTCTGCGT GGAGCACAAT 
GCTTTCGGGC GGATTCTTCA GCACGAACTG AAACCCAACG GCAGGAACGT CCCCGTCACA 
GAGGAGAATA AGAAGGAATA TGTCCGGTTG TATGTAAATT GGAGGTTCAT GAGGGGAATC 
GAGGCCCAGT TCCTGGCTCT TCAGAAGGGG TTTAACGAGC TCATCCCTCA ACACTTATTG 
AAGCCTTTTG ACCAGAAGGA GCTGGAGCTG ATCATTGGCG GCCTAGACAA GATCGACCTG 
GACGACTGGA AGTCCAACAC GCGGCTGAAG CACTGTGGGG CCGACAGCAA CGTGGTCCGG 
TGGTTCTGGC AGGCGGTAGA GACCTTCGAT GAGGAGCGGC GGGCCCGGCT GCTGCAGTTC 
GTGACAGGCT CCACGCGGGT CCCCCTCCAG GGCTTCAAGG CGCTGCAAGG CTCTACAGGC 
GCCGCAGGGC CCCGGCTCTT CACCATCCAC CTGATCGACG CCAACACGGA CAACCTGCCC 
AAGGCCCACA CCTGCTTTAA CCGGATCGAC ATCCCGCCCT ACGAGTCGTA CGAGAAGCTC 
TATGAGAAGC TGCTGACTGC GGTGGAGGAG ACCTGTGGCT TTGCCGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002698191.4
CDS	280..2553
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLESEGTVYEDSGPGRPLSCFMEEPAPYTDGPGAAAGGGTCRFVESPSQDQRLQAQRLRNPEVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPWGTTPGGDGAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMTHQCQLKEPSQPPPPPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_002698145.6
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002698145.6

ORF Insert Sequence:

ATGTCGAACC CCGGGACGCG GAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTTGTGGACG GATCTGGGCA GTGCCACTCA ACGGACACGG TGAAGAACAC ACTGGACCCA 
AAGTGGAACC AGCACTACGA TCTATATGTT GGAAAAACGG ATTCTATAAC CATCAGCGTG 
TGGAACCACA AGAAGATTCA CAAGAAGCAG GGAGCCGGCT TCCTGGGGTG TGTGCGGCTG 
CTGTCCAACG CCATCAGCAG ATTAAAAGAT ACTGGCTACC AGCGTTTGGA TCTGTGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACACGA 
GACAGGATCG GCACTGGCGG CTCTGTGGTG GACTGCAGAG GCCTGCTGGA GAGCGAAGGA 
ACCGTGTATG AAGACTCCGG GCCTGGAAGG CCCCTCAGCT GCTTCATGGA GGAGCCAGCC 
CCGTACACGG ATGGCCCCGG GGCCGCTGCT GGCGGGGGCA CCTGCAGGTT TGTGGAATCC 
CCGAGTCAAG ACCAAAGACT TCAGGCCCAG CGACTGCGAA ACCCTGAGGT GCGAGGGTCG 
CTCCAGACGC CACAGAACCG ACCTCACGGC CACCAGTCCC CGGAGCTGCC CGAAGGCTAC 
GAGCAGAGAA CCACGGTACA GGGCCAGGTG TACTTCCTGC ACACGCAGAC GGGAGTCAGC 
ACGTGGCACG ACCCCAGGAT ACCCAGTCCC TGGGGGACCA CTCCTGGGGG AGATGGAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGCGTG 
AACTGCGATG AACTTGGACC ACTGCCGCCC GGTTGGGAAG TCCGCAGCAC GGTTTCCGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC GCGGTTACAC 
CACATCATGA CTCACCAGTG CCAACTGAAG GAGCCCAGCC AGCCACCACC GCCACCCAGC 
GAGGGCTCCG TGGAGGACGA GGAGCTGCCC GCCCAGAGGT ACGAGAGAGA TCTGGTCCAG 
AAACTGAAGG TCCTCAGACA CGAGCTCTCC TTGCAGCAGC CCCAGGCTGG CCACTGCCGC 
ATCGAGGTGT CCAGAGAGGA GATATTTGAG GAGTCTTATC GCCAGATAAT GAAGATGCGA 
CCAAAAGACT TGAAAAAGCG ACTCATGGTG AAATTCCGAG GAGAAGAAGG CTTGGACTAT 
GGAGGGGTGG CGAGGGAGTG GCTTTATTTA CTGTGCCATG AAATGTTGAA CCCGTACTAT 
GGACTCTTCC AGTACTCCAC GGACAACATT TACATGTTGC AAATCAACCC AGACTCTTCA 
ATCAACCCTG ACCACTTGTC TTACTTCCAT TTCGTGGGTC GGATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATCAA TGGGGGTTTC ACGGTGCCTT TCTACAAGCA GCTACTGGGG 
AAGCCCATCC AGCTTTCTGA TTTGGAATCG GTGGACCCTG AACTCCATAA GAGCCTAGTG 
TGGATTCTAG AGAATGACAT CACCCCCGTG CTCGATCACA CGTTCTGCGT GGAGCACAAT 
GCTTTCGGGC GGATTCTTCA GCACGAACTG AAACCCAACG GCAGGAACGT CCCCGTCACA 
GAGGAGAATA AGAAGGAATA TGTCCGGTTG TATGTAAATT GGAGGTTCAT GAGGGGAATC 
GAGGCCCAGT TCCTGGCTCT TCAGAAGGGG TTTAACGAGC TCATCCCTCA ACACTTATTG 
AAGCCTTTTG ACCAGAAGGA GCTGGAGCTG ATCATTGGCG GCCTAGACAA GATCGACCTG 
GACGACTGGA AGTCCAACAC GCGGCTGAAG CACTGTGGGG CCGACAGCAA CGTGGTCCGG 
TGGTTCTGGC AGGCGGTAGA GACCTTCGAT GAGGAGCGGC GGGCCCGGCT GCTGCAGTTC 
GTGACAGGCT CCACGCGGGT CCCCCTCCAG GGCTTCAAGG CGCTGCAAGG CTCTACAGGC 
GCCGCAGGGC CCCGGCTCTT CACCATCCAC CTGATCGACG CCAACACGGA CAACCTGCCC 
AAGGCCCACA CCTGCTTTAA CCGGATCGAC ATCCCGCCCT ACGAGTCGTA CGAGAAGCTC 
TATGAGAAGC TGCTGACTGC GGTGGAGGAG ACCTGTGGCT TTGCCGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SMURF1
Entrez Gene ID	513902
Full Name	SMAD specific E3 ubiquitin protein ligase 1
General protein information	Preferred Names SMAD specific E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase SMURF1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Gallus gallus (chicken)	SMURF1	XP_414794.3
Homo sapiens (human)	SMURF1	NP_065162.1
	SMURF1	XP_002803251.1
Canis lupus familiaris (dog)	SMURF1	XP_005621234.1
Xenopus tropicalis (tropical clawed frog)	LOC100491582	XP_004917897.1
Pan troglodytes (chimpanzee)	SMURF1	XP_528043.3
Bos taurus (cattle)	SMURF1	XP_002698191.3
Mus musculus (house mouse)	Smurf1	NP_001033716.1
Rattus norvegicus (Norway rat)	Smurf1	NP_001103068.1
Danio rerio (zebrafish)	smurf1	NP_001001943.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SMURF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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