Smurf1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006504884.1	XP_006504947.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_011241017.2	XP_011239319.1	E3 ubiquitin-protein ligase SMURF1 isoform X3
XM_006504884.2	XP_006504947.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_006504888.4	XP_006504951.1	E3 ubiquitin-protein ligase SMURF1 isoform X4
XM_011241016.2	XP_011239318.1	E3 ubiquitin-protein ligase SMURF1 isoform X2
NM_029438.3	NP_083714.3	E3 ubiquitin-protein ligase SMURF1 isoform 2
XM_011241016.1	XP_011239318.1	E3 ubiquitin-protein ligase SMURF1 isoform X2
XM_011241017.1	XP_011239319.1	E3 ubiquitin-protein ligase SMURF1 isoform X3
NM_001038627.1	NP_001033716.1	E3 ubiquitin-protein ligase SMURF1 isoform 1
XM_006504888.3	XP_006504951.1	E3 ubiquitin-protein ligase SMURF1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18629	NM_001038627.1 Latest version!	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$433.30 $619.00
OMu18749	NM_029438.3 Latest version!	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu36439	XM_006504884.2 Latest version!	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu36443	XM_006504888.4 Latest version!	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu71402	XM_011241016.2 Latest version!	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu71403	XM_011241017.2 Latest version!	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu36439	XM_006504884.1	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu71402	XM_011241016.1	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu71403	XM_011241017.1	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu36443	XM_006504888.3	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

Smurf1 ( XM_006504888.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504951.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu36443
Clone ID Related Accession (Same CDS sequence)	XM_006504888.4 , XM_006504888.3
Accession Version	XM_006504888.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504888.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC CTGCCCCATA TACAGATGGT ACTGGTGCAG CAGCAGGAGG CGGGAACTGC 
AGGTTTGTGG AGTCTCCAAG CCAAGATCAG AGACTCCTGG TACAGCGACT CCGAAATCCT 
GAGGTTCGAG GGCCCTTACA GACACCCCAG AACCGACCAC ATGGCCACCA GTCGCCAGAG 
CTGCCTGAAG GCTATGAGCA AAGGACAACA GTGCAGGGAC AAGTTTACTT TTTGCACACG 
CAGACTGGAG TCAGTACATG GCATGACCCC AGGATCCCCA GTCCCTTGGG GACCATTCCG 
GGGGGAGATG AAGCTTTTCT ATACGAATTC CTTCTACAAG GCCATACATC CGAGCCCAGA 
GACCTTAACA GTGTGAACTG CGATGAACTT GGGCCACTGC CTCCAGGCTG GGAAGTCCGA 
AGCACAGTGT CGGGAAGAAT CTATTTTGTA GATCACAATA ATAGGACAAC CCAGTTTACA 
GATCCACGGC TTCACCACAT CATGAATCAC CAGTGCCAAC TCAAGGAGCC CAGCCAGCCG 
CTGCAGCTGC CCAGTGAGGG CTCCGTGGAG GACGAGGAGC TTCCTGCCCA GAGATATGAG 
AGGGACTTAG TCCAGAAGCT CAAAGTTCTC AGGCACGAGC TCTCTCTTCA GCAGCCCCAG 
GCTGGTCACT GTCGAATAGA AGTCTCCAGA GAAGAGATAT TTGAGGAGTC GTATCGCCAG 
ATCATGAAGA TGCGGCCAAA AGACCTAAAG AAGCGCCTGA TGGTGAAGTT CCGAGGGGAG 
GAAGGTTTGG ACTATGGTGG AGTGGCTCGG GAGTGGCTGT ATTTGTTGTG CCATGAAATG 
TTGAACCCGT ACTATGGACT CTTCCAGTAT TCCACGGACA ATATTTACAC ACTGCAGATC 
AACCCAGATT CTTCTATCAA CCCTGACCAT CTGTCATACT TCCACTTTGT GGGTCGCATC 
ATGGGTCTGG CTGTGTTCCA CGGACACTAC ATAAATGGGG GTTTCACAGT TCCGTTCTAC 
AAGCAGCTCC TGGGGAAGCC AATCCAGCTG TCGGACCTGG AGTCCGTGGA CCCAGAACTG 
CATAAGAGCT TGGTGTGGAT TCTAGAGAAT GACATCACGC CTGTGTTGGA TCATACCTTC 
TGCGTGGAGC ACAACGCTTT CGGGCGGATT CTCCAGCATG AACTGAAACC CAATGGCAGA 
AATGTGCCTG TCACTGAGGA GAACAAGAAG GAATACGTCC GGCTGTATGT GAACTGGAGG 
TTTATGAGAG GAATCGAAGC CCAGTTCTTA GCACTTCAGA AGGGGTTTAA CGAACTCATT 
CCCCAACACT TGCTGAAGCC CTTTGACCAG AAGGAACTAG AGCTGATAAT AGGTGGGCTG 
GATAAGATAG ACCTGAACGA CTGGAAGTCC AACACCCGGC TGAAACACTG TGTGGCAGAC 
AGCAACATCG TCAGGTGGTT CTGGCAGGCG GTGGAGACCT TCGATGAGGA GAGGAGAGCC 
AGACTCCTGC AGTTTGTGAC AGGATCCACA AGAGTTCCTC TCCAAGGCTT CAAGGCTCTG 
CAAGGCTCTA CAGGCGCGGC AGGGCCCCGG CTGTTCACCA TTCACCTGAT AGACGCCAAT 
ACAGACAACC TGCCCAAGGC CCATACCTGC TTTAATCGGA TCGACATCCC ACCCTATGAG 
TCCTATGAGA AGCTCTATGA GAAGCTGCTG ACAGCGGTGG AGGAAACCTG TGGCTTTGCA 
GTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504951.1
CDS	540..2288
Translation	MEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPLGTIPGGDEAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_006504888.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504888.3

ORF Insert Sequence:

ATGGAGGAAC CTGCCCCATA TACAGATGGT ACTGGTGCAG CAGCAGGAGG CGGGAACTGC 
AGGTTTGTGG AGTCTCCAAG CCAAGATCAG AGACTCCTGG TACAGCGACT CCGAAATCCT 
GAGGTTCGAG GGCCCTTACA GACACCCCAG AACCGACCAC ATGGCCACCA GTCGCCAGAG 
CTGCCTGAAG GCTATGAGCA AAGGACAACA GTGCAGGGAC AAGTTTACTT TTTGCACACG 
CAGACTGGAG TCAGTACATG GCATGACCCC AGGATCCCCA GTCCCTTGGG GACCATTCCG 
GGGGGAGATG AAGCTTTTCT ATACGAATTC CTTCTACAAG GCCATACATC CGAGCCCAGA 
GACCTTAACA GTGTGAACTG CGATGAACTT GGGCCACTGC CTCCAGGCTG GGAAGTCCGA 
AGCACAGTGT CGGGAAGAAT CTATTTTGTA GATCACAATA ATAGGACAAC CCAGTTTACA 
GATCCACGGC TTCACCACAT CATGAATCAC CAGTGCCAAC TCAAGGAGCC CAGCCAGCCG 
CTGCAGCTGC CCAGTGAGGG CTCCGTGGAG GACGAGGAGC TTCCTGCCCA GAGATATGAG 
AGGGACTTAG TCCAGAAGCT CAAAGTTCTC AGGCACGAGC TCTCTCTTCA GCAGCCCCAG 
GCTGGTCACT GTCGAATAGA AGTCTCCAGA GAAGAGATAT TTGAGGAGTC GTATCGCCAG 
ATCATGAAGA TGCGGCCAAA AGACCTAAAG AAGCGCCTGA TGGTGAAGTT CCGAGGGGAG 
GAAGGTTTGG ACTATGGTGG AGTGGCTCGG GAGTGGCTGT ATTTGTTGTG CCATGAAATG 
TTGAACCCGT ACTATGGACT CTTCCAGTAT TCCACGGACA ATATTTACAC ACTGCAGATC 
AACCCAGATT CTTCTATCAA CCCTGACCAT CTGTCATACT TCCACTTTGT GGGTCGCATC 
ATGGGTCTGG CTGTGTTCCA CGGACACTAC ATAAATGGGG GTTTCACAGT TCCGTTCTAC 
AAGCAGCTCC TGGGGAAGCC AATCCAGCTG TCGGACCTGG AGTCCGTGGA CCCAGAACTG 
CATAAGAGCT TGGTGTGGAT TCTAGAGAAT GACATCACGC CTGTGTTGGA TCATACCTTC 
TGCGTGGAGC ACAACGCTTT CGGGCGGATT CTCCAGCATG AACTGAAACC CAATGGCAGA 
AATGTGCCTG TCACTGAGGA GAACAAGAAG GAATACGTCC GGCTGTATGT GAACTGGAGG 
TTTATGAGAG GAATCGAAGC CCAGTTCTTA GCACTTCAGA AGGGGTTTAA CGAACTCATT 
CCCCAACACT TGCTGAAGCC CTTTGACCAG AAGGAACTAG AGCTGATAAT AGGTGGGCTG 
GATAAGATAG ACCTGAACGA CTGGAAGTCC AACACCCGGC TGAAACACTG TGTGGCAGAC 
AGCAACATCG TCAGGTGGTT CTGGCAGGCG GTGGAGACCT TCGATGAGGA GAGGAGAGCC 
AGACTCCTGC AGTTTGTGAC AGGATCCACA AGAGTTCCTC TCCAAGGCTT CAAGGCTCTG 
CAAGGCTCTA CAGGCGCGGC AGGGCCCCGG CTGTTCACCA TTCACCTGAT AGACGCCAAT 
ACAGACAACC TGCCCAAGGC CCATACCTGC TTTAATCGGA TCGACATCCC ACCCTATGAG 
TCCTATGAGA AGCTCTATGA GAAGCTGCTG ACAGCGGTGG AGGAAACCTG TGGCTTTGCA 
GTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( NM_001038627.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001033716.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18629
Clone ID Related Accession (Same CDS sequence)	NM_001038627.1
Accession Version	NM_001038627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113295.5, CX568994.1, CB524571.1, AK015264.1 and AK034736.1. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single C57BL/6J strain transcript was available for the full length of the gene. The extent of the sequence in this record is supported by orthologous human data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK154491.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGAGAC CTTAACAGTG TGAACTGCGA TGAACTTGGG 
CCACTGCCTC CAGGCTGGGA AGTCCGAAGC ACAGTGTCGG GAAGAATCTA TTTTGTAGAT 
CACAATAATA GGACAACCCA GTTTACAGAT CCACGGCTTC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CAGCTGCCCA GTGAGGGCTC CGTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATATGAGAGG GACTTAGTCC AGAAGCTCAA AGTTCTCAGG 
CACGAGCTCT CTCTTCAGCA GCCCCAGGCT GGTCACTGTC GAATAGAAGT CTCCAGAGAA 
GAGATATTTG AGGAGTCGTA TCGCCAGATC ATGAAGATGC GGCCAAAAGA CCTAAAGAAG 
CGCCTGATGG TGAAGTTCCG AGGGGAGGAA GGTTTGGACT ATGGTGGAGT GGCTCGGGAG 
TGGCTGTATT TGTTGTGCCA TGAAATGTTG AACCCGTACT ATGGACTCTT CCAGTATTCC 
ACGGACAATA TTTACACACT GCAGATCAAC CCAGATTCTT CTATCAACCC TGACCATCTG 
TCATACTTCC ACTTTGTGGG TCGCATCATG GGTCTGGCTG TGTTCCACGG ACACTACATA 
AATGGGGGTT TCACAGTTCC GTTCTACAAG CAGCTCCTGG GGAAGCCAAT CCAGCTGTCG 
GACCTGGAGT CCGTGGACCC AGAACTGCAT AAGAGCTTGG TGTGGATTCT AGAGAATGAC 
ATCACGCCTG TGTTGGATCA TACCTTCTGC GTGGAGCACA ACGCTTTCGG GCGGATTCTC 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCTGTCA CTGAGGAGAA CAAGAAGGAA 
TACGTCCGGC TGTATGTGAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCA 
CTTCAGAAGG GGTTTAACGA ACTCATTCCC CAACACTTGC TGAAGCCCTT TGACCAGAAG 
GAACTAGAGC TGATAATAGG TGGGCTGGAT AAGATAGACC TGAACGACTG GAAGTCCAAC 
ACCCGGCTGA AACACTGTGT GGCAGACAGC AACATCGTCA GGTGGTTCTG GCAGGCGGTG 
GAGACCTTCG ATGAGGAGAG GAGAGCCAGA CTCCTGCAGT TTGTGACAGG ATCCACAAGA 
GTTCCTCTCC AAGGCTTCAA GGCTCTGCAA GGCTCTACAG GCGCGGCAGG GCCCCGGCTG 
TTCACCATTC ACCTGATAGA CGCCAATACA GACAACCTGC CCAAGGCCCA TACCTGCTTT 
AATCGGATCG ACATCCCACC CTATGAGTCC TATGAGAAGC TCTATGAGAA GCTGCTGACA 
GCGGTGGAGG AAACCTGTGG CTTTGCAGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033716.1
CDS	276..2471
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	NM_001038627.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038627.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGAGAC CTTAACAGTG TGAACTGCGA TGAACTTGGG 
CCACTGCCTC CAGGCTGGGA AGTCCGAAGC ACAGTGTCGG GAAGAATCTA TTTTGTAGAT 
CACAATAATA GGACAACCCA GTTTACAGAT CCACGGCTTC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CAGCTGCCCA GTGAGGGCTC CGTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATATGAGAGG GACTTAGTCC AGAAGCTCAA AGTTCTCAGG 
CACGAGCTCT CTCTTCAGCA GCCCCAGGCT GGTCACTGTC GAATAGAAGT CTCCAGAGAA 
GAGATATTTG AGGAGTCGTA TCGCCAGATC ATGAAGATGC GGCCAAAAGA CCTAAAGAAG 
CGCCTGATGG TGAAGTTCCG AGGGGAGGAA GGTTTGGACT ATGGTGGAGT GGCTCGGGAG 
TGGCTGTATT TGTTGTGCCA TGAAATGTTG AACCCGTACT ATGGACTCTT CCAGTATTCC 
ACGGACAATA TTTACACACT GCAGATCAAC CCAGATTCTT CTATCAACCC TGACCATCTG 
TCATACTTCC ACTTTGTGGG TCGCATCATG GGTCTGGCTG TGTTCCACGG ACACTACATA 
AATGGGGGTT TCACAGTTCC GTTCTACAAG CAGCTCCTGG GGAAGCCAAT CCAGCTGTCG 
GACCTGGAGT CCGTGGACCC AGAACTGCAT AAGAGCTTGG TGTGGATTCT AGAGAATGAC 
ATCACGCCTG TGTTGGATCA TACCTTCTGC GTGGAGCACA ACGCTTTCGG GCGGATTCTC 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCTGTCA CTGAGGAGAA CAAGAAGGAA 
TACGTCCGGC TGTATGTGAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCA 
CTTCAGAAGG GGTTTAACGA ACTCATTCCC CAACACTTGC TGAAGCCCTT TGACCAGAAG 
GAACTAGAGC TGATAATAGG TGGGCTGGAT AAGATAGACC TGAACGACTG GAAGTCCAAC 
ACCCGGCTGA AACACTGTGT GGCAGACAGC AACATCGTCA GGTGGTTCTG GCAGGCGGTG 
GAGACCTTCG ATGAGGAGAG GAGAGCCAGA CTCCTGCAGT TTGTGACAGG ATCCACAAGA 
GTTCCTCTCC AAGGCTTCAA GGCTCTGCAA GGCTCTACAG GCGCGGCAGG GCCCCGGCTG 
TTCACCATTC ACCTGATAGA CGCCAATACA GACAACCTGC CCAAGGCCCA TACCTGCTTT 
AATCGGATCG ACATCCCACC CTATGAGTCC TATGAGAAGC TCTATGAGAA GCTGCTGACA 
GCGGTGGAGG AAACCTGTGG CTTTGCAGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( NM_029438.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083714.3 Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18749
Clone ID Related Accession (Same CDS sequence)	NM_029438.3
Accession Version	NM_029438.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113295.5, CX568994.1, CB524571.1 and AK015264.1. On Feb 8, 2006 this sequence version replaced NM_029438.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single C57BL/6J strain transcript was available for the full length of the gene. The extent of the sequence in this record is supported by orthologous human data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC029097.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGAGAC CTTAACAGTG TGAACTGCGA TGAACTTGGG 
CCACTGCCTC CAGGCTGGGA AGTCCGAAGC ACAGTGTCGG GAAGAATCTA TTTTGTAGAT 
CACAATAATA GGACAACCCA GTTTACAGAT CCACGGCTTC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CAGCTGCCCA GTGAGGGCTC CGTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATATGAGAGG GACTTAGTCC AGAAGCTCAA AGTTCTCAGG 
CACGAGCTCT CTCTTCAGCA GCCCCAGGCT GGTCACTGTC GAATAGAAGT CTCCAGAGAA 
GAGATATTTG AGGAGTCGTA TCGCCAGATC ATGAAGATGC GGCCAAAAGA CCTAAAGAAG 
CGCCTGATGG TGAAGTTCCG AGGGGAGGAA GGTTTGGACT ATGGTGGAGT GGCTCGGGAG 
TGGCTGTATT TGTTGTGCCA TGAAATGTTG AACCCGTACT ATGGACTCTT CCAGTATTCC 
ACGGACAATA TTTACACACT GCAGATCAAC CCAGATTCTT CTATCAACCC TGACCATCTG 
TCATACTTCC ACTTTGTGGG TCGCATCATG GGTCTGGCTG TGTTCCACGG ACACTACATA 
AATGGGGGTT TCACAGTTCC GTTCTACAAG CAGCTCCTGG GGAAGCCAAT CCAGCTGTCG 
GACCTGGAGT CCGTGGACCC AGAACTGCAT AAGAGCTTGG TGTGGATTCT AGAGAATGAC 
ATCACGCCTG TGTTGGATCA TACCTTCTGC GTGGAGCACA ACGCTTTCGG GCGGATTCTC 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCTGTCA CTGAGGAGAA CAAGAAGGAA 
TACGTCCGGC TGTATGTGAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCA 
CTTCAGAAGG GGTTTAACGA ACTCATTCCC CAACACTTGC TGAAGCCCTT TGACCAGAAG 
GAACTAGAGC TGATAATAGG TGGGCTGGAT AAGATAGACC TGAACGACTG GAAGTCCAAC 
ACCCGGCTGA AACACTGTGT GGCAGACAGC AACATCGTCA GGTGGTTCTG GCAGGCGGTG 
GAGACCTTCG ATGAGGAGAG GAGAGCCAGA CTCCTGCAGT TTGTGACAGG ATCCACAAGA 
GTTCCTCTCC AAGGCTTCAA GGCTCTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATT 
CACCTGATAG ACGCCAATAC AGACAACCTG CCCAAGGCCC ATACCTGCTT TAATCGGATC 
GACATCCCAC CCTATGAGTC CTATGAGAAG CTCTATGAGA AGCTGCTGAC AGCGGTGGAG 
GAAACCTGTG GCTTTGCAGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083714.3
CDS	276..2462
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	NM_029438.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029438.3

ORF Insert Sequence:

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGAGAC CTTAACAGTG TGAACTGCGA TGAACTTGGG 
CCACTGCCTC CAGGCTGGGA AGTCCGAAGC ACAGTGTCGG GAAGAATCTA TTTTGTAGAT 
CACAATAATA GGACAACCCA GTTTACAGAT CCACGGCTTC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CAGCTGCCCA GTGAGGGCTC CGTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATATGAGAGG GACTTAGTCC AGAAGCTCAA AGTTCTCAGG 
CACGAGCTCT CTCTTCAGCA GCCCCAGGCT GGTCACTGTC GAATAGAAGT CTCCAGAGAA 
GAGATATTTG AGGAGTCGTA TCGCCAGATC ATGAAGATGC GGCCAAAAGA CCTAAAGAAG 
CGCCTGATGG TGAAGTTCCG AGGGGAGGAA GGTTTGGACT ATGGTGGAGT GGCTCGGGAG 
TGGCTGTATT TGTTGTGCCA TGAAATGTTG AACCCGTACT ATGGACTCTT CCAGTATTCC 
ACGGACAATA TTTACACACT GCAGATCAAC CCAGATTCTT CTATCAACCC TGACCATCTG 
TCATACTTCC ACTTTGTGGG TCGCATCATG GGTCTGGCTG TGTTCCACGG ACACTACATA 
AATGGGGGTT TCACAGTTCC GTTCTACAAG CAGCTCCTGG GGAAGCCAAT CCAGCTGTCG 
GACCTGGAGT CCGTGGACCC AGAACTGCAT AAGAGCTTGG TGTGGATTCT AGAGAATGAC 
ATCACGCCTG TGTTGGATCA TACCTTCTGC GTGGAGCACA ACGCTTTCGG GCGGATTCTC 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCTGTCA CTGAGGAGAA CAAGAAGGAA 
TACGTCCGGC TGTATGTGAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCA 
CTTCAGAAGG GGTTTAACGA ACTCATTCCC CAACACTTGC TGAAGCCCTT TGACCAGAAG 
GAACTAGAGC TGATAATAGG TGGGCTGGAT AAGATAGACC TGAACGACTG GAAGTCCAAC 
ACCCGGCTGA AACACTGTGT GGCAGACAGC AACATCGTCA GGTGGTTCTG GCAGGCGGTG 
GAGACCTTCG ATGAGGAGAG GAGAGCCAGA CTCCTGCAGT TTGTGACAGG ATCCACAAGA 
GTTCCTCTCC AAGGCTTCAA GGCTCTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATT 
CACCTGATAG ACGCCAATAC AGACAACCTG CCCAAGGCCC ATACCTGCTT TAATCGGATC 
GACATCCCAC CCTATGAGTC CTATGAGAAG CTCTATGAGA AGCTGCTGAC AGCGGTGGAG 
GAAACCTGTG GCTTTGCAGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_006504884.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504947.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.

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CloneID	OMu36439
Clone ID Related Accession (Same CDS sequence)	XM_006504884.1 , XM_006504884.2
Accession Version	XM_006504884.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGTCCC TTGGGGACCA TTCCGGGGGG AGATGAAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCCGAGC CCAGAGACCT TAACAGTGTG 
AACTGCGATG AACTTGGGCC ACTGCCTCCA GGCTGGGAAG TCCGAAGCAC AGTGTCGGGA 
AGAATCTATT TTGTAGATCA CAATAATAGG ACAACCCAGT TTACAGATCC ACGGCTTCAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCA GCTGCCCAGT 
GAGGGCTCCG TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ATGAGAGGGA CTTAGTCCAG 
AAGCTCAAAG TTCTCAGGCA CGAGCTCTCT CTTCAGCAGC CCCAGGCTGG TCACTGTCGA 
ATAGAAGTCT CCAGAGAAGA GATATTTGAG GAGTCGTATC GCCAGATCAT GAAGATGCGG 
CCAAAAGACC TAAAGAAGCG CCTGATGGTG AAGTTCCGAG GGGAGGAAGG TTTGGACTAT 
GGTGGAGTGG CTCGGGAGTG GCTGTATTTG TTGTGCCATG AAATGTTGAA CCCGTACTAT 
GGACTCTTCC AGTATTCCAC GGACAATATT TACACACTGC AGATCAACCC AGATTCTTCT 
ATCAACCCTG ACCATCTGTC ATACTTCCAC TTTGTGGGTC GCATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATAAA TGGGGGTTTC ACAGTTCCGT TCTACAAGCA GCTCCTGGGG 
AAGCCAATCC AGCTGTCGGA CCTGGAGTCC GTGGACCCAG AACTGCATAA GAGCTTGGTG 
TGGATTCTAG AGAATGACAT CACGCCTGTG TTGGATCATA CCTTCTGCGT GGAGCACAAC 
GCTTTCGGGC GGATTCTCCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCTGTCACT 
GAGGAGAACA AGAAGGAATA CGTCCGGCTG TATGTGAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCACT TCAGAAGGGG TTTAACGAAC TCATTCCCCA ACACTTGCTG 
AAGCCCTTTG ACCAGAAGGA ACTAGAGCTG ATAATAGGTG GGCTGGATAA GATAGACCTG 
AACGACTGGA AGTCCAACAC CCGGCTGAAA CACTGTGTGG CAGACAGCAA CATCGTCAGG 
TGGTTCTGGC AGGCGGTGGA GACCTTCGAT GAGGAGAGGA GAGCCAGACT CCTGCAGTTT 
GTGACAGGAT CCACAAGAGT TCCTCTCCAA GGCTTCAAGG CTCTGCAAGG CTCTACAGGC 
GCGGCAGGGC CCCGGCTGTT CACCATTCAC CTGATAGACG CCAATACAGA CAACCTGCCC 
AAGGCCCATA CCTGCTTTAA TCGGATCGAC ATCCCACCCT ATGAGTCCTA TGAGAAGCTC 
TATGAGAAGC TGCTGACAGC GGTGGAGGAA ACCTGTGGCT TTGCAGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504947.1
CDS	271..2544
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPLGTIPGGDEAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_006504884.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504884.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGTCCC TTGGGGACCA TTCCGGGGGG AGATGAAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCCGAGC CCAGAGACCT TAACAGTGTG 
AACTGCGATG AACTTGGGCC ACTGCCTCCA GGCTGGGAAG TCCGAAGCAC AGTGTCGGGA 
AGAATCTATT TTGTAGATCA CAATAATAGG ACAACCCAGT TTACAGATCC ACGGCTTCAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCA GCTGCCCAGT 
GAGGGCTCCG TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ATGAGAGGGA CTTAGTCCAG 
AAGCTCAAAG TTCTCAGGCA CGAGCTCTCT CTTCAGCAGC CCCAGGCTGG TCACTGTCGA 
ATAGAAGTCT CCAGAGAAGA GATATTTGAG GAGTCGTATC GCCAGATCAT GAAGATGCGG 
CCAAAAGACC TAAAGAAGCG CCTGATGGTG AAGTTCCGAG GGGAGGAAGG TTTGGACTAT 
GGTGGAGTGG CTCGGGAGTG GCTGTATTTG TTGTGCCATG AAATGTTGAA CCCGTACTAT 
GGACTCTTCC AGTATTCCAC GGACAATATT TACACACTGC AGATCAACCC AGATTCTTCT 
ATCAACCCTG ACCATCTGTC ATACTTCCAC TTTGTGGGTC GCATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATAAA TGGGGGTTTC ACAGTTCCGT TCTACAAGCA GCTCCTGGGG 
AAGCCAATCC AGCTGTCGGA CCTGGAGTCC GTGGACCCAG AACTGCATAA GAGCTTGGTG 
TGGATTCTAG AGAATGACAT CACGCCTGTG TTGGATCATA CCTTCTGCGT GGAGCACAAC 
GCTTTCGGGC GGATTCTCCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCTGTCACT 
GAGGAGAACA AGAAGGAATA CGTCCGGCTG TATGTGAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCACT TCAGAAGGGG TTTAACGAAC TCATTCCCCA ACACTTGCTG 
AAGCCCTTTG ACCAGAAGGA ACTAGAGCTG ATAATAGGTG GGCTGGATAA GATAGACCTG 
AACGACTGGA AGTCCAACAC CCGGCTGAAA CACTGTGTGG CAGACAGCAA CATCGTCAGG 
TGGTTCTGGC AGGCGGTGGA GACCTTCGAT GAGGAGAGGA GAGCCAGACT CCTGCAGTTT 
GTGACAGGAT CCACAAGAGT TCCTCTCCAA GGCTTCAAGG CTCTGCAAGG CTCTACAGGC 
GCGGCAGGGC CCCGGCTGTT CACCATTCAC CTGATAGACG CCAATACAGA CAACCTGCCC 
AAGGCCCATA CCTGCTTTAA TCGGATCGAC ATCCCACCCT ATGAGTCCTA TGAGAAGCTC 
TATGAGAAGC TGCTGACAGC GGTGGAGGAA ACCTGTGGCT TTGCAGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_011241016.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239318.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu71402
Clone ID Related Accession (Same CDS sequence)	XM_011241016.2 , XM_011241016.1
Accession Version	XM_011241016.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGTCCC TTGGGGACCA TTCCGGGGGG AGATGAAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCCGAGC CCAGAGACCT TAACAGTGTG 
AACTGCGATG AACTTGGGCC ACTGCCTCCA GGCTGGGAAG TCCGAAGCAC AGTGTCGGGA 
AGAATCTATT TTGTAGATCA CAATAATAGG ACAACCCAGT TTACAGATCC ACGGCTTCAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCA GCTGCCCAGT 
GAGGGCTCCG TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ATGAGAGGGA CTTAGTCCAG 
AAGCTCAAAG TTCTCAGGCA CGAGCTCTCT CTTCAGCAGC CCCAGGCTGG TCACTGTCGA 
ATAGAAGTCT CCAGAGAAGA GATATTTGAG GAGTCGTATC GCCAGATCAT GAAGATGCGG 
CCAAAAGACC TAAAGAAGCG CCTGATGGTG AAGTTCCGAG GGGAGGAAGG TTTGGACTAT 
GGTGGAGTGG CTCGGGAGTG GCTGTATTTG TTGTGCCATG AAATGTTGAA CCCGTACTAT 
GGACTCTTCC AGTATTCCAC GGACAATATT TACACACTGC AGATCAACCC AGATTCTTCT 
ATCAACCCTG ACCATCTGTC ATACTTCCAC TTTGTGGGTC GCATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATAAA TGGGGGTTTC ACAGTTCCGT TCTACAAGCA GCTCCTGGGG 
AAGCCAATCC AGCTGTCGGA CCTGGAGTCC GTGGACCCAG AACTGCATAA GAGCTTGGTG 
TGGATTCTAG AGAATGACAT CACGCCTGTG TTGGATCATA CCTTCTGCGT GGAGCACAAC 
GCTTTCGGGC GGATTCTCCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCTGTCACT 
GAGGAGAACA AGAAGGAATA CGTCCGGCTG TATGTGAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCACT TCAGAAGGGG TTTAACGAAC TCATTCCCCA ACACTTGCTG 
AAGCCCTTTG ACCAGAAGGA ACTAGAGCTG ATAATAGGTG GGCTGGATAA GATAGACCTG 
AACGACTGGA AGTCCAACAC CCGGCTGAAA CACTGTGTGG CAGACAGCAA CATCGTCAGG 
TGGTTCTGGC AGGCGGTGGA GACCTTCGAT GAGGAGAGGA GAGCCAGACT CCTGCAGTTT 
GTGACAGGAT CCACAAGAGT TCCTCTCCAA GGCTTCAAGG CTCTGCAAGG CGCGGCAGGG 
CCCCGGCTGT TCACCATTCA CCTGATAGAC GCCAATACAG ACAACCTGCC CAAGGCCCAT 
ACCTGCTTTA ATCGGATCGA CATCCCACCC TATGAGTCCT ATGAGAAGCT CTATGAGAAG 
CTGCTGACAG CGGTGGAGGA AACCTGTGGC TTTGCAGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239318.1
CDS	271..2535
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPLGTIPGGDEAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_011241016.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241016.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGTCCC TTGGGGACCA TTCCGGGGGG AGATGAAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCCGAGC CCAGAGACCT TAACAGTGTG 
AACTGCGATG AACTTGGGCC ACTGCCTCCA GGCTGGGAAG TCCGAAGCAC AGTGTCGGGA 
AGAATCTATT TTGTAGATCA CAATAATAGG ACAACCCAGT TTACAGATCC ACGGCTTCAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCA GCTGCCCAGT 
GAGGGCTCCG TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ATGAGAGGGA CTTAGTCCAG 
AAGCTCAAAG TTCTCAGGCA CGAGCTCTCT CTTCAGCAGC CCCAGGCTGG TCACTGTCGA 
ATAGAAGTCT CCAGAGAAGA GATATTTGAG GAGTCGTATC GCCAGATCAT GAAGATGCGG 
CCAAAAGACC TAAAGAAGCG CCTGATGGTG AAGTTCCGAG GGGAGGAAGG TTTGGACTAT 
GGTGGAGTGG CTCGGGAGTG GCTGTATTTG TTGTGCCATG AAATGTTGAA CCCGTACTAT 
GGACTCTTCC AGTATTCCAC GGACAATATT TACACACTGC AGATCAACCC AGATTCTTCT 
ATCAACCCTG ACCATCTGTC ATACTTCCAC TTTGTGGGTC GCATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATAAA TGGGGGTTTC ACAGTTCCGT TCTACAAGCA GCTCCTGGGG 
AAGCCAATCC AGCTGTCGGA CCTGGAGTCC GTGGACCCAG AACTGCATAA GAGCTTGGTG 
TGGATTCTAG AGAATGACAT CACGCCTGTG TTGGATCATA CCTTCTGCGT GGAGCACAAC 
GCTTTCGGGC GGATTCTCCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCTGTCACT 
GAGGAGAACA AGAAGGAATA CGTCCGGCTG TATGTGAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCACT TCAGAAGGGG TTTAACGAAC TCATTCCCCA ACACTTGCTG 
AAGCCCTTTG ACCAGAAGGA ACTAGAGCTG ATAATAGGTG GGCTGGATAA GATAGACCTG 
AACGACTGGA AGTCCAACAC CCGGCTGAAA CACTGTGTGG CAGACAGCAA CATCGTCAGG 
TGGTTCTGGC AGGCGGTGGA GACCTTCGAT GAGGAGAGGA GAGCCAGACT CCTGCAGTTT 
GTGACAGGAT CCACAAGAGT TCCTCTCCAA GGCTTCAAGG CTCTGCAAGG CGCGGCAGGG 
CCCCGGCTGT TCACCATTCA CCTGATAGAC GCCAATACAG ACAACCTGCC CAAGGCCCAT 
ACCTGCTTTA ATCGGATCGA CATCCCACCC TATGAGTCCT ATGAGAAGCT CTATGAGAAG 
CTGCTGACAG CGGTGGAGGA AACCTGTGGC TTTGCAGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_011241017.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239319.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X3, mRNA.

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CloneID	OMu71403
Clone ID Related Accession (Same CDS sequence)	XM_011241017.2 , XM_011241017.1
Accession Version	XM_011241017.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGTCAC CAGTGCCAAC TCAAGGAGCC CAGCCAGCCG 
CTGCAGCTGC CCAGTGAGGG CTCCGTGGAG GACGAGGAGC TTCCTGCCCA GAGATATGAG 
AGGGACTTAG TCCAGAAGCT CAAAGTTCTC AGGCACGAGC TCTCTCTTCA GCAGCCCCAG 
GCTGGTCACT GTCGAATAGA AGTCTCCAGA GAAGAGATAT TTGAGGAGTC GTATCGCCAG 
ATCATGAAGA TGCGGCCAAA AGACCTAAAG AAGCGCCTGA TGGTGAAGTT CCGAGGGGAG 
GAAGGTTTGG ACTATGGTGG AGTGGCTCGG GAGTGGCTGT ATTTGTTGTG CCATGAAATG 
TTGAACCCGT ACTATGGACT CTTCCAGTAT TCCACGGACA ATATTTACAC ACTGCAGATC 
AACCCAGATT CTTCTATCAA CCCTGACCAT CTGTCATACT TCCACTTTGT GGGTCGCATC 
ATGGGTCTGG CTGTGTTCCA CGGACACTAC ATAAATGGGG GTTTCACAGT TCCGTTCTAC 
AAGCAGCTCC TGGGGAAGCC AATCCAGCTG TCGGACCTGG AGTCCGTGGA CCCAGAACTG 
CATAAGAGCT TGGTGTGGAT TCTAGAGAAT GACATCACGC CTGTGTTGGA TCATACCTTC 
TGCGTGGAGC ACAACGCTTT CGGGCGGATT CTCCAGCATG AACTGAAACC CAATGGCAGA 
AATGTGCCTG TCACTGAGGA GAACAAGAAG GAATACGTCC GGCTGTATGT GAACTGGAGG 
TTTATGAGAG GAATCGAAGC CCAGTTCTTA GCACTTCAGA AGGGGTTTAA CGAACTCATT 
CCCCAACACT TGCTGAAGCC CTTTGACCAG AAGGAACTAG AGCTGATAAT AGGTGGGCTG 
GATAAGATAG ACCTGAACGA CTGGAAGTCC AACACCCGGC TGAAACACTG TGTGGCAGAC 
AGCAACATCG TCAGGTGGTT CTGGCAGGCG GTGGAGACCT TCGATGAGGA GAGGAGAGCC 
AGACTCCTGC AGTTTGTGAC AGGATCCACA AGAGTTCCTC TCCAAGGCTT CAAGGCTCTG 
CAAGGCTCTA CAGGCGCGGC AGGGCCCCGG CTGTTCACCA TTCACCTGAT AGACGCCAAT 
ACAGACAACC TGCCCAAGGC CCATACCTGC TTTAATCGGA TCGACATCCC ACCCTATGAG 
TCCTATGAGA AGCTCTATGA GAAGCTGCTG ACAGCGGTGG AGGAAACCTG TGGCTTTGCA 
GTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239319.1
CDS	271..2319
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_011241017.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241017.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGTCAC CAGTGCCAAC TCAAGGAGCC CAGCCAGCCG 
CTGCAGCTGC CCAGTGAGGG CTCCGTGGAG GACGAGGAGC TTCCTGCCCA GAGATATGAG 
AGGGACTTAG TCCAGAAGCT CAAAGTTCTC AGGCACGAGC TCTCTCTTCA GCAGCCCCAG 
GCTGGTCACT GTCGAATAGA AGTCTCCAGA GAAGAGATAT TTGAGGAGTC GTATCGCCAG 
ATCATGAAGA TGCGGCCAAA AGACCTAAAG AAGCGCCTGA TGGTGAAGTT CCGAGGGGAG 
GAAGGTTTGG ACTATGGTGG AGTGGCTCGG GAGTGGCTGT ATTTGTTGTG CCATGAAATG 
TTGAACCCGT ACTATGGACT CTTCCAGTAT TCCACGGACA ATATTTACAC ACTGCAGATC 
AACCCAGATT CTTCTATCAA CCCTGACCAT CTGTCATACT TCCACTTTGT GGGTCGCATC 
ATGGGTCTGG CTGTGTTCCA CGGACACTAC ATAAATGGGG GTTTCACAGT TCCGTTCTAC 
AAGCAGCTCC TGGGGAAGCC AATCCAGCTG TCGGACCTGG AGTCCGTGGA CCCAGAACTG 
CATAAGAGCT TGGTGTGGAT TCTAGAGAAT GACATCACGC CTGTGTTGGA TCATACCTTC 
TGCGTGGAGC ACAACGCTTT CGGGCGGATT CTCCAGCATG AACTGAAACC CAATGGCAGA 
AATGTGCCTG TCACTGAGGA GAACAAGAAG GAATACGTCC GGCTGTATGT GAACTGGAGG 
TTTATGAGAG GAATCGAAGC CCAGTTCTTA GCACTTCAGA AGGGGTTTAA CGAACTCATT 
CCCCAACACT TGCTGAAGCC CTTTGACCAG AAGGAACTAG AGCTGATAAT AGGTGGGCTG 
GATAAGATAG ACCTGAACGA CTGGAAGTCC AACACCCGGC TGAAACACTG TGTGGCAGAC 
AGCAACATCG TCAGGTGGTT CTGGCAGGCG GTGGAGACCT TCGATGAGGA GAGGAGAGCC 
AGACTCCTGC AGTTTGTGAC AGGATCCACA AGAGTTCCTC TCCAAGGCTT CAAGGCTCTG 
CAAGGCTCTA CAGGCGCGGC AGGGCCCCGG CTGTTCACCA TTCACCTGAT AGACGCCAAT 
ACAGACAACC TGCCCAAGGC CCATACCTGC TTTAATCGGA TCGACATCCC ACCCTATGAG 
TCCTATGAGA AGCTCTATGA GAAGCTGCTG ACAGCGGTGG AGGAAACCTG TGGCTTTGCA 
GTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_006504884.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504947.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.

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CloneID	OMu36439
Clone ID Related Accession (Same CDS sequence)	XM_006504884.1 , XM_006504884.2
Accession Version	XM_006504884.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGTCCC TTGGGGACCA TTCCGGGGGG AGATGAAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCCGAGC CCAGAGACCT TAACAGTGTG 
AACTGCGATG AACTTGGGCC ACTGCCTCCA GGCTGGGAAG TCCGAAGCAC AGTGTCGGGA 
AGAATCTATT TTGTAGATCA CAATAATAGG ACAACCCAGT TTACAGATCC ACGGCTTCAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCA GCTGCCCAGT 
GAGGGCTCCG TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ATGAGAGGGA CTTAGTCCAG 
AAGCTCAAAG TTCTCAGGCA CGAGCTCTCT CTTCAGCAGC CCCAGGCTGG TCACTGTCGA 
ATAGAAGTCT CCAGAGAAGA GATATTTGAG GAGTCGTATC GCCAGATCAT GAAGATGCGG 
CCAAAAGACC TAAAGAAGCG CCTGATGGTG AAGTTCCGAG GGGAGGAAGG TTTGGACTAT 
GGTGGAGTGG CTCGGGAGTG GCTGTATTTG TTGTGCCATG AAATGTTGAA CCCGTACTAT 
GGACTCTTCC AGTATTCCAC GGACAATATT TACACACTGC AGATCAACCC AGATTCTTCT 
ATCAACCCTG ACCATCTGTC ATACTTCCAC TTTGTGGGTC GCATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATAAA TGGGGGTTTC ACAGTTCCGT TCTACAAGCA GCTCCTGGGG 
AAGCCAATCC AGCTGTCGGA CCTGGAGTCC GTGGACCCAG AACTGCATAA GAGCTTGGTG 
TGGATTCTAG AGAATGACAT CACGCCTGTG TTGGATCATA CCTTCTGCGT GGAGCACAAC 
GCTTTCGGGC GGATTCTCCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCTGTCACT 
GAGGAGAACA AGAAGGAATA CGTCCGGCTG TATGTGAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCACT TCAGAAGGGG TTTAACGAAC TCATTCCCCA ACACTTGCTG 
AAGCCCTTTG ACCAGAAGGA ACTAGAGCTG ATAATAGGTG GGCTGGATAA GATAGACCTG 
AACGACTGGA AGTCCAACAC CCGGCTGAAA CACTGTGTGG CAGACAGCAA CATCGTCAGG 
TGGTTCTGGC AGGCGGTGGA GACCTTCGAT GAGGAGAGGA GAGCCAGACT CCTGCAGTTT 
GTGACAGGAT CCACAAGAGT TCCTCTCCAA GGCTTCAAGG CTCTGCAAGG CTCTACAGGC 
GCGGCAGGGC CCCGGCTGTT CACCATTCAC CTGATAGACG CCAATACAGA CAACCTGCCC 
AAGGCCCATA CCTGCTTTAA TCGGATCGAC ATCCCACCCT ATGAGTCCTA TGAGAAGCTC 
TATGAGAAGC TGCTGACAGC GGTGGAGGAA ACCTGTGGCT TTGCAGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504947.1
CDS	345..2618
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPLGTIPGGDEAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_006504884.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504884.2

ORF Insert Sequence:

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGTCCC TTGGGGACCA TTCCGGGGGG AGATGAAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCCGAGC CCAGAGACCT TAACAGTGTG 
AACTGCGATG AACTTGGGCC ACTGCCTCCA GGCTGGGAAG TCCGAAGCAC AGTGTCGGGA 
AGAATCTATT TTGTAGATCA CAATAATAGG ACAACCCAGT TTACAGATCC ACGGCTTCAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCA GCTGCCCAGT 
GAGGGCTCCG TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ATGAGAGGGA CTTAGTCCAG 
AAGCTCAAAG TTCTCAGGCA CGAGCTCTCT CTTCAGCAGC CCCAGGCTGG TCACTGTCGA 
ATAGAAGTCT CCAGAGAAGA GATATTTGAG GAGTCGTATC GCCAGATCAT GAAGATGCGG 
CCAAAAGACC TAAAGAAGCG CCTGATGGTG AAGTTCCGAG GGGAGGAAGG TTTGGACTAT 
GGTGGAGTGG CTCGGGAGTG GCTGTATTTG TTGTGCCATG AAATGTTGAA CCCGTACTAT 
GGACTCTTCC AGTATTCCAC GGACAATATT TACACACTGC AGATCAACCC AGATTCTTCT 
ATCAACCCTG ACCATCTGTC ATACTTCCAC TTTGTGGGTC GCATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATAAA TGGGGGTTTC ACAGTTCCGT TCTACAAGCA GCTCCTGGGG 
AAGCCAATCC AGCTGTCGGA CCTGGAGTCC GTGGACCCAG AACTGCATAA GAGCTTGGTG 
TGGATTCTAG AGAATGACAT CACGCCTGTG TTGGATCATA CCTTCTGCGT GGAGCACAAC 
GCTTTCGGGC GGATTCTCCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCTGTCACT 
GAGGAGAACA AGAAGGAATA CGTCCGGCTG TATGTGAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCACT TCAGAAGGGG TTTAACGAAC TCATTCCCCA ACACTTGCTG 
AAGCCCTTTG ACCAGAAGGA ACTAGAGCTG ATAATAGGTG GGCTGGATAA GATAGACCTG 
AACGACTGGA AGTCCAACAC CCGGCTGAAA CACTGTGTGG CAGACAGCAA CATCGTCAGG 
TGGTTCTGGC AGGCGGTGGA GACCTTCGAT GAGGAGAGGA GAGCCAGACT CCTGCAGTTT 
GTGACAGGAT CCACAAGAGT TCCTCTCCAA GGCTTCAAGG CTCTGCAAGG CTCTACAGGC 
GCGGCAGGGC CCCGGCTGTT CACCATTCAC CTGATAGACG CCAATACAGA CAACCTGCCC 
AAGGCCCATA CCTGCTTTAA TCGGATCGAC ATCCCACCCT ATGAGTCCTA TGAGAAGCTC 
TATGAGAAGC TGCTGACAGC GGTGGAGGAA ACCTGTGGCT TTGCAGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_006504888.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504951.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X4, mRNA.

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CloneID	OMu36443
Clone ID Related Accession (Same CDS sequence)	XM_006504888.4 , XM_006504888.3
Accession Version	XM_006504888.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504888.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC CTGCCCCATA TACAGATGGT ACTGGTGCAG CAGCAGGAGG CGGGAACTGC 
AGGTTTGTGG AGTCTCCAAG CCAAGATCAG AGACTCCTGG TACAGCGACT CCGAAATCCT 
GAGGTTCGAG GGCCCTTACA GACACCCCAG AACCGACCAC ATGGCCACCA GTCGCCAGAG 
CTGCCTGAAG GCTATGAGCA AAGGACAACA GTGCAGGGAC AAGTTTACTT TTTGCACACG 
CAGACTGGAG TCAGTACATG GCATGACCCC AGGATCCCCA GTCCCTTGGG GACCATTCCG 
GGGGGAGATG AAGCTTTTCT ATACGAATTC CTTCTACAAG GCCATACATC CGAGCCCAGA 
GACCTTAACA GTGTGAACTG CGATGAACTT GGGCCACTGC CTCCAGGCTG GGAAGTCCGA 
AGCACAGTGT CGGGAAGAAT CTATTTTGTA GATCACAATA ATAGGACAAC CCAGTTTACA 
GATCCACGGC TTCACCACAT CATGAATCAC CAGTGCCAAC TCAAGGAGCC CAGCCAGCCG 
CTGCAGCTGC CCAGTGAGGG CTCCGTGGAG GACGAGGAGC TTCCTGCCCA GAGATATGAG 
AGGGACTTAG TCCAGAAGCT CAAAGTTCTC AGGCACGAGC TCTCTCTTCA GCAGCCCCAG 
GCTGGTCACT GTCGAATAGA AGTCTCCAGA GAAGAGATAT TTGAGGAGTC GTATCGCCAG 
ATCATGAAGA TGCGGCCAAA AGACCTAAAG AAGCGCCTGA TGGTGAAGTT CCGAGGGGAG 
GAAGGTTTGG ACTATGGTGG AGTGGCTCGG GAGTGGCTGT ATTTGTTGTG CCATGAAATG 
TTGAACCCGT ACTATGGACT CTTCCAGTAT TCCACGGACA ATATTTACAC ACTGCAGATC 
AACCCAGATT CTTCTATCAA CCCTGACCAT CTGTCATACT TCCACTTTGT GGGTCGCATC 
ATGGGTCTGG CTGTGTTCCA CGGACACTAC ATAAATGGGG GTTTCACAGT TCCGTTCTAC 
AAGCAGCTCC TGGGGAAGCC AATCCAGCTG TCGGACCTGG AGTCCGTGGA CCCAGAACTG 
CATAAGAGCT TGGTGTGGAT TCTAGAGAAT GACATCACGC CTGTGTTGGA TCATACCTTC 
TGCGTGGAGC ACAACGCTTT CGGGCGGATT CTCCAGCATG AACTGAAACC CAATGGCAGA 
AATGTGCCTG TCACTGAGGA GAACAAGAAG GAATACGTCC GGCTGTATGT GAACTGGAGG 
TTTATGAGAG GAATCGAAGC CCAGTTCTTA GCACTTCAGA AGGGGTTTAA CGAACTCATT 
CCCCAACACT TGCTGAAGCC CTTTGACCAG AAGGAACTAG AGCTGATAAT AGGTGGGCTG 
GATAAGATAG ACCTGAACGA CTGGAAGTCC AACACCCGGC TGAAACACTG TGTGGCAGAC 
AGCAACATCG TCAGGTGGTT CTGGCAGGCG GTGGAGACCT TCGATGAGGA GAGGAGAGCC 
AGACTCCTGC AGTTTGTGAC AGGATCCACA AGAGTTCCTC TCCAAGGCTT CAAGGCTCTG 
CAAGGCTCTA CAGGCGCGGC AGGGCCCCGG CTGTTCACCA TTCACCTGAT AGACGCCAAT 
ACAGACAACC TGCCCAAGGC CCATACCTGC TTTAATCGGA TCGACATCCC ACCCTATGAG 
TCCTATGAGA AGCTCTATGA GAAGCTGCTG ACAGCGGTGG AGGAAACCTG TGGCTTTGCA 
GTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504951.1
CDS	538..2286
Translation	MEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPLGTIPGGDEAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_006504888.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504888.4

ORF Insert Sequence:

ATGGAGGAAC CTGCCCCATA TACAGATGGT ACTGGTGCAG CAGCAGGAGG CGGGAACTGC 
AGGTTTGTGG AGTCTCCAAG CCAAGATCAG AGACTCCTGG TACAGCGACT CCGAAATCCT 
GAGGTTCGAG GGCCCTTACA GACACCCCAG AACCGACCAC ATGGCCACCA GTCGCCAGAG 
CTGCCTGAAG GCTATGAGCA AAGGACAACA GTGCAGGGAC AAGTTTACTT TTTGCACACG 
CAGACTGGAG TCAGTACATG GCATGACCCC AGGATCCCCA GTCCCTTGGG GACCATTCCG 
GGGGGAGATG AAGCTTTTCT ATACGAATTC CTTCTACAAG GCCATACATC CGAGCCCAGA 
GACCTTAACA GTGTGAACTG CGATGAACTT GGGCCACTGC CTCCAGGCTG GGAAGTCCGA 
AGCACAGTGT CGGGAAGAAT CTATTTTGTA GATCACAATA ATAGGACAAC CCAGTTTACA 
GATCCACGGC TTCACCACAT CATGAATCAC CAGTGCCAAC TCAAGGAGCC CAGCCAGCCG 
CTGCAGCTGC CCAGTGAGGG CTCCGTGGAG GACGAGGAGC TTCCTGCCCA GAGATATGAG 
AGGGACTTAG TCCAGAAGCT CAAAGTTCTC AGGCACGAGC TCTCTCTTCA GCAGCCCCAG 
GCTGGTCACT GTCGAATAGA AGTCTCCAGA GAAGAGATAT TTGAGGAGTC GTATCGCCAG 
ATCATGAAGA TGCGGCCAAA AGACCTAAAG AAGCGCCTGA TGGTGAAGTT CCGAGGGGAG 
GAAGGTTTGG ACTATGGTGG AGTGGCTCGG GAGTGGCTGT ATTTGTTGTG CCATGAAATG 
TTGAACCCGT ACTATGGACT CTTCCAGTAT TCCACGGACA ATATTTACAC ACTGCAGATC 
AACCCAGATT CTTCTATCAA CCCTGACCAT CTGTCATACT TCCACTTTGT GGGTCGCATC 
ATGGGTCTGG CTGTGTTCCA CGGACACTAC ATAAATGGGG GTTTCACAGT TCCGTTCTAC 
AAGCAGCTCC TGGGGAAGCC AATCCAGCTG TCGGACCTGG AGTCCGTGGA CCCAGAACTG 
CATAAGAGCT TGGTGTGGAT TCTAGAGAAT GACATCACGC CTGTGTTGGA TCATACCTTC 
TGCGTGGAGC ACAACGCTTT CGGGCGGATT CTCCAGCATG AACTGAAACC CAATGGCAGA 
AATGTGCCTG TCACTGAGGA GAACAAGAAG GAATACGTCC GGCTGTATGT GAACTGGAGG 
TTTATGAGAG GAATCGAAGC CCAGTTCTTA GCACTTCAGA AGGGGTTTAA CGAACTCATT 
CCCCAACACT TGCTGAAGCC CTTTGACCAG AAGGAACTAG AGCTGATAAT AGGTGGGCTG 
GATAAGATAG ACCTGAACGA CTGGAAGTCC AACACCCGGC TGAAACACTG TGTGGCAGAC 
AGCAACATCG TCAGGTGGTT CTGGCAGGCG GTGGAGACCT TCGATGAGGA GAGGAGAGCC 
AGACTCCTGC AGTTTGTGAC AGGATCCACA AGAGTTCCTC TCCAAGGCTT CAAGGCTCTG 
CAAGGCTCTA CAGGCGCGGC AGGGCCCCGG CTGTTCACCA TTCACCTGAT AGACGCCAAT 
ACAGACAACC TGCCCAAGGC CCATACCTGC TTTAATCGGA TCGACATCCC ACCCTATGAG 
TCCTATGAGA AGCTCTATGA GAAGCTGCTG ACAGCGGTGG AGGAAACCTG TGGCTTTGCA 
GTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_011241016.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239318.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.

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CloneID	OMu71402
Clone ID Related Accession (Same CDS sequence)	XM_011241016.2 , XM_011241016.1
Accession Version	XM_011241016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGTCCC TTGGGGACCA TTCCGGGGGG AGATGAAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCCGAGC CCAGAGACCT TAACAGTGTG 
AACTGCGATG AACTTGGGCC ACTGCCTCCA GGCTGGGAAG TCCGAAGCAC AGTGTCGGGA 
AGAATCTATT TTGTAGATCA CAATAATAGG ACAACCCAGT TTACAGATCC ACGGCTTCAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCA GCTGCCCAGT 
GAGGGCTCCG TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ATGAGAGGGA CTTAGTCCAG 
AAGCTCAAAG TTCTCAGGCA CGAGCTCTCT CTTCAGCAGC CCCAGGCTGG TCACTGTCGA 
ATAGAAGTCT CCAGAGAAGA GATATTTGAG GAGTCGTATC GCCAGATCAT GAAGATGCGG 
CCAAAAGACC TAAAGAAGCG CCTGATGGTG AAGTTCCGAG GGGAGGAAGG TTTGGACTAT 
GGTGGAGTGG CTCGGGAGTG GCTGTATTTG TTGTGCCATG AAATGTTGAA CCCGTACTAT 
GGACTCTTCC AGTATTCCAC GGACAATATT TACACACTGC AGATCAACCC AGATTCTTCT 
ATCAACCCTG ACCATCTGTC ATACTTCCAC TTTGTGGGTC GCATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATAAA TGGGGGTTTC ACAGTTCCGT TCTACAAGCA GCTCCTGGGG 
AAGCCAATCC AGCTGTCGGA CCTGGAGTCC GTGGACCCAG AACTGCATAA GAGCTTGGTG 
TGGATTCTAG AGAATGACAT CACGCCTGTG TTGGATCATA CCTTCTGCGT GGAGCACAAC 
GCTTTCGGGC GGATTCTCCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCTGTCACT 
GAGGAGAACA AGAAGGAATA CGTCCGGCTG TATGTGAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCACT TCAGAAGGGG TTTAACGAAC TCATTCCCCA ACACTTGCTG 
AAGCCCTTTG ACCAGAAGGA ACTAGAGCTG ATAATAGGTG GGCTGGATAA GATAGACCTG 
AACGACTGGA AGTCCAACAC CCGGCTGAAA CACTGTGTGG CAGACAGCAA CATCGTCAGG 
TGGTTCTGGC AGGCGGTGGA GACCTTCGAT GAGGAGAGGA GAGCCAGACT CCTGCAGTTT 
GTGACAGGAT CCACAAGAGT TCCTCTCCAA GGCTTCAAGG CTCTGCAAGG CGCGGCAGGG 
CCCCGGCTGT TCACCATTCA CCTGATAGAC GCCAATACAG ACAACCTGCC CAAGGCCCAT 
ACCTGCTTTA ATCGGATCGA CATCCCACCC TATGAGTCCT ATGAGAAGCT CTATGAGAAG 
CTGCTGACAG CGGTGGAGGA AACCTGTGGC TTTGCAGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239318.1
CDS	345..2609
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPLGTIPGGDEAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_011241016.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241016.2

ORF Insert Sequence:

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGTCCC TTGGGGACCA TTCCGGGGGG AGATGAAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCCGAGC CCAGAGACCT TAACAGTGTG 
AACTGCGATG AACTTGGGCC ACTGCCTCCA GGCTGGGAAG TCCGAAGCAC AGTGTCGGGA 
AGAATCTATT TTGTAGATCA CAATAATAGG ACAACCCAGT TTACAGATCC ACGGCTTCAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCA GCTGCCCAGT 
GAGGGCTCCG TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ATGAGAGGGA CTTAGTCCAG 
AAGCTCAAAG TTCTCAGGCA CGAGCTCTCT CTTCAGCAGC CCCAGGCTGG TCACTGTCGA 
ATAGAAGTCT CCAGAGAAGA GATATTTGAG GAGTCGTATC GCCAGATCAT GAAGATGCGG 
CCAAAAGACC TAAAGAAGCG CCTGATGGTG AAGTTCCGAG GGGAGGAAGG TTTGGACTAT 
GGTGGAGTGG CTCGGGAGTG GCTGTATTTG TTGTGCCATG AAATGTTGAA CCCGTACTAT 
GGACTCTTCC AGTATTCCAC GGACAATATT TACACACTGC AGATCAACCC AGATTCTTCT 
ATCAACCCTG ACCATCTGTC ATACTTCCAC TTTGTGGGTC GCATCATGGG TCTGGCTGTG 
TTCCACGGAC ACTACATAAA TGGGGGTTTC ACAGTTCCGT TCTACAAGCA GCTCCTGGGG 
AAGCCAATCC AGCTGTCGGA CCTGGAGTCC GTGGACCCAG AACTGCATAA GAGCTTGGTG 
TGGATTCTAG AGAATGACAT CACGCCTGTG TTGGATCATA CCTTCTGCGT GGAGCACAAC 
GCTTTCGGGC GGATTCTCCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCTGTCACT 
GAGGAGAACA AGAAGGAATA CGTCCGGCTG TATGTGAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCACT TCAGAAGGGG TTTAACGAAC TCATTCCCCA ACACTTGCTG 
AAGCCCTTTG ACCAGAAGGA ACTAGAGCTG ATAATAGGTG GGCTGGATAA GATAGACCTG 
AACGACTGGA AGTCCAACAC CCGGCTGAAA CACTGTGTGG CAGACAGCAA CATCGTCAGG 
TGGTTCTGGC AGGCGGTGGA GACCTTCGAT GAGGAGAGGA GAGCCAGACT CCTGCAGTTT 
GTGACAGGAT CCACAAGAGT TCCTCTCCAA GGCTTCAAGG CTCTGCAAGG CGCGGCAGGG 
CCCCGGCTGT TCACCATTCA CCTGATAGAC GCCAATACAG ACAACCTGCC CAAGGCCCAT 
ACCTGCTTTA ATCGGATCGA CATCCCACCC TATGAGTCCT ATGAGAAGCT CTATGAGAAG 
CTGCTGACAG CGGTGGAGGA AACCTGTGGC TTTGCAGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf1 ( XM_011241017.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239319.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu71403
Clone ID Related Accession (Same CDS sequence)	XM_011241017.2 , XM_011241017.1
Accession Version	XM_011241017.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGTCAC CAGTGCCAAC TCAAGGAGCC CAGCCAGCCG 
CTGCAGCTGC CCAGTGAGGG CTCCGTGGAG GACGAGGAGC TTCCTGCCCA GAGATATGAG 
AGGGACTTAG TCCAGAAGCT CAAAGTTCTC AGGCACGAGC TCTCTCTTCA GCAGCCCCAG 
GCTGGTCACT GTCGAATAGA AGTCTCCAGA GAAGAGATAT TTGAGGAGTC GTATCGCCAG 
ATCATGAAGA TGCGGCCAAA AGACCTAAAG AAGCGCCTGA TGGTGAAGTT CCGAGGGGAG 
GAAGGTTTGG ACTATGGTGG AGTGGCTCGG GAGTGGCTGT ATTTGTTGTG CCATGAAATG 
TTGAACCCGT ACTATGGACT CTTCCAGTAT TCCACGGACA ATATTTACAC ACTGCAGATC 
AACCCAGATT CTTCTATCAA CCCTGACCAT CTGTCATACT TCCACTTTGT GGGTCGCATC 
ATGGGTCTGG CTGTGTTCCA CGGACACTAC ATAAATGGGG GTTTCACAGT TCCGTTCTAC 
AAGCAGCTCC TGGGGAAGCC AATCCAGCTG TCGGACCTGG AGTCCGTGGA CCCAGAACTG 
CATAAGAGCT TGGTGTGGAT TCTAGAGAAT GACATCACGC CTGTGTTGGA TCATACCTTC 
TGCGTGGAGC ACAACGCTTT CGGGCGGATT CTCCAGCATG AACTGAAACC CAATGGCAGA 
AATGTGCCTG TCACTGAGGA GAACAAGAAG GAATACGTCC GGCTGTATGT GAACTGGAGG 
TTTATGAGAG GAATCGAAGC CCAGTTCTTA GCACTTCAGA AGGGGTTTAA CGAACTCATT 
CCCCAACACT TGCTGAAGCC CTTTGACCAG AAGGAACTAG AGCTGATAAT AGGTGGGCTG 
GATAAGATAG ACCTGAACGA CTGGAAGTCC AACACCCGGC TGAAACACTG TGTGGCAGAC 
AGCAACATCG TCAGGTGGTT CTGGCAGGCG GTGGAGACCT TCGATGAGGA GAGGAGAGCC 
AGACTCCTGC AGTTTGTGAC AGGATCCACA AGAGTTCCTC TCCAAGGCTT CAAGGCTCTG 
CAAGGCTCTA CAGGCGCGGC AGGGCCCCGG CTGTTCACCA TTCACCTGAT AGACGCCAAT 
ACAGACAACC TGCCCAAGGC CCATACCTGC TTTAATCGGA TCGACATCCC ACCCTATGAG 
TCCTATGAGA AGCTCTATGA GAAGCTGCTG ACAGCGGTGG AGGAAACCTG TGGCTTTGCA 
GTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239319.1
CDS	345..2393
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSHQCQLKEPSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_011241017.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241017.2

ORF Insert Sequence:

ATGTCGAACC CCGGGACCCG TAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTATTG 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGACTCC CCGACCCCTT TGCCAAGATT 
GTTGTGGACG GCTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC CCTGGACCCA 
AAGTGGAACC AGCACTATGA CCTGTATGTT GGGAAAACGG ACTCGATAAC CATCAGTGTG 
TGGAACCACA AGAAGATCCA CAAGAAGCAG GGGGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTGAAAGAC ACTGGCTACC AGCGTTTGGA TCTATGCAAA 
CTAAATCCCT CAGATACTGA TGCAGTTCGT GGCCAAATAG TGGTCAGTTT ACAGACCCGA 
GACAGAATAG GCGGTGGAGG GTCAGTGGTG GACTGCAGAG GGCTGCTGGA GAACGAAGGA 
ACAGTGTATG AAGACTCAGG CCCTGGAAGG CCGCTCAGCT GCCTCATGGA GGAACCTGCC 
CCATATACAG ATGGTACTGG TGCAGCAGCA GGAGGCGGGA ACTGCAGGTT TGTGGAGTCT 
CCAAGCCAAG ATCAGAGACT CCTGGTACAG CGACTCCGAA ATCCTGAGGT TCGAGGGCCC 
TTACAGACAC CCCAGAACCG ACCACATGGC CACCAGTCGC CAGAGCTGCC TGAAGGCTAT 
GAGCAAAGGA CAACAGTGCA GGGACAAGTT TACTTTTTGC ACACGCAGAC TGGAGTCAGT 
ACATGGCATG ACCCCAGGAT CCCCAGTCAC CAGTGCCAAC TCAAGGAGCC CAGCCAGCCG 
CTGCAGCTGC CCAGTGAGGG CTCCGTGGAG GACGAGGAGC TTCCTGCCCA GAGATATGAG 
AGGGACTTAG TCCAGAAGCT CAAAGTTCTC AGGCACGAGC TCTCTCTTCA GCAGCCCCAG 
GCTGGTCACT GTCGAATAGA AGTCTCCAGA GAAGAGATAT TTGAGGAGTC GTATCGCCAG 
ATCATGAAGA TGCGGCCAAA AGACCTAAAG AAGCGCCTGA TGGTGAAGTT CCGAGGGGAG 
GAAGGTTTGG ACTATGGTGG AGTGGCTCGG GAGTGGCTGT ATTTGTTGTG CCATGAAATG 
TTGAACCCGT ACTATGGACT CTTCCAGTAT TCCACGGACA ATATTTACAC ACTGCAGATC 
AACCCAGATT CTTCTATCAA CCCTGACCAT CTGTCATACT TCCACTTTGT GGGTCGCATC 
ATGGGTCTGG CTGTGTTCCA CGGACACTAC ATAAATGGGG GTTTCACAGT TCCGTTCTAC 
AAGCAGCTCC TGGGGAAGCC AATCCAGCTG TCGGACCTGG AGTCCGTGGA CCCAGAACTG 
CATAAGAGCT TGGTGTGGAT TCTAGAGAAT GACATCACGC CTGTGTTGGA TCATACCTTC 
TGCGTGGAGC ACAACGCTTT CGGGCGGATT CTCCAGCATG AACTGAAACC CAATGGCAGA 
AATGTGCCTG TCACTGAGGA GAACAAGAAG GAATACGTCC GGCTGTATGT GAACTGGAGG 
TTTATGAGAG GAATCGAAGC CCAGTTCTTA GCACTTCAGA AGGGGTTTAA CGAACTCATT 
CCCCAACACT TGCTGAAGCC CTTTGACCAG AAGGAACTAG AGCTGATAAT AGGTGGGCTG 
GATAAGATAG ACCTGAACGA CTGGAAGTCC AACACCCGGC TGAAACACTG TGTGGCAGAC 
AGCAACATCG TCAGGTGGTT CTGGCAGGCG GTGGAGACCT TCGATGAGGA GAGGAGAGCC 
AGACTCCTGC AGTTTGTGAC AGGATCCACA AGAGTTCCTC TCCAAGGCTT CAAGGCTCTG 
CAAGGCTCTA CAGGCGCGGC AGGGCCCCGG CTGTTCACCA TTCACCTGAT AGACGCCAAT 
ACAGACAACC TGCCCAAGGC CCATACCTGC TTTAATCGGA TCGACATCCC ACCCTATGAG 
TCCTATGAGA AGCTCTATGA GAAGCTGCTG ACAGCGGTGG AGGAAACCTG TGGCTTTGCA 
GTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Smurf1
Entrez Gene ID	75788
Full Name	SMAD specific E3 ubiquitin protein ligase 1
Synonyms	4930431E10Rik,mKIAA1625
General protein information	Preferred Names SMAD specific E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase SMURF1 HECT-type E3 ubiquitin transferase SMURF1 Smad ubiquitination regulatory factor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2

Gallus gallus (chicken)	SMURF1	XP_414794.3
Homo sapiens (human)	SMURF1	NP_065162.1
	SMURF1	XP_002803251.1
Canis lupus familiaris (dog)	SMURF1	XP_005621234.1
Xenopus tropicalis (tropical clawed frog)	LOC100491582	XP_004917897.1
Pan troglodytes (chimpanzee)	SMURF1	XP_528043.3
Bos taurus (cattle)	SMURF1	XP_002698191.3
Mus musculus (house mouse)	Smurf1	NP_001033716.1
Rattus norvegicus (Norway rat)	Smurf1	NP_001103068.1
Danio rerio (zebrafish)	smurf1	NP_001001943.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Smurf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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