smurf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	smurf1
Entrez Gene ID	321695
Full Name	SMAD specific E3 ubiquitin protein ligase 1
Synonyms	wu:fb30b12,wwp1,zgc:66401
General protein information	Preferred Names SMAD specific E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase SMURF1 E3 ubiquitin ligase SMURF1 WW domain containing E3 ubiquitin protein ligase 1 smad ubiquitination regulatory factor 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021469369.1	XP_021325044.1	E3 ubiquitin-protein ligase SMURF1 isoform X2
XM_005164115.3	XP_005164172.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_005164115.4	XP_005164172.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
NM_001001943.1	NP_001001943.1	E3 ubiquitin-protein ligase SMURF1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SMURF1	XP_414794.3
Homo sapiens (human)	SMURF1	NP_065162.1
	SMURF1	XP_002803251.1
Canis lupus familiaris (dog)	SMURF1	XP_005621234.1
Xenopus tropicalis (tropical clawed frog)	LOC100491582	XP_004917897.1
Pan troglodytes (chimpanzee)	SMURF1	XP_528043.3
Bos taurus (cattle)	SMURF1	XP_002698191.3
Mus musculus (house mouse)	Smurf1	NP_001033716.1
Rattus norvegicus (Norway rat)	Smurf1	NP_001103068.1
Danio rerio (zebrafish)	smurf1	NP_001001943.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Itch is required for lateral line development in zebrafish.
Angers A, Drapeau P
PloS one9(11)e111799(2014)

Trb3 regulates LR axis formation in zebrafish embryos.
Anuppalle M, Maddirevula S, Huh TL, Rhee M
Molecules and cells36(6)542-7(2013 Dec)

SCFFBXL?? regulates BMP signalling by directing the degradation of HECT-type ubiquitin ligase Smurf1.
Cui Y, He S, Xing C, Lu K, Wang J, Xing G, Meng A, Jia S, He F, Zhang L
The EMBO journal30(13)2675-89(2011 May)

Smurf1 regulates neural patterning and folding in Xenopus embryos by antagonizing the BMP/Smad1 pathway.
Alexandrova EM, Thomsen GH
Developmental biology299(2)398-410(2006 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following smurf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smurf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36971	NM_001001943.1 Latest version!	Danio rerio SMAD specific E3 ubiquitin protein ligase 1 (smurf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa08575	XM_005164115.4 Latest version!	Danio rerio SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa141547	XM_021469369.1 Latest version!	Danio rerio SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa08575	XM_005164115.3	Danio rerio SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smurf1 ( NM_001001943.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001001943.1 Danio rerio SMAD specific E3 ubiquitin protein ligase 1 (smurf1), mRNA.

Price & Availability↑

CloneID	ODa36971
Clone ID Related Accession (Same CDS sequence)	NM_001001943.1
Accession Version	NM_001001943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase SMURF1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC059201.1. ##Evidence-Data-START## Transcript exon combination :: BC059201.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCGAATC CTGGGACTCG AAGGAACGGG TCAAGCATCA AAATCCGACT GACAGTATTA 
TGTGCCAAGA ATCTAGCAAA GAAAGACTTT TTCCGGTTGC CTGATCCTTT TGCCAAAGTG 
GTGGTGGACG GCTCGGGTCA ATGCCACTCT ACAGACACGG TCAAAAGCAC ACTGGACCCC 
AAGTGGAATC AGCATTACGA CCTGTATATT GGGAAGACCG ATTCCATCAC CATTAGCGTA 
TGGAACCATA AGAAGATCCA TAAGAAGCAG GGTGCGGGAT TCTTAGGCTG CGTGCGGCTC 
TTGTCAAACG CTATCAGCAG ACTAAAAGAC ACAGGCTACC AGCGTTTAGA CTTATGCAAG 
TTGAACCCAT CTGACAGTGA TGCTGTCCGT GGACAAATCG TTGTGAGTTT GCAGACCAGA 
GACAGAATTG GCAGTGGAGG GCCTGTGGTG GACTGTCGAG GACTGGTAGA GAATGAAGGG 
CCAGTCTTTG AAGACTCGGG ACCCGGACGG CCTCTCAGCT GCTTCATGGA GGAGCCCATG 
CCATACACCG ATCCCACAGG AGCCGCTGGA GGGGGCAACT GCCGTGCACT TGAGTCACCC 
AATCAGGAAC AACGGCTCCA AGCACAACGC ATCAGAAACC AGGATTCCCG AGGGCACACG 
CACACCCCCC AGAATCGACC GCACGGACAC CAATCCCCCG ACCTCCCTGA GGGCTATGAG 
CAAAGGACCA CCGTGCAGGG CCAAGTCTAT TTTCTGCACA CACAGACTGG CGTGAGCACC 
TGGCACGATC CCAGAATACC CCGGGATTTG AACAGCGTGA GTTGTGAGGA GCTGGGCCCA 
CTGCCGCCCG GCCGGGAGAT CAGGAGCACT GTGTCAGGAC GAATTTATTT CGTCGACCAC 
AATAATAGAA CCACGCAATT CACCGACCCC AGGTTACACC ACATCATGAG CCAACACTCT 
CAGGTGAAGG AGTCGAGCCA GGCTCTGCCC GTGCAGATGG AGGTTGGCAT GGAGGAGGGT 
GATGGAGAGT TGCCGGTGCG CTACGAAAGA GATCTTGTCC AGAAACTCAA AGTTCTCCGG 
CATGAGCTCT CGCTGCAGCA GCCCCAGGCC GGCCACTGCC GCATAGAAGT GTCCCGCGAG 
GAGATCTTTG AGGAGTCTTA TAGGCAGATC ATGAAGATGA GACCCAAAGA CCTGAAGAAG 
CGGCTGATGG TGAAGTTCAG AGGCGAGGAG GGGCTGGACT ACGGAGGAGT TGCTAGGGAG 
TGGTTATATC TGCTCTGCCA TGAGATGCTG AATCCATACT ATGGCTTGTT TCAGTACTCG 
ACCGATAACA TCTACACACT ACAGATCAAC CCAGACTCCT CCATTAACCC TGATCACTTG 
TCTTACTTCC ACTTTGTTGG GCGGATTATG GGTCTGGCTG TTTTCCACGG GCACTACATT 
AACGGGGGCT TCACCTTGCC CTTCTACAAG CAGCTCCTGG GGAAACCCAT TCAGCTCTGT 
GACCTTGAGA CCGTGGACCC AGAGCTGCAC AAGAGTCTCG TCTGGATCCT AGAAAATGAC 
ATCACATCTG TTTTGGACCA CACCTTCTGC GTAGAACATA ATGCTTTCGG CAAGTTCCTC 
CAACATGAGC TCAAACCAAA TGGCAAGAAC ATCCCAGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTGAGGC TGTACGTCAA CTGGAGGTTC ATGCGAGGCA TCGAGGCACA GTTTCTTGCC 
CTGCAAAAGG GCTTCAACGA ACTCATACCG CAGCATCTGC TCAAGCCTTT CGACAACAAG 
GAACTTGAGC TGATCATCGG TGGTTTGGGG AAGATCGATC TGAACGACTG GAAGGCCAAC 
ACGCGGCTGA AGCACTGTGT GGCTGACAGC AACATCGTGA AGTGGTTCTG GCAGGCGGTG 
GAGTCGTTTG ACGAGGAGAG GAGAGGCAGA CTCTTACAGT TCGTTACAGG CTCCACACGC 
GTCCCTCTAC AAGGCTTTAA AGCACTGCAA GGTTCTACAG GTTCTGCAGG ACCAAGACTC 
TTCACTATTC ATTTAATTGA TGCCAACACA GACAACCTGC CCAAAGCCCA CACATGTTTC 
AACCGTATCG ACATCCCGCC TTACGAGTCG TACGAGAAGC TGTATGAAAA GCTCCTGACA 
GCAGTGGAGG AGACATGTGG TTTCGCAGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001943.1
CDS	171..2366
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVKSTLDPKWNQHYDLYIGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDSDAVRGQIVVSLQTRDRIGSGGPVVDCRGLVENEGPVFEDSGPGRPLSCFMEEPMPYTDPTGAAGGGNCRALESPNQEQRLQAQRIRNQDSRGHTHTPQNRPHGHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVSCEELGPLPPGREIRSTVSGRIYFVDHNNRTTQFTDPRLHHIMSQHSQVKESSQALPVQMEVGMEEGDGELPVRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTLPFYKQLLGKPIQLCDLETVDPELHKSLVWILENDITSVLDHTFCVEHNAFGKFLQHELKPNGKNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDNKELELIIGGLGKIDLNDWKANTRLKHCVADSNIVKWFWQAVESFDEERRGRLLQFVTGSTRVPLQGFKALQGSTGSAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	NM_001001943.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMAD specific E3 ubiquitin protein ligase 1 (smurf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001943.1

ORF Insert Sequence:

ATGTCGAATC CTGGGACTCG AAGGAACGGG TCAAGCATCA AAATCCGACT GACAGTATTA 
TGTGCCAAGA ATCTAGCAAA GAAAGACTTT TTCCGGTTGC CTGATCCTTT TGCCAAAGTG 
GTGGTGGACG GCTCGGGTCA ATGCCACTCT ACAGACACGG TCAAAAGCAC ACTGGACCCC 
AAGTGGAATC AGCATTACGA CCTGTATATT GGGAAGACCG ATTCCATCAC CATTAGCGTA 
TGGAACCATA AGAAGATCCA TAAGAAGCAG GGTGCGGGAT TCTTAGGCTG CGTGCGGCTC 
TTGTCAAACG CTATCAGCAG ACTAAAAGAC ACAGGCTACC AGCGTTTAGA CTTATGCAAG 
TTGAACCCAT CTGACAGTGA TGCTGTCCGT GGACAAATCG TTGTGAGTTT GCAGACCAGA 
GACAGAATTG GCAGTGGAGG GCCTGTGGTG GACTGTCGAG GACTGGTAGA GAATGAAGGG 
CCAGTCTTTG AAGACTCGGG ACCCGGACGG CCTCTCAGCT GCTTCATGGA GGAGCCCATG 
CCATACACCG ATCCCACAGG AGCCGCTGGA GGGGGCAACT GCCGTGCACT TGAGTCACCC 
AATCAGGAAC AACGGCTCCA AGCACAACGC ATCAGAAACC AGGATTCCCG AGGGCACACG 
CACACCCCCC AGAATCGACC GCACGGACAC CAATCCCCCG ACCTCCCTGA GGGCTATGAG 
CAAAGGACCA CCGTGCAGGG CCAAGTCTAT TTTCTGCACA CACAGACTGG CGTGAGCACC 
TGGCACGATC CCAGAATACC CCGGGATTTG AACAGCGTGA GTTGTGAGGA GCTGGGCCCA 
CTGCCGCCCG GCCGGGAGAT CAGGAGCACT GTGTCAGGAC GAATTTATTT CGTCGACCAC 
AATAATAGAA CCACGCAATT CACCGACCCC AGGTTACACC ACATCATGAG CCAACACTCT 
CAGGTGAAGG AGTCGAGCCA GGCTCTGCCC GTGCAGATGG AGGTTGGCAT GGAGGAGGGT 
GATGGAGAGT TGCCGGTGCG CTACGAAAGA GATCTTGTCC AGAAACTCAA AGTTCTCCGG 
CATGAGCTCT CGCTGCAGCA GCCCCAGGCC GGCCACTGCC GCATAGAAGT GTCCCGCGAG 
GAGATCTTTG AGGAGTCTTA TAGGCAGATC ATGAAGATGA GACCCAAAGA CCTGAAGAAG 
CGGCTGATGG TGAAGTTCAG AGGCGAGGAG GGGCTGGACT ACGGAGGAGT TGCTAGGGAG 
TGGTTATATC TGCTCTGCCA TGAGATGCTG AATCCATACT ATGGCTTGTT TCAGTACTCG 
ACCGATAACA TCTACACACT ACAGATCAAC CCAGACTCCT CCATTAACCC TGATCACTTG 
TCTTACTTCC ACTTTGTTGG GCGGATTATG GGTCTGGCTG TTTTCCACGG GCACTACATT 
AACGGGGGCT TCACCTTGCC CTTCTACAAG CAGCTCCTGG GGAAACCCAT TCAGCTCTGT 
GACCTTGAGA CCGTGGACCC AGAGCTGCAC AAGAGTCTCG TCTGGATCCT AGAAAATGAC 
ATCACATCTG TTTTGGACCA CACCTTCTGC GTAGAACATA ATGCTTTCGG CAAGTTCCTC 
CAACATGAGC TCAAACCAAA TGGCAAGAAC ATCCCAGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTGAGGC TGTACGTCAA CTGGAGGTTC ATGCGAGGCA TCGAGGCACA GTTTCTTGCC 
CTGCAAAAGG GCTTCAACGA ACTCATACCG CAGCATCTGC TCAAGCCTTT CGACAACAAG 
GAACTTGAGC TGATCATCGG TGGTTTGGGG AAGATCGATC TGAACGACTG GAAGGCCAAC 
ACGCGGCTGA AGCACTGTGT GGCTGACAGC AACATCGTGA AGTGGTTCTG GCAGGCGGTG 
GAGTCGTTTG ACGAGGAGAG GAGAGGCAGA CTCTTACAGT TCGTTACAGG CTCCACACGC 
GTCCCTCTAC AAGGCTTTAA AGCACTGCAA GGTTCTACAG GTTCTGCAGG ACCAAGACTC 
TTCACTATTC ATTTAATTGA TGCCAACACA GACAACCTGC CCAAAGCCCA CACATGTTTC 
AACCGTATCG ACATCCCGCC TTACGAGTCG TACGAGAAGC TGTATGAAAA GCTCCTGACA 
GCAGTGGAGG AGACATGTGG TTTCGCAGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smurf1 ( XM_005164115.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164172.1 Danio rerio SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08575
Clone ID Related Accession (Same CDS sequence)	XM_005164115.3 , XM_005164115.4
Accession Version	XM_005164115.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164115.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAATC CTGGGACTCG AAGGAACGGG TCAAGCATCA AAATCCGACT GACAGTATTA 
TGTGCCAAGA ATCTAGCAAA GAAAGACTTC TTCCGGTTGC CTGATCCTTT TGCCAAAGTG 
GTGGTGGACG GCTCGGGTCA ATGCCACTCT ACAGACACGG TCAAAAGCAC ACTGGACCCC 
AAGTGGAATC AGCATTACGA CCTGTATATT GGGAAGACCG ATTCCATCAC CATTAGCGTA 
TGGAACCATA AGAAGATCCA TAAGAAGCAG GGTGCGGGAT TCTTAGGCTG CGTGCGGCTC 
TTGTCAAACG CTATCAGCAG ACTAAAAGAC ACAGGCTACC AGCGTTTAGA CTTATGCAAG 
TTAAACCCAT CTGACAGTGA TGCTGTCCGT GGACAAATCG TTGTGAGTTT GCAGACCAGA 
GACAGAATTG GCAGTGGAGG GCCTGTGGTG GACTGTCGAG GACTGGTAGA GAATGAAGGG 
CCAGTCTTTG AAGACTCGGG ACCCGGACGG CCTCTCAGCT GCTTCATGGA GGAGCCCATG 
CCATACACCG ATCCCACAGG AGCCGCTGGA GGGGGCAACT GCCGTGCACT TGAGTCACCC 
AATCAGGAAC AACGGCTCCA AGCACAACGC ATCAGAAACC AGGATTCCCG AGGGCACACG 
CACACCCCCC AGAATCGACC GCACGGACAC CAATCCCCCG ACCTCCCTGA GGGCTATGAG 
CAAAGGACCA CCGTGCAGGG CCAAGTCTAT TTTCTGCACA CACAGACTGG CGTGAGCACC 
TGGCACGATC CCAGAATACC CCGGGATTTG AACAGCGTGA GTTGTGAGGA GCTGGGCCCA 
CTGCCGCCCG GCTGGGAGAT CAGGAGCACT GTGTCAGGAC GAATTTATTT CGTCGACCAC 
AATAATAGAA CCACGCAATT CACCGACCCC AGGTTACACC ACATCATGAG CCAACACTCT 
CAGGTGAAGG AGTCGAGCCA GGCTCTGCCC GTGCAGATGG AGGTTGGCAT GGAGGAGGGT 
GATGGAGAGT TGCCGGTGCG CTACGAAAGA GATCTTGTCC AGAAACTCAA AGTTCTCCGG 
CATGAGCTCT CGCTGCAGCA GCCTCAGGCC GGCCACTGCC GCATAGAAGT GTCCCGCGAG 
GAGATCTTTG AGGAGTCTTA TAGGCAGATC ATGAAGATGA GACCCAAAGA CCTGAAGAAG 
CGGCTGATGG TGAAGTTCAG AGGCGAGGAG GGGCTGGATT ACGGAGGAGT TGCTAGGGAG 
TGGTTATATC TGCTCTGCCA TGAGATGCTG AATCCATACT ATGGCTTGTT TCAGTACTCG 
ACCGATAACA TCTACACACT ACAGATCAAC CCAGACTCCT CCATTAACCC TGATCACTTG 
TCTTACTTCC ACTTTGTTGG GCGGATTATG GGTCTGGCTG TTTTCCACGG GCACTACATT 
AACGGGGGCT TCACCTTGCC CTTCTACAAG CAGCTCCTGG GGAAACCCAT TCAGCTTTGT 
GACCTTGAGA CCGTGGACCC AGAGCTGCAC AAGAGTCTCG TCTGGATCCT AGAAAATGAC 
ATCACATCTG TTTTGGACCA CACCTTCTGC GTAGAACATA ATGCTTTCGG CAAGTTCCTC 
CAACATGAGC TCAAACCAAA TGGCAAGAAC ATCCCAGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTGAGGC TGTACGTCAA CTGGAGGTTC ATGCGAGGCA TCGAGGCACA GTTTCTTGCC 
CTGCAAAAGG GCTTCAACGA ACTCATACCG CAGCATCTGC TCAAGCCTTT CGACAACAAG 
GAACTTGAGC TGATCATCGG TGGTTTGGGG AAGATCGATC TGAACGACTG GAAGGCCAAC 
ACGCGGCTGA AGCACTGTGT GGCTGACAGC AACATCGTGA AGTGGTTTTG GCAGGCGGTG 
GAGTCGTTTG ACGAGGAGAG GAGAGGCAGA CTCTTACAGT TCGTGACAGG CTCCACACGC 
GTCCCTCTAC AAGGCTTTAA AGCACTGCAA GGTTCTGCAG GACCAAGACT CTTCACTATT 
CATTTAATTG ATGCCAACAC AGACAACCTG CCCAAAGCCC ACACATGTTT CAACCGTATC 
GACATCCCGC CTTACGAGTC GTACGAGAAG CTGTATGAAA AGCTCCTGAC AGCAGTGGAG 
GAGACATGTG GTTTCGCAGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164172.1
CDS	255..2441
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVKSTLDPKWNQHYDLYIGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDSDAVRGQIVVSLQTRDRIGSGGPVVDCRGLVENEGPVFEDSGPGRPLSCFMEEPMPYTDPTGAAGGGNCRALESPNQEQRLQAQRIRNQDSRGHTHTPQNRPHGHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVSCEELGPLPPGWEIRSTVSGRIYFVDHNNRTTQFTDPRLHHIMSQHSQVKESSQALPVQMEVGMEEGDGELPVRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTLPFYKQLLGKPIQLCDLETVDPELHKSLVWILENDITSVLDHTFCVEHNAFGKFLQHELKPNGKNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDNKELELIIGGLGKIDLNDWKANTRLKHCVADSNIVKWFWQAVESFDEERRGRLLQFVTGSTRVPLQGFKALQGSAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_005164115.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164115.3

ORF Insert Sequence:

ATGTCGAATC CTGGGACTCG AAGGAACGGG TCAAGCATCA AAATCCGACT GACAGTATTA 
TGTGCCAAGA ATCTAGCAAA GAAAGACTTC TTCCGGTTGC CTGATCCTTT TGCCAAAGTG 
GTGGTGGACG GCTCGGGTCA ATGCCACTCT ACAGACACGG TCAAAAGCAC ACTGGACCCC 
AAGTGGAATC AGCATTACGA CCTGTATATT GGGAAGACCG ATTCCATCAC CATTAGCGTA 
TGGAACCATA AGAAGATCCA TAAGAAGCAG GGTGCGGGAT TCTTAGGCTG CGTGCGGCTC 
TTGTCAAACG CTATCAGCAG ACTAAAAGAC ACAGGCTACC AGCGTTTAGA CTTATGCAAG 
TTAAACCCAT CTGACAGTGA TGCTGTCCGT GGACAAATCG TTGTGAGTTT GCAGACCAGA 
GACAGAATTG GCAGTGGAGG GCCTGTGGTG GACTGTCGAG GACTGGTAGA GAATGAAGGG 
CCAGTCTTTG AAGACTCGGG ACCCGGACGG CCTCTCAGCT GCTTCATGGA GGAGCCCATG 
CCATACACCG ATCCCACAGG AGCCGCTGGA GGGGGCAACT GCCGTGCACT TGAGTCACCC 
AATCAGGAAC AACGGCTCCA AGCACAACGC ATCAGAAACC AGGATTCCCG AGGGCACACG 
CACACCCCCC AGAATCGACC GCACGGACAC CAATCCCCCG ACCTCCCTGA GGGCTATGAG 
CAAAGGACCA CCGTGCAGGG CCAAGTCTAT TTTCTGCACA CACAGACTGG CGTGAGCACC 
TGGCACGATC CCAGAATACC CCGGGATTTG AACAGCGTGA GTTGTGAGGA GCTGGGCCCA 
CTGCCGCCCG GCTGGGAGAT CAGGAGCACT GTGTCAGGAC GAATTTATTT CGTCGACCAC 
AATAATAGAA CCACGCAATT CACCGACCCC AGGTTACACC ACATCATGAG CCAACACTCT 
CAGGTGAAGG AGTCGAGCCA GGCTCTGCCC GTGCAGATGG AGGTTGGCAT GGAGGAGGGT 
GATGGAGAGT TGCCGGTGCG CTACGAAAGA GATCTTGTCC AGAAACTCAA AGTTCTCCGG 
CATGAGCTCT CGCTGCAGCA GCCTCAGGCC GGCCACTGCC GCATAGAAGT GTCCCGCGAG 
GAGATCTTTG AGGAGTCTTA TAGGCAGATC ATGAAGATGA GACCCAAAGA CCTGAAGAAG 
CGGCTGATGG TGAAGTTCAG AGGCGAGGAG GGGCTGGATT ACGGAGGAGT TGCTAGGGAG 
TGGTTATATC TGCTCTGCCA TGAGATGCTG AATCCATACT ATGGCTTGTT TCAGTACTCG 
ACCGATAACA TCTACACACT ACAGATCAAC CCAGACTCCT CCATTAACCC TGATCACTTG 
TCTTACTTCC ACTTTGTTGG GCGGATTATG GGTCTGGCTG TTTTCCACGG GCACTACATT 
AACGGGGGCT TCACCTTGCC CTTCTACAAG CAGCTCCTGG GGAAACCCAT TCAGCTTTGT 
GACCTTGAGA CCGTGGACCC AGAGCTGCAC AAGAGTCTCG TCTGGATCCT AGAAAATGAC 
ATCACATCTG TTTTGGACCA CACCTTCTGC GTAGAACATA ATGCTTTCGG CAAGTTCCTC 
CAACATGAGC TCAAACCAAA TGGCAAGAAC ATCCCAGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTGAGGC TGTACGTCAA CTGGAGGTTC ATGCGAGGCA TCGAGGCACA GTTTCTTGCC 
CTGCAAAAGG GCTTCAACGA ACTCATACCG CAGCATCTGC TCAAGCCTTT CGACAACAAG 
GAACTTGAGC TGATCATCGG TGGTTTGGGG AAGATCGATC TGAACGACTG GAAGGCCAAC 
ACGCGGCTGA AGCACTGTGT GGCTGACAGC AACATCGTGA AGTGGTTTTG GCAGGCGGTG 
GAGTCGTTTG ACGAGGAGAG GAGAGGCAGA CTCTTACAGT TCGTGACAGG CTCCACACGC 
GTCCCTCTAC AAGGCTTTAA AGCACTGCAA GGTTCTGCAG GACCAAGACT CTTCACTATT 
CATTTAATTG ATGCCAACAC AGACAACCTG CCCAAAGCCC ACACATGTTT CAACCGTATC 
GACATCCCGC CTTACGAGTC GTACGAGAAG CTGTATGAAA AGCTCCTGAC AGCAGTGGAG 
GAGACATGTG GTTTCGCAGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smurf1 ( XM_005164115.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164172.1 Danio rerio SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08575
Clone ID Related Accession (Same CDS sequence)	XM_005164115.3 , XM_005164115.4
Accession Version	XM_005164115.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164115.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAATC CTGGGACTCG AAGGAACGGG TCAAGCATCA AAATCCGACT GACAGTATTA 
TGTGCCAAGA ATCTAGCAAA GAAAGACTTC TTCCGGTTGC CTGATCCTTT TGCCAAAGTG 
GTGGTGGACG GCTCGGGTCA ATGCCACTCT ACAGACACGG TCAAAAGCAC ACTGGACCCC 
AAGTGGAATC AGCATTACGA CCTGTATATT GGGAAGACCG ATTCCATCAC CATTAGCGTA 
TGGAACCATA AGAAGATCCA TAAGAAGCAG GGTGCGGGAT TCTTAGGCTG CGTGCGGCTC 
TTGTCAAACG CTATCAGCAG ACTAAAAGAC ACAGGCTACC AGCGTTTAGA CTTATGCAAG 
TTAAACCCAT CTGACAGTGA TGCTGTCCGT GGACAAATCG TTGTGAGTTT GCAGACCAGA 
GACAGAATTG GCAGTGGAGG GCCTGTGGTG GACTGTCGAG GACTGGTAGA GAATGAAGGG 
CCAGTCTTTG AAGACTCGGG ACCCGGACGG CCTCTCAGCT GCTTCATGGA GGAGCCCATG 
CCATACACCG ATCCCACAGG AGCCGCTGGA GGGGGCAACT GCCGTGCACT TGAGTCACCC 
AATCAGGAAC AACGGCTCCA AGCACAACGC ATCAGAAACC AGGATTCCCG AGGGCACACG 
CACACCCCCC AGAATCGACC GCACGGACAC CAATCCCCCG ACCTCCCTGA GGGCTATGAG 
CAAAGGACCA CCGTGCAGGG CCAAGTCTAT TTTCTGCACA CACAGACTGG CGTGAGCACC 
TGGCACGATC CCAGAATACC CCGGGATTTG AACAGCGTGA GTTGTGAGGA GCTGGGCCCA 
CTGCCGCCCG GCTGGGAGAT CAGGAGCACT GTGTCAGGAC GAATTTATTT CGTCGACCAC 
AATAATAGAA CCACGCAATT CACCGACCCC AGGTTACACC ACATCATGAG CCAACACTCT 
CAGGTGAAGG AGTCGAGCCA GGCTCTGCCC GTGCAGATGG AGGTTGGCAT GGAGGAGGGT 
GATGGAGAGT TGCCGGTGCG CTACGAAAGA GATCTTGTCC AGAAACTCAA AGTTCTCCGG 
CATGAGCTCT CGCTGCAGCA GCCTCAGGCC GGCCACTGCC GCATAGAAGT GTCCCGCGAG 
GAGATCTTTG AGGAGTCTTA TAGGCAGATC ATGAAGATGA GACCCAAAGA CCTGAAGAAG 
CGGCTGATGG TGAAGTTCAG AGGCGAGGAG GGGCTGGATT ACGGAGGAGT TGCTAGGGAG 
TGGTTATATC TGCTCTGCCA TGAGATGCTG AATCCATACT ATGGCTTGTT TCAGTACTCG 
ACCGATAACA TCTACACACT ACAGATCAAC CCAGACTCCT CCATTAACCC TGATCACTTG 
TCTTACTTCC ACTTTGTTGG GCGGATTATG GGTCTGGCTG TTTTCCACGG GCACTACATT 
AACGGGGGCT TCACCTTGCC CTTCTACAAG CAGCTCCTGG GGAAACCCAT TCAGCTTTGT 
GACCTTGAGA CCGTGGACCC AGAGCTGCAC AAGAGTCTCG TCTGGATCCT AGAAAATGAC 
ATCACATCTG TTTTGGACCA CACCTTCTGC GTAGAACATA ATGCTTTCGG CAAGTTCCTC 
CAACATGAGC TCAAACCAAA TGGCAAGAAC ATCCCAGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTGAGGC TGTACGTCAA CTGGAGGTTC ATGCGAGGCA TCGAGGCACA GTTTCTTGCC 
CTGCAAAAGG GCTTCAACGA ACTCATACCG CAGCATCTGC TCAAGCCTTT CGACAACAAG 
GAACTTGAGC TGATCATCGG TGGTTTGGGG AAGATCGATC TGAACGACTG GAAGGCCAAC 
ACGCGGCTGA AGCACTGTGT GGCTGACAGC AACATCGTGA AGTGGTTTTG GCAGGCGGTG 
GAGTCGTTTG ACGAGGAGAG GAGAGGCAGA CTCTTACAGT TCGTGACAGG CTCCACACGC 
GTCCCTCTAC AAGGCTTTAA AGCACTGCAA GGTTCTGCAG GACCAAGACT CTTCACTATT 
CATTTAATTG ATGCCAACAC AGACAACCTG CCCAAAGCCC ACACATGTTT CAACCGTATC 
GACATCCCGC CTTACGAGTC GTACGAGAAG CTGTATGAAA AGCTCCTGAC AGCAGTGGAG 
GAGACATGTG GTTTCGCAGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164172.1
CDS	236..2422
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVKSTLDPKWNQHYDLYIGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDSDAVRGQIVVSLQTRDRIGSGGPVVDCRGLVENEGPVFEDSGPGRPLSCFMEEPMPYTDPTGAAGGGNCRALESPNQEQRLQAQRIRNQDSRGHTHTPQNRPHGHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVSCEELGPLPPGWEIRSTVSGRIYFVDHNNRTTQFTDPRLHHIMSQHSQVKESSQALPVQMEVGMEEGDGELPVRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTLPFYKQLLGKPIQLCDLETVDPELHKSLVWILENDITSVLDHTFCVEHNAFGKFLQHELKPNGKNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDNKELELIIGGLGKIDLNDWKANTRLKHCVADSNIVKWFWQAVESFDEERRGRLLQFVTGSTRVPLQGFKALQGSAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_005164115.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164115.4

ORF Insert Sequence:

ATGTCGAATC CTGGGACTCG AAGGAACGGG TCAAGCATCA AAATCCGACT GACAGTATTA 
TGTGCCAAGA ATCTAGCAAA GAAAGACTTC TTCCGGTTGC CTGATCCTTT TGCCAAAGTG 
GTGGTGGACG GCTCGGGTCA ATGCCACTCT ACAGACACGG TCAAAAGCAC ACTGGACCCC 
AAGTGGAATC AGCATTACGA CCTGTATATT GGGAAGACCG ATTCCATCAC CATTAGCGTA 
TGGAACCATA AGAAGATCCA TAAGAAGCAG GGTGCGGGAT TCTTAGGCTG CGTGCGGCTC 
TTGTCAAACG CTATCAGCAG ACTAAAAGAC ACAGGCTACC AGCGTTTAGA CTTATGCAAG 
TTAAACCCAT CTGACAGTGA TGCTGTCCGT GGACAAATCG TTGTGAGTTT GCAGACCAGA 
GACAGAATTG GCAGTGGAGG GCCTGTGGTG GACTGTCGAG GACTGGTAGA GAATGAAGGG 
CCAGTCTTTG AAGACTCGGG ACCCGGACGG CCTCTCAGCT GCTTCATGGA GGAGCCCATG 
CCATACACCG ATCCCACAGG AGCCGCTGGA GGGGGCAACT GCCGTGCACT TGAGTCACCC 
AATCAGGAAC AACGGCTCCA AGCACAACGC ATCAGAAACC AGGATTCCCG AGGGCACACG 
CACACCCCCC AGAATCGACC GCACGGACAC CAATCCCCCG ACCTCCCTGA GGGCTATGAG 
CAAAGGACCA CCGTGCAGGG CCAAGTCTAT TTTCTGCACA CACAGACTGG CGTGAGCACC 
TGGCACGATC CCAGAATACC CCGGGATTTG AACAGCGTGA GTTGTGAGGA GCTGGGCCCA 
CTGCCGCCCG GCTGGGAGAT CAGGAGCACT GTGTCAGGAC GAATTTATTT CGTCGACCAC 
AATAATAGAA CCACGCAATT CACCGACCCC AGGTTACACC ACATCATGAG CCAACACTCT 
CAGGTGAAGG AGTCGAGCCA GGCTCTGCCC GTGCAGATGG AGGTTGGCAT GGAGGAGGGT 
GATGGAGAGT TGCCGGTGCG CTACGAAAGA GATCTTGTCC AGAAACTCAA AGTTCTCCGG 
CATGAGCTCT CGCTGCAGCA GCCTCAGGCC GGCCACTGCC GCATAGAAGT GTCCCGCGAG 
GAGATCTTTG AGGAGTCTTA TAGGCAGATC ATGAAGATGA GACCCAAAGA CCTGAAGAAG 
CGGCTGATGG TGAAGTTCAG AGGCGAGGAG GGGCTGGATT ACGGAGGAGT TGCTAGGGAG 
TGGTTATATC TGCTCTGCCA TGAGATGCTG AATCCATACT ATGGCTTGTT TCAGTACTCG 
ACCGATAACA TCTACACACT ACAGATCAAC CCAGACTCCT CCATTAACCC TGATCACTTG 
TCTTACTTCC ACTTTGTTGG GCGGATTATG GGTCTGGCTG TTTTCCACGG GCACTACATT 
AACGGGGGCT TCACCTTGCC CTTCTACAAG CAGCTCCTGG GGAAACCCAT TCAGCTTTGT 
GACCTTGAGA CCGTGGACCC AGAGCTGCAC AAGAGTCTCG TCTGGATCCT AGAAAATGAC 
ATCACATCTG TTTTGGACCA CACCTTCTGC GTAGAACATA ATGCTTTCGG CAAGTTCCTC 
CAACATGAGC TCAAACCAAA TGGCAAGAAC ATCCCAGTCA CAGAGGAGAA CAAGAAAGAA 
TACGTGAGGC TGTACGTCAA CTGGAGGTTC ATGCGAGGCA TCGAGGCACA GTTTCTTGCC 
CTGCAAAAGG GCTTCAACGA ACTCATACCG CAGCATCTGC TCAAGCCTTT CGACAACAAG 
GAACTTGAGC TGATCATCGG TGGTTTGGGG AAGATCGATC TGAACGACTG GAAGGCCAAC 
ACGCGGCTGA AGCACTGTGT GGCTGACAGC AACATCGTGA AGTGGTTTTG GCAGGCGGTG 
GAGTCGTTTG ACGAGGAGAG GAGAGGCAGA CTCTTACAGT TCGTGACAGG CTCCACACGC 
GTCCCTCTAC AAGGCTTTAA AGCACTGCAA GGTTCTGCAG GACCAAGACT CTTCACTATT 
CATTTAATTG ATGCCAACAC AGACAACCTG CCCAAAGCCC ACACATGTTT CAACCGTATC 
GACATCCCGC CTTACGAGTC GTACGAGAAG CTGTATGAAA AGCTCCTGAC AGCAGTGGAG 
GAGACATGTG GTTTCGCAGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smurf1 ( XM_021469369.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325044.1 Danio rerio SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa141547
Clone ID Related Accession (Same CDS sequence)	XM_021469369.1
Accession Version	XM_021469369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAATC CTGGGACTCG AAGGAACGGG TCAAGCATCA AAATCCGACT GACAGTATTA 
TGTGCCAAGA ATCTAGCAAA GAAAGACTTC TTCCGGTTGC CTGATCCTTT TGCCAAAGTG 
GTGGTGGACG GCTCGGGTCA ATGCCACTCT ACAGACACGG TCAAAAGCAC ACTGGACCCC 
AAGTGGAATC AGCATTACGA CCTGTATATT GGGAAGACCG ATTCCATCAC CATTAGCGTA 
TGGAACCATA AGAAGATCCA TAAGAAGCAG GGTGCGGGAT TCTTAGGCTG CGTGCGGCTC 
TTGTCAAACG CTATCAGCAG ACTAAAAGAC ACAGGCTACC AGCGTTTAGA CTTATGCAAG 
TTAAACCCAT CTGACAGTGA TGCTGTCCGT GGACAAATCG TTGTGAGTTT GCAGACCAGA 
GACAGAATTG GCAGTGGAGG GCCTGTGGTG GACTGTCGAG GACTGGTAGA GAATGAAGGG 
CCAGTCTTTG AAGACTCGGG ACCCGGACGG CCTCTCAGCT GCTTCATGGA GGAGCCCATG 
CCATACACCG ATCCCACAGG AGCCGCTGGA GGGGGCAACT GCCGTGCACT TGAGTCACCC 
AATCAGGAAC AACGGCTCCA AGCACAACGC ATCAGAAACC AGGATTCCCG AGGGCACACG 
CACACCCCCC AGAATCGACC GCACGGACAC CAATCCCCCG ACCTCCCTGA GGGCTATGAG 
CAAAGGACCA CCGTGCAGGG CCAAGTCTAT TTTCTGCACA CACAGACTGG CGTGAGCACC 
TGGCACGATC CCAGAATACC CCGCCAACAC TCTCAGGTGA AGGAGTCGAG CCAGGCTCTG 
CCCGTGCAGA TGGAGGTTGG CATGGAGGAG GGTGATGGAG AGTTGCCGGT GCGCTACGAA 
AGAGATCTTG TCCAGAAACT CAAAGTTCTC CGGCATGAGC TCTCGCTGCA GCAGCCTCAG 
GCCGGCCACT GCCGCATAGA AGTGTCCCGC GAGGAGATCT TTGAGGAGTC TTATAGGCAG 
ATCATGAAGA TGAGACCCAA AGACCTGAAG AAGCGGCTGA TGGTGAAGTT CAGAGGCGAG 
GAGGGGCTGG ATTACGGAGG AGTTGCTAGG GAGTGGTTAT ATCTGCTCTG CCATGAGATG 
CTGAATCCAT ACTATGGCTT GTTTCAGTAC TCGACCGATA ACATCTACAC ACTACAGATC 
AACCCAGACT CCTCCATTAA CCCTGATCAC TTGTCTTACT TCCACTTTGT TGGGCGGATT 
ATGGGTCTGG CTGTTTTCCA CGGGCACTAC ATTAACGGGG GCTTCACCTT GCCCTTCTAC 
AAGCAGCTCC TGGGGAAACC CATTCAGCTT TGTGACCTTG AGACCGTGGA CCCAGAGCTG 
CACAAGAGTC TCGTCTGGAT CCTAGAAAAT GACATCACAT CTGTTTTGGA CCACACCTTC 
TGCGTAGAAC ATAATGCTTT CGGCAAGTTC CTCCAACATG AGCTCAAACC AAATGGCAAG 
AACATCCCAG TCACAGAGGA GAACAAGAAA GAATACGTGA GGCTGTACGT CAACTGGAGG 
TTCATGCGAG GCATCGAGGC ACAGTTTCTT GCCCTGCAAA AGGGCTTCAA CGAACTCATA 
CCGCAGCATC TGCTCAAGCC TTTCGACAAC AAGGAACTTG AGCTGATCAT CGGTGGTTTG 
GGGAAGATCG ATCTGAACGA CTGGAAGGCC AACACGCGGC TGAAGCACTG TGTGGCTGAC 
AGCAACATCG TGAAGTGGTT TTGGCAGGCG GTGGAGTCGT TTGACGAGGA GAGGAGAGGC 
AGACTCTTAC AGTTCGTGAC AGGCTCCACA CGCGTCCCTC TACAAGGCTT TAAAGCACTG 
CAAGGTTCTA CAGGTTCTGC AGGACCAAGA CTCTTCACTA TTCATTTAAT TGATGCCAAC 
ACAGACAACC TGCCCAAAGC CCACACATGT TTCAACCGTA TCGACATCCC GCCTTACGAG 
TCGTACGAGA AGCTGTATGA AAAGCTCCTG ACAGCAGTGG AGGAGACATG TGGTTTCGCA 
GTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325044.1
CDS	236..2284
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVKSTLDPKWNQHYDLYIGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDSDAVRGQIVVSLQTRDRIGSGGPVVDCRGLVENEGPVFEDSGPGRPLSCFMEEPMPYTDPTGAAGGGNCRALESPNQEQRLQAQRIRNQDSRGHTHTPQNRPHGHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRQHSQVKESSQALPVQMEVGMEEGDGELPVRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTLPFYKQLLGKPIQLCDLETVDPELHKSLVWILENDITSVLDHTFCVEHNAFGKFLQHELKPNGKNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDNKELELIIGGLGKIDLNDWKANTRLKHCVADSNIVKWFWQAVESFDEERRGRLLQFVTGSTRVPLQGFKALQGSTGSAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_021469369.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469369.1

ORF Insert Sequence:

ATGTCGAATC CTGGGACTCG AAGGAACGGG TCAAGCATCA AAATCCGACT GACAGTATTA 
TGTGCCAAGA ATCTAGCAAA GAAAGACTTC TTCCGGTTGC CTGATCCTTT TGCCAAAGTG 
GTGGTGGACG GCTCGGGTCA ATGCCACTCT ACAGACACGG TCAAAAGCAC ACTGGACCCC 
AAGTGGAATC AGCATTACGA CCTGTATATT GGGAAGACCG ATTCCATCAC CATTAGCGTA 
TGGAACCATA AGAAGATCCA TAAGAAGCAG GGTGCGGGAT TCTTAGGCTG CGTGCGGCTC 
TTGTCAAACG CTATCAGCAG ACTAAAAGAC ACAGGCTACC AGCGTTTAGA CTTATGCAAG 
TTAAACCCAT CTGACAGTGA TGCTGTCCGT GGACAAATCG TTGTGAGTTT GCAGACCAGA 
GACAGAATTG GCAGTGGAGG GCCTGTGGTG GACTGTCGAG GACTGGTAGA GAATGAAGGG 
CCAGTCTTTG AAGACTCGGG ACCCGGACGG CCTCTCAGCT GCTTCATGGA GGAGCCCATG 
CCATACACCG ATCCCACAGG AGCCGCTGGA GGGGGCAACT GCCGTGCACT TGAGTCACCC 
AATCAGGAAC AACGGCTCCA AGCACAACGC ATCAGAAACC AGGATTCCCG AGGGCACACG 
CACACCCCCC AGAATCGACC GCACGGACAC CAATCCCCCG ACCTCCCTGA GGGCTATGAG 
CAAAGGACCA CCGTGCAGGG CCAAGTCTAT TTTCTGCACA CACAGACTGG CGTGAGCACC 
TGGCACGATC CCAGAATACC CCGCCAACAC TCTCAGGTGA AGGAGTCGAG CCAGGCTCTG 
CCCGTGCAGA TGGAGGTTGG CATGGAGGAG GGTGATGGAG AGTTGCCGGT GCGCTACGAA 
AGAGATCTTG TCCAGAAACT CAAAGTTCTC CGGCATGAGC TCTCGCTGCA GCAGCCTCAG 
GCCGGCCACT GCCGCATAGA AGTGTCCCGC GAGGAGATCT TTGAGGAGTC TTATAGGCAG 
ATCATGAAGA TGAGACCCAA AGACCTGAAG AAGCGGCTGA TGGTGAAGTT CAGAGGCGAG 
GAGGGGCTGG ATTACGGAGG AGTTGCTAGG GAGTGGTTAT ATCTGCTCTG CCATGAGATG 
CTGAATCCAT ACTATGGCTT GTTTCAGTAC TCGACCGATA ACATCTACAC ACTACAGATC 
AACCCAGACT CCTCCATTAA CCCTGATCAC TTGTCTTACT TCCACTTTGT TGGGCGGATT 
ATGGGTCTGG CTGTTTTCCA CGGGCACTAC ATTAACGGGG GCTTCACCTT GCCCTTCTAC 
AAGCAGCTCC TGGGGAAACC CATTCAGCTT TGTGACCTTG AGACCGTGGA CCCAGAGCTG 
CACAAGAGTC TCGTCTGGAT CCTAGAAAAT GACATCACAT CTGTTTTGGA CCACACCTTC 
TGCGTAGAAC ATAATGCTTT CGGCAAGTTC CTCCAACATG AGCTCAAACC AAATGGCAAG 
AACATCCCAG TCACAGAGGA GAACAAGAAA GAATACGTGA GGCTGTACGT CAACTGGAGG 
TTCATGCGAG GCATCGAGGC ACAGTTTCTT GCCCTGCAAA AGGGCTTCAA CGAACTCATA 
CCGCAGCATC TGCTCAAGCC TTTCGACAAC AAGGAACTTG AGCTGATCAT CGGTGGTTTG 
GGGAAGATCG ATCTGAACGA CTGGAAGGCC AACACGCGGC TGAAGCACTG TGTGGCTGAC 
AGCAACATCG TGAAGTGGTT TTGGCAGGCG GTGGAGTCGT TTGACGAGGA GAGGAGAGGC 
AGACTCTTAC AGTTCGTGAC AGGCTCCACA CGCGTCCCTC TACAAGGCTT TAAAGCACTG 
CAAGGTTCTA CAGGTTCTGC AGGACCAAGA CTCTTCACTA TTCATTTAAT TGATGCCAAC 
ACAGACAACC TGCCCAAAGC CCACACATGT TTCAACCGTA TCGACATCCC GCCTTACGAG 
TCGTACGAGA AGCTGTATGA AAAGCTCCTG ACAGCAGTGG AGGAGACATG TGGTTTCGCA 
GTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Itch is required for lateral line development in zebrafish.
PloS one9(11)e111799(2014)
Angers A,Drapeau P

Trb3 regulates LR axis formation in zebrafish embryos.
Molecules and cells36(6)542-7(2013 Dec)
Anuppalle M,Maddirevula S,Huh TL,Rhee M

SCFFBXL?? regulates BMP signalling by directing the degradation of HECT-type ubiquitin ligase Smurf1.
The EMBO journal30(13)2675-89(2011 May)
Cui Y,He S,Xing C,Lu K,Wang J,Xing G,Meng A,Jia S,He F,Zhang L

Smurf1 regulates neural patterning and folding in Xenopus embryos by antagonizing the BMP/Smad1 pathway.
Developmental biology299(2)398-410(2006 Nov)
Alexandrova EM,Thomsen GH

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smurf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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