NEK1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEK1
Entrez Gene ID	471345
Full Name	NIMA related kinase 1
General protein information	Preferred Names NIMA related kinase 1 Names serine/threonine-protein kinase Nek1 NIMA (never in mitosis gene a)-related kinase 1
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_526727.5	XP_526727.3	serine/threonine-protein kinase Nek1 isoform X1
XM_001153290.4	XP_001153290.2	serine/threonine-protein kinase Nek1 isoform X3
XM_009448565.2	XP_009446840.2	serine/threonine-protein kinase Nek1 isoform X2
XM_009448566.2	XP_009446841.2	serine/threonine-protein kinase Nek1 isoform X4
XM_009448565.3	XP_009446840.2	serine/threonine-protein kinase Nek1 isoform X2
XM_009448566.3	XP_009446841.2	serine/threonine-protein kinase Nek1 isoform X4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NEK1	XP_003586402.2
Rattus norvegicus (Norway rat)	Nek1	NP_001099552.2
Gallus gallus (chicken)	NEK1	XP_420401.3
Homo sapiens (human)	NEK1	NP_001186326.1
	NEK1	XP_001083275.1
Canis lupus familiaris (dog)	NEK1	XP_543184.2
Mus musculus (house mouse)	Nek1	NP_780298.2
Pan troglodytes (chimpanzee)	NEK1	XP_526727.2
Danio rerio (zebrafish)	nek1	XP_005160601.1
Xenopus tropicalis (tropical clawed frog)	nek1	NP_001090680.1

Gene Ontology

The following NEK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc84409	XM_526727.5 Latest version!	Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPc84410	XM_009448565.3 Latest version!	Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPc84411	XM_001153290.4 Latest version!	Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPc84412	XM_009448566.3 Latest version!	Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OPc84410	XM_009448565.2	Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPc84412	XM_009448566.2	Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEK1 ( XM_526727.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_526727.3 Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc84409
Clone ID Related Accession (Same CDS sequence)	XM_526727.5
Accession Version	XM_526727.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_526727.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT GGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCAAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ACGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATACC 
CGGGAAACTT CAGAAGAGAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGAATCTC 
TCTGATACTT GTGAGATAAA TGTTCATGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGGTCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTA 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGAGA TTTTTCCCGA AGGGGAAAAG TACAAACCCT TAATTACTGG AGAAAAAAAA 
GTACAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGTCCCG AGTTCAGTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAATT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CGTGCACTAT TACTGATGTG TGGATTAGTG AGGAAAAAGA AACAAAGGAA 
ACTCAGTCGG CAGATAGGAT CACCATTCAG GAAAATGAAG TTTCTGAAGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATAGAGCCTG GAACCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAACCAT TTTTCCATAA GGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGACAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCG 
ATGGAACAGT TGCTTAGGGA ACAACCTGGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTGAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCAGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGTGAA 
ATTGCTAGTG AATGTGAATG TGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAACAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ATGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_526727.3
CDS	581..4441
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTCEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEIFPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLDIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_526727.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_526727.5

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT GGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCAAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ACGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATACC 
CGGGAAACTT CAGAAGAGAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGAATCTC 
TCTGATACTT GTGAGATAAA TGTTCATGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGGTCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTA 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGAGA TTTTTCCCGA AGGGGAAAAG TACAAACCCT TAATTACTGG AGAAAAAAAA 
GTACAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGTCCCG AGTTCAGTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAATT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CGTGCACTAT TACTGATGTG TGGATTAGTG AGGAAAAAGA AACAAAGGAA 
ACTCAGTCGG CAGATAGGAT CACCATTCAG GAAAATGAAG TTTCTGAAGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATAGAGCCTG GAACCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCAGAACCAT TTTTCCATAA GGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGACAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCG 
ATGGAACAGT TGCTTAGGGA ACAACCTGGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTGAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCAGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGTGAA 
ATTGCTAGTG AATGTGAATG TGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAACAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ATGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009448565.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009446840.2 Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc84410
Clone ID Related Accession (Same CDS sequence)	XM_009448565.2 , XM_009448565.3
Accession Version	XM_009448565.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009448565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT GGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCAAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ACGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAGCGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCATTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC ACTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAGGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCTGTTA TTTCTGTAAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATACCCGGG AAACTTCAGA AGAGATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG AATCTCTCTG ATACTTGTGA GATAAATGTT 
CATGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG GTCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTAAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGAGATTTT TCCCGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGGAGAA AAAAAAGTAC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GTCCCGAGTT CAGTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAATTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCGTG CACTATTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAACA AAGGAAACTC AGTCGGCAGA TAGGATCACC 
ATTCAGGAAA ATGAAGTTTC TGAAGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATAG AGCCTGGAAC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCAG AACCATTTTT CCATAAGGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGACATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCGATGG AACAGTTGCT TAGGGAACAA 
CCTGGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTGA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCAGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGTGAAATTG CTAGTGAATG TGAATGTGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AACAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTATGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009446840.2
CDS	657..4433
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTCEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEIFPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLDIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009448565.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009448565.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT GGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCAAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ACGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAGCGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCATTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC ACTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAGGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCTGTTA TTTCTGTAAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATACCCGGG AAACTTCAGA AGAGATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG AATCTCTCTG ATACTTGTGA GATAAATGTT 
CATGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG GTCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTAAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGAGATTTT TCCCGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGGAGAA AAAAAAGTAC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GTCCCGAGTT CAGTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAATTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCGTG CACTATTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAACA AAGGAAACTC AGTCGGCAGA TAGGATCACC 
ATTCAGGAAA ATGAAGTTTC TGAAGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATAG AGCCTGGAAC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCAG AACCATTTTT CCATAAGGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGACATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCGATGG AACAGTTGCT TAGGGAACAA 
CCTGGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTGA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCAGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGTGAAATTG CTAGTGAATG TGAATGTGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AACAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTATGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009448565.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009446840.2 Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

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CloneID	OPc84410
Clone ID Related Accession (Same CDS sequence)	XM_009448565.2 , XM_009448565.3
Accession Version	XM_009448565.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009448565.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT GGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCAAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ACGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAGCGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCATTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC ACTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAGGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCTGTTA TTTCTGTAAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATACCCGGG AAACTTCAGA AGAGATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG AATCTCTCTG ATACTTGTGA GATAAATGTT 
CATGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG GTCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTAAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGAGATTTT TCCCGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGGAGAA AAAAAAGTAC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GTCCCGAGTT CAGTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAATTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCGTG CACTATTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAACA AAGGAAACTC AGTCGGCAGA TAGGATCACC 
ATTCAGGAAA ATGAAGTTTC TGAAGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATAG AGCCTGGAAC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCAG AACCATTTTT CCATAAGGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGACATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCGATGG AACAGTTGCT TAGGGAACAA 
CCTGGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTGA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCAGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGTGAAATTG CTAGTGAATG TGAATGTGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AACAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTATGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009446840.2
CDS	657..4433
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTCEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEIFPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLDIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009448565.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009448565.3

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT GGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCAAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ACGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAGCGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCATTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC ACTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAGGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCTGTTA TTTCTGTAAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATACCCGGG AAACTTCAGA AGAGATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG AATCTCTCTG ATACTTGTGA GATAAATGTT 
CATGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG GTCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTAAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGAGATTTT TCCCGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGGAGAA AAAAAAGTAC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GTCCCGAGTT CAGTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAATTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCGTG CACTATTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAACA AAGGAAACTC AGTCGGCAGA TAGGATCACC 
ATTCAGGAAA ATGAAGTTTC TGAAGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATAG AGCCTGGAAC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCAG AACCATTTTT CCATAAGGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGACATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCGATGG AACAGTTGCT TAGGGAACAA 
CCTGGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTGA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCAGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGTGAAATTG CTAGTGAATG TGAATGTGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AACAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTATGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_001153290.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001153290.2 Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPc84411
Clone ID Related Accession (Same CDS sequence)	XM_001153290.4
Accession Version	XM_001153290.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001153290.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT GGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCAAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ACGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GAAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAGCGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCATTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCACTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAGG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTA 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATACCCG GGAAACTTCA 
GAAGAGATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGAATCTCTC TGATACTTGT 
GAGATAAATG TTCATGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGGTCCTAAT 
GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTAAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGAGATT 
TTTCCCGAAG GGGAAAAGTA CAAACCCTTA ATTACTGGAG AAAAAAAAGT ACAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGTCCCGAG 
TTCAGTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAATTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCG 
TGCACTATTA CTGATGTGTG GATTAGTGAG GAAAAAGAAA CAAAGGAAAC TCAGTCGGCA 
GATAGGATCA CCATTCAGGA AAATGAAGTT TCTGAAGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT AGAGCCTGGA ACCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC AGAACCATTT TTCCATAAGG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGACATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCGAT GGAACAGTTG 
CTTAGGGAAC AACCTGGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT GAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCAGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGTGAAAT TGCTAGTGAA 
TGTGAATGTG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAACAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAT GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001153290.2
CDS	581..4309
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTCEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEIFPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLDIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_001153290.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001153290.4

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT GGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCAAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ACGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GAAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAGCGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCATTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCACTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAGG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTA 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATACCCG GGAAACTTCA 
GAAGAGATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGAATCTCTC TGATACTTGT 
GAGATAAATG TTCATGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGGTCCTAAT 
GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTAAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGAGATT 
TTTCCCGAAG GGGAAAAGTA CAAACCCTTA ATTACTGGAG AAAAAAAAGT ACAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGTCCCGAG 
TTCAGTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAATTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCG 
TGCACTATTA CTGATGTGTG GATTAGTGAG GAAAAAGAAA CAAAGGAAAC TCAGTCGGCA 
GATAGGATCA CCATTCAGGA AAATGAAGTT TCTGAAGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT AGAGCCTGGA ACCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC AGAACCATTT TTCCATAAGG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGACATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCGAT GGAACAGTTG 
CTTAGGGAAC AACCTGGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT GAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCAGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGTGAAAT TGCTAGTGAA 
TGTGAATGTG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAACAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAT GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009448566.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009446841.2 Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc84412
Clone ID Related Accession (Same CDS sequence)	XM_009448566.2 , XM_009448566.3
Accession Version	XM_009448566.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006471.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009448566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT GGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCAAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ACGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG AGATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTGTGAGA TAAATGTTCA TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTTTC CCGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA GTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AATTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCGTGCA CTATTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA GGATCACCAT TCAGGAAAAT 
GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATAGAG 
CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCAGAA 
CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG ACATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTGCTTA GGGAACAACC TGGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG AATGTGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAA CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009446841.2
CDS	657..4301
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTCEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEIFPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLDIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009448566.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009448566.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT GGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCAAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ACGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG AGATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTGTGAGA TAAATGTTCA TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTTTC CCGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA GTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AATTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCGTGCA CTATTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA GGATCACCAT TCAGGAAAAT 
GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATAGAG 
CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCAGAA 
CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG ACATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTGCTTA GGGAACAACC TGGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG AATGTGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAA CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_009448566.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009446841.2 Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X10, mRNA.

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CloneID	OPc84412
Clone ID Related Accession (Same CDS sequence)	XM_009448566.2 , XM_009448566.3
Accession Version	XM_009448566.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036883.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009448566.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT GGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCAAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ACGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG AGATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTGTGAGA TAAATGTTCA TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTTTC CCGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA GTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AATTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCGTGCA CTATTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA GGATCACCAT TCAGGAAAAT 
GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATAGAG 
CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCAGAA 
CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG ACATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTGCTTA GGGAACAACC TGGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG AATGTGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAA CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009446841.2
CDS	657..4301
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTCEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEIFPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLDIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_009448566.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes NIMA related kinase 1 (NEK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009448566.3

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT GGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCAAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ACGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTTGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG AGATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTGTGAGA TAAATGTTCA TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTTTC CCGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA GTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AATTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCGTGCA CTATTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA GGATCACCAT TCAGGAAAAT 
GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATAGAG 
CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCAGAA 
CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG ACATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTGCTTA GGGAACAACC TGGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG AATGTGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAA CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NEK1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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