DYSF cDNA ORF clone, Gallus gallus(chicken)

The following DYSF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DYSF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa150250 XM_025149878.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa150251 XM_025149879.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa150238 XM_025149866.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa150239 XM_025149867.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa150240 XM_025149868.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa150241 XM_025149869.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa150242 XM_025149870.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa150243 XM_025149871.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa150244 XM_025149872.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa150245 XM_025149873.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa150246 XM_025149874.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa150247 XM_025149875.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa150248 XM_025149876.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa150249 XM_025149877.1
Latest version!
Gallus gallus dysferlin (DYSF), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa35945 XM_015286070.1
Latest version!
Gallus gallus dysferlin (DYSF), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
5 or more Clone 50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa150250
    Clone ID Related Accession (Same CDS sequence) XM_025149878.1
    Accession Version XM_025149878.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCGTGGGG CCGGGGGGGC CACCTCCGTC CCAGAGTGTC ATCCCGGCGG CGTGTCCCGT 
    GCTGGGAGTG CCACGCTGCA TCGCCCCTCT TCCCCACAGA CCTTCTTCTT CAACGTCTTC
    GAGTCGCCAG CCGAGCTGTT CGACGTGCCC GTCTTCATCA CGGTCCTGGA CTCGCGCTCC
    TTCCGCATGG ATGCGGTGAT CGGAGAGTTT CGGATGGACG TTGAGAGCAT CTATTCGGAG
    CCGAAACACG CGATCCTCAG GCGGTGGCTG CTGCTCTCTG ACCCGGAGGA CTTCACGGCG
    GGGGCAAAGG GGTACCTGAA GGTCAGCCTG CTTGTGCTGG GCCCCGGGGA CGAAGCTCCT
    GTGGAGAAGA AGGACGTCTC TGAAGACAAG GAAGACATTG AGGGGAACCT CCTGCGACCC
    ACGGGGGTCA CCTTGCGAGG GGCTCAGTTC TGCCTGAAGA TCTTCAAGGC TGAGGATTTG
    CCCCAGATGG ACGACGCCGT CATGGACAAC GTCCGGCAGA TCTTCGGCTT CGAAAGCAAC
    AAGAAGAACC TGGTTGACCC CTTTGTGGAA GTCAGCTTTG CGGGGAAGAC GCTCTACTCG
    AGGATCCTGG AGAAGAATGC CAACCCGCAG TGGAATCAGT GCCTGATGCT GCCGGTGATG
    TTCCCTTCCA TGTGCGAGAA GATGAGGATC CGGGTGACCG ACTGGGACCG TCTCACACAC
    AACGACGTCG TCGGCACCGC CTACCTCTGC ATGTCCAAGA TCTCTGCGCC CGGCGGGGAG
    CTGGAGGATG CGTTTCCTGC TCTCACGAAG CCTCTGAAGG CCACAGACCT GGACGACGGG
    CTGGGCTTCC TGCCGACCTT CGGCCCCTGC TACGTCAACC TGTACGGCAG CCCCCGTGAG
    TTCACCGGCT TCCCGGATCC GTACGAGGCG CTCAATTTAG GAAAGGGCGA GGGCGTCGCG
    TACCGCGGCC GGGTGCTGCT GGAGCTGGAG ACCAAGCTGG CGGAGCACGG GGAGCTGAAG
    GTGGAGGACA TCTGTGCGGA TGACATCCTG CGGGTGGAGA AGTACCTGCG GCGGAGGAAG
    TACTCCCTCT TTGCTGCCTT CTACTCGGCC ACCATGCTGC AGGACGTGGA CGATGCCATC
    CAGTTTGAGG TCAGCATCGG CAACTACGGC AACAAGTTCG ACAACACCTG CTTGCCCTTG
    GCCTCCACCA CGCAGTACAG CCGGGCCGTG TTCGATGGTT GCCAGTATTA CTACCTGCCC
    TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCCTCCTATT GGGAAGATAT CAGCTACCGA
    ACCGACACTC AGAACCTCCT CCAGCACGCA GCAGACAGGC TGGAAGCCAA CCTGGAGCGG
    GTCCACCTCG CGCTCAAGGC CAACGGCGCG CCGGGCGAGC TGGAGTCCCT GGCTGCCCAG
    CTGATGGACG ACGTGATTGC AGACTGCAGT GTTCCGCTGC CCGACGTGCT GGGGAAGGCG
    GCCAGCACAC ACCTGGACCA GAACCTGTAC CGCTTCCGCA GCACCAACCT GGAGCAGATG
    GTGCGGGCGG CCCTGAGGCT GAAGCACGAG GAGCTGAGCC TGCCGGCCGT GCTGGAGCAG
    GCGGAGGACT GGCTGTGCCG GCTGCGTGCC ATGGCTGAGG AGCCCCAGAA CAGCCTCCCA
    GACGTGGTGA TCTGGATGCT GCGGGGTGAG AAGCGCGTTG CCTACGCCCG CGTGCCGGCC
    CGCGAGGTGC TCTACTCCCA AAGTGGCCCC AACTGCTGCG GTAGGAACTG TGGCAAGCTG
    CAGACCATCT TCCTGAAGTA CCCCCAGGAG GAGGCGATGG GGCCGCGCAT CCCAGCCCAG
    ATCCGCGTCC AGCTCTGGTT TGGGCTCTCT GTCAATGAGA AGGAGTTCAA CCAATACGCT
    GAGGGGAAGC TCTCCGTCTT TGCTGAAACC TACGAGAACC AGACCAAGCT GGCGCTGGTG
    GGGAACTGGG GCACCACCGG CCTGACTTAC CCCAAATACT CCGATGTCAC CGGCAAGATC
    AAGCTCCCCA AGGACAGCTT CCGGCCCTCC ACGGGCTGGA CCTGGGCTGG TGATTGGTTC
    ATCTGCCCAG AGAAGACGAT GCTGCACGAG GCAGACGCGG GGCACCTGAG CTTCGTGGAG
    GAGGTGTTTG AGAACCAGGT GCGGCTGCCG GGCGGGCAGT GGATCCACAT GTCCGATGCC
    TACACCGATG TGAATGGGGA GAAGGTTCTG CCCAAGGATG AGATCGAGTG TCCTCCGGGC
    TGGAAATGGG AAGACGTGGA GTGGGACACT GACCTCAACA GAGCCGTGGA TGAGAAAGGC
    TGGGAGTACG GCATCACCAT CCCTCCGGAC CGCCGGCCCA AGGCCTGGGT GCCAGCTGAG
    AAGATGTACC ACACCAACCG GCGCCGGCGC TGGGTGCGAC TGCGCAGCAG GGACCTGGCC
    CAGATGGAGA CTGTCAGGAA GCACAAGCAG GACGAGCAGG ACGGCGAGGG CTGGGAATAC
    GCCTCGCTGC TGGGCTGGCG CTTCCACCTG CGGCCGCGTC GCACCGATGC CTTCCGCCGC
    CGCCGTTGGA GACGTAGGAT GGAGCCCTTG GAGCGCACCG GGGCTGCTGC TGTGTTCGCC
    TTGGAGGGGG CCCTGGGAGG AATGACGGAC GACAAAAGCG ACGATGGCAA ATCCGTCTCC
    ACGCTGAGTT TCGGCGTCAA CAGACCCACC ATCTCCTGCA TCTTCGACTA TGGGAACCGC
    TACCACCTGC GCTGCTACAT GTACCAGGCA CGGGACCTGG CTGCCATGGA CAAGGACAGC
    TTCTCGGACC CCTATGCCAT CGTTTCCTTC CTCCACCAAA GCCAGAAGAC GGTGGTGATC
    AAGAACACCC TGAACCCCAC GTGGGACCAG ACGCTCATCT TCTACGAGAT AGAGATCTTC
    GGAGACCCCA AGAACGTGTC TGACTGCCCT CCCAGCATCG TGGTGGAGAT ATACGACCAC
    GACACCTACG GCGCTGATGA GTTCATGGGG CGCTGCGTGT GCCGGCCCAG CCTGCAGCGC
    GCGCCGCGCC TCTCCTGGCA CCCCGTGCTC AAAGGCGGCA GGAACGTCGG CGAGCTGCTG
    GCTGCCTTCG AGCTCATCCA GAGGGAGAAG CCGGCTGTCC ACCACATCCC TGGCTTCGAG
    AACGAGCTGT CCTCCGCTCT GGATGAGCTC TGTGCCCCTG CCTTGTTCTA CGAGGGGCTC
    CTCCGATGGT CTGAGGACTC TGACCTGCCC TACCCACCGC CCCAACGGGA ACCCAACATC
    TTCATGGTCC CCCAAGGGAT CAAACCGGTC CTGCAGCGCA CGGCCATCGA GATCCTGGCC
    TGGGGGCTGA GGAACCTCAA GAGCTACCAG CTGGCCAGCG TCACCTCACC CAGCCTGCTG
    GTGGAGTGCG GGGGGCAGCT CGTGCAGTCC TGTGTCATCA AGAACGTCAA GAAAAACCCC
    AACTTTGATG TCTGTGTGCT CTTCATGGAA GTGCGTCTGC CCAGCGAGAG CCTCTACAGT
    CCCCCCATCA TCATCAAAAT CATCGACAAC CGTCCGTTCG GCCGCAGGCC CGTGGTGGGG
    CAGTGCACCA TCCGCTCACT GGAGGAATTC TACTGTGACC CCTATGGTGA GGAGACGGAT
    GGTGCCCAGG AGCAGGCAGA TGATGTGAGC CTCACGCCCA GGGATGATGT CCTCATTGAC
    ATTGATGACA AAGAGCCTCT CCTTCCCACC CAGCTTGTGG AGGGCGTGAC CAGCTCAGCG
    CTAATTAACC CGCCAGCTTC TAGGTCCGAG CCTCAAGAGG AAGAATTCAT TGACTGGTGG
    AGCAAATTCT ACGCTTCCAC AGGAGAGAGG GAGAAGTGTG GCTCCTACTT GGAGAAGGGC
    TTTGACACCC TGCAGGTCTA TGAGACGGAG CTGGAGCGCG TGGAGGCCTT CGAGCAGCTC
    TCTGACTTCT GCCACACCTT CAGGCTGTAC CGTGGCCGGG TGCAGGATGC CAACGATGAT
    CCCTCAGTTG TGGGCGAGTT CAAGGGCTCC TTCAAAATCT ACCCTCTGCC CGATGACCCA
    CGCGTGCCGC TGCCGCCGCG GCAGTTCCAC CAACTGCCAG CCAGGGGTCC CCAGGAGTGT
    CTCGTCAGGG TCTACATCAT CCGTGCCTTC GGTCTGCAGC CCAAGGATGC CAATGGGAAG
    TGCGATCCGT ATGTGAAGAT TTCAGTGGGA AAGAAATCCA TAAACGACCA AGAAAATTAC
    CTCCCCTCTA CGCTGGAGCC TGTCTTTGGA AAGATGTTTG AGTTGAGCTG CACTCTGCCC
    CTGGAGAAGG ACCTGAAGGT CACACTCTAT GACTACGACC TCCTGTCCAA GGATGAGAAG
    ATTGGGGAGA CCGTCATCGA CCTGGAGAAT CGGTTCCTGT CGAAATATGG GGCGCGCTGT
    GGGCTTCCCC AGACCTACTG CATCTCTGGG CCCAACCAGT GGCGAGACCA GCTCCGTCCC
    TCCCAACTGC TCCACCTCTT CTCCCTGCAG CACCACTACA AGGCTCCCAC CTACACGTCC
    GACCGGCTCA TCTTCCGGGA GCAGGAGTAC ATCCTCTCCG AGCTGGAGGA TGGGAAACCC
    CCCAACCCTC ACCTGGGGCC GGCAGAGGAG CGGCTGGCCC TGCATGCACT GCGGAAACAA
    GGGCTGGTGC CGGAGCACGT GGAGACCCGG CCCCTCTACA GCCCCCTGCA GCCAGAGATC
    GAGCAGGGAA AGCTACAGAT GTGGGTGGAC CTGTTTCCGA AATCCTTGGG TCAGCCTGGC
    CCCCCTTTCA ACATCACACC ACGCAAAGCC AAGAGGTTCT ACTTGCGCTG CATCATCTGG
    AACACCAGGG ACGTCATCCT CGATGACCTC AGCATCACTG GGGAGAAGAT GAGTGACATC
    TATGTCAAAG GCTGGCTGGT TGGCCACGAG GAGAACAAGC AGAAGACAGA CGTGCACTAC
    AGGTCGATGG GAGGAGAAGG CAACTTCAAC TGGAGGTTCA TCTTCCCCTT CGACTATCTC
    CCTGCCGAGC AGATGTGTTG CGTTGCAAAG AAGGAGCATT TCTGGAGCTT GGATAAGACG
    GAAAACAAGA TTTCACCGCA GCTGATCTTC CAGATCTGGG ACAATGACAA GTTCTCCTTC
    GATGACTATT TGGGCTCCAT CCAGATGGAT CTCAACAGGA TGCCCAAGCC TGCCAAGACT
    GCTGAGAAGT GCTCCTTAGA GCTCATAGAT GACAGCTTGT CTGCAAGCCG CTTTGTGTCC
    CTCTTTGAGC AGAAGACTGT CAAGGGCTGG TGGCCCTGCA TTGCAGAGCA GGATGAGAAG
    AAGATCCTGG CAGGAAAACT GGAAATGACT TTGGAAATCG TGGCTGAACA CGAGCATGAA
    GAGCGGCCAG CTGGCGTGGG TCGGGATGAG CCAAACATGA ACCCCAAGCT GGAGGACCCC
    AAGCGCCCAG AGACCTCCTT CCTGTGGTTC ACCTCCCCGT ACAAGACCCT GAAGTACATC
    CTGTGGCGCC GGTACAAGTG GCTGCTCATC CTGGCCATCG TGCTCTTTGT TTTGCTGCTC
    TTCCTTGGGA TCTTCATCTA CGCCTTCCCG AACTACGCTG CTATGAAGCT GGTGAAACCT
    TTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005646.1
    CDS155..5803
    Translation

    Target ORF information:

    RefSeq Version XM_025149878.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149878.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCGTGGGG CCGGGGGGGC CACCTCCGTC CCAGAGTGTC ATCCCGGCGG CGTGTCCCGT 
    GCTGGGAGTG CCACGCTGCA TCGCCCCTCT TCCCCACAGA CCTTCTTCTT CAACGTCTTC
    GAGTCGCCAG CCGAGCTGTT CGACGTGCCC GTCTTCATCA CGGTCCTGGA CTCGCGCTCC
    TTCCGCATGG ATGCGGTGAT CGGAGAGTTT CGGATGGACG TTGAGAGCAT CTATTCGGAG
    CCGAAACACG CGATCCTCAG GCGGTGGCTG CTGCTCTCTG ACCCGGAGGA CTTCACGGCG
    GGGGCAAAGG GGTACCTGAA GGTCAGCCTG CTTGTGCTGG GCCCCGGGGA CGAAGCTCCT
    GTGGAGAAGA AGGACGTCTC TGAAGACAAG GAAGACATTG AGGGGAACCT CCTGCGACCC
    ACGGGGGTCA CCTTGCGAGG GGCTCAGTTC TGCCTGAAGA TCTTCAAGGC TGAGGATTTG
    CCCCAGATGG ACGACGCCGT CATGGACAAC GTCCGGCAGA TCTTCGGCTT CGAAAGCAAC
    AAGAAGAACC TGGTTGACCC CTTTGTGGAA GTCAGCTTTG CGGGGAAGAC GCTCTACTCG
    AGGATCCTGG AGAAGAATGC CAACCCGCAG TGGAATCAGT GCCTGATGCT GCCGGTGATG
    TTCCCTTCCA TGTGCGAGAA GATGAGGATC CGGGTGACCG ACTGGGACCG TCTCACACAC
    AACGACGTCG TCGGCACCGC CTACCTCTGC ATGTCCAAGA TCTCTGCGCC CGGCGGGGAG
    CTGGAGGATG CGTTTCCTGC TCTCACGAAG CCTCTGAAGG CCACAGACCT GGACGACGGG
    CTGGGCTTCC TGCCGACCTT CGGCCCCTGC TACGTCAACC TGTACGGCAG CCCCCGTGAG
    TTCACCGGCT TCCCGGATCC GTACGAGGCG CTCAATTTAG GAAAGGGCGA GGGCGTCGCG
    TACCGCGGCC GGGTGCTGCT GGAGCTGGAG ACCAAGCTGG CGGAGCACGG GGAGCTGAAG
    GTGGAGGACA TCTGTGCGGA TGACATCCTG CGGGTGGAGA AGTACCTGCG GCGGAGGAAG
    TACTCCCTCT TTGCTGCCTT CTACTCGGCC ACCATGCTGC AGGACGTGGA CGATGCCATC
    CAGTTTGAGG TCAGCATCGG CAACTACGGC AACAAGTTCG ACAACACCTG CTTGCCCTTG
    GCCTCCACCA CGCAGTACAG CCGGGCCGTG TTCGATGGTT GCCAGTATTA CTACCTGCCC
    TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCCTCCTATT GGGAAGATAT CAGCTACCGA
    ACCGACACTC AGAACCTCCT CCAGCACGCA GCAGACAGGC TGGAAGCCAA CCTGGAGCGG
    GTCCACCTCG CGCTCAAGGC CAACGGCGCG CCGGGCGAGC TGGAGTCCCT GGCTGCCCAG
    CTGATGGACG ACGTGATTGC AGACTGCAGT GTTCCGCTGC CCGACGTGCT GGGGAAGGCG
    GCCAGCACAC ACCTGGACCA GAACCTGTAC CGCTTCCGCA GCACCAACCT GGAGCAGATG
    GTGCGGGCGG CCCTGAGGCT GAAGCACGAG GAGCTGAGCC TGCCGGCCGT GCTGGAGCAG
    GCGGAGGACT GGCTGTGCCG GCTGCGTGCC ATGGCTGAGG AGCCCCAGAA CAGCCTCCCA
    GACGTGGTGA TCTGGATGCT GCGGGGTGAG AAGCGCGTTG CCTACGCCCG CGTGCCGGCC
    CGCGAGGTGC TCTACTCCCA AAGTGGCCCC AACTGCTGCG GTAGGAACTG TGGCAAGCTG
    CAGACCATCT TCCTGAAGTA CCCCCAGGAG GAGGCGATGG GGCCGCGCAT CCCAGCCCAG
    ATCCGCGTCC AGCTCTGGTT TGGGCTCTCT GTCAATGAGA AGGAGTTCAA CCAATACGCT
    GAGGGGAAGC TCTCCGTCTT TGCTGAAACC TACGAGAACC AGACCAAGCT GGCGCTGGTG
    GGGAACTGGG GCACCACCGG CCTGACTTAC CCCAAATACT CCGATGTCAC CGGCAAGATC
    AAGCTCCCCA AGGACAGCTT CCGGCCCTCC ACGGGCTGGA CCTGGGCTGG TGATTGGTTC
    ATCTGCCCAG AGAAGACGAT GCTGCACGAG GCAGACGCGG GGCACCTGAG CTTCGTGGAG
    GAGGTGTTTG AGAACCAGGT GCGGCTGCCG GGCGGGCAGT GGATCCACAT GTCCGATGCC
    TACACCGATG TGAATGGGGA GAAGGTTCTG CCCAAGGATG AGATCGAGTG TCCTCCGGGC
    TGGAAATGGG AAGACGTGGA GTGGGACACT GACCTCAACA GAGCCGTGGA TGAGAAAGGC
    TGGGAGTACG GCATCACCAT CCCTCCGGAC CGCCGGCCCA AGGCCTGGGT GCCAGCTGAG
    AAGATGTACC ACACCAACCG GCGCCGGCGC TGGGTGCGAC TGCGCAGCAG GGACCTGGCC
    CAGATGGAGA CTGTCAGGAA GCACAAGCAG GACGAGCAGG ACGGCGAGGG CTGGGAATAC
    GCCTCGCTGC TGGGCTGGCG CTTCCACCTG CGGCCGCGTC GCACCGATGC CTTCCGCCGC
    CGCCGTTGGA GACGTAGGAT GGAGCCCTTG GAGCGCACCG GGGCTGCTGC TGTGTTCGCC
    TTGGAGGGGG CCCTGGGAGG AATGACGGAC GACAAAAGCG ACGATGGCAA ATCCGTCTCC
    ACGCTGAGTT TCGGCGTCAA CAGACCCACC ATCTCCTGCA TCTTCGACTA TGGGAACCGC
    TACCACCTGC GCTGCTACAT GTACCAGGCA CGGGACCTGG CTGCCATGGA CAAGGACAGC
    TTCTCGGACC CCTATGCCAT CGTTTCCTTC CTCCACCAAA GCCAGAAGAC GGTGGTGATC
    AAGAACACCC TGAACCCCAC GTGGGACCAG ACGCTCATCT TCTACGAGAT AGAGATCTTC
    GGAGACCCCA AGAACGTGTC TGACTGCCCT CCCAGCATCG TGGTGGAGAT ATACGACCAC
    GACACCTACG GCGCTGATGA GTTCATGGGG CGCTGCGTGT GCCGGCCCAG CCTGCAGCGC
    GCGCCGCGCC TCTCCTGGCA CCCCGTGCTC AAAGGCGGCA GGAACGTCGG CGAGCTGCTG
    GCTGCCTTCG AGCTCATCCA GAGGGAGAAG CCGGCTGTCC ACCACATCCC TGGCTTCGAG
    AACGAGCTGT CCTCCGCTCT GGATGAGCTC TGTGCCCCTG CCTTGTTCTA CGAGGGGCTC
    CTCCGATGGT CTGAGGACTC TGACCTGCCC TACCCACCGC CCCAACGGGA ACCCAACATC
    TTCATGGTCC CCCAAGGGAT CAAACCGGTC CTGCAGCGCA CGGCCATCGA GATCCTGGCC
    TGGGGGCTGA GGAACCTCAA GAGCTACCAG CTGGCCAGCG TCACCTCACC CAGCCTGCTG
    GTGGAGTGCG GGGGGCAGCT CGTGCAGTCC TGTGTCATCA AGAACGTCAA GAAAAACCCC
    AACTTTGATG TCTGTGTGCT CTTCATGGAA GTGCGTCTGC CCAGCGAGAG CCTCTACAGT
    CCCCCCATCA TCATCAAAAT CATCGACAAC CGTCCGTTCG GCCGCAGGCC CGTGGTGGGG
    CAGTGCACCA TCCGCTCACT GGAGGAATTC TACTGTGACC CCTATGGTGA GGAGACGGAT
    GGTGCCCAGG AGCAGGCAGA TGATGTGAGC CTCACGCCCA GGGATGATGT CCTCATTGAC
    ATTGATGACA AAGAGCCTCT CCTTCCCACC CAGCTTGTGG AGGGCGTGAC CAGCTCAGCG
    CTAATTAACC CGCCAGCTTC TAGGTCCGAG CCTCAAGAGG AAGAATTCAT TGACTGGTGG
    AGCAAATTCT ACGCTTCCAC AGGAGAGAGG GAGAAGTGTG GCTCCTACTT GGAGAAGGGC
    TTTGACACCC TGCAGGTCTA TGAGACGGAG CTGGAGCGCG TGGAGGCCTT CGAGCAGCTC
    TCTGACTTCT GCCACACCTT CAGGCTGTAC CGTGGCCGGG TGCAGGATGC CAACGATGAT
    CCCTCAGTTG TGGGCGAGTT CAAGGGCTCC TTCAAAATCT ACCCTCTGCC CGATGACCCA
    CGCGTGCCGC TGCCGCCGCG GCAGTTCCAC CAACTGCCAG CCAGGGGTCC CCAGGAGTGT
    CTCGTCAGGG TCTACATCAT CCGTGCCTTC GGTCTGCAGC CCAAGGATGC CAATGGGAAG
    TGCGATCCGT ATGTGAAGAT TTCAGTGGGA AAGAAATCCA TAAACGACCA AGAAAATTAC
    CTCCCCTCTA CGCTGGAGCC TGTCTTTGGA AAGATGTTTG AGTTGAGCTG CACTCTGCCC
    CTGGAGAAGG ACCTGAAGGT CACACTCTAT GACTACGACC TCCTGTCCAA GGATGAGAAG
    ATTGGGGAGA CCGTCATCGA CCTGGAGAAT CGGTTCCTGT CGAAATATGG GGCGCGCTGT
    GGGCTTCCCC AGACCTACTG CATCTCTGGG CCCAACCAGT GGCGAGACCA GCTCCGTCCC
    TCCCAACTGC TCCACCTCTT CTCCCTGCAG CACCACTACA AGGCTCCCAC CTACACGTCC
    GACCGGCTCA TCTTCCGGGA GCAGGAGTAC ATCCTCTCCG AGCTGGAGGA TGGGAAACCC
    CCCAACCCTC ACCTGGGGCC GGCAGAGGAG CGGCTGGCCC TGCATGCACT GCGGAAACAA
    GGGCTGGTGC CGGAGCACGT GGAGACCCGG CCCCTCTACA GCCCCCTGCA GCCAGAGATC
    GAGCAGGGAA AGCTACAGAT GTGGGTGGAC CTGTTTCCGA AATCCTTGGG TCAGCCTGGC
    CCCCCTTTCA ACATCACACC ACGCAAAGCC AAGAGGTTCT ACTTGCGCTG CATCATCTGG
    AACACCAGGG ACGTCATCCT CGATGACCTC AGCATCACTG GGGAGAAGAT GAGTGACATC
    TATGTCAAAG GCTGGCTGGT TGGCCACGAG GAGAACAAGC AGAAGACAGA CGTGCACTAC
    AGGTCGATGG GAGGAGAAGG CAACTTCAAC TGGAGGTTCA TCTTCCCCTT CGACTATCTC
    CCTGCCGAGC AGATGTGTTG CGTTGCAAAG AAGGAGCATT TCTGGAGCTT GGATAAGACG
    GAAAACAAGA TTTCACCGCA GCTGATCTTC CAGATCTGGG ACAATGACAA GTTCTCCTTC
    GATGACTATT TGGGCTCCAT CCAGATGGAT CTCAACAGGA TGCCCAAGCC TGCCAAGACT
    GCTGAGAAGT GCTCCTTAGA GCTCATAGAT GACAGCTTGT CTGCAAGCCG CTTTGTGTCC
    CTCTTTGAGC AGAAGACTGT CAAGGGCTGG TGGCCCTGCA TTGCAGAGCA GGATGAGAAG
    AAGATCCTGG CAGGAAAACT GGAAATGACT TTGGAAATCG TGGCTGAACA CGAGCATGAA
    GAGCGGCCAG CTGGCGTGGG TCGGGATGAG CCAAACATGA ACCCCAAGCT GGAGGACCCC
    AAGCGCCCAG AGACCTCCTT CCTGTGGTTC ACCTCCCCGT ACAAGACCCT GAAGTACATC
    CTGTGGCGCC GGTACAAGTG GCTGCTCATC CTGGCCATCG TGCTCTTTGT TTTGCTGCTC
    TTCCTTGGGA TCTTCATCTA CGCCTTCCCG AACTACGCTG CTATGAAGCT GGTGAAACCT
    TTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150251
    Clone ID Related Accession (Same CDS sequence) XM_025149879.1
    Accession Version XM_025149879.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGATGC GTTTCCTGCT CTCACGAAGC CTCTGAAGGC CACAGACCTG
    GACGACGGGC TGGGCTTCCT GCCGACCTTC GGCCCCTGCT ACGTCAACCT GTACGGCAGC
    CCCCGTGAGT TCACCGGCTT CCCGGATCCG TACGAGGCGC TCAATTTAGG AAAGGGCGAG
    GGCGTCGCGT ACCGCGGCCG GGTGCTGCTG GAGCTGGAGA CCAAGCTGGC GGAGCACGGG
    GAGCTGAAGG TGGAGGACAT CTGTGCGGAT GACATCCTGC GGGTGGAGAA GTACCTGCGG
    CGGAGGAAGT ACTCCCTCTT TGCTGCCTTC TACTCGGCCA CCATGCTGCA GGACGTGGAC
    GATGCCATCC AGTTTGAGGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CAACACCTGC
    TTGCCCTTGG CCTCCACCAC GCAGTACAGC CGGGCCGTGT TCGATGGTTG CCAGTATTAC
    TACCTGCCCT GGGGCAACGT GAAGCCCGTG GTGGTGCTGT CCTCCTATTG GGAAGATATC
    AGCTACCGAA CCGACACTCA GAACCTCCTC CAGCACGCAG CAGACAGGCT GGAAGCCAAC
    CTGGAGCGGG TCCACCTCGC GCTCAAGGCC AACGGCGCGC CGGGCGAGCT GGAGTCCCTG
    GCTGCCCAGC TGATGGACGA CGTGATTGCA GACTGCAGTG TTCCGCTGCC CGACGTGCTG
    GGGAAGGCGG CCAGCACACA CCTGGACCAG AACCTGTACC GCTTCCGCAG CACCAACCTG
    GAGCAGATGG TGCGGGCGGC CCTGAGGCTG AAGCACGAGG AGCTGAGCCT GCCGGCCGTG
    CTGGAGCAGG CGGAGGACTG GCTGTGCCGG CTGCGTGCCA TGGCTGAGGA GCCCCAGAAC
    AGCCTCCCAG ACGTGGTGAT CTGGATGCTG CGGGGTGAGA AGCGCGTTGC CTACGCCCGC
    GTGCCGGCCC GCGAGGTGCT CTACTCCCAA AGTGGCCCCA ACTGCTGCGG TAGGAACTGT
    GGCAAGCTGC AGACCATCTT CCTGAAGTAC CCCCAGGAGG AGGCGATGGG GCCGCGCATC
    CCAGCCCAGA TCCGCGTCCA GCTCTGGTTT GGGCTCTCTG TCAATGAGAA GGAGTTCAAC
    CAATACGCTG AGGGGAAGCT CTCCGTCTTT GCTGAAACCT ACGAGAACCA GACCAAGCTG
    GCGCTGGTGG GGAACTGGGG CACCACCGGC CTGACTTACC CCAAATACTC CGATGTCACC
    GGCAAGATCA AGCTCCCCAA GGACAGCTTC CGGCCCTCCA CGGGCTGGAC CTGGGCTGGT
    GATTGGTTCA TCTGCCCAGA GAAGACGATG CTGCACGAGG CAGACGCGGG GCACCTGAGC
    TTCGTGGAGG AGGTGTTTGA GAACCAGGTG CGGCTGCCGG GCGGGCAGTG GATCCACATG
    TCCGATGCCT ACACCGATGT GAATGGGGAG AAGGTTCTGC CCAAGGATGA GATCGAGTGT
    CCTCCGGGCT GGAAATGGGA AGACGTGGAG TGGGACACTG ACCTCAACAG AGCCGTGGAT
    GAGAAAGGCT GGGAGTACGG CATCACCATC CCTCCGGACC GCCGGCCCAA GGCCTGGGTG
    CCAGCTGAGA AGATGTACCA CACCAACCGG CGCCGGCGCT GGGTGCGACT GCGCAGCAGG
    GACCTGGCCC AGATGGAGAC TGTCAGGAAG CACAAGCAGG ACGAGCAGGA CGGCGAGGGC
    TGGGAATACG CCTCGCTGCT GGGCTGGCGC TTCCACCTGC GGCCGCGTCG CACCGATGCC
    TTCCGCCGCC GCCGTTGGAG ACGTAGGATG GAGCCCTTGG AGCGCACCGG GGCTGCTGCT
    GTGTTCGCCT TGGAGGGGGC CCTGGGAGGA ATGACGGACG ACAAAAGCGA CGATGGCAAA
    TCCGTCTCCA CGCTGAGTTT CGGCGTCAAC AGACCCACCA TCTCCTGCAT CTTCGACTAT
    GGGAACCGCT ACCACCTGCG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCCATGGAC
    AAGGACAGCT TCTCGGACCC CTATGCCATC GTTTCCTTCC TCCACCAAAG CCAGAAGACG
    GTGGTGATCA AGAACACCCT GAACCCCACG TGGGACCAGA CGCTCATCTT CTACGAGATA
    GAGATCTTCG GAGACCCCAA GAACGTGTCT GACTGCCCTC CCAGCATCGT GGTGGAGATA
    TACGACCACG ACACCTACGG CGCTGATGAG TTCATGGGGC GCTGCGTGTG CCGGCCCAGC
    CTGCAGCGCG CGCCGCGCCT CTCCTGGCAC CCCGTGCTCA AAGGCGGCAG GAACGTCGGC
    GAGCTGCTGG CTGCCTTCGA GCTCATCCAG AGGGAGAAGC CGGCTGTCCA CCACATCCCT
    GGCTTCGAGA ACGAGCTGTC CTCCGCTCTG GATGAGCTCT GTGCCCCTGC CTTGTTCTAC
    GAGGGGCTCC TCCGATGGTC TGAGGACTCT GACCTGCCCT ACCCACCGCC CCAACGGGAA
    CCCAACATCT TCATGGTCCC CCAAGGGATC AAACCGGTCC TGCAGCGCAC GGCCATCGAG
    ATCCTGGCCT GGGGGCTGAG GAACCTCAAG AGCTACCAGC TGGCCAGCGT CACCTCACCC
    AGCCTGCTGG TGGAGTGCGG GGGGCAGCTC GTGCAGTCCT GTGTCATCAA GAACGTCAAG
    AAAAACCCCA ACTTTGATGT CTGTGTGCTC TTCATGGAAG TGCGTCTGCC CAGCGAGAGC
    CTCTACAGTC CCCCCATCAT CATCAAAATC ATCGACAACC GTCCGTTCGG CCGCAGGCCC
    GTGGTGGGGC AGTGCACCAT CCGCTCACTG GAGGAATTCT ACTGTGACCC CTATGGTGAG
    GAGACGGATG GTGCCCAGGA GCAGGCAGAT GATGTGAGCC TCACGCCCAG GGATGATGTC
    CTCATTGACA TTGATGACAA AGAGCCTCTC CTTCCCACCC AGCTTGTGGA GGGCGTGACC
    AGCTCAGCGC TAATTAACCC GCCAGCTTCT AGGTCCGAGC CTCAAGAGGA AGAATTCATT
    GACTGGTGGA GCAAATTCTA CGCTTCCACA GGAGAGAGGG AGAAGTGTGG CTCCTACTTG
    GAGAAGGGCT TTGACACCCT GCAGGTCTAT GAGACGGAGC TGGAGCGCGT GGAGGCCTTC
    GAGCAGCTCT CTGACTTCTG CCACACCTTC AGGCTGTACC GTGGCCGGGT GCAGGATGCC
    AACGATGATC CCTCAGTTGT GGGCGAGTTC AAGGGCTCCT TCAAAATCTA CCCTCTGCCC
    GATGACCCAC GCGTGCCGCT GCCGCCGCGG CAGTTCCACC AACTGCCAGC CAGGGGTCCC
    CAGGAGTGTC TCGTCAGGGT CTACATCATC CGTGCCTTCG GTCTGCAGCC CAAGGATGCC
    AATGGGAAGT GCGATCCGTA TGTGAAGATT TCAGTGGGAA AGAAATCCAT AAACGACCAA
    GAAAATTACC TCCCCTCTAC GCTGGAGCCT GTCTTTGGAA AGATGTTTGA GTTGAGCTGC
    ACTCTGCCCC TGGAGAAGGA CCTGAAGGTC ACACTCTATG ACTACGACCT CCTGTCCAAG
    GATGAGAAGA TTGGGGAGAC CGTCATCGAC CTGGAGAATC GGTTCCTGTC GAAATATGGG
    GCGCGCTGTG GGCTTCCCCA GACCTACTGC ATCTCTGGGC CCAACCAGTG GCGAGACCAG
    CTCCGTCCCT CCCAACTGCT CCACCTCTTC TCCCTGCAGC ACCACTACAA GGCTCCCACC
    TACACGTCCG ACCGGCTCAT CTTCCGGGAG CAGGAGTACA TCCTCTCCGA GCTGGCCCAT
    GTGATTTGGG GCTGGGGTCG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005647.1
    CDS652..6135
    Translation

    Target ORF information:

    RefSeq Version XM_025149879.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGATGC GTTTCCTGCT CTCACGAAGC CTCTGAAGGC CACAGACCTG
    GACGACGGGC TGGGCTTCCT GCCGACCTTC GGCCCCTGCT ACGTCAACCT GTACGGCAGC
    CCCCGTGAGT TCACCGGCTT CCCGGATCCG TACGAGGCGC TCAATTTAGG AAAGGGCGAG
    GGCGTCGCGT ACCGCGGCCG GGTGCTGCTG GAGCTGGAGA CCAAGCTGGC GGAGCACGGG
    GAGCTGAAGG TGGAGGACAT CTGTGCGGAT GACATCCTGC GGGTGGAGAA GTACCTGCGG
    CGGAGGAAGT ACTCCCTCTT TGCTGCCTTC TACTCGGCCA CCATGCTGCA GGACGTGGAC
    GATGCCATCC AGTTTGAGGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CAACACCTGC
    TTGCCCTTGG CCTCCACCAC GCAGTACAGC CGGGCCGTGT TCGATGGTTG CCAGTATTAC
    TACCTGCCCT GGGGCAACGT GAAGCCCGTG GTGGTGCTGT CCTCCTATTG GGAAGATATC
    AGCTACCGAA CCGACACTCA GAACCTCCTC CAGCACGCAG CAGACAGGCT GGAAGCCAAC
    CTGGAGCGGG TCCACCTCGC GCTCAAGGCC AACGGCGCGC CGGGCGAGCT GGAGTCCCTG
    GCTGCCCAGC TGATGGACGA CGTGATTGCA GACTGCAGTG TTCCGCTGCC CGACGTGCTG
    GGGAAGGCGG CCAGCACACA CCTGGACCAG AACCTGTACC GCTTCCGCAG CACCAACCTG
    GAGCAGATGG TGCGGGCGGC CCTGAGGCTG AAGCACGAGG AGCTGAGCCT GCCGGCCGTG
    CTGGAGCAGG CGGAGGACTG GCTGTGCCGG CTGCGTGCCA TGGCTGAGGA GCCCCAGAAC
    AGCCTCCCAG ACGTGGTGAT CTGGATGCTG CGGGGTGAGA AGCGCGTTGC CTACGCCCGC
    GTGCCGGCCC GCGAGGTGCT CTACTCCCAA AGTGGCCCCA ACTGCTGCGG TAGGAACTGT
    GGCAAGCTGC AGACCATCTT CCTGAAGTAC CCCCAGGAGG AGGCGATGGG GCCGCGCATC
    CCAGCCCAGA TCCGCGTCCA GCTCTGGTTT GGGCTCTCTG TCAATGAGAA GGAGTTCAAC
    CAATACGCTG AGGGGAAGCT CTCCGTCTTT GCTGAAACCT ACGAGAACCA GACCAAGCTG
    GCGCTGGTGG GGAACTGGGG CACCACCGGC CTGACTTACC CCAAATACTC CGATGTCACC
    GGCAAGATCA AGCTCCCCAA GGACAGCTTC CGGCCCTCCA CGGGCTGGAC CTGGGCTGGT
    GATTGGTTCA TCTGCCCAGA GAAGACGATG CTGCACGAGG CAGACGCGGG GCACCTGAGC
    TTCGTGGAGG AGGTGTTTGA GAACCAGGTG CGGCTGCCGG GCGGGCAGTG GATCCACATG
    TCCGATGCCT ACACCGATGT GAATGGGGAG AAGGTTCTGC CCAAGGATGA GATCGAGTGT
    CCTCCGGGCT GGAAATGGGA AGACGTGGAG TGGGACACTG ACCTCAACAG AGCCGTGGAT
    GAGAAAGGCT GGGAGTACGG CATCACCATC CCTCCGGACC GCCGGCCCAA GGCCTGGGTG
    CCAGCTGAGA AGATGTACCA CACCAACCGG CGCCGGCGCT GGGTGCGACT GCGCAGCAGG
    GACCTGGCCC AGATGGAGAC TGTCAGGAAG CACAAGCAGG ACGAGCAGGA CGGCGAGGGC
    TGGGAATACG CCTCGCTGCT GGGCTGGCGC TTCCACCTGC GGCCGCGTCG CACCGATGCC
    TTCCGCCGCC GCCGTTGGAG ACGTAGGATG GAGCCCTTGG AGCGCACCGG GGCTGCTGCT
    GTGTTCGCCT TGGAGGGGGC CCTGGGAGGA ATGACGGACG ACAAAAGCGA CGATGGCAAA
    TCCGTCTCCA CGCTGAGTTT CGGCGTCAAC AGACCCACCA TCTCCTGCAT CTTCGACTAT
    GGGAACCGCT ACCACCTGCG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCCATGGAC
    AAGGACAGCT TCTCGGACCC CTATGCCATC GTTTCCTTCC TCCACCAAAG CCAGAAGACG
    GTGGTGATCA AGAACACCCT GAACCCCACG TGGGACCAGA CGCTCATCTT CTACGAGATA
    GAGATCTTCG GAGACCCCAA GAACGTGTCT GACTGCCCTC CCAGCATCGT GGTGGAGATA
    TACGACCACG ACACCTACGG CGCTGATGAG TTCATGGGGC GCTGCGTGTG CCGGCCCAGC
    CTGCAGCGCG CGCCGCGCCT CTCCTGGCAC CCCGTGCTCA AAGGCGGCAG GAACGTCGGC
    GAGCTGCTGG CTGCCTTCGA GCTCATCCAG AGGGAGAAGC CGGCTGTCCA CCACATCCCT
    GGCTTCGAGA ACGAGCTGTC CTCCGCTCTG GATGAGCTCT GTGCCCCTGC CTTGTTCTAC
    GAGGGGCTCC TCCGATGGTC TGAGGACTCT GACCTGCCCT ACCCACCGCC CCAACGGGAA
    CCCAACATCT TCATGGTCCC CCAAGGGATC AAACCGGTCC TGCAGCGCAC GGCCATCGAG
    ATCCTGGCCT GGGGGCTGAG GAACCTCAAG AGCTACCAGC TGGCCAGCGT CACCTCACCC
    AGCCTGCTGG TGGAGTGCGG GGGGCAGCTC GTGCAGTCCT GTGTCATCAA GAACGTCAAG
    AAAAACCCCA ACTTTGATGT CTGTGTGCTC TTCATGGAAG TGCGTCTGCC CAGCGAGAGC
    CTCTACAGTC CCCCCATCAT CATCAAAATC ATCGACAACC GTCCGTTCGG CCGCAGGCCC
    GTGGTGGGGC AGTGCACCAT CCGCTCACTG GAGGAATTCT ACTGTGACCC CTATGGTGAG
    GAGACGGATG GTGCCCAGGA GCAGGCAGAT GATGTGAGCC TCACGCCCAG GGATGATGTC
    CTCATTGACA TTGATGACAA AGAGCCTCTC CTTCCCACCC AGCTTGTGGA GGGCGTGACC
    AGCTCAGCGC TAATTAACCC GCCAGCTTCT AGGTCCGAGC CTCAAGAGGA AGAATTCATT
    GACTGGTGGA GCAAATTCTA CGCTTCCACA GGAGAGAGGG AGAAGTGTGG CTCCTACTTG
    GAGAAGGGCT TTGACACCCT GCAGGTCTAT GAGACGGAGC TGGAGCGCGT GGAGGCCTTC
    GAGCAGCTCT CTGACTTCTG CCACACCTTC AGGCTGTACC GTGGCCGGGT GCAGGATGCC
    AACGATGATC CCTCAGTTGT GGGCGAGTTC AAGGGCTCCT TCAAAATCTA CCCTCTGCCC
    GATGACCCAC GCGTGCCGCT GCCGCCGCGG CAGTTCCACC AACTGCCAGC CAGGGGTCCC
    CAGGAGTGTC TCGTCAGGGT CTACATCATC CGTGCCTTCG GTCTGCAGCC CAAGGATGCC
    AATGGGAAGT GCGATCCGTA TGTGAAGATT TCAGTGGGAA AGAAATCCAT AAACGACCAA
    GAAAATTACC TCCCCTCTAC GCTGGAGCCT GTCTTTGGAA AGATGTTTGA GTTGAGCTGC
    ACTCTGCCCC TGGAGAAGGA CCTGAAGGTC ACACTCTATG ACTACGACCT CCTGTCCAAG
    GATGAGAAGA TTGGGGAGAC CGTCATCGAC CTGGAGAATC GGTTCCTGTC GAAATATGGG
    GCGCGCTGTG GGCTTCCCCA GACCTACTGC ATCTCTGGGC CCAACCAGTG GCGAGACCAG
    CTCCGTCCCT CCCAACTGCT CCACCTCTTC TCCCTGCAGC ACCACTACAA GGCTCCCACC
    TACACGTCCG ACCGGCTCAT CTTCCGGGAG CAGGAGTACA TCCTCTCCGA GCTGGCCCAT
    GTGATTTGGG GCTGGGGTCG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150238
    Clone ID Related Accession (Same CDS sequence) XM_025149866.1
    Accession Version XM_025149866.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGATGC GTTTCCTGCT CTCACGAAGC CTCTGAAGGC CACAGACCTG
    GACGACGGGC TGGGCTTCCT GCCGACCTTC GGCCCCTGCT ACGTCAACCT GTACGGCAGC
    CCCCGTGAGT TCACCGGCTT CCCGGATCCG TACGAGGCGC TCAATTTAGG AAAGGGCGAG
    GGCGTCGCGT ACCGCGGCCG GGTGCTGCTG GAGCTGGAGA CCAAGCTGGC GGAGCACGGG
    GAGCTGAAGG TGGAGGACAT CTGTGCGGAT GACATCCTGC GGGTGGAGAA GTACCTGCGG
    CGGAGGAAGT ACTCCCTCTT TGCTGCCTTC TACTCGGCCA CCATGCTGCA GGACGTGGAC
    GATGCCATCC AGTTTGAGGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CAACACCTGC
    TTGCCCTTGG CCTCCACCAC GCAGTACAGC CGGGCCGTGT TCGATGGTTG CCAGTATTAC
    TACCTGCCCT GGGGCAACGT GAAGCCCGTG GTGGTGCTGT CCTCCTATTG GGAAGATATC
    AGCTACCGAA CCGACACTCA GAACCTCCTC CAGCACGCAG CAGACAGGCT GGAAGCCAAC
    CTGGAGCGGG TCCACCTCGC GCTCAAGGCC AACGGCGCGC CGGGCGAGCT GGAGTCCCTG
    GCTGCCCAGC TGATGGACGA CGTGATTGCA GACTGCAGTG TTCCGCTGCC CGACGTGCTG
    GGGAAGGCGG CCAGCACACA CCTGGACCAG AACCTGTACC GCTTCCGCAG CACCAACCTG
    GAGCAGATGG TGCGGGCGGC CCTGAGGCTG AAGCACGAGG AGCTGAGCCT GCCGGCCGTG
    CTGGAGCAGG CGGAGGACTG GCTGTGCCGG CTGCGTGCCA TGGCTGAGGA GCCCCAGAAC
    AGCCTCCCAG ACGTGGTGAT CTGGATGCTG CGGGGTGAGA AGCGCGTTGC CTACGCCCGC
    GTGCCGGCCC GCGAGGTGCT CTACTCCCAA AGTGGCCCCA ACTGCTGCGG TAGGAACTGT
    GGCAAGCTGC AGACCATCTT CCTGAAGTAC CCCCAGGAGG AGGCGATGGG GCCGCGCATC
    CCAGCCCAGA TCCGCGTCCA GCTCTGGTTT GGGCTCTCTG TCAATGAGAA GGAGTTCAAC
    CAATACGCTG AGGGGAAGCT CTCCGTCTTT GCTGAAACCT ACGAGAACCA GACCAAGCTG
    GCGCTGGTGG GGAACTGGGG CACCACCGGC CTGACTTACC CCAAATACTC CGATGTCACC
    GGCAAGATCA AGCTCCCCAA GGACAGCTTC CGGCCCTCCA CGGGCTGGAC CTGGGCTGGT
    GATTGGTTCA TCTGCCCAGA GAAGACGATG CTGCACGAGG CAGACGCGGG GCACCTGAGC
    TTCGTGGAGG AGGTGTTTGA GAACCAGGTG CGGCTGCCGG GCGGGCAGTG GATCCACATG
    TCCGATGCCT ACACCGATGT GAATGGGGAG AAGGTTCTGC CCAAGGATGA GATCGAGTGT
    CCTCCGGGCT GGAAATGGGA AGACGTGGAG TGGGACACTG ACCTCAACAG AGCCGTGGAT
    GAGAAAGGCT GGGAGTACGG CATCACCATC CCTCCGGACC GCCGGCCCAA GGCCTGGGTG
    CCAGCTGAGA AGATGTACCA CACCAACCGG CGCCGGCGCT GGGTGCGACT GCGCAGCAGG
    GACCTGGCCC AGATGGAGAC TGTCAGGAAG CACAAGCAGG ACGAGCAGGA CGGCGAGGGC
    TGGGAATACG CCTCGCTGCT GGGCTGGCGC TTCCACCTGC GGCCGCGTCG CACCGATGCC
    TTCCGCCGCC GCCGTTGGAG ACGTAGGATG GAGCCCTTGG AGCGCACCGG GGCTGCTGCT
    GTGTTCGCCT TGGAGGGGGC CCTGGGAGGA ATGACGGACG ACAAAAGCGA CGATGGCAAA
    TCCGTCTCCA CGCTGAGTTT CGGCGTCAAC AGACCCACCA TCTCCTGCAT CTTCGACTAT
    GGGAACCGCT ACCACCTGCG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCCATGGAC
    AAGGACAGCT TCTCGGACCC CTATGCCATC GTTTCCTTCC TCCACCAAAG CCAGAAGACG
    GTGGTGATCA AGAACACCCT GAACCCCACG TGGGACCAGA CGCTCATCTT CTACGAGATA
    GAGATCTTCG GAGACCCCAA GAACGTGTCT GACTGCCCTC CCAGCATCGT GGTGGAGATA
    TACGACCACG ACACCTACGG CGCTGATGAG TTCATGGGGC GCTGCGTGTG CCGGCCCAGC
    CTGCAGCGCG CGCCGCGCCT CTCCTGGCAC CCCGTGCTCA AAGGCGGCAG GAACGTCGGC
    GAGCTGCTGG CTGCCTTCGA GCTCATCCAG AGGGAGAAGC CGGCTGTCCA CCACATCCCT
    GGCTTCGAGA ACGAGCTGTC CTCCGCTCTG GATGAGCTCT GTGCCCCTGC CTTGTTCTAC
    GAGGGGCTCC TCCGATGGTC TGAGGACTCT GACCTGCCCT ACCCACCGCC CCAACGGGAA
    CCCAACATCT TCATGGTCCC CCAAGGGATC AAACCGGTCC TGCAGCGCAC GGCCATCGAG
    ATCCTGGCCT GGGGGCTGAG GAACCTCAAG AGCTACCAGC TGGCCAGCGT CACCTCACCC
    AGCCTGCTGG TGGAGTGCGG GGGGCAGCTC GTGCAGTCCT GTGTCATCAA GAACGTCAAG
    AAAAACCCCA ACTTTGATGT CTGTGTGCTC TTCATGGAAG TGCGTCTGCC CAGCGAGAGC
    CTCTACAGTC CCCCCATCAT CATCAAAATC ATCGACAACC GTCCGTTCGG CCGCAGGCCC
    GTGGTGGGGC AGTGCACCAT CCGCTCACTG GAGGAATTCT ACTGTGACCC CTATGGTGAG
    GAGACGGATG GTGCCCAGGA GCAGGCAGAT GATGTGAGCC TCACGCCCAG GGATGATGTC
    CTCATTGACA TTGATGACAA AGAGCCTCTC CTTCCCACCC AGCTTGTGGA GGGCGTGACC
    AGCTCAGCGC TAATTAACCC GCCAGCTTCT AGGTCCGAGC CTCAAGAGGA AGAATTCATT
    GACTGGTGGA GCAAATTCTA CGCTTCCACA GGAGAGAGGG AGAAGTGTGG CTCCTACTTG
    GAGAAGGGCT TTGACACCCT GCAGGTCTAT GAGACGGAGC TGGAGCGCGT GGAGGCCTTC
    GAGCAGCTCT CTGACTTCTG CCACACCTTC AGGCTGTACC GTGGCCGGGT GCAGGATGCC
    AACGATGATC CCTCAGTTGT GGGCGAGTTC AAGGGCTCCT TCAAAATCTA CCCTCTGCCC
    GATGACCCAC GCGTGCCGCT GCCGCCGCGG CAGTTCCACC AACTGCCAGC CAGGGGTCCC
    CAGGAGTGTC TCGTCAGGGT CTACATCATC CGTGCCTTCG GTCTGCAGCC CAAGGATGCC
    AATGGGAAGT GCGATCCGTA TGTGAAGATT TCAGTGGGAA AGAAATCCAT AAACGACCAA
    GAAAATTACC TCCCCTCTAC GCTGGAGCCT GTCTTTGGAA AGATGTTTGA GTTGAGCTGC
    ACTCTGCCCC TGGAGAAGGA CCTGAAGGTC ACACTCTATG ACTACGACCT CCTGTCCAAG
    GATGAGAAGA TTGGGGAGAC CGTCATCGAC CTGGAGAATC GGTTCCTGTC GAAATATGGG
    GCGCGCTGTG GGCTTCCCCA GACCTACTGC ATCTCTGGGC CCAACCAGTG GCGAGACCAG
    CTCCGTCCCT CCCAACTGCT CCACCTCTTC TCCCTGCAGC ACCACTACAA GGCTCCCACC
    TACACGTCCG ACCGGCTCAT CTTCCGGGAG CAGGAGTACA TCCTCTCCGA GCTGGAGGAT
    GGGAAACCCC CCAACCCTCA CCTGGGGCCG GCAGAGGAGC GGCTGGCCCT GCATGCACTG
    CGGAAACAAG GGCTGGTGCC GGAGCACGTG GAGACCCGGC CCCTCTACAG CCCCCTGCAG
    CCAGAGATCG AGCAGGGAAA GCTACAGATG TGGGTGGACC TGTTTCCGAA ATCCTTGGGT
    CAGCCTGGCC CCCCTTTCAA CATCACACCA CGCAAAGCCA AGAGGTTCTA CTTGCGCTGC
    ATCATCTGGA ACACCAGGGA CGTCATCCTC GATGACCTCA GCATCACTGG GGAGAAGATG
    AGTGACATCT ATGTCAAAGG CTGGCTGGTT GGCCACGAGG AGAACAAGCA GAAGACAGAC
    GTGCACTACA GGTCGATGGG AGGAGAAGGC AACTTCAACT GGAGGTTCAT CTTCCCCTTC
    GACTATCTCC CTGCCGAGCA GATGTGTTGC GTTGCAAAGA AGGAGCATTT CTGGAGCTTG
    GATAAGACGG AAAACAAGAT TTCACCGCAG CTGATCTTCC AGATCTGGGA CAATGACAAG
    TTCTCCTTCG ATGACTATTT GGGCTCCATC CAGATGGATC TCAACAGGAT GCCCAAGCCT
    GCCAAGACTG CTGAGAAGTG CTCCTTAGAG CTCATAGATG ACAGCTTGTC TGCAAGCCGC
    TTTGTGTCCC TCTTTGAGCA GAAGACTGTC AAGGGCTGGT GGCCCTGCAT TGCAGAGCAG
    GATGAGAAGA AGATCCTGGC AGGAAAACTG GAAATGACTT TGGAAATCGT GGCTGAACAC
    GAGCATGAAG AGCGGCCAGC TGGCGTGGGT CGGGATGAGC CAAACATGAA CCCCAAGCTG
    GAGGACCCCA AGCGCCCAGA GACCTCCTTC CTGTGGTTCA CCTCCCCGTA CAAGACCCTG
    AAGTACATCC TGTGGCGCCG GTACAAGTGG CTGCTCATCC TGGCCATCGT GCTCTTTGTT
    TTGCTGCTCT TCCTTGGGAT CTTCATCTAC GCCTTCCCGA ACTACGCTGC TATGAAGCTG
    GTGAAACCTT TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005634.1
    CDS648..7145
    Translation

    Target ORF information:

    RefSeq Version XM_025149866.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149866.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGATGC GTTTCCTGCT CTCACGAAGC CTCTGAAGGC CACAGACCTG
    GACGACGGGC TGGGCTTCCT GCCGACCTTC GGCCCCTGCT ACGTCAACCT GTACGGCAGC
    CCCCGTGAGT TCACCGGCTT CCCGGATCCG TACGAGGCGC TCAATTTAGG AAAGGGCGAG
    GGCGTCGCGT ACCGCGGCCG GGTGCTGCTG GAGCTGGAGA CCAAGCTGGC GGAGCACGGG
    GAGCTGAAGG TGGAGGACAT CTGTGCGGAT GACATCCTGC GGGTGGAGAA GTACCTGCGG
    CGGAGGAAGT ACTCCCTCTT TGCTGCCTTC TACTCGGCCA CCATGCTGCA GGACGTGGAC
    GATGCCATCC AGTTTGAGGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CAACACCTGC
    TTGCCCTTGG CCTCCACCAC GCAGTACAGC CGGGCCGTGT TCGATGGTTG CCAGTATTAC
    TACCTGCCCT GGGGCAACGT GAAGCCCGTG GTGGTGCTGT CCTCCTATTG GGAAGATATC
    AGCTACCGAA CCGACACTCA GAACCTCCTC CAGCACGCAG CAGACAGGCT GGAAGCCAAC
    CTGGAGCGGG TCCACCTCGC GCTCAAGGCC AACGGCGCGC CGGGCGAGCT GGAGTCCCTG
    GCTGCCCAGC TGATGGACGA CGTGATTGCA GACTGCAGTG TTCCGCTGCC CGACGTGCTG
    GGGAAGGCGG CCAGCACACA CCTGGACCAG AACCTGTACC GCTTCCGCAG CACCAACCTG
    GAGCAGATGG TGCGGGCGGC CCTGAGGCTG AAGCACGAGG AGCTGAGCCT GCCGGCCGTG
    CTGGAGCAGG CGGAGGACTG GCTGTGCCGG CTGCGTGCCA TGGCTGAGGA GCCCCAGAAC
    AGCCTCCCAG ACGTGGTGAT CTGGATGCTG CGGGGTGAGA AGCGCGTTGC CTACGCCCGC
    GTGCCGGCCC GCGAGGTGCT CTACTCCCAA AGTGGCCCCA ACTGCTGCGG TAGGAACTGT
    GGCAAGCTGC AGACCATCTT CCTGAAGTAC CCCCAGGAGG AGGCGATGGG GCCGCGCATC
    CCAGCCCAGA TCCGCGTCCA GCTCTGGTTT GGGCTCTCTG TCAATGAGAA GGAGTTCAAC
    CAATACGCTG AGGGGAAGCT CTCCGTCTTT GCTGAAACCT ACGAGAACCA GACCAAGCTG
    GCGCTGGTGG GGAACTGGGG CACCACCGGC CTGACTTACC CCAAATACTC CGATGTCACC
    GGCAAGATCA AGCTCCCCAA GGACAGCTTC CGGCCCTCCA CGGGCTGGAC CTGGGCTGGT
    GATTGGTTCA TCTGCCCAGA GAAGACGATG CTGCACGAGG CAGACGCGGG GCACCTGAGC
    TTCGTGGAGG AGGTGTTTGA GAACCAGGTG CGGCTGCCGG GCGGGCAGTG GATCCACATG
    TCCGATGCCT ACACCGATGT GAATGGGGAG AAGGTTCTGC CCAAGGATGA GATCGAGTGT
    CCTCCGGGCT GGAAATGGGA AGACGTGGAG TGGGACACTG ACCTCAACAG AGCCGTGGAT
    GAGAAAGGCT GGGAGTACGG CATCACCATC CCTCCGGACC GCCGGCCCAA GGCCTGGGTG
    CCAGCTGAGA AGATGTACCA CACCAACCGG CGCCGGCGCT GGGTGCGACT GCGCAGCAGG
    GACCTGGCCC AGATGGAGAC TGTCAGGAAG CACAAGCAGG ACGAGCAGGA CGGCGAGGGC
    TGGGAATACG CCTCGCTGCT GGGCTGGCGC TTCCACCTGC GGCCGCGTCG CACCGATGCC
    TTCCGCCGCC GCCGTTGGAG ACGTAGGATG GAGCCCTTGG AGCGCACCGG GGCTGCTGCT
    GTGTTCGCCT TGGAGGGGGC CCTGGGAGGA ATGACGGACG ACAAAAGCGA CGATGGCAAA
    TCCGTCTCCA CGCTGAGTTT CGGCGTCAAC AGACCCACCA TCTCCTGCAT CTTCGACTAT
    GGGAACCGCT ACCACCTGCG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCCATGGAC
    AAGGACAGCT TCTCGGACCC CTATGCCATC GTTTCCTTCC TCCACCAAAG CCAGAAGACG
    GTGGTGATCA AGAACACCCT GAACCCCACG TGGGACCAGA CGCTCATCTT CTACGAGATA
    GAGATCTTCG GAGACCCCAA GAACGTGTCT GACTGCCCTC CCAGCATCGT GGTGGAGATA
    TACGACCACG ACACCTACGG CGCTGATGAG TTCATGGGGC GCTGCGTGTG CCGGCCCAGC
    CTGCAGCGCG CGCCGCGCCT CTCCTGGCAC CCCGTGCTCA AAGGCGGCAG GAACGTCGGC
    GAGCTGCTGG CTGCCTTCGA GCTCATCCAG AGGGAGAAGC CGGCTGTCCA CCACATCCCT
    GGCTTCGAGA ACGAGCTGTC CTCCGCTCTG GATGAGCTCT GTGCCCCTGC CTTGTTCTAC
    GAGGGGCTCC TCCGATGGTC TGAGGACTCT GACCTGCCCT ACCCACCGCC CCAACGGGAA
    CCCAACATCT TCATGGTCCC CCAAGGGATC AAACCGGTCC TGCAGCGCAC GGCCATCGAG
    ATCCTGGCCT GGGGGCTGAG GAACCTCAAG AGCTACCAGC TGGCCAGCGT CACCTCACCC
    AGCCTGCTGG TGGAGTGCGG GGGGCAGCTC GTGCAGTCCT GTGTCATCAA GAACGTCAAG
    AAAAACCCCA ACTTTGATGT CTGTGTGCTC TTCATGGAAG TGCGTCTGCC CAGCGAGAGC
    CTCTACAGTC CCCCCATCAT CATCAAAATC ATCGACAACC GTCCGTTCGG CCGCAGGCCC
    GTGGTGGGGC AGTGCACCAT CCGCTCACTG GAGGAATTCT ACTGTGACCC CTATGGTGAG
    GAGACGGATG GTGCCCAGGA GCAGGCAGAT GATGTGAGCC TCACGCCCAG GGATGATGTC
    CTCATTGACA TTGATGACAA AGAGCCTCTC CTTCCCACCC AGCTTGTGGA GGGCGTGACC
    AGCTCAGCGC TAATTAACCC GCCAGCTTCT AGGTCCGAGC CTCAAGAGGA AGAATTCATT
    GACTGGTGGA GCAAATTCTA CGCTTCCACA GGAGAGAGGG AGAAGTGTGG CTCCTACTTG
    GAGAAGGGCT TTGACACCCT GCAGGTCTAT GAGACGGAGC TGGAGCGCGT GGAGGCCTTC
    GAGCAGCTCT CTGACTTCTG CCACACCTTC AGGCTGTACC GTGGCCGGGT GCAGGATGCC
    AACGATGATC CCTCAGTTGT GGGCGAGTTC AAGGGCTCCT TCAAAATCTA CCCTCTGCCC
    GATGACCCAC GCGTGCCGCT GCCGCCGCGG CAGTTCCACC AACTGCCAGC CAGGGGTCCC
    CAGGAGTGTC TCGTCAGGGT CTACATCATC CGTGCCTTCG GTCTGCAGCC CAAGGATGCC
    AATGGGAAGT GCGATCCGTA TGTGAAGATT TCAGTGGGAA AGAAATCCAT AAACGACCAA
    GAAAATTACC TCCCCTCTAC GCTGGAGCCT GTCTTTGGAA AGATGTTTGA GTTGAGCTGC
    ACTCTGCCCC TGGAGAAGGA CCTGAAGGTC ACACTCTATG ACTACGACCT CCTGTCCAAG
    GATGAGAAGA TTGGGGAGAC CGTCATCGAC CTGGAGAATC GGTTCCTGTC GAAATATGGG
    GCGCGCTGTG GGCTTCCCCA GACCTACTGC ATCTCTGGGC CCAACCAGTG GCGAGACCAG
    CTCCGTCCCT CCCAACTGCT CCACCTCTTC TCCCTGCAGC ACCACTACAA GGCTCCCACC
    TACACGTCCG ACCGGCTCAT CTTCCGGGAG CAGGAGTACA TCCTCTCCGA GCTGGAGGAT
    GGGAAACCCC CCAACCCTCA CCTGGGGCCG GCAGAGGAGC GGCTGGCCCT GCATGCACTG
    CGGAAACAAG GGCTGGTGCC GGAGCACGTG GAGACCCGGC CCCTCTACAG CCCCCTGCAG
    CCAGAGATCG AGCAGGGAAA GCTACAGATG TGGGTGGACC TGTTTCCGAA ATCCTTGGGT
    CAGCCTGGCC CCCCTTTCAA CATCACACCA CGCAAAGCCA AGAGGTTCTA CTTGCGCTGC
    ATCATCTGGA ACACCAGGGA CGTCATCCTC GATGACCTCA GCATCACTGG GGAGAAGATG
    AGTGACATCT ATGTCAAAGG CTGGCTGGTT GGCCACGAGG AGAACAAGCA GAAGACAGAC
    GTGCACTACA GGTCGATGGG AGGAGAAGGC AACTTCAACT GGAGGTTCAT CTTCCCCTTC
    GACTATCTCC CTGCCGAGCA GATGTGTTGC GTTGCAAAGA AGGAGCATTT CTGGAGCTTG
    GATAAGACGG AAAACAAGAT TTCACCGCAG CTGATCTTCC AGATCTGGGA CAATGACAAG
    TTCTCCTTCG ATGACTATTT GGGCTCCATC CAGATGGATC TCAACAGGAT GCCCAAGCCT
    GCCAAGACTG CTGAGAAGTG CTCCTTAGAG CTCATAGATG ACAGCTTGTC TGCAAGCCGC
    TTTGTGTCCC TCTTTGAGCA GAAGACTGTC AAGGGCTGGT GGCCCTGCAT TGCAGAGCAG
    GATGAGAAGA AGATCCTGGC AGGAAAACTG GAAATGACTT TGGAAATCGT GGCTGAACAC
    GAGCATGAAG AGCGGCCAGC TGGCGTGGGT CGGGATGAGC CAAACATGAA CCCCAAGCTG
    GAGGACCCCA AGCGCCCAGA GACCTCCTTC CTGTGGTTCA CCTCCCCGTA CAAGACCCTG
    AAGTACATCC TGTGGCGCCG GTACAAGTGG CTGCTCATCC TGGCCATCGT GCTCTTTGTT
    TTGCTGCTCT TCCTTGGGAT CTTCATCTAC GCCTTCCCGA ACTACGCTGC TATGAAGCTG
    GTGAAACCTT TCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150239
    Clone ID Related Accession (Same CDS sequence) XM_025149867.1
    Accession Version XM_025149867.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGATGC GTTTCCTGCT CTCACGAAGC CTCTGAAGGC CACAGACCTG
    GACGACGGGC TGGGCTTCCT GCCGACCTTC GGCCCCTGCT ACGTCAACCT GTACGGCAGC
    CCCCGTGAGT TCACCGGCTT CCCGGATCCG TACGAGGCGC TCAATTTAGG AAAGGGCGAG
    GGCGTCGCGT ACCGCGGCCG GGTGCTGCTG GAGCTGGAGA CCAAGCTGGC GGAGCACGGG
    GAGCTGAAGG TGGAGGACAT CTGTGCGGAT GACATCCTGC GGGTGGAGAA GTACCTGCGG
    CGGAGGAAGT ACTCCCTCTT TGCTGCCTTC TACTCGGCCA CCATGCTGCA GGACGTGGAC
    GATGCCATCC AGTTTGAGGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CAACACCTGC
    TTGCCCTTGG CCTCCACCAC GCAGTACAGC CGGGCCGTGT TCGATGGTTG CCAGTATTAC
    TACCTGCCCT GGGGCAACGT GAAGCCCGTG GTGGTGCTGT CCTCCTATTG GGAAGATATC
    AGCTACCGAA CCGACACTCA GAACCTCCTC CAGCACGCAG CAGACAGGCT GGAAGCCAAC
    CTGGAGCGGG TCCACCTCGC GCTCAAGGCC AACGGCGCGC CGGGCGAGCT GGAGTCCCTG
    GCTGCCCAGC TGATGGACGA CGTGATTGCA GACTGCAGTG TTCCGCTGCC CGACGTGCTG
    GGGAAGGCGG CCAGCACACA CCTGGACCAG AACCTGTACC GCTTCCGCAG CACCAACCTG
    GAGCAGATGG TGCGGGCGGC CCTGAGGCTG AAGCACGAGG AGCTGAGCCT GCCGGCCGTG
    CTGGAGCAGG CGGAGGACTG GCTGTGCCGG CTGCGTGCCA TGGCTGAGGA GCCCCAGAAC
    AGCCTCCCAG ACGTGGTGAT CTGGATGCTG CGGGGTGAGA AGCGCGTTGC CTACGCCCGC
    GTGCCGGCCC GCGAGGTGCT CTACTCCCAA AGTGGCCCCA ACTGCTGCGG TAGGAACTGT
    GGCAAGCTGC AGACCATCTT CCTGAAGTAC CCCCAGGAGG AGGCGATGGG GCCGCGCATC
    CCAGCCCAGA TCCGCGTCCA GCTCTGGTTT GGGCTCTCTG TCAATGAGAA GGAGTTCAAC
    CAATACGCTG AGGGGAAGCT CTCCGTCTTT GCTGAAACCT ACGAGAACCA GACCAAGCTG
    GCGCTGGTGG GGAACTGGGG CACCACCGGC CTGACTTACC CCAAATACTC CGATGTCACC
    GGCAAGATCA AGCTCCCCAA GGACAGCTTC CGGCCCTCCA CGGGCTGGAC CTGGGCTGGT
    GATTGGTTCA TCTGCCCAGA GAAGACGATG CTGCACGAGG CAGACGCGGG GCACCTGAGC
    TTCGTGGAGG AGGTGTTTGA GAACCAGGTG CGGCTGCCGG GCGGGCAGTG GATCCACATG
    TCCGATGCCT ACACCGATGT GAATGGGGAG AAGGTTCTGC CCAAGGATGA GATCGAGTGT
    CCTCCGGGCT GGAAATGGGA AGACGTGGAG TGGGACACTG ACCTCAACAG AGCCGTGGAT
    GAGAAAGGCT GGGAGTACGG CATCACCATC CCTCCGGACC GCCGGCCCAA GGCCTGGGTG
    CCAGCTGAGA AGATGTACCA CACCAACCGG CGCCGGCGCT GGGTGCGACT GCGCAGCAGG
    GACCTGGCCC AGATGGAGAC TGTCAGGAAG CACAAGCAGG ACGAGCAGGA CGGCGAGGGC
    TGGGAATACG CCTCGCTGCT GGGCTGGCGC TTCCACCTGC GGCCGCGTCG CACCGATGCC
    TTCCGCCGCC GCCGTTGGAG ACGTAGGATG GAGCCCTTGG AGCGCACCGG GGCTGCTGCT
    GTGTTCGCCT TGGAGGGGGC CCTGGGAGGA ATGACGGACG ACAAAAGCGA CGATGGCAAA
    TCCGTCTCCA CGCTGAGTTT CGGCGTCAAC AGACCCACCA TCTCCTGCAT CTTCGACTAT
    GGGAACCGCT ACCACCTGCG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCCATGGAC
    AAGGACAGCT TCTCGGACCC CTATGCCATC GTTTCCTTCC TCCACCAAAG CCAGAAGACG
    GTGGTGATCA AGAACACCCT GAACCCCACG TGGGACCAGA CGCTCATCTT CTACGAGATA
    GAGATCTTCG GAGACCCCAA GAACGTGTCT GACTGCCCTC CCAGCATCGT GGTGGAGATA
    TACGACCACG ACACCTACGG CGCTGATGAG TTCATGGGGC GCTGCGTGTG CCGGCCCAGC
    CTGCAGCGCG CGCCGCGCCT CTCCTGGCAC CCCGTGCTCA AAGGCGGCAG GAACGTCGGC
    GAGCTGCTGG CTGCCTTCGA GCTCATCCAG AGGGAGAAGC CGGCTGTCCA CCACATCCCT
    GGCTTCGAGA ACGAGCTGTC CTCCGCTCTG GATGAGTCTG AGGACTCTGA CCTGCCCTAC
    CCACCGCCCC AACGGGAACC CAACATCTTC ATGGTCCCCC AAGGGATCAA ACCGGTCCTG
    CAGCGCACGG CCATCGAGAT CCTGGCCTGG GGGCTGAGGA ACCTCAAGAG CTACCAGCTG
    GCCAGCGTCA CCTCACCCAG CCTGCTGGTG GAGTGCGGGG GGCAGCTCGT GCAGTCCTGT
    GTCATCAAGA ACGTCAAGAA AAACCCCAAC TTTGATGTCT GTGTGCTCTT CATGGAAGTG
    CGTCTGCCCA GCGAGAGCCT CTACAGTCCC CCCATCATCA TCAAAATCAT CGACAACCGT
    CCGTTCGGCC GCAGGCCCGT GGTGGGGCAG TGCACCATCC GCTCACTGGA GGAATTCTAC
    TGTGACCCCT ATGGTGAGGA GACGGATGGT GCCCAGGAGC AGGCAGATGA TGTGAGCCTC
    ACGCCCAGGG ATGATGTCCT CATTGACATT GATGACAAAG AGCCTCTCCT TCCCACCCAG
    CTTGTGGAGG GCGTGACCAG CTCAGCGCTA ATTAACCCGC CAGCTTCTAG GTCCGAGCCT
    CAAGAGGAAG AATTCATTGA CTGGTGGAGC AAATTCTACG CTTCCACAGG AGAGAGGGAG
    AAGTGTGGCT CCTACTTGGA GAAGGGCTTT GACACCCTGC AGGTCTATGA GACGGAGCTG
    GAGCGCGTGG AGGCCTTCGA GCAGCTCTCT GACTTCTGCC ACACCTTCAG GCTGTACCGT
    GGCCGGGTGC AGGATGCCAA CGATGATCCC TCAGTTGTGG GCGAGTTCAA GGGCTCCTTC
    AAAATCTACC CTCTGCCCGA TGACCCACGC GTGCCGCTGC CGCCGCGGCA GTTCCACCAA
    CTGCCAGCCA GGGGTCCCCA GGAGTGTCTC GTCAGGGTCT ACATCATCCG TGCCTTCGGT
    CTGCAGCCCA AGGATGCCAA TGGGAAGTGC GATCCGTATG TGAAGATTTC AGTGGGAAAG
    AAATCCATAA ACGACCAAGA AAATTACCTC CCCTCTACGC TGGAGCCTGT CTTTGGAAAG
    ATGTTTGAGT TGAGCTGCAC TCTGCCCCTG GAGAAGGACC TGAAGGTCAC ACTCTATGAC
    TACGACCTCC TGTCCAAGGA TGAGAAGATT GGGGAGACCG TCATCGACCT GGAGAATCGG
    TTCCTGTCGA AATATGGGGC GCGCTGTGGG CTTCCCCAGA CCTACTGCAT CTCTGGGCCC
    AACCAGTGGC GAGACCAGCT CCGTCCCTCC CAACTGCTCC ACCTCTTCTC CCTGCAGCAC
    CACTACAAGG CTCCCACCTA CACGTCCGAC CGGCTCATCT TCCGGGAGCA GGAGTACATC
    CTCTCCGAGC TGGAGGATGG GAAACCCCCC AACCCTCACC TGGGGCCGGC AGAGGAGCGG
    CTGGCCCTGC ATGCACTGCG GAAACAAGGG CTGGTGCCGG AGCACGTGGA GACCCGGCCC
    CTCTACAGCC CCCTGCAGCC AGAGATCGAG CAGGGAAAGC TACAGATGTG GGTGGACCTG
    TTTCCGAAAT CCTTGGGTCA GCCTGGCCCC CCTTTCAACA TCACACCACG CAAAGCCAAG
    AGGTTCTACT TGCGCTGCAT CATCTGGAAC ACCAGGGACG TCATCCTCGA TGACCTCAGC
    ATCACTGGGG AGAAGATGAG TGACATCTAT GTCAAAGGCT GGCTGGTTGG CCACGAGGAG
    AACAAGCAGA AGACAGACGT GCACTACAGG TCGATGGGAG GAGAAGGCAA CTTCAACTGG
    AGGTTCATCT TCCCCTTCGA CTATCTCCCT GCCGAGCAGA TGTGTTGCGT TGCAAAGAAG
    GAGCATTTCT GGAGCTTGGA TAAGACGGAA AACAAGATTT CACCGCAGCT GATCTTCCAG
    ATCTGGGACA ATGACAAGTT CTCCTTCGAT GACTATTTGG GCTCCATCCA GATGGATCTC
    AACAGGATGC CCAAGCCTGC CAAGACTGCT GAGAAGTGCT CCTTAGAGCT CATAGATGAC
    AGCTTGTCTG CAAGCCGCTT TGTGTCCCTC TTTGAGCAGA AGACTGTCAA GGGCTGGTGG
    CCCTGCATTG CAGAGCAGGA TGAGAAGAAG ATCCTGGCAG GAAAACTGGA AATGACTTTG
    GAAATCGTGG CTGAACACGA GCATGAAGAG CGGCCAGCTG GCGTGGGTCG GGATGAGCCA
    AACATGAACC CCAAGCTGGA GGACCCCAAG CGCCCAGAGA CCTCCTTCCT GTGGTTCACC
    TCCCCGTACA AGACCCTGAA GTACATCCTG TGGCGCCGGT ACAAGTGGCT GCTCATCCTG
    GCCATCGTGC TCTTTGTTTT GCTGCTCTTC CTTGGGATCT TCATCTACGC CTTCCCGAAC
    TACGCTGCTA TGAAGCTGGT GAAACCTTTC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005635.1
    CDS647..7102
    Translation

    Target ORF information:

    RefSeq Version XM_025149867.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149867.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGATGC GTTTCCTGCT CTCACGAAGC CTCTGAAGGC CACAGACCTG
    GACGACGGGC TGGGCTTCCT GCCGACCTTC GGCCCCTGCT ACGTCAACCT GTACGGCAGC
    CCCCGTGAGT TCACCGGCTT CCCGGATCCG TACGAGGCGC TCAATTTAGG AAAGGGCGAG
    GGCGTCGCGT ACCGCGGCCG GGTGCTGCTG GAGCTGGAGA CCAAGCTGGC GGAGCACGGG
    GAGCTGAAGG TGGAGGACAT CTGTGCGGAT GACATCCTGC GGGTGGAGAA GTACCTGCGG
    CGGAGGAAGT ACTCCCTCTT TGCTGCCTTC TACTCGGCCA CCATGCTGCA GGACGTGGAC
    GATGCCATCC AGTTTGAGGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CAACACCTGC
    TTGCCCTTGG CCTCCACCAC GCAGTACAGC CGGGCCGTGT TCGATGGTTG CCAGTATTAC
    TACCTGCCCT GGGGCAACGT GAAGCCCGTG GTGGTGCTGT CCTCCTATTG GGAAGATATC
    AGCTACCGAA CCGACACTCA GAACCTCCTC CAGCACGCAG CAGACAGGCT GGAAGCCAAC
    CTGGAGCGGG TCCACCTCGC GCTCAAGGCC AACGGCGCGC CGGGCGAGCT GGAGTCCCTG
    GCTGCCCAGC TGATGGACGA CGTGATTGCA GACTGCAGTG TTCCGCTGCC CGACGTGCTG
    GGGAAGGCGG CCAGCACACA CCTGGACCAG AACCTGTACC GCTTCCGCAG CACCAACCTG
    GAGCAGATGG TGCGGGCGGC CCTGAGGCTG AAGCACGAGG AGCTGAGCCT GCCGGCCGTG
    CTGGAGCAGG CGGAGGACTG GCTGTGCCGG CTGCGTGCCA TGGCTGAGGA GCCCCAGAAC
    AGCCTCCCAG ACGTGGTGAT CTGGATGCTG CGGGGTGAGA AGCGCGTTGC CTACGCCCGC
    GTGCCGGCCC GCGAGGTGCT CTACTCCCAA AGTGGCCCCA ACTGCTGCGG TAGGAACTGT
    GGCAAGCTGC AGACCATCTT CCTGAAGTAC CCCCAGGAGG AGGCGATGGG GCCGCGCATC
    CCAGCCCAGA TCCGCGTCCA GCTCTGGTTT GGGCTCTCTG TCAATGAGAA GGAGTTCAAC
    CAATACGCTG AGGGGAAGCT CTCCGTCTTT GCTGAAACCT ACGAGAACCA GACCAAGCTG
    GCGCTGGTGG GGAACTGGGG CACCACCGGC CTGACTTACC CCAAATACTC CGATGTCACC
    GGCAAGATCA AGCTCCCCAA GGACAGCTTC CGGCCCTCCA CGGGCTGGAC CTGGGCTGGT
    GATTGGTTCA TCTGCCCAGA GAAGACGATG CTGCACGAGG CAGACGCGGG GCACCTGAGC
    TTCGTGGAGG AGGTGTTTGA GAACCAGGTG CGGCTGCCGG GCGGGCAGTG GATCCACATG
    TCCGATGCCT ACACCGATGT GAATGGGGAG AAGGTTCTGC CCAAGGATGA GATCGAGTGT
    CCTCCGGGCT GGAAATGGGA AGACGTGGAG TGGGACACTG ACCTCAACAG AGCCGTGGAT
    GAGAAAGGCT GGGAGTACGG CATCACCATC CCTCCGGACC GCCGGCCCAA GGCCTGGGTG
    CCAGCTGAGA AGATGTACCA CACCAACCGG CGCCGGCGCT GGGTGCGACT GCGCAGCAGG
    GACCTGGCCC AGATGGAGAC TGTCAGGAAG CACAAGCAGG ACGAGCAGGA CGGCGAGGGC
    TGGGAATACG CCTCGCTGCT GGGCTGGCGC TTCCACCTGC GGCCGCGTCG CACCGATGCC
    TTCCGCCGCC GCCGTTGGAG ACGTAGGATG GAGCCCTTGG AGCGCACCGG GGCTGCTGCT
    GTGTTCGCCT TGGAGGGGGC CCTGGGAGGA ATGACGGACG ACAAAAGCGA CGATGGCAAA
    TCCGTCTCCA CGCTGAGTTT CGGCGTCAAC AGACCCACCA TCTCCTGCAT CTTCGACTAT
    GGGAACCGCT ACCACCTGCG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCCATGGAC
    AAGGACAGCT TCTCGGACCC CTATGCCATC GTTTCCTTCC TCCACCAAAG CCAGAAGACG
    GTGGTGATCA AGAACACCCT GAACCCCACG TGGGACCAGA CGCTCATCTT CTACGAGATA
    GAGATCTTCG GAGACCCCAA GAACGTGTCT GACTGCCCTC CCAGCATCGT GGTGGAGATA
    TACGACCACG ACACCTACGG CGCTGATGAG TTCATGGGGC GCTGCGTGTG CCGGCCCAGC
    CTGCAGCGCG CGCCGCGCCT CTCCTGGCAC CCCGTGCTCA AAGGCGGCAG GAACGTCGGC
    GAGCTGCTGG CTGCCTTCGA GCTCATCCAG AGGGAGAAGC CGGCTGTCCA CCACATCCCT
    GGCTTCGAGA ACGAGCTGTC CTCCGCTCTG GATGAGTCTG AGGACTCTGA CCTGCCCTAC
    CCACCGCCCC AACGGGAACC CAACATCTTC ATGGTCCCCC AAGGGATCAA ACCGGTCCTG
    CAGCGCACGG CCATCGAGAT CCTGGCCTGG GGGCTGAGGA ACCTCAAGAG CTACCAGCTG
    GCCAGCGTCA CCTCACCCAG CCTGCTGGTG GAGTGCGGGG GGCAGCTCGT GCAGTCCTGT
    GTCATCAAGA ACGTCAAGAA AAACCCCAAC TTTGATGTCT GTGTGCTCTT CATGGAAGTG
    CGTCTGCCCA GCGAGAGCCT CTACAGTCCC CCCATCATCA TCAAAATCAT CGACAACCGT
    CCGTTCGGCC GCAGGCCCGT GGTGGGGCAG TGCACCATCC GCTCACTGGA GGAATTCTAC
    TGTGACCCCT ATGGTGAGGA GACGGATGGT GCCCAGGAGC AGGCAGATGA TGTGAGCCTC
    ACGCCCAGGG ATGATGTCCT CATTGACATT GATGACAAAG AGCCTCTCCT TCCCACCCAG
    CTTGTGGAGG GCGTGACCAG CTCAGCGCTA ATTAACCCGC CAGCTTCTAG GTCCGAGCCT
    CAAGAGGAAG AATTCATTGA CTGGTGGAGC AAATTCTACG CTTCCACAGG AGAGAGGGAG
    AAGTGTGGCT CCTACTTGGA GAAGGGCTTT GACACCCTGC AGGTCTATGA GACGGAGCTG
    GAGCGCGTGG AGGCCTTCGA GCAGCTCTCT GACTTCTGCC ACACCTTCAG GCTGTACCGT
    GGCCGGGTGC AGGATGCCAA CGATGATCCC TCAGTTGTGG GCGAGTTCAA GGGCTCCTTC
    AAAATCTACC CTCTGCCCGA TGACCCACGC GTGCCGCTGC CGCCGCGGCA GTTCCACCAA
    CTGCCAGCCA GGGGTCCCCA GGAGTGTCTC GTCAGGGTCT ACATCATCCG TGCCTTCGGT
    CTGCAGCCCA AGGATGCCAA TGGGAAGTGC GATCCGTATG TGAAGATTTC AGTGGGAAAG
    AAATCCATAA ACGACCAAGA AAATTACCTC CCCTCTACGC TGGAGCCTGT CTTTGGAAAG
    ATGTTTGAGT TGAGCTGCAC TCTGCCCCTG GAGAAGGACC TGAAGGTCAC ACTCTATGAC
    TACGACCTCC TGTCCAAGGA TGAGAAGATT GGGGAGACCG TCATCGACCT GGAGAATCGG
    TTCCTGTCGA AATATGGGGC GCGCTGTGGG CTTCCCCAGA CCTACTGCAT CTCTGGGCCC
    AACCAGTGGC GAGACCAGCT CCGTCCCTCC CAACTGCTCC ACCTCTTCTC CCTGCAGCAC
    CACTACAAGG CTCCCACCTA CACGTCCGAC CGGCTCATCT TCCGGGAGCA GGAGTACATC
    CTCTCCGAGC TGGAGGATGG GAAACCCCCC AACCCTCACC TGGGGCCGGC AGAGGAGCGG
    CTGGCCCTGC ATGCACTGCG GAAACAAGGG CTGGTGCCGG AGCACGTGGA GACCCGGCCC
    CTCTACAGCC CCCTGCAGCC AGAGATCGAG CAGGGAAAGC TACAGATGTG GGTGGACCTG
    TTTCCGAAAT CCTTGGGTCA GCCTGGCCCC CCTTTCAACA TCACACCACG CAAAGCCAAG
    AGGTTCTACT TGCGCTGCAT CATCTGGAAC ACCAGGGACG TCATCCTCGA TGACCTCAGC
    ATCACTGGGG AGAAGATGAG TGACATCTAT GTCAAAGGCT GGCTGGTTGG CCACGAGGAG
    AACAAGCAGA AGACAGACGT GCACTACAGG TCGATGGGAG GAGAAGGCAA CTTCAACTGG
    AGGTTCATCT TCCCCTTCGA CTATCTCCCT GCCGAGCAGA TGTGTTGCGT TGCAAAGAAG
    GAGCATTTCT GGAGCTTGGA TAAGACGGAA AACAAGATTT CACCGCAGCT GATCTTCCAG
    ATCTGGGACA ATGACAAGTT CTCCTTCGAT GACTATTTGG GCTCCATCCA GATGGATCTC
    AACAGGATGC CCAAGCCTGC CAAGACTGCT GAGAAGTGCT CCTTAGAGCT CATAGATGAC
    AGCTTGTCTG CAAGCCGCTT TGTGTCCCTC TTTGAGCAGA AGACTGTCAA GGGCTGGTGG
    CCCTGCATTG CAGAGCAGGA TGAGAAGAAG ATCCTGGCAG GAAAACTGGA AATGACTTTG
    GAAATCGTGG CTGAACACGA GCATGAAGAG CGGCCAGCTG GCGTGGGTCG GGATGAGCCA
    AACATGAACC CCAAGCTGGA GGACCCCAAG CGCCCAGAGA CCTCCTTCCT GTGGTTCACC
    TCCCCGTACA AGACCCTGAA GTACATCCTG TGGCGCCGGT ACAAGTGGCT GCTCATCCTG
    GCCATCGTGC TCTTTGTTTT GCTGCTCTTC CTTGGGATCT TCATCTACGC CTTCCCGAAC
    TACGCTGCTA TGAAGCTGGT GAAACCTTTC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150240
    Clone ID Related Accession (Same CDS sequence) XM_025149868.1
    Accession Version XM_025149868.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGTGGA CGACGGGCTG GGCTTCCTGC CGACCTTCGG CCCCTGCTAC
    GTCAACCTGT ACGGCAGCCC CCGTGAGTTC ACCGGCTTCC CGGATCCGTA CGAGGCGCTC
    AATTTAGGAA AGGGCGAGGG CGTCGCGTAC CGCGGCCGGG TGCTGCTGGA GCTGGAGACC
    AAGCTGGCGG AGCACGGGGA GCTGAAGGTG GAGGACATCT GTGCGGATGA CATCCTGCGG
    GTGGAGAAGT ACCTGCGGCG GAGGAAGTAC TCCCTCTTTG CTGCCTTCTA CTCGGCCACC
    ATGCTGCAGG ACGTGGACGA TGCCATCCAG TTTGAGGTCA GCATCGGCAA CTACGGCAAC
    AAGTTCGACA ACACCTGCTT GCCCTTGGCC TCCACCACGC AGTACAGCCG GGCCGTGTTC
    GATGGTTGCC AGTATTACTA CCTGCCCTGG GGCAACGTGA AGCCCGTGGT GGTGCTGTCC
    TCCTATTGGG AAGATATCAG CTACCGAACC GACACTCAGA ACCTCCTCCA GCACGCAGCA
    GACAGGCTGG AAGCCAACCT GGAGCGGGTC CACCTCGCGC TCAAGGCCAA CGGCGCGCCG
    GGCGAGCTGG AGTCCCTGGC TGCCCAGCTG ATGGACGACG TGATTGCAGA CTGCAGTGTT
    CCGCTGCCCG ACGTGCTGGG GAAGGCGGCC AGCACACACC TGGACCAGAA CCTGTACCGC
    TTCCGCAGCA CCAACCTGGA GCAGATGGTG CGGGCGGCCC TGAGGCTGAA GCACGAGGAG
    CTGAGCCTGC CGGCCGTGCT GGAGCAGGCG GAGGACTGGC TGTGCCGGCT GCGTGCCATG
    GCTGAGGAGC CCCAGAACAG CCTCCCAGAC GTGGTGATCT GGATGCTGCG GGGTGAGAAG
    CGCGTTGCCT ACGCCCGCGT GCCGGCCCGC GAGGTGCTCT ACTCCCAAAG TGGCCCCAAC
    TGCTGCGGTA GGAACTGTGG CAAGCTGCAG ACCATCTTCC TGAAGTACCC CCAGGAGGAG
    GCGATGGGGC CGCGCATCCC AGCCCAGATC CGCGTCCAGC TCTGGTTTGG GCTCTCTGTC
    AATGAGAAGG AGTTCAACCA ATACGCTGAG GGGAAGCTCT CCGTCTTTGC TGAAACCTAC
    GAGAACCAGA CCAAGCTGGC GCTGGTGGGG AACTGGGGCA CCACCGGCCT GACTTACCCC
    AAATACTCCG ATGTCACCGG CAAGATCAAG CTCCCCAAGG ACAGCTTCCG GCCCTCCACG
    GGCTGGACCT GGGCTGGTGA TTGGTTCATC TGCCCAGAGA AGACGATGCT GCACGAGGCA
    GACGCGGGGC ACCTGAGCTT CGTGGAGGAG GTGTTTGAGA ACCAGGTGCG GCTGCCGGGC
    GGGCAGTGGA TCCACATGTC CGATGCCTAC ACCGATGTGA ATGGGGAGAA GGTTCTGCCC
    AAGGATGAGA TCGAGTGTCC TCCGGGCTGG AAATGGGAAG ACGTGGAGTG GGACACTGAC
    CTCAACAGAG CCGTGGATGA GAAAGGCTGG GAGTACGGCA TCACCATCCC TCCGGACCGC
    CGGCCCAAGG CCTGGGTGCC AGCTGAGAAG ATGTACCACA CCAACCGGCG CCGGCGCTGG
    GTGCGACTGC GCAGCAGGGA CCTGGCCCAG ATGGAGACTG TCAGGAAGCA CAAGCAGGAC
    GAGCAGGACG GCGAGGGCTG GGAATACGCC TCGCTGCTGG GCTGGCGCTT CCACCTGCGG
    CCGCGTCGCA CCGATGCCTT CCGCCGCCGC CGTTGGAGAC GTAGGATGGA GCCCTTGGAG
    CGCACCGGGG CTGCTGCTGT GTTCGCCTTG GAGGGGGCCC TGGGAGGAAT GACGGACGAC
    AAAAGCGACG ATGGCAAATC CGTCTCCACG CTGAGTTTCG GCGTCAACAG ACCCACCATC
    TCCTGCATCT TCGACTATGG GAACCGCTAC CACCTGCGCT GCTACATGTA CCAGGCACGG
    GACCTGGCTG CCATGGACAA GGACAGCTTC TCGGACCCCT ATGCCATCGT TTCCTTCCTC
    CACCAAAGCC AGAAGACGGT GGTGATCAAG AACACCCTGA ACCCCACGTG GGACCAGACG
    CTCATCTTCT ACGAGATAGA GATCTTCGGA GACCCCAAGA ACGTGTCTGA CTGCCCTCCC
    AGCATCGTGG TGGAGATATA CGACCACGAC ACCTACGGCG CTGATGAGTT CATGGGGCGC
    TGCGTGTGCC GGCCCAGCCT GCAGCGCGCG CCGCGCCTCT CCTGGCACCC CGTGCTCAAA
    GGCGGCAGGA ACGTCGGCGA GCTGCTGGCT GCCTTCGAGC TCATCCAGAG GGAGAAGCCG
    GCTGTCCACC ACATCCCTGG CTTCGAGAAC GAGCTGTCCT CCGCTCTGGA TGAGCTCTGT
    GCCCCTGCCT TGTTCTACGA GGGGCTCCTC CGATGGTCTG AGGACTCTGA CCTGCCCTAC
    CCACCGCCCC AACGGGAACC CAACATCTTC ATGGTCCCCC AAGGGATCAA ACCGGTCCTG
    CAGCGCACGG CCATCGAGAT CCTGGCCTGG GGGCTGAGGA ACCTCAAGAG CTACCAGCTG
    GCCAGCGTCA CCTCACCCAG CCTGCTGGTG GAGTGCGGGG GGCAGCTCGT GCAGTCCTGT
    GTCATCAAGA ACGTCAAGAA AAACCCCAAC TTTGATGTCT GTGTGCTCTT CATGGAAGTG
    CGTCTGCCCA GCGAGAGCCT CTACAGTCCC CCCATCATCA TCAAAATCAT CGACAACCGT
    CCGTTCGGCC GCAGGCCCGT GGTGGGGCAG TGCACCATCC GCTCACTGGA GGAATTCTAC
    TGTGACCCCT ATGGTGAGGA GACGGATGGT GCCCAGGAGC AGGCAGATGA TGTGAGCCTC
    ACGCCCAGGG ATGATGTCCT CATTGACATT GATGACAAAG AGCCTCTCCT TCCCACCCAG
    CTTGTGGAGG GCGTGACCAG CTCAGCGCTA ATTAACCCGC CAGCTTCTAG GTCCGAGCCT
    CAAGAGGAAG AATTCATTGA CTGGTGGAGC AAATTCTACG CTTCCACAGG AGAGAGGGAG
    AAGTGTGGCT CCTACTTGGA GAAGGGCTTT GACACCCTGC AGGTCTATGA GACGGAGCTG
    GAGCGCGTGG AGGCCTTCGA GCAGCTCTCT GACTTCTGCC ACACCTTCAG GCTGTACCGT
    GGCCGGGTGC AGGATGCCAA CGATGATCCC TCAGTTGTGG GCGAGTTCAA GGGCTCCTTC
    AAAATCTACC CTCTGCCCGA TGACCCACGC GTGCCGCTGC CGCCGCGGCA GTTCCACCAA
    CTGCCAGCCA GGGGTCCCCA GGAGTGTCTC GTCAGGGTCT ACATCATCCG TGCCTTCGGT
    CTGCAGCCCA AGGATGCCAA TGGGAAGTGC GATCCGTATG TGAAGATTTC AGTGGGAAAG
    AAATCCATAA ACGACCAAGA AAATTACCTC CCCTCTACGC TGGAGCCTGT CTTTGGAAAG
    ATGTTTGAGT TGAGCTGCAC TCTGCCCCTG GAGAAGGACC TGAAGGTCAC ACTCTATGAC
    TACGACCTCC TGTCCAAGGA TGAGAAGATT GGGGAGACCG TCATCGACCT GGAGAATCGG
    TTCCTGTCGA AATATGGGGC GCGCTGTGGG CTTCCCCAGA CCTACTGCAT CTCTGGGCCC
    AACCAGTGGC GAGACCAGCT CCGTCCCTCC CAACTGCTCC ACCTCTTCTC CCTGCAGCAC
    CACTACAAGG CTCCCACCTA CACGTCCGAC CGGCTCATCT TCCGGGAGCA GGAGTACATC
    CTCTCCGAGC TGGAGGATGG GAAACCCCCC AACCCTCACC TGGGGCCGGC AGAGGAGCGG
    CTGGCCCTGC ATGCACTGCG GAAACAAGGG CTGGTGCCGG AGCACGTGGA GACCCGGCCC
    CTCTACAGCC CCCTGCAGCC AGAGATCGAG CAGGGAAAGC TACAGATGTG GGTGGACCTG
    TTTCCGAAAT CCTTGGGTCA GCCTGGCCCC CCTTTCAACA TCACACCACG CAAAGCCAAG
    AGGTTCTACT TGCGCTGCAT CATCTGGAAC ACCAGGGACG TCATCCTCGA TGACCTCAGC
    ATCACTGGGG AGAAGATGAG TGACATCTAT GTCAAAGGCT GGCTGGTTGG CCACGAGGAG
    AACAAGCAGA AGACAGACGT GCACTACAGG TCGATGGGAG GAGAAGGCAA CTTCAACTGG
    AGGTTCATCT TCCCCTTCGA CTATCTCCCT GCCGAGCAGA TGTGTTGCGT TGCAAAGAAG
    GAGCATTTCT GGAGCTTGGA TAAGACGGAA AACAAGATTT CACCGCAGCT GATCTTCCAG
    ATCTGGGACA ATGACAAGTT CTCCTTCGAT GACTATTTGG GCTCCATCCA GATGGATCTC
    AACAGGATGC CCAAGCCTGC CAAGACTGCT GAGAAGTGCT CCTTAGAGCT CATAGATGAC
    AGCTTGTCTG CAAGCCGCTT TGTGTCCCTC TTTGAGCAGA AGACTGTCAA GGGCTGGTGG
    CCCTGCATTG CAGAGCAGGA TGAGAAGAAG ATCCTGGCAG GAAAACTGGA AATGACTTTG
    GAAATCGTGG CTGAACACGA GCATGAAGAG CGGCCAGCTG GCGTGGGTCG GGATGAGCCA
    AACATGAACC CCAAGCTGGA GGACCCCAAG CGCCCAGAGA CCTCCTTCCT GTGGTTCACC
    TCCCCGTACA AGACCCTGAA GTACATCCTG TGGCGCCGGT ACAAGTGGCT GCTCATCCTG
    GCCATCGTGC TCTTTGTTTT GCTGCTCTTC CTTGGGATCT TCATCTACGC CTTCCCGAAC
    TACGCTGCTA TGAAGCTGGT GAAACCTTTC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005636.1
    CDS648..7103
    Translation

    Target ORF information:

    RefSeq Version XM_025149868.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149868.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGTGGA CGACGGGCTG GGCTTCCTGC CGACCTTCGG CCCCTGCTAC
    GTCAACCTGT ACGGCAGCCC CCGTGAGTTC ACCGGCTTCC CGGATCCGTA CGAGGCGCTC
    AATTTAGGAA AGGGCGAGGG CGTCGCGTAC CGCGGCCGGG TGCTGCTGGA GCTGGAGACC
    AAGCTGGCGG AGCACGGGGA GCTGAAGGTG GAGGACATCT GTGCGGATGA CATCCTGCGG
    GTGGAGAAGT ACCTGCGGCG GAGGAAGTAC TCCCTCTTTG CTGCCTTCTA CTCGGCCACC
    ATGCTGCAGG ACGTGGACGA TGCCATCCAG TTTGAGGTCA GCATCGGCAA CTACGGCAAC
    AAGTTCGACA ACACCTGCTT GCCCTTGGCC TCCACCACGC AGTACAGCCG GGCCGTGTTC
    GATGGTTGCC AGTATTACTA CCTGCCCTGG GGCAACGTGA AGCCCGTGGT GGTGCTGTCC
    TCCTATTGGG AAGATATCAG CTACCGAACC GACACTCAGA ACCTCCTCCA GCACGCAGCA
    GACAGGCTGG AAGCCAACCT GGAGCGGGTC CACCTCGCGC TCAAGGCCAA CGGCGCGCCG
    GGCGAGCTGG AGTCCCTGGC TGCCCAGCTG ATGGACGACG TGATTGCAGA CTGCAGTGTT
    CCGCTGCCCG ACGTGCTGGG GAAGGCGGCC AGCACACACC TGGACCAGAA CCTGTACCGC
    TTCCGCAGCA CCAACCTGGA GCAGATGGTG CGGGCGGCCC TGAGGCTGAA GCACGAGGAG
    CTGAGCCTGC CGGCCGTGCT GGAGCAGGCG GAGGACTGGC TGTGCCGGCT GCGTGCCATG
    GCTGAGGAGC CCCAGAACAG CCTCCCAGAC GTGGTGATCT GGATGCTGCG GGGTGAGAAG
    CGCGTTGCCT ACGCCCGCGT GCCGGCCCGC GAGGTGCTCT ACTCCCAAAG TGGCCCCAAC
    TGCTGCGGTA GGAACTGTGG CAAGCTGCAG ACCATCTTCC TGAAGTACCC CCAGGAGGAG
    GCGATGGGGC CGCGCATCCC AGCCCAGATC CGCGTCCAGC TCTGGTTTGG GCTCTCTGTC
    AATGAGAAGG AGTTCAACCA ATACGCTGAG GGGAAGCTCT CCGTCTTTGC TGAAACCTAC
    GAGAACCAGA CCAAGCTGGC GCTGGTGGGG AACTGGGGCA CCACCGGCCT GACTTACCCC
    AAATACTCCG ATGTCACCGG CAAGATCAAG CTCCCCAAGG ACAGCTTCCG GCCCTCCACG
    GGCTGGACCT GGGCTGGTGA TTGGTTCATC TGCCCAGAGA AGACGATGCT GCACGAGGCA
    GACGCGGGGC ACCTGAGCTT CGTGGAGGAG GTGTTTGAGA ACCAGGTGCG GCTGCCGGGC
    GGGCAGTGGA TCCACATGTC CGATGCCTAC ACCGATGTGA ATGGGGAGAA GGTTCTGCCC
    AAGGATGAGA TCGAGTGTCC TCCGGGCTGG AAATGGGAAG ACGTGGAGTG GGACACTGAC
    CTCAACAGAG CCGTGGATGA GAAAGGCTGG GAGTACGGCA TCACCATCCC TCCGGACCGC
    CGGCCCAAGG CCTGGGTGCC AGCTGAGAAG ATGTACCACA CCAACCGGCG CCGGCGCTGG
    GTGCGACTGC GCAGCAGGGA CCTGGCCCAG ATGGAGACTG TCAGGAAGCA CAAGCAGGAC
    GAGCAGGACG GCGAGGGCTG GGAATACGCC TCGCTGCTGG GCTGGCGCTT CCACCTGCGG
    CCGCGTCGCA CCGATGCCTT CCGCCGCCGC CGTTGGAGAC GTAGGATGGA GCCCTTGGAG
    CGCACCGGGG CTGCTGCTGT GTTCGCCTTG GAGGGGGCCC TGGGAGGAAT GACGGACGAC
    AAAAGCGACG ATGGCAAATC CGTCTCCACG CTGAGTTTCG GCGTCAACAG ACCCACCATC
    TCCTGCATCT TCGACTATGG GAACCGCTAC CACCTGCGCT GCTACATGTA CCAGGCACGG
    GACCTGGCTG CCATGGACAA GGACAGCTTC TCGGACCCCT ATGCCATCGT TTCCTTCCTC
    CACCAAAGCC AGAAGACGGT GGTGATCAAG AACACCCTGA ACCCCACGTG GGACCAGACG
    CTCATCTTCT ACGAGATAGA GATCTTCGGA GACCCCAAGA ACGTGTCTGA CTGCCCTCCC
    AGCATCGTGG TGGAGATATA CGACCACGAC ACCTACGGCG CTGATGAGTT CATGGGGCGC
    TGCGTGTGCC GGCCCAGCCT GCAGCGCGCG CCGCGCCTCT CCTGGCACCC CGTGCTCAAA
    GGCGGCAGGA ACGTCGGCGA GCTGCTGGCT GCCTTCGAGC TCATCCAGAG GGAGAAGCCG
    GCTGTCCACC ACATCCCTGG CTTCGAGAAC GAGCTGTCCT CCGCTCTGGA TGAGCTCTGT
    GCCCCTGCCT TGTTCTACGA GGGGCTCCTC CGATGGTCTG AGGACTCTGA CCTGCCCTAC
    CCACCGCCCC AACGGGAACC CAACATCTTC ATGGTCCCCC AAGGGATCAA ACCGGTCCTG
    CAGCGCACGG CCATCGAGAT CCTGGCCTGG GGGCTGAGGA ACCTCAAGAG CTACCAGCTG
    GCCAGCGTCA CCTCACCCAG CCTGCTGGTG GAGTGCGGGG GGCAGCTCGT GCAGTCCTGT
    GTCATCAAGA ACGTCAAGAA AAACCCCAAC TTTGATGTCT GTGTGCTCTT CATGGAAGTG
    CGTCTGCCCA GCGAGAGCCT CTACAGTCCC CCCATCATCA TCAAAATCAT CGACAACCGT
    CCGTTCGGCC GCAGGCCCGT GGTGGGGCAG TGCACCATCC GCTCACTGGA GGAATTCTAC
    TGTGACCCCT ATGGTGAGGA GACGGATGGT GCCCAGGAGC AGGCAGATGA TGTGAGCCTC
    ACGCCCAGGG ATGATGTCCT CATTGACATT GATGACAAAG AGCCTCTCCT TCCCACCCAG
    CTTGTGGAGG GCGTGACCAG CTCAGCGCTA ATTAACCCGC CAGCTTCTAG GTCCGAGCCT
    CAAGAGGAAG AATTCATTGA CTGGTGGAGC AAATTCTACG CTTCCACAGG AGAGAGGGAG
    AAGTGTGGCT CCTACTTGGA GAAGGGCTTT GACACCCTGC AGGTCTATGA GACGGAGCTG
    GAGCGCGTGG AGGCCTTCGA GCAGCTCTCT GACTTCTGCC ACACCTTCAG GCTGTACCGT
    GGCCGGGTGC AGGATGCCAA CGATGATCCC TCAGTTGTGG GCGAGTTCAA GGGCTCCTTC
    AAAATCTACC CTCTGCCCGA TGACCCACGC GTGCCGCTGC CGCCGCGGCA GTTCCACCAA
    CTGCCAGCCA GGGGTCCCCA GGAGTGTCTC GTCAGGGTCT ACATCATCCG TGCCTTCGGT
    CTGCAGCCCA AGGATGCCAA TGGGAAGTGC GATCCGTATG TGAAGATTTC AGTGGGAAAG
    AAATCCATAA ACGACCAAGA AAATTACCTC CCCTCTACGC TGGAGCCTGT CTTTGGAAAG
    ATGTTTGAGT TGAGCTGCAC TCTGCCCCTG GAGAAGGACC TGAAGGTCAC ACTCTATGAC
    TACGACCTCC TGTCCAAGGA TGAGAAGATT GGGGAGACCG TCATCGACCT GGAGAATCGG
    TTCCTGTCGA AATATGGGGC GCGCTGTGGG CTTCCCCAGA CCTACTGCAT CTCTGGGCCC
    AACCAGTGGC GAGACCAGCT CCGTCCCTCC CAACTGCTCC ACCTCTTCTC CCTGCAGCAC
    CACTACAAGG CTCCCACCTA CACGTCCGAC CGGCTCATCT TCCGGGAGCA GGAGTACATC
    CTCTCCGAGC TGGAGGATGG GAAACCCCCC AACCCTCACC TGGGGCCGGC AGAGGAGCGG
    CTGGCCCTGC ATGCACTGCG GAAACAAGGG CTGGTGCCGG AGCACGTGGA GACCCGGCCC
    CTCTACAGCC CCCTGCAGCC AGAGATCGAG CAGGGAAAGC TACAGATGTG GGTGGACCTG
    TTTCCGAAAT CCTTGGGTCA GCCTGGCCCC CCTTTCAACA TCACACCACG CAAAGCCAAG
    AGGTTCTACT TGCGCTGCAT CATCTGGAAC ACCAGGGACG TCATCCTCGA TGACCTCAGC
    ATCACTGGGG AGAAGATGAG TGACATCTAT GTCAAAGGCT GGCTGGTTGG CCACGAGGAG
    AACAAGCAGA AGACAGACGT GCACTACAGG TCGATGGGAG GAGAAGGCAA CTTCAACTGG
    AGGTTCATCT TCCCCTTCGA CTATCTCCCT GCCGAGCAGA TGTGTTGCGT TGCAAAGAAG
    GAGCATTTCT GGAGCTTGGA TAAGACGGAA AACAAGATTT CACCGCAGCT GATCTTCCAG
    ATCTGGGACA ATGACAAGTT CTCCTTCGAT GACTATTTGG GCTCCATCCA GATGGATCTC
    AACAGGATGC CCAAGCCTGC CAAGACTGCT GAGAAGTGCT CCTTAGAGCT CATAGATGAC
    AGCTTGTCTG CAAGCCGCTT TGTGTCCCTC TTTGAGCAGA AGACTGTCAA GGGCTGGTGG
    CCCTGCATTG CAGAGCAGGA TGAGAAGAAG ATCCTGGCAG GAAAACTGGA AATGACTTTG
    GAAATCGTGG CTGAACACGA GCATGAAGAG CGGCCAGCTG GCGTGGGTCG GGATGAGCCA
    AACATGAACC CCAAGCTGGA GGACCCCAAG CGCCCAGAGA CCTCCTTCCT GTGGTTCACC
    TCCCCGTACA AGACCCTGAA GTACATCCTG TGGCGCCGGT ACAAGTGGCT GCTCATCCTG
    GCCATCGTGC TCTTTGTTTT GCTGCTCTTC CTTGGGATCT TCATCTACGC CTTCCCGAAC
    TACGCTGCTA TGAAGCTGGT GAAACCTTTC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150241
    Clone ID Related Accession (Same CDS sequence) XM_025149869.1
    Accession Version XM_025149869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGATGC GTTTCCTGCT CTCACGAAGC CTCTGAAGGC CACAGACCTG
    GACGACGGGC TGGGCTTCCT GCCGACCTTC GGCCCCTGCT ACGTCAACCT GTACGGCAGC
    CCCCGTGAGT TCACCGGCTT CCCGGATCCG TACGAGGCGC TCAATTTAGG AAAGGGCGAG
    GGCGTCGCGT ACCGCGGCCG GGTGCTGCTG GAGCTGGAGA CCAAGCTGGC GGAGCACGGG
    GAGCTGAAGG TGGAGGACAT CTGTGCGGAT GACATCCTGC GGGTGGAGAA GTACCTGCGG
    CGGAGGAAGT ACTCCCTCTT TGCTGCCTTC TACTCGGCCA CCATGCTGCA GGACGTGGAC
    GATGCCATCC AGTTTGAGGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CAACACCTGC
    TTGCCCTTGG CCTCCACCAC GCAGTACAGC CGGGCCGTGT TCGATGGTTG CCAGTATTAC
    TACCTGCCCT GGGGCAACGT GAAGCCCGTG GTGGTGCTGT CCTCCTATTG GGAAGATATC
    AGCTACCGAA CCGACACTCA GAACCTCCTC CAGCACGCAG CAGACAGGCT GGAAGCCAAC
    CTGGAGCGGG TCCACCTCGC GCTCAAGGCC AACGGCGCGC CGGGCGAGCT GGAGTCCCTG
    GCTGCCCAGC TGATGGACGA CGTGATTGCA GACTGCAGTG TTCCGCTGCC CGACGTGCTG
    GGGAAGGCGG CCAGCACACA CCTGGACCAG AACCTGTACC GCTTCCGCAG CACCAACCTG
    GAGCAGATGG TGCGGGCGGC CCTGAGGCTG AAGCACGAGG AGCTGAGCCT GCCGGCCGTG
    CTGGAGCAGG CGGAGGACTG GCTGTGCCGG CTGCGTGCCA TGGCTGAGGA GCCCCAGAAC
    AGCCTCCCAG ACGTGGTGAT CTGGATGCTG CGGGGTGAGA AGCGCGTTGC CTACGCCCGC
    GTGCCGGCCC GCGAGGTGCT CTACTCCCAA AGTGGCCCCA ACTGCTGCGG TAGGAACTGT
    GGCAAGCTGC AGACCATCTT CCTGAAGTAC CCCCAGGAGG AGGCGATGGG GCCGCGCATC
    CCAGCCCAGA TCCGCGTCCA GCTCTGGTTT GGGCTCTCTG TCAATGAGAA GGAGTTCAAC
    CAATACGCTG AGGGGAAGCT CTCCGTCTTT GCTGAAACCT ACGAGAACCA GACCAAGCTG
    GCGCTGGTGG GGAACTGGGG CACCACCGGC CTGACTTACC CCAAATACTC CGATGTCACC
    GGCAAGATCA AGCTCCCCAA GGACAGCTTC CGGCCCTCCA CGGGCTGGAC CTGGGCTGGT
    GATTGGTTCA TCTGCCCAGA GAAGACGATG CTGCACGAGG CAGACGCGGG GCACCTGAGC
    TTCGTGGAGG AGGTGTTTGA GAACCAGGTG CGGCTGCCGG GCGGGCAGTG GATCCACATG
    TCCGATGCCT ACACCGATGT GAATGGGGAG AAGGTTCTGC CCAAGGATGA GATCGAGTGT
    CCTCCGGGCT GGAAATGGGA AGACGTGGAG TGGGACACTG ACCTCAACAG AGCCGTGGAT
    GAGAAAGGCT GGGAGTACGG CATCACCATC CCTCCGGACC GCCGGCCCAA GGCCTGGGTG
    CCAGCTGAGA AGATGTACCA CACCAACCGG CGCCGGCGCT GGGTGCGACT GCGCAGCAGG
    GACCTGGCCC AGATGGAGAC TGTCAGGAAG CACAAGCAGG ACGAGCAGGA CGGCGAGGGC
    TGGGAATACG CCTCGCTGCT GGGCTGGCGC TTCCACCTGC GGCCGCGTCG CACCGATGCC
    TTCCGCCGCC GCCGTTGGAG ACGTAGGATG GAGCCCTTGG AGCGCACCGG GGCTGCTGCT
    GTGTTCGCCT TGGAGGGGGC CCTGGGAGGA ATGACGGACG ACAAAAGCGA CGATGGCAAA
    TCCGTCTCCA CGCTGAGTTT CGGCGTCAAC AGACCCACCA TCTCCTGCAT CTTCGACTAT
    GGGAACCGCT ACCACCTGCG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCCATGGAC
    AAGGACAGCT TCTCGGACCC CTATGCCATC GTTTCCTTCC TCCACCAAAG CCAGAAGACG
    GTGGTGATCA AGAACACCCT GAACCCCACG TGGGACCAGA CGCTCATCTT CTACGAGATA
    GAGATCTTCG GAGACCCCAA GAACGTGTCT GACTGCCCTC CCAGCATCGT GGTGGAGATA
    TACGACCACG ACACCTACGG CGCTGATGAG TTCATGGGGC GCTGCGTGTG CCGGCCCAGC
    CTGCAGCGCG CGCCGCGCCT CTCCTGGCAC CCCGTGCTCA AAGGCGGCAG GAACGTCGGC
    GAGCTGCTGG CTGCCTTCGA GCTCATCCAG AGGGAGAAGC CGGCTGTCCA CCACATCCCT
    GGCTTCGAGA ACGAGCTGTC CTCCGCTCTG GATGAGCTCT GTGCCCCTGC CTTGTTCTAC
    GAGGGGCTCC TCCGATGGTC TGAGGACTCT GACCTGCCCT ACCCACCGCC CCAACGGGAA
    CCCAACATCT TCATGGTCCC CCAAGGGATC AAACCGGTCC TGCAGCGCAC GGCCATCGAG
    ATCCTGGCCT GGGGGCTGAG GAACCTCAAG AGCTACCAGC TGGCCAGCGT CACCTCACCC
    AGCCTGCTGG TGGAGTGCGG GGGGCAGCTC GTGCAGTCCT GTGTCATCAA GAACGTCAAG
    AAAAACCCCA ACTTTGATGT CTGTGTGCTC TTCATGGAAG TGCGTCTGCC CAGCGAGAGC
    CTCTACAGTC CCCCCATCAT CATCAAAATC ATCGACAACC GTCCGTTCGG CCGCAGGCCC
    GTGGTGGGGC AGTGCACCAT CCGCTCACTG GAGGAATTCT ACTGTGACCC CTATGGTGAG
    GAGACGGATG GTGCCCAGGA GCAGGCAGAT GATGTGAGCC TCACGCCCAG GGATGATGTC
    CTCATTGACA TTGATGACAA AGAGCCTCTC CTTCCCACCC AGGAGGAAGA ATTCATTGAC
    TGGTGGAGCA AATTCTACGC TTCCACAGGA GAGAGGGAGA AGTGTGGCTC CTACTTGGAG
    AAGGGCTTTG ACACCCTGCA GGTCTATGAG ACGGAGCTGG AGCGCGTGGA GGCCTTCGAG
    CAGCTCTCTG ACTTCTGCCA CACCTTCAGG CTGTACCGTG GCCGGGTGCA GGATGCCAAC
    GATGATCCCT CAGTTGTGGG CGAGTTCAAG GGCTCCTTCA AAATCTACCC TCTGCCCGAT
    GACCCACGCG TGCCGCTGCC GCCGCGGCAG TTCCACCAAC TGCCAGCCAG GGGTCCCCAG
    GAGTGTCTCG TCAGGGTCTA CATCATCCGT GCCTTCGGTC TGCAGCCCAA GGATGCCAAT
    GGGAAGTGCG ATCCGTATGT GAAGATTTCA GTGGGAAAGA AATCCATAAA CGACCAAGAA
    AATTACCTCC CCTCTACGCT GGAGCCTGTC TTTGGAAAGA TGTTTGAGTT GAGCTGCACT
    CTGCCCCTGG AGAAGGACCT GAAGGTCACA CTCTATGACT ACGACCTCCT GTCCAAGGAT
    GAGAAGATTG GGGAGACCGT CATCGACCTG GAGAATCGGT TCCTGTCGAA ATATGGGGCG
    CGCTGTGGGC TTCCCCAGAC CTACTGCATC TCTGGGCCCA ACCAGTGGCG AGACCAGCTC
    CGTCCCTCCC AACTGCTCCA CCTCTTCTCC CTGCAGCACC ACTACAAGGC TCCCACCTAC
    ACGTCCGACC GGCTCATCTT CCGGGAGCAG GAGTACATCC TCTCCGAGCT GGAGGATGGG
    AAACCCCCCA ACCCTCACCT GGGGCCGGCA GAGGAGCGGC TGGCCCTGCA TGCACTGCGG
    AAACAAGGGC TGGTGCCGGA GCACGTGGAG ACCCGGCCCC TCTACAGCCC CCTGCAGCCA
    GAGATCGAGC AGGGAAAGCT ACAGATGTGG GTGGACCTGT TTCCGAAATC CTTGGGTCAG
    CCTGGCCCCC CTTTCAACAT CACACCACGC AAAGCCAAGA GGTTCTACTT GCGCTGCATC
    ATCTGGAACA CCAGGGACGT CATCCTCGAT GACCTCAGCA TCACTGGGGA GAAGATGAGT
    GACATCTATG TCAAAGGCTG GCTGGTTGGC CACGAGGAGA ACAAGCAGAA GACAGACGTG
    CACTACAGGT CGATGGGAGG AGAAGGCAAC TTCAACTGGA GGTTCATCTT CCCCTTCGAC
    TATCTCCCTG CCGAGCAGAT GTGTTGCGTT GCAAAGAAGG AGCATTTCTG GAGCTTGGAT
    AAGACGGAAA ACAAGATTTC ACCGCAGCTG ATCTTCCAGA TCTGGGACAA TGACAAGTTC
    TCCTTCGATG ACTATTTGGG CTCCATCCAG ATGGATCTCA ACAGGATGCC CAAGCCTGCC
    AAGACTGCTG AGAAGTGCTC CTTAGAGCTC ATAGATGACA GCTTGTCTGC AAGCCGCTTT
    GTGTCCCTCT TTGAGCAGAA GACTGTCAAG GGCTGGTGGC CCTGCATTGC AGAGCAGGAT
    GAGAAGAAGA TCCTGGCAGG AAAACTGGAA ATGACTTTGG AAATCGTGGC TGAACACGAG
    CATGAAGAGC GGCCAGCTGG CGTGGGTCGG GATGAGCCAA ACATGAACCC CAAGCTGGAG
    GACCCCAAGC GCCCAGAGAC CTCCTTCCTG TGGTTCACCT CCCCGTACAA GACCCTGAAG
    TACATCCTGT GGCGCCGGTA CAAGTGGCTG CTCATCCTGG CCATCGTGCT CTTTGTTTTG
    CTGCTCTTCC TTGGGATCTT CATCTACGCC TTCCCGAACT ACGCTGCTAT GAAGCTGGTG
    AAACCTTTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005637.1
    CDS648..7082
    Translation

    Target ORF information:

    RefSeq Version XM_025149869.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGATGC GTTTCCTGCT CTCACGAAGC CTCTGAAGGC CACAGACCTG
    GACGACGGGC TGGGCTTCCT GCCGACCTTC GGCCCCTGCT ACGTCAACCT GTACGGCAGC
    CCCCGTGAGT TCACCGGCTT CCCGGATCCG TACGAGGCGC TCAATTTAGG AAAGGGCGAG
    GGCGTCGCGT ACCGCGGCCG GGTGCTGCTG GAGCTGGAGA CCAAGCTGGC GGAGCACGGG
    GAGCTGAAGG TGGAGGACAT CTGTGCGGAT GACATCCTGC GGGTGGAGAA GTACCTGCGG
    CGGAGGAAGT ACTCCCTCTT TGCTGCCTTC TACTCGGCCA CCATGCTGCA GGACGTGGAC
    GATGCCATCC AGTTTGAGGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CAACACCTGC
    TTGCCCTTGG CCTCCACCAC GCAGTACAGC CGGGCCGTGT TCGATGGTTG CCAGTATTAC
    TACCTGCCCT GGGGCAACGT GAAGCCCGTG GTGGTGCTGT CCTCCTATTG GGAAGATATC
    AGCTACCGAA CCGACACTCA GAACCTCCTC CAGCACGCAG CAGACAGGCT GGAAGCCAAC
    CTGGAGCGGG TCCACCTCGC GCTCAAGGCC AACGGCGCGC CGGGCGAGCT GGAGTCCCTG
    GCTGCCCAGC TGATGGACGA CGTGATTGCA GACTGCAGTG TTCCGCTGCC CGACGTGCTG
    GGGAAGGCGG CCAGCACACA CCTGGACCAG AACCTGTACC GCTTCCGCAG CACCAACCTG
    GAGCAGATGG TGCGGGCGGC CCTGAGGCTG AAGCACGAGG AGCTGAGCCT GCCGGCCGTG
    CTGGAGCAGG CGGAGGACTG GCTGTGCCGG CTGCGTGCCA TGGCTGAGGA GCCCCAGAAC
    AGCCTCCCAG ACGTGGTGAT CTGGATGCTG CGGGGTGAGA AGCGCGTTGC CTACGCCCGC
    GTGCCGGCCC GCGAGGTGCT CTACTCCCAA AGTGGCCCCA ACTGCTGCGG TAGGAACTGT
    GGCAAGCTGC AGACCATCTT CCTGAAGTAC CCCCAGGAGG AGGCGATGGG GCCGCGCATC
    CCAGCCCAGA TCCGCGTCCA GCTCTGGTTT GGGCTCTCTG TCAATGAGAA GGAGTTCAAC
    CAATACGCTG AGGGGAAGCT CTCCGTCTTT GCTGAAACCT ACGAGAACCA GACCAAGCTG
    GCGCTGGTGG GGAACTGGGG CACCACCGGC CTGACTTACC CCAAATACTC CGATGTCACC
    GGCAAGATCA AGCTCCCCAA GGACAGCTTC CGGCCCTCCA CGGGCTGGAC CTGGGCTGGT
    GATTGGTTCA TCTGCCCAGA GAAGACGATG CTGCACGAGG CAGACGCGGG GCACCTGAGC
    TTCGTGGAGG AGGTGTTTGA GAACCAGGTG CGGCTGCCGG GCGGGCAGTG GATCCACATG
    TCCGATGCCT ACACCGATGT GAATGGGGAG AAGGTTCTGC CCAAGGATGA GATCGAGTGT
    CCTCCGGGCT GGAAATGGGA AGACGTGGAG TGGGACACTG ACCTCAACAG AGCCGTGGAT
    GAGAAAGGCT GGGAGTACGG CATCACCATC CCTCCGGACC GCCGGCCCAA GGCCTGGGTG
    CCAGCTGAGA AGATGTACCA CACCAACCGG CGCCGGCGCT GGGTGCGACT GCGCAGCAGG
    GACCTGGCCC AGATGGAGAC TGTCAGGAAG CACAAGCAGG ACGAGCAGGA CGGCGAGGGC
    TGGGAATACG CCTCGCTGCT GGGCTGGCGC TTCCACCTGC GGCCGCGTCG CACCGATGCC
    TTCCGCCGCC GCCGTTGGAG ACGTAGGATG GAGCCCTTGG AGCGCACCGG GGCTGCTGCT
    GTGTTCGCCT TGGAGGGGGC CCTGGGAGGA ATGACGGACG ACAAAAGCGA CGATGGCAAA
    TCCGTCTCCA CGCTGAGTTT CGGCGTCAAC AGACCCACCA TCTCCTGCAT CTTCGACTAT
    GGGAACCGCT ACCACCTGCG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCCATGGAC
    AAGGACAGCT TCTCGGACCC CTATGCCATC GTTTCCTTCC TCCACCAAAG CCAGAAGACG
    GTGGTGATCA AGAACACCCT GAACCCCACG TGGGACCAGA CGCTCATCTT CTACGAGATA
    GAGATCTTCG GAGACCCCAA GAACGTGTCT GACTGCCCTC CCAGCATCGT GGTGGAGATA
    TACGACCACG ACACCTACGG CGCTGATGAG TTCATGGGGC GCTGCGTGTG CCGGCCCAGC
    CTGCAGCGCG CGCCGCGCCT CTCCTGGCAC CCCGTGCTCA AAGGCGGCAG GAACGTCGGC
    GAGCTGCTGG CTGCCTTCGA GCTCATCCAG AGGGAGAAGC CGGCTGTCCA CCACATCCCT
    GGCTTCGAGA ACGAGCTGTC CTCCGCTCTG GATGAGCTCT GTGCCCCTGC CTTGTTCTAC
    GAGGGGCTCC TCCGATGGTC TGAGGACTCT GACCTGCCCT ACCCACCGCC CCAACGGGAA
    CCCAACATCT TCATGGTCCC CCAAGGGATC AAACCGGTCC TGCAGCGCAC GGCCATCGAG
    ATCCTGGCCT GGGGGCTGAG GAACCTCAAG AGCTACCAGC TGGCCAGCGT CACCTCACCC
    AGCCTGCTGG TGGAGTGCGG GGGGCAGCTC GTGCAGTCCT GTGTCATCAA GAACGTCAAG
    AAAAACCCCA ACTTTGATGT CTGTGTGCTC TTCATGGAAG TGCGTCTGCC CAGCGAGAGC
    CTCTACAGTC CCCCCATCAT CATCAAAATC ATCGACAACC GTCCGTTCGG CCGCAGGCCC
    GTGGTGGGGC AGTGCACCAT CCGCTCACTG GAGGAATTCT ACTGTGACCC CTATGGTGAG
    GAGACGGATG GTGCCCAGGA GCAGGCAGAT GATGTGAGCC TCACGCCCAG GGATGATGTC
    CTCATTGACA TTGATGACAA AGAGCCTCTC CTTCCCACCC AGGAGGAAGA ATTCATTGAC
    TGGTGGAGCA AATTCTACGC TTCCACAGGA GAGAGGGAGA AGTGTGGCTC CTACTTGGAG
    AAGGGCTTTG ACACCCTGCA GGTCTATGAG ACGGAGCTGG AGCGCGTGGA GGCCTTCGAG
    CAGCTCTCTG ACTTCTGCCA CACCTTCAGG CTGTACCGTG GCCGGGTGCA GGATGCCAAC
    GATGATCCCT CAGTTGTGGG CGAGTTCAAG GGCTCCTTCA AAATCTACCC TCTGCCCGAT
    GACCCACGCG TGCCGCTGCC GCCGCGGCAG TTCCACCAAC TGCCAGCCAG GGGTCCCCAG
    GAGTGTCTCG TCAGGGTCTA CATCATCCGT GCCTTCGGTC TGCAGCCCAA GGATGCCAAT
    GGGAAGTGCG ATCCGTATGT GAAGATTTCA GTGGGAAAGA AATCCATAAA CGACCAAGAA
    AATTACCTCC CCTCTACGCT GGAGCCTGTC TTTGGAAAGA TGTTTGAGTT GAGCTGCACT
    CTGCCCCTGG AGAAGGACCT GAAGGTCACA CTCTATGACT ACGACCTCCT GTCCAAGGAT
    GAGAAGATTG GGGAGACCGT CATCGACCTG GAGAATCGGT TCCTGTCGAA ATATGGGGCG
    CGCTGTGGGC TTCCCCAGAC CTACTGCATC TCTGGGCCCA ACCAGTGGCG AGACCAGCTC
    CGTCCCTCCC AACTGCTCCA CCTCTTCTCC CTGCAGCACC ACTACAAGGC TCCCACCTAC
    ACGTCCGACC GGCTCATCTT CCGGGAGCAG GAGTACATCC TCTCCGAGCT GGAGGATGGG
    AAACCCCCCA ACCCTCACCT GGGGCCGGCA GAGGAGCGGC TGGCCCTGCA TGCACTGCGG
    AAACAAGGGC TGGTGCCGGA GCACGTGGAG ACCCGGCCCC TCTACAGCCC CCTGCAGCCA
    GAGATCGAGC AGGGAAAGCT ACAGATGTGG GTGGACCTGT TTCCGAAATC CTTGGGTCAG
    CCTGGCCCCC CTTTCAACAT CACACCACGC AAAGCCAAGA GGTTCTACTT GCGCTGCATC
    ATCTGGAACA CCAGGGACGT CATCCTCGAT GACCTCAGCA TCACTGGGGA GAAGATGAGT
    GACATCTATG TCAAAGGCTG GCTGGTTGGC CACGAGGAGA ACAAGCAGAA GACAGACGTG
    CACTACAGGT CGATGGGAGG AGAAGGCAAC TTCAACTGGA GGTTCATCTT CCCCTTCGAC
    TATCTCCCTG CCGAGCAGAT GTGTTGCGTT GCAAAGAAGG AGCATTTCTG GAGCTTGGAT
    AAGACGGAAA ACAAGATTTC ACCGCAGCTG ATCTTCCAGA TCTGGGACAA TGACAAGTTC
    TCCTTCGATG ACTATTTGGG CTCCATCCAG ATGGATCTCA ACAGGATGCC CAAGCCTGCC
    AAGACTGCTG AGAAGTGCTC CTTAGAGCTC ATAGATGACA GCTTGTCTGC AAGCCGCTTT
    GTGTCCCTCT TTGAGCAGAA GACTGTCAAG GGCTGGTGGC CCTGCATTGC AGAGCAGGAT
    GAGAAGAAGA TCCTGGCAGG AAAACTGGAA ATGACTTTGG AAATCGTGGC TGAACACGAG
    CATGAAGAGC GGCCAGCTGG CGTGGGTCGG GATGAGCCAA ACATGAACCC CAAGCTGGAG
    GACCCCAAGC GCCCAGAGAC CTCCTTCCTG TGGTTCACCT CCCCGTACAA GACCCTGAAG
    TACATCCTGT GGCGCCGGTA CAAGTGGCTG CTCATCCTGG CCATCGTGCT CTTTGTTTTG
    CTGCTCTTCC TTGGGATCTT CATCTACGCC TTCCCGAACT ACGCTGCTAT GAAGCTGGTG
    AAACCTTTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150242
    Clone ID Related Accession (Same CDS sequence) XM_025149870.1
    Accession Version XM_025149870.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGCT GCGTGCTGCT GCGCGCCCAA CTGCGCCGCC CCGATAAGGG GGACCGGCAG 
    AGCGACCCCG TGGCCAGCCT GACCTTCCGA GGGGTGAAGA AGAAGACGAA GGTCATCAAG
    AACAGCATCA ACCCCGTATG GAACGAGGGC TTTGAATGGG ATCTGAAGGG CGTTGCTTTG
    GACCTGGGAT CTGAGCTCTG TGTTGTGGTT AAGGACCATG AGACTGTGGG GAGGAATCGG
    TTTTTGGGAG AGGCCCGCGT CCCCCTACGG GATGTGCTGT CCTCCCCCAG CCTGGCGGCC
    TCCTATGATG TCCCATTGCT GGATAGCAAG AAGCAGAGCA CCGGGGCTTC CCTCATCCTA
    CAAGTGTCCT ACAGCCCTCC TCCGGGCGCT GCCCCCCTCT TCCCTCCCCC AACCCCCCCC
    GAGGCCGCCC CGGCGGCGGT GGCAGAGTCC GACACTCTCA CTGGTAATGC GGGGGCTCCA
    CGGGGGGACG CGTCCCCCAT AGCCCACCCC ACAGCGGCCA GGGACCCGCA GCAACCTCCC
    TGCCTCCCGC CCCGCGCAGA AGTGGCCACC GAGGAGGAGG GGGAGGACCA GGCGGTGACG
    GGGGACGAGA CGGAGCCCCC ATCCTCGGGG CCGCCGGGCT CGGAGCCGCC CTCGGTGCCC
    CACAGACCCC CGGCTCCCCG CGGCCGTGGG GTGAAGCGCA GGAGGAGCTC GCCCAAAAAG
    CCGCTGTCCA ACAAACCTCA GGACTTCCAG ATCCGGGTTC GGGTGATCGA GGCCCGGCAG
    CTGCCCGGCG TTAACATCCG GCCCGTGGTG AAGGTGACGG CGGCGGGGCA GACCAAGCGG
    ACCAGGATAC GCAAAGGCAA CAGCCCCCTC TTCGACGAGA CCTTCTTCTT CAACGTCTTC
    GAGTCGCCAG CCGAGCTGTT CGACGTGCCC GTCTTCATCA CGGTCCTGGA CTCGCGCTCC
    TTCCGCATGG ATGCGGTGAT CGGAGAGTTT CGGATGGACG TTGAGAGCAT CTATTCGGAG
    CCGAAACACG CGATCCTCAG GCGGTGGCTG CTGCTCTCTG ACCCGGAGGA CTTCACGGCG
    GGGGCAAAGG GGTACCTGAA GGTCAGCCTG CTTGTGCTGG GCCCCGGGGA CGAAGCTCCT
    GTGGAGAAGA AGGACGTCTC TGAAGACAAG GAAGACATTG AGGGGAACCT CCTGCGACCC
    ACGGGGGTCA CCTTGCGAGG GGCTCAGTTC TGCCTGAAGA TCTTCAAGGC TGAGGATTTG
    CCCCAGATGG ACGACGCCGT CATGGACAAC GTCCGGCAGA TCTTCGGCTT CGAAAGCAAC
    AAGAAGAACC TGGTTGACCC CTTTGTGGAA GTCAGCTTTG CGGGGAAGAC GCTCTACTCG
    AGGATCCTGG AGAAGAATGC CAACCCGCAG TGGAATCAGT GCCTGATGCT GCCGGTGATG
    TTCCCTTCCA TGTGCGAGAA GATGAGGATC CGGGTGACCG ACTGGGACCG TCTCACACAC
    AACGACGTCG TCGGCACCGC CTACCTCTGC ATGTCCAAGA TCTCTGCGCC CGGCGGGGAG
    CTGGAGGATG CGTTTCCTGC TCTCACGAAG CCTCTGAAGG CCACAGACCT GGACGACGGG
    CTGGGCTTCC TGCCGACCTT CGGCCCCTGC TACGTCAACC TGTACGGCAG CCCCCGTGAG
    TTCACCGGCT TCCCGGATCC GTACGAGGCG CTCAATTTAG GAAAGGGCGA GGGCGTCGCG
    TACCGCGGCC GGGTGCTGCT GGAGCTGGAG ACCAAGCTGG CGGAGCACGG GGAGCTGAAG
    GTGGAGGACA TCTGTGCGGA TGACATCCTG CGGGTGGAGA AGTACCTGCG GCGGAGGAAG
    TACTCCCTCT TTGCTGCCTT CTACTCGGCC ACCATGCTGC AGGACGTGGA CGATGCCATC
    CAGTTTGAGG TCAGCATCGG CAACTACGGC AACAAGTTCG ACAACACCTG CTTGCCCTTG
    GCCTCCACCA CGCAGTACAG CCGGGCCGTG TTCGATGGTT GCCAGTATTA CTACCTGCCC
    TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCCTCCTATT GGGAAGATAT CAGCTACCGA
    ACCGACACTC AGAACCTCCT CCAGCACGCA GCAGACAGGC TGGAAGCCAA CCTGGAGCGG
    GTCCACCTCG CGCTCAAGGC CAACGGCGCG CCGGGCGAGC TGGAGTCCCT GGCTGCCCAG
    CTGATGGACG ACGTGATTGC AGACTGCAGT GTTCCGCTGC CCGACGTGCT GGGGAAGGCG
    GCCAGCACAC ACCTGGACCA GAACCTGTAC CGCTTCCGCA GCACCAACCT GGAGCAGATG
    GTGCGGGCGG CCCTGAGGCT GAAGCACGAG GAGCTGAGCC TGCCGGCCGT GCTGGAGCAG
    GCGGAGGACT GGCTGTGCCG GCTGCGTGCC ATGGCTGAGG AGCCCCAGAA CAGCCTCCCA
    GACGTGGTGA TCTGGATGCT GCGGGGTGAG AAGCGCGTTG CCTACGCCCG CGTGCCGGCC
    CGCGAGGTGC TCTACTCCCA AAGTGGCCCC AACTGCTGCG GTAGGAACTG TGGCAAGCTG
    CAGACCATCT TCCTGAAGTA CCCCCAGGAG GAGGCGATGG GGCCGCGCAT CCCAGCCCAG
    ATCCGCGTCC AGCTCTGGTT TGGGCTCTCT GTCAATGAGA AGGAGTTCAA CCAATACGCT
    GAGGGGAAGC TCTCCGTCTT TGCTGAAACC TACGAGAACC AGACCAAGCT GGCGCTGGTG
    GGGAACTGGG GCACCACCGG CCTGACTTAC CCCAAATACT CCGATGTCAC CGGCAAGATC
    AAGCTCCCCA AGGACAGCTT CCGGCCCTCC ACGGGCTGGA CCTGGGCTGG TGATTGGTTC
    ATCTGCCCAG AGAAGACGAT GCTGCACGAG GCAGACGCGG GGCACCTGAG CTTCGTGGAG
    GAGGTGTTTG AGAACCAGGT GCGGCTGCCG GGCGGGCAGT GGATCCACAT GTCCGATGCC
    TACACCGATG TGAATGGGGA GAAGGTTCTG CCCAAGGATG AGATCGAGTG TCCTCCGGGC
    TGGAAATGGG AAGACGTGGA GTGGGACACT GACCTCAACA GAGCCGTGGA TGAGAAAGGC
    TGGGAGTACG GCATCACCAT CCCTCCGGAC CGCCGGCCCA AGGCCTGGGT GCCAGCTGAG
    AAGATGTACC ACACCAACCG GCGCCGGCGC TGGGTGCGAC TGCGCAGCAG GGACCTGGCC
    CAGATGGAGA CTGTCAGGAA GCACAAGCAG GACGAGCAGG ACGGCGAGGG CTGGGAATAC
    GCCTCGCTGC TGGGCTGGCG CTTCCACCTG CGGCCGCGTC GCACCGATGC CTTCCGCCGC
    CGCCGTTGGA GACGTAGGAT GGAGCCCTTG GAGCGCACCG GGGCTGCTGC TGTGTTCGCC
    TTGGAGGGGG CCCTGGGAGG AATGACGGAC GACAAAAGCG ACGATGGCAA ATCCGTCTCC
    ACGCTGAGTT TCGGCGTCAA CAGACCCACC ATCTCCTGCA TCTTCGACTA TGGGAACCGC
    TACCACCTGC GCTGCTACAT GTACCAGGCA CGGGACCTGG CTGCCATGGA CAAGGACAGC
    TTCTCGGACC CCTATGCCAT CGTTTCCTTC CTCCACCAAA GCCAGAAGAC GGTGGTGATC
    AAGAACACCC TGAACCCCAC GTGGGACCAG ACGCTCATCT TCTACGAGAT AGAGATCTTC
    GGAGACCCCA AGAACGTGTC TGACTGCCCT CCCAGCATCG TGGTGGAGAT ATACGACCAC
    GACACCTACG GCGCTGATGA GTTCATGGGG CGCTGCGTGT GCCGGCCCAG CCTGCAGCGC
    GCGCCGCGCC TCTCCTGGCA CCCCGTGCTC AAAGGCGGCA GGAACGTCGG CGAGCTGCTG
    GCTGCCTTCG AGCTCATCCA GAGGGAGAAG CCGGCTGTCC ACCACATCCC TGGCTTCGAG
    AACGAGCTGT CCTCCGCTCT GGATGAGCTC TGTGCCCCTG CCTTGTTCTA CGAGGGGCTC
    CTCCGATGGT CTGAGGACTC TGACCTGCCC TACCCACCGC CCCAACGGGA ACCCAACATC
    TTCATGGTCC CCCAAGGGAT CAAACCGGTC CTGCAGCGCA CGGCCATCGA GATCCTGGCC
    TGGGGGCTGA GGAACCTCAA GAGCTACCAG CTGGCCAGCG TCACCTCACC CAGCCTGCTG
    GTGGAGTGCG GGGGGCAGCT CGTGCAGTCC TGTGTCATCA AGAACGTCAA GAAAAACCCC
    AACTTTGATG TCTGTGTGCT CTTCATGGAA GTGCGTCTGC CCAGCGAGAG CCTCTACAGT
    CCCCCCATCA TCATCAAAAT CATCGACAAC CGTCCGTTCG GCCGCAGGCC CGTGGTGGGG
    CAGTGCACCA TCCGCTCACT GGAGGAATTC TACTGTGACC CCTATGGTGA GGAGACGGAT
    GGTGCCCAGG AGCAGGCAGA TGATGTGAGC CTCACGCCCA GGGATGATGT CCTCATTGAC
    ATTGATGACA AAGAGCCTCT CCTTCCCACC CAGCTTGTGG AGGGCGTGAC CAGCTCAGCG
    CTAATTAACC CGCCAGCTTC TAGGTCCGAG CCTCAAGAGG AAGAATTCAT TGACTGGTGG
    AGCAAATTCT ACGCTTCCAC AGGAGAGAGG GAGAAGTGTG GCTCCTACTT GGAGAAGGGC
    TTTGACACCC TGCAGGTCTA TGAGACGGAG CTGGAGCGCG TGGAGGCCTT CGAGCAGCTC
    TCTGACTTCT GCCACACCTT CAGGCTGTAC CGTGGCCGGG TGCAGGATGC CAACGATGAT
    CCCTCAGTTG TGGGCGAGTT CAAGGGCTCC TTCAAAATCT ACCCTCTGCC CGATGACCCA
    CGCGTGCCGC TGCCGCCGCG GCAGTTCCAC CAACTGCCAG CCAGGGGTCC CCAGGAGTGT
    CTCGTCAGGG TCTACATCAT CCGTGCCTTC GGTCTGCAGC CCAAGGATGC CAATGGGAAG
    TGCGATCCGT ATGTGAAGAT TTCAGTGGGA AAGAAATCCA TAAACGACCA AGAAAATTAC
    CTCCCCTCTA CGCTGGAGCC TGTCTTTGGA AAGATGTTTG AGTTGAGCTG CACTCTGCCC
    CTGGAGAAGG ACCTGAAGGT CACACTCTAT GACTACGACC TCCTGTCCAA GGATGAGAAG
    ATTGGGGAGA CCGTCATCGA CCTGGAGAAT CGGTTCCTGT CGAAATATGG GGCGCGCTGT
    GGGCTTCCCC AGACCTACTG CATCTCTGGG CCCAACCAGT GGCGAGACCA GCTCCGTCCC
    TCCCAACTGC TCCACCTCTT CTCCCTGCAG CACCACTACA AGGCTCCCAC CTACACGTCC
    GACCGGCTCA TCTTCCGGGA GCAGGAGTAC ATCCTCTCCG AGCTGGAGGA TGGGAAACCC
    CCCAACCCTC ACCTGGGGCC GGCAGAGGAG CGGCTGGCCC TGCATGCACT GCGGAAACAA
    GGGCTGGTGC CGGAGCACGT GGAGACCCGG CCCCTCTACA GCCCCCTGCA GCCAGAGATC
    GAGCAGGGAA AGCTACAGAT GTGGGTGGAC CTGTTTCCGA AATCCTTGGG TCAGCCTGGC
    CCCCCTTTCA ACATCACACC ACGCAAAGCC AAGAGGTTCT ACTTGCGCTG CATCATCTGG
    AACACCAGGG ACGTCATCCT CGATGACCTC AGCATCACTG GGGAGAAGAT GAGTGACATC
    TATGTCAAAG GCTGGCTGGT TGGCCACGAG GAGAACAAGC AGAAGACAGA CGTGCACTAC
    AGGTCGATGG GAGGAGAAGG CAACTTCAAC TGGAGGTTCA TCTTCCCCTT CGACTATCTC
    CCTGCCGAGC AGATGTGTTG CGTTGCAAAG AAGGAGCATT TCTGGAGCTT GGATAAGACG
    GAAAACAAGA TTTCACCGCA GCTGATCTTC CAGATCTGGG ACAATGACAA GTTCTCCTTC
    GATGACTATT TGGGCTCCAT CCAGATGGAT CTCAACAGGA TGCCCAAGCC TGCCAAGACT
    GCTGAGAAGT GCTCCTTAGA GCTCATAGAT GACAGCTTGT CTGCAAGCCG CTTTGTGTCC
    CTCTTTGAGC AGAAGACTGT CAAGGGCTGG TGGCCCTGCA TTGCAGAGCA GGATGAGAAG
    AAGATCCTGG CAGGAAAACT GGAAATGACT TTGGAAATCG TGGCTGAACA CGAGCATGAA
    GAGCGGCCAG CTGGCGTGGG TCGGGATGAG CCAAACATGA ACCCCAAGCT GGAGGACCCC
    AAGCGCCCAG AGACCTCCTT CCTGTGGTTC ACCTCCCCGT ACAAGACCCT GAAGTACATC
    CTGTGGCGCC GGTACAAGTG GCTGCTCATC CTGGCCATCG TGCTCTTTGT TTTGCTGCTC
    TTCCTTGGGA TCTTCATCTA CGCCTTCCCG AACTACGCTG CTATGAAGCT GGTGAAACCT
    TTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005638.1
    CDS36..6464
    Translation

    Target ORF information:

    RefSeq Version XM_025149870.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149870.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGCT GCGTGCTGCT GCGCGCCCAA CTGCGCCGCC CCGATAAGGG GGACCGGCAG 
    AGCGACCCCG TGGCCAGCCT GACCTTCCGA GGGGTGAAGA AGAAGACGAA GGTCATCAAG
    AACAGCATCA ACCCCGTATG GAACGAGGGC TTTGAATGGG ATCTGAAGGG CGTTGCTTTG
    GACCTGGGAT CTGAGCTCTG TGTTGTGGTT AAGGACCATG AGACTGTGGG GAGGAATCGG
    TTTTTGGGAG AGGCCCGCGT CCCCCTACGG GATGTGCTGT CCTCCCCCAG CCTGGCGGCC
    TCCTATGATG TCCCATTGCT GGATAGCAAG AAGCAGAGCA CCGGGGCTTC CCTCATCCTA
    CAAGTGTCCT ACAGCCCTCC TCCGGGCGCT GCCCCCCTCT TCCCTCCCCC AACCCCCCCC
    GAGGCCGCCC CGGCGGCGGT GGCAGAGTCC GACACTCTCA CTGGTAATGC GGGGGCTCCA
    CGGGGGGACG CGTCCCCCAT AGCCCACCCC ACAGCGGCCA GGGACCCGCA GCAACCTCCC
    TGCCTCCCGC CCCGCGCAGA AGTGGCCACC GAGGAGGAGG GGGAGGACCA GGCGGTGACG
    GGGGACGAGA CGGAGCCCCC ATCCTCGGGG CCGCCGGGCT CGGAGCCGCC CTCGGTGCCC
    CACAGACCCC CGGCTCCCCG CGGCCGTGGG GTGAAGCGCA GGAGGAGCTC GCCCAAAAAG
    CCGCTGTCCA ACAAACCTCA GGACTTCCAG ATCCGGGTTC GGGTGATCGA GGCCCGGCAG
    CTGCCCGGCG TTAACATCCG GCCCGTGGTG AAGGTGACGG CGGCGGGGCA GACCAAGCGG
    ACCAGGATAC GCAAAGGCAA CAGCCCCCTC TTCGACGAGA CCTTCTTCTT CAACGTCTTC
    GAGTCGCCAG CCGAGCTGTT CGACGTGCCC GTCTTCATCA CGGTCCTGGA CTCGCGCTCC
    TTCCGCATGG ATGCGGTGAT CGGAGAGTTT CGGATGGACG TTGAGAGCAT CTATTCGGAG
    CCGAAACACG CGATCCTCAG GCGGTGGCTG CTGCTCTCTG ACCCGGAGGA CTTCACGGCG
    GGGGCAAAGG GGTACCTGAA GGTCAGCCTG CTTGTGCTGG GCCCCGGGGA CGAAGCTCCT
    GTGGAGAAGA AGGACGTCTC TGAAGACAAG GAAGACATTG AGGGGAACCT CCTGCGACCC
    ACGGGGGTCA CCTTGCGAGG GGCTCAGTTC TGCCTGAAGA TCTTCAAGGC TGAGGATTTG
    CCCCAGATGG ACGACGCCGT CATGGACAAC GTCCGGCAGA TCTTCGGCTT CGAAAGCAAC
    AAGAAGAACC TGGTTGACCC CTTTGTGGAA GTCAGCTTTG CGGGGAAGAC GCTCTACTCG
    AGGATCCTGG AGAAGAATGC CAACCCGCAG TGGAATCAGT GCCTGATGCT GCCGGTGATG
    TTCCCTTCCA TGTGCGAGAA GATGAGGATC CGGGTGACCG ACTGGGACCG TCTCACACAC
    AACGACGTCG TCGGCACCGC CTACCTCTGC ATGTCCAAGA TCTCTGCGCC CGGCGGGGAG
    CTGGAGGATG CGTTTCCTGC TCTCACGAAG CCTCTGAAGG CCACAGACCT GGACGACGGG
    CTGGGCTTCC TGCCGACCTT CGGCCCCTGC TACGTCAACC TGTACGGCAG CCCCCGTGAG
    TTCACCGGCT TCCCGGATCC GTACGAGGCG CTCAATTTAG GAAAGGGCGA GGGCGTCGCG
    TACCGCGGCC GGGTGCTGCT GGAGCTGGAG ACCAAGCTGG CGGAGCACGG GGAGCTGAAG
    GTGGAGGACA TCTGTGCGGA TGACATCCTG CGGGTGGAGA AGTACCTGCG GCGGAGGAAG
    TACTCCCTCT TTGCTGCCTT CTACTCGGCC ACCATGCTGC AGGACGTGGA CGATGCCATC
    CAGTTTGAGG TCAGCATCGG CAACTACGGC AACAAGTTCG ACAACACCTG CTTGCCCTTG
    GCCTCCACCA CGCAGTACAG CCGGGCCGTG TTCGATGGTT GCCAGTATTA CTACCTGCCC
    TGGGGCAACG TGAAGCCCGT GGTGGTGCTG TCCTCCTATT GGGAAGATAT CAGCTACCGA
    ACCGACACTC AGAACCTCCT CCAGCACGCA GCAGACAGGC TGGAAGCCAA CCTGGAGCGG
    GTCCACCTCG CGCTCAAGGC CAACGGCGCG CCGGGCGAGC TGGAGTCCCT GGCTGCCCAG
    CTGATGGACG ACGTGATTGC AGACTGCAGT GTTCCGCTGC CCGACGTGCT GGGGAAGGCG
    GCCAGCACAC ACCTGGACCA GAACCTGTAC CGCTTCCGCA GCACCAACCT GGAGCAGATG
    GTGCGGGCGG CCCTGAGGCT GAAGCACGAG GAGCTGAGCC TGCCGGCCGT GCTGGAGCAG
    GCGGAGGACT GGCTGTGCCG GCTGCGTGCC ATGGCTGAGG AGCCCCAGAA CAGCCTCCCA
    GACGTGGTGA TCTGGATGCT GCGGGGTGAG AAGCGCGTTG CCTACGCCCG CGTGCCGGCC
    CGCGAGGTGC TCTACTCCCA AAGTGGCCCC AACTGCTGCG GTAGGAACTG TGGCAAGCTG
    CAGACCATCT TCCTGAAGTA CCCCCAGGAG GAGGCGATGG GGCCGCGCAT CCCAGCCCAG
    ATCCGCGTCC AGCTCTGGTT TGGGCTCTCT GTCAATGAGA AGGAGTTCAA CCAATACGCT
    GAGGGGAAGC TCTCCGTCTT TGCTGAAACC TACGAGAACC AGACCAAGCT GGCGCTGGTG
    GGGAACTGGG GCACCACCGG CCTGACTTAC CCCAAATACT CCGATGTCAC CGGCAAGATC
    AAGCTCCCCA AGGACAGCTT CCGGCCCTCC ACGGGCTGGA CCTGGGCTGG TGATTGGTTC
    ATCTGCCCAG AGAAGACGAT GCTGCACGAG GCAGACGCGG GGCACCTGAG CTTCGTGGAG
    GAGGTGTTTG AGAACCAGGT GCGGCTGCCG GGCGGGCAGT GGATCCACAT GTCCGATGCC
    TACACCGATG TGAATGGGGA GAAGGTTCTG CCCAAGGATG AGATCGAGTG TCCTCCGGGC
    TGGAAATGGG AAGACGTGGA GTGGGACACT GACCTCAACA GAGCCGTGGA TGAGAAAGGC
    TGGGAGTACG GCATCACCAT CCCTCCGGAC CGCCGGCCCA AGGCCTGGGT GCCAGCTGAG
    AAGATGTACC ACACCAACCG GCGCCGGCGC TGGGTGCGAC TGCGCAGCAG GGACCTGGCC
    CAGATGGAGA CTGTCAGGAA GCACAAGCAG GACGAGCAGG ACGGCGAGGG CTGGGAATAC
    GCCTCGCTGC TGGGCTGGCG CTTCCACCTG CGGCCGCGTC GCACCGATGC CTTCCGCCGC
    CGCCGTTGGA GACGTAGGAT GGAGCCCTTG GAGCGCACCG GGGCTGCTGC TGTGTTCGCC
    TTGGAGGGGG CCCTGGGAGG AATGACGGAC GACAAAAGCG ACGATGGCAA ATCCGTCTCC
    ACGCTGAGTT TCGGCGTCAA CAGACCCACC ATCTCCTGCA TCTTCGACTA TGGGAACCGC
    TACCACCTGC GCTGCTACAT GTACCAGGCA CGGGACCTGG CTGCCATGGA CAAGGACAGC
    TTCTCGGACC CCTATGCCAT CGTTTCCTTC CTCCACCAAA GCCAGAAGAC GGTGGTGATC
    AAGAACACCC TGAACCCCAC GTGGGACCAG ACGCTCATCT TCTACGAGAT AGAGATCTTC
    GGAGACCCCA AGAACGTGTC TGACTGCCCT CCCAGCATCG TGGTGGAGAT ATACGACCAC
    GACACCTACG GCGCTGATGA GTTCATGGGG CGCTGCGTGT GCCGGCCCAG CCTGCAGCGC
    GCGCCGCGCC TCTCCTGGCA CCCCGTGCTC AAAGGCGGCA GGAACGTCGG CGAGCTGCTG
    GCTGCCTTCG AGCTCATCCA GAGGGAGAAG CCGGCTGTCC ACCACATCCC TGGCTTCGAG
    AACGAGCTGT CCTCCGCTCT GGATGAGCTC TGTGCCCCTG CCTTGTTCTA CGAGGGGCTC
    CTCCGATGGT CTGAGGACTC TGACCTGCCC TACCCACCGC CCCAACGGGA ACCCAACATC
    TTCATGGTCC CCCAAGGGAT CAAACCGGTC CTGCAGCGCA CGGCCATCGA GATCCTGGCC
    TGGGGGCTGA GGAACCTCAA GAGCTACCAG CTGGCCAGCG TCACCTCACC CAGCCTGCTG
    GTGGAGTGCG GGGGGCAGCT CGTGCAGTCC TGTGTCATCA AGAACGTCAA GAAAAACCCC
    AACTTTGATG TCTGTGTGCT CTTCATGGAA GTGCGTCTGC CCAGCGAGAG CCTCTACAGT
    CCCCCCATCA TCATCAAAAT CATCGACAAC CGTCCGTTCG GCCGCAGGCC CGTGGTGGGG
    CAGTGCACCA TCCGCTCACT GGAGGAATTC TACTGTGACC CCTATGGTGA GGAGACGGAT
    GGTGCCCAGG AGCAGGCAGA TGATGTGAGC CTCACGCCCA GGGATGATGT CCTCATTGAC
    ATTGATGACA AAGAGCCTCT CCTTCCCACC CAGCTTGTGG AGGGCGTGAC CAGCTCAGCG
    CTAATTAACC CGCCAGCTTC TAGGTCCGAG CCTCAAGAGG AAGAATTCAT TGACTGGTGG
    AGCAAATTCT ACGCTTCCAC AGGAGAGAGG GAGAAGTGTG GCTCCTACTT GGAGAAGGGC
    TTTGACACCC TGCAGGTCTA TGAGACGGAG CTGGAGCGCG TGGAGGCCTT CGAGCAGCTC
    TCTGACTTCT GCCACACCTT CAGGCTGTAC CGTGGCCGGG TGCAGGATGC CAACGATGAT
    CCCTCAGTTG TGGGCGAGTT CAAGGGCTCC TTCAAAATCT ACCCTCTGCC CGATGACCCA
    CGCGTGCCGC TGCCGCCGCG GCAGTTCCAC CAACTGCCAG CCAGGGGTCC CCAGGAGTGT
    CTCGTCAGGG TCTACATCAT CCGTGCCTTC GGTCTGCAGC CCAAGGATGC CAATGGGAAG
    TGCGATCCGT ATGTGAAGAT TTCAGTGGGA AAGAAATCCA TAAACGACCA AGAAAATTAC
    CTCCCCTCTA CGCTGGAGCC TGTCTTTGGA AAGATGTTTG AGTTGAGCTG CACTCTGCCC
    CTGGAGAAGG ACCTGAAGGT CACACTCTAT GACTACGACC TCCTGTCCAA GGATGAGAAG
    ATTGGGGAGA CCGTCATCGA CCTGGAGAAT CGGTTCCTGT CGAAATATGG GGCGCGCTGT
    GGGCTTCCCC AGACCTACTG CATCTCTGGG CCCAACCAGT GGCGAGACCA GCTCCGTCCC
    TCCCAACTGC TCCACCTCTT CTCCCTGCAG CACCACTACA AGGCTCCCAC CTACACGTCC
    GACCGGCTCA TCTTCCGGGA GCAGGAGTAC ATCCTCTCCG AGCTGGAGGA TGGGAAACCC
    CCCAACCCTC ACCTGGGGCC GGCAGAGGAG CGGCTGGCCC TGCATGCACT GCGGAAACAA
    GGGCTGGTGC CGGAGCACGT GGAGACCCGG CCCCTCTACA GCCCCCTGCA GCCAGAGATC
    GAGCAGGGAA AGCTACAGAT GTGGGTGGAC CTGTTTCCGA AATCCTTGGG TCAGCCTGGC
    CCCCCTTTCA ACATCACACC ACGCAAAGCC AAGAGGTTCT ACTTGCGCTG CATCATCTGG
    AACACCAGGG ACGTCATCCT CGATGACCTC AGCATCACTG GGGAGAAGAT GAGTGACATC
    TATGTCAAAG GCTGGCTGGT TGGCCACGAG GAGAACAAGC AGAAGACAGA CGTGCACTAC
    AGGTCGATGG GAGGAGAAGG CAACTTCAAC TGGAGGTTCA TCTTCCCCTT CGACTATCTC
    CCTGCCGAGC AGATGTGTTG CGTTGCAAAG AAGGAGCATT TCTGGAGCTT GGATAAGACG
    GAAAACAAGA TTTCACCGCA GCTGATCTTC CAGATCTGGG ACAATGACAA GTTCTCCTTC
    GATGACTATT TGGGCTCCAT CCAGATGGAT CTCAACAGGA TGCCCAAGCC TGCCAAGACT
    GCTGAGAAGT GCTCCTTAGA GCTCATAGAT GACAGCTTGT CTGCAAGCCG CTTTGTGTCC
    CTCTTTGAGC AGAAGACTGT CAAGGGCTGG TGGCCCTGCA TTGCAGAGCA GGATGAGAAG
    AAGATCCTGG CAGGAAAACT GGAAATGACT TTGGAAATCG TGGCTGAACA CGAGCATGAA
    GAGCGGCCAG CTGGCGTGGG TCGGGATGAG CCAAACATGA ACCCCAAGCT GGAGGACCCC
    AAGCGCCCAG AGACCTCCTT CCTGTGGTTC ACCTCCCCGT ACAAGACCCT GAAGTACATC
    CTGTGGCGCC GGTACAAGTG GCTGCTCATC CTGGCCATCG TGCTCTTTGT TTTGCTGCTC
    TTCCTTGGGA TCTTCATCTA CGCCTTCCCG AACTACGCTG CTATGAAGCT GGTGAAACCT
    TTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150243
    Clone ID Related Accession (Same CDS sequence) XM_025149871.1
    Accession Version XM_025149871.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGTGGA CGACGGGCTG GGCTTCCTGC CGACCTTCGG CCCCTGCTAC
    GTCAACCTGT ACGGCAGCCC CCGTGAGTTC ACCGGCTTCC CGGATCCGTA CGAGGCGCTC
    AATTTAGGAA AGGGCGAGGG CGTCGCGTAC CGCGGCCGGG TGCTGCTGGA GCTGGAGACC
    AAGCTGGCGG AGCACGGGGA GCTGAAGGTG GAGGACATCT GTGCGGATGA CATCCTGCGG
    GTGGAGAAGT ACCTGCGGCG GAGGAAGTAC TCCCTCTTTG CTGCCTTCTA CTCGGCCACC
    ATGCTGCAGG ACGTGGACGA TGCCATCCAG TTTGAGGTCA GCATCGGCAA CTACGGCAAC
    AAGTTCGACA ACACCTGCTT GCCCTTGGCC TCCACCACGC AGTACAGCCG GGCCGTGTTC
    GATGGTTGCC AGTATTACTA CCTGCCCTGG GGCAACGTGA AGCCCGTGGT GGTGCTGTCC
    TCCTATTGGG AAGATATCAG CTACCGAACC GACACTCAGA ACCTCCTCCA GCACGCAGCA
    GACAGGCTGG AAGCCAACCT GGAGCGGGTC CACCTCGCGC TCAAGGCCAA CGGCGCGCCG
    GGCGAGCTGG AGTCCCTGGC TGCCCAGCTG ATGGACGACG TGATTGCAGA CTGCAGTGTT
    CCGCTGCCCG ACGTGCTGGG GAAGGCGGCC AGCACACACC TGGACCAGAA CCTGTACCGC
    TTCCGCAGCA CCAACCTGGA GCAGATGGTG CGGGCGGCCC TGAGGCTGAA GCACGAGGAG
    CTGAGCCTGC CGGCCGTGCT GGAGCAGGCG GAGGACTGGC TGTGCCGGCT GCGTGCCATG
    GCTGAGGAGC CCCAGAACAG CCTCCCAGAC GTGGTGATCT GGATGCTGCG GGGTGAGAAG
    CGCGTTGCCT ACGCCCGCGT GCCGGCCCGC GAGGTGCTCT ACTCCCAAAG TGGCCCCAAC
    TGCTGCGGTA GGAACTGTGG CAAGCTGCAG ACCATCTTCC TGAAGTACCC CCAGGAGGAG
    GCGATGGGGC CGCGCATCCC AGCCCAGATC CGCGTCCAGC TCTGGTTTGG GCTCTCTGTC
    AATGAGAAGG AGTTCAACCA ATACGCTGAG GGGAAGCTCT CCGTCTTTGC TGAAACCTAC
    GAGAACCAGA CCAAGCTGGC GCTGGTGGGG AACTGGGGCA CCACCGGCCT GACTTACCCC
    AAATACTCCG ATGTCACCGG CAAGATCAAG CTCCCCAAGG ACAGCTTCCG GCCCTCCACG
    GGCTGGACCT GGGCTGGTGA TTGGTTCATC TGCCCAGAGA AGACGATGCT GCACGAGGCA
    GACGCGGGGC ACCTGAGCTT CGTGGAGGAG GTGTTTGAGA ACCAGGTGCG GCTGCCGGGC
    GGGCAGTGGA TCCACATGTC CGATGCCTAC ACCGATGTGA ATGGGGAGAA GGTTCTGCCC
    AAGGATGAGA TCGAGTGTCC TCCGGGCTGG AAATGGGAAG ACGTGGAGTG GGACACTGAC
    CTCAACAGAG CCGTGGATGA GAAAGGCTGG GAGTACGGCA TCACCATCCC TCCGGACCGC
    CGGCCCAAGG CCTGGGTGCC AGCTGAGAAG ATGTACCACA CCAACCGGCG CCGGCGCTGG
    GTGCGACTGC GCAGCAGGGA CCTGGCCCAG ATGGAGACTG TCAGGAAGCA CAAGCAGGAC
    GAGCAGGACG GCGAGGGCTG GGAATACGCC TCGCTGCTGG GCTGGCGCTT CCACCTGCGG
    CCGCGTCGCA CCGATGCCTT CCGCCGCCGC CGTTGGAGAC GTAGGATGGA GCCCTTGGAG
    CGCACCGGGG CTGCTGCTGT GTTCGCCTTG GAGGGGGCCC TGGGAGGAAT GACGGACGAC
    AAAAGCGACG ATGGCAAATC CGTCTCCACG CTGAGTTTCG GCGTCAACAG ACCCACCATC
    TCCTGCATCT TCGACTATGG GAACCGCTAC CACCTGCGCT GCTACATGTA CCAGGCACGG
    GACCTGGCTG CCATGGACAA GGACAGCTTC TCGGACCCCT ATGCCATCGT TTCCTTCCTC
    CACCAAAGCC AGAAGACGGT GGTGATCAAG AACACCCTGA ACCCCACGTG GGACCAGACG
    CTCATCTTCT ACGAGATAGA GATCTTCGGA GACCCCAAGA ACGTGTCTGA CTGCCCTCCC
    AGCATCGTGG TGGAGATATA CGACCACGAC ACCTACGGCG CTGATGAGTT CATGGGGCGC
    TGCGTGTGCC GGCCCAGCCT GCAGCGCGCG CCGCGCCTCT CCTGGCACCC CGTGCTCAAA
    GGCGGCAGGA ACGTCGGCGA GCTGCTGGCT GCCTTCGAGC TCATCCAGAG GGAGAAGCCG
    GCTGTCCACC ACATCCCTGG CTTCGAGAAC GAGCTGTCCT CCGCTCTGGA TGAGTCTGAG
    GACTCTGACC TGCCCTACCC ACCGCCCCAA CGGGAACCCA ACATCTTCAT GGTCCCCCAA
    GGGATCAAAC CGGTCCTGCA GCGCACGGCC ATCGAGATCC TGGCCTGGGG GCTGAGGAAC
    CTCAAGAGCT ACCAGCTGGC CAGCGTCACC TCACCCAGCC TGCTGGTGGA GTGCGGGGGG
    CAGCTCGTGC AGTCCTGTGT CATCAAGAAC GTCAAGAAAA ACCCCAACTT TGATGTCTGT
    GTGCTCTTCA TGGAAGTGCG TCTGCCCAGC GAGAGCCTCT ACAGTCCCCC CATCATCATC
    AAAATCATCG ACAACCGTCC GTTCGGCCGC AGGCCCGTGG TGGGGCAGTG CACCATCCGC
    TCACTGGAGG AATTCTACTG TGACCCCTAT GGTGAGGAGA CGGATGGTGC CCAGGAGCAG
    GCAGATGATG TGAGCCTCAC GCCCAGGGAT GATGTCCTCA TTGACATTGA TGACAAAGAG
    CCTCTCCTTC CCACCCAGCT TGTGGAGGGC GTGACCAGCT CAGCGCTAAT TAACCCGCCA
    GCTTCTAGGT CCGAGCCTCA AGAGGAAGAA TTCATTGACT GGTGGAGCAA ATTCTACGCT
    TCCACAGGAG AGAGGGAGAA GTGTGGCTCC TACTTGGAGA AGGGCTTTGA CACCCTGCAG
    GTCTATGAGA CGGAGCTGGA GCGCGTGGAG GCCTTCGAGC AGCTCTCTGA CTTCTGCCAC
    ACCTTCAGGC TGTACCGTGG CCGGGTGCAG GATGCCAACG ATGATCCCTC AGTTGTGGGC
    GAGTTCAAGG GCTCCTTCAA AATCTACCCT CTGCCCGATG ACCCACGCGT GCCGCTGCCG
    CCGCGGCAGT TCCACCAACT GCCAGCCAGG GGTCCCCAGG AGTGTCTCGT CAGGGTCTAC
    ATCATCCGTG CCTTCGGTCT GCAGCCCAAG GATGCCAATG GGAAGTGCGA TCCGTATGTG
    AAGATTTCAG TGGGAAAGAA ATCCATAAAC GACCAAGAAA ATTACCTCCC CTCTACGCTG
    GAGCCTGTCT TTGGAAAGAT GTTTGAGTTG AGCTGCACTC TGCCCCTGGA GAAGGACCTG
    AAGGTCACAC TCTATGACTA CGACCTCCTG TCCAAGGATG AGAAGATTGG GGAGACCGTC
    ATCGACCTGG AGAATCGGTT CCTGTCGAAA TATGGGGCGC GCTGTGGGCT TCCCCAGACC
    TACTGCATCT CTGGGCCCAA CCAGTGGCGA GACCAGCTCC GTCCCTCCCA ACTGCTCCAC
    CTCTTCTCCC TGCAGCACCA CTACAAGGCT CCCACCTACA CGTCCGACCG GCTCATCTTC
    CGGGAGCAGG AGTACATCCT CTCCGAGCTG GAGGATGGGA AACCCCCCAA CCCTCACCTG
    GGGCCGGCAG AGGAGCGGCT GGCCCTGCAT GCACTGCGGA AACAAGGGCT GGTGCCGGAG
    CACGTGGAGA CCCGGCCCCT CTACAGCCCC CTGCAGCCAG AGATCGAGCA GGGAAAGCTA
    CAGATGTGGG TGGACCTGTT TCCGAAATCC TTGGGTCAGC CTGGCCCCCC TTTCAACATC
    ACACCACGCA AAGCCAAGAG GTTCTACTTG CGCTGCATCA TCTGGAACAC CAGGGACGTC
    ATCCTCGATG ACCTCAGCAT CACTGGGGAG AAGATGAGTG ACATCTATGT CAAAGGCTGG
    CTGGTTGGCC ACGAGGAGAA CAAGCAGAAG ACAGACGTGC ACTACAGGTC GATGGGAGGA
    GAAGGCAACT TCAACTGGAG GTTCATCTTC CCCTTCGACT ATCTCCCTGC CGAGCAGATG
    TGTTGCGTTG CAAAGAAGGA GCATTTCTGG AGCTTGGATA AGACGGAAAA CAAGATTTCA
    CCGCAGCTGA TCTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTCGATGA CTATTTGGGC
    TCCATCCAGA TGGATCTCAA CAGGATGCCC AAGCCTGCCA AGACTGCTGA GAAGTGCTCC
    TTAGAGCTCA TAGATGACAG CTTGTCTGCA AGCCGCTTTG TGTCCCTCTT TGAGCAGAAG
    ACTGTCAAGG GCTGGTGGCC CTGCATTGCA GAGCAGGATG AGAAGAAGAT CCTGGCAGGA
    AAACTGGAAA TGACTTTGGA AATCGTGGCT GAACACGAGC ATGAAGAGCG GCCAGCTGGC
    GTGGGTCGGG ATGAGCCAAA CATGAACCCC AAGCTGGAGG ACCCCAAGCG CCCAGAGACC
    TCCTTCCTGT GGTTCACCTC CCCGTACAAG ACCCTGAAGT ACATCCTGTG GCGCCGGTAC
    AAGTGGCTGC TCATCCTGGC CATCGTGCTC TTTGTTTTGC TGCTCTTCCT TGGGATCTTC
    ATCTACGCCT TCCCGAACTA CGCTGCTATG AAGCTGGTGA AACCTTTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005639.1
    CDS647..7060
    Translation

    Target ORF information:

    RefSeq Version XM_025149871.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149871.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGTGGA CGACGGGCTG GGCTTCCTGC CGACCTTCGG CCCCTGCTAC
    GTCAACCTGT ACGGCAGCCC CCGTGAGTTC ACCGGCTTCC CGGATCCGTA CGAGGCGCTC
    AATTTAGGAA AGGGCGAGGG CGTCGCGTAC CGCGGCCGGG TGCTGCTGGA GCTGGAGACC
    AAGCTGGCGG AGCACGGGGA GCTGAAGGTG GAGGACATCT GTGCGGATGA CATCCTGCGG
    GTGGAGAAGT ACCTGCGGCG GAGGAAGTAC TCCCTCTTTG CTGCCTTCTA CTCGGCCACC
    ATGCTGCAGG ACGTGGACGA TGCCATCCAG TTTGAGGTCA GCATCGGCAA CTACGGCAAC
    AAGTTCGACA ACACCTGCTT GCCCTTGGCC TCCACCACGC AGTACAGCCG GGCCGTGTTC
    GATGGTTGCC AGTATTACTA CCTGCCCTGG GGCAACGTGA AGCCCGTGGT GGTGCTGTCC
    TCCTATTGGG AAGATATCAG CTACCGAACC GACACTCAGA ACCTCCTCCA GCACGCAGCA
    GACAGGCTGG AAGCCAACCT GGAGCGGGTC CACCTCGCGC TCAAGGCCAA CGGCGCGCCG
    GGCGAGCTGG AGTCCCTGGC TGCCCAGCTG ATGGACGACG TGATTGCAGA CTGCAGTGTT
    CCGCTGCCCG ACGTGCTGGG GAAGGCGGCC AGCACACACC TGGACCAGAA CCTGTACCGC
    TTCCGCAGCA CCAACCTGGA GCAGATGGTG CGGGCGGCCC TGAGGCTGAA GCACGAGGAG
    CTGAGCCTGC CGGCCGTGCT GGAGCAGGCG GAGGACTGGC TGTGCCGGCT GCGTGCCATG
    GCTGAGGAGC CCCAGAACAG CCTCCCAGAC GTGGTGATCT GGATGCTGCG GGGTGAGAAG
    CGCGTTGCCT ACGCCCGCGT GCCGGCCCGC GAGGTGCTCT ACTCCCAAAG TGGCCCCAAC
    TGCTGCGGTA GGAACTGTGG CAAGCTGCAG ACCATCTTCC TGAAGTACCC CCAGGAGGAG
    GCGATGGGGC CGCGCATCCC AGCCCAGATC CGCGTCCAGC TCTGGTTTGG GCTCTCTGTC
    AATGAGAAGG AGTTCAACCA ATACGCTGAG GGGAAGCTCT CCGTCTTTGC TGAAACCTAC
    GAGAACCAGA CCAAGCTGGC GCTGGTGGGG AACTGGGGCA CCACCGGCCT GACTTACCCC
    AAATACTCCG ATGTCACCGG CAAGATCAAG CTCCCCAAGG ACAGCTTCCG GCCCTCCACG
    GGCTGGACCT GGGCTGGTGA TTGGTTCATC TGCCCAGAGA AGACGATGCT GCACGAGGCA
    GACGCGGGGC ACCTGAGCTT CGTGGAGGAG GTGTTTGAGA ACCAGGTGCG GCTGCCGGGC
    GGGCAGTGGA TCCACATGTC CGATGCCTAC ACCGATGTGA ATGGGGAGAA GGTTCTGCCC
    AAGGATGAGA TCGAGTGTCC TCCGGGCTGG AAATGGGAAG ACGTGGAGTG GGACACTGAC
    CTCAACAGAG CCGTGGATGA GAAAGGCTGG GAGTACGGCA TCACCATCCC TCCGGACCGC
    CGGCCCAAGG CCTGGGTGCC AGCTGAGAAG ATGTACCACA CCAACCGGCG CCGGCGCTGG
    GTGCGACTGC GCAGCAGGGA CCTGGCCCAG ATGGAGACTG TCAGGAAGCA CAAGCAGGAC
    GAGCAGGACG GCGAGGGCTG GGAATACGCC TCGCTGCTGG GCTGGCGCTT CCACCTGCGG
    CCGCGTCGCA CCGATGCCTT CCGCCGCCGC CGTTGGAGAC GTAGGATGGA GCCCTTGGAG
    CGCACCGGGG CTGCTGCTGT GTTCGCCTTG GAGGGGGCCC TGGGAGGAAT GACGGACGAC
    AAAAGCGACG ATGGCAAATC CGTCTCCACG CTGAGTTTCG GCGTCAACAG ACCCACCATC
    TCCTGCATCT TCGACTATGG GAACCGCTAC CACCTGCGCT GCTACATGTA CCAGGCACGG
    GACCTGGCTG CCATGGACAA GGACAGCTTC TCGGACCCCT ATGCCATCGT TTCCTTCCTC
    CACCAAAGCC AGAAGACGGT GGTGATCAAG AACACCCTGA ACCCCACGTG GGACCAGACG
    CTCATCTTCT ACGAGATAGA GATCTTCGGA GACCCCAAGA ACGTGTCTGA CTGCCCTCCC
    AGCATCGTGG TGGAGATATA CGACCACGAC ACCTACGGCG CTGATGAGTT CATGGGGCGC
    TGCGTGTGCC GGCCCAGCCT GCAGCGCGCG CCGCGCCTCT CCTGGCACCC CGTGCTCAAA
    GGCGGCAGGA ACGTCGGCGA GCTGCTGGCT GCCTTCGAGC TCATCCAGAG GGAGAAGCCG
    GCTGTCCACC ACATCCCTGG CTTCGAGAAC GAGCTGTCCT CCGCTCTGGA TGAGTCTGAG
    GACTCTGACC TGCCCTACCC ACCGCCCCAA CGGGAACCCA ACATCTTCAT GGTCCCCCAA
    GGGATCAAAC CGGTCCTGCA GCGCACGGCC ATCGAGATCC TGGCCTGGGG GCTGAGGAAC
    CTCAAGAGCT ACCAGCTGGC CAGCGTCACC TCACCCAGCC TGCTGGTGGA GTGCGGGGGG
    CAGCTCGTGC AGTCCTGTGT CATCAAGAAC GTCAAGAAAA ACCCCAACTT TGATGTCTGT
    GTGCTCTTCA TGGAAGTGCG TCTGCCCAGC GAGAGCCTCT ACAGTCCCCC CATCATCATC
    AAAATCATCG ACAACCGTCC GTTCGGCCGC AGGCCCGTGG TGGGGCAGTG CACCATCCGC
    TCACTGGAGG AATTCTACTG TGACCCCTAT GGTGAGGAGA CGGATGGTGC CCAGGAGCAG
    GCAGATGATG TGAGCCTCAC GCCCAGGGAT GATGTCCTCA TTGACATTGA TGACAAAGAG
    CCTCTCCTTC CCACCCAGCT TGTGGAGGGC GTGACCAGCT CAGCGCTAAT TAACCCGCCA
    GCTTCTAGGT CCGAGCCTCA AGAGGAAGAA TTCATTGACT GGTGGAGCAA ATTCTACGCT
    TCCACAGGAG AGAGGGAGAA GTGTGGCTCC TACTTGGAGA AGGGCTTTGA CACCCTGCAG
    GTCTATGAGA CGGAGCTGGA GCGCGTGGAG GCCTTCGAGC AGCTCTCTGA CTTCTGCCAC
    ACCTTCAGGC TGTACCGTGG CCGGGTGCAG GATGCCAACG ATGATCCCTC AGTTGTGGGC
    GAGTTCAAGG GCTCCTTCAA AATCTACCCT CTGCCCGATG ACCCACGCGT GCCGCTGCCG
    CCGCGGCAGT TCCACCAACT GCCAGCCAGG GGTCCCCAGG AGTGTCTCGT CAGGGTCTAC
    ATCATCCGTG CCTTCGGTCT GCAGCCCAAG GATGCCAATG GGAAGTGCGA TCCGTATGTG
    AAGATTTCAG TGGGAAAGAA ATCCATAAAC GACCAAGAAA ATTACCTCCC CTCTACGCTG
    GAGCCTGTCT TTGGAAAGAT GTTTGAGTTG AGCTGCACTC TGCCCCTGGA GAAGGACCTG
    AAGGTCACAC TCTATGACTA CGACCTCCTG TCCAAGGATG AGAAGATTGG GGAGACCGTC
    ATCGACCTGG AGAATCGGTT CCTGTCGAAA TATGGGGCGC GCTGTGGGCT TCCCCAGACC
    TACTGCATCT CTGGGCCCAA CCAGTGGCGA GACCAGCTCC GTCCCTCCCA ACTGCTCCAC
    CTCTTCTCCC TGCAGCACCA CTACAAGGCT CCCACCTACA CGTCCGACCG GCTCATCTTC
    CGGGAGCAGG AGTACATCCT CTCCGAGCTG GAGGATGGGA AACCCCCCAA CCCTCACCTG
    GGGCCGGCAG AGGAGCGGCT GGCCCTGCAT GCACTGCGGA AACAAGGGCT GGTGCCGGAG
    CACGTGGAGA CCCGGCCCCT CTACAGCCCC CTGCAGCCAG AGATCGAGCA GGGAAAGCTA
    CAGATGTGGG TGGACCTGTT TCCGAAATCC TTGGGTCAGC CTGGCCCCCC TTTCAACATC
    ACACCACGCA AAGCCAAGAG GTTCTACTTG CGCTGCATCA TCTGGAACAC CAGGGACGTC
    ATCCTCGATG ACCTCAGCAT CACTGGGGAG AAGATGAGTG ACATCTATGT CAAAGGCTGG
    CTGGTTGGCC ACGAGGAGAA CAAGCAGAAG ACAGACGTGC ACTACAGGTC GATGGGAGGA
    GAAGGCAACT TCAACTGGAG GTTCATCTTC CCCTTCGACT ATCTCCCTGC CGAGCAGATG
    TGTTGCGTTG CAAAGAAGGA GCATTTCTGG AGCTTGGATA AGACGGAAAA CAAGATTTCA
    CCGCAGCTGA TCTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTCGATGA CTATTTGGGC
    TCCATCCAGA TGGATCTCAA CAGGATGCCC AAGCCTGCCA AGACTGCTGA GAAGTGCTCC
    TTAGAGCTCA TAGATGACAG CTTGTCTGCA AGCCGCTTTG TGTCCCTCTT TGAGCAGAAG
    ACTGTCAAGG GCTGGTGGCC CTGCATTGCA GAGCAGGATG AGAAGAAGAT CCTGGCAGGA
    AAACTGGAAA TGACTTTGGA AATCGTGGCT GAACACGAGC ATGAAGAGCG GCCAGCTGGC
    GTGGGTCGGG ATGAGCCAAA CATGAACCCC AAGCTGGAGG ACCCCAAGCG CCCAGAGACC
    TCCTTCCTGT GGTTCACCTC CCCGTACAAG ACCCTGAAGT ACATCCTGTG GCGCCGGTAC
    AAGTGGCTGC TCATCCTGGC CATCGTGCTC TTTGTTTTGC TGCTCTTCCT TGGGATCTTC
    ATCTACGCCT TCCCGAACTA CGCTGCTATG AAGCTGGTGA AACCTTTCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150244
    Clone ID Related Accession (Same CDS sequence) XM_025149872.1
    Accession Version XM_025149872.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGAAGTGGCC
    ACCGAGGAGG AGGGGGAGGA CCAGGCGGTG ACGGGGGACG AGACGGAGCC CCCATCCTCG
    GGGCCGCCGG GCTCGGAGCC GCCCTCGGTG CCCCACAGAC CCCCGGCTCC CCGCGGCCGT
    GGGGTGAAGC GCAGGAGGAG CTCGCCCAAA AAGCCGCTGT CCAACAAACC TCAGGACTTC
    CAGATCCGGG TTCGGGTGAT CGAGGCCCGG CAGCTGCCCG GCGTTAACAT CCGGCCCGTG
    GTGAAGGTGA CGGCGGCGGG GCAGACCAAG CGGACCAGGA TACGCAAAGG CAACAGCCCC
    CTCTTCGACG AGACCTTCTT CTTCAACGTC TTCGAGTCGC CAGCCGAGCT GTTCGACGTG
    CCCGTCTTCA TCACGGTCCT GGACTCGCGC TCCTTCCGCA TGGATGCGGT GATCGGAGAG
    TTTCGGATGG ACGTTGAGAG CATCTATTCG GAGCCGAAAC ACGCGATCCT CAGGCGGTGG
    CTGCTGCTCT CTGACCCGGA GGACTTCACG GCGGGGGCAA AGGGGTACCT GAAGGTCAGC
    CTGCTTGTGC TGGGCCCCGG GGACGAAGCT CCTGTGGAGA AGAAGGACGT CTCTGAAGAC
    AAGGAAGACA TTGAGGGGAA CCTCCTGCGA CCCACGGGGG TCACCTTGCG AGGGGCTCAG
    TTCTGCCTGA AGATCTTCAA GGCTGAGGAT TTGCCCCAGA TGGACGACGC CGTCATGGAC
    AACGTCCGGC AGATCTTCGG CTTCGAAAGC AACAAGAAGA ACCTGGTTGA CCCCTTTGTG
    GAAGTCAGCT TTGCGGGGAA GACGCTCTAC TCGAGGATCC TGGAGAAGAA TGCCAACCCG
    CAGTGGAATC AGTGCCTGAT GCTGCCGGTG ATGTTCCCTT CCATGTGCGA GAAGATGAGG
    ATCCGGGTGA CCGACTGGGA CCGTCTCACA CACAACGACG TCGTCGGCAC CGCCTACCTC
    TGCATGTCCA AGATCTCTGC GCCCGGCGGG GAGCTGGAGG ATGCGTTTCC TGCTCTCACG
    AAGCCTCTGA AGGCCACAGA CCTGGACGAC GGGCTGGGCT TCCTGCCGAC CTTCGGCCCC
    TGCTACGTCA ACCTGTACGG CAGCCCCCGT GAGTTCACCG GCTTCCCGGA TCCGTACGAG
    GCGCTCAATT TAGGAAAGGG CGAGGGCGTC GCGTACCGCG GCCGGGTGCT GCTGGAGCTG
    GAGACCAAGC TGGCGGAGCA CGGGGAGCTG AAGGTGGAGG ACATCTGTGC GGATGACATC
    CTGCGGGTGG AGAAGTACCT GCGGCGGAGG AAGTACTCCC TCTTTGCTGC CTTCTACTCG
    GCCACCATGC TGCAGGACGT GGACGATGCC ATCCAGTTTG AGGTCAGCAT CGGCAACTAC
    GGCAACAAGT TCGACAACAC CTGCTTGCCC TTGGCCTCCA CCACGCAGTA CAGCCGGGCC
    GTGTTCGATG GTTGCCAGTA TTACTACCTG CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG
    CTGTCCTCCT ATTGGGAAGA TATCAGCTAC CGAACCGACA CTCAGAACCT CCTCCAGCAC
    GCAGCAGACA GGCTGGAAGC CAACCTGGAG CGGGTCCACC TCGCGCTCAA GGCCAACGGC
    GCGCCGGGCG AGCTGGAGTC CCTGGCTGCC CAGCTGATGG ACGACGTGAT TGCAGACTGC
    AGTGTTCCGC TGCCCGACGT GCTGGGGAAG GCGGCCAGCA CACACCTGGA CCAGAACCTG
    TACCGCTTCC GCAGCACCAA CCTGGAGCAG ATGGTGCGGG CGGCCCTGAG GCTGAAGCAC
    GAGGAGCTGA GCCTGCCGGC CGTGCTGGAG CAGGCGGAGG ACTGGCTGTG CCGGCTGCGT
    GCCATGGCTG AGGAGCCCCA GAACAGCCTC CCAGACGTGG TGATCTGGAT GCTGCGGGGT
    GAGAAGCGCG TTGCCTACGC CCGCGTGCCG GCCCGCGAGG TGCTCTACTC CCAAAGTGGC
    CCCAACTGCT GCGGTAGGAA CTGTGGCAAG CTGCAGACCA TCTTCCTGAA GTACCCCCAG
    GAGGAGGCGA TGGGGCCGCG CATCCCAGCC CAGATCCGCG TCCAGCTCTG GTTTGGGCTC
    TCTGTCAATG AGAAGGAGTT CAACCAATAC GCTGAGGGGA AGCTCTCCGT CTTTGCTGAA
    ACCTACGAGA ACCAGACCAA GCTGGCGCTG GTGGGGAACT GGGGCACCAC CGGCCTGACT
    TACCCCAAAT ACTCCGATGT CACCGGCAAG ATCAAGCTCC CCAAGGACAG CTTCCGGCCC
    TCCACGGGCT GGACCTGGGC TGGTGATTGG TTCATCTGCC CAGAGAAGAC GATGCTGCAC
    GAGGCAGACG CGGGGCACCT GAGCTTCGTG GAGGAGGTGT TTGAGAACCA GGTGCGGCTG
    CCGGGCGGGC AGTGGATCCA CATGTCCGAT GCCTACACCG ATGTGAATGG GGAGAAGGTT
    CTGCCCAAGG ATGAGATCGA GTGTCCTCCG GGCTGGAAAT GGGAAGACGT GGAGTGGGAC
    ACTGACCTCA ACAGAGCCGT GGATGAGAAA GGCTGGGAGT ACGGCATCAC CATCCCTCCG
    GACCGCCGGC CCAAGGCCTG GGTGCCAGCT GAGAAGATGT ACCACACCAA CCGGCGCCGG
    CGCTGGGTGC GACTGCGCAG CAGGGACCTG GCCCAGATGG AGACTGTCAG GAAGCACAAG
    CAGGACGAGC AGGACGGCGA GGGCTGGGAA TACGCCTCGC TGCTGGGCTG GCGCTTCCAC
    CTGCGGCCGC GTCGCACCGA TGCCTTCCGC CGCCGCCGTT GGAGACGTAG GATGGAGCCC
    TTGGAGCGCA CCGGGGCTGC TGCTGTGTTC GCCTTGGAGG GGGCCCTGGG AGGAATGACG
    GACGACAAAA GCGACGATGG CAAATCCGTC TCCACGCTGA GTTTCGGCGT CAACAGACCC
    ACCATCTCCT GCATCTTCGA CTATGGGAAC CGCTACCACC TGCGCTGCTA CATGTACCAG
    GCACGGGACC TGGCTGCCAT GGACAAGGAC AGCTTCTCGG ACCCCTATGC CATCGTTTCC
    TTCCTCCACC AAAGCCAGAA GACGGTGGTG ATCAAGAACA CCCTGAACCC CACGTGGGAC
    CAGACGCTCA TCTTCTACGA GATAGAGATC TTCGGAGACC CCAAGAACGT GTCTGACTGC
    CCTCCCAGCA TCGTGGTGGA GATATACGAC CACGACACCT ACGGCGCTGA TGAGTTCATG
    GGGCGCTGCG TGTGCCGGCC CAGCCTGCAG CGCGCGCCGC GCCTCTCCTG GCACCCCGTG
    CTCAAAGGCG GCAGGAACGT CGGCGAGCTG CTGGCTGCCT TCGAGCTCAT CCAGAGGGAG
    AAGCCGGCTG TCCACCACAT CCCTGGCTTC GAGAACGAGC TGTCCTCCGC TCTGGATGAG
    CTCTGTGCCC CTGCCTTGTT CTACGAGGGG CTCCTCCGAT GGTCTGAGGA CTCTGACCTG
    CCCTACCCAC CGCCCCAACG GGAACCCAAC ATCTTCATGG TCCCCCAAGG GATCAAACCG
    GTCCTGCAGC GCACGGCCAT CGAGATCCTG GCCTGGGGGC TGAGGAACCT CAAGAGCTAC
    CAGCTGGCCA GCGTCACCTC ACCCAGCCTG CTGGTGGAGT GCGGGGGGCA GCTCGTGCAG
    TCCTGTGTCA TCAAGAACGT CAAGAAAAAC CCCAACTTTG ATGTCTGTGT GCTCTTCATG
    GAAGTGCGTC TGCCCAGCGA GAGCCTCTAC AGTCCCCCCA TCATCATCAA AATCATCGAC
    AACCGTCCGT TCGGCCGCAG GCCCGTGGTG GGGCAGTGCA CCATCCGCTC ACTGGAGGAA
    TTCTACTGTG ACCCCTATGG TGAGGAGACG GATGGTGCCC AGGAGCAGGC AGATGATGTG
    AGCCTCACGC CCAGGGATGA TGTCCTCATT GACATTGATG ACAAAGAGCC TCTCCTTCCC
    ACCCAGCTTG TGGAGGGCGT GACCAGCTCA GCGCTAATTA ACCCGCCAGC TTCTAGGTCC
    GAGCCTCAAG AGGAAGAATT CATTGACTGG TGGAGCAAAT TCTACGCTTC CACAGGAGAG
    AGGGAGAAGT GTGGCTCCTA CTTGGAGAAG GGCTTTGACA CCCTGCAGGT CTATGAGACG
    GAGCTGGAGC GCGTGGAGGC CTTCGAGCAG CTCTCTGACT TCTGCCACAC CTTCAGGCTG
    TACCGTGGCC GGGTGCAGGA TGCCAACGAT GATCCCTCAG TTGTGGGCGA GTTCAAGGGC
    TCCTTCAAAA TCTACCCTCT GCCCGATGAC CCACGCGTGC CGCTGCCGCC GCGGCAGTTC
    CACCAACTGC CAGCCAGGGG TCCCCAGGAG TGTCTCGTCA GGGTCTACAT CATCCGTGCC
    TTCGGTCTGC AGCCCAAGGA TGCCAATGGG AAGTGCGATC CGTATGTGAA GATTTCAGTG
    GGAAAGAAAT CCATAAACGA CCAAGAAAAT TACCTCCCCT CTACGCTGGA GCCTGTCTTT
    GGAAAGATGT TTGAGTTGAG CTGCACTCTG CCCCTGGAGA AGGACCTGAA GGTCACACTC
    TATGACTACG ACCTCCTGTC CAAGGATGAG AAGATTGGGG AGACCGTCAT CGACCTGGAG
    AATCGGTTCC TGTCGAAATA TGGGGCGCGC TGTGGGCTTC CCCAGACCTA CTGCATCTCT
    GGGCCCAACC AGTGGCGAGA CCAGCTCCGT CCCTCCCAAC TGCTCCACCT CTTCTCCCTG
    CAGCACCACT ACAAGGCTCC CACCTACACG TCCGACCGGC TCATCTTCCG GGAGCAGGAG
    TACATCCTCT CCGAGCTGGA GGATGGGAAA CCCCCCAACC CTCACCTGGG GCCGGCAGAG
    GAGCGGCTGG CCCTGCATGC ACTGCGGAAA CAAGGGCTGG TGCCGGAGCA CGTGGAGACC
    CGGCCCCTCT ACAGCCCCCT GCAGCCAGAG ATCGAGCAGG GAAAGCTACA GATGTGGGTG
    GACCTGTTTC CGAAATCCTT GGGTCAGCCT GGCCCCCCTT TCAACATCAC ACCACGCAAA
    GCCAAGAGGT TCTACTTGCG CTGCATCATC TGGAACACCA GGGACGTCAT CCTCGATGAC
    CTCAGCATCA CTGGGGAGAA GATGAGTGAC ATCTATGTCA AAGGCTGGCT GGTTGGCCAC
    GAGGAGAACA AGCAGAAGAC AGACGTGCAC TACAGGTCGA TGGGAGGAGA AGGCAACTTC
    AACTGGAGGT TCATCTTCCC CTTCGACTAT CTCCCTGCCG AGCAGATGTG TTGCGTTGCA
    AAGAAGGAGC ATTTCTGGAG CTTGGATAAG ACGGAAAACA AGATTTCACC GCAGCTGATC
    TTCCAGATCT GGGACAATGA CAAGTTCTCC TTCGATGACT ATTTGGGCTC CATCCAGATG
    GATCTCAACA GGATGCCCAA GCCTGCCAAG ACTGCTGAGA AGTGCTCCTT AGAGCTCATA
    GATGACAGCT TGTCTGCAAG CCGCTTTGTG TCCCTCTTTG AGCAGAAGAC TGTCAAGGGC
    TGGTGGCCCT GCATTGCAGA GCAGGATGAG AAGAAGATCC TGGCAGGAAA ACTGGAAATG
    ACTTTGGAAA TCGTGGCTGA ACACGAGCAT GAAGAGCGGC CAGCTGGCGT GGGTCGGGAT
    GAGCCAAACA TGAACCCCAA GCTGGAGGAC CCCAAGCGCC CAGAGACCTC CTTCCTGTGG
    TTCACCTCCC CGTACAAGAC CCTGAAGTAC ATCCTGTGGC GCCGGTACAA GTGGCTGCTC
    ATCCTGGCCA TCGTGCTCTT TGTTTTGCTG CTCTTCCTTG GGATCTTCAT CTACGCCTTC
    CCGAACTACG CTGCTATGAA GCTGGTGAAA CCTTTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005640.1
    CDS647..7048
    Translation

    Target ORF information:

    RefSeq Version XM_025149872.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGAAGTGGCC
    ACCGAGGAGG AGGGGGAGGA CCAGGCGGTG ACGGGGGACG AGACGGAGCC CCCATCCTCG
    GGGCCGCCGG GCTCGGAGCC GCCCTCGGTG CCCCACAGAC CCCCGGCTCC CCGCGGCCGT
    GGGGTGAAGC GCAGGAGGAG CTCGCCCAAA AAGCCGCTGT CCAACAAACC TCAGGACTTC
    CAGATCCGGG TTCGGGTGAT CGAGGCCCGG CAGCTGCCCG GCGTTAACAT CCGGCCCGTG
    GTGAAGGTGA CGGCGGCGGG GCAGACCAAG CGGACCAGGA TACGCAAAGG CAACAGCCCC
    CTCTTCGACG AGACCTTCTT CTTCAACGTC TTCGAGTCGC CAGCCGAGCT GTTCGACGTG
    CCCGTCTTCA TCACGGTCCT GGACTCGCGC TCCTTCCGCA TGGATGCGGT GATCGGAGAG
    TTTCGGATGG ACGTTGAGAG CATCTATTCG GAGCCGAAAC ACGCGATCCT CAGGCGGTGG
    CTGCTGCTCT CTGACCCGGA GGACTTCACG GCGGGGGCAA AGGGGTACCT GAAGGTCAGC
    CTGCTTGTGC TGGGCCCCGG GGACGAAGCT CCTGTGGAGA AGAAGGACGT CTCTGAAGAC
    AAGGAAGACA TTGAGGGGAA CCTCCTGCGA CCCACGGGGG TCACCTTGCG AGGGGCTCAG
    TTCTGCCTGA AGATCTTCAA GGCTGAGGAT TTGCCCCAGA TGGACGACGC CGTCATGGAC
    AACGTCCGGC AGATCTTCGG CTTCGAAAGC AACAAGAAGA ACCTGGTTGA CCCCTTTGTG
    GAAGTCAGCT TTGCGGGGAA GACGCTCTAC TCGAGGATCC TGGAGAAGAA TGCCAACCCG
    CAGTGGAATC AGTGCCTGAT GCTGCCGGTG ATGTTCCCTT CCATGTGCGA GAAGATGAGG
    ATCCGGGTGA CCGACTGGGA CCGTCTCACA CACAACGACG TCGTCGGCAC CGCCTACCTC
    TGCATGTCCA AGATCTCTGC GCCCGGCGGG GAGCTGGAGG ATGCGTTTCC TGCTCTCACG
    AAGCCTCTGA AGGCCACAGA CCTGGACGAC GGGCTGGGCT TCCTGCCGAC CTTCGGCCCC
    TGCTACGTCA ACCTGTACGG CAGCCCCCGT GAGTTCACCG GCTTCCCGGA TCCGTACGAG
    GCGCTCAATT TAGGAAAGGG CGAGGGCGTC GCGTACCGCG GCCGGGTGCT GCTGGAGCTG
    GAGACCAAGC TGGCGGAGCA CGGGGAGCTG AAGGTGGAGG ACATCTGTGC GGATGACATC
    CTGCGGGTGG AGAAGTACCT GCGGCGGAGG AAGTACTCCC TCTTTGCTGC CTTCTACTCG
    GCCACCATGC TGCAGGACGT GGACGATGCC ATCCAGTTTG AGGTCAGCAT CGGCAACTAC
    GGCAACAAGT TCGACAACAC CTGCTTGCCC TTGGCCTCCA CCACGCAGTA CAGCCGGGCC
    GTGTTCGATG GTTGCCAGTA TTACTACCTG CCCTGGGGCA ACGTGAAGCC CGTGGTGGTG
    CTGTCCTCCT ATTGGGAAGA TATCAGCTAC CGAACCGACA CTCAGAACCT CCTCCAGCAC
    GCAGCAGACA GGCTGGAAGC CAACCTGGAG CGGGTCCACC TCGCGCTCAA GGCCAACGGC
    GCGCCGGGCG AGCTGGAGTC CCTGGCTGCC CAGCTGATGG ACGACGTGAT TGCAGACTGC
    AGTGTTCCGC TGCCCGACGT GCTGGGGAAG GCGGCCAGCA CACACCTGGA CCAGAACCTG
    TACCGCTTCC GCAGCACCAA CCTGGAGCAG ATGGTGCGGG CGGCCCTGAG GCTGAAGCAC
    GAGGAGCTGA GCCTGCCGGC CGTGCTGGAG CAGGCGGAGG ACTGGCTGTG CCGGCTGCGT
    GCCATGGCTG AGGAGCCCCA GAACAGCCTC CCAGACGTGG TGATCTGGAT GCTGCGGGGT
    GAGAAGCGCG TTGCCTACGC CCGCGTGCCG GCCCGCGAGG TGCTCTACTC CCAAAGTGGC
    CCCAACTGCT GCGGTAGGAA CTGTGGCAAG CTGCAGACCA TCTTCCTGAA GTACCCCCAG
    GAGGAGGCGA TGGGGCCGCG CATCCCAGCC CAGATCCGCG TCCAGCTCTG GTTTGGGCTC
    TCTGTCAATG AGAAGGAGTT CAACCAATAC GCTGAGGGGA AGCTCTCCGT CTTTGCTGAA
    ACCTACGAGA ACCAGACCAA GCTGGCGCTG GTGGGGAACT GGGGCACCAC CGGCCTGACT
    TACCCCAAAT ACTCCGATGT CACCGGCAAG ATCAAGCTCC CCAAGGACAG CTTCCGGCCC
    TCCACGGGCT GGACCTGGGC TGGTGATTGG TTCATCTGCC CAGAGAAGAC GATGCTGCAC
    GAGGCAGACG CGGGGCACCT GAGCTTCGTG GAGGAGGTGT TTGAGAACCA GGTGCGGCTG
    CCGGGCGGGC AGTGGATCCA CATGTCCGAT GCCTACACCG ATGTGAATGG GGAGAAGGTT
    CTGCCCAAGG ATGAGATCGA GTGTCCTCCG GGCTGGAAAT GGGAAGACGT GGAGTGGGAC
    ACTGACCTCA ACAGAGCCGT GGATGAGAAA GGCTGGGAGT ACGGCATCAC CATCCCTCCG
    GACCGCCGGC CCAAGGCCTG GGTGCCAGCT GAGAAGATGT ACCACACCAA CCGGCGCCGG
    CGCTGGGTGC GACTGCGCAG CAGGGACCTG GCCCAGATGG AGACTGTCAG GAAGCACAAG
    CAGGACGAGC AGGACGGCGA GGGCTGGGAA TACGCCTCGC TGCTGGGCTG GCGCTTCCAC
    CTGCGGCCGC GTCGCACCGA TGCCTTCCGC CGCCGCCGTT GGAGACGTAG GATGGAGCCC
    TTGGAGCGCA CCGGGGCTGC TGCTGTGTTC GCCTTGGAGG GGGCCCTGGG AGGAATGACG
    GACGACAAAA GCGACGATGG CAAATCCGTC TCCACGCTGA GTTTCGGCGT CAACAGACCC
    ACCATCTCCT GCATCTTCGA CTATGGGAAC CGCTACCACC TGCGCTGCTA CATGTACCAG
    GCACGGGACC TGGCTGCCAT GGACAAGGAC AGCTTCTCGG ACCCCTATGC CATCGTTTCC
    TTCCTCCACC AAAGCCAGAA GACGGTGGTG ATCAAGAACA CCCTGAACCC CACGTGGGAC
    CAGACGCTCA TCTTCTACGA GATAGAGATC TTCGGAGACC CCAAGAACGT GTCTGACTGC
    CCTCCCAGCA TCGTGGTGGA GATATACGAC CACGACACCT ACGGCGCTGA TGAGTTCATG
    GGGCGCTGCG TGTGCCGGCC CAGCCTGCAG CGCGCGCCGC GCCTCTCCTG GCACCCCGTG
    CTCAAAGGCG GCAGGAACGT CGGCGAGCTG CTGGCTGCCT TCGAGCTCAT CCAGAGGGAG
    AAGCCGGCTG TCCACCACAT CCCTGGCTTC GAGAACGAGC TGTCCTCCGC TCTGGATGAG
    CTCTGTGCCC CTGCCTTGTT CTACGAGGGG CTCCTCCGAT GGTCTGAGGA CTCTGACCTG
    CCCTACCCAC CGCCCCAACG GGAACCCAAC ATCTTCATGG TCCCCCAAGG GATCAAACCG
    GTCCTGCAGC GCACGGCCAT CGAGATCCTG GCCTGGGGGC TGAGGAACCT CAAGAGCTAC
    CAGCTGGCCA GCGTCACCTC ACCCAGCCTG CTGGTGGAGT GCGGGGGGCA GCTCGTGCAG
    TCCTGTGTCA TCAAGAACGT CAAGAAAAAC CCCAACTTTG ATGTCTGTGT GCTCTTCATG
    GAAGTGCGTC TGCCCAGCGA GAGCCTCTAC AGTCCCCCCA TCATCATCAA AATCATCGAC
    AACCGTCCGT TCGGCCGCAG GCCCGTGGTG GGGCAGTGCA CCATCCGCTC ACTGGAGGAA
    TTCTACTGTG ACCCCTATGG TGAGGAGACG GATGGTGCCC AGGAGCAGGC AGATGATGTG
    AGCCTCACGC CCAGGGATGA TGTCCTCATT GACATTGATG ACAAAGAGCC TCTCCTTCCC
    ACCCAGCTTG TGGAGGGCGT GACCAGCTCA GCGCTAATTA ACCCGCCAGC TTCTAGGTCC
    GAGCCTCAAG AGGAAGAATT CATTGACTGG TGGAGCAAAT TCTACGCTTC CACAGGAGAG
    AGGGAGAAGT GTGGCTCCTA CTTGGAGAAG GGCTTTGACA CCCTGCAGGT CTATGAGACG
    GAGCTGGAGC GCGTGGAGGC CTTCGAGCAG CTCTCTGACT TCTGCCACAC CTTCAGGCTG
    TACCGTGGCC GGGTGCAGGA TGCCAACGAT GATCCCTCAG TTGTGGGCGA GTTCAAGGGC
    TCCTTCAAAA TCTACCCTCT GCCCGATGAC CCACGCGTGC CGCTGCCGCC GCGGCAGTTC
    CACCAACTGC CAGCCAGGGG TCCCCAGGAG TGTCTCGTCA GGGTCTACAT CATCCGTGCC
    TTCGGTCTGC AGCCCAAGGA TGCCAATGGG AAGTGCGATC CGTATGTGAA GATTTCAGTG
    GGAAAGAAAT CCATAAACGA CCAAGAAAAT TACCTCCCCT CTACGCTGGA GCCTGTCTTT
    GGAAAGATGT TTGAGTTGAG CTGCACTCTG CCCCTGGAGA AGGACCTGAA GGTCACACTC
    TATGACTACG ACCTCCTGTC CAAGGATGAG AAGATTGGGG AGACCGTCAT CGACCTGGAG
    AATCGGTTCC TGTCGAAATA TGGGGCGCGC TGTGGGCTTC CCCAGACCTA CTGCATCTCT
    GGGCCCAACC AGTGGCGAGA CCAGCTCCGT CCCTCCCAAC TGCTCCACCT CTTCTCCCTG
    CAGCACCACT ACAAGGCTCC CACCTACACG TCCGACCGGC TCATCTTCCG GGAGCAGGAG
    TACATCCTCT CCGAGCTGGA GGATGGGAAA CCCCCCAACC CTCACCTGGG GCCGGCAGAG
    GAGCGGCTGG CCCTGCATGC ACTGCGGAAA CAAGGGCTGG TGCCGGAGCA CGTGGAGACC
    CGGCCCCTCT ACAGCCCCCT GCAGCCAGAG ATCGAGCAGG GAAAGCTACA GATGTGGGTG
    GACCTGTTTC CGAAATCCTT GGGTCAGCCT GGCCCCCCTT TCAACATCAC ACCACGCAAA
    GCCAAGAGGT TCTACTTGCG CTGCATCATC TGGAACACCA GGGACGTCAT CCTCGATGAC
    CTCAGCATCA CTGGGGAGAA GATGAGTGAC ATCTATGTCA AAGGCTGGCT GGTTGGCCAC
    GAGGAGAACA AGCAGAAGAC AGACGTGCAC TACAGGTCGA TGGGAGGAGA AGGCAACTTC
    AACTGGAGGT TCATCTTCCC CTTCGACTAT CTCCCTGCCG AGCAGATGTG TTGCGTTGCA
    AAGAAGGAGC ATTTCTGGAG CTTGGATAAG ACGGAAAACA AGATTTCACC GCAGCTGATC
    TTCCAGATCT GGGACAATGA CAAGTTCTCC TTCGATGACT ATTTGGGCTC CATCCAGATG
    GATCTCAACA GGATGCCCAA GCCTGCCAAG ACTGCTGAGA AGTGCTCCTT AGAGCTCATA
    GATGACAGCT TGTCTGCAAG CCGCTTTGTG TCCCTCTTTG AGCAGAAGAC TGTCAAGGGC
    TGGTGGCCCT GCATTGCAGA GCAGGATGAG AAGAAGATCC TGGCAGGAAA ACTGGAAATG
    ACTTTGGAAA TCGTGGCTGA ACACGAGCAT GAAGAGCGGC CAGCTGGCGT GGGTCGGGAT
    GAGCCAAACA TGAACCCCAA GCTGGAGGAC CCCAAGCGCC CAGAGACCTC CTTCCTGTGG
    TTCACCTCCC CGTACAAGAC CCTGAAGTAC ATCCTGTGGC GCCGGTACAA GTGGCTGCTC
    ATCCTGGCCA TCGTGCTCTT TGTTTTGCTG CTCTTCCTTG GGATCTTCAT CTACGCCTTC
    CCGAACTACG CTGCTATGAA GCTGGTGAAA CCTTTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150245
    Clone ID Related Accession (Same CDS sequence) XM_025149873.1
    Accession Version XM_025149873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGATGC GTTTCCTGCT CTCACGAAGC CTCTGAAGGC CACAGACCTG
    GACGACGGGC TGGGCTTCCT GCCGACCTTC GGCCCCTGCT ACGTCAACCT GTACGGCAGC
    CCCCGTGAGT TCACCGGCTT CCCGGATCCG TACGAGGCGC TCAATTTAGG AAAGGGCGAG
    GGCGTCGCGT ACCGCGGCCG GGTGCTGCTG GAGCTGGAGA CCAAGCTGGC GGAGCACGGG
    GAGCTGAAGG TGGAGGACAT CTGTGCGGAT GACATCCTGC GGGTGGAGAA GTACCTGCGG
    CGGAGGAAGT ACTCCCTCTT TGCTGCCTTC TACTCGGCCA CCATGCTGCA GGACGTGGAC
    GATGCCATCC AGTTTGAGGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CAACACCTGC
    TTGCCCTTGG CCTCCACCAC GCAGTACAGC CGGGCCGTGT TCGATGGTTG CCAGTATTAC
    TACCTGCCCT GGGGCAACGT GAAGCCCGTG GTGGTGCTGT CCTCCTATTG GGAAGATATC
    AGCTACCGAA CCGACACTCA GAACCTCCTC CAGCACGCAG CAGACAGGCT GGAAGCCAAC
    CTGGAGCGGG TCCACCTCGC GCTCAAGGCC AACGGCGCGC CGGGCGAGCT GGAGTCCCTG
    GCTGCCCAGC TGATGGACGA CGTGATTGCA GACTGCAGTG TTCCGCTGCC CGACGTGCTG
    GGGAAGGCGG CCAGCACACA CCTGGACCAG AACCTGTACC GCTTCCGCAG CACCAACCTG
    GAGCAGATGG TGCGGGCGGC CCTGAGGCTG AAGCACGAGG AGCTGAGCCT GCCGGCCGTG
    CTGGAGCAGG CGGAGGACTG GCTGTGCCGG CTGCGTGCCA TGGCTGAGGA GCCCCAGAAC
    AGCCTCCCAG ACGTGGTGAT CTGGATGCTG CGGGGTGAGA AGCGCGTTGC CTACGCCCGC
    GTGCCGGCCC GCGAGGTGCT CTACTCCCAA AGTGGCCCCA ACTGCTGCGG TAGGAACTGT
    GGCAAGCTGC AGACCATCTT CCTGAAGTAC CCCCAGGAGG AGGCGATGGG GCCGCGCATC
    CCAGCCCAGA TCCGCGTCCA GCTCTGGTTT GGGCTCTCTG TCAATGAGAA GGAGTTCAAC
    CAATACGCTG AGGGGAAGCT CTCCGTCTTT GCTGAAACCT ACGAGAACCA GACCAAGCTG
    GCGCTGGTGG GGAACTGGGG CACCACCGGC CTGACTTACC CCAAATACTC CGATGTCACC
    GGCAAGATCA AGCTCCCCAA GGACAGCTTC CGGCCCTCCA CGGGCTGGAC CTGGGCTGGT
    GATTGGTTCA TCTGCCCAGA GAAGACGATG CTGCACGAGG CAGACGCGGG GCACCTGAGC
    TTCGTGGAGG AGGTGTTTGA GAACCAGGTG CGGCTGCCGG GCGGGCAGTG GATCCACATG
    TCCGATGCCT ACACCGATGT GAATGGGGAG AAGGTTCTGC CCAAGGATGA GATCGAGTGT
    CCTCCGGGCT GGAAATGGGA AGACGTGGAG TGGGACACTG ACCTCAACAG AGCCGTGGAT
    GAGAAAGGCT GGGAGTACGG CATCACCATC CCTCCGGACC GCCGGCCCAA GGCCTGGGTG
    CCAGCTGAGA AGATGTACCA CACCAACCGG CGCCGGCGCT GGGTGCGACT GCGCAGCAGG
    GACCTGGCCC AGATGGAGAC TGTCAGGAAG CACAAGCAGG ACGAGCAGGA CGGCGAGGGC
    TGGGAATACG CCTCGCTGCT GGGCTGGCGC TTCCACCTGC GGCCGCGTCG CACCGATGCC
    TTCCGCCGCC GCCGTTGGAG ACGTAGGATG GAGCCCTTGG AGCGCACCGG GGCTGCTGCT
    GTGTTCGCCT TGGAGGGGGC CCTGGGAGGA ATGACGGACG ACAAAAGCGA CGATGGCAAA
    TCCGTCTCCA CGCTGAGTTT CGGCGTCAAC AGACCCACCA TCTCCTGCAT CTTCGACTAT
    GGGAACCGCT ACCACCTGCG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCCATGGAC
    AAGGACAGCT TCTCGGACCC CTATGCCATC GTTTCCTTCC TCCACCAAAG CCAGAAGACG
    GTGGTGATCA AGAACACCCT GAACCCCACG TGGGACCAGA CGCTCATCTT CTACGAGATA
    GAGATCTTCG GAGACCCCAA GAACGTGTCT GACTGCCCTC CCAGCATCGT GGTGGAGATA
    TACGACCACG ACACCTACGG CGCTGATGAG TTCATGGGGC GCTGCGTGTG CCGGCCCAGC
    CTGCAGCGCG CGCCGCGCCT CTCCTGGCAC CCCGTGCTCA AAGGCGGCAG GAACGTCGGC
    GAGCTGCTGG CTGCCTTCGA GCTCATCCAG AGGGAGAAGC CGGCTGTCCA CCACATCCCT
    GGCTTCGAGA ACGAGCTGTC CTCCGCTCTG GATGAGTCTG AGGACTCTGA CCTGCCCTAC
    CCACCGCCCC AACGGGAACC CAACATCTTC ATGGTCCCCC AAGGGATCAA ACCGGTCCTG
    CAGCGCACGG CCATCGAGAT CCTGGCCTGG GGGCTGAGGA ACCTCAAGAG CTACCAGCTG
    GCCAGCGTCA CCTCACCCAG CCTGCTGGTG GAGTGCGGGG GGCAGCTCGT GCAGTCCTGT
    GTCATCAAGA ACGTCAAGAA AAACCCCAAC TTTGATGTCT GTGTGCTCTT CATGGAAGTG
    CGTCTGCCCA GCGAGAGCCT CTACAGTCCC CCCATCATCA TCAAAATCAT CGACAACCGT
    CCGTTCGGCC GCAGGCCCGT GGTGGGGCAG TGCACCATCC GCTCACTGGA GGAATTCTAC
    TGTGACCCCT ATGGTGAGGA GACGGATGGT GCCCAGGAGC AGGCAGATGA TGTGAGCCTC
    ACGCCCAGGG ATGATGTCCT CATTGACATT GATGACAAAG AGCCTCTCCT TCCCACCCAG
    GAGGAAGAAT TCATTGACTG GTGGAGCAAA TTCTACGCTT CCACAGGAGA GAGGGAGAAG
    TGTGGCTCCT ACTTGGAGAA GGGCTTTGAC ACCCTGCAGG TCTATGAGAC GGAGCTGGAG
    CGCGTGGAGG CCTTCGAGCA GCTCTCTGAC TTCTGCCACA CCTTCAGGCT GTACCGTGGC
    CGGGTGCAGG ATGCCAACGA TGATCCCTCA GTTGTGGGCG AGTTCAAGGG CTCCTTCAAA
    ATCTACCCTC TGCCCGATGA CCCACGCGTG CCGCTGCCGC CGCGGCAGTT CCACCAACTG
    CCAGCCAGGG GTCCCCAGGA GTGTCTCGTC AGGGTCTACA TCATCCGTGC CTTCGGTCTG
    CAGCCCAAGG ATGCCAATGG GAAGTGCGAT CCGTATGTGA AGATTTCAGT GGGAAAGAAA
    TCCATAAACG ACCAAGAAAA TTACCTCCCC TCTACGCTGG AGCCTGTCTT TGGAAAGATG
    TTTGAGTTGA GCTGCACTCT GCCCCTGGAG AAGGACCTGA AGGTCACACT CTATGACTAC
    GACCTCCTGT CCAAGGATGA GAAGATTGGG GAGACCGTCA TCGACCTGGA GAATCGGTTC
    CTGTCGAAAT ATGGGGCGCG CTGTGGGCTT CCCCAGACCT ACTGCATCTC TGGGCCCAAC
    CAGTGGCGAG ACCAGCTCCG TCCCTCCCAA CTGCTCCACC TCTTCTCCCT GCAGCACCAC
    TACAAGGCTC CCACCTACAC GTCCGACCGG CTCATCTTCC GGGAGCAGGA GTACATCCTC
    TCCGAGCTGG AGGATGGGAA ACCCCCCAAC CCTCACCTGG GGCCGGCAGA GGAGCGGCTG
    GCCCTGCATG CACTGCGGAA ACAAGGGCTG GTGCCGGAGC ACGTGGAGAC CCGGCCCCTC
    TACAGCCCCC TGCAGCCAGA GATCGAGCAG GGAAAGCTAC AGATGTGGGT GGACCTGTTT
    CCGAAATCCT TGGGTCAGCC TGGCCCCCCT TTCAACATCA CACCACGCAA AGCCAAGAGG
    TTCTACTTGC GCTGCATCAT CTGGAACACC AGGGACGTCA TCCTCGATGA CCTCAGCATC
    ACTGGGGAGA AGATGAGTGA CATCTATGTC AAAGGCTGGC TGGTTGGCCA CGAGGAGAAC
    AAGCAGAAGA CAGACGTGCA CTACAGGTCG ATGGGAGGAG AAGGCAACTT CAACTGGAGG
    TTCATCTTCC CCTTCGACTA TCTCCCTGCC GAGCAGATGT GTTGCGTTGC AAAGAAGGAG
    CATTTCTGGA GCTTGGATAA GACGGAAAAC AAGATTTCAC CGCAGCTGAT CTTCCAGATC
    TGGGACAATG ACAAGTTCTC CTTCGATGAC TATTTGGGCT CCATCCAGAT GGATCTCAAC
    AGGATGCCCA AGCCTGCCAA GACTGCTGAG AAGTGCTCCT TAGAGCTCAT AGATGACAGC
    TTGTCTGCAA GCCGCTTTGT GTCCCTCTTT GAGCAGAAGA CTGTCAAGGG CTGGTGGCCC
    TGCATTGCAG AGCAGGATGA GAAGAAGATC CTGGCAGGAA AACTGGAAAT GACTTTGGAA
    ATCGTGGCTG AACACGAGCA TGAAGAGCGG CCAGCTGGCG TGGGTCGGGA TGAGCCAAAC
    ATGAACCCCA AGCTGGAGGA CCCCAAGCGC CCAGAGACCT CCTTCCTGTG GTTCACCTCC
    CCGTACAAGA CCCTGAAGTA CATCCTGTGG CGCCGGTACA AGTGGCTGCT CATCCTGGCC
    ATCGTGCTCT TTGTTTTGCT GCTCTTCCTT GGGATCTTCA TCTACGCCTT CCCGAACTAC
    GCTGCTATGA AGCTGGTGAA ACCTTTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005641.1
    CDS647..7039
    Translation

    Target ORF information:

    RefSeq Version XM_025149873.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGATGC GTTTCCTGCT CTCACGAAGC CTCTGAAGGC CACAGACCTG
    GACGACGGGC TGGGCTTCCT GCCGACCTTC GGCCCCTGCT ACGTCAACCT GTACGGCAGC
    CCCCGTGAGT TCACCGGCTT CCCGGATCCG TACGAGGCGC TCAATTTAGG AAAGGGCGAG
    GGCGTCGCGT ACCGCGGCCG GGTGCTGCTG GAGCTGGAGA CCAAGCTGGC GGAGCACGGG
    GAGCTGAAGG TGGAGGACAT CTGTGCGGAT GACATCCTGC GGGTGGAGAA GTACCTGCGG
    CGGAGGAAGT ACTCCCTCTT TGCTGCCTTC TACTCGGCCA CCATGCTGCA GGACGTGGAC
    GATGCCATCC AGTTTGAGGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CAACACCTGC
    TTGCCCTTGG CCTCCACCAC GCAGTACAGC CGGGCCGTGT TCGATGGTTG CCAGTATTAC
    TACCTGCCCT GGGGCAACGT GAAGCCCGTG GTGGTGCTGT CCTCCTATTG GGAAGATATC
    AGCTACCGAA CCGACACTCA GAACCTCCTC CAGCACGCAG CAGACAGGCT GGAAGCCAAC
    CTGGAGCGGG TCCACCTCGC GCTCAAGGCC AACGGCGCGC CGGGCGAGCT GGAGTCCCTG
    GCTGCCCAGC TGATGGACGA CGTGATTGCA GACTGCAGTG TTCCGCTGCC CGACGTGCTG
    GGGAAGGCGG CCAGCACACA CCTGGACCAG AACCTGTACC GCTTCCGCAG CACCAACCTG
    GAGCAGATGG TGCGGGCGGC CCTGAGGCTG AAGCACGAGG AGCTGAGCCT GCCGGCCGTG
    CTGGAGCAGG CGGAGGACTG GCTGTGCCGG CTGCGTGCCA TGGCTGAGGA GCCCCAGAAC
    AGCCTCCCAG ACGTGGTGAT CTGGATGCTG CGGGGTGAGA AGCGCGTTGC CTACGCCCGC
    GTGCCGGCCC GCGAGGTGCT CTACTCCCAA AGTGGCCCCA ACTGCTGCGG TAGGAACTGT
    GGCAAGCTGC AGACCATCTT CCTGAAGTAC CCCCAGGAGG AGGCGATGGG GCCGCGCATC
    CCAGCCCAGA TCCGCGTCCA GCTCTGGTTT GGGCTCTCTG TCAATGAGAA GGAGTTCAAC
    CAATACGCTG AGGGGAAGCT CTCCGTCTTT GCTGAAACCT ACGAGAACCA GACCAAGCTG
    GCGCTGGTGG GGAACTGGGG CACCACCGGC CTGACTTACC CCAAATACTC CGATGTCACC
    GGCAAGATCA AGCTCCCCAA GGACAGCTTC CGGCCCTCCA CGGGCTGGAC CTGGGCTGGT
    GATTGGTTCA TCTGCCCAGA GAAGACGATG CTGCACGAGG CAGACGCGGG GCACCTGAGC
    TTCGTGGAGG AGGTGTTTGA GAACCAGGTG CGGCTGCCGG GCGGGCAGTG GATCCACATG
    TCCGATGCCT ACACCGATGT GAATGGGGAG AAGGTTCTGC CCAAGGATGA GATCGAGTGT
    CCTCCGGGCT GGAAATGGGA AGACGTGGAG TGGGACACTG ACCTCAACAG AGCCGTGGAT
    GAGAAAGGCT GGGAGTACGG CATCACCATC CCTCCGGACC GCCGGCCCAA GGCCTGGGTG
    CCAGCTGAGA AGATGTACCA CACCAACCGG CGCCGGCGCT GGGTGCGACT GCGCAGCAGG
    GACCTGGCCC AGATGGAGAC TGTCAGGAAG CACAAGCAGG ACGAGCAGGA CGGCGAGGGC
    TGGGAATACG CCTCGCTGCT GGGCTGGCGC TTCCACCTGC GGCCGCGTCG CACCGATGCC
    TTCCGCCGCC GCCGTTGGAG ACGTAGGATG GAGCCCTTGG AGCGCACCGG GGCTGCTGCT
    GTGTTCGCCT TGGAGGGGGC CCTGGGAGGA ATGACGGACG ACAAAAGCGA CGATGGCAAA
    TCCGTCTCCA CGCTGAGTTT CGGCGTCAAC AGACCCACCA TCTCCTGCAT CTTCGACTAT
    GGGAACCGCT ACCACCTGCG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCCATGGAC
    AAGGACAGCT TCTCGGACCC CTATGCCATC GTTTCCTTCC TCCACCAAAG CCAGAAGACG
    GTGGTGATCA AGAACACCCT GAACCCCACG TGGGACCAGA CGCTCATCTT CTACGAGATA
    GAGATCTTCG GAGACCCCAA GAACGTGTCT GACTGCCCTC CCAGCATCGT GGTGGAGATA
    TACGACCACG ACACCTACGG CGCTGATGAG TTCATGGGGC GCTGCGTGTG CCGGCCCAGC
    CTGCAGCGCG CGCCGCGCCT CTCCTGGCAC CCCGTGCTCA AAGGCGGCAG GAACGTCGGC
    GAGCTGCTGG CTGCCTTCGA GCTCATCCAG AGGGAGAAGC CGGCTGTCCA CCACATCCCT
    GGCTTCGAGA ACGAGCTGTC CTCCGCTCTG GATGAGTCTG AGGACTCTGA CCTGCCCTAC
    CCACCGCCCC AACGGGAACC CAACATCTTC ATGGTCCCCC AAGGGATCAA ACCGGTCCTG
    CAGCGCACGG CCATCGAGAT CCTGGCCTGG GGGCTGAGGA ACCTCAAGAG CTACCAGCTG
    GCCAGCGTCA CCTCACCCAG CCTGCTGGTG GAGTGCGGGG GGCAGCTCGT GCAGTCCTGT
    GTCATCAAGA ACGTCAAGAA AAACCCCAAC TTTGATGTCT GTGTGCTCTT CATGGAAGTG
    CGTCTGCCCA GCGAGAGCCT CTACAGTCCC CCCATCATCA TCAAAATCAT CGACAACCGT
    CCGTTCGGCC GCAGGCCCGT GGTGGGGCAG TGCACCATCC GCTCACTGGA GGAATTCTAC
    TGTGACCCCT ATGGTGAGGA GACGGATGGT GCCCAGGAGC AGGCAGATGA TGTGAGCCTC
    ACGCCCAGGG ATGATGTCCT CATTGACATT GATGACAAAG AGCCTCTCCT TCCCACCCAG
    GAGGAAGAAT TCATTGACTG GTGGAGCAAA TTCTACGCTT CCACAGGAGA GAGGGAGAAG
    TGTGGCTCCT ACTTGGAGAA GGGCTTTGAC ACCCTGCAGG TCTATGAGAC GGAGCTGGAG
    CGCGTGGAGG CCTTCGAGCA GCTCTCTGAC TTCTGCCACA CCTTCAGGCT GTACCGTGGC
    CGGGTGCAGG ATGCCAACGA TGATCCCTCA GTTGTGGGCG AGTTCAAGGG CTCCTTCAAA
    ATCTACCCTC TGCCCGATGA CCCACGCGTG CCGCTGCCGC CGCGGCAGTT CCACCAACTG
    CCAGCCAGGG GTCCCCAGGA GTGTCTCGTC AGGGTCTACA TCATCCGTGC CTTCGGTCTG
    CAGCCCAAGG ATGCCAATGG GAAGTGCGAT CCGTATGTGA AGATTTCAGT GGGAAAGAAA
    TCCATAAACG ACCAAGAAAA TTACCTCCCC TCTACGCTGG AGCCTGTCTT TGGAAAGATG
    TTTGAGTTGA GCTGCACTCT GCCCCTGGAG AAGGACCTGA AGGTCACACT CTATGACTAC
    GACCTCCTGT CCAAGGATGA GAAGATTGGG GAGACCGTCA TCGACCTGGA GAATCGGTTC
    CTGTCGAAAT ATGGGGCGCG CTGTGGGCTT CCCCAGACCT ACTGCATCTC TGGGCCCAAC
    CAGTGGCGAG ACCAGCTCCG TCCCTCCCAA CTGCTCCACC TCTTCTCCCT GCAGCACCAC
    TACAAGGCTC CCACCTACAC GTCCGACCGG CTCATCTTCC GGGAGCAGGA GTACATCCTC
    TCCGAGCTGG AGGATGGGAA ACCCCCCAAC CCTCACCTGG GGCCGGCAGA GGAGCGGCTG
    GCCCTGCATG CACTGCGGAA ACAAGGGCTG GTGCCGGAGC ACGTGGAGAC CCGGCCCCTC
    TACAGCCCCC TGCAGCCAGA GATCGAGCAG GGAAAGCTAC AGATGTGGGT GGACCTGTTT
    CCGAAATCCT TGGGTCAGCC TGGCCCCCCT TTCAACATCA CACCACGCAA AGCCAAGAGG
    TTCTACTTGC GCTGCATCAT CTGGAACACC AGGGACGTCA TCCTCGATGA CCTCAGCATC
    ACTGGGGAGA AGATGAGTGA CATCTATGTC AAAGGCTGGC TGGTTGGCCA CGAGGAGAAC
    AAGCAGAAGA CAGACGTGCA CTACAGGTCG ATGGGAGGAG AAGGCAACTT CAACTGGAGG
    TTCATCTTCC CCTTCGACTA TCTCCCTGCC GAGCAGATGT GTTGCGTTGC AAAGAAGGAG
    CATTTCTGGA GCTTGGATAA GACGGAAAAC AAGATTTCAC CGCAGCTGAT CTTCCAGATC
    TGGGACAATG ACAAGTTCTC CTTCGATGAC TATTTGGGCT CCATCCAGAT GGATCTCAAC
    AGGATGCCCA AGCCTGCCAA GACTGCTGAG AAGTGCTCCT TAGAGCTCAT AGATGACAGC
    TTGTCTGCAA GCCGCTTTGT GTCCCTCTTT GAGCAGAAGA CTGTCAAGGG CTGGTGGCCC
    TGCATTGCAG AGCAGGATGA GAAGAAGATC CTGGCAGGAA AACTGGAAAT GACTTTGGAA
    ATCGTGGCTG AACACGAGCA TGAAGAGCGG CCAGCTGGCG TGGGTCGGGA TGAGCCAAAC
    ATGAACCCCA AGCTGGAGGA CCCCAAGCGC CCAGAGACCT CCTTCCTGTG GTTCACCTCC
    CCGTACAAGA CCCTGAAGTA CATCCTGTGG CGCCGGTACA AGTGGCTGCT CATCCTGGCC
    ATCGTGCTCT TTGTTTTGCT GCTCTTCCTT GGGATCTTCA TCTACGCCTT CCCGAACTAC
    GCTGCTATGA AGCTGGTGAA ACCTTTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150246
    Clone ID Related Accession (Same CDS sequence) XM_025149874.1
    Accession Version XM_025149874.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGTGGA CGACGGGCTG GGCTTCCTGC CGACCTTCGG CCCCTGCTAC
    GTCAACCTGT ACGGCAGCCC CCGTGAGTTC ACCGGCTTCC CGGATCCGTA CGAGGCGCTC
    AATTTAGGAA AGGGCGAGGG CGTCGCGTAC CGCGGCCGGG TGCTGCTGGA GCTGGAGACC
    AAGCTGGCGG AGCACGGGGA GCTGAAGGTG GAGGACATCT GTGCGGATGA CATCCTGCGG
    GTGGAGAAGT ACCTGCGGCG GAGGAAGTAC TCCCTCTTTG CTGCCTTCTA CTCGGCCACC
    ATGCTGCAGG ACGTGGACGA TGCCATCCAG TTTGAGGTCA GCATCGGCAA CTACGGCAAC
    AAGTTCGACA ACACCTGCTT GCCCTTGGCC TCCACCACGC AGTACAGCCG GGCCGTGTTC
    GATGGTTGCC AGTATTACTA CCTGCCCTGG GGCAACGTGA AGCCCGTGGT GGTGCTGTCC
    TCCTATTGGG AAGATATCAG CTACCGAACC GACACTCAGA ACCTCCTCCA GCACGCAGCA
    GACAGGCTGG AAGCCAACCT GGAGCGGGTC CACCTCGCGC TCAAGGCCAA CGGCGCGCCG
    GGCGAGCTGG AGTCCCTGGC TGCCCAGCTG ATGGACGACG TGATTGCAGA CTGCAGTGTT
    CCGCTGCCCG ACGTGCTGGG GAAGGCGGCC AGCACACACC TGGACCAGAA CCTGTACCGC
    TTCCGCAGCA CCAACCTGGA GCAGATGGTG CGGGCGGCCC TGAGGCTGAA GCACGAGGAG
    CTGAGCCTGC CGGCCGTGCT GGAGCAGGCG GAGGACTGGC TGTGCCGGCT GCGTGCCATG
    GCTGAGGAGC CCCAGAACAG CCTCCCAGAC GTGGTGATCT GGATGCTGCG GGGTGAGAAG
    CGCGTTGCCT ACGCCCGCGT GCCGGCCCGC GAGGTGCTCT ACTCCCAAAG TGGCCCCAAC
    TGCTGCGGTA GGAACTGTGG CAAGCTGCAG ACCATCTTCC TGAAGTACCC CCAGGAGGAG
    GCGATGGGGC CGCGCATCCC AGCCCAGATC CGCGTCCAGC TCTGGTTTGG GCTCTCTGTC
    AATGAGAAGG AGTTCAACCA ATACGCTGAG GGGAAGCTCT CCGTCTTTGC TGAAACCTAC
    GAGAACCAGA CCAAGCTGGC GCTGGTGGGG AACTGGGGCA CCACCGGCCT GACTTACCCC
    AAATACTCCG ATGTCACCGG CAAGATCAAG CTCCCCAAGG ACAGCTTCCG GCCCTCCACG
    GGCTGGACCT GGGCTGGTGA TTGGTTCATC TGCCCAGAGA AGACGATGCT GCACGAGGCA
    GACGCGGGGC ACCTGAGCTT CGTGGAGGAG GTGTTTGAGA ACCAGGTGCG GCTGCCGGGC
    GGGCAGTGGA TCCACATGTC CGATGCCTAC ACCGATGTGA ATGGGGAGAA GGTTCTGCCC
    AAGGATGAGA TCGAGTGTCC TCCGGGCTGG AAATGGGAAG ACGTGGAGTG GGACACTGAC
    CTCAACAGAG CCGTGGATGA GAAAGGCTGG GAGTACGGCA TCACCATCCC TCCGGACCGC
    CGGCCCAAGG CCTGGGTGCC AGCTGAGAAG ATGTACCACA CCAACCGGCG CCGGCGCTGG
    GTGCGACTGC GCAGCAGGGA CCTGGCCCAG ATGGAGACTG TCAGGAAGCA CAAGCAGGAC
    GAGCAGGACG GCGAGGGCTG GGAATACGCC TCGCTGCTGG GCTGGCGCTT CCACCTGCGG
    CCGCGTCGCA CCGATGCCTT CCGCCGCCGC CGTTGGAGAC GTAGGATGGA GCCCTTGGAG
    CGCACCGGGG CTGCTGCTGT GTTCGCCTTG GAGGGGGCCC TGGGAGGAAT GACGGACGAC
    AAAAGCGACG ATGGCAAATC CGTCTCCACG CTGAGTTTCG GCGTCAACAG ACCCACCATC
    TCCTGCATCT TCGACTATGG GAACCGCTAC CACCTGCGCT GCTACATGTA CCAGGCACGG
    GACCTGGCTG CCATGGACAA GGACAGCTTC TCGGACCCCT ATGCCATCGT TTCCTTCCTC
    CACCAAAGCC AGAAGACGGT GGTGATCAAG AACACCCTGA ACCCCACGTG GGACCAGACG
    CTCATCTTCT ACGAGATAGA GATCTTCGGA GACCCCAAGA ACGTGTCTGA CTGCCCTCCC
    AGCATCGTGG TGGAGATATA CGACCACGAC ACCTACGGCG CTGATGAGTT CATGGGGCGC
    TGCGTGTGCC GGCCCAGCCT GCAGCGCGCG CCGCGCCTCT CCTGGCACCC CGTGCTCAAA
    GGCGGCAGGA ACGTCGGCGA GCTGCTGGCT GCCTTCGAGC TCATCCAGAG GGAGAAGCCG
    GCTGTCCACC ACATCCCTGG CTTCGAGAAC GAGCTGTCCT CCGCTCTGGA TGAGTCTGAG
    GACTCTGACC TGCCCTACCC ACCGCCCCAA CGGGAACCCA ACATCTTCAT GGTCCCCCAA
    GGGATCAAAC CGGTCCTGCA GCGCACGGCC ATCGAGATCC TGGCCTGGGG GCTGAGGAAC
    CTCAAGAGCT ACCAGCTGGC CAGCGTCACC TCACCCAGCC TGCTGGTGGA GTGCGGGGGG
    CAGCTCGTGC AGTCCTGTGT CATCAAGAAC GTCAAGAAAA ACCCCAACTT TGATGTCTGT
    GTGCTCTTCA TGGAAGTGCG TCTGCCCAGC GAGAGCCTCT ACAGTCCCCC CATCATCATC
    AAAATCATCG ACAACCGTCC GTTCGGCCGC AGGCCCGTGG TGGGGCAGTG CACCATCCGC
    TCACTGGAGG AATTCTACTG TGACCCCTAT GGTGAGGAGA CGGATGGTGC CCAGGAGCAG
    GCAGATGATG TGAGCCTCAC GCCCAGGGAT GATGTCCTCA TTGACATTGA TGACAAAGAG
    CCTCTCCTTC CCACCCAGGA GGAAGAATTC ATTGACTGGT GGAGCAAATT CTACGCTTCC
    ACAGGAGAGA GGGAGAAGTG TGGCTCCTAC TTGGAGAAGG GCTTTGACAC CCTGCAGGTC
    TATGAGACGG AGCTGGAGCG CGTGGAGGCC TTCGAGCAGC TCTCTGACTT CTGCCACACC
    TTCAGGCTGT ACCGTGGCCG GGTGCAGGAT GCCAACGATG ATCCCTCAGT TGTGGGCGAG
    TTCAAGGGCT CCTTCAAAAT CTACCCTCTG CCCGATGACC CACGCGTGCC GCTGCCGCCG
    CGGCAGTTCC ACCAACTGCC AGCCAGGGGT CCCCAGGAGT GTCTCGTCAG GGTCTACATC
    ATCCGTGCCT TCGGTCTGCA GCCCAAGGAT GCCAATGGGA AGTGCGATCC GTATGTGAAG
    ATTTCAGTGG GAAAGAAATC CATAAACGAC CAAGAAAATT ACCTCCCCTC TACGCTGGAG
    CCTGTCTTTG GAAAGATGTT TGAGTTGAGC TGCACTCTGC CCCTGGAGAA GGACCTGAAG
    GTCACACTCT ATGACTACGA CCTCCTGTCC AAGGATGAGA AGATTGGGGA GACCGTCATC
    GACCTGGAGA ATCGGTTCCT GTCGAAATAT GGGGCGCGCT GTGGGCTTCC CCAGACCTAC
    TGCATCTCTG GGCCCAACCA GTGGCGAGAC CAGCTCCGTC CCTCCCAACT GCTCCACCTC
    TTCTCCCTGC AGCACCACTA CAAGGCTCCC ACCTACACGT CCGACCGGCT CATCTTCCGG
    GAGCAGGAGT ACATCCTCTC CGAGCTGGAG GATGGGAAAC CCCCCAACCC TCACCTGGGG
    CCGGCAGAGG AGCGGCTGGC CCTGCATGCA CTGCGGAAAC AAGGGCTGGT GCCGGAGCAC
    GTGGAGACCC GGCCCCTCTA CAGCCCCCTG CAGCCAGAGA TCGAGCAGGG AAAGCTACAG
    ATGTGGGTGG ACCTGTTTCC GAAATCCTTG GGTCAGCCTG GCCCCCCTTT CAACATCACA
    CCACGCAAAG CCAAGAGGTT CTACTTGCGC TGCATCATCT GGAACACCAG GGACGTCATC
    CTCGATGACC TCAGCATCAC TGGGGAGAAG ATGAGTGACA TCTATGTCAA AGGCTGGCTG
    GTTGGCCACG AGGAGAACAA GCAGAAGACA GACGTGCACT ACAGGTCGAT GGGAGGAGAA
    GGCAACTTCA ACTGGAGGTT CATCTTCCCC TTCGACTATC TCCCTGCCGA GCAGATGTGT
    TGCGTTGCAA AGAAGGAGCA TTTCTGGAGC TTGGATAAGA CGGAAAACAA GATTTCACCG
    CAGCTGATCT TCCAGATCTG GGACAATGAC AAGTTCTCCT TCGATGACTA TTTGGGCTCC
    ATCCAGATGG ATCTCAACAG GATGCCCAAG CCTGCCAAGA CTGCTGAGAA GTGCTCCTTA
    GAGCTCATAG ATGACAGCTT GTCTGCAAGC CGCTTTGTGT CCCTCTTTGA GCAGAAGACT
    GTCAAGGGCT GGTGGCCCTG CATTGCAGAG CAGGATGAGA AGAAGATCCT GGCAGGAAAA
    CTGGAAATGA CTTTGGAAAT CGTGGCTGAA CACGAGCATG AAGAGCGGCC AGCTGGCGTG
    GGTCGGGATG AGCCAAACAT GAACCCCAAG CTGGAGGACC CCAAGCGCCC AGAGACCTCC
    TTCCTGTGGT TCACCTCCCC GTACAAGACC CTGAAGTACA TCCTGTGGCG CCGGTACAAG
    TGGCTGCTCA TCCTGGCCAT CGTGCTCTTT GTTTTGCTGC TCTTCCTTGG GATCTTCATC
    TACGCCTTCC CGAACTACGC TGCTATGAAG CTGGTGAAAC CTTTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005642.1
    CDS647..6997
    Translation

    Target ORF information:

    RefSeq Version XM_025149874.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149874.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGGATCTA TGGGGGAAAC TGAGGCACAG CATATGGGAT CCATCGGGCA CAGTTTATGG 
    GATCTATGGG GGAAACTGAG GCACAGCCTC CGTGCTGGAA AACCCCATTG CTTTTCCCCC
    CCATGTGTGG GATGGGGGCT GAGTTTTGGG GCCGTTGTAG GGGTGAAGAA GAAGACGAAG
    GTCATCAAGA ACAGCATCAA CCCCGTATGG AACGAGGGCT TTGAATGGGA TCTGAAGGGC
    GTTGCTTTGG ACCTGGGATC TGAGCTCTGT GTTGTGGTTA AGGACCATGA GACTGTGGGG
    AGGAATCGGT TTTTGGGAGA GGCCCGCGTC CCCCTACGGG ATGTGCTGTC CTCCCCCAGC
    CTGGCGGCCT CCTATGATGT CCCATTGCTG GATAGCAAGA AGCAGAGCAC CGGGGCTTCC
    CTCATCCTAC AAGTGTCCTA CAGCCCTCCT CCGGGCGCTG CCCCCCTCTT CCCTCCCCCA
    ACCCCCCCCG AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGGTAATGCG
    GGGGCTCCAC GGGGGGACGC GTCCCCCATA GCCCACCCCA CAGCGGCCAG GGACCCGCAG
    CAACCTCCCT GCCTCCCGCC CCGCGCAGAA GTGGCCACCG AGGAGGAGGG GGAGGACCAG
    GCGGTGACGG GGGACGAGAC GGAGCCCCCA TCCTCGGGGC CGCCGGGCTC GGAGCCGCCC
    TCGGTGCCCC ACAGACCCCC GGCTCCCCGC GGCCGTGGGG TGAAGCGCAG GAGGAGCTCG
    CCCAAAAAGC CGCTGTCCAA CAAACCTCAG GACTTCCAGA TCCGGGTTCG GGTGATCGAG
    GCCCGGCAGC TGCCCGGCGT TAACATCCGG CCCGTGGTGA AGGTGACGGC GGCGGGGCAG
    ACCAAGCGGA CCAGGATACG CAAAGGCAAC AGCCCCCTCT TCGACGAGAC CTTCTTCTTC
    AACGTCTTCG AGTCGCCAGC CGAGCTGTTC GACGTGCCCG TCTTCATCAC GGTCCTGGAC
    TCGCGCTCCT TCCGCATGGA TGCGGTGATC GGAGAGTTTC GGATGGACGT TGAGAGCATC
    TATTCGGAGC CGAAACACGC GATCCTCAGG CGGTGGCTGC TGCTCTCTGA CCCGGAGGAC
    TTCACGGCGG GGGCAAAGGG GTACCTGAAG GTCAGCCTGC TTGTGCTGGG CCCCGGGGAC
    GAAGCTCCTG TGGAGAAGAA GGACGTCTCT GAAGACAAGG AAGACATTGA GGGGAACCTC
    CTGCGACCCA CGGGGGTCAC CTTGCGAGGG GCTCAGTTCT GCCTGAAGAT CTTCAAGGCT
    GAGGATTTGC CCCAGATGGA CGACGCCGTC ATGGACAACG TCCGGCAGAT CTTCGGCTTC
    GAAAGCAACA AGAAGAACCT GGTTGACCCC TTTGTGGAAG TCAGCTTTGC GGGGAAGACG
    CTCTACTCGA GGATCCTGGA GAAGAATGCC AACCCGCAGT GGAATCAGTG CCTGATGCTG
    CCGGTGATGT TCCCTTCCAT GTGCGAGAAG ATGAGGATCC GGGTGACCGA CTGGGACCGT
    CTCACACACA ACGACGTCGT CGGCACCGCC TACCTCTGCA TGTCCAAGAT CTCTGCGCCC
    GGCGGGGAGC TGGAGGTGGA CGACGGGCTG GGCTTCCTGC CGACCTTCGG CCCCTGCTAC
    GTCAACCTGT ACGGCAGCCC CCGTGAGTTC ACCGGCTTCC CGGATCCGTA CGAGGCGCTC
    AATTTAGGAA AGGGCGAGGG CGTCGCGTAC CGCGGCCGGG TGCTGCTGGA GCTGGAGACC
    AAGCTGGCGG AGCACGGGGA GCTGAAGGTG GAGGACATCT GTGCGGATGA CATCCTGCGG
    GTGGAGAAGT ACCTGCGGCG GAGGAAGTAC TCCCTCTTTG CTGCCTTCTA CTCGGCCACC
    ATGCTGCAGG ACGTGGACGA TGCCATCCAG TTTGAGGTCA GCATCGGCAA CTACGGCAAC
    AAGTTCGACA ACACCTGCTT GCCCTTGGCC TCCACCACGC AGTACAGCCG GGCCGTGTTC
    GATGGTTGCC AGTATTACTA CCTGCCCTGG GGCAACGTGA AGCCCGTGGT GGTGCTGTCC
    TCCTATTGGG AAGATATCAG CTACCGAACC GACACTCAGA ACCTCCTCCA GCACGCAGCA
    GACAGGCTGG AAGCCAACCT GGAGCGGGTC CACCTCGCGC TCAAGGCCAA CGGCGCGCCG
    GGCGAGCTGG AGTCCCTGGC TGCCCAGCTG ATGGACGACG TGATTGCAGA CTGCAGTGTT
    CCGCTGCCCG ACGTGCTGGG GAAGGCGGCC AGCACACACC TGGACCAGAA CCTGTACCGC
    TTCCGCAGCA CCAACCTGGA GCAGATGGTG CGGGCGGCCC TGAGGCTGAA GCACGAGGAG
    CTGAGCCTGC CGGCCGTGCT GGAGCAGGCG GAGGACTGGC TGTGCCGGCT GCGTGCCATG
    GCTGAGGAGC CCCAGAACAG CCTCCCAGAC GTGGTGATCT GGATGCTGCG GGGTGAGAAG
    CGCGTTGCCT ACGCCCGCGT GCCGGCCCGC GAGGTGCTCT ACTCCCAAAG TGGCCCCAAC
    TGCTGCGGTA GGAACTGTGG CAAGCTGCAG ACCATCTTCC TGAAGTACCC CCAGGAGGAG
    GCGATGGGGC CGCGCATCCC AGCCCAGATC CGCGTCCAGC TCTGGTTTGG GCTCTCTGTC
    AATGAGAAGG AGTTCAACCA ATACGCTGAG GGGAAGCTCT CCGTCTTTGC TGAAACCTAC
    GAGAACCAGA CCAAGCTGGC GCTGGTGGGG AACTGGGGCA CCACCGGCCT GACTTACCCC
    AAATACTCCG ATGTCACCGG CAAGATCAAG CTCCCCAAGG ACAGCTTCCG GCCCTCCACG
    GGCTGGACCT GGGCTGGTGA TTGGTTCATC TGCCCAGAGA AGACGATGCT GCACGAGGCA
    GACGCGGGGC ACCTGAGCTT CGTGGAGGAG GTGTTTGAGA ACCAGGTGCG GCTGCCGGGC
    GGGCAGTGGA TCCACATGTC CGATGCCTAC ACCGATGTGA ATGGGGAGAA GGTTCTGCCC
    AAGGATGAGA TCGAGTGTCC TCCGGGCTGG AAATGGGAAG ACGTGGAGTG GGACACTGAC
    CTCAACAGAG CCGTGGATGA GAAAGGCTGG GAGTACGGCA TCACCATCCC TCCGGACCGC
    CGGCCCAAGG CCTGGGTGCC AGCTGAGAAG ATGTACCACA CCAACCGGCG CCGGCGCTGG
    GTGCGACTGC GCAGCAGGGA CCTGGCCCAG ATGGAGACTG TCAGGAAGCA CAAGCAGGAC
    GAGCAGGACG GCGAGGGCTG GGAATACGCC TCGCTGCTGG GCTGGCGCTT CCACCTGCGG
    CCGCGTCGCA CCGATGCCTT CCGCCGCCGC CGTTGGAGAC GTAGGATGGA GCCCTTGGAG
    CGCACCGGGG CTGCTGCTGT GTTCGCCTTG GAGGGGGCCC TGGGAGGAAT GACGGACGAC
    AAAAGCGACG ATGGCAAATC CGTCTCCACG CTGAGTTTCG GCGTCAACAG ACCCACCATC
    TCCTGCATCT TCGACTATGG GAACCGCTAC CACCTGCGCT GCTACATGTA CCAGGCACGG
    GACCTGGCTG CCATGGACAA GGACAGCTTC TCGGACCCCT ATGCCATCGT TTCCTTCCTC
    CACCAAAGCC AGAAGACGGT GGTGATCAAG AACACCCTGA ACCCCACGTG GGACCAGACG
    CTCATCTTCT ACGAGATAGA GATCTTCGGA GACCCCAAGA ACGTGTCTGA CTGCCCTCCC
    AGCATCGTGG TGGAGATATA CGACCACGAC ACCTACGGCG CTGATGAGTT CATGGGGCGC
    TGCGTGTGCC GGCCCAGCCT GCAGCGCGCG CCGCGCCTCT CCTGGCACCC CGTGCTCAAA
    GGCGGCAGGA ACGTCGGCGA GCTGCTGGCT GCCTTCGAGC TCATCCAGAG GGAGAAGCCG
    GCTGTCCACC ACATCCCTGG CTTCGAGAAC GAGCTGTCCT CCGCTCTGGA TGAGTCTGAG
    GACTCTGACC TGCCCTACCC ACCGCCCCAA CGGGAACCCA ACATCTTCAT GGTCCCCCAA
    GGGATCAAAC CGGTCCTGCA GCGCACGGCC ATCGAGATCC TGGCCTGGGG GCTGAGGAAC
    CTCAAGAGCT ACCAGCTGGC CAGCGTCACC TCACCCAGCC TGCTGGTGGA GTGCGGGGGG
    CAGCTCGTGC AGTCCTGTGT CATCAAGAAC GTCAAGAAAA ACCCCAACTT TGATGTCTGT
    GTGCTCTTCA TGGAAGTGCG TCTGCCCAGC GAGAGCCTCT ACAGTCCCCC CATCATCATC
    AAAATCATCG ACAACCGTCC GTTCGGCCGC AGGCCCGTGG TGGGGCAGTG CACCATCCGC
    TCACTGGAGG AATTCTACTG TGACCCCTAT GGTGAGGAGA CGGATGGTGC CCAGGAGCAG
    GCAGATGATG TGAGCCTCAC GCCCAGGGAT GATGTCCTCA TTGACATTGA TGACAAAGAG
    CCTCTCCTTC CCACCCAGGA GGAAGAATTC ATTGACTGGT GGAGCAAATT CTACGCTTCC
    ACAGGAGAGA GGGAGAAGTG TGGCTCCTAC TTGGAGAAGG GCTTTGACAC CCTGCAGGTC
    TATGAGACGG AGCTGGAGCG CGTGGAGGCC TTCGAGCAGC TCTCTGACTT CTGCCACACC
    TTCAGGCTGT ACCGTGGCCG GGTGCAGGAT GCCAACGATG ATCCCTCAGT TGTGGGCGAG
    TTCAAGGGCT CCTTCAAAAT CTACCCTCTG CCCGATGACC CACGCGTGCC GCTGCCGCCG
    CGGCAGTTCC ACCAACTGCC AGCCAGGGGT CCCCAGGAGT GTCTCGTCAG GGTCTACATC
    ATCCGTGCCT TCGGTCTGCA GCCCAAGGAT GCCAATGGGA AGTGCGATCC GTATGTGAAG
    ATTTCAGTGG GAAAGAAATC CATAAACGAC CAAGAAAATT ACCTCCCCTC TACGCTGGAG
    CCTGTCTTTG GAAAGATGTT TGAGTTGAGC TGCACTCTGC CCCTGGAGAA GGACCTGAAG
    GTCACACTCT ATGACTACGA CCTCCTGTCC AAGGATGAGA AGATTGGGGA GACCGTCATC
    GACCTGGAGA ATCGGTTCCT GTCGAAATAT GGGGCGCGCT GTGGGCTTCC CCAGACCTAC
    TGCATCTCTG GGCCCAACCA GTGGCGAGAC CAGCTCCGTC CCTCCCAACT GCTCCACCTC
    TTCTCCCTGC AGCACCACTA CAAGGCTCCC ACCTACACGT CCGACCGGCT CATCTTCCGG
    GAGCAGGAGT ACATCCTCTC CGAGCTGGAG GATGGGAAAC CCCCCAACCC TCACCTGGGG
    CCGGCAGAGG AGCGGCTGGC CCTGCATGCA CTGCGGAAAC AAGGGCTGGT GCCGGAGCAC
    GTGGAGACCC GGCCCCTCTA CAGCCCCCTG CAGCCAGAGA TCGAGCAGGG AAAGCTACAG
    ATGTGGGTGG ACCTGTTTCC GAAATCCTTG GGTCAGCCTG GCCCCCCTTT CAACATCACA
    CCACGCAAAG CCAAGAGGTT CTACTTGCGC TGCATCATCT GGAACACCAG GGACGTCATC
    CTCGATGACC TCAGCATCAC TGGGGAGAAG ATGAGTGACA TCTATGTCAA AGGCTGGCTG
    GTTGGCCACG AGGAGAACAA GCAGAAGACA GACGTGCACT ACAGGTCGAT GGGAGGAGAA
    GGCAACTTCA ACTGGAGGTT CATCTTCCCC TTCGACTATC TCCCTGCCGA GCAGATGTGT
    TGCGTTGCAA AGAAGGAGCA TTTCTGGAGC TTGGATAAGA CGGAAAACAA GATTTCACCG
    CAGCTGATCT TCCAGATCTG GGACAATGAC AAGTTCTCCT TCGATGACTA TTTGGGCTCC
    ATCCAGATGG ATCTCAACAG GATGCCCAAG CCTGCCAAGA CTGCTGAGAA GTGCTCCTTA
    GAGCTCATAG ATGACAGCTT GTCTGCAAGC CGCTTTGTGT CCCTCTTTGA GCAGAAGACT
    GTCAAGGGCT GGTGGCCCTG CATTGCAGAG CAGGATGAGA AGAAGATCCT GGCAGGAAAA
    CTGGAAATGA CTTTGGAAAT CGTGGCTGAA CACGAGCATG AAGAGCGGCC AGCTGGCGTG
    GGTCGGGATG AGCCAAACAT GAACCCCAAG CTGGAGGACC CCAAGCGCCC AGAGACCTCC
    TTCCTGTGGT TCACCTCCCC GTACAAGACC CTGAAGTACA TCCTGTGGCG CCGGTACAAG
    TGGCTGCTCA TCCTGGCCAT CGTGCTCTTT GTTTTGCTGC TCTTCCTTGG GATCTTCATC
    TACGCCTTCC CGAACTACGC TGCTATGAAG CTGGTGAAAC CTTTCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150247
    Clone ID Related Accession (Same CDS sequence) XM_025149875.1
    Accession Version XM_025149875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGCT GCGTGCTGCT GCGCGCCCAA CTGCGCCGCC CCGATAAGGG GGACCGGCAG 
    AGCGACCCCG TGGCCAGCCT GACCTTCCGA GGGGTGAAGA AGAAGACGAA GGTCATCAAG
    AACAGCATCA ACCCCGTATG GAACGAGGGC TTTGAATGGG ATCTGAAGGG CGTTGCTTTG
    GACCTGGGAT CTGAGCTCTG TGTTGTGGTT AAGGACCATG AGACTGTGGG GAGGAATCGG
    TTTTTGGGAG AGGCCCGCGT CCCCCTACGG GATGTGCTGT CCTCCCCCAG CCTGGCGGCC
    TCCTATGATG TCCCATTGCT GGATAGCAAG AAGCAGAGCA CCGGGGCTTC CCTCATCCTA
    CAAGTGTCCT ACAGCCCTCC TCCGGGCGCT GCCCCCCTCT TCCCTCCCCC AACCCCCCCC
    GAGGCCGCCC CGGCGGCGGT GGCAGAGTCC GACACTCTCA CTGGTAATGC GGGGGCTCCA
    CGGGGGGACG CGTCCCCCAT AGCCCACCCC ACAGCGGCCA GGGACCCGCA GCAACCTCCC
    TGCCTCCCGC CCCGCGCAGA AGTGGCCACC GAGGAGGAGG GGGAGGACCA GGCGGTGACG
    GGGGACGAGA CGGAGCCCCC ATCCTCGGGG CCGCCGGGCT CGGAGCCGCC CTCGGTGCCC
    CACAGACCCC CGGCTCCCCG CGGCCGTGGG GTGAAGCGCA GGAGGAGCTC GCCCAAAAAG
    CCGCTGTCCA ACAAACCTCA GGACTTCCAG ATCCGGGTTC GGGTGATCGA GGCCCGGCAG
    CTGCCCGGCG TTAACATCCG GCCCGTGGTG AAGGTGACGG CGGCGGGGCA GACCAAGCGG
    ACCAGGATAC GCAAAGGCAA CAGCCCCCTC TTCGACGAGA CCTTCTTCTT CAACGTCTTC
    GAGTCGCCAG CCGAGCTGTT CGACGTGCCC GTCTTCATCA CGGTCCTGGA CTCGCGCTCC
    TTCCGCATGG ATGCGGTGAT CGGAGAGTTT CGGATGGACG TTGAGAGCAT CTATTCGGAG
    CCGAAACACG CGATCCTCAG GCGGTGGCTG CTGCTCTCTG ACCCGGAGGA CTTCACGGCG
    GGGGCAAAGG GGTACCTGAA GGTCAGCCTG CTTGTGCTGG GCCCCGGGGA CGAAGCTCCT
    GTGGAGAAGA AGGACGTCTC TGAAGACAAG GAAGACATTG AGGGGAACCT CCTGCGACCC
    ACGGGGGTCA CCTTGCGAGG GGCTCAGTTC TGCCTGAAGA TCTTCAAGGC TGAGGATTTG
    CCCCAGATGG ACGACGCCGT CATGGACAAC GTCCGGCAGA TCTTCGGCTT CGAAAGCAAC
    AAGAAGAACC TGGTTGACCC CTTTGTGGAA GTCAGCTTTG CGGGGAAGAC GCTCTACTCG
    AGGATCCTGG AGAAGAATGC CAACCCGCAG TGGAATCAGT GCCTGATGCT GCCGGTGATG
    TTCCCTTCCA TGTGCGAGAA GATGAGGATC CGGGTGACCG ACTGGGACCG TCTCACACAC
    AACGACGTCG TCGGCACCGC CTACCTCTGC ATGTCCAAGA TCTCTGCGCC CGGCGGGGAG
    CTGGAGGTGG ACGACGGGCT GGGCTTCCTG CCGACCTTCG GCCCCTGCTA CGTCAACCTG
    TACGGCAGCC CCCGTGAGTT CACCGGCTTC CCGGATCCGT ACGAGGCGCT CAATTTAGGA
    AAGGGCGAGG GCGTCGCGTA CCGCGGCCGG GTGCTGCTGG AGCTGGAGAC CAAGCTGGCG
    GAGCACGGGG AGCTGAAGGT GGAGGACATC TGTGCGGATG ACATCCTGCG GGTGGAGAAG
    TACCTGCGGC GGAGGAAGTA CTCCCTCTTT GCTGCCTTCT ACTCGGCCAC CATGCTGCAG
    GACGTGGACG ATGCCATCCA GTTTGAGGTC AGCATCGGCA ACTACGGCAA CAAGTTCGAC
    AACACCTGCT TGCCCTTGGC CTCCACCACG CAGTACAGCC GGGCCGTGTT CGATGGTTGC
    CAGTATTACT ACCTGCCCTG GGGCAACGTG AAGCCCGTGG TGGTGCTGTC CTCCTATTGG
    GAAGATATCA GCTACCGAAC CGACACTCAG AACCTCCTCC AGCACGCAGC AGACAGGCTG
    GAAGCCAACC TGGAGCGGGT CCACCTCGCG CTCAAGGCCA ACGGCGCGCC GGGCGAGCTG
    GAGTCCCTGG CTGCCCAGCT GATGGACGAC GTGATTGCAG ACTGCAGTGT TCCGCTGCCC
    GACGTGCTGG GGAAGGCGGC CAGCACACAC CTGGACCAGA ACCTGTACCG CTTCCGCAGC
    ACCAACCTGG AGCAGATGGT GCGGGCGGCC CTGAGGCTGA AGCACGAGGA GCTGAGCCTG
    CCGGCCGTGC TGGAGCAGGC GGAGGACTGG CTGTGCCGGC TGCGTGCCAT GGCTGAGGAG
    CCCCAGAACA GCCTCCCAGA CGTGGTGATC TGGATGCTGC GGGGTGAGAA GCGCGTTGCC
    TACGCCCGCG TGCCGGCCCG CGAGGTGCTC TACTCCCAAA GTGGCCCCAA CTGCTGCGGT
    AGGAACTGTG GCAAGCTGCA GACCATCTTC CTGAAGTACC CCCAGGAGGA GGCGATGGGG
    CCGCGCATCC CAGCCCAGAT CCGCGTCCAG CTCTGGTTTG GGCTCTCTGT CAATGAGAAG
    GAGTTCAACC AATACGCTGA GGGGAAGCTC TCCGTCTTTG CTGAAACCTA CGAGAACCAG
    ACCAAGCTGG CGCTGGTGGG GAACTGGGGC ACCACCGGCC TGACTTACCC CAAATACTCC
    GATGTCACCG GCAAGATCAA GCTCCCCAAG GACAGCTTCC GGCCCTCCAC GGGCTGGACC
    TGGGCTGGTG ATTGGTTCAT CTGCCCAGAG AAGACGATGC TGCACGAGGC AGACGCGGGG
    CACCTGAGCT TCGTGGAGGA GGTGTTTGAG AACCAGGTGC GGCTGCCGGG CGGGCAGTGG
    ATCCACATGT CCGATGCCTA CACCGATGTG AATGGGGAGA AGGTTCTGCC CAAGGATGAG
    ATCGAGTGTC CTCCGGGCTG GAAATGGGAA GACGTGGAGT GGGACACTGA CCTCAACAGA
    GCCGTGGATG AGAAAGGCTG GGAGTACGGC ATCACCATCC CTCCGGACCG CCGGCCCAAG
    GCCTGGGTGC CAGCTGAGAA GATGTACCAC ACCAACCGGC GCCGGCGCTG GGTGCGACTG
    CGCAGCAGGG ACCTGGCCCA GATGGAGACT GTCAGGAAGC ACAAGCAGGA CGAGCAGGAC
    GGCGAGGGCT GGGAATACGC CTCGCTGCTG GGCTGGCGCT TCCACCTGCG GCCGCGTCGC
    ACCGATGCCT TCCGCCGCCG CCGTTGGAGA CGTAGGATGG AGCCCTTGGA GCGCACCGGG
    GCTGCTGCTG TGTTCGCCTT GGAGGGGGCC CTGGGAGGAA TGACGGACGA CAAAAGCGAC
    GATGGCAAAT CCGTCTCCAC GCTGAGTTTC GGCGTCAACA GACCCACCAT CTCCTGCATC
    TTCGACTATG GGAACCGCTA CCACCTGCGC TGCTACATGT ACCAGGCACG GGACCTGGCT
    GCCATGGACA AGGACAGCTT CTCGGACCCC TATGCCATCG TTTCCTTCCT CCACCAAAGC
    CAGAAGACGG TGGTGATCAA GAACACCCTG AACCCCACGT GGGACCAGAC GCTCATCTTC
    TACGAGATAG AGATCTTCGG AGACCCCAAG AACGTGTCTG ACTGCCCTCC CAGCATCGTG
    GTGGAGATAT ACGACCACGA CACCTACGGC GCTGATGAGT TCATGGGGCG CTGCGTGTGC
    CGGCCCAGCC TGCAGCGCGC GCCGCGCCTC TCCTGGCACC CCGTGCTCAA AGGCGGCAGG
    AACGTCGGCG AGCTGCTGGC TGCCTTCGAG CTCATCCAGA GGGAGAAGCC GGCTGTCCAC
    CACATCCCTG GCTTCGAGAA CGAGCTGTCC TCCGCTCTGG ATGAGTCTGA GGACTCTGAC
    CTGCCCTACC CACCGCCCCA ACGGGAACCC AACATCTTCA TGGTCCCCCA AGGGATCAAA
    CCGGTCCTGC AGCGCACGGC CATCGAGATC CTGGCCTGGG GGCTGAGGAA CCTCAAGAGC
    TACCAGCTGG CCAGCGTCAC CTCACCCAGC CTGCTGGTGG AGTGCGGGGG GCAGCTCGTG
    CAGTCCTGTG TCATCAAGAA CGTCAAGAAA AACCCCAACT TTGATGTCTG TGTGCTCTTC
    ATGGAAGTGC GTCTGCCCAG CGAGAGCCTC TACAGTCCCC CCATCATCAT CAAAATCATC
    GACAACCGTC CGTTCGGCCG CAGGCCCGTG GTGGGGCAGT GCACCATCCG CTCACTGGAG
    GAATTCTACT GTGACCCCTA TGGTGAGGAG ACGGATGGTG CCCAGGAGCA GGCAGATGAT
    GTGAGCCTCA CGCCCAGGGA TGATGTCCTC ATTGACATTG ATGACAAAGA GCCTCTCCTT
    CCCACCCAGC TTGTGGAGGG CGTGACCAGC TCAGCGCTAA TTAACCCGCC AGCTTCTAGG
    TCCGAGCCTC AAGAGGAAGA ATTCATTGAC TGGTGGAGCA AATTCTACGC TTCCACAGGA
    GAGAGGGAGA AGTGTGGCTC CTACTTGGAG AAGGGCTTTG ACACCCTGCA GGTCTATGAG
    ACGGAGCTGG AGCGCGTGGA GGCCTTCGAG CAGCTCTCTG ACTTCTGCCA CACCTTCAGG
    CTGTACCGTG GCCGGGTGCA GGATGCCAAC GATGATCCCT CAGTTGTGGG CGAGTTCAAG
    GGCTCCTTCA AAATCTACCC TCTGCCCGAT GACCCACGCG TGCCGCTGCC GCCGCGGCAG
    TTCCACCAAC TGCCAGCCAG GGGTCCCCAG GAGTGTCTCG TCAGGGTCTA CATCATCCGT
    GCCTTCGGTC TGCAGCCCAA GGATGCCAAT GGGAAGTGCG ATCCGTATGT GAAGATTTCA
    GTGGGAAAGA AATCCATAAA CGACCAAGAA AATTACCTCC CCTCTACGCT GGAGCCTGTC
    TTTGGAAAGA TGTTTGAGTT GAGCTGCACT CTGCCCCTGG AGAAGGACCT GAAGGTCACA
    CTCTATGACT ACGACCTCCT GTCCAAGGAT GAGAAGATTG GGGAGACCGT CATCGACCTG
    GAGAATCGGT TCCTGTCGAA ATATGGGGCG CGCTGTGGGC TTCCCCAGAC CTACTGCATC
    TCTGGGCCCA ACCAGTGGCG AGACCAGCTC CGTCCCTCCC AACTGCTCCA CCTCTTCTCC
    CTGCAGCACC ACTACAAGGC TCCCACCTAC ACGTCCGACC GGCTCATCTT CCGGGAGCAG
    GAGTACATCC TCTCCGAGCT GGAGGATGGG AAACCCCCCA ACCCTCACCT GGGGCCGGCA
    GAGGAGCGGC TGGCCCTGCA TGCACTGCGG AAACAAGGGC TGGTGCCGGA GCACGTGGAG
    ACCCGGCCCC TCTACAGCCC CCTGCAGCCA GAGATCGAGC AGGGAAAGCT ACAGATGTGG
    GTGGACCTGT TTCCGAAATC CTTGGGTCAG CCTGGCCCCC CTTTCAACAT CACACCACGC
    AAAGCCAAGA GGTTCTACTT GCGCTGCATC ATCTGGAACA CCAGGGACGT CATCCTCGAT
    GACCTCAGCA TCACTGGGGA GAAGATGAGT GACATCTATG TCAAAGGCTG GCTGGTTGGC
    CACGAGGAGA ACAAGCAGAA GACAGACGTG CACTACAGGT CGATGGGAGG AGAAGGCAAC
    TTCAACTGGA GGTTCATCTT CCCCTTCGAC TATCTCCCTG CCGAGCAGAT GTGTTGCGTT
    GCAAAGAAGG AGCATTTCTG GAGCTTGGAT AAGACGGAAA ACAAGATTTC ACCGCAGCTG
    ATCTTCCAGA TCTGGGACAA TGACAAGTTC TCCTTCGATG ACTATTTGGG CTCCATCCAG
    ATGGATCTCA ACAGGATGCC CAAGCCTGCC AAGACTGCTG AGAAGTGCTC CTTAGAGCTC
    ATAGATGACA GCTTGTCTGC AAGCCGCTTT GTGTCCCTCT TTGAGCAGAA GACTGTCAAG
    GGCTGGTGGC CCTGCATTGC AGAGCAGGAT GAGAAGAAGA TCCTGGCAGG AAAACTGGAA
    ATGACTTTGG AAATCGTGGC TGAACACGAG CATGAAGAGC GGCCAGCTGG CGTGGGTCGG
    GATGAGCCAA ACATGAACCC CAAGCTGGAG GACCCCAAGC GCCCAGAGAC CTCCTTCCTG
    TGGTTCACCT CCCCGTACAA GACCCTGAAG TACATCCTGT GGCGCCGGTA CAAGTGGCTG
    CTCATCCTGG CCATCGTGCT CTTTGTTTTG CTGCTCTTCC TTGGGATCTT CATCTACGCC
    TTCCCGAACT ACGCTGCTAT GAAGCTGGTG AAACCTTTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005643.1
    CDS36..6380
    Translation

    Target ORF information:

    RefSeq Version XM_025149875.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGCT GCGTGCTGCT GCGCGCCCAA CTGCGCCGCC CCGATAAGGG GGACCGGCAG 
    AGCGACCCCG TGGCCAGCCT GACCTTCCGA GGGGTGAAGA AGAAGACGAA GGTCATCAAG
    AACAGCATCA ACCCCGTATG GAACGAGGGC TTTGAATGGG ATCTGAAGGG CGTTGCTTTG
    GACCTGGGAT CTGAGCTCTG TGTTGTGGTT AAGGACCATG AGACTGTGGG GAGGAATCGG
    TTTTTGGGAG AGGCCCGCGT CCCCCTACGG GATGTGCTGT CCTCCCCCAG CCTGGCGGCC
    TCCTATGATG TCCCATTGCT GGATAGCAAG AAGCAGAGCA CCGGGGCTTC CCTCATCCTA
    CAAGTGTCCT ACAGCCCTCC TCCGGGCGCT GCCCCCCTCT TCCCTCCCCC AACCCCCCCC
    GAGGCCGCCC CGGCGGCGGT GGCAGAGTCC GACACTCTCA CTGGTAATGC GGGGGCTCCA
    CGGGGGGACG CGTCCCCCAT AGCCCACCCC ACAGCGGCCA GGGACCCGCA GCAACCTCCC
    TGCCTCCCGC CCCGCGCAGA AGTGGCCACC GAGGAGGAGG GGGAGGACCA GGCGGTGACG
    GGGGACGAGA CGGAGCCCCC ATCCTCGGGG CCGCCGGGCT CGGAGCCGCC CTCGGTGCCC
    CACAGACCCC CGGCTCCCCG CGGCCGTGGG GTGAAGCGCA GGAGGAGCTC GCCCAAAAAG
    CCGCTGTCCA ACAAACCTCA GGACTTCCAG ATCCGGGTTC GGGTGATCGA GGCCCGGCAG
    CTGCCCGGCG TTAACATCCG GCCCGTGGTG AAGGTGACGG CGGCGGGGCA GACCAAGCGG
    ACCAGGATAC GCAAAGGCAA CAGCCCCCTC TTCGACGAGA CCTTCTTCTT CAACGTCTTC
    GAGTCGCCAG CCGAGCTGTT CGACGTGCCC GTCTTCATCA CGGTCCTGGA CTCGCGCTCC
    TTCCGCATGG ATGCGGTGAT CGGAGAGTTT CGGATGGACG TTGAGAGCAT CTATTCGGAG
    CCGAAACACG CGATCCTCAG GCGGTGGCTG CTGCTCTCTG ACCCGGAGGA CTTCACGGCG
    GGGGCAAAGG GGTACCTGAA GGTCAGCCTG CTTGTGCTGG GCCCCGGGGA CGAAGCTCCT
    GTGGAGAAGA AGGACGTCTC TGAAGACAAG GAAGACATTG AGGGGAACCT CCTGCGACCC
    ACGGGGGTCA CCTTGCGAGG GGCTCAGTTC TGCCTGAAGA TCTTCAAGGC TGAGGATTTG
    CCCCAGATGG ACGACGCCGT CATGGACAAC GTCCGGCAGA TCTTCGGCTT CGAAAGCAAC
    AAGAAGAACC TGGTTGACCC CTTTGTGGAA GTCAGCTTTG CGGGGAAGAC GCTCTACTCG
    AGGATCCTGG AGAAGAATGC CAACCCGCAG TGGAATCAGT GCCTGATGCT GCCGGTGATG
    TTCCCTTCCA TGTGCGAGAA GATGAGGATC CGGGTGACCG ACTGGGACCG TCTCACACAC
    AACGACGTCG TCGGCACCGC CTACCTCTGC ATGTCCAAGA TCTCTGCGCC CGGCGGGGAG
    CTGGAGGTGG ACGACGGGCT GGGCTTCCTG CCGACCTTCG GCCCCTGCTA CGTCAACCTG
    TACGGCAGCC CCCGTGAGTT CACCGGCTTC CCGGATCCGT ACGAGGCGCT CAATTTAGGA
    AAGGGCGAGG GCGTCGCGTA CCGCGGCCGG GTGCTGCTGG AGCTGGAGAC CAAGCTGGCG
    GAGCACGGGG AGCTGAAGGT GGAGGACATC TGTGCGGATG ACATCCTGCG GGTGGAGAAG
    TACCTGCGGC GGAGGAAGTA CTCCCTCTTT GCTGCCTTCT ACTCGGCCAC CATGCTGCAG
    GACGTGGACG ATGCCATCCA GTTTGAGGTC AGCATCGGCA ACTACGGCAA CAAGTTCGAC
    AACACCTGCT TGCCCTTGGC CTCCACCACG CAGTACAGCC GGGCCGTGTT CGATGGTTGC
    CAGTATTACT ACCTGCCCTG GGGCAACGTG AAGCCCGTGG TGGTGCTGTC CTCCTATTGG
    GAAGATATCA GCTACCGAAC CGACACTCAG AACCTCCTCC AGCACGCAGC AGACAGGCTG
    GAAGCCAACC TGGAGCGGGT CCACCTCGCG CTCAAGGCCA ACGGCGCGCC GGGCGAGCTG
    GAGTCCCTGG CTGCCCAGCT GATGGACGAC GTGATTGCAG ACTGCAGTGT TCCGCTGCCC
    GACGTGCTGG GGAAGGCGGC CAGCACACAC CTGGACCAGA ACCTGTACCG CTTCCGCAGC
    ACCAACCTGG AGCAGATGGT GCGGGCGGCC CTGAGGCTGA AGCACGAGGA GCTGAGCCTG
    CCGGCCGTGC TGGAGCAGGC GGAGGACTGG CTGTGCCGGC TGCGTGCCAT GGCTGAGGAG
    CCCCAGAACA GCCTCCCAGA CGTGGTGATC TGGATGCTGC GGGGTGAGAA GCGCGTTGCC
    TACGCCCGCG TGCCGGCCCG CGAGGTGCTC TACTCCCAAA GTGGCCCCAA CTGCTGCGGT
    AGGAACTGTG GCAAGCTGCA GACCATCTTC CTGAAGTACC CCCAGGAGGA GGCGATGGGG
    CCGCGCATCC CAGCCCAGAT CCGCGTCCAG CTCTGGTTTG GGCTCTCTGT CAATGAGAAG
    GAGTTCAACC AATACGCTGA GGGGAAGCTC TCCGTCTTTG CTGAAACCTA CGAGAACCAG
    ACCAAGCTGG CGCTGGTGGG GAACTGGGGC ACCACCGGCC TGACTTACCC CAAATACTCC
    GATGTCACCG GCAAGATCAA GCTCCCCAAG GACAGCTTCC GGCCCTCCAC GGGCTGGACC
    TGGGCTGGTG ATTGGTTCAT CTGCCCAGAG AAGACGATGC TGCACGAGGC AGACGCGGGG
    CACCTGAGCT TCGTGGAGGA GGTGTTTGAG AACCAGGTGC GGCTGCCGGG CGGGCAGTGG
    ATCCACATGT CCGATGCCTA CACCGATGTG AATGGGGAGA AGGTTCTGCC CAAGGATGAG
    ATCGAGTGTC CTCCGGGCTG GAAATGGGAA GACGTGGAGT GGGACACTGA CCTCAACAGA
    GCCGTGGATG AGAAAGGCTG GGAGTACGGC ATCACCATCC CTCCGGACCG CCGGCCCAAG
    GCCTGGGTGC CAGCTGAGAA GATGTACCAC ACCAACCGGC GCCGGCGCTG GGTGCGACTG
    CGCAGCAGGG ACCTGGCCCA GATGGAGACT GTCAGGAAGC ACAAGCAGGA CGAGCAGGAC
    GGCGAGGGCT GGGAATACGC CTCGCTGCTG GGCTGGCGCT TCCACCTGCG GCCGCGTCGC
    ACCGATGCCT TCCGCCGCCG CCGTTGGAGA CGTAGGATGG AGCCCTTGGA GCGCACCGGG
    GCTGCTGCTG TGTTCGCCTT GGAGGGGGCC CTGGGAGGAA TGACGGACGA CAAAAGCGAC
    GATGGCAAAT CCGTCTCCAC GCTGAGTTTC GGCGTCAACA GACCCACCAT CTCCTGCATC
    TTCGACTATG GGAACCGCTA CCACCTGCGC TGCTACATGT ACCAGGCACG GGACCTGGCT
    GCCATGGACA AGGACAGCTT CTCGGACCCC TATGCCATCG TTTCCTTCCT CCACCAAAGC
    CAGAAGACGG TGGTGATCAA GAACACCCTG AACCCCACGT GGGACCAGAC GCTCATCTTC
    TACGAGATAG AGATCTTCGG AGACCCCAAG AACGTGTCTG ACTGCCCTCC CAGCATCGTG
    GTGGAGATAT ACGACCACGA CACCTACGGC GCTGATGAGT TCATGGGGCG CTGCGTGTGC
    CGGCCCAGCC TGCAGCGCGC GCCGCGCCTC TCCTGGCACC CCGTGCTCAA AGGCGGCAGG
    AACGTCGGCG AGCTGCTGGC TGCCTTCGAG CTCATCCAGA GGGAGAAGCC GGCTGTCCAC
    CACATCCCTG GCTTCGAGAA CGAGCTGTCC TCCGCTCTGG ATGAGTCTGA GGACTCTGAC
    CTGCCCTACC CACCGCCCCA ACGGGAACCC AACATCTTCA TGGTCCCCCA AGGGATCAAA
    CCGGTCCTGC AGCGCACGGC CATCGAGATC CTGGCCTGGG GGCTGAGGAA CCTCAAGAGC
    TACCAGCTGG CCAGCGTCAC CTCACCCAGC CTGCTGGTGG AGTGCGGGGG GCAGCTCGTG
    CAGTCCTGTG TCATCAAGAA CGTCAAGAAA AACCCCAACT TTGATGTCTG TGTGCTCTTC
    ATGGAAGTGC GTCTGCCCAG CGAGAGCCTC TACAGTCCCC CCATCATCAT CAAAATCATC
    GACAACCGTC CGTTCGGCCG CAGGCCCGTG GTGGGGCAGT GCACCATCCG CTCACTGGAG
    GAATTCTACT GTGACCCCTA TGGTGAGGAG ACGGATGGTG CCCAGGAGCA GGCAGATGAT
    GTGAGCCTCA CGCCCAGGGA TGATGTCCTC ATTGACATTG ATGACAAAGA GCCTCTCCTT
    CCCACCCAGC TTGTGGAGGG CGTGACCAGC TCAGCGCTAA TTAACCCGCC AGCTTCTAGG
    TCCGAGCCTC AAGAGGAAGA ATTCATTGAC TGGTGGAGCA AATTCTACGC TTCCACAGGA
    GAGAGGGAGA AGTGTGGCTC CTACTTGGAG AAGGGCTTTG ACACCCTGCA GGTCTATGAG
    ACGGAGCTGG AGCGCGTGGA GGCCTTCGAG CAGCTCTCTG ACTTCTGCCA CACCTTCAGG
    CTGTACCGTG GCCGGGTGCA GGATGCCAAC GATGATCCCT CAGTTGTGGG CGAGTTCAAG
    GGCTCCTTCA AAATCTACCC TCTGCCCGAT GACCCACGCG TGCCGCTGCC GCCGCGGCAG
    TTCCACCAAC TGCCAGCCAG GGGTCCCCAG GAGTGTCTCG TCAGGGTCTA CATCATCCGT
    GCCTTCGGTC TGCAGCCCAA GGATGCCAAT GGGAAGTGCG ATCCGTATGT GAAGATTTCA
    GTGGGAAAGA AATCCATAAA CGACCAAGAA AATTACCTCC CCTCTACGCT GGAGCCTGTC
    TTTGGAAAGA TGTTTGAGTT GAGCTGCACT CTGCCCCTGG AGAAGGACCT GAAGGTCACA
    CTCTATGACT ACGACCTCCT GTCCAAGGAT GAGAAGATTG GGGAGACCGT CATCGACCTG
    GAGAATCGGT TCCTGTCGAA ATATGGGGCG CGCTGTGGGC TTCCCCAGAC CTACTGCATC
    TCTGGGCCCA ACCAGTGGCG AGACCAGCTC CGTCCCTCCC AACTGCTCCA CCTCTTCTCC
    CTGCAGCACC ACTACAAGGC TCCCACCTAC ACGTCCGACC GGCTCATCTT CCGGGAGCAG
    GAGTACATCC TCTCCGAGCT GGAGGATGGG AAACCCCCCA ACCCTCACCT GGGGCCGGCA
    GAGGAGCGGC TGGCCCTGCA TGCACTGCGG AAACAAGGGC TGGTGCCGGA GCACGTGGAG
    ACCCGGCCCC TCTACAGCCC CCTGCAGCCA GAGATCGAGC AGGGAAAGCT ACAGATGTGG
    GTGGACCTGT TTCCGAAATC CTTGGGTCAG CCTGGCCCCC CTTTCAACAT CACACCACGC
    AAAGCCAAGA GGTTCTACTT GCGCTGCATC ATCTGGAACA CCAGGGACGT CATCCTCGAT
    GACCTCAGCA TCACTGGGGA GAAGATGAGT GACATCTATG TCAAAGGCTG GCTGGTTGGC
    CACGAGGAGA ACAAGCAGAA GACAGACGTG CACTACAGGT CGATGGGAGG AGAAGGCAAC
    TTCAACTGGA GGTTCATCTT CCCCTTCGAC TATCTCCCTG CCGAGCAGAT GTGTTGCGTT
    GCAAAGAAGG AGCATTTCTG GAGCTTGGAT AAGACGGAAA ACAAGATTTC ACCGCAGCTG
    ATCTTCCAGA TCTGGGACAA TGACAAGTTC TCCTTCGATG ACTATTTGGG CTCCATCCAG
    ATGGATCTCA ACAGGATGCC CAAGCCTGCC AAGACTGCTG AGAAGTGCTC CTTAGAGCTC
    ATAGATGACA GCTTGTCTGC AAGCCGCTTT GTGTCCCTCT TTGAGCAGAA GACTGTCAAG
    GGCTGGTGGC CCTGCATTGC AGAGCAGGAT GAGAAGAAGA TCCTGGCAGG AAAACTGGAA
    ATGACTTTGG AAATCGTGGC TGAACACGAG CATGAAGAGC GGCCAGCTGG CGTGGGTCGG
    GATGAGCCAA ACATGAACCC CAAGCTGGAG GACCCCAAGC GCCCAGAGAC CTCCTTCCTG
    TGGTTCACCT CCCCGTACAA GACCCTGAAG TACATCCTGT GGCGCCGGTA CAAGTGGCTG
    CTCATCCTGG CCATCGTGCT CTTTGTTTTG CTGCTCTTCC TTGGGATCTT CATCTACGCC
    TTCCCGAACT ACGCTGCTAT GAAGCTGGTG AAACCTTTCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150248
    Clone ID Related Accession (Same CDS sequence) XM_025149876.1
    Accession Version XM_025149876.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGCT GCGTGCTGCT GCGCGCCCAA CTGCGCCGCC CCGATAAGGG GGACCGGCAG 
    AGCGACCCCG TGGCCAGCCT GACCTTCCGA GGGGTGAAGA AGAAGACGAA GGTCATCAAG
    AACAGCATCA ACCCCGTATG GAACGAGGGC TTTGAATGGG ATCTGAAGGG CGTTGCTTTG
    GACCTGGGAT CTGAGCTCTG TGTTGTGGTT AAGGACCATG AGACTGTGGG GAGGAATCGG
    TTTTTGGGAG AGGCCCGCGT CCCCCTACGG GATGTGCTGT CCTCCCCCAG CCTGGCGGCC
    TCCTATGATG TCCCATTGCT GGATAGCAAG AAGCAGAGCA CCGGGGCTTC CCTCATCCTA
    CAAGTGTCCT ACAGCCCTCC TCCGGGCGCT GCCCCCCTCT TCCCTCCCCC AACCCCCCCC
    GAGGCCGCCC CGGCGGCGGT GGCAGAGTCC GACACTCTCA CTGGTAATGC GGGGGCTCCA
    CGGGGGGACG CGTCCCCCAT AGCCCACCCC ACAGCGGCCA GGGACCCGCA GCAACCTCCC
    TGCCTCCCGC CCCGCGCAGA AGTGGCCACC GAGGAGGAGG GGGAGGACCA GGCGGTGACG
    GGGGACGAGA CGGAGCCCCC ATCCTCGGGG CCGCCGGGCT CGGAGCCGCC CTCGGTGCCC
    CACAGACCCC CGGCTCCCCG CGGCCGTGGG GTGAAGCGCA GGAGGAGCTC GCCCAAAAAG
    CCGCTGTCCA ACAAACCTCA GGACTTCCAG ATCCGGGTTC GGGTGATCGA GGCCCGGCAG
    CTGCCCGGCG TTAACATCCG GCCCGTGGTG AAGGTGACGG CGGCGGGGCA GACCAAGCGG
    ACCAGGATAC GCAAAGGCAA CAGCCCCCTC TTCGACGAGA CCTTCTTCTT CAACGTCTTC
    GAGTCGCCAG CCGAGCTGTT CGACGTGCCC GTCTTCATCA CGGTCCTGGA CTCGCGCTCC
    TTCCGCATGG ATGCGGTGAT CGGAGAGTTT CGGATGGACG TTGAGAGCAT CTATTCGGAG
    CCGAAACACG CGATCCTCAG GCGGTGGCTG CTGCTCTCTG ACCCGGAGGA CTTCACGGCG
    GGGGCAAAGG GGTACCTGAA GGTCAGCCTG CTTGTGCTGG GCCCCGGGGA CGAAGCTCCT
    GTGGAGAAGA AGGACGTCTC TGAAGACAAG GAAGACATTG AGGGGAACCT CCTGCGACCC
    ACGGGGGTCA CCTTGCGAGG GGCTCAGTTC TGCCTGAAGA TCTTCAAGGC TGAGGATTTG
    CCCCAGATGG ACGACGCCGT CATGGACAAC GTCCGGCAGA TCTTCGGCTT CGAAAGCAAC
    AAGAAGAACC TGGTTGACCC CTTTGTGGAA GTCAGCTTTG CGGGGAAGAC GCTCTACTCG
    AGGATCCTGG AGAAGAATGC CAACCCGCAG TGGAATCAGT GCCTGATGCT GCCGGTGATG
    TTCCCTTCCA TGTGCGAGAA GATGAGGATC CGGGTGACCG ACTGGGACCG TCTCACACAC
    AACGACGTCG TCGGCACCGC CTACCTCTGC ATGTCCAAGA TCTCTGCGCC CGGCGGGGAG
    CTGGAGGTGG ACGACGGGCT GGGCTTCCTG CCGACCTTCG GCCCCTGCTA CGTCAACCTG
    TACGGCAGCC CCCGTGAGTT CACCGGCTTC CCGGATCCGT ACGAGGCGCT CAATTTAGGA
    AAGGGCGAGG GCGTCGCGTA CCGCGGCCGG GTGCTGCTGG AGCTGGAGAC CAAGCTGGCG
    GAGCACGGGG AGCTGAAGGT GGAGGACATC TGTGCGGATG ACATCCTGCG GGTGGAGAAG
    TACCTGCGGC GGAGGAAGTA CTCCCTCTTT GCTGCCTTCT ACTCGGCCAC CATGCTGCAG
    GACGTGGACG ATGCCATCCA GTTTGAGGTC AGCATCGGCA ACTACGGCAA CAAGTTCGAC
    AACACCTGCT TGCCCTTGGC CTCCACCACG CAGTACAGCC GGGCCGTGTT CGATGGTTGC
    CAGTATTACT ACCTGCCCTG GGGCAACGTG AAGCCCGTGG TGGTGCTGTC CTCCTATTGG
    GAAGATATCA GCTACCGAAC CGACACTCAG AACCTCCTCC AGCACGCAGC AGACAGGCTG
    GAAGCCAACC TGGAGCGGGT CCACCTCGCG CTCAAGGCCA ACGGCGCGCC GGGCGAGCTG
    GAGTCCCTGG CTGCCCAGCT GATGGACGAC GTGATTGCAG ACTGCAGTGT TCCGCTGCCC
    GACGTGCTGG GGAAGGCGGC CAGCACACAC CTGGACCAGA ACCTGTACCG CTTCCGCAGC
    ACCAACCTGG AGCAGATGGT GCGGGCGGCC CTGAGGCTGA AGCACGAGGA GCTGAGCCTG
    CCGGCCGTGC TGGAGCAGGC GGAGGACTGG CTGTGCCGGC TGCGTGCCAT GGCTGAGGAG
    CCCCAGAACA GCCTCCCAGA CGTGGTGATC TGGATGCTGC GGGGTGAGAA GCGCGTTGCC
    TACGCCCGCG TGCCGGCCCG CGAGGTGCTC TACTCCCAAA GTGGCCCCAA CTGCTGCGGT
    AGGAACTGTG GCAAGCTGCA GACCATCTTC CTGAAGTACC CCCAGGAGGA GGCGATGGGG
    CCGCGCATCC CAGCCCAGAT CCGCGTCCAG CTCTGGTTTG GGCTCTCTGT CAATGAGAAG
    GAGTTCAACC AATACGCTGA GGGGAAGCTC TCCGTCTTTG CTGAAACCTA CGAGAACCAG
    ACCAAGCTGG CGCTGGTGGG GAACTGGGGC ACCACCGGCC TGACTTACCC CAAATACTCC
    GATGTCACCG GCAAGATCAA GCTCCCCAAG GACAGCTTCC GGCCCTCCAC GGGCTGGACC
    TGGGCTGGTG ATTGGTTCAT CTGCCCAGAG AAGACGATGC TGCACGAGGC AGACGCGGGG
    CACCTGAGCT TCGTGGAGGA GGTGTTTGAG AACCAGGTGC GGCTGCCGGG CGGGCAGTGG
    ATCCACATGT CCGATGCCTA CACCGATGTG AATGGGGAGA AGGTTCTGCC CAAGGATGAG
    ATCGAGTGTC CTCCGGGCTG GAAATGGGAA GACGTGGAGT GGGACACTGA CCTCAACAGA
    GCCGTGGATG AGAAAGGCTG GGAGTACGGC ATCACCATCC CTCCGGACCG CCGGCCCAAG
    GCCTGGGTGC CAGCTGAGAA GATGTACCAC ACCAACCGGC GCCGGCGCTG GGTGCGACTG
    CGCAGCAGGG ACCTGGCCCA GATGGAGACT GTCAGGAAGC ACAAGCAGGA CGAGCAGGAC
    GGCGAGGGCT GGGAATACGC CTCGCTGCTG GGCTGGCGCT TCCACCTGCG GCCGCGTCGC
    ACCGATGCCT TCCGCCGCCG CCGTTGGAGA CGTAGGATGG AGCCCTTGGA GCGCACCGGG
    GCTGCTGCTG TGTTCGCCTT GGAGGGGGCC CTGGGAGGAA TGACGGACGA CAAAAGCGAC
    GATGGCAAAT CCGTCTCCAC GCTGAGTTTC GGCGTCAACA GACCCACCAT CTCCTGCATC
    TTCGACTATG GGAACCGCTA CCACCTGCGC TGCTACATGT ACCAGGCACG GGACCTGGCT
    GCCATGGACA AGGACAGCTT CTCGGACCCC TATGCCATCG TTTCCTTCCT CCACCAAAGC
    CAGAAGACGG TGGTGATCAA GAACACCCTG AACCCCACGT GGGACCAGAC GCTCATCTTC
    TACGAGATAG AGATCTTCGG AGACCCCAAG AACGTGTCTG ACTGCCCTCC CAGCATCGTG
    GTGGAGATAT ACGACCACGA CACCTACGGC GCTGATGAGT TCATGGGGCG CTGCGTGTGC
    CGGCCCAGCC TGCAGCGCGC GCCGCGCCTC TCCTGGCACC CCGTGCTCAA AGGCGGCAGG
    AACGTCGGCG AGCTGCTGGC TGCCTTCGAG CTCATCCAGA GGGAGAAGCC GGCTGTCCAC
    CACATCCCTG GCTTCGAGAA CGAGCTGTCC TCCGCTCTGG ATGAGTCTGA GGACTCTGAC
    CTGCCCTACC CACCGCCCCA ACGGGAACCC AACATCTTCA TGGTCCCCCA AGGGATCAAA
    CCGGTCCTGC AGCGCACGGC CATCGAGATC CTGGCCTGGG GGCTGAGGAA CCTCAAGAGC
    TACCAGCTGG CCAGCGTCAC CTCACCCAGC CTGCTGGTGG AGTGCGGGGG GCAGCTCGTG
    CAGTCCTGTG TCATCAAGAA CGTCAAGAAA AACCCCAACT TTGATGTCTG TGTGCTCTTC
    ATGGAAGTGC GTCTGCCCAG CGAGAGCCTC TACAGTCCCC CCATCATCAT CAAAATCATC
    GACAACCGTC CGTTCGGCCG CAGGCCCGTG GTGGGGCAGT GCACCATCCG CTCACTGGAG
    GAATTCTACT GTGACCCCTA TGGTGAGGAG ACGGATGGTG CCCAGGAGCA GGCAGATGAT
    GTGAGCCTCA CGCCCAGGGA TGATGTCCTC ATTGACATTG ATGACAAAGA GCCTCTCCTT
    CCCACCCAGG AGGAAGAATT CATTGACTGG TGGAGCAAAT TCTACGCTTC CACAGGAGAG
    AGGGAGAAGT GTGGCTCCTA CTTGGAGAAG GGCTTTGACA CCCTGCAGGT CTATGAGACG
    GAGCTGGAGC GCGTGGAGGC CTTCGAGCAG CTCTCTGACT TCTGCCACAC CTTCAGGCTG
    TACCGTGGCC GGGTGCAGGA TGCCAACGAT GATCCCTCAG TTGTGGGCGA GTTCAAGGGC
    TCCTTCAAAA TCTACCCTCT GCCCGATGAC CCACGCGTGC CGCTGCCGCC GCGGCAGTTC
    CACCAACTGC CAGCCAGGGG TCCCCAGGAG TGTCTCGTCA GGGTCTACAT CATCCGTGCC
    TTCGGTCTGC AGCCCAAGGA TGCCAATGGG AAGTGCGATC CGTATGTGAA GATTTCAGTG
    GGAAAGAAAT CCATAAACGA CCAAGAAAAT TACCTCCCCT CTACGCTGGA GCCTGTCTTT
    GGAAAGATGT TTGAGTTGAG CTGCACTCTG CCCCTGGAGA AGGACCTGAA GGTCACACTC
    TATGACTACG ACCTCCTGTC CAAGGATGAG AAGATTGGGG AGACCGTCAT CGACCTGGAG
    AATCGGTTCC TGTCGAAATA TGGGGCGCGC TGTGGGCTTC CCCAGACCTA CTGCATCTCT
    GGGCCCAACC AGTGGCGAGA CCAGCTCCGT CCCTCCCAAC TGCTCCACCT CTTCTCCCTG
    CAGCACCACT ACAAGGCTCC CACCTACACG TCCGACCGGC TCATCTTCCG GGAGCAGGAG
    TACATCCTCT CCGAGCTGGA GGATGGGAAA CCCCCCAACC CTCACCTGGG GCCGGCAGAG
    GAGCGGCTGG CCCTGCATGC ACTGCGGAAA CAAGGGCTGG TGCCGGAGCA CGTGGAGACC
    CGGCCCCTCT ACAGCCCCCT GCAGCCAGAG ATCGAGCAGG GAAAGCTACA GATGTGGGTG
    GACCTGTTTC CGAAATCCTT GGGTCAGCCT GGCCCCCCTT TCAACATCAC ACCACGCAAA
    GCCAAGAGGT TCTACTTGCG CTGCATCATC TGGAACACCA GGGACGTCAT CCTCGATGAC
    CTCAGCATCA CTGGGGAGAA GATGAGTGAC ATCTATGTCA AAGGCTGGCT GGTTGGCCAC
    GAGGAGAACA AGCAGAAGAC AGACGTGCAC TACAGGTCGA TGGGAGGAGA AGGCAACTTC
    AACTGGAGGT TCATCTTCCC CTTCGACTAT CTCCCTGCCG AGCAGATGTG TTGCGTTGCA
    AAGAAGGAGC ATTTCTGGAG CTTGGATAAG ACGGAAAACA AGATTTCACC GCAGCTGATC
    TTCCAGATCT GGGACAATGA CAAGTTCTCC TTCGATGACT ATTTGGGCTC CATCCAGATG
    GATCTCAACA GGATGCCCAA GCCTGCCAAG ACTGCTGAGA AGTGCTCCTT AGAGCTCATA
    GATGACAGCT TGTCTGCAAG CCGCTTTGTG TCCCTCTTTG AGCAGAAGAC TGTCAAGGGC
    TGGTGGCCCT GCATTGCAGA GCAGGATGAG AAGAAGATCC TGGCAGGAAA ACTGGAAATG
    ACTTTGGAAA TCGTGGCTGA ACACGAGCAT GAAGAGCGGC CAGCTGGCGT GGGTCGGGAT
    GAGCCAAACA TGAACCCCAA GCTGGAGGAC CCCAAGCGCC CAGAGACCTC CTTCCTGTGG
    TTCACCTCCC CGTACAAGAC CCTGAAGTAC ATCCTGTGGC GCCGGTACAA GTGGCTGCTC
    ATCCTGGCCA TCGTGCTCTT TGTTTTGCTG CTCTTCCTTG GGATCTTCAT CTACGCCTTC
    CCGAACTACG CTGCTATGAA GCTGGTGAAA CCTTTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005644.1
    CDS36..6317
    Translation

    Target ORF information:

    RefSeq Version XM_025149876.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149876.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGCT GCGTGCTGCT GCGCGCCCAA CTGCGCCGCC CCGATAAGGG GGACCGGCAG 
    AGCGACCCCG TGGCCAGCCT GACCTTCCGA GGGGTGAAGA AGAAGACGAA GGTCATCAAG
    AACAGCATCA ACCCCGTATG GAACGAGGGC TTTGAATGGG ATCTGAAGGG CGTTGCTTTG
    GACCTGGGAT CTGAGCTCTG TGTTGTGGTT AAGGACCATG AGACTGTGGG GAGGAATCGG
    TTTTTGGGAG AGGCCCGCGT CCCCCTACGG GATGTGCTGT CCTCCCCCAG CCTGGCGGCC
    TCCTATGATG TCCCATTGCT GGATAGCAAG AAGCAGAGCA CCGGGGCTTC CCTCATCCTA
    CAAGTGTCCT ACAGCCCTCC TCCGGGCGCT GCCCCCCTCT TCCCTCCCCC AACCCCCCCC
    GAGGCCGCCC CGGCGGCGGT GGCAGAGTCC GACACTCTCA CTGGTAATGC GGGGGCTCCA
    CGGGGGGACG CGTCCCCCAT AGCCCACCCC ACAGCGGCCA GGGACCCGCA GCAACCTCCC
    TGCCTCCCGC CCCGCGCAGA AGTGGCCACC GAGGAGGAGG GGGAGGACCA GGCGGTGACG
    GGGGACGAGA CGGAGCCCCC ATCCTCGGGG CCGCCGGGCT CGGAGCCGCC CTCGGTGCCC
    CACAGACCCC CGGCTCCCCG CGGCCGTGGG GTGAAGCGCA GGAGGAGCTC GCCCAAAAAG
    CCGCTGTCCA ACAAACCTCA GGACTTCCAG ATCCGGGTTC GGGTGATCGA GGCCCGGCAG
    CTGCCCGGCG TTAACATCCG GCCCGTGGTG AAGGTGACGG CGGCGGGGCA GACCAAGCGG
    ACCAGGATAC GCAAAGGCAA CAGCCCCCTC TTCGACGAGA CCTTCTTCTT CAACGTCTTC
    GAGTCGCCAG CCGAGCTGTT CGACGTGCCC GTCTTCATCA CGGTCCTGGA CTCGCGCTCC
    TTCCGCATGG ATGCGGTGAT CGGAGAGTTT CGGATGGACG TTGAGAGCAT CTATTCGGAG
    CCGAAACACG CGATCCTCAG GCGGTGGCTG CTGCTCTCTG ACCCGGAGGA CTTCACGGCG
    GGGGCAAAGG GGTACCTGAA GGTCAGCCTG CTTGTGCTGG GCCCCGGGGA CGAAGCTCCT
    GTGGAGAAGA AGGACGTCTC TGAAGACAAG GAAGACATTG AGGGGAACCT CCTGCGACCC
    ACGGGGGTCA CCTTGCGAGG GGCTCAGTTC TGCCTGAAGA TCTTCAAGGC TGAGGATTTG
    CCCCAGATGG ACGACGCCGT CATGGACAAC GTCCGGCAGA TCTTCGGCTT CGAAAGCAAC
    AAGAAGAACC TGGTTGACCC CTTTGTGGAA GTCAGCTTTG CGGGGAAGAC GCTCTACTCG
    AGGATCCTGG AGAAGAATGC CAACCCGCAG TGGAATCAGT GCCTGATGCT GCCGGTGATG
    TTCCCTTCCA TGTGCGAGAA GATGAGGATC CGGGTGACCG ACTGGGACCG TCTCACACAC
    AACGACGTCG TCGGCACCGC CTACCTCTGC ATGTCCAAGA TCTCTGCGCC CGGCGGGGAG
    CTGGAGGTGG ACGACGGGCT GGGCTTCCTG CCGACCTTCG GCCCCTGCTA CGTCAACCTG
    TACGGCAGCC CCCGTGAGTT CACCGGCTTC CCGGATCCGT ACGAGGCGCT CAATTTAGGA
    AAGGGCGAGG GCGTCGCGTA CCGCGGCCGG GTGCTGCTGG AGCTGGAGAC CAAGCTGGCG
    GAGCACGGGG AGCTGAAGGT GGAGGACATC TGTGCGGATG ACATCCTGCG GGTGGAGAAG
    TACCTGCGGC GGAGGAAGTA CTCCCTCTTT GCTGCCTTCT ACTCGGCCAC CATGCTGCAG
    GACGTGGACG ATGCCATCCA GTTTGAGGTC AGCATCGGCA ACTACGGCAA CAAGTTCGAC
    AACACCTGCT TGCCCTTGGC CTCCACCACG CAGTACAGCC GGGCCGTGTT CGATGGTTGC
    CAGTATTACT ACCTGCCCTG GGGCAACGTG AAGCCCGTGG TGGTGCTGTC CTCCTATTGG
    GAAGATATCA GCTACCGAAC CGACACTCAG AACCTCCTCC AGCACGCAGC AGACAGGCTG
    GAAGCCAACC TGGAGCGGGT CCACCTCGCG CTCAAGGCCA ACGGCGCGCC GGGCGAGCTG
    GAGTCCCTGG CTGCCCAGCT GATGGACGAC GTGATTGCAG ACTGCAGTGT TCCGCTGCCC
    GACGTGCTGG GGAAGGCGGC CAGCACACAC CTGGACCAGA ACCTGTACCG CTTCCGCAGC
    ACCAACCTGG AGCAGATGGT GCGGGCGGCC CTGAGGCTGA AGCACGAGGA GCTGAGCCTG
    CCGGCCGTGC TGGAGCAGGC GGAGGACTGG CTGTGCCGGC TGCGTGCCAT GGCTGAGGAG
    CCCCAGAACA GCCTCCCAGA CGTGGTGATC TGGATGCTGC GGGGTGAGAA GCGCGTTGCC
    TACGCCCGCG TGCCGGCCCG CGAGGTGCTC TACTCCCAAA GTGGCCCCAA CTGCTGCGGT
    AGGAACTGTG GCAAGCTGCA GACCATCTTC CTGAAGTACC CCCAGGAGGA GGCGATGGGG
    CCGCGCATCC CAGCCCAGAT CCGCGTCCAG CTCTGGTTTG GGCTCTCTGT CAATGAGAAG
    GAGTTCAACC AATACGCTGA GGGGAAGCTC TCCGTCTTTG CTGAAACCTA CGAGAACCAG
    ACCAAGCTGG CGCTGGTGGG GAACTGGGGC ACCACCGGCC TGACTTACCC CAAATACTCC
    GATGTCACCG GCAAGATCAA GCTCCCCAAG GACAGCTTCC GGCCCTCCAC GGGCTGGACC
    TGGGCTGGTG ATTGGTTCAT CTGCCCAGAG AAGACGATGC TGCACGAGGC AGACGCGGGG
    CACCTGAGCT TCGTGGAGGA GGTGTTTGAG AACCAGGTGC GGCTGCCGGG CGGGCAGTGG
    ATCCACATGT CCGATGCCTA CACCGATGTG AATGGGGAGA AGGTTCTGCC CAAGGATGAG
    ATCGAGTGTC CTCCGGGCTG GAAATGGGAA GACGTGGAGT GGGACACTGA CCTCAACAGA
    GCCGTGGATG AGAAAGGCTG GGAGTACGGC ATCACCATCC CTCCGGACCG CCGGCCCAAG
    GCCTGGGTGC CAGCTGAGAA GATGTACCAC ACCAACCGGC GCCGGCGCTG GGTGCGACTG
    CGCAGCAGGG ACCTGGCCCA GATGGAGACT GTCAGGAAGC ACAAGCAGGA CGAGCAGGAC
    GGCGAGGGCT GGGAATACGC CTCGCTGCTG GGCTGGCGCT TCCACCTGCG GCCGCGTCGC
    ACCGATGCCT TCCGCCGCCG CCGTTGGAGA CGTAGGATGG AGCCCTTGGA GCGCACCGGG
    GCTGCTGCTG TGTTCGCCTT GGAGGGGGCC CTGGGAGGAA TGACGGACGA CAAAAGCGAC
    GATGGCAAAT CCGTCTCCAC GCTGAGTTTC GGCGTCAACA GACCCACCAT CTCCTGCATC
    TTCGACTATG GGAACCGCTA CCACCTGCGC TGCTACATGT ACCAGGCACG GGACCTGGCT
    GCCATGGACA AGGACAGCTT CTCGGACCCC TATGCCATCG TTTCCTTCCT CCACCAAAGC
    CAGAAGACGG TGGTGATCAA GAACACCCTG AACCCCACGT GGGACCAGAC GCTCATCTTC
    TACGAGATAG AGATCTTCGG AGACCCCAAG AACGTGTCTG ACTGCCCTCC CAGCATCGTG
    GTGGAGATAT ACGACCACGA CACCTACGGC GCTGATGAGT TCATGGGGCG CTGCGTGTGC
    CGGCCCAGCC TGCAGCGCGC GCCGCGCCTC TCCTGGCACC CCGTGCTCAA AGGCGGCAGG
    AACGTCGGCG AGCTGCTGGC TGCCTTCGAG CTCATCCAGA GGGAGAAGCC GGCTGTCCAC
    CACATCCCTG GCTTCGAGAA CGAGCTGTCC TCCGCTCTGG ATGAGTCTGA GGACTCTGAC
    CTGCCCTACC CACCGCCCCA ACGGGAACCC AACATCTTCA TGGTCCCCCA AGGGATCAAA
    CCGGTCCTGC AGCGCACGGC CATCGAGATC CTGGCCTGGG GGCTGAGGAA CCTCAAGAGC
    TACCAGCTGG CCAGCGTCAC CTCACCCAGC CTGCTGGTGG AGTGCGGGGG GCAGCTCGTG
    CAGTCCTGTG TCATCAAGAA CGTCAAGAAA AACCCCAACT TTGATGTCTG TGTGCTCTTC
    ATGGAAGTGC GTCTGCCCAG CGAGAGCCTC TACAGTCCCC CCATCATCAT CAAAATCATC
    GACAACCGTC CGTTCGGCCG CAGGCCCGTG GTGGGGCAGT GCACCATCCG CTCACTGGAG
    GAATTCTACT GTGACCCCTA TGGTGAGGAG ACGGATGGTG CCCAGGAGCA GGCAGATGAT
    GTGAGCCTCA CGCCCAGGGA TGATGTCCTC ATTGACATTG ATGACAAAGA GCCTCTCCTT
    CCCACCCAGG AGGAAGAATT CATTGACTGG TGGAGCAAAT TCTACGCTTC CACAGGAGAG
    AGGGAGAAGT GTGGCTCCTA CTTGGAGAAG GGCTTTGACA CCCTGCAGGT CTATGAGACG
    GAGCTGGAGC GCGTGGAGGC CTTCGAGCAG CTCTCTGACT TCTGCCACAC CTTCAGGCTG
    TACCGTGGCC GGGTGCAGGA TGCCAACGAT GATCCCTCAG TTGTGGGCGA GTTCAAGGGC
    TCCTTCAAAA TCTACCCTCT GCCCGATGAC CCACGCGTGC CGCTGCCGCC GCGGCAGTTC
    CACCAACTGC CAGCCAGGGG TCCCCAGGAG TGTCTCGTCA GGGTCTACAT CATCCGTGCC
    TTCGGTCTGC AGCCCAAGGA TGCCAATGGG AAGTGCGATC CGTATGTGAA GATTTCAGTG
    GGAAAGAAAT CCATAAACGA CCAAGAAAAT TACCTCCCCT CTACGCTGGA GCCTGTCTTT
    GGAAAGATGT TTGAGTTGAG CTGCACTCTG CCCCTGGAGA AGGACCTGAA GGTCACACTC
    TATGACTACG ACCTCCTGTC CAAGGATGAG AAGATTGGGG AGACCGTCAT CGACCTGGAG
    AATCGGTTCC TGTCGAAATA TGGGGCGCGC TGTGGGCTTC CCCAGACCTA CTGCATCTCT
    GGGCCCAACC AGTGGCGAGA CCAGCTCCGT CCCTCCCAAC TGCTCCACCT CTTCTCCCTG
    CAGCACCACT ACAAGGCTCC CACCTACACG TCCGACCGGC TCATCTTCCG GGAGCAGGAG
    TACATCCTCT CCGAGCTGGA GGATGGGAAA CCCCCCAACC CTCACCTGGG GCCGGCAGAG
    GAGCGGCTGG CCCTGCATGC ACTGCGGAAA CAAGGGCTGG TGCCGGAGCA CGTGGAGACC
    CGGCCCCTCT ACAGCCCCCT GCAGCCAGAG ATCGAGCAGG GAAAGCTACA GATGTGGGTG
    GACCTGTTTC CGAAATCCTT GGGTCAGCCT GGCCCCCCTT TCAACATCAC ACCACGCAAA
    GCCAAGAGGT TCTACTTGCG CTGCATCATC TGGAACACCA GGGACGTCAT CCTCGATGAC
    CTCAGCATCA CTGGGGAGAA GATGAGTGAC ATCTATGTCA AAGGCTGGCT GGTTGGCCAC
    GAGGAGAACA AGCAGAAGAC AGACGTGCAC TACAGGTCGA TGGGAGGAGA AGGCAACTTC
    AACTGGAGGT TCATCTTCCC CTTCGACTAT CTCCCTGCCG AGCAGATGTG TTGCGTTGCA
    AAGAAGGAGC ATTTCTGGAG CTTGGATAAG ACGGAAAACA AGATTTCACC GCAGCTGATC
    TTCCAGATCT GGGACAATGA CAAGTTCTCC TTCGATGACT ATTTGGGCTC CATCCAGATG
    GATCTCAACA GGATGCCCAA GCCTGCCAAG ACTGCTGAGA AGTGCTCCTT AGAGCTCATA
    GATGACAGCT TGTCTGCAAG CCGCTTTGTG TCCCTCTTTG AGCAGAAGAC TGTCAAGGGC
    TGGTGGCCCT GCATTGCAGA GCAGGATGAG AAGAAGATCC TGGCAGGAAA ACTGGAAATG
    ACTTTGGAAA TCGTGGCTGA ACACGAGCAT GAAGAGCGGC CAGCTGGCGT GGGTCGGGAT
    GAGCCAAACA TGAACCCCAA GCTGGAGGAC CCCAAGCGCC CAGAGACCTC CTTCCTGTGG
    TTCACCTCCC CGTACAAGAC CCTGAAGTAC ATCCTGTGGC GCCGGTACAA GTGGCTGCTC
    ATCCTGGCCA TCGTGCTCTT TGTTTTGCTG CTCTTCCTTG GGATCTTCAT CTACGCCTTC
    CCGAACTACG CTGCTATGAA GCTGGTGAAA CCTTTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150249
    Clone ID Related Accession (Same CDS sequence) XM_025149877.1
    Accession Version XM_025149877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product dysferlin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGCT GCGTGCTGCT GCGCGCCCAA CTGCGCCGCC CCGATAAGGG GGACCGGCAG 
    AGCGACCCCG TGGCCAGCCT GACCTTCCGA GGGGTGAAGA AGAAGACGAA GGTCATCAAG
    AACAGCATCA ACCCCGTATG GAACGAGGGC TTTGAATGGG ATCTGAAGGG CGTTGCTTTG
    GACCTGGGAT CTGAGCTCTG TGTTGTGGTT AAGGACCATG AGACTGTGGG GAGGAATCGG
    TTTTTGGGAG AGGCCCGCGT CCCCCTACGG GATGTGCTGT CCTCCCCCAG CCTGGCGGCC
    TCCTATGATG TCCCATTGCT GGATAGCAAG AAGCAGAGCA CCGGGGCTTC CCTCATCCTA
    CAAGTGTCCT ACAGCCCTCC TCCGGGCGCT GCCCCCCTCT TCCCTCCCCC AACCCCCCCC
    GAGGCCGCCC CGGCGGCGGT GGCAGAGTCC GACACTCTCA CTGAAGTGGC CACCGAGGAG
    GAGGGGGAGG ACCAGGCGGT GACGGGGGAC GAGACGGAGC CCCCATCCTC GGGGCCGCCG
    GGCTCGGAGC CGCCCTCGGT GCCCCACAGA CCCCCGGCTC CCCGCGGCCG TGGGGTGAAG
    CGCAGGAGGA GCTCGCCCAA AAAGCCGCTG TCCAACAAAC CTCAGGACTT CCAGATCCGG
    GTTCGGGTGA TCGAGGCCCG GCAGCTGCCC GGCGTTAACA TCCGGCCCGT GGTGAAGGTG
    ACGGCGGCGG GGCAGACCAA GCGGACCAGG ATACGCAAAG GCAACAGCCC CCTCTTCGAC
    GAGACCTTCT TCTTCAACGT CTTCGAGTCG CCAGCCGAGC TGTTCGACGT GCCCGTCTTC
    ATCACGGTCC TGGACTCGCG CTCCTTCCGC ATGGATGCGG TGATCGGAGA GTTTCGGATG
    GACGTTGAGA GCATCTATTC GGAGCCGAAA CACGCGATCC TCAGGCGGTG GCTGCTGCTC
    TCTGACCCGG AGGACTTCAC GGCGGGGGCA AAGGGGTACC TGAAGGTCAG CCTGCTTGTG
    CTGGGCCCCG GGGACGAAGC TCCTGTGGAG AAGAAGGACG TCTCTGAAGA CAAGGAAGAC
    ATTGAGGGGA ACCTCCTGCG ACCCACGGGG GTCACCTTGC GAGGGGCTCA GTTCTGCCTG
    AAGATCTTCA AGGCTGAGGA TTTGCCCCAG ATGGACGACG CCGTCATGGA CAACGTCCGG
    CAGATCTTCG GCTTCGAAAG CAACAAGAAG AACCTGGTTG ACCCCTTTGT GGAAGTCAGC
    TTTGCGGGGA AGACGCTCTA CTCGAGGATC CTGGAGAAGA ATGCCAACCC GCAGTGGAAT
    CAGTGCCTGA TGCTGCCGGT GATGTTCCCT TCCATGTGCG AGAAGATGAG GATCCGGGTG
    ACCGACTGGG ACCGTCTCAC ACACAACGAC GTCGTCGGCA CCGCCTACCT CTGCATGTCC
    AAGATCTCTG CGCCCGGCGG GGAGCTGGAG GTGGACGACG GGCTGGGCTT CCTGCCGACC
    TTCGGCCCCT GCTACGTCAA CCTGTACGGC AGCCCCCGTG AGTTCACCGG CTTCCCGGAT
    CCGTACGAGG CGCTCAATTT AGGAAAGGGC GAGGGCGTCG CGTACCGCGG CCGGGTGCTG
    CTGGAGCTGG AGACCAAGCT GGCGGAGCAC GGGGAGCTGA AGGTGGAGGA CATCTGTGCG
    GATGACATCC TGCGGGTGGA GAAGTACCTG CGGCGGAGGA AGTACTCCCT CTTTGCTGCC
    TTCTACTCGG CCACCATGCT GCAGGACGTG GACGATGCCA TCCAGTTTGA GGTCAGCATC
    GGCAACTACG GCAACAAGTT CGACAACACC TGCTTGCCCT TGGCCTCCAC CACGCAGTAC
    AGCCGGGCCG TGTTCGATGG TTGCCAGTAT TACTACCTGC CCTGGGGCAA CGTGAAGCCC
    GTGGTGGTGC TGTCCTCCTA TTGGGAAGAT ATCAGCTACC GAACCGACAC TCAGAACCTC
    CTCCAGCACG CAGCAGACAG GCTGGAAGCC AACCTGGAGC GGGTCCACCT CGCGCTCAAG
    GCCAACGGCG CGCCGGGCGA GCTGGAGTCC CTGGCTGCCC AGCTGATGGA CGACGTGATT
    GCAGACTGCA GTGTTCCGCT GCCCGACGTG CTGGGGAAGG CGGCCAGCAC ACACCTGGAC
    CAGAACCTGT ACCGCTTCCG CAGCACCAAC CTGGAGCAGA TGGTGCGGGC GGCCCTGAGG
    CTGAAGCACG AGGAGCTGAG CCTGCCGGCC GTGCTGGAGC AGGCGGAGGA CTGGCTGTGC
    CGGCTGCGTG CCATGGCTGA GGAGCCCCAG AACAGCCTCC CAGACGTGGT GATCTGGATG
    CTGCGGGGTG AGAAGCGCGT TGCCTACGCC CGCGTGCCGG CCCGCGAGGT GCTCTACTCC
    CAAAGTGGCC CCAACTGCTG CGGTAGGAAC TGTGGCAAGC TGCAGACCAT CTTCCTGAAG
    TACCCCCAGG AGGAGGCGAT GGGGCCGCGC ATCCCAGCCC AGATCCGCGT CCAGCTCTGG
    TTTGGGCTCT CTGTCAATGA GAAGGAGTTC AACCAATACG CTGAGGGGAA GCTCTCCGTC
    TTTGCTGAAA CCTACGAGAA CCAGACCAAG CTGGCGCTGG TGGGGAACTG GGGCACCACC
    GGCCTGACTT ACCCCAAATA CTCCGATGTC ACCGGCAAGA TCAAGCTCCC CAAGGACAGC
    TTCCGGCCCT CCACGGGCTG GACCTGGGCT GGTGATTGGT TCATCTGCCC AGAGAAGACG
    ATGCTGCACG AGGCAGACGC GGGGCACCTG AGCTTCGTGG AGGAGGTGTT TGAGAACCAG
    GTGCGGCTGC CGGGCGGGCA GTGGATCCAC ATGTCCGATG CCTACACCGA TGTGAATGGG
    GAGAAGGTTC TGCCCAAGGA TGAGATCGAG TGTCCTCCGG GCTGGAAATG GGAAGACGTG
    GAGTGGGACA CTGACCTCAA CAGAGCCGTG GATGAGAAAG GCTGGGAGTA CGGCATCACC
    ATCCCTCCGG ACCGCCGGCC CAAGGCCTGG GTGCCAGCTG AGAAGATGTA CCACACCAAC
    CGGCGCCGGC GCTGGGTGCG ACTGCGCAGC AGGGACCTGG CCCAGATGGA GACTGTCAGG
    AAGCACAAGC AGGACGAGCA GGACGGCGAG GGCTGGGAAT ACGCCTCGCT GCTGGGCTGG
    CGCTTCCACC TGCGGCCGCG TCGCACCGAT GCCTTCCGCC GCCGCCGTTG GAGACGTAGG
    ATGGAGCCCT TGGAGCGCAC CGGGGCTGCT GCTGTGTTCG CCTTGGAGGG GGCCCTGGGA
    GGAATGACGG ACGACAAAAG CGACGATGGC AAATCCGTCT CCACGCTGAG TTTCGGCGTC
    AACAGACCCA CCATCTCCTG CATCTTCGAC TATGGGAACC GCTACCACCT GCGCTGCTAC
    ATGTACCAGG CACGGGACCT GGCTGCCATG GACAAGGACA GCTTCTCGGA CCCCTATGCC
    ATCGTTTCCT TCCTCCACCA AAGCCAGAAG ACGGTGGTGA TCAAGAACAC CCTGAACCCC
    ACGTGGGACC AGACGCTCAT CTTCTACGAG ATAGAGATCT TCGGAGACCC CAAGAACGTG
    TCTGACTGCC CTCCCAGCAT CGTGGTGGAG ATATACGACC ACGACACCTA CGGCGCTGAT
    GAGTTCATGG GGCGCTGCGT GTGCCGGCCC AGCCTGCAGC GCGCGCCGCG CCTCTCCTGG
    CACCCCGTGC TCAAAGGCGG CAGGAACGTC GGCGAGCTGC TGGCTGCCTT CGAGCTCATC
    CAGAGGGAGA AGCCGGCTGT CCACCACATC CCTGGCTTCG AGAACGAGCT GTCCTCCGCT
    CTGGATGAGT CTGAGGACTC TGACCTGCCC TACCCACCGC CCCAACGGGA ACCCAACATC
    TTCATGGTCC CCCAAGGGAT CAAACCGGTC CTGCAGCGCA CGGCCATCGA GATCCTGGCC
    TGGGGGCTGA GGAACCTCAA GAGCTACCAG CTGGCCAGCG TCACCTCACC CAGCCTGCTG
    GTGGAGTGCG GGGGGCAGCT CGTGCAGTCC TGTGTCATCA AGAACGTCAA GAAAAACCCC
    AACTTTGATG TCTGTGTGCT CTTCATGGAA GTGCGTCTGC CCAGCGAGAG CCTCTACAGT
    CCCCCCATCA TCATCAAAAT CATCGACAAC CGTCCGTTCG GCCGCAGGCC CGTGGTGGGG
    CAGTGCACCA TCCGCTCACT GGAGGAATTC TACTGTGACC CCTATGGTGA GGAGACGGAT
    GGTGCCCAGG AGCAGGCAGA TGATGTGAGC CTCACGCCCA GGGATGATGT CCTCATTGAC
    ATTGATGACA AAGAGCCTCT CCTTCCCACC CAGGAGGAAG AATTCATTGA CTGGTGGAGC
    AAATTCTACG CTTCCACAGG AGAGAGGGAG AAGTGTGGCT CCTACTTGGA GAAGGGCTTT
    GACACCCTGC AGGTCTATGA GACGGAGCTG GAGCGCGTGG AGGCCTTCGA GCAGCTCTCT
    GACTTCTGCC ACACCTTCAG GCTGTACCGT GGCCGGGTGC AGGATGCCAA CGATGATCCC
    TCAGTTGTGG GCGAGTTCAA GGGCTCCTTC AAAATCTACC CTCTGCCCGA TGACCCACGC
    GTGCCGCTGC CGCCGCGGCA GTTCCACCAA CTGCCAGCCA GGGGTCCCCA GGAGTGTCTC
    GTCAGGGTCT ACATCATCCG TGCCTTCGGT CTGCAGCCCA AGGATGCCAA TGGGAAGTGC
    GATCCGTATG TGAAGATTTC AGTGGGAAAG AAATCCATAA ACGACCAAGA AAATTACCTC
    CCCTCTACGC TGGAGCCTGT CTTTGGAAAG ATGTTTGAGT TGAGCTGCAC TCTGCCCCTG
    GAGAAGGACC TGAAGGTCAC ACTCTATGAC TACGACCTCC TGTCCAAGGA TGAGAAGATT
    GGGGAGACCG TCATCGACCT GGAGAATCGG TTCCTGTCGA AATATGGGGC GCGCTGTGGG
    CTTCCCCAGA CCTACTGCAT CTCTGGGCCC AACCAGTGGC GAGACCAGCT CCGTCCCTCC
    CAACTGCTCC ACCTCTTCTC CCTGCAGCAC CACTACAAGG CTCCCACCTA CACGTCCGAC
    CGGCTCATCT TCCGGGAGCA GGAGTACATC CTCTCCGAGC TGGAGGATGG GAAACCCCCC
    AACCCTCACC TGGGGCCGGC AGAGGAGCGG CTGGCCCTGC ATGCACTGCG GAAACAAGGG
    CTGGTGCCGG AGCACGTGGA GACCCGGCCC CTCTACAGCC CCCTGCAGCC AGAGATCGAG
    CAGGGAAAGC TACAGATGTG GGTGGACCTG TTTCCGAAAT CCTTGGGTCA GCCTGGCCCC
    CCTTTCAACA TCACACCACG CAAAGCCAAG AGGTTCTACT TGCGCTGCAT CATCTGGAAC
    ACCAGGGACG TCATCCTCGA TGACCTCAGC ATCACTGGGG AGAAGATGAG TGACATCTAT
    GTCAAAGGCT GGCTGGTTGG CCACGAGGAG AACAAGCAGA AGACAGACGT GCACTACAGG
    TCGATGGGAG GAGAAGGCAA CTTCAACTGG AGGTTCATCT TCCCCTTCGA CTATCTCCCT
    GCCGAGCAGA TGTGTTGCGT TGCAAAGAAG GAGCATTTCT GGAGCTTGGA TAAGACGGAA
    AACAAGATTT CACCGCAGCT GATCTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTCGAT
    GACTATTTGG GCTCCATCCA GATGGATCTC AACAGGATGC CCAAGCCTGC CAAGACTGCT
    GAGAAGTGCT CCTTAGAGCT CATAGATGAC AGCTTGTCTG CAAGCCGCTT TGTGTCCCTC
    TTTGAGCAGA AGACTGTCAA GGGCTGGTGG CCCTGCATTG CAGAGCAGGA TGAGAAGAAG
    ATCCTGGCAG GAAAACTGGA AATGACTTTG GAAATCGTGG CTGAACACGA GCATGAAGAG
    CGGCCAGCTG GCGTGGGTCG GGATGAGCCA AACATGAACC CCAAGCTGGA GGACCCCAAG
    CGCCCAGAGA CCTCCTTCCT GTGGTTCACC TCCCCGTACA AGACCCTGAA GTACATCCTG
    TGGCGCCGGT ACAAGTGGCT GCTCATCCTG GCCATCGTGC TCTTTGTTTT GCTGCTCTTC
    CTTGGGATCT TCATCTACGC CTTCCCGAAC TACGCTGCTA TGAAGCTGGT GAAACCTTTC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005645.1
    CDS36..6221
    Translation

    Target ORF information:

    RefSeq Version XM_025149877.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGCT GCGTGCTGCT GCGCGCCCAA CTGCGCCGCC CCGATAAGGG GGACCGGCAG 
    AGCGACCCCG TGGCCAGCCT GACCTTCCGA GGGGTGAAGA AGAAGACGAA GGTCATCAAG
    AACAGCATCA ACCCCGTATG GAACGAGGGC TTTGAATGGG ATCTGAAGGG CGTTGCTTTG
    GACCTGGGAT CTGAGCTCTG TGTTGTGGTT AAGGACCATG AGACTGTGGG GAGGAATCGG
    TTTTTGGGAG AGGCCCGCGT CCCCCTACGG GATGTGCTGT CCTCCCCCAG CCTGGCGGCC
    TCCTATGATG TCCCATTGCT GGATAGCAAG AAGCAGAGCA CCGGGGCTTC CCTCATCCTA
    CAAGTGTCCT ACAGCCCTCC TCCGGGCGCT GCCCCCCTCT TCCCTCCCCC AACCCCCCCC
    GAGGCCGCCC CGGCGGCGGT GGCAGAGTCC GACACTCTCA CTGAAGTGGC CACCGAGGAG
    GAGGGGGAGG ACCAGGCGGT GACGGGGGAC GAGACGGAGC CCCCATCCTC GGGGCCGCCG
    GGCTCGGAGC CGCCCTCGGT GCCCCACAGA CCCCCGGCTC CCCGCGGCCG TGGGGTGAAG
    CGCAGGAGGA GCTCGCCCAA AAAGCCGCTG TCCAACAAAC CTCAGGACTT CCAGATCCGG
    GTTCGGGTGA TCGAGGCCCG GCAGCTGCCC GGCGTTAACA TCCGGCCCGT GGTGAAGGTG
    ACGGCGGCGG GGCAGACCAA GCGGACCAGG ATACGCAAAG GCAACAGCCC CCTCTTCGAC
    GAGACCTTCT TCTTCAACGT CTTCGAGTCG CCAGCCGAGC TGTTCGACGT GCCCGTCTTC
    ATCACGGTCC TGGACTCGCG CTCCTTCCGC ATGGATGCGG TGATCGGAGA GTTTCGGATG
    GACGTTGAGA GCATCTATTC GGAGCCGAAA CACGCGATCC TCAGGCGGTG GCTGCTGCTC
    TCTGACCCGG AGGACTTCAC GGCGGGGGCA AAGGGGTACC TGAAGGTCAG CCTGCTTGTG
    CTGGGCCCCG GGGACGAAGC TCCTGTGGAG AAGAAGGACG TCTCTGAAGA CAAGGAAGAC
    ATTGAGGGGA ACCTCCTGCG ACCCACGGGG GTCACCTTGC GAGGGGCTCA GTTCTGCCTG
    AAGATCTTCA AGGCTGAGGA TTTGCCCCAG ATGGACGACG CCGTCATGGA CAACGTCCGG
    CAGATCTTCG GCTTCGAAAG CAACAAGAAG AACCTGGTTG ACCCCTTTGT GGAAGTCAGC
    TTTGCGGGGA AGACGCTCTA CTCGAGGATC CTGGAGAAGA ATGCCAACCC GCAGTGGAAT
    CAGTGCCTGA TGCTGCCGGT GATGTTCCCT TCCATGTGCG AGAAGATGAG GATCCGGGTG
    ACCGACTGGG ACCGTCTCAC ACACAACGAC GTCGTCGGCA CCGCCTACCT CTGCATGTCC
    AAGATCTCTG CGCCCGGCGG GGAGCTGGAG GTGGACGACG GGCTGGGCTT CCTGCCGACC
    TTCGGCCCCT GCTACGTCAA CCTGTACGGC AGCCCCCGTG AGTTCACCGG CTTCCCGGAT
    CCGTACGAGG CGCTCAATTT AGGAAAGGGC GAGGGCGTCG CGTACCGCGG CCGGGTGCTG
    CTGGAGCTGG AGACCAAGCT GGCGGAGCAC GGGGAGCTGA AGGTGGAGGA CATCTGTGCG
    GATGACATCC TGCGGGTGGA GAAGTACCTG CGGCGGAGGA AGTACTCCCT CTTTGCTGCC
    TTCTACTCGG CCACCATGCT GCAGGACGTG GACGATGCCA TCCAGTTTGA GGTCAGCATC
    GGCAACTACG GCAACAAGTT CGACAACACC TGCTTGCCCT TGGCCTCCAC CACGCAGTAC
    AGCCGGGCCG TGTTCGATGG TTGCCAGTAT TACTACCTGC CCTGGGGCAA CGTGAAGCCC
    GTGGTGGTGC TGTCCTCCTA TTGGGAAGAT ATCAGCTACC GAACCGACAC TCAGAACCTC
    CTCCAGCACG CAGCAGACAG GCTGGAAGCC AACCTGGAGC GGGTCCACCT CGCGCTCAAG
    GCCAACGGCG CGCCGGGCGA GCTGGAGTCC CTGGCTGCCC AGCTGATGGA CGACGTGATT
    GCAGACTGCA GTGTTCCGCT GCCCGACGTG CTGGGGAAGG CGGCCAGCAC ACACCTGGAC
    CAGAACCTGT ACCGCTTCCG CAGCACCAAC CTGGAGCAGA TGGTGCGGGC GGCCCTGAGG
    CTGAAGCACG AGGAGCTGAG CCTGCCGGCC GTGCTGGAGC AGGCGGAGGA CTGGCTGTGC
    CGGCTGCGTG CCATGGCTGA GGAGCCCCAG AACAGCCTCC CAGACGTGGT GATCTGGATG
    CTGCGGGGTG AGAAGCGCGT TGCCTACGCC CGCGTGCCGG CCCGCGAGGT GCTCTACTCC
    CAAAGTGGCC CCAACTGCTG CGGTAGGAAC TGTGGCAAGC TGCAGACCAT CTTCCTGAAG
    TACCCCCAGG AGGAGGCGAT GGGGCCGCGC ATCCCAGCCC AGATCCGCGT CCAGCTCTGG
    TTTGGGCTCT CTGTCAATGA GAAGGAGTTC AACCAATACG CTGAGGGGAA GCTCTCCGTC
    TTTGCTGAAA CCTACGAGAA CCAGACCAAG CTGGCGCTGG TGGGGAACTG GGGCACCACC
    GGCCTGACTT ACCCCAAATA CTCCGATGTC ACCGGCAAGA TCAAGCTCCC CAAGGACAGC
    TTCCGGCCCT CCACGGGCTG GACCTGGGCT GGTGATTGGT TCATCTGCCC AGAGAAGACG
    ATGCTGCACG AGGCAGACGC GGGGCACCTG AGCTTCGTGG AGGAGGTGTT TGAGAACCAG
    GTGCGGCTGC CGGGCGGGCA GTGGATCCAC ATGTCCGATG CCTACACCGA TGTGAATGGG
    GAGAAGGTTC TGCCCAAGGA TGAGATCGAG TGTCCTCCGG GCTGGAAATG GGAAGACGTG
    GAGTGGGACA CTGACCTCAA CAGAGCCGTG GATGAGAAAG GCTGGGAGTA CGGCATCACC
    ATCCCTCCGG ACCGCCGGCC CAAGGCCTGG GTGCCAGCTG AGAAGATGTA CCACACCAAC
    CGGCGCCGGC GCTGGGTGCG ACTGCGCAGC AGGGACCTGG CCCAGATGGA GACTGTCAGG
    AAGCACAAGC AGGACGAGCA GGACGGCGAG GGCTGGGAAT ACGCCTCGCT GCTGGGCTGG
    CGCTTCCACC TGCGGCCGCG TCGCACCGAT GCCTTCCGCC GCCGCCGTTG GAGACGTAGG
    ATGGAGCCCT TGGAGCGCAC CGGGGCTGCT GCTGTGTTCG CCTTGGAGGG GGCCCTGGGA
    GGAATGACGG ACGACAAAAG CGACGATGGC AAATCCGTCT CCACGCTGAG TTTCGGCGTC
    AACAGACCCA CCATCTCCTG CATCTTCGAC TATGGGAACC GCTACCACCT GCGCTGCTAC
    ATGTACCAGG CACGGGACCT GGCTGCCATG GACAAGGACA GCTTCTCGGA CCCCTATGCC
    ATCGTTTCCT TCCTCCACCA AAGCCAGAAG ACGGTGGTGA TCAAGAACAC CCTGAACCCC
    ACGTGGGACC AGACGCTCAT CTTCTACGAG ATAGAGATCT TCGGAGACCC CAAGAACGTG
    TCTGACTGCC CTCCCAGCAT CGTGGTGGAG ATATACGACC ACGACACCTA CGGCGCTGAT
    GAGTTCATGG GGCGCTGCGT GTGCCGGCCC AGCCTGCAGC GCGCGCCGCG CCTCTCCTGG
    CACCCCGTGC TCAAAGGCGG CAGGAACGTC GGCGAGCTGC TGGCTGCCTT CGAGCTCATC
    CAGAGGGAGA AGCCGGCTGT CCACCACATC CCTGGCTTCG AGAACGAGCT GTCCTCCGCT
    CTGGATGAGT CTGAGGACTC TGACCTGCCC TACCCACCGC CCCAACGGGA ACCCAACATC
    TTCATGGTCC CCCAAGGGAT CAAACCGGTC CTGCAGCGCA CGGCCATCGA GATCCTGGCC
    TGGGGGCTGA GGAACCTCAA GAGCTACCAG CTGGCCAGCG TCACCTCACC CAGCCTGCTG
    GTGGAGTGCG GGGGGCAGCT CGTGCAGTCC TGTGTCATCA AGAACGTCAA GAAAAACCCC
    AACTTTGATG TCTGTGTGCT CTTCATGGAA GTGCGTCTGC CCAGCGAGAG CCTCTACAGT
    CCCCCCATCA TCATCAAAAT CATCGACAAC CGTCCGTTCG GCCGCAGGCC CGTGGTGGGG
    CAGTGCACCA TCCGCTCACT GGAGGAATTC TACTGTGACC CCTATGGTGA GGAGACGGAT
    GGTGCCCAGG AGCAGGCAGA TGATGTGAGC CTCACGCCCA GGGATGATGT CCTCATTGAC
    ATTGATGACA AAGAGCCTCT CCTTCCCACC CAGGAGGAAG AATTCATTGA CTGGTGGAGC
    AAATTCTACG CTTCCACAGG AGAGAGGGAG AAGTGTGGCT CCTACTTGGA GAAGGGCTTT
    GACACCCTGC AGGTCTATGA GACGGAGCTG GAGCGCGTGG AGGCCTTCGA GCAGCTCTCT
    GACTTCTGCC ACACCTTCAG GCTGTACCGT GGCCGGGTGC AGGATGCCAA CGATGATCCC
    TCAGTTGTGG GCGAGTTCAA GGGCTCCTTC AAAATCTACC CTCTGCCCGA TGACCCACGC
    GTGCCGCTGC CGCCGCGGCA GTTCCACCAA CTGCCAGCCA GGGGTCCCCA GGAGTGTCTC
    GTCAGGGTCT ACATCATCCG TGCCTTCGGT CTGCAGCCCA AGGATGCCAA TGGGAAGTGC
    GATCCGTATG TGAAGATTTC AGTGGGAAAG AAATCCATAA ACGACCAAGA AAATTACCTC
    CCCTCTACGC TGGAGCCTGT CTTTGGAAAG ATGTTTGAGT TGAGCTGCAC TCTGCCCCTG
    GAGAAGGACC TGAAGGTCAC ACTCTATGAC TACGACCTCC TGTCCAAGGA TGAGAAGATT
    GGGGAGACCG TCATCGACCT GGAGAATCGG TTCCTGTCGA AATATGGGGC GCGCTGTGGG
    CTTCCCCAGA CCTACTGCAT CTCTGGGCCC AACCAGTGGC GAGACCAGCT CCGTCCCTCC
    CAACTGCTCC ACCTCTTCTC CCTGCAGCAC CACTACAAGG CTCCCACCTA CACGTCCGAC
    CGGCTCATCT TCCGGGAGCA GGAGTACATC CTCTCCGAGC TGGAGGATGG GAAACCCCCC
    AACCCTCACC TGGGGCCGGC AGAGGAGCGG CTGGCCCTGC ATGCACTGCG GAAACAAGGG
    CTGGTGCCGG AGCACGTGGA GACCCGGCCC CTCTACAGCC CCCTGCAGCC AGAGATCGAG
    CAGGGAAAGC TACAGATGTG GGTGGACCTG TTTCCGAAAT CCTTGGGTCA GCCTGGCCCC
    CCTTTCAACA TCACACCACG CAAAGCCAAG AGGTTCTACT TGCGCTGCAT CATCTGGAAC
    ACCAGGGACG TCATCCTCGA TGACCTCAGC ATCACTGGGG AGAAGATGAG TGACATCTAT
    GTCAAAGGCT GGCTGGTTGG CCACGAGGAG AACAAGCAGA AGACAGACGT GCACTACAGG
    TCGATGGGAG GAGAAGGCAA CTTCAACTGG AGGTTCATCT TCCCCTTCGA CTATCTCCCT
    GCCGAGCAGA TGTGTTGCGT TGCAAAGAAG GAGCATTTCT GGAGCTTGGA TAAGACGGAA
    AACAAGATTT CACCGCAGCT GATCTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTCGAT
    GACTATTTGG GCTCCATCCA GATGGATCTC AACAGGATGC CCAAGCCTGC CAAGACTGCT
    GAGAAGTGCT CCTTAGAGCT CATAGATGAC AGCTTGTCTG CAAGCCGCTT TGTGTCCCTC
    TTTGAGCAGA AGACTGTCAA GGGCTGGTGG CCCTGCATTG CAGAGCAGGA TGAGAAGAAG
    ATCCTGGCAG GAAAACTGGA AATGACTTTG GAAATCGTGG CTGAACACGA GCATGAAGAG
    CGGCCAGCTG GCGTGGGTCG GGATGAGCCA AACATGAACC CCAAGCTGGA GGACCCCAAG
    CGCCCAGAGA CCTCCTTCCT GTGGTTCACC TCCCCGTACA AGACCCTGAA GTACATCCTG
    TGGCGCCGGT ACAAGTGGCT GCTCATCCTG GCCATCGTGC TCTTTGTTTT GCTGCTCTTC
    CTTGGGATCT TCATCTACGC CTTCCCGAAC TACGCTGCTA TGAAGCTGGT GAAACCTTTC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35945
    Clone ID Related Accession (Same CDS sequence) XM_015286070.1
    Accession Version XM_015286070.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product dysferlin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455866.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGCT GCGTGCTGCT GCGCGCCCAA CTGCGCCGCC CCGATAAGGG GGACCGGCAG 
    AGCGACCCCG TGGCCAGCCT GACCTTCCGA GGGGTGAAGA AGAAGACGAA GGTCATCAAG
    AACAGCATCA ACCCCGTATG GAACGAGGGC TTTGAATGGG ATCTGAAGGG CGTTGCTTTG
    GACCTGGGAT CTGAGCTCTG TGTTGTGGTT AAGGACCATG AGACTGTGGG GAGGAATCGG
    TTTTTGGGAG AGGCCCGCGT CCCCCTACGG GATGTGCTGT CCTCCCCCAG CCTGGCGGCC
    TCCTATGATG TCCCATTGCT GGATAGCAAG AAGCAGAGCA CCGGGGCTTC CCTCATCCTA
    CAAGTGTCCT ACAGCCCTCC TCCGGGCGCT GCCCCCCTCT TCCCTCCCCC AACCCCCCCG
    AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGCAACCTCC CTGCCTCCCG
    CCCCGCGCAG AAGTGGCCAC CGAGGAGGAG GGGGAGGACC AGGCGGTGAC GGGGGACGAG
    ACGGAGCCCC CATCCTCGGG GCCGCCGGGC TCGGAGCCGC CCTCGGTGCC CCACAGACCC
    CCGGCTCCCC GCGGCCGTGG GGTGAAGCGC AGGAGGAGCT CGCCCAAAAA GCCGCTGTCC
    AACAAACCTC AGGACTTCCA GATCCGGGTT CGGGTGATCG AGGCCCGGCA GCTGCCCGGC
    GTTAACATCC GGCCCGTGGT GAAGGTGACG GCGGCGGGGC AGACCAAGCG GACCAGGATA
    CGCAAAGGCA ACAGCCCCCT CTTCGACGAG ACCTTCTTCT TCAACGTCTT CGAGTCGCCA
    GCCGAGCTGT TCGACGTGCC CGTCTTCATC ACGGTCCTGG ACTCGCGCTC CTTCCGCATG
    GATGCGGTGA TCGGAGAGTT TCGGATGGAC GTTGAGAGCA TCTATTCGGA GCCGAAACAC
    GCGATCCTCA GGCGGTGGCT GCTGCTCTCT GACCCGGAGG ACTTCACGGC GGGGGCAAAG
    GGGTACCTGA AGGTCAGCCT GCTTGTGCTG GGCCCCGGGG ACGAAGCTCC TGTGGAGAAG
    AAGGACGTCT CTGAAGACAA GGAAGACATT GAGGGGAACC TCCTGCGACC CACGGGGGTC
    ACCTTGCGAG GGGCTCAGTT CTGCCTGAAG ATCTTCAAGG CTGAGGATTT GCCCCAGATG
    GACGACGCCG TCATGGACAA CGTCCGGCAG ATCTTCGGCT TCGAAAGCAA CAAGAAGAAC
    CTGGTTGACC CCTTTGTGGA AGTCAGCTTT GCGGGGAAGA CGCTCTACTC GAGGATCCTG
    GAGAAGAATG CCAACCCGCA GTGGAATCAG TGCCTGATGC TGCCGGTGAT GTTCCCTTCC
    ATGTGCGAGA AGATGAGGAT CCGGGTGACC GACTGGGACC GTCTCACACA CAACGACGTC
    GTCGGCACCG CCTACCTCTG CATGTCCAAG ATCTCTGCGC CCGGCGGGGA GCTGGAGGTG
    GACGACGGGC TGGGCTTCCT GCCGACCTTC GGCCCCTGCT ACGTCAACCT GTACGGCAGC
    CCCCGTGAGT TCACCGGCTT CCCGGATCCG TACGAGGCGC TCAATTTAGG AAAGGGCGAG
    GGCGTCGCGT ACCGCGGCCG GGTGCTGCTG GAGCTGGAGA CCAAGCTGGC GGAGCACGGG
    GAGCTGAAGG TGGAGGACAT CTGTGCGGAT GACATCCTGC GGGTGGAGAA GTACCTGCGG
    CGGAGGAAGT ACTCCCTCTT TGCTGCCTTC TACTCGGCCA CCATGCTGCA GGACGTGGAC
    GATGCCATCC AGTTTGAGGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CAACACCTGC
    TTGCCCTTGG CCTCCACCAC GCAGTACAGC CGGGCCGTGT TCGATGGTTG CCAGTATTAC
    TACCTGCCCT GGGGCAACGT GAAGCCCGTG GTGGTGCTGT CCTCCTATTG GGAAGATATC
    AGCTACCGAA CCGACACTCA GAACCTCCTC CAGCACGCAG CAGACAGGCT GGAAGCCAAC
    CTGGAGCGGG TCCACCTCGC GCTCAAGGCC AACGGCGCGC CGGGCGAGCT GGAGTCCCTG
    GCTGCCCAGC TGATGGACGA CGTGATTGCA GACTGCAGTG TTCCGCTGCC CGACGTGCTG
    GGGAAGGCGG CCAGCACACA CCTGGACCAG AACCTGTACC GCTTCCGCAG CACCAACCTG
    GAGCAGATGG TGCGGGCGGC CCTGAGGCTG AAGCACGAGG AGCTGAGCCT GCCGGCCGTG
    CTGGAGCAGG CGGAGGACTG GCTGTGCCGG CTGCGTGCCA TGGCTGAGGA GCCCCAGAAC
    AGCCTCCCAG ACGTGGTGAT CTGGATGCTG CGGGGTGAGA AGCGCGTTGC CTACGCCCGC
    GTGCCGGCCC GCGAGGTGCT CTACTCCCAA AGTGGCCCCA ACTGCTGCGG TAGGAACTGT
    GGCAAGCTGC AGACCATCTT CCTGAAGTAC CCCCAGGAGG AGGCGATGGG GCCGCGCATC
    CCAGCCCAGA TCCGCGTCCA GCTCTGGTTT GGGCTCTCTG TCAATGAGAA GGAGTTCAAC
    CAATACGCTG AGGGGAAGCT CTCCGTCTTT GCTGAAACCT ACGAGAACCA GACCAAGCTG
    GCGCTGGTGG GGAACTGGGG CACCACCGGC CTGACTTACC CCAAATACTC CGATGTCACC
    GGCAAGATCA AGCTCCCCAA GGACAGCTTC CGGCCCTCCA CGGGCTGGAC CTGGGCTGGT
    GATTGGTTCA TCTGCCCAGA GAAGACGATG CTGCACGAGG CAGACGCGGG GCACCTGAGC
    TTCGTGGAGG AGGTGTTTGA GAACCAGGTG CGGCTGCCGG GCGGGCAGTG GATCCACATG
    TCCGATGCCT ACACCGATGT GAATGGGGAG AAGGTTCTGC CCAAGGATGA GATCGAGTGT
    CCTCCGGGCT GGAAATGGGA AGACGTGGAG TGGGACACTG ACCTCAACAG AGCCGTGGAT
    GAGAAAGGCT GGGAGTACGG CATCACCATC CCTCCGGACC GCCGGCCCAA GGCCTGGGTG
    CCAGCTGAGA AGATGTACCA CACCAACCGG CGCCGGCGCT GGGTGCGACT GCGCAGCAGG
    GACCTGGCCC AGATGGAGAC TGTCAGGAAG CACAAGCAGG ACGAGCAGGA CGGCGAGGGC
    TGGGAATACG CCTCGCTGCT GGGCTGGCGC TTCCACCTGC GGCCGCGTCG CACCGATGCC
    TTCCGCCGCC GCCGTTGGAG ACGTAGGATG GAGCCCTTGG AGCGCACCGG GGCTGCTGCT
    GTGTTCGCCT TGGAGGGGGC CCTGGGAGGA ATGACGGACG ACAAAAGCGA CGATGGCAAA
    TCCGTCTCCA CGCTGAGTTT CGGCGTCAAC AGACCCACCA TCTCCTGCAT CTTCGACTAT
    GGGAACCGCT ACCACCTGCG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCCATGGAC
    AAGGACAGCT TCTCGGACCC CTATGCCATC GTTTCCTTCC TCCACCAAAG CCAGAAGACG
    GTGGTGATCA AGAACACCCT GAACCCCACG TGGGACCAGA CGCTCATCTT CTACGAGATA
    GAGATCTTCG GAGACCCCAA GAACGTGTCT GACTGCCCTC CCAGCATCGT GGTGGAGATA
    TACGACCACG ACACCTACGG CGCTGATGAG TTCATGGGGC GCTGCGTGTG CCGGCCCAGC
    CTGCAGCGCG CGCCGCGCCT CTCCTGGCAC CCCGTGCTCA AAGGCGGCAG GAACGTCGGC
    GAGCTGCTGG CTGCCTTCGA GCTCATCCAG AGGGAGAAGC CGGCTGTCCA CCACATCCCT
    GGCTTCGAGA ACGAGCTGTC CTCCGCTCTG GATGAGTCTG AGGACTCTGA CCTGCCCTAC
    CCACCGCCCC AACGGGAACC CAACATCTTC ATGGTCCCCC AAGGGATCAA ACCGGTCCTG
    CAGCGCACGG CCATCGAGAT CCTGGCCTGG GGGCTGAGGA ACCTCAAGAG CTACCAGCTG
    GCCAGCGTCA CCTCACCCAG CCTGCTGGTG GAGTGCGGGG GGCAGCTCGT GCAGTCCTGT
    GTCATCAAGA ACGTCAAGAA AAACCCCAAC TTTGATGTCT GTGTGCTCTT CATGGAAGTG
    CGTCTGCCCA GCGAGAGCCT CTACAGTCCC CCCATCATCA TCAAAATCAT CGACAACCGT
    CCGTTCGGCC GCAGGCCCGT GGTGGGGCAG TGCACCATCC GCTCACTGGA GGAATTCTAC
    TGTGACCCCT ATGGTGAGGA GACGGATGGT GCCCAGGAGC AGGCAGATGA TGTGAGCCTC
    ACGCCCAGGG ATGATGTCCT CATTGACATT GATGACAAAG AGCCTCTCCT TCCCACCCAG
    GAGGAAGAAT TCATTGACTG GTGGAGCAAA TTCTACGCTT CCACAGGAGA GAGGGAGAAG
    TGTGGCTCCT ACTTGGAGAA GGGCTTTGAC ACCCTGCAGG TCTATGAGAC GGAGCTGGAG
    CGCGTGGAGG CCTTCGAGCA GCTCTCTGAC TTCTGCCACA CCTTCAGGCT GTACCGTGGC
    CGGGTGCAGG ATGCCAACGA TGATCCCTCA GTTGTGGGCG AGTTCAAGGG CTCCTTCAAA
    ATCTACCCTC TGCCCGATGA CCCACGCGTG CCGCTGCCGC CGCGGCAGTT CCACCAACTG
    CCAGCCAGGG GTCCCCAGGA GTGTCTCGTC AGGGTCTACA TCATCCGTGC CTTCGGTCTG
    CAGCCCAAGG ATGCCAATGG GAAGTGCGAT CCGTATGTGA AGATTTCAGT GGGAAAGAAA
    TCCATAAACG ACCAAGAAAA TTACCTCCCC TCTACGCTGG AGCCTGTCTT TGGAAAGATG
    TTTGAGTTGA GCTGCACTCT GCCCCTGGAG AAGGACCTGA AGGTCACACT CTATGACTAC
    GACCTCCTGT CCAAGGATGA GAAGATTGGG GAGACCGTCA TCGACCTGGA GAATCGGTTC
    CTGTCGAAAT ATGGGGCGCG CTGTGGGCTT CCCCAGACCT ACTGCATCTC TGGGCCCAAC
    CAGTGGCGAG ACCAGCTCCG TCCCTCCCAA CTGCTCCACC TCTTCTCCCT GCAGCACCAC
    TACAAGGCTC CCACCTACAC GTCCGACCGG CTCATCTTCC GGGAGCAGGA GTACATCCTC
    TCCGAGCTGG AGGATGGGAA ACCCCCCAAC CCTCACCTGG GGCCGGCAGA GGAGCGGCTG
    GCCCTGCATG CACTGCGGAA ACAAGGGCTG GTGCCGGAGC ACGTGGAGAC CCGGCCCCTC
    TACAGCCCCC TGCAGCCAGA GATCGAGCAG GGAAAGCTAC AGATGTGGGT GGACCTGTTT
    CCGAAATCCT TGGGTCAGCC TGGCCCCCCT TTCAACATCA CACCACGCAA AGCCAAGAGG
    TTCTACTTGC GCTGCATCAT CTGGAACACC AGGGACGTCA TCCTCGATGA CCTCAGCATC
    ACTGGGGAGA AGATGAGTGA CATCTATGTC AAAGGCTGGC TGGTTGGCCA CGAGGAGAAC
    AAGCAGAAGA CAGACGTGCA CTACAGGTCG ATGGGAGGAG AAGGCAACTT CAACTGGAGG
    TTCATCTTCC CCTTCGACTA TCTCCCTGCC GAGCAGATGT GTTGCGTTGC AAAGAAGGAG
    CATTTCTGGA GCTTGGATAA GACGGAAAAC AAGATTTCAC CGCAGCTGAT CTTCCAGATC
    TGGGACAATG ACAAGTTCTC CTTCGATGAC TATTTGGGCT CCATCCAGAT GGATCTCAAC
    AGGATGCCCA AGCCTGCCAA GACTGCTGAG AAGTGCTCCT TAGAGCTCAT AGATGACAGC
    TTGTCTGCAA GCCGCTTTGT GTCCCTCTTT GAGCAGAAGA CTGTCAAGGG CTGGTGGCCC
    TGCATTGCAG AGCAGGATGA GAAGAAGATC CTGGCAGGAA AACTGGAAAT GACTTTGGAA
    ATCGTGGCTG AACACGAGCA TGAAGAGCGG CCAGCTGGCG TGGGTCGGGA TGAGCCAAAC
    ATGAACCCCA AGCTGGAGGA CCCCAAGCGC CCAGAGACCT CCTTCCTGTG GTTCACCTCC
    CCGTACAAGA CCCTGAAGTA CATCCTGTGG CGCCGGTACA AGTGGCTGCT CATCCTGGCC
    ATCGTGCTCT TTGTTTTGCT GCTCTTCCTT GGGATCTTCA TCTACGCCTT CCCGAACTAC
    GCTGCTATGA AGCTGGTGAA ACCTTTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015141556.1
    CDS5..6217
    Translation

    Target ORF information:

    RefSeq Version XM_015286070.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus dysferlin (DYSF), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015286070.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGCGCT GCGTGCTGCT GCGCGCCCAA CTGCGCCGCC CCGATAAGGG GGACCGGCAG 
    AGCGACCCCG TGGCCAGCCT GACCTTCCGA GGGGTGAAGA AGAAGACGAA GGTCATCAAG
    AACAGCATCA ACCCCGTATG GAACGAGGGC TTTGAATGGG ATCTGAAGGG CGTTGCTTTG
    GACCTGGGAT CTGAGCTCTG TGTTGTGGTT AAGGACCATG AGACTGTGGG GAGGAATCGG
    TTTTTGGGAG AGGCCCGCGT CCCCCTACGG GATGTGCTGT CCTCCCCCAG CCTGGCGGCC
    TCCTATGATG TCCCATTGCT GGATAGCAAG AAGCAGAGCA CCGGGGCTTC CCTCATCCTA
    CAAGTGTCCT ACAGCCCTCC TCCGGGCGCT GCCCCCCTCT TCCCTCCCCC AACCCCCCCG
    AGGCCGCCCC GGCGGCGGTG GCAGAGTCCG ACACTCTCAC TGCAACCTCC CTGCCTCCCG
    CCCCGCGCAG AAGTGGCCAC CGAGGAGGAG GGGGAGGACC AGGCGGTGAC GGGGGACGAG
    ACGGAGCCCC CATCCTCGGG GCCGCCGGGC TCGGAGCCGC CCTCGGTGCC CCACAGACCC
    CCGGCTCCCC GCGGCCGTGG GGTGAAGCGC AGGAGGAGCT CGCCCAAAAA GCCGCTGTCC
    AACAAACCTC AGGACTTCCA GATCCGGGTT CGGGTGATCG AGGCCCGGCA GCTGCCCGGC
    GTTAACATCC GGCCCGTGGT GAAGGTGACG GCGGCGGGGC AGACCAAGCG GACCAGGATA
    CGCAAAGGCA ACAGCCCCCT CTTCGACGAG ACCTTCTTCT TCAACGTCTT CGAGTCGCCA
    GCCGAGCTGT TCGACGTGCC CGTCTTCATC ACGGTCCTGG ACTCGCGCTC CTTCCGCATG
    GATGCGGTGA TCGGAGAGTT TCGGATGGAC GTTGAGAGCA TCTATTCGGA GCCGAAACAC
    GCGATCCTCA GGCGGTGGCT GCTGCTCTCT GACCCGGAGG ACTTCACGGC GGGGGCAAAG
    GGGTACCTGA AGGTCAGCCT GCTTGTGCTG GGCCCCGGGG ACGAAGCTCC TGTGGAGAAG
    AAGGACGTCT CTGAAGACAA GGAAGACATT GAGGGGAACC TCCTGCGACC CACGGGGGTC
    ACCTTGCGAG GGGCTCAGTT CTGCCTGAAG ATCTTCAAGG CTGAGGATTT GCCCCAGATG
    GACGACGCCG TCATGGACAA CGTCCGGCAG ATCTTCGGCT TCGAAAGCAA CAAGAAGAAC
    CTGGTTGACC CCTTTGTGGA AGTCAGCTTT GCGGGGAAGA CGCTCTACTC GAGGATCCTG
    GAGAAGAATG CCAACCCGCA GTGGAATCAG TGCCTGATGC TGCCGGTGAT GTTCCCTTCC
    ATGTGCGAGA AGATGAGGAT CCGGGTGACC GACTGGGACC GTCTCACACA CAACGACGTC
    GTCGGCACCG CCTACCTCTG CATGTCCAAG ATCTCTGCGC CCGGCGGGGA GCTGGAGGTG
    GACGACGGGC TGGGCTTCCT GCCGACCTTC GGCCCCTGCT ACGTCAACCT GTACGGCAGC
    CCCCGTGAGT TCACCGGCTT CCCGGATCCG TACGAGGCGC TCAATTTAGG AAAGGGCGAG
    GGCGTCGCGT ACCGCGGCCG GGTGCTGCTG GAGCTGGAGA CCAAGCTGGC GGAGCACGGG
    GAGCTGAAGG TGGAGGACAT CTGTGCGGAT GACATCCTGC GGGTGGAGAA GTACCTGCGG
    CGGAGGAAGT ACTCCCTCTT TGCTGCCTTC TACTCGGCCA CCATGCTGCA GGACGTGGAC
    GATGCCATCC AGTTTGAGGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CAACACCTGC
    TTGCCCTTGG CCTCCACCAC GCAGTACAGC CGGGCCGTGT TCGATGGTTG CCAGTATTAC
    TACCTGCCCT GGGGCAACGT GAAGCCCGTG GTGGTGCTGT CCTCCTATTG GGAAGATATC
    AGCTACCGAA CCGACACTCA GAACCTCCTC CAGCACGCAG CAGACAGGCT GGAAGCCAAC
    CTGGAGCGGG TCCACCTCGC GCTCAAGGCC AACGGCGCGC CGGGCGAGCT GGAGTCCCTG
    GCTGCCCAGC TGATGGACGA CGTGATTGCA GACTGCAGTG TTCCGCTGCC CGACGTGCTG
    GGGAAGGCGG CCAGCACACA CCTGGACCAG AACCTGTACC GCTTCCGCAG CACCAACCTG
    GAGCAGATGG TGCGGGCGGC CCTGAGGCTG AAGCACGAGG AGCTGAGCCT GCCGGCCGTG
    CTGGAGCAGG CGGAGGACTG GCTGTGCCGG CTGCGTGCCA TGGCTGAGGA GCCCCAGAAC
    AGCCTCCCAG ACGTGGTGAT CTGGATGCTG CGGGGTGAGA AGCGCGTTGC CTACGCCCGC
    GTGCCGGCCC GCGAGGTGCT CTACTCCCAA AGTGGCCCCA ACTGCTGCGG TAGGAACTGT
    GGCAAGCTGC AGACCATCTT CCTGAAGTAC CCCCAGGAGG AGGCGATGGG GCCGCGCATC
    CCAGCCCAGA TCCGCGTCCA GCTCTGGTTT GGGCTCTCTG TCAATGAGAA GGAGTTCAAC
    CAATACGCTG AGGGGAAGCT CTCCGTCTTT GCTGAAACCT ACGAGAACCA GACCAAGCTG
    GCGCTGGTGG GGAACTGGGG CACCACCGGC CTGACTTACC CCAAATACTC CGATGTCACC
    GGCAAGATCA AGCTCCCCAA GGACAGCTTC CGGCCCTCCA CGGGCTGGAC CTGGGCTGGT
    GATTGGTTCA TCTGCCCAGA GAAGACGATG CTGCACGAGG CAGACGCGGG GCACCTGAGC
    TTCGTGGAGG AGGTGTTTGA GAACCAGGTG CGGCTGCCGG GCGGGCAGTG GATCCACATG
    TCCGATGCCT ACACCGATGT GAATGGGGAG AAGGTTCTGC CCAAGGATGA GATCGAGTGT
    CCTCCGGGCT GGAAATGGGA AGACGTGGAG TGGGACACTG ACCTCAACAG AGCCGTGGAT
    GAGAAAGGCT GGGAGTACGG CATCACCATC CCTCCGGACC GCCGGCCCAA GGCCTGGGTG
    CCAGCTGAGA AGATGTACCA CACCAACCGG CGCCGGCGCT GGGTGCGACT GCGCAGCAGG
    GACCTGGCCC AGATGGAGAC TGTCAGGAAG CACAAGCAGG ACGAGCAGGA CGGCGAGGGC
    TGGGAATACG CCTCGCTGCT GGGCTGGCGC TTCCACCTGC GGCCGCGTCG CACCGATGCC
    TTCCGCCGCC GCCGTTGGAG ACGTAGGATG GAGCCCTTGG AGCGCACCGG GGCTGCTGCT
    GTGTTCGCCT TGGAGGGGGC CCTGGGAGGA ATGACGGACG ACAAAAGCGA CGATGGCAAA
    TCCGTCTCCA CGCTGAGTTT CGGCGTCAAC AGACCCACCA TCTCCTGCAT CTTCGACTAT
    GGGAACCGCT ACCACCTGCG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCCATGGAC
    AAGGACAGCT TCTCGGACCC CTATGCCATC GTTTCCTTCC TCCACCAAAG CCAGAAGACG
    GTGGTGATCA AGAACACCCT GAACCCCACG TGGGACCAGA CGCTCATCTT CTACGAGATA
    GAGATCTTCG GAGACCCCAA GAACGTGTCT GACTGCCCTC CCAGCATCGT GGTGGAGATA
    TACGACCACG ACACCTACGG CGCTGATGAG TTCATGGGGC GCTGCGTGTG CCGGCCCAGC
    CTGCAGCGCG CGCCGCGCCT CTCCTGGCAC CCCGTGCTCA AAGGCGGCAG GAACGTCGGC
    GAGCTGCTGG CTGCCTTCGA GCTCATCCAG AGGGAGAAGC CGGCTGTCCA CCACATCCCT
    GGCTTCGAGA ACGAGCTGTC CTCCGCTCTG GATGAGTCTG AGGACTCTGA CCTGCCCTAC
    CCACCGCCCC AACGGGAACC CAACATCTTC ATGGTCCCCC AAGGGATCAA ACCGGTCCTG
    CAGCGCACGG CCATCGAGAT CCTGGCCTGG GGGCTGAGGA ACCTCAAGAG CTACCAGCTG
    GCCAGCGTCA CCTCACCCAG CCTGCTGGTG GAGTGCGGGG GGCAGCTCGT GCAGTCCTGT
    GTCATCAAGA ACGTCAAGAA AAACCCCAAC TTTGATGTCT GTGTGCTCTT CATGGAAGTG
    CGTCTGCCCA GCGAGAGCCT CTACAGTCCC CCCATCATCA TCAAAATCAT CGACAACCGT
    CCGTTCGGCC GCAGGCCCGT GGTGGGGCAG TGCACCATCC GCTCACTGGA GGAATTCTAC
    TGTGACCCCT ATGGTGAGGA GACGGATGGT GCCCAGGAGC AGGCAGATGA TGTGAGCCTC
    ACGCCCAGGG ATGATGTCCT CATTGACATT GATGACAAAG AGCCTCTCCT TCCCACCCAG
    GAGGAAGAAT TCATTGACTG GTGGAGCAAA TTCTACGCTT CCACAGGAGA GAGGGAGAAG
    TGTGGCTCCT ACTTGGAGAA GGGCTTTGAC ACCCTGCAGG TCTATGAGAC GGAGCTGGAG
    CGCGTGGAGG CCTTCGAGCA GCTCTCTGAC TTCTGCCACA CCTTCAGGCT GTACCGTGGC
    CGGGTGCAGG ATGCCAACGA TGATCCCTCA GTTGTGGGCG AGTTCAAGGG CTCCTTCAAA
    ATCTACCCTC TGCCCGATGA CCCACGCGTG CCGCTGCCGC CGCGGCAGTT CCACCAACTG
    CCAGCCAGGG GTCCCCAGGA GTGTCTCGTC AGGGTCTACA TCATCCGTGC CTTCGGTCTG
    CAGCCCAAGG ATGCCAATGG GAAGTGCGAT CCGTATGTGA AGATTTCAGT GGGAAAGAAA
    TCCATAAACG ACCAAGAAAA TTACCTCCCC TCTACGCTGG AGCCTGTCTT TGGAAAGATG
    TTTGAGTTGA GCTGCACTCT GCCCCTGGAG AAGGACCTGA AGGTCACACT CTATGACTAC
    GACCTCCTGT CCAAGGATGA GAAGATTGGG GAGACCGTCA TCGACCTGGA GAATCGGTTC
    CTGTCGAAAT ATGGGGCGCG CTGTGGGCTT CCCCAGACCT ACTGCATCTC TGGGCCCAAC
    CAGTGGCGAG ACCAGCTCCG TCCCTCCCAA CTGCTCCACC TCTTCTCCCT GCAGCACCAC
    TACAAGGCTC CCACCTACAC GTCCGACCGG CTCATCTTCC GGGAGCAGGA GTACATCCTC
    TCCGAGCTGG AGGATGGGAA ACCCCCCAAC CCTCACCTGG GGCCGGCAGA GGAGCGGCTG
    GCCCTGCATG CACTGCGGAA ACAAGGGCTG GTGCCGGAGC ACGTGGAGAC CCGGCCCCTC
    TACAGCCCCC TGCAGCCAGA GATCGAGCAG GGAAAGCTAC AGATGTGGGT GGACCTGTTT
    CCGAAATCCT TGGGTCAGCC TGGCCCCCCT TTCAACATCA CACCACGCAA AGCCAAGAGG
    TTCTACTTGC GCTGCATCAT CTGGAACACC AGGGACGTCA TCCTCGATGA CCTCAGCATC
    ACTGGGGAGA AGATGAGTGA CATCTATGTC AAAGGCTGGC TGGTTGGCCA CGAGGAGAAC
    AAGCAGAAGA CAGACGTGCA CTACAGGTCG ATGGGAGGAG AAGGCAACTT CAACTGGAGG
    TTCATCTTCC CCTTCGACTA TCTCCCTGCC GAGCAGATGT GTTGCGTTGC AAAGAAGGAG
    CATTTCTGGA GCTTGGATAA GACGGAAAAC AAGATTTCAC CGCAGCTGAT CTTCCAGATC
    TGGGACAATG ACAAGTTCTC CTTCGATGAC TATTTGGGCT CCATCCAGAT GGATCTCAAC
    AGGATGCCCA AGCCTGCCAA GACTGCTGAG AAGTGCTCCT TAGAGCTCAT AGATGACAGC
    TTGTCTGCAA GCCGCTTTGT GTCCCTCTTT GAGCAGAAGA CTGTCAAGGG CTGGTGGCCC
    TGCATTGCAG AGCAGGATGA GAAGAAGATC CTGGCAGGAA AACTGGAAAT GACTTTGGAA
    ATCGTGGCTG AACACGAGCA TGAAGAGCGG CCAGCTGGCG TGGGTCGGGA TGAGCCAAAC
    ATGAACCCCA AGCTGGAGGA CCCCAAGCGC CCAGAGACCT CCTTCCTGTG GTTCACCTCC
    CCGTACAAGA CCCTGAAGTA CATCCTGTGG CGCCGGTACA AGTGGCTGCT CATCCTGGCC
    ATCGTGCTCT TTGTTTTGCT GCTCTTCCTT GGGATCTTCA TCTACGCCTT CCCGAACTAC
    GCTGCTATGA AGCTGGTGAA ACCTTTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.