agla gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009298484.3	XP_009296759.1	glycogen debranching enzyme isoform X1
XM_005163617.2	XP_005163674.1	glycogen debranching enzyme isoform X2
XM_009298483.2	XP_009296758.1	glycogen debranching enzyme isoform X1
XM_005163618.2	XP_005163675.1	glycogen debranching enzyme isoform X1
XM_009298484.2	XP_009296759.1	glycogen debranching enzyme isoform X1
XM_005163617.3	XP_005163674.1	glycogen debranching enzyme isoform X2
XM_009298483.3	XP_009296758.1	glycogen debranching enzyme isoform X1
XM_009298485.2	XP_009296760.1	glycogen debranching enzyme isoform X1
XM_005163618.3	XP_005163675.1	glycogen debranching enzyme isoform X1
NM_001172653.1	NP_001166124.1	glycogen debranching enzyme
XM_009298485.3	XP_009296760.1	glycogen debranching enzyme isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03578	NM_001172653.1 Latest version!	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa06902	XM_005163617.3 Latest version!	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa06903	XM_009298484.3 Latest version!	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa06903	XM_005163618.3 Latest version!	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa06903	XM_009298483.3 Latest version!	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa06903	XM_009298485.3 Latest version!	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa06903	XM_009298484.2	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa06902	XM_005163617.2	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa06903	XM_009298483.2	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa06903	XM_005163618.2	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa06903	XM_009298485.2	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

agla ( NM_001172653.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001166124.1 Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), mRNA.

Price & Availability↑

CloneID	ODa03578
Clone ID Related Accession (Same CDS sequence)	NM_001172653.1
Accession Version	NM_001172653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Danio rerio(zebrafish)
Product	glycogen debranching enzyme
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX510644.3 and CU928067.8. On Jun 2, 2011 this sequence version replaced XM_691102.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGCC 
AAACCAGACA GCAGCAAGAC TGATGGCAAA AAGCAGCTGA AGATCATTCA AGACCCTCAG 
TACAGACGCT TTGGTAACAC AGTGGACATG AACACGGCTC TAGAACTCTT TGTGCCCAAT 
GGGAACAGTC CTGGTGCCAT TGAAGACTGC TGTAATTGGC TAAGGAGGAA ACTGGAGGAA 
CTCAATGGGG AGCAGTTTCA TGAAGTAAAG CACCACCAGG AGCAGGCTAC TAATTGCATT 
GATGGAACAG TAAGCTATGA GAGACTGGCT GACCATGGAC CCAAACTGGG CCCCATCACC 
AGGAAACATC CACTGGTTAC CAGATATTTC ACTTTCCCAT TCGAGGAGAC AACTTTGGAG 
CAAGATCTGG AGCTGATTAA TCAACCAGAG AAGTCCTGTC ACTTCCTGGC CCACAACGGG 
TGGGTCATGG GGGACGATCC CTTGAGGAAC TTCGCTGAGC CAGGCTCCAA CGTCTACATC 
AGGAGGGAGC TGATCTGCTG GGGCGACAGC GTTAAACTGC GCTATGGCAG CGGGCCCGAG 
GACTGCCCCT ACCTGTGGGC CCACATGCAA AAATACACCG AAATCACTGC CAAGCACTTT 
GTGGGAGTGC GTCTGGACAA CTGCCATTCA ACTCCACTGC ATGTGGCTGA GTTCATGCTG 
GATGCCGCCC GTAGACTCAG GCCTGACCTG TATGTGGTGG CTGAGCTCTT CACTGGCAGC 
GAGGAGCTGG ATAATGTCTT TGTGACTAGA TTAGGAATCA CTTCACTTAT CAGAGAGGCG 
ATGAGTGCAG GAGACAGTCA TGAGGAGGGC AGGCTGGTCT ATCGGTATGG AGGAGAACCA 
GTGGGAGCTT TTCTTCAGCC CAGTCTCAGG CCTCTGGTGC CCAGCATTGC CCACGCAATG 
TTTCTGGACG TCACACATGA CAACGAGTGC CCCATTCAGA TCCGCTCTGT GTATGATTCG 
CTGCCCAGCT CTGCTATTGT TTCCATGGCC TGCTGTGCCA CTGGCAGCAC CAGAGGATAT 
GATGAGTTGG TGCCACACCA GATCTCAGTG GTATCTGAGG AGCGTTTCTA TACCAAGTGG 
AGTTCTCAAG CCAAGCCCTC GTCTCCTAAT GAGGTCAACC TGCAGACAGG AATCATTGCT 
GGAAAGCTGG CTCTCAATCG GCTACACCAG GAGCTGGCAG CTAAAGGCTT CAGCCAGGTG 
TTTGTGGACC AGGTGGATGA GGACATTGTT GCAGTGACTC GTCATTGTCC CAGCACACAC 
CAGTCAGTTG TGGCTGTGTG TCGCACTGCG TTCAAGAACC CCAAACACCA TCGCTATACA 
GACGAAGTTC CACCAATGTG CATTCCAGGT AAGATAGAGG AGGTGGTGCT GGAGGCGCGG 
ACCGTGGAGA GGCAGACGGG CACCTACAAA AAAGATGAGA GGAGCATTAA TGGCATGCCT 
GAGTACACGG TGGAGATTAA AGAGCACATC CAGCTGGCAG ACAGTAAGGT GGTCCGTCAG 
GCTGGAGTGA CGTCTCGAGG TCGCAGCGAA TACGTGCAGG AGATTGTGTT TGAGAAGCTG 
ACACCTGGAA GCGTTATTGC CTTCAGGGTG AGTCTGGACC CCAAATCCCA GGAGCTGGTT 
GGTGTTTTGA GAAACCATCT CATTCAGTTT AATCCTCTGT ATAAAGCAGG AAGCGTGCCT 
GATTCAAACA CACCTGACAT ACTGAAGAGT CCGCTGACAC AGATTATGTC TAAGTTGACT 
CTGGCCGATA TGAATCTTCT GCTGTTTCGT TGTGATTCAG AGGAAAAAGA AGATGGAGGC 
GGCTGCTACA ACATCCCCGG CTGGACACCA CTTAAATACG GTGGTTTACA AGGTCTGTTG 
TCAGTGATGG CAGAGATTCG ACCCAAAAAT GATCTTGGCC ACCCGTTCTG TGACAACCTA 
AGGCAAGGGG ATTGGATGAT AGATTATGTG AGCAATAGAT TGGTGTCTCG CGGGGGCCCG 
CTGGCTGAGG TGGGTCAGTG GTTTCAGGCT ATGTTTGCTT ACTTGAAGCA CATTCCCCGC 
TACCTCATTC CCTGTTACTT TGATGCCATC CTGGTTGGGG CCTTCACCAC TGCCCTTGAT 
GCCACTTTCA GACAGATGTC AAGTTTTGTG CAAGATGGCT CATCTTTTGT CAAGTTGCTG 
GCTCTGGGAT CAGTCCAGAT GTGTGGTGTT GGACGTTTCC CAGCTCTTCC ACCTCTCTCG 
CCAGCCCTAA GCGACGTGCC TTACCGGGTC AATGAAATCA CTAAGGAAAA GGAGCAGTGC 
TGTGTCTCTA TGGCGGCAGG CCTGCCGCAT TTCTCCGCCG GAATTTTCCG TTGTTGGGGC 
AGGGATACCT TCATTGCCCT GCGAGGACTA CTACTGGTCA CCGGCCGTCA CCTTGAGGCC 
AGGAACATCA TCTTGGCCTT TGCTGGCACA TTGAGACATG GCTTGATCCC CAACCTGCTG 
GGTGAGGGGG TAGGGGCCCG CTTTAATTGT CGTGATGCAG TGTGGTGGTG GCTACAGTGC 
ATTCAGGACT ACTGTACCAT GGTGCCCAAT GGTACTGACA TCCTTCAGTG CCCTGTGTCC 
CGAATGTACC CCACTGATGA CTCGAAGCCC CTGACAGCTG GCACTGTGGA TCAGCTACTG 
TATGAGGTTA TTCATGAGGC CATGGAGCGC CACATACAGG GTATAGAGTT CAGAGAGAGC 
AATGCCGGAC CACAGATTGA TCGTAACATG AGAGACGAAG GTTTCAATAT AGAGGCCAAG 
GTGAACCCTG ACACTGGCTT TGTGTATGGT GGAAATCGGT TCAACTGTGG GACCTGGATG 
GACAAAATGG GAGAGAGTGA GAGGGCCCGC AACAAAGGGA TACCGGCTAC ACCCAGAGAT 
GGATCAGCGG TGGAAATTGT GGGTCTGTGC AAATCTACCT TACGCTGGCT GGTGGAGCTC 
CATAAAAAAG GGCTCTTTCC TTATAATACT GTGAAAATAC AGAGAGACGG GAAACAGCTC 
TCTGTGGCCT ATGATGAATG GGAAATGAAA ATCCAGAAGA ACTTTGAGAA GATGTTCTAT 
GTGTCTCATG ACCCGAATGA CCCTAACGAG AAGCATCCTG AGCTGGTCCA TAAGCGTGGC 
ATTTACAAAG ACAGCTATGG AGCCTCCAGC CCCTGGTGTG ATTACCAGCT CAGACCCAAC 
TTTACCATAG CCATGGTGGT GGCTCCAGAG CTGTTTACTG TGGAGCATGC CTGGAAAGCG 
CTGAACATGG CAGAAGAGAA GCTGCTCGGA CCTCTTGGGA TGAAGACTTT AGACCCAGAT 
GACATGGTTT ACTGTGGCGT TTATGACAAT TCGCTGGACA ATGACAACTA CAACCTCGCC 
AAAGGCTTCA ACTATCATCA GGGCCCTGAG TGGCTGTGGC CTATTGGATA CTTTCTCCGC 
GCAAAGATCT TCTTTGCAAA GAAAATGGGC AAAGACACTT ATGACAAAAC TGTGTATTTG 
GTGAAGAATG TTCTCTCTCG CCATAATGTC CACATGGAAA GATCATCCTG GAAGGGTCTT 
CCGGAGCTCA CTAATGAGAA CGGGCAGTAC TGCTCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCTCTCGCCG CAGTGCTCGA GGTGCTTCAT GATCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166124.1
CDS	127..4725
Translation	MTAKQTRVMVLNDMEKLDRTLFRLDQGFELQFRLGPTLQGKHVRVYTNYPTEGERFDRQKFHALDWINPTAREDDSDKFCTLDLKISGSYQYYFGHGGDEKSGGGYIVVDPVLRVGADNHVLPLDCITIQTYLSKCLGPLDEWSDRLRVAKEAGYNMIHFTPLQTLGESRSCYSLADQLELNPDFSPSGQTYTWTDAGNLVEKLKNEWNMLCITDVVYNHTASNSKWIKKHPECGYNLVNSPHLKPAWVLDRALWHVTCAIADGKYEDRGLPALIQDHPHLHAIRGVLWQEVFPKIKLWEFFQVKIEALVEQFRALLQSAKPDSSKTDGKKQLKIIQDPQYRRFGNTVDMNTALELFVPNGNSPGAIEDCCNWLRRKLEELNGEQFHEVKHHQEQATNCIDGTVSYERLADHGPKLGPITRKHPLVTRYFTFPFEETTLEQDLELINQPEKSCHFLAHNGWVMGDDPLRNFAEPGSNVYIRRELICWGDSVKLRYGSGPEDCPYLWAHMQKYTEITAKHFVGVRLDNCHSTPLHVAEFMLDAARRLRPDLYVVAELFTGSEELDNVFVTRLGITSLIREAMSAGDSHEEGRLVYRYGGEPVGAFLQPSLRPLVPSIAHAMFLDVTHDNECPIQIRSVYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWSSQAKPSSPNEVNLQTGIIAGKLALNRLHQELAAKGFSQVFVDQVDEDIVAVTRHCPSTHQSVVAVCRTAFKNPKHHRYTDEVPPMCIPGKIEEVVLEARTVERQTGTYKKDERSINGMPEYTVEIKEHIQLADSKVVRQAGVTSRGRSEYVQEIVFEKLTPGSVIAFRVSLDPKSQELVGVLRNHLIQFNPLYKAGSVPDSNTPDILKSPLTQIMSKLTLADMNLLLFRCDSEEKEDGGGCYNIPGWTPLKYGGLQGLLSVMAEIRPKNDLGHPFCDNLRQGDWMIDYVSNRLVSRGGPLAEVGQWFQAMFAYLKHIPRYLIPCYFDAILVGAFTTALDATFRQMSSFVQDGSSFVKLLALGSVQMCGVGRFPALPPLSPALSDVPYRVNEITKEKEQCCVSMAAGLPHFSAGIFRCWGRDTFIALRGLLLVTGRHLEARNIILAFAGTLRHGLIPNLLGEGVGARFNCRDAVWWWLQCIQDYCTMVPNGTDILQCPVSRMYPTDDSKPLTAGTVDQLLYEVIHEAMERHIQGIEFRESNAGPQIDRNMRDEGFNIEAKVNPDTGFVYGGNRFNCGTWMDKMGESERARNKGIPATPRDGSAVEIVGLCKSTLRWLVELHKKGLFPYNTVKIQRDGKQLSVAYDEWEMKIQKNFEKMFYVSHDPNDPNEKHPELVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTVEHAWKALNMAEEKLLGPLGMKTLDPDDMVYCGVYDNSLDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKIFFAKKMGKDTYDKTVYLVKNVLSRHNVHMERSSWKGLPELTNENGQYCSFSCETQAWSLAAVLEVLHDL

Target ORF information:

RefSeq Version	NM_001172653.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172653.1

ORF Insert Sequence:

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGCC 
AAACCAGACA GCAGCAAGAC TGATGGCAAA AAGCAGCTGA AGATCATTCA AGACCCTCAG 
TACAGACGCT TTGGTAACAC AGTGGACATG AACACGGCTC TAGAACTCTT TGTGCCCAAT 
GGGAACAGTC CTGGTGCCAT TGAAGACTGC TGTAATTGGC TAAGGAGGAA ACTGGAGGAA 
CTCAATGGGG AGCAGTTTCA TGAAGTAAAG CACCACCAGG AGCAGGCTAC TAATTGCATT 
GATGGAACAG TAAGCTATGA GAGACTGGCT GACCATGGAC CCAAACTGGG CCCCATCACC 
AGGAAACATC CACTGGTTAC CAGATATTTC ACTTTCCCAT TCGAGGAGAC AACTTTGGAG 
CAAGATCTGG AGCTGATTAA TCAACCAGAG AAGTCCTGTC ACTTCCTGGC CCACAACGGG 
TGGGTCATGG GGGACGATCC CTTGAGGAAC TTCGCTGAGC CAGGCTCCAA CGTCTACATC 
AGGAGGGAGC TGATCTGCTG GGGCGACAGC GTTAAACTGC GCTATGGCAG CGGGCCCGAG 
GACTGCCCCT ACCTGTGGGC CCACATGCAA AAATACACCG AAATCACTGC CAAGCACTTT 
GTGGGAGTGC GTCTGGACAA CTGCCATTCA ACTCCACTGC ATGTGGCTGA GTTCATGCTG 
GATGCCGCCC GTAGACTCAG GCCTGACCTG TATGTGGTGG CTGAGCTCTT CACTGGCAGC 
GAGGAGCTGG ATAATGTCTT TGTGACTAGA TTAGGAATCA CTTCACTTAT CAGAGAGGCG 
ATGAGTGCAG GAGACAGTCA TGAGGAGGGC AGGCTGGTCT ATCGGTATGG AGGAGAACCA 
GTGGGAGCTT TTCTTCAGCC CAGTCTCAGG CCTCTGGTGC CCAGCATTGC CCACGCAATG 
TTTCTGGACG TCACACATGA CAACGAGTGC CCCATTCAGA TCCGCTCTGT GTATGATTCG 
CTGCCCAGCT CTGCTATTGT TTCCATGGCC TGCTGTGCCA CTGGCAGCAC CAGAGGATAT 
GATGAGTTGG TGCCACACCA GATCTCAGTG GTATCTGAGG AGCGTTTCTA TACCAAGTGG 
AGTTCTCAAG CCAAGCCCTC GTCTCCTAAT GAGGTCAACC TGCAGACAGG AATCATTGCT 
GGAAAGCTGG CTCTCAATCG GCTACACCAG GAGCTGGCAG CTAAAGGCTT CAGCCAGGTG 
TTTGTGGACC AGGTGGATGA GGACATTGTT GCAGTGACTC GTCATTGTCC CAGCACACAC 
CAGTCAGTTG TGGCTGTGTG TCGCACTGCG TTCAAGAACC CCAAACACCA TCGCTATACA 
GACGAAGTTC CACCAATGTG CATTCCAGGT AAGATAGAGG AGGTGGTGCT GGAGGCGCGG 
ACCGTGGAGA GGCAGACGGG CACCTACAAA AAAGATGAGA GGAGCATTAA TGGCATGCCT 
GAGTACACGG TGGAGATTAA AGAGCACATC CAGCTGGCAG ACAGTAAGGT GGTCCGTCAG 
GCTGGAGTGA CGTCTCGAGG TCGCAGCGAA TACGTGCAGG AGATTGTGTT TGAGAAGCTG 
ACACCTGGAA GCGTTATTGC CTTCAGGGTG AGTCTGGACC CCAAATCCCA GGAGCTGGTT 
GGTGTTTTGA GAAACCATCT CATTCAGTTT AATCCTCTGT ATAAAGCAGG AAGCGTGCCT 
GATTCAAACA CACCTGACAT ACTGAAGAGT CCGCTGACAC AGATTATGTC TAAGTTGACT 
CTGGCCGATA TGAATCTTCT GCTGTTTCGT TGTGATTCAG AGGAAAAAGA AGATGGAGGC 
GGCTGCTACA ACATCCCCGG CTGGACACCA CTTAAATACG GTGGTTTACA AGGTCTGTTG 
TCAGTGATGG CAGAGATTCG ACCCAAAAAT GATCTTGGCC ACCCGTTCTG TGACAACCTA 
AGGCAAGGGG ATTGGATGAT AGATTATGTG AGCAATAGAT TGGTGTCTCG CGGGGGCCCG 
CTGGCTGAGG TGGGTCAGTG GTTTCAGGCT ATGTTTGCTT ACTTGAAGCA CATTCCCCGC 
TACCTCATTC CCTGTTACTT TGATGCCATC CTGGTTGGGG CCTTCACCAC TGCCCTTGAT 
GCCACTTTCA GACAGATGTC AAGTTTTGTG CAAGATGGCT CATCTTTTGT CAAGTTGCTG 
GCTCTGGGAT CAGTCCAGAT GTGTGGTGTT GGACGTTTCC CAGCTCTTCC ACCTCTCTCG 
CCAGCCCTAA GCGACGTGCC TTACCGGGTC AATGAAATCA CTAAGGAAAA GGAGCAGTGC 
TGTGTCTCTA TGGCGGCAGG CCTGCCGCAT TTCTCCGCCG GAATTTTCCG TTGTTGGGGC 
AGGGATACCT TCATTGCCCT GCGAGGACTA CTACTGGTCA CCGGCCGTCA CCTTGAGGCC 
AGGAACATCA TCTTGGCCTT TGCTGGCACA TTGAGACATG GCTTGATCCC CAACCTGCTG 
GGTGAGGGGG TAGGGGCCCG CTTTAATTGT CGTGATGCAG TGTGGTGGTG GCTACAGTGC 
ATTCAGGACT ACTGTACCAT GGTGCCCAAT GGTACTGACA TCCTTCAGTG CCCTGTGTCC 
CGAATGTACC CCACTGATGA CTCGAAGCCC CTGACAGCTG GCACTGTGGA TCAGCTACTG 
TATGAGGTTA TTCATGAGGC CATGGAGCGC CACATACAGG GTATAGAGTT CAGAGAGAGC 
AATGCCGGAC CACAGATTGA TCGTAACATG AGAGACGAAG GTTTCAATAT AGAGGCCAAG 
GTGAACCCTG ACACTGGCTT TGTGTATGGT GGAAATCGGT TCAACTGTGG GACCTGGATG 
GACAAAATGG GAGAGAGTGA GAGGGCCCGC AACAAAGGGA TACCGGCTAC ACCCAGAGAT 
GGATCAGCGG TGGAAATTGT GGGTCTGTGC AAATCTACCT TACGCTGGCT GGTGGAGCTC 
CATAAAAAAG GGCTCTTTCC TTATAATACT GTGAAAATAC AGAGAGACGG GAAACAGCTC 
TCTGTGGCCT ATGATGAATG GGAAATGAAA ATCCAGAAGA ACTTTGAGAA GATGTTCTAT 
GTGTCTCATG ACCCGAATGA CCCTAACGAG AAGCATCCTG AGCTGGTCCA TAAGCGTGGC 
ATTTACAAAG ACAGCTATGG AGCCTCCAGC CCCTGGTGTG ATTACCAGCT CAGACCCAAC 
TTTACCATAG CCATGGTGGT GGCTCCAGAG CTGTTTACTG TGGAGCATGC CTGGAAAGCG 
CTGAACATGG CAGAAGAGAA GCTGCTCGGA CCTCTTGGGA TGAAGACTTT AGACCCAGAT 
GACATGGTTT ACTGTGGCGT TTATGACAAT TCGCTGGACA ATGACAACTA CAACCTCGCC 
AAAGGCTTCA ACTATCATCA GGGCCCTGAG TGGCTGTGGC CTATTGGATA CTTTCTCCGC 
GCAAAGATCT TCTTTGCAAA GAAAATGGGC AAAGACACTT ATGACAAAAC TGTGTATTTG 
GTGAAGAATG TTCTCTCTCG CCATAATGTC CACATGGAAA GATCATCCTG GAAGGGTCTT 
CCGGAGCTCA CTAATGAGAA CGGGCAGTAC TGCTCCTTCA GCTGTGAAAC TCAGGCCTGG 
TCTCTCGCCG CAGTGCTCGA GGTGCTTCAT GATCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agla ( XM_005163617.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163674.1 Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa06902
Clone ID Related Accession (Same CDS sequence)	XM_005163617.2 , XM_005163617.3
Accession Version	XM_005163617.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glycogen debranching enzyme isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005163617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGGCCATG 
CTAGCGGCAG CCAGATCAGT CAGACCCAAT CTGTATGTGA TAGCCGAGCT CTTTACAGGC 
AGCGAACTGA TTGACAATGT GTTTGTAAAC CGGCTGGGAA TAACCAGTCT CATAAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163674.1
CDS	162..4763
Translation	MTAKQTRVMVLNDMEKLDRTLFRLDQGFELQFRLGPTLQGKHVRVYTNYPTEGERFDRQKFHALDWINPTAREDDSDKFCTLDLKISGSYQYYFGHGGDEKSGGGYIVVDPVLRVGADNHVLPLDCITIQTYLSKCLGPLDEWSDRLRVAKEAGYNMIHFTPLQTLGESRSCYSLADQLELNPDFSPSGQTYTWTDAGNLVEKLKNEWNMLCITDVVYNHTASNSKWIKKHPECGYNLVNSPHLKPAWVLDRALWHVTCAIADGKYEDRGLPALIQDHPHLHAIRGVLWQEVFPKIKLWEFFQVKIEALVEQFRALLQSGAKPDSSKTDGKKQLKIIQDPQYRRFGNTVDMNTALELFVPNGNSPGAIEDCCNWLRRKLEELNGEQFHEVKHHQEQATNCIDGTVSYERLADHGPKLGPITRKHPLVTRYFTFPFEETTLEQDLELINQPEKSCHFLAHNGWVMGDDPLRNFAEPGSNVYIRRELICWGDSVKLRYGSGPEDCPYLWAHMQKYTEITAKHFVGVRLDNCHSTPLHVAEAMLAAARSVRPNLYVIAELFTGSELIDNVFVNRLGITSLIREAMSAGDSHEEGRLVYRYGGEPVGAFLQPSLRPLVPSIAHAMFLDVTHDNECPIQIRSVYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWSSQAKPSSPNEVNLQTGIIAGKLALNRLHQELAAKGFSQVFVDQVDEDIVAVTRHCPSTHQSVVAVCRTAFKNPKHHRYTDEVPPMCIPGKIEEVVLEARTVERQTGTYKKDERSINGMPEYTVEIKEHIQLADSKVVRQAGVTSRGRSEYVQEIVFEKLTPGSVIAFRVSLDPKSQELVGVLRNHLIQFNPLYKAGSVPDSNTPDILKSPLTQIMSKLTLADMNLLLFRCDSEEKEDGGGCYNIPGWTPLKYGGLQGLLSVMAEIRPKNDLGHPFCDNLRQGDWMIDYVSNRLVSRGGPLAEVGQWFQAMFAYLKHIPRYLIPCYFDAILVGAFTTALDATFRQMSSFVQDGSSFVKLLALGSVQMCGVGRFPALPPLSPALSDVPYRVNEITKEKEQCCVSMAAGLPHFSAGIFRCWGRDTFIALRGLLLVTGRHLEARNIILAFAGTLRHGLIPNLLGEGVGARFNCRDAVWWWLQCIQDYCTMVPNGTDILQCPVSRMYPTDDSKPLTAGTVDQLLYEVIHEAMERHIQGIEFRESNAGPQIDRNMRDEGFNIEAKVNPDTGFVYGGNRFNCGTWMDKMGESERARNKGIPATPRDGSAVEIVGLCKSTLRWLVELHKKGLFPYNTVKIQRDGKQLSVAYDEWEMKIQKNFEKMFYVSHDPNDPNEKHPELVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTVEHAWKALNMAEEKLLGPLGMKTLDPDDMVYCGVYDNSLDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKIFFAKKMGKDTYDKTVYLVKNVLSRHNVHMERSSWKGLPELTNENGQYCSFSCETQAWSLAAVLEVLHDL

Target ORF information:

RefSeq Version	XM_005163617.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163617.2

ORF Insert Sequence:

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGGCCATG 
CTAGCGGCAG CCAGATCAGT CAGACCCAAT CTGTATGTGA TAGCCGAGCT CTTTACAGGC 
AGCGAACTGA TTGACAATGT GTTTGTAAAC CGGCTGGGAA TAACCAGTCT CATAAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agla ( XM_005163617.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163674.1 Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X2, mRNA.

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CloneID	ODa06902
Clone ID Related Accession (Same CDS sequence)	XM_005163617.2 , XM_005163617.3
Accession Version	XM_005163617.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163617.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGGCCATG 
CTAGCGGCAG CCAGATCAGT CAGACCCAAT CTGTATGTGA TAGCCGAGCT CTTTACAGGC 
AGCGAACTGA TTGACAATGT GTTTGTAAAC CGGCTGGGAA TAACCAGTCT CATAAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163674.1
CDS	175..4776
Translation	MTAKQTRVMVLNDMEKLDRTLFRLDQGFELQFRLGPTLQGKHVRVYTNYPTEGERFDRQKFHALDWINPTAREDDSDKFCTLDLKISGSYQYYFGHGGDEKSGGGYIVVDPVLRVGADNHVLPLDCITIQTYLSKCLGPLDEWSDRLRVAKEAGYNMIHFTPLQTLGESRSCYSLADQLELNPDFSPSGQTYTWTDAGNLVEKLKNEWNMLCITDVVYNHTASNSKWIKKHPECGYNLVNSPHLKPAWVLDRALWHVTCAIADGKYEDRGLPALIQDHPHLHAIRGVLWQEVFPKIKLWEFFQVKIEALVEQFRALLQSGAKPDSSKTDGKKQLKIIQDPQYRRFGNTVDMNTALELFVPNGNSPGAIEDCCNWLRRKLEELNGEQFHEVKHHQEQATNCIDGTVSYERLADHGPKLGPITRKHPLVTRYFTFPFEETTLEQDLELINQPEKSCHFLAHNGWVMGDDPLRNFAEPGSNVYIRRELICWGDSVKLRYGSGPEDCPYLWAHMQKYTEITAKHFVGVRLDNCHSTPLHVAEAMLAAARSVRPNLYVIAELFTGSELIDNVFVNRLGITSLIREAMSAGDSHEEGRLVYRYGGEPVGAFLQPSLRPLVPSIAHAMFLDVTHDNECPIQIRSVYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWSSQAKPSSPNEVNLQTGIIAGKLALNRLHQELAAKGFSQVFVDQVDEDIVAVTRHCPSTHQSVVAVCRTAFKNPKHHRYTDEVPPMCIPGKIEEVVLEARTVERQTGTYKKDERSINGMPEYTVEIKEHIQLADSKVVRQAGVTSRGRSEYVQEIVFEKLTPGSVIAFRVSLDPKSQELVGVLRNHLIQFNPLYKAGSVPDSNTPDILKSPLTQIMSKLTLADMNLLLFRCDSEEKEDGGGCYNIPGWTPLKYGGLQGLLSVMAEIRPKNDLGHPFCDNLRQGDWMIDYVSNRLVSRGGPLAEVGQWFQAMFAYLKHIPRYLIPCYFDAILVGAFTTALDATFRQMSSFVQDGSSFVKLLALGSVQMCGVGRFPALPPLSPALSDVPYRVNEITKEKEQCCVSMAAGLPHFSAGIFRCWGRDTFIALRGLLLVTGRHLEARNIILAFAGTLRHGLIPNLLGEGVGARFNCRDAVWWWLQCIQDYCTMVPNGTDILQCPVSRMYPTDDSKPLTAGTVDQLLYEVIHEAMERHIQGIEFRESNAGPQIDRNMRDEGFNIEAKVNPDTGFVYGGNRFNCGTWMDKMGESERARNKGIPATPRDGSAVEIVGLCKSTLRWLVELHKKGLFPYNTVKIQRDGKQLSVAYDEWEMKIQKNFEKMFYVSHDPNDPNEKHPELVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTVEHAWKALNMAEEKLLGPLGMKTLDPDDMVYCGVYDNSLDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKIFFAKKMGKDTYDKTVYLVKNVLSRHNVHMERSSWKGLPELTNENGQYCSFSCETQAWSLAAVLEVLHDL

Target ORF information:

RefSeq Version	XM_005163617.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163617.3

ORF Insert Sequence:

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGGCCATG 
CTAGCGGCAG CCAGATCAGT CAGACCCAAT CTGTATGTGA TAGCCGAGCT CTTTACAGGC 
AGCGAACTGA TTGACAATGT GTTTGTAAAC CGGCTGGGAA TAACCAGTCT CATAAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agla ( XM_009298484.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296759.1 Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X4, mRNA.

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CloneID	ODa06903
Clone ID Related Accession (Same CDS sequence)	XM_009298484.3 , XM_009298483.2 , XM_005163618.2 , XM_009298484.2 , XM_009298483.3 , XM_009298485.2 , XM_005163618.3 , XM_009298485.3
Accession Version	XM_009298484.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298484.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296759.1
CDS	256..4857
Translation	MTAKQTRVMVLNDMEKLDRTLFRLDQGFELQFRLGPTLQGKHVRVYTNYPTEGERFDRQKFHALDWINPTAREDDSDKFCTLDLKISGSYQYYFGHGGDEKSGGGYIVVDPVLRVGADNHVLPLDCITIQTYLSKCLGPLDEWSDRLRVAKEAGYNMIHFTPLQTLGESRSCYSLADQLELNPDFSPSGQTYTWTDAGNLVEKLKNEWNMLCITDVVYNHTASNSKWIKKHPECGYNLVNSPHLKPAWVLDRALWHVTCAIADGKYEDRGLPALIQDHPHLHAIRGVLWQEVFPKIKLWEFFQVKIEALVEQFRALLQSGAKPDSSKTDGKKQLKIIQDPQYRRFGNTVDMNTALELFVPNGNSPGAIEDCCNWLRRKLEELNGEQFHEVKHHQEQATNCIDGTVSYERLADHGPKLGPITRKHPLVTRYFTFPFEETTLEQDLELINQPEKSCHFLAHNGWVMGDDPLRNFAEPGSNVYIRRELICWGDSVKLRYGSGPEDCPYLWAHMQKYTEITAKHFVGVRLDNCHSTPLHVAEFMLDAARRLRPDLYVVAELFTGSEELDNVFVTRLGITSLIREAMSAGDSHEEGRLVYRYGGEPVGAFLQPSLRPLVPSIAHAMFLDVTHDNECPIQIRSVYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWSSQAKPSSPNEVNLQTGIIAGKLALNRLHQELAAKGFSQVFVDQVDEDIVAVTRHCPSTHQSVVAVCRTAFKNPKHHRYTDEVPPMCIPGKIEEVVLEARTVERQTGTYKKDERSINGMPEYTVEIKEHIQLADSKVVRQAGVTSRGRSEYVQEIVFEKLTPGSVIAFRVSLDPKSQELVGVLRNHLIQFNPLYKAGSVPDSNTPDILKSPLTQIMSKLTLADMNLLLFRCDSEEKEDGGGCYNIPGWTPLKYGGLQGLLSVMAEIRPKNDLGHPFCDNLRQGDWMIDYVSNRLVSRGGPLAEVGQWFQAMFAYLKHIPRYLIPCYFDAILVGAFTTALDATFRQMSSFVQDGSSFVKLLALGSVQMCGVGRFPALPPLSPALSDVPYRVNEITKEKEQCCVSMAAGLPHFSAGIFRCWGRDTFIALRGLLLVTGRHLEARNIILAFAGTLRHGLIPNLLGEGVGARFNCRDAVWWWLQCIQDYCTMVPNGTDILQCPVSRMYPTDDSKPLTAGTVDQLLYEVIHEAMERHIQGIEFRESNAGPQIDRNMRDEGFNIEAKVNPDTGFVYGGNRFNCGTWMDKMGESERARNKGIPATPRDGSAVEIVGLCKSTLRWLVELHKKGLFPYNTVKIQRDGKQLSVAYDEWEMKIQKNFEKMFYVSHDPNDPNEKHPELVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTVEHAWKALNMAEEKLLGPLGMKTLDPDDMVYCGVYDNSLDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKIFFAKKMGKDTYDKTVYLVKNVLSRHNVHMERSSWKGLPELTNENGQYCSFSCETQAWSLAAVLEVLHDL

Target ORF information:

RefSeq Version	XM_009298484.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298484.3

ORF Insert Sequence:

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agla ( XM_009298483.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296758.1 Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X2, mRNA.

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CloneID	ODa06903
Clone ID Related Accession (Same CDS sequence)	XM_009298484.3 , XM_009298483.2 , XM_005163618.2 , XM_009298484.2 , XM_009298483.3 , XM_009298485.2 , XM_005163618.3 , XM_009298485.3
Accession Version	XM_009298483.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glycogen debranching enzyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296758.1
CDS	156..4757
Translation	MTAKQTRVMVLNDMEKLDRTLFRLDQGFELQFRLGPTLQGKHVRVYTNYPTEGERFDRQKFHALDWINPTAREDDSDKFCTLDLKISGSYQYYFGHGGDEKSGGGYIVVDPVLRVGADNHVLPLDCITIQTYLSKCLGPLDEWSDRLRVAKEAGYNMIHFTPLQTLGESRSCYSLADQLELNPDFSPSGQTYTWTDAGNLVEKLKNEWNMLCITDVVYNHTASNSKWIKKHPECGYNLVNSPHLKPAWVLDRALWHVTCAIADGKYEDRGLPALIQDHPHLHAIRGVLWQEVFPKIKLWEFFQVKIEALVEQFRALLQSGAKPDSSKTDGKKQLKIIQDPQYRRFGNTVDMNTALELFVPNGNSPGAIEDCCNWLRRKLEELNGEQFHEVKHHQEQATNCIDGTVSYERLADHGPKLGPITRKHPLVTRYFTFPFEETTLEQDLELINQPEKSCHFLAHNGWVMGDDPLRNFAEPGSNVYIRRELICWGDSVKLRYGSGPEDCPYLWAHMQKYTEITAKHFVGVRLDNCHSTPLHVAEFMLDAARRLRPDLYVVAELFTGSEELDNVFVTRLGITSLIREAMSAGDSHEEGRLVYRYGGEPVGAFLQPSLRPLVPSIAHAMFLDVTHDNECPIQIRSVYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWSSQAKPSSPNEVNLQTGIIAGKLALNRLHQELAAKGFSQVFVDQVDEDIVAVTRHCPSTHQSVVAVCRTAFKNPKHHRYTDEVPPMCIPGKIEEVVLEARTVERQTGTYKKDERSINGMPEYTVEIKEHIQLADSKVVRQAGVTSRGRSEYVQEIVFEKLTPGSVIAFRVSLDPKSQELVGVLRNHLIQFNPLYKAGSVPDSNTPDILKSPLTQIMSKLTLADMNLLLFRCDSEEKEDGGGCYNIPGWTPLKYGGLQGLLSVMAEIRPKNDLGHPFCDNLRQGDWMIDYVSNRLVSRGGPLAEVGQWFQAMFAYLKHIPRYLIPCYFDAILVGAFTTALDATFRQMSSFVQDGSSFVKLLALGSVQMCGVGRFPALPPLSPALSDVPYRVNEITKEKEQCCVSMAAGLPHFSAGIFRCWGRDTFIALRGLLLVTGRHLEARNIILAFAGTLRHGLIPNLLGEGVGARFNCRDAVWWWLQCIQDYCTMVPNGTDILQCPVSRMYPTDDSKPLTAGTVDQLLYEVIHEAMERHIQGIEFRESNAGPQIDRNMRDEGFNIEAKVNPDTGFVYGGNRFNCGTWMDKMGESERARNKGIPATPRDGSAVEIVGLCKSTLRWLVELHKKGLFPYNTVKIQRDGKQLSVAYDEWEMKIQKNFEKMFYVSHDPNDPNEKHPELVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTVEHAWKALNMAEEKLLGPLGMKTLDPDDMVYCGVYDNSLDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKIFFAKKMGKDTYDKTVYLVKNVLSRHNVHMERSSWKGLPELTNENGQYCSFSCETQAWSLAAVLEVLHDL

Target ORF information:

RefSeq Version	XM_009298483.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298483.2

ORF Insert Sequence:

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agla ( XM_005163618.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163675.1 Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06903
Clone ID Related Accession (Same CDS sequence)	XM_009298484.3 , XM_009298483.2 , XM_005163618.2 , XM_009298484.2 , XM_009298483.3 , XM_009298485.2 , XM_005163618.3 , XM_009298485.3
Accession Version	XM_005163618.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glycogen debranching enzyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005163618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163675.1
CDS	162..4763
Translation	MTAKQTRVMVLNDMEKLDRTLFRLDQGFELQFRLGPTLQGKHVRVYTNYPTEGERFDRQKFHALDWINPTAREDDSDKFCTLDLKISGSYQYYFGHGGDEKSGGGYIVVDPVLRVGADNHVLPLDCITIQTYLSKCLGPLDEWSDRLRVAKEAGYNMIHFTPLQTLGESRSCYSLADQLELNPDFSPSGQTYTWTDAGNLVEKLKNEWNMLCITDVVYNHTASNSKWIKKHPECGYNLVNSPHLKPAWVLDRALWHVTCAIADGKYEDRGLPALIQDHPHLHAIRGVLWQEVFPKIKLWEFFQVKIEALVEQFRALLQSGAKPDSSKTDGKKQLKIIQDPQYRRFGNTVDMNTALELFVPNGNSPGAIEDCCNWLRRKLEELNGEQFHEVKHHQEQATNCIDGTVSYERLADHGPKLGPITRKHPLVTRYFTFPFEETTLEQDLELINQPEKSCHFLAHNGWVMGDDPLRNFAEPGSNVYIRRELICWGDSVKLRYGSGPEDCPYLWAHMQKYTEITAKHFVGVRLDNCHSTPLHVAEFMLDAARRLRPDLYVVAELFTGSEELDNVFVTRLGITSLIREAMSAGDSHEEGRLVYRYGGEPVGAFLQPSLRPLVPSIAHAMFLDVTHDNECPIQIRSVYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWSSQAKPSSPNEVNLQTGIIAGKLALNRLHQELAAKGFSQVFVDQVDEDIVAVTRHCPSTHQSVVAVCRTAFKNPKHHRYTDEVPPMCIPGKIEEVVLEARTVERQTGTYKKDERSINGMPEYTVEIKEHIQLADSKVVRQAGVTSRGRSEYVQEIVFEKLTPGSVIAFRVSLDPKSQELVGVLRNHLIQFNPLYKAGSVPDSNTPDILKSPLTQIMSKLTLADMNLLLFRCDSEEKEDGGGCYNIPGWTPLKYGGLQGLLSVMAEIRPKNDLGHPFCDNLRQGDWMIDYVSNRLVSRGGPLAEVGQWFQAMFAYLKHIPRYLIPCYFDAILVGAFTTALDATFRQMSSFVQDGSSFVKLLALGSVQMCGVGRFPALPPLSPALSDVPYRVNEITKEKEQCCVSMAAGLPHFSAGIFRCWGRDTFIALRGLLLVTGRHLEARNIILAFAGTLRHGLIPNLLGEGVGARFNCRDAVWWWLQCIQDYCTMVPNGTDILQCPVSRMYPTDDSKPLTAGTVDQLLYEVIHEAMERHIQGIEFRESNAGPQIDRNMRDEGFNIEAKVNPDTGFVYGGNRFNCGTWMDKMGESERARNKGIPATPRDGSAVEIVGLCKSTLRWLVELHKKGLFPYNTVKIQRDGKQLSVAYDEWEMKIQKNFEKMFYVSHDPNDPNEKHPELVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTVEHAWKALNMAEEKLLGPLGMKTLDPDDMVYCGVYDNSLDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKIFFAKKMGKDTYDKTVYLVKNVLSRHNVHMERSSWKGLPELTNENGQYCSFSCETQAWSLAAVLEVLHDL

Target ORF information:

RefSeq Version	XM_005163618.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163618.2

ORF Insert Sequence:

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agla ( XM_009298484.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296759.1 Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa06903
Clone ID Related Accession (Same CDS sequence)	XM_009298484.3 , XM_009298483.2 , XM_005163618.2 , XM_009298484.2 , XM_009298483.3 , XM_009298485.2 , XM_005163618.3 , XM_009298485.3
Accession Version	XM_009298484.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glycogen debranching enzyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296759.1
CDS	147..4748
Translation	MTAKQTRVMVLNDMEKLDRTLFRLDQGFELQFRLGPTLQGKHVRVYTNYPTEGERFDRQKFHALDWINPTAREDDSDKFCTLDLKISGSYQYYFGHGGDEKSGGGYIVVDPVLRVGADNHVLPLDCITIQTYLSKCLGPLDEWSDRLRVAKEAGYNMIHFTPLQTLGESRSCYSLADQLELNPDFSPSGQTYTWTDAGNLVEKLKNEWNMLCITDVVYNHTASNSKWIKKHPECGYNLVNSPHLKPAWVLDRALWHVTCAIADGKYEDRGLPALIQDHPHLHAIRGVLWQEVFPKIKLWEFFQVKIEALVEQFRALLQSGAKPDSSKTDGKKQLKIIQDPQYRRFGNTVDMNTALELFVPNGNSPGAIEDCCNWLRRKLEELNGEQFHEVKHHQEQATNCIDGTVSYERLADHGPKLGPITRKHPLVTRYFTFPFEETTLEQDLELINQPEKSCHFLAHNGWVMGDDPLRNFAEPGSNVYIRRELICWGDSVKLRYGSGPEDCPYLWAHMQKYTEITAKHFVGVRLDNCHSTPLHVAEFMLDAARRLRPDLYVVAELFTGSEELDNVFVTRLGITSLIREAMSAGDSHEEGRLVYRYGGEPVGAFLQPSLRPLVPSIAHAMFLDVTHDNECPIQIRSVYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWSSQAKPSSPNEVNLQTGIIAGKLALNRLHQELAAKGFSQVFVDQVDEDIVAVTRHCPSTHQSVVAVCRTAFKNPKHHRYTDEVPPMCIPGKIEEVVLEARTVERQTGTYKKDERSINGMPEYTVEIKEHIQLADSKVVRQAGVTSRGRSEYVQEIVFEKLTPGSVIAFRVSLDPKSQELVGVLRNHLIQFNPLYKAGSVPDSNTPDILKSPLTQIMSKLTLADMNLLLFRCDSEEKEDGGGCYNIPGWTPLKYGGLQGLLSVMAEIRPKNDLGHPFCDNLRQGDWMIDYVSNRLVSRGGPLAEVGQWFQAMFAYLKHIPRYLIPCYFDAILVGAFTTALDATFRQMSSFVQDGSSFVKLLALGSVQMCGVGRFPALPPLSPALSDVPYRVNEITKEKEQCCVSMAAGLPHFSAGIFRCWGRDTFIALRGLLLVTGRHLEARNIILAFAGTLRHGLIPNLLGEGVGARFNCRDAVWWWLQCIQDYCTMVPNGTDILQCPVSRMYPTDDSKPLTAGTVDQLLYEVIHEAMERHIQGIEFRESNAGPQIDRNMRDEGFNIEAKVNPDTGFVYGGNRFNCGTWMDKMGESERARNKGIPATPRDGSAVEIVGLCKSTLRWLVELHKKGLFPYNTVKIQRDGKQLSVAYDEWEMKIQKNFEKMFYVSHDPNDPNEKHPELVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTVEHAWKALNMAEEKLLGPLGMKTLDPDDMVYCGVYDNSLDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKIFFAKKMGKDTYDKTVYLVKNVLSRHNVHMERSSWKGLPELTNENGQYCSFSCETQAWSLAAVLEVLHDL

Target ORF information:

RefSeq Version	XM_009298484.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298484.2

ORF Insert Sequence:

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agla ( XM_009298483.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296758.1 Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X3, mRNA.

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CloneID	ODa06903
Clone ID Related Accession (Same CDS sequence)	XM_009298484.3 , XM_009298483.2 , XM_005163618.2 , XM_009298484.2 , XM_009298483.3 , XM_009298485.2 , XM_005163618.3 , XM_009298485.3
Accession Version	XM_009298483.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298483.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296758.1
CDS	165..4766
Translation	MTAKQTRVMVLNDMEKLDRTLFRLDQGFELQFRLGPTLQGKHVRVYTNYPTEGERFDRQKFHALDWINPTAREDDSDKFCTLDLKISGSYQYYFGHGGDEKSGGGYIVVDPVLRVGADNHVLPLDCITIQTYLSKCLGPLDEWSDRLRVAKEAGYNMIHFTPLQTLGESRSCYSLADQLELNPDFSPSGQTYTWTDAGNLVEKLKNEWNMLCITDVVYNHTASNSKWIKKHPECGYNLVNSPHLKPAWVLDRALWHVTCAIADGKYEDRGLPALIQDHPHLHAIRGVLWQEVFPKIKLWEFFQVKIEALVEQFRALLQSGAKPDSSKTDGKKQLKIIQDPQYRRFGNTVDMNTALELFVPNGNSPGAIEDCCNWLRRKLEELNGEQFHEVKHHQEQATNCIDGTVSYERLADHGPKLGPITRKHPLVTRYFTFPFEETTLEQDLELINQPEKSCHFLAHNGWVMGDDPLRNFAEPGSNVYIRRELICWGDSVKLRYGSGPEDCPYLWAHMQKYTEITAKHFVGVRLDNCHSTPLHVAEFMLDAARRLRPDLYVVAELFTGSEELDNVFVTRLGITSLIREAMSAGDSHEEGRLVYRYGGEPVGAFLQPSLRPLVPSIAHAMFLDVTHDNECPIQIRSVYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWSSQAKPSSPNEVNLQTGIIAGKLALNRLHQELAAKGFSQVFVDQVDEDIVAVTRHCPSTHQSVVAVCRTAFKNPKHHRYTDEVPPMCIPGKIEEVVLEARTVERQTGTYKKDERSINGMPEYTVEIKEHIQLADSKVVRQAGVTSRGRSEYVQEIVFEKLTPGSVIAFRVSLDPKSQELVGVLRNHLIQFNPLYKAGSVPDSNTPDILKSPLTQIMSKLTLADMNLLLFRCDSEEKEDGGGCYNIPGWTPLKYGGLQGLLSVMAEIRPKNDLGHPFCDNLRQGDWMIDYVSNRLVSRGGPLAEVGQWFQAMFAYLKHIPRYLIPCYFDAILVGAFTTALDATFRQMSSFVQDGSSFVKLLALGSVQMCGVGRFPALPPLSPALSDVPYRVNEITKEKEQCCVSMAAGLPHFSAGIFRCWGRDTFIALRGLLLVTGRHLEARNIILAFAGTLRHGLIPNLLGEGVGARFNCRDAVWWWLQCIQDYCTMVPNGTDILQCPVSRMYPTDDSKPLTAGTVDQLLYEVIHEAMERHIQGIEFRESNAGPQIDRNMRDEGFNIEAKVNPDTGFVYGGNRFNCGTWMDKMGESERARNKGIPATPRDGSAVEIVGLCKSTLRWLVELHKKGLFPYNTVKIQRDGKQLSVAYDEWEMKIQKNFEKMFYVSHDPNDPNEKHPELVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTVEHAWKALNMAEEKLLGPLGMKTLDPDDMVYCGVYDNSLDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKIFFAKKMGKDTYDKTVYLVKNVLSRHNVHMERSSWKGLPELTNENGQYCSFSCETQAWSLAAVLEVLHDL

Target ORF information:

RefSeq Version	XM_009298483.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298483.3

ORF Insert Sequence:

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agla ( XM_009298485.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296760.1 Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X4, mRNA.

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CloneID	ODa06903
Clone ID Related Accession (Same CDS sequence)	XM_009298484.3 , XM_009298483.2 , XM_005163618.2 , XM_009298484.2 , XM_009298483.3 , XM_009298485.2 , XM_005163618.3 , XM_009298485.3
Accession Version	XM_009298485.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glycogen debranching enzyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296760.1
CDS	115..4716
Translation	MTAKQTRVMVLNDMEKLDRTLFRLDQGFELQFRLGPTLQGKHVRVYTNYPTEGERFDRQKFHALDWINPTAREDDSDKFCTLDLKISGSYQYYFGHGGDEKSGGGYIVVDPVLRVGADNHVLPLDCITIQTYLSKCLGPLDEWSDRLRVAKEAGYNMIHFTPLQTLGESRSCYSLADQLELNPDFSPSGQTYTWTDAGNLVEKLKNEWNMLCITDVVYNHTASNSKWIKKHPECGYNLVNSPHLKPAWVLDRALWHVTCAIADGKYEDRGLPALIQDHPHLHAIRGVLWQEVFPKIKLWEFFQVKIEALVEQFRALLQSGAKPDSSKTDGKKQLKIIQDPQYRRFGNTVDMNTALELFVPNGNSPGAIEDCCNWLRRKLEELNGEQFHEVKHHQEQATNCIDGTVSYERLADHGPKLGPITRKHPLVTRYFTFPFEETTLEQDLELINQPEKSCHFLAHNGWVMGDDPLRNFAEPGSNVYIRRELICWGDSVKLRYGSGPEDCPYLWAHMQKYTEITAKHFVGVRLDNCHSTPLHVAEFMLDAARRLRPDLYVVAELFTGSEELDNVFVTRLGITSLIREAMSAGDSHEEGRLVYRYGGEPVGAFLQPSLRPLVPSIAHAMFLDVTHDNECPIQIRSVYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWSSQAKPSSPNEVNLQTGIIAGKLALNRLHQELAAKGFSQVFVDQVDEDIVAVTRHCPSTHQSVVAVCRTAFKNPKHHRYTDEVPPMCIPGKIEEVVLEARTVERQTGTYKKDERSINGMPEYTVEIKEHIQLADSKVVRQAGVTSRGRSEYVQEIVFEKLTPGSVIAFRVSLDPKSQELVGVLRNHLIQFNPLYKAGSVPDSNTPDILKSPLTQIMSKLTLADMNLLLFRCDSEEKEDGGGCYNIPGWTPLKYGGLQGLLSVMAEIRPKNDLGHPFCDNLRQGDWMIDYVSNRLVSRGGPLAEVGQWFQAMFAYLKHIPRYLIPCYFDAILVGAFTTALDATFRQMSSFVQDGSSFVKLLALGSVQMCGVGRFPALPPLSPALSDVPYRVNEITKEKEQCCVSMAAGLPHFSAGIFRCWGRDTFIALRGLLLVTGRHLEARNIILAFAGTLRHGLIPNLLGEGVGARFNCRDAVWWWLQCIQDYCTMVPNGTDILQCPVSRMYPTDDSKPLTAGTVDQLLYEVIHEAMERHIQGIEFRESNAGPQIDRNMRDEGFNIEAKVNPDTGFVYGGNRFNCGTWMDKMGESERARNKGIPATPRDGSAVEIVGLCKSTLRWLVELHKKGLFPYNTVKIQRDGKQLSVAYDEWEMKIQKNFEKMFYVSHDPNDPNEKHPELVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTVEHAWKALNMAEEKLLGPLGMKTLDPDDMVYCGVYDNSLDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKIFFAKKMGKDTYDKTVYLVKNVLSRHNVHMERSSWKGLPELTNENGQYCSFSCETQAWSLAAVLEVLHDL

Target ORF information:

RefSeq Version	XM_009298485.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298485.2

ORF Insert Sequence:

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agla ( XM_005163618.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163675.1 Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X1, mRNA.

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CloneID	ODa06903
Clone ID Related Accession (Same CDS sequence)	XM_009298484.3 , XM_009298483.2 , XM_005163618.2 , XM_009298484.2 , XM_009298483.3 , XM_009298485.2 , XM_005163618.3 , XM_009298485.3
Accession Version	XM_005163618.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163618.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163675.1
CDS	175..4776
Translation	MTAKQTRVMVLNDMEKLDRTLFRLDQGFELQFRLGPTLQGKHVRVYTNYPTEGERFDRQKFHALDWINPTAREDDSDKFCTLDLKISGSYQYYFGHGGDEKSGGGYIVVDPVLRVGADNHVLPLDCITIQTYLSKCLGPLDEWSDRLRVAKEAGYNMIHFTPLQTLGESRSCYSLADQLELNPDFSPSGQTYTWTDAGNLVEKLKNEWNMLCITDVVYNHTASNSKWIKKHPECGYNLVNSPHLKPAWVLDRALWHVTCAIADGKYEDRGLPALIQDHPHLHAIRGVLWQEVFPKIKLWEFFQVKIEALVEQFRALLQSGAKPDSSKTDGKKQLKIIQDPQYRRFGNTVDMNTALELFVPNGNSPGAIEDCCNWLRRKLEELNGEQFHEVKHHQEQATNCIDGTVSYERLADHGPKLGPITRKHPLVTRYFTFPFEETTLEQDLELINQPEKSCHFLAHNGWVMGDDPLRNFAEPGSNVYIRRELICWGDSVKLRYGSGPEDCPYLWAHMQKYTEITAKHFVGVRLDNCHSTPLHVAEFMLDAARRLRPDLYVVAELFTGSEELDNVFVTRLGITSLIREAMSAGDSHEEGRLVYRYGGEPVGAFLQPSLRPLVPSIAHAMFLDVTHDNECPIQIRSVYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWSSQAKPSSPNEVNLQTGIIAGKLALNRLHQELAAKGFSQVFVDQVDEDIVAVTRHCPSTHQSVVAVCRTAFKNPKHHRYTDEVPPMCIPGKIEEVVLEARTVERQTGTYKKDERSINGMPEYTVEIKEHIQLADSKVVRQAGVTSRGRSEYVQEIVFEKLTPGSVIAFRVSLDPKSQELVGVLRNHLIQFNPLYKAGSVPDSNTPDILKSPLTQIMSKLTLADMNLLLFRCDSEEKEDGGGCYNIPGWTPLKYGGLQGLLSVMAEIRPKNDLGHPFCDNLRQGDWMIDYVSNRLVSRGGPLAEVGQWFQAMFAYLKHIPRYLIPCYFDAILVGAFTTALDATFRQMSSFVQDGSSFVKLLALGSVQMCGVGRFPALPPLSPALSDVPYRVNEITKEKEQCCVSMAAGLPHFSAGIFRCWGRDTFIALRGLLLVTGRHLEARNIILAFAGTLRHGLIPNLLGEGVGARFNCRDAVWWWLQCIQDYCTMVPNGTDILQCPVSRMYPTDDSKPLTAGTVDQLLYEVIHEAMERHIQGIEFRESNAGPQIDRNMRDEGFNIEAKVNPDTGFVYGGNRFNCGTWMDKMGESERARNKGIPATPRDGSAVEIVGLCKSTLRWLVELHKKGLFPYNTVKIQRDGKQLSVAYDEWEMKIQKNFEKMFYVSHDPNDPNEKHPELVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTVEHAWKALNMAEEKLLGPLGMKTLDPDDMVYCGVYDNSLDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKIFFAKKMGKDTYDKTVYLVKNVLSRHNVHMERSSWKGLPELTNENGQYCSFSCETQAWSLAAVLEVLHDL

Target ORF information:

RefSeq Version	XM_005163618.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163618.3

ORF Insert Sequence:

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agla ( XM_009298485.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296760.1 Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X5, mRNA.

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CloneID	ODa06903
Clone ID Related Accession (Same CDS sequence)	XM_009298484.3 , XM_009298483.2 , XM_005163618.2 , XM_009298484.2 , XM_009298483.3 , XM_009298485.2 , XM_005163618.3 , XM_009298485.3
Accession Version	XM_009298485.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296760.1
CDS	114..4715
Translation	MTAKQTRVMVLNDMEKLDRTLFRLDQGFELQFRLGPTLQGKHVRVYTNYPTEGERFDRQKFHALDWINPTAREDDSDKFCTLDLKISGSYQYYFGHGGDEKSGGGYIVVDPVLRVGADNHVLPLDCITIQTYLSKCLGPLDEWSDRLRVAKEAGYNMIHFTPLQTLGESRSCYSLADQLELNPDFSPSGQTYTWTDAGNLVEKLKNEWNMLCITDVVYNHTASNSKWIKKHPECGYNLVNSPHLKPAWVLDRALWHVTCAIADGKYEDRGLPALIQDHPHLHAIRGVLWQEVFPKIKLWEFFQVKIEALVEQFRALLQSGAKPDSSKTDGKKQLKIIQDPQYRRFGNTVDMNTALELFVPNGNSPGAIEDCCNWLRRKLEELNGEQFHEVKHHQEQATNCIDGTVSYERLADHGPKLGPITRKHPLVTRYFTFPFEETTLEQDLELINQPEKSCHFLAHNGWVMGDDPLRNFAEPGSNVYIRRELICWGDSVKLRYGSGPEDCPYLWAHMQKYTEITAKHFVGVRLDNCHSTPLHVAEFMLDAARRLRPDLYVVAELFTGSEELDNVFVTRLGITSLIREAMSAGDSHEEGRLVYRYGGEPVGAFLQPSLRPLVPSIAHAMFLDVTHDNECPIQIRSVYDSLPSSAIVSMACCATGSTRGYDELVPHQISVVSEERFYTKWSSQAKPSSPNEVNLQTGIIAGKLALNRLHQELAAKGFSQVFVDQVDEDIVAVTRHCPSTHQSVVAVCRTAFKNPKHHRYTDEVPPMCIPGKIEEVVLEARTVERQTGTYKKDERSINGMPEYTVEIKEHIQLADSKVVRQAGVTSRGRSEYVQEIVFEKLTPGSVIAFRVSLDPKSQELVGVLRNHLIQFNPLYKAGSVPDSNTPDILKSPLTQIMSKLTLADMNLLLFRCDSEEKEDGGGCYNIPGWTPLKYGGLQGLLSVMAEIRPKNDLGHPFCDNLRQGDWMIDYVSNRLVSRGGPLAEVGQWFQAMFAYLKHIPRYLIPCYFDAILVGAFTTALDATFRQMSSFVQDGSSFVKLLALGSVQMCGVGRFPALPPLSPALSDVPYRVNEITKEKEQCCVSMAAGLPHFSAGIFRCWGRDTFIALRGLLLVTGRHLEARNIILAFAGTLRHGLIPNLLGEGVGARFNCRDAVWWWLQCIQDYCTMVPNGTDILQCPVSRMYPTDDSKPLTAGTVDQLLYEVIHEAMERHIQGIEFRESNAGPQIDRNMRDEGFNIEAKVNPDTGFVYGGNRFNCGTWMDKMGESERARNKGIPATPRDGSAVEIVGLCKSTLRWLVELHKKGLFPYNTVKIQRDGKQLSVAYDEWEMKIQKNFEKMFYVSHDPNDPNEKHPELVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTVEHAWKALNMAEEKLLGPLGMKTLDPDDMVYCGVYDNSLDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKIFFAKKMGKDTYDKTVYLVKNVLSRHNVHMERSSWKGLPELTNENGQYCSFSCETQAWSLAAVLEVLHDL

Target ORF information:

RefSeq Version	XM_009298485.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a (agla), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298485.3

ORF Insert Sequence:

ATGACAGCTA AACAAACAAG AGTGATGGTC CTCAATGACA TGGAGAAACT CGACAGGACT 
CTCTTCCGTC TGGACCAAGG GTTTGAGCTC CAATTTCGTC TGGGTCCCAC TCTTCAAGGG 
AAACATGTAC GTGTTTACAC CAACTACCCC ACAGAAGGCG AGCGCTTTGA TCGCCAAAAG 
TTTCATGCAT TAGACTGGAT TAACCCTACT GCAAGAGAAG ATGATTCAGA CAAGTTCTGC 
ACACTTGACC TCAAGATTTC AGGCTCGTAC CAGTATTATT TTGGCCACGG TGGTGATGAG 
AAGTCTGGTG GTGGATATAT TGTGGTGGAC CCAGTGCTCA GGGTAGGAGC AGATAACCAT 
GTCTTGCCCC TGGACTGCAT CACTATTCAG ACATACCTTT CCAAATGCCT CGGGCCCCTG 
GATGAGTGGT CAGACAGGCT AAGGGTCGCC AAAGAGGCAG GGTACAACAT GATTCACTTT 
ACACCACTGC AGACGCTGGG TGAGTCTCGC TCCTGTTACT CTCTGGCAGA TCAGCTGGAG 
CTCAACCCAG ACTTCTCTCC TTCGGGACAA ACGTACACCT GGACAGATGC TGGCAACCTG 
GTGGAGAAGC TAAAGAATGA GTGGAATATG CTTTGCATCA CGGATGTGGT TTATAATCAT 
ACAGCTTCTA ACAGCAAATG GATTAAGAAG CATCCAGAGT GTGGTTATAA CCTGGTAAAC 
TCTCCTCATC TGAAACCGGC CTGGGTGCTG GACAGAGCCC TGTGGCACGT CACCTGTGCT 
ATAGCTGATG GCAAATATGA AGATCGTGGT CTGCCAGCTC TCATCCAGGA TCACCCACAC 
CTCCATGCCA TTCGAGGAGT TCTCTGGCAG GAGGTGTTTC CCAAGATCAA ACTGTGGGAA 
TTCTTCCAAG TTAAAATAGA GGCGCTGGTG GAGCAGTTCA GAGCTTTACT TCAGAGCGGA 
GCCAAACCAG ACAGCAGCAA GACTGATGGC AAAAAGCAGC TGAAGATCAT TCAAGACCCT 
CAGTACAGAC GCTTTGGTAA CACAGTGGAC ATGAACACGG CTCTAGAACT CTTTGTGCCC 
AATGGGAACA GTCCTGGTGC CATTGAAGAC TGCTGTAATT GGCTAAGGAG GAAACTGGAG 
GAACTCAATG GGGAGCAGTT TCATGAAGTA AAGCACCACC AGGAGCAGGC TACTAATTGC 
ATTGATGGAA CAGTAAGCTA TGAGAGACTG GCTGACCATG GACCCAAACT GGGCCCCATC 
ACCAGGAAAC ATCCACTGGT TACCAGATAT TTCACTTTCC CATTCGAGGA GACAACTTTG 
GAGCAAGATC TGGAGCTGAT TAATCAACCA GAGAAGTCCT GTCACTTCCT GGCCCACAAC 
GGGTGGGTCA TGGGGGACGA TCCCTTGAGG AACTTCGCTG AGCCAGGCTC CAACGTCTAC 
ATCAGGAGGG AGCTGATCTG CTGGGGCGAC AGCGTTAAAC TGCGCTATGG CAGCGGGCCC 
GAGGACTGCC CCTACCTGTG GGCCCACATG CAAAAATACA CCGAAATCAC TGCCAAGCAC 
TTTGTGGGAG TGCGTCTGGA CAACTGCCAT TCAACTCCAC TGCATGTGGC TGAGTTCATG 
CTGGATGCCG CCCGTAGACT CAGGCCTGAC CTGTATGTGG TGGCTGAGCT CTTCACTGGC 
AGCGAGGAGC TGGATAATGT CTTTGTGACT AGATTAGGAA TCACTTCACT TATCAGAGAG 
GCGATGAGTG CAGGAGACAG TCATGAGGAG GGCAGGCTGG TCTATCGGTA TGGAGGAGAA 
CCAGTGGGAG CTTTTCTTCA GCCCAGTCTC AGGCCTCTGG TGCCCAGCAT TGCCCACGCA 
ATGTTTCTGG ACGTCACACA TGACAACGAG TGCCCCATTC AGATCCGCTC TGTGTATGAT 
TCGCTGCCCA GCTCTGCTAT TGTTTCCATG GCCTGCTGTG CCACTGGCAG CACCAGAGGA 
TATGATGAGT TGGTGCCACA CCAGATCTCA GTGGTATCTG AGGAGCGTTT CTATACCAAG 
TGGAGTTCTC AAGCCAAGCC CTCGTCTCCT AATGAGGTCA ACCTGCAGAC AGGAATCATT 
GCTGGAAAGC TGGCTCTCAA TCGGCTACAC CAGGAGCTGG CAGCTAAAGG CTTCAGCCAG 
GTGTTTGTGG ACCAGGTGGA TGAGGACATT GTTGCAGTGA CTCGTCATTG TCCCAGCACA 
CACCAGTCAG TTGTGGCTGT GTGTCGCACT GCGTTCAAGA ACCCCAAACA CCATCGCTAT 
ACAGACGAAG TTCCACCAAT GTGCATTCCA GGTAAGATAG AGGAGGTGGT GCTGGAGGCG 
CGGACCGTGG AGAGGCAGAC GGGCACCTAC AAAAAAGATG AGAGGAGCAT TAATGGCATG 
CCTGAGTACA CGGTGGAGAT TAAAGAGCAC ATCCAGCTGG CAGACAGTAA GGTGGTCCGT 
CAGGCTGGAG TGACGTCTCG AGGTCGCAGC GAATACGTGC AGGAGATTGT GTTTGAGAAG 
CTGACACCTG GAAGCGTTAT TGCCTTCAGG GTGAGTCTGG ACCCCAAATC CCAGGAGCTG 
GTTGGTGTTT TGAGAAACCA TCTCATTCAG TTTAATCCTC TGTATAAAGC AGGAAGCGTG 
CCTGATTCAA ACACACCTGA CATACTGAAG AGTCCGCTGA CACAGATTAT GTCTAAGTTG 
ACTCTGGCCG ATATGAATCT TCTGCTGTTT CGTTGTGATT CAGAGGAAAA AGAAGATGGA 
GGCGGCTGCT ACAACATCCC CGGCTGGACA CCACTTAAAT ACGGTGGTTT ACAAGGTCTG 
TTGTCAGTGA TGGCAGAGAT TCGACCCAAA AATGATCTTG GCCACCCGTT CTGTGACAAC 
CTAAGGCAAG GGGATTGGAT GATAGATTAT GTGAGCAATA GATTGGTGTC TCGCGGGGGC 
CCGCTGGCTG AGGTGGGTCA GTGGTTTCAG GCTATGTTTG CTTACTTGAA GCACATTCCC 
CGCTACCTCA TTCCCTGTTA CTTTGATGCC ATCCTGGTTG GGGCCTTCAC CACTGCCCTT 
GATGCCACTT TCAGACAGAT GTCAAGTTTT GTGCAAGATG GCTCATCTTT TGTCAAGTTG 
CTGGCTCTGG GATCAGTCCA GATGTGTGGT GTTGGACGTT TCCCAGCTCT TCCACCTCTC 
TCGCCAGCCC TAAGCGACGT GCCTTACCGG GTCAATGAAA TCACTAAGGA AAAGGAGCAG 
TGCTGTGTCT CTATGGCGGC AGGCCTGCCG CATTTCTCCG CCGGAATTTT CCGTTGTTGG 
GGCAGGGATA CCTTCATTGC CCTGCGAGGA CTACTACTGG TCACCGGCCG TCACCTTGAG 
GCCAGGAACA TCATCTTGGC CTTTGCTGGC ACATTGAGAC ATGGCTTGAT CCCCAACCTG 
CTGGGTGAGG GGGTAGGGGC CCGCTTTAAT TGTCGTGATG CAGTGTGGTG GTGGCTACAG 
TGCATTCAGG ACTACTGTAC CATGGTGCCC AATGGTACTG ACATCCTTCA GTGCCCTGTG 
TCCCGAATGT ACCCCACTGA TGACTCGAAG CCCCTGACAG CTGGCACTGT GGATCAGCTA 
CTGTATGAGG TTATTCATGA GGCCATGGAG CGCCACATAC AGGGTATAGA GTTCAGAGAG 
AGCAATGCCG GACCACAGAT TGATCGTAAC ATGAGAGACG AAGGTTTCAA TATAGAGGCC 
AAGGTGAACC CTGACACTGG CTTTGTGTAT GGTGGAAATC GGTTCAACTG TGGGACCTGG 
ATGGACAAAA TGGGAGAGAG TGAGAGGGCC CGCAACAAAG GGATACCGGC TACACCCAGA 
GATGGATCAG CGGTGGAAAT TGTGGGTCTG TGCAAATCTA CCTTACGCTG GCTGGTGGAG 
CTCCATAAAA AAGGGCTCTT TCCTTATAAT ACTGTGAAAA TACAGAGAGA CGGGAAACAG 
CTCTCTGTGG CCTATGATGA ATGGGAAATG AAAATCCAGA AGAACTTTGA GAAGATGTTC 
TATGTGTCTC ATGACCCGAA TGACCCTAAC GAGAAGCATC CTGAGCTGGT CCATAAGCGT 
GGCATTTACA AAGACAGCTA TGGAGCCTCC AGCCCCTGGT GTGATTACCA GCTCAGACCC 
AACTTTACCA TAGCCATGGT GGTGGCTCCA GAGCTGTTTA CTGTGGAGCA TGCCTGGAAA 
GCGCTGAACA TGGCAGAAGA GAAGCTGCTC GGACCTCTTG GGATGAAGAC TTTAGACCCA 
GATGACATGG TTTACTGTGG CGTTTATGAC AATTCGCTGG ACAATGACAA CTACAACCTC 
GCCAAAGGCT TCAACTATCA TCAGGGCCCT GAGTGGCTGT GGCCTATTGG ATACTTTCTC 
CGCGCAAAGA TCTTCTTTGC AAAGAAAATG GGCAAAGACA CTTATGACAA AACTGTGTAT 
TTGGTGAAGA ATGTTCTCTC TCGCCATAAT GTCCACATGG AAAGATCATC CTGGAAGGGT 
CTTCCGGAGC TCACTAATGA GAACGGGCAG TACTGCTCCT TCAGCTGTGA AACTCAGGCC 
TGGTCTCTCG CCGCAGTGCT CGAGGTGCTT CATGATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	agla
Entrez Gene ID	567798
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a
Synonyms	agl,si:ch211-153l6.1
General protein information	Preferred Names amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase a Names glycogen debranching enzyme amylo-1, 6-glucosidase, 4-alpha-glucanotransferase a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Mus musculus (house mouse)	Agl	NP_001074795.1
Bos taurus (cattle)	AGL	NP_001179400.1
Homo sapiens (human)	AGL	NP_000635.2
	AGL	XP_001106287.1
Canis lupus familiaris (dog)	AGL	NP_001041561.1
Rattus norvegicus (Norway rat)	Agl	NP_001102034.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001200	XP_321957.3
Caenorhabditis elegans (roundworm)	agl-1	NP_496984.1
Drosophila melanogaster (fruit fly)	CG9485	NP_726062.2
Magnaporthe oryzae (rice blast fungus)	MGG_00063	XP_003719078.1
Xenopus tropicalis (tropical clawed frog)	agl	NP_001072609.1
Pan troglodytes (chimpanzee)	AGL	XP_001159352.1
Danio rerio (zebrafish)	agla	NP_001166124.1
Saccharomyces cerevisiae (baker's yeast)	GDB1	NP_015510.1
Eremothecium gossypii	AGOS_AEL276C	NP_984584.1
Neurospora crassa	NCU00743	XP_964396.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

agla cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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