myo5aa gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021473333.1	XP_021329008.1	unconventional myosin-Va isoform X6
XM_009293798.2	XP_009292073.1	unconventional myosin-Va isoform X5
XM_009293794.2	XP_009292069.1	unconventional myosin-Va isoform X1
XM_009293795.2	XP_009292070.1	unconventional myosin-Va isoform X2
XM_009293797.2	XP_009292072.1	unconventional myosin-Va isoform X4
XM_009293795.3	XP_009292070.1	unconventional myosin-Va isoform X2
XM_009293797.3	XP_009292072.1	unconventional myosin-Va isoform X4
XM_021467867.1	XP_021323542.1	unconventional myosin-Va isoform X9
XM_021473328.1	XP_021329003.1	unconventional myosin-Va isoform X1
XM_021473338.1	XP_021329013.1	unconventional myosin-Va isoform X11
XM_017352118.2	XP_017207607.1	unconventional myosin-Va isoform X11
XM_005173676.3	XP_005173733.1	unconventional myosin-Va isoform X8
XM_021473329.1	XP_021329004.1	unconventional myosin-Va isoform X2
XM_021473332.1	XP_021329007.1	unconventional myosin-Va isoform X5
XM_021473334.1	XP_021329009.1	unconventional myosin-Va isoform X7
XM_021473335.1	XP_021329010.1	unconventional myosin-Va isoform X8
XM_021473336.1	XP_021329011.1	unconventional myosin-Va isoform X9
XM_009293794.3	XP_009292069.1	unconventional myosin-Va isoform X1
XM_009293798.3	XP_009292073.1	unconventional myosin-Va isoform X5
XM_017352118.1	XP_017207607.1	unconventional myosin-Va isoform X10
XM_021473337.1	XP_021329012.1	unconventional myosin-Va isoform X10
XM_005173675.4	XP_005173732.1	unconventional myosin-Va isoform X6
XM_005173676.4	XP_005173733.1	unconventional myosin-Va isoform X7
XM_009293796.2	XP_009292071.1	unconventional myosin-Va isoform X3
XM_009293799.2	XP_009292074.1	unconventional myosin-Va isoform X6
XM_005173675.3	XP_005173732.1	unconventional myosin-Va isoform X7
XM_009293800.2	XP_009292075.1	unconventional myosin-Va isoform X9
XM_009293796.3	XP_009292071.1	unconventional myosin-Va isoform X3
XM_009293800.3	XP_009292075.1	unconventional myosin-Va isoform X8
XM_021467868.1	XP_021323543.1	unconventional myosin-Va isoform X10
XM_021473330.1	XP_021329005.1	unconventional myosin-Va isoform X3
XM_021473331.1	XP_021329006.1	unconventional myosin-Va isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa146059	XM_021473336.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa146060	XM_021473337.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa149977	XM_021467867.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa149978	XM_021467868.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa146051	XM_021473328.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa146052	XM_021473329.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa146053	XM_021473330.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa146054	XM_021473331.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa146055	XM_021473332.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa146056	XM_021473333.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa146057	XM_021473334.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa146058	XM_021473335.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa146061	XM_021473338.1 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa55977	XM_017352118.2 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa23511	XM_005173675.4 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa23512	XM_005173676.4 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43730	XM_009293794.3 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43731	XM_009293795.3 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43732	XM_009293796.3 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43733	XM_009293797.3 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43734	XM_009293798.3 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43735	XM_009293799.2 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43736	XM_009293800.3 Latest version!	Danio rerio myosin VAa (myo5aa), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa43731	XM_009293795.2	Danio rerio myosin VAa (myo5aa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43733	XM_009293797.2	Danio rerio myosin VAa (myo5aa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43730	XM_009293794.2	Danio rerio myosin VAa (myo5aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43734	XM_009293798.2	Danio rerio myosin VAa (myo5aa), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa55977	XM_017352118.1	Danio rerio myosin VAa (myo5aa), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa23512	XM_005173676.3	Danio rerio myosin VAa (myo5aa), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa23511	XM_005173675.3	Danio rerio myosin VAa (myo5aa), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43732	XM_009293796.2	Danio rerio myosin VAa (myo5aa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa43736	XM_009293800.2	Danio rerio myosin VAa (myo5aa), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

myo5aa ( XM_021473336.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329011.1 Danio rerio myosin VAa (myo5aa), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa146059
Clone ID Related Accession (Same CDS sequence)	XM_021473336.1
Accession Version	XM_021473336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395043.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGT CTGAATTATA TACAAAGTAT GCACGGGTCT GGATCCCGGA TGACGAGGAG 
GTTTGGAGAT CAGCAGAGCT CACTAAAGAC TACAGACAAG GAGACGGAGT ACTGCAACTA 
CAGCTGGAGG ATGGAAAGGA CCTGGAGTTT AAATTGGACC CCAAGACGAA CAACTTGCCA 
CACCTGCGTA ACCCGGATAT CCTGGTGGGA GAAAATGACC TGACTGCTCT CAGCTACCTT 
CACGAACCTG CTGTGTTACA CAACCTGAAA GTACGCTTCA TCGACTCCAA GCTCATCTAC 
ACATACTGCG GTATTGTTCT AGTCGCTATA AACCCTTATG AGACTCTTCC CATCTATGGA 
GCGGACATCA TTAACGCATA CAGTGGGCAG AACATGGGCG ACATGGACCC TCACATCTTC 
GCAGTGGCTG AAGAAGCGTA TAAGCAGATG GCCAGGGATG AGAGGAATCA GTCTATTATT 
GTAAGTGGAG AATCTGGAGC GGGAAAAACG GTTTCGGCCA AATACGCCAT GCGTTACTTT 
GCCACAGTCA GTGGATCTGC CAGCGAAGCC AACGTGGAGG AGAAAGTTCT GGCATCCAAT 
CCCATCATGG AGTCTATTGG TAACGCCAAG ACCACCAGGA ATGACAACAG CAGTCGATTT 
GGGAAATACA TTGAGATTGG CTTTGATAAG AAGTATCACA TCATTGGTGC GAACATGAGG 
ACATATCTGC TGGAGAAATC ACGTGTGGTG TTTCAGGCGG ATGAAGAGAG GAATTATCAT 
ATCTTCTATC AGCTCTGTGC ATCGGCTCAC CTCCCGGAGT TTAAAGCCCT GAAACTCGGG 
AAGGCTAATG ATTTCCACTA CACCAAACAG GGCAGGAATC CAGTCATAGA TGGAGTAGAT 
GATGCCAAGG AGATGTCTAC CACCAGGAAC GCCTTCATAC TGTTAGGTAT AAATGAGTCC 
TATCAGATGG GCTTGTTTCA GATATTGGCT TCCATTTTGC ACCTTGGAAA TGTAGACGTG 
AAGGATAGAG ACTCGGACAG CAGTATTATT CCACCAAACA ACGGTCACCT GAGTGTGTTT 
TGTGAGTTGA TGGGAGTGAC ATATCAAGAC ATGTCTCATT GGCTCTGTCA TAAGAAGTTG 
AAGACTGCGA CTGAGACATA CATAAAGCCC ATACCCAAAC TACAGGCTAT AAACGCCCGC 
GACGCTCTCG CCAAACATAT CTACGCCAAA CTCTTCAACT GGATTGTGGA CCACGTGAAC 
AAAGCTCTGC ATTCAACGGT CAAGCAACAC TCTTTCATCG GAGTCTTGGA CATTTACGGG 
TTCGAGACTT TTGAAATCAA CAGTTTTGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTACAGCAAC AGTTCAACAT GCATGTGTTT AAGTTGGAGC AGGAGGAGTA TATGAAAGAG 
CAGATCCCCT GGACTCTCAT TGATTTCTAT GATAATCAGC CGTGTATCAA CCTCATAGAG 
GCCAAGATGG GCATCCTGGA CCTGCTGGAT GAAGAGTGCA AGATGCCGAA GGGTTCGGAT 
GACTCCTGGG CTCAGAAACT CTATAACACT CATTTGAAGA CGTGTGCACT TTTTGAAAAG 
CCGCGGATGT CCAATAAAGC TTTCATTATT CAGCACTTTG CAGATAAGGT GGAGTATCAA 
TGTGATGGTT TTCTGGAGAA GAATAAAGAC ACGGTGAATG AGGAGCAGAT AAATGTTTTG 
AAGGCCAGTA AGAAGTTTGA CCTGCTAGTG GAGCTGTTCC ATGATGAGGA GAAAGCCACA 
AGTCCCACCG GTGCTGCCCC TGGCCCTGGA GGACGCACTC GCCTCAGTGT CAAACCAGAC 
AAGGGCAAAT CCAGCCAAGC CAGCAAAGAA CACAAGAAGA CAGTCGGACT GCAGTTCCGG 
AACTCTCTGC AGCTGCTGAT GGAGACACTG AATGCCACTA CACCTCATTA TGTTCGATGC 
ATTAAACCCA ACGATTACAA ACATGCCTTC ACGTTTGACC CCAAGAGGGC AGTGCAGCAA 
CTGCGTGCTT GTGGAGTTTT GGAAACAATC CGCATCTCTG CAGCTGGTTT CCCATCGAGG 
TGGACGTATC AAGAGTTCTT TAGCAGGTAT CGTGTACTGA TGAAACAAAA AGATGTTCTG 
ACGGACAAGA AGATGACCTG TAAGAATGTT CTGGAGAAAC TGGTGCAGGA TCCAGATAAG 
TACCAGTTTG GAAAGACCAA GATTTTTTTC CGCGCAGGTC AGGTTGCATA CCTGGAGAAA 
CTGCGTGCTG ATAAGCTGCG CGCTGCCTGT ATCCGCATCC AGAAGACCAT CCGCTGCTGG 
CTGGCACGCA AGAAGTACCT GCGTATGAAG CATGCTGCCA CGACTATCCA GAGATTCGTC 
CGTGGATACC AGGCCCGCTG TCTTGCCAAA TTCCTGCGTC GCACACGTGC TGCGATCATT 
ATTCAGAAGT ACCAGCGCAT GTACATCCAG AAAACGTGTT ACAAACGCAA GCAGGCTGCC 
GCGCTGGCCA TGCAGTGCAT CCTCAGAGCG TATATGGCCA GACAGCTTTA CAAAGCGCTG 
CTGCGTGAGC ACAAGGCTGT CATTATTCAG AAGATGGTGC GTGGCTGGCT GGCTCGGCAG 
TGGTTCAAGC GCTCGCTCAA AGCCATCGTT TACCTGCAGT GCTGCATTCG GCGAATGCGA 
GCCAAACGAG AACTCAAGAA GCTCAAGATC GAAGCACGCT CGGTCGAACA CTTCAAGAAG 
CTCAACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAGGATAGA CGATCAGAAC 
AAGGAGAACC GTTCCATGAG CGAACGTTTG AACACTCTAG AGACCAGCCA TGCTGTTGAG 
TCAGAGCGTA TGCGCGCCGA AGTGACGCGT CTGCGGGGAG CAGAGGAGGA CGCCAAAAAC 
AACGCCAACA AAGTCACATC TCTTCTGGAG GAGCTGGAAC GACTGCGCAA AGACCTGCAG 
AACACACAGA AGGAGAAGAA AGCCATTGAG GACTGGGCTC AGACCTACCA GGACGAGATG 
GAAAAGATGA TCTCAGAGCT AAAAGAGCAA AACCAGCTGC TGAAGACAGA GAAAAACAAC 
CTAAACCAAC TGATCCAGGA ACAGAGCCAG CAGTGGACAG ACAAAATGCA GAGAGCTCTG 
AAAGAGGAAA CCCAGCAGTT AGAAAACGAT TTAAACGAGG AGCGATCTCG CTACCAAAAT 
CTCCTCACCG AGCACCTCCG ACTAGAGGAG AAATACGACG ATTTGAAAGA AGAGATCACA 
CTCGCTGTGA ATGTTCCAAA ACCTGGTCAC CGGCGCACAG ACTCCACACA CAGCAGCAAC 
GAATCAGAAT GTACCTACAA CTCAGAGTTC GCAGAGTCAG AGGAGGGATC CCGCGTCGGG 
GAGGATGTTT CCAAGTCAAT CCTGGACATG TCTCTGTTCC TGAAGCTGCA GAAGAGAGTT 
TCCGAACTCG AGCAAGAAAA GCAGTCGCTG CAGAACGAAC TGGATCGCAG AGAAGAGCAG 
TTTCAGCGGG CTAGAGCTAG GGATGATGAA GAACATAAGA AAGCTCGTGG TGCAGAGCTG 
GAGTATGAAT CTCTCAAGCG TCAGGAGCTG GAGTCTGAGA ACAAGAAGCT GAAGCATGAT 
CTGAATCAGA TGCGTCAGTC TCTCAGAGGC AGCGTTGAGG CCGGTTCTGG TGCTCCTGGT 
TCTCCTGCGT ACACCGTTCT TCTGGATCAG CTCAATGCCT CCAACGAGGA ACTGGAAGTG 
CGCAAAGAGG AGGTGCTCAT CCTGCGCTCA CAGCTCGTCA GTCAGAAAGA AGCCATGCAG 
CACAAGACGA CTATGACTGA ACCTCTGCCA TACATTGAGG ATGTGCAGAA GCTCACTGAT 
GCCAAGGAGA TCTCACAGGC CTACATGGGC CTCAAAGACA CCAACAGATC CCCCAGGTTC 
ACACGCAGGC CGACTGAAGT GAACGAGCTC ATGAGCAGGT TGAGATCCCC ATTCCCTGAC 
TACCATAAGC TGAATGAGGA TGGAGAACTG TGGCTGGTTT ATGAAGGGTT AAAGGAAACT 
AACAGGTTAA AGAACAGAGA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329011.1
CDS	221..4327
Translation	MAASELYTKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRSPRFTRRPTEVNELMSRLRSPFPDYHKLNEDGELWLVYEGLKETNRLKNRER

Target ORF information:

RefSeq Version	XM_021473336.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473336.1

ORF Insert Sequence:

ATGGCTGCGT CTGAATTATA TACAAAGTAT GCACGGGTCT GGATCCCGGA TGACGAGGAG 
GTTTGGAGAT CAGCAGAGCT CACTAAAGAC TACAGACAAG GAGACGGAGT ACTGCAACTA 
CAGCTGGAGG ATGGAAAGGA CCTGGAGTTT AAATTGGACC CCAAGACGAA CAACTTGCCA 
CACCTGCGTA ACCCGGATAT CCTGGTGGGA GAAAATGACC TGACTGCTCT CAGCTACCTT 
CACGAACCTG CTGTGTTACA CAACCTGAAA GTACGCTTCA TCGACTCCAA GCTCATCTAC 
ACATACTGCG GTATTGTTCT AGTCGCTATA AACCCTTATG AGACTCTTCC CATCTATGGA 
GCGGACATCA TTAACGCATA CAGTGGGCAG AACATGGGCG ACATGGACCC TCACATCTTC 
GCAGTGGCTG AAGAAGCGTA TAAGCAGATG GCCAGGGATG AGAGGAATCA GTCTATTATT 
GTAAGTGGAG AATCTGGAGC GGGAAAAACG GTTTCGGCCA AATACGCCAT GCGTTACTTT 
GCCACAGTCA GTGGATCTGC CAGCGAAGCC AACGTGGAGG AGAAAGTTCT GGCATCCAAT 
CCCATCATGG AGTCTATTGG TAACGCCAAG ACCACCAGGA ATGACAACAG CAGTCGATTT 
GGGAAATACA TTGAGATTGG CTTTGATAAG AAGTATCACA TCATTGGTGC GAACATGAGG 
ACATATCTGC TGGAGAAATC ACGTGTGGTG TTTCAGGCGG ATGAAGAGAG GAATTATCAT 
ATCTTCTATC AGCTCTGTGC ATCGGCTCAC CTCCCGGAGT TTAAAGCCCT GAAACTCGGG 
AAGGCTAATG ATTTCCACTA CACCAAACAG GGCAGGAATC CAGTCATAGA TGGAGTAGAT 
GATGCCAAGG AGATGTCTAC CACCAGGAAC GCCTTCATAC TGTTAGGTAT AAATGAGTCC 
TATCAGATGG GCTTGTTTCA GATATTGGCT TCCATTTTGC ACCTTGGAAA TGTAGACGTG 
AAGGATAGAG ACTCGGACAG CAGTATTATT CCACCAAACA ACGGTCACCT GAGTGTGTTT 
TGTGAGTTGA TGGGAGTGAC ATATCAAGAC ATGTCTCATT GGCTCTGTCA TAAGAAGTTG 
AAGACTGCGA CTGAGACATA CATAAAGCCC ATACCCAAAC TACAGGCTAT AAACGCCCGC 
GACGCTCTCG CCAAACATAT CTACGCCAAA CTCTTCAACT GGATTGTGGA CCACGTGAAC 
AAAGCTCTGC ATTCAACGGT CAAGCAACAC TCTTTCATCG GAGTCTTGGA CATTTACGGG 
TTCGAGACTT TTGAAATCAA CAGTTTTGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTACAGCAAC AGTTCAACAT GCATGTGTTT AAGTTGGAGC AGGAGGAGTA TATGAAAGAG 
CAGATCCCCT GGACTCTCAT TGATTTCTAT GATAATCAGC CGTGTATCAA CCTCATAGAG 
GCCAAGATGG GCATCCTGGA CCTGCTGGAT GAAGAGTGCA AGATGCCGAA GGGTTCGGAT 
GACTCCTGGG CTCAGAAACT CTATAACACT CATTTGAAGA CGTGTGCACT TTTTGAAAAG 
CCGCGGATGT CCAATAAAGC TTTCATTATT CAGCACTTTG CAGATAAGGT GGAGTATCAA 
TGTGATGGTT TTCTGGAGAA GAATAAAGAC ACGGTGAATG AGGAGCAGAT AAATGTTTTG 
AAGGCCAGTA AGAAGTTTGA CCTGCTAGTG GAGCTGTTCC ATGATGAGGA GAAAGCCACA 
AGTCCCACCG GTGCTGCCCC TGGCCCTGGA GGACGCACTC GCCTCAGTGT CAAACCAGAC 
AAGGGCAAAT CCAGCCAAGC CAGCAAAGAA CACAAGAAGA CAGTCGGACT GCAGTTCCGG 
AACTCTCTGC AGCTGCTGAT GGAGACACTG AATGCCACTA CACCTCATTA TGTTCGATGC 
ATTAAACCCA ACGATTACAA ACATGCCTTC ACGTTTGACC CCAAGAGGGC AGTGCAGCAA 
CTGCGTGCTT GTGGAGTTTT GGAAACAATC CGCATCTCTG CAGCTGGTTT CCCATCGAGG 
TGGACGTATC AAGAGTTCTT TAGCAGGTAT CGTGTACTGA TGAAACAAAA AGATGTTCTG 
ACGGACAAGA AGATGACCTG TAAGAATGTT CTGGAGAAAC TGGTGCAGGA TCCAGATAAG 
TACCAGTTTG GAAAGACCAA GATTTTTTTC CGCGCAGGTC AGGTTGCATA CCTGGAGAAA 
CTGCGTGCTG ATAAGCTGCG CGCTGCCTGT ATCCGCATCC AGAAGACCAT CCGCTGCTGG 
CTGGCACGCA AGAAGTACCT GCGTATGAAG CATGCTGCCA CGACTATCCA GAGATTCGTC 
CGTGGATACC AGGCCCGCTG TCTTGCCAAA TTCCTGCGTC GCACACGTGC TGCGATCATT 
ATTCAGAAGT ACCAGCGCAT GTACATCCAG AAAACGTGTT ACAAACGCAA GCAGGCTGCC 
GCGCTGGCCA TGCAGTGCAT CCTCAGAGCG TATATGGCCA GACAGCTTTA CAAAGCGCTG 
CTGCGTGAGC ACAAGGCTGT CATTATTCAG AAGATGGTGC GTGGCTGGCT GGCTCGGCAG 
TGGTTCAAGC GCTCGCTCAA AGCCATCGTT TACCTGCAGT GCTGCATTCG GCGAATGCGA 
GCCAAACGAG AACTCAAGAA GCTCAAGATC GAAGCACGCT CGGTCGAACA CTTCAAGAAG 
CTCAACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAGGATAGA CGATCAGAAC 
AAGGAGAACC GTTCCATGAG CGAACGTTTG AACACTCTAG AGACCAGCCA TGCTGTTGAG 
TCAGAGCGTA TGCGCGCCGA AGTGACGCGT CTGCGGGGAG CAGAGGAGGA CGCCAAAAAC 
AACGCCAACA AAGTCACATC TCTTCTGGAG GAGCTGGAAC GACTGCGCAA AGACCTGCAG 
AACACACAGA AGGAGAAGAA AGCCATTGAG GACTGGGCTC AGACCTACCA GGACGAGATG 
GAAAAGATGA TCTCAGAGCT AAAAGAGCAA AACCAGCTGC TGAAGACAGA GAAAAACAAC 
CTAAACCAAC TGATCCAGGA ACAGAGCCAG CAGTGGACAG ACAAAATGCA GAGAGCTCTG 
AAAGAGGAAA CCCAGCAGTT AGAAAACGAT TTAAACGAGG AGCGATCTCG CTACCAAAAT 
CTCCTCACCG AGCACCTCCG ACTAGAGGAG AAATACGACG ATTTGAAAGA AGAGATCACA 
CTCGCTGTGA ATGTTCCAAA ACCTGGTCAC CGGCGCACAG ACTCCACACA CAGCAGCAAC 
GAATCAGAAT GTACCTACAA CTCAGAGTTC GCAGAGTCAG AGGAGGGATC CCGCGTCGGG 
GAGGATGTTT CCAAGTCAAT CCTGGACATG TCTCTGTTCC TGAAGCTGCA GAAGAGAGTT 
TCCGAACTCG AGCAAGAAAA GCAGTCGCTG CAGAACGAAC TGGATCGCAG AGAAGAGCAG 
TTTCAGCGGG CTAGAGCTAG GGATGATGAA GAACATAAGA AAGCTCGTGG TGCAGAGCTG 
GAGTATGAAT CTCTCAAGCG TCAGGAGCTG GAGTCTGAGA ACAAGAAGCT GAAGCATGAT 
CTGAATCAGA TGCGTCAGTC TCTCAGAGGC AGCGTTGAGG CCGGTTCTGG TGCTCCTGGT 
TCTCCTGCGT ACACCGTTCT TCTGGATCAG CTCAATGCCT CCAACGAGGA ACTGGAAGTG 
CGCAAAGAGG AGGTGCTCAT CCTGCGCTCA CAGCTCGTCA GTCAGAAAGA AGCCATGCAG 
CACAAGACGA CTATGACTGA ACCTCTGCCA TACATTGAGG ATGTGCAGAA GCTCACTGAT 
GCCAAGGAGA TCTCACAGGC CTACATGGGC CTCAAAGACA CCAACAGATC CCCCAGGTTC 
ACACGCAGGC CGACTGAAGT GAACGAGCTC ATGAGCAGGT TGAGATCCCC ATTCCCTGAC 
TACCATAAGC TGAATGAGGA TGGAGAACTG TGGCTGGTTT ATGAAGGGTT AAAGGAAACT 
AACAGGTTAA AGAACAGAGA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_021473337.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329012.1 Danio rerio myosin VAa (myo5aa), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa146060
Clone ID Related Accession (Same CDS sequence)	XM_021473337.1
Accession Version	XM_021473337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGATCCCCCA GGTTCACACG CAGGCCGACT GAAGTGAACG AGCTCATGAG CAGGTTGAGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329012.1
CDS	102..4124
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRSPRFTRRPTEVNELMSRLR

Target ORF information:

RefSeq Version	XM_021473337.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473337.1

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGATCCCCCA GGTTCACACG CAGGCCGACT GAAGTGAACG AGCTCATGAG CAGGTTGAGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_021467867.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323542.1 Danio rerio myosin VAa (myo5aa), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa149977
Clone ID Related Accession (Same CDS sequence)	XM_021467867.1
Accession Version	XM_021467867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGT CTGAATTATA TACAAAGTAT GCACGGGTCT GGATCCCGGA TGACGAGGAG 
GTTTGGAGAT CAGCAGAGCT CACTAAAGAC TACAGACAAG GAGACGGAGT ACTGCAACTA 
CAGCTGGAGG ATGGAAAGGA CCTGGAGTTT AAATTGGACC CCAAGACGAA CAACTTGCCA 
CACCTGCGTA ACCCGGATAT CCTGGTGGGA GAAAATGACC TGACTGCTCT CAGCTACCTT 
CACGAACCTG CTGTGTTACA CAACCTGAAA GTACGCTTCA TCGACTCCAA GCTCATCTAC 
ACATACTGCG GTATTGTTCT AGTCGCTATA AACCCTTATG AGACTCTTCC CATCTATGGA 
GCGGACATCA TTAACGCATA CAGTGGGCAG AACATGGGCG ACATGGACCC TCACATCTTC 
GCAGTGGCTG AAGAAGCGTA TAAGCAGATG GCCAGGGATG AGAGGAATCA GTCTATTATT 
GTAAGTGGAG AATCTGGAGC GGGAAAAACG GTTTCGGCCA AATACGCCAT GCGTTACTTT 
GCCACAGTCA GTGGATCTGC CAGCGAAGCC AACGTGGAGG AGAAAGTTCT GGCATCCAAT 
CCCATAATGG AGTCTATTGG TAACGCCAAG ACCACCAGGA ATGACAACAG CAGTCGATTT 
GGGAAATACA TTGAGATTGG CTTTGATAAG AAGTATCACA TCATTGGTGC GAACATGAGG 
ACATATCTGC TGGAGAAATC ACGTGTGGTG TTTCAGGCGG ATGAAGAGAG GAATTATCAT 
ATCTTCTATC AGCTCTGTGC ATCGGCTCAC CTCCCGGAGT TTAAAGCCCT GAAACTCGGA 
AAGGCTAATG ATTTCCACTA CACAAAACAG GGCAGGAATC CAGTCATAGA TGGAGTAGAT 
GATGCCAAGG AGATGTCTAC CACCAGGAAC GCCTTCATAC TGCTAGGTAT AAATGAGTCC 
TATCAGATGG GCTTGTTTCA GATATTGGCT TCCATTTTGC ACCTTGGAAA TGTAGACGTG 
AAGGATAGAG ACTCGGACAG CAGTATTATT CCACCAAACA ACGGTCACCT GAGTGTGTTT 
TGTGAGTTGA TGGGAGTAAC ATATCAAGAC ATGTCTCATT GGCTCTGTCA TAAGAAGTTG 
AAGACTGCGA CTGAGACATA CATAAAGCCC ATACCCAAAC TACAGGCTAT AAACGCCCGC 
GACGCTCTCG CCAAACATAT CTACGCCAAA CTCTTCAACT GGATTGTAGA CCACGTGAAC 
AAAGCTCTGC ATTCAACGGT CAAGCAACAC TCTTTCATCG GAGTCTTGGA CATTTACGGG 
TTCGAGACTT TTGAAATCAA CAGTTTTGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTACAGCAAC AGTTCAACAT GCATGTGTTT AAGTTGGAGC AGGAGGAGTA TATGAAAGAG 
CAGATCCCGT GGACTCTCAT TGATTTCTAT GATAATCAGC CATGTATCAA CCTCATAGAG 
GCCAAGATGG GCATCCTGGA CCTGCTGGAT GAAGAGTGCA AGATGCCGAA GGGTTCGGAT 
GACTCCTGGG CTCAGAAACT CTATAACACT CATTTGAAGA CGTGTGCACT TTTTGAAAAG 
CCGCGGATGT CCAATAAAGC TTTCATTATT CAGCACTTTG CAGATAAGGT GGAGTATCAA 
TGTGATGGTT TTCTGGAGAA GAATAAAGAC ACGGTGAATG AGGAGCAGAT AAATGTTTTG 
AAGGCCAGTA AGAAGTTTGA CCTGCTAGTG GAGCTGTTCC ATGATGAGGA GAAAGCCACA 
AGTCCCACCG GTGCTGCCCC TGGCCCTGGA GGACGCACTC GCCTCAGTGT CAAACCAGAC 
AAGGGCAAAT CCAGCCAAGC CAGCAAAGAA CACAAGAAGA CAGTCGGACT GCAGTTCCGG 
AACTCTCTGC AGCTGCTGAT GGAGACACTG AATGCCACTA CACCTCATTA TGTTCGATGC 
ATTAAACCCA ACGATTACAA ACATGCCTTC ACGTTTGACC CCAAGAGGGC AGTGCAGCAA 
CTGCGTGCTT GTGGAGTTTT GGAAACAATC CGCATCTCTG CAGCTGGTTT CCCATCGAGG 
TGGACGTATC AAGAGTTCTT TAGCAGGTAT CGTGTACTGA TGAAACAAAA AGATGTTCTG 
ACGGACAAGA AGATGACCTG TAAGAATGTT CTGGAGAAAC TGGTGCAGGA TCCAGATAAG 
TACCAGTTTG GAAAGACCAA GATTTTTTTC CGCGCAGGTC AGGTTGCATA CCTGGAGAAA 
CTGCGTGCTG ATAAGCTGCG CGCTGCCTGT ATCCGCATCC AGAAGACCAT CCGCTGCTGG 
CTGGCACGCA AGAAGTACCT GCGTATGAAG CATGCTGCCA CGACTATCCA GAGATTCGTC 
CGTGGATACC AGGCCCGCTG TCTTGCCAAA TTCCTGCGTC GCACACGTGC TGCGATCATT 
ATTCAGAAGT ACCAGCGCAT GTACATCCAG AAAACGTGTT ACAAACGCAA GCAGGCTGCC 
GCGCTGGCCA TGCAGTGCAT CCTCAGAGCG TATATGGCCA GACAGCTTTA CAAAGCGCTG 
CTGCGTGAGC ACAAGGCTGT CATTATTCAG AAGATGGTGC GTGGCTGGCT GGCTCGGCAG 
TGGTTCAAGC GCTCGCTCAA AGCCATCGTT TACCTGCAGT GCTGCATTCG GCGAATGCGA 
GCCAAACGAG AACTCAAGAA GCTCAAGATC GAAGCACGCT CGGTCGAACA CTTCAAGAAG 
CTCAACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAGGATAGA CGATCAGAAC 
AAGGAGAACC GTTCCATGAG CGAACGTTTG AACACTCTAG AGACCAGCCA TGCTGTTGAG 
TCAGAGCGTA TGCGCGCCGA AGTGACGCGT CTGCGGGGAG CAGAGGAGGA CGCCAAAAAC 
AACGCCAACA AAGTCACATC TCTTCTGGAG GAGCTGGAAC GACTGCGCAA AGACCTGCAG 
AACACACAGA AGGAGAAGAA AGCCATTGAG GACTGGGCTC AGACCTACCA GGACGAGATG 
GAAAAGATGA TCTCAGAGCT AAAAGAGCAA AACCAGCTGC TGAAGACAGA GAAAAACAAC 
CTAAACCAAC TGATCCAGGA ACAGAGCCAG CAGTGGACAG ACAAAATGCA GAGAGCTCTG 
AAAGAGGAAA CCCAGCAGTT AGAAAACGAT TTAAACGAGG AGCGATCTCG CTACCAAAAT 
CTCCTCACCG AGCACCTCCG ACTAGAGGAG AAATACGACG ATTTGAAAGA AGAGATCACA 
CTCGCTGTGA ATGTTCCAAA ACCTGGTCAC CGGCGCACAG ACTCCACACA CAGCAGCAAC 
GAATCAGAAT GTACCTACAA CTCAGAGTTC GCAGAGTCAG AGGAGGGATC CCGCGTCGGG 
GAGGATGTTT CCAAGTCAAT CCTGGACATG TCTCTGTTCC TGAAGCTGCA GAAGAGAGTT 
TCCGAACTCG AGCAAGAAAA GCAGTCGCTG CAGAACGAAC TGGATCGCAG AGAAGAGCAG 
TTTCAGCGGG CTAGAGCTAG GGATGATGAA GAACATAAGA AAGCTCGTGG TGCAGAGCTG 
GAGTATGAAT CTCTCAAGCG TCAGGAGCTG GAGTCTGAGA ACAAGAAGCT GAAGCATGAT 
CTGAATCAGA TGCGTCAGTC TCTCAGAGGC AGCGTTGAGG CCGGTTCTGG TGCTCCTGGT 
TCTCCTGCGT ACACCGTTCT TCTGGATCAG CTCAATGCCT CCAACGAGGA ACTGGAAGTG 
CGCAAAGAGG AGGTGCTCAT CCTGCGCTCA CAGCTCGTCA GTCAGAAAGA AGCCATGCAG 
CACAAGACGA CTATGACTGA ACCTCTGCCA TACATTGAGG ATGTGCAGAA GCTCACTGAT 
GCCAAGGAGA TCTCACAGGC CTACATGGGC CTCAAAGACA CCAACAGATC CCCCAGGTTC 
ACACGCAGGC CGACTGAAGT GAACGAGCTC ATGAGCAGGT TGAGATCCCC ATTCCCTGAC 
TACCATAAGC TGAATGAGGA TGGAGAACTG TGGCTGGTTT ATGAAGGGTT AAAGGAAACT 
AACAGGTTAA AGAACAGAGA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323542.1
CDS	252..4358
Translation	MAASELYTKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRSPRFTRRPTEVNELMSRLRSPFPDYHKLNEDGELWLVYEGLKETNRLKNRER

Target ORF information:

RefSeq Version	XM_021467867.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467867.1

ORF Insert Sequence:

ATGGCTGCGT CTGAATTATA TACAAAGTAT GCACGGGTCT GGATCCCGGA TGACGAGGAG 
GTTTGGAGAT CAGCAGAGCT CACTAAAGAC TACAGACAAG GAGACGGAGT ACTGCAACTA 
CAGCTGGAGG ATGGAAAGGA CCTGGAGTTT AAATTGGACC CCAAGACGAA CAACTTGCCA 
CACCTGCGTA ACCCGGATAT CCTGGTGGGA GAAAATGACC TGACTGCTCT CAGCTACCTT 
CACGAACCTG CTGTGTTACA CAACCTGAAA GTACGCTTCA TCGACTCCAA GCTCATCTAC 
ACATACTGCG GTATTGTTCT AGTCGCTATA AACCCTTATG AGACTCTTCC CATCTATGGA 
GCGGACATCA TTAACGCATA CAGTGGGCAG AACATGGGCG ACATGGACCC TCACATCTTC 
GCAGTGGCTG AAGAAGCGTA TAAGCAGATG GCCAGGGATG AGAGGAATCA GTCTATTATT 
GTAAGTGGAG AATCTGGAGC GGGAAAAACG GTTTCGGCCA AATACGCCAT GCGTTACTTT 
GCCACAGTCA GTGGATCTGC CAGCGAAGCC AACGTGGAGG AGAAAGTTCT GGCATCCAAT 
CCCATAATGG AGTCTATTGG TAACGCCAAG ACCACCAGGA ATGACAACAG CAGTCGATTT 
GGGAAATACA TTGAGATTGG CTTTGATAAG AAGTATCACA TCATTGGTGC GAACATGAGG 
ACATATCTGC TGGAGAAATC ACGTGTGGTG TTTCAGGCGG ATGAAGAGAG GAATTATCAT 
ATCTTCTATC AGCTCTGTGC ATCGGCTCAC CTCCCGGAGT TTAAAGCCCT GAAACTCGGA 
AAGGCTAATG ATTTCCACTA CACAAAACAG GGCAGGAATC CAGTCATAGA TGGAGTAGAT 
GATGCCAAGG AGATGTCTAC CACCAGGAAC GCCTTCATAC TGCTAGGTAT AAATGAGTCC 
TATCAGATGG GCTTGTTTCA GATATTGGCT TCCATTTTGC ACCTTGGAAA TGTAGACGTG 
AAGGATAGAG ACTCGGACAG CAGTATTATT CCACCAAACA ACGGTCACCT GAGTGTGTTT 
TGTGAGTTGA TGGGAGTAAC ATATCAAGAC ATGTCTCATT GGCTCTGTCA TAAGAAGTTG 
AAGACTGCGA CTGAGACATA CATAAAGCCC ATACCCAAAC TACAGGCTAT AAACGCCCGC 
GACGCTCTCG CCAAACATAT CTACGCCAAA CTCTTCAACT GGATTGTAGA CCACGTGAAC 
AAAGCTCTGC ATTCAACGGT CAAGCAACAC TCTTTCATCG GAGTCTTGGA CATTTACGGG 
TTCGAGACTT TTGAAATCAA CAGTTTTGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTACAGCAAC AGTTCAACAT GCATGTGTTT AAGTTGGAGC AGGAGGAGTA TATGAAAGAG 
CAGATCCCGT GGACTCTCAT TGATTTCTAT GATAATCAGC CATGTATCAA CCTCATAGAG 
GCCAAGATGG GCATCCTGGA CCTGCTGGAT GAAGAGTGCA AGATGCCGAA GGGTTCGGAT 
GACTCCTGGG CTCAGAAACT CTATAACACT CATTTGAAGA CGTGTGCACT TTTTGAAAAG 
CCGCGGATGT CCAATAAAGC TTTCATTATT CAGCACTTTG CAGATAAGGT GGAGTATCAA 
TGTGATGGTT TTCTGGAGAA GAATAAAGAC ACGGTGAATG AGGAGCAGAT AAATGTTTTG 
AAGGCCAGTA AGAAGTTTGA CCTGCTAGTG GAGCTGTTCC ATGATGAGGA GAAAGCCACA 
AGTCCCACCG GTGCTGCCCC TGGCCCTGGA GGACGCACTC GCCTCAGTGT CAAACCAGAC 
AAGGGCAAAT CCAGCCAAGC CAGCAAAGAA CACAAGAAGA CAGTCGGACT GCAGTTCCGG 
AACTCTCTGC AGCTGCTGAT GGAGACACTG AATGCCACTA CACCTCATTA TGTTCGATGC 
ATTAAACCCA ACGATTACAA ACATGCCTTC ACGTTTGACC CCAAGAGGGC AGTGCAGCAA 
CTGCGTGCTT GTGGAGTTTT GGAAACAATC CGCATCTCTG CAGCTGGTTT CCCATCGAGG 
TGGACGTATC AAGAGTTCTT TAGCAGGTAT CGTGTACTGA TGAAACAAAA AGATGTTCTG 
ACGGACAAGA AGATGACCTG TAAGAATGTT CTGGAGAAAC TGGTGCAGGA TCCAGATAAG 
TACCAGTTTG GAAAGACCAA GATTTTTTTC CGCGCAGGTC AGGTTGCATA CCTGGAGAAA 
CTGCGTGCTG ATAAGCTGCG CGCTGCCTGT ATCCGCATCC AGAAGACCAT CCGCTGCTGG 
CTGGCACGCA AGAAGTACCT GCGTATGAAG CATGCTGCCA CGACTATCCA GAGATTCGTC 
CGTGGATACC AGGCCCGCTG TCTTGCCAAA TTCCTGCGTC GCACACGTGC TGCGATCATT 
ATTCAGAAGT ACCAGCGCAT GTACATCCAG AAAACGTGTT ACAAACGCAA GCAGGCTGCC 
GCGCTGGCCA TGCAGTGCAT CCTCAGAGCG TATATGGCCA GACAGCTTTA CAAAGCGCTG 
CTGCGTGAGC ACAAGGCTGT CATTATTCAG AAGATGGTGC GTGGCTGGCT GGCTCGGCAG 
TGGTTCAAGC GCTCGCTCAA AGCCATCGTT TACCTGCAGT GCTGCATTCG GCGAATGCGA 
GCCAAACGAG AACTCAAGAA GCTCAAGATC GAAGCACGCT CGGTCGAACA CTTCAAGAAG 
CTCAACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAGGATAGA CGATCAGAAC 
AAGGAGAACC GTTCCATGAG CGAACGTTTG AACACTCTAG AGACCAGCCA TGCTGTTGAG 
TCAGAGCGTA TGCGCGCCGA AGTGACGCGT CTGCGGGGAG CAGAGGAGGA CGCCAAAAAC 
AACGCCAACA AAGTCACATC TCTTCTGGAG GAGCTGGAAC GACTGCGCAA AGACCTGCAG 
AACACACAGA AGGAGAAGAA AGCCATTGAG GACTGGGCTC AGACCTACCA GGACGAGATG 
GAAAAGATGA TCTCAGAGCT AAAAGAGCAA AACCAGCTGC TGAAGACAGA GAAAAACAAC 
CTAAACCAAC TGATCCAGGA ACAGAGCCAG CAGTGGACAG ACAAAATGCA GAGAGCTCTG 
AAAGAGGAAA CCCAGCAGTT AGAAAACGAT TTAAACGAGG AGCGATCTCG CTACCAAAAT 
CTCCTCACCG AGCACCTCCG ACTAGAGGAG AAATACGACG ATTTGAAAGA AGAGATCACA 
CTCGCTGTGA ATGTTCCAAA ACCTGGTCAC CGGCGCACAG ACTCCACACA CAGCAGCAAC 
GAATCAGAAT GTACCTACAA CTCAGAGTTC GCAGAGTCAG AGGAGGGATC CCGCGTCGGG 
GAGGATGTTT CCAAGTCAAT CCTGGACATG TCTCTGTTCC TGAAGCTGCA GAAGAGAGTT 
TCCGAACTCG AGCAAGAAAA GCAGTCGCTG CAGAACGAAC TGGATCGCAG AGAAGAGCAG 
TTTCAGCGGG CTAGAGCTAG GGATGATGAA GAACATAAGA AAGCTCGTGG TGCAGAGCTG 
GAGTATGAAT CTCTCAAGCG TCAGGAGCTG GAGTCTGAGA ACAAGAAGCT GAAGCATGAT 
CTGAATCAGA TGCGTCAGTC TCTCAGAGGC AGCGTTGAGG CCGGTTCTGG TGCTCCTGGT 
TCTCCTGCGT ACACCGTTCT TCTGGATCAG CTCAATGCCT CCAACGAGGA ACTGGAAGTG 
CGCAAAGAGG AGGTGCTCAT CCTGCGCTCA CAGCTCGTCA GTCAGAAAGA AGCCATGCAG 
CACAAGACGA CTATGACTGA ACCTCTGCCA TACATTGAGG ATGTGCAGAA GCTCACTGAT 
GCCAAGGAGA TCTCACAGGC CTACATGGGC CTCAAAGACA CCAACAGATC CCCCAGGTTC 
ACACGCAGGC CGACTGAAGT GAACGAGCTC ATGAGCAGGT TGAGATCCCC ATTCCCTGAC 
TACCATAAGC TGAATGAGGA TGGAGAACTG TGGCTGGTTT ATGAAGGGTT AAAGGAAACT 
AACAGGTTAA AGAACAGAGA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_021467868.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323543.1 Danio rerio myosin VAa (myo5aa), transcript variant X10, mRNA.

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CloneID	ODa149978
Clone ID Related Accession (Same CDS sequence)	XM_021467868.1
Accession Version	XM_021467868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGATCCCCCA GGTTCACACG CAGGCCGACT GAAGTGAACG AGCTCATGAG CAGGTTGAGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323543.1
CDS	170..4192
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRSPRFTRRPTEVNELMSRLR

Target ORF information:

RefSeq Version	XM_021467868.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467868.1

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGATCCCCCA GGTTCACACG CAGGCCGACT GAAGTGAACG AGCTCATGAG CAGGTTGAGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_021473328.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329003.1 Danio rerio myosin VAa (myo5aa), transcript variant X1, mRNA.

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CloneID	ODa146051
Clone ID Related Accession (Same CDS sequence)	XM_021473328.1
Accession Version	XM_021473328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395043.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGGCTGTTGG AGTCTCAGTT GCAGGCTCAA CGTCGCTCTC ATGACAACGA GGTGGAAGTT 
TTGCGTGGAG AGCTGCACAG TCTTAAGGAG GAGAACAACC GTCAGCAGCA GCTGTTGGCG 
CAGAACCTGC AACTACCTCC AGAGGCCAGG ATTGAAGCCA GTCTACAGCA CGAGATCACA 
CGCCTCACCA ACGAGAACCT GGAACTTCAC TCTGATGATC CAAAGGAGTA TAAAGCCTAC 
AGAATCAGCA TGTTACGCAG GATGGTTGAT CTTATGGAGC AGTTGGAGAA ACAGGACAAG 
ACCGTTCGTA AGCTGAAGAA ACAGCTCAAA GTATTTGCTA AGAAGATCAA TGATCTGGAA 
GGGGGGCAGA TGGACGTGTC TCCCGGACAA ACGGCTGACG AGCCTGTTCA TCCGGTGAAC 
ATTCCACGCA GAGAGAAAGA TTTCCAGGGC ATGCTGGAAT ACAAGAAAGA GGATGAACTC 
AAACTGGTCA AAAATATCAT ACTTGAGCTG AAGCCTCGTG GTGTTGCAGT GAATCTGATA 
CCAGGTCTGC CCGCATACAT CCTCTTCATG TGTCTGCGCC ATGCTGACTA CATCAACGAT 
GACCAGAAAG TGCGATCTCT ACTCACCTCC GTCATCAACA GCATCAAGAA GATCCTCAAG 
AAACGAGGTG ATGACTTTGA GACTGTTTCC TTCTGGTTGG CCAACACATG TCGTTTTCTG 
CACTGCCTGA AACAGTACAG TGGTGATGAG CAATTCATGA AGCATAATAG CCCAAAGCAG 
AACGAACACT GTCTCTCTAA CTTTGACCTG GCAGAGTACC GGCAGGTTCT GAGCGATCTG 
GCCATTCAGA TCTACCAGCA GCTCATCAAG TGTATGGAAA ACATCCTGCA GCCCATGATC 
GTCTCTGGCA TGCTGGAACA TGAAACCATC CAGGGTGTGT CAGGAGTGAA GCCCACAGGT 
CTCCGCAAGA GGACGTCAAG TATTGCTGAT GAGGGCACGT ACACACTGGA CTCCATCATC 
CGTCAGCTCA ACACCTTCCA CTCCATCATG TGTCACCACG GCACTGACCC CGAGCTCATC 
AAACAGGTGG TCAAGCAGCA GTTCTACATC ATCGGCGCTG TCACCCTCAA CAACCTGCTG 
CTGCGCAAAG ACATGTGCTC CTGGAGCAAG GGCATGCAGA TCAGGTATAA CGTGAGTCAG 
CTGGAGGAAT GGCTGAGGGA TAAGAACCTC ATGACATGCG GCGCTAAAGA AACACTGGAG 
CCGTTGATTC AGGCTGCGCA ACTACTGCAG GTGAAGAAGA AAACTGATGA GGACGCCGAG 
GCCATCTGCT CTATGTGTAA CGCCCTCTCC ACAGCACAGA TTGTGAAAGT GCTGAACTTG 
TACACACCTG TCAATGCATT TGAAGAGCGA GTGTCAGTGT TGTTCATCAG AACAATACAG 
ACTCGTTTAC GGGATCGAAA GGAGTCGCCT CAGCTTCTGA TGGACACCAA ACTCATCTAC 
CCCGTGACCT TCCCCTTTAA CCCCTCCTCC CTTGCCCTGG AAACCATTCA GATCCCCAGC 
AGCCTCAATC TGGCCTTCCT CACTCGTGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329003.1
CDS	100..5652
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_021473328.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473328.1

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGGCTGTTGG AGTCTCAGTT GCAGGCTCAA CGTCGCTCTC ATGACAACGA GGTGGAAGTT 
TTGCGTGGAG AGCTGCACAG TCTTAAGGAG GAGAACAACC GTCAGCAGCA GCTGTTGGCG 
CAGAACCTGC AACTACCTCC AGAGGCCAGG ATTGAAGCCA GTCTACAGCA CGAGATCACA 
CGCCTCACCA ACGAGAACCT GGAACTTCAC TCTGATGATC CAAAGGAGTA TAAAGCCTAC 
AGAATCAGCA TGTTACGCAG GATGGTTGAT CTTATGGAGC AGTTGGAGAA ACAGGACAAG 
ACCGTTCGTA AGCTGAAGAA ACAGCTCAAA GTATTTGCTA AGAAGATCAA TGATCTGGAA 
GGGGGGCAGA TGGACGTGTC TCCCGGACAA ACGGCTGACG AGCCTGTTCA TCCGGTGAAC 
ATTCCACGCA GAGAGAAAGA TTTCCAGGGC ATGCTGGAAT ACAAGAAAGA GGATGAACTC 
AAACTGGTCA AAAATATCAT ACTTGAGCTG AAGCCTCGTG GTGTTGCAGT GAATCTGATA 
CCAGGTCTGC CCGCATACAT CCTCTTCATG TGTCTGCGCC ATGCTGACTA CATCAACGAT 
GACCAGAAAG TGCGATCTCT ACTCACCTCC GTCATCAACA GCATCAAGAA GATCCTCAAG 
AAACGAGGTG ATGACTTTGA GACTGTTTCC TTCTGGTTGG CCAACACATG TCGTTTTCTG 
CACTGCCTGA AACAGTACAG TGGTGATGAG CAATTCATGA AGCATAATAG CCCAAAGCAG 
AACGAACACT GTCTCTCTAA CTTTGACCTG GCAGAGTACC GGCAGGTTCT GAGCGATCTG 
GCCATTCAGA TCTACCAGCA GCTCATCAAG TGTATGGAAA ACATCCTGCA GCCCATGATC 
GTCTCTGGCA TGCTGGAACA TGAAACCATC CAGGGTGTGT CAGGAGTGAA GCCCACAGGT 
CTCCGCAAGA GGACGTCAAG TATTGCTGAT GAGGGCACGT ACACACTGGA CTCCATCATC 
CGTCAGCTCA ACACCTTCCA CTCCATCATG TGTCACCACG GCACTGACCC CGAGCTCATC 
AAACAGGTGG TCAAGCAGCA GTTCTACATC ATCGGCGCTG TCACCCTCAA CAACCTGCTG 
CTGCGCAAAG ACATGTGCTC CTGGAGCAAG GGCATGCAGA TCAGGTATAA CGTGAGTCAG 
CTGGAGGAAT GGCTGAGGGA TAAGAACCTC ATGACATGCG GCGCTAAAGA AACACTGGAG 
CCGTTGATTC AGGCTGCGCA ACTACTGCAG GTGAAGAAGA AAACTGATGA GGACGCCGAG 
GCCATCTGCT CTATGTGTAA CGCCCTCTCC ACAGCACAGA TTGTGAAAGT GCTGAACTTG 
TACACACCTG TCAATGCATT TGAAGAGCGA GTGTCAGTGT TGTTCATCAG AACAATACAG 
ACTCGTTTAC GGGATCGAAA GGAGTCGCCT CAGCTTCTGA TGGACACCAA ACTCATCTAC 
CCCGTGACCT TCCCCTTTAA CCCCTCCTCC CTTGCCCTGG AAACCATTCA GATCCCCAGC 
AGCCTCAATC TGGCCTTCCT CACTCGTGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_021473329.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329004.1 Danio rerio myosin VAa (myo5aa), transcript variant X2, mRNA.

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CloneID	ODa146052
Clone ID Related Accession (Same CDS sequence)	XM_021473329.1
Accession Version	XM_021473329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395043.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGG 
CTGTTGGAGT CTCAGTTGCA GGCTCAACGT CGCTCTCATG ACAACGAGGT GGAAGTTTTG 
CGTGGAGAGC TGCACAGTCT TAAGGAGGAG AACAACCGTC AGCAGCAGCT GTTGGCGCAG 
AACCTGCAAC TACCTCCAGA GGCCAGGATT GAAGCCAGTC TACAGCACGA GATCACACGC 
CTCACCAACG AGAACCTGGA ACTTCACTCT GATGATCCAA AGGAGTATAA AGCCTACAGA 
ATCAGCATGT TACGCAGGAT GGTTGATCTT ATGGAGCAGT TGGAGAAACA GGACAAGACC 
GTTCGTAAGC TGAAGAAACA GCTCAAAGTA TTTGCTAAGA AGATCAATGA TCTGGAAGGG 
GGGCAGATGG ACGTGTCTCC CGGACAAACG GCTGACGAGC CTGTTCATCC GGTGAACATT 
CCACGCAGAG AGAAAGATTT CCAGGGCATG CTGGAATACA AGAAAGAGGA TGAACTCAAA 
CTGGTCAAAA ATATCATACT TGAGCTGAAG CCTCGTGGTG TTGCAGTGAA TCTGATACCA 
GGTCTGCCCG CATACATCCT CTTCATGTGT CTGCGCCATG CTGACTACAT CAACGATGAC 
CAGAAAGTGC GATCTCTACT CACCTCCGTC ATCAACAGCA TCAAGAAGAT CCTCAAGAAA 
CGAGGTGATG ACTTTGAGAC TGTTTCCTTC TGGTTGGCCA ACACATGTCG TTTTCTGCAC 
TGCCTGAAAC AGTACAGTGG TGATGAGCAA TTCATGAAGC ATAATAGCCC AAAGCAGAAC 
GAACACTGTC TCTCTAACTT TGACCTGGCA GAGTACCGGC AGGTTCTGAG CGATCTGGCC 
ATTCAGATCT ACCAGCAGCT CATCAAGTGT ATGGAAAACA TCCTGCAGCC CATGATCGTC 
TCTGGCATGC TGGAACATGA AACCATCCAG GGTGTGTCAG GAGTGAAGCC CACAGGTCTC 
CGCAAGAGGA CGTCAAGTAT TGCTGATGAG GGCACGTACA CACTGGACTC CATCATCCGT 
CAGCTCAACA CCTTCCACTC CATCATGTGT CACCACGGCA CTGACCCCGA GCTCATCAAA 
CAGGTGGTCA AGCAGCAGTT CTACATCATC GGCGCTGTCA CCCTCAACAA CCTGCTGCTG 
CGCAAAGACA TGTGCTCCTG GAGCAAGGGC ATGCAGATCA GGTATAACGT GAGTCAGCTG 
GAGGAATGGC TGAGGGATAA GAACCTCATG ACATGCGGCG CTAAAGAAAC ACTGGAGCCG 
TTGATTCAGG CTGCGCAACT ACTGCAGGTG AAGAAGAAAA CTGATGAGGA CGCCGAGGCC 
ATCTGCTCTA TGTGTAACGC CCTCTCCACA GCACAGATTG TGAAAGTGCT GAACTTGTAC 
ACACCTGTCA ATGCATTTGA AGAGCGAGTG TCAGTGTTGT TCATCAGAAC AATACAGACT 
CGTTTACGGG ATCGAAAGGA GTCGCCTCAG CTTCTGATGG ACACCAAACT CATCTACCCC 
GTGACCTTCC CCTTTAACCC CTCCTCCCTT GCCCTGGAAA CCATTCAGAT CCCCAGCAGC 
CTCAATCTGG CCTTCCTCAC TCGTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329004.1
CDS	100..5649
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_021473329.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473329.1

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGG 
CTGTTGGAGT CTCAGTTGCA GGCTCAACGT CGCTCTCATG ACAACGAGGT GGAAGTTTTG 
CGTGGAGAGC TGCACAGTCT TAAGGAGGAG AACAACCGTC AGCAGCAGCT GTTGGCGCAG 
AACCTGCAAC TACCTCCAGA GGCCAGGATT GAAGCCAGTC TACAGCACGA GATCACACGC 
CTCACCAACG AGAACCTGGA ACTTCACTCT GATGATCCAA AGGAGTATAA AGCCTACAGA 
ATCAGCATGT TACGCAGGAT GGTTGATCTT ATGGAGCAGT TGGAGAAACA GGACAAGACC 
GTTCGTAAGC TGAAGAAACA GCTCAAAGTA TTTGCTAAGA AGATCAATGA TCTGGAAGGG 
GGGCAGATGG ACGTGTCTCC CGGACAAACG GCTGACGAGC CTGTTCATCC GGTGAACATT 
CCACGCAGAG AGAAAGATTT CCAGGGCATG CTGGAATACA AGAAAGAGGA TGAACTCAAA 
CTGGTCAAAA ATATCATACT TGAGCTGAAG CCTCGTGGTG TTGCAGTGAA TCTGATACCA 
GGTCTGCCCG CATACATCCT CTTCATGTGT CTGCGCCATG CTGACTACAT CAACGATGAC 
CAGAAAGTGC GATCTCTACT CACCTCCGTC ATCAACAGCA TCAAGAAGAT CCTCAAGAAA 
CGAGGTGATG ACTTTGAGAC TGTTTCCTTC TGGTTGGCCA ACACATGTCG TTTTCTGCAC 
TGCCTGAAAC AGTACAGTGG TGATGAGCAA TTCATGAAGC ATAATAGCCC AAAGCAGAAC 
GAACACTGTC TCTCTAACTT TGACCTGGCA GAGTACCGGC AGGTTCTGAG CGATCTGGCC 
ATTCAGATCT ACCAGCAGCT CATCAAGTGT ATGGAAAACA TCCTGCAGCC CATGATCGTC 
TCTGGCATGC TGGAACATGA AACCATCCAG GGTGTGTCAG GAGTGAAGCC CACAGGTCTC 
CGCAAGAGGA CGTCAAGTAT TGCTGATGAG GGCACGTACA CACTGGACTC CATCATCCGT 
CAGCTCAACA CCTTCCACTC CATCATGTGT CACCACGGCA CTGACCCCGA GCTCATCAAA 
CAGGTGGTCA AGCAGCAGTT CTACATCATC GGCGCTGTCA CCCTCAACAA CCTGCTGCTG 
CGCAAAGACA TGTGCTCCTG GAGCAAGGGC ATGCAGATCA GGTATAACGT GAGTCAGCTG 
GAGGAATGGC TGAGGGATAA GAACCTCATG ACATGCGGCG CTAAAGAAAC ACTGGAGCCG 
TTGATTCAGG CTGCGCAACT ACTGCAGGTG AAGAAGAAAA CTGATGAGGA CGCCGAGGCC 
ATCTGCTCTA TGTGTAACGC CCTCTCCACA GCACAGATTG TGAAAGTGCT GAACTTGTAC 
ACACCTGTCA ATGCATTTGA AGAGCGAGTG TCAGTGTTGT TCATCAGAAC AATACAGACT 
CGTTTACGGG ATCGAAAGGA GTCGCCTCAG CTTCTGATGG ACACCAAACT CATCTACCCC 
GTGACCTTCC CCTTTAACCC CTCCTCCCTT GCCCTGGAAA CCATTCAGAT CCCCAGCAGC 
CTCAATCTGG CCTTCCTCAC TCGTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_021473330.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329005.1 Danio rerio myosin VAa (myo5aa), transcript variant X3, mRNA.

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CloneID	ODa146053
Clone ID Related Accession (Same CDS sequence)	XM_021473330.1
Accession Version	XM_021473330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395043.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGT CTGAATTATA TACAAAGTAT GCACGGGTCT GGATCCCGGA TGACGAGGAG 
GTTTGGAGAT CAGCAGAGCT CACTAAAGAC TACAGACAAG GAGACGGAGT ACTGCAACTA 
CAGCTGGAGG ATGGAAAGGA CCTGGAGTTT AAATTGGACC CCAAGACGAA CAACTTGCCA 
CACCTGCGTA ACCCGGATAT CCTGGTGGGA GAAAATGACC TGACTGCTCT CAGCTACCTT 
CACGAACCTG CTGTGTTACA CAACCTGAAA GTACGCTTCA TCGACTCCAA GCTCATCTAC 
ACATACTGCG GTATTGTTCT AGTCGCTATA AACCCTTATG AGACTCTTCC CATCTATGGA 
GCGGACATCA TTAACGCATA CAGTGGGCAG AACATGGGCG ACATGGACCC TCACATCTTC 
GCAGTGGCTG AAGAAGCGTA TAAGCAGATG GCCAGGGATG AGAGGAATCA GTCTATTATT 
GTAAGTGGAG AATCTGGAGC GGGAAAAACG GTTTCGGCCA AATACGCCAT GCGTTACTTT 
GCCACAGTCA GTGGATCTGC CAGCGAAGCC AACGTGGAGG AGAAAGTTCT GGCATCCAAT 
CCCATCATGG AGTCTATTGG TAACGCCAAG ACCACCAGGA ATGACAACAG CAGTCGATTT 
GGGAAATACA TTGAGATTGG CTTTGATAAG AAGTATCACA TCATTGGTGC GAACATGAGG 
ACATATCTGC TGGAGAAATC ACGTGTGGTG TTTCAGGCGG ATGAAGAGAG GAATTATCAT 
ATCTTCTATC AGCTCTGTGC ATCGGCTCAC CTCCCGGAGT TTAAAGCCCT GAAACTCGGG 
AAGGCTAATG ATTTCCACTA CACCAAACAG GGCAGGAATC CAGTCATAGA TGGAGTAGAT 
GATGCCAAGG AGATGTCTAC CACCAGGAAC GCCTTCATAC TGTTAGGTAT AAATGAGTCC 
TATCAGATGG GCTTGTTTCA GATATTGGCT TCCATTTTGC ACCTTGGAAA TGTAGACGTG 
AAGGATAGAG ACTCGGACAG CAGTATTATT CCACCAAACA ACGGTCACCT GAGTGTGTTT 
TGTGAGTTGA TGGGAGTGAC ATATCAAGAC ATGTCTCATT GGCTCTGTCA TAAGAAGTTG 
AAGACTGCGA CTGAGACATA CATAAAGCCC ATACCCAAAC TACAGGCTAT AAACGCCCGC 
GACGCTCTCG CCAAACATAT CTACGCCAAA CTCTTCAACT GGATTGTGGA CCACGTGAAC 
AAAGCTCTGC ATTCAACGGT CAAGCAACAC TCTTTCATCG GAGTCTTGGA CATTTACGGG 
TTCGAGACTT TTGAAATCAA CAGTTTTGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTACAGCAAC AGTTCAACAT GCATGTGTTT AAGTTGGAGC AGGAGGAGTA TATGAAAGAG 
CAGATCCCCT GGACTCTCAT TGATTTCTAT GATAATCAGC CGTGTATCAA CCTCATAGAG 
GCCAAGATGG GCATCCTGGA CCTGCTGGAT GAAGAGTGCA AGATGCCGAA GGGTTCGGAT 
GACTCCTGGG CTCAGAAACT CTATAACACT CATTTGAAGA CGTGTGCACT TTTTGAAAAG 
CCGCGGATGT CCAATAAAGC TTTCATTATT CAGCACTTTG CAGATAAGGT GGAGTATCAA 
TGTGATGGTT TTCTGGAGAA GAATAAAGAC ACGGTGAATG AGGAGCAGAT AAATGTTTTG 
AAGGCCAGTA AGAAGTTTGA CCTGCTAGTG GAGCTGTTCC ATGATGAGGA GAAAGCCACA 
AGTCCCACCG GTGCTGCCCC TGGCCCTGGA GGACGCACTC GCCTCAGTGT CAAACCAGAC 
AAGGGCAAAT CCAGCCAAGC CAGCAAAGAA CACAAGAAGA CAGTCGGACT GCAGTTCCGG 
AACTCTCTGC AGCTGCTGAT GGAGACACTG AATGCCACTA CACCTCATTA TGTTCGATGC 
ATTAAACCCA ACGATTACAA ACATGCCTTC ACGTTTGACC CCAAGAGGGC AGTGCAGCAA 
CTGCGTGCTT GTGGAGTTTT GGAAACAATC CGCATCTCTG CAGCTGGTTT CCCATCGAGG 
TGGACGTATC AAGAGTTCTT TAGCAGGTAT CGTGTACTGA TGAAACAAAA AGATGTTCTG 
ACGGACAAGA AGATGACCTG TAAGAATGTT CTGGAGAAAC TGGTGCAGGA TCCAGATAAG 
TACCAGTTTG GAAAGACCAA GATTTTTTTC CGCGCAGGTC AGGTTGCATA CCTGGAGAAA 
CTGCGTGCTG ATAAGCTGCG CGCTGCCTGT ATCCGCATCC AGAAGACCAT CCGCTGCTGG 
CTGGCACGCA AGAAGTACCT GCGTATGAAG CATGCTGCCA CGACTATCCA GAGATTCGTC 
CGTGGATACC AGGCCCGCTG TCTTGCCAAA TTCCTGCGTC GCACACGTGC TGCGATCATT 
ATTCAGAAGT ACCAGCGCAT GTACATCCAG AAAACGTGTT ACAAACGCAA GCAGGCTGCC 
GCGCTGGCCA TGCAGTGCAT CCTCAGAGCG TATATGGCCA GACAGCTTTA CAAAGCGCTG 
CTGCGTGAGC ACAAGGCTGT CATTATTCAG AAGATGGTGC GTGGCTGGCT GGCTCGGCAG 
TGGTTCAAGC GCTCGCTCAA AGCCATCGTT TACCTGCAGT GCTGCATTCG GCGAATGCGA 
GCCAAACGAG AACTCAAGAA GCTCAAGATC GAAGCACGCT CGGTCGAACA CTTCAAGAAG 
CTCAACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAGGATAGA CGATCAGAAC 
AAGGAGAACC GTTCCATGAG CGAACGTTTG AACACTCTAG AGACCAGCCA TGCTGTTGAG 
TCAGAGCGTA TGCGCGCCGA AGTGACGCGT CTGCGGGGAG CAGAGGAGGA CGCCAAAAAC 
AACGCCAACA AAGTCACATC TCTTCTGGAG GAGCTGGAAC GACTGCGCAA AGACCTGCAG 
AACACACAGA AGGAGAAGAA AGCCATTGAG GACTGGGCTC AGACCTACCA GGACGAGATG 
GAAAAGATGA TCTCAGAGCT AAAAGAGCAA AACCAGCTGC TGAAGACAGA GAAAAACAAC 
CTAAACCAAC TGATCCAGGA ACAGAGCCAG CAGTGGACAG ACAAAATGCA GAGAGCTCTG 
AAAGAGGAAA CCCAGCAGTT AGAAAACGAT TTAAACGAGG AGCGATCTCG CTACCAAAAT 
CTCCTCACCG AGCACCTCCG ACTAGAGGAG AAATACGACG ATTTGAAAGA AGAGATCACA 
CTCGCTGTGA ATGTTCCAAA ACCTGGTCAC CGGCGCACAG ACTCCACACA CAGCAGCAAC 
GAATCAGAAT GTACCTACAA CTCAGAGTTC GCAGAGTCAG AGGAGGGATC CCGCGTCGGG 
GAGGATGTTT CCAAGTCAAT CCTGGACATG TCTCTGTTCC TGAAGCTGCA GAAGAGAGTT 
TCCGAACTCG AGCAAGAAAA GCAGTCGCTG CAGAACGAAC TGGATCGCAG AGAAGAGCAG 
TTTCAGCGGG CTAGAGCTAG GGATGATGAA GAACATAAGA AAGCTCGTGG TGCAGAGCTG 
GAGTATGAAT CTCTCAAGCG TCAGGAGCTG GAGTCTGAGA ACAAGAAGCT GAAGCATGAT 
CTGAATCAGA TGCGTCAGTC TCTCAGAGGC AGCGTTGAGG CCGGTTCTGG TGCTCCTGGT 
TCTCCTGCGT ACACCGTTCT TCTGGATCAG CTCAATGCCT CCAACGAGGA ACTGGAAGTG 
CGCAAAGAGG AGGTGCTCAT CCTGCGCTCA CAGCTCGTCA GTCAGAAAGA AGCCATGCAG 
CACAAGGATG AGAAGACGAC TATGACTGAA CCTCTGCCAT ACATTGAGGA TGTGCAGAAG 
CTCACTGATG CCAAGGAGAT CTCACAGGCC TACATGGGCC TCAAAGACAC CAACAGGCTG 
TTGGAGTCTC AGTTGCAGGC TCAACGTCGC TCTCATGACA ACGAGGTGGA AGTTTTGCGT 
GGAGAGCTGC ACAGTCTTAA GGAGGAGAAC AACCGTCAGC AGCAGCTGTT GGCGCAGAAC 
CTGCAACTAC CTCCAGAGGC CAGGATTGAA GCCAGTCTAC AGCACGAGAT CACACGCCTC 
ACCAACGAGA ACCTGGAACT TCACTCTGAT GATCCAAAGG AGTATAAAGC CTACAGAATC 
AGCATGTTAC GCAGGATGGT TGATCTTATG GAGCAGTTGG AGAAACAGGA CAAGACCGTT 
CGTAAGCTGA AGAAACAGCT CAAAGTATTT GCTAAGAAGA TCAATGATCT GGAAGGGGGG 
CAGATGGACG TGTCTCCCGG ACAAACGGCT GACGAGCCTG TTCATCCGGT GAACATTCCA 
CGCAGAGAGA AAGATTTCCA GGGCATGCTG GAATACAAGA AAGAGGATGA ACTCAAACTG 
GTCAAAAATA TCATACTTGA GCTGAAGCCT CGTGGTGTTG CAGTGAATCT GATACCAGGT 
CTGCCCGCAT ACATCCTCTT CATGTGTCTG CGCCATGCTG ACTACATCAA CGATGACCAG 
AAAGTGCGAT CTCTACTCAC CTCCGTCATC AACAGCATCA AGAAGATCCT CAAGAAACGA 
GGTGATGACT TTGAGACTGT TTCCTTCTGG TTGGCCAACA CATGTCGTTT TCTGCACTGC 
CTGAAACAGT ACAGTGGTGA TGAGCAATTC ATGAAGCATA ATAGCCCAAA GCAGAACGAA 
CACTGTCTCT CTAACTTTGA CCTGGCAGAG TACCGGCAGG TTCTGAGCGA TCTGGCCATT 
CAGATCTACC AGCAGCTCAT CAAGTGTATG GAAAACATCC TGCAGCCCAT GATCGTCTCT 
GGCATGCTGG AACATGAAAC CATCCAGGGT GTGTCAGGAG TGAAGCCCAC AGGTCTCCGC 
AAGAGGACGT CAAGTATTGC TGATGAGGGC ACGTACACAC TGGACTCCAT CATCCGTCAG 
CTCAACACCT TCCACTCCAT CATGTGTCAC CACGGCACTG ACCCCGAGCT CATCAAACAG 
GTGGTCAAGC AGCAGTTCTA CATCATCGGC GCTGTCACCC TCAACAACCT GCTGCTGCGC 
AAAGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ATAACGTGAG TCAGCTGGAG 
GAATGGCTGA GGGATAAGAA CCTCATGACA TGCGGCGCTA AAGAAACACT GGAGCCGTTG 
ATTCAGGCTG CGCAACTACT GCAGGTGAAG AAGAAAACTG ATGAGGACGC CGAGGCCATC 
TGCTCTATGT GTAACGCCCT CTCCACAGCA CAGATTGTGA AAGTGCTGAA CTTGTACACA 
CCTGTCAATG CATTTGAAGA GCGAGTGTCA GTGTTGTTCA TCAGAACAAT ACAGACTCGT 
TTACGGGATC GAAAGGAGTC GCCTCAGCTT CTGATGGACA CCAAACTCAT CTACCCCGTG 
ACCTTCCCCT TTAACCCCTC CTCCCTTGCC CTGGAAACCA TTCAGATCCC CAGCAGCCTC 
AATCTGGCCT TCCTCACTCG TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329005.1
CDS	218..5764
Translation	MAASELYTKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_021473330.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473330.1

ORF Insert Sequence:

ATGGCTGCGT CTGAATTATA TACAAAGTAT GCACGGGTCT GGATCCCGGA TGACGAGGAG 
GTTTGGAGAT CAGCAGAGCT CACTAAAGAC TACAGACAAG GAGACGGAGT ACTGCAACTA 
CAGCTGGAGG ATGGAAAGGA CCTGGAGTTT AAATTGGACC CCAAGACGAA CAACTTGCCA 
CACCTGCGTA ACCCGGATAT CCTGGTGGGA GAAAATGACC TGACTGCTCT CAGCTACCTT 
CACGAACCTG CTGTGTTACA CAACCTGAAA GTACGCTTCA TCGACTCCAA GCTCATCTAC 
ACATACTGCG GTATTGTTCT AGTCGCTATA AACCCTTATG AGACTCTTCC CATCTATGGA 
GCGGACATCA TTAACGCATA CAGTGGGCAG AACATGGGCG ACATGGACCC TCACATCTTC 
GCAGTGGCTG AAGAAGCGTA TAAGCAGATG GCCAGGGATG AGAGGAATCA GTCTATTATT 
GTAAGTGGAG AATCTGGAGC GGGAAAAACG GTTTCGGCCA AATACGCCAT GCGTTACTTT 
GCCACAGTCA GTGGATCTGC CAGCGAAGCC AACGTGGAGG AGAAAGTTCT GGCATCCAAT 
CCCATCATGG AGTCTATTGG TAACGCCAAG ACCACCAGGA ATGACAACAG CAGTCGATTT 
GGGAAATACA TTGAGATTGG CTTTGATAAG AAGTATCACA TCATTGGTGC GAACATGAGG 
ACATATCTGC TGGAGAAATC ACGTGTGGTG TTTCAGGCGG ATGAAGAGAG GAATTATCAT 
ATCTTCTATC AGCTCTGTGC ATCGGCTCAC CTCCCGGAGT TTAAAGCCCT GAAACTCGGG 
AAGGCTAATG ATTTCCACTA CACCAAACAG GGCAGGAATC CAGTCATAGA TGGAGTAGAT 
GATGCCAAGG AGATGTCTAC CACCAGGAAC GCCTTCATAC TGTTAGGTAT AAATGAGTCC 
TATCAGATGG GCTTGTTTCA GATATTGGCT TCCATTTTGC ACCTTGGAAA TGTAGACGTG 
AAGGATAGAG ACTCGGACAG CAGTATTATT CCACCAAACA ACGGTCACCT GAGTGTGTTT 
TGTGAGTTGA TGGGAGTGAC ATATCAAGAC ATGTCTCATT GGCTCTGTCA TAAGAAGTTG 
AAGACTGCGA CTGAGACATA CATAAAGCCC ATACCCAAAC TACAGGCTAT AAACGCCCGC 
GACGCTCTCG CCAAACATAT CTACGCCAAA CTCTTCAACT GGATTGTGGA CCACGTGAAC 
AAAGCTCTGC ATTCAACGGT CAAGCAACAC TCTTTCATCG GAGTCTTGGA CATTTACGGG 
TTCGAGACTT TTGAAATCAA CAGTTTTGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTACAGCAAC AGTTCAACAT GCATGTGTTT AAGTTGGAGC AGGAGGAGTA TATGAAAGAG 
CAGATCCCCT GGACTCTCAT TGATTTCTAT GATAATCAGC CGTGTATCAA CCTCATAGAG 
GCCAAGATGG GCATCCTGGA CCTGCTGGAT GAAGAGTGCA AGATGCCGAA GGGTTCGGAT 
GACTCCTGGG CTCAGAAACT CTATAACACT CATTTGAAGA CGTGTGCACT TTTTGAAAAG 
CCGCGGATGT CCAATAAAGC TTTCATTATT CAGCACTTTG CAGATAAGGT GGAGTATCAA 
TGTGATGGTT TTCTGGAGAA GAATAAAGAC ACGGTGAATG AGGAGCAGAT AAATGTTTTG 
AAGGCCAGTA AGAAGTTTGA CCTGCTAGTG GAGCTGTTCC ATGATGAGGA GAAAGCCACA 
AGTCCCACCG GTGCTGCCCC TGGCCCTGGA GGACGCACTC GCCTCAGTGT CAAACCAGAC 
AAGGGCAAAT CCAGCCAAGC CAGCAAAGAA CACAAGAAGA CAGTCGGACT GCAGTTCCGG 
AACTCTCTGC AGCTGCTGAT GGAGACACTG AATGCCACTA CACCTCATTA TGTTCGATGC 
ATTAAACCCA ACGATTACAA ACATGCCTTC ACGTTTGACC CCAAGAGGGC AGTGCAGCAA 
CTGCGTGCTT GTGGAGTTTT GGAAACAATC CGCATCTCTG CAGCTGGTTT CCCATCGAGG 
TGGACGTATC AAGAGTTCTT TAGCAGGTAT CGTGTACTGA TGAAACAAAA AGATGTTCTG 
ACGGACAAGA AGATGACCTG TAAGAATGTT CTGGAGAAAC TGGTGCAGGA TCCAGATAAG 
TACCAGTTTG GAAAGACCAA GATTTTTTTC CGCGCAGGTC AGGTTGCATA CCTGGAGAAA 
CTGCGTGCTG ATAAGCTGCG CGCTGCCTGT ATCCGCATCC AGAAGACCAT CCGCTGCTGG 
CTGGCACGCA AGAAGTACCT GCGTATGAAG CATGCTGCCA CGACTATCCA GAGATTCGTC 
CGTGGATACC AGGCCCGCTG TCTTGCCAAA TTCCTGCGTC GCACACGTGC TGCGATCATT 
ATTCAGAAGT ACCAGCGCAT GTACATCCAG AAAACGTGTT ACAAACGCAA GCAGGCTGCC 
GCGCTGGCCA TGCAGTGCAT CCTCAGAGCG TATATGGCCA GACAGCTTTA CAAAGCGCTG 
CTGCGTGAGC ACAAGGCTGT CATTATTCAG AAGATGGTGC GTGGCTGGCT GGCTCGGCAG 
TGGTTCAAGC GCTCGCTCAA AGCCATCGTT TACCTGCAGT GCTGCATTCG GCGAATGCGA 
GCCAAACGAG AACTCAAGAA GCTCAAGATC GAAGCACGCT CGGTCGAACA CTTCAAGAAG 
CTCAACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAGGATAGA CGATCAGAAC 
AAGGAGAACC GTTCCATGAG CGAACGTTTG AACACTCTAG AGACCAGCCA TGCTGTTGAG 
TCAGAGCGTA TGCGCGCCGA AGTGACGCGT CTGCGGGGAG CAGAGGAGGA CGCCAAAAAC 
AACGCCAACA AAGTCACATC TCTTCTGGAG GAGCTGGAAC GACTGCGCAA AGACCTGCAG 
AACACACAGA AGGAGAAGAA AGCCATTGAG GACTGGGCTC AGACCTACCA GGACGAGATG 
GAAAAGATGA TCTCAGAGCT AAAAGAGCAA AACCAGCTGC TGAAGACAGA GAAAAACAAC 
CTAAACCAAC TGATCCAGGA ACAGAGCCAG CAGTGGACAG ACAAAATGCA GAGAGCTCTG 
AAAGAGGAAA CCCAGCAGTT AGAAAACGAT TTAAACGAGG AGCGATCTCG CTACCAAAAT 
CTCCTCACCG AGCACCTCCG ACTAGAGGAG AAATACGACG ATTTGAAAGA AGAGATCACA 
CTCGCTGTGA ATGTTCCAAA ACCTGGTCAC CGGCGCACAG ACTCCACACA CAGCAGCAAC 
GAATCAGAAT GTACCTACAA CTCAGAGTTC GCAGAGTCAG AGGAGGGATC CCGCGTCGGG 
GAGGATGTTT CCAAGTCAAT CCTGGACATG TCTCTGTTCC TGAAGCTGCA GAAGAGAGTT 
TCCGAACTCG AGCAAGAAAA GCAGTCGCTG CAGAACGAAC TGGATCGCAG AGAAGAGCAG 
TTTCAGCGGG CTAGAGCTAG GGATGATGAA GAACATAAGA AAGCTCGTGG TGCAGAGCTG 
GAGTATGAAT CTCTCAAGCG TCAGGAGCTG GAGTCTGAGA ACAAGAAGCT GAAGCATGAT 
CTGAATCAGA TGCGTCAGTC TCTCAGAGGC AGCGTTGAGG CCGGTTCTGG TGCTCCTGGT 
TCTCCTGCGT ACACCGTTCT TCTGGATCAG CTCAATGCCT CCAACGAGGA ACTGGAAGTG 
CGCAAAGAGG AGGTGCTCAT CCTGCGCTCA CAGCTCGTCA GTCAGAAAGA AGCCATGCAG 
CACAAGGATG AGAAGACGAC TATGACTGAA CCTCTGCCAT ACATTGAGGA TGTGCAGAAG 
CTCACTGATG CCAAGGAGAT CTCACAGGCC TACATGGGCC TCAAAGACAC CAACAGGCTG 
TTGGAGTCTC AGTTGCAGGC TCAACGTCGC TCTCATGACA ACGAGGTGGA AGTTTTGCGT 
GGAGAGCTGC ACAGTCTTAA GGAGGAGAAC AACCGTCAGC AGCAGCTGTT GGCGCAGAAC 
CTGCAACTAC CTCCAGAGGC CAGGATTGAA GCCAGTCTAC AGCACGAGAT CACACGCCTC 
ACCAACGAGA ACCTGGAACT TCACTCTGAT GATCCAAAGG AGTATAAAGC CTACAGAATC 
AGCATGTTAC GCAGGATGGT TGATCTTATG GAGCAGTTGG AGAAACAGGA CAAGACCGTT 
CGTAAGCTGA AGAAACAGCT CAAAGTATTT GCTAAGAAGA TCAATGATCT GGAAGGGGGG 
CAGATGGACG TGTCTCCCGG ACAAACGGCT GACGAGCCTG TTCATCCGGT GAACATTCCA 
CGCAGAGAGA AAGATTTCCA GGGCATGCTG GAATACAAGA AAGAGGATGA ACTCAAACTG 
GTCAAAAATA TCATACTTGA GCTGAAGCCT CGTGGTGTTG CAGTGAATCT GATACCAGGT 
CTGCCCGCAT ACATCCTCTT CATGTGTCTG CGCCATGCTG ACTACATCAA CGATGACCAG 
AAAGTGCGAT CTCTACTCAC CTCCGTCATC AACAGCATCA AGAAGATCCT CAAGAAACGA 
GGTGATGACT TTGAGACTGT TTCCTTCTGG TTGGCCAACA CATGTCGTTT TCTGCACTGC 
CTGAAACAGT ACAGTGGTGA TGAGCAATTC ATGAAGCATA ATAGCCCAAA GCAGAACGAA 
CACTGTCTCT CTAACTTTGA CCTGGCAGAG TACCGGCAGG TTCTGAGCGA TCTGGCCATT 
CAGATCTACC AGCAGCTCAT CAAGTGTATG GAAAACATCC TGCAGCCCAT GATCGTCTCT 
GGCATGCTGG AACATGAAAC CATCCAGGGT GTGTCAGGAG TGAAGCCCAC AGGTCTCCGC 
AAGAGGACGT CAAGTATTGC TGATGAGGGC ACGTACACAC TGGACTCCAT CATCCGTCAG 
CTCAACACCT TCCACTCCAT CATGTGTCAC CACGGCACTG ACCCCGAGCT CATCAAACAG 
GTGGTCAAGC AGCAGTTCTA CATCATCGGC GCTGTCACCC TCAACAACCT GCTGCTGCGC 
AAAGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ATAACGTGAG TCAGCTGGAG 
GAATGGCTGA GGGATAAGAA CCTCATGACA TGCGGCGCTA AAGAAACACT GGAGCCGTTG 
ATTCAGGCTG CGCAACTACT GCAGGTGAAG AAGAAAACTG ATGAGGACGC CGAGGCCATC 
TGCTCTATGT GTAACGCCCT CTCCACAGCA CAGATTGTGA AAGTGCTGAA CTTGTACACA 
CCTGTCAATG CATTTGAAGA GCGAGTGTCA GTGTTGTTCA TCAGAACAAT ACAGACTCGT 
TTACGGGATC GAAAGGAGTC GCCTCAGCTT CTGATGGACA CCAAACTCAT CTACCCCGTG 
ACCTTCCCCT TTAACCCCTC CTCCCTTGCC CTGGAAACCA TTCAGATCCC CAGCAGCCTC 
AATCTGGCCT TCCTCACTCG TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_021473331.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329006.1 Danio rerio myosin VAa (myo5aa), transcript variant X4, mRNA.

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CloneID	ODa146054
Clone ID Related Accession (Same CDS sequence)	XM_021473331.1
Accession Version	XM_021473331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395043.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGACGACTAT GACTGAACCT CTGCCATACA TTGAGGATGT GCAGAAGCTC 
ACTGATGCCA AGGAGATCTC ACAGGCCTAC ATGGGCCTCA AAGACACCAA CAGGCTGTTG 
GAGTCTCAGT TGCAGGCTCA ACGTCGCTCT CATGACAACG AGGTGGAAGT TTTGCGTGGA 
GAGCTGCACA GTCTTAAGGA GGAGAACAAC CGTCAGCAGC AGCTGTTGGC GCAGAACCTG 
CAACTACCTC CAGAGGCCAG GATTGAAGCC AGTCTACAGC ACGAGATCAC ACGCCTCACC 
AACGAGAACC TGGAACTTCA CTCTGATGAT CCAAAGGAGT ATAAAGCCTA CAGAATCAGC 
ATGTTACGCA GGATGGTTGA TCTTATGGAG CAGTTGGAGA AACAGGACAA GACCGTTCGT 
AAGCTGAAGA AACAGCTCAA AGTATTTGCT AAGAAGATCA ATGATCTGGA AGGGGGGCAG 
ATGGACGTGT CTCCCGGACA AACGGCTGAC GAGCCTGTTC ATCCGGTGAA CATTCCACGC 
AGAGAGAAAG ATTTCCAGGG CATGCTGGAA TACAAGAAAG AGGATGAACT CAAACTGGTC 
AAAAATATCA TACTTGAGCT GAAGCCTCGT GGTGTTGCAG TGAATCTGAT ACCAGGTCTG 
CCCGCATACA TCCTCTTCAT GTGTCTGCGC CATGCTGACT ACATCAACGA TGACCAGAAA 
GTGCGATCTC TACTCACCTC CGTCATCAAC AGCATCAAGA AGATCCTCAA GAAACGAGGT 
GATGACTTTG AGACTGTTTC CTTCTGGTTG GCCAACACAT GTCGTTTTCT GCACTGCCTG 
AAACAGTACA GTGGTGATGA GCAATTCATG AAGCATAATA GCCCAAAGCA GAACGAACAC 
TGTCTCTCTA ACTTTGACCT GGCAGAGTAC CGGCAGGTTC TGAGCGATCT GGCCATTCAG 
ATCTACCAGC AGCTCATCAA GTGTATGGAA AACATCCTGC AGCCCATGAT CGTCTCTGGC 
ATGCTGGAAC ATGAAACCAT CCAGGGTGTG TCAGGAGTGA AGCCCACAGG TCTCCGCAAG 
AGGACGTCAA GTATTGCTGA TGAGGGCACG TACACACTGG ACTCCATCAT CCGTCAGCTC 
AACACCTTCC ACTCCATCAT GTGTCACCAC GGCACTGACC CCGAGCTCAT CAAACAGGTG 
GTCAAGCAGC AGTTCTACAT CATCGGCGCT GTCACCCTCA ACAACCTGCT GCTGCGCAAA 
GACATGTGCT CCTGGAGCAA GGGCATGCAG ATCAGGTATA ACGTGAGTCA GCTGGAGGAA 
TGGCTGAGGG ATAAGAACCT CATGACATGC GGCGCTAAAG AAACACTGGA GCCGTTGATT 
CAGGCTGCGC AACTACTGCA GGTGAAGAAG AAAACTGATG AGGACGCCGA GGCCATCTGC 
TCTATGTGTA ACGCCCTCTC CACAGCACAG ATTGTGAAAG TGCTGAACTT GTACACACCT 
GTCAATGCAT TTGAAGAGCG AGTGTCAGTG TTGTTCATCA GAACAATACA GACTCGTTTA 
CGGGATCGAA AGGAGTCGCC TCAGCTTCTG ATGGACACCA AACTCATCTA CCCCGTGACC 
TTCCCCTTTA ACCCCTCCTC CCTTGCCCTG GAAACCATTC AGATCCCCAG CAGCCTCAAT 
CTGGCCTTCC TCACTCGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329006.1
CDS	100..5643
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_021473331.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473331.1

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGACGACTAT GACTGAACCT CTGCCATACA TTGAGGATGT GCAGAAGCTC 
ACTGATGCCA AGGAGATCTC ACAGGCCTAC ATGGGCCTCA AAGACACCAA CAGGCTGTTG 
GAGTCTCAGT TGCAGGCTCA ACGTCGCTCT CATGACAACG AGGTGGAAGT TTTGCGTGGA 
GAGCTGCACA GTCTTAAGGA GGAGAACAAC CGTCAGCAGC AGCTGTTGGC GCAGAACCTG 
CAACTACCTC CAGAGGCCAG GATTGAAGCC AGTCTACAGC ACGAGATCAC ACGCCTCACC 
AACGAGAACC TGGAACTTCA CTCTGATGAT CCAAAGGAGT ATAAAGCCTA CAGAATCAGC 
ATGTTACGCA GGATGGTTGA TCTTATGGAG CAGTTGGAGA AACAGGACAA GACCGTTCGT 
AAGCTGAAGA AACAGCTCAA AGTATTTGCT AAGAAGATCA ATGATCTGGA AGGGGGGCAG 
ATGGACGTGT CTCCCGGACA AACGGCTGAC GAGCCTGTTC ATCCGGTGAA CATTCCACGC 
AGAGAGAAAG ATTTCCAGGG CATGCTGGAA TACAAGAAAG AGGATGAACT CAAACTGGTC 
AAAAATATCA TACTTGAGCT GAAGCCTCGT GGTGTTGCAG TGAATCTGAT ACCAGGTCTG 
CCCGCATACA TCCTCTTCAT GTGTCTGCGC CATGCTGACT ACATCAACGA TGACCAGAAA 
GTGCGATCTC TACTCACCTC CGTCATCAAC AGCATCAAGA AGATCCTCAA GAAACGAGGT 
GATGACTTTG AGACTGTTTC CTTCTGGTTG GCCAACACAT GTCGTTTTCT GCACTGCCTG 
AAACAGTACA GTGGTGATGA GCAATTCATG AAGCATAATA GCCCAAAGCA GAACGAACAC 
TGTCTCTCTA ACTTTGACCT GGCAGAGTAC CGGCAGGTTC TGAGCGATCT GGCCATTCAG 
ATCTACCAGC AGCTCATCAA GTGTATGGAA AACATCCTGC AGCCCATGAT CGTCTCTGGC 
ATGCTGGAAC ATGAAACCAT CCAGGGTGTG TCAGGAGTGA AGCCCACAGG TCTCCGCAAG 
AGGACGTCAA GTATTGCTGA TGAGGGCACG TACACACTGG ACTCCATCAT CCGTCAGCTC 
AACACCTTCC ACTCCATCAT GTGTCACCAC GGCACTGACC CCGAGCTCAT CAAACAGGTG 
GTCAAGCAGC AGTTCTACAT CATCGGCGCT GTCACCCTCA ACAACCTGCT GCTGCGCAAA 
GACATGTGCT CCTGGAGCAA GGGCATGCAG ATCAGGTATA ACGTGAGTCA GCTGGAGGAA 
TGGCTGAGGG ATAAGAACCT CATGACATGC GGCGCTAAAG AAACACTGGA GCCGTTGATT 
CAGGCTGCGC AACTACTGCA GGTGAAGAAG AAAACTGATG AGGACGCCGA GGCCATCTGC 
TCTATGTGTA ACGCCCTCTC CACAGCACAG ATTGTGAAAG TGCTGAACTT GTACACACCT 
GTCAATGCAT TTGAAGAGCG AGTGTCAGTG TTGTTCATCA GAACAATACA GACTCGTTTA 
CGGGATCGAA AGGAGTCGCC TCAGCTTCTG ATGGACACCA AACTCATCTA CCCCGTGACC 
TTCCCCTTTA ACCCCTCCTC CCTTGCCCTG GAAACCATTC AGATCCCCAG CAGCCTCAAT 
CTGGCCTTCC TCACTCGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_021473332.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329007.1 Danio rerio myosin VAa (myo5aa), transcript variant X5, mRNA.

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CloneID	ODa146055
Clone ID Related Accession (Same CDS sequence)	XM_021473332.1
Accession Version	XM_021473332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395043.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGA CGACTATGAC TGAACCTCTG CCATACATTG AGGATGTGCA GAAGCTCACT 
GATGCCAAGG AGATCTCACA GGCCTACATG GGCCTCAAAG ACACCAACAG GCTGTTGGAG 
TCTCAGTTGC AGGCTCAACG TCGCTCTCAT GACAACGAGG TGGAAGTTTT GCGTGGAGAG 
CTGCACAGTC TTAAGGAGGA GAACAACCGT CAGCAGCAGC TGTTGGCGCA GAACCTGCAA 
CTACCTCCAG AGGCCAGGAT TGAAGCCAGT CTACAGCACG AGATCACACG CCTCACCAAC 
GAGAACCTGG AACTTCACTC TGATGATCCA AAGGAGTATA AAGCCTACAG AATCAGCATG 
TTACGCAGGA TGGTTGATCT TATGGAGCAG TTGGAGAAAC AGGACAAGAC CGTTCGTAAG 
CTGAAGAAAC AGCTCAAAGT ATTTGCTAAG AAGATCAATG ATCTGGAAGG GGGGCAGATG 
GACGTGTCTC CCGGACAAAC GGCTGACGAG CCTGTTCATC CGGTGAACAT TCCACGCAGA 
GAGAAAGATT TCCAGGGCAT GCTGGAATAC AAGAAAGAGG ATGAACTCAA ACTGGTCAAA 
AATATCATAC TTGAGCTGAA GCCTCGTGGT GTTGCAGTGA ATCTGATACC AGGTCTGCCC 
GCATACATCC TCTTCATGTG TCTGCGCCAT GCTGACTACA TCAACGATGA CCAGAAAGTG 
CGATCTCTAC TCACCTCCGT CATCAACAGC ATCAAGAAGA TCCTCAAGAA ACGAGGTGAT 
GACTTTGAGA CTGTTTCCTT CTGGTTGGCC AACACATGTC GTTTTCTGCA CTGCCTGAAA 
CAGTACAGTG GTGATGAGCA ATTCATGAAG CATAATAGCC CAAAGCAGAA CGAACACTGT 
CTCTCTAACT TTGACCTGGC AGAGTACCGG CAGGTTCTGA GCGATCTGGC CATTCAGATC 
TACCAGCAGC TCATCAAGTG TATGGAAAAC ATCCTGCAGC CCATGATCGT CTCTGGCATG 
CTGGAACATG AAACCATCCA GGGTGTGTCA GGAGTGAAGC CCACAGGTCT CCGCAAGAGG 
ACGTCAAGTA TTGCTGATGA GGGCACGTAC ACACTGGACT CCATCATCCG TCAGCTCAAC 
ACCTTCCACT CCATCATGTG TCACCACGGC ACTGACCCCG AGCTCATCAA ACAGGTGGTC 
AAGCAGCAGT TCTACATCAT CGGCGCTGTC ACCCTCAACA ACCTGCTGCT GCGCAAAGAC 
ATGTGCTCCT GGAGCAAGGG CATGCAGATC AGGTATAACG TGAGTCAGCT GGAGGAATGG 
CTGAGGGATA AGAACCTCAT GACATGCGGC GCTAAAGAAA CACTGGAGCC GTTGATTCAG 
GCTGCGCAAC TACTGCAGGT GAAGAAGAAA ACTGATGAGG ACGCCGAGGC CATCTGCTCT 
ATGTGTAACG CCCTCTCCAC AGCACAGATT GTGAAAGTGC TGAACTTGTA CACACCTGTC 
AATGCATTTG AAGAGCGAGT GTCAGTGTTG TTCATCAGAA CAATACAGAC TCGTTTACGG 
GATCGAAAGG AGTCGCCTCA GCTTCTGATG GACACCAAAC TCATCTACCC CGTGACCTTC 
CCCTTTAACC CCTCCTCCCT TGCCCTGGAA ACCATTCAGA TCCCCAGCAG CCTCAATCTG 
GCCTTCCTCA CTCGTGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329007.1
CDS	100..5640
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_021473332.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473332.1

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGA CGACTATGAC TGAACCTCTG CCATACATTG AGGATGTGCA GAAGCTCACT 
GATGCCAAGG AGATCTCACA GGCCTACATG GGCCTCAAAG ACACCAACAG GCTGTTGGAG 
TCTCAGTTGC AGGCTCAACG TCGCTCTCAT GACAACGAGG TGGAAGTTTT GCGTGGAGAG 
CTGCACAGTC TTAAGGAGGA GAACAACCGT CAGCAGCAGC TGTTGGCGCA GAACCTGCAA 
CTACCTCCAG AGGCCAGGAT TGAAGCCAGT CTACAGCACG AGATCACACG CCTCACCAAC 
GAGAACCTGG AACTTCACTC TGATGATCCA AAGGAGTATA AAGCCTACAG AATCAGCATG 
TTACGCAGGA TGGTTGATCT TATGGAGCAG TTGGAGAAAC AGGACAAGAC CGTTCGTAAG 
CTGAAGAAAC AGCTCAAAGT ATTTGCTAAG AAGATCAATG ATCTGGAAGG GGGGCAGATG 
GACGTGTCTC CCGGACAAAC GGCTGACGAG CCTGTTCATC CGGTGAACAT TCCACGCAGA 
GAGAAAGATT TCCAGGGCAT GCTGGAATAC AAGAAAGAGG ATGAACTCAA ACTGGTCAAA 
AATATCATAC TTGAGCTGAA GCCTCGTGGT GTTGCAGTGA ATCTGATACC AGGTCTGCCC 
GCATACATCC TCTTCATGTG TCTGCGCCAT GCTGACTACA TCAACGATGA CCAGAAAGTG 
CGATCTCTAC TCACCTCCGT CATCAACAGC ATCAAGAAGA TCCTCAAGAA ACGAGGTGAT 
GACTTTGAGA CTGTTTCCTT CTGGTTGGCC AACACATGTC GTTTTCTGCA CTGCCTGAAA 
CAGTACAGTG GTGATGAGCA ATTCATGAAG CATAATAGCC CAAAGCAGAA CGAACACTGT 
CTCTCTAACT TTGACCTGGC AGAGTACCGG CAGGTTCTGA GCGATCTGGC CATTCAGATC 
TACCAGCAGC TCATCAAGTG TATGGAAAAC ATCCTGCAGC CCATGATCGT CTCTGGCATG 
CTGGAACATG AAACCATCCA GGGTGTGTCA GGAGTGAAGC CCACAGGTCT CCGCAAGAGG 
ACGTCAAGTA TTGCTGATGA GGGCACGTAC ACACTGGACT CCATCATCCG TCAGCTCAAC 
ACCTTCCACT CCATCATGTG TCACCACGGC ACTGACCCCG AGCTCATCAA ACAGGTGGTC 
AAGCAGCAGT TCTACATCAT CGGCGCTGTC ACCCTCAACA ACCTGCTGCT GCGCAAAGAC 
ATGTGCTCCT GGAGCAAGGG CATGCAGATC AGGTATAACG TGAGTCAGCT GGAGGAATGG 
CTGAGGGATA AGAACCTCAT GACATGCGGC GCTAAAGAAA CACTGGAGCC GTTGATTCAG 
GCTGCGCAAC TACTGCAGGT GAAGAAGAAA ACTGATGAGG ACGCCGAGGC CATCTGCTCT 
ATGTGTAACG CCCTCTCCAC AGCACAGATT GTGAAAGTGC TGAACTTGTA CACACCTGTC 
AATGCATTTG AAGAGCGAGT GTCAGTGTTG TTCATCAGAA CAATACAGAC TCGTTTACGG 
GATCGAAAGG AGTCGCCTCA GCTTCTGATG GACACCAAAC TCATCTACCC CGTGACCTTC 
CCCTTTAACC CCTCCTCCCT TGCCCTGGAA ACCATTCAGA TCCCCAGCAG CCTCAATCTG 
GCCTTCCTCA CTCGTGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_021473333.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329008.1 Danio rerio myosin VAa (myo5aa), transcript variant X6, mRNA.

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CloneID	ODa146056
Clone ID Related Accession (Same CDS sequence)	XM_021473333.1
Accession Version	XM_021473333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGGCTGTTGG AGTCTCAGTT GCAGGCTCAA CGTCGCTCTC ATGACAACGA GGTGGAAGTT 
TTGCGTGGAG AGCTGCACAG TCTTAAGGAG GAGAACAACC GTCAGCAGCA GCTGTTGGCG 
CAGAACCTGC AACTACCTCC AGAGGCCAGG ATTGAAGCCA GTCTACAGCA CGAGATCACA 
CGCCTCACCA ACGAGAACCT GGATCTTATG GAGCAGTTGG AGAAACAGGA CAAGACCGTT 
CGTAAGCTGA AGAAACAGCT CAAAGTATTT GCTAAGAAGA TCAATGATCT GGAAGGGGGG 
CAGATGGACG TGTCTCCCGG ACAAACGGCT GACGAGCCTG TTCATCCGGT GAACATTCCA 
CGCAGAGAGA AAGATTTCCA GGGCATGCTG GAATACAAGA AAGAGGATGA ACTCAAACTG 
GTCAAAAATA TCATACTTGA GCTGAAGCCT CGTGGTGTTG CAGTGAATCT GATACCAGGT 
CTGCCCGCAT ACATCCTCTT CATGTGTCTG CGCCATGCTG ACTACATCAA CGATGACCAG 
AAAGTGCGAT CTCTACTCAC CTCCGTCATC AACAGCATCA AGAAGATCCT CAAGAAACGA 
GGTGATGACT TTGAGACTGT TTCCTTCTGG TTGGCCAACA CATGTCGTTT TCTGCACTGC 
CTGAAACAGT ACAGTGGTGA TGAGCAATTC ATGAAGCATA ATAGCCCAAA GCAGAACGAA 
CACTGTCTCT CTAACTTTGA CCTGGCAGAG TACCGGCAGG TTCTGAGCGA TCTGGCCATT 
CAGATCTACC AGCAGCTCAT CAAGTGTATG GAAAACATCC TGCAGCCCAT GATCGTCTCT 
GGCATGCTGG AACATGAAAC CATCCAGGGT GTGTCAGGAG TGAAGCCCAC AGGTCTCCGC 
AAGAGGACGT CAAGTATTGC TGATGAGGGC ACGTACACAC TGGACTCCAT CATCCGTCAG 
CTCAACACCT TCCACTCCAT CATGTGTCAC CACGGCACTG ACCCCGAGCT CATCAAACAG 
GTGGTCAAGC AGCAGTTCTA CATCATCGGC GCTGTCACCC TCAACAACCT GCTGCTGCGC 
AAAGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ATAACGTGAG TCAGCTGGAG 
GAATGGCTGA GGGATAAGAA CCTCATGACA TGCGGCGCTA AAGAAACACT GGAGCCGTTG 
ATTCAGGCTG CGCAACTACT GCAGGTGAAG AAGAAAACTG ATGAGGACGC CGAGGCCATC 
TGCTCTATGT GTAACGCCCT CTCCACAGCA CAGATTGTGA AAGTGCTGAA CTTGTACACA 
CCTGTCAATG CATTTGAAGA GCGAGTGTCA GTGTTGTTCA TCAGAACAAT ACAGACTCGT 
TTACGGGATC GAAAGGAGTC GCCTCAGCTT CTGATGGACA CCAAACTCAT CTACCCCGTG 
ACCTTCCCCT TTAACCCCTC CTCCCTTGCC CTGGAAACCA TTCAGATCCC CAGCAGCCTC 
AATCTGGCCT TCCTCACTCG TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329008.1
CDS	100..5586
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_021473333.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473333.1

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGGCTGTTGG AGTCTCAGTT GCAGGCTCAA CGTCGCTCTC ATGACAACGA GGTGGAAGTT 
TTGCGTGGAG AGCTGCACAG TCTTAAGGAG GAGAACAACC GTCAGCAGCA GCTGTTGGCG 
CAGAACCTGC AACTACCTCC AGAGGCCAGG ATTGAAGCCA GTCTACAGCA CGAGATCACA 
CGCCTCACCA ACGAGAACCT GGATCTTATG GAGCAGTTGG AGAAACAGGA CAAGACCGTT 
CGTAAGCTGA AGAAACAGCT CAAAGTATTT GCTAAGAAGA TCAATGATCT GGAAGGGGGG 
CAGATGGACG TGTCTCCCGG ACAAACGGCT GACGAGCCTG TTCATCCGGT GAACATTCCA 
CGCAGAGAGA AAGATTTCCA GGGCATGCTG GAATACAAGA AAGAGGATGA ACTCAAACTG 
GTCAAAAATA TCATACTTGA GCTGAAGCCT CGTGGTGTTG CAGTGAATCT GATACCAGGT 
CTGCCCGCAT ACATCCTCTT CATGTGTCTG CGCCATGCTG ACTACATCAA CGATGACCAG 
AAAGTGCGAT CTCTACTCAC CTCCGTCATC AACAGCATCA AGAAGATCCT CAAGAAACGA 
GGTGATGACT TTGAGACTGT TTCCTTCTGG TTGGCCAACA CATGTCGTTT TCTGCACTGC 
CTGAAACAGT ACAGTGGTGA TGAGCAATTC ATGAAGCATA ATAGCCCAAA GCAGAACGAA 
CACTGTCTCT CTAACTTTGA CCTGGCAGAG TACCGGCAGG TTCTGAGCGA TCTGGCCATT 
CAGATCTACC AGCAGCTCAT CAAGTGTATG GAAAACATCC TGCAGCCCAT GATCGTCTCT 
GGCATGCTGG AACATGAAAC CATCCAGGGT GTGTCAGGAG TGAAGCCCAC AGGTCTCCGC 
AAGAGGACGT CAAGTATTGC TGATGAGGGC ACGTACACAC TGGACTCCAT CATCCGTCAG 
CTCAACACCT TCCACTCCAT CATGTGTCAC CACGGCACTG ACCCCGAGCT CATCAAACAG 
GTGGTCAAGC AGCAGTTCTA CATCATCGGC GCTGTCACCC TCAACAACCT GCTGCTGCGC 
AAAGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ATAACGTGAG TCAGCTGGAG 
GAATGGCTGA GGGATAAGAA CCTCATGACA TGCGGCGCTA AAGAAACACT GGAGCCGTTG 
ATTCAGGCTG CGCAACTACT GCAGGTGAAG AAGAAAACTG ATGAGGACGC CGAGGCCATC 
TGCTCTATGT GTAACGCCCT CTCCACAGCA CAGATTGTGA AAGTGCTGAA CTTGTACACA 
CCTGTCAATG CATTTGAAGA GCGAGTGTCA GTGTTGTTCA TCAGAACAAT ACAGACTCGT 
TTACGGGATC GAAAGGAGTC GCCTCAGCTT CTGATGGACA CCAAACTCAT CTACCCCGTG 
ACCTTCCCCT TTAACCCCTC CTCCCTTGCC CTGGAAACCA TTCAGATCCC CAGCAGCCTC 
AATCTGGCCT TCCTCACTCG TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_021473334.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329009.1 Danio rerio myosin VAa (myo5aa), transcript variant X7, mRNA.

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CloneID	ODa146057
Clone ID Related Accession (Same CDS sequence)	XM_021473334.1
Accession Version	XM_021473334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGG 
CTGTTGGAGT CTCAGTTGCA GGCTCAACGT CGCTCTCATG ACAACGAGGT GGAAGTTTTG 
CGTGGAGAGC TGCACAGTCT TAAGGAGGAG AACAACCGTC AGCAGCAGCT GTTGGCGCAG 
AACCTGCAAC TACCTCCAGA GGCCAGGATT GAAGCCAGTC TACAGCACGA GATCACACGC 
CTCACCAACG AGAACCTGGA TCTTATGGAG CAGTTGGAGA AACAGGACAA GACCGTTCGT 
AAGCTGAAGA AACAGCTCAA AGTATTTGCT AAGAAGATCA ATGATCTGGA AGGGGGGCAG 
ATGGACGTGT CTCCCGGACA AACGGCTGAC GAGCCTGTTC ATCCGGTGAA CATTCCACGC 
AGAGAGAAAG ATTTCCAGGG CATGCTGGAA TACAAGAAAG AGGATGAACT CAAACTGGTC 
AAAAATATCA TACTTGAGCT GAAGCCTCGT GGTGTTGCAG TGAATCTGAT ACCAGGTCTG 
CCCGCATACA TCCTCTTCAT GTGTCTGCGC CATGCTGACT ACATCAACGA TGACCAGAAA 
GTGCGATCTC TACTCACCTC CGTCATCAAC AGCATCAAGA AGATCCTCAA GAAACGAGGT 
GATGACTTTG AGACTGTTTC CTTCTGGTTG GCCAACACAT GTCGTTTTCT GCACTGCCTG 
AAACAGTACA GTGGTGATGA GCAATTCATG AAGCATAATA GCCCAAAGCA GAACGAACAC 
TGTCTCTCTA ACTTTGACCT GGCAGAGTAC CGGCAGGTTC TGAGCGATCT GGCCATTCAG 
ATCTACCAGC AGCTCATCAA GTGTATGGAA AACATCCTGC AGCCCATGAT CGTCTCTGGC 
ATGCTGGAAC ATGAAACCAT CCAGGGTGTG TCAGGAGTGA AGCCCACAGG TCTCCGCAAG 
AGGACGTCAA GTATTGCTGA TGAGGGCACG TACACACTGG ACTCCATCAT CCGTCAGCTC 
AACACCTTCC ACTCCATCAT GTGTCACCAC GGCACTGACC CCGAGCTCAT CAAACAGGTG 
GTCAAGCAGC AGTTCTACAT CATCGGCGCT GTCACCCTCA ACAACCTGCT GCTGCGCAAA 
GACATGTGCT CCTGGAGCAA GGGCATGCAG ATCAGGTATA ACGTGAGTCA GCTGGAGGAA 
TGGCTGAGGG ATAAGAACCT CATGACATGC GGCGCTAAAG AAACACTGGA GCCGTTGATT 
CAGGCTGCGC AACTACTGCA GGTGAAGAAG AAAACTGATG AGGACGCCGA GGCCATCTGC 
TCTATGTGTA ACGCCCTCTC CACAGCACAG ATTGTGAAAG TGCTGAACTT GTACACACCT 
GTCAATGCAT TTGAAGAGCG AGTGTCAGTG TTGTTCATCA GAACAATACA GACTCGTTTA 
CGGGATCGAA AGGAGTCGCC TCAGCTTCTG ATGGACACCA AACTCATCTA CCCCGTGACC 
TTCCCCTTTA ACCCCTCCTC CCTTGCCCTG GAAACCATTC AGATCCCCAG CAGCCTCAAT 
CTGGCCTTCC TCACTCGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329009.1
CDS	100..5583
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_021473334.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473334.1

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGG 
CTGTTGGAGT CTCAGTTGCA GGCTCAACGT CGCTCTCATG ACAACGAGGT GGAAGTTTTG 
CGTGGAGAGC TGCACAGTCT TAAGGAGGAG AACAACCGTC AGCAGCAGCT GTTGGCGCAG 
AACCTGCAAC TACCTCCAGA GGCCAGGATT GAAGCCAGTC TACAGCACGA GATCACACGC 
CTCACCAACG AGAACCTGGA TCTTATGGAG CAGTTGGAGA AACAGGACAA GACCGTTCGT 
AAGCTGAAGA AACAGCTCAA AGTATTTGCT AAGAAGATCA ATGATCTGGA AGGGGGGCAG 
ATGGACGTGT CTCCCGGACA AACGGCTGAC GAGCCTGTTC ATCCGGTGAA CATTCCACGC 
AGAGAGAAAG ATTTCCAGGG CATGCTGGAA TACAAGAAAG AGGATGAACT CAAACTGGTC 
AAAAATATCA TACTTGAGCT GAAGCCTCGT GGTGTTGCAG TGAATCTGAT ACCAGGTCTG 
CCCGCATACA TCCTCTTCAT GTGTCTGCGC CATGCTGACT ACATCAACGA TGACCAGAAA 
GTGCGATCTC TACTCACCTC CGTCATCAAC AGCATCAAGA AGATCCTCAA GAAACGAGGT 
GATGACTTTG AGACTGTTTC CTTCTGGTTG GCCAACACAT GTCGTTTTCT GCACTGCCTG 
AAACAGTACA GTGGTGATGA GCAATTCATG AAGCATAATA GCCCAAAGCA GAACGAACAC 
TGTCTCTCTA ACTTTGACCT GGCAGAGTAC CGGCAGGTTC TGAGCGATCT GGCCATTCAG 
ATCTACCAGC AGCTCATCAA GTGTATGGAA AACATCCTGC AGCCCATGAT CGTCTCTGGC 
ATGCTGGAAC ATGAAACCAT CCAGGGTGTG TCAGGAGTGA AGCCCACAGG TCTCCGCAAG 
AGGACGTCAA GTATTGCTGA TGAGGGCACG TACACACTGG ACTCCATCAT CCGTCAGCTC 
AACACCTTCC ACTCCATCAT GTGTCACCAC GGCACTGACC CCGAGCTCAT CAAACAGGTG 
GTCAAGCAGC AGTTCTACAT CATCGGCGCT GTCACCCTCA ACAACCTGCT GCTGCGCAAA 
GACATGTGCT CCTGGAGCAA GGGCATGCAG ATCAGGTATA ACGTGAGTCA GCTGGAGGAA 
TGGCTGAGGG ATAAGAACCT CATGACATGC GGCGCTAAAG AAACACTGGA GCCGTTGATT 
CAGGCTGCGC AACTACTGCA GGTGAAGAAG AAAACTGATG AGGACGCCGA GGCCATCTGC 
TCTATGTGTA ACGCCCTCTC CACAGCACAG ATTGTGAAAG TGCTGAACTT GTACACACCT 
GTCAATGCAT TTGAAGAGCG AGTGTCAGTG TTGTTCATCA GAACAATACA GACTCGTTTA 
CGGGATCGAA AGGAGTCGCC TCAGCTTCTG ATGGACACCA AACTCATCTA CCCCGTGACC 
TTCCCCTTTA ACCCCTCCTC CCTTGCCCTG GAAACCATTC AGATCCCCAG CAGCCTCAAT 
CTGGCCTTCC TCACTCGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_021473335.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329010.1 Danio rerio myosin VAa (myo5aa), transcript variant X8, mRNA.

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CloneID	ODa146058
Clone ID Related Accession (Same CDS sequence)	XM_021473335.1
Accession Version	XM_021473335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGACGACTAT GACTGAACCT CTGCCATACA TTGAGGATGT GCAGAAGCTC 
ACTGATGCCA AGGAGATCTC ACAGGCCTAC ATGGGCCTCA AAGACACCAA CAGGCTGTTG 
GAGTCTCAGT TGCAGGCTCA ACGTCGCTCT CATGACAACG AGGTGGAAGT TTTGCGTGGA 
GAGCTGCACA GTCTTAAGGA GGAGAACAAC CGTCAGCAGC AGCTGTTGGC GCAGAACCTG 
CAACTACCTC CAGAGGCCAG GATTGAAGCC AGTCTACAGC ACGAGATCAC ACGCCTCACC 
AACGAGAACC TGGATCTTAT GGAGCAGTTG GAGAAACAGG ACAAGACCGT TCGTAAGCTG 
AAGAAACAGC TCAAAGTATT TGCTAAGAAG ATCAATGATC TGGAAGGGGG GCAGATGGAC 
GTGTCTCCCG GACAAACGGC TGACGAGCCT GTTCATCCGG TGAACATTCC ACGCAGAGAG 
AAAGATTTCC AGGGCATGCT GGAATACAAG AAAGAGGATG AACTCAAACT GGTCAAAAAT 
ATCATACTTG AGCTGAAGCC TCGTGGTGTT GCAGTGAATC TGATACCAGG TCTGCCCGCA 
TACATCCTCT TCATGTGTCT GCGCCATGCT GACTACATCA ACGATGACCA GAAAGTGCGA 
TCTCTACTCA CCTCCGTCAT CAACAGCATC AAGAAGATCC TCAAGAAACG AGGTGATGAC 
TTTGAGACTG TTTCCTTCTG GTTGGCCAAC ACATGTCGTT TTCTGCACTG CCTGAAACAG 
TACAGTGGTG ATGAGCAATT CATGAAGCAT AATAGCCCAA AGCAGAACGA ACACTGTCTC 
TCTAACTTTG ACCTGGCAGA GTACCGGCAG GTTCTGAGCG ATCTGGCCAT TCAGATCTAC 
CAGCAGCTCA TCAAGTGTAT GGAAAACATC CTGCAGCCCA TGATCGTCTC TGGCATGCTG 
GAACATGAAA CCATCCAGGG TGTGTCAGGA GTGAAGCCCA CAGGTCTCCG CAAGAGGACG 
TCAAGTATTG CTGATGAGGG CACGTACACA CTGGACTCCA TCATCCGTCA GCTCAACACC 
TTCCACTCCA TCATGTGTCA CCACGGCACT GACCCCGAGC TCATCAAACA GGTGGTCAAG 
CAGCAGTTCT ACATCATCGG CGCTGTCACC CTCAACAACC TGCTGCTGCG CAAAGACATG 
TGCTCCTGGA GCAAGGGCAT GCAGATCAGG TATAACGTGA GTCAGCTGGA GGAATGGCTG 
AGGGATAAGA ACCTCATGAC ATGCGGCGCT AAAGAAACAC TGGAGCCGTT GATTCAGGCT 
GCGCAACTAC TGCAGGTGAA GAAGAAAACT GATGAGGACG CCGAGGCCAT CTGCTCTATG 
TGTAACGCCC TCTCCACAGC ACAGATTGTG AAAGTGCTGA ACTTGTACAC ACCTGTCAAT 
GCATTTGAAG AGCGAGTGTC AGTGTTGTTC ATCAGAACAA TACAGACTCG TTTACGGGAT 
CGAAAGGAGT CGCCTCAGCT TCTGATGGAC ACCAAACTCA TCTACCCCGT GACCTTCCCC 
TTTAACCCCT CCTCCCTTGC CCTGGAAACC ATTCAGATCC CCAGCAGCCT CAATCTGGCC 
TTCCTCACTC GTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329010.1
CDS	100..5577
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_021473335.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473335.1

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGACGACTAT GACTGAACCT CTGCCATACA TTGAGGATGT GCAGAAGCTC 
ACTGATGCCA AGGAGATCTC ACAGGCCTAC ATGGGCCTCA AAGACACCAA CAGGCTGTTG 
GAGTCTCAGT TGCAGGCTCA ACGTCGCTCT CATGACAACG AGGTGGAAGT TTTGCGTGGA 
GAGCTGCACA GTCTTAAGGA GGAGAACAAC CGTCAGCAGC AGCTGTTGGC GCAGAACCTG 
CAACTACCTC CAGAGGCCAG GATTGAAGCC AGTCTACAGC ACGAGATCAC ACGCCTCACC 
AACGAGAACC TGGATCTTAT GGAGCAGTTG GAGAAACAGG ACAAGACCGT TCGTAAGCTG 
AAGAAACAGC TCAAAGTATT TGCTAAGAAG ATCAATGATC TGGAAGGGGG GCAGATGGAC 
GTGTCTCCCG GACAAACGGC TGACGAGCCT GTTCATCCGG TGAACATTCC ACGCAGAGAG 
AAAGATTTCC AGGGCATGCT GGAATACAAG AAAGAGGATG AACTCAAACT GGTCAAAAAT 
ATCATACTTG AGCTGAAGCC TCGTGGTGTT GCAGTGAATC TGATACCAGG TCTGCCCGCA 
TACATCCTCT TCATGTGTCT GCGCCATGCT GACTACATCA ACGATGACCA GAAAGTGCGA 
TCTCTACTCA CCTCCGTCAT CAACAGCATC AAGAAGATCC TCAAGAAACG AGGTGATGAC 
TTTGAGACTG TTTCCTTCTG GTTGGCCAAC ACATGTCGTT TTCTGCACTG CCTGAAACAG 
TACAGTGGTG ATGAGCAATT CATGAAGCAT AATAGCCCAA AGCAGAACGA ACACTGTCTC 
TCTAACTTTG ACCTGGCAGA GTACCGGCAG GTTCTGAGCG ATCTGGCCAT TCAGATCTAC 
CAGCAGCTCA TCAAGTGTAT GGAAAACATC CTGCAGCCCA TGATCGTCTC TGGCATGCTG 
GAACATGAAA CCATCCAGGG TGTGTCAGGA GTGAAGCCCA CAGGTCTCCG CAAGAGGACG 
TCAAGTATTG CTGATGAGGG CACGTACACA CTGGACTCCA TCATCCGTCA GCTCAACACC 
TTCCACTCCA TCATGTGTCA CCACGGCACT GACCCCGAGC TCATCAAACA GGTGGTCAAG 
CAGCAGTTCT ACATCATCGG CGCTGTCACC CTCAACAACC TGCTGCTGCG CAAAGACATG 
TGCTCCTGGA GCAAGGGCAT GCAGATCAGG TATAACGTGA GTCAGCTGGA GGAATGGCTG 
AGGGATAAGA ACCTCATGAC ATGCGGCGCT AAAGAAACAC TGGAGCCGTT GATTCAGGCT 
GCGCAACTAC TGCAGGTGAA GAAGAAAACT GATGAGGACG CCGAGGCCAT CTGCTCTATG 
TGTAACGCCC TCTCCACAGC ACAGATTGTG AAAGTGCTGA ACTTGTACAC ACCTGTCAAT 
GCATTTGAAG AGCGAGTGTC AGTGTTGTTC ATCAGAACAA TACAGACTCG TTTACGGGAT 
CGAAAGGAGT CGCCTCAGCT TCTGATGGAC ACCAAACTCA TCTACCCCGT GACCTTCCCC 
TTTAACCCCT CCTCCCTTGC CCTGGAAACC ATTCAGATCC CCAGCAGCCT CAATCTGGCC 
TTCCTCACTC GTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_021473338.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329013.1 Danio rerio myosin VAa (myo5aa), transcript variant X11, mRNA.

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CloneID	ODa146061
Clone ID Related Accession (Same CDS sequence)	XM_021473338.1
Accession Version	XM_021473338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGA 
TCCCCATTCC CTGACTACCA TAAGCTGAAT GAGGATGGAG AACTGTGGCT GGTTTATGAA 
GGGTTAAAGG AAACTAACAG GCTGTTGGAG TCTCAGTTGC AGGCTCAACG TCGCTCTCAT 
GACAACGAGG TGGAAGTTTT GCGTGGAGAG CTGCACAGTC TTAAGGAGGA GAACAACCGT 
CAGCAGCAGC TGTTGGCGCA GAACCTGCAA CTACCTCCAG AGGCCAGGAT TGAAGCCAGT 
CTACAGCACG AGATCACACG CCTCACCAAC GAGAACCTGG ATCTTATGGA GCAGTTGGAG 
AAACAGGACA AGACCGTTCG TAAGCTGAAG AAACAGCTCA AAGTATTTGC TAAGAAGATC 
AATGATCTGG AAGGGGGGCA GATGGACGTG TCTCCCGGAC AAACGGCTGA CGAGCCTGTT 
CATCCGGTGA ACATTCCACG CAGAGAGAAA GATTTCCAGG GCATGCTGGA ATACAAGAAA 
GAGGATGAAC TCAAACTGGT CAAAAATATC ATACTTGAGC TGAAGCCTCG TGGTGTTGCA 
GTGAATCTGA TACCAGGTCT GCCCGCATAC ATCCTCTTCA TGTGTCTGCG CCATGCTGAC 
TACATCAACG ATGACCAGAA AGTGCGATCT CTACTCACCT CCGTCATCAA CAGCATCAAG 
AAGATCCTCA AGAAACGAGG TGATGACTTT GAGACTGTTT CCTTCTGGTT GGCCAACACA 
TGTCGTTTTC TGCACTGCCT GAAACAGTAC AGTGGTGATG AGCAATTCAT GAAGCATAAT 
AGCCCAAAGC AGAACGAACA CTGTCTCTCT AACTTTGACC TGGCAGAGTA CCGGCAGGTT 
CTGAGCGATC TGGCCATTCA GATCTACCAG CAGCTCATCA AGTGTATGGA AAACATCCTG 
CAGCCCATGA TCGTCTCTGG CATGCTGGAA CATGAAACCA TCCAGGGTGT GTCAGGAGTG 
AAGCCCACAG GTCTCCGCAA GAGGACGTCA AGTATTGCTG ATGAGGGCAC GTACACACTG 
GACTCCATCA TCCGTCAGCT CAACACCTTC CACTCCATCA TGTGTCACCA CGGCACTGAC 
CCCGAGCTCA TCAAACAGGT GGTCAAGCAG CAGTTCTACA TCATCGGCGC TGTCACCCTC 
AACAACCTGC TGCTGCGCAA AGACATGTGC TCCTGGAGCA AGGGCATGCA GATCAGGTAT 
AACGTGAGTC AGCTGGAGGA ATGGCTGAGG GATAAGAACC TCATGACATG CGGCGCTAAA 
GAAACACTGG AGCCGTTGAT TCAGGCTGCG CAACTACTGC AGGTGAAGAA GAAAACTGAT 
GAGGACGCCG AGGCCATCTG CTCTATGTGT AACGCCCTCT CCACAGCACA GATTGTGAAA 
GTGCTGAACT TGTACACACC TGTCAATGCA TTTGAAGAGC GAGTGTCAGT GTTGTTCATC 
AGAACAATAC AGACTCGTTT ACGGGATCGA AAGGAGTCGC CTCAGCTTCT GATGGACACC 
AAACTCATCT ACCCCGTGAC CTTCCCCTTT AACCCCTCCT CCCTTGCCCT GGAAACCATT 
CAGATCCCCA GCAGCCTCAA TCTGGCCTTC CTCACTCGTG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329013.1
CDS	100..5664
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRSPFPDYHKLNEDGELWLVYEGLKETNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_021473338.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473338.1

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TCATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGGAAGG CTAATGATTT CCACTACACC AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGTT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTGACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTGGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCCTGGAC TCTCATTGAT TTCTATGATA ATCAGCCGTG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGA 
TCCCCATTCC CTGACTACCA TAAGCTGAAT GAGGATGGAG AACTGTGGCT GGTTTATGAA 
GGGTTAAAGG AAACTAACAG GCTGTTGGAG TCTCAGTTGC AGGCTCAACG TCGCTCTCAT 
GACAACGAGG TGGAAGTTTT GCGTGGAGAG CTGCACAGTC TTAAGGAGGA GAACAACCGT 
CAGCAGCAGC TGTTGGCGCA GAACCTGCAA CTACCTCCAG AGGCCAGGAT TGAAGCCAGT 
CTACAGCACG AGATCACACG CCTCACCAAC GAGAACCTGG ATCTTATGGA GCAGTTGGAG 
AAACAGGACA AGACCGTTCG TAAGCTGAAG AAACAGCTCA AAGTATTTGC TAAGAAGATC 
AATGATCTGG AAGGGGGGCA GATGGACGTG TCTCCCGGAC AAACGGCTGA CGAGCCTGTT 
CATCCGGTGA ACATTCCACG CAGAGAGAAA GATTTCCAGG GCATGCTGGA ATACAAGAAA 
GAGGATGAAC TCAAACTGGT CAAAAATATC ATACTTGAGC TGAAGCCTCG TGGTGTTGCA 
GTGAATCTGA TACCAGGTCT GCCCGCATAC ATCCTCTTCA TGTGTCTGCG CCATGCTGAC 
TACATCAACG ATGACCAGAA AGTGCGATCT CTACTCACCT CCGTCATCAA CAGCATCAAG 
AAGATCCTCA AGAAACGAGG TGATGACTTT GAGACTGTTT CCTTCTGGTT GGCCAACACA 
TGTCGTTTTC TGCACTGCCT GAAACAGTAC AGTGGTGATG AGCAATTCAT GAAGCATAAT 
AGCCCAAAGC AGAACGAACA CTGTCTCTCT AACTTTGACC TGGCAGAGTA CCGGCAGGTT 
CTGAGCGATC TGGCCATTCA GATCTACCAG CAGCTCATCA AGTGTATGGA AAACATCCTG 
CAGCCCATGA TCGTCTCTGG CATGCTGGAA CATGAAACCA TCCAGGGTGT GTCAGGAGTG 
AAGCCCACAG GTCTCCGCAA GAGGACGTCA AGTATTGCTG ATGAGGGCAC GTACACACTG 
GACTCCATCA TCCGTCAGCT CAACACCTTC CACTCCATCA TGTGTCACCA CGGCACTGAC 
CCCGAGCTCA TCAAACAGGT GGTCAAGCAG CAGTTCTACA TCATCGGCGC TGTCACCCTC 
AACAACCTGC TGCTGCGCAA AGACATGTGC TCCTGGAGCA AGGGCATGCA GATCAGGTAT 
AACGTGAGTC AGCTGGAGGA ATGGCTGAGG GATAAGAACC TCATGACATG CGGCGCTAAA 
GAAACACTGG AGCCGTTGAT TCAGGCTGCG CAACTACTGC AGGTGAAGAA GAAAACTGAT 
GAGGACGCCG AGGCCATCTG CTCTATGTGT AACGCCCTCT CCACAGCACA GATTGTGAAA 
GTGCTGAACT TGTACACACC TGTCAATGCA TTTGAAGAGC GAGTGTCAGT GTTGTTCATC 
AGAACAATAC AGACTCGTTT ACGGGATCGA AAGGAGTCGC CTCAGCTTCT GATGGACACC 
AAACTCATCT ACCCCGTGAC CTTCCCCTTT AACCCCTCCT CCCTTGCCCT GGAAACCATT 
CAGATCCCCA GCAGCCTCAA TCTGGCCTTC CTCACTCGTG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_017352118.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207607.1 Danio rerio myosin VAa (myo5aa), transcript variant X11, mRNA.

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CloneID	ODa55977
Clone ID Related Accession (Same CDS sequence)	XM_017352118.2 , XM_017352118.1
Accession Version	XM_017352118.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGA 
TCCCCATTCC CTGACTACCA TAAGCTGAAT GAGGATGGAG AACTGTGGCT GGTTTATGAA 
GGGTTAAAGG AAACTAACAG GCTGTTGGAG TCTCAGTTGC AGGCTCAACG TCGCTCTCAT 
GACAACGAGG TGGAAGTTTT GCGTGGAGAG CTGCACAGTC TTAAGGAGGA GAACAACCGT 
CAGCAGCAGC TGTTGGCGCA GAACCTGCAA CTACCTCCAG AGGCCAGGAT TGAAGCCAGT 
CTACAGCACG AGATCACACG CCTCACCAAC GAGAACCTGG ATCTTATGGA GCAGTTGGAG 
AAACAGGACA AGACCGTTCG TAAGCTGAAG AAACAGCTCA AAGTATTTGC TAAGAAGATC 
AATGATCTGG AAGGGGGGCA GATGGACGTG TCTCCCGGAC AAACGGCTGA CGAGCCTGTT 
CATCCGGTGA ACATTCCACG CAGAGAGAAA GATTTCCAGG GCATGCTGGA ATACAAGAAA 
GAGGATGAAC TCAAACTGGT CAAAAATATC ATACTTGAGC TGAAGCCTCG TGGTGTTGCA 
GTGAATCTGA TACCAGGTCT GCCCGCATAC ATCCTCTTCA TGTGTCTGCG CCATGCTGAC 
TACATCAACG ATGACCAGAA AGTGCGATCT CTACTCACCT CCGTCATCAA CAGCATCAAG 
AAGATCCTCA AGAAACGAGG TGATGACTTT GAGACTGTTT CCTTCTGGTT GGCCAACACA 
TGTCGTTTTC TGCACTGCCT GAAACAGTAC AGTGGTGATG AGCAATTCAT GAAGCATAAT 
AGCCCAAAGC AGAACGAACA CTGTCTCTCT AACTTTGACC TGGCAGAGTA CCGGCAGGTT 
CTGAGCGATC TGGCCATTCA GATCTACCAG CAGCTCATCA AGTGTATGGA AAACATCCTG 
CAGCCCATGA TCGTCTCTGG CATGCTGGAA CATGAAACCA TCCAGGGTGT GTCAGGAGTG 
AAGCCCACAG GTCTCCGCAA GAGGACGTCA AGTATTGCTG ATGAGGGCAC GTACACACTG 
GACTCCATCA TCCGTCAGCT CAACACCTTC CACTCCATCA TGTGTCACCA CGGCACTGAC 
CCCGAGCTCA TCAAACAGGT GGTCAAGCAG CAGTTCTACA TCATCGGCGC TGTCACCCTC 
AACAACCTGC TGCTGCGCAA AGACATGTGC TCCTGGAGCA AGGGCATGCA GATCAGGTAT 
AACGTGAGTC AGCTGGAGGA ATGGCTGAGG GATAAGAACC TCATGACATG CGGCGCTAAA 
GAAACACTGG AGCCGTTGAT TCAGGCTGCG CAACTACTGC AGGTGAAGAA GAAAACTGAT 
GAGGACGCCG AGGCCATCTG CTCTATGTGT AACGCCCTCT CCACAGCACA GATTGTGAAA 
GTGCTGAACT TGTACACACC TGTCAATGCA TTTGAAGAGC GAGTGTCAGT GTTGTTCATC 
AGAACAATAC AGACTCGTTT ACGGGATCGA AAGGAGTCGC CTCAGCTTCT GATGGACACC 
AAACTCATCT ACCCCGTGAC CTTCCCCTTT AACCCCTCCT CCCTTGCCCT GGAAACCATT 
CAGATCCCCA GCAGCCTCAA TCTGGCCTTC CTCACTCGTG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207607.1
CDS	165..5729
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRSPFPDYHKLNEDGELWLVYEGLKETNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_017352118.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352118.2

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGA 
TCCCCATTCC CTGACTACCA TAAGCTGAAT GAGGATGGAG AACTGTGGCT GGTTTATGAA 
GGGTTAAAGG AAACTAACAG GCTGTTGGAG TCTCAGTTGC AGGCTCAACG TCGCTCTCAT 
GACAACGAGG TGGAAGTTTT GCGTGGAGAG CTGCACAGTC TTAAGGAGGA GAACAACCGT 
CAGCAGCAGC TGTTGGCGCA GAACCTGCAA CTACCTCCAG AGGCCAGGAT TGAAGCCAGT 
CTACAGCACG AGATCACACG CCTCACCAAC GAGAACCTGG ATCTTATGGA GCAGTTGGAG 
AAACAGGACA AGACCGTTCG TAAGCTGAAG AAACAGCTCA AAGTATTTGC TAAGAAGATC 
AATGATCTGG AAGGGGGGCA GATGGACGTG TCTCCCGGAC AAACGGCTGA CGAGCCTGTT 
CATCCGGTGA ACATTCCACG CAGAGAGAAA GATTTCCAGG GCATGCTGGA ATACAAGAAA 
GAGGATGAAC TCAAACTGGT CAAAAATATC ATACTTGAGC TGAAGCCTCG TGGTGTTGCA 
GTGAATCTGA TACCAGGTCT GCCCGCATAC ATCCTCTTCA TGTGTCTGCG CCATGCTGAC 
TACATCAACG ATGACCAGAA AGTGCGATCT CTACTCACCT CCGTCATCAA CAGCATCAAG 
AAGATCCTCA AGAAACGAGG TGATGACTTT GAGACTGTTT CCTTCTGGTT GGCCAACACA 
TGTCGTTTTC TGCACTGCCT GAAACAGTAC AGTGGTGATG AGCAATTCAT GAAGCATAAT 
AGCCCAAAGC AGAACGAACA CTGTCTCTCT AACTTTGACC TGGCAGAGTA CCGGCAGGTT 
CTGAGCGATC TGGCCATTCA GATCTACCAG CAGCTCATCA AGTGTATGGA AAACATCCTG 
CAGCCCATGA TCGTCTCTGG CATGCTGGAA CATGAAACCA TCCAGGGTGT GTCAGGAGTG 
AAGCCCACAG GTCTCCGCAA GAGGACGTCA AGTATTGCTG ATGAGGGCAC GTACACACTG 
GACTCCATCA TCCGTCAGCT CAACACCTTC CACTCCATCA TGTGTCACCA CGGCACTGAC 
CCCGAGCTCA TCAAACAGGT GGTCAAGCAG CAGTTCTACA TCATCGGCGC TGTCACCCTC 
AACAACCTGC TGCTGCGCAA AGACATGTGC TCCTGGAGCA AGGGCATGCA GATCAGGTAT 
AACGTGAGTC AGCTGGAGGA ATGGCTGAGG GATAAGAACC TCATGACATG CGGCGCTAAA 
GAAACACTGG AGCCGTTGAT TCAGGCTGCG CAACTACTGC AGGTGAAGAA GAAAACTGAT 
GAGGACGCCG AGGCCATCTG CTCTATGTGT AACGCCCTCT CCACAGCACA GATTGTGAAA 
GTGCTGAACT TGTACACACC TGTCAATGCA TTTGAAGAGC GAGTGTCAGT GTTGTTCATC 
AGAACAATAC AGACTCGTTT ACGGGATCGA AAGGAGTCGC CTCAGCTTCT GATGGACACC 
AAACTCATCT ACCCCGTGAC CTTCCCCTTT AACCCCTCCT CCCTTGCCCT GGAAACCATT 
CAGATCCCCA GCAGCCTCAA TCTGGCCTTC CTCACTCGTG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_017352118.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207607.1 Danio rerio myosin VAa (myo5aa), transcript variant X10, mRNA.

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CloneID	ODa55977
Clone ID Related Accession (Same CDS sequence)	XM_017352118.2 , XM_017352118.1
Accession Version	XM_017352118.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	unconventional myosin-Va isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGA 
TCCCCATTCC CTGACTACCA TAAGCTGAAT GAGGATGGAG AACTGTGGCT GGTTTATGAA 
GGGTTAAAGG AAACTAACAG GCTGTTGGAG TCTCAGTTGC AGGCTCAACG TCGCTCTCAT 
GACAACGAGG TGGAAGTTTT GCGTGGAGAG CTGCACAGTC TTAAGGAGGA GAACAACCGT 
CAGCAGCAGC TGTTGGCGCA GAACCTGCAA CTACCTCCAG AGGCCAGGAT TGAAGCCAGT 
CTACAGCACG AGATCACACG CCTCACCAAC GAGAACCTGG ATCTTATGGA GCAGTTGGAG 
AAACAGGACA AGACCGTTCG TAAGCTGAAG AAACAGCTCA AAGTATTTGC TAAGAAGATC 
AATGATCTGG AAGGGGGGCA GATGGACGTG TCTCCCGGAC AAACGGCTGA CGAGCCTGTT 
CATCCGGTGA ACATTCCACG CAGAGAGAAA GATTTCCAGG GCATGCTGGA ATACAAGAAA 
GAGGATGAAC TCAAACTGGT CAAAAATATC ATACTTGAGC TGAAGCCTCG TGGTGTTGCA 
GTGAATCTGA TACCAGGTCT GCCCGCATAC ATCCTCTTCA TGTGTCTGCG CCATGCTGAC 
TACATCAACG ATGACCAGAA AGTGCGATCT CTACTCACCT CCGTCATCAA CAGCATCAAG 
AAGATCCTCA AGAAACGAGG TGATGACTTT GAGACTGTTT CCTTCTGGTT GGCCAACACA 
TGTCGTTTTC TGCACTGCCT GAAACAGTAC AGTGGTGATG AGCAATTCAT GAAGCATAAT 
AGCCCAAAGC AGAACGAACA CTGTCTCTCT AACTTTGACC TGGCAGAGTA CCGGCAGGTT 
CTGAGCGATC TGGCCATTCA GATCTACCAG CAGCTCATCA AGTGTATGGA AAACATCCTG 
CAGCCCATGA TCGTCTCTGG CATGCTGGAA CATGAAACCA TCCAGGGTGT GTCAGGAGTG 
AAGCCCACAG GTCTCCGCAA GAGGACGTCA AGTATTGCTG ATGAGGGCAC GTACACACTG 
GACTCCATCA TCCGTCAGCT CAACACCTTC CACTCCATCA TGTGTCACCA CGGCACTGAC 
CCCGAGCTCA TCAAACAGGT GGTCAAGCAG CAGTTCTACA TCATCGGCGC TGTCACCCTC 
AACAACCTGC TGCTGCGCAA AGACATGTGC TCCTGGAGCA AGGGCATGCA GATCAGGTAT 
AACGTGAGTC AGCTGGAGGA ATGGCTGAGG GATAAGAACC TCATGACATG CGGCGCTAAA 
GAAACACTGG AGCCGTTGAT TCAGGCTGCG CAACTACTGC AGGTGAAGAA GAAAACTGAT 
GAGGACGCCG AGGCCATCTG CTCTATGTGT AACGCCCTCT CCACAGCACA GATTGTGAAA 
GTGCTGAACT TGTACACACC TGTCAATGCA TTTGAAGAGC GAGTGTCAGT GTTGTTCATC 
AGAACAATAC AGACTCGTTT ACGGGATCGA AAGGAGTCGC CTCAGCTTCT GATGGACACC 
AAACTCATCT ACCCCGTGAC CTTCCCCTTT AACCCCTCCT CCCTTGCCCT GGAAACCATT 
CAGATCCCCA GCAGCCTCAA TCTGGCCTTC CTCACTCGTG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207607.1
CDS	170..5734
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRSPFPDYHKLNEDGELWLVYEGLKETNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_017352118.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352118.1

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGA 
TCCCCATTCC CTGACTACCA TAAGCTGAAT GAGGATGGAG AACTGTGGCT GGTTTATGAA 
GGGTTAAAGG AAACTAACAG GCTGTTGGAG TCTCAGTTGC AGGCTCAACG TCGCTCTCAT 
GACAACGAGG TGGAAGTTTT GCGTGGAGAG CTGCACAGTC TTAAGGAGGA GAACAACCGT 
CAGCAGCAGC TGTTGGCGCA GAACCTGCAA CTACCTCCAG AGGCCAGGAT TGAAGCCAGT 
CTACAGCACG AGATCACACG CCTCACCAAC GAGAACCTGG ATCTTATGGA GCAGTTGGAG 
AAACAGGACA AGACCGTTCG TAAGCTGAAG AAACAGCTCA AAGTATTTGC TAAGAAGATC 
AATGATCTGG AAGGGGGGCA GATGGACGTG TCTCCCGGAC AAACGGCTGA CGAGCCTGTT 
CATCCGGTGA ACATTCCACG CAGAGAGAAA GATTTCCAGG GCATGCTGGA ATACAAGAAA 
GAGGATGAAC TCAAACTGGT CAAAAATATC ATACTTGAGC TGAAGCCTCG TGGTGTTGCA 
GTGAATCTGA TACCAGGTCT GCCCGCATAC ATCCTCTTCA TGTGTCTGCG CCATGCTGAC 
TACATCAACG ATGACCAGAA AGTGCGATCT CTACTCACCT CCGTCATCAA CAGCATCAAG 
AAGATCCTCA AGAAACGAGG TGATGACTTT GAGACTGTTT CCTTCTGGTT GGCCAACACA 
TGTCGTTTTC TGCACTGCCT GAAACAGTAC AGTGGTGATG AGCAATTCAT GAAGCATAAT 
AGCCCAAAGC AGAACGAACA CTGTCTCTCT AACTTTGACC TGGCAGAGTA CCGGCAGGTT 
CTGAGCGATC TGGCCATTCA GATCTACCAG CAGCTCATCA AGTGTATGGA AAACATCCTG 
CAGCCCATGA TCGTCTCTGG CATGCTGGAA CATGAAACCA TCCAGGGTGT GTCAGGAGTG 
AAGCCCACAG GTCTCCGCAA GAGGACGTCA AGTATTGCTG ATGAGGGCAC GTACACACTG 
GACTCCATCA TCCGTCAGCT CAACACCTTC CACTCCATCA TGTGTCACCA CGGCACTGAC 
CCCGAGCTCA TCAAACAGGT GGTCAAGCAG CAGTTCTACA TCATCGGCGC TGTCACCCTC 
AACAACCTGC TGCTGCGCAA AGACATGTGC TCCTGGAGCA AGGGCATGCA GATCAGGTAT 
AACGTGAGTC AGCTGGAGGA ATGGCTGAGG GATAAGAACC TCATGACATG CGGCGCTAAA 
GAAACACTGG AGCCGTTGAT TCAGGCTGCG CAACTACTGC AGGTGAAGAA GAAAACTGAT 
GAGGACGCCG AGGCCATCTG CTCTATGTGT AACGCCCTCT CCACAGCACA GATTGTGAAA 
GTGCTGAACT TGTACACACC TGTCAATGCA TTTGAAGAGC GAGTGTCAGT GTTGTTCATC 
AGAACAATAC AGACTCGTTT ACGGGATCGA AAGGAGTCGC CTCAGCTTCT GATGGACACC 
AAACTCATCT ACCCCGTGAC CTTCCCCTTT AACCCCTCCT CCCTTGCCCT GGAAACCATT 
CAGATCCCCA GCAGCCTCAA TCTGGCCTTC CTCACTCGTG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_005173675.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173732.1 Danio rerio myosin VAa (myo5aa), transcript variant X6, mRNA.

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CloneID	ODa23511
Clone ID Related Accession (Same CDS sequence)	XM_005173675.4 , XM_005173675.3
Accession Version	XM_005173675.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173675.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGGCTGTTGG AGTCTCAGTT GCAGGCTCAA CGTCGCTCTC ATGACAACGA GGTGGAAGTT 
TTGCGTGGAG AGCTGCACAG TCTTAAGGAG GAGAACAACC GTCAGCAGCA GCTGTTGGCG 
CAGAACCTGC AACTACCTCC AGAGGCCAGG ATTGAAGCCA GTCTACAGCA CGAGATCACA 
CGCCTCACCA ACGAGAACCT GGATCTTATG GAGCAGTTGG AGAAACAGGA CAAGACCGTT 
CGTAAGCTGA AGAAACAGCT CAAAGTATTT GCTAAGAAGA TCAATGATCT GGAAGGGGGG 
CAGATGGACG TGTCTCCCGG ACAAACGGCT GACGAGCCTG TTCATCCGGT GAACATTCCA 
CGCAGAGAGA AAGATTTCCA GGGCATGCTG GAATACAAGA AAGAGGATGA ACTCAAACTG 
GTCAAAAATA TCATACTTGA GCTGAAGCCT CGTGGTGTTG CAGTGAATCT GATACCAGGT 
CTGCCCGCAT ACATCCTCTT CATGTGTCTG CGCCATGCTG ACTACATCAA CGATGACCAG 
AAAGTGCGAT CTCTACTCAC CTCCGTCATC AACAGCATCA AGAAGATCCT CAAGAAACGA 
GGTGATGACT TTGAGACTGT TTCCTTCTGG TTGGCCAACA CATGTCGTTT TCTGCACTGC 
CTGAAACAGT ACAGTGGTGA TGAGCAATTC ATGAAGCATA ATAGCCCAAA GCAGAACGAA 
CACTGTCTCT CTAACTTTGA CCTGGCAGAG TACCGGCAGG TTCTGAGCGA TCTGGCCATT 
CAGATCTACC AGCAGCTCAT CAAGTGTATG GAAAACATCC TGCAGCCCAT GATCGTCTCT 
GGCATGCTGG AACATGAAAC CATCCAGGGT GTGTCAGGAG TGAAGCCCAC AGGTCTCCGC 
AAGAGGACGT CAAGTATTGC TGATGAGGGC ACGTACACAC TGGACTCCAT CATCCGTCAG 
CTCAACACCT TCCACTCCAT CATGTGTCAC CACGGCACTG ACCCCGAGCT CATCAAACAG 
GTGGTCAAGC AGCAGTTCTA CATCATCGGC GCTGTCACCC TCAACAACCT GCTGCTGCGC 
AAAGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ATAACGTGAG TCAGCTGGAG 
GAATGGCTGA GGGATAAGAA CCTCATGACA TGCGGCGCTA AAGAAACACT GGAGCCGTTG 
ATTCAGGCTG CGCAACTACT GCAGGTGAAG AAGAAAACTG ATGAGGACGC CGAGGCCATC 
TGCTCTATGT GTAACGCCCT CTCCACAGCA CAGATTGTGA AAGTGCTGAA CTTGTACACA 
CCTGTCAATG CATTTGAAGA GCGAGTGTCA GTGTTGTTCA TCAGAACAAT ACAGACTCGT 
TTACGGGATC GAAAGGAGTC GCCTCAGCTT CTGATGGACA CCAAACTCAT CTACCCCGTG 
ACCTTCCCCT TTAACCCCTC CTCCCTTGCC CTGGAAACCA TTCAGATCCC CAGCAGCCTC 
AATCTGGCCT TCCTCACTCG TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173732.1
CDS	164..5650
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_005173675.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173675.4

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGGCTGTTGG AGTCTCAGTT GCAGGCTCAA CGTCGCTCTC ATGACAACGA GGTGGAAGTT 
TTGCGTGGAG AGCTGCACAG TCTTAAGGAG GAGAACAACC GTCAGCAGCA GCTGTTGGCG 
CAGAACCTGC AACTACCTCC AGAGGCCAGG ATTGAAGCCA GTCTACAGCA CGAGATCACA 
CGCCTCACCA ACGAGAACCT GGATCTTATG GAGCAGTTGG AGAAACAGGA CAAGACCGTT 
CGTAAGCTGA AGAAACAGCT CAAAGTATTT GCTAAGAAGA TCAATGATCT GGAAGGGGGG 
CAGATGGACG TGTCTCCCGG ACAAACGGCT GACGAGCCTG TTCATCCGGT GAACATTCCA 
CGCAGAGAGA AAGATTTCCA GGGCATGCTG GAATACAAGA AAGAGGATGA ACTCAAACTG 
GTCAAAAATA TCATACTTGA GCTGAAGCCT CGTGGTGTTG CAGTGAATCT GATACCAGGT 
CTGCCCGCAT ACATCCTCTT CATGTGTCTG CGCCATGCTG ACTACATCAA CGATGACCAG 
AAAGTGCGAT CTCTACTCAC CTCCGTCATC AACAGCATCA AGAAGATCCT CAAGAAACGA 
GGTGATGACT TTGAGACTGT TTCCTTCTGG TTGGCCAACA CATGTCGTTT TCTGCACTGC 
CTGAAACAGT ACAGTGGTGA TGAGCAATTC ATGAAGCATA ATAGCCCAAA GCAGAACGAA 
CACTGTCTCT CTAACTTTGA CCTGGCAGAG TACCGGCAGG TTCTGAGCGA TCTGGCCATT 
CAGATCTACC AGCAGCTCAT CAAGTGTATG GAAAACATCC TGCAGCCCAT GATCGTCTCT 
GGCATGCTGG AACATGAAAC CATCCAGGGT GTGTCAGGAG TGAAGCCCAC AGGTCTCCGC 
AAGAGGACGT CAAGTATTGC TGATGAGGGC ACGTACACAC TGGACTCCAT CATCCGTCAG 
CTCAACACCT TCCACTCCAT CATGTGTCAC CACGGCACTG ACCCCGAGCT CATCAAACAG 
GTGGTCAAGC AGCAGTTCTA CATCATCGGC GCTGTCACCC TCAACAACCT GCTGCTGCGC 
AAAGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ATAACGTGAG TCAGCTGGAG 
GAATGGCTGA GGGATAAGAA CCTCATGACA TGCGGCGCTA AAGAAACACT GGAGCCGTTG 
ATTCAGGCTG CGCAACTACT GCAGGTGAAG AAGAAAACTG ATGAGGACGC CGAGGCCATC 
TGCTCTATGT GTAACGCCCT CTCCACAGCA CAGATTGTGA AAGTGCTGAA CTTGTACACA 
CCTGTCAATG CATTTGAAGA GCGAGTGTCA GTGTTGTTCA TCAGAACAAT ACAGACTCGT 
TTACGGGATC GAAAGGAGTC GCCTCAGCTT CTGATGGACA CCAAACTCAT CTACCCCGTG 
ACCTTCCCCT TTAACCCCTC CTCCCTTGCC CTGGAAACCA TTCAGATCCC CAGCAGCCTC 
AATCTGGCCT TCCTCACTCG TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_005173675.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173732.1 Danio rerio myosin VAa (myo5aa), transcript variant X7, mRNA.

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CloneID	ODa23511
Clone ID Related Accession (Same CDS sequence)	XM_005173675.4 , XM_005173675.3
Accession Version	XM_005173675.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	unconventional myosin-Va isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173675.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGGCTGTTGG AGTCTCAGTT GCAGGCTCAA CGTCGCTCTC ATGACAACGA GGTGGAAGTT 
TTGCGTGGAG AGCTGCACAG TCTTAAGGAG GAGAACAACC GTCAGCAGCA GCTGTTGGCG 
CAGAACCTGC AACTACCTCC AGAGGCCAGG ATTGAAGCCA GTCTACAGCA CGAGATCACA 
CGCCTCACCA ACGAGAACCT GGATCTTATG GAGCAGTTGG AGAAACAGGA CAAGACCGTT 
CGTAAGCTGA AGAAACAGCT CAAAGTATTT GCTAAGAAGA TCAATGATCT GGAAGGGGGG 
CAGATGGACG TGTCTCCCGG ACAAACGGCT GACGAGCCTG TTCATCCGGT GAACATTCCA 
CGCAGAGAGA AAGATTTCCA GGGCATGCTG GAATACAAGA AAGAGGATGA ACTCAAACTG 
GTCAAAAATA TCATACTTGA GCTGAAGCCT CGTGGTGTTG CAGTGAATCT GATACCAGGT 
CTGCCCGCAT ACATCCTCTT CATGTGTCTG CGCCATGCTG ACTACATCAA CGATGACCAG 
AAAGTGCGAT CTCTACTCAC CTCCGTCATC AACAGCATCA AGAAGATCCT CAAGAAACGA 
GGTGATGACT TTGAGACTGT TTCCTTCTGG TTGGCCAACA CATGTCGTTT TCTGCACTGC 
CTGAAACAGT ACAGTGGTGA TGAGCAATTC ATGAAGCATA ATAGCCCAAA GCAGAACGAA 
CACTGTCTCT CTAACTTTGA CCTGGCAGAG TACCGGCAGG TTCTGAGCGA TCTGGCCATT 
CAGATCTACC AGCAGCTCAT CAAGTGTATG GAAAACATCC TGCAGCCCAT GATCGTCTCT 
GGCATGCTGG AACATGAAAC CATCCAGGGT GTGTCAGGAG TGAAGCCCAC AGGTCTCCGC 
AAGAGGACGT CAAGTATTGC TGATGAGGGC ACGTACACAC TGGACTCCAT CATCCGTCAG 
CTCAACACCT TCCACTCCAT CATGTGTCAC CACGGCACTG ACCCCGAGCT CATCAAACAG 
GTGGTCAAGC AGCAGTTCTA CATCATCGGC GCTGTCACCC TCAACAACCT GCTGCTGCGC 
AAAGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ATAACGTGAG TCAGCTGGAG 
GAATGGCTGA GGGATAAGAA CCTCATGACA TGCGGCGCTA AAGAAACACT GGAGCCGTTG 
ATTCAGGCTG CGCAACTACT GCAGGTGAAG AAGAAAACTG ATGAGGACGC CGAGGCCATC 
TGCTCTATGT GTAACGCCCT CTCCACAGCA CAGATTGTGA AAGTGCTGAA CTTGTACACA 
CCTGTCAATG CATTTGAAGA GCGAGTGTCA GTGTTGTTCA TCAGAACAAT ACAGACTCGT 
TTACGGGATC GAAAGGAGTC GCCTCAGCTT CTGATGGACA CCAAACTCAT CTACCCCGTG 
ACCTTCCCCT TTAACCCCTC CTCCCTTGCC CTGGAAACCA TTCAGATCCC CAGCAGCCTC 
AATCTGGCCT TCCTCACTCG TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173732.1
CDS	172..5658
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_005173675.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173675.3

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGGCTGTTGG AGTCTCAGTT GCAGGCTCAA CGTCGCTCTC ATGACAACGA GGTGGAAGTT 
TTGCGTGGAG AGCTGCACAG TCTTAAGGAG GAGAACAACC GTCAGCAGCA GCTGTTGGCG 
CAGAACCTGC AACTACCTCC AGAGGCCAGG ATTGAAGCCA GTCTACAGCA CGAGATCACA 
CGCCTCACCA ACGAGAACCT GGATCTTATG GAGCAGTTGG AGAAACAGGA CAAGACCGTT 
CGTAAGCTGA AGAAACAGCT CAAAGTATTT GCTAAGAAGA TCAATGATCT GGAAGGGGGG 
CAGATGGACG TGTCTCCCGG ACAAACGGCT GACGAGCCTG TTCATCCGGT GAACATTCCA 
CGCAGAGAGA AAGATTTCCA GGGCATGCTG GAATACAAGA AAGAGGATGA ACTCAAACTG 
GTCAAAAATA TCATACTTGA GCTGAAGCCT CGTGGTGTTG CAGTGAATCT GATACCAGGT 
CTGCCCGCAT ACATCCTCTT CATGTGTCTG CGCCATGCTG ACTACATCAA CGATGACCAG 
AAAGTGCGAT CTCTACTCAC CTCCGTCATC AACAGCATCA AGAAGATCCT CAAGAAACGA 
GGTGATGACT TTGAGACTGT TTCCTTCTGG TTGGCCAACA CATGTCGTTT TCTGCACTGC 
CTGAAACAGT ACAGTGGTGA TGAGCAATTC ATGAAGCATA ATAGCCCAAA GCAGAACGAA 
CACTGTCTCT CTAACTTTGA CCTGGCAGAG TACCGGCAGG TTCTGAGCGA TCTGGCCATT 
CAGATCTACC AGCAGCTCAT CAAGTGTATG GAAAACATCC TGCAGCCCAT GATCGTCTCT 
GGCATGCTGG AACATGAAAC CATCCAGGGT GTGTCAGGAG TGAAGCCCAC AGGTCTCCGC 
AAGAGGACGT CAAGTATTGC TGATGAGGGC ACGTACACAC TGGACTCCAT CATCCGTCAG 
CTCAACACCT TCCACTCCAT CATGTGTCAC CACGGCACTG ACCCCGAGCT CATCAAACAG 
GTGGTCAAGC AGCAGTTCTA CATCATCGGC GCTGTCACCC TCAACAACCT GCTGCTGCGC 
AAAGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ATAACGTGAG TCAGCTGGAG 
GAATGGCTGA GGGATAAGAA CCTCATGACA TGCGGCGCTA AAGAAACACT GGAGCCGTTG 
ATTCAGGCTG CGCAACTACT GCAGGTGAAG AAGAAAACTG ATGAGGACGC CGAGGCCATC 
TGCTCTATGT GTAACGCCCT CTCCACAGCA CAGATTGTGA AAGTGCTGAA CTTGTACACA 
CCTGTCAATG CATTTGAAGA GCGAGTGTCA GTGTTGTTCA TCAGAACAAT ACAGACTCGT 
TTACGGGATC GAAAGGAGTC GCCTCAGCTT CTGATGGACA CCAAACTCAT CTACCCCGTG 
ACCTTCCCCT TTAACCCCTC CTCCCTTGCC CTGGAAACCA TTCAGATCCC CAGCAGCCTC 
AATCTGGCCT TCCTCACTCG TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_005173676.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173733.1 Danio rerio myosin VAa (myo5aa), transcript variant X8, mRNA.

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CloneID	ODa23512
Clone ID Related Accession (Same CDS sequence)	XM_005173676.3 , XM_005173676.4
Accession Version	XM_005173676.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	unconventional myosin-Va isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173676.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGG 
CTGTTGGAGT CTCAGTTGCA GGCTCAACGT CGCTCTCATG ACAACGAGGT GGAAGTTTTG 
CGTGGAGAGC TGCACAGTCT TAAGGAGGAG AACAACCGTC AGCAGCAGCT GTTGGCGCAG 
AACCTGCAAC TACCTCCAGA GGCCAGGATT GAAGCCAGTC TACAGCACGA GATCACACGC 
CTCACCAACG AGAACCTGGA TCTTATGGAG CAGTTGGAGA AACAGGACAA GACCGTTCGT 
AAGCTGAAGA AACAGCTCAA AGTATTTGCT AAGAAGATCA ATGATCTGGA AGGGGGGCAG 
ATGGACGTGT CTCCCGGACA AACGGCTGAC GAGCCTGTTC ATCCGGTGAA CATTCCACGC 
AGAGAGAAAG ATTTCCAGGG CATGCTGGAA TACAAGAAAG AGGATGAACT CAAACTGGTC 
AAAAATATCA TACTTGAGCT GAAGCCTCGT GGTGTTGCAG TGAATCTGAT ACCAGGTCTG 
CCCGCATACA TCCTCTTCAT GTGTCTGCGC CATGCTGACT ACATCAACGA TGACCAGAAA 
GTGCGATCTC TACTCACCTC CGTCATCAAC AGCATCAAGA AGATCCTCAA GAAACGAGGT 
GATGACTTTG AGACTGTTTC CTTCTGGTTG GCCAACACAT GTCGTTTTCT GCACTGCCTG 
AAACAGTACA GTGGTGATGA GCAATTCATG AAGCATAATA GCCCAAAGCA GAACGAACAC 
TGTCTCTCTA ACTTTGACCT GGCAGAGTAC CGGCAGGTTC TGAGCGATCT GGCCATTCAG 
ATCTACCAGC AGCTCATCAA GTGTATGGAA AACATCCTGC AGCCCATGAT CGTCTCTGGC 
ATGCTGGAAC ATGAAACCAT CCAGGGTGTG TCAGGAGTGA AGCCCACAGG TCTCCGCAAG 
AGGACGTCAA GTATTGCTGA TGAGGGCACG TACACACTGG ACTCCATCAT CCGTCAGCTC 
AACACCTTCC ACTCCATCAT GTGTCACCAC GGCACTGACC CCGAGCTCAT CAAACAGGTG 
GTCAAGCAGC AGTTCTACAT CATCGGCGCT GTCACCCTCA ACAACCTGCT GCTGCGCAAA 
GACATGTGCT CCTGGAGCAA GGGCATGCAG ATCAGGTATA ACGTGAGTCA GCTGGAGGAA 
TGGCTGAGGG ATAAGAACCT CATGACATGC GGCGCTAAAG AAACACTGGA GCCGTTGATT 
CAGGCTGCGC AACTACTGCA GGTGAAGAAG AAAACTGATG AGGACGCCGA GGCCATCTGC 
TCTATGTGTA ACGCCCTCTC CACAGCACAG ATTGTGAAAG TGCTGAACTT GTACACACCT 
GTCAATGCAT TTGAAGAGCG AGTGTCAGTG TTGTTCATCA GAACAATACA GACTCGTTTA 
CGGGATCGAA AGGAGTCGCC TCAGCTTCTG ATGGACACCA AACTCATCTA CCCCGTGACC 
TTCCCCTTTA ACCCCTCCTC CCTTGCCCTG GAAACCATTC AGATCCCCAG CAGCCTCAAT 
CTGGCCTTCC TCACTCGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173733.1
CDS	172..5655
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_005173676.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173676.3

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGG 
CTGTTGGAGT CTCAGTTGCA GGCTCAACGT CGCTCTCATG ACAACGAGGT GGAAGTTTTG 
CGTGGAGAGC TGCACAGTCT TAAGGAGGAG AACAACCGTC AGCAGCAGCT GTTGGCGCAG 
AACCTGCAAC TACCTCCAGA GGCCAGGATT GAAGCCAGTC TACAGCACGA GATCACACGC 
CTCACCAACG AGAACCTGGA TCTTATGGAG CAGTTGGAGA AACAGGACAA GACCGTTCGT 
AAGCTGAAGA AACAGCTCAA AGTATTTGCT AAGAAGATCA ATGATCTGGA AGGGGGGCAG 
ATGGACGTGT CTCCCGGACA AACGGCTGAC GAGCCTGTTC ATCCGGTGAA CATTCCACGC 
AGAGAGAAAG ATTTCCAGGG CATGCTGGAA TACAAGAAAG AGGATGAACT CAAACTGGTC 
AAAAATATCA TACTTGAGCT GAAGCCTCGT GGTGTTGCAG TGAATCTGAT ACCAGGTCTG 
CCCGCATACA TCCTCTTCAT GTGTCTGCGC CATGCTGACT ACATCAACGA TGACCAGAAA 
GTGCGATCTC TACTCACCTC CGTCATCAAC AGCATCAAGA AGATCCTCAA GAAACGAGGT 
GATGACTTTG AGACTGTTTC CTTCTGGTTG GCCAACACAT GTCGTTTTCT GCACTGCCTG 
AAACAGTACA GTGGTGATGA GCAATTCATG AAGCATAATA GCCCAAAGCA GAACGAACAC 
TGTCTCTCTA ACTTTGACCT GGCAGAGTAC CGGCAGGTTC TGAGCGATCT GGCCATTCAG 
ATCTACCAGC AGCTCATCAA GTGTATGGAA AACATCCTGC AGCCCATGAT CGTCTCTGGC 
ATGCTGGAAC ATGAAACCAT CCAGGGTGTG TCAGGAGTGA AGCCCACAGG TCTCCGCAAG 
AGGACGTCAA GTATTGCTGA TGAGGGCACG TACACACTGG ACTCCATCAT CCGTCAGCTC 
AACACCTTCC ACTCCATCAT GTGTCACCAC GGCACTGACC CCGAGCTCAT CAAACAGGTG 
GTCAAGCAGC AGTTCTACAT CATCGGCGCT GTCACCCTCA ACAACCTGCT GCTGCGCAAA 
GACATGTGCT CCTGGAGCAA GGGCATGCAG ATCAGGTATA ACGTGAGTCA GCTGGAGGAA 
TGGCTGAGGG ATAAGAACCT CATGACATGC GGCGCTAAAG AAACACTGGA GCCGTTGATT 
CAGGCTGCGC AACTACTGCA GGTGAAGAAG AAAACTGATG AGGACGCCGA GGCCATCTGC 
TCTATGTGTA ACGCCCTCTC CACAGCACAG ATTGTGAAAG TGCTGAACTT GTACACACCT 
GTCAATGCAT TTGAAGAGCG AGTGTCAGTG TTGTTCATCA GAACAATACA GACTCGTTTA 
CGGGATCGAA AGGAGTCGCC TCAGCTTCTG ATGGACACCA AACTCATCTA CCCCGTGACC 
TTCCCCTTTA ACCCCTCCTC CCTTGCCCTG GAAACCATTC AGATCCCCAG CAGCCTCAAT 
CTGGCCTTCC TCACTCGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_005173676.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173733.1 Danio rerio myosin VAa (myo5aa), transcript variant X7, mRNA.

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CloneID	ODa23512
Clone ID Related Accession (Same CDS sequence)	XM_005173676.3 , XM_005173676.4
Accession Version	XM_005173676.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173676.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGG 
CTGTTGGAGT CTCAGTTGCA GGCTCAACGT CGCTCTCATG ACAACGAGGT GGAAGTTTTG 
CGTGGAGAGC TGCACAGTCT TAAGGAGGAG AACAACCGTC AGCAGCAGCT GTTGGCGCAG 
AACCTGCAAC TACCTCCAGA GGCCAGGATT GAAGCCAGTC TACAGCACGA GATCACACGC 
CTCACCAACG AGAACCTGGA TCTTATGGAG CAGTTGGAGA AACAGGACAA GACCGTTCGT 
AAGCTGAAGA AACAGCTCAA AGTATTTGCT AAGAAGATCA ATGATCTGGA AGGGGGGCAG 
ATGGACGTGT CTCCCGGACA AACGGCTGAC GAGCCTGTTC ATCCGGTGAA CATTCCACGC 
AGAGAGAAAG ATTTCCAGGG CATGCTGGAA TACAAGAAAG AGGATGAACT CAAACTGGTC 
AAAAATATCA TACTTGAGCT GAAGCCTCGT GGTGTTGCAG TGAATCTGAT ACCAGGTCTG 
CCCGCATACA TCCTCTTCAT GTGTCTGCGC CATGCTGACT ACATCAACGA TGACCAGAAA 
GTGCGATCTC TACTCACCTC CGTCATCAAC AGCATCAAGA AGATCCTCAA GAAACGAGGT 
GATGACTTTG AGACTGTTTC CTTCTGGTTG GCCAACACAT GTCGTTTTCT GCACTGCCTG 
AAACAGTACA GTGGTGATGA GCAATTCATG AAGCATAATA GCCCAAAGCA GAACGAACAC 
TGTCTCTCTA ACTTTGACCT GGCAGAGTAC CGGCAGGTTC TGAGCGATCT GGCCATTCAG 
ATCTACCAGC AGCTCATCAA GTGTATGGAA AACATCCTGC AGCCCATGAT CGTCTCTGGC 
ATGCTGGAAC ATGAAACCAT CCAGGGTGTG TCAGGAGTGA AGCCCACAGG TCTCCGCAAG 
AGGACGTCAA GTATTGCTGA TGAGGGCACG TACACACTGG ACTCCATCAT CCGTCAGCTC 
AACACCTTCC ACTCCATCAT GTGTCACCAC GGCACTGACC CCGAGCTCAT CAAACAGGTG 
GTCAAGCAGC AGTTCTACAT CATCGGCGCT GTCACCCTCA ACAACCTGCT GCTGCGCAAA 
GACATGTGCT CCTGGAGCAA GGGCATGCAG ATCAGGTATA ACGTGAGTCA GCTGGAGGAA 
TGGCTGAGGG ATAAGAACCT CATGACATGC GGCGCTAAAG AAACACTGGA GCCGTTGATT 
CAGGCTGCGC AACTACTGCA GGTGAAGAAG AAAACTGATG AGGACGCCGA GGCCATCTGC 
TCTATGTGTA ACGCCCTCTC CACAGCACAG ATTGTGAAAG TGCTGAACTT GTACACACCT 
GTCAATGCAT TTGAAGAGCG AGTGTCAGTG TTGTTCATCA GAACAATACA GACTCGTTTA 
CGGGATCGAA AGGAGTCGCC TCAGCTTCTG ATGGACACCA AACTCATCTA CCCCGTGACC 
TTCCCCTTTA ACCCCTCCTC CCTTGCCCTG GAAACCATTC AGATCCCCAG CAGCCTCAAT 
CTGGCCTTCC TCACTCGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173733.1
CDS	164..5647
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_005173676.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173676.4

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGG 
CTGTTGGAGT CTCAGTTGCA GGCTCAACGT CGCTCTCATG ACAACGAGGT GGAAGTTTTG 
CGTGGAGAGC TGCACAGTCT TAAGGAGGAG AACAACCGTC AGCAGCAGCT GTTGGCGCAG 
AACCTGCAAC TACCTCCAGA GGCCAGGATT GAAGCCAGTC TACAGCACGA GATCACACGC 
CTCACCAACG AGAACCTGGA TCTTATGGAG CAGTTGGAGA AACAGGACAA GACCGTTCGT 
AAGCTGAAGA AACAGCTCAA AGTATTTGCT AAGAAGATCA ATGATCTGGA AGGGGGGCAG 
ATGGACGTGT CTCCCGGACA AACGGCTGAC GAGCCTGTTC ATCCGGTGAA CATTCCACGC 
AGAGAGAAAG ATTTCCAGGG CATGCTGGAA TACAAGAAAG AGGATGAACT CAAACTGGTC 
AAAAATATCA TACTTGAGCT GAAGCCTCGT GGTGTTGCAG TGAATCTGAT ACCAGGTCTG 
CCCGCATACA TCCTCTTCAT GTGTCTGCGC CATGCTGACT ACATCAACGA TGACCAGAAA 
GTGCGATCTC TACTCACCTC CGTCATCAAC AGCATCAAGA AGATCCTCAA GAAACGAGGT 
GATGACTTTG AGACTGTTTC CTTCTGGTTG GCCAACACAT GTCGTTTTCT GCACTGCCTG 
AAACAGTACA GTGGTGATGA GCAATTCATG AAGCATAATA GCCCAAAGCA GAACGAACAC 
TGTCTCTCTA ACTTTGACCT GGCAGAGTAC CGGCAGGTTC TGAGCGATCT GGCCATTCAG 
ATCTACCAGC AGCTCATCAA GTGTATGGAA AACATCCTGC AGCCCATGAT CGTCTCTGGC 
ATGCTGGAAC ATGAAACCAT CCAGGGTGTG TCAGGAGTGA AGCCCACAGG TCTCCGCAAG 
AGGACGTCAA GTATTGCTGA TGAGGGCACG TACACACTGG ACTCCATCAT CCGTCAGCTC 
AACACCTTCC ACTCCATCAT GTGTCACCAC GGCACTGACC CCGAGCTCAT CAAACAGGTG 
GTCAAGCAGC AGTTCTACAT CATCGGCGCT GTCACCCTCA ACAACCTGCT GCTGCGCAAA 
GACATGTGCT CCTGGAGCAA GGGCATGCAG ATCAGGTATA ACGTGAGTCA GCTGGAGGAA 
TGGCTGAGGG ATAAGAACCT CATGACATGC GGCGCTAAAG AAACACTGGA GCCGTTGATT 
CAGGCTGCGC AACTACTGCA GGTGAAGAAG AAAACTGATG AGGACGCCGA GGCCATCTGC 
TCTATGTGTA ACGCCCTCTC CACAGCACAG ATTGTGAAAG TGCTGAACTT GTACACACCT 
GTCAATGCAT TTGAAGAGCG AGTGTCAGTG TTGTTCATCA GAACAATACA GACTCGTTTA 
CGGGATCGAA AGGAGTCGCC TCAGCTTCTG ATGGACACCA AACTCATCTA CCCCGTGACC 
TTCCCCTTTA ACCCCTCCTC CCTTGCCCTG GAAACCATTC AGATCCCCAG CAGCCTCAAT 
CTGGCCTTCC TCACTCGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293794.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292069.1 Danio rerio myosin VAa (myo5aa), transcript variant X1, mRNA.

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CloneID	ODa43730
Clone ID Related Accession (Same CDS sequence)	XM_009293794.2 , XM_009293794.3
Accession Version	XM_009293794.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	unconventional myosin-Va isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGGCTGTTGG AGTCTCAGTT GCAGGCTCAA CGTCGCTCTC ATGACAACGA GGTGGAAGTT 
TTGCGTGGAG AGCTGCACAG TCTTAAGGAG GAGAACAACC GTCAGCAGCA GCTGTTGGCG 
CAGAACCTGC AACTACCTCC AGAGGCCAGG ATTGAAGCCA GTCTACAGCA CGAGATCACA 
CGCCTCACCA ACGAGAACCT GGAACTTCAC TCTGATGATC CAAAGGAGTA TAAAGCCTAC 
AGAATCAGCA TGTTACGCAG GATGGTTGAT CTTATGGAGC AGTTGGAGAA ACAGGACAAG 
ACCGTTCGTA AGCTGAAGAA ACAGCTCAAA GTATTTGCTA AGAAGATCAA TGATCTGGAA 
GGGGGGCAGA TGGACGTGTC TCCCGGACAA ACGGCTGACG AGCCTGTTCA TCCGGTGAAC 
ATTCCACGCA GAGAGAAAGA TTTCCAGGGC ATGCTGGAAT ACAAGAAAGA GGATGAACTC 
AAACTGGTCA AAAATATCAT ACTTGAGCTG AAGCCTCGTG GTGTTGCAGT GAATCTGATA 
CCAGGTCTGC CCGCATACAT CCTCTTCATG TGTCTGCGCC ATGCTGACTA CATCAACGAT 
GACCAGAAAG TGCGATCTCT ACTCACCTCC GTCATCAACA GCATCAAGAA GATCCTCAAG 
AAACGAGGTG ATGACTTTGA GACTGTTTCC TTCTGGTTGG CCAACACATG TCGTTTTCTG 
CACTGCCTGA AACAGTACAG TGGTGATGAG CAATTCATGA AGCATAATAG CCCAAAGCAG 
AACGAACACT GTCTCTCTAA CTTTGACCTG GCAGAGTACC GGCAGGTTCT GAGCGATCTG 
GCCATTCAGA TCTACCAGCA GCTCATCAAG TGTATGGAAA ACATCCTGCA GCCCATGATC 
GTCTCTGGCA TGCTGGAACA TGAAACCATC CAGGGTGTGT CAGGAGTGAA GCCCACAGGT 
CTCCGCAAGA GGACGTCAAG TATTGCTGAT GAGGGCACGT ACACACTGGA CTCCATCATC 
CGTCAGCTCA ACACCTTCCA CTCCATCATG TGTCACCACG GCACTGACCC CGAGCTCATC 
AAACAGGTGG TCAAGCAGCA GTTCTACATC ATCGGCGCTG TCACCCTCAA CAACCTGCTG 
CTGCGCAAAG ACATGTGCTC CTGGAGCAAG GGCATGCAGA TCAGGTATAA CGTGAGTCAG 
CTGGAGGAAT GGCTGAGGGA TAAGAACCTC ATGACATGCG GCGCTAAAGA AACACTGGAG 
CCGTTGATTC AGGCTGCGCA ACTACTGCAG GTGAAGAAGA AAACTGATGA GGACGCCGAG 
GCCATCTGCT CTATGTGTAA CGCCCTCTCC ACAGCACAGA TTGTGAAAGT GCTGAACTTG 
TACACACCTG TCAATGCATT TGAAGAGCGA GTGTCAGTGT TGTTCATCAG AACAATACAG 
ACTCGTTTAC GGGATCGAAA GGAGTCGCCT CAGCTTCTGA TGGACACCAA ACTCATCTAC 
CCCGTGACCT TCCCCTTTAA CCCCTCCTCC CTTGCCCTGG AAACCATTCA GATCCCCAGC 
AGCCTCAATC TGGCCTTCCT CACTCGTGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292069.1
CDS	170..5722
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293794.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293794.2

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGGCTGTTGG AGTCTCAGTT GCAGGCTCAA CGTCGCTCTC ATGACAACGA GGTGGAAGTT 
TTGCGTGGAG AGCTGCACAG TCTTAAGGAG GAGAACAACC GTCAGCAGCA GCTGTTGGCG 
CAGAACCTGC AACTACCTCC AGAGGCCAGG ATTGAAGCCA GTCTACAGCA CGAGATCACA 
CGCCTCACCA ACGAGAACCT GGAACTTCAC TCTGATGATC CAAAGGAGTA TAAAGCCTAC 
AGAATCAGCA TGTTACGCAG GATGGTTGAT CTTATGGAGC AGTTGGAGAA ACAGGACAAG 
ACCGTTCGTA AGCTGAAGAA ACAGCTCAAA GTATTTGCTA AGAAGATCAA TGATCTGGAA 
GGGGGGCAGA TGGACGTGTC TCCCGGACAA ACGGCTGACG AGCCTGTTCA TCCGGTGAAC 
ATTCCACGCA GAGAGAAAGA TTTCCAGGGC ATGCTGGAAT ACAAGAAAGA GGATGAACTC 
AAACTGGTCA AAAATATCAT ACTTGAGCTG AAGCCTCGTG GTGTTGCAGT GAATCTGATA 
CCAGGTCTGC CCGCATACAT CCTCTTCATG TGTCTGCGCC ATGCTGACTA CATCAACGAT 
GACCAGAAAG TGCGATCTCT ACTCACCTCC GTCATCAACA GCATCAAGAA GATCCTCAAG 
AAACGAGGTG ATGACTTTGA GACTGTTTCC TTCTGGTTGG CCAACACATG TCGTTTTCTG 
CACTGCCTGA AACAGTACAG TGGTGATGAG CAATTCATGA AGCATAATAG CCCAAAGCAG 
AACGAACACT GTCTCTCTAA CTTTGACCTG GCAGAGTACC GGCAGGTTCT GAGCGATCTG 
GCCATTCAGA TCTACCAGCA GCTCATCAAG TGTATGGAAA ACATCCTGCA GCCCATGATC 
GTCTCTGGCA TGCTGGAACA TGAAACCATC CAGGGTGTGT CAGGAGTGAA GCCCACAGGT 
CTCCGCAAGA GGACGTCAAG TATTGCTGAT GAGGGCACGT ACACACTGGA CTCCATCATC 
CGTCAGCTCA ACACCTTCCA CTCCATCATG TGTCACCACG GCACTGACCC CGAGCTCATC 
AAACAGGTGG TCAAGCAGCA GTTCTACATC ATCGGCGCTG TCACCCTCAA CAACCTGCTG 
CTGCGCAAAG ACATGTGCTC CTGGAGCAAG GGCATGCAGA TCAGGTATAA CGTGAGTCAG 
CTGGAGGAAT GGCTGAGGGA TAAGAACCTC ATGACATGCG GCGCTAAAGA AACACTGGAG 
CCGTTGATTC AGGCTGCGCA ACTACTGCAG GTGAAGAAGA AAACTGATGA GGACGCCGAG 
GCCATCTGCT CTATGTGTAA CGCCCTCTCC ACAGCACAGA TTGTGAAAGT GCTGAACTTG 
TACACACCTG TCAATGCATT TGAAGAGCGA GTGTCAGTGT TGTTCATCAG AACAATACAG 
ACTCGTTTAC GGGATCGAAA GGAGTCGCCT CAGCTTCTGA TGGACACCAA ACTCATCTAC 
CCCGTGACCT TCCCCTTTAA CCCCTCCTCC CTTGCCCTGG AAACCATTCA GATCCCCAGC 
AGCCTCAATC TGGCCTTCCT CACTCGTGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293794.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292069.1 Danio rerio myosin VAa (myo5aa), transcript variant X1, mRNA.

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CloneID	ODa43730
Clone ID Related Accession (Same CDS sequence)	XM_009293794.2 , XM_009293794.3
Accession Version	XM_009293794.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293794.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGGCTGTTGG AGTCTCAGTT GCAGGCTCAA CGTCGCTCTC ATGACAACGA GGTGGAAGTT 
TTGCGTGGAG AGCTGCACAG TCTTAAGGAG GAGAACAACC GTCAGCAGCA GCTGTTGGCG 
CAGAACCTGC AACTACCTCC AGAGGCCAGG ATTGAAGCCA GTCTACAGCA CGAGATCACA 
CGCCTCACCA ACGAGAACCT GGAACTTCAC TCTGATGATC CAAAGGAGTA TAAAGCCTAC 
AGAATCAGCA TGTTACGCAG GATGGTTGAT CTTATGGAGC AGTTGGAGAA ACAGGACAAG 
ACCGTTCGTA AGCTGAAGAA ACAGCTCAAA GTATTTGCTA AGAAGATCAA TGATCTGGAA 
GGGGGGCAGA TGGACGTGTC TCCCGGACAA ACGGCTGACG AGCCTGTTCA TCCGGTGAAC 
ATTCCACGCA GAGAGAAAGA TTTCCAGGGC ATGCTGGAAT ACAAGAAAGA GGATGAACTC 
AAACTGGTCA AAAATATCAT ACTTGAGCTG AAGCCTCGTG GTGTTGCAGT GAATCTGATA 
CCAGGTCTGC CCGCATACAT CCTCTTCATG TGTCTGCGCC ATGCTGACTA CATCAACGAT 
GACCAGAAAG TGCGATCTCT ACTCACCTCC GTCATCAACA GCATCAAGAA GATCCTCAAG 
AAACGAGGTG ATGACTTTGA GACTGTTTCC TTCTGGTTGG CCAACACATG TCGTTTTCTG 
CACTGCCTGA AACAGTACAG TGGTGATGAG CAATTCATGA AGCATAATAG CCCAAAGCAG 
AACGAACACT GTCTCTCTAA CTTTGACCTG GCAGAGTACC GGCAGGTTCT GAGCGATCTG 
GCCATTCAGA TCTACCAGCA GCTCATCAAG TGTATGGAAA ACATCCTGCA GCCCATGATC 
GTCTCTGGCA TGCTGGAACA TGAAACCATC CAGGGTGTGT CAGGAGTGAA GCCCACAGGT 
CTCCGCAAGA GGACGTCAAG TATTGCTGAT GAGGGCACGT ACACACTGGA CTCCATCATC 
CGTCAGCTCA ACACCTTCCA CTCCATCATG TGTCACCACG GCACTGACCC CGAGCTCATC 
AAACAGGTGG TCAAGCAGCA GTTCTACATC ATCGGCGCTG TCACCCTCAA CAACCTGCTG 
CTGCGCAAAG ACATGTGCTC CTGGAGCAAG GGCATGCAGA TCAGGTATAA CGTGAGTCAG 
CTGGAGGAAT GGCTGAGGGA TAAGAACCTC ATGACATGCG GCGCTAAAGA AACACTGGAG 
CCGTTGATTC AGGCTGCGCA ACTACTGCAG GTGAAGAAGA AAACTGATGA GGACGCCGAG 
GCCATCTGCT CTATGTGTAA CGCCCTCTCC ACAGCACAGA TTGTGAAAGT GCTGAACTTG 
TACACACCTG TCAATGCATT TGAAGAGCGA GTGTCAGTGT TGTTCATCAG AACAATACAG 
ACTCGTTTAC GGGATCGAAA GGAGTCGCCT CAGCTTCTGA TGGACACCAA ACTCATCTAC 
CCCGTGACCT TCCCCTTTAA CCCCTCCTCC CTTGCCCTGG AAACCATTCA GATCCCCAGC 
AGCCTCAATC TGGCCTTCCT CACTCGTGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292069.1
CDS	165..5717
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293794.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293794.3

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGGATGAGAA GACGACTATG ACTGAACCTC TGCCATACAT TGAGGATGTG 
CAGAAGCTCA CTGATGCCAA GGAGATCTCA CAGGCCTACA TGGGCCTCAA AGACACCAAC 
AGGCTGTTGG AGTCTCAGTT GCAGGCTCAA CGTCGCTCTC ATGACAACGA GGTGGAAGTT 
TTGCGTGGAG AGCTGCACAG TCTTAAGGAG GAGAACAACC GTCAGCAGCA GCTGTTGGCG 
CAGAACCTGC AACTACCTCC AGAGGCCAGG ATTGAAGCCA GTCTACAGCA CGAGATCACA 
CGCCTCACCA ACGAGAACCT GGAACTTCAC TCTGATGATC CAAAGGAGTA TAAAGCCTAC 
AGAATCAGCA TGTTACGCAG GATGGTTGAT CTTATGGAGC AGTTGGAGAA ACAGGACAAG 
ACCGTTCGTA AGCTGAAGAA ACAGCTCAAA GTATTTGCTA AGAAGATCAA TGATCTGGAA 
GGGGGGCAGA TGGACGTGTC TCCCGGACAA ACGGCTGACG AGCCTGTTCA TCCGGTGAAC 
ATTCCACGCA GAGAGAAAGA TTTCCAGGGC ATGCTGGAAT ACAAGAAAGA GGATGAACTC 
AAACTGGTCA AAAATATCAT ACTTGAGCTG AAGCCTCGTG GTGTTGCAGT GAATCTGATA 
CCAGGTCTGC CCGCATACAT CCTCTTCATG TGTCTGCGCC ATGCTGACTA CATCAACGAT 
GACCAGAAAG TGCGATCTCT ACTCACCTCC GTCATCAACA GCATCAAGAA GATCCTCAAG 
AAACGAGGTG ATGACTTTGA GACTGTTTCC TTCTGGTTGG CCAACACATG TCGTTTTCTG 
CACTGCCTGA AACAGTACAG TGGTGATGAG CAATTCATGA AGCATAATAG CCCAAAGCAG 
AACGAACACT GTCTCTCTAA CTTTGACCTG GCAGAGTACC GGCAGGTTCT GAGCGATCTG 
GCCATTCAGA TCTACCAGCA GCTCATCAAG TGTATGGAAA ACATCCTGCA GCCCATGATC 
GTCTCTGGCA TGCTGGAACA TGAAACCATC CAGGGTGTGT CAGGAGTGAA GCCCACAGGT 
CTCCGCAAGA GGACGTCAAG TATTGCTGAT GAGGGCACGT ACACACTGGA CTCCATCATC 
CGTCAGCTCA ACACCTTCCA CTCCATCATG TGTCACCACG GCACTGACCC CGAGCTCATC 
AAACAGGTGG TCAAGCAGCA GTTCTACATC ATCGGCGCTG TCACCCTCAA CAACCTGCTG 
CTGCGCAAAG ACATGTGCTC CTGGAGCAAG GGCATGCAGA TCAGGTATAA CGTGAGTCAG 
CTGGAGGAAT GGCTGAGGGA TAAGAACCTC ATGACATGCG GCGCTAAAGA AACACTGGAG 
CCGTTGATTC AGGCTGCGCA ACTACTGCAG GTGAAGAAGA AAACTGATGA GGACGCCGAG 
GCCATCTGCT CTATGTGTAA CGCCCTCTCC ACAGCACAGA TTGTGAAAGT GCTGAACTTG 
TACACACCTG TCAATGCATT TGAAGAGCGA GTGTCAGTGT TGTTCATCAG AACAATACAG 
ACTCGTTTAC GGGATCGAAA GGAGTCGCCT CAGCTTCTGA TGGACACCAA ACTCATCTAC 
CCCGTGACCT TCCCCTTTAA CCCCTCCTCC CTTGCCCTGG AAACCATTCA GATCCCCAGC 
AGCCTCAATC TGGCCTTCCT CACTCGTGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293795.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292070.1 Danio rerio myosin VAa (myo5aa), transcript variant X2, mRNA.

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CloneID	ODa43731
Clone ID Related Accession (Same CDS sequence)	XM_009293795.2 , XM_009293795.3
Accession Version	XM_009293795.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	unconventional myosin-Va isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGG 
CTGTTGGAGT CTCAGTTGCA GGCTCAACGT CGCTCTCATG ACAACGAGGT GGAAGTTTTG 
CGTGGAGAGC TGCACAGTCT TAAGGAGGAG AACAACCGTC AGCAGCAGCT GTTGGCGCAG 
AACCTGCAAC TACCTCCAGA GGCCAGGATT GAAGCCAGTC TACAGCACGA GATCACACGC 
CTCACCAACG AGAACCTGGA ACTTCACTCT GATGATCCAA AGGAGTATAA AGCCTACAGA 
ATCAGCATGT TACGCAGGAT GGTTGATCTT ATGGAGCAGT TGGAGAAACA GGACAAGACC 
GTTCGTAAGC TGAAGAAACA GCTCAAAGTA TTTGCTAAGA AGATCAATGA TCTGGAAGGG 
GGGCAGATGG ACGTGTCTCC CGGACAAACG GCTGACGAGC CTGTTCATCC GGTGAACATT 
CCACGCAGAG AGAAAGATTT CCAGGGCATG CTGGAATACA AGAAAGAGGA TGAACTCAAA 
CTGGTCAAAA ATATCATACT TGAGCTGAAG CCTCGTGGTG TTGCAGTGAA TCTGATACCA 
GGTCTGCCCG CATACATCCT CTTCATGTGT CTGCGCCATG CTGACTACAT CAACGATGAC 
CAGAAAGTGC GATCTCTACT CACCTCCGTC ATCAACAGCA TCAAGAAGAT CCTCAAGAAA 
CGAGGTGATG ACTTTGAGAC TGTTTCCTTC TGGTTGGCCA ACACATGTCG TTTTCTGCAC 
TGCCTGAAAC AGTACAGTGG TGATGAGCAA TTCATGAAGC ATAATAGCCC AAAGCAGAAC 
GAACACTGTC TCTCTAACTT TGACCTGGCA GAGTACCGGC AGGTTCTGAG CGATCTGGCC 
ATTCAGATCT ACCAGCAGCT CATCAAGTGT ATGGAAAACA TCCTGCAGCC CATGATCGTC 
TCTGGCATGC TGGAACATGA AACCATCCAG GGTGTGTCAG GAGTGAAGCC CACAGGTCTC 
CGCAAGAGGA CGTCAAGTAT TGCTGATGAG GGCACGTACA CACTGGACTC CATCATCCGT 
CAGCTCAACA CCTTCCACTC CATCATGTGT CACCACGGCA CTGACCCCGA GCTCATCAAA 
CAGGTGGTCA AGCAGCAGTT CTACATCATC GGCGCTGTCA CCCTCAACAA CCTGCTGCTG 
CGCAAAGACA TGTGCTCCTG GAGCAAGGGC ATGCAGATCA GGTATAACGT GAGTCAGCTG 
GAGGAATGGC TGAGGGATAA GAACCTCATG ACATGCGGCG CTAAAGAAAC ACTGGAGCCG 
TTGATTCAGG CTGCGCAACT ACTGCAGGTG AAGAAGAAAA CTGATGAGGA CGCCGAGGCC 
ATCTGCTCTA TGTGTAACGC CCTCTCCACA GCACAGATTG TGAAAGTGCT GAACTTGTAC 
ACACCTGTCA ATGCATTTGA AGAGCGAGTG TCAGTGTTGT TCATCAGAAC AATACAGACT 
CGTTTACGGG ATCGAAAGGA GTCGCCTCAG CTTCTGATGG ACACCAAACT CATCTACCCC 
GTGACCTTCC CCTTTAACCC CTCCTCCCTT GCCCTGGAAA CCATTCAGAT CCCCAGCAGC 
CTCAATCTGG CCTTCCTCAC TCGTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292070.1
CDS	170..5719
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293795.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293795.2

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGG 
CTGTTGGAGT CTCAGTTGCA GGCTCAACGT CGCTCTCATG ACAACGAGGT GGAAGTTTTG 
CGTGGAGAGC TGCACAGTCT TAAGGAGGAG AACAACCGTC AGCAGCAGCT GTTGGCGCAG 
AACCTGCAAC TACCTCCAGA GGCCAGGATT GAAGCCAGTC TACAGCACGA GATCACACGC 
CTCACCAACG AGAACCTGGA ACTTCACTCT GATGATCCAA AGGAGTATAA AGCCTACAGA 
ATCAGCATGT TACGCAGGAT GGTTGATCTT ATGGAGCAGT TGGAGAAACA GGACAAGACC 
GTTCGTAAGC TGAAGAAACA GCTCAAAGTA TTTGCTAAGA AGATCAATGA TCTGGAAGGG 
GGGCAGATGG ACGTGTCTCC CGGACAAACG GCTGACGAGC CTGTTCATCC GGTGAACATT 
CCACGCAGAG AGAAAGATTT CCAGGGCATG CTGGAATACA AGAAAGAGGA TGAACTCAAA 
CTGGTCAAAA ATATCATACT TGAGCTGAAG CCTCGTGGTG TTGCAGTGAA TCTGATACCA 
GGTCTGCCCG CATACATCCT CTTCATGTGT CTGCGCCATG CTGACTACAT CAACGATGAC 
CAGAAAGTGC GATCTCTACT CACCTCCGTC ATCAACAGCA TCAAGAAGAT CCTCAAGAAA 
CGAGGTGATG ACTTTGAGAC TGTTTCCTTC TGGTTGGCCA ACACATGTCG TTTTCTGCAC 
TGCCTGAAAC AGTACAGTGG TGATGAGCAA TTCATGAAGC ATAATAGCCC AAAGCAGAAC 
GAACACTGTC TCTCTAACTT TGACCTGGCA GAGTACCGGC AGGTTCTGAG CGATCTGGCC 
ATTCAGATCT ACCAGCAGCT CATCAAGTGT ATGGAAAACA TCCTGCAGCC CATGATCGTC 
TCTGGCATGC TGGAACATGA AACCATCCAG GGTGTGTCAG GAGTGAAGCC CACAGGTCTC 
CGCAAGAGGA CGTCAAGTAT TGCTGATGAG GGCACGTACA CACTGGACTC CATCATCCGT 
CAGCTCAACA CCTTCCACTC CATCATGTGT CACCACGGCA CTGACCCCGA GCTCATCAAA 
CAGGTGGTCA AGCAGCAGTT CTACATCATC GGCGCTGTCA CCCTCAACAA CCTGCTGCTG 
CGCAAAGACA TGTGCTCCTG GAGCAAGGGC ATGCAGATCA GGTATAACGT GAGTCAGCTG 
GAGGAATGGC TGAGGGATAA GAACCTCATG ACATGCGGCG CTAAAGAAAC ACTGGAGCCG 
TTGATTCAGG CTGCGCAACT ACTGCAGGTG AAGAAGAAAA CTGATGAGGA CGCCGAGGCC 
ATCTGCTCTA TGTGTAACGC CCTCTCCACA GCACAGATTG TGAAAGTGCT GAACTTGTAC 
ACACCTGTCA ATGCATTTGA AGAGCGAGTG TCAGTGTTGT TCATCAGAAC AATACAGACT 
CGTTTACGGG ATCGAAAGGA GTCGCCTCAG CTTCTGATGG ACACCAAACT CATCTACCCC 
GTGACCTTCC CCTTTAACCC CTCCTCCCTT GCCCTGGAAA CCATTCAGAT CCCCAGCAGC 
CTCAATCTGG CCTTCCTCAC TCGTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293795.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292070.1 Danio rerio myosin VAa (myo5aa), transcript variant X2, mRNA.

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CloneID	ODa43731
Clone ID Related Accession (Same CDS sequence)	XM_009293795.2 , XM_009293795.3
Accession Version	XM_009293795.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293795.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGG 
CTGTTGGAGT CTCAGTTGCA GGCTCAACGT CGCTCTCATG ACAACGAGGT GGAAGTTTTG 
CGTGGAGAGC TGCACAGTCT TAAGGAGGAG AACAACCGTC AGCAGCAGCT GTTGGCGCAG 
AACCTGCAAC TACCTCCAGA GGCCAGGATT GAAGCCAGTC TACAGCACGA GATCACACGC 
CTCACCAACG AGAACCTGGA ACTTCACTCT GATGATCCAA AGGAGTATAA AGCCTACAGA 
ATCAGCATGT TACGCAGGAT GGTTGATCTT ATGGAGCAGT TGGAGAAACA GGACAAGACC 
GTTCGTAAGC TGAAGAAACA GCTCAAAGTA TTTGCTAAGA AGATCAATGA TCTGGAAGGG 
GGGCAGATGG ACGTGTCTCC CGGACAAACG GCTGACGAGC CTGTTCATCC GGTGAACATT 
CCACGCAGAG AGAAAGATTT CCAGGGCATG CTGGAATACA AGAAAGAGGA TGAACTCAAA 
CTGGTCAAAA ATATCATACT TGAGCTGAAG CCTCGTGGTG TTGCAGTGAA TCTGATACCA 
GGTCTGCCCG CATACATCCT CTTCATGTGT CTGCGCCATG CTGACTACAT CAACGATGAC 
CAGAAAGTGC GATCTCTACT CACCTCCGTC ATCAACAGCA TCAAGAAGAT CCTCAAGAAA 
CGAGGTGATG ACTTTGAGAC TGTTTCCTTC TGGTTGGCCA ACACATGTCG TTTTCTGCAC 
TGCCTGAAAC AGTACAGTGG TGATGAGCAA TTCATGAAGC ATAATAGCCC AAAGCAGAAC 
GAACACTGTC TCTCTAACTT TGACCTGGCA GAGTACCGGC AGGTTCTGAG CGATCTGGCC 
ATTCAGATCT ACCAGCAGCT CATCAAGTGT ATGGAAAACA TCCTGCAGCC CATGATCGTC 
TCTGGCATGC TGGAACATGA AACCATCCAG GGTGTGTCAG GAGTGAAGCC CACAGGTCTC 
CGCAAGAGGA CGTCAAGTAT TGCTGATGAG GGCACGTACA CACTGGACTC CATCATCCGT 
CAGCTCAACA CCTTCCACTC CATCATGTGT CACCACGGCA CTGACCCCGA GCTCATCAAA 
CAGGTGGTCA AGCAGCAGTT CTACATCATC GGCGCTGTCA CCCTCAACAA CCTGCTGCTG 
CGCAAAGACA TGTGCTCCTG GAGCAAGGGC ATGCAGATCA GGTATAACGT GAGTCAGCTG 
GAGGAATGGC TGAGGGATAA GAACCTCATG ACATGCGGCG CTAAAGAAAC ACTGGAGCCG 
TTGATTCAGG CTGCGCAACT ACTGCAGGTG AAGAAGAAAA CTGATGAGGA CGCCGAGGCC 
ATCTGCTCTA TGTGTAACGC CCTCTCCACA GCACAGATTG TGAAAGTGCT GAACTTGTAC 
ACACCTGTCA ATGCATTTGA AGAGCGAGTG TCAGTGTTGT TCATCAGAAC AATACAGACT 
CGTTTACGGG ATCGAAAGGA GTCGCCTCAG CTTCTGATGG ACACCAAACT CATCTACCCC 
GTGACCTTCC CCTTTAACCC CTCCTCCCTT GCCCTGGAAA CCATTCAGAT CCCCAGCAGC 
CTCAATCTGG CCTTCCTCAC TCGTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292070.1
CDS	165..5714
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293795.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293795.3

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGG ATGAGAAGAC GACTATGACT GAACCTCTGC CATACATTGA GGATGTGCAG 
AAGCTCACTG ATGCCAAGGA GATCTCACAG GCCTACATGG GCCTCAAAGA CACCAACAGG 
CTGTTGGAGT CTCAGTTGCA GGCTCAACGT CGCTCTCATG ACAACGAGGT GGAAGTTTTG 
CGTGGAGAGC TGCACAGTCT TAAGGAGGAG AACAACCGTC AGCAGCAGCT GTTGGCGCAG 
AACCTGCAAC TACCTCCAGA GGCCAGGATT GAAGCCAGTC TACAGCACGA GATCACACGC 
CTCACCAACG AGAACCTGGA ACTTCACTCT GATGATCCAA AGGAGTATAA AGCCTACAGA 
ATCAGCATGT TACGCAGGAT GGTTGATCTT ATGGAGCAGT TGGAGAAACA GGACAAGACC 
GTTCGTAAGC TGAAGAAACA GCTCAAAGTA TTTGCTAAGA AGATCAATGA TCTGGAAGGG 
GGGCAGATGG ACGTGTCTCC CGGACAAACG GCTGACGAGC CTGTTCATCC GGTGAACATT 
CCACGCAGAG AGAAAGATTT CCAGGGCATG CTGGAATACA AGAAAGAGGA TGAACTCAAA 
CTGGTCAAAA ATATCATACT TGAGCTGAAG CCTCGTGGTG TTGCAGTGAA TCTGATACCA 
GGTCTGCCCG CATACATCCT CTTCATGTGT CTGCGCCATG CTGACTACAT CAACGATGAC 
CAGAAAGTGC GATCTCTACT CACCTCCGTC ATCAACAGCA TCAAGAAGAT CCTCAAGAAA 
CGAGGTGATG ACTTTGAGAC TGTTTCCTTC TGGTTGGCCA ACACATGTCG TTTTCTGCAC 
TGCCTGAAAC AGTACAGTGG TGATGAGCAA TTCATGAAGC ATAATAGCCC AAAGCAGAAC 
GAACACTGTC TCTCTAACTT TGACCTGGCA GAGTACCGGC AGGTTCTGAG CGATCTGGCC 
ATTCAGATCT ACCAGCAGCT CATCAAGTGT ATGGAAAACA TCCTGCAGCC CATGATCGTC 
TCTGGCATGC TGGAACATGA AACCATCCAG GGTGTGTCAG GAGTGAAGCC CACAGGTCTC 
CGCAAGAGGA CGTCAAGTAT TGCTGATGAG GGCACGTACA CACTGGACTC CATCATCCGT 
CAGCTCAACA CCTTCCACTC CATCATGTGT CACCACGGCA CTGACCCCGA GCTCATCAAA 
CAGGTGGTCA AGCAGCAGTT CTACATCATC GGCGCTGTCA CCCTCAACAA CCTGCTGCTG 
CGCAAAGACA TGTGCTCCTG GAGCAAGGGC ATGCAGATCA GGTATAACGT GAGTCAGCTG 
GAGGAATGGC TGAGGGATAA GAACCTCATG ACATGCGGCG CTAAAGAAAC ACTGGAGCCG 
TTGATTCAGG CTGCGCAACT ACTGCAGGTG AAGAAGAAAA CTGATGAGGA CGCCGAGGCC 
ATCTGCTCTA TGTGTAACGC CCTCTCCACA GCACAGATTG TGAAAGTGCT GAACTTGTAC 
ACACCTGTCA ATGCATTTGA AGAGCGAGTG TCAGTGTTGT TCATCAGAAC AATACAGACT 
CGTTTACGGG ATCGAAAGGA GTCGCCTCAG CTTCTGATGG ACACCAAACT CATCTACCCC 
GTGACCTTCC CCTTTAACCC CTCCTCCCTT GCCCTGGAAA CCATTCAGAT CCCCAGCAGC 
CTCAATCTGG CCTTCCTCAC TCGTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293796.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292071.1 Danio rerio myosin VAa (myo5aa), transcript variant X3, mRNA.

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CloneID	ODa43732
Clone ID Related Accession (Same CDS sequence)	XM_009293796.2 , XM_009293796.3
Accession Version	XM_009293796.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	unconventional myosin-Va isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGT CTGAATTATA TACAAAGTAT GCACGGGTCT GGATCCCGGA TGACGAGGAG 
GTTTGGAGAT CAGCAGAGCT CACTAAAGAC TACAGACAAG GAGACGGAGT ACTGCAACTA 
CAGCTGGAGG ATGGAAAGGA CCTGGAGTTT AAATTGGACC CCAAGACGAA CAACTTGCCA 
CACCTGCGTA ACCCGGATAT CCTGGTGGGA GAAAATGACC TGACTGCTCT CAGCTACCTT 
CACGAACCTG CTGTGTTACA CAACCTGAAA GTACGCTTCA TCGACTCCAA GCTCATCTAC 
ACATACTGCG GTATTGTTCT AGTCGCTATA AACCCTTATG AGACTCTTCC CATCTATGGA 
GCGGACATCA TTAACGCATA CAGTGGGCAG AACATGGGCG ACATGGACCC TCACATCTTC 
GCAGTGGCTG AAGAAGCGTA TAAGCAGATG GCCAGGGATG AGAGGAATCA GTCTATTATT 
GTAAGTGGAG AATCTGGAGC GGGAAAAACG GTTTCGGCCA AATACGCCAT GCGTTACTTT 
GCCACAGTCA GTGGATCTGC CAGCGAAGCC AACGTGGAGG AGAAAGTTCT GGCATCCAAT 
CCCATAATGG AGTCTATTGG TAACGCCAAG ACCACCAGGA ATGACAACAG CAGTCGATTT 
GGGAAATACA TTGAGATTGG CTTTGATAAG AAGTATCACA TCATTGGTGC GAACATGAGG 
ACATATCTGC TGGAGAAATC ACGTGTGGTG TTTCAGGCGG ATGAAGAGAG GAATTATCAT 
ATCTTCTATC AGCTCTGTGC ATCGGCTCAC CTCCCGGAGT TTAAAGCCCT GAAACTCGGA 
AAGGCTAATG ATTTCCACTA CACAAAACAG GGCAGGAATC CAGTCATAGA TGGAGTAGAT 
GATGCCAAGG AGATGTCTAC CACCAGGAAC GCCTTCATAC TGCTAGGTAT AAATGAGTCC 
TATCAGATGG GCTTGTTTCA GATATTGGCT TCCATTTTGC ACCTTGGAAA TGTAGACGTG 
AAGGATAGAG ACTCGGACAG CAGTATTATT CCACCAAACA ACGGTCACCT GAGTGTGTTT 
TGTGAGTTGA TGGGAGTAAC ATATCAAGAC ATGTCTCATT GGCTCTGTCA TAAGAAGTTG 
AAGACTGCGA CTGAGACATA CATAAAGCCC ATACCCAAAC TACAGGCTAT AAACGCCCGC 
GACGCTCTCG CCAAACATAT CTACGCCAAA CTCTTCAACT GGATTGTAGA CCACGTGAAC 
AAAGCTCTGC ATTCAACGGT CAAGCAACAC TCTTTCATCG GAGTCTTGGA CATTTACGGG 
TTCGAGACTT TTGAAATCAA CAGTTTTGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTACAGCAAC AGTTCAACAT GCATGTGTTT AAGTTGGAGC AGGAGGAGTA TATGAAAGAG 
CAGATCCCGT GGACTCTCAT TGATTTCTAT GATAATCAGC CATGTATCAA CCTCATAGAG 
GCCAAGATGG GCATCCTGGA CCTGCTGGAT GAAGAGTGCA AGATGCCGAA GGGTTCGGAT 
GACTCCTGGG CTCAGAAACT CTATAACACT CATTTGAAGA CGTGTGCACT TTTTGAAAAG 
CCGCGGATGT CCAATAAAGC TTTCATTATT CAGCACTTTG CAGATAAGGT GGAGTATCAA 
TGTGATGGTT TTCTGGAGAA GAATAAAGAC ACGGTGAATG AGGAGCAGAT AAATGTTTTG 
AAGGCCAGTA AGAAGTTTGA CCTGCTAGTG GAGCTGTTCC ATGATGAGGA GAAAGCCACA 
AGTCCCACCG GTGCTGCCCC TGGCCCTGGA GGACGCACTC GCCTCAGTGT CAAACCAGAC 
AAGGGCAAAT CCAGCCAAGC CAGCAAAGAA CACAAGAAGA CAGTCGGACT GCAGTTCCGG 
AACTCTCTGC AGCTGCTGAT GGAGACACTG AATGCCACTA CACCTCATTA TGTTCGATGC 
ATTAAACCCA ACGATTACAA ACATGCCTTC ACGTTTGACC CCAAGAGGGC AGTGCAGCAA 
CTGCGTGCTT GTGGAGTTTT GGAAACAATC CGCATCTCTG CAGCTGGTTT CCCATCGAGG 
TGGACGTATC AAGAGTTCTT TAGCAGGTAT CGTGTACTGA TGAAACAAAA AGATGTTCTG 
ACGGACAAGA AGATGACCTG TAAGAATGTT CTGGAGAAAC TGGTGCAGGA TCCAGATAAG 
TACCAGTTTG GAAAGACCAA GATTTTTTTC CGCGCAGGTC AGGTTGCATA CCTGGAGAAA 
CTGCGTGCTG ATAAGCTGCG CGCTGCCTGT ATCCGCATCC AGAAGACCAT CCGCTGCTGG 
CTGGCACGCA AGAAGTACCT GCGTATGAAG CATGCTGCCA CGACTATCCA GAGATTCGTC 
CGTGGATACC AGGCCCGCTG TCTTGCCAAA TTCCTGCGTC GCACACGTGC TGCGATCATT 
ATTCAGAAGT ACCAGCGCAT GTACATCCAG AAAACGTGTT ACAAACGCAA GCAGGCTGCC 
GCGCTGGCCA TGCAGTGCAT CCTCAGAGCG TATATGGCCA GACAGCTTTA CAAAGCGCTG 
CTGCGTGAGC ACAAGGCTGT CATTATTCAG AAGATGGTGC GTGGCTGGCT GGCTCGGCAG 
TGGTTCAAGC GCTCGCTCAA AGCCATCGTT TACCTGCAGT GCTGCATTCG GCGAATGCGA 
GCCAAACGAG AACTCAAGAA GCTCAAGATC GAAGCACGCT CGGTCGAACA CTTCAAGAAG 
CTCAACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAGGATAGA CGATCAGAAC 
AAGGAGAACC GTTCCATGAG CGAACGTTTG AACACTCTAG AGACCAGCCA TGCTGTTGAG 
TCAGAGCGTA TGCGCGCCGA AGTGACGCGT CTGCGGGGAG CAGAGGAGGA CGCCAAAAAC 
AACGCCAACA AAGTCACATC TCTTCTGGAG GAGCTGGAAC GACTGCGCAA AGACCTGCAG 
AACACACAGA AGGAGAAGAA AGCCATTGAG GACTGGGCTC AGACCTACCA GGACGAGATG 
GAAAAGATGA TCTCAGAGCT AAAAGAGCAA AACCAGCTGC TGAAGACAGA GAAAAACAAC 
CTAAACCAAC TGATCCAGGA ACAGAGCCAG CAGTGGACAG ACAAAATGCA GAGAGCTCTG 
AAAGAGGAAA CCCAGCAGTT AGAAAACGAT TTAAACGAGG AGCGATCTCG CTACCAAAAT 
CTCCTCACCG AGCACCTCCG ACTAGAGGAG AAATACGACG ATTTGAAAGA AGAGATCACA 
CTCGCTGTGA ATGTTCCAAA ACCTGGTCAC CGGCGCACAG ACTCCACACA CAGCAGCAAC 
GAATCAGAAT GTACCTACAA CTCAGAGTTC GCAGAGTCAG AGGAGGGATC CCGCGTCGGG 
GAGGATGTTT CCAAGTCAAT CCTGGACATG TCTCTGTTCC TGAAGCTGCA GAAGAGAGTT 
TCCGAACTCG AGCAAGAAAA GCAGTCGCTG CAGAACGAAC TGGATCGCAG AGAAGAGCAG 
TTTCAGCGGG CTAGAGCTAG GGATGATGAA GAACATAAGA AAGCTCGTGG TGCAGAGCTG 
GAGTATGAAT CTCTCAAGCG TCAGGAGCTG GAGTCTGAGA ACAAGAAGCT GAAGCATGAT 
CTGAATCAGA TGCGTCAGTC TCTCAGAGGC AGCGTTGAGG CCGGTTCTGG TGCTCCTGGT 
TCTCCTGCGT ACACCGTTCT TCTGGATCAG CTCAATGCCT CCAACGAGGA ACTGGAAGTG 
CGCAAAGAGG AGGTGCTCAT CCTGCGCTCA CAGCTCGTCA GTCAGAAAGA AGCCATGCAG 
CACAAGGATG AGAAGACGAC TATGACTGAA CCTCTGCCAT ACATTGAGGA TGTGCAGAAG 
CTCACTGATG CCAAGGAGAT CTCACAGGCC TACATGGGCC TCAAAGACAC CAACAGGCTG 
TTGGAGTCTC AGTTGCAGGC TCAACGTCGC TCTCATGACA ACGAGGTGGA AGTTTTGCGT 
GGAGAGCTGC ACAGTCTTAA GGAGGAGAAC AACCGTCAGC AGCAGCTGTT GGCGCAGAAC 
CTGCAACTAC CTCCAGAGGC CAGGATTGAA GCCAGTCTAC AGCACGAGAT CACACGCCTC 
ACCAACGAGA ACCTGGAACT TCACTCTGAT GATCCAAAGG AGTATAAAGC CTACAGAATC 
AGCATGTTAC GCAGGATGGT TGATCTTATG GAGCAGTTGG AGAAACAGGA CAAGACCGTT 
CGTAAGCTGA AGAAACAGCT CAAAGTATTT GCTAAGAAGA TCAATGATCT GGAAGGGGGG 
CAGATGGACG TGTCTCCCGG ACAAACGGCT GACGAGCCTG TTCATCCGGT GAACATTCCA 
CGCAGAGAGA AAGATTTCCA GGGCATGCTG GAATACAAGA AAGAGGATGA ACTCAAACTG 
GTCAAAAATA TCATACTTGA GCTGAAGCCT CGTGGTGTTG CAGTGAATCT GATACCAGGT 
CTGCCCGCAT ACATCCTCTT CATGTGTCTG CGCCATGCTG ACTACATCAA CGATGACCAG 
AAAGTGCGAT CTCTACTCAC CTCCGTCATC AACAGCATCA AGAAGATCCT CAAGAAACGA 
GGTGATGACT TTGAGACTGT TTCCTTCTGG TTGGCCAACA CATGTCGTTT TCTGCACTGC 
CTGAAACAGT ACAGTGGTGA TGAGCAATTC ATGAAGCATA ATAGCCCAAA GCAGAACGAA 
CACTGTCTCT CTAACTTTGA CCTGGCAGAG TACCGGCAGG TTCTGAGCGA TCTGGCCATT 
CAGATCTACC AGCAGCTCAT CAAGTGTATG GAAAACATCC TGCAGCCCAT GATCGTCTCT 
GGCATGCTGG AACATGAAAC CATCCAGGGT GTGTCAGGAG TGAAGCCCAC AGGTCTCCGC 
AAGAGGACGT CAAGTATTGC TGATGAGGGC ACGTACACAC TGGACTCCAT CATCCGTCAG 
CTCAACACCT TCCACTCCAT CATGTGTCAC CACGGCACTG ACCCCGAGCT CATCAAACAG 
GTGGTCAAGC AGCAGTTCTA CATCATCGGC GCTGTCACCC TCAACAACCT GCTGCTGCGC 
AAAGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ATAACGTGAG TCAGCTGGAG 
GAATGGCTGA GGGATAAGAA CCTCATGACA TGCGGCGCTA AAGAAACACT GGAGCCGTTG 
ATTCAGGCTG CGCAACTACT GCAGGTGAAG AAGAAAACTG ATGAGGACGC CGAGGCCATC 
TGCTCTATGT GTAACGCCCT CTCCACAGCA CAGATTGTGA AAGTGCTGAA CTTGTACACA 
CCTGTCAATG CATTTGAAGA GCGAGTGTCA GTGTTGTTCA TCAGAACAAT ACAGACTCGT 
TTACGGGATC GAAAGGAGTC GCCTCAGCTT CTGATGGACA CCAAACTCAT CTACCCCGTG 
ACCTTCCCCT TTAACCCCTC CTCCCTTGCC CTGGAAACCA TTCAGATCCC CAGCAGCCTC 
AATCTGGCCT TCCTCACTCG TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292071.1
CDS	382..5928
Translation	MAASELYTKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293796.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293796.2

ORF Insert Sequence:

ATGGCTGCGT CTGAATTATA TACAAAGTAT GCACGGGTCT GGATCCCGGA TGACGAGGAG 
GTTTGGAGAT CAGCAGAGCT CACTAAAGAC TACAGACAAG GAGACGGAGT ACTGCAACTA 
CAGCTGGAGG ATGGAAAGGA CCTGGAGTTT AAATTGGACC CCAAGACGAA CAACTTGCCA 
CACCTGCGTA ACCCGGATAT CCTGGTGGGA GAAAATGACC TGACTGCTCT CAGCTACCTT 
CACGAACCTG CTGTGTTACA CAACCTGAAA GTACGCTTCA TCGACTCCAA GCTCATCTAC 
ACATACTGCG GTATTGTTCT AGTCGCTATA AACCCTTATG AGACTCTTCC CATCTATGGA 
GCGGACATCA TTAACGCATA CAGTGGGCAG AACATGGGCG ACATGGACCC TCACATCTTC 
GCAGTGGCTG AAGAAGCGTA TAAGCAGATG GCCAGGGATG AGAGGAATCA GTCTATTATT 
GTAAGTGGAG AATCTGGAGC GGGAAAAACG GTTTCGGCCA AATACGCCAT GCGTTACTTT 
GCCACAGTCA GTGGATCTGC CAGCGAAGCC AACGTGGAGG AGAAAGTTCT GGCATCCAAT 
CCCATAATGG AGTCTATTGG TAACGCCAAG ACCACCAGGA ATGACAACAG CAGTCGATTT 
GGGAAATACA TTGAGATTGG CTTTGATAAG AAGTATCACA TCATTGGTGC GAACATGAGG 
ACATATCTGC TGGAGAAATC ACGTGTGGTG TTTCAGGCGG ATGAAGAGAG GAATTATCAT 
ATCTTCTATC AGCTCTGTGC ATCGGCTCAC CTCCCGGAGT TTAAAGCCCT GAAACTCGGA 
AAGGCTAATG ATTTCCACTA CACAAAACAG GGCAGGAATC CAGTCATAGA TGGAGTAGAT 
GATGCCAAGG AGATGTCTAC CACCAGGAAC GCCTTCATAC TGCTAGGTAT AAATGAGTCC 
TATCAGATGG GCTTGTTTCA GATATTGGCT TCCATTTTGC ACCTTGGAAA TGTAGACGTG 
AAGGATAGAG ACTCGGACAG CAGTATTATT CCACCAAACA ACGGTCACCT GAGTGTGTTT 
TGTGAGTTGA TGGGAGTAAC ATATCAAGAC ATGTCTCATT GGCTCTGTCA TAAGAAGTTG 
AAGACTGCGA CTGAGACATA CATAAAGCCC ATACCCAAAC TACAGGCTAT AAACGCCCGC 
GACGCTCTCG CCAAACATAT CTACGCCAAA CTCTTCAACT GGATTGTAGA CCACGTGAAC 
AAAGCTCTGC ATTCAACGGT CAAGCAACAC TCTTTCATCG GAGTCTTGGA CATTTACGGG 
TTCGAGACTT TTGAAATCAA CAGTTTTGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTACAGCAAC AGTTCAACAT GCATGTGTTT AAGTTGGAGC AGGAGGAGTA TATGAAAGAG 
CAGATCCCGT GGACTCTCAT TGATTTCTAT GATAATCAGC CATGTATCAA CCTCATAGAG 
GCCAAGATGG GCATCCTGGA CCTGCTGGAT GAAGAGTGCA AGATGCCGAA GGGTTCGGAT 
GACTCCTGGG CTCAGAAACT CTATAACACT CATTTGAAGA CGTGTGCACT TTTTGAAAAG 
CCGCGGATGT CCAATAAAGC TTTCATTATT CAGCACTTTG CAGATAAGGT GGAGTATCAA 
TGTGATGGTT TTCTGGAGAA GAATAAAGAC ACGGTGAATG AGGAGCAGAT AAATGTTTTG 
AAGGCCAGTA AGAAGTTTGA CCTGCTAGTG GAGCTGTTCC ATGATGAGGA GAAAGCCACA 
AGTCCCACCG GTGCTGCCCC TGGCCCTGGA GGACGCACTC GCCTCAGTGT CAAACCAGAC 
AAGGGCAAAT CCAGCCAAGC CAGCAAAGAA CACAAGAAGA CAGTCGGACT GCAGTTCCGG 
AACTCTCTGC AGCTGCTGAT GGAGACACTG AATGCCACTA CACCTCATTA TGTTCGATGC 
ATTAAACCCA ACGATTACAA ACATGCCTTC ACGTTTGACC CCAAGAGGGC AGTGCAGCAA 
CTGCGTGCTT GTGGAGTTTT GGAAACAATC CGCATCTCTG CAGCTGGTTT CCCATCGAGG 
TGGACGTATC AAGAGTTCTT TAGCAGGTAT CGTGTACTGA TGAAACAAAA AGATGTTCTG 
ACGGACAAGA AGATGACCTG TAAGAATGTT CTGGAGAAAC TGGTGCAGGA TCCAGATAAG 
TACCAGTTTG GAAAGACCAA GATTTTTTTC CGCGCAGGTC AGGTTGCATA CCTGGAGAAA 
CTGCGTGCTG ATAAGCTGCG CGCTGCCTGT ATCCGCATCC AGAAGACCAT CCGCTGCTGG 
CTGGCACGCA AGAAGTACCT GCGTATGAAG CATGCTGCCA CGACTATCCA GAGATTCGTC 
CGTGGATACC AGGCCCGCTG TCTTGCCAAA TTCCTGCGTC GCACACGTGC TGCGATCATT 
ATTCAGAAGT ACCAGCGCAT GTACATCCAG AAAACGTGTT ACAAACGCAA GCAGGCTGCC 
GCGCTGGCCA TGCAGTGCAT CCTCAGAGCG TATATGGCCA GACAGCTTTA CAAAGCGCTG 
CTGCGTGAGC ACAAGGCTGT CATTATTCAG AAGATGGTGC GTGGCTGGCT GGCTCGGCAG 
TGGTTCAAGC GCTCGCTCAA AGCCATCGTT TACCTGCAGT GCTGCATTCG GCGAATGCGA 
GCCAAACGAG AACTCAAGAA GCTCAAGATC GAAGCACGCT CGGTCGAACA CTTCAAGAAG 
CTCAACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAGGATAGA CGATCAGAAC 
AAGGAGAACC GTTCCATGAG CGAACGTTTG AACACTCTAG AGACCAGCCA TGCTGTTGAG 
TCAGAGCGTA TGCGCGCCGA AGTGACGCGT CTGCGGGGAG CAGAGGAGGA CGCCAAAAAC 
AACGCCAACA AAGTCACATC TCTTCTGGAG GAGCTGGAAC GACTGCGCAA AGACCTGCAG 
AACACACAGA AGGAGAAGAA AGCCATTGAG GACTGGGCTC AGACCTACCA GGACGAGATG 
GAAAAGATGA TCTCAGAGCT AAAAGAGCAA AACCAGCTGC TGAAGACAGA GAAAAACAAC 
CTAAACCAAC TGATCCAGGA ACAGAGCCAG CAGTGGACAG ACAAAATGCA GAGAGCTCTG 
AAAGAGGAAA CCCAGCAGTT AGAAAACGAT TTAAACGAGG AGCGATCTCG CTACCAAAAT 
CTCCTCACCG AGCACCTCCG ACTAGAGGAG AAATACGACG ATTTGAAAGA AGAGATCACA 
CTCGCTGTGA ATGTTCCAAA ACCTGGTCAC CGGCGCACAG ACTCCACACA CAGCAGCAAC 
GAATCAGAAT GTACCTACAA CTCAGAGTTC GCAGAGTCAG AGGAGGGATC CCGCGTCGGG 
GAGGATGTTT CCAAGTCAAT CCTGGACATG TCTCTGTTCC TGAAGCTGCA GAAGAGAGTT 
TCCGAACTCG AGCAAGAAAA GCAGTCGCTG CAGAACGAAC TGGATCGCAG AGAAGAGCAG 
TTTCAGCGGG CTAGAGCTAG GGATGATGAA GAACATAAGA AAGCTCGTGG TGCAGAGCTG 
GAGTATGAAT CTCTCAAGCG TCAGGAGCTG GAGTCTGAGA ACAAGAAGCT GAAGCATGAT 
CTGAATCAGA TGCGTCAGTC TCTCAGAGGC AGCGTTGAGG CCGGTTCTGG TGCTCCTGGT 
TCTCCTGCGT ACACCGTTCT TCTGGATCAG CTCAATGCCT CCAACGAGGA ACTGGAAGTG 
CGCAAAGAGG AGGTGCTCAT CCTGCGCTCA CAGCTCGTCA GTCAGAAAGA AGCCATGCAG 
CACAAGGATG AGAAGACGAC TATGACTGAA CCTCTGCCAT ACATTGAGGA TGTGCAGAAG 
CTCACTGATG CCAAGGAGAT CTCACAGGCC TACATGGGCC TCAAAGACAC CAACAGGCTG 
TTGGAGTCTC AGTTGCAGGC TCAACGTCGC TCTCATGACA ACGAGGTGGA AGTTTTGCGT 
GGAGAGCTGC ACAGTCTTAA GGAGGAGAAC AACCGTCAGC AGCAGCTGTT GGCGCAGAAC 
CTGCAACTAC CTCCAGAGGC CAGGATTGAA GCCAGTCTAC AGCACGAGAT CACACGCCTC 
ACCAACGAGA ACCTGGAACT TCACTCTGAT GATCCAAAGG AGTATAAAGC CTACAGAATC 
AGCATGTTAC GCAGGATGGT TGATCTTATG GAGCAGTTGG AGAAACAGGA CAAGACCGTT 
CGTAAGCTGA AGAAACAGCT CAAAGTATTT GCTAAGAAGA TCAATGATCT GGAAGGGGGG 
CAGATGGACG TGTCTCCCGG ACAAACGGCT GACGAGCCTG TTCATCCGGT GAACATTCCA 
CGCAGAGAGA AAGATTTCCA GGGCATGCTG GAATACAAGA AAGAGGATGA ACTCAAACTG 
GTCAAAAATA TCATACTTGA GCTGAAGCCT CGTGGTGTTG CAGTGAATCT GATACCAGGT 
CTGCCCGCAT ACATCCTCTT CATGTGTCTG CGCCATGCTG ACTACATCAA CGATGACCAG 
AAAGTGCGAT CTCTACTCAC CTCCGTCATC AACAGCATCA AGAAGATCCT CAAGAAACGA 
GGTGATGACT TTGAGACTGT TTCCTTCTGG TTGGCCAACA CATGTCGTTT TCTGCACTGC 
CTGAAACAGT ACAGTGGTGA TGAGCAATTC ATGAAGCATA ATAGCCCAAA GCAGAACGAA 
CACTGTCTCT CTAACTTTGA CCTGGCAGAG TACCGGCAGG TTCTGAGCGA TCTGGCCATT 
CAGATCTACC AGCAGCTCAT CAAGTGTATG GAAAACATCC TGCAGCCCAT GATCGTCTCT 
GGCATGCTGG AACATGAAAC CATCCAGGGT GTGTCAGGAG TGAAGCCCAC AGGTCTCCGC 
AAGAGGACGT CAAGTATTGC TGATGAGGGC ACGTACACAC TGGACTCCAT CATCCGTCAG 
CTCAACACCT TCCACTCCAT CATGTGTCAC CACGGCACTG ACCCCGAGCT CATCAAACAG 
GTGGTCAAGC AGCAGTTCTA CATCATCGGC GCTGTCACCC TCAACAACCT GCTGCTGCGC 
AAAGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ATAACGTGAG TCAGCTGGAG 
GAATGGCTGA GGGATAAGAA CCTCATGACA TGCGGCGCTA AAGAAACACT GGAGCCGTTG 
ATTCAGGCTG CGCAACTACT GCAGGTGAAG AAGAAAACTG ATGAGGACGC CGAGGCCATC 
TGCTCTATGT GTAACGCCCT CTCCACAGCA CAGATTGTGA AAGTGCTGAA CTTGTACACA 
CCTGTCAATG CATTTGAAGA GCGAGTGTCA GTGTTGTTCA TCAGAACAAT ACAGACTCGT 
TTACGGGATC GAAAGGAGTC GCCTCAGCTT CTGATGGACA CCAAACTCAT CTACCCCGTG 
ACCTTCCCCT TTAACCCCTC CTCCCTTGCC CTGGAAACCA TTCAGATCCC CAGCAGCCTC 
AATCTGGCCT TCCTCACTCG TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293796.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292071.1 Danio rerio myosin VAa (myo5aa), transcript variant X3, mRNA.

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CloneID	ODa43732
Clone ID Related Accession (Same CDS sequence)	XM_009293796.2 , XM_009293796.3
Accession Version	XM_009293796.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293796.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGT CTGAATTATA TACAAAGTAT GCACGGGTCT GGATCCCGGA TGACGAGGAG 
GTTTGGAGAT CAGCAGAGCT CACTAAAGAC TACAGACAAG GAGACGGAGT ACTGCAACTA 
CAGCTGGAGG ATGGAAAGGA CCTGGAGTTT AAATTGGACC CCAAGACGAA CAACTTGCCA 
CACCTGCGTA ACCCGGATAT CCTGGTGGGA GAAAATGACC TGACTGCTCT CAGCTACCTT 
CACGAACCTG CTGTGTTACA CAACCTGAAA GTACGCTTCA TCGACTCCAA GCTCATCTAC 
ACATACTGCG GTATTGTTCT AGTCGCTATA AACCCTTATG AGACTCTTCC CATCTATGGA 
GCGGACATCA TTAACGCATA CAGTGGGCAG AACATGGGCG ACATGGACCC TCACATCTTC 
GCAGTGGCTG AAGAAGCGTA TAAGCAGATG GCCAGGGATG AGAGGAATCA GTCTATTATT 
GTAAGTGGAG AATCTGGAGC GGGAAAAACG GTTTCGGCCA AATACGCCAT GCGTTACTTT 
GCCACAGTCA GTGGATCTGC CAGCGAAGCC AACGTGGAGG AGAAAGTTCT GGCATCCAAT 
CCCATAATGG AGTCTATTGG TAACGCCAAG ACCACCAGGA ATGACAACAG CAGTCGATTT 
GGGAAATACA TTGAGATTGG CTTTGATAAG AAGTATCACA TCATTGGTGC GAACATGAGG 
ACATATCTGC TGGAGAAATC ACGTGTGGTG TTTCAGGCGG ATGAAGAGAG GAATTATCAT 
ATCTTCTATC AGCTCTGTGC ATCGGCTCAC CTCCCGGAGT TTAAAGCCCT GAAACTCGGA 
AAGGCTAATG ATTTCCACTA CACAAAACAG GGCAGGAATC CAGTCATAGA TGGAGTAGAT 
GATGCCAAGG AGATGTCTAC CACCAGGAAC GCCTTCATAC TGCTAGGTAT AAATGAGTCC 
TATCAGATGG GCTTGTTTCA GATATTGGCT TCCATTTTGC ACCTTGGAAA TGTAGACGTG 
AAGGATAGAG ACTCGGACAG CAGTATTATT CCACCAAACA ACGGTCACCT GAGTGTGTTT 
TGTGAGTTGA TGGGAGTAAC ATATCAAGAC ATGTCTCATT GGCTCTGTCA TAAGAAGTTG 
AAGACTGCGA CTGAGACATA CATAAAGCCC ATACCCAAAC TACAGGCTAT AAACGCCCGC 
GACGCTCTCG CCAAACATAT CTACGCCAAA CTCTTCAACT GGATTGTAGA CCACGTGAAC 
AAAGCTCTGC ATTCAACGGT CAAGCAACAC TCTTTCATCG GAGTCTTGGA CATTTACGGG 
TTCGAGACTT TTGAAATCAA CAGTTTTGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTACAGCAAC AGTTCAACAT GCATGTGTTT AAGTTGGAGC AGGAGGAGTA TATGAAAGAG 
CAGATCCCGT GGACTCTCAT TGATTTCTAT GATAATCAGC CATGTATCAA CCTCATAGAG 
GCCAAGATGG GCATCCTGGA CCTGCTGGAT GAAGAGTGCA AGATGCCGAA GGGTTCGGAT 
GACTCCTGGG CTCAGAAACT CTATAACACT CATTTGAAGA CGTGTGCACT TTTTGAAAAG 
CCGCGGATGT CCAATAAAGC TTTCATTATT CAGCACTTTG CAGATAAGGT GGAGTATCAA 
TGTGATGGTT TTCTGGAGAA GAATAAAGAC ACGGTGAATG AGGAGCAGAT AAATGTTTTG 
AAGGCCAGTA AGAAGTTTGA CCTGCTAGTG GAGCTGTTCC ATGATGAGGA GAAAGCCACA 
AGTCCCACCG GTGCTGCCCC TGGCCCTGGA GGACGCACTC GCCTCAGTGT CAAACCAGAC 
AAGGGCAAAT CCAGCCAAGC CAGCAAAGAA CACAAGAAGA CAGTCGGACT GCAGTTCCGG 
AACTCTCTGC AGCTGCTGAT GGAGACACTG AATGCCACTA CACCTCATTA TGTTCGATGC 
ATTAAACCCA ACGATTACAA ACATGCCTTC ACGTTTGACC CCAAGAGGGC AGTGCAGCAA 
CTGCGTGCTT GTGGAGTTTT GGAAACAATC CGCATCTCTG CAGCTGGTTT CCCATCGAGG 
TGGACGTATC AAGAGTTCTT TAGCAGGTAT CGTGTACTGA TGAAACAAAA AGATGTTCTG 
ACGGACAAGA AGATGACCTG TAAGAATGTT CTGGAGAAAC TGGTGCAGGA TCCAGATAAG 
TACCAGTTTG GAAAGACCAA GATTTTTTTC CGCGCAGGTC AGGTTGCATA CCTGGAGAAA 
CTGCGTGCTG ATAAGCTGCG CGCTGCCTGT ATCCGCATCC AGAAGACCAT CCGCTGCTGG 
CTGGCACGCA AGAAGTACCT GCGTATGAAG CATGCTGCCA CGACTATCCA GAGATTCGTC 
CGTGGATACC AGGCCCGCTG TCTTGCCAAA TTCCTGCGTC GCACACGTGC TGCGATCATT 
ATTCAGAAGT ACCAGCGCAT GTACATCCAG AAAACGTGTT ACAAACGCAA GCAGGCTGCC 
GCGCTGGCCA TGCAGTGCAT CCTCAGAGCG TATATGGCCA GACAGCTTTA CAAAGCGCTG 
CTGCGTGAGC ACAAGGCTGT CATTATTCAG AAGATGGTGC GTGGCTGGCT GGCTCGGCAG 
TGGTTCAAGC GCTCGCTCAA AGCCATCGTT TACCTGCAGT GCTGCATTCG GCGAATGCGA 
GCCAAACGAG AACTCAAGAA GCTCAAGATC GAAGCACGCT CGGTCGAACA CTTCAAGAAG 
CTCAACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAGGATAGA CGATCAGAAC 
AAGGAGAACC GTTCCATGAG CGAACGTTTG AACACTCTAG AGACCAGCCA TGCTGTTGAG 
TCAGAGCGTA TGCGCGCCGA AGTGACGCGT CTGCGGGGAG CAGAGGAGGA CGCCAAAAAC 
AACGCCAACA AAGTCACATC TCTTCTGGAG GAGCTGGAAC GACTGCGCAA AGACCTGCAG 
AACACACAGA AGGAGAAGAA AGCCATTGAG GACTGGGCTC AGACCTACCA GGACGAGATG 
GAAAAGATGA TCTCAGAGCT AAAAGAGCAA AACCAGCTGC TGAAGACAGA GAAAAACAAC 
CTAAACCAAC TGATCCAGGA ACAGAGCCAG CAGTGGACAG ACAAAATGCA GAGAGCTCTG 
AAAGAGGAAA CCCAGCAGTT AGAAAACGAT TTAAACGAGG AGCGATCTCG CTACCAAAAT 
CTCCTCACCG AGCACCTCCG ACTAGAGGAG AAATACGACG ATTTGAAAGA AGAGATCACA 
CTCGCTGTGA ATGTTCCAAA ACCTGGTCAC CGGCGCACAG ACTCCACACA CAGCAGCAAC 
GAATCAGAAT GTACCTACAA CTCAGAGTTC GCAGAGTCAG AGGAGGGATC CCGCGTCGGG 
GAGGATGTTT CCAAGTCAAT CCTGGACATG TCTCTGTTCC TGAAGCTGCA GAAGAGAGTT 
TCCGAACTCG AGCAAGAAAA GCAGTCGCTG CAGAACGAAC TGGATCGCAG AGAAGAGCAG 
TTTCAGCGGG CTAGAGCTAG GGATGATGAA GAACATAAGA AAGCTCGTGG TGCAGAGCTG 
GAGTATGAAT CTCTCAAGCG TCAGGAGCTG GAGTCTGAGA ACAAGAAGCT GAAGCATGAT 
CTGAATCAGA TGCGTCAGTC TCTCAGAGGC AGCGTTGAGG CCGGTTCTGG TGCTCCTGGT 
TCTCCTGCGT ACACCGTTCT TCTGGATCAG CTCAATGCCT CCAACGAGGA ACTGGAAGTG 
CGCAAAGAGG AGGTGCTCAT CCTGCGCTCA CAGCTCGTCA GTCAGAAAGA AGCCATGCAG 
CACAAGGATG AGAAGACGAC TATGACTGAA CCTCTGCCAT ACATTGAGGA TGTGCAGAAG 
CTCACTGATG CCAAGGAGAT CTCACAGGCC TACATGGGCC TCAAAGACAC CAACAGGCTG 
TTGGAGTCTC AGTTGCAGGC TCAACGTCGC TCTCATGACA ACGAGGTGGA AGTTTTGCGT 
GGAGAGCTGC ACAGTCTTAA GGAGGAGAAC AACCGTCAGC AGCAGCTGTT GGCGCAGAAC 
CTGCAACTAC CTCCAGAGGC CAGGATTGAA GCCAGTCTAC AGCACGAGAT CACACGCCTC 
ACCAACGAGA ACCTGGAACT TCACTCTGAT GATCCAAAGG AGTATAAAGC CTACAGAATC 
AGCATGTTAC GCAGGATGGT TGATCTTATG GAGCAGTTGG AGAAACAGGA CAAGACCGTT 
CGTAAGCTGA AGAAACAGCT CAAAGTATTT GCTAAGAAGA TCAATGATCT GGAAGGGGGG 
CAGATGGACG TGTCTCCCGG ACAAACGGCT GACGAGCCTG TTCATCCGGT GAACATTCCA 
CGCAGAGAGA AAGATTTCCA GGGCATGCTG GAATACAAGA AAGAGGATGA ACTCAAACTG 
GTCAAAAATA TCATACTTGA GCTGAAGCCT CGTGGTGTTG CAGTGAATCT GATACCAGGT 
CTGCCCGCAT ACATCCTCTT CATGTGTCTG CGCCATGCTG ACTACATCAA CGATGACCAG 
AAAGTGCGAT CTCTACTCAC CTCCGTCATC AACAGCATCA AGAAGATCCT CAAGAAACGA 
GGTGATGACT TTGAGACTGT TTCCTTCTGG TTGGCCAACA CATGTCGTTT TCTGCACTGC 
CTGAAACAGT ACAGTGGTGA TGAGCAATTC ATGAAGCATA ATAGCCCAAA GCAGAACGAA 
CACTGTCTCT CTAACTTTGA CCTGGCAGAG TACCGGCAGG TTCTGAGCGA TCTGGCCATT 
CAGATCTACC AGCAGCTCAT CAAGTGTATG GAAAACATCC TGCAGCCCAT GATCGTCTCT 
GGCATGCTGG AACATGAAAC CATCCAGGGT GTGTCAGGAG TGAAGCCCAC AGGTCTCCGC 
AAGAGGACGT CAAGTATTGC TGATGAGGGC ACGTACACAC TGGACTCCAT CATCCGTCAG 
CTCAACACCT TCCACTCCAT CATGTGTCAC CACGGCACTG ACCCCGAGCT CATCAAACAG 
GTGGTCAAGC AGCAGTTCTA CATCATCGGC GCTGTCACCC TCAACAACCT GCTGCTGCGC 
AAAGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ATAACGTGAG TCAGCTGGAG 
GAATGGCTGA GGGATAAGAA CCTCATGACA TGCGGCGCTA AAGAAACACT GGAGCCGTTG 
ATTCAGGCTG CGCAACTACT GCAGGTGAAG AAGAAAACTG ATGAGGACGC CGAGGCCATC 
TGCTCTATGT GTAACGCCCT CTCCACAGCA CAGATTGTGA AAGTGCTGAA CTTGTACACA 
CCTGTCAATG CATTTGAAGA GCGAGTGTCA GTGTTGTTCA TCAGAACAAT ACAGACTCGT 
TTACGGGATC GAAAGGAGTC GCCTCAGCTT CTGATGGACA CCAAACTCAT CTACCCCGTG 
ACCTTCCCCT TTAACCCCTC CTCCCTTGCC CTGGAAACCA TTCAGATCCC CAGCAGCCTC 
AATCTGGCCT TCCTCACTCG TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292071.1
CDS	252..5798
Translation	MAASELYTKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKDEKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293796.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293796.3

ORF Insert Sequence:

ATGGCTGCGT CTGAATTATA TACAAAGTAT GCACGGGTCT GGATCCCGGA TGACGAGGAG 
GTTTGGAGAT CAGCAGAGCT CACTAAAGAC TACAGACAAG GAGACGGAGT ACTGCAACTA 
CAGCTGGAGG ATGGAAAGGA CCTGGAGTTT AAATTGGACC CCAAGACGAA CAACTTGCCA 
CACCTGCGTA ACCCGGATAT CCTGGTGGGA GAAAATGACC TGACTGCTCT CAGCTACCTT 
CACGAACCTG CTGTGTTACA CAACCTGAAA GTACGCTTCA TCGACTCCAA GCTCATCTAC 
ACATACTGCG GTATTGTTCT AGTCGCTATA AACCCTTATG AGACTCTTCC CATCTATGGA 
GCGGACATCA TTAACGCATA CAGTGGGCAG AACATGGGCG ACATGGACCC TCACATCTTC 
GCAGTGGCTG AAGAAGCGTA TAAGCAGATG GCCAGGGATG AGAGGAATCA GTCTATTATT 
GTAAGTGGAG AATCTGGAGC GGGAAAAACG GTTTCGGCCA AATACGCCAT GCGTTACTTT 
GCCACAGTCA GTGGATCTGC CAGCGAAGCC AACGTGGAGG AGAAAGTTCT GGCATCCAAT 
CCCATAATGG AGTCTATTGG TAACGCCAAG ACCACCAGGA ATGACAACAG CAGTCGATTT 
GGGAAATACA TTGAGATTGG CTTTGATAAG AAGTATCACA TCATTGGTGC GAACATGAGG 
ACATATCTGC TGGAGAAATC ACGTGTGGTG TTTCAGGCGG ATGAAGAGAG GAATTATCAT 
ATCTTCTATC AGCTCTGTGC ATCGGCTCAC CTCCCGGAGT TTAAAGCCCT GAAACTCGGA 
AAGGCTAATG ATTTCCACTA CACAAAACAG GGCAGGAATC CAGTCATAGA TGGAGTAGAT 
GATGCCAAGG AGATGTCTAC CACCAGGAAC GCCTTCATAC TGCTAGGTAT AAATGAGTCC 
TATCAGATGG GCTTGTTTCA GATATTGGCT TCCATTTTGC ACCTTGGAAA TGTAGACGTG 
AAGGATAGAG ACTCGGACAG CAGTATTATT CCACCAAACA ACGGTCACCT GAGTGTGTTT 
TGTGAGTTGA TGGGAGTAAC ATATCAAGAC ATGTCTCATT GGCTCTGTCA TAAGAAGTTG 
AAGACTGCGA CTGAGACATA CATAAAGCCC ATACCCAAAC TACAGGCTAT AAACGCCCGC 
GACGCTCTCG CCAAACATAT CTACGCCAAA CTCTTCAACT GGATTGTAGA CCACGTGAAC 
AAAGCTCTGC ATTCAACGGT CAAGCAACAC TCTTTCATCG GAGTCTTGGA CATTTACGGG 
TTCGAGACTT TTGAAATCAA CAGTTTTGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTACAGCAAC AGTTCAACAT GCATGTGTTT AAGTTGGAGC AGGAGGAGTA TATGAAAGAG 
CAGATCCCGT GGACTCTCAT TGATTTCTAT GATAATCAGC CATGTATCAA CCTCATAGAG 
GCCAAGATGG GCATCCTGGA CCTGCTGGAT GAAGAGTGCA AGATGCCGAA GGGTTCGGAT 
GACTCCTGGG CTCAGAAACT CTATAACACT CATTTGAAGA CGTGTGCACT TTTTGAAAAG 
CCGCGGATGT CCAATAAAGC TTTCATTATT CAGCACTTTG CAGATAAGGT GGAGTATCAA 
TGTGATGGTT TTCTGGAGAA GAATAAAGAC ACGGTGAATG AGGAGCAGAT AAATGTTTTG 
AAGGCCAGTA AGAAGTTTGA CCTGCTAGTG GAGCTGTTCC ATGATGAGGA GAAAGCCACA 
AGTCCCACCG GTGCTGCCCC TGGCCCTGGA GGACGCACTC GCCTCAGTGT CAAACCAGAC 
AAGGGCAAAT CCAGCCAAGC CAGCAAAGAA CACAAGAAGA CAGTCGGACT GCAGTTCCGG 
AACTCTCTGC AGCTGCTGAT GGAGACACTG AATGCCACTA CACCTCATTA TGTTCGATGC 
ATTAAACCCA ACGATTACAA ACATGCCTTC ACGTTTGACC CCAAGAGGGC AGTGCAGCAA 
CTGCGTGCTT GTGGAGTTTT GGAAACAATC CGCATCTCTG CAGCTGGTTT CCCATCGAGG 
TGGACGTATC AAGAGTTCTT TAGCAGGTAT CGTGTACTGA TGAAACAAAA AGATGTTCTG 
ACGGACAAGA AGATGACCTG TAAGAATGTT CTGGAGAAAC TGGTGCAGGA TCCAGATAAG 
TACCAGTTTG GAAAGACCAA GATTTTTTTC CGCGCAGGTC AGGTTGCATA CCTGGAGAAA 
CTGCGTGCTG ATAAGCTGCG CGCTGCCTGT ATCCGCATCC AGAAGACCAT CCGCTGCTGG 
CTGGCACGCA AGAAGTACCT GCGTATGAAG CATGCTGCCA CGACTATCCA GAGATTCGTC 
CGTGGATACC AGGCCCGCTG TCTTGCCAAA TTCCTGCGTC GCACACGTGC TGCGATCATT 
ATTCAGAAGT ACCAGCGCAT GTACATCCAG AAAACGTGTT ACAAACGCAA GCAGGCTGCC 
GCGCTGGCCA TGCAGTGCAT CCTCAGAGCG TATATGGCCA GACAGCTTTA CAAAGCGCTG 
CTGCGTGAGC ACAAGGCTGT CATTATTCAG AAGATGGTGC GTGGCTGGCT GGCTCGGCAG 
TGGTTCAAGC GCTCGCTCAA AGCCATCGTT TACCTGCAGT GCTGCATTCG GCGAATGCGA 
GCCAAACGAG AACTCAAGAA GCTCAAGATC GAAGCACGCT CGGTCGAACA CTTCAAGAAG 
CTCAACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAGGATAGA CGATCAGAAC 
AAGGAGAACC GTTCCATGAG CGAACGTTTG AACACTCTAG AGACCAGCCA TGCTGTTGAG 
TCAGAGCGTA TGCGCGCCGA AGTGACGCGT CTGCGGGGAG CAGAGGAGGA CGCCAAAAAC 
AACGCCAACA AAGTCACATC TCTTCTGGAG GAGCTGGAAC GACTGCGCAA AGACCTGCAG 
AACACACAGA AGGAGAAGAA AGCCATTGAG GACTGGGCTC AGACCTACCA GGACGAGATG 
GAAAAGATGA TCTCAGAGCT AAAAGAGCAA AACCAGCTGC TGAAGACAGA GAAAAACAAC 
CTAAACCAAC TGATCCAGGA ACAGAGCCAG CAGTGGACAG ACAAAATGCA GAGAGCTCTG 
AAAGAGGAAA CCCAGCAGTT AGAAAACGAT TTAAACGAGG AGCGATCTCG CTACCAAAAT 
CTCCTCACCG AGCACCTCCG ACTAGAGGAG AAATACGACG ATTTGAAAGA AGAGATCACA 
CTCGCTGTGA ATGTTCCAAA ACCTGGTCAC CGGCGCACAG ACTCCACACA CAGCAGCAAC 
GAATCAGAAT GTACCTACAA CTCAGAGTTC GCAGAGTCAG AGGAGGGATC CCGCGTCGGG 
GAGGATGTTT CCAAGTCAAT CCTGGACATG TCTCTGTTCC TGAAGCTGCA GAAGAGAGTT 
TCCGAACTCG AGCAAGAAAA GCAGTCGCTG CAGAACGAAC TGGATCGCAG AGAAGAGCAG 
TTTCAGCGGG CTAGAGCTAG GGATGATGAA GAACATAAGA AAGCTCGTGG TGCAGAGCTG 
GAGTATGAAT CTCTCAAGCG TCAGGAGCTG GAGTCTGAGA ACAAGAAGCT GAAGCATGAT 
CTGAATCAGA TGCGTCAGTC TCTCAGAGGC AGCGTTGAGG CCGGTTCTGG TGCTCCTGGT 
TCTCCTGCGT ACACCGTTCT TCTGGATCAG CTCAATGCCT CCAACGAGGA ACTGGAAGTG 
CGCAAAGAGG AGGTGCTCAT CCTGCGCTCA CAGCTCGTCA GTCAGAAAGA AGCCATGCAG 
CACAAGGATG AGAAGACGAC TATGACTGAA CCTCTGCCAT ACATTGAGGA TGTGCAGAAG 
CTCACTGATG CCAAGGAGAT CTCACAGGCC TACATGGGCC TCAAAGACAC CAACAGGCTG 
TTGGAGTCTC AGTTGCAGGC TCAACGTCGC TCTCATGACA ACGAGGTGGA AGTTTTGCGT 
GGAGAGCTGC ACAGTCTTAA GGAGGAGAAC AACCGTCAGC AGCAGCTGTT GGCGCAGAAC 
CTGCAACTAC CTCCAGAGGC CAGGATTGAA GCCAGTCTAC AGCACGAGAT CACACGCCTC 
ACCAACGAGA ACCTGGAACT TCACTCTGAT GATCCAAAGG AGTATAAAGC CTACAGAATC 
AGCATGTTAC GCAGGATGGT TGATCTTATG GAGCAGTTGG AGAAACAGGA CAAGACCGTT 
CGTAAGCTGA AGAAACAGCT CAAAGTATTT GCTAAGAAGA TCAATGATCT GGAAGGGGGG 
CAGATGGACG TGTCTCCCGG ACAAACGGCT GACGAGCCTG TTCATCCGGT GAACATTCCA 
CGCAGAGAGA AAGATTTCCA GGGCATGCTG GAATACAAGA AAGAGGATGA ACTCAAACTG 
GTCAAAAATA TCATACTTGA GCTGAAGCCT CGTGGTGTTG CAGTGAATCT GATACCAGGT 
CTGCCCGCAT ACATCCTCTT CATGTGTCTG CGCCATGCTG ACTACATCAA CGATGACCAG 
AAAGTGCGAT CTCTACTCAC CTCCGTCATC AACAGCATCA AGAAGATCCT CAAGAAACGA 
GGTGATGACT TTGAGACTGT TTCCTTCTGG TTGGCCAACA CATGTCGTTT TCTGCACTGC 
CTGAAACAGT ACAGTGGTGA TGAGCAATTC ATGAAGCATA ATAGCCCAAA GCAGAACGAA 
CACTGTCTCT CTAACTTTGA CCTGGCAGAG TACCGGCAGG TTCTGAGCGA TCTGGCCATT 
CAGATCTACC AGCAGCTCAT CAAGTGTATG GAAAACATCC TGCAGCCCAT GATCGTCTCT 
GGCATGCTGG AACATGAAAC CATCCAGGGT GTGTCAGGAG TGAAGCCCAC AGGTCTCCGC 
AAGAGGACGT CAAGTATTGC TGATGAGGGC ACGTACACAC TGGACTCCAT CATCCGTCAG 
CTCAACACCT TCCACTCCAT CATGTGTCAC CACGGCACTG ACCCCGAGCT CATCAAACAG 
GTGGTCAAGC AGCAGTTCTA CATCATCGGC GCTGTCACCC TCAACAACCT GCTGCTGCGC 
AAAGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ATAACGTGAG TCAGCTGGAG 
GAATGGCTGA GGGATAAGAA CCTCATGACA TGCGGCGCTA AAGAAACACT GGAGCCGTTG 
ATTCAGGCTG CGCAACTACT GCAGGTGAAG AAGAAAACTG ATGAGGACGC CGAGGCCATC 
TGCTCTATGT GTAACGCCCT CTCCACAGCA CAGATTGTGA AAGTGCTGAA CTTGTACACA 
CCTGTCAATG CATTTGAAGA GCGAGTGTCA GTGTTGTTCA TCAGAACAAT ACAGACTCGT 
TTACGGGATC GAAAGGAGTC GCCTCAGCTT CTGATGGACA CCAAACTCAT CTACCCCGTG 
ACCTTCCCCT TTAACCCCTC CTCCCTTGCC CTGGAAACCA TTCAGATCCC CAGCAGCCTC 
AATCTGGCCT TCCTCACTCG TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293797.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292072.1 Danio rerio myosin VAa (myo5aa), transcript variant X4, mRNA.

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CloneID	ODa43733
Clone ID Related Accession (Same CDS sequence)	XM_009293797.2 , XM_009293797.3
Accession Version	XM_009293797.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	unconventional myosin-Va isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGACGACTAT GACTGAACCT CTGCCATACA TTGAGGATGT GCAGAAGCTC 
ACTGATGCCA AGGAGATCTC ACAGGCCTAC ATGGGCCTCA AAGACACCAA CAGGCTGTTG 
GAGTCTCAGT TGCAGGCTCA ACGTCGCTCT CATGACAACG AGGTGGAAGT TTTGCGTGGA 
GAGCTGCACA GTCTTAAGGA GGAGAACAAC CGTCAGCAGC AGCTGTTGGC GCAGAACCTG 
CAACTACCTC CAGAGGCCAG GATTGAAGCC AGTCTACAGC ACGAGATCAC ACGCCTCACC 
AACGAGAACC TGGAACTTCA CTCTGATGAT CCAAAGGAGT ATAAAGCCTA CAGAATCAGC 
ATGTTACGCA GGATGGTTGA TCTTATGGAG CAGTTGGAGA AACAGGACAA GACCGTTCGT 
AAGCTGAAGA AACAGCTCAA AGTATTTGCT AAGAAGATCA ATGATCTGGA AGGGGGGCAG 
ATGGACGTGT CTCCCGGACA AACGGCTGAC GAGCCTGTTC ATCCGGTGAA CATTCCACGC 
AGAGAGAAAG ATTTCCAGGG CATGCTGGAA TACAAGAAAG AGGATGAACT CAAACTGGTC 
AAAAATATCA TACTTGAGCT GAAGCCTCGT GGTGTTGCAG TGAATCTGAT ACCAGGTCTG 
CCCGCATACA TCCTCTTCAT GTGTCTGCGC CATGCTGACT ACATCAACGA TGACCAGAAA 
GTGCGATCTC TACTCACCTC CGTCATCAAC AGCATCAAGA AGATCCTCAA GAAACGAGGT 
GATGACTTTG AGACTGTTTC CTTCTGGTTG GCCAACACAT GTCGTTTTCT GCACTGCCTG 
AAACAGTACA GTGGTGATGA GCAATTCATG AAGCATAATA GCCCAAAGCA GAACGAACAC 
TGTCTCTCTA ACTTTGACCT GGCAGAGTAC CGGCAGGTTC TGAGCGATCT GGCCATTCAG 
ATCTACCAGC AGCTCATCAA GTGTATGGAA AACATCCTGC AGCCCATGAT CGTCTCTGGC 
ATGCTGGAAC ATGAAACCAT CCAGGGTGTG TCAGGAGTGA AGCCCACAGG TCTCCGCAAG 
AGGACGTCAA GTATTGCTGA TGAGGGCACG TACACACTGG ACTCCATCAT CCGTCAGCTC 
AACACCTTCC ACTCCATCAT GTGTCACCAC GGCACTGACC CCGAGCTCAT CAAACAGGTG 
GTCAAGCAGC AGTTCTACAT CATCGGCGCT GTCACCCTCA ACAACCTGCT GCTGCGCAAA 
GACATGTGCT CCTGGAGCAA GGGCATGCAG ATCAGGTATA ACGTGAGTCA GCTGGAGGAA 
TGGCTGAGGG ATAAGAACCT CATGACATGC GGCGCTAAAG AAACACTGGA GCCGTTGATT 
CAGGCTGCGC AACTACTGCA GGTGAAGAAG AAAACTGATG AGGACGCCGA GGCCATCTGC 
TCTATGTGTA ACGCCCTCTC CACAGCACAG ATTGTGAAAG TGCTGAACTT GTACACACCT 
GTCAATGCAT TTGAAGAGCG AGTGTCAGTG TTGTTCATCA GAACAATACA GACTCGTTTA 
CGGGATCGAA AGGAGTCGCC TCAGCTTCTG ATGGACACCA AACTCATCTA CCCCGTGACC 
TTCCCCTTTA ACCCCTCCTC CCTTGCCCTG GAAACCATTC AGATCCCCAG CAGCCTCAAT 
CTGGCCTTCC TCACTCGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292072.1
CDS	170..5713
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293797.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293797.2

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGACGACTAT GACTGAACCT CTGCCATACA TTGAGGATGT GCAGAAGCTC 
ACTGATGCCA AGGAGATCTC ACAGGCCTAC ATGGGCCTCA AAGACACCAA CAGGCTGTTG 
GAGTCTCAGT TGCAGGCTCA ACGTCGCTCT CATGACAACG AGGTGGAAGT TTTGCGTGGA 
GAGCTGCACA GTCTTAAGGA GGAGAACAAC CGTCAGCAGC AGCTGTTGGC GCAGAACCTG 
CAACTACCTC CAGAGGCCAG GATTGAAGCC AGTCTACAGC ACGAGATCAC ACGCCTCACC 
AACGAGAACC TGGAACTTCA CTCTGATGAT CCAAAGGAGT ATAAAGCCTA CAGAATCAGC 
ATGTTACGCA GGATGGTTGA TCTTATGGAG CAGTTGGAGA AACAGGACAA GACCGTTCGT 
AAGCTGAAGA AACAGCTCAA AGTATTTGCT AAGAAGATCA ATGATCTGGA AGGGGGGCAG 
ATGGACGTGT CTCCCGGACA AACGGCTGAC GAGCCTGTTC ATCCGGTGAA CATTCCACGC 
AGAGAGAAAG ATTTCCAGGG CATGCTGGAA TACAAGAAAG AGGATGAACT CAAACTGGTC 
AAAAATATCA TACTTGAGCT GAAGCCTCGT GGTGTTGCAG TGAATCTGAT ACCAGGTCTG 
CCCGCATACA TCCTCTTCAT GTGTCTGCGC CATGCTGACT ACATCAACGA TGACCAGAAA 
GTGCGATCTC TACTCACCTC CGTCATCAAC AGCATCAAGA AGATCCTCAA GAAACGAGGT 
GATGACTTTG AGACTGTTTC CTTCTGGTTG GCCAACACAT GTCGTTTTCT GCACTGCCTG 
AAACAGTACA GTGGTGATGA GCAATTCATG AAGCATAATA GCCCAAAGCA GAACGAACAC 
TGTCTCTCTA ACTTTGACCT GGCAGAGTAC CGGCAGGTTC TGAGCGATCT GGCCATTCAG 
ATCTACCAGC AGCTCATCAA GTGTATGGAA AACATCCTGC AGCCCATGAT CGTCTCTGGC 
ATGCTGGAAC ATGAAACCAT CCAGGGTGTG TCAGGAGTGA AGCCCACAGG TCTCCGCAAG 
AGGACGTCAA GTATTGCTGA TGAGGGCACG TACACACTGG ACTCCATCAT CCGTCAGCTC 
AACACCTTCC ACTCCATCAT GTGTCACCAC GGCACTGACC CCGAGCTCAT CAAACAGGTG 
GTCAAGCAGC AGTTCTACAT CATCGGCGCT GTCACCCTCA ACAACCTGCT GCTGCGCAAA 
GACATGTGCT CCTGGAGCAA GGGCATGCAG ATCAGGTATA ACGTGAGTCA GCTGGAGGAA 
TGGCTGAGGG ATAAGAACCT CATGACATGC GGCGCTAAAG AAACACTGGA GCCGTTGATT 
CAGGCTGCGC AACTACTGCA GGTGAAGAAG AAAACTGATG AGGACGCCGA GGCCATCTGC 
TCTATGTGTA ACGCCCTCTC CACAGCACAG ATTGTGAAAG TGCTGAACTT GTACACACCT 
GTCAATGCAT TTGAAGAGCG AGTGTCAGTG TTGTTCATCA GAACAATACA GACTCGTTTA 
CGGGATCGAA AGGAGTCGCC TCAGCTTCTG ATGGACACCA AACTCATCTA CCCCGTGACC 
TTCCCCTTTA ACCCCTCCTC CCTTGCCCTG GAAACCATTC AGATCCCCAG CAGCCTCAAT 
CTGGCCTTCC TCACTCGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293797.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292072.1 Danio rerio myosin VAa (myo5aa), transcript variant X4, mRNA.

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CloneID	ODa43733
Clone ID Related Accession (Same CDS sequence)	XM_009293797.2 , XM_009293797.3
Accession Version	XM_009293797.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293797.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGACGACTAT GACTGAACCT CTGCCATACA TTGAGGATGT GCAGAAGCTC 
ACTGATGCCA AGGAGATCTC ACAGGCCTAC ATGGGCCTCA AAGACACCAA CAGGCTGTTG 
GAGTCTCAGT TGCAGGCTCA ACGTCGCTCT CATGACAACG AGGTGGAAGT TTTGCGTGGA 
GAGCTGCACA GTCTTAAGGA GGAGAACAAC CGTCAGCAGC AGCTGTTGGC GCAGAACCTG 
CAACTACCTC CAGAGGCCAG GATTGAAGCC AGTCTACAGC ACGAGATCAC ACGCCTCACC 
AACGAGAACC TGGAACTTCA CTCTGATGAT CCAAAGGAGT ATAAAGCCTA CAGAATCAGC 
ATGTTACGCA GGATGGTTGA TCTTATGGAG CAGTTGGAGA AACAGGACAA GACCGTTCGT 
AAGCTGAAGA AACAGCTCAA AGTATTTGCT AAGAAGATCA ATGATCTGGA AGGGGGGCAG 
ATGGACGTGT CTCCCGGACA AACGGCTGAC GAGCCTGTTC ATCCGGTGAA CATTCCACGC 
AGAGAGAAAG ATTTCCAGGG CATGCTGGAA TACAAGAAAG AGGATGAACT CAAACTGGTC 
AAAAATATCA TACTTGAGCT GAAGCCTCGT GGTGTTGCAG TGAATCTGAT ACCAGGTCTG 
CCCGCATACA TCCTCTTCAT GTGTCTGCGC CATGCTGACT ACATCAACGA TGACCAGAAA 
GTGCGATCTC TACTCACCTC CGTCATCAAC AGCATCAAGA AGATCCTCAA GAAACGAGGT 
GATGACTTTG AGACTGTTTC CTTCTGGTTG GCCAACACAT GTCGTTTTCT GCACTGCCTG 
AAACAGTACA GTGGTGATGA GCAATTCATG AAGCATAATA GCCCAAAGCA GAACGAACAC 
TGTCTCTCTA ACTTTGACCT GGCAGAGTAC CGGCAGGTTC TGAGCGATCT GGCCATTCAG 
ATCTACCAGC AGCTCATCAA GTGTATGGAA AACATCCTGC AGCCCATGAT CGTCTCTGGC 
ATGCTGGAAC ATGAAACCAT CCAGGGTGTG TCAGGAGTGA AGCCCACAGG TCTCCGCAAG 
AGGACGTCAA GTATTGCTGA TGAGGGCACG TACACACTGG ACTCCATCAT CCGTCAGCTC 
AACACCTTCC ACTCCATCAT GTGTCACCAC GGCACTGACC CCGAGCTCAT CAAACAGGTG 
GTCAAGCAGC AGTTCTACAT CATCGGCGCT GTCACCCTCA ACAACCTGCT GCTGCGCAAA 
GACATGTGCT CCTGGAGCAA GGGCATGCAG ATCAGGTATA ACGTGAGTCA GCTGGAGGAA 
TGGCTGAGGG ATAAGAACCT CATGACATGC GGCGCTAAAG AAACACTGGA GCCGTTGATT 
CAGGCTGCGC AACTACTGCA GGTGAAGAAG AAAACTGATG AGGACGCCGA GGCCATCTGC 
TCTATGTGTA ACGCCCTCTC CACAGCACAG ATTGTGAAAG TGCTGAACTT GTACACACCT 
GTCAATGCAT TTGAAGAGCG AGTGTCAGTG TTGTTCATCA GAACAATACA GACTCGTTTA 
CGGGATCGAA AGGAGTCGCC TCAGCTTCTG ATGGACACCA AACTCATCTA CCCCGTGACC 
TTCCCCTTTA ACCCCTCCTC CCTTGCCCTG GAAACCATTC AGATCCCCAG CAGCCTCAAT 
CTGGCCTTCC TCACTCGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292072.1
CDS	166..5709
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293797.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293797.3

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGACGACTAT GACTGAACCT CTGCCATACA TTGAGGATGT GCAGAAGCTC 
ACTGATGCCA AGGAGATCTC ACAGGCCTAC ATGGGCCTCA AAGACACCAA CAGGCTGTTG 
GAGTCTCAGT TGCAGGCTCA ACGTCGCTCT CATGACAACG AGGTGGAAGT TTTGCGTGGA 
GAGCTGCACA GTCTTAAGGA GGAGAACAAC CGTCAGCAGC AGCTGTTGGC GCAGAACCTG 
CAACTACCTC CAGAGGCCAG GATTGAAGCC AGTCTACAGC ACGAGATCAC ACGCCTCACC 
AACGAGAACC TGGAACTTCA CTCTGATGAT CCAAAGGAGT ATAAAGCCTA CAGAATCAGC 
ATGTTACGCA GGATGGTTGA TCTTATGGAG CAGTTGGAGA AACAGGACAA GACCGTTCGT 
AAGCTGAAGA AACAGCTCAA AGTATTTGCT AAGAAGATCA ATGATCTGGA AGGGGGGCAG 
ATGGACGTGT CTCCCGGACA AACGGCTGAC GAGCCTGTTC ATCCGGTGAA CATTCCACGC 
AGAGAGAAAG ATTTCCAGGG CATGCTGGAA TACAAGAAAG AGGATGAACT CAAACTGGTC 
AAAAATATCA TACTTGAGCT GAAGCCTCGT GGTGTTGCAG TGAATCTGAT ACCAGGTCTG 
CCCGCATACA TCCTCTTCAT GTGTCTGCGC CATGCTGACT ACATCAACGA TGACCAGAAA 
GTGCGATCTC TACTCACCTC CGTCATCAAC AGCATCAAGA AGATCCTCAA GAAACGAGGT 
GATGACTTTG AGACTGTTTC CTTCTGGTTG GCCAACACAT GTCGTTTTCT GCACTGCCTG 
AAACAGTACA GTGGTGATGA GCAATTCATG AAGCATAATA GCCCAAAGCA GAACGAACAC 
TGTCTCTCTA ACTTTGACCT GGCAGAGTAC CGGCAGGTTC TGAGCGATCT GGCCATTCAG 
ATCTACCAGC AGCTCATCAA GTGTATGGAA AACATCCTGC AGCCCATGAT CGTCTCTGGC 
ATGCTGGAAC ATGAAACCAT CCAGGGTGTG TCAGGAGTGA AGCCCACAGG TCTCCGCAAG 
AGGACGTCAA GTATTGCTGA TGAGGGCACG TACACACTGG ACTCCATCAT CCGTCAGCTC 
AACACCTTCC ACTCCATCAT GTGTCACCAC GGCACTGACC CCGAGCTCAT CAAACAGGTG 
GTCAAGCAGC AGTTCTACAT CATCGGCGCT GTCACCCTCA ACAACCTGCT GCTGCGCAAA 
GACATGTGCT CCTGGAGCAA GGGCATGCAG ATCAGGTATA ACGTGAGTCA GCTGGAGGAA 
TGGCTGAGGG ATAAGAACCT CATGACATGC GGCGCTAAAG AAACACTGGA GCCGTTGATT 
CAGGCTGCGC AACTACTGCA GGTGAAGAAG AAAACTGATG AGGACGCCGA GGCCATCTGC 
TCTATGTGTA ACGCCCTCTC CACAGCACAG ATTGTGAAAG TGCTGAACTT GTACACACCT 
GTCAATGCAT TTGAAGAGCG AGTGTCAGTG TTGTTCATCA GAACAATACA GACTCGTTTA 
CGGGATCGAA AGGAGTCGCC TCAGCTTCTG ATGGACACCA AACTCATCTA CCCCGTGACC 
TTCCCCTTTA ACCCCTCCTC CCTTGCCCTG GAAACCATTC AGATCCCCAG CAGCCTCAAT 
CTGGCCTTCC TCACTCGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293798.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292073.1 Danio rerio myosin VAa (myo5aa), transcript variant X5, mRNA.

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CloneID	ODa43734
Clone ID Related Accession (Same CDS sequence)	XM_009293798.2 , XM_009293798.3
Accession Version	XM_009293798.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	unconventional myosin-Va isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293798.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGA CGACTATGAC TGAACCTCTG CCATACATTG AGGATGTGCA GAAGCTCACT 
GATGCCAAGG AGATCTCACA GGCCTACATG GGCCTCAAAG ACACCAACAG GCTGTTGGAG 
TCTCAGTTGC AGGCTCAACG TCGCTCTCAT GACAACGAGG TGGAAGTTTT GCGTGGAGAG 
CTGCACAGTC TTAAGGAGGA GAACAACCGT CAGCAGCAGC TGTTGGCGCA GAACCTGCAA 
CTACCTCCAG AGGCCAGGAT TGAAGCCAGT CTACAGCACG AGATCACACG CCTCACCAAC 
GAGAACCTGG AACTTCACTC TGATGATCCA AAGGAGTATA AAGCCTACAG AATCAGCATG 
TTACGCAGGA TGGTTGATCT TATGGAGCAG TTGGAGAAAC AGGACAAGAC CGTTCGTAAG 
CTGAAGAAAC AGCTCAAAGT ATTTGCTAAG AAGATCAATG ATCTGGAAGG GGGGCAGATG 
GACGTGTCTC CCGGACAAAC GGCTGACGAG CCTGTTCATC CGGTGAACAT TCCACGCAGA 
GAGAAAGATT TCCAGGGCAT GCTGGAATAC AAGAAAGAGG ATGAACTCAA ACTGGTCAAA 
AATATCATAC TTGAGCTGAA GCCTCGTGGT GTTGCAGTGA ATCTGATACC AGGTCTGCCC 
GCATACATCC TCTTCATGTG TCTGCGCCAT GCTGACTACA TCAACGATGA CCAGAAAGTG 
CGATCTCTAC TCACCTCCGT CATCAACAGC ATCAAGAAGA TCCTCAAGAA ACGAGGTGAT 
GACTTTGAGA CTGTTTCCTT CTGGTTGGCC AACACATGTC GTTTTCTGCA CTGCCTGAAA 
CAGTACAGTG GTGATGAGCA ATTCATGAAG CATAATAGCC CAAAGCAGAA CGAACACTGT 
CTCTCTAACT TTGACCTGGC AGAGTACCGG CAGGTTCTGA GCGATCTGGC CATTCAGATC 
TACCAGCAGC TCATCAAGTG TATGGAAAAC ATCCTGCAGC CCATGATCGT CTCTGGCATG 
CTGGAACATG AAACCATCCA GGGTGTGTCA GGAGTGAAGC CCACAGGTCT CCGCAAGAGG 
ACGTCAAGTA TTGCTGATGA GGGCACGTAC ACACTGGACT CCATCATCCG TCAGCTCAAC 
ACCTTCCACT CCATCATGTG TCACCACGGC ACTGACCCCG AGCTCATCAA ACAGGTGGTC 
AAGCAGCAGT TCTACATCAT CGGCGCTGTC ACCCTCAACA ACCTGCTGCT GCGCAAAGAC 
ATGTGCTCCT GGAGCAAGGG CATGCAGATC AGGTATAACG TGAGTCAGCT GGAGGAATGG 
CTGAGGGATA AGAACCTCAT GACATGCGGC GCTAAAGAAA CACTGGAGCC GTTGATTCAG 
GCTGCGCAAC TACTGCAGGT GAAGAAGAAA ACTGATGAGG ACGCCGAGGC CATCTGCTCT 
ATGTGTAACG CCCTCTCCAC AGCACAGATT GTGAAAGTGC TGAACTTGTA CACACCTGTC 
AATGCATTTG AAGAGCGAGT GTCAGTGTTG TTCATCAGAA CAATACAGAC TCGTTTACGG 
GATCGAAAGG AGTCGCCTCA GCTTCTGATG GACACCAAAC TCATCTACCC CGTGACCTTC 
CCCTTTAACC CCTCCTCCCT TGCCCTGGAA ACCATTCAGA TCCCCAGCAG CCTCAATCTG 
GCCTTCCTCA CTCGTGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292073.1
CDS	170..5710
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293798.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293798.2

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGA CGACTATGAC TGAACCTCTG CCATACATTG AGGATGTGCA GAAGCTCACT 
GATGCCAAGG AGATCTCACA GGCCTACATG GGCCTCAAAG ACACCAACAG GCTGTTGGAG 
TCTCAGTTGC AGGCTCAACG TCGCTCTCAT GACAACGAGG TGGAAGTTTT GCGTGGAGAG 
CTGCACAGTC TTAAGGAGGA GAACAACCGT CAGCAGCAGC TGTTGGCGCA GAACCTGCAA 
CTACCTCCAG AGGCCAGGAT TGAAGCCAGT CTACAGCACG AGATCACACG CCTCACCAAC 
GAGAACCTGG AACTTCACTC TGATGATCCA AAGGAGTATA AAGCCTACAG AATCAGCATG 
TTACGCAGGA TGGTTGATCT TATGGAGCAG TTGGAGAAAC AGGACAAGAC CGTTCGTAAG 
CTGAAGAAAC AGCTCAAAGT ATTTGCTAAG AAGATCAATG ATCTGGAAGG GGGGCAGATG 
GACGTGTCTC CCGGACAAAC GGCTGACGAG CCTGTTCATC CGGTGAACAT TCCACGCAGA 
GAGAAAGATT TCCAGGGCAT GCTGGAATAC AAGAAAGAGG ATGAACTCAA ACTGGTCAAA 
AATATCATAC TTGAGCTGAA GCCTCGTGGT GTTGCAGTGA ATCTGATACC AGGTCTGCCC 
GCATACATCC TCTTCATGTG TCTGCGCCAT GCTGACTACA TCAACGATGA CCAGAAAGTG 
CGATCTCTAC TCACCTCCGT CATCAACAGC ATCAAGAAGA TCCTCAAGAA ACGAGGTGAT 
GACTTTGAGA CTGTTTCCTT CTGGTTGGCC AACACATGTC GTTTTCTGCA CTGCCTGAAA 
CAGTACAGTG GTGATGAGCA ATTCATGAAG CATAATAGCC CAAAGCAGAA CGAACACTGT 
CTCTCTAACT TTGACCTGGC AGAGTACCGG CAGGTTCTGA GCGATCTGGC CATTCAGATC 
TACCAGCAGC TCATCAAGTG TATGGAAAAC ATCCTGCAGC CCATGATCGT CTCTGGCATG 
CTGGAACATG AAACCATCCA GGGTGTGTCA GGAGTGAAGC CCACAGGTCT CCGCAAGAGG 
ACGTCAAGTA TTGCTGATGA GGGCACGTAC ACACTGGACT CCATCATCCG TCAGCTCAAC 
ACCTTCCACT CCATCATGTG TCACCACGGC ACTGACCCCG AGCTCATCAA ACAGGTGGTC 
AAGCAGCAGT TCTACATCAT CGGCGCTGTC ACCCTCAACA ACCTGCTGCT GCGCAAAGAC 
ATGTGCTCCT GGAGCAAGGG CATGCAGATC AGGTATAACG TGAGTCAGCT GGAGGAATGG 
CTGAGGGATA AGAACCTCAT GACATGCGGC GCTAAAGAAA CACTGGAGCC GTTGATTCAG 
GCTGCGCAAC TACTGCAGGT GAAGAAGAAA ACTGATGAGG ACGCCGAGGC CATCTGCTCT 
ATGTGTAACG CCCTCTCCAC AGCACAGATT GTGAAAGTGC TGAACTTGTA CACACCTGTC 
AATGCATTTG AAGAGCGAGT GTCAGTGTTG TTCATCAGAA CAATACAGAC TCGTTTACGG 
GATCGAAAGG AGTCGCCTCA GCTTCTGATG GACACCAAAC TCATCTACCC CGTGACCTTC 
CCCTTTAACC CCTCCTCCCT TGCCCTGGAA ACCATTCAGA TCCCCAGCAG CCTCAATCTG 
GCCTTCCTCA CTCGTGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293798.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292073.1 Danio rerio myosin VAa (myo5aa), transcript variant X5, mRNA.

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CloneID	ODa43734
Clone ID Related Accession (Same CDS sequence)	XM_009293798.2 , XM_009293798.3
Accession Version	XM_009293798.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293798.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGA CGACTATGAC TGAACCTCTG CCATACATTG AGGATGTGCA GAAGCTCACT 
GATGCCAAGG AGATCTCACA GGCCTACATG GGCCTCAAAG ACACCAACAG GCTGTTGGAG 
TCTCAGTTGC AGGCTCAACG TCGCTCTCAT GACAACGAGG TGGAAGTTTT GCGTGGAGAG 
CTGCACAGTC TTAAGGAGGA GAACAACCGT CAGCAGCAGC TGTTGGCGCA GAACCTGCAA 
CTACCTCCAG AGGCCAGGAT TGAAGCCAGT CTACAGCACG AGATCACACG CCTCACCAAC 
GAGAACCTGG AACTTCACTC TGATGATCCA AAGGAGTATA AAGCCTACAG AATCAGCATG 
TTACGCAGGA TGGTTGATCT TATGGAGCAG TTGGAGAAAC AGGACAAGAC CGTTCGTAAG 
CTGAAGAAAC AGCTCAAAGT ATTTGCTAAG AAGATCAATG ATCTGGAAGG GGGGCAGATG 
GACGTGTCTC CCGGACAAAC GGCTGACGAG CCTGTTCATC CGGTGAACAT TCCACGCAGA 
GAGAAAGATT TCCAGGGCAT GCTGGAATAC AAGAAAGAGG ATGAACTCAA ACTGGTCAAA 
AATATCATAC TTGAGCTGAA GCCTCGTGGT GTTGCAGTGA ATCTGATACC AGGTCTGCCC 
GCATACATCC TCTTCATGTG TCTGCGCCAT GCTGACTACA TCAACGATGA CCAGAAAGTG 
CGATCTCTAC TCACCTCCGT CATCAACAGC ATCAAGAAGA TCCTCAAGAA ACGAGGTGAT 
GACTTTGAGA CTGTTTCCTT CTGGTTGGCC AACACATGTC GTTTTCTGCA CTGCCTGAAA 
CAGTACAGTG GTGATGAGCA ATTCATGAAG CATAATAGCC CAAAGCAGAA CGAACACTGT 
CTCTCTAACT TTGACCTGGC AGAGTACCGG CAGGTTCTGA GCGATCTGGC CATTCAGATC 
TACCAGCAGC TCATCAAGTG TATGGAAAAC ATCCTGCAGC CCATGATCGT CTCTGGCATG 
CTGGAACATG AAACCATCCA GGGTGTGTCA GGAGTGAAGC CCACAGGTCT CCGCAAGAGG 
ACGTCAAGTA TTGCTGATGA GGGCACGTAC ACACTGGACT CCATCATCCG TCAGCTCAAC 
ACCTTCCACT CCATCATGTG TCACCACGGC ACTGACCCCG AGCTCATCAA ACAGGTGGTC 
AAGCAGCAGT TCTACATCAT CGGCGCTGTC ACCCTCAACA ACCTGCTGCT GCGCAAAGAC 
ATGTGCTCCT GGAGCAAGGG CATGCAGATC AGGTATAACG TGAGTCAGCT GGAGGAATGG 
CTGAGGGATA AGAACCTCAT GACATGCGGC GCTAAAGAAA CACTGGAGCC GTTGATTCAG 
GCTGCGCAAC TACTGCAGGT GAAGAAGAAA ACTGATGAGG ACGCCGAGGC CATCTGCTCT 
ATGTGTAACG CCCTCTCCAC AGCACAGATT GTGAAAGTGC TGAACTTGTA CACACCTGTC 
AATGCATTTG AAGAGCGAGT GTCAGTGTTG TTCATCAGAA CAATACAGAC TCGTTTACGG 
GATCGAAAGG AGTCGCCTCA GCTTCTGATG GACACCAAAC TCATCTACCC CGTGACCTTC 
CCCTTTAACC CCTCCTCCCT TGCCCTGGAA ACCATTCAGA TCCCCAGCAG CCTCAATCTG 
GCCTTCCTCA CTCGTGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292073.1
CDS	166..5706
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293798.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293798.3

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGTT TGACCTGCTA GTGGAGCTGT TCCATGATGA GGAGAAAGCC 
ACAAGTCCCA CCGGTGCTGC CCCTGGCCCT GGAGGACGCA CTCGCCTCAG TGTCAAACCA 
GACAAGGGCA AATCCAGCCA AGCCAGCAAA GAACACAAGA AGACAGTCGG ACTGCAGTTC 
CGGAACTCTC TGCAGCTGCT GATGGAGACA CTGAATGCCA CTACACCTCA TTATGTTCGA 
TGCATTAAAC CCAACGATTA CAAACATGCC TTCACGTTTG ACCCCAAGAG GGCAGTGCAG 
CAACTGCGTG CTTGTGGAGT TTTGGAAACA ATCCGCATCT CTGCAGCTGG TTTCCCATCG 
AGGTGGACGT ATCAAGAGTT CTTTAGCAGG TATCGTGTAC TGATGAAACA AAAAGATGTT 
CTGACGGACA AGAAGATGAC CTGTAAGAAT GTTCTGGAGA AACTGGTGCA GGATCCAGAT 
AAGTACCAGT TTGGAAAGAC CAAGATTTTT TTCCGCGCAG GTCAGGTTGC ATACCTGGAG 
AAACTGCGTG CTGATAAGCT GCGCGCTGCC TGTATCCGCA TCCAGAAGAC CATCCGCTGC 
TGGCTGGCAC GCAAGAAGTA CCTGCGTATG AAGCATGCTG CCACGACTAT CCAGAGATTC 
GTCCGTGGAT ACCAGGCCCG CTGTCTTGCC AAATTCCTGC GTCGCACACG TGCTGCGATC 
ATTATTCAGA AGTACCAGCG CATGTACATC CAGAAAACGT GTTACAAACG CAAGCAGGCT 
GCCGCGCTGG CCATGCAGTG CATCCTCAGA GCGTATATGG CCAGACAGCT TTACAAAGCG 
CTGCTGCGTG AGCACAAGGC TGTCATTATT CAGAAGATGG TGCGTGGCTG GCTGGCTCGG 
CAGTGGTTCA AGCGCTCGCT CAAAGCCATC GTTTACCTGC AGTGCTGCAT TCGGCGAATG 
CGAGCCAAAC GAGAACTCAA GAAGCTCAAG ATCGAAGCAC GCTCGGTCGA ACACTTCAAG 
AAGCTCAACA TCGGCATGGA GAACAAGATC ATGCAGCTGC AGCGCAGGAT AGACGATCAG 
AACAAGGAGA ACCGTTCCAT GAGCGAACGT TTGAACACTC TAGAGACCAG CCATGCTGTT 
GAGTCAGAGC GTATGCGCGC CGAAGTGACG CGTCTGCGGG GAGCAGAGGA GGACGCCAAA 
AACAACGCCA ACAAAGTCAC ATCTCTTCTG GAGGAGCTGG AACGACTGCG CAAAGACCTG 
CAGAACACAC AGAAGGAGAA GAAAGCCATT GAGGACTGGG CTCAGACCTA CCAGGACGAG 
ATGGAAAAGA TGATCTCAGA GCTAAAAGAG CAAAACCAGC TGCTGAAGAC AGAGAAAAAC 
AACCTAAACC AACTGATCCA GGAACAGAGC CAGCAGTGGA CAGACAAAAT GCAGAGAGCT 
CTGAAAGAGG AAACCCAGCA GTTAGAAAAC GATTTAAACG AGGAGCGATC TCGCTACCAA 
AATCTCCTCA CCGAGCACCT CCGACTAGAG GAGAAATACG ACGATTTGAA AGAAGAGATC 
ACACTCGCTG TGAATGTTCC AAAACCTGGT CACCGGCGCA CAGACTCCAC ACACAGCAGC 
AACGAATCAG AATGTACCTA CAACTCAGAG TTCGCAGAGT CAGAGGAGGG ATCCCGCGTC 
GGGGAGGATG TTTCCAAGTC AATCCTGGAC ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA 
GTTTCCGAAC TCGAGCAAGA AAAGCAGTCG CTGCAGAACG AACTGGATCG CAGAGAAGAG 
CAGTTTCAGC GGGCTAGAGC TAGGGATGAT GAAGAACATA AGAAAGCTCG TGGTGCAGAG 
CTGGAGTATG AATCTCTCAA GCGTCAGGAG CTGGAGTCTG AGAACAAGAA GCTGAAGCAT 
GATCTGAATC AGATGCGTCA GTCTCTCAGA GGCAGCGTTG AGGCCGGTTC TGGTGCTCCT 
GGTTCTCCTG CGTACACCGT TCTTCTGGAT CAGCTCAATG CCTCCAACGA GGAACTGGAA 
GTGCGCAAAG AGGAGGTGCT CATCCTGCGC TCACAGCTCG TCAGTCAGAA AGAAGCCATG 
CAGCACAAGA CGACTATGAC TGAACCTCTG CCATACATTG AGGATGTGCA GAAGCTCACT 
GATGCCAAGG AGATCTCACA GGCCTACATG GGCCTCAAAG ACACCAACAG GCTGTTGGAG 
TCTCAGTTGC AGGCTCAACG TCGCTCTCAT GACAACGAGG TGGAAGTTTT GCGTGGAGAG 
CTGCACAGTC TTAAGGAGGA GAACAACCGT CAGCAGCAGC TGTTGGCGCA GAACCTGCAA 
CTACCTCCAG AGGCCAGGAT TGAAGCCAGT CTACAGCACG AGATCACACG CCTCACCAAC 
GAGAACCTGG AACTTCACTC TGATGATCCA AAGGAGTATA AAGCCTACAG AATCAGCATG 
TTACGCAGGA TGGTTGATCT TATGGAGCAG TTGGAGAAAC AGGACAAGAC CGTTCGTAAG 
CTGAAGAAAC AGCTCAAAGT ATTTGCTAAG AAGATCAATG ATCTGGAAGG GGGGCAGATG 
GACGTGTCTC CCGGACAAAC GGCTGACGAG CCTGTTCATC CGGTGAACAT TCCACGCAGA 
GAGAAAGATT TCCAGGGCAT GCTGGAATAC AAGAAAGAGG ATGAACTCAA ACTGGTCAAA 
AATATCATAC TTGAGCTGAA GCCTCGTGGT GTTGCAGTGA ATCTGATACC AGGTCTGCCC 
GCATACATCC TCTTCATGTG TCTGCGCCAT GCTGACTACA TCAACGATGA CCAGAAAGTG 
CGATCTCTAC TCACCTCCGT CATCAACAGC ATCAAGAAGA TCCTCAAGAA ACGAGGTGAT 
GACTTTGAGA CTGTTTCCTT CTGGTTGGCC AACACATGTC GTTTTCTGCA CTGCCTGAAA 
CAGTACAGTG GTGATGAGCA ATTCATGAAG CATAATAGCC CAAAGCAGAA CGAACACTGT 
CTCTCTAACT TTGACCTGGC AGAGTACCGG CAGGTTCTGA GCGATCTGGC CATTCAGATC 
TACCAGCAGC TCATCAAGTG TATGGAAAAC ATCCTGCAGC CCATGATCGT CTCTGGCATG 
CTGGAACATG AAACCATCCA GGGTGTGTCA GGAGTGAAGC CCACAGGTCT CCGCAAGAGG 
ACGTCAAGTA TTGCTGATGA GGGCACGTAC ACACTGGACT CCATCATCCG TCAGCTCAAC 
ACCTTCCACT CCATCATGTG TCACCACGGC ACTGACCCCG AGCTCATCAA ACAGGTGGTC 
AAGCAGCAGT TCTACATCAT CGGCGCTGTC ACCCTCAACA ACCTGCTGCT GCGCAAAGAC 
ATGTGCTCCT GGAGCAAGGG CATGCAGATC AGGTATAACG TGAGTCAGCT GGAGGAATGG 
CTGAGGGATA AGAACCTCAT GACATGCGGC GCTAAAGAAA CACTGGAGCC GTTGATTCAG 
GCTGCGCAAC TACTGCAGGT GAAGAAGAAA ACTGATGAGG ACGCCGAGGC CATCTGCTCT 
ATGTGTAACG CCCTCTCCAC AGCACAGATT GTGAAAGTGC TGAACTTGTA CACACCTGTC 
AATGCATTTG AAGAGCGAGT GTCAGTGTTG TTCATCAGAA CAATACAGAC TCGTTTACGG 
GATCGAAAGG AGTCGCCTCA GCTTCTGATG GACACCAAAC TCATCTACCC CGTGACCTTC 
CCCTTTAACC CCTCCTCCCT TGCCCTGGAA ACCATTCAGA TCCCCAGCAG CCTCAATCTG 
GCCTTCCTCA CTCGTGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293799.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292074.1 Danio rerio myosin VAa (myo5aa), transcript variant X6, mRNA.

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CloneID	ODa43735
Clone ID Related Accession (Same CDS sequence)	XM_009293799.2
Accession Version	XM_009293799.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	unconventional myosin-Va isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGT CTGAATTATA TACAAAGTAT GCACGGGTCT GGATCCCGGA TGACGAGGAG 
GTTTGGAGAT CAGCAGAGCT CACTAAAGAC TACAGACAAG GAGACGGAGT ACTGCAACTA 
CAGCTGGAGG ATGGAAAGGA CCTGGAGTTT AAATTGGACC CCAAGACGAA CAACTTGCCA 
CACCTGCGTA ACCCGGATAT CCTGGTGGGA GAAAATGACC TGACTGCTCT CAGCTACCTT 
CACGAACCTG CTGTGTTACA CAACCTGAAA GTACGCTTCA TCGACTCCAA GCTCATCTAC 
ACATACTGCG GTATTGTTCT AGTCGCTATA AACCCTTATG AGACTCTTCC CATCTATGGA 
GCGGACATCA TTAACGCATA CAGTGGGCAG AACATGGGCG ACATGGACCC TCACATCTTC 
GCAGTGGCTG AAGAAGCGTA TAAGCAGATG GCCAGGGATG AGAGGAATCA GTCTATTATT 
GTAAGTGGAG AATCTGGAGC GGGAAAAACG GTTTCGGCCA AATACGCCAT GCGTTACTTT 
GCCACAGTCA GTGGATCTGC CAGCGAAGCC AACGTGGAGG AGAAAGTTCT GGCATCCAAT 
CCCATAATGG AGTCTATTGG TAACGCCAAG ACCACCAGGA ATGACAACAG CAGTCGATTT 
GGGAAATACA TTGAGATTGG CTTTGATAAG AAGTATCACA TCATTGGTGC GAACATGAGG 
ACATATCTGC TGGAGAAATC ACGTGTGGTG TTTCAGGCGG ATGAAGAGAG GAATTATCAT 
ATCTTCTATC AGCTCTGTGC ATCGGCTCAC CTCCCGGAGT TTAAAGCCCT GAAACTCGGA 
AAGGCTAATG ATTTCCACTA CACAAAACAG GGCAGGAATC CAGTCATAGA TGGAGTAGAT 
GATGCCAAGG AGATGTCTAC CACCAGGAAC GCCTTCATAC TGCTAGGTAT AAATGAGTCC 
TATCAGATGG GCTTGTTTCA GATATTGGCT TCCATTTTGC ACCTTGGAAA TGTAGACGTG 
AAGGATAGAG ACTCGGACAG CAGTATTATT CCACCAAACA ACGGTCACCT GAGTGTGTTT 
TGTGAGTTGA TGGGAGTAAC ATATCAAGAC ATGTCTCATT GGCTCTGTCA TAAGAAGTTG 
AAGACTGCGA CTGAGACATA CATAAAGCCC ATACCCAAAC TACAGGCTAT AAACGCCCGC 
GACGCTCTCG CCAAACATAT CTACGCCAAA CTCTTCAACT GGATTGTAGA CCACGTGAAC 
AAAGCTCTGC ATTCAACGGT CAAGCAACAC TCTTTCATCG GAGTCTTGGA CATTTACGGG 
TTCGAGACTT TTGAAATCAA CAGTTTTGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTACAGCAAC AGTTCAACAT GCATGTGTTT AAGTTGGAGC AGGAGGAGTA TATGAAAGAG 
CAGATCCCGT GGACTCTCAT TGATTTCTAT GATAATCAGC CATGTATCAA CCTCATAGAG 
GCCAAGATGG GCATCCTGGA CCTGCTGGAT GAAGAGTGCA AGATGCCGAA GGGTTCGGAT 
GACTCCTGGG CTCAGAAACT CTATAACACT CATTTGAAGA CGTGTGCACT TTTTGAAAAG 
CCGCGGATGT CCAATAAAGC TTTCATTATT CAGCACTTTG CAGATAAGGT GGAGTATCAA 
TGTGATGGTT TTCTGGAGAA GAATAAAGAC ACGGTGAATG AGGAGCAGAT AAATGTTTTG 
AAGGCCAGTA AGAAGTTTGA CCTGCTAGTG GAGCTGTTCC ATGATGAGGA GAAAGCCACA 
AGTCCCACCG GTGCTGCCCC TGGCCCTGGA GGACGCACTC GCCTCAGTGT CAAACCAGAC 
AAGGGCAAAT CCAGCCAAGC CAGCAAAGAA CACAAGAAGA CAGTCGGACT GCAGTTCCGG 
AACTCTCTGC AGCTGCTGAT GGAGACACTG AATGCCACTA CACCTCATTA TGTTCGATGC 
ATTAAACCCA ACGATTACAA ACATGCCTTC ACGTTTGACC CCAAGAGGGC AGTGCAGCAA 
CTGCGTGCTT GTGGAGTTTT GGAAACAATC CGCATCTCTG CAGCTGGTTT CCCATCGAGG 
TGGACGTATC AAGAGTTCTT TAGCAGGTAT CGTGTACTGA TGAAACAAAA AGATGTTCTG 
ACGGACAAGA AGATGACCTG TAAGAATGTT CTGGAGAAAC TGGTGCAGGA TCCAGATAAG 
TACCAGTTTG GAAAGACCAA GATTTTTTTC CGCGCAGGTC AGGTTGCATA CCTGGAGAAA 
CTGCGTGCTG ATAAGCTGCG CGCTGCCTGT ATCCGCATCC AGAAGACCAT CCGCTGCTGG 
CTGGCACGCA AGAAGTACCT GCGTATGAAG CATGCTGCCA CGACTATCCA GAGATTCGTC 
CGTGGATACC AGGCCCGCTG TCTTGCCAAA TTCCTGCGTC GCACACGTGC TGCGATCATT 
ATTCAGAAGT ACCAGCGCAT GTACATCCAG AAAACGTGTT ACAAACGCAA GCAGGCTGCC 
GCGCTGGCCA TGCAGTGCAT CCTCAGAGCG TATATGGCCA GACAGCTTTA CAAAGCGCTG 
CTGCGTGAGC ACAAGGCTGT CATTATTCAG AAGATGGTGC GTGGCTGGCT GGCTCGGCAG 
TGGTTCAAGC GCTCGCTCAA AGCCATCGTT TACCTGCAGT GCTGCATTCG GCGAATGCGA 
GCCAAACGAG AACTCAAGAA GCTCAAGATC GAAGCACGCT CGGTCGAACA CTTCAAGAAG 
CTCAACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAGGATAGA CGATCAGAAC 
AAGGAGAACC GTTCCATGAG CGAACGTTTG AACACTCTAG AGACCAGCCA TGCTGTTGAG 
TCAGAGCGTA TGCGCGCCGA AGTGACGCGT CTGCGGGGAG CAGAGGAGGA CGCCAAAAAC 
AACGCCAACA AAGTCACATC TCTTCTGGAG GAGCTGGAAC GACTGCGCAA AGACCTGCAG 
AACACACAGA AGGAGAAGAA AGCCATTGAG GACTGGGCTC AGACCTACCA GGACGAGATG 
GAAAAGATGA TCTCAGAGCT AAAAGAGCAA AACCAGCTGC TGAAGACAGA GAAAAACAAC 
CTAAACCAAC TGATCCAGGA ACAGAGCCAG CAGTGGACAG ACAAAATGCA GAGAGCTCTG 
AAAGAGGAAA CCCAGCAGTT AGAAAACGAT TTAAACGAGG AGCGATCTCG CTACCAAAAT 
CTCCTCACCG AGCACCTCCG ACTAGAGGAG AAATACGACG ATTTGAAAGA AGAGATCACA 
CTCGCTGTGA ATGTTCCAAA ACCTGGTCAC CGGCGCACAG ACTCCACACA CAGCAGCAAC 
GAATCAGAAT GTACCTACAA CTCAGAGTTC GCAGAGTCAG AGGAGGGATC CCGCGTCGGG 
GAGGATGTTT CCAAGTCAAT CCTGGACATG TCTCTGTTCC TGAAGCTGCA GAAGAGAGTT 
TCCGAACTCG AGCAAGAAAA GCAGTCGCTG CAGAACGAAC TGGATCGCAG AGAAGAGCAG 
TTTCAGCGGG CTAGAGCTAG GGATGATGAA GAACATAAGA AAGCTCGTGG TGCAGAGCTG 
GAGTATGAAT CTCTCAAGCG TCAGGAGCTG GAGTCTGAGA ACAAGAAGCT GAAGCATGAT 
CTGAATCAGA TGCGTCAGTC TCTCAGAGGC AGCGTTGAGG CCGGTTCTGG TGCTCCTGGT 
TCTCCTGCGT ACACCGTTCT TCTGGATCAG CTCAATGCCT CCAACGAGGA ACTGGAAGTG 
CGCAAAGAGG AGGTGCTCAT CCTGCGCTCA CAGCTCGTCA GTCAGAAAGA AGCCATGCAG 
CACAAGACGA CTATGACTGA ACCTCTGCCA TACATTGAGG ATGTGCAGAA GCTCACTGAT 
GCCAAGGAGA TCTCACAGGC CTACATGGGC CTCAAAGACA CCAACAGGCT GTTGGAGTCT 
CAGTTGCAGG CTCAACGTCG CTCTCATGAC AACGAGGTGG AAGTTTTGCG TGGAGAGCTG 
CACAGTCTTA AGGAGGAGAA CAACCGTCAG CAGCAGCTGT TGGCGCAGAA CCTGCAACTA 
CCTCCAGAGG CCAGGATTGA AGCCAGTCTA CAGCACGAGA TCACACGCCT CACCAACGAG 
AACCTGGAAC TTCACTCTGA TGATCCAAAG GAGTATAAAG CCTACAGAAT CAGCATGTTA 
CGCAGGATGG TTGATCTTAT GGAGCAGTTG GAGAAACAGG ACAAGACCGT TCGTAAGCTG 
AAGAAACAGC TCAAAGTATT TGCTAAGAAG ATCAATGATC TGGAAGGGGG GCAGATGGAC 
GTGTCTCCCG GACAAACGGC TGACGAGCCT GTTCATCCGG TGAACATTCC ACGCAGAGAG 
AAAGATTTCC AGGGCATGCT GGAATACAAG AAAGAGGATG AACTCAAACT GGTCAAAAAT 
ATCATACTTG AGCTGAAGCC TCGTGGTGTT GCAGTGAATC TGATACCAGG TCTGCCCGCA 
TACATCCTCT TCATGTGTCT GCGCCATGCT GACTACATCA ACGATGACCA GAAAGTGCGA 
TCTCTACTCA CCTCCGTCAT CAACAGCATC AAGAAGATCC TCAAGAAACG AGGTGATGAC 
TTTGAGACTG TTTCCTTCTG GTTGGCCAAC ACATGTCGTT TTCTGCACTG CCTGAAACAG 
TACAGTGGTG ATGAGCAATT CATGAAGCAT AATAGCCCAA AGCAGAACGA ACACTGTCTC 
TCTAACTTTG ACCTGGCAGA GTACCGGCAG GTTCTGAGCG ATCTGGCCAT TCAGATCTAC 
CAGCAGCTCA TCAAGTGTAT GGAAAACATC CTGCAGCCCA TGATCGTCTC TGGCATGCTG 
GAACATGAAA CCATCCAGGG TGTGTCAGGA GTGAAGCCCA CAGGTCTCCG CAAGAGGACG 
TCAAGTATTG CTGATGAGGG CACGTACACA CTGGACTCCA TCATCCGTCA GCTCAACACC 
TTCCACTCCA TCATGTGTCA CCACGGCACT GACCCCGAGC TCATCAAACA GGTGGTCAAG 
CAGCAGTTCT ACATCATCGG CGCTGTCACC CTCAACAACC TGCTGCTGCG CAAAGACATG 
TGCTCCTGGA GCAAGGGCAT GCAGATCAGG TATAACGTGA GTCAGCTGGA GGAATGGCTG 
AGGGATAAGA ACCTCATGAC ATGCGGCGCT AAAGAAACAC TGGAGCCGTT GATTCAGGCT 
GCGCAACTAC TGCAGGTGAA GAAGAAAACT GATGAGGACG CCGAGGCCAT CTGCTCTATG 
TGTAACGCCC TCTCCACAGC ACAGATTGTG AAAGTGCTGA ACTTGTACAC ACCTGTCAAT 
GCATTTGAAG AGCGAGTGTC AGTGTTGTTC ATCAGAACAA TACAGACTCG TTTACGGGAT 
CGAAAGGAGT CGCCTCAGCT TCTGATGGAC ACCAAACTCA TCTACCCCGT GACCTTCCCC 
TTTAACCCCT CCTCCCTTGC CCTGGAAACC ATTCAGATCC CCAGCAGCCT CAATCTGGCC 
TTCCTCACTC GTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292074.1
CDS	37..5574
Translation	MAASELYTKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLELHSDDPKEYKAYRISMLRRMVDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293799.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293799.2

ORF Insert Sequence:

ATGGCTGCGT CTGAATTATA TACAAAGTAT GCACGGGTCT GGATCCCGGA TGACGAGGAG 
GTTTGGAGAT CAGCAGAGCT CACTAAAGAC TACAGACAAG GAGACGGAGT ACTGCAACTA 
CAGCTGGAGG ATGGAAAGGA CCTGGAGTTT AAATTGGACC CCAAGACGAA CAACTTGCCA 
CACCTGCGTA ACCCGGATAT CCTGGTGGGA GAAAATGACC TGACTGCTCT CAGCTACCTT 
CACGAACCTG CTGTGTTACA CAACCTGAAA GTACGCTTCA TCGACTCCAA GCTCATCTAC 
ACATACTGCG GTATTGTTCT AGTCGCTATA AACCCTTATG AGACTCTTCC CATCTATGGA 
GCGGACATCA TTAACGCATA CAGTGGGCAG AACATGGGCG ACATGGACCC TCACATCTTC 
GCAGTGGCTG AAGAAGCGTA TAAGCAGATG GCCAGGGATG AGAGGAATCA GTCTATTATT 
GTAAGTGGAG AATCTGGAGC GGGAAAAACG GTTTCGGCCA AATACGCCAT GCGTTACTTT 
GCCACAGTCA GTGGATCTGC CAGCGAAGCC AACGTGGAGG AGAAAGTTCT GGCATCCAAT 
CCCATAATGG AGTCTATTGG TAACGCCAAG ACCACCAGGA ATGACAACAG CAGTCGATTT 
GGGAAATACA TTGAGATTGG CTTTGATAAG AAGTATCACA TCATTGGTGC GAACATGAGG 
ACATATCTGC TGGAGAAATC ACGTGTGGTG TTTCAGGCGG ATGAAGAGAG GAATTATCAT 
ATCTTCTATC AGCTCTGTGC ATCGGCTCAC CTCCCGGAGT TTAAAGCCCT GAAACTCGGA 
AAGGCTAATG ATTTCCACTA CACAAAACAG GGCAGGAATC CAGTCATAGA TGGAGTAGAT 
GATGCCAAGG AGATGTCTAC CACCAGGAAC GCCTTCATAC TGCTAGGTAT AAATGAGTCC 
TATCAGATGG GCTTGTTTCA GATATTGGCT TCCATTTTGC ACCTTGGAAA TGTAGACGTG 
AAGGATAGAG ACTCGGACAG CAGTATTATT CCACCAAACA ACGGTCACCT GAGTGTGTTT 
TGTGAGTTGA TGGGAGTAAC ATATCAAGAC ATGTCTCATT GGCTCTGTCA TAAGAAGTTG 
AAGACTGCGA CTGAGACATA CATAAAGCCC ATACCCAAAC TACAGGCTAT AAACGCCCGC 
GACGCTCTCG CCAAACATAT CTACGCCAAA CTCTTCAACT GGATTGTAGA CCACGTGAAC 
AAAGCTCTGC ATTCAACGGT CAAGCAACAC TCTTTCATCG GAGTCTTGGA CATTTACGGG 
TTCGAGACTT TTGAAATCAA CAGTTTTGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTACAGCAAC AGTTCAACAT GCATGTGTTT AAGTTGGAGC AGGAGGAGTA TATGAAAGAG 
CAGATCCCGT GGACTCTCAT TGATTTCTAT GATAATCAGC CATGTATCAA CCTCATAGAG 
GCCAAGATGG GCATCCTGGA CCTGCTGGAT GAAGAGTGCA AGATGCCGAA GGGTTCGGAT 
GACTCCTGGG CTCAGAAACT CTATAACACT CATTTGAAGA CGTGTGCACT TTTTGAAAAG 
CCGCGGATGT CCAATAAAGC TTTCATTATT CAGCACTTTG CAGATAAGGT GGAGTATCAA 
TGTGATGGTT TTCTGGAGAA GAATAAAGAC ACGGTGAATG AGGAGCAGAT AAATGTTTTG 
AAGGCCAGTA AGAAGTTTGA CCTGCTAGTG GAGCTGTTCC ATGATGAGGA GAAAGCCACA 
AGTCCCACCG GTGCTGCCCC TGGCCCTGGA GGACGCACTC GCCTCAGTGT CAAACCAGAC 
AAGGGCAAAT CCAGCCAAGC CAGCAAAGAA CACAAGAAGA CAGTCGGACT GCAGTTCCGG 
AACTCTCTGC AGCTGCTGAT GGAGACACTG AATGCCACTA CACCTCATTA TGTTCGATGC 
ATTAAACCCA ACGATTACAA ACATGCCTTC ACGTTTGACC CCAAGAGGGC AGTGCAGCAA 
CTGCGTGCTT GTGGAGTTTT GGAAACAATC CGCATCTCTG CAGCTGGTTT CCCATCGAGG 
TGGACGTATC AAGAGTTCTT TAGCAGGTAT CGTGTACTGA TGAAACAAAA AGATGTTCTG 
ACGGACAAGA AGATGACCTG TAAGAATGTT CTGGAGAAAC TGGTGCAGGA TCCAGATAAG 
TACCAGTTTG GAAAGACCAA GATTTTTTTC CGCGCAGGTC AGGTTGCATA CCTGGAGAAA 
CTGCGTGCTG ATAAGCTGCG CGCTGCCTGT ATCCGCATCC AGAAGACCAT CCGCTGCTGG 
CTGGCACGCA AGAAGTACCT GCGTATGAAG CATGCTGCCA CGACTATCCA GAGATTCGTC 
CGTGGATACC AGGCCCGCTG TCTTGCCAAA TTCCTGCGTC GCACACGTGC TGCGATCATT 
ATTCAGAAGT ACCAGCGCAT GTACATCCAG AAAACGTGTT ACAAACGCAA GCAGGCTGCC 
GCGCTGGCCA TGCAGTGCAT CCTCAGAGCG TATATGGCCA GACAGCTTTA CAAAGCGCTG 
CTGCGTGAGC ACAAGGCTGT CATTATTCAG AAGATGGTGC GTGGCTGGCT GGCTCGGCAG 
TGGTTCAAGC GCTCGCTCAA AGCCATCGTT TACCTGCAGT GCTGCATTCG GCGAATGCGA 
GCCAAACGAG AACTCAAGAA GCTCAAGATC GAAGCACGCT CGGTCGAACA CTTCAAGAAG 
CTCAACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAGGATAGA CGATCAGAAC 
AAGGAGAACC GTTCCATGAG CGAACGTTTG AACACTCTAG AGACCAGCCA TGCTGTTGAG 
TCAGAGCGTA TGCGCGCCGA AGTGACGCGT CTGCGGGGAG CAGAGGAGGA CGCCAAAAAC 
AACGCCAACA AAGTCACATC TCTTCTGGAG GAGCTGGAAC GACTGCGCAA AGACCTGCAG 
AACACACAGA AGGAGAAGAA AGCCATTGAG GACTGGGCTC AGACCTACCA GGACGAGATG 
GAAAAGATGA TCTCAGAGCT AAAAGAGCAA AACCAGCTGC TGAAGACAGA GAAAAACAAC 
CTAAACCAAC TGATCCAGGA ACAGAGCCAG CAGTGGACAG ACAAAATGCA GAGAGCTCTG 
AAAGAGGAAA CCCAGCAGTT AGAAAACGAT TTAAACGAGG AGCGATCTCG CTACCAAAAT 
CTCCTCACCG AGCACCTCCG ACTAGAGGAG AAATACGACG ATTTGAAAGA AGAGATCACA 
CTCGCTGTGA ATGTTCCAAA ACCTGGTCAC CGGCGCACAG ACTCCACACA CAGCAGCAAC 
GAATCAGAAT GTACCTACAA CTCAGAGTTC GCAGAGTCAG AGGAGGGATC CCGCGTCGGG 
GAGGATGTTT CCAAGTCAAT CCTGGACATG TCTCTGTTCC TGAAGCTGCA GAAGAGAGTT 
TCCGAACTCG AGCAAGAAAA GCAGTCGCTG CAGAACGAAC TGGATCGCAG AGAAGAGCAG 
TTTCAGCGGG CTAGAGCTAG GGATGATGAA GAACATAAGA AAGCTCGTGG TGCAGAGCTG 
GAGTATGAAT CTCTCAAGCG TCAGGAGCTG GAGTCTGAGA ACAAGAAGCT GAAGCATGAT 
CTGAATCAGA TGCGTCAGTC TCTCAGAGGC AGCGTTGAGG CCGGTTCTGG TGCTCCTGGT 
TCTCCTGCGT ACACCGTTCT TCTGGATCAG CTCAATGCCT CCAACGAGGA ACTGGAAGTG 
CGCAAAGAGG AGGTGCTCAT CCTGCGCTCA CAGCTCGTCA GTCAGAAAGA AGCCATGCAG 
CACAAGACGA CTATGACTGA ACCTCTGCCA TACATTGAGG ATGTGCAGAA GCTCACTGAT 
GCCAAGGAGA TCTCACAGGC CTACATGGGC CTCAAAGACA CCAACAGGCT GTTGGAGTCT 
CAGTTGCAGG CTCAACGTCG CTCTCATGAC AACGAGGTGG AAGTTTTGCG TGGAGAGCTG 
CACAGTCTTA AGGAGGAGAA CAACCGTCAG CAGCAGCTGT TGGCGCAGAA CCTGCAACTA 
CCTCCAGAGG CCAGGATTGA AGCCAGTCTA CAGCACGAGA TCACACGCCT CACCAACGAG 
AACCTGGAAC TTCACTCTGA TGATCCAAAG GAGTATAAAG CCTACAGAAT CAGCATGTTA 
CGCAGGATGG TTGATCTTAT GGAGCAGTTG GAGAAACAGG ACAAGACCGT TCGTAAGCTG 
AAGAAACAGC TCAAAGTATT TGCTAAGAAG ATCAATGATC TGGAAGGGGG GCAGATGGAC 
GTGTCTCCCG GACAAACGGC TGACGAGCCT GTTCATCCGG TGAACATTCC ACGCAGAGAG 
AAAGATTTCC AGGGCATGCT GGAATACAAG AAAGAGGATG AACTCAAACT GGTCAAAAAT 
ATCATACTTG AGCTGAAGCC TCGTGGTGTT GCAGTGAATC TGATACCAGG TCTGCCCGCA 
TACATCCTCT TCATGTGTCT GCGCCATGCT GACTACATCA ACGATGACCA GAAAGTGCGA 
TCTCTACTCA CCTCCGTCAT CAACAGCATC AAGAAGATCC TCAAGAAACG AGGTGATGAC 
TTTGAGACTG TTTCCTTCTG GTTGGCCAAC ACATGTCGTT TTCTGCACTG CCTGAAACAG 
TACAGTGGTG ATGAGCAATT CATGAAGCAT AATAGCCCAA AGCAGAACGA ACACTGTCTC 
TCTAACTTTG ACCTGGCAGA GTACCGGCAG GTTCTGAGCG ATCTGGCCAT TCAGATCTAC 
CAGCAGCTCA TCAAGTGTAT GGAAAACATC CTGCAGCCCA TGATCGTCTC TGGCATGCTG 
GAACATGAAA CCATCCAGGG TGTGTCAGGA GTGAAGCCCA CAGGTCTCCG CAAGAGGACG 
TCAAGTATTG CTGATGAGGG CACGTACACA CTGGACTCCA TCATCCGTCA GCTCAACACC 
TTCCACTCCA TCATGTGTCA CCACGGCACT GACCCCGAGC TCATCAAACA GGTGGTCAAG 
CAGCAGTTCT ACATCATCGG CGCTGTCACC CTCAACAACC TGCTGCTGCG CAAAGACATG 
TGCTCCTGGA GCAAGGGCAT GCAGATCAGG TATAACGTGA GTCAGCTGGA GGAATGGCTG 
AGGGATAAGA ACCTCATGAC ATGCGGCGCT AAAGAAACAC TGGAGCCGTT GATTCAGGCT 
GCGCAACTAC TGCAGGTGAA GAAGAAAACT GATGAGGACG CCGAGGCCAT CTGCTCTATG 
TGTAACGCCC TCTCCACAGC ACAGATTGTG AAAGTGCTGA ACTTGTACAC ACCTGTCAAT 
GCATTTGAAG AGCGAGTGTC AGTGTTGTTC ATCAGAACAA TACAGACTCG TTTACGGGAT 
CGAAAGGAGT CGCCTCAGCT TCTGATGGAC ACCAAACTCA TCTACCCCGT GACCTTCCCC 
TTTAACCCCT CCTCCCTTGC CCTGGAAACC ATTCAGATCC CCAGCAGCCT CAATCTGGCC 
TTCCTCACTC GTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293800.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292075.1 Danio rerio myosin VAa (myo5aa), transcript variant X9, mRNA.

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CloneID	ODa43736
Clone ID Related Accession (Same CDS sequence)	XM_009293800.2 , XM_009293800.3
Accession Version	XM_009293800.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	unconventional myosin-Va isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGACGACTAT GACTGAACCT CTGCCATACA TTGAGGATGT GCAGAAGCTC 
ACTGATGCCA AGGAGATCTC ACAGGCCTAC ATGGGCCTCA AAGACACCAA CAGGCTGTTG 
GAGTCTCAGT TGCAGGCTCA ACGTCGCTCT CATGACAACG AGGTGGAAGT TTTGCGTGGA 
GAGCTGCACA GTCTTAAGGA GGAGAACAAC CGTCAGCAGC AGCTGTTGGC GCAGAACCTG 
CAACTACCTC CAGAGGCCAG GATTGAAGCC AGTCTACAGC ACGAGATCAC ACGCCTCACC 
AACGAGAACC TGGATCTTAT GGAGCAGTTG GAGAAACAGG ACAAGACCGT TCGTAAGCTG 
AAGAAACAGC TCAAAGTATT TGCTAAGAAG ATCAATGATC TGGAAGGGGG GCAGATGGAC 
GTGTCTCCCG GACAAACGGC TGACGAGCCT GTTCATCCGG TGAACATTCC ACGCAGAGAG 
AAAGATTTCC AGGGCATGCT GGAATACAAG AAAGAGGATG AACTCAAACT GGTCAAAAAT 
ATCATACTTG AGCTGAAGCC TCGTGGTGTT GCAGTGAATC TGATACCAGG TCTGCCCGCA 
TACATCCTCT TCATGTGTCT GCGCCATGCT GACTACATCA ACGATGACCA GAAAGTGCGA 
TCTCTACTCA CCTCCGTCAT CAACAGCATC AAGAAGATCC TCAAGAAACG AGGTGATGAC 
TTTGAGACTG TTTCCTTCTG GTTGGCCAAC ACATGTCGTT TTCTGCACTG CCTGAAACAG 
TACAGTGGTG ATGAGCAATT CATGAAGCAT AATAGCCCAA AGCAGAACGA ACACTGTCTC 
TCTAACTTTG ACCTGGCAGA GTACCGGCAG GTTCTGAGCG ATCTGGCCAT TCAGATCTAC 
CAGCAGCTCA TCAAGTGTAT GGAAAACATC CTGCAGCCCA TGATCGTCTC TGGCATGCTG 
GAACATGAAA CCATCCAGGG TGTGTCAGGA GTGAAGCCCA CAGGTCTCCG CAAGAGGACG 
TCAAGTATTG CTGATGAGGG CACGTACACA CTGGACTCCA TCATCCGTCA GCTCAACACC 
TTCCACTCCA TCATGTGTCA CCACGGCACT GACCCCGAGC TCATCAAACA GGTGGTCAAG 
CAGCAGTTCT ACATCATCGG CGCTGTCACC CTCAACAACC TGCTGCTGCG CAAAGACATG 
TGCTCCTGGA GCAAGGGCAT GCAGATCAGG TATAACGTGA GTCAGCTGGA GGAATGGCTG 
AGGGATAAGA ACCTCATGAC ATGCGGCGCT AAAGAAACAC TGGAGCCGTT GATTCAGGCT 
GCGCAACTAC TGCAGGTGAA GAAGAAAACT GATGAGGACG CCGAGGCCAT CTGCTCTATG 
TGTAACGCCC TCTCCACAGC ACAGATTGTG AAAGTGCTGA ACTTGTACAC ACCTGTCAAT 
GCATTTGAAG AGCGAGTGTC AGTGTTGTTC ATCAGAACAA TACAGACTCG TTTACGGGAT 
CGAAAGGAGT CGCCTCAGCT TCTGATGGAC ACCAAACTCA TCTACCCCGT GACCTTCCCC 
TTTAACCCCT CCTCCCTTGC CCTGGAAACC ATTCAGATCC CCAGCAGCCT CAATCTGGCC 
TTCCTCACTC GTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292075.1
CDS	169..5646
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293800.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293800.2

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGACGACTAT GACTGAACCT CTGCCATACA TTGAGGATGT GCAGAAGCTC 
ACTGATGCCA AGGAGATCTC ACAGGCCTAC ATGGGCCTCA AAGACACCAA CAGGCTGTTG 
GAGTCTCAGT TGCAGGCTCA ACGTCGCTCT CATGACAACG AGGTGGAAGT TTTGCGTGGA 
GAGCTGCACA GTCTTAAGGA GGAGAACAAC CGTCAGCAGC AGCTGTTGGC GCAGAACCTG 
CAACTACCTC CAGAGGCCAG GATTGAAGCC AGTCTACAGC ACGAGATCAC ACGCCTCACC 
AACGAGAACC TGGATCTTAT GGAGCAGTTG GAGAAACAGG ACAAGACCGT TCGTAAGCTG 
AAGAAACAGC TCAAAGTATT TGCTAAGAAG ATCAATGATC TGGAAGGGGG GCAGATGGAC 
GTGTCTCCCG GACAAACGGC TGACGAGCCT GTTCATCCGG TGAACATTCC ACGCAGAGAG 
AAAGATTTCC AGGGCATGCT GGAATACAAG AAAGAGGATG AACTCAAACT GGTCAAAAAT 
ATCATACTTG AGCTGAAGCC TCGTGGTGTT GCAGTGAATC TGATACCAGG TCTGCCCGCA 
TACATCCTCT TCATGTGTCT GCGCCATGCT GACTACATCA ACGATGACCA GAAAGTGCGA 
TCTCTACTCA CCTCCGTCAT CAACAGCATC AAGAAGATCC TCAAGAAACG AGGTGATGAC 
TTTGAGACTG TTTCCTTCTG GTTGGCCAAC ACATGTCGTT TTCTGCACTG CCTGAAACAG 
TACAGTGGTG ATGAGCAATT CATGAAGCAT AATAGCCCAA AGCAGAACGA ACACTGTCTC 
TCTAACTTTG ACCTGGCAGA GTACCGGCAG GTTCTGAGCG ATCTGGCCAT TCAGATCTAC 
CAGCAGCTCA TCAAGTGTAT GGAAAACATC CTGCAGCCCA TGATCGTCTC TGGCATGCTG 
GAACATGAAA CCATCCAGGG TGTGTCAGGA GTGAAGCCCA CAGGTCTCCG CAAGAGGACG 
TCAAGTATTG CTGATGAGGG CACGTACACA CTGGACTCCA TCATCCGTCA GCTCAACACC 
TTCCACTCCA TCATGTGTCA CCACGGCACT GACCCCGAGC TCATCAAACA GGTGGTCAAG 
CAGCAGTTCT ACATCATCGG CGCTGTCACC CTCAACAACC TGCTGCTGCG CAAAGACATG 
TGCTCCTGGA GCAAGGGCAT GCAGATCAGG TATAACGTGA GTCAGCTGGA GGAATGGCTG 
AGGGATAAGA ACCTCATGAC ATGCGGCGCT AAAGAAACAC TGGAGCCGTT GATTCAGGCT 
GCGCAACTAC TGCAGGTGAA GAAGAAAACT GATGAGGACG CCGAGGCCAT CTGCTCTATG 
TGTAACGCCC TCTCCACAGC ACAGATTGTG AAAGTGCTGA ACTTGTACAC ACCTGTCAAT 
GCATTTGAAG AGCGAGTGTC AGTGTTGTTC ATCAGAACAA TACAGACTCG TTTACGGGAT 
CGAAAGGAGT CGCCTCAGCT TCTGATGGAC ACCAAACTCA TCTACCCCGT GACCTTCCCC 
TTTAACCCCT CCTCCCTTGC CCTGGAAACC ATTCAGATCC CCAGCAGCCT CAATCTGGCC 
TTCCTCACTC GTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5aa ( XM_009293800.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292075.1 Danio rerio myosin VAa (myo5aa), transcript variant X8, mRNA.

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CloneID	ODa43736
Clone ID Related Accession (Same CDS sequence)	XM_009293800.2 , XM_009293800.3
Accession Version	XM_009293800.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	unconventional myosin-Va isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293800.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGACGACTAT GACTGAACCT CTGCCATACA TTGAGGATGT GCAGAAGCTC 
ACTGATGCCA AGGAGATCTC ACAGGCCTAC ATGGGCCTCA AAGACACCAA CAGGCTGTTG 
GAGTCTCAGT TGCAGGCTCA ACGTCGCTCT CATGACAACG AGGTGGAAGT TTTGCGTGGA 
GAGCTGCACA GTCTTAAGGA GGAGAACAAC CGTCAGCAGC AGCTGTTGGC GCAGAACCTG 
CAACTACCTC CAGAGGCCAG GATTGAAGCC AGTCTACAGC ACGAGATCAC ACGCCTCACC 
AACGAGAACC TGGATCTTAT GGAGCAGTTG GAGAAACAGG ACAAGACCGT TCGTAAGCTG 
AAGAAACAGC TCAAAGTATT TGCTAAGAAG ATCAATGATC TGGAAGGGGG GCAGATGGAC 
GTGTCTCCCG GACAAACGGC TGACGAGCCT GTTCATCCGG TGAACATTCC ACGCAGAGAG 
AAAGATTTCC AGGGCATGCT GGAATACAAG AAAGAGGATG AACTCAAACT GGTCAAAAAT 
ATCATACTTG AGCTGAAGCC TCGTGGTGTT GCAGTGAATC TGATACCAGG TCTGCCCGCA 
TACATCCTCT TCATGTGTCT GCGCCATGCT GACTACATCA ACGATGACCA GAAAGTGCGA 
TCTCTACTCA CCTCCGTCAT CAACAGCATC AAGAAGATCC TCAAGAAACG AGGTGATGAC 
TTTGAGACTG TTTCCTTCTG GTTGGCCAAC ACATGTCGTT TTCTGCACTG CCTGAAACAG 
TACAGTGGTG ATGAGCAATT CATGAAGCAT AATAGCCCAA AGCAGAACGA ACACTGTCTC 
TCTAACTTTG ACCTGGCAGA GTACCGGCAG GTTCTGAGCG ATCTGGCCAT TCAGATCTAC 
CAGCAGCTCA TCAAGTGTAT GGAAAACATC CTGCAGCCCA TGATCGTCTC TGGCATGCTG 
GAACATGAAA CCATCCAGGG TGTGTCAGGA GTGAAGCCCA CAGGTCTCCG CAAGAGGACG 
TCAAGTATTG CTGATGAGGG CACGTACACA CTGGACTCCA TCATCCGTCA GCTCAACACC 
TTCCACTCCA TCATGTGTCA CCACGGCACT GACCCCGAGC TCATCAAACA GGTGGTCAAG 
CAGCAGTTCT ACATCATCGG CGCTGTCACC CTCAACAACC TGCTGCTGCG CAAAGACATG 
TGCTCCTGGA GCAAGGGCAT GCAGATCAGG TATAACGTGA GTCAGCTGGA GGAATGGCTG 
AGGGATAAGA ACCTCATGAC ATGCGGCGCT AAAGAAACAC TGGAGCCGTT GATTCAGGCT 
GCGCAACTAC TGCAGGTGAA GAAGAAAACT GATGAGGACG CCGAGGCCAT CTGCTCTATG 
TGTAACGCCC TCTCCACAGC ACAGATTGTG AAAGTGCTGA ACTTGTACAC ACCTGTCAAT 
GCATTTGAAG AGCGAGTGTC AGTGTTGTTC ATCAGAACAA TACAGACTCG TTTACGGGAT 
CGAAAGGAGT CGCCTCAGCT TCTGATGGAC ACCAAACTCA TCTACCCCGT GACCTTCCCC 
TTTAACCCCT CCTCCCTTGC CCTGGAAACC ATTCAGATCC CCAGCAGCCT CAATCTGGCC 
TTCCTCACTC GTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292075.1
CDS	164..5641
Translation	MNQELDFMFIKYARVWIPDDEEVWRSAELTKDYRQGDGVLQLQLEDGKDLEFKLDPKTNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYETLPIYGADIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADEERNYHIFYQLCASAHLPEFKALKLGKANDFHYTKQGRNPVIDGVDDAKEMSTTRNAFILLGINESYQMGLFQILASILHLGNVDVKDRDSDSSIIPPNNGHLSVFCELMGVTYQDMSHWLCHKKLKTATETYIKPIPKLQAINARDALAKHIYAKLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDDSWAQKLYNTHLKTCALFEKPRMSNKAFIIQHFADKVEYQCDGFLEKNKDTVNEEQINVLKASKKFDLLVELFHDEEKATSPTGAAPGPGGRTRLSVKPDKGKSSQASKEHKKTVGLQFRNSLQLLMETLNATTPHYVRCIKPNDYKHAFTFDPKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLTDKKMTCKNVLEKLVQDPDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRCWLARKKYLRMKHAATTIQRFVRGYQARCLAKFLRRTRAAIIIQKYQRMYIQKTCYKRKQAAALAMQCILRAYMARQLYKALLREHKAVIIQKMVRGWLARQWFKRSLKAIVYLQCCIRRMRAKRELKKLKIEARSVEHFKKLNIGMENKIMQLQRRIDDQNKENRSMSERLNTLETSHAVESERMRAEVTRLRGAEEDAKNNANKVTSLLEELERLRKDLQNTQKEKKAIEDWAQTYQDEMEKMISELKEQNQLLKTEKNNLNQLIQEQSQQWTDKMQRALKEETQQLENDLNEERSRYQNLLTEHLRLEEKYDDLKEEITLAVNVPKPGHRRTDSTHSSNESECTYNSEFAESEEGSRVGEDVSKSILDMSLFLKLQKRVSELEQEKQSLQNELDRREEQFQRARARDDEEHKKARGAELEYESLKRQELESENKKLKHDLNQMRQSLRGSVEAGSGAPGSPAYTVLLDQLNASNEELEVRKEEVLILRSQLVSQKEAMQHKTTMTEPLPYIEDVQKLTDAKEISQAYMGLKDTNRLLESQLQAQRRSHDNEVEVLRGELHSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKINDLEGGQMDVSPGQTADEPVHPVNIPRREKDFQGMLEYKKEDELKLVKNIILELKPRGVAVNLIPGLPAYILFMCLRHADYINDDQKVRSLLTSVINSIKKILKKRGDDFETVSFWLANTCRFLHCLKQYSGDEQFMKHNSPKQNEHCLSNFDLAEYRQVLSDLAIQIYQQLIKCMENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNTFHSIMCHHGTDPELIKQVVKQQFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMTCGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALSTAQIVKVLNLYTPVNAFEERVSVLFIRTIQTRLRDRKESPQLLMDTKLIYPVTFPFNPSSLALETIQIPSSLNLAFLTRV

Target ORF information:

RefSeq Version	XM_009293800.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin VAa (myo5aa), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293800.3

ORF Insert Sequence:

ATGAACCAGG AGTTGGATTT CATGTTTATT AAATATGCAC GGGTCTGGAT CCCGGATGAC 
GAGGAGGTTT GGAGATCAGC AGAGCTCACT AAAGACTACA GACAAGGAGA CGGAGTACTG 
CAACTACAGC TGGAGGATGG AAAGGACCTG GAGTTTAAAT TGGACCCCAA GACGAACAAC 
TTGCCACACC TGCGTAACCC GGATATCCTG GTGGGAGAAA ATGACCTGAC TGCTCTCAGC 
TACCTTCACG AACCTGCTGT GTTACACAAC CTGAAAGTAC GCTTCATCGA CTCCAAGCTC 
ATCTACACAT ACTGCGGTAT TGTTCTAGTC GCTATAAACC CTTATGAGAC TCTTCCCATC 
TATGGAGCGG ACATCATTAA CGCATACAGT GGGCAGAACA TGGGCGACAT GGACCCTCAC 
ATCTTCGCAG TGGCTGAAGA AGCGTATAAG CAGATGGCCA GGGATGAGAG GAATCAGTCT 
ATTATTGTAA GTGGAGAATC TGGAGCGGGA AAAACGGTTT CGGCCAAATA CGCCATGCGT 
TACTTTGCCA CAGTCAGTGG ATCTGCCAGC GAAGCCAACG TGGAGGAGAA AGTTCTGGCA 
TCCAATCCCA TAATGGAGTC TATTGGTAAC GCCAAGACCA CCAGGAATGA CAACAGCAGT 
CGATTTGGGA AATACATTGA GATTGGCTTT GATAAGAAGT ATCACATCAT TGGTGCGAAC 
ATGAGGACAT ATCTGCTGGA GAAATCACGT GTGGTGTTTC AGGCGGATGA AGAGAGGAAT 
TATCATATCT TCTATCAGCT CTGTGCATCG GCTCACCTCC CGGAGTTTAA AGCCCTGAAA 
CTCGGAAAGG CTAATGATTT CCACTACACA AAACAGGGCA GGAATCCAGT CATAGATGGA 
GTAGATGATG CCAAGGAGAT GTCTACCACC AGGAACGCCT TCATACTGCT AGGTATAAAT 
GAGTCCTATC AGATGGGCTT GTTTCAGATA TTGGCTTCCA TTTTGCACCT TGGAAATGTA 
GACGTGAAGG ATAGAGACTC GGACAGCAGT ATTATTCCAC CAAACAACGG TCACCTGAGT 
GTGTTTTGTG AGTTGATGGG AGTAACATAT CAAGACATGT CTCATTGGCT CTGTCATAAG 
AAGTTGAAGA CTGCGACTGA GACATACATA AAGCCCATAC CCAAACTACA GGCTATAAAC 
GCCCGCGACG CTCTCGCCAA ACATATCTAC GCCAAACTCT TCAACTGGAT TGTAGACCAC 
GTGAACAAAG CTCTGCATTC AACGGTCAAG CAACACTCTT TCATCGGAGT CTTGGACATT 
TACGGGTTCG AGACTTTTGA AATCAACAGT TTTGAACAGT TCTGTATCAA CTATGCCAAT 
GAGAAACTAC AGCAACAGTT CAACATGCAT GTGTTTAAGT TGGAGCAGGA GGAGTATATG 
AAAGAGCAGA TCCCGTGGAC TCTCATTGAT TTCTATGATA ATCAGCCATG TATCAACCTC 
ATAGAGGCCA AGATGGGCAT CCTGGACCTG CTGGATGAAG AGTGCAAGAT GCCGAAGGGT 
TCGGATGACT CCTGGGCTCA GAAACTCTAT AACACTCATT TGAAGACGTG TGCACTTTTT 
GAAAAGCCGC GGATGTCCAA TAAAGCTTTC ATTATTCAGC ACTTTGCAGA TAAGGTGGAG 
TATCAATGTG ATGGTTTTCT GGAGAAGAAT AAAGACACGG TGAATGAGGA GCAGATAAAT 
GTTTTGAAGG CCAGTAAGAA GTTTGACCTG CTAGTGGAGC TGTTCCATGA TGAGGAGAAA 
GCCACAAGTC CCACCGGTGC TGCCCCTGGC CCTGGAGGAC GCACTCGCCT CAGTGTCAAA 
CCAGACAAGG GCAAATCCAG CCAAGCCAGC AAAGAACACA AGAAGACAGT CGGACTGCAG 
TTCCGGAACT CTCTGCAGCT GCTGATGGAG ACACTGAATG CCACTACACC TCATTATGTT 
CGATGCATTA AACCCAACGA TTACAAACAT GCCTTCACGT TTGACCCCAA GAGGGCAGTG 
CAGCAACTGC GTGCTTGTGG AGTTTTGGAA ACAATCCGCA TCTCTGCAGC TGGTTTCCCA 
TCGAGGTGGA CGTATCAAGA GTTCTTTAGC AGGTATCGTG TACTGATGAA ACAAAAAGAT 
GTTCTGACGG ACAAGAAGAT GACCTGTAAG AATGTTCTGG AGAAACTGGT GCAGGATCCA 
GATAAGTACC AGTTTGGAAA GACCAAGATT TTTTTCCGCG CAGGTCAGGT TGCATACCTG 
GAGAAACTGC GTGCTGATAA GCTGCGCGCT GCCTGTATCC GCATCCAGAA GACCATCCGC 
TGCTGGCTGG CACGCAAGAA GTACCTGCGT ATGAAGCATG CTGCCACGAC TATCCAGAGA 
TTCGTCCGTG GATACCAGGC CCGCTGTCTT GCCAAATTCC TGCGTCGCAC ACGTGCTGCG 
ATCATTATTC AGAAGTACCA GCGCATGTAC ATCCAGAAAA CGTGTTACAA ACGCAAGCAG 
GCTGCCGCGC TGGCCATGCA GTGCATCCTC AGAGCGTATA TGGCCAGACA GCTTTACAAA 
GCGCTGCTGC GTGAGCACAA GGCTGTCATT ATTCAGAAGA TGGTGCGTGG CTGGCTGGCT 
CGGCAGTGGT TCAAGCGCTC GCTCAAAGCC ATCGTTTACC TGCAGTGCTG CATTCGGCGA 
ATGCGAGCCA AACGAGAACT CAAGAAGCTC AAGATCGAAG CACGCTCGGT CGAACACTTC 
AAGAAGCTCA ACATCGGCAT GGAGAACAAG ATCATGCAGC TGCAGCGCAG GATAGACGAT 
CAGAACAAGG AGAACCGTTC CATGAGCGAA CGTTTGAACA CTCTAGAGAC CAGCCATGCT 
GTTGAGTCAG AGCGTATGCG CGCCGAAGTG ACGCGTCTGC GGGGAGCAGA GGAGGACGCC 
AAAAACAACG CCAACAAAGT CACATCTCTT CTGGAGGAGC TGGAACGACT GCGCAAAGAC 
CTGCAGAACA CACAGAAGGA GAAGAAAGCC ATTGAGGACT GGGCTCAGAC CTACCAGGAC 
GAGATGGAAA AGATGATCTC AGAGCTAAAA GAGCAAAACC AGCTGCTGAA GACAGAGAAA 
AACAACCTAA ACCAACTGAT CCAGGAACAG AGCCAGCAGT GGACAGACAA AATGCAGAGA 
GCTCTGAAAG AGGAAACCCA GCAGTTAGAA AACGATTTAA ACGAGGAGCG ATCTCGCTAC 
CAAAATCTCC TCACCGAGCA CCTCCGACTA GAGGAGAAAT ACGACGATTT GAAAGAAGAG 
ATCACACTCG CTGTGAATGT TCCAAAACCT GGTCACCGGC GCACAGACTC CACACACAGC 
AGCAACGAAT CAGAATGTAC CTACAACTCA GAGTTCGCAG AGTCAGAGGA GGGATCCCGC 
GTCGGGGAGG ATGTTTCCAA GTCAATCCTG GACATGTCTC TGTTCCTGAA GCTGCAGAAG 
AGAGTTTCCG AACTCGAGCA AGAAAAGCAG TCGCTGCAGA ACGAACTGGA TCGCAGAGAA 
GAGCAGTTTC AGCGGGCTAG AGCTAGGGAT GATGAAGAAC ATAAGAAAGC TCGTGGTGCA 
GAGCTGGAGT ATGAATCTCT CAAGCGTCAG GAGCTGGAGT CTGAGAACAA GAAGCTGAAG 
CATGATCTGA ATCAGATGCG TCAGTCTCTC AGAGGCAGCG TTGAGGCCGG TTCTGGTGCT 
CCTGGTTCTC CTGCGTACAC CGTTCTTCTG GATCAGCTCA ATGCCTCCAA CGAGGAACTG 
GAAGTGCGCA AAGAGGAGGT GCTCATCCTG CGCTCACAGC TCGTCAGTCA GAAAGAAGCC 
ATGCAGCACA AGACGACTAT GACTGAACCT CTGCCATACA TTGAGGATGT GCAGAAGCTC 
ACTGATGCCA AGGAGATCTC ACAGGCCTAC ATGGGCCTCA AAGACACCAA CAGGCTGTTG 
GAGTCTCAGT TGCAGGCTCA ACGTCGCTCT CATGACAACG AGGTGGAAGT TTTGCGTGGA 
GAGCTGCACA GTCTTAAGGA GGAGAACAAC CGTCAGCAGC AGCTGTTGGC GCAGAACCTG 
CAACTACCTC CAGAGGCCAG GATTGAAGCC AGTCTACAGC ACGAGATCAC ACGCCTCACC 
AACGAGAACC TGGATCTTAT GGAGCAGTTG GAGAAACAGG ACAAGACCGT TCGTAAGCTG 
AAGAAACAGC TCAAAGTATT TGCTAAGAAG ATCAATGATC TGGAAGGGGG GCAGATGGAC 
GTGTCTCCCG GACAAACGGC TGACGAGCCT GTTCATCCGG TGAACATTCC ACGCAGAGAG 
AAAGATTTCC AGGGCATGCT GGAATACAAG AAAGAGGATG AACTCAAACT GGTCAAAAAT 
ATCATACTTG AGCTGAAGCC TCGTGGTGTT GCAGTGAATC TGATACCAGG TCTGCCCGCA 
TACATCCTCT TCATGTGTCT GCGCCATGCT GACTACATCA ACGATGACCA GAAAGTGCGA 
TCTCTACTCA CCTCCGTCAT CAACAGCATC AAGAAGATCC TCAAGAAACG AGGTGATGAC 
TTTGAGACTG TTTCCTTCTG GTTGGCCAAC ACATGTCGTT TTCTGCACTG CCTGAAACAG 
TACAGTGGTG ATGAGCAATT CATGAAGCAT AATAGCCCAA AGCAGAACGA ACACTGTCTC 
TCTAACTTTG ACCTGGCAGA GTACCGGCAG GTTCTGAGCG ATCTGGCCAT TCAGATCTAC 
CAGCAGCTCA TCAAGTGTAT GGAAAACATC CTGCAGCCCA TGATCGTCTC TGGCATGCTG 
GAACATGAAA CCATCCAGGG TGTGTCAGGA GTGAAGCCCA CAGGTCTCCG CAAGAGGACG 
TCAAGTATTG CTGATGAGGG CACGTACACA CTGGACTCCA TCATCCGTCA GCTCAACACC 
TTCCACTCCA TCATGTGTCA CCACGGCACT GACCCCGAGC TCATCAAACA GGTGGTCAAG 
CAGCAGTTCT ACATCATCGG CGCTGTCACC CTCAACAACC TGCTGCTGCG CAAAGACATG 
TGCTCCTGGA GCAAGGGCAT GCAGATCAGG TATAACGTGA GTCAGCTGGA GGAATGGCTG 
AGGGATAAGA ACCTCATGAC ATGCGGCGCT AAAGAAACAC TGGAGCCGTT GATTCAGGCT 
GCGCAACTAC TGCAGGTGAA GAAGAAAACT GATGAGGACG CCGAGGCCAT CTGCTCTATG 
TGTAACGCCC TCTCCACAGC ACAGATTGTG AAAGTGCTGA ACTTGTACAC ACCTGTCAAT 
GCATTTGAAG AGCGAGTGTC AGTGTTGTTC ATCAGAACAA TACAGACTCG TTTACGGGAT 
CGAAAGGAGT CGCCTCAGCT TCTGATGGAC ACCAAACTCA TCTACCCCGT GACCTTCCCC 
TTTAACCCCT CCTCCCTTGC CCTGGAAACC ATTCAGATCC CCAGCAGCCT CAATCTGGCC 
TTCCTCACTC GTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	myo5aa
Entrez Gene ID	562188
Full Name	myosin VAa
Synonyms	myo5a,si:dkey-266j9.1
General protein information	Preferred Names myosin VAa Names myosin-Va unconventional myosin-Va heavy polypeptide 12 myosin VA (heavy polypeptide 12, myoxin) myoxin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

	MYO5A	XP_001084476.1
Canis lupus familiaris (dog)	MYO5A	XP_005638745.1
Mus musculus (house mouse)	Myo5a	NP_034994.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006479	XP_316519.4
Kluyveromyces lactis	KLLA0A03905g	XP_451171.1
Magnaporthe oryzae (rice blast fungus)	MGG_16213	XP_003709659.1
Arabidopsis thaliana (thale cress)	XIC	NP_172349.2
Oryza sativa (rice)	Os06g0488200	NP_001057670.1
Arabidopsis thaliana (thale cress)	XIE	NP_175858.1
Bos taurus (cattle)	MYO5A	XP_005211933.1
Rattus norvegicus (Norway rat)	Myo5a	NP_071514.1
Gallus gallus (chicken)	MYO5A	NP_990631.1
Neurospora crassa	NCU11354	XP_001728528.1
Xenopus tropicalis (tropical clawed frog)	myo5a	XP_004912752.1
Pan troglodytes (chimpanzee)	MYO5A	XP_510412.2
Danio rerio (zebrafish)	myo5aa	XP_005173731.1
Drosophila melanogaster (fruit fly)	didum	NP_724569.1
Caenorhabditis elegans (roundworm)	hum-2	NP_001023886.1
Saccharomyces cerevisiae (baker's yeast)	MYO2	NP_014971.1
Homo sapiens (human)	MYO5A	NP_000250.3
Eremothecium gossypii	AGOS_ADR354W	NP_984450.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

myo5aa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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