MYO5A gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017022227.1	XP_016877716.1	unconventional myosin-Va isoform X7
XM_005254397.3	XP_005254454.1	unconventional myosin-Va isoform X3
XM_011521610.2	XP_011519912.2	unconventional myosin-Va isoform X6
XM_005254397.4	XP_005254454.1	unconventional myosin-Va isoform X3
XM_011521608.3	XP_011519910.2	unconventional myosin-Va isoform X4
XM_017022227.2	XP_016877716.1	unconventional myosin-Va isoform X7
XM_011521612.3	XP_011519914.2	unconventional myosin-Va isoform X9
XM_011521608.2	XP_011519910.2	unconventional myosin-Va isoform X4
XM_011521611.2	XP_011519913.2	unconventional myosin-Va isoform X8
XM_011521612.2	XP_011519914.2	unconventional myosin-Va isoform X9
NM_001142495.1	NP_001135967.1	unconventional myosin-Va isoform 2
XM_011521606.2	XP_011519908.2	unconventional myosin-Va isoform X1
XM_011521610.3	XP_011519912.2	unconventional myosin-Va isoform X6
NM_000259.3	NP_000250.3	unconventional myosin-Va isoform 1
XM_011521607.2	XP_011519909.2	unconventional myosin-Va isoform X2
NM_001142495.2	NP_001135967.2	unconventional myosin-Va isoform 2
XM_011521607.3	XP_011519909.2	unconventional myosin-Va isoform X2
XM_011521609.3	XP_011519911.2	unconventional myosin-Va isoform X5
XM_011521609.2	XP_011519911.2	unconventional myosin-Va isoform X5
XM_011521611.3	XP_011519913.2	unconventional myosin-Va isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu108394	NM_001142495.2 Latest version!	Homo sapiens myosin VA (MYO5A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85429	XM_011521606.2 Latest version!	Homo sapiens myosin VA (MYO5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85430	XM_011521607.3 Latest version!	Homo sapiens myosin VA (MYO5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85431	XM_011521608.3 Latest version!	Homo sapiens myosin VA (MYO5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85432	XM_011521609.3 Latest version!	Homo sapiens myosin VA (MYO5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85433	XM_011521610.3 Latest version!	Homo sapiens myosin VA (MYO5A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85434	XM_017022227.2 Latest version!	Homo sapiens myosin VA (MYO5A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85435	XM_011521611.3 Latest version!	Homo sapiens myosin VA (MYO5A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85436	XM_011521612.3 Latest version!	Homo sapiens myosin VA (MYO5A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu24827	NM_001142495.1 Latest version!	Homo sapiens myosin VA (MYO5A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu24969	NM_000259.3 Latest version!	Homo sapiens myosin VA (MYO5A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu48319	XM_005254397.4 Latest version!	Homo sapiens myosin VA (MYO5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu48319	XM_005254397.3	Homo sapiens myosin VA (MYO5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu85434	XM_017022227.1	Homo sapiens myosin VA (MYO5A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85431	XM_011521608.2	Homo sapiens myosin VA (MYO5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85436	XM_011521612.2	Homo sapiens myosin VA (MYO5A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85433	XM_011521610.2	Homo sapiens myosin VA (MYO5A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85430	XM_011521607.2	Homo sapiens myosin VA (MYO5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85432	XM_011521609.2	Homo sapiens myosin VA (MYO5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85435	XM_011521611.2	Homo sapiens myosin VA (MYO5A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

MYO5A ( NM_001142495.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135967.2 Homo sapiens myosin VA (MYO5A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu108394
Clone ID Related Accession (Same CDS sequence)	NM_001142495.2
Accession Version	NM_001142495.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025917.8, AC018902.8, KF459554.1 and AC010674.11. On Jun 2, 2019 this sequence version replaced NM_001142495.1. Transcript Variant: This variant (2) lacks an in-frame exon in the CDS, resulting in a shorter isoform (2), as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.115604.1, SRR1803616.79370.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGT CGGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCAGAGGAA 
GTCTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT CCTCCTGCTT 
CACCTCGAGG AAGGAAAGGA TTTGGAATAC CATCTAGATC CAAAGACCAA GGAGCTGCCT 
CACTTACGAA ATCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCCCT CAGCTATCTT 
CATGAGCCTG CTGTGCTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACGTATTGTG GTATAGTCCT AGTAGCTATA AATCCCTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA CAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATC 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GCGATACTTT 
GCAACTGTGA GTGGTTCTGC CAGTGAGGCC AATGTGGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG CAGCCGTTTT 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATTGGTGC CAATATGAGA 
ACTTATCTTT TAGAGAAATC CAGAGTGGTA TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AGCTTTGTGC CTCAGCAAAG TTACCTGAAT TTAAAATGCT ACGATTAGGA 
AATGCAGATA ACTTTAATTA CACAAAACAA GGAGGCAGTC CTGTGATTGA AGGAGTGGAT 
GATGCAAAGG AGATGGCACA TACTAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAAATGG GAATTTTCCG AATACTTGCT GGCATCCTTC ACTTAGGCAA TGTTGGATTT 
ACATCCCGAG ATGCAGACAG CTGCACAATA CCTCCCAAGC ATGAACCTCT CTGCATCTTC 
TGTGAACTCA TGGGTGTGGA CTATGAGGAG ATGTGTCACT GGCTCTGCCA TCGGAAACTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGC 
GATGCTTTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TAATGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAACAGCAC TCTTTTATTG GTGTGCTAGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTCTTC AAATTGGAGC AAGAAGAATA TATGAAGGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGTATTAA TCTTATAGAA 
TCAAAACTAG GCATTCTAGA TTTACTGGAT GAGGAATGCA AGATGCCTAA AGGCACAGAT 
GACACCTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT CTTTGAAAAG 
CCTCGTCTAT CAAACAAAGC TTTCATCATC CAACATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGAT TTCTCGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCAACTTCAG CCACCTCCTC AGGGCGCACA CCCCTCACAC GAACTCCTGC AAAGCCCACC 
AAAGGCAGAC CAGGCCAAAT GGCCAAAGAG CACAAGAAAA CAGTGGGGCA TCAGTTCAGA 
AACTCCCTGC ACCTGCTTAT GGAGACACTC AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ATGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATC CGAATCAGTG CGGCCGGTTT CCCCTCACGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAC CGTGTCCTAA TGAAGCAGAA AGATGTGCTG 
AGTGACAGAA AGCAAACATG CAAGAATGTG TTAGAGAAAC TGATACTGGA CAAGGACAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCCGGTC AAGTGGCCTA TCTAGAAAAA 
TTGAGAGCTG ACAAACTGAG AGCTGCCTGC ATCCGGATCC AGAAGACCAT CCGAGGGTGG 
CTGCTGAGAA AGAAGTACCT ACGCATGCGG AAGGCAGCCA TCACCATGCA GAGATACGTG 
CGGGGCTACC AGGCCCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCATC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACTATCGTTC TTCAGTCTTA CTTGCGAGGC TTCTTGGCCA GAAATAGGTA TCGCAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGCGAGTCC GGGGCTGGCT GGCCCGCACA 
CACTACAAGA GGAGCATGCA TGCCATCATC TACCTTCAGT GCTGCTTCAG GCGGATGATG 
GCCAAGCGTG AGCTAAAGAA GCTCAAAATC GAGGCTCGCT CAGTGGAGCG CTATAAGAAG 
CTGCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTTGT GGAGAAACTA ACCAATCTGG AAGGAATATA CAACTCTGAG 
ACTGAGAAAC TACGAAGTGA CTTAGAACGT CTTCAACTAA GTGAAGAGGA AGCGAAAGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCCA AGCTCCGGAA AGACCTGGAG 
CAAACTCGTT CAGAGAAAAA ATGCATTGAG GAACATGCAG ATCGATACAA ACAAGAAACA 
GAGCAGCTGG TATCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAAGA AAAAGAAGCC 
CTCAATCACC GCATCGTGCA GCAGGCTAAG GAGATGACAG AAACTATGGA GAAGAAGTTA 
GTAGAAGAAA CGAAACAACT GGAACTCGAC CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAAGAA AGATATGATG ACCTCAAGGA AGAGATGACC 
CTTATGGTGC ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCCACCCA CAGCAGCAAC 
GAGTCTGAAT ATATCTTTAG CTCTGAAATT GCAGAAATGG AAGACATTCC ATCAAGGACA 
GAGGAACCAA GTGAGAAGAA GGTACCTCTG GACATGTCAT TGTTCCTTAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCCAAGGAA GAAGAAAGAC CACAAATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAATCAG AAAACAAAAA ACTGAAGAAT 
GAGCTAAATG AGTTGCGCAA GGCCCTCAGT GAGAAAAGTG CCCCAGAGGT GACCGCCCCA 
GGTGCACCTG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGCGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCTTAAGG TCTCAACTGG TGAGCCAGAA AGAGGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TACTTTTGGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATACATTG GTTTGAAAGA AACAAATAGG 
CTCCTGGAAT CCCAGCTGCA GTCACAGAAG AGGAGCCATG AGAATGAGGC CGAGGCCCTC 
CGTGGGGAGA TCCAGAGCCT GAAGGAGGAG AACAACCGAC AGCAGCAGCT GCTGGCCCAG 
AACCTGCAGC TGCCCCCAGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACCCGG 
CTGACCAACG AAAACTTGGA TTTGATGGAA CAACTTGAAA AACAGGATAA GACGGTCCGT 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GCGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCCCCAGG ACAGATCATT GATGAACCCA TCCGACCAGT CAACATTCCC 
AGGAAAGAAA AGGATTTCCA AGGGATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTTAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGA 
TTACCGGCAT ATATCCTGTT CATGTGTGTT CGACATGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AACAGCATCA AAAAAGTATT GAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTCTGG CTCTCTAACA CATGCCGATT TTTGCACTGC 
TTGAAACAGT ACAGTGGAGA AGAGGGCTTT ATGAAGCACA ACACATCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CCTGGCTGAG TATCGGCAGG TGCTGAGTGA CTTGGCCATT 
CAGATCTACC AGCAGCTCGT GCGGGTGTTA GAGAACATCC TTCAGCCAAT GATTGTCTCA 
GGCATGCTGG AACATGAAAC GATTCAGGGC GTGTCTGGGG TGAAGCCCAC AGGGTTGAGA 
AAGCGAACCT CCAGTATCGC CGATGAGGGC ACCTACACAC TGGACTCCAT CCTCCGGCAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CATGGCATGG ACCCTGAACT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCATAGGG GCCATCACCC TGAACAACCT TCTCCTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTGGAA 
GAATGGCTGC GTGACAAGAA TCTGATGAAT AGTGGGGCTA AAGAAACCCT GGAACCTCTC 
ATTCAGGCTG CTCAACTTTT GCAAGTGAAA AAGAAAACAG ATGATGATGC AGAAGCCATT 
TGTTCTATGT GCAATGCTTT AACTACTGCC CAGATTGTGA AAGTGTTGAA TTTGTATACT 
CCAGTTAATG AGTTTGAAGA AAGAGTCTCT GTGTCGTTCA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCCCAGCTG CTCATGGATG CTAAACACAT CTTTCCTGTC 
ACCTTTCCTT TCAACCCATC TTCCCTCGCA CTAGAAACCA TCCAGATTCC AGCCAGCCTC 
GGCCTGGGCT TCATTTCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135967.2
CDS	75..5561
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	NM_001142495.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142495.2

ORF Insert Sequence:

ATGGCTGCGT CGGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCAGAGGAA 
GTCTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT CCTCCTGCTT 
CACCTCGAGG AAGGAAAGGA TTTGGAATAC CATCTAGATC CAAAGACCAA GGAGCTGCCT 
CACTTACGAA ATCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCCCT CAGCTATCTT 
CATGAGCCTG CTGTGCTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACGTATTGTG GTATAGTCCT AGTAGCTATA AATCCCTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA CAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATC 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GCGATACTTT 
GCAACTGTGA GTGGTTCTGC CAGTGAGGCC AATGTGGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG CAGCCGTTTT 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATTGGTGC CAATATGAGA 
ACTTATCTTT TAGAGAAATC CAGAGTGGTA TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AGCTTTGTGC CTCAGCAAAG TTACCTGAAT TTAAAATGCT ACGATTAGGA 
AATGCAGATA ACTTTAATTA CACAAAACAA GGAGGCAGTC CTGTGATTGA AGGAGTGGAT 
GATGCAAAGG AGATGGCACA TACTAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAAATGG GAATTTTCCG AATACTTGCT GGCATCCTTC ACTTAGGCAA TGTTGGATTT 
ACATCCCGAG ATGCAGACAG CTGCACAATA CCTCCCAAGC ATGAACCTCT CTGCATCTTC 
TGTGAACTCA TGGGTGTGGA CTATGAGGAG ATGTGTCACT GGCTCTGCCA TCGGAAACTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGC 
GATGCTTTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TAATGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAACAGCAC TCTTTTATTG GTGTGCTAGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTCTTC AAATTGGAGC AAGAAGAATA TATGAAGGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGTATTAA TCTTATAGAA 
TCAAAACTAG GCATTCTAGA TTTACTGGAT GAGGAATGCA AGATGCCTAA AGGCACAGAT 
GACACCTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT CTTTGAAAAG 
CCTCGTCTAT CAAACAAAGC TTTCATCATC CAACATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGAT TTCTCGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCAACTTCAG CCACCTCCTC AGGGCGCACA CCCCTCACAC GAACTCCTGC AAAGCCCACC 
AAAGGCAGAC CAGGCCAAAT GGCCAAAGAG CACAAGAAAA CAGTGGGGCA TCAGTTCAGA 
AACTCCCTGC ACCTGCTTAT GGAGACACTC AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ATGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATC CGAATCAGTG CGGCCGGTTT CCCCTCACGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAC CGTGTCCTAA TGAAGCAGAA AGATGTGCTG 
AGTGACAGAA AGCAAACATG CAAGAATGTG TTAGAGAAAC TGATACTGGA CAAGGACAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCCGGTC AAGTGGCCTA TCTAGAAAAA 
TTGAGAGCTG ACAAACTGAG AGCTGCCTGC ATCCGGATCC AGAAGACCAT CCGAGGGTGG 
CTGCTGAGAA AGAAGTACCT ACGCATGCGG AAGGCAGCCA TCACCATGCA GAGATACGTG 
CGGGGCTACC AGGCCCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCATC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACTATCGTTC TTCAGTCTTA CTTGCGAGGC TTCTTGGCCA GAAATAGGTA TCGCAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGCGAGTCC GGGGCTGGCT GGCCCGCACA 
CACTACAAGA GGAGCATGCA TGCCATCATC TACCTTCAGT GCTGCTTCAG GCGGATGATG 
GCCAAGCGTG AGCTAAAGAA GCTCAAAATC GAGGCTCGCT CAGTGGAGCG CTATAAGAAG 
CTGCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTTGT GGAGAAACTA ACCAATCTGG AAGGAATATA CAACTCTGAG 
ACTGAGAAAC TACGAAGTGA CTTAGAACGT CTTCAACTAA GTGAAGAGGA AGCGAAAGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCCA AGCTCCGGAA AGACCTGGAG 
CAAACTCGTT CAGAGAAAAA ATGCATTGAG GAACATGCAG ATCGATACAA ACAAGAAACA 
GAGCAGCTGG TATCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAAGA AAAAGAAGCC 
CTCAATCACC GCATCGTGCA GCAGGCTAAG GAGATGACAG AAACTATGGA GAAGAAGTTA 
GTAGAAGAAA CGAAACAACT GGAACTCGAC CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAAGAA AGATATGATG ACCTCAAGGA AGAGATGACC 
CTTATGGTGC ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCCACCCA CAGCAGCAAC 
GAGTCTGAAT ATATCTTTAG CTCTGAAATT GCAGAAATGG AAGACATTCC ATCAAGGACA 
GAGGAACCAA GTGAGAAGAA GGTACCTCTG GACATGTCAT TGTTCCTTAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCCAAGGAA GAAGAAAGAC CACAAATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAATCAG AAAACAAAAA ACTGAAGAAT 
GAGCTAAATG AGTTGCGCAA GGCCCTCAGT GAGAAAAGTG CCCCAGAGGT GACCGCCCCA 
GGTGCACCTG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGCGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCTTAAGG TCTCAACTGG TGAGCCAGAA AGAGGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TACTTTTGGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATACATTG GTTTGAAAGA AACAAATAGG 
CTCCTGGAAT CCCAGCTGCA GTCACAGAAG AGGAGCCATG AGAATGAGGC CGAGGCCCTC 
CGTGGGGAGA TCCAGAGCCT GAAGGAGGAG AACAACCGAC AGCAGCAGCT GCTGGCCCAG 
AACCTGCAGC TGCCCCCAGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACCCGG 
CTGACCAACG AAAACTTGGA TTTGATGGAA CAACTTGAAA AACAGGATAA GACGGTCCGT 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GCGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCCCCAGG ACAGATCATT GATGAACCCA TCCGACCAGT CAACATTCCC 
AGGAAAGAAA AGGATTTCCA AGGGATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTTAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGA 
TTACCGGCAT ATATCCTGTT CATGTGTGTT CGACATGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AACAGCATCA AAAAAGTATT GAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTCTGG CTCTCTAACA CATGCCGATT TTTGCACTGC 
TTGAAACAGT ACAGTGGAGA AGAGGGCTTT ATGAAGCACA ACACATCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CCTGGCTGAG TATCGGCAGG TGCTGAGTGA CTTGGCCATT 
CAGATCTACC AGCAGCTCGT GCGGGTGTTA GAGAACATCC TTCAGCCAAT GATTGTCTCA 
GGCATGCTGG AACATGAAAC GATTCAGGGC GTGTCTGGGG TGAAGCCCAC AGGGTTGAGA 
AAGCGAACCT CCAGTATCGC CGATGAGGGC ACCTACACAC TGGACTCCAT CCTCCGGCAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CATGGCATGG ACCCTGAACT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCATAGGG GCCATCACCC TGAACAACCT TCTCCTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTGGAA 
GAATGGCTGC GTGACAAGAA TCTGATGAAT AGTGGGGCTA AAGAAACCCT GGAACCTCTC 
ATTCAGGCTG CTCAACTTTT GCAAGTGAAA AAGAAAACAG ATGATGATGC AGAAGCCATT 
TGTTCTATGT GCAATGCTTT AACTACTGCC CAGATTGTGA AAGTGTTGAA TTTGTATACT 
CCAGTTAATG AGTTTGAAGA AAGAGTCTCT GTGTCGTTCA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCCCAGCTG CTCATGGATG CTAAACACAT CTTTCCTGTC 
ACCTTTCCTT TCAACCCATC TTCCCTCGCA CTAGAAACCA TCCAGATTCC AGCCAGCCTC 
GGCCTGGGCT TCATTTCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521606.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519908.2 Homo sapiens myosin VA (MYO5A), transcript variant X1, mRNA.

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CloneID	OHu85429
Clone ID Related Accession (Same CDS sequence)	XM_011521606.2
Accession Version	XM_011521606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GATCATCTGC TCTGGATTAC CATGAGTTGA ATGAGGATGG AGAGCTGTGG 
CTGGTTTATG AAGGGTTAAA ACAAGCCAAC AGGCTCCTGG AATCCCAGCT GCAGTCACAG 
AAGAGGAGCC ATGAGAATGA GGCCGAGGCC CTCCGTGGGG AGATCCAGAG CCTGAAGGAG 
GAGAACAACC GACAGCAGCA GCTGCTGGCC CAGAACCTGC AGCTGCCCCC AGAGGCCCGC 
ATTGAGGCCA GCCTGCAGCA CGAGATCACC CGGCTGACCA ACGAAAACTT GTATTTTGAG 
GAATTATATG CAGATGACCC TAAGAAGTAT CAATCATATC GGATTTCCCT TTACAAACGG 
ATGATTGATT TGATGGAACA ACTTGAAAAA CAGGATAAGA CGGTCCGTAA ACTGAAAAAA 
CAACTGAAAG TATTTGCCAA AAAAATTGGC GAACTAGAAG TGGGCCAGAT GGAGAACATA 
TCCCCAGGAC AGATCATTGA TGAACCCATC CGACCAGTCA ACATTCCCAG GAAAGAAAAG 
GATTTCCAAG GGATGCTGGA ATACAAGAAG GAGGATGAGC AAAAACTTGT TAAGAACCTG 
ATTCTGGAAC TGAAGCCACG TGGTGTAGCA GTCAATTTGA TTCCAGGATT ACCGGCATAT 
ATCCTGTTCA TGTGTGTTCG ACATGCTGAC TACCTGAATG ATGATCAGAA AGTAAGGTCG 
TTGCTAACAT CAACAATTAA CAGCATCAAA AAAGTATTGA AGAAAAGAGG TGATGATTTT 
GAAACCGTCT CCTTCTGGCT CTCTAACACA TGCCGATTTT TGCACTGCTT GAAACAGTAC 
AGTGGAGAAG AGGGCTTTAT GAAGCACAAC ACATCTCGCC AGAATGAACA CTGCCTCACC 
AATTTTGACC TGGCTGAGTA TCGGCAGGTG CTGAGTGACT TGGCCATTCA GATCTACCAG 
CAGCTCGTGC GGGTGTTAGA GAACATCCTT CAGCCAATGA TTGTCTCAGG CATGCTGGAA 
CATGAAACGA TTCAGGGCGT GTCTGGGGTG AAGCCCACAG GGTTGAGAAA GCGAACCTCC 
AGTATCGCCG ATGAGGGCAC CTACACACTG GACTCCATCC TCCGGCAGCT CAACTCCTTC 
CACTCGGTCA TGTGTCAGCA TGGCATGGAC CCTGAACTGA TCAAGCAGGT GGTCAAGCAG 
ATGTTCTACA TCATAGGGGC CATCACCCTG AACAACCTTC TCCTGCGGAA GGACATGTGC 
TCCTGGAGTA AAGGCATGCA GATCAGGTAC AATGTCAGTC AACTGGAAGA ATGGCTGCGT 
GACAAGAATC TGATGAATAG TGGGGCTAAA GAAACCCTGG AACCTCTCAT TCAGGCTGCT 
CAACTTTTGC AAGTGAAAAA GAAAACAGAT GATGATGCAG AAGCCATTTG TTCTATGTGC 
AATGCTTTAA CTACTGCCCA GATTGTGAAA GTGTTGAATT TGTATACTCC AGTTAATGAG 
TTTGAAGAAA GAGTCTCTGT GTCGTTCATT CGTACTATAC AGATGCGTTT ACGAGACAGG 
AAAGACTCTC CCCAGCTGCT CATGGATGCT AAACACATCT TTCCTGTCAC CTTTCCTTTC 
AACCCATCTT CCCTCGCACT AGAAACCATC CAGATTCCAG CCAGCCTCGG CCTGGGCTTC 
ATTTCACGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519908.2
CDS	8..5722
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521606.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521606.2

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GATCATCTGC TCTGGATTAC CATGAGTTGA ATGAGGATGG AGAGCTGTGG 
CTGGTTTATG AAGGGTTAAA ACAAGCCAAC AGGCTCCTGG AATCCCAGCT GCAGTCACAG 
AAGAGGAGCC ATGAGAATGA GGCCGAGGCC CTCCGTGGGG AGATCCAGAG CCTGAAGGAG 
GAGAACAACC GACAGCAGCA GCTGCTGGCC CAGAACCTGC AGCTGCCCCC AGAGGCCCGC 
ATTGAGGCCA GCCTGCAGCA CGAGATCACC CGGCTGACCA ACGAAAACTT GTATTTTGAG 
GAATTATATG CAGATGACCC TAAGAAGTAT CAATCATATC GGATTTCCCT TTACAAACGG 
ATGATTGATT TGATGGAACA ACTTGAAAAA CAGGATAAGA CGGTCCGTAA ACTGAAAAAA 
CAACTGAAAG TATTTGCCAA AAAAATTGGC GAACTAGAAG TGGGCCAGAT GGAGAACATA 
TCCCCAGGAC AGATCATTGA TGAACCCATC CGACCAGTCA ACATTCCCAG GAAAGAAAAG 
GATTTCCAAG GGATGCTGGA ATACAAGAAG GAGGATGAGC AAAAACTTGT TAAGAACCTG 
ATTCTGGAAC TGAAGCCACG TGGTGTAGCA GTCAATTTGA TTCCAGGATT ACCGGCATAT 
ATCCTGTTCA TGTGTGTTCG ACATGCTGAC TACCTGAATG ATGATCAGAA AGTAAGGTCG 
TTGCTAACAT CAACAATTAA CAGCATCAAA AAAGTATTGA AGAAAAGAGG TGATGATTTT 
GAAACCGTCT CCTTCTGGCT CTCTAACACA TGCCGATTTT TGCACTGCTT GAAACAGTAC 
AGTGGAGAAG AGGGCTTTAT GAAGCACAAC ACATCTCGCC AGAATGAACA CTGCCTCACC 
AATTTTGACC TGGCTGAGTA TCGGCAGGTG CTGAGTGACT TGGCCATTCA GATCTACCAG 
CAGCTCGTGC GGGTGTTAGA GAACATCCTT CAGCCAATGA TTGTCTCAGG CATGCTGGAA 
CATGAAACGA TTCAGGGCGT GTCTGGGGTG AAGCCCACAG GGTTGAGAAA GCGAACCTCC 
AGTATCGCCG ATGAGGGCAC CTACACACTG GACTCCATCC TCCGGCAGCT CAACTCCTTC 
CACTCGGTCA TGTGTCAGCA TGGCATGGAC CCTGAACTGA TCAAGCAGGT GGTCAAGCAG 
ATGTTCTACA TCATAGGGGC CATCACCCTG AACAACCTTC TCCTGCGGAA GGACATGTGC 
TCCTGGAGTA AAGGCATGCA GATCAGGTAC AATGTCAGTC AACTGGAAGA ATGGCTGCGT 
GACAAGAATC TGATGAATAG TGGGGCTAAA GAAACCCTGG AACCTCTCAT TCAGGCTGCT 
CAACTTTTGC AAGTGAAAAA GAAAACAGAT GATGATGCAG AAGCCATTTG TTCTATGTGC 
AATGCTTTAA CTACTGCCCA GATTGTGAAA GTGTTGAATT TGTATACTCC AGTTAATGAG 
TTTGAAGAAA GAGTCTCTGT GTCGTTCATT CGTACTATAC AGATGCGTTT ACGAGACAGG 
AAAGACTCTC CCCAGCTGCT CATGGATGCT AAACACATCT TTCCTGTCAC CTTTCCTTTC 
AACCCATCTT CCCTCGCACT AGAAACCATC CAGATTCCAG CCAGCCTCGG CCTGGGCTTC 
ATTTCACGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521607.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519909.2 Homo sapiens myosin VA (MYO5A), transcript variant X2, mRNA.

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CloneID	OHu85430
Clone ID Related Accession (Same CDS sequence)	XM_011521607.2 , XM_011521607.3
Accession Version	XM_011521607.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521607.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GAATACAATG ACAGATTCCA CAATACTTTT GGAAGATGTA 
CAAAAAATGA AAGATAAAGG TGAAATAGCA CAAGCATACA TTGGTTTGAA AGAAACAAAT 
AGATCATCTG CTCTGGATTA CCATGAGTTG AATGAGGATG GAGAGCTGTG GCTGGTTTAT 
GAAGGGTTAA AACAAGCCAA CAGGCTCCTG GAATCCCAGC TGCAGTCACA GAAGAGGAGC 
CATGAGAATG AGGCCGAGGC CCTCCGTGGG GAGATCCAGA GCCTGAAGGA GGAGAACAAC 
CGACAGCAGC AGCTGCTGGC CCAGAACCTG CAGCTGCCCC CAGAGGCCCG CATTGAGGCC 
AGCCTGCAGC ACGAGATCAC CCGGCTGACC AACGAAAACT TGTATTTTGA GGAATTATAT 
GCAGATGACC CTAAGAAGTA TCAATCATAT CGGATTTCCC TTTACAAACG GATGATTGAT 
TTGATGGAAC AACTTGAAAA ACAGGATAAG ACGGTCCGTA AACTGAAAAA ACAACTGAAA 
GTATTTGCCA AAAAAATTGG CGAACTAGAA GTGGGCCAGA TGGAGAACAT ATCCCCAGGA 
CAGATCATTG ATGAACCCAT CCGACCAGTC AACATTCCCA GGAAAGAAAA GGATTTCCAA 
GGGATGCTGG AATACAAGAA GGAGGATGAG CAAAAACTTG TTAAGAACCT GATTCTGGAA 
CTGAAGCCAC GTGGTGTAGC AGTCAATTTG ATTCCAGGAT TACCGGCATA TATCCTGTTC 
ATGTGTGTTC GACATGCTGA CTACCTGAAT GATGATCAGA AAGTAAGGTC GTTGCTAACA 
TCAACAATTA ACAGCATCAA AAAAGTATTG AAGAAAAGAG GTGATGATTT TGAAACCGTC 
TCCTTCTGGC TCTCTAACAC ATGCCGATTT TTGCACTGCT TGAAACAGTA CAGTGGAGAA 
GAGGGCTTTA TGAAGCACAA CACATCTCGC CAGAATGAAC ACTGCCTCAC CAATTTTGAC 
CTGGCTGAGT ATCGGCAGGT GCTGAGTGAC TTGGCCATTC AGATCTACCA GCAGCTCGTG 
CGGGTGTTAG AGAACATCCT TCAGCCAATG ATTGTCTCAG GCATGCTGGA ACATGAAACG 
ATTCAGGGCG TGTCTGGGGT GAAGCCCACA GGGTTGAGAA AGCGAACCTC CAGTATCGCC 
GATGAGGGCA CCTACACACT GGACTCCATC CTCCGGCAGC TCAACTCCTT CCACTCGGTC 
ATGTGTCAGC ATGGCATGGA CCCTGAACTG ATCAAGCAGG TGGTCAAGCA GATGTTCTAC 
ATCATAGGGG CCATCACCCT GAACAACCTT CTCCTGCGGA AGGACATGTG CTCCTGGAGT 
AAAGGCATGC AGATCAGGTA CAATGTCAGT CAACTGGAAG AATGGCTGCG TGACAAGAAT 
CTGATGAATA GTGGGGCTAA AGAAACCCTG GAACCTCTCA TTCAGGCTGC TCAACTTTTG 
CAAGTGAAAA AGAAAACAGA TGATGATGCA GAAGCCATTT GTTCTATGTG CAATGCTTTA 
ACTACTGCCC AGATTGTGAA AGTGTTGAAT TTGTATACTC CAGTTAATGA GTTTGAAGAA 
AGAGTCTCTG TGTCGTTCAT TCGTACTATA CAGATGCGTT TACGAGACAG GAAAGACTCT 
CCCCAGCTGC TCATGGATGC TAAACACATC TTTCCTGTCA CCTTTCCTTT CAACCCATCT 
TCCCTCGCAC TAGAAACCAT CCAGATTCCA GCCAGCCTCG GCCTGGGCTT CATTTCACGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519909.2
CDS	8..5713
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521607.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521607.2

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GAATACAATG ACAGATTCCA CAATACTTTT GGAAGATGTA 
CAAAAAATGA AAGATAAAGG TGAAATAGCA CAAGCATACA TTGGTTTGAA AGAAACAAAT 
AGATCATCTG CTCTGGATTA CCATGAGTTG AATGAGGATG GAGAGCTGTG GCTGGTTTAT 
GAAGGGTTAA AACAAGCCAA CAGGCTCCTG GAATCCCAGC TGCAGTCACA GAAGAGGAGC 
CATGAGAATG AGGCCGAGGC CCTCCGTGGG GAGATCCAGA GCCTGAAGGA GGAGAACAAC 
CGACAGCAGC AGCTGCTGGC CCAGAACCTG CAGCTGCCCC CAGAGGCCCG CATTGAGGCC 
AGCCTGCAGC ACGAGATCAC CCGGCTGACC AACGAAAACT TGTATTTTGA GGAATTATAT 
GCAGATGACC CTAAGAAGTA TCAATCATAT CGGATTTCCC TTTACAAACG GATGATTGAT 
TTGATGGAAC AACTTGAAAA ACAGGATAAG ACGGTCCGTA AACTGAAAAA ACAACTGAAA 
GTATTTGCCA AAAAAATTGG CGAACTAGAA GTGGGCCAGA TGGAGAACAT ATCCCCAGGA 
CAGATCATTG ATGAACCCAT CCGACCAGTC AACATTCCCA GGAAAGAAAA GGATTTCCAA 
GGGATGCTGG AATACAAGAA GGAGGATGAG CAAAAACTTG TTAAGAACCT GATTCTGGAA 
CTGAAGCCAC GTGGTGTAGC AGTCAATTTG ATTCCAGGAT TACCGGCATA TATCCTGTTC 
ATGTGTGTTC GACATGCTGA CTACCTGAAT GATGATCAGA AAGTAAGGTC GTTGCTAACA 
TCAACAATTA ACAGCATCAA AAAAGTATTG AAGAAAAGAG GTGATGATTT TGAAACCGTC 
TCCTTCTGGC TCTCTAACAC ATGCCGATTT TTGCACTGCT TGAAACAGTA CAGTGGAGAA 
GAGGGCTTTA TGAAGCACAA CACATCTCGC CAGAATGAAC ACTGCCTCAC CAATTTTGAC 
CTGGCTGAGT ATCGGCAGGT GCTGAGTGAC TTGGCCATTC AGATCTACCA GCAGCTCGTG 
CGGGTGTTAG AGAACATCCT TCAGCCAATG ATTGTCTCAG GCATGCTGGA ACATGAAACG 
ATTCAGGGCG TGTCTGGGGT GAAGCCCACA GGGTTGAGAA AGCGAACCTC CAGTATCGCC 
GATGAGGGCA CCTACACACT GGACTCCATC CTCCGGCAGC TCAACTCCTT CCACTCGGTC 
ATGTGTCAGC ATGGCATGGA CCCTGAACTG ATCAAGCAGG TGGTCAAGCA GATGTTCTAC 
ATCATAGGGG CCATCACCCT GAACAACCTT CTCCTGCGGA AGGACATGTG CTCCTGGAGT 
AAAGGCATGC AGATCAGGTA CAATGTCAGT CAACTGGAAG AATGGCTGCG TGACAAGAAT 
CTGATGAATA GTGGGGCTAA AGAAACCCTG GAACCTCTCA TTCAGGCTGC TCAACTTTTG 
CAAGTGAAAA AGAAAACAGA TGATGATGCA GAAGCCATTT GTTCTATGTG CAATGCTTTA 
ACTACTGCCC AGATTGTGAA AGTGTTGAAT TTGTATACTC CAGTTAATGA GTTTGAAGAA 
AGAGTCTCTG TGTCGTTCAT TCGTACTATA CAGATGCGTT TACGAGACAG GAAAGACTCT 
CCCCAGCTGC TCATGGATGC TAAACACATC TTTCCTGTCA CCTTTCCTTT CAACCCATCT 
TCCCTCGCAC TAGAAACCAT CCAGATTCCA GCCAGCCTCG GCCTGGGCTT CATTTCACGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521607.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519909.2 Homo sapiens myosin VA (MYO5A), transcript variant X2, mRNA.

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CloneID	OHu85430
Clone ID Related Accession (Same CDS sequence)	XM_011521607.2 , XM_011521607.3
Accession Version	XM_011521607.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521607.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GAATACAATG ACAGATTCCA CAATACTTTT GGAAGATGTA 
CAAAAAATGA AAGATAAAGG TGAAATAGCA CAAGCATACA TTGGTTTGAA AGAAACAAAT 
AGATCATCTG CTCTGGATTA CCATGAGTTG AATGAGGATG GAGAGCTGTG GCTGGTTTAT 
GAAGGGTTAA AACAAGCCAA CAGGCTCCTG GAATCCCAGC TGCAGTCACA GAAGAGGAGC 
CATGAGAATG AGGCCGAGGC CCTCCGTGGG GAGATCCAGA GCCTGAAGGA GGAGAACAAC 
CGACAGCAGC AGCTGCTGGC CCAGAACCTG CAGCTGCCCC CAGAGGCCCG CATTGAGGCC 
AGCCTGCAGC ACGAGATCAC CCGGCTGACC AACGAAAACT TGTATTTTGA GGAATTATAT 
GCAGATGACC CTAAGAAGTA TCAATCATAT CGGATTTCCC TTTACAAACG GATGATTGAT 
TTGATGGAAC AACTTGAAAA ACAGGATAAG ACGGTCCGTA AACTGAAAAA ACAACTGAAA 
GTATTTGCCA AAAAAATTGG CGAACTAGAA GTGGGCCAGA TGGAGAACAT ATCCCCAGGA 
CAGATCATTG ATGAACCCAT CCGACCAGTC AACATTCCCA GGAAAGAAAA GGATTTCCAA 
GGGATGCTGG AATACAAGAA GGAGGATGAG CAAAAACTTG TTAAGAACCT GATTCTGGAA 
CTGAAGCCAC GTGGTGTAGC AGTCAATTTG ATTCCAGGAT TACCGGCATA TATCCTGTTC 
ATGTGTGTTC GACATGCTGA CTACCTGAAT GATGATCAGA AAGTAAGGTC GTTGCTAACA 
TCAACAATTA ACAGCATCAA AAAAGTATTG AAGAAAAGAG GTGATGATTT TGAAACCGTC 
TCCTTCTGGC TCTCTAACAC ATGCCGATTT TTGCACTGCT TGAAACAGTA CAGTGGAGAA 
GAGGGCTTTA TGAAGCACAA CACATCTCGC CAGAATGAAC ACTGCCTCAC CAATTTTGAC 
CTGGCTGAGT ATCGGCAGGT GCTGAGTGAC TTGGCCATTC AGATCTACCA GCAGCTCGTG 
CGGGTGTTAG AGAACATCCT TCAGCCAATG ATTGTCTCAG GCATGCTGGA ACATGAAACG 
ATTCAGGGCG TGTCTGGGGT GAAGCCCACA GGGTTGAGAA AGCGAACCTC CAGTATCGCC 
GATGAGGGCA CCTACACACT GGACTCCATC CTCCGGCAGC TCAACTCCTT CCACTCGGTC 
ATGTGTCAGC ATGGCATGGA CCCTGAACTG ATCAAGCAGG TGGTCAAGCA GATGTTCTAC 
ATCATAGGGG CCATCACCCT GAACAACCTT CTCCTGCGGA AGGACATGTG CTCCTGGAGT 
AAAGGCATGC AGATCAGGTA CAATGTCAGT CAACTGGAAG AATGGCTGCG TGACAAGAAT 
CTGATGAATA GTGGGGCTAA AGAAACCCTG GAACCTCTCA TTCAGGCTGC TCAACTTTTG 
CAAGTGAAAA AGAAAACAGA TGATGATGCA GAAGCCATTT GTTCTATGTG CAATGCTTTA 
ACTACTGCCC AGATTGTGAA AGTGTTGAAT TTGTATACTC CAGTTAATGA GTTTGAAGAA 
AGAGTCTCTG TGTCGTTCAT TCGTACTATA CAGATGCGTT TACGAGACAG GAAAGACTCT 
CCCCAGCTGC TCATGGATGC TAAACACATC TTTCCTGTCA CCTTTCCTTT CAACCCATCT 
TCCCTCGCAC TAGAAACCAT CCAGATTCCA GCCAGCCTCG GCCTGGGCTT CATTTCACGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519909.2
CDS	8..5713
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521607.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521607.3

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GAATACAATG ACAGATTCCA CAATACTTTT GGAAGATGTA 
CAAAAAATGA AAGATAAAGG TGAAATAGCA CAAGCATACA TTGGTTTGAA AGAAACAAAT 
AGATCATCTG CTCTGGATTA CCATGAGTTG AATGAGGATG GAGAGCTGTG GCTGGTTTAT 
GAAGGGTTAA AACAAGCCAA CAGGCTCCTG GAATCCCAGC TGCAGTCACA GAAGAGGAGC 
CATGAGAATG AGGCCGAGGC CCTCCGTGGG GAGATCCAGA GCCTGAAGGA GGAGAACAAC 
CGACAGCAGC AGCTGCTGGC CCAGAACCTG CAGCTGCCCC CAGAGGCCCG CATTGAGGCC 
AGCCTGCAGC ACGAGATCAC CCGGCTGACC AACGAAAACT TGTATTTTGA GGAATTATAT 
GCAGATGACC CTAAGAAGTA TCAATCATAT CGGATTTCCC TTTACAAACG GATGATTGAT 
TTGATGGAAC AACTTGAAAA ACAGGATAAG ACGGTCCGTA AACTGAAAAA ACAACTGAAA 
GTATTTGCCA AAAAAATTGG CGAACTAGAA GTGGGCCAGA TGGAGAACAT ATCCCCAGGA 
CAGATCATTG ATGAACCCAT CCGACCAGTC AACATTCCCA GGAAAGAAAA GGATTTCCAA 
GGGATGCTGG AATACAAGAA GGAGGATGAG CAAAAACTTG TTAAGAACCT GATTCTGGAA 
CTGAAGCCAC GTGGTGTAGC AGTCAATTTG ATTCCAGGAT TACCGGCATA TATCCTGTTC 
ATGTGTGTTC GACATGCTGA CTACCTGAAT GATGATCAGA AAGTAAGGTC GTTGCTAACA 
TCAACAATTA ACAGCATCAA AAAAGTATTG AAGAAAAGAG GTGATGATTT TGAAACCGTC 
TCCTTCTGGC TCTCTAACAC ATGCCGATTT TTGCACTGCT TGAAACAGTA CAGTGGAGAA 
GAGGGCTTTA TGAAGCACAA CACATCTCGC CAGAATGAAC ACTGCCTCAC CAATTTTGAC 
CTGGCTGAGT ATCGGCAGGT GCTGAGTGAC TTGGCCATTC AGATCTACCA GCAGCTCGTG 
CGGGTGTTAG AGAACATCCT TCAGCCAATG ATTGTCTCAG GCATGCTGGA ACATGAAACG 
ATTCAGGGCG TGTCTGGGGT GAAGCCCACA GGGTTGAGAA AGCGAACCTC CAGTATCGCC 
GATGAGGGCA CCTACACACT GGACTCCATC CTCCGGCAGC TCAACTCCTT CCACTCGGTC 
ATGTGTCAGC ATGGCATGGA CCCTGAACTG ATCAAGCAGG TGGTCAAGCA GATGTTCTAC 
ATCATAGGGG CCATCACCCT GAACAACCTT CTCCTGCGGA AGGACATGTG CTCCTGGAGT 
AAAGGCATGC AGATCAGGTA CAATGTCAGT CAACTGGAAG AATGGCTGCG TGACAAGAAT 
CTGATGAATA GTGGGGCTAA AGAAACCCTG GAACCTCTCA TTCAGGCTGC TCAACTTTTG 
CAAGTGAAAA AGAAAACAGA TGATGATGCA GAAGCCATTT GTTCTATGTG CAATGCTTTA 
ACTACTGCCC AGATTGTGAA AGTGTTGAAT TTGTATACTC CAGTTAATGA GTTTGAAGAA 
AGAGTCTCTG TGTCGTTCAT TCGTACTATA CAGATGCGTT TACGAGACAG GAAAGACTCT 
CCCCAGCTGC TCATGGATGC TAAACACATC TTTCCTGTCA CCTTTCCTTT CAACCCATCT 
TCCCTCGCAC TAGAAACCAT CCAGATTCCA GCCAGCCTCG GCCTGGGCTT CATTTCACGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521608.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519910.2 Homo sapiens myosin VA (MYO5A), transcript variant X4, mRNA.

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CloneID	OHu85431
Clone ID Related Accession (Same CDS sequence)	XM_011521608.3 , XM_011521608.2
Accession Version	XM_011521608.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521608.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GATCATCTGC TCTGGATTAC CATGAGTTGA ATGAGGATGG AGAGCTGTGG 
CTGGTTTATG AAGGGTTAAA ACAAGCCAAC AGGCTCCTGG AATCCCAGCT GCAGTCACAG 
AAGAGGAGCC ATGAGAATGA GGCCGAGGCC CTCCGTGGGG AGATCCAGAG CCTGAAGGAG 
GAGAACAACC GACAGCAGCA GCTGCTGGCC CAGAACCTGC AGCTGCCCCC AGAGGCCCGC 
ATTGAGGCCA GCCTGCAGCA CGAGATCACC CGGCTGACCA ACGAAAACTT GGATTTGATG 
GAACAACTTG AAAAACAGGA TAAGACGGTC CGTAAACTGA AAAAACAACT GAAAGTATTT 
GCCAAAAAAA TTGGCGAACT AGAAGTGGGC CAGATGGAGA ACATATCCCC AGGACAGATC 
ATTGATGAAC CCATCCGACC AGTCAACATT CCCAGGAAAG AAAAGGATTT CCAAGGGATG 
CTGGAATACA AGAAGGAGGA TGAGCAAAAA CTTGTTAAGA ACCTGATTCT GGAACTGAAG 
CCACGTGGTG TAGCAGTCAA TTTGATTCCA GGATTACCGG CATATATCCT GTTCATGTGT 
GTTCGACATG CTGACTACCT GAATGATGAT CAGAAAGTAA GGTCGTTGCT AACATCAACA 
ATTAACAGCA TCAAAAAAGT ATTGAAGAAA AGAGGTGATG ATTTTGAAAC CGTCTCCTTC 
TGGCTCTCTA ACACATGCCG ATTTTTGCAC TGCTTGAAAC AGTACAGTGG AGAAGAGGGC 
TTTATGAAGC ACAACACATC TCGCCAGAAT GAACACTGCC TCACCAATTT TGACCTGGCT 
GAGTATCGGC AGGTGCTGAG TGACTTGGCC ATTCAGATCT ACCAGCAGCT CGTGCGGGTG 
TTAGAGAACA TCCTTCAGCC AATGATTGTC TCAGGCATGC TGGAACATGA AACGATTCAG 
GGCGTGTCTG GGGTGAAGCC CACAGGGTTG AGAAAGCGAA CCTCCAGTAT CGCCGATGAG 
GGCACCTACA CACTGGACTC CATCCTCCGG CAGCTCAACT CCTTCCACTC GGTCATGTGT 
CAGCATGGCA TGGACCCTGA ACTGATCAAG CAGGTGGTCA AGCAGATGTT CTACATCATA 
GGGGCCATCA CCCTGAACAA CCTTCTCCTG CGGAAGGACA TGTGCTCCTG GAGTAAAGGC 
ATGCAGATCA GGTACAATGT CAGTCAACTG GAAGAATGGC TGCGTGACAA GAATCTGATG 
AATAGTGGGG CTAAAGAAAC CCTGGAACCT CTCATTCAGG CTGCTCAACT TTTGCAAGTG 
AAAAAGAAAA CAGATGATGA TGCAGAAGCC ATTTGTTCTA TGTGCAATGC TTTAACTACT 
GCCCAGATTG TGAAAGTGTT GAATTTGTAT ACTCCAGTTA ATGAGTTTGA AGAAAGAGTC 
TCTGTGTCGT TCATTCGTAC TATACAGATG CGTTTACGAG ACAGGAAAGA CTCTCCCCAG 
CTGCTCATGG ATGCTAAACA CATCTTTCCT GTCACCTTTC CTTTCAACCC ATCTTCCCTC 
GCACTAGAAA CCATCCAGAT TCCAGCCAGC CTCGGCCTGG GCTTCATTTC ACGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519910.2
CDS	7..5646
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521608.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521608.3

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GATCATCTGC TCTGGATTAC CATGAGTTGA ATGAGGATGG AGAGCTGTGG 
CTGGTTTATG AAGGGTTAAA ACAAGCCAAC AGGCTCCTGG AATCCCAGCT GCAGTCACAG 
AAGAGGAGCC ATGAGAATGA GGCCGAGGCC CTCCGTGGGG AGATCCAGAG CCTGAAGGAG 
GAGAACAACC GACAGCAGCA GCTGCTGGCC CAGAACCTGC AGCTGCCCCC AGAGGCCCGC 
ATTGAGGCCA GCCTGCAGCA CGAGATCACC CGGCTGACCA ACGAAAACTT GGATTTGATG 
GAACAACTTG AAAAACAGGA TAAGACGGTC CGTAAACTGA AAAAACAACT GAAAGTATTT 
GCCAAAAAAA TTGGCGAACT AGAAGTGGGC CAGATGGAGA ACATATCCCC AGGACAGATC 
ATTGATGAAC CCATCCGACC AGTCAACATT CCCAGGAAAG AAAAGGATTT CCAAGGGATG 
CTGGAATACA AGAAGGAGGA TGAGCAAAAA CTTGTTAAGA ACCTGATTCT GGAACTGAAG 
CCACGTGGTG TAGCAGTCAA TTTGATTCCA GGATTACCGG CATATATCCT GTTCATGTGT 
GTTCGACATG CTGACTACCT GAATGATGAT CAGAAAGTAA GGTCGTTGCT AACATCAACA 
ATTAACAGCA TCAAAAAAGT ATTGAAGAAA AGAGGTGATG ATTTTGAAAC CGTCTCCTTC 
TGGCTCTCTA ACACATGCCG ATTTTTGCAC TGCTTGAAAC AGTACAGTGG AGAAGAGGGC 
TTTATGAAGC ACAACACATC TCGCCAGAAT GAACACTGCC TCACCAATTT TGACCTGGCT 
GAGTATCGGC AGGTGCTGAG TGACTTGGCC ATTCAGATCT ACCAGCAGCT CGTGCGGGTG 
TTAGAGAACA TCCTTCAGCC AATGATTGTC TCAGGCATGC TGGAACATGA AACGATTCAG 
GGCGTGTCTG GGGTGAAGCC CACAGGGTTG AGAAAGCGAA CCTCCAGTAT CGCCGATGAG 
GGCACCTACA CACTGGACTC CATCCTCCGG CAGCTCAACT CCTTCCACTC GGTCATGTGT 
CAGCATGGCA TGGACCCTGA ACTGATCAAG CAGGTGGTCA AGCAGATGTT CTACATCATA 
GGGGCCATCA CCCTGAACAA CCTTCTCCTG CGGAAGGACA TGTGCTCCTG GAGTAAAGGC 
ATGCAGATCA GGTACAATGT CAGTCAACTG GAAGAATGGC TGCGTGACAA GAATCTGATG 
AATAGTGGGG CTAAAGAAAC CCTGGAACCT CTCATTCAGG CTGCTCAACT TTTGCAAGTG 
AAAAAGAAAA CAGATGATGA TGCAGAAGCC ATTTGTTCTA TGTGCAATGC TTTAACTACT 
GCCCAGATTG TGAAAGTGTT GAATTTGTAT ACTCCAGTTA ATGAGTTTGA AGAAAGAGTC 
TCTGTGTCGT TCATTCGTAC TATACAGATG CGTTTACGAG ACAGGAAAGA CTCTCCCCAG 
CTGCTCATGG ATGCTAAACA CATCTTTCCT GTCACCTTTC CTTTCAACCC ATCTTCCCTC 
GCACTAGAAA CCATCCAGAT TCCAGCCAGC CTCGGCCTGG GCTTCATTTC ACGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521608.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519910.2 Homo sapiens myosin VA (MYO5A), transcript variant X4, mRNA.

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CloneID	OHu85431
Clone ID Related Accession (Same CDS sequence)	XM_011521608.3 , XM_011521608.2
Accession Version	XM_011521608.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521608.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GATCATCTGC TCTGGATTAC CATGAGTTGA ATGAGGATGG AGAGCTGTGG 
CTGGTTTATG AAGGGTTAAA ACAAGCCAAC AGGCTCCTGG AATCCCAGCT GCAGTCACAG 
AAGAGGAGCC ATGAGAATGA GGCCGAGGCC CTCCGTGGGG AGATCCAGAG CCTGAAGGAG 
GAGAACAACC GACAGCAGCA GCTGCTGGCC CAGAACCTGC AGCTGCCCCC AGAGGCCCGC 
ATTGAGGCCA GCCTGCAGCA CGAGATCACC CGGCTGACCA ACGAAAACTT GGATTTGATG 
GAACAACTTG AAAAACAGGA TAAGACGGTC CGTAAACTGA AAAAACAACT GAAAGTATTT 
GCCAAAAAAA TTGGCGAACT AGAAGTGGGC CAGATGGAGA ACATATCCCC AGGACAGATC 
ATTGATGAAC CCATCCGACC AGTCAACATT CCCAGGAAAG AAAAGGATTT CCAAGGGATG 
CTGGAATACA AGAAGGAGGA TGAGCAAAAA CTTGTTAAGA ACCTGATTCT GGAACTGAAG 
CCACGTGGTG TAGCAGTCAA TTTGATTCCA GGATTACCGG CATATATCCT GTTCATGTGT 
GTTCGACATG CTGACTACCT GAATGATGAT CAGAAAGTAA GGTCGTTGCT AACATCAACA 
ATTAACAGCA TCAAAAAAGT ATTGAAGAAA AGAGGTGATG ATTTTGAAAC CGTCTCCTTC 
TGGCTCTCTA ACACATGCCG ATTTTTGCAC TGCTTGAAAC AGTACAGTGG AGAAGAGGGC 
TTTATGAAGC ACAACACATC TCGCCAGAAT GAACACTGCC TCACCAATTT TGACCTGGCT 
GAGTATCGGC AGGTGCTGAG TGACTTGGCC ATTCAGATCT ACCAGCAGCT CGTGCGGGTG 
TTAGAGAACA TCCTTCAGCC AATGATTGTC TCAGGCATGC TGGAACATGA AACGATTCAG 
GGCGTGTCTG GGGTGAAGCC CACAGGGTTG AGAAAGCGAA CCTCCAGTAT CGCCGATGAG 
GGCACCTACA CACTGGACTC CATCCTCCGG CAGCTCAACT CCTTCCACTC GGTCATGTGT 
CAGCATGGCA TGGACCCTGA ACTGATCAAG CAGGTGGTCA AGCAGATGTT CTACATCATA 
GGGGCCATCA CCCTGAACAA CCTTCTCCTG CGGAAGGACA TGTGCTCCTG GAGTAAAGGC 
ATGCAGATCA GGTACAATGT CAGTCAACTG GAAGAATGGC TGCGTGACAA GAATCTGATG 
AATAGTGGGG CTAAAGAAAC CCTGGAACCT CTCATTCAGG CTGCTCAACT TTTGCAAGTG 
AAAAAGAAAA CAGATGATGA TGCAGAAGCC ATTTGTTCTA TGTGCAATGC TTTAACTACT 
GCCCAGATTG TGAAAGTGTT GAATTTGTAT ACTCCAGTTA ATGAGTTTGA AGAAAGAGTC 
TCTGTGTCGT TCATTCGTAC TATACAGATG CGTTTACGAG ACAGGAAAGA CTCTCCCCAG 
CTGCTCATGG ATGCTAAACA CATCTTTCCT GTCACCTTTC CTTTCAACCC ATCTTCCCTC 
GCACTAGAAA CCATCCAGAT TCCAGCCAGC CTCGGCCTGG GCTTCATTTC ACGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519910.2
CDS	7..5646
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521608.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521608.2

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GATCATCTGC TCTGGATTAC CATGAGTTGA ATGAGGATGG AGAGCTGTGG 
CTGGTTTATG AAGGGTTAAA ACAAGCCAAC AGGCTCCTGG AATCCCAGCT GCAGTCACAG 
AAGAGGAGCC ATGAGAATGA GGCCGAGGCC CTCCGTGGGG AGATCCAGAG CCTGAAGGAG 
GAGAACAACC GACAGCAGCA GCTGCTGGCC CAGAACCTGC AGCTGCCCCC AGAGGCCCGC 
ATTGAGGCCA GCCTGCAGCA CGAGATCACC CGGCTGACCA ACGAAAACTT GGATTTGATG 
GAACAACTTG AAAAACAGGA TAAGACGGTC CGTAAACTGA AAAAACAACT GAAAGTATTT 
GCCAAAAAAA TTGGCGAACT AGAAGTGGGC CAGATGGAGA ACATATCCCC AGGACAGATC 
ATTGATGAAC CCATCCGACC AGTCAACATT CCCAGGAAAG AAAAGGATTT CCAAGGGATG 
CTGGAATACA AGAAGGAGGA TGAGCAAAAA CTTGTTAAGA ACCTGATTCT GGAACTGAAG 
CCACGTGGTG TAGCAGTCAA TTTGATTCCA GGATTACCGG CATATATCCT GTTCATGTGT 
GTTCGACATG CTGACTACCT GAATGATGAT CAGAAAGTAA GGTCGTTGCT AACATCAACA 
ATTAACAGCA TCAAAAAAGT ATTGAAGAAA AGAGGTGATG ATTTTGAAAC CGTCTCCTTC 
TGGCTCTCTA ACACATGCCG ATTTTTGCAC TGCTTGAAAC AGTACAGTGG AGAAGAGGGC 
TTTATGAAGC ACAACACATC TCGCCAGAAT GAACACTGCC TCACCAATTT TGACCTGGCT 
GAGTATCGGC AGGTGCTGAG TGACTTGGCC ATTCAGATCT ACCAGCAGCT CGTGCGGGTG 
TTAGAGAACA TCCTTCAGCC AATGATTGTC TCAGGCATGC TGGAACATGA AACGATTCAG 
GGCGTGTCTG GGGTGAAGCC CACAGGGTTG AGAAAGCGAA CCTCCAGTAT CGCCGATGAG 
GGCACCTACA CACTGGACTC CATCCTCCGG CAGCTCAACT CCTTCCACTC GGTCATGTGT 
CAGCATGGCA TGGACCCTGA ACTGATCAAG CAGGTGGTCA AGCAGATGTT CTACATCATA 
GGGGCCATCA CCCTGAACAA CCTTCTCCTG CGGAAGGACA TGTGCTCCTG GAGTAAAGGC 
ATGCAGATCA GGTACAATGT CAGTCAACTG GAAGAATGGC TGCGTGACAA GAATCTGATG 
AATAGTGGGG CTAAAGAAAC CCTGGAACCT CTCATTCAGG CTGCTCAACT TTTGCAAGTG 
AAAAAGAAAA CAGATGATGA TGCAGAAGCC ATTTGTTCTA TGTGCAATGC TTTAACTACT 
GCCCAGATTG TGAAAGTGTT GAATTTGTAT ACTCCAGTTA ATGAGTTTGA AGAAAGAGTC 
TCTGTGTCGT TCATTCGTAC TATACAGATG CGTTTACGAG ACAGGAAAGA CTCTCCCCAG 
CTGCTCATGG ATGCTAAACA CATCTTTCCT GTCACCTTTC CTTTCAACCC ATCTTCCCTC 
GCACTAGAAA CCATCCAGAT TCCAGCCAGC CTCGGCCTGG GCTTCATTTC ACGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521609.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519911.2 Homo sapiens myosin VA (MYO5A), transcript variant X5, mRNA.

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CloneID	OHu85432
Clone ID Related Accession (Same CDS sequence)	XM_011521609.3 , XM_011521609.2
Accession Version	XM_011521609.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521609.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GGCTCCTGGA ATCCCAGCTG CAGTCACAGA AGAGGAGCCA TGAGAATGAG 
GCCGAGGCCC TCCGTGGGGA GATCCAGAGC CTGAAGGAGG AGAACAACCG ACAGCAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACCC GGCTGACCAA CGAAAACTTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCCCTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTTGAAAAAC AGGATAAGAC GGTCCGTAAA CTGAAAAAAC AACTGAAAGT ATTTGCCAAA 
AAAATTGGCG AACTAGAAGT GGGCCAGATG GAGAACATAT CCCCAGGACA GATCATTGAT 
GAACCCATCC GACCAGTCAA CATTCCCAGG AAAGAAAAGG ATTTCCAAGG GATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTT AAGAACCTGA TTCTGGAACT GAAGCCACGT 
GGTGTAGCAG TCAATTTGAT TCCAGGATTA CCGGCATATA TCCTGTTCAT GTGTGTTCGA 
CATGCTGACT ACCTGAATGA TGATCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAC 
AGCATCAAAA AAGTATTGAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTCTGGCTC 
TCTAACACAT GCCGATTTTT GCACTGCTTG AAACAGTACA GTGGAGAAGA GGGCTTTATG 
AAGCACAACA CATCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACCT GGCTGAGTAT 
CGGCAGGTGC TGAGTGACTT GGCCATTCAG ATCTACCAGC AGCTCGTGCG GGTGTTAGAG 
AACATCCTTC AGCCAATGAT TGTCTCAGGC ATGCTGGAAC ATGAAACGAT TCAGGGCGTG 
TCTGGGGTGA AGCCCACAGG GTTGAGAAAG CGAACCTCCA GTATCGCCGA TGAGGGCACC 
TACACACTGG ACTCCATCCT CCGGCAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAT 
GGCATGGACC CTGAACTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT CATAGGGGCC 
ATCACCCTGA ACAACCTTCT CCTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTGGAAGAA TGGCTGCGTG ACAAGAATCT GATGAATAGT 
GGGGCTAAAG AAACCCTGGA ACCTCTCATT CAGGCTGCTC AACTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGATGCAGA AGCCATTTGT TCTATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTGAAAG TGTTGAATTT GTATACTCCA GTTAATGAGT TTGAAGAAAG AGTCTCTGTG 
TCGTTCATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC CCAGCTGCTC 
ATGGATGCTA AACACATCTT TCCTGTCACC TTTCCTTTCA ACCCATCTTC CCTCGCACTA 
GAAACCATCC AGATTCCAGC CAGCCTCGGC CTGGGCTTCA TTTCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519911.2
CDS	7..5640
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521609.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521609.3

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GGCTCCTGGA ATCCCAGCTG CAGTCACAGA AGAGGAGCCA TGAGAATGAG 
GCCGAGGCCC TCCGTGGGGA GATCCAGAGC CTGAAGGAGG AGAACAACCG ACAGCAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACCC GGCTGACCAA CGAAAACTTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCCCTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTTGAAAAAC AGGATAAGAC GGTCCGTAAA CTGAAAAAAC AACTGAAAGT ATTTGCCAAA 
AAAATTGGCG AACTAGAAGT GGGCCAGATG GAGAACATAT CCCCAGGACA GATCATTGAT 
GAACCCATCC GACCAGTCAA CATTCCCAGG AAAGAAAAGG ATTTCCAAGG GATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTT AAGAACCTGA TTCTGGAACT GAAGCCACGT 
GGTGTAGCAG TCAATTTGAT TCCAGGATTA CCGGCATATA TCCTGTTCAT GTGTGTTCGA 
CATGCTGACT ACCTGAATGA TGATCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAC 
AGCATCAAAA AAGTATTGAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTCTGGCTC 
TCTAACACAT GCCGATTTTT GCACTGCTTG AAACAGTACA GTGGAGAAGA GGGCTTTATG 
AAGCACAACA CATCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACCT GGCTGAGTAT 
CGGCAGGTGC TGAGTGACTT GGCCATTCAG ATCTACCAGC AGCTCGTGCG GGTGTTAGAG 
AACATCCTTC AGCCAATGAT TGTCTCAGGC ATGCTGGAAC ATGAAACGAT TCAGGGCGTG 
TCTGGGGTGA AGCCCACAGG GTTGAGAAAG CGAACCTCCA GTATCGCCGA TGAGGGCACC 
TACACACTGG ACTCCATCCT CCGGCAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAT 
GGCATGGACC CTGAACTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT CATAGGGGCC 
ATCACCCTGA ACAACCTTCT CCTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTGGAAGAA TGGCTGCGTG ACAAGAATCT GATGAATAGT 
GGGGCTAAAG AAACCCTGGA ACCTCTCATT CAGGCTGCTC AACTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGATGCAGA AGCCATTTGT TCTATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTGAAAG TGTTGAATTT GTATACTCCA GTTAATGAGT TTGAAGAAAG AGTCTCTGTG 
TCGTTCATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC CCAGCTGCTC 
ATGGATGCTA AACACATCTT TCCTGTCACC TTTCCTTTCA ACCCATCTTC CCTCGCACTA 
GAAACCATCC AGATTCCAGC CAGCCTCGGC CTGGGCTTCA TTTCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521609.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519911.2 Homo sapiens myosin VA (MYO5A), transcript variant X5, mRNA.

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CloneID	OHu85432
Clone ID Related Accession (Same CDS sequence)	XM_011521609.3 , XM_011521609.2
Accession Version	XM_011521609.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521609.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GGCTCCTGGA ATCCCAGCTG CAGTCACAGA AGAGGAGCCA TGAGAATGAG 
GCCGAGGCCC TCCGTGGGGA GATCCAGAGC CTGAAGGAGG AGAACAACCG ACAGCAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACCC GGCTGACCAA CGAAAACTTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCCCTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTTGAAAAAC AGGATAAGAC GGTCCGTAAA CTGAAAAAAC AACTGAAAGT ATTTGCCAAA 
AAAATTGGCG AACTAGAAGT GGGCCAGATG GAGAACATAT CCCCAGGACA GATCATTGAT 
GAACCCATCC GACCAGTCAA CATTCCCAGG AAAGAAAAGG ATTTCCAAGG GATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTT AAGAACCTGA TTCTGGAACT GAAGCCACGT 
GGTGTAGCAG TCAATTTGAT TCCAGGATTA CCGGCATATA TCCTGTTCAT GTGTGTTCGA 
CATGCTGACT ACCTGAATGA TGATCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAC 
AGCATCAAAA AAGTATTGAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTCTGGCTC 
TCTAACACAT GCCGATTTTT GCACTGCTTG AAACAGTACA GTGGAGAAGA GGGCTTTATG 
AAGCACAACA CATCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACCT GGCTGAGTAT 
CGGCAGGTGC TGAGTGACTT GGCCATTCAG ATCTACCAGC AGCTCGTGCG GGTGTTAGAG 
AACATCCTTC AGCCAATGAT TGTCTCAGGC ATGCTGGAAC ATGAAACGAT TCAGGGCGTG 
TCTGGGGTGA AGCCCACAGG GTTGAGAAAG CGAACCTCCA GTATCGCCGA TGAGGGCACC 
TACACACTGG ACTCCATCCT CCGGCAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAT 
GGCATGGACC CTGAACTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT CATAGGGGCC 
ATCACCCTGA ACAACCTTCT CCTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTGGAAGAA TGGCTGCGTG ACAAGAATCT GATGAATAGT 
GGGGCTAAAG AAACCCTGGA ACCTCTCATT CAGGCTGCTC AACTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGATGCAGA AGCCATTTGT TCTATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTGAAAG TGTTGAATTT GTATACTCCA GTTAATGAGT TTGAAGAAAG AGTCTCTGTG 
TCGTTCATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC CCAGCTGCTC 
ATGGATGCTA AACACATCTT TCCTGTCACC TTTCCTTTCA ACCCATCTTC CCTCGCACTA 
GAAACCATCC AGATTCCAGC CAGCCTCGGC CTGGGCTTCA TTTCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519911.2
CDS	7..5640
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521609.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521609.2

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GGCTCCTGGA ATCCCAGCTG CAGTCACAGA AGAGGAGCCA TGAGAATGAG 
GCCGAGGCCC TCCGTGGGGA GATCCAGAGC CTGAAGGAGG AGAACAACCG ACAGCAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACCC GGCTGACCAA CGAAAACTTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCCCTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTTGAAAAAC AGGATAAGAC GGTCCGTAAA CTGAAAAAAC AACTGAAAGT ATTTGCCAAA 
AAAATTGGCG AACTAGAAGT GGGCCAGATG GAGAACATAT CCCCAGGACA GATCATTGAT 
GAACCCATCC GACCAGTCAA CATTCCCAGG AAAGAAAAGG ATTTCCAAGG GATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTT AAGAACCTGA TTCTGGAACT GAAGCCACGT 
GGTGTAGCAG TCAATTTGAT TCCAGGATTA CCGGCATATA TCCTGTTCAT GTGTGTTCGA 
CATGCTGACT ACCTGAATGA TGATCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAC 
AGCATCAAAA AAGTATTGAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTCTGGCTC 
TCTAACACAT GCCGATTTTT GCACTGCTTG AAACAGTACA GTGGAGAAGA GGGCTTTATG 
AAGCACAACA CATCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACCT GGCTGAGTAT 
CGGCAGGTGC TGAGTGACTT GGCCATTCAG ATCTACCAGC AGCTCGTGCG GGTGTTAGAG 
AACATCCTTC AGCCAATGAT TGTCTCAGGC ATGCTGGAAC ATGAAACGAT TCAGGGCGTG 
TCTGGGGTGA AGCCCACAGG GTTGAGAAAG CGAACCTCCA GTATCGCCGA TGAGGGCACC 
TACACACTGG ACTCCATCCT CCGGCAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAT 
GGCATGGACC CTGAACTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT CATAGGGGCC 
ATCACCCTGA ACAACCTTCT CCTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTGGAAGAA TGGCTGCGTG ACAAGAATCT GATGAATAGT 
GGGGCTAAAG AAACCCTGGA ACCTCTCATT CAGGCTGCTC AACTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGATGCAGA AGCCATTTGT TCTATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTGAAAG TGTTGAATTT GTATACTCCA GTTAATGAGT TTGAAGAAAG AGTCTCTGTG 
TCGTTCATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC CCAGCTGCTC 
ATGGATGCTA AACACATCTT TCCTGTCACC TTTCCTTTCA ACCCATCTTC CCTCGCACTA 
GAAACCATCC AGATTCCAGC CAGCCTCGGC CTGGGCTTCA TTTCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521610.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519912.2 Homo sapiens myosin VA (MYO5A), transcript variant X6, mRNA.

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CloneID	OHu85433
Clone ID Related Accession (Same CDS sequence)	XM_011521610.2 , XM_011521610.3
Accession Version	XM_011521610.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521610.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GAATACAATG ACAGATTCCA CAATACTTTT GGAAGATGTA 
CAAAAAATGA AAGATAAAGG TGAAATAGCA CAAGCATACA TTGGTTTGAA AGAAACAAAT 
AGATCATCTG CTCTGGATTA CCATGAGTTG AATGAGGATG GAGAGCTGTG GCTGGTTTAT 
GAAGGGTTAA AACAAGCCAA CAGGCTCCTG GAATCCCAGC TGCAGTCACA GAAGAGGAGC 
CATGAGAATG AGGCCGAGGC CCTCCGTGGG GAGATCCAGA GCCTGAAGGA GGAGAACAAC 
CGACAGCAGC AGCTGCTGGC CCAGAACCTG CAGCTGCCCC CAGAGGCCCG CATTGAGGCC 
AGCCTGCAGC ACGAGATCAC CCGGCTGACC AACGAAAACT TGGATTTGAT GGAACAACTT 
GAAAAACAGG ATAAGACGGT CCGTAAACTG AAAAAACAAC TGAAAGTATT TGCCAAAAAA 
ATTGGCGAAC TAGAAGTGGG CCAGATGGAG AACATATCCC CAGGACAGAT CATTGATGAA 
CCCATCCGAC CAGTCAACAT TCCCAGGAAA GAAAAGGATT TCCAAGGGAT GCTGGAATAC 
AAGAAGGAGG ATGAGCAAAA ACTTGTTAAG AACCTGATTC TGGAACTGAA GCCACGTGGT 
GTAGCAGTCA ATTTGATTCC AGGATTACCG GCATATATCC TGTTCATGTG TGTTCGACAT 
GCTGACTACC TGAATGATGA TCAGAAAGTA AGGTCGTTGC TAACATCAAC AATTAACAGC 
ATCAAAAAAG TATTGAAGAA AAGAGGTGAT GATTTTGAAA CCGTCTCCTT CTGGCTCTCT 
AACACATGCC GATTTTTGCA CTGCTTGAAA CAGTACAGTG GAGAAGAGGG CTTTATGAAG 
CACAACACAT CTCGCCAGAA TGAACACTGC CTCACCAATT TTGACCTGGC TGAGTATCGG 
CAGGTGCTGA GTGACTTGGC CATTCAGATC TACCAGCAGC TCGTGCGGGT GTTAGAGAAC 
ATCCTTCAGC CAATGATTGT CTCAGGCATG CTGGAACATG AAACGATTCA GGGCGTGTCT 
GGGGTGAAGC CCACAGGGTT GAGAAAGCGA ACCTCCAGTA TCGCCGATGA GGGCACCTAC 
ACACTGGACT CCATCCTCCG GCAGCTCAAC TCCTTCCACT CGGTCATGTG TCAGCATGGC 
ATGGACCCTG AACTGATCAA GCAGGTGGTC AAGCAGATGT TCTACATCAT AGGGGCCATC 
ACCCTGAACA ACCTTCTCCT GCGGAAGGAC ATGTGCTCCT GGAGTAAAGG CATGCAGATC 
AGGTACAATG TCAGTCAACT GGAAGAATGG CTGCGTGACA AGAATCTGAT GAATAGTGGG 
GCTAAAGAAA CCCTGGAACC TCTCATTCAG GCTGCTCAAC TTTTGCAAGT GAAAAAGAAA 
ACAGATGATG ATGCAGAAGC CATTTGTTCT ATGTGCAATG CTTTAACTAC TGCCCAGATT 
GTGAAAGTGT TGAATTTGTA TACTCCAGTT AATGAGTTTG AAGAAAGAGT CTCTGTGTCG 
TTCATTCGTA CTATACAGAT GCGTTTACGA GACAGGAAAG ACTCTCCCCA GCTGCTCATG 
GATGCTAAAC ACATCTTTCC TGTCACCTTT CCTTTCAACC CATCTTCCCT CGCACTAGAA 
ACCATCCAGA TTCCAGCCAG CCTCGGCCTG GGCTTCATTT CACGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519912.2
CDS	8..5638
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521610.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521610.2

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GAATACAATG ACAGATTCCA CAATACTTTT GGAAGATGTA 
CAAAAAATGA AAGATAAAGG TGAAATAGCA CAAGCATACA TTGGTTTGAA AGAAACAAAT 
AGATCATCTG CTCTGGATTA CCATGAGTTG AATGAGGATG GAGAGCTGTG GCTGGTTTAT 
GAAGGGTTAA AACAAGCCAA CAGGCTCCTG GAATCCCAGC TGCAGTCACA GAAGAGGAGC 
CATGAGAATG AGGCCGAGGC CCTCCGTGGG GAGATCCAGA GCCTGAAGGA GGAGAACAAC 
CGACAGCAGC AGCTGCTGGC CCAGAACCTG CAGCTGCCCC CAGAGGCCCG CATTGAGGCC 
AGCCTGCAGC ACGAGATCAC CCGGCTGACC AACGAAAACT TGGATTTGAT GGAACAACTT 
GAAAAACAGG ATAAGACGGT CCGTAAACTG AAAAAACAAC TGAAAGTATT TGCCAAAAAA 
ATTGGCGAAC TAGAAGTGGG CCAGATGGAG AACATATCCC CAGGACAGAT CATTGATGAA 
CCCATCCGAC CAGTCAACAT TCCCAGGAAA GAAAAGGATT TCCAAGGGAT GCTGGAATAC 
AAGAAGGAGG ATGAGCAAAA ACTTGTTAAG AACCTGATTC TGGAACTGAA GCCACGTGGT 
GTAGCAGTCA ATTTGATTCC AGGATTACCG GCATATATCC TGTTCATGTG TGTTCGACAT 
GCTGACTACC TGAATGATGA TCAGAAAGTA AGGTCGTTGC TAACATCAAC AATTAACAGC 
ATCAAAAAAG TATTGAAGAA AAGAGGTGAT GATTTTGAAA CCGTCTCCTT CTGGCTCTCT 
AACACATGCC GATTTTTGCA CTGCTTGAAA CAGTACAGTG GAGAAGAGGG CTTTATGAAG 
CACAACACAT CTCGCCAGAA TGAACACTGC CTCACCAATT TTGACCTGGC TGAGTATCGG 
CAGGTGCTGA GTGACTTGGC CATTCAGATC TACCAGCAGC TCGTGCGGGT GTTAGAGAAC 
ATCCTTCAGC CAATGATTGT CTCAGGCATG CTGGAACATG AAACGATTCA GGGCGTGTCT 
GGGGTGAAGC CCACAGGGTT GAGAAAGCGA ACCTCCAGTA TCGCCGATGA GGGCACCTAC 
ACACTGGACT CCATCCTCCG GCAGCTCAAC TCCTTCCACT CGGTCATGTG TCAGCATGGC 
ATGGACCCTG AACTGATCAA GCAGGTGGTC AAGCAGATGT TCTACATCAT AGGGGCCATC 
ACCCTGAACA ACCTTCTCCT GCGGAAGGAC ATGTGCTCCT GGAGTAAAGG CATGCAGATC 
AGGTACAATG TCAGTCAACT GGAAGAATGG CTGCGTGACA AGAATCTGAT GAATAGTGGG 
GCTAAAGAAA CCCTGGAACC TCTCATTCAG GCTGCTCAAC TTTTGCAAGT GAAAAAGAAA 
ACAGATGATG ATGCAGAAGC CATTTGTTCT ATGTGCAATG CTTTAACTAC TGCCCAGATT 
GTGAAAGTGT TGAATTTGTA TACTCCAGTT AATGAGTTTG AAGAAAGAGT CTCTGTGTCG 
TTCATTCGTA CTATACAGAT GCGTTTACGA GACAGGAAAG ACTCTCCCCA GCTGCTCATG 
GATGCTAAAC ACATCTTTCC TGTCACCTTT CCTTTCAACC CATCTTCCCT CGCACTAGAA 
ACCATCCAGA TTCCAGCCAG CCTCGGCCTG GGCTTCATTT CACGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521610.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519912.2 Homo sapiens myosin VA (MYO5A), transcript variant X6, mRNA.

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CloneID	OHu85433
Clone ID Related Accession (Same CDS sequence)	XM_011521610.2 , XM_011521610.3
Accession Version	XM_011521610.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521610.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GAATACAATG ACAGATTCCA CAATACTTTT GGAAGATGTA 
CAAAAAATGA AAGATAAAGG TGAAATAGCA CAAGCATACA TTGGTTTGAA AGAAACAAAT 
AGATCATCTG CTCTGGATTA CCATGAGTTG AATGAGGATG GAGAGCTGTG GCTGGTTTAT 
GAAGGGTTAA AACAAGCCAA CAGGCTCCTG GAATCCCAGC TGCAGTCACA GAAGAGGAGC 
CATGAGAATG AGGCCGAGGC CCTCCGTGGG GAGATCCAGA GCCTGAAGGA GGAGAACAAC 
CGACAGCAGC AGCTGCTGGC CCAGAACCTG CAGCTGCCCC CAGAGGCCCG CATTGAGGCC 
AGCCTGCAGC ACGAGATCAC CCGGCTGACC AACGAAAACT TGGATTTGAT GGAACAACTT 
GAAAAACAGG ATAAGACGGT CCGTAAACTG AAAAAACAAC TGAAAGTATT TGCCAAAAAA 
ATTGGCGAAC TAGAAGTGGG CCAGATGGAG AACATATCCC CAGGACAGAT CATTGATGAA 
CCCATCCGAC CAGTCAACAT TCCCAGGAAA GAAAAGGATT TCCAAGGGAT GCTGGAATAC 
AAGAAGGAGG ATGAGCAAAA ACTTGTTAAG AACCTGATTC TGGAACTGAA GCCACGTGGT 
GTAGCAGTCA ATTTGATTCC AGGATTACCG GCATATATCC TGTTCATGTG TGTTCGACAT 
GCTGACTACC TGAATGATGA TCAGAAAGTA AGGTCGTTGC TAACATCAAC AATTAACAGC 
ATCAAAAAAG TATTGAAGAA AAGAGGTGAT GATTTTGAAA CCGTCTCCTT CTGGCTCTCT 
AACACATGCC GATTTTTGCA CTGCTTGAAA CAGTACAGTG GAGAAGAGGG CTTTATGAAG 
CACAACACAT CTCGCCAGAA TGAACACTGC CTCACCAATT TTGACCTGGC TGAGTATCGG 
CAGGTGCTGA GTGACTTGGC CATTCAGATC TACCAGCAGC TCGTGCGGGT GTTAGAGAAC 
ATCCTTCAGC CAATGATTGT CTCAGGCATG CTGGAACATG AAACGATTCA GGGCGTGTCT 
GGGGTGAAGC CCACAGGGTT GAGAAAGCGA ACCTCCAGTA TCGCCGATGA GGGCACCTAC 
ACACTGGACT CCATCCTCCG GCAGCTCAAC TCCTTCCACT CGGTCATGTG TCAGCATGGC 
ATGGACCCTG AACTGATCAA GCAGGTGGTC AAGCAGATGT TCTACATCAT AGGGGCCATC 
ACCCTGAACA ACCTTCTCCT GCGGAAGGAC ATGTGCTCCT GGAGTAAAGG CATGCAGATC 
AGGTACAATG TCAGTCAACT GGAAGAATGG CTGCGTGACA AGAATCTGAT GAATAGTGGG 
GCTAAAGAAA CCCTGGAACC TCTCATTCAG GCTGCTCAAC TTTTGCAAGT GAAAAAGAAA 
ACAGATGATG ATGCAGAAGC CATTTGTTCT ATGTGCAATG CTTTAACTAC TGCCCAGATT 
GTGAAAGTGT TGAATTTGTA TACTCCAGTT AATGAGTTTG AAGAAAGAGT CTCTGTGTCG 
TTCATTCGTA CTATACAGAT GCGTTTACGA GACAGGAAAG ACTCTCCCCA GCTGCTCATG 
GATGCTAAAC ACATCTTTCC TGTCACCTTT CCTTTCAACC CATCTTCCCT CGCACTAGAA 
ACCATCCAGA TTCCAGCCAG CCTCGGCCTG GGCTTCATTT CACGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519912.2
CDS	8..5638
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521610.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521610.3

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GAATACAATG ACAGATTCCA CAATACTTTT GGAAGATGTA 
CAAAAAATGA AAGATAAAGG TGAAATAGCA CAAGCATACA TTGGTTTGAA AGAAACAAAT 
AGATCATCTG CTCTGGATTA CCATGAGTTG AATGAGGATG GAGAGCTGTG GCTGGTTTAT 
GAAGGGTTAA AACAAGCCAA CAGGCTCCTG GAATCCCAGC TGCAGTCACA GAAGAGGAGC 
CATGAGAATG AGGCCGAGGC CCTCCGTGGG GAGATCCAGA GCCTGAAGGA GGAGAACAAC 
CGACAGCAGC AGCTGCTGGC CCAGAACCTG CAGCTGCCCC CAGAGGCCCG CATTGAGGCC 
AGCCTGCAGC ACGAGATCAC CCGGCTGACC AACGAAAACT TGGATTTGAT GGAACAACTT 
GAAAAACAGG ATAAGACGGT CCGTAAACTG AAAAAACAAC TGAAAGTATT TGCCAAAAAA 
ATTGGCGAAC TAGAAGTGGG CCAGATGGAG AACATATCCC CAGGACAGAT CATTGATGAA 
CCCATCCGAC CAGTCAACAT TCCCAGGAAA GAAAAGGATT TCCAAGGGAT GCTGGAATAC 
AAGAAGGAGG ATGAGCAAAA ACTTGTTAAG AACCTGATTC TGGAACTGAA GCCACGTGGT 
GTAGCAGTCA ATTTGATTCC AGGATTACCG GCATATATCC TGTTCATGTG TGTTCGACAT 
GCTGACTACC TGAATGATGA TCAGAAAGTA AGGTCGTTGC TAACATCAAC AATTAACAGC 
ATCAAAAAAG TATTGAAGAA AAGAGGTGAT GATTTTGAAA CCGTCTCCTT CTGGCTCTCT 
AACACATGCC GATTTTTGCA CTGCTTGAAA CAGTACAGTG GAGAAGAGGG CTTTATGAAG 
CACAACACAT CTCGCCAGAA TGAACACTGC CTCACCAATT TTGACCTGGC TGAGTATCGG 
CAGGTGCTGA GTGACTTGGC CATTCAGATC TACCAGCAGC TCGTGCGGGT GTTAGAGAAC 
ATCCTTCAGC CAATGATTGT CTCAGGCATG CTGGAACATG AAACGATTCA GGGCGTGTCT 
GGGGTGAAGC CCACAGGGTT GAGAAAGCGA ACCTCCAGTA TCGCCGATGA GGGCACCTAC 
ACACTGGACT CCATCCTCCG GCAGCTCAAC TCCTTCCACT CGGTCATGTG TCAGCATGGC 
ATGGACCCTG AACTGATCAA GCAGGTGGTC AAGCAGATGT TCTACATCAT AGGGGCCATC 
ACCCTGAACA ACCTTCTCCT GCGGAAGGAC ATGTGCTCCT GGAGTAAAGG CATGCAGATC 
AGGTACAATG TCAGTCAACT GGAAGAATGG CTGCGTGACA AGAATCTGAT GAATAGTGGG 
GCTAAAGAAA CCCTGGAACC TCTCATTCAG GCTGCTCAAC TTTTGCAAGT GAAAAAGAAA 
ACAGATGATG ATGCAGAAGC CATTTGTTCT ATGTGCAATG CTTTAACTAC TGCCCAGATT 
GTGAAAGTGT TGAATTTGTA TACTCCAGTT AATGAGTTTG AAGAAAGAGT CTCTGTGTCG 
TTCATTCGTA CTATACAGAT GCGTTTACGA GACAGGAAAG ACTCTCCCCA GCTGCTCATG 
GATGCTAAAC ACATCTTTCC TGTCACCTTT CCTTTCAACC CATCTTCCCT CGCACTAGAA 
ACCATCCAGA TTCCAGCCAG CCTCGGCCTG GGCTTCATTT CACGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_017022227.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877716.1 Homo sapiens myosin VA (MYO5A), transcript variant X7, mRNA.

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CloneID	OHu85434
Clone ID Related Accession (Same CDS sequence)	XM_017022227.1 , XM_017022227.2
Accession Version	XM_017022227.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAATTAGTGA ATCTCATCAA ATGGGAATTT TCCGAATACT TGCTGGCATC 
CTTCACTTAG GCAATGTTGG ATTTACATCC CGAGATGCAG ACAGCTGCAC AATACCTCCC 
AAGCATGAAC CTCTCTGCAT CTTCTGTGAA CTCATGGGTG TGGACTATGA GGAGATGTGT 
CACTGGCTCT GCCATCGGAA ACTGGCTACT GCCACAGAGA CATACATCAA GCCCATCTCC 
AAGCTGCAGG CCACGAATGC CCGCGATGCT TTGGCCAAGC ACATCTATGC CAAGCTCTTT 
AACTGGATTG TAGATAATGT CAATCAGGCT CTCCATTCTG CTGTCAAACA GCACTCTTTT 
ATTGGTGTGC TAGACATTTA CGGATTTGAA ACATTTGAGA TAAATAGTTT TGAACAGTTT 
TGCATAAATT ATGCAAATGA AAAACTACAG CAACAATTCA ATATGCATGT CTTCAAATTG 
GAGCAAGAAG AATATATGAA GGAACAAATT CCATGGACAC TCATAGATTT TTATGATAAT 
CAGCCTTGTA TTAATCTTAT AGAATCAAAA CTAGGCATTC TAGATTTACT GGATGAGGAA 
TGCAAGATGC CTAAAGGCAC AGATGACACC TGGGCCCAAA AATTGTACAA CACACATTTG 
AACAAATGTG CACTCTTTGA AAAGCCTCGT CTATCAAACA AAGCTTTCAT CATCCAACAT 
TTTGCTGACA AAGTGGAATA CCAGTGTGAA GGATTTCTCG AAAAGAATAA AGACACCGTT 
TTTGAAGAAC AAATTAAAGT TCTTAAATCA AGCAAGTTTA AGATGCTACC AGAACTATTT 
CAAGATGATG AGAAGGCCAT CAGTCCAACT TCAGCCACCT CCTCAGGGCG CACACCCCTC 
ACACGAACTC CTGCAAAGCC CACCAAAGGC AGACCAGGCC AAATGGCCAA AGAGCACAAG 
AAAACAGTGG GGCATCAGTT CAGAAACTCC CTGCACCTGC TTATGGAGAC ACTCAATGCC 
ACTACCCCTC ACTATGTGCG CTGTATCAAG CCTAATGACT TCAAGTTCCC ATTCACGTTT 
GATGAGAAGA GGGCAGTGCA GCAGCTGAGA GCATGTGGTG TCCTGGAAAC CATCCGAATC 
AGTGCGGCCG GTTTCCCCTC ACGGTGGACT TACCAAGAAT TTTTCAGCCG CTACCGTGTC 
CTAATGAAGC AGAAAGATGT GCTGAGTGAC AGAAAGCAAA CATGCAAGAA TGTGTTAGAG 
AAACTGATAC TGGACAAGGA CAAATACCAG TTTGGTAAGA CAAAGATCTT TTTCCGTGCC 
GGTCAAGTGG CCTATCTAGA AAAATTGAGA GCTGACAAAC TGAGAGCTGC CTGCATCCGG 
ATCCAGAAGA CCATCCGAGG GTGGCTGCTG AGAAAGAAGT ACCTACGCAT GCGGAAGGCA 
GCCATCACCA TGCAGAGATA CGTGCGGGGC TACCAGGCCC GATGCTATGC TAAGTTTCTG 
CGCAGAACCA AGGCAGCAAC CATCATTCAA AAGTACTGGC GCATGTATGT GGTCCGCAGG 
AGGTACAAGA TTAGACGAGC TGCCACTATC GTTCTTCAGT CTTACTTGCG AGGCTTCTTG 
GCCAGAAATA GGTATCGCAA GATACTCCGT GAGCACAAAG CAGTCATCAT TCAGAAGCGA 
GTCCGGGGCT GGCTGGCCCG CACACACTAC AAGAGGAGCA TGCATGCCAT CATCTACCTT 
CAGTGCTGCT TCAGGCGGAT GATGGCCAAG CGTGAGCTAA AGAAGCTCAA AATCGAGGCT 
CGCTCAGTGG AGCGCTATAA GAAGCTGCAC ATCGGCATGG AGAACAAGAT CATGCAGCTG 
CAGCGCAAAG TTGATGAGCA GAACAAAGAC TACAAATGCC TTGTGGAGAA ACTAACCAAT 
CTGGAAGGAA TATACAACTC TGAGACTGAG AAACTACGAA GTGACTTAGA ACGTCTTCAA 
CTAAGTGAAG AGGAAGCGAA AGTTGCCACT GGGCGGGTCC TTAGTCTGCA GGAAGAAATT 
GCCAAGCTCC GGAAAGACCT GGAGCAAACT CGTTCAGAGA AAAAATGCAT TGAGGAACAT 
GCAGATCGAT ACAAACAAGA AACAGAGCAG CTGGTATCAA ATCTGAAGGA AGAAAATACT 
TTGCTGAAGC AAGAAAAAGA AGCCCTCAAT CACCGCATCG TGCAGCAGGC TAAGGAGATG 
ACAGAAACTA TGGAGAAGAA GTTAGTAGAA GAAACGAAAC AACTGGAACT CGACCTTAAT 
GATGAAAGGC TGAGATATCA GAACCTTCTG AATGAGTTCA GTCGCCTGGA AGAAAGATAT 
GATGACCTCA AGGAAGAGAT GACCCTTATG GTGCATGTGC CTAAGCCTGG ACACAAGAGA 
ACAGACTCCA CCCACAGCAG CAACGAGTCT GAATATATCT TTAGCTCTGA AATTGCAGAA 
ATGGAAGACA TTCCATCAAG GACAGAGGAA CCAAGTGAGA AGAAGGTACC TCTGGACATG 
TCATTGTTCC TTAAGCTCCA GAAGCGGGTC ACAGAGCTGG AGCAGGAGAA GCAGGTGATG 
CAGGATGAGC TGGACCGCAA GGAGGAGCAG GTGCTCCGCA GCAAGGCCAA GGAAGAAGAA 
AGACCACAAA TTAGAGGTGC AGAACTGGAA TATGAGTCAC TCAAGCGTCA AGAACTAGAA 
TCAGAAAACA AAAAACTGAA GAATGAGCTA AATGAGTTGC GCAAGGCCCT CAGTGAGAAA 
AGTGCCCCAG AGGTGACCGC CCCAGGTGCA CCTGCCTACC GTGTCCTCAT GGAGCAGCTG 
ACCTCTGTGA GCGAGGAGCT TGATGTCCGC AAGGAGGAAG TCCTCATCTT AAGGTCTCAA 
CTGGTGAGCC AGAAAGAGGC CATCCAACCC AAGGATGACA AGAATACAAT GACAGATTCC 
ACAATACTTT TGGAAGATGT ACAAAAAATG AAAGATAAAG GTGAAATAGC ACAAGCATAC 
ATTGGTTTGA AAGAAACAAA TAGATCATCT GCTCTGGATT ACCATGAGTT GAATGAGGAT 
GGAGAGCTGT GGCTGGTTTA TGAAGGGTTA AAACAAGCCA ACAGGCTCCT GGAATCCCAG 
CTGCAGTCAC AGAAGAGGAG CCATGAGAAT GAGGCCGAGG CCCTCCGTGG GGAGATCCAG 
AGCCTGAAGG AGGAGAACAA CCGACAGCAG CAGCTGCTGG CCCAGAACCT GCAGCTGCCC 
CCAGAGGCCC GCATTGAGGC CAGCCTGCAG CACGAGATCA CCCGGCTGAC CAACGAAAAC 
TTGTATTTTG AGGAATTATA TGCAGATGAC CCTAAGAAGT ATCAATCATA TCGGATTTCC 
CTTTACAAAC GGATGATTGA TTTGATGGAA CAACTTGAAA AACAGGATAA GACGGTCCGT 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GCGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCCCCAGG ACAGATCATT GATGAACCCA TCCGACCAGT CAACATTCCC 
AGGAAAGAAA AGGATTTCCA AGGGATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTTAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGA 
TTACCGGCAT ATATCCTGTT CATGTGTGTT CGACATGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AACAGCATCA AAAAAGTATT GAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTCTGG CTCTCTAACA CATGCCGATT TTTGCACTGC 
TTGAAACAGT ACAGTGGAGA AGAGGGCTTT ATGAAGCACA ACACATCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CCTGGCTGAG TATCGGCAGG TGCTGAGTGA CTTGGCCATT 
CAGATCTACC AGCAGCTCGT GCGGGTGTTA GAGAACATCC TTCAGCCAAT GATTGTCTCA 
GGCATGCTGG AACATGAAAC GATTCAGGGC GTGTCTGGGG TGAAGCCCAC AGGGTTGAGA 
AAGCGAACCT CCAGTATCGC CGATGAGGGC ACCTACACAC TGGACTCCAT CCTCCGGCAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CATGGCATGG ACCCTGAACT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCATAGGG GCCATCACCC TGAACAACCT TCTCCTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTGGAA 
GAATGGCTGC GTGACAAGAA TCTGATGAAT AGTGGGGCTA AAGAAACCCT GGAACCTCTC 
ATTCAGGCTG CTCAACTTTT GCAAGTGAAA AAGAAAACAG ATGATGATGC AGAAGCCATT 
TGTTCTATGT GCAATGCTTT AACTACTGCC CAGATTGTGA AAGTGTTGAA TTTGTATACT 
CCAGTTAATG AGTTTGAAGA AAGAGTCTCT GTGTCGTTCA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCCCAGCTG CTCATGGATG CTAAACACAT CTTTCCTGTC 
ACCTTTCCTT TCAACCCATC TTCCCTCGCA CTAGAAACCA TCCAGATTCC AGCCAGCCTC 
GGCCTGGGCT TCATTTCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877716.1
CDS	8..5614
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_017022227.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022227.1

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAATTAGTGA ATCTCATCAA ATGGGAATTT TCCGAATACT TGCTGGCATC 
CTTCACTTAG GCAATGTTGG ATTTACATCC CGAGATGCAG ACAGCTGCAC AATACCTCCC 
AAGCATGAAC CTCTCTGCAT CTTCTGTGAA CTCATGGGTG TGGACTATGA GGAGATGTGT 
CACTGGCTCT GCCATCGGAA ACTGGCTACT GCCACAGAGA CATACATCAA GCCCATCTCC 
AAGCTGCAGG CCACGAATGC CCGCGATGCT TTGGCCAAGC ACATCTATGC CAAGCTCTTT 
AACTGGATTG TAGATAATGT CAATCAGGCT CTCCATTCTG CTGTCAAACA GCACTCTTTT 
ATTGGTGTGC TAGACATTTA CGGATTTGAA ACATTTGAGA TAAATAGTTT TGAACAGTTT 
TGCATAAATT ATGCAAATGA AAAACTACAG CAACAATTCA ATATGCATGT CTTCAAATTG 
GAGCAAGAAG AATATATGAA GGAACAAATT CCATGGACAC TCATAGATTT TTATGATAAT 
CAGCCTTGTA TTAATCTTAT AGAATCAAAA CTAGGCATTC TAGATTTACT GGATGAGGAA 
TGCAAGATGC CTAAAGGCAC AGATGACACC TGGGCCCAAA AATTGTACAA CACACATTTG 
AACAAATGTG CACTCTTTGA AAAGCCTCGT CTATCAAACA AAGCTTTCAT CATCCAACAT 
TTTGCTGACA AAGTGGAATA CCAGTGTGAA GGATTTCTCG AAAAGAATAA AGACACCGTT 
TTTGAAGAAC AAATTAAAGT TCTTAAATCA AGCAAGTTTA AGATGCTACC AGAACTATTT 
CAAGATGATG AGAAGGCCAT CAGTCCAACT TCAGCCACCT CCTCAGGGCG CACACCCCTC 
ACACGAACTC CTGCAAAGCC CACCAAAGGC AGACCAGGCC AAATGGCCAA AGAGCACAAG 
AAAACAGTGG GGCATCAGTT CAGAAACTCC CTGCACCTGC TTATGGAGAC ACTCAATGCC 
ACTACCCCTC ACTATGTGCG CTGTATCAAG CCTAATGACT TCAAGTTCCC ATTCACGTTT 
GATGAGAAGA GGGCAGTGCA GCAGCTGAGA GCATGTGGTG TCCTGGAAAC CATCCGAATC 
AGTGCGGCCG GTTTCCCCTC ACGGTGGACT TACCAAGAAT TTTTCAGCCG CTACCGTGTC 
CTAATGAAGC AGAAAGATGT GCTGAGTGAC AGAAAGCAAA CATGCAAGAA TGTGTTAGAG 
AAACTGATAC TGGACAAGGA CAAATACCAG TTTGGTAAGA CAAAGATCTT TTTCCGTGCC 
GGTCAAGTGG CCTATCTAGA AAAATTGAGA GCTGACAAAC TGAGAGCTGC CTGCATCCGG 
ATCCAGAAGA CCATCCGAGG GTGGCTGCTG AGAAAGAAGT ACCTACGCAT GCGGAAGGCA 
GCCATCACCA TGCAGAGATA CGTGCGGGGC TACCAGGCCC GATGCTATGC TAAGTTTCTG 
CGCAGAACCA AGGCAGCAAC CATCATTCAA AAGTACTGGC GCATGTATGT GGTCCGCAGG 
AGGTACAAGA TTAGACGAGC TGCCACTATC GTTCTTCAGT CTTACTTGCG AGGCTTCTTG 
GCCAGAAATA GGTATCGCAA GATACTCCGT GAGCACAAAG CAGTCATCAT TCAGAAGCGA 
GTCCGGGGCT GGCTGGCCCG CACACACTAC AAGAGGAGCA TGCATGCCAT CATCTACCTT 
CAGTGCTGCT TCAGGCGGAT GATGGCCAAG CGTGAGCTAA AGAAGCTCAA AATCGAGGCT 
CGCTCAGTGG AGCGCTATAA GAAGCTGCAC ATCGGCATGG AGAACAAGAT CATGCAGCTG 
CAGCGCAAAG TTGATGAGCA GAACAAAGAC TACAAATGCC TTGTGGAGAA ACTAACCAAT 
CTGGAAGGAA TATACAACTC TGAGACTGAG AAACTACGAA GTGACTTAGA ACGTCTTCAA 
CTAAGTGAAG AGGAAGCGAA AGTTGCCACT GGGCGGGTCC TTAGTCTGCA GGAAGAAATT 
GCCAAGCTCC GGAAAGACCT GGAGCAAACT CGTTCAGAGA AAAAATGCAT TGAGGAACAT 
GCAGATCGAT ACAAACAAGA AACAGAGCAG CTGGTATCAA ATCTGAAGGA AGAAAATACT 
TTGCTGAAGC AAGAAAAAGA AGCCCTCAAT CACCGCATCG TGCAGCAGGC TAAGGAGATG 
ACAGAAACTA TGGAGAAGAA GTTAGTAGAA GAAACGAAAC AACTGGAACT CGACCTTAAT 
GATGAAAGGC TGAGATATCA GAACCTTCTG AATGAGTTCA GTCGCCTGGA AGAAAGATAT 
GATGACCTCA AGGAAGAGAT GACCCTTATG GTGCATGTGC CTAAGCCTGG ACACAAGAGA 
ACAGACTCCA CCCACAGCAG CAACGAGTCT GAATATATCT TTAGCTCTGA AATTGCAGAA 
ATGGAAGACA TTCCATCAAG GACAGAGGAA CCAAGTGAGA AGAAGGTACC TCTGGACATG 
TCATTGTTCC TTAAGCTCCA GAAGCGGGTC ACAGAGCTGG AGCAGGAGAA GCAGGTGATG 
CAGGATGAGC TGGACCGCAA GGAGGAGCAG GTGCTCCGCA GCAAGGCCAA GGAAGAAGAA 
AGACCACAAA TTAGAGGTGC AGAACTGGAA TATGAGTCAC TCAAGCGTCA AGAACTAGAA 
TCAGAAAACA AAAAACTGAA GAATGAGCTA AATGAGTTGC GCAAGGCCCT CAGTGAGAAA 
AGTGCCCCAG AGGTGACCGC CCCAGGTGCA CCTGCCTACC GTGTCCTCAT GGAGCAGCTG 
ACCTCTGTGA GCGAGGAGCT TGATGTCCGC AAGGAGGAAG TCCTCATCTT AAGGTCTCAA 
CTGGTGAGCC AGAAAGAGGC CATCCAACCC AAGGATGACA AGAATACAAT GACAGATTCC 
ACAATACTTT TGGAAGATGT ACAAAAAATG AAAGATAAAG GTGAAATAGC ACAAGCATAC 
ATTGGTTTGA AAGAAACAAA TAGATCATCT GCTCTGGATT ACCATGAGTT GAATGAGGAT 
GGAGAGCTGT GGCTGGTTTA TGAAGGGTTA AAACAAGCCA ACAGGCTCCT GGAATCCCAG 
CTGCAGTCAC AGAAGAGGAG CCATGAGAAT GAGGCCGAGG CCCTCCGTGG GGAGATCCAG 
AGCCTGAAGG AGGAGAACAA CCGACAGCAG CAGCTGCTGG CCCAGAACCT GCAGCTGCCC 
CCAGAGGCCC GCATTGAGGC CAGCCTGCAG CACGAGATCA CCCGGCTGAC CAACGAAAAC 
TTGTATTTTG AGGAATTATA TGCAGATGAC CCTAAGAAGT ATCAATCATA TCGGATTTCC 
CTTTACAAAC GGATGATTGA TTTGATGGAA CAACTTGAAA AACAGGATAA GACGGTCCGT 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GCGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCCCCAGG ACAGATCATT GATGAACCCA TCCGACCAGT CAACATTCCC 
AGGAAAGAAA AGGATTTCCA AGGGATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTTAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGA 
TTACCGGCAT ATATCCTGTT CATGTGTGTT CGACATGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AACAGCATCA AAAAAGTATT GAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTCTGG CTCTCTAACA CATGCCGATT TTTGCACTGC 
TTGAAACAGT ACAGTGGAGA AGAGGGCTTT ATGAAGCACA ACACATCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CCTGGCTGAG TATCGGCAGG TGCTGAGTGA CTTGGCCATT 
CAGATCTACC AGCAGCTCGT GCGGGTGTTA GAGAACATCC TTCAGCCAAT GATTGTCTCA 
GGCATGCTGG AACATGAAAC GATTCAGGGC GTGTCTGGGG TGAAGCCCAC AGGGTTGAGA 
AAGCGAACCT CCAGTATCGC CGATGAGGGC ACCTACACAC TGGACTCCAT CCTCCGGCAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CATGGCATGG ACCCTGAACT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCATAGGG GCCATCACCC TGAACAACCT TCTCCTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTGGAA 
GAATGGCTGC GTGACAAGAA TCTGATGAAT AGTGGGGCTA AAGAAACCCT GGAACCTCTC 
ATTCAGGCTG CTCAACTTTT GCAAGTGAAA AAGAAAACAG ATGATGATGC AGAAGCCATT 
TGTTCTATGT GCAATGCTTT AACTACTGCC CAGATTGTGA AAGTGTTGAA TTTGTATACT 
CCAGTTAATG AGTTTGAAGA AAGAGTCTCT GTGTCGTTCA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCCCAGCTG CTCATGGATG CTAAACACAT CTTTCCTGTC 
ACCTTTCCTT TCAACCCATC TTCCCTCGCA CTAGAAACCA TCCAGATTCC AGCCAGCCTC 
GGCCTGGGCT TCATTTCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_017022227.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877716.1 Homo sapiens myosin VA (MYO5A), transcript variant X7, mRNA.

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CloneID	OHu85434
Clone ID Related Accession (Same CDS sequence)	XM_017022227.1 , XM_017022227.2
Accession Version	XM_017022227.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022227.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAATTAGTGA ATCTCATCAA ATGGGAATTT TCCGAATACT TGCTGGCATC 
CTTCACTTAG GCAATGTTGG ATTTACATCC CGAGATGCAG ACAGCTGCAC AATACCTCCC 
AAGCATGAAC CTCTCTGCAT CTTCTGTGAA CTCATGGGTG TGGACTATGA GGAGATGTGT 
CACTGGCTCT GCCATCGGAA ACTGGCTACT GCCACAGAGA CATACATCAA GCCCATCTCC 
AAGCTGCAGG CCACGAATGC CCGCGATGCT TTGGCCAAGC ACATCTATGC CAAGCTCTTT 
AACTGGATTG TAGATAATGT CAATCAGGCT CTCCATTCTG CTGTCAAACA GCACTCTTTT 
ATTGGTGTGC TAGACATTTA CGGATTTGAA ACATTTGAGA TAAATAGTTT TGAACAGTTT 
TGCATAAATT ATGCAAATGA AAAACTACAG CAACAATTCA ATATGCATGT CTTCAAATTG 
GAGCAAGAAG AATATATGAA GGAACAAATT CCATGGACAC TCATAGATTT TTATGATAAT 
CAGCCTTGTA TTAATCTTAT AGAATCAAAA CTAGGCATTC TAGATTTACT GGATGAGGAA 
TGCAAGATGC CTAAAGGCAC AGATGACACC TGGGCCCAAA AATTGTACAA CACACATTTG 
AACAAATGTG CACTCTTTGA AAAGCCTCGT CTATCAAACA AAGCTTTCAT CATCCAACAT 
TTTGCTGACA AAGTGGAATA CCAGTGTGAA GGATTTCTCG AAAAGAATAA AGACACCGTT 
TTTGAAGAAC AAATTAAAGT TCTTAAATCA AGCAAGTTTA AGATGCTACC AGAACTATTT 
CAAGATGATG AGAAGGCCAT CAGTCCAACT TCAGCCACCT CCTCAGGGCG CACACCCCTC 
ACACGAACTC CTGCAAAGCC CACCAAAGGC AGACCAGGCC AAATGGCCAA AGAGCACAAG 
AAAACAGTGG GGCATCAGTT CAGAAACTCC CTGCACCTGC TTATGGAGAC ACTCAATGCC 
ACTACCCCTC ACTATGTGCG CTGTATCAAG CCTAATGACT TCAAGTTCCC ATTCACGTTT 
GATGAGAAGA GGGCAGTGCA GCAGCTGAGA GCATGTGGTG TCCTGGAAAC CATCCGAATC 
AGTGCGGCCG GTTTCCCCTC ACGGTGGACT TACCAAGAAT TTTTCAGCCG CTACCGTGTC 
CTAATGAAGC AGAAAGATGT GCTGAGTGAC AGAAAGCAAA CATGCAAGAA TGTGTTAGAG 
AAACTGATAC TGGACAAGGA CAAATACCAG TTTGGTAAGA CAAAGATCTT TTTCCGTGCC 
GGTCAAGTGG CCTATCTAGA AAAATTGAGA GCTGACAAAC TGAGAGCTGC CTGCATCCGG 
ATCCAGAAGA CCATCCGAGG GTGGCTGCTG AGAAAGAAGT ACCTACGCAT GCGGAAGGCA 
GCCATCACCA TGCAGAGATA CGTGCGGGGC TACCAGGCCC GATGCTATGC TAAGTTTCTG 
CGCAGAACCA AGGCAGCAAC CATCATTCAA AAGTACTGGC GCATGTATGT GGTCCGCAGG 
AGGTACAAGA TTAGACGAGC TGCCACTATC GTTCTTCAGT CTTACTTGCG AGGCTTCTTG 
GCCAGAAATA GGTATCGCAA GATACTCCGT GAGCACAAAG CAGTCATCAT TCAGAAGCGA 
GTCCGGGGCT GGCTGGCCCG CACACACTAC AAGAGGAGCA TGCATGCCAT CATCTACCTT 
CAGTGCTGCT TCAGGCGGAT GATGGCCAAG CGTGAGCTAA AGAAGCTCAA AATCGAGGCT 
CGCTCAGTGG AGCGCTATAA GAAGCTGCAC ATCGGCATGG AGAACAAGAT CATGCAGCTG 
CAGCGCAAAG TTGATGAGCA GAACAAAGAC TACAAATGCC TTGTGGAGAA ACTAACCAAT 
CTGGAAGGAA TATACAACTC TGAGACTGAG AAACTACGAA GTGACTTAGA ACGTCTTCAA 
CTAAGTGAAG AGGAAGCGAA AGTTGCCACT GGGCGGGTCC TTAGTCTGCA GGAAGAAATT 
GCCAAGCTCC GGAAAGACCT GGAGCAAACT CGTTCAGAGA AAAAATGCAT TGAGGAACAT 
GCAGATCGAT ACAAACAAGA AACAGAGCAG CTGGTATCAA ATCTGAAGGA AGAAAATACT 
TTGCTGAAGC AAGAAAAAGA AGCCCTCAAT CACCGCATCG TGCAGCAGGC TAAGGAGATG 
ACAGAAACTA TGGAGAAGAA GTTAGTAGAA GAAACGAAAC AACTGGAACT CGACCTTAAT 
GATGAAAGGC TGAGATATCA GAACCTTCTG AATGAGTTCA GTCGCCTGGA AGAAAGATAT 
GATGACCTCA AGGAAGAGAT GACCCTTATG GTGCATGTGC CTAAGCCTGG ACACAAGAGA 
ACAGACTCCA CCCACAGCAG CAACGAGTCT GAATATATCT TTAGCTCTGA AATTGCAGAA 
ATGGAAGACA TTCCATCAAG GACAGAGGAA CCAAGTGAGA AGAAGGTACC TCTGGACATG 
TCATTGTTCC TTAAGCTCCA GAAGCGGGTC ACAGAGCTGG AGCAGGAGAA GCAGGTGATG 
CAGGATGAGC TGGACCGCAA GGAGGAGCAG GTGCTCCGCA GCAAGGCCAA GGAAGAAGAA 
AGACCACAAA TTAGAGGTGC AGAACTGGAA TATGAGTCAC TCAAGCGTCA AGAACTAGAA 
TCAGAAAACA AAAAACTGAA GAATGAGCTA AATGAGTTGC GCAAGGCCCT CAGTGAGAAA 
AGTGCCCCAG AGGTGACCGC CCCAGGTGCA CCTGCCTACC GTGTCCTCAT GGAGCAGCTG 
ACCTCTGTGA GCGAGGAGCT TGATGTCCGC AAGGAGGAAG TCCTCATCTT AAGGTCTCAA 
CTGGTGAGCC AGAAAGAGGC CATCCAACCC AAGGATGACA AGAATACAAT GACAGATTCC 
ACAATACTTT TGGAAGATGT ACAAAAAATG AAAGATAAAG GTGAAATAGC ACAAGCATAC 
ATTGGTTTGA AAGAAACAAA TAGATCATCT GCTCTGGATT ACCATGAGTT GAATGAGGAT 
GGAGAGCTGT GGCTGGTTTA TGAAGGGTTA AAACAAGCCA ACAGGCTCCT GGAATCCCAG 
CTGCAGTCAC AGAAGAGGAG CCATGAGAAT GAGGCCGAGG CCCTCCGTGG GGAGATCCAG 
AGCCTGAAGG AGGAGAACAA CCGACAGCAG CAGCTGCTGG CCCAGAACCT GCAGCTGCCC 
CCAGAGGCCC GCATTGAGGC CAGCCTGCAG CACGAGATCA CCCGGCTGAC CAACGAAAAC 
TTGTATTTTG AGGAATTATA TGCAGATGAC CCTAAGAAGT ATCAATCATA TCGGATTTCC 
CTTTACAAAC GGATGATTGA TTTGATGGAA CAACTTGAAA AACAGGATAA GACGGTCCGT 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GCGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCCCCAGG ACAGATCATT GATGAACCCA TCCGACCAGT CAACATTCCC 
AGGAAAGAAA AGGATTTCCA AGGGATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTTAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGA 
TTACCGGCAT ATATCCTGTT CATGTGTGTT CGACATGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AACAGCATCA AAAAAGTATT GAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTCTGG CTCTCTAACA CATGCCGATT TTTGCACTGC 
TTGAAACAGT ACAGTGGAGA AGAGGGCTTT ATGAAGCACA ACACATCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CCTGGCTGAG TATCGGCAGG TGCTGAGTGA CTTGGCCATT 
CAGATCTACC AGCAGCTCGT GCGGGTGTTA GAGAACATCC TTCAGCCAAT GATTGTCTCA 
GGCATGCTGG AACATGAAAC GATTCAGGGC GTGTCTGGGG TGAAGCCCAC AGGGTTGAGA 
AAGCGAACCT CCAGTATCGC CGATGAGGGC ACCTACACAC TGGACTCCAT CCTCCGGCAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CATGGCATGG ACCCTGAACT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCATAGGG GCCATCACCC TGAACAACCT TCTCCTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTGGAA 
GAATGGCTGC GTGACAAGAA TCTGATGAAT AGTGGGGCTA AAGAAACCCT GGAACCTCTC 
ATTCAGGCTG CTCAACTTTT GCAAGTGAAA AAGAAAACAG ATGATGATGC AGAAGCCATT 
TGTTCTATGT GCAATGCTTT AACTACTGCC CAGATTGTGA AAGTGTTGAA TTTGTATACT 
CCAGTTAATG AGTTTGAAGA AAGAGTCTCT GTGTCGTTCA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCCCAGCTG CTCATGGATG CTAAACACAT CTTTCCTGTC 
ACCTTTCCTT TCAACCCATC TTCCCTCGCA CTAGAAACCA TCCAGATTCC AGCCAGCCTC 
GGCCTGGGCT TCATTTCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877716.1
CDS	8..5614
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_017022227.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022227.2

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAATTAGTGA ATCTCATCAA ATGGGAATTT TCCGAATACT TGCTGGCATC 
CTTCACTTAG GCAATGTTGG ATTTACATCC CGAGATGCAG ACAGCTGCAC AATACCTCCC 
AAGCATGAAC CTCTCTGCAT CTTCTGTGAA CTCATGGGTG TGGACTATGA GGAGATGTGT 
CACTGGCTCT GCCATCGGAA ACTGGCTACT GCCACAGAGA CATACATCAA GCCCATCTCC 
AAGCTGCAGG CCACGAATGC CCGCGATGCT TTGGCCAAGC ACATCTATGC CAAGCTCTTT 
AACTGGATTG TAGATAATGT CAATCAGGCT CTCCATTCTG CTGTCAAACA GCACTCTTTT 
ATTGGTGTGC TAGACATTTA CGGATTTGAA ACATTTGAGA TAAATAGTTT TGAACAGTTT 
TGCATAAATT ATGCAAATGA AAAACTACAG CAACAATTCA ATATGCATGT CTTCAAATTG 
GAGCAAGAAG AATATATGAA GGAACAAATT CCATGGACAC TCATAGATTT TTATGATAAT 
CAGCCTTGTA TTAATCTTAT AGAATCAAAA CTAGGCATTC TAGATTTACT GGATGAGGAA 
TGCAAGATGC CTAAAGGCAC AGATGACACC TGGGCCCAAA AATTGTACAA CACACATTTG 
AACAAATGTG CACTCTTTGA AAAGCCTCGT CTATCAAACA AAGCTTTCAT CATCCAACAT 
TTTGCTGACA AAGTGGAATA CCAGTGTGAA GGATTTCTCG AAAAGAATAA AGACACCGTT 
TTTGAAGAAC AAATTAAAGT TCTTAAATCA AGCAAGTTTA AGATGCTACC AGAACTATTT 
CAAGATGATG AGAAGGCCAT CAGTCCAACT TCAGCCACCT CCTCAGGGCG CACACCCCTC 
ACACGAACTC CTGCAAAGCC CACCAAAGGC AGACCAGGCC AAATGGCCAA AGAGCACAAG 
AAAACAGTGG GGCATCAGTT CAGAAACTCC CTGCACCTGC TTATGGAGAC ACTCAATGCC 
ACTACCCCTC ACTATGTGCG CTGTATCAAG CCTAATGACT TCAAGTTCCC ATTCACGTTT 
GATGAGAAGA GGGCAGTGCA GCAGCTGAGA GCATGTGGTG TCCTGGAAAC CATCCGAATC 
AGTGCGGCCG GTTTCCCCTC ACGGTGGACT TACCAAGAAT TTTTCAGCCG CTACCGTGTC 
CTAATGAAGC AGAAAGATGT GCTGAGTGAC AGAAAGCAAA CATGCAAGAA TGTGTTAGAG 
AAACTGATAC TGGACAAGGA CAAATACCAG TTTGGTAAGA CAAAGATCTT TTTCCGTGCC 
GGTCAAGTGG CCTATCTAGA AAAATTGAGA GCTGACAAAC TGAGAGCTGC CTGCATCCGG 
ATCCAGAAGA CCATCCGAGG GTGGCTGCTG AGAAAGAAGT ACCTACGCAT GCGGAAGGCA 
GCCATCACCA TGCAGAGATA CGTGCGGGGC TACCAGGCCC GATGCTATGC TAAGTTTCTG 
CGCAGAACCA AGGCAGCAAC CATCATTCAA AAGTACTGGC GCATGTATGT GGTCCGCAGG 
AGGTACAAGA TTAGACGAGC TGCCACTATC GTTCTTCAGT CTTACTTGCG AGGCTTCTTG 
GCCAGAAATA GGTATCGCAA GATACTCCGT GAGCACAAAG CAGTCATCAT TCAGAAGCGA 
GTCCGGGGCT GGCTGGCCCG CACACACTAC AAGAGGAGCA TGCATGCCAT CATCTACCTT 
CAGTGCTGCT TCAGGCGGAT GATGGCCAAG CGTGAGCTAA AGAAGCTCAA AATCGAGGCT 
CGCTCAGTGG AGCGCTATAA GAAGCTGCAC ATCGGCATGG AGAACAAGAT CATGCAGCTG 
CAGCGCAAAG TTGATGAGCA GAACAAAGAC TACAAATGCC TTGTGGAGAA ACTAACCAAT 
CTGGAAGGAA TATACAACTC TGAGACTGAG AAACTACGAA GTGACTTAGA ACGTCTTCAA 
CTAAGTGAAG AGGAAGCGAA AGTTGCCACT GGGCGGGTCC TTAGTCTGCA GGAAGAAATT 
GCCAAGCTCC GGAAAGACCT GGAGCAAACT CGTTCAGAGA AAAAATGCAT TGAGGAACAT 
GCAGATCGAT ACAAACAAGA AACAGAGCAG CTGGTATCAA ATCTGAAGGA AGAAAATACT 
TTGCTGAAGC AAGAAAAAGA AGCCCTCAAT CACCGCATCG TGCAGCAGGC TAAGGAGATG 
ACAGAAACTA TGGAGAAGAA GTTAGTAGAA GAAACGAAAC AACTGGAACT CGACCTTAAT 
GATGAAAGGC TGAGATATCA GAACCTTCTG AATGAGTTCA GTCGCCTGGA AGAAAGATAT 
GATGACCTCA AGGAAGAGAT GACCCTTATG GTGCATGTGC CTAAGCCTGG ACACAAGAGA 
ACAGACTCCA CCCACAGCAG CAACGAGTCT GAATATATCT TTAGCTCTGA AATTGCAGAA 
ATGGAAGACA TTCCATCAAG GACAGAGGAA CCAAGTGAGA AGAAGGTACC TCTGGACATG 
TCATTGTTCC TTAAGCTCCA GAAGCGGGTC ACAGAGCTGG AGCAGGAGAA GCAGGTGATG 
CAGGATGAGC TGGACCGCAA GGAGGAGCAG GTGCTCCGCA GCAAGGCCAA GGAAGAAGAA 
AGACCACAAA TTAGAGGTGC AGAACTGGAA TATGAGTCAC TCAAGCGTCA AGAACTAGAA 
TCAGAAAACA AAAAACTGAA GAATGAGCTA AATGAGTTGC GCAAGGCCCT CAGTGAGAAA 
AGTGCCCCAG AGGTGACCGC CCCAGGTGCA CCTGCCTACC GTGTCCTCAT GGAGCAGCTG 
ACCTCTGTGA GCGAGGAGCT TGATGTCCGC AAGGAGGAAG TCCTCATCTT AAGGTCTCAA 
CTGGTGAGCC AGAAAGAGGC CATCCAACCC AAGGATGACA AGAATACAAT GACAGATTCC 
ACAATACTTT TGGAAGATGT ACAAAAAATG AAAGATAAAG GTGAAATAGC ACAAGCATAC 
ATTGGTTTGA AAGAAACAAA TAGATCATCT GCTCTGGATT ACCATGAGTT GAATGAGGAT 
GGAGAGCTGT GGCTGGTTTA TGAAGGGTTA AAACAAGCCA ACAGGCTCCT GGAATCCCAG 
CTGCAGTCAC AGAAGAGGAG CCATGAGAAT GAGGCCGAGG CCCTCCGTGG GGAGATCCAG 
AGCCTGAAGG AGGAGAACAA CCGACAGCAG CAGCTGCTGG CCCAGAACCT GCAGCTGCCC 
CCAGAGGCCC GCATTGAGGC CAGCCTGCAG CACGAGATCA CCCGGCTGAC CAACGAAAAC 
TTGTATTTTG AGGAATTATA TGCAGATGAC CCTAAGAAGT ATCAATCATA TCGGATTTCC 
CTTTACAAAC GGATGATTGA TTTGATGGAA CAACTTGAAA AACAGGATAA GACGGTCCGT 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GCGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCCCCAGG ACAGATCATT GATGAACCCA TCCGACCAGT CAACATTCCC 
AGGAAAGAAA AGGATTTCCA AGGGATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTTAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGA 
TTACCGGCAT ATATCCTGTT CATGTGTGTT CGACATGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AACAGCATCA AAAAAGTATT GAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTCTGG CTCTCTAACA CATGCCGATT TTTGCACTGC 
TTGAAACAGT ACAGTGGAGA AGAGGGCTTT ATGAAGCACA ACACATCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CCTGGCTGAG TATCGGCAGG TGCTGAGTGA CTTGGCCATT 
CAGATCTACC AGCAGCTCGT GCGGGTGTTA GAGAACATCC TTCAGCCAAT GATTGTCTCA 
GGCATGCTGG AACATGAAAC GATTCAGGGC GTGTCTGGGG TGAAGCCCAC AGGGTTGAGA 
AAGCGAACCT CCAGTATCGC CGATGAGGGC ACCTACACAC TGGACTCCAT CCTCCGGCAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CATGGCATGG ACCCTGAACT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCATAGGG GCCATCACCC TGAACAACCT TCTCCTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTGGAA 
GAATGGCTGC GTGACAAGAA TCTGATGAAT AGTGGGGCTA AAGAAACCCT GGAACCTCTC 
ATTCAGGCTG CTCAACTTTT GCAAGTGAAA AAGAAAACAG ATGATGATGC AGAAGCCATT 
TGTTCTATGT GCAATGCTTT AACTACTGCC CAGATTGTGA AAGTGTTGAA TTTGTATACT 
CCAGTTAATG AGTTTGAAGA AAGAGTCTCT GTGTCGTTCA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCCCAGCTG CTCATGGATG CTAAACACAT CTTTCCTGTC 
ACCTTTCCTT TCAACCCATC TTCCCTCGCA CTAGAAACCA TCCAGATTCC AGCCAGCCTC 
GGCCTGGGCT TCATTTCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521611.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519913.2 Homo sapiens myosin VA (MYO5A), transcript variant X8, mRNA.

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CloneID	OHu85435
Clone ID Related Accession (Same CDS sequence)	XM_011521611.2 , XM_011521611.3
Accession Version	XM_011521611.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521611.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GGCTCCTGGA ATCCCAGCTG CAGTCACAGA AGAGGAGCCA TGAGAATGAG 
GCCGAGGCCC TCCGTGGGGA GATCCAGAGC CTGAAGGAGG AGAACAACCG ACAGCAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACCC GGCTGACCAA CGAAAACTTG GATTTGATGG AACAACTTGA AAAACAGGAT 
AAGACGGTCC GTAAACTGAA AAAACAACTG AAAGTATTTG CCAAAAAAAT TGGCGAACTA 
GAAGTGGGCC AGATGGAGAA CATATCCCCA GGACAGATCA TTGATGAACC CATCCGACCA 
GTCAACATTC CCAGGAAAGA AAAGGATTTC CAAGGGATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTTAAGAA CCTGATTCTG GAACTGAAGC CACGTGGTGT AGCAGTCAAT 
TTGATTCCAG GATTACCGGC ATATATCCTG TTCATGTGTG TTCGACATGC TGACTACCTG 
AATGATGATC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAACAGCAT CAAAAAAGTA 
TTGAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTCT GGCTCTCTAA CACATGCCGA 
TTTTTGCACT GCTTGAAACA GTACAGTGGA GAAGAGGGCT TTATGAAGCA CAACACATCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACCTGGCTG AGTATCGGCA GGTGCTGAGT 
GACTTGGCCA TTCAGATCTA CCAGCAGCTC GTGCGGGTGT TAGAGAACAT CCTTCAGCCA 
ATGATTGTCT CAGGCATGCT GGAACATGAA ACGATTCAGG GCGTGTCTGG GGTGAAGCCC 
ACAGGGTTGA GAAAGCGAAC CTCCAGTATC GCCGATGAGG GCACCTACAC ACTGGACTCC 
ATCCTCCGGC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCATGGCAT GGACCCTGAA 
CTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATCATAG GGGCCATCAC CCTGAACAAC 
CTTCTCCTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTGG AAGAATGGCT GCGTGACAAG AATCTGATGA ATAGTGGGGC TAAAGAAACC 
CTGGAACCTC TCATTCAGGC TGCTCAACTT TTGCAAGTGA AAAAGAAAAC AGATGATGAT 
GCAGAAGCCA TTTGTTCTAT GTGCAATGCT TTAACTACTG CCCAGATTGT GAAAGTGTTG 
AATTTGTATA CTCCAGTTAA TGAGTTTGAA GAAAGAGTCT CTGTGTCGTT CATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCCCAGC TGCTCATGGA TGCTAAACAC 
ATCTTTCCTG TCACCTTTCC TTTCAACCCA TCTTCCCTCG CACTAGAAAC CATCCAGATT 
CCAGCCAGCC TCGGCCTGGG CTTCATTTCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519913.2
CDS	7..5565
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521611.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521611.2

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GGCTCCTGGA ATCCCAGCTG CAGTCACAGA AGAGGAGCCA TGAGAATGAG 
GCCGAGGCCC TCCGTGGGGA GATCCAGAGC CTGAAGGAGG AGAACAACCG ACAGCAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACCC GGCTGACCAA CGAAAACTTG GATTTGATGG AACAACTTGA AAAACAGGAT 
AAGACGGTCC GTAAACTGAA AAAACAACTG AAAGTATTTG CCAAAAAAAT TGGCGAACTA 
GAAGTGGGCC AGATGGAGAA CATATCCCCA GGACAGATCA TTGATGAACC CATCCGACCA 
GTCAACATTC CCAGGAAAGA AAAGGATTTC CAAGGGATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTTAAGAA CCTGATTCTG GAACTGAAGC CACGTGGTGT AGCAGTCAAT 
TTGATTCCAG GATTACCGGC ATATATCCTG TTCATGTGTG TTCGACATGC TGACTACCTG 
AATGATGATC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAACAGCAT CAAAAAAGTA 
TTGAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTCT GGCTCTCTAA CACATGCCGA 
TTTTTGCACT GCTTGAAACA GTACAGTGGA GAAGAGGGCT TTATGAAGCA CAACACATCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACCTGGCTG AGTATCGGCA GGTGCTGAGT 
GACTTGGCCA TTCAGATCTA CCAGCAGCTC GTGCGGGTGT TAGAGAACAT CCTTCAGCCA 
ATGATTGTCT CAGGCATGCT GGAACATGAA ACGATTCAGG GCGTGTCTGG GGTGAAGCCC 
ACAGGGTTGA GAAAGCGAAC CTCCAGTATC GCCGATGAGG GCACCTACAC ACTGGACTCC 
ATCCTCCGGC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCATGGCAT GGACCCTGAA 
CTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATCATAG GGGCCATCAC CCTGAACAAC 
CTTCTCCTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTGG AAGAATGGCT GCGTGACAAG AATCTGATGA ATAGTGGGGC TAAAGAAACC 
CTGGAACCTC TCATTCAGGC TGCTCAACTT TTGCAAGTGA AAAAGAAAAC AGATGATGAT 
GCAGAAGCCA TTTGTTCTAT GTGCAATGCT TTAACTACTG CCCAGATTGT GAAAGTGTTG 
AATTTGTATA CTCCAGTTAA TGAGTTTGAA GAAAGAGTCT CTGTGTCGTT CATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCCCAGC TGCTCATGGA TGCTAAACAC 
ATCTTTCCTG TCACCTTTCC TTTCAACCCA TCTTCCCTCG CACTAGAAAC CATCCAGATT 
CCAGCCAGCC TCGGCCTGGG CTTCATTTCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521611.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519913.2 Homo sapiens myosin VA (MYO5A), transcript variant X8, mRNA.

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CloneID	OHu85435
Clone ID Related Accession (Same CDS sequence)	XM_011521611.2 , XM_011521611.3
Accession Version	XM_011521611.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GGCTCCTGGA ATCCCAGCTG CAGTCACAGA AGAGGAGCCA TGAGAATGAG 
GCCGAGGCCC TCCGTGGGGA GATCCAGAGC CTGAAGGAGG AGAACAACCG ACAGCAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACCC GGCTGACCAA CGAAAACTTG GATTTGATGG AACAACTTGA AAAACAGGAT 
AAGACGGTCC GTAAACTGAA AAAACAACTG AAAGTATTTG CCAAAAAAAT TGGCGAACTA 
GAAGTGGGCC AGATGGAGAA CATATCCCCA GGACAGATCA TTGATGAACC CATCCGACCA 
GTCAACATTC CCAGGAAAGA AAAGGATTTC CAAGGGATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTTAAGAA CCTGATTCTG GAACTGAAGC CACGTGGTGT AGCAGTCAAT 
TTGATTCCAG GATTACCGGC ATATATCCTG TTCATGTGTG TTCGACATGC TGACTACCTG 
AATGATGATC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAACAGCAT CAAAAAAGTA 
TTGAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTCT GGCTCTCTAA CACATGCCGA 
TTTTTGCACT GCTTGAAACA GTACAGTGGA GAAGAGGGCT TTATGAAGCA CAACACATCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACCTGGCTG AGTATCGGCA GGTGCTGAGT 
GACTTGGCCA TTCAGATCTA CCAGCAGCTC GTGCGGGTGT TAGAGAACAT CCTTCAGCCA 
ATGATTGTCT CAGGCATGCT GGAACATGAA ACGATTCAGG GCGTGTCTGG GGTGAAGCCC 
ACAGGGTTGA GAAAGCGAAC CTCCAGTATC GCCGATGAGG GCACCTACAC ACTGGACTCC 
ATCCTCCGGC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCATGGCAT GGACCCTGAA 
CTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATCATAG GGGCCATCAC CCTGAACAAC 
CTTCTCCTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTGG AAGAATGGCT GCGTGACAAG AATCTGATGA ATAGTGGGGC TAAAGAAACC 
CTGGAACCTC TCATTCAGGC TGCTCAACTT TTGCAAGTGA AAAAGAAAAC AGATGATGAT 
GCAGAAGCCA TTTGTTCTAT GTGCAATGCT TTAACTACTG CCCAGATTGT GAAAGTGTTG 
AATTTGTATA CTCCAGTTAA TGAGTTTGAA GAAAGAGTCT CTGTGTCGTT CATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCCCAGC TGCTCATGGA TGCTAAACAC 
ATCTTTCCTG TCACCTTTCC TTTCAACCCA TCTTCCCTCG CACTAGAAAC CATCCAGATT 
CCAGCCAGCC TCGGCCTGGG CTTCATTTCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519913.2
CDS	7..5565
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521611.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521611.3

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GGATGACAAG AATACAATGA CAGATTCCAC AATACTTTTG 
GAAGATGTAC AAAAAATGAA AGATAAAGGT GAAATAGCAC AAGCATACAT TGGTTTGAAA 
GAAACAAATA GGCTCCTGGA ATCCCAGCTG CAGTCACAGA AGAGGAGCCA TGAGAATGAG 
GCCGAGGCCC TCCGTGGGGA GATCCAGAGC CTGAAGGAGG AGAACAACCG ACAGCAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCA GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACCC GGCTGACCAA CGAAAACTTG GATTTGATGG AACAACTTGA AAAACAGGAT 
AAGACGGTCC GTAAACTGAA AAAACAACTG AAAGTATTTG CCAAAAAAAT TGGCGAACTA 
GAAGTGGGCC AGATGGAGAA CATATCCCCA GGACAGATCA TTGATGAACC CATCCGACCA 
GTCAACATTC CCAGGAAAGA AAAGGATTTC CAAGGGATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTTAAGAA CCTGATTCTG GAACTGAAGC CACGTGGTGT AGCAGTCAAT 
TTGATTCCAG GATTACCGGC ATATATCCTG TTCATGTGTG TTCGACATGC TGACTACCTG 
AATGATGATC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAACAGCAT CAAAAAAGTA 
TTGAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTCT GGCTCTCTAA CACATGCCGA 
TTTTTGCACT GCTTGAAACA GTACAGTGGA GAAGAGGGCT TTATGAAGCA CAACACATCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACCTGGCTG AGTATCGGCA GGTGCTGAGT 
GACTTGGCCA TTCAGATCTA CCAGCAGCTC GTGCGGGTGT TAGAGAACAT CCTTCAGCCA 
ATGATTGTCT CAGGCATGCT GGAACATGAA ACGATTCAGG GCGTGTCTGG GGTGAAGCCC 
ACAGGGTTGA GAAAGCGAAC CTCCAGTATC GCCGATGAGG GCACCTACAC ACTGGACTCC 
ATCCTCCGGC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCATGGCAT GGACCCTGAA 
CTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATCATAG GGGCCATCAC CCTGAACAAC 
CTTCTCCTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTGG AAGAATGGCT GCGTGACAAG AATCTGATGA ATAGTGGGGC TAAAGAAACC 
CTGGAACCTC TCATTCAGGC TGCTCAACTT TTGCAAGTGA AAAAGAAAAC AGATGATGAT 
GCAGAAGCCA TTTGTTCTAT GTGCAATGCT TTAACTACTG CCCAGATTGT GAAAGTGTTG 
AATTTGTATA CTCCAGTTAA TGAGTTTGAA GAAAGAGTCT CTGTGTCGTT CATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCCCAGC TGCTCATGGA TGCTAAACAC 
ATCTTTCCTG TCACCTTTCC TTTCAACCCA TCTTCCCTCG CACTAGAAAC CATCCAGATT 
CCAGCCAGCC TCGGCCTGGG CTTCATTTCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521612.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519914.2 Homo sapiens myosin VA (MYO5A), transcript variant X9, mRNA.

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CloneID	OHu85436
Clone ID Related Accession (Same CDS sequence)	XM_011521612.3 , XM_011521612.2
Accession Version	XM_011521612.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521612.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GAATACAATG ACAGATTCCA CAATACTTTT GGAAGATGTA 
CAAAAAATGA AAGATAAAGG TGAAATAGCA CAAGCATACA TTGGTTTGAA AGAAACAAAT 
AGGCTCCTGG AATCCCAGCT GCAGTCACAG AAGAGGAGCC ATGAGAATGA GGCCGAGGCC 
CTCCGTGGGG AGATCCAGAG CCTGAAGGAG GAGAACAACC GACAGCAGCA GCTGCTGGCC 
CAGAACCTGC AGCTGCCCCC AGAGGCCCGC ATTGAGGCCA GCCTGCAGCA CGAGATCACC 
CGGCTGACCA ACGAAAACTT GGATTTGATG GAACAACTTG AAAAACAGGA TAAGACGGTC 
CGTAAACTGA AAAAACAACT GAAAGTATTT GCCAAAAAAA TTGGCGAACT AGAAGTGGGC 
CAGATGGAGA ACATATCCCC AGGACAGATC ATTGATGAAC CCATCCGACC AGTCAACATT 
CCCAGGAAAG AAAAGGATTT CCAAGGGATG CTGGAATACA AGAAGGAGGA TGAGCAAAAA 
CTTGTTAAGA ACCTGATTCT GGAACTGAAG CCACGTGGTG TAGCAGTCAA TTTGATTCCA 
GGATTACCGG CATATATCCT GTTCATGTGT GTTCGACATG CTGACTACCT GAATGATGAT 
CAGAAAGTAA GGTCGTTGCT AACATCAACA ATTAACAGCA TCAAAAAAGT ATTGAAGAAA 
AGAGGTGATG ATTTTGAAAC CGTCTCCTTC TGGCTCTCTA ACACATGCCG ATTTTTGCAC 
TGCTTGAAAC AGTACAGTGG AGAAGAGGGC TTTATGAAGC ACAACACATC TCGCCAGAAT 
GAACACTGCC TCACCAATTT TGACCTGGCT GAGTATCGGC AGGTGCTGAG TGACTTGGCC 
ATTCAGATCT ACCAGCAGCT CGTGCGGGTG TTAGAGAACA TCCTTCAGCC AATGATTGTC 
TCAGGCATGC TGGAACATGA AACGATTCAG GGCGTGTCTG GGGTGAAGCC CACAGGGTTG 
AGAAAGCGAA CCTCCAGTAT CGCCGATGAG GGCACCTACA CACTGGACTC CATCCTCCGG 
CAGCTCAACT CCTTCCACTC GGTCATGTGT CAGCATGGCA TGGACCCTGA ACTGATCAAG 
CAGGTGGTCA AGCAGATGTT CTACATCATA GGGGCCATCA CCCTGAACAA CCTTCTCCTG 
CGGAAGGACA TGTGCTCCTG GAGTAAAGGC ATGCAGATCA GGTACAATGT CAGTCAACTG 
GAAGAATGGC TGCGTGACAA GAATCTGATG AATAGTGGGG CTAAAGAAAC CCTGGAACCT 
CTCATTCAGG CTGCTCAACT TTTGCAAGTG AAAAAGAAAA CAGATGATGA TGCAGAAGCC 
ATTTGTTCTA TGTGCAATGC TTTAACTACT GCCCAGATTG TGAAAGTGTT GAATTTGTAT 
ACTCCAGTTA ATGAGTTTGA AGAAAGAGTC TCTGTGTCGT TCATTCGTAC TATACAGATG 
CGTTTACGAG ACAGGAAAGA CTCTCCCCAG CTGCTCATGG ATGCTAAACA CATCTTTCCT 
GTCACCTTTC CTTTCAACCC ATCTTCCCTC GCACTAGAAA CCATCCAGAT TCCAGCCAGC 
CTCGGCCTGG GCTTCATTTC ACGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519914.2
CDS	7..5556
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521612.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521612.3

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GAATACAATG ACAGATTCCA CAATACTTTT GGAAGATGTA 
CAAAAAATGA AAGATAAAGG TGAAATAGCA CAAGCATACA TTGGTTTGAA AGAAACAAAT 
AGGCTCCTGG AATCCCAGCT GCAGTCACAG AAGAGGAGCC ATGAGAATGA GGCCGAGGCC 
CTCCGTGGGG AGATCCAGAG CCTGAAGGAG GAGAACAACC GACAGCAGCA GCTGCTGGCC 
CAGAACCTGC AGCTGCCCCC AGAGGCCCGC ATTGAGGCCA GCCTGCAGCA CGAGATCACC 
CGGCTGACCA ACGAAAACTT GGATTTGATG GAACAACTTG AAAAACAGGA TAAGACGGTC 
CGTAAACTGA AAAAACAACT GAAAGTATTT GCCAAAAAAA TTGGCGAACT AGAAGTGGGC 
CAGATGGAGA ACATATCCCC AGGACAGATC ATTGATGAAC CCATCCGACC AGTCAACATT 
CCCAGGAAAG AAAAGGATTT CCAAGGGATG CTGGAATACA AGAAGGAGGA TGAGCAAAAA 
CTTGTTAAGA ACCTGATTCT GGAACTGAAG CCACGTGGTG TAGCAGTCAA TTTGATTCCA 
GGATTACCGG CATATATCCT GTTCATGTGT GTTCGACATG CTGACTACCT GAATGATGAT 
CAGAAAGTAA GGTCGTTGCT AACATCAACA ATTAACAGCA TCAAAAAAGT ATTGAAGAAA 
AGAGGTGATG ATTTTGAAAC CGTCTCCTTC TGGCTCTCTA ACACATGCCG ATTTTTGCAC 
TGCTTGAAAC AGTACAGTGG AGAAGAGGGC TTTATGAAGC ACAACACATC TCGCCAGAAT 
GAACACTGCC TCACCAATTT TGACCTGGCT GAGTATCGGC AGGTGCTGAG TGACTTGGCC 
ATTCAGATCT ACCAGCAGCT CGTGCGGGTG TTAGAGAACA TCCTTCAGCC AATGATTGTC 
TCAGGCATGC TGGAACATGA AACGATTCAG GGCGTGTCTG GGGTGAAGCC CACAGGGTTG 
AGAAAGCGAA CCTCCAGTAT CGCCGATGAG GGCACCTACA CACTGGACTC CATCCTCCGG 
CAGCTCAACT CCTTCCACTC GGTCATGTGT CAGCATGGCA TGGACCCTGA ACTGATCAAG 
CAGGTGGTCA AGCAGATGTT CTACATCATA GGGGCCATCA CCCTGAACAA CCTTCTCCTG 
CGGAAGGACA TGTGCTCCTG GAGTAAAGGC ATGCAGATCA GGTACAATGT CAGTCAACTG 
GAAGAATGGC TGCGTGACAA GAATCTGATG AATAGTGGGG CTAAAGAAAC CCTGGAACCT 
CTCATTCAGG CTGCTCAACT TTTGCAAGTG AAAAAGAAAA CAGATGATGA TGCAGAAGCC 
ATTTGTTCTA TGTGCAATGC TTTAACTACT GCCCAGATTG TGAAAGTGTT GAATTTGTAT 
ACTCCAGTTA ATGAGTTTGA AGAAAGAGTC TCTGTGTCGT TCATTCGTAC TATACAGATG 
CGTTTACGAG ACAGGAAAGA CTCTCCCCAG CTGCTCATGG ATGCTAAACA CATCTTTCCT 
GTCACCTTTC CTTTCAACCC ATCTTCCCTC GCACTAGAAA CCATCCAGAT TCCAGCCAGC 
CTCGGCCTGG GCTTCATTTC ACGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_011521612.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519914.2 Homo sapiens myosin VA (MYO5A), transcript variant X9, mRNA.

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CloneID	OHu85436
Clone ID Related Accession (Same CDS sequence)	XM_011521612.3 , XM_011521612.2
Accession Version	XM_011521612.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521612.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GAATACAATG ACAGATTCCA CAATACTTTT GGAAGATGTA 
CAAAAAATGA AAGATAAAGG TGAAATAGCA CAAGCATACA TTGGTTTGAA AGAAACAAAT 
AGGCTCCTGG AATCCCAGCT GCAGTCACAG AAGAGGAGCC ATGAGAATGA GGCCGAGGCC 
CTCCGTGGGG AGATCCAGAG CCTGAAGGAG GAGAACAACC GACAGCAGCA GCTGCTGGCC 
CAGAACCTGC AGCTGCCCCC AGAGGCCCGC ATTGAGGCCA GCCTGCAGCA CGAGATCACC 
CGGCTGACCA ACGAAAACTT GGATTTGATG GAACAACTTG AAAAACAGGA TAAGACGGTC 
CGTAAACTGA AAAAACAACT GAAAGTATTT GCCAAAAAAA TTGGCGAACT AGAAGTGGGC 
CAGATGGAGA ACATATCCCC AGGACAGATC ATTGATGAAC CCATCCGACC AGTCAACATT 
CCCAGGAAAG AAAAGGATTT CCAAGGGATG CTGGAATACA AGAAGGAGGA TGAGCAAAAA 
CTTGTTAAGA ACCTGATTCT GGAACTGAAG CCACGTGGTG TAGCAGTCAA TTTGATTCCA 
GGATTACCGG CATATATCCT GTTCATGTGT GTTCGACATG CTGACTACCT GAATGATGAT 
CAGAAAGTAA GGTCGTTGCT AACATCAACA ATTAACAGCA TCAAAAAAGT ATTGAAGAAA 
AGAGGTGATG ATTTTGAAAC CGTCTCCTTC TGGCTCTCTA ACACATGCCG ATTTTTGCAC 
TGCTTGAAAC AGTACAGTGG AGAAGAGGGC TTTATGAAGC ACAACACATC TCGCCAGAAT 
GAACACTGCC TCACCAATTT TGACCTGGCT GAGTATCGGC AGGTGCTGAG TGACTTGGCC 
ATTCAGATCT ACCAGCAGCT CGTGCGGGTG TTAGAGAACA TCCTTCAGCC AATGATTGTC 
TCAGGCATGC TGGAACATGA AACGATTCAG GGCGTGTCTG GGGTGAAGCC CACAGGGTTG 
AGAAAGCGAA CCTCCAGTAT CGCCGATGAG GGCACCTACA CACTGGACTC CATCCTCCGG 
CAGCTCAACT CCTTCCACTC GGTCATGTGT CAGCATGGCA TGGACCCTGA ACTGATCAAG 
CAGGTGGTCA AGCAGATGTT CTACATCATA GGGGCCATCA CCCTGAACAA CCTTCTCCTG 
CGGAAGGACA TGTGCTCCTG GAGTAAAGGC ATGCAGATCA GGTACAATGT CAGTCAACTG 
GAAGAATGGC TGCGTGACAA GAATCTGATG AATAGTGGGG CTAAAGAAAC CCTGGAACCT 
CTCATTCAGG CTGCTCAACT TTTGCAAGTG AAAAAGAAAA CAGATGATGA TGCAGAAGCC 
ATTTGTTCTA TGTGCAATGC TTTAACTACT GCCCAGATTG TGAAAGTGTT GAATTTGTAT 
ACTCCAGTTA ATGAGTTTGA AGAAAGAGTC TCTGTGTCGT TCATTCGTAC TATACAGATG 
CGTTTACGAG ACAGGAAAGA CTCTCCCCAG CTGCTCATGG ATGCTAAACA CATCTTTCCT 
GTCACCTTTC CTTTCAACCC ATCTTCCCTC GCACTAGAAA CCATCCAGAT TCCAGCCAGC 
CTCGGCCTGG GCTTCATTTC ACGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519914.2
CDS	7..5556
Translation	MCAEMLSIVTVQRQSKIPHLQSMLTCLDETALKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_011521612.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521612.2

ORF Insert Sequence:

ATGTGTGCTG AGATGCTGAG TATTGTAACA GTGCAAAGAC AATCTAAAAT TCCACATTTG 
CAAAGCATGT TAACTTGCCT AGATGAAACA GCTCTTAAGT TTGCCAGGGT TTGGATACCT 
GATCCAGAGG AAGTCTGGAA GTCAGCAGAG CTGCTCAAAG ATTATAAGCC AGGAGATAAA 
GTCCTCCTGC TTCACCTCGA GGAAGGAAAG GATTTGGAAT ACCATCTAGA TCCAAAGACC 
AAGGAGCTGC CTCACTTACG AAATCCTGAC ATACTTGTTG GTGAAAATGA CCTCACAGCC 
CTCAGCTATC TTCATGAGCC TGCTGTGCTC CATAATCTCA GAGTCCGCTT TATTGATTCC 
AAACTTATTT ATACGTATTG TGGTATAGTC CTAGTAGCTA TAAATCCCTA TGAACAGCTG 
CCTATTTATG GAGAAGATAT TATTAATGCA TACAGTGGTC AGAACATGGG TGATATGGAT 
CCACATATCT TTGCAGTAGC TGAAGAAGCT TACAAGCAAA TGGCCAGAGA TGAACGAAAT 
CAGTCCATCA TCGTAAGTGG AGAGTCTGGG GCAGGAAAAA CAGTCTCAGC TAAGTATGCC 
ATGCGATACT TTGCAACTGT GAGTGGTTCT GCCAGTGAGG CCAATGTGGA GGAAAAGGTC 
TTGGCCTCCA ACCCCATCAT GGAGTCCATT GGAAATGCTA AAACAACCAG GAATGATAAT 
AGCAGCCGTT TTGGGAAGTA TATTGAGATT GGTTTTGATA AGAGATATCG AATCATTGGT 
GCCAATATGA GAACTTATCT TTTAGAGAAA TCCAGAGTGG TATTCCAGGC AGAAGAGGAG 
AGAAACTATC ATATCTTCTA TCAGCTTTGT GCCTCAGCAA AGTTACCTGA ATTTAAAATG 
CTACGATTAG GAAATGCAGA TAACTTTAAT TACACAAAAC AAGGAGGCAG TCCTGTGATT 
GAAGGAGTGG ATGATGCAAA GGAGATGGCA CATACTAGGC AGGCCTGCAC TTTGCTAGGA 
ATTAGTGAAT CTCATCAAAT GGGAATTTTC CGAATACTTG CTGGCATCCT TCACTTAGGC 
AATGTTGGAT TTACATCCCG AGATGCAGAC AGCTGCACAA TACCTCCCAA GCATGAACCT 
CTCTGCATCT TCTGTGAACT CATGGGTGTG GACTATGAGG AGATGTGTCA CTGGCTCTGC 
CATCGGAAAC TGGCTACTGC CACAGAGACA TACATCAAGC CCATCTCCAA GCTGCAGGCC 
ACGAATGCCC GCGATGCTTT GGCCAAGCAC ATCTATGCCA AGCTCTTTAA CTGGATTGTA 
GATAATGTCA ATCAGGCTCT CCATTCTGCT GTCAAACAGC ACTCTTTTAT TGGTGTGCTA 
GACATTTACG GATTTGAAAC ATTTGAGATA AATAGTTTTG AACAGTTTTG CATAAATTAT 
GCAAATGAAA AACTACAGCA ACAATTCAAT ATGCATGTCT TCAAATTGGA GCAAGAAGAA 
TATATGAAGG AACAAATTCC ATGGACACTC ATAGATTTTT ATGATAATCA GCCTTGTATT 
AATCTTATAG AATCAAAACT AGGCATTCTA GATTTACTGG ATGAGGAATG CAAGATGCCT 
AAAGGCACAG ATGACACCTG GGCCCAAAAA TTGTACAACA CACATTTGAA CAAATGTGCA 
CTCTTTGAAA AGCCTCGTCT ATCAAACAAA GCTTTCATCA TCCAACATTT TGCTGACAAA 
GTGGAATACC AGTGTGAAGG ATTTCTCGAA AAGAATAAAG ACACCGTTTT TGAAGAACAA 
ATTAAAGTTC TTAAATCAAG CAAGTTTAAG ATGCTACCAG AACTATTTCA AGATGATGAG 
AAGGCCATCA GTCCAACTTC AGCCACCTCC TCAGGGCGCA CACCCCTCAC ACGAACTCCT 
GCAAAGCCCA CCAAAGGCAG ACCAGGCCAA ATGGCCAAAG AGCACAAGAA AACAGTGGGG 
CATCAGTTCA GAAACTCCCT GCACCTGCTT ATGGAGACAC TCAATGCCAC TACCCCTCAC 
TATGTGCGCT GTATCAAGCC TAATGACTTC AAGTTCCCAT TCACGTTTGA TGAGAAGAGG 
GCAGTGCAGC AGCTGAGAGC ATGTGGTGTC CTGGAAACCA TCCGAATCAG TGCGGCCGGT 
TTCCCCTCAC GGTGGACTTA CCAAGAATTT TTCAGCCGCT ACCGTGTCCT AATGAAGCAG 
AAAGATGTGC TGAGTGACAG AAAGCAAACA TGCAAGAATG TGTTAGAGAA ACTGATACTG 
GACAAGGACA AATACCAGTT TGGTAAGACA AAGATCTTTT TCCGTGCCGG TCAAGTGGCC 
TATCTAGAAA AATTGAGAGC TGACAAACTG AGAGCTGCCT GCATCCGGAT CCAGAAGACC 
ATCCGAGGGT GGCTGCTGAG AAAGAAGTAC CTACGCATGC GGAAGGCAGC CATCACCATG 
CAGAGATACG TGCGGGGCTA CCAGGCCCGA TGCTATGCTA AGTTTCTGCG CAGAACCAAG 
GCAGCAACCA TCATTCAAAA GTACTGGCGC ATGTATGTGG TCCGCAGGAG GTACAAGATT 
AGACGAGCTG CCACTATCGT TCTTCAGTCT TACTTGCGAG GCTTCTTGGC CAGAAATAGG 
TATCGCAAGA TACTCCGTGA GCACAAAGCA GTCATCATTC AGAAGCGAGT CCGGGGCTGG 
CTGGCCCGCA CACACTACAA GAGGAGCATG CATGCCATCA TCTACCTTCA GTGCTGCTTC 
AGGCGGATGA TGGCCAAGCG TGAGCTAAAG AAGCTCAAAA TCGAGGCTCG CTCAGTGGAG 
CGCTATAAGA AGCTGCACAT CGGCATGGAG AACAAGATCA TGCAGCTGCA GCGCAAAGTT 
GATGAGCAGA ACAAAGACTA CAAATGCCTT GTGGAGAAAC TAACCAATCT GGAAGGAATA 
TACAACTCTG AGACTGAGAA ACTACGAAGT GACTTAGAAC GTCTTCAACT AAGTGAAGAG 
GAAGCGAAAG TTGCCACTGG GCGGGTCCTT AGTCTGCAGG AAGAAATTGC CAAGCTCCGG 
AAAGACCTGG AGCAAACTCG TTCAGAGAAA AAATGCATTG AGGAACATGC AGATCGATAC 
AAACAAGAAA CAGAGCAGCT GGTATCAAAT CTGAAGGAAG AAAATACTTT GCTGAAGCAA 
GAAAAAGAAG CCCTCAATCA CCGCATCGTG CAGCAGGCTA AGGAGATGAC AGAAACTATG 
GAGAAGAAGT TAGTAGAAGA AACGAAACAA CTGGAACTCG ACCTTAATGA TGAAAGGCTG 
AGATATCAGA ACCTTCTGAA TGAGTTCAGT CGCCTGGAAG AAAGATATGA TGACCTCAAG 
GAAGAGATGA CCCTTATGGT GCATGTGCCT AAGCCTGGAC ACAAGAGAAC AGACTCCACC 
CACAGCAGCA ACGAGTCTGA ATATATCTTT AGCTCTGAAA TTGCAGAAAT GGAAGACATT 
CCATCAAGGA CAGAGGAACC AAGTGAGAAG AAGGTACCTC TGGACATGTC ATTGTTCCTT 
AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG CAGGAGAAGC AGGTGATGCA GGATGAGCTG 
GACCGCAAGG AGGAGCAGGT GCTCCGCAGC AAGGCCAAGG AAGAAGAAAG ACCACAAATT 
AGAGGTGCAG AACTGGAATA TGAGTCACTC AAGCGTCAAG AACTAGAATC AGAAAACAAA 
AAACTGAAGA ATGAGCTAAA TGAGTTGCGC AAGGCCCTCA GTGAGAAAAG TGCCCCAGAG 
GTGACCGCCC CAGGTGCACC TGCCTACCGT GTCCTCATGG AGCAGCTGAC CTCTGTGAGC 
GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC CTCATCTTAA GGTCTCAACT GGTGAGCCAG 
AAAGAGGCCA TCCAACCCAA GAATACAATG ACAGATTCCA CAATACTTTT GGAAGATGTA 
CAAAAAATGA AAGATAAAGG TGAAATAGCA CAAGCATACA TTGGTTTGAA AGAAACAAAT 
AGGCTCCTGG AATCCCAGCT GCAGTCACAG AAGAGGAGCC ATGAGAATGA GGCCGAGGCC 
CTCCGTGGGG AGATCCAGAG CCTGAAGGAG GAGAACAACC GACAGCAGCA GCTGCTGGCC 
CAGAACCTGC AGCTGCCCCC AGAGGCCCGC ATTGAGGCCA GCCTGCAGCA CGAGATCACC 
CGGCTGACCA ACGAAAACTT GGATTTGATG GAACAACTTG AAAAACAGGA TAAGACGGTC 
CGTAAACTGA AAAAACAACT GAAAGTATTT GCCAAAAAAA TTGGCGAACT AGAAGTGGGC 
CAGATGGAGA ACATATCCCC AGGACAGATC ATTGATGAAC CCATCCGACC AGTCAACATT 
CCCAGGAAAG AAAAGGATTT CCAAGGGATG CTGGAATACA AGAAGGAGGA TGAGCAAAAA 
CTTGTTAAGA ACCTGATTCT GGAACTGAAG CCACGTGGTG TAGCAGTCAA TTTGATTCCA 
GGATTACCGG CATATATCCT GTTCATGTGT GTTCGACATG CTGACTACCT GAATGATGAT 
CAGAAAGTAA GGTCGTTGCT AACATCAACA ATTAACAGCA TCAAAAAAGT ATTGAAGAAA 
AGAGGTGATG ATTTTGAAAC CGTCTCCTTC TGGCTCTCTA ACACATGCCG ATTTTTGCAC 
TGCTTGAAAC AGTACAGTGG AGAAGAGGGC TTTATGAAGC ACAACACATC TCGCCAGAAT 
GAACACTGCC TCACCAATTT TGACCTGGCT GAGTATCGGC AGGTGCTGAG TGACTTGGCC 
ATTCAGATCT ACCAGCAGCT CGTGCGGGTG TTAGAGAACA TCCTTCAGCC AATGATTGTC 
TCAGGCATGC TGGAACATGA AACGATTCAG GGCGTGTCTG GGGTGAAGCC CACAGGGTTG 
AGAAAGCGAA CCTCCAGTAT CGCCGATGAG GGCACCTACA CACTGGACTC CATCCTCCGG 
CAGCTCAACT CCTTCCACTC GGTCATGTGT CAGCATGGCA TGGACCCTGA ACTGATCAAG 
CAGGTGGTCA AGCAGATGTT CTACATCATA GGGGCCATCA CCCTGAACAA CCTTCTCCTG 
CGGAAGGACA TGTGCTCCTG GAGTAAAGGC ATGCAGATCA GGTACAATGT CAGTCAACTG 
GAAGAATGGC TGCGTGACAA GAATCTGATG AATAGTGGGG CTAAAGAAAC CCTGGAACCT 
CTCATTCAGG CTGCTCAACT TTTGCAAGTG AAAAAGAAAA CAGATGATGA TGCAGAAGCC 
ATTTGTTCTA TGTGCAATGC TTTAACTACT GCCCAGATTG TGAAAGTGTT GAATTTGTAT 
ACTCCAGTTA ATGAGTTTGA AGAAAGAGTC TCTGTGTCGT TCATTCGTAC TATACAGATG 
CGTTTACGAG ACAGGAAAGA CTCTCCCCAG CTGCTCATGG ATGCTAAACA CATCTTTCCT 
GTCACCTTTC CTTTCAACCC ATCTTCCCTC GCACTAGAAA CCATCCAGAT TCCAGCCAGC 
CTCGGCCTGG GCTTCATTTC ACGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( NM_001142495.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135967.1 Homo sapiens myosin VA (MYO5A), transcript variant 2, mRNA.

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CloneID	OHu24827
Clone ID Related Accession (Same CDS sequence)	NM_001142495.1
Accession Version	NM_001142495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Y07759.1, AB290180.1, AC010674.11 and BU738571.1. Transcript Variant: This variant (2) lacks an in-frame exon in the CDS, resulting in a shorter isoform (2), as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB290180.1, SRR1660807.115604.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGT CGGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCAGAGGAA 
GTCTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT CCTCCTGCTT 
CACCTCGAGG AAGGAAAGGA TTTGGAATAC CATCTAGATC CAAAGACCAA GGAGCTGCCT 
CACTTACGAA ATCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCCCT CAGCTATCTT 
CATGAGCCTG CTGTGCTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACGTATTGTG GTATAGTCCT AGTAGCTATA AATCCCTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA CAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATC 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GCGATACTTT 
GCAACTGTGA GTGGTTCTGC CAGTGAGGCC AATGTGGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG CAGCCGTTTT 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATTGGTGC CAATATGAGA 
ACTTATCTTT TAGAGAAATC CAGAGTGGTA TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AGCTTTGTGC CTCAGCAAAG TTACCTGAAT TTAAAATGCT ACGATTAGGA 
AATGCAGATA ACTTTAATTA CACAAAACAA GGAGGCAGTC CTGTGATTGA AGGAGTGGAT 
GATGCAAAGG AGATGGCACA TACTAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAAATGG GAATTTTCCG AATACTTGCT GGCATCCTTC ACTTAGGCAA TGTTGGATTT 
ACATCCCGAG ATGCAGACAG CTGCACAATA CCTCCCAAGC ATGAACCTCT CTGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTATGAGGAG ATGTGTCACT GGCTCTGCCA TCGGAAACTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGC 
GATGCTTTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TAATGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAACAGCAC TCTTTTATTG GTGTGCTAGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTCTTC AAATTGGAGC AAGAAGAATA TATGAAGGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGTATTAA TCTTATAGAA 
TCAAAACTAG GCATTCTAGA TTTACTGGAT GAGGAATGCA AGATGCCTAA AGGCACAGAT 
GACACCTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT CTTTGAAAAG 
CCTCGTCTAT CAAACAAAGC TTTCATCATC CAACATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGAT TTCTCGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCAACTTCAG CCACCTCCTC AGGGCGCACA CCCCTCACAC GAACTCCTGC AAAGCCCACC 
AAAGGCAGAC CAGGCCAAAT GGCCAAAGAG CACAAGAAAA CAGTGGGGCA TCAGTTCAGA 
AACTCCCTGC ACCTGCTTAT GGAGACACTC AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ATGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATC CGAATCAGTG CGGCCGGTTT CCCCTCACGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAC CGTGTCCTAA TGAAGCAGAA AGATGTGCTG 
AGTGACAGAA AGCAAACATG CAAGAATGTG TTAGAGAAAC TGATACTGGA CAAGGACAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCCGGTC AAGTGGCCTA TCTAGAAAAA 
TTGAGAGCTG ACAAACTGAG AGCTGCCTGC ATCCGGATCC AGAAGACCAT CCGAGGGTGG 
CTGCTGAGAA AGAAGTACCT ACGCATGCGG AAGGCAGCCA TCACCATGCA GAGATACGTG 
CGGGGCTACC AGGCCCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCATC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACTATTGTTC TTCAGTCTTA CTTGCGAGGC TTCTTGGCCA GAAATAGGTA TCGCAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGCGAGTCC GGGGCTGGCT GGCCCGCACA 
CACTACAAGA GGAGCATGCA TGCCATCATC TACCTTCAGT GCTGCTTCAG GCGGATGATG 
GCCAAGCGTG AGCTAAAGAA GCTCAAAATC GAGGCTCGCT CAGTGGAGCG CTATAAGAAG 
CTGCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTTGT GGAGAAACTA ACCAATCTGG AAGGAATATA CAACTCTGAG 
ACTGAGAAAC TACGAAGTGA CTTAGAACGT CTTCAACTAA GTGAAGAGGA AGCGAAAGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCCA AGCTCCGGAA AGACCTGGAG 
CAAACTCGTT CAGAGAAAAA ATGCATTGAG GAACATGCAG ATCGATACAA ACAAGAAACA 
GAGCAGCTGG TATCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAAGA AAAAGAAGCC 
CTCAATCACC GCATCGTGCA GCAGGCTAAG GAGATGACAG AAACTATGGA GAAGAAGTTA 
GTAGAAGAAA CGAAACAACT GGAACTCGAC CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAAGAA AGATATGATG ACCTCAAGGA AGAGATGACC 
CTTATGGTGC ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCCACCCA CAGCAGCAAC 
GAGTCTGAAT ATATCTTTAG CTCTGAAATT GCAGAAATGG AAGACATTCC ATCAAGGACA 
GAGGAACCAA GTGAGAAGAA GGTACCTCTG GACATGTCAT TGTTCCTTAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCCAAGGAA GAAGAAAGAC CACAAATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAATCAG AAAACAAAAA ACTGAAGAAT 
GAGCTAAATG AGTTGCGCAA GGCCCTCAGT GAGAAAAGTG CCCCAGAGGT GACCGCCCCA 
GGTGCACCTG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGCGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCTTAAGG TCTCAACTGG TGAGCCAGAA AGAGGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TACTTTTGGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATACATTG GTTTGAAAGA AACAAATAGG 
CTCCTGGAAT CCCAGCTGCA GTCACAGAAG AGGAGCCATG AGAATGAGGC CGAGGCCCTC 
CGTGGGGAGA TCCAGAGCCT GAAGGAGGAG AACAACCGAC AGCAGCAGCT GCTGGCCCAG 
AACCTGCAGC TGCCCCCAGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACCCGG 
CTGACCAACG AAAACTTGGA TTTGATGGAA CAACTTGAAA AACAGGATAA GACGGTCCGT 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GCGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCCCCAGG ACAGATCATT GATGAACCCA TCCGACCAGT CAACATTCCC 
AGGAAAGAAA AGGATTTCCA AGGGATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTTAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGA 
TTACCGGCAT ATATCCTGTT CATGTGTGTT CGACATGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AACAGCATCA AAAAAGTATT GAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTCTGG CTCTCTAACA CATGCCGATT TTTGCACTGC 
TTGAAACAGT ACAGTGGAGA AGAGGGCTTT ATGAAGCACA ACACATCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CCTGGCTGAG TATCGGCAGG TGCTGAGTGA CTTGGCCATT 
CAGATCTACC AGCAGCTCGT GCGGGTGTTA GAGAACATCC TTCAGCCAAT GATTGTCTCA 
GGCATGCTGG AACATGAAAC GATTCAGGGC GTGTCTGGGG TGAAGCCCAC AGGGTTGAGA 
AAGCGAACCT CCAGTATCGC CGATGAGGGC ACCTACACAC TGGACTCCAT CCTCCGGCAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CATGGCATGG ACCCTGAACT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCATAGGG GCCATCACCC TGAACAACCT TCTCCTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTGGAA 
GAATGGCTGC GTGACAAGAA TCTGATGAAT AGTGGGGCTA AAGAAACCCT GGAACCTCTC 
ATTCAGGCTG CTCAACTTTT GCAAGTGAAA AAGAAAACAG ATGATGATGC AGAAGCCATT 
TGTTCTATGT GCAATGCTTT AACTACTGCC CAGATTGTGA AAGTGTTGAA TTTGTATACT 
CCAGTTAATG AGTTTGAAGA AAGAGTCTCT GTGTCGTTCA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCCCAGCTG CTCATGGATG CTAAACACAT CTTTCCTGTC 
ACCTTTCCTT TCAACCCATC TTCCCTCGCA CTAGAAACCA TCCAGATTCC AGCCAGCCTC 
GGCCTGGGCT TCATTTCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135967.1
CDS	245..5731
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	NM_001142495.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142495.1

ORF Insert Sequence:

ATGGCTGCGT CGGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCAGAGGAA 
GTCTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT CCTCCTGCTT 
CACCTCGAGG AAGGAAAGGA TTTGGAATAC CATCTAGATC CAAAGACCAA GGAGCTGCCT 
CACTTACGAA ATCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCCCT CAGCTATCTT 
CATGAGCCTG CTGTGCTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACGTATTGTG GTATAGTCCT AGTAGCTATA AATCCCTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA CAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATC 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GCGATACTTT 
GCAACTGTGA GTGGTTCTGC CAGTGAGGCC AATGTGGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG CAGCCGTTTT 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATTGGTGC CAATATGAGA 
ACTTATCTTT TAGAGAAATC CAGAGTGGTA TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AGCTTTGTGC CTCAGCAAAG TTACCTGAAT TTAAAATGCT ACGATTAGGA 
AATGCAGATA ACTTTAATTA CACAAAACAA GGAGGCAGTC CTGTGATTGA AGGAGTGGAT 
GATGCAAAGG AGATGGCACA TACTAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAAATGG GAATTTTCCG AATACTTGCT GGCATCCTTC ACTTAGGCAA TGTTGGATTT 
ACATCCCGAG ATGCAGACAG CTGCACAATA CCTCCCAAGC ATGAACCTCT CTGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTATGAGGAG ATGTGTCACT GGCTCTGCCA TCGGAAACTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGC 
GATGCTTTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TAATGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAACAGCAC TCTTTTATTG GTGTGCTAGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTCTTC AAATTGGAGC AAGAAGAATA TATGAAGGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGTATTAA TCTTATAGAA 
TCAAAACTAG GCATTCTAGA TTTACTGGAT GAGGAATGCA AGATGCCTAA AGGCACAGAT 
GACACCTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT CTTTGAAAAG 
CCTCGTCTAT CAAACAAAGC TTTCATCATC CAACATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGAT TTCTCGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCAACTTCAG CCACCTCCTC AGGGCGCACA CCCCTCACAC GAACTCCTGC AAAGCCCACC 
AAAGGCAGAC CAGGCCAAAT GGCCAAAGAG CACAAGAAAA CAGTGGGGCA TCAGTTCAGA 
AACTCCCTGC ACCTGCTTAT GGAGACACTC AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ATGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATC CGAATCAGTG CGGCCGGTTT CCCCTCACGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAC CGTGTCCTAA TGAAGCAGAA AGATGTGCTG 
AGTGACAGAA AGCAAACATG CAAGAATGTG TTAGAGAAAC TGATACTGGA CAAGGACAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCCGGTC AAGTGGCCTA TCTAGAAAAA 
TTGAGAGCTG ACAAACTGAG AGCTGCCTGC ATCCGGATCC AGAAGACCAT CCGAGGGTGG 
CTGCTGAGAA AGAAGTACCT ACGCATGCGG AAGGCAGCCA TCACCATGCA GAGATACGTG 
CGGGGCTACC AGGCCCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCATC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACTATTGTTC TTCAGTCTTA CTTGCGAGGC TTCTTGGCCA GAAATAGGTA TCGCAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGCGAGTCC GGGGCTGGCT GGCCCGCACA 
CACTACAAGA GGAGCATGCA TGCCATCATC TACCTTCAGT GCTGCTTCAG GCGGATGATG 
GCCAAGCGTG AGCTAAAGAA GCTCAAAATC GAGGCTCGCT CAGTGGAGCG CTATAAGAAG 
CTGCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTTGT GGAGAAACTA ACCAATCTGG AAGGAATATA CAACTCTGAG 
ACTGAGAAAC TACGAAGTGA CTTAGAACGT CTTCAACTAA GTGAAGAGGA AGCGAAAGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCCA AGCTCCGGAA AGACCTGGAG 
CAAACTCGTT CAGAGAAAAA ATGCATTGAG GAACATGCAG ATCGATACAA ACAAGAAACA 
GAGCAGCTGG TATCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAAGA AAAAGAAGCC 
CTCAATCACC GCATCGTGCA GCAGGCTAAG GAGATGACAG AAACTATGGA GAAGAAGTTA 
GTAGAAGAAA CGAAACAACT GGAACTCGAC CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAAGAA AGATATGATG ACCTCAAGGA AGAGATGACC 
CTTATGGTGC ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCCACCCA CAGCAGCAAC 
GAGTCTGAAT ATATCTTTAG CTCTGAAATT GCAGAAATGG AAGACATTCC ATCAAGGACA 
GAGGAACCAA GTGAGAAGAA GGTACCTCTG GACATGTCAT TGTTCCTTAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCCAAGGAA GAAGAAAGAC CACAAATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAATCAG AAAACAAAAA ACTGAAGAAT 
GAGCTAAATG AGTTGCGCAA GGCCCTCAGT GAGAAAAGTG CCCCAGAGGT GACCGCCCCA 
GGTGCACCTG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGCGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCTTAAGG TCTCAACTGG TGAGCCAGAA AGAGGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TACTTTTGGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATACATTG GTTTGAAAGA AACAAATAGG 
CTCCTGGAAT CCCAGCTGCA GTCACAGAAG AGGAGCCATG AGAATGAGGC CGAGGCCCTC 
CGTGGGGAGA TCCAGAGCCT GAAGGAGGAG AACAACCGAC AGCAGCAGCT GCTGGCCCAG 
AACCTGCAGC TGCCCCCAGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACCCGG 
CTGACCAACG AAAACTTGGA TTTGATGGAA CAACTTGAAA AACAGGATAA GACGGTCCGT 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GCGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCCCCAGG ACAGATCATT GATGAACCCA TCCGACCAGT CAACATTCCC 
AGGAAAGAAA AGGATTTCCA AGGGATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTTAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGA 
TTACCGGCAT ATATCCTGTT CATGTGTGTT CGACATGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AACAGCATCA AAAAAGTATT GAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTCTGG CTCTCTAACA CATGCCGATT TTTGCACTGC 
TTGAAACAGT ACAGTGGAGA AGAGGGCTTT ATGAAGCACA ACACATCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CCTGGCTGAG TATCGGCAGG TGCTGAGTGA CTTGGCCATT 
CAGATCTACC AGCAGCTCGT GCGGGTGTTA GAGAACATCC TTCAGCCAAT GATTGTCTCA 
GGCATGCTGG AACATGAAAC GATTCAGGGC GTGTCTGGGG TGAAGCCCAC AGGGTTGAGA 
AAGCGAACCT CCAGTATCGC CGATGAGGGC ACCTACACAC TGGACTCCAT CCTCCGGCAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CATGGCATGG ACCCTGAACT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATCATAGGG GCCATCACCC TGAACAACCT TCTCCTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTGGAA 
GAATGGCTGC GTGACAAGAA TCTGATGAAT AGTGGGGCTA AAGAAACCCT GGAACCTCTC 
ATTCAGGCTG CTCAACTTTT GCAAGTGAAA AAGAAAACAG ATGATGATGC AGAAGCCATT 
TGTTCTATGT GCAATGCTTT AACTACTGCC CAGATTGTGA AAGTGTTGAA TTTGTATACT 
CCAGTTAATG AGTTTGAAGA AAGAGTCTCT GTGTCGTTCA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCCCAGCTG CTCATGGATG CTAAACACAT CTTTCCTGTC 
ACCTTTCCTT TCAACCCATC TTCCCTCGCA CTAGAAACCA TCCAGATTCC AGCCAGCCTC 
GGCCTGGGCT TCATTTCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( NM_000259.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000250.3 Homo sapiens myosin VA (MYO5A), transcript variant 1, mRNA.

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CloneID	OHu24969
Clone ID Related Accession (Same CDS sequence)	NM_000259.3
Accession Version	NM_000259.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U90942.1, AK307600.1, AF090426.1, AC010674.11 and BU738571.1. This sequence is a reference standard in the RefSeqGene project. On Dec 5, 2008 this sequence version replaced NM_000259.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U90942.1, Y07759.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGT CGGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCAGAGGAA 
GTCTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT CCTCCTGCTT 
CACCTCGAGG AAGGAAAGGA TTTGGAATAC CATCTAGATC CAAAGACCAA GGAGCTGCCT 
CACTTACGAA ATCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCCCT CAGCTATCTT 
CATGAGCCTG CTGTGCTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACGTATTGTG GTATAGTCCT AGTAGCTATA AATCCCTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA CAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATC 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GCGATACTTT 
GCAACTGTGA GTGGTTCTGC CAGTGAGGCC AATGTGGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG CAGCCGTTTT 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATTGGTGC CAATATGAGA 
ACTTATCTTT TAGAGAAATC CAGAGTGGTA TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AGCTTTGTGC CTCAGCAAAG TTACCTGAAT TTAAAATGCT ACGATTAGGA 
AATGCAGATA ACTTTAATTA CACAAAACAA GGAGGCAGTC CTGTGATTGA AGGAGTGGAT 
GATGCAAAGG AGATGGCACA TACTAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAAATGG GAATTTTCCG AATACTTGCT GGCATCCTTC ACTTAGGCAA TGTTGGATTT 
ACATCCCGAG ATGCAGACAG CTGCACAATA CCTCCCAAGC ATGAACCTCT CTGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTATGAGGAG ATGTGTCACT GGCTCTGCCA TCGGAAACTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGC 
GATGCTTTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TAATGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAACAGCAC TCTTTTATTG GTGTGCTAGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTCTTC AAATTGGAGC AAGAAGAATA TATGAAGGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGTATTAA TCTTATAGAA 
TCAAAACTAG GCATTCTAGA TTTACTGGAT GAGGAATGCA AGATGCCTAA AGGCACAGAT 
GACACCTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT CTTTGAAAAG 
CCTCGTCTAT CAAACAAAGC TTTCATCATC CAACATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGAT TTCTCGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCAACTTCAG CCACCTCCTC AGGGCGCACA CCCCTCACAC GAACTCCTGC AAAGCCCACC 
AAAGGCAGAC CAGGCCAAAT GGCCAAAGAG CACAAGAAAA CAGTGGGGCA TCAGTTCAGA 
AACTCCCTGC ACCTGCTTAT GGAGACACTC AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ATGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATC CGAATCAGTG CGGCCGGTTT CCCCTCACGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAC CGTGTCCTAA TGAAGCAGAA AGATGTGCTG 
AGTGACAGAA AGCAAACATG CAAGAATGTG TTAGAGAAAC TGATACTGGA CAAGGACAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCCGGTC AAGTGGCCTA TCTAGAAAAA 
TTGAGAGCTG ACAAACTGAG AGCTGCCTGC ATCCGGATCC AGAAGACCAT CCGAGGGTGG 
CTGCTGAGAA AGAAGTACCT ACGCATGCGG AAGGCAGCCA TCACCATGCA GAGATACGTG 
CGGGGCTACC AGGCCCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCATC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACTATTGTTC TTCAGTCTTA CTTGCGAGGC TTCTTGGCCA GAAATAGGTA TCGCAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGCGAGTCC GGGGCTGGCT GGCCCGCACA 
CACTACAAGA GGAGCATGCA TGCCATCATC TACCTTCAGT GCTGCTTCAG GCGGATGATG 
GCCAAGCGTG AGCTAAAGAA GCTCAAAATC GAGGCTCGCT CAGTGGAGCG CTATAAGAAG 
CTGCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTTGT GGAGAAACTA ACCAATCTGG AAGGAATATA CAACTCTGAG 
ACTGAGAAAC TACGAAGTGA CTTAGAACGT CTTCAACTAA GTGAAGAGGA AGCGAAAGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCCA AGCTCCGGAA AGACCTGGAG 
CAAACTCGTT CAGAGAAAAA ATGCATTGAG GAACATGCAG ATCGATACAA ACAAGAAACA 
GAGCAGCTGG TATCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAAGA AAAAGAAGCC 
CTCAATCACC GCATCGTGCA GCAGGCTAAG GAGATGACAG AAACTATGGA GAAGAAGTTA 
GTAGAAGAAA CGAAACAACT GGAACTCGAC CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAAGAA AGATATGATG ACCTCAAGGA AGAGATGACC 
CTTATGGTGC ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCCACCCA CAGCAGCAAC 
GAGTCTGAAT ATATCTTTAG CTCTGAAATT GCAGAAATGG AAGACATTCC ATCAAGGACA 
GAGGAACCAA GTGAGAAGAA GGTACCTCTG GACATGTCAT TGTTCCTTAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCCAAGGAA GAAGAAAGAC CACAAATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAATCAG AAAACAAAAA ACTGAAGAAT 
GAGCTAAATG AGTTGCGCAA GGCCCTCAGT GAGAAAAGTG CCCCAGAGGT GACCGCCCCA 
GGTGCACCTG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGCGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCTTAAGG TCTCAACTGG TGAGCCAGAA AGAGGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TACTTTTGGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATACATTG GTTTGAAAGA AACAAATAGA 
TCATCTGCTC TGGATTACCA TGAGTTGAAT GAGGATGGAG AGCTGTGGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAA TCCCAGCTGC AGTCACAGAA GAGGAGCCAT 
GAGAATGAGG CCGAGGCCCT CCGTGGGGAG ATCCAGAGCC TGAAGGAGGA GAACAACCGA 
CAGCAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACCCG GCTGACCAAC GAAAACTTGG ATTTGATGGA ACAACTTGAA 
AAACAGGATA AGACGGTCCG TAAACTGAAA AAACAACTGA AAGTATTTGC CAAAAAAATT 
GGCGAACTAG AAGTGGGCCA GATGGAGAAC ATATCCCCAG GACAGATCAT TGATGAACCC 
ATCCGACCAG TCAACATTCC CAGGAAAGAA AAGGATTTCC AAGGGATGCT GGAATACAAG 
AAGGAGGATG AGCAAAAACT TGTTAAGAAC CTGATTCTGG AACTGAAGCC ACGTGGTGTA 
GCAGTCAATT TGATTCCAGG ATTACCGGCA TATATCCTGT TCATGTGTGT TCGACATGCT 
GACTACCTGA ATGATGATCA GAAAGTAAGG TCGTTGCTAA CATCAACAAT TAACAGCATC 
AAAAAAGTAT TGAAGAAAAG AGGTGATGAT TTTGAAACCG TCTCCTTCTG GCTCTCTAAC 
ACATGCCGAT TTTTGCACTG CTTGAAACAG TACAGTGGAG AAGAGGGCTT TATGAAGCAC 
AACACATCTC GCCAGAATGA ACACTGCCTC ACCAATTTTG ACCTGGCTGA GTATCGGCAG 
GTGCTGAGTG ACTTGGCCAT TCAGATCTAC CAGCAGCTCG TGCGGGTGTT AGAGAACATC 
CTTCAGCCAA TGATTGTCTC AGGCATGCTG GAACATGAAA CGATTCAGGG CGTGTCTGGG 
GTGAAGCCCA CAGGGTTGAG AAAGCGAACC TCCAGTATCG CCGATGAGGG CACCTACACA 
CTGGACTCCA TCCTCCGGCA GCTCAACTCC TTCCACTCGG TCATGTGTCA GCATGGCATG 
GACCCTGAAC TGATCAAGCA GGTGGTCAAG CAGATGTTCT ACATCATAGG GGCCATCACC 
CTGAACAACC TTCTCCTGCG GAAGGACATG TGCTCCTGGA GTAAAGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTGGA AGAATGGCTG CGTGACAAGA ATCTGATGAA TAGTGGGGCT 
AAAGAAACCC TGGAACCTCT CATTCAGGCT GCTCAACTTT TGCAAGTGAA AAAGAAAACA 
GATGATGATG CAGAAGCCAT TTGTTCTATG TGCAATGCTT TAACTACTGC CCAGATTGTG 
AAAGTGTTGA ATTTGTATAC TCCAGTTAAT GAGTTTGAAG AAAGAGTCTC TGTGTCGTTC 
ATTCGTACTA TACAGATGCG TTTACGAGAC AGGAAAGACT CTCCCCAGCT GCTCATGGAT 
GCTAAACACA TCTTTCCTGT CACCTTTCCT TTCAACCCAT CTTCCCTCGC ACTAGAAACC 
ATCCAGATTC CAGCCAGCCT CGGCCTGGGC TTCATTTCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000250.3
CDS	245..5812
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	NM_000259.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000259.3

ORF Insert Sequence:

ATGGCTGCGT CGGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCAGAGGAA 
GTCTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT CCTCCTGCTT 
CACCTCGAGG AAGGAAAGGA TTTGGAATAC CATCTAGATC CAAAGACCAA GGAGCTGCCT 
CACTTACGAA ATCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCCCT CAGCTATCTT 
CATGAGCCTG CTGTGCTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACGTATTGTG GTATAGTCCT AGTAGCTATA AATCCCTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA CAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATC 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GCGATACTTT 
GCAACTGTGA GTGGTTCTGC CAGTGAGGCC AATGTGGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG CAGCCGTTTT 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATTGGTGC CAATATGAGA 
ACTTATCTTT TAGAGAAATC CAGAGTGGTA TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AGCTTTGTGC CTCAGCAAAG TTACCTGAAT TTAAAATGCT ACGATTAGGA 
AATGCAGATA ACTTTAATTA CACAAAACAA GGAGGCAGTC CTGTGATTGA AGGAGTGGAT 
GATGCAAAGG AGATGGCACA TACTAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAAATGG GAATTTTCCG AATACTTGCT GGCATCCTTC ACTTAGGCAA TGTTGGATTT 
ACATCCCGAG ATGCAGACAG CTGCACAATA CCTCCCAAGC ATGAACCTCT CTGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTATGAGGAG ATGTGTCACT GGCTCTGCCA TCGGAAACTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGC 
GATGCTTTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TAATGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAACAGCAC TCTTTTATTG GTGTGCTAGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTCTTC AAATTGGAGC AAGAAGAATA TATGAAGGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGTATTAA TCTTATAGAA 
TCAAAACTAG GCATTCTAGA TTTACTGGAT GAGGAATGCA AGATGCCTAA AGGCACAGAT 
GACACCTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT CTTTGAAAAG 
CCTCGTCTAT CAAACAAAGC TTTCATCATC CAACATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGAT TTCTCGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCAACTTCAG CCACCTCCTC AGGGCGCACA CCCCTCACAC GAACTCCTGC AAAGCCCACC 
AAAGGCAGAC CAGGCCAAAT GGCCAAAGAG CACAAGAAAA CAGTGGGGCA TCAGTTCAGA 
AACTCCCTGC ACCTGCTTAT GGAGACACTC AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ATGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATC CGAATCAGTG CGGCCGGTTT CCCCTCACGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAC CGTGTCCTAA TGAAGCAGAA AGATGTGCTG 
AGTGACAGAA AGCAAACATG CAAGAATGTG TTAGAGAAAC TGATACTGGA CAAGGACAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCCGGTC AAGTGGCCTA TCTAGAAAAA 
TTGAGAGCTG ACAAACTGAG AGCTGCCTGC ATCCGGATCC AGAAGACCAT CCGAGGGTGG 
CTGCTGAGAA AGAAGTACCT ACGCATGCGG AAGGCAGCCA TCACCATGCA GAGATACGTG 
CGGGGCTACC AGGCCCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCATC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACTATTGTTC TTCAGTCTTA CTTGCGAGGC TTCTTGGCCA GAAATAGGTA TCGCAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGCGAGTCC GGGGCTGGCT GGCCCGCACA 
CACTACAAGA GGAGCATGCA TGCCATCATC TACCTTCAGT GCTGCTTCAG GCGGATGATG 
GCCAAGCGTG AGCTAAAGAA GCTCAAAATC GAGGCTCGCT CAGTGGAGCG CTATAAGAAG 
CTGCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTTGT GGAGAAACTA ACCAATCTGG AAGGAATATA CAACTCTGAG 
ACTGAGAAAC TACGAAGTGA CTTAGAACGT CTTCAACTAA GTGAAGAGGA AGCGAAAGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCCA AGCTCCGGAA AGACCTGGAG 
CAAACTCGTT CAGAGAAAAA ATGCATTGAG GAACATGCAG ATCGATACAA ACAAGAAACA 
GAGCAGCTGG TATCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAAGA AAAAGAAGCC 
CTCAATCACC GCATCGTGCA GCAGGCTAAG GAGATGACAG AAACTATGGA GAAGAAGTTA 
GTAGAAGAAA CGAAACAACT GGAACTCGAC CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAAGAA AGATATGATG ACCTCAAGGA AGAGATGACC 
CTTATGGTGC ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCCACCCA CAGCAGCAAC 
GAGTCTGAAT ATATCTTTAG CTCTGAAATT GCAGAAATGG AAGACATTCC ATCAAGGACA 
GAGGAACCAA GTGAGAAGAA GGTACCTCTG GACATGTCAT TGTTCCTTAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCCAAGGAA GAAGAAAGAC CACAAATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAATCAG AAAACAAAAA ACTGAAGAAT 
GAGCTAAATG AGTTGCGCAA GGCCCTCAGT GAGAAAAGTG CCCCAGAGGT GACCGCCCCA 
GGTGCACCTG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGCGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCTTAAGG TCTCAACTGG TGAGCCAGAA AGAGGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TACTTTTGGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATACATTG GTTTGAAAGA AACAAATAGA 
TCATCTGCTC TGGATTACCA TGAGTTGAAT GAGGATGGAG AGCTGTGGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAA TCCCAGCTGC AGTCACAGAA GAGGAGCCAT 
GAGAATGAGG CCGAGGCCCT CCGTGGGGAG ATCCAGAGCC TGAAGGAGGA GAACAACCGA 
CAGCAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACCCG GCTGACCAAC GAAAACTTGG ATTTGATGGA ACAACTTGAA 
AAACAGGATA AGACGGTCCG TAAACTGAAA AAACAACTGA AAGTATTTGC CAAAAAAATT 
GGCGAACTAG AAGTGGGCCA GATGGAGAAC ATATCCCCAG GACAGATCAT TGATGAACCC 
ATCCGACCAG TCAACATTCC CAGGAAAGAA AAGGATTTCC AAGGGATGCT GGAATACAAG 
AAGGAGGATG AGCAAAAACT TGTTAAGAAC CTGATTCTGG AACTGAAGCC ACGTGGTGTA 
GCAGTCAATT TGATTCCAGG ATTACCGGCA TATATCCTGT TCATGTGTGT TCGACATGCT 
GACTACCTGA ATGATGATCA GAAAGTAAGG TCGTTGCTAA CATCAACAAT TAACAGCATC 
AAAAAAGTAT TGAAGAAAAG AGGTGATGAT TTTGAAACCG TCTCCTTCTG GCTCTCTAAC 
ACATGCCGAT TTTTGCACTG CTTGAAACAG TACAGTGGAG AAGAGGGCTT TATGAAGCAC 
AACACATCTC GCCAGAATGA ACACTGCCTC ACCAATTTTG ACCTGGCTGA GTATCGGCAG 
GTGCTGAGTG ACTTGGCCAT TCAGATCTAC CAGCAGCTCG TGCGGGTGTT AGAGAACATC 
CTTCAGCCAA TGATTGTCTC AGGCATGCTG GAACATGAAA CGATTCAGGG CGTGTCTGGG 
GTGAAGCCCA CAGGGTTGAG AAAGCGAACC TCCAGTATCG CCGATGAGGG CACCTACACA 
CTGGACTCCA TCCTCCGGCA GCTCAACTCC TTCCACTCGG TCATGTGTCA GCATGGCATG 
GACCCTGAAC TGATCAAGCA GGTGGTCAAG CAGATGTTCT ACATCATAGG GGCCATCACC 
CTGAACAACC TTCTCCTGCG GAAGGACATG TGCTCCTGGA GTAAAGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTGGA AGAATGGCTG CGTGACAAGA ATCTGATGAA TAGTGGGGCT 
AAAGAAACCC TGGAACCTCT CATTCAGGCT GCTCAACTTT TGCAAGTGAA AAAGAAAACA 
GATGATGATG CAGAAGCCAT TTGTTCTATG TGCAATGCTT TAACTACTGC CCAGATTGTG 
AAAGTGTTGA ATTTGTATAC TCCAGTTAAT GAGTTTGAAG AAAGAGTCTC TGTGTCGTTC 
ATTCGTACTA TACAGATGCG TTTACGAGAC AGGAAAGACT CTCCCCAGCT GCTCATGGAT 
GCTAAACACA TCTTTCCTGT CACCTTTCCT TTCAACCCAT CTTCCCTCGC ACTAGAAACC 
ATCCAGATTC CAGCCAGCCT CGGCCTGGGC TTCATTTCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_005254397.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254454.1 Homo sapiens myosin VA (MYO5A), transcript variant X3, mRNA.

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CloneID	OHu48319
Clone ID Related Accession (Same CDS sequence)	XM_005254397.3 , XM_005254397.4
Accession Version	XM_005254397.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGT CGGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCAGAGGAA 
GTCTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT CCTCCTGCTT 
CACCTCGAGG AAGGAAAGGA TTTGGAATAC CATCTAGATC CAAAGACCAA GGAGCTGCCT 
CACTTACGAA ATCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCCCT CAGCTATCTT 
CATGAGCCTG CTGTGCTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACGTATTGTG GTATAGTCCT AGTAGCTATA AATCCCTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA CAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATC 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GCGATACTTT 
GCAACTGTGA GTGGTTCTGC CAGTGAGGCC AATGTGGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG CAGCCGTTTT 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATTGGTGC CAATATGAGA 
ACTTATCTTT TAGAGAAATC CAGAGTGGTA TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AGCTTTGTGC CTCAGCAAAG TTACCTGAAT TTAAAATGCT ACGATTAGGA 
AATGCAGATA ACTTTAATTA CACAAAACAA GGAGGCAGTC CTGTGATTGA AGGAGTGGAT 
GATGCAAAGG AGATGGCACA TACTAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAAATGG GAATTTTCCG AATACTTGCT GGCATCCTTC ACTTAGGCAA TGTTGGATTT 
ACATCCCGAG ATGCAGACAG CTGCACAATA CCTCCCAAGC ATGAACCTCT CTGCATCTTC 
TGTGAACTCA TGGGTGTGGA CTATGAGGAG ATGTGTCACT GGCTCTGCCA TCGGAAACTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGC 
GATGCTTTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TAATGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAACAGCAC TCTTTTATTG GTGTGCTAGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTCTTC AAATTGGAGC AAGAAGAATA TATGAAGGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGTATTAA TCTTATAGAA 
TCAAAACTAG GCATTCTAGA TTTACTGGAT GAGGAATGCA AGATGCCTAA AGGCACAGAT 
GACACCTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT CTTTGAAAAG 
CCTCGTCTAT CAAACAAAGC TTTCATCATC CAACATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGAT TTCTCGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCAACTTCAG CCACCTCCTC AGGGCGCACA CCCCTCACAC GAACTCCTGC AAAGCCCACC 
AAAGGCAGAC CAGGCCAAAT GGCCAAAGAG CACAAGAAAA CAGTGGGGCA TCAGTTCAGA 
AACTCCCTGC ACCTGCTTAT GGAGACACTC AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ATGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATC CGAATCAGTG CGGCCGGTTT CCCCTCACGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAC CGTGTCCTAA TGAAGCAGAA AGATGTGCTG 
AGTGACAGAA AGCAAACATG CAAGAATGTG TTAGAGAAAC TGATACTGGA CAAGGACAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCCGGTC AAGTGGCCTA TCTAGAAAAA 
TTGAGAGCTG ACAAACTGAG AGCTGCCTGC ATCCGGATCC AGAAGACCAT CCGAGGGTGG 
CTGCTGAGAA AGAAGTACCT ACGCATGCGG AAGGCAGCCA TCACCATGCA GAGATACGTG 
CGGGGCTACC AGGCCCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCATC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACTATCGTTC TTCAGTCTTA CTTGCGAGGC TTCTTGGCCA GAAATAGGTA TCGCAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGCGAGTCC GGGGCTGGCT GGCCCGCACA 
CACTACAAGA GGAGCATGCA TGCCATCATC TACCTTCAGT GCTGCTTCAG GCGGATGATG 
GCCAAGCGTG AGCTAAAGAA GCTCAAAATC GAGGCTCGCT CAGTGGAGCG CTATAAGAAG 
CTGCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTTGT GGAGAAACTA ACCAATCTGG AAGGAATATA CAACTCTGAG 
ACTGAGAAAC TACGAAGTGA CTTAGAACGT CTTCAACTAA GTGAAGAGGA AGCGAAAGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCCA AGCTCCGGAA AGACCTGGAG 
CAAACTCGTT CAGAGAAAAA ATGCATTGAG GAACATGCAG ATCGATACAA ACAAGAAACA 
GAGCAGCTGG TATCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAAGA AAAAGAAGCC 
CTCAATCACC GCATCGTGCA GCAGGCTAAG GAGATGACAG AAACTATGGA GAAGAAGTTA 
GTAGAAGAAA CGAAACAACT GGAACTCGAC CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAAGAA AGATATGATG ACCTCAAGGA AGAGATGACC 
CTTATGGTGC ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCCACCCA CAGCAGCAAC 
GAGTCTGAAT ATATCTTTAG CTCTGAAATT GCAGAAATGG AAGACATTCC ATCAAGGACA 
GAGGAACCAA GTGAGAAGAA GGTACCTCTG GACATGTCAT TGTTCCTTAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCCAAGGAA GAAGAAAGAC CACAAATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAATCAG AAAACAAAAA ACTGAAGAAT 
GAGCTAAATG AGTTGCGCAA GGCCCTCAGT GAGAAAAGTG CCCCAGAGGT GACCGCCCCA 
GGTGCACCTG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGCGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCTTAAGG TCTCAACTGG TGAGCCAGAA AGAGGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TACTTTTGGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATACATTG GTTTGAAAGA AACAAATAGA 
TCATCTGCTC TGGATTACCA TGAGTTGAAT GAGGATGGAG AGCTGTGGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAA TCCCAGCTGC AGTCACAGAA GAGGAGCCAT 
GAGAATGAGG CCGAGGCCCT CCGTGGGGAG ATCCAGAGCC TGAAGGAGGA GAACAACCGA 
CAGCAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACCCG GCTGACCAAC GAAAACTTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCCCTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TTGAAAAACA GGATAAGACG GTCCGTAAAC TGAAAAAACA ACTGAAAGTA 
TTTGCCAAAA AAATTGGCGA ACTAGAAGTG GGCCAGATGG AGAACATATC CCCAGGACAG 
ATCATTGATG AACCCATCCG ACCAGTCAAC ATTCCCAGGA AAGAAAAGGA TTTCCAAGGG 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTTA AGAACCTGAT TCTGGAACTG 
AAGCCACGTG GTGTAGCAGT CAATTTGATT CCAGGATTAC CGGCATATAT CCTGTTCATG 
TGTGTTCGAC ATGCTGACTA CCTGAATGAT GATCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAACA GCATCAAAAA AGTATTGAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTCTGGCTCT CTAACACATG CCGATTTTTG CACTGCTTGA AACAGTACAG TGGAGAAGAG 
GGCTTTATGA AGCACAACAC ATCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACCTG 
GCTGAGTATC GGCAGGTGCT GAGTGACTTG GCCATTCAGA TCTACCAGCA GCTCGTGCGG 
GTGTTAGAGA ACATCCTTCA GCCAATGATT GTCTCAGGCA TGCTGGAACA TGAAACGATT 
CAGGGCGTGT CTGGGGTGAA GCCCACAGGG TTGAGAAAGC GAACCTCCAG TATCGCCGAT 
GAGGGCACCT ACACACTGGA CTCCATCCTC CGGCAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCATG GCATGGACCC TGAACTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATC 
ATAGGGGCCA TCACCCTGAA CAACCTTCTC CTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTGGAAGAAT GGCTGCGTGA CAAGAATCTG 
ATGAATAGTG GGGCTAAAGA AACCCTGGAA CCTCTCATTC AGGCTGCTCA ACTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGATGCAGAA GCCATTTGTT CTATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTGAAAGT GTTGAATTTG TATACTCCAG TTAATGAGTT TGAAGAAAGA 
GTCTCTGTGT CGTTCATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCC 
CAGCTGCTCA TGGATGCTAA ACACATCTTT CCTGTCACCT TTCCTTTCAA CCCATCTTCC 
CTCGCACTAG AAACCATCCA GATTCCAGCC AGCCTCGGCC TGGGCTTCAT TTCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254454.1
CDS	77..5719
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_005254397.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254397.3

ORF Insert Sequence:

ATGGCTGCGT CGGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCAGAGGAA 
GTCTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT CCTCCTGCTT 
CACCTCGAGG AAGGAAAGGA TTTGGAATAC CATCTAGATC CAAAGACCAA GGAGCTGCCT 
CACTTACGAA ATCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCCCT CAGCTATCTT 
CATGAGCCTG CTGTGCTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACGTATTGTG GTATAGTCCT AGTAGCTATA AATCCCTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA CAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATC 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GCGATACTTT 
GCAACTGTGA GTGGTTCTGC CAGTGAGGCC AATGTGGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG CAGCCGTTTT 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATTGGTGC CAATATGAGA 
ACTTATCTTT TAGAGAAATC CAGAGTGGTA TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AGCTTTGTGC CTCAGCAAAG TTACCTGAAT TTAAAATGCT ACGATTAGGA 
AATGCAGATA ACTTTAATTA CACAAAACAA GGAGGCAGTC CTGTGATTGA AGGAGTGGAT 
GATGCAAAGG AGATGGCACA TACTAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAAATGG GAATTTTCCG AATACTTGCT GGCATCCTTC ACTTAGGCAA TGTTGGATTT 
ACATCCCGAG ATGCAGACAG CTGCACAATA CCTCCCAAGC ATGAACCTCT CTGCATCTTC 
TGTGAACTCA TGGGTGTGGA CTATGAGGAG ATGTGTCACT GGCTCTGCCA TCGGAAACTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGC 
GATGCTTTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TAATGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAACAGCAC TCTTTTATTG GTGTGCTAGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTCTTC AAATTGGAGC AAGAAGAATA TATGAAGGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGTATTAA TCTTATAGAA 
TCAAAACTAG GCATTCTAGA TTTACTGGAT GAGGAATGCA AGATGCCTAA AGGCACAGAT 
GACACCTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT CTTTGAAAAG 
CCTCGTCTAT CAAACAAAGC TTTCATCATC CAACATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGAT TTCTCGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCAACTTCAG CCACCTCCTC AGGGCGCACA CCCCTCACAC GAACTCCTGC AAAGCCCACC 
AAAGGCAGAC CAGGCCAAAT GGCCAAAGAG CACAAGAAAA CAGTGGGGCA TCAGTTCAGA 
AACTCCCTGC ACCTGCTTAT GGAGACACTC AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ATGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATC CGAATCAGTG CGGCCGGTTT CCCCTCACGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAC CGTGTCCTAA TGAAGCAGAA AGATGTGCTG 
AGTGACAGAA AGCAAACATG CAAGAATGTG TTAGAGAAAC TGATACTGGA CAAGGACAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCCGGTC AAGTGGCCTA TCTAGAAAAA 
TTGAGAGCTG ACAAACTGAG AGCTGCCTGC ATCCGGATCC AGAAGACCAT CCGAGGGTGG 
CTGCTGAGAA AGAAGTACCT ACGCATGCGG AAGGCAGCCA TCACCATGCA GAGATACGTG 
CGGGGCTACC AGGCCCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCATC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACTATCGTTC TTCAGTCTTA CTTGCGAGGC TTCTTGGCCA GAAATAGGTA TCGCAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGCGAGTCC GGGGCTGGCT GGCCCGCACA 
CACTACAAGA GGAGCATGCA TGCCATCATC TACCTTCAGT GCTGCTTCAG GCGGATGATG 
GCCAAGCGTG AGCTAAAGAA GCTCAAAATC GAGGCTCGCT CAGTGGAGCG CTATAAGAAG 
CTGCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTTGT GGAGAAACTA ACCAATCTGG AAGGAATATA CAACTCTGAG 
ACTGAGAAAC TACGAAGTGA CTTAGAACGT CTTCAACTAA GTGAAGAGGA AGCGAAAGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCCA AGCTCCGGAA AGACCTGGAG 
CAAACTCGTT CAGAGAAAAA ATGCATTGAG GAACATGCAG ATCGATACAA ACAAGAAACA 
GAGCAGCTGG TATCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAAGA AAAAGAAGCC 
CTCAATCACC GCATCGTGCA GCAGGCTAAG GAGATGACAG AAACTATGGA GAAGAAGTTA 
GTAGAAGAAA CGAAACAACT GGAACTCGAC CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAAGAA AGATATGATG ACCTCAAGGA AGAGATGACC 
CTTATGGTGC ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCCACCCA CAGCAGCAAC 
GAGTCTGAAT ATATCTTTAG CTCTGAAATT GCAGAAATGG AAGACATTCC ATCAAGGACA 
GAGGAACCAA GTGAGAAGAA GGTACCTCTG GACATGTCAT TGTTCCTTAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCCAAGGAA GAAGAAAGAC CACAAATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAATCAG AAAACAAAAA ACTGAAGAAT 
GAGCTAAATG AGTTGCGCAA GGCCCTCAGT GAGAAAAGTG CCCCAGAGGT GACCGCCCCA 
GGTGCACCTG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGCGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCTTAAGG TCTCAACTGG TGAGCCAGAA AGAGGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TACTTTTGGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATACATTG GTTTGAAAGA AACAAATAGA 
TCATCTGCTC TGGATTACCA TGAGTTGAAT GAGGATGGAG AGCTGTGGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAA TCCCAGCTGC AGTCACAGAA GAGGAGCCAT 
GAGAATGAGG CCGAGGCCCT CCGTGGGGAG ATCCAGAGCC TGAAGGAGGA GAACAACCGA 
CAGCAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACCCG GCTGACCAAC GAAAACTTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCCCTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TTGAAAAACA GGATAAGACG GTCCGTAAAC TGAAAAAACA ACTGAAAGTA 
TTTGCCAAAA AAATTGGCGA ACTAGAAGTG GGCCAGATGG AGAACATATC CCCAGGACAG 
ATCATTGATG AACCCATCCG ACCAGTCAAC ATTCCCAGGA AAGAAAAGGA TTTCCAAGGG 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTTA AGAACCTGAT TCTGGAACTG 
AAGCCACGTG GTGTAGCAGT CAATTTGATT CCAGGATTAC CGGCATATAT CCTGTTCATG 
TGTGTTCGAC ATGCTGACTA CCTGAATGAT GATCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAACA GCATCAAAAA AGTATTGAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTCTGGCTCT CTAACACATG CCGATTTTTG CACTGCTTGA AACAGTACAG TGGAGAAGAG 
GGCTTTATGA AGCACAACAC ATCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACCTG 
GCTGAGTATC GGCAGGTGCT GAGTGACTTG GCCATTCAGA TCTACCAGCA GCTCGTGCGG 
GTGTTAGAGA ACATCCTTCA GCCAATGATT GTCTCAGGCA TGCTGGAACA TGAAACGATT 
CAGGGCGTGT CTGGGGTGAA GCCCACAGGG TTGAGAAAGC GAACCTCCAG TATCGCCGAT 
GAGGGCACCT ACACACTGGA CTCCATCCTC CGGCAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCATG GCATGGACCC TGAACTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATC 
ATAGGGGCCA TCACCCTGAA CAACCTTCTC CTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTGGAAGAAT GGCTGCGTGA CAAGAATCTG 
ATGAATAGTG GGGCTAAAGA AACCCTGGAA CCTCTCATTC AGGCTGCTCA ACTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGATGCAGAA GCCATTTGTT CTATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTGAAAGT GTTGAATTTG TATACTCCAG TTAATGAGTT TGAAGAAAGA 
GTCTCTGTGT CGTTCATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCC 
CAGCTGCTCA TGGATGCTAA ACACATCTTT CCTGTCACCT TTCCTTTCAA CCCATCTTCC 
CTCGCACTAG AAACCATCCA GATTCCAGCC AGCCTCGGCC TGGGCTTCAT TTCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_005254397.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254454.1 Homo sapiens myosin VA (MYO5A), transcript variant X3, mRNA.

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CloneID	OHu48319
Clone ID Related Accession (Same CDS sequence)	XM_005254397.3 , XM_005254397.4
Accession Version	XM_005254397.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005254397.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGT CGGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCAGAGGAA 
GTCTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT CCTCCTGCTT 
CACCTCGAGG AAGGAAAGGA TTTGGAATAC CATCTAGATC CAAAGACCAA GGAGCTGCCT 
CACTTACGAA ATCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCCCT CAGCTATCTT 
CATGAGCCTG CTGTGCTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACGTATTGTG GTATAGTCCT AGTAGCTATA AATCCCTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA CAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATC 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GCGATACTTT 
GCAACTGTGA GTGGTTCTGC CAGTGAGGCC AATGTGGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG CAGCCGTTTT 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATTGGTGC CAATATGAGA 
ACTTATCTTT TAGAGAAATC CAGAGTGGTA TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AGCTTTGTGC CTCAGCAAAG TTACCTGAAT TTAAAATGCT ACGATTAGGA 
AATGCAGATA ACTTTAATTA CACAAAACAA GGAGGCAGTC CTGTGATTGA AGGAGTGGAT 
GATGCAAAGG AGATGGCACA TACTAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAAATGG GAATTTTCCG AATACTTGCT GGCATCCTTC ACTTAGGCAA TGTTGGATTT 
ACATCCCGAG ATGCAGACAG CTGCACAATA CCTCCCAAGC ATGAACCTCT CTGCATCTTC 
TGTGAACTCA TGGGTGTGGA CTATGAGGAG ATGTGTCACT GGCTCTGCCA TCGGAAACTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGC 
GATGCTTTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TAATGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAACAGCAC TCTTTTATTG GTGTGCTAGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTCTTC AAATTGGAGC AAGAAGAATA TATGAAGGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGTATTAA TCTTATAGAA 
TCAAAACTAG GCATTCTAGA TTTACTGGAT GAGGAATGCA AGATGCCTAA AGGCACAGAT 
GACACCTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT CTTTGAAAAG 
CCTCGTCTAT CAAACAAAGC TTTCATCATC CAACATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGAT TTCTCGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCAACTTCAG CCACCTCCTC AGGGCGCACA CCCCTCACAC GAACTCCTGC AAAGCCCACC 
AAAGGCAGAC CAGGCCAAAT GGCCAAAGAG CACAAGAAAA CAGTGGGGCA TCAGTTCAGA 
AACTCCCTGC ACCTGCTTAT GGAGACACTC AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ATGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATC CGAATCAGTG CGGCCGGTTT CCCCTCACGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAC CGTGTCCTAA TGAAGCAGAA AGATGTGCTG 
AGTGACAGAA AGCAAACATG CAAGAATGTG TTAGAGAAAC TGATACTGGA CAAGGACAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCCGGTC AAGTGGCCTA TCTAGAAAAA 
TTGAGAGCTG ACAAACTGAG AGCTGCCTGC ATCCGGATCC AGAAGACCAT CCGAGGGTGG 
CTGCTGAGAA AGAAGTACCT ACGCATGCGG AAGGCAGCCA TCACCATGCA GAGATACGTG 
CGGGGCTACC AGGCCCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCATC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACTATCGTTC TTCAGTCTTA CTTGCGAGGC TTCTTGGCCA GAAATAGGTA TCGCAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGCGAGTCC GGGGCTGGCT GGCCCGCACA 
CACTACAAGA GGAGCATGCA TGCCATCATC TACCTTCAGT GCTGCTTCAG GCGGATGATG 
GCCAAGCGTG AGCTAAAGAA GCTCAAAATC GAGGCTCGCT CAGTGGAGCG CTATAAGAAG 
CTGCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTTGT GGAGAAACTA ACCAATCTGG AAGGAATATA CAACTCTGAG 
ACTGAGAAAC TACGAAGTGA CTTAGAACGT CTTCAACTAA GTGAAGAGGA AGCGAAAGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCCA AGCTCCGGAA AGACCTGGAG 
CAAACTCGTT CAGAGAAAAA ATGCATTGAG GAACATGCAG ATCGATACAA ACAAGAAACA 
GAGCAGCTGG TATCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAAGA AAAAGAAGCC 
CTCAATCACC GCATCGTGCA GCAGGCTAAG GAGATGACAG AAACTATGGA GAAGAAGTTA 
GTAGAAGAAA CGAAACAACT GGAACTCGAC CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAAGAA AGATATGATG ACCTCAAGGA AGAGATGACC 
CTTATGGTGC ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCCACCCA CAGCAGCAAC 
GAGTCTGAAT ATATCTTTAG CTCTGAAATT GCAGAAATGG AAGACATTCC ATCAAGGACA 
GAGGAACCAA GTGAGAAGAA GGTACCTCTG GACATGTCAT TGTTCCTTAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCCAAGGAA GAAGAAAGAC CACAAATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAATCAG AAAACAAAAA ACTGAAGAAT 
GAGCTAAATG AGTTGCGCAA GGCCCTCAGT GAGAAAAGTG CCCCAGAGGT GACCGCCCCA 
GGTGCACCTG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGCGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCTTAAGG TCTCAACTGG TGAGCCAGAA AGAGGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TACTTTTGGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATACATTG GTTTGAAAGA AACAAATAGA 
TCATCTGCTC TGGATTACCA TGAGTTGAAT GAGGATGGAG AGCTGTGGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAA TCCCAGCTGC AGTCACAGAA GAGGAGCCAT 
GAGAATGAGG CCGAGGCCCT CCGTGGGGAG ATCCAGAGCC TGAAGGAGGA GAACAACCGA 
CAGCAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACCCG GCTGACCAAC GAAAACTTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCCCTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TTGAAAAACA GGATAAGACG GTCCGTAAAC TGAAAAAACA ACTGAAAGTA 
TTTGCCAAAA AAATTGGCGA ACTAGAAGTG GGCCAGATGG AGAACATATC CCCAGGACAG 
ATCATTGATG AACCCATCCG ACCAGTCAAC ATTCCCAGGA AAGAAAAGGA TTTCCAAGGG 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTTA AGAACCTGAT TCTGGAACTG 
AAGCCACGTG GTGTAGCAGT CAATTTGATT CCAGGATTAC CGGCATATAT CCTGTTCATG 
TGTGTTCGAC ATGCTGACTA CCTGAATGAT GATCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAACA GCATCAAAAA AGTATTGAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTCTGGCTCT CTAACACATG CCGATTTTTG CACTGCTTGA AACAGTACAG TGGAGAAGAG 
GGCTTTATGA AGCACAACAC ATCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACCTG 
GCTGAGTATC GGCAGGTGCT GAGTGACTTG GCCATTCAGA TCTACCAGCA GCTCGTGCGG 
GTGTTAGAGA ACATCCTTCA GCCAATGATT GTCTCAGGCA TGCTGGAACA TGAAACGATT 
CAGGGCGTGT CTGGGGTGAA GCCCACAGGG TTGAGAAAGC GAACCTCCAG TATCGCCGAT 
GAGGGCACCT ACACACTGGA CTCCATCCTC CGGCAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCATG GCATGGACCC TGAACTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATC 
ATAGGGGCCA TCACCCTGAA CAACCTTCTC CTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTGGAAGAAT GGCTGCGTGA CAAGAATCTG 
ATGAATAGTG GGGCTAAAGA AACCCTGGAA CCTCTCATTC AGGCTGCTCA ACTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGATGCAGAA GCCATTTGTT CTATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTGAAAGT GTTGAATTTG TATACTCCAG TTAATGAGTT TGAAGAAAGA 
GTCTCTGTGT CGTTCATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCC 
CAGCTGCTCA TGGATGCTAA ACACATCTTT CCTGTCACCT TTCCTTTCAA CCCATCTTCC 
CTCGCACTAG AAACCATCCA GATTCCAGCC AGCCTCGGCC TGGGCTTCAT TTCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254454.1
CDS	77..5719
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_005254397.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VA (MYO5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254397.4

ORF Insert Sequence:

ATGGCTGCGT CGGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCAGAGGAA 
GTCTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT CCTCCTGCTT 
CACCTCGAGG AAGGAAAGGA TTTGGAATAC CATCTAGATC CAAAGACCAA GGAGCTGCCT 
CACTTACGAA ATCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCCCT CAGCTATCTT 
CATGAGCCTG CTGTGCTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACGTATTGTG GTATAGTCCT AGTAGCTATA AATCCCTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA CAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATC 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTCTCAGCTA AGTATGCCAT GCGATACTTT 
GCAACTGTGA GTGGTTCTGC CAGTGAGGCC AATGTGGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCCATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG CAGCCGTTTT 
GGGAAGTATA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATTGGTGC CAATATGAGA 
ACTTATCTTT TAGAGAAATC CAGAGTGGTA TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AGCTTTGTGC CTCAGCAAAG TTACCTGAAT TTAAAATGCT ACGATTAGGA 
AATGCAGATA ACTTTAATTA CACAAAACAA GGAGGCAGTC CTGTGATTGA AGGAGTGGAT 
GATGCAAAGG AGATGGCACA TACTAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAAATGG GAATTTTCCG AATACTTGCT GGCATCCTTC ACTTAGGCAA TGTTGGATTT 
ACATCCCGAG ATGCAGACAG CTGCACAATA CCTCCCAAGC ATGAACCTCT CTGCATCTTC 
TGTGAACTCA TGGGTGTGGA CTATGAGGAG ATGTGTCACT GGCTCTGCCA TCGGAAACTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGC 
GATGCTTTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TAATGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAACAGCAC TCTTTTATTG GTGTGCTAGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCATGTCTTC AAATTGGAGC AAGAAGAATA TATGAAGGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGTATTAA TCTTATAGAA 
TCAAAACTAG GCATTCTAGA TTTACTGGAT GAGGAATGCA AGATGCCTAA AGGCACAGAT 
GACACCTGGG CCCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT CTTTGAAAAG 
CCTCGTCTAT CAAACAAAGC TTTCATCATC CAACATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGAT TTCTCGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCAACTTCAG CCACCTCCTC AGGGCGCACA CCCCTCACAC GAACTCCTGC AAAGCCCACC 
AAAGGCAGAC CAGGCCAAAT GGCCAAAGAG CACAAGAAAA CAGTGGGGCA TCAGTTCAGA 
AACTCCCTGC ACCTGCTTAT GGAGACACTC AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ATGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GTGGTGTCCT GGAAACCATC CGAATCAGTG CGGCCGGTTT CCCCTCACGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAC CGTGTCCTAA TGAAGCAGAA AGATGTGCTG 
AGTGACAGAA AGCAAACATG CAAGAATGTG TTAGAGAAAC TGATACTGGA CAAGGACAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCCGGTC AAGTGGCCTA TCTAGAAAAA 
TTGAGAGCTG ACAAACTGAG AGCTGCCTGC ATCCGGATCC AGAAGACCAT CCGAGGGTGG 
CTGCTGAGAA AGAAGTACCT ACGCATGCGG AAGGCAGCCA TCACCATGCA GAGATACGTG 
CGGGGCTACC AGGCCCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCATC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACTATCGTTC TTCAGTCTTA CTTGCGAGGC TTCTTGGCCA GAAATAGGTA TCGCAAGATA 
CTCCGTGAGC ACAAAGCAGT CATCATTCAG AAGCGAGTCC GGGGCTGGCT GGCCCGCACA 
CACTACAAGA GGAGCATGCA TGCCATCATC TACCTTCAGT GCTGCTTCAG GCGGATGATG 
GCCAAGCGTG AGCTAAAGAA GCTCAAAATC GAGGCTCGCT CAGTGGAGCG CTATAAGAAG 
CTGCACATCG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTTGT GGAGAAACTA ACCAATCTGG AAGGAATATA CAACTCTGAG 
ACTGAGAAAC TACGAAGTGA CTTAGAACGT CTTCAACTAA GTGAAGAGGA AGCGAAAGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCCA AGCTCCGGAA AGACCTGGAG 
CAAACTCGTT CAGAGAAAAA ATGCATTGAG GAACATGCAG ATCGATACAA ACAAGAAACA 
GAGCAGCTGG TATCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAAGA AAAAGAAGCC 
CTCAATCACC GCATCGTGCA GCAGGCTAAG GAGATGACAG AAACTATGGA GAAGAAGTTA 
GTAGAAGAAA CGAAACAACT GGAACTCGAC CTTAATGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAAGAA AGATATGATG ACCTCAAGGA AGAGATGACC 
CTTATGGTGC ATGTGCCTAA GCCTGGACAC AAGAGAACAG ACTCCACCCA CAGCAGCAAC 
GAGTCTGAAT ATATCTTTAG CTCTGAAATT GCAGAAATGG AAGACATTCC ATCAAGGACA 
GAGGAACCAA GTGAGAAGAA GGTACCTCTG GACATGTCAT TGTTCCTTAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCCAAGGAA GAAGAAAGAC CACAAATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAATCAG AAAACAAAAA ACTGAAGAAT 
GAGCTAAATG AGTTGCGCAA GGCCCTCAGT GAGAAAAGTG CCCCAGAGGT GACCGCCCCA 
GGTGCACCTG CCTACCGTGT CCTCATGGAG CAGCTGACCT CTGTGAGCGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCTTAAGG TCTCAACTGG TGAGCCAGAA AGAGGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TACTTTTGGA AGATGTACAA 
AAAATGAAAG ATAAAGGTGA AATAGCACAA GCATACATTG GTTTGAAAGA AACAAATAGA 
TCATCTGCTC TGGATTACCA TGAGTTGAAT GAGGATGGAG AGCTGTGGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAA TCCCAGCTGC AGTCACAGAA GAGGAGCCAT 
GAGAATGAGG CCGAGGCCCT CCGTGGGGAG ATCCAGAGCC TGAAGGAGGA GAACAACCGA 
CAGCAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCAG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACCCG GCTGACCAAC GAAAACTTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCCCTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TTGAAAAACA GGATAAGACG GTCCGTAAAC TGAAAAAACA ACTGAAAGTA 
TTTGCCAAAA AAATTGGCGA ACTAGAAGTG GGCCAGATGG AGAACATATC CCCAGGACAG 
ATCATTGATG AACCCATCCG ACCAGTCAAC ATTCCCAGGA AAGAAAAGGA TTTCCAAGGG 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTTA AGAACCTGAT TCTGGAACTG 
AAGCCACGTG GTGTAGCAGT CAATTTGATT CCAGGATTAC CGGCATATAT CCTGTTCATG 
TGTGTTCGAC ATGCTGACTA CCTGAATGAT GATCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAACA GCATCAAAAA AGTATTGAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTCTGGCTCT CTAACACATG CCGATTTTTG CACTGCTTGA AACAGTACAG TGGAGAAGAG 
GGCTTTATGA AGCACAACAC ATCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACCTG 
GCTGAGTATC GGCAGGTGCT GAGTGACTTG GCCATTCAGA TCTACCAGCA GCTCGTGCGG 
GTGTTAGAGA ACATCCTTCA GCCAATGATT GTCTCAGGCA TGCTGGAACA TGAAACGATT 
CAGGGCGTGT CTGGGGTGAA GCCCACAGGG TTGAGAAAGC GAACCTCCAG TATCGCCGAT 
GAGGGCACCT ACACACTGGA CTCCATCCTC CGGCAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCATG GCATGGACCC TGAACTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATC 
ATAGGGGCCA TCACCCTGAA CAACCTTCTC CTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTGGAAGAAT GGCTGCGTGA CAAGAATCTG 
ATGAATAGTG GGGCTAAAGA AACCCTGGAA CCTCTCATTC AGGCTGCTCA ACTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGATGCAGAA GCCATTTGTT CTATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTGAAAGT GTTGAATTTG TATACTCCAG TTAATGAGTT TGAAGAAAGA 
GTCTCTGTGT CGTTCATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCC 
CAGCTGCTCA TGGATGCTAA ACACATCTTT CCTGTCACCT TTCCTTTCAA CCCATCTTCC 
CTCGCACTAG AAACCATCCA GATTCCAGCC AGCCTCGGCC TGGGCTTCAT TTCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MYO5A
Entrez Gene ID	4644
Full Name	myosin VA
Synonyms	GS1,MYH12,MYO5,MYR12
General protein information	Preferred Names myosin VA Names unconventional myosin-Va dilute myosin heavy chain, non-muscle myosin V myosin VA (heavy chain 12, myoxin) myosin, heavy polypeptide kinase myosin-12 myosin-Va myoxin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q21.2
Summary	This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008].
Disorder MIM:	160777
Disorder Html:	Griscelli syndrome, type 1, 214450 (3)

	MYO5A	XP_001084476.1
Canis lupus familiaris (dog)	MYO5A	XP_005638745.1
Mus musculus (house mouse)	Myo5a	NP_034994.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006479	XP_316519.4
Kluyveromyces lactis	KLLA0A03905g	XP_451171.1
Magnaporthe oryzae (rice blast fungus)	MGG_16213	XP_003709659.1
Arabidopsis thaliana (thale cress)	XIC	NP_172349.2
Oryza sativa (rice)	Os06g0488200	NP_001057670.1
Arabidopsis thaliana (thale cress)	XIE	NP_175858.1
Bos taurus (cattle)	MYO5A	XP_005211933.1
Rattus norvegicus (Norway rat)	Myo5a	NP_071514.1
Gallus gallus (chicken)	MYO5A	NP_990631.1
Neurospora crassa	NCU11354	XP_001728528.1
Xenopus tropicalis (tropical clawed frog)	myo5a	XP_004912752.1
Pan troglodytes (chimpanzee)	MYO5A	XP_510412.2
Danio rerio (zebrafish)	myo5aa	XP_005173731.1
Drosophila melanogaster (fruit fly)	didum	NP_724569.1
Caenorhabditis elegans (roundworm)	hum-2	NP_001023886.1
Saccharomyces cerevisiae (baker's yeast)	MYO2	NP_014971.1
Homo sapiens (human)	MYO5A	NP_000250.3
Eremothecium gossypii	AGOS_ADR354W	NP_984450.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MYO5A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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