MYO5A gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005211878.2	XP_005211935.1	unconventional myosin-Va isoform X4
XM_005211880.2	XP_005211937.1	unconventional myosin-Va isoform X6
XM_015465102.1	XP_015320588.1	unconventional myosin-Va isoform X4
XM_002690962.4	XP_002691008.1	unconventional myosin-Va isoform X3
XM_005211879.2	XP_005211936.1	unconventional myosin-Va isoform X5
XM_015465103.1	XP_015320589.1	unconventional myosin-Va isoform X5
XM_005211877.2	XP_005211934.1	unconventional myosin-Va isoform X2
XM_005211880.3	XP_005211937.1	unconventional myosin-Va isoform X8
XM_015465099.1	XP_015320585.1	unconventional myosin-Va isoform X1
XM_015465101.1	XP_015320587.1	unconventional myosin-Va isoform X3
XM_005211877.3	XP_005211934.1	unconventional myosin-Va isoform X2
XM_024998028.1	XP_024853796.1	unconventional myosin-Va isoform X3
XM_005211879.3	XP_005211936.1	unconventional myosin-Va isoform X6
XM_005211876.2	XP_005211933.1	unconventional myosin-Va isoform X1
XM_015465100.1	XP_015320586.1	unconventional myosin-Va isoform X2
XM_015465104.1	XP_015320590.1	unconventional myosin-Va isoform X6
XM_005211876.3	XP_005211933.1	unconventional myosin-Va isoform X1
XM_002690962.5	XP_002691008.1	unconventional myosin-Va isoform X4
XM_005211878.3	XP_005211935.1	unconventional myosin-Va isoform X5
XM_024998029.1	XP_024853797.1	unconventional myosin-Va isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219879	XM_005211876.3 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa219880	XM_005211877.3 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa219881	XM_024998028.1 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa219882	XM_002690962.5 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa219883	XM_005211878.3 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa219884	XM_005211879.3 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa219885	XM_024998029.1 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa219886	XM_005211880.3 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa23393	XM_005211876.2 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa23393	XM_015465099.1 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa23394	XM_015465100.1 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa23394	XM_005211877.2 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa23395	XM_015465101.1 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa23395	XM_002690962.4 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa23396	XM_015465102.1 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa23396	XM_005211878.2 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa23397	XM_005211879.2 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa23397	XM_015465103.1 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa23398	XM_005211880.2 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa23398	XM_015465104.1 Latest version!	Bos taurus myosin VA (MYO5A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

MYO5A ( XM_005211876.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211933.1 Bos taurus myosin VA (MYO5A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219879
Clone ID Related Accession (Same CDS sequence)	XM_005211876.3
Accession Version	XM_005211876.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211876.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
CCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGTGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCACTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TCGAAAAACA AGATAAGACT GTCCGGAAAC TGAAAAAACA ACTGAAAGTA 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGCCAGATGG AGAACATATC GCCAGGACAA 
ATCATTGATG AACCCATTCG TCCAGTCAAC ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTGA AGAACCTGAT TCTGGAACTG 
AAGCCACGTG GTGTAGCAGT CAATTTGATT CCAGGGTTAC CAGCGTATAT CCTGTTTATG 
TGTGTTCGAC ACGCTGACTA CCTGAATGAT GATCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAATA GCATCAAAAA AGTATTAAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTTTGGCTTT CTAACACATG CCGATTTTTG CACTGTTTAA AGCAGTACAG TGGAGAAGAG 
GGCTTTATGA AACACAACAC CTCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACTTG 
GCTGAGTACC GGCAGGTGCT CAGTGACTTG GCAATTCAGA TCTACCAGCA GCTCGTGAGG 
GTGTTAGAGA ACATCCTTCA GCCAATGATC GTCTCCGGAA TGCTGGAGCA TGAAACCATT 
CAGGGTGTCT CTGGGGTGAA GCCCACAGGG CTAAGAAAGC GAACCTCCAG CATCGCTGAC 
GAGGGTACCT ACACACTGGA CTCCATCCTG CGACAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCACG GCATGGACCC CGAATTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATT 
GTGGGGGCCG TCACCCTGAA CAACCTCCTT TTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTAGAAGAAT GGCTGCGTGA CAAGAATTTG 
ATGAACAGTG GGGCTAAGGA AACCCTGGAG CCTCTCATTC AGGCTGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGACGCGGAA GCCATTTGTT CCATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTCAAAGT GTTGAATTTG TATACACCAG TTAATGAGTT TGAAGAAAGA 
GTTTCTGTGT CATTTATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCT 
CAACTTCTCA TGGATGCAAA ACACATCTTT CCTGTCACCT TTCCTTTTAA CCCCTCTTCC 
CTTGCGCTAG AAACCATCCA GATTCCAGCC AGCCTGGGCC TGGGATTCAT ATCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211933.1
CDS	60..5702
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_005211876.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211876.3

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
CCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGTGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCACTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TCGAAAAACA AGATAAGACT GTCCGGAAAC TGAAAAAACA ACTGAAAGTA 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGCCAGATGG AGAACATATC GCCAGGACAA 
ATCATTGATG AACCCATTCG TCCAGTCAAC ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTGA AGAACCTGAT TCTGGAACTG 
AAGCCACGTG GTGTAGCAGT CAATTTGATT CCAGGGTTAC CAGCGTATAT CCTGTTTATG 
TGTGTTCGAC ACGCTGACTA CCTGAATGAT GATCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAATA GCATCAAAAA AGTATTAAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTTTGGCTTT CTAACACATG CCGATTTTTG CACTGTTTAA AGCAGTACAG TGGAGAAGAG 
GGCTTTATGA AACACAACAC CTCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACTTG 
GCTGAGTACC GGCAGGTGCT CAGTGACTTG GCAATTCAGA TCTACCAGCA GCTCGTGAGG 
GTGTTAGAGA ACATCCTTCA GCCAATGATC GTCTCCGGAA TGCTGGAGCA TGAAACCATT 
CAGGGTGTCT CTGGGGTGAA GCCCACAGGG CTAAGAAAGC GAACCTCCAG CATCGCTGAC 
GAGGGTACCT ACACACTGGA CTCCATCCTG CGACAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCACG GCATGGACCC CGAATTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATT 
GTGGGGGCCG TCACCCTGAA CAACCTCCTT TTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTAGAAGAAT GGCTGCGTGA CAAGAATTTG 
ATGAACAGTG GGGCTAAGGA AACCCTGGAG CCTCTCATTC AGGCTGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGACGCGGAA GCCATTTGTT CCATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTCAAAGT GTTGAATTTG TATACACCAG TTAATGAGTT TGAAGAAAGA 
GTTTCTGTGT CATTTATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCT 
CAACTTCTCA TGGATGCAAA ACACATCTTT CCTGTCACCT TTCCTTTTAA CCCCTCTTCC 
CTTGCGCTAG AAACCATCCA GATTCCAGCC AGCCTGGGCC TGGGATTCAT ATCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_005211877.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211934.1 Bos taurus myosin VA (MYO5A), transcript variant X2, mRNA.

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CloneID	OBa219880
Clone ID Related Accession (Same CDS sequence)	XM_005211877.3
Accession Version	XM_005211877.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211877.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ACCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGTGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCG GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCACTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTCGAAAAAC AAGATAAGAC TGTCCGGAAA CTGAAAAAAC AACTGAAAGT ATTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGCCAGATG GAGAACATAT CGCCAGGACA AATCATTGAT 
GAACCCATTC GTCCAGTCAA CATTCCCAGG AAGGAAAAGG ATTTCCAAGG AATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTG AAGAACCTGA TTCTGGAACT GAAGCCACGT 
GGTGTAGCAG TCAATTTGAT TCCAGGGTTA CCAGCGTATA TCCTGTTTAT GTGTGTTCGA 
CACGCTGACT ACCTGAATGA TGATCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAT 
AGCATCAAAA AAGTATTAAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTTTGGCTT 
TCTAACACAT GCCGATTTTT GCACTGTTTA AAGCAGTACA GTGGAGAAGA GGGCTTTATG 
AAACACAACA CCTCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACTT GGCTGAGTAC 
CGGCAGGTGC TCAGTGACTT GGCAATTCAG ATCTACCAGC AGCTCGTGAG GGTGTTAGAG 
AACATCCTTC AGCCAATGAT CGTCTCCGGA ATGCTGGAGC ATGAAACCAT TCAGGGTGTC 
TCTGGGGTGA AGCCCACAGG GCTAAGAAAG CGAACCTCCA GCATCGCTGA CGAGGGTACC 
TACACACTGG ACTCCATCCT GCGACAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAC 
GGCATGGACC CCGAATTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT TGTGGGGGCC 
GTCACCCTGA ACAACCTCCT TTTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTAGAAGAA TGGCTGCGTG ACAAGAATTT GATGAACAGT 
GGGGCTAAGG AAACCCTGGA GCCTCTCATT CAGGCTGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGACGCGGA AGCCATTTGT TCCATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTCAAAG TGTTGAATTT GTATACACCA GTTAATGAGT TTGAAGAAAG AGTTTCTGTG 
TCATTTATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC TCAACTTCTC 
ATGGATGCAA AACACATCTT TCCTGTCACC TTTCCTTTTA ACCCCTCTTC CCTTGCGCTA 
GAAACCATCC AGATTCCAGC CAGCCTGGGC CTGGGATTCA TATCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211934.1
CDS	60..5693
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_005211877.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211877.3

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ACCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGTGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCG GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCACTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTCGAAAAAC AAGATAAGAC TGTCCGGAAA CTGAAAAAAC AACTGAAAGT ATTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGCCAGATG GAGAACATAT CGCCAGGACA AATCATTGAT 
GAACCCATTC GTCCAGTCAA CATTCCCAGG AAGGAAAAGG ATTTCCAAGG AATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTG AAGAACCTGA TTCTGGAACT GAAGCCACGT 
GGTGTAGCAG TCAATTTGAT TCCAGGGTTA CCAGCGTATA TCCTGTTTAT GTGTGTTCGA 
CACGCTGACT ACCTGAATGA TGATCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAT 
AGCATCAAAA AAGTATTAAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTTTGGCTT 
TCTAACACAT GCCGATTTTT GCACTGTTTA AAGCAGTACA GTGGAGAAGA GGGCTTTATG 
AAACACAACA CCTCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACTT GGCTGAGTAC 
CGGCAGGTGC TCAGTGACTT GGCAATTCAG ATCTACCAGC AGCTCGTGAG GGTGTTAGAG 
AACATCCTTC AGCCAATGAT CGTCTCCGGA ATGCTGGAGC ATGAAACCAT TCAGGGTGTC 
TCTGGGGTGA AGCCCACAGG GCTAAGAAAG CGAACCTCCA GCATCGCTGA CGAGGGTACC 
TACACACTGG ACTCCATCCT GCGACAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAC 
GGCATGGACC CCGAATTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT TGTGGGGGCC 
GTCACCCTGA ACAACCTCCT TTTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTAGAAGAA TGGCTGCGTG ACAAGAATTT GATGAACAGT 
GGGGCTAAGG AAACCCTGGA GCCTCTCATT CAGGCTGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGACGCGGA AGCCATTTGT TCCATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTCAAAG TGTTGAATTT GTATACACCA GTTAATGAGT TTGAAGAAAG AGTTTCTGTG 
TCATTTATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC TCAACTTCTC 
ATGGATGCAA AACACATCTT TCCTGTCACC TTTCCTTTTA ACCCCTCTTC CCTTGCGCTA 
GAAACCATCC AGATTCCAGC CAGCCTGGGC CTGGGATTCA TATCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_024998028.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853796.1 Bos taurus myosin VA (MYO5A), transcript variant X3, mRNA.

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CloneID	OBa219881
Clone ID Related Accession (Same CDS sequence)	XM_024998028.1
Accession Version	XM_024998028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCAGT TTGCCAGGGT TTGGATACCT GATCCTGAAG AAGTTTGGAA GTCAGCAGAG 
CTGCTCAAAG ATTATAAGCC AGGAGATAAA GTTCTCCTGC TTCAACTTGA GGATGGAAAG 
GATTTGGAAT ATCGTCTAGA TCCAAAGACC AAGGAACTGC CTCATTTAAG AAACCCTGAC 
ATACTTGTGG GTGAAAATGA TCTCACAGCC CTCAGTTATC TTCACGAGCC TGCTGTACTC 
CATAATCTCA GAGTCCGCTT TATTGATTCC AAACTTATTT ATACATATTG TGGTATAGTC 
CTAGTAGCTA TAAATCCTTA TGAACAGCTG CCTATTTATG GAGAAGATAT TATTAATGCA 
TATAGTGGTC AGAACATGGG TGATATGGAT CCACATATCT TTGCAGTAGC TGAAGAAGCT 
TACAAGCAAA TGGCCAGAGA TGAACGAAAT CAGTCCATCA TTGTAAGTGG AGAGTCTGGG 
GCAGGAAAAA CAGTTTCAGC TAAGTATGCC ATGAGATACT TTGCAACAGT CAGTGGTTCT 
GCCAGTGAAG CCAATGTTGA GGAAAAGGTG TTGGCTTCCA ACCCCATCAT GGAGTCAATT 
GGAAATGCTA AAACAACCAG GAATGATAAT AGTAGCCGTT TTGGGAAGTA CATTGAGATT 
GGTTTTGATA AGAGATATCG AATCATCGGT GCCAATATGA GAACTTACCT TTTAGAGAAA 
TCCAGAGTGG TGTTCCAGGC AGAAGAGGAG AGAAACTATC ATATCTTCTA TCAACTTTGT 
GCCTCAGCAG ACTTATCTGA ATTTAAAGTG CTGCGATTAG GAGATGCAAA TAACTTTCAT 
TACACGAATC AAGGTGGCAG TCCTGTGATT GAAGGAGTAG ATGATGCCAA GGAGATGGCA 
CATACCAGGC AGGCCTGCAC TTTGCTAGGA ATTAGTGAAT CTCATCAGAT GGGAATTTTC 
CGAATACTTG CTGGCATCCT ACACTTAGGC AATGTTGTGT TTATGTCTCG AGATTCAGAC 
AGCTGCACAA TCCCTCCCAA GCATGAACCC CTCAGCATCT TCTGTGACCT CATGGGTGTG 
GACTTTGAGG AGCTGTGTCA CTGGCTCTGC CATCGGAAGT TGGCCACTGC CACAGAGACG 
TACATCAAGC CCATCTCCAA GCTGCAGGCC ACGAATGCCC GTGATGCATT GGCCAAGCAC 
ATCTATGCCA AGCTCTTTAA CTGGATTGTA GATCATGTCA ATCAGGCACT CCATTCTGCT 
GTCAAACAGC ATTCTTTTAT CGGTGTACTG GACATTTACG GATTTGAAAC ATTTGAGATA 
AATAGTTTTG AACAGTTTTG CATAAATTAT GCAAATGAAA AACTACAGCA ACAATTCAAT 
ATGCACGTTT TCAAATTAGA ACAAGAAGAA TATATGAAAG AACAAATTCC ATGGACACTC 
ATAGATTTTT ATGATAATCA GCCTTGCATT AATCTTATAG AATCTAAACT GGGCATTCTA 
GATTTGCTGG ATGAGGAATG CAAGATGCCT AAAGGTACAG ATGACACTTG GGCTCAAAAA 
TTGTACAACA CACATTTGAA CAAATGTGCA CTATTTGAGA AGCCCCGCCT ATCAAACAAA 
GCTTTCATCA TCCAGCATTT TGCTGACAAA GTGGAATACC AGTGTGAAGG CTTTCTGGAA 
AAGAATAAAG ACACCGTTTT TGAAGAACAA ATTAAAGTTC TTAAATCAAG CAAGTTTAAG 
ATGCTACCAG AATTATTTCA AGACGATGAG AAGGCCATCA GCCCAACCTC AGCCACCTCT 
TCAGGGCGCA CGCCTCTCAC CCGCACCCTT TCAAAGCCCA CCAAAGGCAG ACCAGGCCAG 
ACGGCCAAAG AGCACAAGAA AACAGTGGGG CACCAGTTCC GAAACTCCCT CCACCTGCTT 
ATGGAGACCC TGAATGCCAC TACCCCTCAC TATGTGCGCT GTATCAAGCC TAACGACTTC 
AAGTTCCCAT TCACGTTTGA TGAGAAGAGG GCAGTGCAGC AGCTGAGAGC ATGCGGTGTC 
CTGGAAACCA TTCGGATCAG CGCAGCAGGT TTCCCCTCTC GGTGGACTTA CCAAGAATTT 
TTCAGCCGCT ATCGGGTCCT AATGAAGCAA AAAGATGTGC TAAGTGACAG AAAGCAAACA 
TGCAAGAATG TTTTAGAGAA ACTGATAGTG GACAAGGACA AATACCAGTT TGGGAAGACA 
AAGATCTTTT TCCGGGCTGG TCAAGTGGCC TACCTAGAAA AATTGAGGGC AGACAAGCTG 
AGGGCTGCCT GCATCCGGAT CCAGAAGACG ATCCGAGGGT GGCTGCTGCG GAAGAAGTAC 
CTGCGCATGC GCAAGGCTGC CATCACTGTG CAGAGATATG TGCGAGGCCA CCAGGCCCGA 
TGCTATGCTA AGTTCCTGCG CAGAACCAAG GCAGCCACTA TCATTCAGAA GTACTGGCGC 
ATGTATGTAG CTCGCCGGAG ATACAAGATC ATGCGAACTG CCACAATTGT CCTTCAGTCT 
TATTTGCGAG GCTACTTGGC CAGAAATAGG TATCACAAGA TACTCCGTGA ACACAAAGCA 
GTCATCATTC AGAAATGGGT CCGTGGCTGG CTGGCTCGCA CCTGCTATAG GAGGAGCATC 
CACGCCATCA TCTACCTTCA GTGCTGCTTC CGACGGATGA TGGCCAAGCG TGAGCTGAAG 
AAACTTAAGA TCGAGGCCCG CTCTGTGGAG CGCTATAAGA AGCTCCACAT TGGCATGGAG 
AACAAGATCA TGCAGCTGCA GCGCAAAGTT GATGAGCAGA ACAAAGACTA CAAATGCCTC 
ATGGAGAAAC TGACCAATCT AGAAGGAATA TACAACTCGG AGACCGAGAA ACTACGAAGT 
GATTTAGAAC GTCTTCAGCT AAGTGAAGAA GAAGCTAAGA TTGCTACTGG ACGGGTCCTC 
AGTCTGCAGG AAGAAATTGC CAAGCTACGG AAAGACCTGG AACAAACTCA GTCTGAGAAA 
AAATCCATTG AGGAACATGC TGATAGATAC AAACAAGAAA CTGAGCAGCT GGTGTCAAAT 
CTGAAGGAAG AAAATACTTT GCTGAAGCAG GAAAAAGAGG TCCTCAATCA CCGTATCGTG 
GAGCAGGCTA AGGAGATGAC AGAGACTATG GAGAAGAAGT TAGTAGAAGA AACAAAACAA 
CTGGAACTCG ATCTTAACGA TGAAAGGCTG AGATATCAGA ACCTTCTGAA TGAGTTTAGT 
CGCTTGGAAG AACGCTATGA TGACCTCAAG GAAGAGATGA CTTTGATGGT GAATGTGCCT 
AAACCTGGAC ACAAGAGAAC AGACTCTACC CACAGCAGCA ATGAGTCTGA ATACACCTTT 
AGCTCTGAAA TTGCAGAAAC TGAAGATATT CCATCAAGGA CAGAGGAGCC AAGTGAGAAG 
AAGGTGCCTC TGGACATGTC ATTGTTCCTC AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG 
CAGGAGAAGC AGGTGATGCA GGATGAGCTG GACCGCAAGG AGGAGCAGGT GCTCCGCAGC 
AAGGCAAAGG AAGAAGAAAG ACCACAGATT AGAGGTGCAG AACTGGAATA TGAGTCACTC 
AAGCGTCAAG AACTAGAGTC AGAAAACAAA AAACTGAAAA ATGAGCTAAA TGAGTTACGA 
AAGGCCCTCA GTGAGAAAAG TGCCCCTGAG GTGACTGCCC CAGGCGCGCC AGCCTACCGT 
GTCCTCATGG AGCAGCTGAC GTCTGTGAGT GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC 
CTCATCCTGA GGTCTCAGCT GGTGAGCCAG AAGGAAGCCA TACAACCCAA GGATGACAAG 
AACACAATGA CAGATTCCAC AATACTTTTA GAAGATGTAC AGAAAATGAA AGATAAAGGC 
GAAATAGCAC AAGCATATAT TGGTTTGAAA GAAACCAACA GACCATCTGC TATGGATTGC 
CATGAGTTGA ATGAGGATGG AGAGCTGTTG CTGGTTTATG AAGGGTTAAA ACAAGCCAAC 
AGGCTCCTGG AGTCCCAGCT GCAGTCTCAG AAGAGGAGCC ATGAGAATGA GGCTGAAGCT 
CTCCGTGGGG AAATCCAGAG CCTGAAGGAG GAGAACAACC GGCAGCAGCA GCTGCTGGCC 
CAGAACCTAC AGCTGCCCCC GGAGGCCCGC ATTGAGGCCA GCCTGCAGCA CGAGATCACT 
CGGCTCACCA ATGAGAACCT GTATTTTGAG GAATTATATG CAGATGACCC TAAGAAGTAT 
CAATCATATC GGATTTCACT TTACAAACGG ATGATTGATT TGATGGAACA ACTCGAAAAA 
CAAGATAAGA CTGTCCGGAA ACTGAAAAAA CAACTGAAAG TATTTGCCAA AAAAATTGGT 
GAACTAGAAG TGGGCCAGAT GGAGAACATA TCGCCAGGAC AAATCATTGA TGAACCCATT 
CGTCCAGTCA ACATTCCCAG GAAGGAAAAG GATTTCCAAG GAATGCTGGA ATACAAGAAG 
GAGGATGAGC AAAAACTTGT GAAGAACCTG ATTCTGGAAC TGAAGCCACG TGGTGTAGCA 
GTCAATTTGA TTCCAGGGTT ACCAGCGTAT ATCCTGTTTA TGTGTGTTCG ACACGCTGAC 
TACCTGAATG ATGATCAGAA AGTAAGGTCG TTGCTAACAT CAACAATTAA TAGCATCAAA 
AAAGTATTAA AGAAAAGAGG TGATGATTTT GAAACCGTCT CCTTTTGGCT TTCTAACACA 
TGCCGATTTT TGCACTGTTT AAAGCAGTAC AGTGGAGAAG AGGGCTTTAT GAAACACAAC 
ACCTCTCGCC AGAATGAACA CTGCCTCACC AATTTTGACT TGGCTGAGTA CCGGCAGGTG 
CTCAGTGACT TGGCAATTCA GATCTACCAG CAGCTCGTGA GGGTGTTAGA GAACATCCTT 
CAGCCAATGA TCGTCTCCGG AATGCTGGAG CATGAAACCA TTCAGGGTGT CTCTGGGGTG 
AAGCCCACAG GGCTAAGAAA GCGAACCTCC AGCATCGCTG ACGAGGGTAC CTACACACTG 
GACTCCATCC TGCGACAGCT CAACTCCTTC CACTCGGTCA TGTGTCAGCA CGGCATGGAC 
CCCGAATTGA TCAAGCAGGT GGTCAAGCAG ATGTTCTACA TTGTGGGGGC CGTCACCCTG 
AACAACCTCC TTTTGCGGAA GGACATGTGC TCCTGGAGTA AAGGCATGCA GATCAGGTAC 
AATGTCAGTC AACTAGAAGA ATGGCTGCGT GACAAGAATT TGATGAACAG TGGGGCTAAG 
GAAACCCTGG AGCCTCTCAT TCAGGCTGCT CAGCTTTTGC AAGTGAAAAA GAAAACAGAT 
GATGACGCGG AAGCCATTTG TTCCATGTGC AATGCTTTAA CTACTGCCCA GATTGTCAAA 
GTGTTGAATT TGTATACACC AGTTAATGAG TTTGAAGAAA GAGTTTCTGT GTCATTTATT 
CGTACTATAC AGATGCGTTT ACGAGACAGG AAAGACTCTC CTCAACTTCT CATGGATGCA 
AAACACATCT TTCCTGTCAC CTTTCCTTTT AACCCCTCTT CCCTTGCGCT AGAAACCATC 
CAGATTCCAG CCAGCCTGGG CCTGGGATTC ATATCACGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853796.1
CDS	16..5640
Translation	MCQFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_024998028.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998028.1

ORF Insert Sequence:

ATGTGTCAGT TTGCCAGGGT TTGGATACCT GATCCTGAAG AAGTTTGGAA GTCAGCAGAG 
CTGCTCAAAG ATTATAAGCC AGGAGATAAA GTTCTCCTGC TTCAACTTGA GGATGGAAAG 
GATTTGGAAT ATCGTCTAGA TCCAAAGACC AAGGAACTGC CTCATTTAAG AAACCCTGAC 
ATACTTGTGG GTGAAAATGA TCTCACAGCC CTCAGTTATC TTCACGAGCC TGCTGTACTC 
CATAATCTCA GAGTCCGCTT TATTGATTCC AAACTTATTT ATACATATTG TGGTATAGTC 
CTAGTAGCTA TAAATCCTTA TGAACAGCTG CCTATTTATG GAGAAGATAT TATTAATGCA 
TATAGTGGTC AGAACATGGG TGATATGGAT CCACATATCT TTGCAGTAGC TGAAGAAGCT 
TACAAGCAAA TGGCCAGAGA TGAACGAAAT CAGTCCATCA TTGTAAGTGG AGAGTCTGGG 
GCAGGAAAAA CAGTTTCAGC TAAGTATGCC ATGAGATACT TTGCAACAGT CAGTGGTTCT 
GCCAGTGAAG CCAATGTTGA GGAAAAGGTG TTGGCTTCCA ACCCCATCAT GGAGTCAATT 
GGAAATGCTA AAACAACCAG GAATGATAAT AGTAGCCGTT TTGGGAAGTA CATTGAGATT 
GGTTTTGATA AGAGATATCG AATCATCGGT GCCAATATGA GAACTTACCT TTTAGAGAAA 
TCCAGAGTGG TGTTCCAGGC AGAAGAGGAG AGAAACTATC ATATCTTCTA TCAACTTTGT 
GCCTCAGCAG ACTTATCTGA ATTTAAAGTG CTGCGATTAG GAGATGCAAA TAACTTTCAT 
TACACGAATC AAGGTGGCAG TCCTGTGATT GAAGGAGTAG ATGATGCCAA GGAGATGGCA 
CATACCAGGC AGGCCTGCAC TTTGCTAGGA ATTAGTGAAT CTCATCAGAT GGGAATTTTC 
CGAATACTTG CTGGCATCCT ACACTTAGGC AATGTTGTGT TTATGTCTCG AGATTCAGAC 
AGCTGCACAA TCCCTCCCAA GCATGAACCC CTCAGCATCT TCTGTGACCT CATGGGTGTG 
GACTTTGAGG AGCTGTGTCA CTGGCTCTGC CATCGGAAGT TGGCCACTGC CACAGAGACG 
TACATCAAGC CCATCTCCAA GCTGCAGGCC ACGAATGCCC GTGATGCATT GGCCAAGCAC 
ATCTATGCCA AGCTCTTTAA CTGGATTGTA GATCATGTCA ATCAGGCACT CCATTCTGCT 
GTCAAACAGC ATTCTTTTAT CGGTGTACTG GACATTTACG GATTTGAAAC ATTTGAGATA 
AATAGTTTTG AACAGTTTTG CATAAATTAT GCAAATGAAA AACTACAGCA ACAATTCAAT 
ATGCACGTTT TCAAATTAGA ACAAGAAGAA TATATGAAAG AACAAATTCC ATGGACACTC 
ATAGATTTTT ATGATAATCA GCCTTGCATT AATCTTATAG AATCTAAACT GGGCATTCTA 
GATTTGCTGG ATGAGGAATG CAAGATGCCT AAAGGTACAG ATGACACTTG GGCTCAAAAA 
TTGTACAACA CACATTTGAA CAAATGTGCA CTATTTGAGA AGCCCCGCCT ATCAAACAAA 
GCTTTCATCA TCCAGCATTT TGCTGACAAA GTGGAATACC AGTGTGAAGG CTTTCTGGAA 
AAGAATAAAG ACACCGTTTT TGAAGAACAA ATTAAAGTTC TTAAATCAAG CAAGTTTAAG 
ATGCTACCAG AATTATTTCA AGACGATGAG AAGGCCATCA GCCCAACCTC AGCCACCTCT 
TCAGGGCGCA CGCCTCTCAC CCGCACCCTT TCAAAGCCCA CCAAAGGCAG ACCAGGCCAG 
ACGGCCAAAG AGCACAAGAA AACAGTGGGG CACCAGTTCC GAAACTCCCT CCACCTGCTT 
ATGGAGACCC TGAATGCCAC TACCCCTCAC TATGTGCGCT GTATCAAGCC TAACGACTTC 
AAGTTCCCAT TCACGTTTGA TGAGAAGAGG GCAGTGCAGC AGCTGAGAGC ATGCGGTGTC 
CTGGAAACCA TTCGGATCAG CGCAGCAGGT TTCCCCTCTC GGTGGACTTA CCAAGAATTT 
TTCAGCCGCT ATCGGGTCCT AATGAAGCAA AAAGATGTGC TAAGTGACAG AAAGCAAACA 
TGCAAGAATG TTTTAGAGAA ACTGATAGTG GACAAGGACA AATACCAGTT TGGGAAGACA 
AAGATCTTTT TCCGGGCTGG TCAAGTGGCC TACCTAGAAA AATTGAGGGC AGACAAGCTG 
AGGGCTGCCT GCATCCGGAT CCAGAAGACG ATCCGAGGGT GGCTGCTGCG GAAGAAGTAC 
CTGCGCATGC GCAAGGCTGC CATCACTGTG CAGAGATATG TGCGAGGCCA CCAGGCCCGA 
TGCTATGCTA AGTTCCTGCG CAGAACCAAG GCAGCCACTA TCATTCAGAA GTACTGGCGC 
ATGTATGTAG CTCGCCGGAG ATACAAGATC ATGCGAACTG CCACAATTGT CCTTCAGTCT 
TATTTGCGAG GCTACTTGGC CAGAAATAGG TATCACAAGA TACTCCGTGA ACACAAAGCA 
GTCATCATTC AGAAATGGGT CCGTGGCTGG CTGGCTCGCA CCTGCTATAG GAGGAGCATC 
CACGCCATCA TCTACCTTCA GTGCTGCTTC CGACGGATGA TGGCCAAGCG TGAGCTGAAG 
AAACTTAAGA TCGAGGCCCG CTCTGTGGAG CGCTATAAGA AGCTCCACAT TGGCATGGAG 
AACAAGATCA TGCAGCTGCA GCGCAAAGTT GATGAGCAGA ACAAAGACTA CAAATGCCTC 
ATGGAGAAAC TGACCAATCT AGAAGGAATA TACAACTCGG AGACCGAGAA ACTACGAAGT 
GATTTAGAAC GTCTTCAGCT AAGTGAAGAA GAAGCTAAGA TTGCTACTGG ACGGGTCCTC 
AGTCTGCAGG AAGAAATTGC CAAGCTACGG AAAGACCTGG AACAAACTCA GTCTGAGAAA 
AAATCCATTG AGGAACATGC TGATAGATAC AAACAAGAAA CTGAGCAGCT GGTGTCAAAT 
CTGAAGGAAG AAAATACTTT GCTGAAGCAG GAAAAAGAGG TCCTCAATCA CCGTATCGTG 
GAGCAGGCTA AGGAGATGAC AGAGACTATG GAGAAGAAGT TAGTAGAAGA AACAAAACAA 
CTGGAACTCG ATCTTAACGA TGAAAGGCTG AGATATCAGA ACCTTCTGAA TGAGTTTAGT 
CGCTTGGAAG AACGCTATGA TGACCTCAAG GAAGAGATGA CTTTGATGGT GAATGTGCCT 
AAACCTGGAC ACAAGAGAAC AGACTCTACC CACAGCAGCA ATGAGTCTGA ATACACCTTT 
AGCTCTGAAA TTGCAGAAAC TGAAGATATT CCATCAAGGA CAGAGGAGCC AAGTGAGAAG 
AAGGTGCCTC TGGACATGTC ATTGTTCCTC AAGCTCCAGA AGCGGGTCAC AGAGCTGGAG 
CAGGAGAAGC AGGTGATGCA GGATGAGCTG GACCGCAAGG AGGAGCAGGT GCTCCGCAGC 
AAGGCAAAGG AAGAAGAAAG ACCACAGATT AGAGGTGCAG AACTGGAATA TGAGTCACTC 
AAGCGTCAAG AACTAGAGTC AGAAAACAAA AAACTGAAAA ATGAGCTAAA TGAGTTACGA 
AAGGCCCTCA GTGAGAAAAG TGCCCCTGAG GTGACTGCCC CAGGCGCGCC AGCCTACCGT 
GTCCTCATGG AGCAGCTGAC GTCTGTGAGT GAGGAGCTTG ATGTCCGCAA GGAGGAAGTC 
CTCATCCTGA GGTCTCAGCT GGTGAGCCAG AAGGAAGCCA TACAACCCAA GGATGACAAG 
AACACAATGA CAGATTCCAC AATACTTTTA GAAGATGTAC AGAAAATGAA AGATAAAGGC 
GAAATAGCAC AAGCATATAT TGGTTTGAAA GAAACCAACA GACCATCTGC TATGGATTGC 
CATGAGTTGA ATGAGGATGG AGAGCTGTTG CTGGTTTATG AAGGGTTAAA ACAAGCCAAC 
AGGCTCCTGG AGTCCCAGCT GCAGTCTCAG AAGAGGAGCC ATGAGAATGA GGCTGAAGCT 
CTCCGTGGGG AAATCCAGAG CCTGAAGGAG GAGAACAACC GGCAGCAGCA GCTGCTGGCC 
CAGAACCTAC AGCTGCCCCC GGAGGCCCGC ATTGAGGCCA GCCTGCAGCA CGAGATCACT 
CGGCTCACCA ATGAGAACCT GTATTTTGAG GAATTATATG CAGATGACCC TAAGAAGTAT 
CAATCATATC GGATTTCACT TTACAAACGG ATGATTGATT TGATGGAACA ACTCGAAAAA 
CAAGATAAGA CTGTCCGGAA ACTGAAAAAA CAACTGAAAG TATTTGCCAA AAAAATTGGT 
GAACTAGAAG TGGGCCAGAT GGAGAACATA TCGCCAGGAC AAATCATTGA TGAACCCATT 
CGTCCAGTCA ACATTCCCAG GAAGGAAAAG GATTTCCAAG GAATGCTGGA ATACAAGAAG 
GAGGATGAGC AAAAACTTGT GAAGAACCTG ATTCTGGAAC TGAAGCCACG TGGTGTAGCA 
GTCAATTTGA TTCCAGGGTT ACCAGCGTAT ATCCTGTTTA TGTGTGTTCG ACACGCTGAC 
TACCTGAATG ATGATCAGAA AGTAAGGTCG TTGCTAACAT CAACAATTAA TAGCATCAAA 
AAAGTATTAA AGAAAAGAGG TGATGATTTT GAAACCGTCT CCTTTTGGCT TTCTAACACA 
TGCCGATTTT TGCACTGTTT AAAGCAGTAC AGTGGAGAAG AGGGCTTTAT GAAACACAAC 
ACCTCTCGCC AGAATGAACA CTGCCTCACC AATTTTGACT TGGCTGAGTA CCGGCAGGTG 
CTCAGTGACT TGGCAATTCA GATCTACCAG CAGCTCGTGA GGGTGTTAGA GAACATCCTT 
CAGCCAATGA TCGTCTCCGG AATGCTGGAG CATGAAACCA TTCAGGGTGT CTCTGGGGTG 
AAGCCCACAG GGCTAAGAAA GCGAACCTCC AGCATCGCTG ACGAGGGTAC CTACACACTG 
GACTCCATCC TGCGACAGCT CAACTCCTTC CACTCGGTCA TGTGTCAGCA CGGCATGGAC 
CCCGAATTGA TCAAGCAGGT GGTCAAGCAG ATGTTCTACA TTGTGGGGGC CGTCACCCTG 
AACAACCTCC TTTTGCGGAA GGACATGTGC TCCTGGAGTA AAGGCATGCA GATCAGGTAC 
AATGTCAGTC AACTAGAAGA ATGGCTGCGT GACAAGAATT TGATGAACAG TGGGGCTAAG 
GAAACCCTGG AGCCTCTCAT TCAGGCTGCT CAGCTTTTGC AAGTGAAAAA GAAAACAGAT 
GATGACGCGG AAGCCATTTG TTCCATGTGC AATGCTTTAA CTACTGCCCA GATTGTCAAA 
GTGTTGAATT TGTATACACC AGTTAATGAG TTTGAAGAAA GAGTTTCTGT GTCATTTATT 
CGTACTATAC AGATGCGTTT ACGAGACAGG AAAGACTCTC CTCAACTTCT CATGGATGCA 
AAACACATCT TTCCTGTCAC CTTTCCTTTT AACCCCTCTT CCCTTGCGCT AGAAACCATC 
CAGATTCCAG CCAGCCTGGG CCTGGGATTC ATATCACGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_002690962.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691008.1 Bos taurus myosin VA (MYO5A), transcript variant X4, mRNA.

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CloneID	OBa219882
Clone ID Related Accession (Same CDS sequence)	XM_002690962.5
Accession Version	XM_002690962.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002690962.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
CCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGTGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGG ATTTGATGGA ACAACTCGAA 
AAACAAGATA AGACTGTCCG GAAACTGAAA AAACAACTGA AAGTATTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGCCA GATGGAGAAC ATATCGCCAG GACAAATCAT TGATGAACCC 
ATTCGTCCAG TCAACATTCC CAGGAAGGAA AAGGATTTCC AAGGAATGCT GGAATACAAG 
AAGGAGGATG AGCAAAAACT TGTGAAGAAC CTGATTCTGG AACTGAAGCC ACGTGGTGTA 
GCAGTCAATT TGATTCCAGG GTTACCAGCG TATATCCTGT TTATGTGTGT TCGACACGCT 
GACTACCTGA ATGATGATCA GAAAGTAAGG TCGTTGCTAA CATCAACAAT TAATAGCATC 
AAAAAAGTAT TAAAGAAAAG AGGTGATGAT TTTGAAACCG TCTCCTTTTG GCTTTCTAAC 
ACATGCCGAT TTTTGCACTG TTTAAAGCAG TACAGTGGAG AAGAGGGCTT TATGAAACAC 
AACACCTCTC GCCAGAATGA ACACTGCCTC ACCAATTTTG ACTTGGCTGA GTACCGGCAG 
GTGCTCAGTG ACTTGGCAAT TCAGATCTAC CAGCAGCTCG TGAGGGTGTT AGAGAACATC 
CTTCAGCCAA TGATCGTCTC CGGAATGCTG GAGCATGAAA CCATTCAGGG TGTCTCTGGG 
GTGAAGCCCA CAGGGCTAAG AAAGCGAACC TCCAGCATCG CTGACGAGGG TACCTACACA 
CTGGACTCCA TCCTGCGACA GCTCAACTCC TTCCACTCGG TCATGTGTCA GCACGGCATG 
GACCCCGAAT TGATCAAGCA GGTGGTCAAG CAGATGTTCT ACATTGTGGG GGCCGTCACC 
CTGAACAACC TCCTTTTGCG GAAGGACATG TGCTCCTGGA GTAAAGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTAGA AGAATGGCTG CGTGACAAGA ATTTGATGAA CAGTGGGGCT 
AAGGAAACCC TGGAGCCTCT CATTCAGGCT GCTCAGCTTT TGCAAGTGAA AAAGAAAACA 
GATGATGACG CGGAAGCCAT TTGTTCCATG TGCAATGCTT TAACTACTGC CCAGATTGTC 
AAAGTGTTGA ATTTGTATAC ACCAGTTAAT GAGTTTGAAG AAAGAGTTTC TGTGTCATTT 
ATTCGTACTA TACAGATGCG TTTACGAGAC AGGAAAGACT CTCCTCAACT TCTCATGGAT 
GCAAAACACA TCTTTCCTGT CACCTTTCCT TTTAACCCCT CTTCCCTTGC GCTAGAAACC 
ATCCAGATTC CAGCCAGCCT GGGCCTGGGA TTCATATCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691008.1
CDS	60..5627
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_002690962.5
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002690962.5

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
CCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGTGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGG ATTTGATGGA ACAACTCGAA 
AAACAAGATA AGACTGTCCG GAAACTGAAA AAACAACTGA AAGTATTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGCCA GATGGAGAAC ATATCGCCAG GACAAATCAT TGATGAACCC 
ATTCGTCCAG TCAACATTCC CAGGAAGGAA AAGGATTTCC AAGGAATGCT GGAATACAAG 
AAGGAGGATG AGCAAAAACT TGTGAAGAAC CTGATTCTGG AACTGAAGCC ACGTGGTGTA 
GCAGTCAATT TGATTCCAGG GTTACCAGCG TATATCCTGT TTATGTGTGT TCGACACGCT 
GACTACCTGA ATGATGATCA GAAAGTAAGG TCGTTGCTAA CATCAACAAT TAATAGCATC 
AAAAAAGTAT TAAAGAAAAG AGGTGATGAT TTTGAAACCG TCTCCTTTTG GCTTTCTAAC 
ACATGCCGAT TTTTGCACTG TTTAAAGCAG TACAGTGGAG AAGAGGGCTT TATGAAACAC 
AACACCTCTC GCCAGAATGA ACACTGCCTC ACCAATTTTG ACTTGGCTGA GTACCGGCAG 
GTGCTCAGTG ACTTGGCAAT TCAGATCTAC CAGCAGCTCG TGAGGGTGTT AGAGAACATC 
CTTCAGCCAA TGATCGTCTC CGGAATGCTG GAGCATGAAA CCATTCAGGG TGTCTCTGGG 
GTGAAGCCCA CAGGGCTAAG AAAGCGAACC TCCAGCATCG CTGACGAGGG TACCTACACA 
CTGGACTCCA TCCTGCGACA GCTCAACTCC TTCCACTCGG TCATGTGTCA GCACGGCATG 
GACCCCGAAT TGATCAAGCA GGTGGTCAAG CAGATGTTCT ACATTGTGGG GGCCGTCACC 
CTGAACAACC TCCTTTTGCG GAAGGACATG TGCTCCTGGA GTAAAGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTAGA AGAATGGCTG CGTGACAAGA ATTTGATGAA CAGTGGGGCT 
AAGGAAACCC TGGAGCCTCT CATTCAGGCT GCTCAGCTTT TGCAAGTGAA AAAGAAAACA 
GATGATGACG CGGAAGCCAT TTGTTCCATG TGCAATGCTT TAACTACTGC CCAGATTGTC 
AAAGTGTTGA ATTTGTATAC ACCAGTTAAT GAGTTTGAAG AAAGAGTTTC TGTGTCATTT 
ATTCGTACTA TACAGATGCG TTTACGAGAC AGGAAAGACT CTCCTCAACT TCTCATGGAT 
GCAAAACACA TCTTTCCTGT CACCTTTCCT TTTAACCCCT CTTCCCTTGC GCTAGAAACC 
ATCCAGATTC CAGCCAGCCT GGGCCTGGGA TTCATATCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_005211878.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211935.1 Bos taurus myosin VA (MYO5A), transcript variant X5, mRNA.

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CloneID	OBa219883
Clone ID Related Accession (Same CDS sequence)	XM_005211878.3
Accession Version	XM_005211878.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211878.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGTGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCGGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGTA TTTTGAGGAA TTATATGCAG ATGACCCTAA GAAGTATCAA 
TCATATCGGA TTTCACTTTA CAAACGGATG ATTGATTTGA TGGAACAACT CGAAAAACAA 
GATAAGACTG TCCGGAAACT GAAAAAACAA CTGAAAGTAT TTGCCAAAAA AATTGGTGAA 
CTAGAAGTGG GCCAGATGGA GAACATATCG CCAGGACAAA TCATTGATGA ACCCATTCGT 
CCAGTCAACA TTCCCAGGAA GGAAAAGGAT TTCCAAGGAA TGCTGGAATA CAAGAAGGAG 
GATGAGCAAA AACTTGTGAA GAACCTGATT CTGGAACTGA AGCCACGTGG TGTAGCAGTC 
AATTTGATTC CAGGGTTACC AGCGTATATC CTGTTTATGT GTGTTCGACA CGCTGACTAC 
CTGAATGATG ATCAGAAAGT AAGGTCGTTG CTAACATCAA CAATTAATAG CATCAAAAAA 
GTATTAAAGA AAAGAGGTGA TGATTTTGAA ACCGTCTCCT TTTGGCTTTC TAACACATGC 
CGATTTTTGC ACTGTTTAAA GCAGTACAGT GGAGAAGAGG GCTTTATGAA ACACAACACC 
TCTCGCCAGA ATGAACACTG CCTCACCAAT TTTGACTTGG CTGAGTACCG GCAGGTGCTC 
AGTGACTTGG CAATTCAGAT CTACCAGCAG CTCGTGAGGG TGTTAGAGAA CATCCTTCAG 
CCAATGATCG TCTCCGGAAT GCTGGAGCAT GAAACCATTC AGGGTGTCTC TGGGGTGAAG 
CCCACAGGGC TAAGAAAGCG AACCTCCAGC ATCGCTGACG AGGGTACCTA CACACTGGAC 
TCCATCCTGC GACAGCTCAA CTCCTTCCAC TCGGTCATGT GTCAGCACGG CATGGACCCC 
GAATTGATCA AGCAGGTGGT CAAGCAGATG TTCTACATTG TGGGGGCCGT CACCCTGAAC 
AACCTCCTTT TGCGGAAGGA CATGTGCTCC TGGAGTAAAG GCATGCAGAT CAGGTACAAT 
GTCAGTCAAC TAGAAGAATG GCTGCGTGAC AAGAATTTGA TGAACAGTGG GGCTAAGGAA 
ACCCTGGAGC CTCTCATTCA GGCTGCTCAG CTTTTGCAAG TGAAAAAGAA AACAGATGAT 
GACGCGGAAG CCATTTGTTC CATGTGCAAT GCTTTAACTA CTGCCCAGAT TGTCAAAGTG 
TTGAATTTGT ATACACCAGT TAATGAGTTT GAAGAAAGAG TTTCTGTGTC ATTTATTCGT 
ACTATACAGA TGCGTTTACG AGACAGGAAA GACTCTCCTC AACTTCTCAT GGATGCAAAA 
CACATCTTTC CTGTCACCTT TCCTTTTAAC CCCTCTTCCC TTGCGCTAGA AACCATCCAG 
ATTCCAGCCA GCCTGGGCCT GGGATTCATA TCACGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211935.1
CDS	60..5621
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_005211878.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211878.3

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGTGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCGGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGTA TTTTGAGGAA TTATATGCAG ATGACCCTAA GAAGTATCAA 
TCATATCGGA TTTCACTTTA CAAACGGATG ATTGATTTGA TGGAACAACT CGAAAAACAA 
GATAAGACTG TCCGGAAACT GAAAAAACAA CTGAAAGTAT TTGCCAAAAA AATTGGTGAA 
CTAGAAGTGG GCCAGATGGA GAACATATCG CCAGGACAAA TCATTGATGA ACCCATTCGT 
CCAGTCAACA TTCCCAGGAA GGAAAAGGAT TTCCAAGGAA TGCTGGAATA CAAGAAGGAG 
GATGAGCAAA AACTTGTGAA GAACCTGATT CTGGAACTGA AGCCACGTGG TGTAGCAGTC 
AATTTGATTC CAGGGTTACC AGCGTATATC CTGTTTATGT GTGTTCGACA CGCTGACTAC 
CTGAATGATG ATCAGAAAGT AAGGTCGTTG CTAACATCAA CAATTAATAG CATCAAAAAA 
GTATTAAAGA AAAGAGGTGA TGATTTTGAA ACCGTCTCCT TTTGGCTTTC TAACACATGC 
CGATTTTTGC ACTGTTTAAA GCAGTACAGT GGAGAAGAGG GCTTTATGAA ACACAACACC 
TCTCGCCAGA ATGAACACTG CCTCACCAAT TTTGACTTGG CTGAGTACCG GCAGGTGCTC 
AGTGACTTGG CAATTCAGAT CTACCAGCAG CTCGTGAGGG TGTTAGAGAA CATCCTTCAG 
CCAATGATCG TCTCCGGAAT GCTGGAGCAT GAAACCATTC AGGGTGTCTC TGGGGTGAAG 
CCCACAGGGC TAAGAAAGCG AACCTCCAGC ATCGCTGACG AGGGTACCTA CACACTGGAC 
TCCATCCTGC GACAGCTCAA CTCCTTCCAC TCGGTCATGT GTCAGCACGG CATGGACCCC 
GAATTGATCA AGCAGGTGGT CAAGCAGATG TTCTACATTG TGGGGGCCGT CACCCTGAAC 
AACCTCCTTT TGCGGAAGGA CATGTGCTCC TGGAGTAAAG GCATGCAGAT CAGGTACAAT 
GTCAGTCAAC TAGAAGAATG GCTGCGTGAC AAGAATTTGA TGAACAGTGG GGCTAAGGAA 
ACCCTGGAGC CTCTCATTCA GGCTGCTCAG CTTTTGCAAG TGAAAAAGAA AACAGATGAT 
GACGCGGAAG CCATTTGTTC CATGTGCAAT GCTTTAACTA CTGCCCAGAT TGTCAAAGTG 
TTGAATTTGT ATACACCAGT TAATGAGTTT GAAGAAAGAG TTTCTGTGTC ATTTATTCGT 
ACTATACAGA TGCGTTTACG AGACAGGAAA GACTCTCCTC AACTTCTCAT GGATGCAAAA 
CACATCTTTC CTGTCACCTT TCCTTTTAAC CCCTCTTCCC TTGCGCTAGA AACCATCCAG 
ATTCCAGCCA GCCTGGGCCT GGGATTCATA TCACGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_005211879.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211936.1 Bos taurus myosin VA (MYO5A), transcript variant X6, mRNA.

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CloneID	OBa219884
Clone ID Related Accession (Same CDS sequence)	XM_005211879.3
Accession Version	XM_005211879.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211879.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ACCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGTGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCG GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG GATTTGATGG AACAACTCGA AAAACAAGAT 
AAGACTGTCC GGAAACTGAA AAAACAACTG AAAGTATTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGCC AGATGGAGAA CATATCGCCA GGACAAATCA TTGATGAACC CATTCGTCCA 
GTCAACATTC CCAGGAAGGA AAAGGATTTC CAAGGAATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTGAAGAA CCTGATTCTG GAACTGAAGC CACGTGGTGT AGCAGTCAAT 
TTGATTCCAG GGTTACCAGC GTATATCCTG TTTATGTGTG TTCGACACGC TGACTACCTG 
AATGATGATC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAATAGCAT CAAAAAAGTA 
TTAAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTTT GGCTTTCTAA CACATGCCGA 
TTTTTGCACT GTTTAAAGCA GTACAGTGGA GAAGAGGGCT TTATGAAACA CAACACCTCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACTTGGCTG AGTACCGGCA GGTGCTCAGT 
GACTTGGCAA TTCAGATCTA CCAGCAGCTC GTGAGGGTGT TAGAGAACAT CCTTCAGCCA 
ATGATCGTCT CCGGAATGCT GGAGCATGAA ACCATTCAGG GTGTCTCTGG GGTGAAGCCC 
ACAGGGCTAA GAAAGCGAAC CTCCAGCATC GCTGACGAGG GTACCTACAC ACTGGACTCC 
ATCCTGCGAC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCACGGCAT GGACCCCGAA 
TTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATTGTGG GGGCCGTCAC CCTGAACAAC 
CTCCTTTTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTAG AAGAATGGCT GCGTGACAAG AATTTGATGA ACAGTGGGGC TAAGGAAACC 
CTGGAGCCTC TCATTCAGGC TGCTCAGCTT TTGCAAGTGA AAAAGAAAAC AGATGATGAC 
GCGGAAGCCA TTTGTTCCAT GTGCAATGCT TTAACTACTG CCCAGATTGT CAAAGTGTTG 
AATTTGTATA CACCAGTTAA TGAGTTTGAA GAAAGAGTTT CTGTGTCATT TATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCTCAAC TTCTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACCTTTCC TTTTAACCCC TCTTCCCTTG CGCTAGAAAC CATCCAGATT 
CCAGCCAGCC TGGGCCTGGG ATTCATATCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211936.1
CDS	68..5626
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_005211879.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211879.3

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ACCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGTGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCG GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG GATTTGATGG AACAACTCGA AAAACAAGAT 
AAGACTGTCC GGAAACTGAA AAAACAACTG AAAGTATTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGCC AGATGGAGAA CATATCGCCA GGACAAATCA TTGATGAACC CATTCGTCCA 
GTCAACATTC CCAGGAAGGA AAAGGATTTC CAAGGAATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTGAAGAA CCTGATTCTG GAACTGAAGC CACGTGGTGT AGCAGTCAAT 
TTGATTCCAG GGTTACCAGC GTATATCCTG TTTATGTGTG TTCGACACGC TGACTACCTG 
AATGATGATC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAATAGCAT CAAAAAAGTA 
TTAAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTTT GGCTTTCTAA CACATGCCGA 
TTTTTGCACT GTTTAAAGCA GTACAGTGGA GAAGAGGGCT TTATGAAACA CAACACCTCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACTTGGCTG AGTACCGGCA GGTGCTCAGT 
GACTTGGCAA TTCAGATCTA CCAGCAGCTC GTGAGGGTGT TAGAGAACAT CCTTCAGCCA 
ATGATCGTCT CCGGAATGCT GGAGCATGAA ACCATTCAGG GTGTCTCTGG GGTGAAGCCC 
ACAGGGCTAA GAAAGCGAAC CTCCAGCATC GCTGACGAGG GTACCTACAC ACTGGACTCC 
ATCCTGCGAC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCACGGCAT GGACCCCGAA 
TTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATTGTGG GGGCCGTCAC CCTGAACAAC 
CTCCTTTTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTAG AAGAATGGCT GCGTGACAAG AATTTGATGA ACAGTGGGGC TAAGGAAACC 
CTGGAGCCTC TCATTCAGGC TGCTCAGCTT TTGCAAGTGA AAAAGAAAAC AGATGATGAC 
GCGGAAGCCA TTTGTTCCAT GTGCAATGCT TTAACTACTG CCCAGATTGT CAAAGTGTTG 
AATTTGTATA CACCAGTTAA TGAGTTTGAA GAAAGAGTTT CTGTGTCATT TATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCTCAAC TTCTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACCTTTCC TTTTAACCCC TCTTCCCTTG CGCTAGAAAC CATCCAGATT 
CCAGCCAGCC TGGGCCTGGG ATTCATATCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_024998029.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853797.1 Bos taurus myosin VA (MYO5A), transcript variant X7, mRNA.

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CloneID	OBa219885
Clone ID Related Accession (Same CDS sequence)	XM_024998029.1
Accession Version	XM_024998029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGTGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGT ATTTTGAGGA ATTATATGCA GATGACCCTA AGAAGTATCA ATCATATCGG 
ATTTCACTTT ACAAACGGAT GATTGATTTG ATGGAACAAC TCGAAAAACA AGATAAGACT 
GTCCGGAAAC TGAAAAAACA ACTGAAAGTA TTTGCCAAAA AAATTGGTGA ACTAGAAGTG 
GGCCAGATGG AGAACATATC GCCAGGACAA ATCATTGATG AACCCATTCG TCCAGTCAAC 
ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA ATGCTGGAAT ACAAGAAGGA GGATGAGCAA 
AAACTTGTGA AGAACCTGAT TCTGGAACTG AAGCCACGTG GTGTAGCAGT CAATTTGATT 
CCAGGGTTAC CAGCGTATAT CCTGTTTATG TGTGTTCGAC ACGCTGACTA CCTGAATGAT 
GATCAGAAAG TAAGGTCGTT GCTAACATCA ACAATTAATA GCATCAAAAA AGTATTAAAG 
AAAAGAGGTG ATGATTTTGA AACCGTCTCC TTTTGGCTTT CTAACACATG CCGATTTTTG 
CACTGTTTAA AGCAGTACAG TGGAGAAGAG GGCTTTATGA AACACAACAC CTCTCGCCAG 
AATGAACACT GCCTCACCAA TTTTGACTTG GCTGAGTACC GGCAGGTGCT CAGTGACTTG 
GCAATTCAGA TCTACCAGCA GCTCGTGAGG GTGTTAGAGA ACATCCTTCA GCCAATGATC 
GTCTCCGGAA TGCTGGAGCA TGAAACCATT CAGGGTGTCT CTGGGGTGAA GCCCACAGGG 
CTAAGAAAGC GAACCTCCAG CATCGCTGAC GAGGGTACCT ACACACTGGA CTCCATCCTG 
CGACAGCTCA ACTCCTTCCA CTCGGTCATG TGTCAGCACG GCATGGACCC CGAATTGATC 
AAGCAGGTGG TCAAGCAGAT GTTCTACATT GTGGGGGCCG TCACCCTGAA CAACCTCCTT 
TTGCGGAAGG ACATGTGCTC CTGGAGTAAA GGCATGCAGA TCAGGTACAA TGTCAGTCAA 
CTAGAAGAAT GGCTGCGTGA CAAGAATTTG ATGAACAGTG GGGCTAAGGA AACCCTGGAG 
CCTCTCATTC AGGCTGCTCA GCTTTTGCAA GTGAAAAAGA AAACAGATGA TGACGCGGAA 
GCCATTTGTT CCATGTGCAA TGCTTTAACT ACTGCCCAGA TTGTCAAAGT GTTGAATTTG 
TATACACCAG TTAATGAGTT TGAAGAAAGA GTTTCTGTGT CATTTATTCG TACTATACAG 
ATGCGTTTAC GAGACAGGAA AGACTCTCCT CAACTTCTCA TGGATGCAAA ACACATCTTT 
CCTGTCACCT TTCCTTTTAA CCCCTCTTCC CTTGCGCTAG AAACCATCCA GATTCCAGCC 
AGCCTGGGCC TGGGATTCAT ATCACGGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853797.1
CDS	60..5612
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_024998029.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998029.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG GCTCCTGGAG 
TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT GAGAATGAGG CTGAAGCTCT CCGTGGGGAA 
ATCCAGAGCC TGAAGGAGGA GAACAACCGG CAGCAGCAGC TGCTGGCCCA GAACCTACAG 
CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC CTGCAGCACG AGATCACTCG GCTCACCAAT 
GAGAACCTGT ATTTTGAGGA ATTATATGCA GATGACCCTA AGAAGTATCA ATCATATCGG 
ATTTCACTTT ACAAACGGAT GATTGATTTG ATGGAACAAC TCGAAAAACA AGATAAGACT 
GTCCGGAAAC TGAAAAAACA ACTGAAAGTA TTTGCCAAAA AAATTGGTGA ACTAGAAGTG 
GGCCAGATGG AGAACATATC GCCAGGACAA ATCATTGATG AACCCATTCG TCCAGTCAAC 
ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA ATGCTGGAAT ACAAGAAGGA GGATGAGCAA 
AAACTTGTGA AGAACCTGAT TCTGGAACTG AAGCCACGTG GTGTAGCAGT CAATTTGATT 
CCAGGGTTAC CAGCGTATAT CCTGTTTATG TGTGTTCGAC ACGCTGACTA CCTGAATGAT 
GATCAGAAAG TAAGGTCGTT GCTAACATCA ACAATTAATA GCATCAAAAA AGTATTAAAG 
AAAAGAGGTG ATGATTTTGA AACCGTCTCC TTTTGGCTTT CTAACACATG CCGATTTTTG 
CACTGTTTAA AGCAGTACAG TGGAGAAGAG GGCTTTATGA AACACAACAC CTCTCGCCAG 
AATGAACACT GCCTCACCAA TTTTGACTTG GCTGAGTACC GGCAGGTGCT CAGTGACTTG 
GCAATTCAGA TCTACCAGCA GCTCGTGAGG GTGTTAGAGA ACATCCTTCA GCCAATGATC 
GTCTCCGGAA TGCTGGAGCA TGAAACCATT CAGGGTGTCT CTGGGGTGAA GCCCACAGGG 
CTAAGAAAGC GAACCTCCAG CATCGCTGAC GAGGGTACCT ACACACTGGA CTCCATCCTG 
CGACAGCTCA ACTCCTTCCA CTCGGTCATG TGTCAGCACG GCATGGACCC CGAATTGATC 
AAGCAGGTGG TCAAGCAGAT GTTCTACATT GTGGGGGCCG TCACCCTGAA CAACCTCCTT 
TTGCGGAAGG ACATGTGCTC CTGGAGTAAA GGCATGCAGA TCAGGTACAA TGTCAGTCAA 
CTAGAAGAAT GGCTGCGTGA CAAGAATTTG ATGAACAGTG GGGCTAAGGA AACCCTGGAG 
CCTCTCATTC AGGCTGCTCA GCTTTTGCAA GTGAAAAAGA AAACAGATGA TGACGCGGAA 
GCCATTTGTT CCATGTGCAA TGCTTTAACT ACTGCCCAGA TTGTCAAAGT GTTGAATTTG 
TATACACCAG TTAATGAGTT TGAAGAAAGA GTTTCTGTGT CATTTATTCG TACTATACAG 
ATGCGTTTAC GAGACAGGAA AGACTCTCCT CAACTTCTCA TGGATGCAAA ACACATCTTT 
CCTGTCACCT TTCCTTTTAA CCCCTCTTCC CTTGCGCTAG AAACCATCCA GATTCCAGCC 
AGCCTGGGCC TGGGATTCAT ATCACGGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_005211880.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211937.1 Bos taurus myosin VA (MYO5A), transcript variant X8, mRNA.

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CloneID	OBa219886
Clone ID Related Accession (Same CDS sequence)	XM_005211880.3
Accession Version	XM_005211880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGTGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCGGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGGA TTTGATGGAA CAACTCGAAA AACAAGATAA GACTGTCCGG 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GTGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCGCCAGG ACAAATCATT GATGAACCCA TTCGTCCAGT CAACATTCCC 
AGGAAGGAAA AGGATTTCCA AGGAATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTGAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGG 
TTACCAGCGT ATATCCTGTT TATGTGTGTT CGACACGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AATAGCATCA AAAAAGTATT AAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTTTGG CTTTCTAACA CATGCCGATT TTTGCACTGT 
TTAAAGCAGT ACAGTGGAGA AGAGGGCTTT ATGAAACACA ACACCTCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CTTGGCTGAG TACCGGCAGG TGCTCAGTGA CTTGGCAATT 
CAGATCTACC AGCAGCTCGT GAGGGTGTTA GAGAACATCC TTCAGCCAAT GATCGTCTCC 
GGAATGCTGG AGCATGAAAC CATTCAGGGT GTCTCTGGGG TGAAGCCCAC AGGGCTAAGA 
AAGCGAACCT CCAGCATCGC TGACGAGGGT ACCTACACAC TGGACTCCAT CCTGCGACAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CACGGCATGG ACCCCGAATT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATTGTGGGG GCCGTCACCC TGAACAACCT CCTTTTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTAGAA 
GAATGGCTGC GTGACAAGAA TTTGATGAAC AGTGGGGCTA AGGAAACCCT GGAGCCTCTC 
ATTCAGGCTG CTCAGCTTTT GCAAGTGAAA AAGAAAACAG ATGATGACGC GGAAGCCATT 
TGTTCCATGT GCAATGCTTT AACTACTGCC CAGATTGTCA AAGTGTTGAA TTTGTATACA 
CCAGTTAATG AGTTTGAAGA AAGAGTTTCT GTGTCATTTA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCTCAACTT CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACCTTTCCTT TTAACCCCTC TTCCCTTGCG CTAGAAACCA TCCAGATTCC AGCCAGCCTG 
GGCCTGGGAT TCATATCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211937.1
CDS	60..5546
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_005211880.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211880.3

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTACGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGTGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCGGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGGA TTTGATGGAA CAACTCGAAA AACAAGATAA GACTGTCCGG 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GTGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCGCCAGG ACAAATCATT GATGAACCCA TTCGTCCAGT CAACATTCCC 
AGGAAGGAAA AGGATTTCCA AGGAATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTGAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGG 
TTACCAGCGT ATATCCTGTT TATGTGTGTT CGACACGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AATAGCATCA AAAAAGTATT AAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTTTGG CTTTCTAACA CATGCCGATT TTTGCACTGT 
TTAAAGCAGT ACAGTGGAGA AGAGGGCTTT ATGAAACACA ACACCTCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CTTGGCTGAG TACCGGCAGG TGCTCAGTGA CTTGGCAATT 
CAGATCTACC AGCAGCTCGT GAGGGTGTTA GAGAACATCC TTCAGCCAAT GATCGTCTCC 
GGAATGCTGG AGCATGAAAC CATTCAGGGT GTCTCTGGGG TGAAGCCCAC AGGGCTAAGA 
AAGCGAACCT CCAGCATCGC TGACGAGGGT ACCTACACAC TGGACTCCAT CCTGCGACAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CACGGCATGG ACCCCGAATT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATTGTGGGG GCCGTCACCC TGAACAACCT CCTTTTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTAGAA 
GAATGGCTGC GTGACAAGAA TTTGATGAAC AGTGGGGCTA AGGAAACCCT GGAGCCTCTC 
ATTCAGGCTG CTCAGCTTTT GCAAGTGAAA AAGAAAACAG ATGATGACGC GGAAGCCATT 
TGTTCCATGT GCAATGCTTT AACTACTGCC CAGATTGTCA AAGTGTTGAA TTTGTATACA 
CCAGTTAATG AGTTTGAAGA AAGAGTTTCT GTGTCATTTA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCTCAACTT CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACCTTTCCTT TTAACCCCTC TTCCCTTGCG CTAGAAACCA TCCAGATTCC AGCCAGCCTG 
GGCCTGGGAT TCATATCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015465099.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320585.1 Bos taurus myosin VA (MYO5A), transcript variant X1, mRNA.

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CloneID	OBa23393
Clone ID Related Accession (Same CDS sequence)	XM_015465099.1 , XM_005211876.2
Accession Version	XM_015465099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
CCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGTGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCACTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TCGAAAAACA AGATAAGACT GTCCGGAAAC TGAAAAAACA ACTGAAAGTA 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGCCAGATGG AGAACATATC GCCAGGACAA 
ATCATTGATG AACCCATTCG TCCAGTCAAC ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTGA AGAACCTGAT TCTGGAACTG 
AAGCCACGTG GTGTAGCAGT CAATTTGATT CCAGGGTTAC CAGCGTATAT CCTGTTTATG 
TGTGTTCGAC ACGCTGACTA CCTGAATGAT GATCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAATA GCATCAAAAA AGTATTAAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTTTGGCTTT CTAACACATG CCGATTTTTG CACTGTTTAA AGCAGTACAG TGGAGAAGAG 
GGCTTTATGA AACACAACAC CTCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACTTG 
GCTGAGTACC GGCAGGTGCT CAGTGACTTG GCAATTCAGA TCTACCAGCA GCTCGTGAGG 
GTGTTAGAGA ACATCCTTCA GCCAATGATC GTCTCCGGAA TGCTGGAGCA TGAAACCATT 
CAGGGTGTCT CTGGGGTGAA GCCCACAGGG CTAAGAAAGC GAACCTCCAG CATCGCTGAC 
GAGGGTACCT ACACACTGGA CTCCATCCTG CGACAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCACG GCATGGACCC CGAATTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATT 
GTGGGGGCCG TCACCCTGAA CAACCTCCTT TTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTAGAAGAAT GGCTGCGTGA CAAGAATTTG 
ATGAACAGTG GGGCTAAGGA AACCCTGGAG CCTCTCATTC AGGCTGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGACGCGGAA GCCATTTGTT CCATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTCAAAGT GTTGAATTTG TATACACCAG TTAATGAGTT TGAAGAAAGA 
GTTTCTGTGT CATTTATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCT 
CAACTTCTCA TGGATGCAAA ACACATCTTT CCTGTCACCT TTCCTTTTAA CCCCTCTTCC 
CTTGCGCTAG AAACCATCCA GATTCCAGCC AGCCTGGGCC TGGGATTCAT ATCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320585.1
CDS	137..5779
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015465099.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465099.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
CCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGTGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCACTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TCGAAAAACA AGATAAGACT GTCCGGAAAC TGAAAAAACA ACTGAAAGTA 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGCCAGATGG AGAACATATC GCCAGGACAA 
ATCATTGATG AACCCATTCG TCCAGTCAAC ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTGA AGAACCTGAT TCTGGAACTG 
AAGCCACGTG GTGTAGCAGT CAATTTGATT CCAGGGTTAC CAGCGTATAT CCTGTTTATG 
TGTGTTCGAC ACGCTGACTA CCTGAATGAT GATCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAATA GCATCAAAAA AGTATTAAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTTTGGCTTT CTAACACATG CCGATTTTTG CACTGTTTAA AGCAGTACAG TGGAGAAGAG 
GGCTTTATGA AACACAACAC CTCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACTTG 
GCTGAGTACC GGCAGGTGCT CAGTGACTTG GCAATTCAGA TCTACCAGCA GCTCGTGAGG 
GTGTTAGAGA ACATCCTTCA GCCAATGATC GTCTCCGGAA TGCTGGAGCA TGAAACCATT 
CAGGGTGTCT CTGGGGTGAA GCCCACAGGG CTAAGAAAGC GAACCTCCAG CATCGCTGAC 
GAGGGTACCT ACACACTGGA CTCCATCCTG CGACAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCACG GCATGGACCC CGAATTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATT 
GTGGGGGCCG TCACCCTGAA CAACCTCCTT TTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTAGAAGAAT GGCTGCGTGA CAAGAATTTG 
ATGAACAGTG GGGCTAAGGA AACCCTGGAG CCTCTCATTC AGGCTGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGACGCGGAA GCCATTTGTT CCATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTCAAAGT GTTGAATTTG TATACACCAG TTAATGAGTT TGAAGAAAGA 
GTTTCTGTGT CATTTATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCT 
CAACTTCTCA TGGATGCAAA ACACATCTTT CCTGTCACCT TTCCTTTTAA CCCCTCTTCC 
CTTGCGCTAG AAACCATCCA GATTCCAGCC AGCCTGGGCC TGGGATTCAT ATCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_005211876.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211933.1 Bos taurus myosin VA (MYO5A), transcript variant X1, mRNA.

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CloneID	OBa23393
Clone ID Related Accession (Same CDS sequence)	XM_015465099.1 , XM_005211876.2
Accession Version	XM_005211876.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005211876.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
CCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGTGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCACTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TCGAAAAACA AGATAAGACT GTCCGGAAAC TGAAAAAACA ACTGAAAGTA 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGCCAGATGG AGAACATATC GCCAGGACAA 
ATCATTGATG AACCCATTCG TCCAGTCAAC ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTGA AGAACCTGAT TCTGGAACTG 
AAGCCACGTG GTGTAGCAGT CAATTTGATT CCAGGGTTAC CAGCGTATAT CCTGTTTATG 
TGTGTTCGAC ACGCTGACTA CCTGAATGAT GATCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAATA GCATCAAAAA AGTATTAAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTTTGGCTTT CTAACACATG CCGATTTTTG CACTGTTTAA AGCAGTACAG TGGAGAAGAG 
GGCTTTATGA AACACAACAC CTCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACTTG 
GCTGAGTACC GGCAGGTGCT CAGTGACTTG GCAATTCAGA TCTACCAGCA GCTCGTGAGG 
GTGTTAGAGA ACATCCTTCA GCCAATGATC GTCTCCGGAA TGCTGGAGCA TGAAACCATT 
CAGGGTGTCT CTGGGGTGAA GCCCACAGGG CTAAGAAAGC GAACCTCCAG CATCGCTGAC 
GAGGGTACCT ACACACTGGA CTCCATCCTG CGACAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCACG GCATGGACCC CGAATTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATT 
GTGGGGGCCG TCACCCTGAA CAACCTCCTT TTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTAGAAGAAT GGCTGCGTGA CAAGAATTTG 
ATGAACAGTG GGGCTAAGGA AACCCTGGAG CCTCTCATTC AGGCTGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGACGCGGAA GCCATTTGTT CCATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTCAAAGT GTTGAATTTG TATACACCAG TTAATGAGTT TGAAGAAAGA 
GTTTCTGTGT CATTTATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCT 
CAACTTCTCA TGGATGCAAA ACACATCTTT CCTGTCACCT TTCCTTTTAA CCCCTCTTCC 
CTTGCGCTAG AAACCATCCA GATTCCAGCC AGCCTGGGCC TGGGATTCAT ATCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211933.1
CDS	137..5779
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_005211876.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211876.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
CCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGTGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCATATCGG ATTTCACTTT ACAAACGGAT GATTGATTTG 
ATGGAACAAC TCGAAAAACA AGATAAGACT GTCCGGAAAC TGAAAAAACA ACTGAAAGTA 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGCCAGATGG AGAACATATC GCCAGGACAA 
ATCATTGATG AACCCATTCG TCCAGTCAAC ATTCCCAGGA AGGAAAAGGA TTTCCAAGGA 
ATGCTGGAAT ACAAGAAGGA GGATGAGCAA AAACTTGTGA AGAACCTGAT TCTGGAACTG 
AAGCCACGTG GTGTAGCAGT CAATTTGATT CCAGGGTTAC CAGCGTATAT CCTGTTTATG 
TGTGTTCGAC ACGCTGACTA CCTGAATGAT GATCAGAAAG TAAGGTCGTT GCTAACATCA 
ACAATTAATA GCATCAAAAA AGTATTAAAG AAAAGAGGTG ATGATTTTGA AACCGTCTCC 
TTTTGGCTTT CTAACACATG CCGATTTTTG CACTGTTTAA AGCAGTACAG TGGAGAAGAG 
GGCTTTATGA AACACAACAC CTCTCGCCAG AATGAACACT GCCTCACCAA TTTTGACTTG 
GCTGAGTACC GGCAGGTGCT CAGTGACTTG GCAATTCAGA TCTACCAGCA GCTCGTGAGG 
GTGTTAGAGA ACATCCTTCA GCCAATGATC GTCTCCGGAA TGCTGGAGCA TGAAACCATT 
CAGGGTGTCT CTGGGGTGAA GCCCACAGGG CTAAGAAAGC GAACCTCCAG CATCGCTGAC 
GAGGGTACCT ACACACTGGA CTCCATCCTG CGACAGCTCA ACTCCTTCCA CTCGGTCATG 
TGTCAGCACG GCATGGACCC CGAATTGATC AAGCAGGTGG TCAAGCAGAT GTTCTACATT 
GTGGGGGCCG TCACCCTGAA CAACCTCCTT TTGCGGAAGG ACATGTGCTC CTGGAGTAAA 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTAGAAGAAT GGCTGCGTGA CAAGAATTTG 
ATGAACAGTG GGGCTAAGGA AACCCTGGAG CCTCTCATTC AGGCTGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACAGATGA TGACGCGGAA GCCATTTGTT CCATGTGCAA TGCTTTAACT 
ACTGCCCAGA TTGTCAAAGT GTTGAATTTG TATACACCAG TTAATGAGTT TGAAGAAAGA 
GTTTCTGTGT CATTTATTCG TACTATACAG ATGCGTTTAC GAGACAGGAA AGACTCTCCT 
CAACTTCTCA TGGATGCAAA ACACATCTTT CCTGTCACCT TTCCTTTTAA CCCCTCTTCC 
CTTGCGCTAG AAACCATCCA GATTCCAGCC AGCCTGGGCC TGGGATTCAT ATCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_005211877.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211934.1 Bos taurus myosin VA (MYO5A), transcript variant X2, mRNA.

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CloneID	OBa23394
Clone ID Related Accession (Same CDS sequence)	XM_005211877.2 , XM_015465100.1
Accession Version	XM_005211877.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005211877.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ACCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGTGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCG GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCACTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTCGAAAAAC AAGATAAGAC TGTCCGGAAA CTGAAAAAAC AACTGAAAGT ATTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGCCAGATG GAGAACATAT CGCCAGGACA AATCATTGAT 
GAACCCATTC GTCCAGTCAA CATTCCCAGG AAGGAAAAGG ATTTCCAAGG AATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTG AAGAACCTGA TTCTGGAACT GAAGCCACGT 
GGTGTAGCAG TCAATTTGAT TCCAGGGTTA CCAGCGTATA TCCTGTTTAT GTGTGTTCGA 
CACGCTGACT ACCTGAATGA TGATCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAT 
AGCATCAAAA AAGTATTAAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTTTGGCTT 
TCTAACACAT GCCGATTTTT GCACTGTTTA AAGCAGTACA GTGGAGAAGA GGGCTTTATG 
AAACACAACA CCTCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACTT GGCTGAGTAC 
CGGCAGGTGC TCAGTGACTT GGCAATTCAG ATCTACCAGC AGCTCGTGAG GGTGTTAGAG 
AACATCCTTC AGCCAATGAT CGTCTCCGGA ATGCTGGAGC ATGAAACCAT TCAGGGTGTC 
TCTGGGGTGA AGCCCACAGG GCTAAGAAAG CGAACCTCCA GCATCGCTGA CGAGGGTACC 
TACACACTGG ACTCCATCCT GCGACAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAC 
GGCATGGACC CCGAATTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT TGTGGGGGCC 
GTCACCCTGA ACAACCTCCT TTTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTAGAAGAA TGGCTGCGTG ACAAGAATTT GATGAACAGT 
GGGGCTAAGG AAACCCTGGA GCCTCTCATT CAGGCTGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGACGCGGA AGCCATTTGT TCCATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTCAAAG TGTTGAATTT GTATACACCA GTTAATGAGT TTGAAGAAAG AGTTTCTGTG 
TCATTTATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC TCAACTTCTC 
ATGGATGCAA AACACATCTT TCCTGTCACC TTTCCTTTTA ACCCCTCTTC CCTTGCGCTA 
GAAACCATCC AGATTCCAGC CAGCCTGGGC CTGGGATTCA TATCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211934.1
CDS	137..5770
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_005211877.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211877.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ACCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGTGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCG GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCACTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTCGAAAAAC AAGATAAGAC TGTCCGGAAA CTGAAAAAAC AACTGAAAGT ATTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGCCAGATG GAGAACATAT CGCCAGGACA AATCATTGAT 
GAACCCATTC GTCCAGTCAA CATTCCCAGG AAGGAAAAGG ATTTCCAAGG AATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTG AAGAACCTGA TTCTGGAACT GAAGCCACGT 
GGTGTAGCAG TCAATTTGAT TCCAGGGTTA CCAGCGTATA TCCTGTTTAT GTGTGTTCGA 
CACGCTGACT ACCTGAATGA TGATCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAT 
AGCATCAAAA AAGTATTAAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTTTGGCTT 
TCTAACACAT GCCGATTTTT GCACTGTTTA AAGCAGTACA GTGGAGAAGA GGGCTTTATG 
AAACACAACA CCTCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACTT GGCTGAGTAC 
CGGCAGGTGC TCAGTGACTT GGCAATTCAG ATCTACCAGC AGCTCGTGAG GGTGTTAGAG 
AACATCCTTC AGCCAATGAT CGTCTCCGGA ATGCTGGAGC ATGAAACCAT TCAGGGTGTC 
TCTGGGGTGA AGCCCACAGG GCTAAGAAAG CGAACCTCCA GCATCGCTGA CGAGGGTACC 
TACACACTGG ACTCCATCCT GCGACAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAC 
GGCATGGACC CCGAATTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT TGTGGGGGCC 
GTCACCCTGA ACAACCTCCT TTTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTAGAAGAA TGGCTGCGTG ACAAGAATTT GATGAACAGT 
GGGGCTAAGG AAACCCTGGA GCCTCTCATT CAGGCTGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGACGCGGA AGCCATTTGT TCCATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTCAAAG TGTTGAATTT GTATACACCA GTTAATGAGT TTGAAGAAAG AGTTTCTGTG 
TCATTTATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC TCAACTTCTC 
ATGGATGCAA AACACATCTT TCCTGTCACC TTTCCTTTTA ACCCCTCTTC CCTTGCGCTA 
GAAACCATCC AGATTCCAGC CAGCCTGGGC CTGGGATTCA TATCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015465100.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320586.1 Bos taurus myosin VA (MYO5A), transcript variant X2, mRNA.

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CloneID	OBa23394
Clone ID Related Accession (Same CDS sequence)	XM_005211877.2 , XM_015465100.1
Accession Version	XM_015465100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ACCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGTGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCG GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCACTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTCGAAAAAC AAGATAAGAC TGTCCGGAAA CTGAAAAAAC AACTGAAAGT ATTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGCCAGATG GAGAACATAT CGCCAGGACA AATCATTGAT 
GAACCCATTC GTCCAGTCAA CATTCCCAGG AAGGAAAAGG ATTTCCAAGG AATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTG AAGAACCTGA TTCTGGAACT GAAGCCACGT 
GGTGTAGCAG TCAATTTGAT TCCAGGGTTA CCAGCGTATA TCCTGTTTAT GTGTGTTCGA 
CACGCTGACT ACCTGAATGA TGATCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAT 
AGCATCAAAA AAGTATTAAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTTTGGCTT 
TCTAACACAT GCCGATTTTT GCACTGTTTA AAGCAGTACA GTGGAGAAGA GGGCTTTATG 
AAACACAACA CCTCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACTT GGCTGAGTAC 
CGGCAGGTGC TCAGTGACTT GGCAATTCAG ATCTACCAGC AGCTCGTGAG GGTGTTAGAG 
AACATCCTTC AGCCAATGAT CGTCTCCGGA ATGCTGGAGC ATGAAACCAT TCAGGGTGTC 
TCTGGGGTGA AGCCCACAGG GCTAAGAAAG CGAACCTCCA GCATCGCTGA CGAGGGTACC 
TACACACTGG ACTCCATCCT GCGACAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAC 
GGCATGGACC CCGAATTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT TGTGGGGGCC 
GTCACCCTGA ACAACCTCCT TTTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTAGAAGAA TGGCTGCGTG ACAAGAATTT GATGAACAGT 
GGGGCTAAGG AAACCCTGGA GCCTCTCATT CAGGCTGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGACGCGGA AGCCATTTGT TCCATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTCAAAG TGTTGAATTT GTATACACCA GTTAATGAGT TTGAAGAAAG AGTTTCTGTG 
TCATTTATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC TCAACTTCTC 
ATGGATGCAA AACACATCTT TCCTGTCACC TTTCCTTTTA ACCCCTCTTC CCTTGCGCTA 
GAAACCATCC AGATTCCAGC CAGCCTGGGC CTGGGATTCA TATCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320586.1
CDS	137..5770
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015465100.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465100.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ACCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGTGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCG GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCATATCG GATTTCACTT TACAAACGGA TGATTGATTT GATGGAACAA 
CTCGAAAAAC AAGATAAGAC TGTCCGGAAA CTGAAAAAAC AACTGAAAGT ATTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGCCAGATG GAGAACATAT CGCCAGGACA AATCATTGAT 
GAACCCATTC GTCCAGTCAA CATTCCCAGG AAGGAAAAGG ATTTCCAAGG AATGCTGGAA 
TACAAGAAGG AGGATGAGCA AAAACTTGTG AAGAACCTGA TTCTGGAACT GAAGCCACGT 
GGTGTAGCAG TCAATTTGAT TCCAGGGTTA CCAGCGTATA TCCTGTTTAT GTGTGTTCGA 
CACGCTGACT ACCTGAATGA TGATCAGAAA GTAAGGTCGT TGCTAACATC AACAATTAAT 
AGCATCAAAA AAGTATTAAA GAAAAGAGGT GATGATTTTG AAACCGTCTC CTTTTGGCTT 
TCTAACACAT GCCGATTTTT GCACTGTTTA AAGCAGTACA GTGGAGAAGA GGGCTTTATG 
AAACACAACA CCTCTCGCCA GAATGAACAC TGCCTCACCA ATTTTGACTT GGCTGAGTAC 
CGGCAGGTGC TCAGTGACTT GGCAATTCAG ATCTACCAGC AGCTCGTGAG GGTGTTAGAG 
AACATCCTTC AGCCAATGAT CGTCTCCGGA ATGCTGGAGC ATGAAACCAT TCAGGGTGTC 
TCTGGGGTGA AGCCCACAGG GCTAAGAAAG CGAACCTCCA GCATCGCTGA CGAGGGTACC 
TACACACTGG ACTCCATCCT GCGACAGCTC AACTCCTTCC ACTCGGTCAT GTGTCAGCAC 
GGCATGGACC CCGAATTGAT CAAGCAGGTG GTCAAGCAGA TGTTCTACAT TGTGGGGGCC 
GTCACCCTGA ACAACCTCCT TTTGCGGAAG GACATGTGCT CCTGGAGTAA AGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTAGAAGAA TGGCTGCGTG ACAAGAATTT GATGAACAGT 
GGGGCTAAGG AAACCCTGGA GCCTCTCATT CAGGCTGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACAGATG ATGACGCGGA AGCCATTTGT TCCATGTGCA ATGCTTTAAC TACTGCCCAG 
ATTGTCAAAG TGTTGAATTT GTATACACCA GTTAATGAGT TTGAAGAAAG AGTTTCTGTG 
TCATTTATTC GTACTATACA GATGCGTTTA CGAGACAGGA AAGACTCTCC TCAACTTCTC 
ATGGATGCAA AACACATCTT TCCTGTCACC TTTCCTTTTA ACCCCTCTTC CCTTGCGCTA 
GAAACCATCC AGATTCCAGC CAGCCTGGGC CTGGGATTCA TATCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_002690962.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691008.1 Bos taurus myosin VA (MYO5A), transcript variant X3, mRNA.

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CloneID	OBa23395
Clone ID Related Accession (Same CDS sequence)	XM_002690962.4 , XM_015465101.1
Accession Version	XM_002690962.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_002690962.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
CCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGTGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGG ATTTGATGGA ACAACTCGAA 
AAACAAGATA AGACTGTCCG GAAACTGAAA AAACAACTGA AAGTATTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGCCA GATGGAGAAC ATATCGCCAG GACAAATCAT TGATGAACCC 
ATTCGTCCAG TCAACATTCC CAGGAAGGAA AAGGATTTCC AAGGAATGCT GGAATACAAG 
AAGGAGGATG AGCAAAAACT TGTGAAGAAC CTGATTCTGG AACTGAAGCC ACGTGGTGTA 
GCAGTCAATT TGATTCCAGG GTTACCAGCG TATATCCTGT TTATGTGTGT TCGACACGCT 
GACTACCTGA ATGATGATCA GAAAGTAAGG TCGTTGCTAA CATCAACAAT TAATAGCATC 
AAAAAAGTAT TAAAGAAAAG AGGTGATGAT TTTGAAACCG TCTCCTTTTG GCTTTCTAAC 
ACATGCCGAT TTTTGCACTG TTTAAAGCAG TACAGTGGAG AAGAGGGCTT TATGAAACAC 
AACACCTCTC GCCAGAATGA ACACTGCCTC ACCAATTTTG ACTTGGCTGA GTACCGGCAG 
GTGCTCAGTG ACTTGGCAAT TCAGATCTAC CAGCAGCTCG TGAGGGTGTT AGAGAACATC 
CTTCAGCCAA TGATCGTCTC CGGAATGCTG GAGCATGAAA CCATTCAGGG TGTCTCTGGG 
GTGAAGCCCA CAGGGCTAAG AAAGCGAACC TCCAGCATCG CTGACGAGGG TACCTACACA 
CTGGACTCCA TCCTGCGACA GCTCAACTCC TTCCACTCGG TCATGTGTCA GCACGGCATG 
GACCCCGAAT TGATCAAGCA GGTGGTCAAG CAGATGTTCT ACATTGTGGG GGCCGTCACC 
CTGAACAACC TCCTTTTGCG GAAGGACATG TGCTCCTGGA GTAAAGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTAGA AGAATGGCTG CGTGACAAGA ATTTGATGAA CAGTGGGGCT 
AAGGAAACCC TGGAGCCTCT CATTCAGGCT GCTCAGCTTT TGCAAGTGAA AAAGAAAACA 
GATGATGACG CGGAAGCCAT TTGTTCCATG TGCAATGCTT TAACTACTGC CCAGATTGTC 
AAAGTGTTGA ATTTGTATAC ACCAGTTAAT GAGTTTGAAG AAAGAGTTTC TGTGTCATTT 
ATTCGTACTA TACAGATGCG TTTACGAGAC AGGAAAGACT CTCCTCAACT TCTCATGGAT 
GCAAAACACA TCTTTCCTGT CACCTTTCCT TTTAACCCCT CTTCCCTTGC GCTAGAAACC 
ATCCAGATTC CAGCCAGCCT GGGCCTGGGA TTCATATCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691008.1
CDS	137..5704
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_002690962.4
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002690962.4

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
CCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGTGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGG ATTTGATGGA ACAACTCGAA 
AAACAAGATA AGACTGTCCG GAAACTGAAA AAACAACTGA AAGTATTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGCCA GATGGAGAAC ATATCGCCAG GACAAATCAT TGATGAACCC 
ATTCGTCCAG TCAACATTCC CAGGAAGGAA AAGGATTTCC AAGGAATGCT GGAATACAAG 
AAGGAGGATG AGCAAAAACT TGTGAAGAAC CTGATTCTGG AACTGAAGCC ACGTGGTGTA 
GCAGTCAATT TGATTCCAGG GTTACCAGCG TATATCCTGT TTATGTGTGT TCGACACGCT 
GACTACCTGA ATGATGATCA GAAAGTAAGG TCGTTGCTAA CATCAACAAT TAATAGCATC 
AAAAAAGTAT TAAAGAAAAG AGGTGATGAT TTTGAAACCG TCTCCTTTTG GCTTTCTAAC 
ACATGCCGAT TTTTGCACTG TTTAAAGCAG TACAGTGGAG AAGAGGGCTT TATGAAACAC 
AACACCTCTC GCCAGAATGA ACACTGCCTC ACCAATTTTG ACTTGGCTGA GTACCGGCAG 
GTGCTCAGTG ACTTGGCAAT TCAGATCTAC CAGCAGCTCG TGAGGGTGTT AGAGAACATC 
CTTCAGCCAA TGATCGTCTC CGGAATGCTG GAGCATGAAA CCATTCAGGG TGTCTCTGGG 
GTGAAGCCCA CAGGGCTAAG AAAGCGAACC TCCAGCATCG CTGACGAGGG TACCTACACA 
CTGGACTCCA TCCTGCGACA GCTCAACTCC TTCCACTCGG TCATGTGTCA GCACGGCATG 
GACCCCGAAT TGATCAAGCA GGTGGTCAAG CAGATGTTCT ACATTGTGGG GGCCGTCACC 
CTGAACAACC TCCTTTTGCG GAAGGACATG TGCTCCTGGA GTAAAGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTAGA AGAATGGCTG CGTGACAAGA ATTTGATGAA CAGTGGGGCT 
AAGGAAACCC TGGAGCCTCT CATTCAGGCT GCTCAGCTTT TGCAAGTGAA AAAGAAAACA 
GATGATGACG CGGAAGCCAT TTGTTCCATG TGCAATGCTT TAACTACTGC CCAGATTGTC 
AAAGTGTTGA ATTTGTATAC ACCAGTTAAT GAGTTTGAAG AAAGAGTTTC TGTGTCATTT 
ATTCGTACTA TACAGATGCG TTTACGAGAC AGGAAAGACT CTCCTCAACT TCTCATGGAT 
GCAAAACACA TCTTTCCTGT CACCTTTCCT TTTAACCCCT CTTCCCTTGC GCTAGAAACC 
ATCCAGATTC CAGCCAGCCT GGGCCTGGGA TTCATATCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015465101.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320587.1 Bos taurus myosin VA (MYO5A), transcript variant X3, mRNA.

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CloneID	OBa23395
Clone ID Related Accession (Same CDS sequence)	XM_002690962.4 , XM_015465101.1
Accession Version	XM_015465101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
CCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGTGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGG ATTTGATGGA ACAACTCGAA 
AAACAAGATA AGACTGTCCG GAAACTGAAA AAACAACTGA AAGTATTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGCCA GATGGAGAAC ATATCGCCAG GACAAATCAT TGATGAACCC 
ATTCGTCCAG TCAACATTCC CAGGAAGGAA AAGGATTTCC AAGGAATGCT GGAATACAAG 
AAGGAGGATG AGCAAAAACT TGTGAAGAAC CTGATTCTGG AACTGAAGCC ACGTGGTGTA 
GCAGTCAATT TGATTCCAGG GTTACCAGCG TATATCCTGT TTATGTGTGT TCGACACGCT 
GACTACCTGA ATGATGATCA GAAAGTAAGG TCGTTGCTAA CATCAACAAT TAATAGCATC 
AAAAAAGTAT TAAAGAAAAG AGGTGATGAT TTTGAAACCG TCTCCTTTTG GCTTTCTAAC 
ACATGCCGAT TTTTGCACTG TTTAAAGCAG TACAGTGGAG AAGAGGGCTT TATGAAACAC 
AACACCTCTC GCCAGAATGA ACACTGCCTC ACCAATTTTG ACTTGGCTGA GTACCGGCAG 
GTGCTCAGTG ACTTGGCAAT TCAGATCTAC CAGCAGCTCG TGAGGGTGTT AGAGAACATC 
CTTCAGCCAA TGATCGTCTC CGGAATGCTG GAGCATGAAA CCATTCAGGG TGTCTCTGGG 
GTGAAGCCCA CAGGGCTAAG AAAGCGAACC TCCAGCATCG CTGACGAGGG TACCTACACA 
CTGGACTCCA TCCTGCGACA GCTCAACTCC TTCCACTCGG TCATGTGTCA GCACGGCATG 
GACCCCGAAT TGATCAAGCA GGTGGTCAAG CAGATGTTCT ACATTGTGGG GGCCGTCACC 
CTGAACAACC TCCTTTTGCG GAAGGACATG TGCTCCTGGA GTAAAGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTAGA AGAATGGCTG CGTGACAAGA ATTTGATGAA CAGTGGGGCT 
AAGGAAACCC TGGAGCCTCT CATTCAGGCT GCTCAGCTTT TGCAAGTGAA AAAGAAAACA 
GATGATGACG CGGAAGCCAT TTGTTCCATG TGCAATGCTT TAACTACTGC CCAGATTGTC 
AAAGTGTTGA ATTTGTATAC ACCAGTTAAT GAGTTTGAAG AAAGAGTTTC TGTGTCATTT 
ATTCGTACTA TACAGATGCG TTTACGAGAC AGGAAAGACT CTCCTCAACT TCTCATGGAT 
GCAAAACACA TCTTTCCTGT CACCTTTCCT TTTAACCCCT CTTCCCTTGC GCTAGAAACC 
ATCCAGATTC CAGCCAGCCT GGGCCTGGGA TTCATATCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320587.1
CDS	137..5704
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015465101.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465101.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGA 
CCATCTGCTA TGGATTGCCA TGAGTTGAAT GAGGATGGAG AGCTGTTGCT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCCTGGAG TCCCAGCTGC AGTCTCAGAA GAGGAGCCAT 
GAGAATGAGG CTGAAGCTCT CCGTGGGGAA ATCCAGAGCC TGAAGGAGGA GAACAACCGG 
CAGCAGCAGC TGCTGGCCCA GAACCTACAG CTGCCCCCGG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCACG AGATCACTCG GCTCACCAAT GAGAACCTGG ATTTGATGGA ACAACTCGAA 
AAACAAGATA AGACTGTCCG GAAACTGAAA AAACAACTGA AAGTATTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGCCA GATGGAGAAC ATATCGCCAG GACAAATCAT TGATGAACCC 
ATTCGTCCAG TCAACATTCC CAGGAAGGAA AAGGATTTCC AAGGAATGCT GGAATACAAG 
AAGGAGGATG AGCAAAAACT TGTGAAGAAC CTGATTCTGG AACTGAAGCC ACGTGGTGTA 
GCAGTCAATT TGATTCCAGG GTTACCAGCG TATATCCTGT TTATGTGTGT TCGACACGCT 
GACTACCTGA ATGATGATCA GAAAGTAAGG TCGTTGCTAA CATCAACAAT TAATAGCATC 
AAAAAAGTAT TAAAGAAAAG AGGTGATGAT TTTGAAACCG TCTCCTTTTG GCTTTCTAAC 
ACATGCCGAT TTTTGCACTG TTTAAAGCAG TACAGTGGAG AAGAGGGCTT TATGAAACAC 
AACACCTCTC GCCAGAATGA ACACTGCCTC ACCAATTTTG ACTTGGCTGA GTACCGGCAG 
GTGCTCAGTG ACTTGGCAAT TCAGATCTAC CAGCAGCTCG TGAGGGTGTT AGAGAACATC 
CTTCAGCCAA TGATCGTCTC CGGAATGCTG GAGCATGAAA CCATTCAGGG TGTCTCTGGG 
GTGAAGCCCA CAGGGCTAAG AAAGCGAACC TCCAGCATCG CTGACGAGGG TACCTACACA 
CTGGACTCCA TCCTGCGACA GCTCAACTCC TTCCACTCGG TCATGTGTCA GCACGGCATG 
GACCCCGAAT TGATCAAGCA GGTGGTCAAG CAGATGTTCT ACATTGTGGG GGCCGTCACC 
CTGAACAACC TCCTTTTGCG GAAGGACATG TGCTCCTGGA GTAAAGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTAGA AGAATGGCTG CGTGACAAGA ATTTGATGAA CAGTGGGGCT 
AAGGAAACCC TGGAGCCTCT CATTCAGGCT GCTCAGCTTT TGCAAGTGAA AAAGAAAACA 
GATGATGACG CGGAAGCCAT TTGTTCCATG TGCAATGCTT TAACTACTGC CCAGATTGTC 
AAAGTGTTGA ATTTGTATAC ACCAGTTAAT GAGTTTGAAG AAAGAGTTTC TGTGTCATTT 
ATTCGTACTA TACAGATGCG TTTACGAGAC AGGAAAGACT CTCCTCAACT TCTCATGGAT 
GCAAAACACA TCTTTCCTGT CACCTTTCCT TTTAACCCCT CTTCCCTTGC GCTAGAAACC 
ATCCAGATTC CAGCCAGCCT GGGCCTGGGA TTCATATCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_005211878.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211935.1 Bos taurus myosin VA (MYO5A), transcript variant X4, mRNA.

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CloneID	OBa23396
Clone ID Related Accession (Same CDS sequence)	XM_005211878.2 , XM_015465102.1
Accession Version	XM_005211878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005211878.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGTGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCGGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGTA TTTTGAGGAA TTATATGCAG ATGACCCTAA GAAGTATCAA 
TCATATCGGA TTTCACTTTA CAAACGGATG ATTGATTTGA TGGAACAACT CGAAAAACAA 
GATAAGACTG TCCGGAAACT GAAAAAACAA CTGAAAGTAT TTGCCAAAAA AATTGGTGAA 
CTAGAAGTGG GCCAGATGGA GAACATATCG CCAGGACAAA TCATTGATGA ACCCATTCGT 
CCAGTCAACA TTCCCAGGAA GGAAAAGGAT TTCCAAGGAA TGCTGGAATA CAAGAAGGAG 
GATGAGCAAA AACTTGTGAA GAACCTGATT CTGGAACTGA AGCCACGTGG TGTAGCAGTC 
AATTTGATTC CAGGGTTACC AGCGTATATC CTGTTTATGT GTGTTCGACA CGCTGACTAC 
CTGAATGATG ATCAGAAAGT AAGGTCGTTG CTAACATCAA CAATTAATAG CATCAAAAAA 
GTATTAAAGA AAAGAGGTGA TGATTTTGAA ACCGTCTCCT TTTGGCTTTC TAACACATGC 
CGATTTTTGC ACTGTTTAAA GCAGTACAGT GGAGAAGAGG GCTTTATGAA ACACAACACC 
TCTCGCCAGA ATGAACACTG CCTCACCAAT TTTGACTTGG CTGAGTACCG GCAGGTGCTC 
AGTGACTTGG CAATTCAGAT CTACCAGCAG CTCGTGAGGG TGTTAGAGAA CATCCTTCAG 
CCAATGATCG TCTCCGGAAT GCTGGAGCAT GAAACCATTC AGGGTGTCTC TGGGGTGAAG 
CCCACAGGGC TAAGAAAGCG AACCTCCAGC ATCGCTGACG AGGGTACCTA CACACTGGAC 
TCCATCCTGC GACAGCTCAA CTCCTTCCAC TCGGTCATGT GTCAGCACGG CATGGACCCC 
GAATTGATCA AGCAGGTGGT CAAGCAGATG TTCTACATTG TGGGGGCCGT CACCCTGAAC 
AACCTCCTTT TGCGGAAGGA CATGTGCTCC TGGAGTAAAG GCATGCAGAT CAGGTACAAT 
GTCAGTCAAC TAGAAGAATG GCTGCGTGAC AAGAATTTGA TGAACAGTGG GGCTAAGGAA 
ACCCTGGAGC CTCTCATTCA GGCTGCTCAG CTTTTGCAAG TGAAAAAGAA AACAGATGAT 
GACGCGGAAG CCATTTGTTC CATGTGCAAT GCTTTAACTA CTGCCCAGAT TGTCAAAGTG 
TTGAATTTGT ATACACCAGT TAATGAGTTT GAAGAAAGAG TTTCTGTGTC ATTTATTCGT 
ACTATACAGA TGCGTTTACG AGACAGGAAA GACTCTCCTC AACTTCTCAT GGATGCAAAA 
CACATCTTTC CTGTCACCTT TCCTTTTAAC CCCTCTTCCC TTGCGCTAGA AACCATCCAG 
ATTCCAGCCA GCCTGGGCCT GGGATTCATA TCACGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211935.1
CDS	137..5698
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_005211878.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211878.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGTGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCGGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGTA TTTTGAGGAA TTATATGCAG ATGACCCTAA GAAGTATCAA 
TCATATCGGA TTTCACTTTA CAAACGGATG ATTGATTTGA TGGAACAACT CGAAAAACAA 
GATAAGACTG TCCGGAAACT GAAAAAACAA CTGAAAGTAT TTGCCAAAAA AATTGGTGAA 
CTAGAAGTGG GCCAGATGGA GAACATATCG CCAGGACAAA TCATTGATGA ACCCATTCGT 
CCAGTCAACA TTCCCAGGAA GGAAAAGGAT TTCCAAGGAA TGCTGGAATA CAAGAAGGAG 
GATGAGCAAA AACTTGTGAA GAACCTGATT CTGGAACTGA AGCCACGTGG TGTAGCAGTC 
AATTTGATTC CAGGGTTACC AGCGTATATC CTGTTTATGT GTGTTCGACA CGCTGACTAC 
CTGAATGATG ATCAGAAAGT AAGGTCGTTG CTAACATCAA CAATTAATAG CATCAAAAAA 
GTATTAAAGA AAAGAGGTGA TGATTTTGAA ACCGTCTCCT TTTGGCTTTC TAACACATGC 
CGATTTTTGC ACTGTTTAAA GCAGTACAGT GGAGAAGAGG GCTTTATGAA ACACAACACC 
TCTCGCCAGA ATGAACACTG CCTCACCAAT TTTGACTTGG CTGAGTACCG GCAGGTGCTC 
AGTGACTTGG CAATTCAGAT CTACCAGCAG CTCGTGAGGG TGTTAGAGAA CATCCTTCAG 
CCAATGATCG TCTCCGGAAT GCTGGAGCAT GAAACCATTC AGGGTGTCTC TGGGGTGAAG 
CCCACAGGGC TAAGAAAGCG AACCTCCAGC ATCGCTGACG AGGGTACCTA CACACTGGAC 
TCCATCCTGC GACAGCTCAA CTCCTTCCAC TCGGTCATGT GTCAGCACGG CATGGACCCC 
GAATTGATCA AGCAGGTGGT CAAGCAGATG TTCTACATTG TGGGGGCCGT CACCCTGAAC 
AACCTCCTTT TGCGGAAGGA CATGTGCTCC TGGAGTAAAG GCATGCAGAT CAGGTACAAT 
GTCAGTCAAC TAGAAGAATG GCTGCGTGAC AAGAATTTGA TGAACAGTGG GGCTAAGGAA 
ACCCTGGAGC CTCTCATTCA GGCTGCTCAG CTTTTGCAAG TGAAAAAGAA AACAGATGAT 
GACGCGGAAG CCATTTGTTC CATGTGCAAT GCTTTAACTA CTGCCCAGAT TGTCAAAGTG 
TTGAATTTGT ATACACCAGT TAATGAGTTT GAAGAAAGAG TTTCTGTGTC ATTTATTCGT 
ACTATACAGA TGCGTTTACG AGACAGGAAA GACTCTCCTC AACTTCTCAT GGATGCAAAA 
CACATCTTTC CTGTCACCTT TCCTTTTAAC CCCTCTTCCC TTGCGCTAGA AACCATCCAG 
ATTCCAGCCA GCCTGGGCCT GGGATTCATA TCACGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015465102.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320588.1 Bos taurus myosin VA (MYO5A), transcript variant X4, mRNA.

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CloneID	OBa23396
Clone ID Related Accession (Same CDS sequence)	XM_005211878.2 , XM_015465102.1
Accession Version	XM_015465102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGTGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCGGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGTA TTTTGAGGAA TTATATGCAG ATGACCCTAA GAAGTATCAA 
TCATATCGGA TTTCACTTTA CAAACGGATG ATTGATTTGA TGGAACAACT CGAAAAACAA 
GATAAGACTG TCCGGAAACT GAAAAAACAA CTGAAAGTAT TTGCCAAAAA AATTGGTGAA 
CTAGAAGTGG GCCAGATGGA GAACATATCG CCAGGACAAA TCATTGATGA ACCCATTCGT 
CCAGTCAACA TTCCCAGGAA GGAAAAGGAT TTCCAAGGAA TGCTGGAATA CAAGAAGGAG 
GATGAGCAAA AACTTGTGAA GAACCTGATT CTGGAACTGA AGCCACGTGG TGTAGCAGTC 
AATTTGATTC CAGGGTTACC AGCGTATATC CTGTTTATGT GTGTTCGACA CGCTGACTAC 
CTGAATGATG ATCAGAAAGT AAGGTCGTTG CTAACATCAA CAATTAATAG CATCAAAAAA 
GTATTAAAGA AAAGAGGTGA TGATTTTGAA ACCGTCTCCT TTTGGCTTTC TAACACATGC 
CGATTTTTGC ACTGTTTAAA GCAGTACAGT GGAGAAGAGG GCTTTATGAA ACACAACACC 
TCTCGCCAGA ATGAACACTG CCTCACCAAT TTTGACTTGG CTGAGTACCG GCAGGTGCTC 
AGTGACTTGG CAATTCAGAT CTACCAGCAG CTCGTGAGGG TGTTAGAGAA CATCCTTCAG 
CCAATGATCG TCTCCGGAAT GCTGGAGCAT GAAACCATTC AGGGTGTCTC TGGGGTGAAG 
CCCACAGGGC TAAGAAAGCG AACCTCCAGC ATCGCTGACG AGGGTACCTA CACACTGGAC 
TCCATCCTGC GACAGCTCAA CTCCTTCCAC TCGGTCATGT GTCAGCACGG CATGGACCCC 
GAATTGATCA AGCAGGTGGT CAAGCAGATG TTCTACATTG TGGGGGCCGT CACCCTGAAC 
AACCTCCTTT TGCGGAAGGA CATGTGCTCC TGGAGTAAAG GCATGCAGAT CAGGTACAAT 
GTCAGTCAAC TAGAAGAATG GCTGCGTGAC AAGAATTTGA TGAACAGTGG GGCTAAGGAA 
ACCCTGGAGC CTCTCATTCA GGCTGCTCAG CTTTTGCAAG TGAAAAAGAA AACAGATGAT 
GACGCGGAAG CCATTTGTTC CATGTGCAAT GCTTTAACTA CTGCCCAGAT TGTCAAAGTG 
TTGAATTTGT ATACACCAGT TAATGAGTTT GAAGAAAGAG TTTCTGTGTC ATTTATTCGT 
ACTATACAGA TGCGTTTACG AGACAGGAAA GACTCTCCTC AACTTCTCAT GGATGCAAAA 
CACATCTTTC CTGTCACCTT TCCTTTTAAC CCCTCTTCCC TTGCGCTAGA AACCATCCAG 
ATTCCAGCCA GCCTGGGCCT GGGATTCATA TCACGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320588.1
CDS	137..5698
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015465102.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465102.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGTGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCGGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGTA TTTTGAGGAA TTATATGCAG ATGACCCTAA GAAGTATCAA 
TCATATCGGA TTTCACTTTA CAAACGGATG ATTGATTTGA TGGAACAACT CGAAAAACAA 
GATAAGACTG TCCGGAAACT GAAAAAACAA CTGAAAGTAT TTGCCAAAAA AATTGGTGAA 
CTAGAAGTGG GCCAGATGGA GAACATATCG CCAGGACAAA TCATTGATGA ACCCATTCGT 
CCAGTCAACA TTCCCAGGAA GGAAAAGGAT TTCCAAGGAA TGCTGGAATA CAAGAAGGAG 
GATGAGCAAA AACTTGTGAA GAACCTGATT CTGGAACTGA AGCCACGTGG TGTAGCAGTC 
AATTTGATTC CAGGGTTACC AGCGTATATC CTGTTTATGT GTGTTCGACA CGCTGACTAC 
CTGAATGATG ATCAGAAAGT AAGGTCGTTG CTAACATCAA CAATTAATAG CATCAAAAAA 
GTATTAAAGA AAAGAGGTGA TGATTTTGAA ACCGTCTCCT TTTGGCTTTC TAACACATGC 
CGATTTTTGC ACTGTTTAAA GCAGTACAGT GGAGAAGAGG GCTTTATGAA ACACAACACC 
TCTCGCCAGA ATGAACACTG CCTCACCAAT TTTGACTTGG CTGAGTACCG GCAGGTGCTC 
AGTGACTTGG CAATTCAGAT CTACCAGCAG CTCGTGAGGG TGTTAGAGAA CATCCTTCAG 
CCAATGATCG TCTCCGGAAT GCTGGAGCAT GAAACCATTC AGGGTGTCTC TGGGGTGAAG 
CCCACAGGGC TAAGAAAGCG AACCTCCAGC ATCGCTGACG AGGGTACCTA CACACTGGAC 
TCCATCCTGC GACAGCTCAA CTCCTTCCAC TCGGTCATGT GTCAGCACGG CATGGACCCC 
GAATTGATCA AGCAGGTGGT CAAGCAGATG TTCTACATTG TGGGGGCCGT CACCCTGAAC 
AACCTCCTTT TGCGGAAGGA CATGTGCTCC TGGAGTAAAG GCATGCAGAT CAGGTACAAT 
GTCAGTCAAC TAGAAGAATG GCTGCGTGAC AAGAATTTGA TGAACAGTGG GGCTAAGGAA 
ACCCTGGAGC CTCTCATTCA GGCTGCTCAG CTTTTGCAAG TGAAAAAGAA AACAGATGAT 
GACGCGGAAG CCATTTGTTC CATGTGCAAT GCTTTAACTA CTGCCCAGAT TGTCAAAGTG 
TTGAATTTGT ATACACCAGT TAATGAGTTT GAAGAAAGAG TTTCTGTGTC ATTTATTCGT 
ACTATACAGA TGCGTTTACG AGACAGGAAA GACTCTCCTC AACTTCTCAT GGATGCAAAA 
CACATCTTTC CTGTCACCTT TCCTTTTAAC CCCTCTTCCC TTGCGCTAGA AACCATCCAG 
ATTCCAGCCA GCCTGGGCCT GGGATTCATA TCACGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_005211879.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211936.1 Bos taurus myosin VA (MYO5A), transcript variant X5, mRNA.

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CloneID	OBa23397
Clone ID Related Accession (Same CDS sequence)	XM_005211879.2 , XM_015465103.1
Accession Version	XM_005211879.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005211879.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ACCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGTGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCG GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG GATTTGATGG AACAACTCGA AAAACAAGAT 
AAGACTGTCC GGAAACTGAA AAAACAACTG AAAGTATTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGCC AGATGGAGAA CATATCGCCA GGACAAATCA TTGATGAACC CATTCGTCCA 
GTCAACATTC CCAGGAAGGA AAAGGATTTC CAAGGAATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTGAAGAA CCTGATTCTG GAACTGAAGC CACGTGGTGT AGCAGTCAAT 
TTGATTCCAG GGTTACCAGC GTATATCCTG TTTATGTGTG TTCGACACGC TGACTACCTG 
AATGATGATC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAATAGCAT CAAAAAAGTA 
TTAAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTTT GGCTTTCTAA CACATGCCGA 
TTTTTGCACT GTTTAAAGCA GTACAGTGGA GAAGAGGGCT TTATGAAACA CAACACCTCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACTTGGCTG AGTACCGGCA GGTGCTCAGT 
GACTTGGCAA TTCAGATCTA CCAGCAGCTC GTGAGGGTGT TAGAGAACAT CCTTCAGCCA 
ATGATCGTCT CCGGAATGCT GGAGCATGAA ACCATTCAGG GTGTCTCTGG GGTGAAGCCC 
ACAGGGCTAA GAAAGCGAAC CTCCAGCATC GCTGACGAGG GTACCTACAC ACTGGACTCC 
ATCCTGCGAC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCACGGCAT GGACCCCGAA 
TTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATTGTGG GGGCCGTCAC CCTGAACAAC 
CTCCTTTTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTAG AAGAATGGCT GCGTGACAAG AATTTGATGA ACAGTGGGGC TAAGGAAACC 
CTGGAGCCTC TCATTCAGGC TGCTCAGCTT TTGCAAGTGA AAAAGAAAAC AGATGATGAC 
GCGGAAGCCA TTTGTTCCAT GTGCAATGCT TTAACTACTG CCCAGATTGT CAAAGTGTTG 
AATTTGTATA CACCAGTTAA TGAGTTTGAA GAAAGAGTTT CTGTGTCATT TATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCTCAAC TTCTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACCTTTCC TTTTAACCCC TCTTCCCTTG CGCTAGAAAC CATCCAGATT 
CCAGCCAGCC TGGGCCTGGG ATTCATATCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211936.1
CDS	137..5695
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_005211879.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211879.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ACCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGTGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCG GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG GATTTGATGG AACAACTCGA AAAACAAGAT 
AAGACTGTCC GGAAACTGAA AAAACAACTG AAAGTATTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGCC AGATGGAGAA CATATCGCCA GGACAAATCA TTGATGAACC CATTCGTCCA 
GTCAACATTC CCAGGAAGGA AAAGGATTTC CAAGGAATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTGAAGAA CCTGATTCTG GAACTGAAGC CACGTGGTGT AGCAGTCAAT 
TTGATTCCAG GGTTACCAGC GTATATCCTG TTTATGTGTG TTCGACACGC TGACTACCTG 
AATGATGATC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAATAGCAT CAAAAAAGTA 
TTAAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTTT GGCTTTCTAA CACATGCCGA 
TTTTTGCACT GTTTAAAGCA GTACAGTGGA GAAGAGGGCT TTATGAAACA CAACACCTCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACTTGGCTG AGTACCGGCA GGTGCTCAGT 
GACTTGGCAA TTCAGATCTA CCAGCAGCTC GTGAGGGTGT TAGAGAACAT CCTTCAGCCA 
ATGATCGTCT CCGGAATGCT GGAGCATGAA ACCATTCAGG GTGTCTCTGG GGTGAAGCCC 
ACAGGGCTAA GAAAGCGAAC CTCCAGCATC GCTGACGAGG GTACCTACAC ACTGGACTCC 
ATCCTGCGAC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCACGGCAT GGACCCCGAA 
TTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATTGTGG GGGCCGTCAC CCTGAACAAC 
CTCCTTTTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTAG AAGAATGGCT GCGTGACAAG AATTTGATGA ACAGTGGGGC TAAGGAAACC 
CTGGAGCCTC TCATTCAGGC TGCTCAGCTT TTGCAAGTGA AAAAGAAAAC AGATGATGAC 
GCGGAAGCCA TTTGTTCCAT GTGCAATGCT TTAACTACTG CCCAGATTGT CAAAGTGTTG 
AATTTGTATA CACCAGTTAA TGAGTTTGAA GAAAGAGTTT CTGTGTCATT TATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCTCAAC TTCTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACCTTTCC TTTTAACCCC TCTTCCCTTG CGCTAGAAAC CATCCAGATT 
CCAGCCAGCC TGGGCCTGGG ATTCATATCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015465103.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320589.1 Bos taurus myosin VA (MYO5A), transcript variant X5, mRNA.

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CloneID	OBa23397
Clone ID Related Accession (Same CDS sequence)	XM_005211879.2 , XM_015465103.1
Accession Version	XM_015465103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ACCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGTGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCG GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG GATTTGATGG AACAACTCGA AAAACAAGAT 
AAGACTGTCC GGAAACTGAA AAAACAACTG AAAGTATTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGCC AGATGGAGAA CATATCGCCA GGACAAATCA TTGATGAACC CATTCGTCCA 
GTCAACATTC CCAGGAAGGA AAAGGATTTC CAAGGAATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTGAAGAA CCTGATTCTG GAACTGAAGC CACGTGGTGT AGCAGTCAAT 
TTGATTCCAG GGTTACCAGC GTATATCCTG TTTATGTGTG TTCGACACGC TGACTACCTG 
AATGATGATC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAATAGCAT CAAAAAAGTA 
TTAAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTTT GGCTTTCTAA CACATGCCGA 
TTTTTGCACT GTTTAAAGCA GTACAGTGGA GAAGAGGGCT TTATGAAACA CAACACCTCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACTTGGCTG AGTACCGGCA GGTGCTCAGT 
GACTTGGCAA TTCAGATCTA CCAGCAGCTC GTGAGGGTGT TAGAGAACAT CCTTCAGCCA 
ATGATCGTCT CCGGAATGCT GGAGCATGAA ACCATTCAGG GTGTCTCTGG GGTGAAGCCC 
ACAGGGCTAA GAAAGCGAAC CTCCAGCATC GCTGACGAGG GTACCTACAC ACTGGACTCC 
ATCCTGCGAC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCACGGCAT GGACCCCGAA 
TTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATTGTGG GGGCCGTCAC CCTGAACAAC 
CTCCTTTTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTAG AAGAATGGCT GCGTGACAAG AATTTGATGA ACAGTGGGGC TAAGGAAACC 
CTGGAGCCTC TCATTCAGGC TGCTCAGCTT TTGCAAGTGA AAAAGAAAAC AGATGATGAC 
GCGGAAGCCA TTTGTTCCAT GTGCAATGCT TTAACTACTG CCCAGATTGT CAAAGTGTTG 
AATTTGTATA CACCAGTTAA TGAGTTTGAA GAAAGAGTTT CTGTGTCATT TATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCTCAAC TTCTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACCTTTCC TTTTAACCCC TCTTCCCTTG CGCTAGAAAC CATCCAGATT 
CCAGCCAGCC TGGGCCTGGG ATTCATATCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320589.1
CDS	137..5695
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRPSAMDCHELNEDGELLLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015465103.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465103.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGA ACACAATGAC AGATTCCACA ATACTTTTAG AAGATGTACA GAAAATGAAA 
GATAAAGGCG AAATAGCACA AGCATATATT GGTTTGAAAG AAACCAACAG ACCATCTGCT 
ATGGATTGCC ATGAGTTGAA TGAGGATGGA GAGCTGTTGC TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCCTGGA GTCCCAGCTG CAGTCTCAGA AGAGGAGCCA TGAGAATGAG 
GCTGAAGCTC TCCGTGGGGA AATCCAGAGC CTGAAGGAGG AGAACAACCG GCAGCAGCAG 
CTGCTGGCCC AGAACCTACA GCTGCCCCCG GAGGCCCGCA TTGAGGCCAG CCTGCAGCAC 
GAGATCACTC GGCTCACCAA TGAGAACCTG GATTTGATGG AACAACTCGA AAAACAAGAT 
AAGACTGTCC GGAAACTGAA AAAACAACTG AAAGTATTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGCC AGATGGAGAA CATATCGCCA GGACAAATCA TTGATGAACC CATTCGTCCA 
GTCAACATTC CCAGGAAGGA AAAGGATTTC CAAGGAATGC TGGAATACAA GAAGGAGGAT 
GAGCAAAAAC TTGTGAAGAA CCTGATTCTG GAACTGAAGC CACGTGGTGT AGCAGTCAAT 
TTGATTCCAG GGTTACCAGC GTATATCCTG TTTATGTGTG TTCGACACGC TGACTACCTG 
AATGATGATC AGAAAGTAAG GTCGTTGCTA ACATCAACAA TTAATAGCAT CAAAAAAGTA 
TTAAAGAAAA GAGGTGATGA TTTTGAAACC GTCTCCTTTT GGCTTTCTAA CACATGCCGA 
TTTTTGCACT GTTTAAAGCA GTACAGTGGA GAAGAGGGCT TTATGAAACA CAACACCTCT 
CGCCAGAATG AACACTGCCT CACCAATTTT GACTTGGCTG AGTACCGGCA GGTGCTCAGT 
GACTTGGCAA TTCAGATCTA CCAGCAGCTC GTGAGGGTGT TAGAGAACAT CCTTCAGCCA 
ATGATCGTCT CCGGAATGCT GGAGCATGAA ACCATTCAGG GTGTCTCTGG GGTGAAGCCC 
ACAGGGCTAA GAAAGCGAAC CTCCAGCATC GCTGACGAGG GTACCTACAC ACTGGACTCC 
ATCCTGCGAC AGCTCAACTC CTTCCACTCG GTCATGTGTC AGCACGGCAT GGACCCCGAA 
TTGATCAAGC AGGTGGTCAA GCAGATGTTC TACATTGTGG GGGCCGTCAC CCTGAACAAC 
CTCCTTTTGC GGAAGGACAT GTGCTCCTGG AGTAAAGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTAG AAGAATGGCT GCGTGACAAG AATTTGATGA ACAGTGGGGC TAAGGAAACC 
CTGGAGCCTC TCATTCAGGC TGCTCAGCTT TTGCAAGTGA AAAAGAAAAC AGATGATGAC 
GCGGAAGCCA TTTGTTCCAT GTGCAATGCT TTAACTACTG CCCAGATTGT CAAAGTGTTG 
AATTTGTATA CACCAGTTAA TGAGTTTGAA GAAAGAGTTT CTGTGTCATT TATTCGTACT 
ATACAGATGC GTTTACGAGA CAGGAAAGAC TCTCCTCAAC TTCTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACCTTTCC TTTTAACCCC TCTTCCCTTG CGCTAGAAAC CATCCAGATT 
CCAGCCAGCC TGGGCCTGGG ATTCATATCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_005211880.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211937.1 Bos taurus myosin VA (MYO5A), transcript variant X6, mRNA.

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CloneID	OBa23398
Clone ID Related Accession (Same CDS sequence)	XM_005211880.2 , XM_015465104.1
Accession Version	XM_005211880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005211880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGTGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCGGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGGA TTTGATGGAA CAACTCGAAA AACAAGATAA GACTGTCCGG 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GTGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCGCCAGG ACAAATCATT GATGAACCCA TTCGTCCAGT CAACATTCCC 
AGGAAGGAAA AGGATTTCCA AGGAATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTGAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGG 
TTACCAGCGT ATATCCTGTT TATGTGTGTT CGACACGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AATAGCATCA AAAAAGTATT AAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTTTGG CTTTCTAACA CATGCCGATT TTTGCACTGT 
TTAAAGCAGT ACAGTGGAGA AGAGGGCTTT ATGAAACACA ACACCTCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CTTGGCTGAG TACCGGCAGG TGCTCAGTGA CTTGGCAATT 
CAGATCTACC AGCAGCTCGT GAGGGTGTTA GAGAACATCC TTCAGCCAAT GATCGTCTCC 
GGAATGCTGG AGCATGAAAC CATTCAGGGT GTCTCTGGGG TGAAGCCCAC AGGGCTAAGA 
AAGCGAACCT CCAGCATCGC TGACGAGGGT ACCTACACAC TGGACTCCAT CCTGCGACAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CACGGCATGG ACCCCGAATT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATTGTGGGG GCCGTCACCC TGAACAACCT CCTTTTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTAGAA 
GAATGGCTGC GTGACAAGAA TTTGATGAAC AGTGGGGCTA AGGAAACCCT GGAGCCTCTC 
ATTCAGGCTG CTCAGCTTTT GCAAGTGAAA AAGAAAACAG ATGATGACGC GGAAGCCATT 
TGTTCCATGT GCAATGCTTT AACTACTGCC CAGATTGTCA AAGTGTTGAA TTTGTATACA 
CCAGTTAATG AGTTTGAAGA AAGAGTTTCT GTGTCATTTA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCTCAACTT CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACCTTTCCTT TTAACCCCTC TTCCCTTGCG CTAGAAACCA TCCAGATTCC AGCCAGCCTG 
GGCCTGGGAT TCATATCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211937.1
CDS	137..5623
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_005211880.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211880.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGTGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCGGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGGA TTTGATGGAA CAACTCGAAA AACAAGATAA GACTGTCCGG 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GTGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCGCCAGG ACAAATCATT GATGAACCCA TTCGTCCAGT CAACATTCCC 
AGGAAGGAAA AGGATTTCCA AGGAATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTGAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGG 
TTACCAGCGT ATATCCTGTT TATGTGTGTT CGACACGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AATAGCATCA AAAAAGTATT AAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTTTGG CTTTCTAACA CATGCCGATT TTTGCACTGT 
TTAAAGCAGT ACAGTGGAGA AGAGGGCTTT ATGAAACACA ACACCTCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CTTGGCTGAG TACCGGCAGG TGCTCAGTGA CTTGGCAATT 
CAGATCTACC AGCAGCTCGT GAGGGTGTTA GAGAACATCC TTCAGCCAAT GATCGTCTCC 
GGAATGCTGG AGCATGAAAC CATTCAGGGT GTCTCTGGGG TGAAGCCCAC AGGGCTAAGA 
AAGCGAACCT CCAGCATCGC TGACGAGGGT ACCTACACAC TGGACTCCAT CCTGCGACAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CACGGCATGG ACCCCGAATT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATTGTGGGG GCCGTCACCC TGAACAACCT CCTTTTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTAGAA 
GAATGGCTGC GTGACAAGAA TTTGATGAAC AGTGGGGCTA AGGAAACCCT GGAGCCTCTC 
ATTCAGGCTG CTCAGCTTTT GCAAGTGAAA AAGAAAACAG ATGATGACGC GGAAGCCATT 
TGTTCCATGT GCAATGCTTT AACTACTGCC CAGATTGTCA AAGTGTTGAA TTTGTATACA 
CCAGTTAATG AGTTTGAAGA AAGAGTTTCT GTGTCATTTA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCTCAACTT CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACCTTTCCTT TTAACCCCTC TTCCCTTGCG CTAGAAACCA TCCAGATTCC AGCCAGCCTG 
GGCCTGGGAT TCATATCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5A ( XM_015465104.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320590.1 Bos taurus myosin VA (MYO5A), transcript variant X6, mRNA.

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CloneID	OBa23398
Clone ID Related Accession (Same CDS sequence)	XM_005211880.2 , XM_015465104.1
Accession Version	XM_015465104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	unconventional myosin-Va isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGTGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCGGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGGA TTTGATGGAA CAACTCGAAA AACAAGATAA GACTGTCCGG 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GTGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCGCCAGG ACAAATCATT GATGAACCCA TTCGTCCAGT CAACATTCCC 
AGGAAGGAAA AGGATTTCCA AGGAATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTGAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGG 
TTACCAGCGT ATATCCTGTT TATGTGTGTT CGACACGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AATAGCATCA AAAAAGTATT AAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTTTGG CTTTCTAACA CATGCCGATT TTTGCACTGT 
TTAAAGCAGT ACAGTGGAGA AGAGGGCTTT ATGAAACACA ACACCTCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CTTGGCTGAG TACCGGCAGG TGCTCAGTGA CTTGGCAATT 
CAGATCTACC AGCAGCTCGT GAGGGTGTTA GAGAACATCC TTCAGCCAAT GATCGTCTCC 
GGAATGCTGG AGCATGAAAC CATTCAGGGT GTCTCTGGGG TGAAGCCCAC AGGGCTAAGA 
AAGCGAACCT CCAGCATCGC TGACGAGGGT ACCTACACAC TGGACTCCAT CCTGCGACAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CACGGCATGG ACCCCGAATT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATTGTGGGG GCCGTCACCC TGAACAACCT CCTTTTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTAGAA 
GAATGGCTGC GTGACAAGAA TTTGATGAAC AGTGGGGCTA AGGAAACCCT GGAGCCTCTC 
ATTCAGGCTG CTCAGCTTTT GCAAGTGAAA AAGAAAACAG ATGATGACGC GGAAGCCATT 
TGTTCCATGT GCAATGCTTT AACTACTGCC CAGATTGTCA AAGTGTTGAA TTTGTATACA 
CCAGTTAATG AGTTTGAAGA AAGAGTTTCT GTGTCATTTA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCTCAACTT CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACCTTTCCTT TTAACCCCTC TTCCCTTGCG CTAGAAACCA TCCAGATTCC AGCCAGCCTG 
GGCCTGGGAT TCATATCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320590.1
CDS	137..5623
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLQLEDGKDLEYRLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASADLSEFKVLRLGDANNFHYTNQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVVFMSRDSDSCTIPPKHEPLSIFCDLMGVDFEELCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTLSKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLIVDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITVQRYVRGHQARCYAKFLRRTKAATIIQKYWRMYVARRRYKIMRTATIVLQSYLRGYLARNRYHKILREHKAVIIQKWVRGWLARTCYRRSIHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKIATGRVLSLQEEIAKLRKDLEQTQSEKKSIEEHADRYKQETEQLVSNLKEENTLLKQEKEVLNHRIVEQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVNVPKPGHKRTDSTHSSNESEYTFSSEIAETEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	XM_015465104.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin VA (MYO5A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465104.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACAAAGTTT GCCAGGGTTT GGATACCTGA TCCTGAAGAA 
GTTTGGAAGT CAGCAGAGCT GCTCAAAGAT TATAAGCCAG GAGATAAAGT TCTCCTGCTT 
CAACTTGAGG ATGGAAAGGA TTTGGAATAT CGTCTAGATC CAAAGACCAA GGAACTGCCT 
CATTTAAGAA ACCCTGACAT ACTTGTGGGT GAAAATGATC TCACAGCCCT CAGTTATCTT 
CACGAGCCTG CTGTACTCCA TAATCTCAGA GTCCGCTTTA TTGATTCCAA ACTTATTTAT 
ACATATTGTG GTATAGTCCT AGTAGCTATA AATCCTTATG AACAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCATA TAGTGGTCAG AACATGGGTG ATATGGATCC ACATATCTTT 
GCAGTAGCTG AAGAAGCTTA CAAGCAAATG GCCAGAGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCTGGGGC AGGAAAAACA GTTTCAGCTA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAGCC AATGTTGAGG AAAAGGTGTT GGCTTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCTAAA ACAACCAGGA ATGATAATAG TAGCCGTTTT 
GGGAAGTACA TTGAGATTGG TTTTGATAAG AGATATCGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTATCAT 
ATCTTCTATC AACTTTGTGC CTCAGCAGAC TTATCTGAAT TTAAAGTGCT GCGATTAGGA 
GATGCAAATA ACTTTCATTA CACGAATCAA GGTGGCAGTC CTGTGATTGA AGGAGTAGAT 
GATGCCAAGG AGATGGCACA TACCAGGCAG GCCTGCACTT TGCTAGGAAT TAGTGAATCT 
CATCAGATGG GAATTTTCCG AATACTTGCT GGCATCCTAC ACTTAGGCAA TGTTGTGTTT 
ATGTCTCGAG ATTCAGACAG CTGCACAATC CCTCCCAAGC ATGAACCCCT CAGCATCTTC 
TGTGACCTCA TGGGTGTGGA CTTTGAGGAG CTGTGTCACT GGCTCTGCCA TCGGAAGTTG 
GCCACTGCCA CAGAGACGTA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC GAATGCCCGT 
GATGCATTGG CCAAGCACAT CTATGCCAAG CTCTTTAACT GGATTGTAGA TCATGTCAAT 
CAGGCACTCC ATTCTGCTGT CAAACAGCAT TCTTTTATCG GTGTACTGGA CATTTATGGA 
TTTGAAACAT TTGAGATAAA TAGTTTTGAA CAGTTTTGCA TAAATTATGC AAATGAAAAA 
CTACAGCAAC AATTCAATAT GCACGTTTTC AAATTAGAAC AAGAAGAATA TATGAAAGAA 
CAAATTCCAT GGACACTCAT AGATTTTTAT GATAATCAGC CTTGCATTAA TCTTATAGAA 
TCTAAACTGG GCATTCTAGA TTTGCTGGAT GAGGAATGCA AGATGCCTAA AGGTACAGAT 
GACACTTGGG CTCAAAAATT GTACAACACA CATTTGAACA AATGTGCACT ATTTGAGAAG 
CCCCGCCTAT CAAACAAAGC TTTCATCATC CAGCATTTTG CTGACAAAGT GGAATACCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAAGAC ACCGTTTTTG AAGAACAAAT TAAAGTTCTT 
AAATCAAGCA AGTTTAAGAT GCTACCAGAA TTATTTCAAG ACGATGAGAA GGCCATCAGC 
CCAACCTCAG CCACCTCTTC AGGGCGCACG CCTCTCACCC GCACCCTTTC AAAGCCCACC 
AAAGGCAGAC CAGGCCAGAC GGCCAAAGAG CACAAGAAAA CAGTGGGGCA CCAGTTCCGA 
AACTCCCTCC ACCTGCTTAT GGAGACCCTG AATGCCACTA CCCCTCACTA TGTGCGCTGT 
ATCAAGCCTA ACGACTTCAA GTTCCCATTC ACGTTTGATG AGAAGAGGGC AGTGCAGCAG 
CTGAGAGCAT GCGGTGTCCT GGAAACCATT CGGATCAGCG CAGCAGGTTT CCCCTCTCGG 
TGGACTTACC AAGAATTTTT CAGCCGCTAT CGGGTCCTAA TGAAGCAAAA AGATGTGCTA 
AGTGACAGAA AGCAAACATG CAAGAATGTT TTAGAGAAAC TGATAGTGGA CAAGGACAAA 
TACCAGTTTG GGAAGACAAA GATCTTTTTC CGGGCTGGTC AAGTGGCCTA CCTAGAAAAA 
TTGAGGGCAG ACAAGCTGAG GGCTGCCTGC ATCCGGATCC AGAAGACGAT CCGAGGGTGG 
CTGCTGCGGA AGAAGTACCT GCGCATGCGC AAGGCTGCCA TCACTGTGCA GAGATATGTG 
CGAGGCCACC AGGCCCGATG CTATGCTAAG TTCCTGCGCA GAACCAAGGC AGCCACTATC 
ATTCAGAAGT ACTGGCGCAT GTATGTAGCT CGCCGGAGAT ACAAGATCAT GCGAACTGCC 
ACAATTGTCC TTCAGTCTTA TTTGCGAGGC TACTTGGCCA GAAATAGGTA TCACAAGATA 
CTCCGTGAAC ACAAAGCAGT CATCATTCAG AAATGGGTCC GTGGCTGGCT GGCTCGCACC 
TGCTATAGGA GGAGCATCCA CGCCATCATC TACCTTCAGT GCTGCTTCCG ACGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTTAAGATC GAGGCCCGCT CTGTGGAGCG CTATAAGAAG 
CTCCACATTG GCATGGAGAA CAAGATCATG CAGCTGCAGC GCAAAGTTGA TGAGCAGAAC 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTAG AAGGAATATA CAACTCGGAG 
ACCGAGAAAC TACGAAGTGA TTTAGAACGT CTTCAGCTAA GTGAAGAAGA AGCTAAGATT 
GCTACTGGAC GGGTCCTCAG TCTGCAGGAA GAAATTGCCA AGCTACGGAA AGACCTGGAA 
CAAACTCAGT CTGAGAAAAA ATCCATTGAG GAACATGCTG ATAGATACAA ACAAGAAACT 
GAGCAGCTGG TGTCAAATCT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAAGAGGTC 
CTCAATCACC GTATCGTGGA GCAGGCTAAG GAGATGACAG AGACTATGGA GAAGAAGTTA 
GTAGAAGAAA CAAAACAACT GGAACTCGAT CTTAACGATG AAAGGCTGAG ATATCAGAAC 
CTTCTGAATG AGTTTAGTCG CTTGGAAGAA CGCTATGATG ACCTCAAGGA AGAGATGACT 
TTGATGGTGA ATGTGCCTAA ACCTGGACAC AAGAGAACAG ACTCTACCCA CAGCAGCAAT 
GAGTCTGAAT ACACCTTTAG CTCTGAAATT GCAGAAACTG AAGATATTCC ATCAAGGACA 
GAGGAGCCAA GTGAGAAGAA GGTGCCTCTG GACATGTCAT TGTTCCTCAA GCTCCAGAAG 
CGGGTCACAG AGCTGGAGCA GGAGAAGCAG GTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGC TCCGCAGCAA GGCAAAGGAA GAAGAAAGAC CACAGATTAG AGGTGCAGAA 
CTGGAATATG AGTCACTCAA GCGTCAAGAA CTAGAGTCAG AAAACAAAAA ACTGAAAAAT 
GAGCTAAATG AGTTACGAAA GGCCCTCAGT GAGAAAAGTG CCCCTGAGGT GACTGCCCCA 
GGCGCGCCAG CCTACCGTGT CCTCATGGAG CAGCTGACGT CTGTGAGTGA GGAGCTTGAT 
GTCCGCAAGG AGGAAGTCCT CATCCTGAGG TCTCAGCTGG TGAGCCAGAA GGAAGCCATA 
CAACCCAAGG ATGACAAGAA CACAATGACA GATTCCACAA TACTTTTAGA AGATGTACAG 
AAAATGAAAG ATAAAGGCGA AATAGCACAA GCATATATTG GTTTGAAAGA AACCAACAGG 
CTCCTGGAGT CCCAGCTGCA GTCTCAGAAG AGGAGCCATG AGAATGAGGC TGAAGCTCTC 
CGTGGGGAAA TCCAGAGCCT GAAGGAGGAG AACAACCGGC AGCAGCAGCT GCTGGCCCAG 
AACCTACAGC TGCCCCCGGA GGCCCGCATT GAGGCCAGCC TGCAGCACGA GATCACTCGG 
CTCACCAATG AGAACCTGGA TTTGATGGAA CAACTCGAAA AACAAGATAA GACTGTCCGG 
AAACTGAAAA AACAACTGAA AGTATTTGCC AAAAAAATTG GTGAACTAGA AGTGGGCCAG 
ATGGAGAACA TATCGCCAGG ACAAATCATT GATGAACCCA TTCGTCCAGT CAACATTCCC 
AGGAAGGAAA AGGATTTCCA AGGAATGCTG GAATACAAGA AGGAGGATGA GCAAAAACTT 
GTGAAGAACC TGATTCTGGA ACTGAAGCCA CGTGGTGTAG CAGTCAATTT GATTCCAGGG 
TTACCAGCGT ATATCCTGTT TATGTGTGTT CGACACGCTG ACTACCTGAA TGATGATCAG 
AAAGTAAGGT CGTTGCTAAC ATCAACAATT AATAGCATCA AAAAAGTATT AAAGAAAAGA 
GGTGATGATT TTGAAACCGT CTCCTTTTGG CTTTCTAACA CATGCCGATT TTTGCACTGT 
TTAAAGCAGT ACAGTGGAGA AGAGGGCTTT ATGAAACACA ACACCTCTCG CCAGAATGAA 
CACTGCCTCA CCAATTTTGA CTTGGCTGAG TACCGGCAGG TGCTCAGTGA CTTGGCAATT 
CAGATCTACC AGCAGCTCGT GAGGGTGTTA GAGAACATCC TTCAGCCAAT GATCGTCTCC 
GGAATGCTGG AGCATGAAAC CATTCAGGGT GTCTCTGGGG TGAAGCCCAC AGGGCTAAGA 
AAGCGAACCT CCAGCATCGC TGACGAGGGT ACCTACACAC TGGACTCCAT CCTGCGACAG 
CTCAACTCCT TCCACTCGGT CATGTGTCAG CACGGCATGG ACCCCGAATT GATCAAGCAG 
GTGGTCAAGC AGATGTTCTA CATTGTGGGG GCCGTCACCC TGAACAACCT CCTTTTGCGG 
AAGGACATGT GCTCCTGGAG TAAAGGCATG CAGATCAGGT ACAATGTCAG TCAACTAGAA 
GAATGGCTGC GTGACAAGAA TTTGATGAAC AGTGGGGCTA AGGAAACCCT GGAGCCTCTC 
ATTCAGGCTG CTCAGCTTTT GCAAGTGAAA AAGAAAACAG ATGATGACGC GGAAGCCATT 
TGTTCCATGT GCAATGCTTT AACTACTGCC CAGATTGTCA AAGTGTTGAA TTTGTATACA 
CCAGTTAATG AGTTTGAAGA AAGAGTTTCT GTGTCATTTA TTCGTACTAT ACAGATGCGT 
TTACGAGACA GGAAAGACTC TCCTCAACTT CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACCTTTCCTT TTAACCCCTC TTCCCTTGCG CTAGAAACCA TCCAGATTCC AGCCAGCCTG 
GGCCTGGGAT TCATATCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MYO5A
Entrez Gene ID	541234
Full Name	myosin VA
General protein information	Preferred Names myosin VA Names unconventional myosin-Va myosin VA (heavy chain 12, myoxin)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	MYO5A	XP_001084476.1
Canis lupus familiaris (dog)	MYO5A	XP_005638745.1
Mus musculus (house mouse)	Myo5a	NP_034994.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006479	XP_316519.4
Kluyveromyces lactis	KLLA0A03905g	XP_451171.1
Magnaporthe oryzae (rice blast fungus)	MGG_16213	XP_003709659.1
Arabidopsis thaliana (thale cress)	XIC	NP_172349.2
Oryza sativa (rice)	Os06g0488200	NP_001057670.1
Arabidopsis thaliana (thale cress)	XIE	NP_175858.1
Bos taurus (cattle)	MYO5A	XP_005211933.1
Rattus norvegicus (Norway rat)	Myo5a	NP_071514.1
Gallus gallus (chicken)	MYO5A	NP_990631.1
Neurospora crassa	NCU11354	XP_001728528.1
Xenopus tropicalis (tropical clawed frog)	myo5a	XP_004912752.1
Pan troglodytes (chimpanzee)	MYO5A	XP_510412.2
Danio rerio (zebrafish)	myo5aa	XP_005173731.1
Drosophila melanogaster (fruit fly)	didum	NP_724569.1
Caenorhabditis elegans (roundworm)	hum-2	NP_001023886.1
Saccharomyces cerevisiae (baker's yeast)	MYO2	NP_014971.1
Homo sapiens (human)	MYO5A	NP_000250.3
Eremothecium gossypii	AGOS_ADR354W	NP_984450.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MYO5A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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