didum cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	didum
Entrez Gene ID	35680
Full Name	dilute class unconventional myosin
Synonyms	43CD,CG2146,Didum,DmV,Dmel\CG2146,M5,MYOV,Myo1C,Myo5,MyoV,MyoV43CD,NEST:bs14c05,NMC7,dmM5,myoV,soy
General protein information	Preferred Names dilute class unconventional myosin Names CG2146 gene product from transcript CG2146-RA CG2146-PA CG2146-PB CG2146-PC MyosinV didum-PA didum-PB didum-PC myosin V myosin-5 myosin-V shorty
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 43C7-43D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_165536.3	NP_724570.1	dilute class unconventional myosin, isoform B
NM_057838.4	NP_477186.1	dilute class unconventional myosin, isoform A
NM_165535.2	NP_724569.1	dilute class unconventional myosin, isoform C

Pathways from BioSystems

Homologies

	MYO5A	XP_001084476.1
Canis lupus familiaris (dog)	MYO5A	XP_005638745.1
Mus musculus (house mouse)	Myo5a	NP_034994.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006479	XP_316519.4
Kluyveromyces lactis	KLLA0A03905g	XP_451171.1
Magnaporthe oryzae (rice blast fungus)	MGG_16213	XP_003709659.1
Arabidopsis thaliana (thale cress)	XIC	NP_172349.2
Oryza sativa (rice)	Os06g0488200	NP_001057670.1
Arabidopsis thaliana (thale cress)	XIE	NP_175858.1
Bos taurus (cattle)	MYO5A	XP_005211933.1
Rattus norvegicus (Norway rat)	Myo5a	NP_071514.1
Gallus gallus (chicken)	MYO5A	NP_990631.1
Neurospora crassa	NCU11354	XP_001728528.1
Xenopus tropicalis (tropical clawed frog)	myo5a	XP_004912752.1
Pan troglodytes (chimpanzee)	MYO5A	XP_510412.2
Danio rerio (zebrafish)	myo5aa	XP_005173731.1
Drosophila melanogaster (fruit fly)	didum	NP_724569.1
Caenorhabditis elegans (roundworm)	hum-2	NP_001023886.1
Saccharomyces cerevisiae (baker's yeast)	MYO2	NP_014971.1
Homo sapiens (human)	MYO5A	NP_000250.3
Eremothecium gossypii	AGOS_ADR354W	NP_984450.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Patronin and Shot then act to polarise microtubules along the apical-basal axis to enable apical transport of Rab11 endosomes by the Nuf-Dynein microtubule motor complex. Finally, Rab11 endosomes are transferred to the MyoV (also known as Didum in Drosophila) actin motor to deliver the key microvillar determinant Cadherin 99C to the apical membrane to organise the biogenesis of actin microvilli.
Title: Shot and Patronin polarise microtubules to direct membrane traffic and biogenesis of microvilli in epithelia.

data indicate that myosin V and VI, but not II, play related but distinct roles in regulating microtubule (MT)-based mitochondrial movement: they oppose, rather than complement, protracted MT-based movements and perhaps facilitate organelle docking
Title: Evidence that myosin activity opposes microtubule-based axonal transport of mitochondria.

the mechanical properties of coiled-coil regions of myosin v
Title: Extensibility of the extended tail domain of processive and nonprocessive myosin V molecules.

Data show that didum, encoding the Drosophila actin-based motor Myosin-V, is a new posterior group gene that promotes posterior accumulation of Oskar.
Title: Myosin-V regulates oskar mRNA localization in the Drosophila oocyte.

Myosin V delivers morphogenic secretory traffic along polarized actin filaments of the subcortical terminal web to the exocytic plasma membrane target, the rhabdomere base.
Title: Myosin V, Rab11, and dRip11 direct apical secretion and cellular morphogenesis in developing Drosophila photoreceptors.

The following didum gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the didum cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa12467	NM_165536.3 Latest version!	Drosophila melanogaster dilute class unconventional myosin (didum), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa12465	NM_057838.4 Latest version!	Drosophila melanogaster dilute class unconventional myosin (didum), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa12466	NM_165535.2 Latest version!	Drosophila melanogaster dilute class unconventional myosin (didum), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

didum ( NM_165536.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724570.1 Drosophila melanogaster dilute class unconventional myosin (didum), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12467
Clone ID Related Accession (Same CDS sequence)	NM_165536.3
Accession Version	NM_165536.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dilute class unconventional myosin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165536.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTAGCG AGGAGATGCT ATACGCGCAG GGCGCCAAGA TCTGGGTGCC CCATGCGGAT 
CTGGTGTGGG AGAGCGCCAC CTTGGAGGAG AGCTACCGCA AGGGCGCCGG CTTCTTGAAG 
ATATGTACGG ACTCCGGAAA ACTGAAAGAG GTCAAGCTAA AGGCCGATGG CAGCGATCTG 
CCTCCACTGC GCAATCCGGC CATTCTGGTG GGACAGAACG ACTTGACCAC CCTGTCCTAC 
CTGCATGAGC CGGGGGTGTT GCACAATCTG CGTGTCCGCT TCTGCGAGCG CCAGATTATC 
TACACCTACT GCGGCATCAT TCTGGTGGCC ATCAACCCGT ACGCGGAGAT GCCTCTTTAC 
GGGCCCAGCA TAATCCGAGC GTATCGGGGT CATGCTATGG GTGATCTGGA GCCGCACATC 
TTTGCCCTGG CGGAGGAGGC GTACACGAAA CTGGAGCGCG AGAACTGCAA CCTGAGCATC 
ATCGTCAGTG GGGAGTCGGG TGCGGGCAAG ACGGTGTCCG CCAAATACGC CATGAGGTAC 
TTTGCCGCTG TTGGAGGTTC CGAGTCGGAG ACCCAGGTCG AACGCAAGGT GCTGGCATCT 
TCGCCGATCA TGGAAGCCTT CGGAAATGCC AAGACGACCC GGAATGACAA CAGTTCCCGC 
TTTGGAAAGT TTACCAAGCT GCTGTTCCGG AACCAGATGG GTGTGATGTT CCTGCAGGGA 
GCCACTATGC ACACCTACCT ACTGGAGAAG TCACGTGTGG TGTACCAGGC CCAGGGAGAG 
CGCAACTATC ACATATTCTA TCAGCTGTGC GCGGCGCGAT CGAAGTACCC TGAACTGGTG 
CTGGATCACC AGGACAAATT CCAGTTTCTG AACATGGGTG GCGCTCCTGA AATTGAACGA 
GTTTCGGATG CGGAGCAGTT TAACGAAACC GTGCAGGCCA TGACAGTTCT GGGCTTCTCC 
ATTCAACAGA TCGCTGATAT CGTAAAGATC CTGGCAGGAA TACTCCATTT AGGAAACATT 
CAGGTTTCCA AGAAGTTCAA CGAGGGCAGC GAAGAGGAGG ACAGTGACTC TTGCGATATA 
TTTCATAACG ACATCCACCT GCAGATCACC GCCGATCTAC TGCGGGTGAG CGCCGATGAT 
CTGCGCCGGT GGCTTTTGAT GCGTAAGATA GAGTCGGTCA ATGAATATGT GCTGATACCG 
AATAGCATTG AGGCGGCTCA GGCGGCTCGA GACGCTCTGG CCAAGCACAT CTATGCGAAA 
CTGTTTCAGT ATATAGTCGG TGTGCTGAAC AAGAGCCTCA ACAACGGTAG CAAGCAGTGC 
AGCTTCATTG GCGTCCTCGA TATCTACGGC TTCGAAACGT TCGAGGTGAA CTCCTTTGAA 
CAATTTTGCA TAAACTATGC GAACGAAAAG CTTCAGCAGC AGTTCAACCA GCATGTCTTC 
AAGCTGGAGC AGGAGGAGTA TCTTAAGGAA GGAATCACCT GGACGATGAT TGACTTTTAC 
GACAATCAAC CGTGTATCGA TCTAATTGAA TCTCGCTTGG GAGTGCTGGA CCTGCTCGAC 
GAGGAGTGTC GAATGCCAAA GGGCTCGGAC GAGAGCTGGG CTGGCAAGCT CATCGGAAAG 
TGCAATAAAT TTCCGCATTT CGAGAAGCCA CGCTTTGGCA CAACCAGCTT CTTTATCAAA 
CATTTCTCGG ACACGGTCGA GTATGACGTG AACGGATTCT TGGAAAAGAA TCGTGACACA 
GTCTCCAAGG AGTTGACGCA AGTGCTAAGC GAGTCCAACA TGTCTCTGGC CAAGCAGGTG 
ATGACCCTGG AGGAAATAGA TACTCTGTGC GTGGATTCCG CTAAATCCTC CACCTTGGGC 
GGCCGCGTCG TGATCAGTGC TGGCCGCAAA CAGGTGGTGC CATCCAAGCA GCATAGAAAA 
ACGGTGGGAT CGCAGTTCCA GGAGAGTCTG GCGTCGCTGA TATCTACGTT ACATGCCACA 
ACTCCGCACT ATGTGCGCTG CATCAAGCCC AACGATGACA AAGTCGCCTT TAAGTGGGAG 
ACGGCCAAGA TCATACAGCA GTTAAGGGCC TGTGGTGTGC TGGAAACGGT GCGCATCTCC 
GCAGCGGGAT TCCCCTCGAG ATGGCTCTAT CCCGACTTCT ATATGCGGTA CCAGCTGCTG 
GTTTACCGCT CCAAACTCGA CAAAAACGAC ATGAAGCTGT CGTGCCGGAA CATTGTGATG 
AAGTGGATCC AAGACGAAGA TAAGTACCGA TTTGGCAACA CGCAGATTTT CTTCCGCGCC 
GGCCAAGTGG CCTTCCTTGA ACAGGTTCGG GCTAATCTGC GCAAGAAGTA TATCACCATT 
GTGCAGTCGG TTGTGCGGCG ATTCGTCTAC CGGCGCCAGT TCCTGCGCAT TCAGAAAGTA 
ATTAATGGCA TTCAGAAACA TGCGCGCGGA TATCTTGCTC GCGAGCGTAC TCAGAAAATG 
CGCGAAGCTC GTGCGGGATT AATCCTGTCG AAGTACGCCC GCGGTTGGTT ATGCCGTCGT 
CGTTACTTGC GCCTACGCCA CTCCATTTCC GGCATACAGA CCTACGCCCG CGGCATGCTG 
GCGCGCAACA AGTTCCACGC GATGCGGGAT CACTACCGGG CAGTTCAGAT CCAGCGTTTC 
GTGCGTGGTG CTTTGGCACG GCGAGCTTAC CAAAAGCGGC GTCGCAACAT CATCATTTGT 
CAAGCGGCGA TTCGGAGATT CTTGGCCCGT CGTAAGTTTA AACGCATGAA GGCCGAGGCC 
AAGACCATCT CGCACATGGA AAACAAATAC ATGGGGCTGG AAAACAAGAT TATTTCCATG 
CAGCAGCGGA TCGATGAGCT GAATCGCGAC AACAGTAATC TGAAGCACAA GACCAGCGAA 
ATCAGTGTAT TGAAAATGAA GCTTGAGCTG AAGAAGACCC TGGAGGCTGA ATTCAAAAAT 
GTCAAGGCCG CCTGCCAGGA CAAGGACAAG CTGATCGAAG CACTTAACAA GCAGTTGGAG 
GCGGAGCGAG ACGAAAAAAT GCAGTTGCTG GAGGAGAACG GACATGCTCA AGAGGAGTGG 
ATCAGCCAGA AGCAGACGTG GCGCCAAGAG AACGAGGAGC TGCGCCGTCA GATAGACGAG 
ATAATCGATA TGGCAAAGAA CGCAGAAGTC AACCAGCGTA ACCAGGAGGA CCGAATGCTA 
GCCGAGATTG ATAACAGGGA GCTCAACGAG GCCTACCAAC GAGCTATTAA GGACAAGGAG 
GTCATCGAGA ACGAAAACTT CATGCTGAAG GAAGAGCTCA GTCGATTAAC GGCTGGCAGT 
TTCAGTTTGC ACGCCCGCAA GGCTAGCAAC GCCTCCAGCC AAAACGAGGA CGATGTGGGA 
TACGCCTCCG CCAAGAACAC TCTGGATATC AATCGGCCCC CGGATTTGTT AAGCAAAAAT 
TACTCGTACA ATGACTCTAC CAGTCTGGTG GTGAAGTTGA GATCCATTCT CGAGGAGGAG 
AAGCAAAAGC ACAAGGTCTT GCAGGAGCAG TACATTAAGT TGTCCAGTCG GCATAAGCCC 
ACCGAGGATT CCTTCCGCAC AGCATGCCGC CTTGCAGGAA GAGGTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724570.1
CDS	333..3923
Translation	MSSEEMLYAQGAKIWVPHADLVWESATLEESYRKGAGFLKICTDSGKLKEVKLKADGSDLPPLRNPAILVGQNDLTTLSYLHEPGVLHNLRVRFCERQIIYTYCGIILVAINPYAEMPLYGPSIIRAYRGHAMGDLEPHIFALAEEAYTKLERENCNLSIIVSGESGAGKTVSAKYAMRYFAAVGGSESETQVERKVLASSPIMEAFGNAKTTRNDNSSRFGKFTKLLFRNQMGVMFLQGATMHTYLLEKSRVVYQAQGERNYHIFYQLCAARSKYPELVLDHQDKFQFLNMGGAPEIERVSDAEQFNETVQAMTVLGFSIQQIADIVKILAGILHLGNIQVSKKFNEGSEEEDSDSCDIFHNDIHLQITADLLRVSADDLRRWLLMRKIESVNEYVLIPNSIEAAQAARDALAKHIYAKLFQYIVGVLNKSLNNGSKQCSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNQHVFKLEQEEYLKEGITWTMIDFYDNQPCIDLIESRLGVLDLLDEECRMPKGSDESWAGKLIGKCNKFPHFEKPRFGTTSFFIKHFSDTVEYDVNGFLEKNRDTVSKELTQVLSESNMSLAKQVMTLEEIDTLCVDSAKSSTLGGRVVISAGRKQVVPSKQHRKTVGSQFQESLASLISTLHATTPHYVRCIKPNDDKVAFKWETAKIIQQLRACGVLETVRISAAGFPSRWLYPDFYMRYQLLVYRSKLDKNDMKLSCRNIVMKWIQDEDKYRFGNTQIFFRAGQVAFLEQVRANLRKKYITIVQSVVRRFVYRRQFLRIQKVINGIQKHARGYLARERTQKMREARAGLILSKYARGWLCRRRYLRLRHSISGIQTYARGMLARNKFHAMRDHYRAVQIQRFVRGALARRAYQKRRRNIIICQAAIRRFLARRKFKRMKAEAKTISHMENKYMGLENKIISMQQRIDELNRDNSNLKHKTSEISVLKMKLELKKTLEAEFKNVKAACQDKDKLIEALNKQLEAERDEKMQLLEENGHAQEEWISQKQTWRQENEELRRQIDEIIDMAKNAEVNQRNQEDRMLAEIDNRELNEAYQRAIKDKEVIENENFMLKEELSRLTAGSFSLHARKASNASSQNEDDVGYASAKNTLDINRPPDLLSKNYSYNDSTSLVVKLRSILEEEKQKHKVLQEQYIKLSSRHKPTEDSFRTACRLAGRGT

Target ORF information:

RefSeq Version	NM_165536.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dilute class unconventional myosin (didum), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165536.3

ORF Insert Sequence:

ATGTCTAGCG AGGAGATGCT ATACGCGCAG GGCGCCAAGA TCTGGGTGCC CCATGCGGAT 
CTGGTGTGGG AGAGCGCCAC CTTGGAGGAG AGCTACCGCA AGGGCGCCGG CTTCTTGAAG 
ATATGTACGG ACTCCGGAAA ACTGAAAGAG GTCAAGCTAA AGGCCGATGG CAGCGATCTG 
CCTCCACTGC GCAATCCGGC CATTCTGGTG GGACAGAACG ACTTGACCAC CCTGTCCTAC 
CTGCATGAGC CGGGGGTGTT GCACAATCTG CGTGTCCGCT TCTGCGAGCG CCAGATTATC 
TACACCTACT GCGGCATCAT TCTGGTGGCC ATCAACCCGT ACGCGGAGAT GCCTCTTTAC 
GGGCCCAGCA TAATCCGAGC GTATCGGGGT CATGCTATGG GTGATCTGGA GCCGCACATC 
TTTGCCCTGG CGGAGGAGGC GTACACGAAA CTGGAGCGCG AGAACTGCAA CCTGAGCATC 
ATCGTCAGTG GGGAGTCGGG TGCGGGCAAG ACGGTGTCCG CCAAATACGC CATGAGGTAC 
TTTGCCGCTG TTGGAGGTTC CGAGTCGGAG ACCCAGGTCG AACGCAAGGT GCTGGCATCT 
TCGCCGATCA TGGAAGCCTT CGGAAATGCC AAGACGACCC GGAATGACAA CAGTTCCCGC 
TTTGGAAAGT TTACCAAGCT GCTGTTCCGG AACCAGATGG GTGTGATGTT CCTGCAGGGA 
GCCACTATGC ACACCTACCT ACTGGAGAAG TCACGTGTGG TGTACCAGGC CCAGGGAGAG 
CGCAACTATC ACATATTCTA TCAGCTGTGC GCGGCGCGAT CGAAGTACCC TGAACTGGTG 
CTGGATCACC AGGACAAATT CCAGTTTCTG AACATGGGTG GCGCTCCTGA AATTGAACGA 
GTTTCGGATG CGGAGCAGTT TAACGAAACC GTGCAGGCCA TGACAGTTCT GGGCTTCTCC 
ATTCAACAGA TCGCTGATAT CGTAAAGATC CTGGCAGGAA TACTCCATTT AGGAAACATT 
CAGGTTTCCA AGAAGTTCAA CGAGGGCAGC GAAGAGGAGG ACAGTGACTC TTGCGATATA 
TTTCATAACG ACATCCACCT GCAGATCACC GCCGATCTAC TGCGGGTGAG CGCCGATGAT 
CTGCGCCGGT GGCTTTTGAT GCGTAAGATA GAGTCGGTCA ATGAATATGT GCTGATACCG 
AATAGCATTG AGGCGGCTCA GGCGGCTCGA GACGCTCTGG CCAAGCACAT CTATGCGAAA 
CTGTTTCAGT ATATAGTCGG TGTGCTGAAC AAGAGCCTCA ACAACGGTAG CAAGCAGTGC 
AGCTTCATTG GCGTCCTCGA TATCTACGGC TTCGAAACGT TCGAGGTGAA CTCCTTTGAA 
CAATTTTGCA TAAACTATGC GAACGAAAAG CTTCAGCAGC AGTTCAACCA GCATGTCTTC 
AAGCTGGAGC AGGAGGAGTA TCTTAAGGAA GGAATCACCT GGACGATGAT TGACTTTTAC 
GACAATCAAC CGTGTATCGA TCTAATTGAA TCTCGCTTGG GAGTGCTGGA CCTGCTCGAC 
GAGGAGTGTC GAATGCCAAA GGGCTCGGAC GAGAGCTGGG CTGGCAAGCT CATCGGAAAG 
TGCAATAAAT TTCCGCATTT CGAGAAGCCA CGCTTTGGCA CAACCAGCTT CTTTATCAAA 
CATTTCTCGG ACACGGTCGA GTATGACGTG AACGGATTCT TGGAAAAGAA TCGTGACACA 
GTCTCCAAGG AGTTGACGCA AGTGCTAAGC GAGTCCAACA TGTCTCTGGC CAAGCAGGTG 
ATGACCCTGG AGGAAATAGA TACTCTGTGC GTGGATTCCG CTAAATCCTC CACCTTGGGC 
GGCCGCGTCG TGATCAGTGC TGGCCGCAAA CAGGTGGTGC CATCCAAGCA GCATAGAAAA 
ACGGTGGGAT CGCAGTTCCA GGAGAGTCTG GCGTCGCTGA TATCTACGTT ACATGCCACA 
ACTCCGCACT ATGTGCGCTG CATCAAGCCC AACGATGACA AAGTCGCCTT TAAGTGGGAG 
ACGGCCAAGA TCATACAGCA GTTAAGGGCC TGTGGTGTGC TGGAAACGGT GCGCATCTCC 
GCAGCGGGAT TCCCCTCGAG ATGGCTCTAT CCCGACTTCT ATATGCGGTA CCAGCTGCTG 
GTTTACCGCT CCAAACTCGA CAAAAACGAC ATGAAGCTGT CGTGCCGGAA CATTGTGATG 
AAGTGGATCC AAGACGAAGA TAAGTACCGA TTTGGCAACA CGCAGATTTT CTTCCGCGCC 
GGCCAAGTGG CCTTCCTTGA ACAGGTTCGG GCTAATCTGC GCAAGAAGTA TATCACCATT 
GTGCAGTCGG TTGTGCGGCG ATTCGTCTAC CGGCGCCAGT TCCTGCGCAT TCAGAAAGTA 
ATTAATGGCA TTCAGAAACA TGCGCGCGGA TATCTTGCTC GCGAGCGTAC TCAGAAAATG 
CGCGAAGCTC GTGCGGGATT AATCCTGTCG AAGTACGCCC GCGGTTGGTT ATGCCGTCGT 
CGTTACTTGC GCCTACGCCA CTCCATTTCC GGCATACAGA CCTACGCCCG CGGCATGCTG 
GCGCGCAACA AGTTCCACGC GATGCGGGAT CACTACCGGG CAGTTCAGAT CCAGCGTTTC 
GTGCGTGGTG CTTTGGCACG GCGAGCTTAC CAAAAGCGGC GTCGCAACAT CATCATTTGT 
CAAGCGGCGA TTCGGAGATT CTTGGCCCGT CGTAAGTTTA AACGCATGAA GGCCGAGGCC 
AAGACCATCT CGCACATGGA AAACAAATAC ATGGGGCTGG AAAACAAGAT TATTTCCATG 
CAGCAGCGGA TCGATGAGCT GAATCGCGAC AACAGTAATC TGAAGCACAA GACCAGCGAA 
ATCAGTGTAT TGAAAATGAA GCTTGAGCTG AAGAAGACCC TGGAGGCTGA ATTCAAAAAT 
GTCAAGGCCG CCTGCCAGGA CAAGGACAAG CTGATCGAAG CACTTAACAA GCAGTTGGAG 
GCGGAGCGAG ACGAAAAAAT GCAGTTGCTG GAGGAGAACG GACATGCTCA AGAGGAGTGG 
ATCAGCCAGA AGCAGACGTG GCGCCAAGAG AACGAGGAGC TGCGCCGTCA GATAGACGAG 
ATAATCGATA TGGCAAAGAA CGCAGAAGTC AACCAGCGTA ACCAGGAGGA CCGAATGCTA 
GCCGAGATTG ATAACAGGGA GCTCAACGAG GCCTACCAAC GAGCTATTAA GGACAAGGAG 
GTCATCGAGA ACGAAAACTT CATGCTGAAG GAAGAGCTCA GTCGATTAAC GGCTGGCAGT 
TTCAGTTTGC ACGCCCGCAA GGCTAGCAAC GCCTCCAGCC AAAACGAGGA CGATGTGGGA 
TACGCCTCCG CCAAGAACAC TCTGGATATC AATCGGCCCC CGGATTTGTT AAGCAAAAAT 
TACTCGTACA ATGACTCTAC CAGTCTGGTG GTGAAGTTGA GATCCATTCT CGAGGAGGAG 
AAGCAAAAGC ACAAGGTCTT GCAGGAGCAG TACATTAAGT TGTCCAGTCG GCATAAGCCC 
ACCGAGGATT CCTTCCGCAC AGCATGCCGC CTTGCAGGAA GAGGTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

didum ( NM_057838.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477186.1 Drosophila melanogaster dilute class unconventional myosin (didum), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12465
Clone ID Related Accession (Same CDS sequence)	NM_057838.4
Accession Version	NM_057838.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dilute class unconventional myosin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057838.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTAGCG AGGAGATGCT ATACGCGCAG GGCGCCAAGA TCTGGGTGCC CCATGCGGAT 
CTGGTGTGGG AGAGCGCCAC CTTGGAGGAG AGCTACCGCA AGGGCGCCGG CTTCTTGAAG 
ATATGTACGG ACTCCGGAAA ACTGAAAGAG GTCAAGCTAA AGGCCGATGG CAGCGATCTG 
CCTCCACTGC GCAATCCGGC CATTCTGGTG GGACAGAACG ACTTGACCAC CCTGTCCTAC 
CTGCATGAGC CGGGGGTGTT GCACAATCTG CGTGTCCGCT TCTGCGAGCG CCAGATTATC 
TACACCTACT GCGGCATCAT TCTGGTGGCC ATCAACCCGT ACGCGGAGAT GCCTCTTTAC 
GGGCCCAGCA TAATCCGAGC GTATCGGGGT CATGCTATGG GTGATCTGGA GCCGCACATC 
TTTGCCCTGG CGGAGGAGGC GTACACGAAA CTGGAGCGCG AGAACTGCAA CCTGAGCATC 
ATCGTCAGTG GGGAGTCGGG TGCGGGCAAG ACGGTGTCCG CCAAATACGC CATGAGGTAC 
TTTGCCGCTG TTGGAGGTTC CGAGTCGGAG ACCCAGGTCG AACGCAAGGT GCTGGCATCT 
TCGCCGATCA TGGAAGCCTT CGGAAATGCC AAGACGACCC GGAATGACAA CAGTTCCCGC 
TTTGGAAAGT TTACCAAGCT GCTGTTCCGG AACCAGATGG GTGTGATGTT CCTGCAGGGA 
GCCACTATGC ACACCTACCT ACTGGAGAAG TCACGTGTGG TGTACCAGGC CCAGGGAGAG 
CGCAACTATC ACATATTCTA TCAGCTGTGC GCGGCGCGAT CGAAGTACCC TGAACTGGTG 
CTGGATCACC AGGACAAATT CCAGTTTCTG AACATGGGTG GCGCTCCTGA AATTGAACGA 
GTTTCGGATG CGGAGCAGTT TAACGAAACC GTGCAGGCCA TGACAGTTCT GGGCTTCTCC 
ATTCAACAGA TCGCTGATAT CGTAAAGATC CTGGCAGGAA TACTCCATTT AGGAAACATT 
CAGGTTTCCA AGAAGTTCAA CGAGGGCAGC GAAGAGGAGG ACAGTGACTC TTGCGATATA 
TTTCATAACG ACATCCACCT GCAGATCACC GCCGATCTAC TGCGGGTGAG CGCCGATGAT 
CTGCGCCGGT GGCTTTTGAT GCGTAAGATA GAGTCGGTCA ATGAATATGT GCTGATACCG 
AATAGCATTG AGGCGGCTCA GGCGGCTCGA GACGCTCTGG CCAAGCACAT CTATGCGAAA 
CTGTTTCAGT ATATAGTCGG TGTGCTGAAC AAGAGCCTCA ACAACGGTAG CAAGCAGTGC 
AGCTTCATTG GCGTCCTCGA TATCTACGGC TTCGAAACGT TCGAGGTGAA CTCCTTTGAA 
CAATTTTGCA TAAACTATGC GAACGAAAAG CTTCAGCAGC AGTTCAACCA GCATGTCTTC 
AAGCTGGAGC AGGAGGAGTA TCTTAAGGAA GGAATCACCT GGACGATGAT TGACTTTTAC 
GACAATCAAC CGTGTATCGA TCTAATTGAA TCTCGCTTGG GAGTGCTGGA CCTGCTCGAC 
GAGGAGTGTC GAATGCCAAA GGGCTCGGAC GAGAGCTGGG CTGGCAAGCT CATCGGAAAG 
TGCAATAAAT TTCCGCATTT CGAGAAGCCA CGCTTTGGCA CAACCAGCTT CTTTATCAAA 
CATTTCTCGG ACACGGTCGA GTATGACGTG AACGGATTCT TGGAAAAGAA TCGTGACACA 
GTCTCCAAGG AGTTGACGCA AGTGCTAAGC GAGTCCAACA TGTCTCTGGC CAAGCAGGTG 
ATGACCCTGG AGGAAATAGA TACTCTGTGC GTGGATTCCG CTAAATCCTC CACCTTGGGC 
GGCCGCGTCG TGATCAGTGC TGGCCGCAAA CAGGTGGTGC CATCCAAGCA GCATAGAAAA 
ACGGTGGGAT CGCAGTTCCA GGAGAGTCTG GCGTCGCTGA TATCTACGTT ACATGCCACA 
ACTCCGCACT ATGTGCGCTG CATCAAGCCC AACGATGACA AAGTCGCCTT TAAGTGGGAG 
ACGGCCAAGA TCATACAGCA GTTAAGGGCC TGTGGTGTGC TGGAAACGGT GCGCATCTCC 
GCAGCGGGAT TCCCCTCGAG ATGGCTCTAT CCCGACTTCT ATATGCGGTA CCAGCTGCTG 
GTTTACCGCT CCAAACTCGA CAAAAACGAC ATGAAGCTGT CGTGCCGGAA CATTGTGATG 
AAGTGGATCC AAGACGAAGA TAAGTACCGA TTTGGCAACA CGCAGATTTT CTTCCGCGCC 
GGCCAAGTGG CCTTCCTTGA ACAGGTTCGG GCTAATCTGC GCAAGAAGTA TATCACCATT 
GTGCAGTCGG TTGTGCGGCG ATTCGTCTAC CGGCGCCAGT TCCTGCGCAT TCAGAAAGTA 
ATTAATGGCA TTCAGAAACA TGCGCGCGGA TATCTTGCTC GCGAGCGTAC TCAGAAAATG 
CGCGAAGCTC GTGCGGGATT AATCCTGTCG AAGTACGCCC GCGGTTGGTT ATGCCGTCGT 
CGTTACTTGC GCCTACGCCA CTCCATTTCC GGCATACAGA CCTACGCCCG CGGCATGCTG 
GCGCGCAACA AGTTCCACGC GATGCGGGAT CACTACCGGG CAGTTCAGAT CCAGCGTTTC 
GTGCGTGGTG CTTTGGCACG GCGAGCTTAC CAAAAGCGGC GTCGCAACAT CATCATTTGT 
CAAGCGGCGA TTCGGAGATT CTTGGCCCGT CGTAAGTTTA AACGCATGAA GGCCGAGGCC 
AAGACCATCT CGCACATGGA AAACAAATAC ATGGGGCTGG AAAACAAGAT TATTTCCATG 
CAGCAGCGGA TCGATGAGCT GAATCGCGAC AACAGTAATC TGAAGCACAA GACCAGCGAA 
ATCAGTGTAT TGAAAATGAA GCTTGAGCTG AAGAAGACCC TGGAGGCTGA ATTCAAAAAT 
GTCAAGGCCG CCTGCCAGGA CAAGGACAAG CTGATCGAAG CACTTAACAA GCAGTTGGAG 
GCGGAGCGAG ACGAAAAAAT GCAGTTGCTG GAGGAGAACG GACATGCTCA AGAGGAGTGG 
ATCAGCCAGA AGCAGACGTG GCGCCAAGAG AACGAGGAGC TGCGCCGTCA GATAGACGAG 
ATAATCGATA TGGCAAAGAA CGCAGAAGTC AACCAGCGTA ACCAGGAGGA CCGAATGCTA 
GCCGAGATTG ATAACAGGGA GCTCAACGAG GCCTACCAAC GAGCTATTAA GGACAAGGAG 
GTCATCGAGA ACGAAAACTT CATGCTGAAG GAAGAGCTCA GTCGATTAAC GGCTGGCAGT 
TTCAGTTTGC ACGCCCGCAA GGCTAGCAAC GCCTCCAGCC AAAACGAGGA CGATGTGGGA 
TACGCCTCCG CCAAGAACAC TCTGGATATC AATCGGCCCC CGGATTTGTT AAGCAAAAAT 
TACTCGTACA ATGACTCTAC CAGTCTGGTG GTGAAGTTGA GATCCATTCT CGAGGAGGAG 
AAGCAAAAGC ACAAGGTCTT GCAGGAGCAG TACATTAAGT TGTCCAGTCG GCATAAGCCC 
ACCGAGGATT CCTTCCGCGT CTCCGAGCTT GAGGTAGAGA ATGAAAAGCT GCGCAGCGAG 
TACGATCAGC TGCGAACGAG CATTAAACAC GGTGTTGAGA TCAACGAGCT CAATGCACAG 
CATGCCGCCT TGCAGGAAGA GGTACGTAGG CGGCGCGAGG AGTGCATCCA ATTAAAGGCA 
GTCCTGCTGC AGCAGAGCCA GTCCATGAGA TCGCTCGAGC CGGAAAGTCT ACAGATGCGT 
GGCAACGATG TCAACGAACT GATGGAAGCC TTCCATTCCC AGAAGCTAAT TAATCGTCAA 
TTGGAGTCTG AGCTCAAGGC CATCACCGAG GAGCACAACA GTAAGCTCGT GGAGATGACA 
CAGGAGATCG AGAGATTGAA CAATGAGAAG GATGAGCTGC AAAAGGTAAT GTTCGAGAGC 
ATCGACGAGT TCGAAGATTC CAATGTGGAT ACGCTGAGAC AGAACGATCG CTATCTGCGA 
CGAGAACTGC AGAAGGCTGT AGCCCAGTTC CTGCTCGTTC AGGAGGAGCT CAAACTGGCA 
AATGCCAAGC TTAAAGCTTA TCGGCAGGAT GGAGGCCAGC TGGAGCACAA GATAGAGGAG 
GAGATGATTC GCAACAAGTC CAACGGAACG TCCGCCGATG TAGGCGCGAA TGTGACGAAG 
CAAAAGTCTC AGAATCCGCA AGGGCTGATG AAGTTCCACA GCAGCGATCT GGACAAGATC 
TTGCAACGCC TGCTTAGCGC CTTGACTCCA CGCACAGTGG TCGGGCTCCT TCCTGGTTTT 
CCAGCATATC TCATCTTTAT GTGTATTCGA TACACCGATC TGACAAATGC CGACGATGAT 
GTGCGCGAGT TGCTAAGCAA GTTCGTTATT CAGATTAAGA AAATGCATCG TACGCCGCAT 
CCGATCGAGA ATCGTGTTAT TTGGCTCGTC AATTCCATTA CGCTGCTAAA TCTTATGAAG 
CAATACGGCG ACGTGGATGA GTACGTCAAG TTCAATACTG AGAAGCAGAA TCAGCAGCAG 
CTGAAGAACT TCAATCTCTT TGAATACCGT CGCGTAATTC TTGATTTAAT TGTGAACCTG 
TACCAGGCGC TGATCATGCA GATCCAGGGT CTGTTGGACC CAAAAATAGT GCCAGCGATT 
CTCAACAATG ATGAGATTCA GCGTGGGCGG CAGGCGCACG GAATGCGTAG TCGGGCCACG 
TCGATTGGAG CATCCTCGTC ACCGGAGCAC GGTGGCGGTC CGGCCTGGAA GCAACTGATC 
GGGCAGCTGG AGCATTTCTA CAAACAGTTT CAGCACTTCG GCTTGGACAA CTGCTATGCG 
GAGCAGATAT TCCATCAACT GCTTTACTTC ATTTGCGCTG TGGCCCTTAA TTGTCTGATG 
CTTAGGGGCG ATATTTGCAT GTGGGAGACT GGCATGATAA TCCGCTACAA TATCGGCTGC 
ATTGAGGATT GGGTGCGCAG TAAAAAGATG TCTAACGATG TGCTGACAGC TTTGGCGCCT 
CTGAATCAGG TCTCCCAATT GCTGCAGTCT CGGAAGAGCG AGCAGGATGT TCAGACCATT 
TGTGATCTGT GTACTTCTCT GAGCACGGCG CAGGTCCTCA AGGTGATGAA GTCCTACAAA 
CTGGACGATT ATGAGAGCGA AATAACGAAC GTTTTTCTGG AGAAACTAAC CGAGAAACTG 
AACGCCCGAC AAATGCAAAA GAGCAATAGT GACGAATTCA CCATAGACCA GAAGTTCATT 
CAGCCATTTA AGGTTGTCTT CAGGTATAGT GACATCAAGC TGGAGGATAT TGAACTACCG 
TCGCATCTTA ATCTGGACGA GTTCCTTACA AAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477186.1
CDS	333..5711
Translation	MSSEEMLYAQGAKIWVPHADLVWESATLEESYRKGAGFLKICTDSGKLKEVKLKADGSDLPPLRNPAILVGQNDLTTLSYLHEPGVLHNLRVRFCERQIIYTYCGIILVAINPYAEMPLYGPSIIRAYRGHAMGDLEPHIFALAEEAYTKLERENCNLSIIVSGESGAGKTVSAKYAMRYFAAVGGSESETQVERKVLASSPIMEAFGNAKTTRNDNSSRFGKFTKLLFRNQMGVMFLQGATMHTYLLEKSRVVYQAQGERNYHIFYQLCAARSKYPELVLDHQDKFQFLNMGGAPEIERVSDAEQFNETVQAMTVLGFSIQQIADIVKILAGILHLGNIQVSKKFNEGSEEEDSDSCDIFHNDIHLQITADLLRVSADDLRRWLLMRKIESVNEYVLIPNSIEAAQAARDALAKHIYAKLFQYIVGVLNKSLNNGSKQCSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNQHVFKLEQEEYLKEGITWTMIDFYDNQPCIDLIESRLGVLDLLDEECRMPKGSDESWAGKLIGKCNKFPHFEKPRFGTTSFFIKHFSDTVEYDVNGFLEKNRDTVSKELTQVLSESNMSLAKQVMTLEEIDTLCVDSAKSSTLGGRVVISAGRKQVVPSKQHRKTVGSQFQESLASLISTLHATTPHYVRCIKPNDDKVAFKWETAKIIQQLRACGVLETVRISAAGFPSRWLYPDFYMRYQLLVYRSKLDKNDMKLSCRNIVMKWIQDEDKYRFGNTQIFFRAGQVAFLEQVRANLRKKYITIVQSVVRRFVYRRQFLRIQKVINGIQKHARGYLARERTQKMREARAGLILSKYARGWLCRRRYLRLRHSISGIQTYARGMLARNKFHAMRDHYRAVQIQRFVRGALARRAYQKRRRNIIICQAAIRRFLARRKFKRMKAEAKTISHMENKYMGLENKIISMQQRIDELNRDNSNLKHKTSEISVLKMKLELKKTLEAEFKNVKAACQDKDKLIEALNKQLEAERDEKMQLLEENGHAQEEWISQKQTWRQENEELRRQIDEIIDMAKNAEVNQRNQEDRMLAEIDNRELNEAYQRAIKDKEVIENENFMLKEELSRLTAGSFSLHARKASNASSQNEDDVGYASAKNTLDINRPPDLLSKNYSYNDSTSLVVKLRSILEEEKQKHKVLQEQYIKLSSRHKPTEDSFRVSELEVENEKLRSEYDQLRTSIKHGVEINELNAQHAALQEEVRRRREECIQLKAVLLQQSQSMRSLEPESLQMRGNDVNELMEAFHSQKLINRQLESELKAITEEHNSKLVEMTQEIERLNNEKDELQKVMFESIDEFEDSNVDTLRQNDRYLRRELQKAVAQFLLVQEELKLANAKLKAYRQDGGQLEHKIEEEMIRNKSNGTSADVGANVTKQKSQNPQGLMKFHSSDLDKILQRLLSALTPRTVVGLLPGFPAYLIFMCIRYTDLTNADDDVRELLSKFVIQIKKMHRTPHPIENRVIWLVNSITLLNLMKQYGDVDEYVKFNTEKQNQQQLKNFNLFEYRRVILDLIVNLYQALIMQIQGLLDPKIVPAILNNDEIQRGRQAHGMRSRATSIGASSSPEHGGGPAWKQLIGQLEHFYKQFQHFGLDNCYAEQIFHQLLYFICAVALNCLMLRGDICMWETGMIIRYNIGCIEDWVRSKKMSNDVLTALAPLNQVSQLLQSRKSEQDVQTICDLCTSLSTAQVLKVMKSYKLDDYESEITNVFLEKLTEKLNARQMQKSNSDEFTIDQKFIQPFKVVFRYSDIKLEDIELPSHLNLDEFLTKI

Target ORF information:

RefSeq Version	NM_057838.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dilute class unconventional myosin (didum), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057838.4

ORF Insert Sequence:

ATGTCTAGCG AGGAGATGCT ATACGCGCAG GGCGCCAAGA TCTGGGTGCC CCATGCGGAT 
CTGGTGTGGG AGAGCGCCAC CTTGGAGGAG AGCTACCGCA AGGGCGCCGG CTTCTTGAAG 
ATATGTACGG ACTCCGGAAA ACTGAAAGAG GTCAAGCTAA AGGCCGATGG CAGCGATCTG 
CCTCCACTGC GCAATCCGGC CATTCTGGTG GGACAGAACG ACTTGACCAC CCTGTCCTAC 
CTGCATGAGC CGGGGGTGTT GCACAATCTG CGTGTCCGCT TCTGCGAGCG CCAGATTATC 
TACACCTACT GCGGCATCAT TCTGGTGGCC ATCAACCCGT ACGCGGAGAT GCCTCTTTAC 
GGGCCCAGCA TAATCCGAGC GTATCGGGGT CATGCTATGG GTGATCTGGA GCCGCACATC 
TTTGCCCTGG CGGAGGAGGC GTACACGAAA CTGGAGCGCG AGAACTGCAA CCTGAGCATC 
ATCGTCAGTG GGGAGTCGGG TGCGGGCAAG ACGGTGTCCG CCAAATACGC CATGAGGTAC 
TTTGCCGCTG TTGGAGGTTC CGAGTCGGAG ACCCAGGTCG AACGCAAGGT GCTGGCATCT 
TCGCCGATCA TGGAAGCCTT CGGAAATGCC AAGACGACCC GGAATGACAA CAGTTCCCGC 
TTTGGAAAGT TTACCAAGCT GCTGTTCCGG AACCAGATGG GTGTGATGTT CCTGCAGGGA 
GCCACTATGC ACACCTACCT ACTGGAGAAG TCACGTGTGG TGTACCAGGC CCAGGGAGAG 
CGCAACTATC ACATATTCTA TCAGCTGTGC GCGGCGCGAT CGAAGTACCC TGAACTGGTG 
CTGGATCACC AGGACAAATT CCAGTTTCTG AACATGGGTG GCGCTCCTGA AATTGAACGA 
GTTTCGGATG CGGAGCAGTT TAACGAAACC GTGCAGGCCA TGACAGTTCT GGGCTTCTCC 
ATTCAACAGA TCGCTGATAT CGTAAAGATC CTGGCAGGAA TACTCCATTT AGGAAACATT 
CAGGTTTCCA AGAAGTTCAA CGAGGGCAGC GAAGAGGAGG ACAGTGACTC TTGCGATATA 
TTTCATAACG ACATCCACCT GCAGATCACC GCCGATCTAC TGCGGGTGAG CGCCGATGAT 
CTGCGCCGGT GGCTTTTGAT GCGTAAGATA GAGTCGGTCA ATGAATATGT GCTGATACCG 
AATAGCATTG AGGCGGCTCA GGCGGCTCGA GACGCTCTGG CCAAGCACAT CTATGCGAAA 
CTGTTTCAGT ATATAGTCGG TGTGCTGAAC AAGAGCCTCA ACAACGGTAG CAAGCAGTGC 
AGCTTCATTG GCGTCCTCGA TATCTACGGC TTCGAAACGT TCGAGGTGAA CTCCTTTGAA 
CAATTTTGCA TAAACTATGC GAACGAAAAG CTTCAGCAGC AGTTCAACCA GCATGTCTTC 
AAGCTGGAGC AGGAGGAGTA TCTTAAGGAA GGAATCACCT GGACGATGAT TGACTTTTAC 
GACAATCAAC CGTGTATCGA TCTAATTGAA TCTCGCTTGG GAGTGCTGGA CCTGCTCGAC 
GAGGAGTGTC GAATGCCAAA GGGCTCGGAC GAGAGCTGGG CTGGCAAGCT CATCGGAAAG 
TGCAATAAAT TTCCGCATTT CGAGAAGCCA CGCTTTGGCA CAACCAGCTT CTTTATCAAA 
CATTTCTCGG ACACGGTCGA GTATGACGTG AACGGATTCT TGGAAAAGAA TCGTGACACA 
GTCTCCAAGG AGTTGACGCA AGTGCTAAGC GAGTCCAACA TGTCTCTGGC CAAGCAGGTG 
ATGACCCTGG AGGAAATAGA TACTCTGTGC GTGGATTCCG CTAAATCCTC CACCTTGGGC 
GGCCGCGTCG TGATCAGTGC TGGCCGCAAA CAGGTGGTGC CATCCAAGCA GCATAGAAAA 
ACGGTGGGAT CGCAGTTCCA GGAGAGTCTG GCGTCGCTGA TATCTACGTT ACATGCCACA 
ACTCCGCACT ATGTGCGCTG CATCAAGCCC AACGATGACA AAGTCGCCTT TAAGTGGGAG 
ACGGCCAAGA TCATACAGCA GTTAAGGGCC TGTGGTGTGC TGGAAACGGT GCGCATCTCC 
GCAGCGGGAT TCCCCTCGAG ATGGCTCTAT CCCGACTTCT ATATGCGGTA CCAGCTGCTG 
GTTTACCGCT CCAAACTCGA CAAAAACGAC ATGAAGCTGT CGTGCCGGAA CATTGTGATG 
AAGTGGATCC AAGACGAAGA TAAGTACCGA TTTGGCAACA CGCAGATTTT CTTCCGCGCC 
GGCCAAGTGG CCTTCCTTGA ACAGGTTCGG GCTAATCTGC GCAAGAAGTA TATCACCATT 
GTGCAGTCGG TTGTGCGGCG ATTCGTCTAC CGGCGCCAGT TCCTGCGCAT TCAGAAAGTA 
ATTAATGGCA TTCAGAAACA TGCGCGCGGA TATCTTGCTC GCGAGCGTAC TCAGAAAATG 
CGCGAAGCTC GTGCGGGATT AATCCTGTCG AAGTACGCCC GCGGTTGGTT ATGCCGTCGT 
CGTTACTTGC GCCTACGCCA CTCCATTTCC GGCATACAGA CCTACGCCCG CGGCATGCTG 
GCGCGCAACA AGTTCCACGC GATGCGGGAT CACTACCGGG CAGTTCAGAT CCAGCGTTTC 
GTGCGTGGTG CTTTGGCACG GCGAGCTTAC CAAAAGCGGC GTCGCAACAT CATCATTTGT 
CAAGCGGCGA TTCGGAGATT CTTGGCCCGT CGTAAGTTTA AACGCATGAA GGCCGAGGCC 
AAGACCATCT CGCACATGGA AAACAAATAC ATGGGGCTGG AAAACAAGAT TATTTCCATG 
CAGCAGCGGA TCGATGAGCT GAATCGCGAC AACAGTAATC TGAAGCACAA GACCAGCGAA 
ATCAGTGTAT TGAAAATGAA GCTTGAGCTG AAGAAGACCC TGGAGGCTGA ATTCAAAAAT 
GTCAAGGCCG CCTGCCAGGA CAAGGACAAG CTGATCGAAG CACTTAACAA GCAGTTGGAG 
GCGGAGCGAG ACGAAAAAAT GCAGTTGCTG GAGGAGAACG GACATGCTCA AGAGGAGTGG 
ATCAGCCAGA AGCAGACGTG GCGCCAAGAG AACGAGGAGC TGCGCCGTCA GATAGACGAG 
ATAATCGATA TGGCAAAGAA CGCAGAAGTC AACCAGCGTA ACCAGGAGGA CCGAATGCTA 
GCCGAGATTG ATAACAGGGA GCTCAACGAG GCCTACCAAC GAGCTATTAA GGACAAGGAG 
GTCATCGAGA ACGAAAACTT CATGCTGAAG GAAGAGCTCA GTCGATTAAC GGCTGGCAGT 
TTCAGTTTGC ACGCCCGCAA GGCTAGCAAC GCCTCCAGCC AAAACGAGGA CGATGTGGGA 
TACGCCTCCG CCAAGAACAC TCTGGATATC AATCGGCCCC CGGATTTGTT AAGCAAAAAT 
TACTCGTACA ATGACTCTAC CAGTCTGGTG GTGAAGTTGA GATCCATTCT CGAGGAGGAG 
AAGCAAAAGC ACAAGGTCTT GCAGGAGCAG TACATTAAGT TGTCCAGTCG GCATAAGCCC 
ACCGAGGATT CCTTCCGCGT CTCCGAGCTT GAGGTAGAGA ATGAAAAGCT GCGCAGCGAG 
TACGATCAGC TGCGAACGAG CATTAAACAC GGTGTTGAGA TCAACGAGCT CAATGCACAG 
CATGCCGCCT TGCAGGAAGA GGTACGTAGG CGGCGCGAGG AGTGCATCCA ATTAAAGGCA 
GTCCTGCTGC AGCAGAGCCA GTCCATGAGA TCGCTCGAGC CGGAAAGTCT ACAGATGCGT 
GGCAACGATG TCAACGAACT GATGGAAGCC TTCCATTCCC AGAAGCTAAT TAATCGTCAA 
TTGGAGTCTG AGCTCAAGGC CATCACCGAG GAGCACAACA GTAAGCTCGT GGAGATGACA 
CAGGAGATCG AGAGATTGAA CAATGAGAAG GATGAGCTGC AAAAGGTAAT GTTCGAGAGC 
ATCGACGAGT TCGAAGATTC CAATGTGGAT ACGCTGAGAC AGAACGATCG CTATCTGCGA 
CGAGAACTGC AGAAGGCTGT AGCCCAGTTC CTGCTCGTTC AGGAGGAGCT CAAACTGGCA 
AATGCCAAGC TTAAAGCTTA TCGGCAGGAT GGAGGCCAGC TGGAGCACAA GATAGAGGAG 
GAGATGATTC GCAACAAGTC CAACGGAACG TCCGCCGATG TAGGCGCGAA TGTGACGAAG 
CAAAAGTCTC AGAATCCGCA AGGGCTGATG AAGTTCCACA GCAGCGATCT GGACAAGATC 
TTGCAACGCC TGCTTAGCGC CTTGACTCCA CGCACAGTGG TCGGGCTCCT TCCTGGTTTT 
CCAGCATATC TCATCTTTAT GTGTATTCGA TACACCGATC TGACAAATGC CGACGATGAT 
GTGCGCGAGT TGCTAAGCAA GTTCGTTATT CAGATTAAGA AAATGCATCG TACGCCGCAT 
CCGATCGAGA ATCGTGTTAT TTGGCTCGTC AATTCCATTA CGCTGCTAAA TCTTATGAAG 
CAATACGGCG ACGTGGATGA GTACGTCAAG TTCAATACTG AGAAGCAGAA TCAGCAGCAG 
CTGAAGAACT TCAATCTCTT TGAATACCGT CGCGTAATTC TTGATTTAAT TGTGAACCTG 
TACCAGGCGC TGATCATGCA GATCCAGGGT CTGTTGGACC CAAAAATAGT GCCAGCGATT 
CTCAACAATG ATGAGATTCA GCGTGGGCGG CAGGCGCACG GAATGCGTAG TCGGGCCACG 
TCGATTGGAG CATCCTCGTC ACCGGAGCAC GGTGGCGGTC CGGCCTGGAA GCAACTGATC 
GGGCAGCTGG AGCATTTCTA CAAACAGTTT CAGCACTTCG GCTTGGACAA CTGCTATGCG 
GAGCAGATAT TCCATCAACT GCTTTACTTC ATTTGCGCTG TGGCCCTTAA TTGTCTGATG 
CTTAGGGGCG ATATTTGCAT GTGGGAGACT GGCATGATAA TCCGCTACAA TATCGGCTGC 
ATTGAGGATT GGGTGCGCAG TAAAAAGATG TCTAACGATG TGCTGACAGC TTTGGCGCCT 
CTGAATCAGG TCTCCCAATT GCTGCAGTCT CGGAAGAGCG AGCAGGATGT TCAGACCATT 
TGTGATCTGT GTACTTCTCT GAGCACGGCG CAGGTCCTCA AGGTGATGAA GTCCTACAAA 
CTGGACGATT ATGAGAGCGA AATAACGAAC GTTTTTCTGG AGAAACTAAC CGAGAAACTG 
AACGCCCGAC AAATGCAAAA GAGCAATAGT GACGAATTCA CCATAGACCA GAAGTTCATT 
CAGCCATTTA AGGTTGTCTT CAGGTATAGT GACATCAAGC TGGAGGATAT TGAACTACCG 
TCGCATCTTA ATCTGGACGA GTTCCTTACA AAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

didum ( NM_165535.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724569.1 Drosophila melanogaster dilute class unconventional myosin (didum), transcript variant C, mRNA.

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CloneID	OFa12466
Clone ID Related Accession (Same CDS sequence)	NM_165535.2
Accession Version	NM_165535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dilute class unconventional myosin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165535.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTAGCG AGGAGATGCT ATACGCGCAG GGCGCCAAGA TCTGGGTGCC CCATGCGGAT 
CTGGTGTGGG AGAGCGCCAC CTTGGAGGAG AGCTACCGCA AGGGCGCCGG CTTCTTGAAG 
ATATGTACGG ACTCCGGAAA ACTGAAAGAG GTCAAGCTAA AGGCCGATGG CAGCGATCTG 
CCTCCACTGC GCAATCCGGC CATTCTGGTG GGACAGAACG ACTTGACCAC CCTGTCCTAC 
CTGCATGAGC CGGGGGTGTT GCACAATCTG CGTGTCCGCT TCTGCGAGCG CCAGATTATC 
TACACCTACT GCGGCATCAT TCTGGTGGCC ATCAACCCGT ACGCGGAGAT GCCTCTTTAC 
GGGCCCAGCA TAATCCGAGC GTATCGGGGT CATGCTATGG GTGATCTGGA GCCGCACATC 
TTTGCCCTGG CGGAGGAGGC GTACACGAAA CTGGAGCGCG AGAACTGCAA CCTGAGCATC 
ATCGTCAGTG GGGAGTCGGG TGCGGGCAAG ACGGTGTCCG CCAAATACGC CATGAGGTAC 
TTTGCCGCTG TTGGAGGTTC CGAGTCGGAG ACCCAGGTCG AACGCAAGGT GCTGGCATCT 
TCGCCGATCA TGGAAGCCTT CGGAAATGCC AAGACGACCC GGAATGACAA CAGTTCCCGC 
TTTGGAAAGT TTACCAAGCT GCTGTTCCGG AACCAGATGG GTGTGATGTT CCTGCAGGGA 
GCCACTATGC ACACCTACCT ACTGGAGAAG TCACGTGTGG TGTACCAGGC CCAGGGAGAG 
CGCAACTATC ACATATTCTA TCAGCTGTGC GCGGCGCGAT CGAAGTACCC TGAACTGGTG 
CTGGATCACC AGGACAAATT CCAGTTTCTG AACATGGGTG GCGCTCCTGA AATTGAACGA 
GTTTCGGATG CGGAGCAGTT TAACGAAACC GTGCAGGCCA TGACAGTTCT GGGCTTCTCC 
ATTCAACAGA TCGCTGATAT CGTAAAGATC CTGGCAGGAA TACTCCATTT AGGAAACATT 
CAGGTTTCCA AGAAGTTCAA CGAGGGCAGC GAAGAGGAGG ACAGTGACTC TTGCGATATA 
TTTCATAACG ACATCCACCT GCAGATCACC GCCGATCTAC TGCGGGTGAG CGCCGATGAT 
CTGCGCCGGT GGCTTTTGAT GCGTAAGATA GAGTCGGTCA ATGAATATGT GCTGATACCG 
AATAGCATTG AGGCGGCTCA GGCGGCTCGA GACGCTCTGG CCAAGCACAT CTATGCGAAA 
CTGTTTCAGT ATATAGTCGG TGTGCTGAAC AAGAGCCTCA ACAACGGTAG CAAGCAGTGC 
AGCTTCATTG GCGTCCTCGA TATCTACGGC TTCGAAACGT TCGAGGTGAA CTCCTTTGAA 
CAATTTTGCA TAAACTATGC GAACGAAAAG CTTCAGCAGC AGTTCAACCA GCATGTCTTC 
AAGCTGGAGC AGGAGGAGTA TCTTAAGGAA GGAATCACCT GGACGATGAT TGACTTTTAC 
GACAATCAAC CGTGTATCGA TCTAATTGAA TCTCGCTTGG GAGTGCTGGA CCTGCTCGAC 
GAGGAGTGTC GAATGCCAAA GGGCTCGGAC GAGAGCTGGG CTGGCAAGCT CATCGGAAAG 
TGCAATAAAT TTCCGCATTT CGAGAAGCCA CGCTTTGGCA CAACCAGCTT CTTTATCAAA 
CATTTCTCGG ACACGGTCGA GTATGACGTG AACGGATTCT TGGAAAAGAA TCGTGACACA 
GTCTCCAAGG AGTTGACGCA AGTGCTAAGC GAGTCCAACA TGTCTCTGGC CAAGCAGGTG 
ATGACCCTGG AGGAAATAGA TACTCTGTGC GTGGATTCCG CTAAATCCTC CACCTTGGGC 
GGCCGCGTCG TGATCAGTGC TGGCCGCAAA CAGCAAGGGA ATGACACACG TCGAAGAGTG 
GTGCCATCCA AGCAGCATAG AAAAACGGTG GGATCGCAGT TCCAGGAGAG TCTGGCGTCG 
CTGATATCTA CGTTACATGC CACAACTCCG CACTATGTGC GCTGCATCAA GCCCAACGAT 
GACAAAGTCG CCTTTAAGTG GGAGACGGCC AAGATCATAC AGCAGTTAAG GGCCTGTGGT 
GTGCTGGAAA CGGTGCGCAT CTCCGCAGCG GGATTCCCCT CGAGATGGCT CTATCCCGAC 
TTCTATATGC GGTACCAGCT GCTGGTTTAC CGCTCCAAAC TCGACAAAAA CGACATGAAG 
CTGTCGTGCC GGAACATTGT GATGAAGTGG ATCCAAGACG AAGATAAGTA CCGATTTGGC 
AACACGCAGA TTTTCTTCCG CGCCGGCCAA GTGGCCTTCC TTGAACAGGT TCGGGCTAAT 
CTGCGCAAGA AGTATATCAC CATTGTGCAG TCGGTTGTGC GGCGATTCGT CTACCGGCGC 
CAGTTCCTGC GCATTCAGAA AGTAATTAAT GGCATTCAGA AACATGCGCG CGGATATCTT 
GCTCGCGAGC GTACTCAGAA AATGCGCGAA GCTCGTGCGG GATTAATCCT GTCGAAGTAC 
GCCCGCGGTT GGTTATGCCG TCGTCGTTAC TTGCGCCTAC GCCACTCCAT TTCCGGCATA 
CAGACCTACG CCCGCGGCAT GCTGGCGCGC AACAAGTTCC ACGCGATGCG GGATCACTAC 
CGGGCAGTTC AGATCCAGCG TTTCGTGCGT GGTGCTTTGG CACGGCGAGC TTACCAAAAG 
CGGCGTCGCA ACATCATCAT TTGTCAAGCG GCGATTCGGA GATTCTTGGC CCGTCGTAAG 
TTTAAACGCA TGAAGGCCGA GGCCAAGACC ATCTCGCACA TGGAAAACAA ATACATGGGG 
CTGGAAAACA AGATTATTTC CATGCAGCAG CGGATCGATG AGCTGAATCG CGACAACAGT 
AATCTGAAGC ACAAGACCAG CGAAATCAGT GTATTGAAAA TGAAGCTTGA GCTGAAGAAG 
ACCCTGGAGG CTGAATTCAA AAATGTCAAG GCCGCCTGCC AGGACAAGGA CAAGCTGATC 
GAAGCACTTA ACAAGCAGTT GGAGGCGGAG CGAGACGAAA AAATGCAGTT GCTGGAGGAG 
AACGGACATG CTCAAGAGGA GTGGATCAGC CAGAAGCAGA CGTGGCGCCA AGAGAACGAG 
GAGCTGCGCC GTCAGATAGA CGAGATAATC GATATGGCAA AGAACGCAGA AGTCAACCAG 
CGTAACCAGG AGGACCGAAT GCTAGCCGAG ATTGATAACA GGGAGCTCAA CGAGGCCTAC 
CAACGAGCTA TTAAGGACAA GGAGGTCATC GAGAACGAAA ACTTCATGCT GAAGGAAGAG 
CTCAGTCGAT TAACGGCTGG CAGTTTCAGT TTGCACGCCC GCAAGGCTAG CAACGCCTCC 
AGCCAAAACG AGGACGATGT GGGATACGCC TCCGCCAAGA ACACTCTGGA TATCAATCGG 
CCCCCGGATT TGTTAAGCAA AAATTACTCG TACAATGACT CTACCAGTCT GGTGGTGAAG 
TTGAGATCCA TTCTCGAGGA GGAGAAGCAA AAGCACAAGG TCTTGCAGGA GCAGTACATT 
AAGTTGTCCA GTCGGCATAA GCCCACCGAG GATTCCTTCC GCGTCTCCGA GCTTGAGGTA 
GAGAATGAAA AGCTGCGCAG CGAGTACGAT CAGCTGCGAA CGAGCATTAA ACACGGTGTT 
GAGATCAACG AGCTCAATGC ACAGCATGCC GCCTTGCAGG AAGAGGTACG TAGGCGGCGC 
GAGGAGTGCA TCCAATTAAA GGCAGTCCTG CTGCAGCAGA GCCAGTCCAT GAGATCGCTC 
GAGCCGGAAA GTCTACAGAT GCGTGGCAAC GATGTCAACG AACTGATGGA AGCCTTCCAT 
TCCCAGAAGC TAATTAATCG TCAATTGGAG TCTGAGCTCA AGGCCATCAC CGAGGAGCAC 
AACAGTAAGC TCGTGGAGAT GACACAGGAG ATCGAGAGAT TGAACAATGA GAAGGATGAG 
CTGCAAAAGG TAATGTTCGA GAGCATCGAC GAGTTCGAAG ATTCCAATGT GGATACGCTG 
AGACAGAACG ATCGCTATCT GCGACGAGAA CTGCAGAAGG CTGTAGCCCA GTTCCTGCTC 
GTTCAGGAGG AGCTCAAACT GGCAAATGCC AAGCTTAAAG CTTATCGGCA GGATGGAGGC 
CAGCTGGAGC ACAAGATAGA GGAGGAGATG ATTCGCAACA AGTCCAACGG AACGTCCGCC 
GATGTAGGCG CGAATGTGAC GAAGCAAAAG TCTCAGAATC CGCAAGGGCT GATGAAGTTC 
CACAGCAGCG ATCTGGACAA GATCTTGCAA CGCCTGCTTA GCGCCTTGAC TCCACGCACA 
GTGGTCGGGC TCCTTCCTGG TTTTCCAGCA TATCTCATCT TTATGTGTAT TCGATACACC 
GATCTGACAA ATGCCGACGA TGATGTGCGC GAGTTGCTAA GCAAGTTCGT TATTCAGATT 
AAGAAAATGC ATCGTACGCC GCATCCGATC GAGAATCGTG TTATTTGGCT CGTCAATTCC 
ATTACGCTGC TAAATCTTAT GAAGCAATAC GGCGACGTGG ATGAGTACGT CAAGTTCAAT 
ACTGAGAAGC AGAATCAGCA GCAGCTGAAG AACTTCAATC TCTTTGAATA CCGTCGCGTA 
ATTCTTGATT TAATTGTGAA CCTGTACCAG GCGCTGATCA TGCAGATCCA GGGTCTGTTG 
GACCCAAAAA TAGTGCCAGC GATTCTCAAC AATGATGAGA TTCAGCGTGG GCGGCAGGCG 
CACGGAATGC GTAGTCGGGC CACGTCGATT GGAGCATCCT CGTCACCGGA GCACGGTGGC 
GGTCCGGCCT GGAAGCAACT GATCGGGCAG CTGGAGCATT TCTACAAACA GTTTCAGCAC 
TTCGGCTTGG ACAACTGCTA TGCGGAGCAG ATATTCCATC AACTGCTTTA CTTCATTTGC 
GCTGTGGCCC TTAATTGTCT GATGCTTAGG GGCGATATTT GCATGTGGGA GACTGGCATG 
ATAATCCGCT ACAATATCGG CTGCATTGAG GATTGGGTGC GCAGTAAAAA GATGTCTAAC 
GATGTGCTGA CAGCTTTGGC GCCTCTGAAT CAGGTCTCCC AATTGCTGCA GTCTCGGAAG 
AGCGAGCAGG ATGTTCAGAC CATTTGTGAT CTGTGTACTT CTCTGAGCAC GGCGCAGGTC 
CTCAAGGTGA TGAAGTCCTA CAAACTGGAC GATTATGAGA GCGAAATAAC GAACGTTTTT 
CTGGAGAAAC TAACCGAGAA ACTGAACGCC CGACAAATGC AAAAGAGCAA TAGTGACGAA 
TTCACCATAG ACCAGAAGTT CATTCAGCCA TTTAAGGTTG TCTTCAGGTA TAGTGACATC 
AAGCTGGAGG ATATTGAACT ACCGTCGCAT CTTAATCTGG ACGAGTTCCT TACAAAGATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724569.1
CDS	333..5735
Translation	MSSEEMLYAQGAKIWVPHADLVWESATLEESYRKGAGFLKICTDSGKLKEVKLKADGSDLPPLRNPAILVGQNDLTTLSYLHEPGVLHNLRVRFCERQIIYTYCGIILVAINPYAEMPLYGPSIIRAYRGHAMGDLEPHIFALAEEAYTKLERENCNLSIIVSGESGAGKTVSAKYAMRYFAAVGGSESETQVERKVLASSPIMEAFGNAKTTRNDNSSRFGKFTKLLFRNQMGVMFLQGATMHTYLLEKSRVVYQAQGERNYHIFYQLCAARSKYPELVLDHQDKFQFLNMGGAPEIERVSDAEQFNETVQAMTVLGFSIQQIADIVKILAGILHLGNIQVSKKFNEGSEEEDSDSCDIFHNDIHLQITADLLRVSADDLRRWLLMRKIESVNEYVLIPNSIEAAQAARDALAKHIYAKLFQYIVGVLNKSLNNGSKQCSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNQHVFKLEQEEYLKEGITWTMIDFYDNQPCIDLIESRLGVLDLLDEECRMPKGSDESWAGKLIGKCNKFPHFEKPRFGTTSFFIKHFSDTVEYDVNGFLEKNRDTVSKELTQVLSESNMSLAKQVMTLEEIDTLCVDSAKSSTLGGRVVISAGRKQQGNDTRRRVVPSKQHRKTVGSQFQESLASLISTLHATTPHYVRCIKPNDDKVAFKWETAKIIQQLRACGVLETVRISAAGFPSRWLYPDFYMRYQLLVYRSKLDKNDMKLSCRNIVMKWIQDEDKYRFGNTQIFFRAGQVAFLEQVRANLRKKYITIVQSVVRRFVYRRQFLRIQKVINGIQKHARGYLARERTQKMREARAGLILSKYARGWLCRRRYLRLRHSISGIQTYARGMLARNKFHAMRDHYRAVQIQRFVRGALARRAYQKRRRNIIICQAAIRRFLARRKFKRMKAEAKTISHMENKYMGLENKIISMQQRIDELNRDNSNLKHKTSEISVLKMKLELKKTLEAEFKNVKAACQDKDKLIEALNKQLEAERDEKMQLLEENGHAQEEWISQKQTWRQENEELRRQIDEIIDMAKNAEVNQRNQEDRMLAEIDNRELNEAYQRAIKDKEVIENENFMLKEELSRLTAGSFSLHARKASNASSQNEDDVGYASAKNTLDINRPPDLLSKNYSYNDSTSLVVKLRSILEEEKQKHKVLQEQYIKLSSRHKPTEDSFRVSELEVENEKLRSEYDQLRTSIKHGVEINELNAQHAALQEEVRRRREECIQLKAVLLQQSQSMRSLEPESLQMRGNDVNELMEAFHSQKLINRQLESELKAITEEHNSKLVEMTQEIERLNNEKDELQKVMFESIDEFEDSNVDTLRQNDRYLRRELQKAVAQFLLVQEELKLANAKLKAYRQDGGQLEHKIEEEMIRNKSNGTSADVGANVTKQKSQNPQGLMKFHSSDLDKILQRLLSALTPRTVVGLLPGFPAYLIFMCIRYTDLTNADDDVRELLSKFVIQIKKMHRTPHPIENRVIWLVNSITLLNLMKQYGDVDEYVKFNTEKQNQQQLKNFNLFEYRRVILDLIVNLYQALIMQIQGLLDPKIVPAILNNDEIQRGRQAHGMRSRATSIGASSSPEHGGGPAWKQLIGQLEHFYKQFQHFGLDNCYAEQIFHQLLYFICAVALNCLMLRGDICMWETGMIIRYNIGCIEDWVRSKKMSNDVLTALAPLNQVSQLLQSRKSEQDVQTICDLCTSLSTAQVLKVMKSYKLDDYESEITNVFLEKLTEKLNARQMQKSNSDEFTIDQKFIQPFKVVFRYSDIKLEDIELPSHLNLDEFLTKI

Target ORF information:

RefSeq Version	NM_165535.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dilute class unconventional myosin (didum), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165535.2

ORF Insert Sequence:

ATGTCTAGCG AGGAGATGCT ATACGCGCAG GGCGCCAAGA TCTGGGTGCC CCATGCGGAT 
CTGGTGTGGG AGAGCGCCAC CTTGGAGGAG AGCTACCGCA AGGGCGCCGG CTTCTTGAAG 
ATATGTACGG ACTCCGGAAA ACTGAAAGAG GTCAAGCTAA AGGCCGATGG CAGCGATCTG 
CCTCCACTGC GCAATCCGGC CATTCTGGTG GGACAGAACG ACTTGACCAC CCTGTCCTAC 
CTGCATGAGC CGGGGGTGTT GCACAATCTG CGTGTCCGCT TCTGCGAGCG CCAGATTATC 
TACACCTACT GCGGCATCAT TCTGGTGGCC ATCAACCCGT ACGCGGAGAT GCCTCTTTAC 
GGGCCCAGCA TAATCCGAGC GTATCGGGGT CATGCTATGG GTGATCTGGA GCCGCACATC 
TTTGCCCTGG CGGAGGAGGC GTACACGAAA CTGGAGCGCG AGAACTGCAA CCTGAGCATC 
ATCGTCAGTG GGGAGTCGGG TGCGGGCAAG ACGGTGTCCG CCAAATACGC CATGAGGTAC 
TTTGCCGCTG TTGGAGGTTC CGAGTCGGAG ACCCAGGTCG AACGCAAGGT GCTGGCATCT 
TCGCCGATCA TGGAAGCCTT CGGAAATGCC AAGACGACCC GGAATGACAA CAGTTCCCGC 
TTTGGAAAGT TTACCAAGCT GCTGTTCCGG AACCAGATGG GTGTGATGTT CCTGCAGGGA 
GCCACTATGC ACACCTACCT ACTGGAGAAG TCACGTGTGG TGTACCAGGC CCAGGGAGAG 
CGCAACTATC ACATATTCTA TCAGCTGTGC GCGGCGCGAT CGAAGTACCC TGAACTGGTG 
CTGGATCACC AGGACAAATT CCAGTTTCTG AACATGGGTG GCGCTCCTGA AATTGAACGA 
GTTTCGGATG CGGAGCAGTT TAACGAAACC GTGCAGGCCA TGACAGTTCT GGGCTTCTCC 
ATTCAACAGA TCGCTGATAT CGTAAAGATC CTGGCAGGAA TACTCCATTT AGGAAACATT 
CAGGTTTCCA AGAAGTTCAA CGAGGGCAGC GAAGAGGAGG ACAGTGACTC TTGCGATATA 
TTTCATAACG ACATCCACCT GCAGATCACC GCCGATCTAC TGCGGGTGAG CGCCGATGAT 
CTGCGCCGGT GGCTTTTGAT GCGTAAGATA GAGTCGGTCA ATGAATATGT GCTGATACCG 
AATAGCATTG AGGCGGCTCA GGCGGCTCGA GACGCTCTGG CCAAGCACAT CTATGCGAAA 
CTGTTTCAGT ATATAGTCGG TGTGCTGAAC AAGAGCCTCA ACAACGGTAG CAAGCAGTGC 
AGCTTCATTG GCGTCCTCGA TATCTACGGC TTCGAAACGT TCGAGGTGAA CTCCTTTGAA 
CAATTTTGCA TAAACTATGC GAACGAAAAG CTTCAGCAGC AGTTCAACCA GCATGTCTTC 
AAGCTGGAGC AGGAGGAGTA TCTTAAGGAA GGAATCACCT GGACGATGAT TGACTTTTAC 
GACAATCAAC CGTGTATCGA TCTAATTGAA TCTCGCTTGG GAGTGCTGGA CCTGCTCGAC 
GAGGAGTGTC GAATGCCAAA GGGCTCGGAC GAGAGCTGGG CTGGCAAGCT CATCGGAAAG 
TGCAATAAAT TTCCGCATTT CGAGAAGCCA CGCTTTGGCA CAACCAGCTT CTTTATCAAA 
CATTTCTCGG ACACGGTCGA GTATGACGTG AACGGATTCT TGGAAAAGAA TCGTGACACA 
GTCTCCAAGG AGTTGACGCA AGTGCTAAGC GAGTCCAACA TGTCTCTGGC CAAGCAGGTG 
ATGACCCTGG AGGAAATAGA TACTCTGTGC GTGGATTCCG CTAAATCCTC CACCTTGGGC 
GGCCGCGTCG TGATCAGTGC TGGCCGCAAA CAGCAAGGGA ATGACACACG TCGAAGAGTG 
GTGCCATCCA AGCAGCATAG AAAAACGGTG GGATCGCAGT TCCAGGAGAG TCTGGCGTCG 
CTGATATCTA CGTTACATGC CACAACTCCG CACTATGTGC GCTGCATCAA GCCCAACGAT 
GACAAAGTCG CCTTTAAGTG GGAGACGGCC AAGATCATAC AGCAGTTAAG GGCCTGTGGT 
GTGCTGGAAA CGGTGCGCAT CTCCGCAGCG GGATTCCCCT CGAGATGGCT CTATCCCGAC 
TTCTATATGC GGTACCAGCT GCTGGTTTAC CGCTCCAAAC TCGACAAAAA CGACATGAAG 
CTGTCGTGCC GGAACATTGT GATGAAGTGG ATCCAAGACG AAGATAAGTA CCGATTTGGC 
AACACGCAGA TTTTCTTCCG CGCCGGCCAA GTGGCCTTCC TTGAACAGGT TCGGGCTAAT 
CTGCGCAAGA AGTATATCAC CATTGTGCAG TCGGTTGTGC GGCGATTCGT CTACCGGCGC 
CAGTTCCTGC GCATTCAGAA AGTAATTAAT GGCATTCAGA AACATGCGCG CGGATATCTT 
GCTCGCGAGC GTACTCAGAA AATGCGCGAA GCTCGTGCGG GATTAATCCT GTCGAAGTAC 
GCCCGCGGTT GGTTATGCCG TCGTCGTTAC TTGCGCCTAC GCCACTCCAT TTCCGGCATA 
CAGACCTACG CCCGCGGCAT GCTGGCGCGC AACAAGTTCC ACGCGATGCG GGATCACTAC 
CGGGCAGTTC AGATCCAGCG TTTCGTGCGT GGTGCTTTGG CACGGCGAGC TTACCAAAAG 
CGGCGTCGCA ACATCATCAT TTGTCAAGCG GCGATTCGGA GATTCTTGGC CCGTCGTAAG 
TTTAAACGCA TGAAGGCCGA GGCCAAGACC ATCTCGCACA TGGAAAACAA ATACATGGGG 
CTGGAAAACA AGATTATTTC CATGCAGCAG CGGATCGATG AGCTGAATCG CGACAACAGT 
AATCTGAAGC ACAAGACCAG CGAAATCAGT GTATTGAAAA TGAAGCTTGA GCTGAAGAAG 
ACCCTGGAGG CTGAATTCAA AAATGTCAAG GCCGCCTGCC AGGACAAGGA CAAGCTGATC 
GAAGCACTTA ACAAGCAGTT GGAGGCGGAG CGAGACGAAA AAATGCAGTT GCTGGAGGAG 
AACGGACATG CTCAAGAGGA GTGGATCAGC CAGAAGCAGA CGTGGCGCCA AGAGAACGAG 
GAGCTGCGCC GTCAGATAGA CGAGATAATC GATATGGCAA AGAACGCAGA AGTCAACCAG 
CGTAACCAGG AGGACCGAAT GCTAGCCGAG ATTGATAACA GGGAGCTCAA CGAGGCCTAC 
CAACGAGCTA TTAAGGACAA GGAGGTCATC GAGAACGAAA ACTTCATGCT GAAGGAAGAG 
CTCAGTCGAT TAACGGCTGG CAGTTTCAGT TTGCACGCCC GCAAGGCTAG CAACGCCTCC 
AGCCAAAACG AGGACGATGT GGGATACGCC TCCGCCAAGA ACACTCTGGA TATCAATCGG 
CCCCCGGATT TGTTAAGCAA AAATTACTCG TACAATGACT CTACCAGTCT GGTGGTGAAG 
TTGAGATCCA TTCTCGAGGA GGAGAAGCAA AAGCACAAGG TCTTGCAGGA GCAGTACATT 
AAGTTGTCCA GTCGGCATAA GCCCACCGAG GATTCCTTCC GCGTCTCCGA GCTTGAGGTA 
GAGAATGAAA AGCTGCGCAG CGAGTACGAT CAGCTGCGAA CGAGCATTAA ACACGGTGTT 
GAGATCAACG AGCTCAATGC ACAGCATGCC GCCTTGCAGG AAGAGGTACG TAGGCGGCGC 
GAGGAGTGCA TCCAATTAAA GGCAGTCCTG CTGCAGCAGA GCCAGTCCAT GAGATCGCTC 
GAGCCGGAAA GTCTACAGAT GCGTGGCAAC GATGTCAACG AACTGATGGA AGCCTTCCAT 
TCCCAGAAGC TAATTAATCG TCAATTGGAG TCTGAGCTCA AGGCCATCAC CGAGGAGCAC 
AACAGTAAGC TCGTGGAGAT GACACAGGAG ATCGAGAGAT TGAACAATGA GAAGGATGAG 
CTGCAAAAGG TAATGTTCGA GAGCATCGAC GAGTTCGAAG ATTCCAATGT GGATACGCTG 
AGACAGAACG ATCGCTATCT GCGACGAGAA CTGCAGAAGG CTGTAGCCCA GTTCCTGCTC 
GTTCAGGAGG AGCTCAAACT GGCAAATGCC AAGCTTAAAG CTTATCGGCA GGATGGAGGC 
CAGCTGGAGC ACAAGATAGA GGAGGAGATG ATTCGCAACA AGTCCAACGG AACGTCCGCC 
GATGTAGGCG CGAATGTGAC GAAGCAAAAG TCTCAGAATC CGCAAGGGCT GATGAAGTTC 
CACAGCAGCG ATCTGGACAA GATCTTGCAA CGCCTGCTTA GCGCCTTGAC TCCACGCACA 
GTGGTCGGGC TCCTTCCTGG TTTTCCAGCA TATCTCATCT TTATGTGTAT TCGATACACC 
GATCTGACAA ATGCCGACGA TGATGTGCGC GAGTTGCTAA GCAAGTTCGT TATTCAGATT 
AAGAAAATGC ATCGTACGCC GCATCCGATC GAGAATCGTG TTATTTGGCT CGTCAATTCC 
ATTACGCTGC TAAATCTTAT GAAGCAATAC GGCGACGTGG ATGAGTACGT CAAGTTCAAT 
ACTGAGAAGC AGAATCAGCA GCAGCTGAAG AACTTCAATC TCTTTGAATA CCGTCGCGTA 
ATTCTTGATT TAATTGTGAA CCTGTACCAG GCGCTGATCA TGCAGATCCA GGGTCTGTTG 
GACCCAAAAA TAGTGCCAGC GATTCTCAAC AATGATGAGA TTCAGCGTGG GCGGCAGGCG 
CACGGAATGC GTAGTCGGGC CACGTCGATT GGAGCATCCT CGTCACCGGA GCACGGTGGC 
GGTCCGGCCT GGAAGCAACT GATCGGGCAG CTGGAGCATT TCTACAAACA GTTTCAGCAC 
TTCGGCTTGG ACAACTGCTA TGCGGAGCAG ATATTCCATC AACTGCTTTA CTTCATTTGC 
GCTGTGGCCC TTAATTGTCT GATGCTTAGG GGCGATATTT GCATGTGGGA GACTGGCATG 
ATAATCCGCT ACAATATCGG CTGCATTGAG GATTGGGTGC GCAGTAAAAA GATGTCTAAC 
GATGTGCTGA CAGCTTTGGC GCCTCTGAAT CAGGTCTCCC AATTGCTGCA GTCTCGGAAG 
AGCGAGCAGG ATGTTCAGAC CATTTGTGAT CTGTGTACTT CTCTGAGCAC GGCGCAGGTC 
CTCAAGGTGA TGAAGTCCTA CAAACTGGAC GATTATGAGA GCGAAATAAC GAACGTTTTT 
CTGGAGAAAC TAACCGAGAA ACTGAACGCC CGACAAATGC AAAAGAGCAA TAGTGACGAA 
TTCACCATAG ACCAGAAGTT CATTCAGCCA TTTAAGGTTG TCTTCAGGTA TAGTGACATC 
AAGCTGGAGG ATATTGAACT ACCGTCGCAT CTTAATCTGG ACGAGTTCCT TACAAAGATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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didum cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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