myo5a gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004912695.3	XP_004912752.1	unconventional myosin-Va isoform X1
XM_004912697.3	XP_004912754.1	unconventional myosin-Va isoform X4
XM_018092427.1	XP_017947916.1	unconventional myosin-Va isoform X5
XM_031899180.1	XP_031755040.1	unconventional myosin-Va isoform X1
XM_031899183.1	XP_031755043.1	unconventional myosin-Va isoform X4
XM_031899189.1	XP_031755049.1	unconventional myosin-Va isoform X10
XM_002936347.4	XP_002936393.2	unconventional myosin-Va isoform X3
XM_031899182.1	XP_031755042.1	unconventional myosin-Va isoform X3
XM_031899185.1	XP_031755045.1	unconventional myosin-Va isoform X6
XM_031899186.1	XP_031755046.1	unconventional myosin-Va isoform X7
XM_031899187.1	XP_031755047.1	unconventional myosin-Va isoform X8
XM_004912697.4	XP_004912754.2	unconventional myosin-Va isoform X11
XM_031899191.1	XP_031755051.1	unconventional myosin-Va isoform X13
XM_004912696.3	XP_004912753.1	unconventional myosin-Va isoform X2
XM_031899190.1	XP_031755050.1	unconventional myosin-Va isoform X12
XM_002936347.5	XP_002936393.3	unconventional myosin-Va isoform X14
XM_031899184.1	XP_031755044.1	unconventional myosin-Va isoform X5
XM_031899181.1	XP_031755041.1	unconventional myosin-Va isoform X2
XM_031899188.1	XP_031755048.1	unconventional myosin-Va isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa43363	XM_018092427.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa55089	XM_031899180.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55090	XM_031899181.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55091	XM_031899182.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55092	XM_031899183.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55093	XM_031899184.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55094	XM_031899185.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55095	XM_031899186.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55096	XM_031899187.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55097	XM_031899188.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55098	XM_031899189.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55099	XM_004912697.4 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55100	XM_031899190.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55101	XM_031899191.1 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55102	XM_002936347.5 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa07363	XM_004912695.3 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa07364	XM_004912696.3 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa07365	XM_002936347.4 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa07366	XM_004912697.3 Latest version!	Xenopus tropicalis myosin VA (myo5a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

myo5a ( XM_018092427.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947916.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa43363
Clone ID Related Accession (Same CDS sequence)	XM_018092427.1
Accession Version	XM_018092427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTTATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC AGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGGA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACG GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAGA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ATGACAAGGA CACAATGACC GATTCCATGG TCCTCTCTGA GGATGTACAG 
AAGATGAAGG ATAAAAGGGA AATTGCTCAG GCCTACACTG GCATGAAGGA GACAAATAGA 
AACCTGCCTC CAGACTACCA AGACCTAAAT GAGGACGGAG AGCTGTGGCT GGTTTATGAT 
GGGTTAAAAC AGGCCAACAG GGTTTTGGAA TCCCAGCTGC AATCACAGAA AAAGGCCTTT 
GAGAATGAAG TGGAAGCTCT CAGAGGTGAG ATCCAGGCTC TAAAAGAGGA GAACAACCGG 
CAGCAGCAAC TTTTAGCCCA GAACCTCCAG TTGCCACCTG AAGCACGAAT CGAAGCCAGC 
TTGCAGCATG AAATCACCAG GCTAACCAAC GAGAACTTGG ATCTGATGGA GCAACTTGAA 
AAGCAAGATA AGACTGTCCG CAAACTGAAA AAGCAGTTGA AAGTCTTTGC CAAGAAAATT 
GGAGAGCTGG AAGTTGGTCA AATGGAAAAT GTTTCTCCTG GTCAGATAGT GGATGAACCC 
ATTCGTCCTG TTAATATTCC GCGCAAAGAG AAGGACTTCC AAGGAATGCT GGAATATAAG 
AAAGAGGATG AACTAAAGCT TGTCAAAAAC CTCATTTTAG AACTGAAGCC TCGTGGTGTC 
GCGGTCAACC TCATTCCAGG ACTGCCAGCA TATATCTTGT TCATGTGTTT GCGTCATGCT 
GATTACCTCA ATGATGATCA GAAAGTTCGC TCCTTGCTTA CATCAACTAT TAATGGTATC 
AAGAAAATCT TAAAGAAAAG AGGAGATGAT TTTGAAACGG TGTCCTTCTG GCTGTCCAAT 
TCGTGTCGGT TCCTCCATTG CTTGAAGCAG TACAGTGGAG AAGAGGGATT TATGAAGCAC 
AACACCACAC GGCAGAATGA GCACTGTCTG ACCAACTTTG ACCTTGCGGA ATACAGACAA 
GTCTTGAGTG ATCTGGCTAT TCAGATTTAC CAGCAGTTGG TCAGAGTGTT GGAAAATATC 
TTGCAGCCAA TGATTGTTTC AGGAATGCTG GAGCACGAGA CTATTCAGGG TGTCTCAGGG 
GTCAAGCCTA CCGGTCTTAG GAAGAGAACT TCCAGCATAG CAGATGAAGG GACGTACACA 
TTAGACTCCA TTGTGAGGCA GCTTAATACC TTCCATTCCA TAATGTGTCA GCATGGGATG 
GATCCTGAAC TCATCAAGCA GGTGGTCAAG CAAATGTTCT ACATCATCGG TGCTGTCACA 
CTCAACAACT TGCTCTTGCG AAAGGACATG TGCTCTTGGA GCAAAGGCAT GCAGATAAGG 
TACAACGTGA GCCAGTTGGA AGAATGGCTG CGTGACAAGA ACCTGATGAA TAGCAGCGCT 
AAAGAAACCC TGGAGCCATT AATTCAGGCT GCTCAGTTAC TGCAGGTGAA AAAGAAAACA 
GATGAAGATG CAGAAGCCAT CTGCTCCATG TGCAATGCTC TGACTACTGC CCAGATTGTC 
AAGGTGTTGA ACCTGTACAC TCCAGTAAAT GAGTTTGAAG AGCGGGTGTT GGTATCATTT 
ATACGGAATA TACAGATGCG TTTAAGGGAT AGGAAGGACT CCCCTCAGCT GCTAATGGAT 
GCTAAGCATA TCTTCCCTGT CACCTTCCCA TTCAACCCTT CGTCGCTTGC CCTAGAAACA 
ATTCAGATTC CTGGCAGTCT TGGCCTTGCC TTCCTCACAC GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947916.1
CDS	1036..6603
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTRAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDDKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNRNLPPDYQDLNEDGELWLVYDGLKQANRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_018092427.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092427.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTTATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC AGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGGA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACG GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAGA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ATGACAAGGA CACAATGACC GATTCCATGG TCCTCTCTGA GGATGTACAG 
AAGATGAAGG ATAAAAGGGA AATTGCTCAG GCCTACACTG GCATGAAGGA GACAAATAGA 
AACCTGCCTC CAGACTACCA AGACCTAAAT GAGGACGGAG AGCTGTGGCT GGTTTATGAT 
GGGTTAAAAC AGGCCAACAG GGTTTTGGAA TCCCAGCTGC AATCACAGAA AAAGGCCTTT 
GAGAATGAAG TGGAAGCTCT CAGAGGTGAG ATCCAGGCTC TAAAAGAGGA GAACAACCGG 
CAGCAGCAAC TTTTAGCCCA GAACCTCCAG TTGCCACCTG AAGCACGAAT CGAAGCCAGC 
TTGCAGCATG AAATCACCAG GCTAACCAAC GAGAACTTGG ATCTGATGGA GCAACTTGAA 
AAGCAAGATA AGACTGTCCG CAAACTGAAA AAGCAGTTGA AAGTCTTTGC CAAGAAAATT 
GGAGAGCTGG AAGTTGGTCA AATGGAAAAT GTTTCTCCTG GTCAGATAGT GGATGAACCC 
ATTCGTCCTG TTAATATTCC GCGCAAAGAG AAGGACTTCC AAGGAATGCT GGAATATAAG 
AAAGAGGATG AACTAAAGCT TGTCAAAAAC CTCATTTTAG AACTGAAGCC TCGTGGTGTC 
GCGGTCAACC TCATTCCAGG ACTGCCAGCA TATATCTTGT TCATGTGTTT GCGTCATGCT 
GATTACCTCA ATGATGATCA GAAAGTTCGC TCCTTGCTTA CATCAACTAT TAATGGTATC 
AAGAAAATCT TAAAGAAAAG AGGAGATGAT TTTGAAACGG TGTCCTTCTG GCTGTCCAAT 
TCGTGTCGGT TCCTCCATTG CTTGAAGCAG TACAGTGGAG AAGAGGGATT TATGAAGCAC 
AACACCACAC GGCAGAATGA GCACTGTCTG ACCAACTTTG ACCTTGCGGA ATACAGACAA 
GTCTTGAGTG ATCTGGCTAT TCAGATTTAC CAGCAGTTGG TCAGAGTGTT GGAAAATATC 
TTGCAGCCAA TGATTGTTTC AGGAATGCTG GAGCACGAGA CTATTCAGGG TGTCTCAGGG 
GTCAAGCCTA CCGGTCTTAG GAAGAGAACT TCCAGCATAG CAGATGAAGG GACGTACACA 
TTAGACTCCA TTGTGAGGCA GCTTAATACC TTCCATTCCA TAATGTGTCA GCATGGGATG 
GATCCTGAAC TCATCAAGCA GGTGGTCAAG CAAATGTTCT ACATCATCGG TGCTGTCACA 
CTCAACAACT TGCTCTTGCG AAAGGACATG TGCTCTTGGA GCAAAGGCAT GCAGATAAGG 
TACAACGTGA GCCAGTTGGA AGAATGGCTG CGTGACAAGA ACCTGATGAA TAGCAGCGCT 
AAAGAAACCC TGGAGCCATT AATTCAGGCT GCTCAGTTAC TGCAGGTGAA AAAGAAAACA 
GATGAAGATG CAGAAGCCAT CTGCTCCATG TGCAATGCTC TGACTACTGC CCAGATTGTC 
AAGGTGTTGA ACCTGTACAC TCCAGTAAAT GAGTTTGAAG AGCGGGTGTT GGTATCATTT 
ATACGGAATA TACAGATGCG TTTAAGGGAT AGGAAGGACT CCCCTCAGCT GCTAATGGAT 
GCTAAGCATA TCTTCCCTGT CACCTTCCCA TTCAACCCTT CGTCGCTTGC CCTAGAAACA 
ATTCAGATTC CTGGCAGTCT TGGCCTTGCC TTCCTCACAC GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_031899180.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755040.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X1, mRNA.

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CloneID	OXa55089
Clone ID Related Accession (Same CDS sequence)	XM_031899180.1
Accession Version	XM_031899180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGATGA CAAGGACACA ATGACCGATT CCATGGTCCT CTCTGAGGAT 
GTACAGAAGA TGAAGGATAA AAGGGAAATT GCTCAGGCCT ACACTGGCAT GAAGGAGACA 
AATAGTCTTG AAACTATTGA AAGAAACCTG CCTCCAGACT ACCAAGCCCT AAATGAGGAC 
GGAGAGCTGT GGCTGGTTTA TGATGGGTTA AAACAGGCCA ACAGGGTTTT GGAATCCCAG 
CTGCAATCAC AGAAAAAGGC CTTTGAGAAT GAAGTGGAAG CTCTCAGAGG TGAGATCCAG 
GCTCTAAAAG AGGAGAACAA CCGGCAGCAG CAACTTTTAG CCCAGAACCT CCAGTTGCCA 
CCTGAAGCAC GAATCGAAGC CAGCTTGCAG CATGAAATCA CCAGGCTAAC CAACGAGAAC 
TTGTTTTTTG AGGAGTTATA TGCAGATGAA CCCATGAAGT ATCAATCGTA CCGGATCTCC 
CTGTACAGAC GGATCATTGA TCTGATGGAG CAACTTGAAA AGCAAGATAA GACTGTCCGC 
AAACTGAAAA AGCAGTTGAA AGTCTTTGCC AAGAAAATTG GAGAGCTGGA AGTTGGTCAA 
ATGGAAAATG TTTCTCCTGG TCAGATAGTG GATGAACCCA TTCGTCCTGT TAATATTCCG 
CGCAAAGAGA AGGACTTCCA AGGAATGCTG GAATATAAGA AAGAGGACGA ACTAAAGCTT 
GTCAAAAACC TCATTTTAGA ACTGAAGCCT CGTGGTGTCG CGGTCAACCT CATTCCAGGA 
CTGCCAGCAT ATATCTTGTT CATGTGTTTG CGTCATGCTG ATTACCTCAA TGATGATCAG 
AAAGTTCGCT CCTTGCTTAC ATCAACTATT AATGGTATCA AGAAAATCTT AAAGAAAAGA 
GGAGATGATT TTGAAACGGT GTCCTTCTGG CTGTCCAATT CGTGTCGGTT CCTCCATTGC 
TTGAAGCAGT ACAGTGGAGA AGAGGGATTT ATGAAGCACA ACACCACACG GCAGAATGAG 
CACTGTCTGA CCAACTTTGA CCTTGCGGAA TACAGACAAG TCTTGAGTGA TCTGGCTATT 
CAGATTTACC AGCAGTTGGT CAGAGTGTTG GAAAATATCT TGCAGCCAAT GATTGTTTCA 
GGAATGCTGG AGCACGAGAC TATTCAGGGT GTCTCAGGGG TCAAGCCTAC CGGTCTTAGG 
AAGAGAACTT CCAGCATAGC AGATGAAGGG ACGTACACAT TAGACTCCAT TGTGAGGCAG 
CTTAATACCT TCCATTCCAT AATGTGTCAG CATGGGATGG ATCCTGAACT CATCAAGCAG 
GTGGTCAAGC AAATGTTCTA CATCATCGGT GCTGTCACAC TCAACAACTT GCTCTTGCGA 
AAGGACATGT GCTCTTGGAG CAAAGGCATG CAGATAAGGT ACAACGTGAG CCAGTTGGAA 
GAATGGCTGC GTGACAAGAA CCTGATGAAT AGCAGCGCTA AAGAAACCCT GGAGCCATTA 
ATTCAGGCTG CTCAGTTACT GCAGGTGAAA AAGAAAACAG ACGAAGATGC AGAAGCCATC 
TGCTCCATGT GCAATGCTCT GACTACTGCC CAGATTGTCA AGGTGTTGAA CCTGTACACT 
CCAGTAAATG AGTTTGAAGA GCGGGTGTTG GTATCATTTA TACGGAATAT ACAGATGCGT 
TTAAGGGATA GGAAGGACTC CCCTCAGCTG CTAATGGATG CTAAGCATAT CTTCCCTGTC 
ACCTTCCCAT TCAACCCTTC GTCGCTTGCC CTAGAAACAA TTCAGATTCC TGGCAGTCTT 
GGCCTTGCCT TCCTCACACG TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755040.1
CDS	354..6020
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKKKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDDKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNSLETIERNLPPDYQALNEDGELWLVYDGLKQANRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLFFEELYADEPMKYQSYRISLYRRIIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_031899180.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899180.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGATGA CAAGGACACA ATGACCGATT CCATGGTCCT CTCTGAGGAT 
GTACAGAAGA TGAAGGATAA AAGGGAAATT GCTCAGGCCT ACACTGGCAT GAAGGAGACA 
AATAGTCTTG AAACTATTGA AAGAAACCTG CCTCCAGACT ACCAAGCCCT AAATGAGGAC 
GGAGAGCTGT GGCTGGTTTA TGATGGGTTA AAACAGGCCA ACAGGGTTTT GGAATCCCAG 
CTGCAATCAC AGAAAAAGGC CTTTGAGAAT GAAGTGGAAG CTCTCAGAGG TGAGATCCAG 
GCTCTAAAAG AGGAGAACAA CCGGCAGCAG CAACTTTTAG CCCAGAACCT CCAGTTGCCA 
CCTGAAGCAC GAATCGAAGC CAGCTTGCAG CATGAAATCA CCAGGCTAAC CAACGAGAAC 
TTGTTTTTTG AGGAGTTATA TGCAGATGAA CCCATGAAGT ATCAATCGTA CCGGATCTCC 
CTGTACAGAC GGATCATTGA TCTGATGGAG CAACTTGAAA AGCAAGATAA GACTGTCCGC 
AAACTGAAAA AGCAGTTGAA AGTCTTTGCC AAGAAAATTG GAGAGCTGGA AGTTGGTCAA 
ATGGAAAATG TTTCTCCTGG TCAGATAGTG GATGAACCCA TTCGTCCTGT TAATATTCCG 
CGCAAAGAGA AGGACTTCCA AGGAATGCTG GAATATAAGA AAGAGGACGA ACTAAAGCTT 
GTCAAAAACC TCATTTTAGA ACTGAAGCCT CGTGGTGTCG CGGTCAACCT CATTCCAGGA 
CTGCCAGCAT ATATCTTGTT CATGTGTTTG CGTCATGCTG ATTACCTCAA TGATGATCAG 
AAAGTTCGCT CCTTGCTTAC ATCAACTATT AATGGTATCA AGAAAATCTT AAAGAAAAGA 
GGAGATGATT TTGAAACGGT GTCCTTCTGG CTGTCCAATT CGTGTCGGTT CCTCCATTGC 
TTGAAGCAGT ACAGTGGAGA AGAGGGATTT ATGAAGCACA ACACCACACG GCAGAATGAG 
CACTGTCTGA CCAACTTTGA CCTTGCGGAA TACAGACAAG TCTTGAGTGA TCTGGCTATT 
CAGATTTACC AGCAGTTGGT CAGAGTGTTG GAAAATATCT TGCAGCCAAT GATTGTTTCA 
GGAATGCTGG AGCACGAGAC TATTCAGGGT GTCTCAGGGG TCAAGCCTAC CGGTCTTAGG 
AAGAGAACTT CCAGCATAGC AGATGAAGGG ACGTACACAT TAGACTCCAT TGTGAGGCAG 
CTTAATACCT TCCATTCCAT AATGTGTCAG CATGGGATGG ATCCTGAACT CATCAAGCAG 
GTGGTCAAGC AAATGTTCTA CATCATCGGT GCTGTCACAC TCAACAACTT GCTCTTGCGA 
AAGGACATGT GCTCTTGGAG CAAAGGCATG CAGATAAGGT ACAACGTGAG CCAGTTGGAA 
GAATGGCTGC GTGACAAGAA CCTGATGAAT AGCAGCGCTA AAGAAACCCT GGAGCCATTA 
ATTCAGGCTG CTCAGTTACT GCAGGTGAAA AAGAAAACAG ACGAAGATGC AGAAGCCATC 
TGCTCCATGT GCAATGCTCT GACTACTGCC CAGATTGTCA AGGTGTTGAA CCTGTACACT 
CCAGTAAATG AGTTTGAAGA GCGGGTGTTG GTATCATTTA TACGGAATAT ACAGATGCGT 
TTAAGGGATA GGAAGGACTC CCCTCAGCTG CTAATGGATG CTAAGCATAT CTTCCCTGTC 
ACCTTCCCAT TCAACCCTTC GTCGCTTGCC CTAGAAACAA TTCAGATTCC TGGCAGTCTT 
GGCCTTGCCT TCCTCACACG TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_031899181.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755041.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X2, mRNA.

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CloneID	OXa55090
Clone ID Related Accession (Same CDS sequence)	XM_031899181.1
Accession Version	XM_031899181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGTTTAC ACTGCTAACA GAACTGTTCC AAGAAGAGGA GCAGATCTTG 
AGCCCTACAT CTTCTGCACC ACCCTCAGGC CGTACTCTGC TATCCCGCAC TGGTCTGCGT 
TCCCTCAAGC CCAAGCCTGA ACAGACAAGC AAGGAGCATA AAAAAACTGT AGGACATCAG 
TTCAGAAATT CCCTTCACCT TCTGATGGAG ACTCTCAATG CCACAACTCC ACATTATGTC 
CGTTGCATTA AGCCAAATGA CTTCAAGTAC CCGTTCACAT TTGACTCTAA ACGAGCAGTG 
CAGCAGCTTA GGGCTTGTGG CGTCCTGGAA ACCATCCGAA TCAGTGCTGC TGGATTTCCT 
TCTAGGTGGA CATATCAGGA ATTTTTTAGC AGATATCGTG TTTTAATGAA GCAAAAAGAT 
GTCCTCAGTG ATAGGAAGCA AACCTGCAAA AATGTTCTGG AGAAGCTTAT TTTGGATAAG 
GATAAGTACC AGTTTGGTAA GACAAAGATT TTTTTCCGTG CTGGCCAAGT GGCCTATCTG 
GAGAAGATCC GAGCAGACAA GCTGAGAATG GCCTGTATCA GGATCCAGAA GACAATCCGA 
GGATGGCTTT TGAGGAAGAA ATACTTGCGC ATGAGGAAAG CAGCCATAAC AATCCAGCGC 
TACGTCAGAG GATACCAGGC ACGATGTTAT GCACAGTTTC TGCGTAGAAC ACAGGCCGCG 
ATTATTATCC AGAAATTCCA AAGGATGTAT GTCGTTCGGC AAAAGTACCG TCATATGCAG 
TCCATTACCC TTGCTCTCCA GTCGTATATG CGTGGTTATG CAGCCAGGAA GAGATTTCAG 
GGGATGCTTA GAGCACACAA AGCTACCATC ATCCAGAAGC ATGTGCGCGG CTGGTTAGCC 
CGTGTCACTT ATAAGAGAAA TTTGAGTGCT ATTGTGTACC TGCAGTGTTG TTACCGCAGG 
ATGATGGCCA AAAGGGAGCT GAAGAAGTTA AAGATAGAGG CACGCTCAGT GGAACACTAC 
AAGAAACTCA ACAATGGGAT GGAAAACAAG ATCATGCAGT TGCAGCGTAA AGTTGATGAG 
CAGAATAAAG ACAACAAATC CTTGCTGGAG AGGCTAACAC ACCTGGAGGT CACGTACAAT 
ACTGAAAAAG ATAAGCTGAG AAGCGACGTC GACAGACTGC GCCATTTTGA AGAGGAGGCC 
AAAAATGCAG CAAACAGGAT GGTCAGCTTG CAGGACGAGC TTGCCAGGCT TAGGAAAGAG 
CTGCAGCAAA CACAGACGGA AAAGAACAAG ATTGAGGAAC GGGCAGAGAA GTACCAGACC 
GAGACTGAAA AGCTGGTTGC TGAGCTGAGA GAACAGAATG CTTTGCTGAA AGCTGAGAAA 
GAGGAACTGA ATCTTTTAAT CCAGGAGCAG GCAAAAAAAA TGACAGAGGA TATGGAAAAG 
AAAATTATAG AAGAAACAAA GCAGTTGGAG CTTGAGCTGA ATGATGAACG ACTGAGGTAC 
CAGAATCTCC TAAACGAGTA CAGCCGCCTG GAGGAGCGAT ATGATGACCT TAAAGATGAA 
ATTAATACCA TGAGTCCTCC AAAGCCAGGA CACAAGCGCA CAGACTCTAC TCATAGCAGC 
AATGAATCAG AATACACTTT TAGTTCAGAG ATCACAGAGT CAGAAGATTA CCCATATCGA 
AATGAGGAGC CTGCTGACAG AAAAACTCCG CTGGACATGT CCCTGTTCCT TAAGCTTCAG 
AAACGGGTAA AGGAACTTGA GCAGGAAAAG CAAGTAATGC AAGATGATTT GGATAAGAAG 
GAGGAGCAGA TAATCCGAGC CAAAATAATG GAGGAGGCAA AGACTCCAAC TAGAGGTACA 
GAACTGGAAT ATGAATCACT CAAGCGTCAA GAACTGGAAT CTGAAAATAA AAAACTTAAG 
AATGAGTTAA ATGAGCTGCG AAGGGCAATC ACTGAGAAAG CCTCCCCAGA TGCTACAGGC 
CCTGGTGGAA CTGTCTATAA AGTTCTCTTG GAGCAGATGA CGTCAGTAAG TGAGGAACTT 
GAAGTACGCA AGGAAGAAGT CCTTATACTG AGGTCCCAGT TGGTGAGCCA AATGGAAGCC 
ATTCCACACA AGGATGACAA GGACACAATG ACCGATTCCA TGGTCCTCTC TGAGGATGTA 
CAGAAGATGA AGGATAAAAG GGAAATTGCT CAGGCCTACA CTGGCATGAA GGAGACAAAT 
AGTCTTGAAA CTATTGAAAG AAACCTGCCT CCAGACTACC AAGCCCTAAA TGAGGACGGA 
GAGCTGTGGC TGGTTTATGA TGGGTTAAAA CAGGCCAACA GGGTTTTGGA ATCCCAGCTG 
CAATCACAGA AAAAGGCCTT TGAGAATGAA GTGGAAGCTC TCAGAGGTGA GATCCAGGCT 
CTAAAAGAGG AGAACAACCG GCAGCAGCAA CTTTTAGCCC AGAACCTCCA GTTGCCACCT 
GAAGCACGAA TCGAAGCCAG CTTGCAGCAT GAAATCACCA GGCTAACCAA CGAGAACTTG 
TTTTTTGAGG AGTTATATGC AGATGAACCC ATGAAGTATC AATCGTACCG GATCTCCCTG 
TACAGACGGA TCATTGATCT GATGGAGCAA CTTGAAAAGC AAGATAAGAC TGTCCGCAAA 
CTGAAAAAGC AGTTGAAAGT CTTTGCCAAG AAAATTGGAG AGCTGGAAGT TGGTCAAATG 
GAAAATGTTT CTCCTGGTCA GATAGTGGAT GAACCCATTC GTCCTGTTAA TATTCCGCGC 
AAAGAGAAGG ACTTCCAAGG AATGCTGGAA TATAAGAAAG AGGACGAACT AAAGCTTGTC 
AAAAACCTCA TTTTAGAACT GAAGCCTCGT GGTGTCGCGG TCAACCTCAT TCCAGGACTG 
CCAGCATATA TCTTGTTCAT GTGTTTGCGT CATGCTGATT ACCTCAATGA TGATCAGAAA 
GTTCGCTCCT TGCTTACATC AACTATTAAT GGTATCAAGA AAATCTTAAA GAAAAGAGGA 
GATGATTTTG AAACGGTGTC CTTCTGGCTG TCCAATTCGT GTCGGTTCCT CCATTGCTTG 
AAGCAGTACA GTGGAGAAGA GGGATTTATG AAGCACAACA CCACACGGCA GAATGAGCAC 
TGTCTGACCA ACTTTGACCT TGCGGAATAC AGACAAGTCT TGAGTGATCT GGCTATTCAG 
ATTTACCAGC AGTTGGTCAG AGTGTTGGAA AATATCTTGC AGCCAATGAT TGTTTCAGGA 
ATGCTGGAGC ACGAGACTAT TCAGGGTGTC TCAGGGGTCA AGCCTACCGG TCTTAGGAAG 
AGAACTTCCA GCATAGCAGA TGAAGGGACG TACACATTAG ACTCCATTGT GAGGCAGCTT 
AATACCTTCC ATTCCATAAT GTGTCAGCAT GGGATGGATC CTGAACTCAT CAAGCAGGTG 
GTCAAGCAAA TGTTCTACAT CATCGGTGCT GTCACACTCA ACAACTTGCT CTTGCGAAAG 
GACATGTGCT CTTGGAGCAA AGGCATGCAG ATAAGGTACA ACGTGAGCCA GTTGGAAGAA 
TGGCTGCGTG ACAAGAACCT GATGAATAGC AGCGCTAAAG AAACCCTGGA GCCATTAATT 
CAGGCTGCTC AGTTACTGCA GGTGAAAAAG AAAACAGACG AAGATGCAGA AGCCATCTGC 
TCCATGTGCA ATGCTCTGAC TACTGCCCAG ATTGTCAAGG TGTTGAACCT GTACACTCCA 
GTAAATGAGT TTGAAGAGCG GGTGTTGGTA TCATTTATAC GGAATATACA GATGCGTTTA 
AGGGATAGGA AGGACTCCCC TCAGCTGCTA ATGGATGCTA AGCATATCTT CCCTGTCACC 
TTCCCATTCA ACCCTTCGTC GCTTGCCCTA GAAACAATTC AGATTCCTGG CAGTCTTGGC 
CTTGCCTTCC TCACACGTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755041.1
CDS	354..6017
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDDKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNSLETIERNLPPDYQALNEDGELWLVYDGLKQANRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLFFEELYADEPMKYQSYRISLYRRIIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_031899181.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899181.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGTTTAC ACTGCTAACA GAACTGTTCC AAGAAGAGGA GCAGATCTTG 
AGCCCTACAT CTTCTGCACC ACCCTCAGGC CGTACTCTGC TATCCCGCAC TGGTCTGCGT 
TCCCTCAAGC CCAAGCCTGA ACAGACAAGC AAGGAGCATA AAAAAACTGT AGGACATCAG 
TTCAGAAATT CCCTTCACCT TCTGATGGAG ACTCTCAATG CCACAACTCC ACATTATGTC 
CGTTGCATTA AGCCAAATGA CTTCAAGTAC CCGTTCACAT TTGACTCTAA ACGAGCAGTG 
CAGCAGCTTA GGGCTTGTGG CGTCCTGGAA ACCATCCGAA TCAGTGCTGC TGGATTTCCT 
TCTAGGTGGA CATATCAGGA ATTTTTTAGC AGATATCGTG TTTTAATGAA GCAAAAAGAT 
GTCCTCAGTG ATAGGAAGCA AACCTGCAAA AATGTTCTGG AGAAGCTTAT TTTGGATAAG 
GATAAGTACC AGTTTGGTAA GACAAAGATT TTTTTCCGTG CTGGCCAAGT GGCCTATCTG 
GAGAAGATCC GAGCAGACAA GCTGAGAATG GCCTGTATCA GGATCCAGAA GACAATCCGA 
GGATGGCTTT TGAGGAAGAA ATACTTGCGC ATGAGGAAAG CAGCCATAAC AATCCAGCGC 
TACGTCAGAG GATACCAGGC ACGATGTTAT GCACAGTTTC TGCGTAGAAC ACAGGCCGCG 
ATTATTATCC AGAAATTCCA AAGGATGTAT GTCGTTCGGC AAAAGTACCG TCATATGCAG 
TCCATTACCC TTGCTCTCCA GTCGTATATG CGTGGTTATG CAGCCAGGAA GAGATTTCAG 
GGGATGCTTA GAGCACACAA AGCTACCATC ATCCAGAAGC ATGTGCGCGG CTGGTTAGCC 
CGTGTCACTT ATAAGAGAAA TTTGAGTGCT ATTGTGTACC TGCAGTGTTG TTACCGCAGG 
ATGATGGCCA AAAGGGAGCT GAAGAAGTTA AAGATAGAGG CACGCTCAGT GGAACACTAC 
AAGAAACTCA ACAATGGGAT GGAAAACAAG ATCATGCAGT TGCAGCGTAA AGTTGATGAG 
CAGAATAAAG ACAACAAATC CTTGCTGGAG AGGCTAACAC ACCTGGAGGT CACGTACAAT 
ACTGAAAAAG ATAAGCTGAG AAGCGACGTC GACAGACTGC GCCATTTTGA AGAGGAGGCC 
AAAAATGCAG CAAACAGGAT GGTCAGCTTG CAGGACGAGC TTGCCAGGCT TAGGAAAGAG 
CTGCAGCAAA CACAGACGGA AAAGAACAAG ATTGAGGAAC GGGCAGAGAA GTACCAGACC 
GAGACTGAAA AGCTGGTTGC TGAGCTGAGA GAACAGAATG CTTTGCTGAA AGCTGAGAAA 
GAGGAACTGA ATCTTTTAAT CCAGGAGCAG GCAAAAAAAA TGACAGAGGA TATGGAAAAG 
AAAATTATAG AAGAAACAAA GCAGTTGGAG CTTGAGCTGA ATGATGAACG ACTGAGGTAC 
CAGAATCTCC TAAACGAGTA CAGCCGCCTG GAGGAGCGAT ATGATGACCT TAAAGATGAA 
ATTAATACCA TGAGTCCTCC AAAGCCAGGA CACAAGCGCA CAGACTCTAC TCATAGCAGC 
AATGAATCAG AATACACTTT TAGTTCAGAG ATCACAGAGT CAGAAGATTA CCCATATCGA 
AATGAGGAGC CTGCTGACAG AAAAACTCCG CTGGACATGT CCCTGTTCCT TAAGCTTCAG 
AAACGGGTAA AGGAACTTGA GCAGGAAAAG CAAGTAATGC AAGATGATTT GGATAAGAAG 
GAGGAGCAGA TAATCCGAGC CAAAATAATG GAGGAGGCAA AGACTCCAAC TAGAGGTACA 
GAACTGGAAT ATGAATCACT CAAGCGTCAA GAACTGGAAT CTGAAAATAA AAAACTTAAG 
AATGAGTTAA ATGAGCTGCG AAGGGCAATC ACTGAGAAAG CCTCCCCAGA TGCTACAGGC 
CCTGGTGGAA CTGTCTATAA AGTTCTCTTG GAGCAGATGA CGTCAGTAAG TGAGGAACTT 
GAAGTACGCA AGGAAGAAGT CCTTATACTG AGGTCCCAGT TGGTGAGCCA AATGGAAGCC 
ATTCCACACA AGGATGACAA GGACACAATG ACCGATTCCA TGGTCCTCTC TGAGGATGTA 
CAGAAGATGA AGGATAAAAG GGAAATTGCT CAGGCCTACA CTGGCATGAA GGAGACAAAT 
AGTCTTGAAA CTATTGAAAG AAACCTGCCT CCAGACTACC AAGCCCTAAA TGAGGACGGA 
GAGCTGTGGC TGGTTTATGA TGGGTTAAAA CAGGCCAACA GGGTTTTGGA ATCCCAGCTG 
CAATCACAGA AAAAGGCCTT TGAGAATGAA GTGGAAGCTC TCAGAGGTGA GATCCAGGCT 
CTAAAAGAGG AGAACAACCG GCAGCAGCAA CTTTTAGCCC AGAACCTCCA GTTGCCACCT 
GAAGCACGAA TCGAAGCCAG CTTGCAGCAT GAAATCACCA GGCTAACCAA CGAGAACTTG 
TTTTTTGAGG AGTTATATGC AGATGAACCC ATGAAGTATC AATCGTACCG GATCTCCCTG 
TACAGACGGA TCATTGATCT GATGGAGCAA CTTGAAAAGC AAGATAAGAC TGTCCGCAAA 
CTGAAAAAGC AGTTGAAAGT CTTTGCCAAG AAAATTGGAG AGCTGGAAGT TGGTCAAATG 
GAAAATGTTT CTCCTGGTCA GATAGTGGAT GAACCCATTC GTCCTGTTAA TATTCCGCGC 
AAAGAGAAGG ACTTCCAAGG AATGCTGGAA TATAAGAAAG AGGACGAACT AAAGCTTGTC 
AAAAACCTCA TTTTAGAACT GAAGCCTCGT GGTGTCGCGG TCAACCTCAT TCCAGGACTG 
CCAGCATATA TCTTGTTCAT GTGTTTGCGT CATGCTGATT ACCTCAATGA TGATCAGAAA 
GTTCGCTCCT TGCTTACATC AACTATTAAT GGTATCAAGA AAATCTTAAA GAAAAGAGGA 
GATGATTTTG AAACGGTGTC CTTCTGGCTG TCCAATTCGT GTCGGTTCCT CCATTGCTTG 
AAGCAGTACA GTGGAGAAGA GGGATTTATG AAGCACAACA CCACACGGCA GAATGAGCAC 
TGTCTGACCA ACTTTGACCT TGCGGAATAC AGACAAGTCT TGAGTGATCT GGCTATTCAG 
ATTTACCAGC AGTTGGTCAG AGTGTTGGAA AATATCTTGC AGCCAATGAT TGTTTCAGGA 
ATGCTGGAGC ACGAGACTAT TCAGGGTGTC TCAGGGGTCA AGCCTACCGG TCTTAGGAAG 
AGAACTTCCA GCATAGCAGA TGAAGGGACG TACACATTAG ACTCCATTGT GAGGCAGCTT 
AATACCTTCC ATTCCATAAT GTGTCAGCAT GGGATGGATC CTGAACTCAT CAAGCAGGTG 
GTCAAGCAAA TGTTCTACAT CATCGGTGCT GTCACACTCA ACAACTTGCT CTTGCGAAAG 
GACATGTGCT CTTGGAGCAA AGGCATGCAG ATAAGGTACA ACGTGAGCCA GTTGGAAGAA 
TGGCTGCGTG ACAAGAACCT GATGAATAGC AGCGCTAAAG AAACCCTGGA GCCATTAATT 
CAGGCTGCTC AGTTACTGCA GGTGAAAAAG AAAACAGACG AAGATGCAGA AGCCATCTGC 
TCCATGTGCA ATGCTCTGAC TACTGCCCAG ATTGTCAAGG TGTTGAACCT GTACACTCCA 
GTAAATGAGT TTGAAGAGCG GGTGTTGGTA TCATTTATAC GGAATATACA GATGCGTTTA 
AGGGATAGGA AGGACTCCCC TCAGCTGCTA ATGGATGCTA AGCATATCTT CCCTGTCACC 
TTCCCATTCA ACCCTTCGTC GCTTGCCCTA GAAACAATTC AGATTCCTGG CAGTCTTGGC 
CTTGCCTTCC TCACACGTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_031899182.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755042.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa55091
Clone ID Related Accession (Same CDS sequence)	XM_031899182.1
Accession Version	XM_031899182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACA GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAAA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ATGACAAGGA CACAATGACC GATTCCATGG TCCTCTCTGA GGATGTACAG 
AAGATGAAGG ATAAAAGGGA AATTGCTCAG GCCTACACTG GCATGAAGGA GACAAATAGT 
CTTGAAACTA TTGAAAGAAA CCTGCCTCCA GACTACCAAG CCCTAAATGA GGACGGAGAG 
CTGTGGCTGG TTTATGATGG GTTAAAACAG GCCAACAGGG TTTTGGAATC CCAGCTGCAA 
TCACAGAAAA AGGCCTTTGA GAATGAAGTG GAAGCTCTCA GAGGTGAGAT CCAGGCTCTA 
AAAGAGGAGA ACAACCGGCA GCAGCAACTT TTAGCCCAGA ACCTCCAGTT GCCACCTGAA 
GCACGAATCG AAGCCAGCTT GCAGCATGAA ATCACCAGGC TAACCAACGA GAACTTGTTT 
TTTGAGGAGT TATATGCAGA TGAACCCATG AAGTATCAAT CGTACCGGAT CTCCCTGTAC 
AGACGGATCA TTGATCTGAT GGAGCAACTT GAAAAGCAAG ATAAGACTGT CCGCAAACTG 
AAAAAGCAGT TGAAAGTCTT TGCCAAGAAA ATTGGAGAGC TGGAAGTTGG TCAAATGGAA 
AATGTTTCTC CTGGTCAGAT AGTGGATGAA CCCATTCGTC CTGTTAATAT TCCGCGCAAA 
GAGAAGGACT TCCAAGGAAT GCTGGAATAT AAGAAAGAGG ACGAACTAAA GCTTGTCAAA 
AACCTCATTT TAGAACTGAA GCCTCGTGGT GTCGCGGTCA ACCTCATTCC AGGACTGCCA 
GCATATATCT TGTTCATGTG TTTGCGTCAT GCTGATTACC TCAATGATGA TCAGAAAGTT 
CGCTCCTTGC TTACATCAAC TATTAATGGT ATCAAGAAAA TCTTAAAGAA AAGAGGAGAT 
GATTTTGAAA CGGTGTCCTT CTGGCTGTCC AATTCGTGTC GGTTCCTCCA TTGCTTGAAG 
CAGTACAGTG GAGAAGAGGG ATTTATGAAG CACAACACCA CACGGCAGAA TGAGCACTGT 
CTGACCAACT TTGACCTTGC GGAATACAGA CAAGTCTTGA GTGATCTGGC TATTCAGATT 
TACCAGCAGT TGGTCAGAGT GTTGGAAAAT ATCTTGCAGC CAATGATTGT TTCAGGAATG 
CTGGAGCACG AGACTATTCA GGGTGTCTCA GGGGTCAAGC CTACCGGTCT TAGGAAGAGA 
ACTTCCAGCA TAGCAGATGA AGGGACGTAC ACATTAGACT CCATTGTGAG GCAGCTTAAT 
ACCTTCCATT CCATAATGTG TCAGCATGGG ATGGATCCTG AACTCATCAA GCAGGTGGTC 
AAGCAAATGT TCTACATCAT CGGTGCTGTC ACACTCAACA ACTTGCTCTT GCGAAAGGAC 
ATGTGCTCTT GGAGCAAAGG CATGCAGATA AGGTACAACG TGAGCCAGTT GGAAGAATGG 
CTGCGTGACA AGAACCTGAT GAATAGCAGC GCTAAAGAAA CCCTGGAGCC ATTAATTCAG 
GCTGCTCAGT TACTGCAGGT GAAAAAGAAA ACAGACGAAG ATGCAGAAGC CATCTGCTCC 
ATGTGCAATG CTCTGACTAC TGCCCAGATT GTCAAGGTGT TGAACCTGTA CACTCCAGTA 
AATGAGTTTG AAGAGCGGGT GTTGGTATCA TTTATACGGA ATATACAGAT GCGTTTAAGG 
GATAGGAAGG ACTCCCCTCA GCTGCTAATG GATGCTAAGC ATATCTTCCC TGTCACCTTC 
CCATTCAACC CTTCGTCGCT TGCCCTAGAA ACAATTCAGA TTCCTGGCAG TCTTGGCCTT 
GCCTTCCTCA CACGTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755042.1
CDS	354..6014
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDDKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNSLETIERNLPPDYQALNEDGELWLVYDGLKQANRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLFFEELYADEPMKYQSYRISLYRRIIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_031899182.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899182.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACA GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAAA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ATGACAAGGA CACAATGACC GATTCCATGG TCCTCTCTGA GGATGTACAG 
AAGATGAAGG ATAAAAGGGA AATTGCTCAG GCCTACACTG GCATGAAGGA GACAAATAGT 
CTTGAAACTA TTGAAAGAAA CCTGCCTCCA GACTACCAAG CCCTAAATGA GGACGGAGAG 
CTGTGGCTGG TTTATGATGG GTTAAAACAG GCCAACAGGG TTTTGGAATC CCAGCTGCAA 
TCACAGAAAA AGGCCTTTGA GAATGAAGTG GAAGCTCTCA GAGGTGAGAT CCAGGCTCTA 
AAAGAGGAGA ACAACCGGCA GCAGCAACTT TTAGCCCAGA ACCTCCAGTT GCCACCTGAA 
GCACGAATCG AAGCCAGCTT GCAGCATGAA ATCACCAGGC TAACCAACGA GAACTTGTTT 
TTTGAGGAGT TATATGCAGA TGAACCCATG AAGTATCAAT CGTACCGGAT CTCCCTGTAC 
AGACGGATCA TTGATCTGAT GGAGCAACTT GAAAAGCAAG ATAAGACTGT CCGCAAACTG 
AAAAAGCAGT TGAAAGTCTT TGCCAAGAAA ATTGGAGAGC TGGAAGTTGG TCAAATGGAA 
AATGTTTCTC CTGGTCAGAT AGTGGATGAA CCCATTCGTC CTGTTAATAT TCCGCGCAAA 
GAGAAGGACT TCCAAGGAAT GCTGGAATAT AAGAAAGAGG ACGAACTAAA GCTTGTCAAA 
AACCTCATTT TAGAACTGAA GCCTCGTGGT GTCGCGGTCA ACCTCATTCC AGGACTGCCA 
GCATATATCT TGTTCATGTG TTTGCGTCAT GCTGATTACC TCAATGATGA TCAGAAAGTT 
CGCTCCTTGC TTACATCAAC TATTAATGGT ATCAAGAAAA TCTTAAAGAA AAGAGGAGAT 
GATTTTGAAA CGGTGTCCTT CTGGCTGTCC AATTCGTGTC GGTTCCTCCA TTGCTTGAAG 
CAGTACAGTG GAGAAGAGGG ATTTATGAAG CACAACACCA CACGGCAGAA TGAGCACTGT 
CTGACCAACT TTGACCTTGC GGAATACAGA CAAGTCTTGA GTGATCTGGC TATTCAGATT 
TACCAGCAGT TGGTCAGAGT GTTGGAAAAT ATCTTGCAGC CAATGATTGT TTCAGGAATG 
CTGGAGCACG AGACTATTCA GGGTGTCTCA GGGGTCAAGC CTACCGGTCT TAGGAAGAGA 
ACTTCCAGCA TAGCAGATGA AGGGACGTAC ACATTAGACT CCATTGTGAG GCAGCTTAAT 
ACCTTCCATT CCATAATGTG TCAGCATGGG ATGGATCCTG AACTCATCAA GCAGGTGGTC 
AAGCAAATGT TCTACATCAT CGGTGCTGTC ACACTCAACA ACTTGCTCTT GCGAAAGGAC 
ATGTGCTCTT GGAGCAAAGG CATGCAGATA AGGTACAACG TGAGCCAGTT GGAAGAATGG 
CTGCGTGACA AGAACCTGAT GAATAGCAGC GCTAAAGAAA CCCTGGAGCC ATTAATTCAG 
GCTGCTCAGT TACTGCAGGT GAAAAAGAAA ACAGACGAAG ATGCAGAAGC CATCTGCTCC 
ATGTGCAATG CTCTGACTAC TGCCCAGATT GTCAAGGTGT TGAACCTGTA CACTCCAGTA 
AATGAGTTTG AAGAGCGGGT GTTGGTATCA TTTATACGGA ATATACAGAT GCGTTTAAGG 
GATAGGAAGG ACTCCCCTCA GCTGCTAATG GATGCTAAGC ATATCTTCCC TGTCACCTTC 
CCATTCAACC CTTCGTCGCT TGCCCTAGAA ACAATTCAGA TTCCTGGCAG TCTTGGCCTT 
GCCTTCCTCA CACGTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_031899183.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755043.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa55092
Clone ID Related Accession (Same CDS sequence)	XM_031899183.1
Accession Version	XM_031899183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGACAC AATGACCGAT TCCATGGTCC TCTCTGAGGA TGTACAGAAG 
ATGAAGGATA AAAGGGAAAT TGCTCAGGCC TACACTGGCA TGAAGGAGAC AAATAGTCTT 
GAAACTATTG AAAGAAACCT GCCTCCAGAC TACCAAGCCC TAAATGAGGA CGGAGAGCTG 
TGGCTGGTTT ATGATGGGTT AAAACAGGCC AACAGGGTTT TGGAATCCCA GCTGCAATCA 
CAGAAAAAGG CCTTTGAGAA TGAAGTGGAA GCTCTCAGAG GTGAGATCCA GGCTCTAAAA 
GAGGAGAACA ACCGGCAGCA GCAACTTTTA GCCCAGAACC TCCAGTTGCC ACCTGAAGCA 
CGAATCGAAG CCAGCTTGCA GCATGAAATC ACCAGGCTAA CCAACGAGAA CTTGTTTTTT 
GAGGAGTTAT ATGCAGATGA ACCCATGAAG TATCAATCGT ACCGGATCTC CCTGTACAGA 
CGGATCATTG ATCTGATGGA GCAACTTGAA AAGCAAGATA AGACTGTCCG CAAACTGAAA 
AAGCAGTTGA AAGTCTTTGC CAAGAAAATT GGAGAGCTGG AAGTTGGTCA AATGGAAAAT 
GTTTCTCCTG GTCAGATAGT GGATGAACCC ATTCGTCCTG TTAATATTCC GCGCAAAGAG 
AAGGACTTCC AAGGAATGCT GGAATATAAG AAAGAGGACG AACTAAAGCT TGTCAAAAAC 
CTCATTTTAG AACTGAAGCC TCGTGGTGTC GCGGTCAACC TCATTCCAGG ACTGCCAGCA 
TATATCTTGT TCATGTGTTT GCGTCATGCT GATTACCTCA ATGATGATCA GAAAGTTCGC 
TCCTTGCTTA CATCAACTAT TAATGGTATC AAGAAAATCT TAAAGAAAAG AGGAGATGAT 
TTTGAAACGG TGTCCTTCTG GCTGTCCAAT TCGTGTCGGT TCCTCCATTG CTTGAAGCAG 
TACAGTGGAG AAGAGGGATT TATGAAGCAC AACACCACAC GGCAGAATGA GCACTGTCTG 
ACCAACTTTG ACCTTGCGGA ATACAGACAA GTCTTGAGTG ATCTGGCTAT TCAGATTTAC 
CAGCAGTTGG TCAGAGTGTT GGAAAATATC TTGCAGCCAA TGATTGTTTC AGGAATGCTG 
GAGCACGAGA CTATTCAGGG TGTCTCAGGG GTCAAGCCTA CCGGTCTTAG GAAGAGAACT 
TCCAGCATAG CAGATGAAGG GACGTACACA TTAGACTCCA TTGTGAGGCA GCTTAATACC 
TTCCATTCCA TAATGTGTCA GCATGGGATG GATCCTGAAC TCATCAAGCA GGTGGTCAAG 
CAAATGTTCT ACATCATCGG TGCTGTCACA CTCAACAACT TGCTCTTGCG AAAGGACATG 
TGCTCTTGGA GCAAAGGCAT GCAGATAAGG TACAACGTGA GCCAGTTGGA AGAATGGCTG 
CGTGACAAGA ACCTGATGAA TAGCAGCGCT AAAGAAACCC TGGAGCCATT AATTCAGGCT 
GCTCAGTTAC TGCAGGTGAA AAAGAAAACA GACGAAGATG CAGAAGCCAT CTGCTCCATG 
TGCAATGCTC TGACTACTGC CCAGATTGTC AAGGTGTTGA ACCTGTACAC TCCAGTAAAT 
GAGTTTGAAG AGCGGGTGTT GGTATCATTT ATACGGAATA TACAGATGCG TTTAAGGGAT 
AGGAAGGACT CCCCTCAGCT GCTAATGGAT GCTAAGCATA TCTTCCCTGT CACCTTCCCA 
TTCAACCCTT CGTCGCTTGC CCTAGAAACA ATTCAGATTC CTGGCAGTCT TGGCCTTGCC 
TTCCTCACAC GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755043.1
CDS	354..6011
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKKKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNSLETIERNLPPDYQALNEDGELWLVYDGLKQANRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLFFEELYADEPMKYQSYRISLYRRIIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_031899183.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899183.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGACAC AATGACCGAT TCCATGGTCC TCTCTGAGGA TGTACAGAAG 
ATGAAGGATA AAAGGGAAAT TGCTCAGGCC TACACTGGCA TGAAGGAGAC AAATAGTCTT 
GAAACTATTG AAAGAAACCT GCCTCCAGAC TACCAAGCCC TAAATGAGGA CGGAGAGCTG 
TGGCTGGTTT ATGATGGGTT AAAACAGGCC AACAGGGTTT TGGAATCCCA GCTGCAATCA 
CAGAAAAAGG CCTTTGAGAA TGAAGTGGAA GCTCTCAGAG GTGAGATCCA GGCTCTAAAA 
GAGGAGAACA ACCGGCAGCA GCAACTTTTA GCCCAGAACC TCCAGTTGCC ACCTGAAGCA 
CGAATCGAAG CCAGCTTGCA GCATGAAATC ACCAGGCTAA CCAACGAGAA CTTGTTTTTT 
GAGGAGTTAT ATGCAGATGA ACCCATGAAG TATCAATCGT ACCGGATCTC CCTGTACAGA 
CGGATCATTG ATCTGATGGA GCAACTTGAA AAGCAAGATA AGACTGTCCG CAAACTGAAA 
AAGCAGTTGA AAGTCTTTGC CAAGAAAATT GGAGAGCTGG AAGTTGGTCA AATGGAAAAT 
GTTTCTCCTG GTCAGATAGT GGATGAACCC ATTCGTCCTG TTAATATTCC GCGCAAAGAG 
AAGGACTTCC AAGGAATGCT GGAATATAAG AAAGAGGACG AACTAAAGCT TGTCAAAAAC 
CTCATTTTAG AACTGAAGCC TCGTGGTGTC GCGGTCAACC TCATTCCAGG ACTGCCAGCA 
TATATCTTGT TCATGTGTTT GCGTCATGCT GATTACCTCA ATGATGATCA GAAAGTTCGC 
TCCTTGCTTA CATCAACTAT TAATGGTATC AAGAAAATCT TAAAGAAAAG AGGAGATGAT 
TTTGAAACGG TGTCCTTCTG GCTGTCCAAT TCGTGTCGGT TCCTCCATTG CTTGAAGCAG 
TACAGTGGAG AAGAGGGATT TATGAAGCAC AACACCACAC GGCAGAATGA GCACTGTCTG 
ACCAACTTTG ACCTTGCGGA ATACAGACAA GTCTTGAGTG ATCTGGCTAT TCAGATTTAC 
CAGCAGTTGG TCAGAGTGTT GGAAAATATC TTGCAGCCAA TGATTGTTTC AGGAATGCTG 
GAGCACGAGA CTATTCAGGG TGTCTCAGGG GTCAAGCCTA CCGGTCTTAG GAAGAGAACT 
TCCAGCATAG CAGATGAAGG GACGTACACA TTAGACTCCA TTGTGAGGCA GCTTAATACC 
TTCCATTCCA TAATGTGTCA GCATGGGATG GATCCTGAAC TCATCAAGCA GGTGGTCAAG 
CAAATGTTCT ACATCATCGG TGCTGTCACA CTCAACAACT TGCTCTTGCG AAAGGACATG 
TGCTCTTGGA GCAAAGGCAT GCAGATAAGG TACAACGTGA GCCAGTTGGA AGAATGGCTG 
CGTGACAAGA ACCTGATGAA TAGCAGCGCT AAAGAAACCC TGGAGCCATT AATTCAGGCT 
GCTCAGTTAC TGCAGGTGAA AAAGAAAACA GACGAAGATG CAGAAGCCAT CTGCTCCATG 
TGCAATGCTC TGACTACTGC CCAGATTGTC AAGGTGTTGA ACCTGTACAC TCCAGTAAAT 
GAGTTTGAAG AGCGGGTGTT GGTATCATTT ATACGGAATA TACAGATGCG TTTAAGGGAT 
AGGAAGGACT CCCCTCAGCT GCTAATGGAT GCTAAGCATA TCTTCCCTGT CACCTTCCCA 
TTCAACCCTT CGTCGCTTGC CCTAGAAACA ATTCAGATTC CTGGCAGTCT TGGCCTTGCC 
TTCCTCACAC GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_031899184.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755044.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X5, mRNA.

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CloneID	OXa55093
Clone ID Related Accession (Same CDS sequence)	XM_031899184.1
Accession Version	XM_031899184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACA GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAAA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ACACAATGAC CGATTCCATG GTCCTCTCTG AGGATGTACA GAAGATGAAG 
GATAAAAGGG AAATTGCTCA GGCCTACACT GGCATGAAGG AGACAAATAG TCTTGAAACT 
ATTGAAAGAA ACCTGCCTCC AGACTACCAA GCCCTAAATG AGGACGGAGA GCTGTGGCTG 
GTTTATGATG GGTTAAAACA GGCCAACAGG GTTTTGGAAT CCCAGCTGCA ATCACAGAAA 
AAGGCCTTTG AGAATGAAGT GGAAGCTCTC AGAGGTGAGA TCCAGGCTCT AAAAGAGGAG 
AACAACCGGC AGCAGCAACT TTTAGCCCAG AACCTCCAGT TGCCACCTGA AGCACGAATC 
GAAGCCAGCT TGCAGCATGA AATCACCAGG CTAACCAACG AGAACTTGTT TTTTGAGGAG 
TTATATGCAG ATGAACCCAT GAAGTATCAA TCGTACCGGA TCTCCCTGTA CAGACGGATC 
ATTGATCTGA TGGAGCAACT TGAAAAGCAA GATAAGACTG TCCGCAAACT GAAAAAGCAG 
TTGAAAGTCT TTGCCAAGAA AATTGGAGAG CTGGAAGTTG GTCAAATGGA AAATGTTTCT 
CCTGGTCAGA TAGTGGATGA ACCCATTCGT CCTGTTAATA TTCCGCGCAA AGAGAAGGAC 
TTCCAAGGAA TGCTGGAATA TAAGAAAGAG GACGAACTAA AGCTTGTCAA AAACCTCATT 
TTAGAACTGA AGCCTCGTGG TGTCGCGGTC AACCTCATTC CAGGACTGCC AGCATATATC 
TTGTTCATGT GTTTGCGTCA TGCTGATTAC CTCAATGATG ATCAGAAAGT TCGCTCCTTG 
CTTACATCAA CTATTAATGG TATCAAGAAA ATCTTAAAGA AAAGAGGAGA TGATTTTGAA 
ACGGTGTCCT TCTGGCTGTC CAATTCGTGT CGGTTCCTCC ATTGCTTGAA GCAGTACAGT 
GGAGAAGAGG GATTTATGAA GCACAACACC ACACGGCAGA ATGAGCACTG TCTGACCAAC 
TTTGACCTTG CGGAATACAG ACAAGTCTTG AGTGATCTGG CTATTCAGAT TTACCAGCAG 
TTGGTCAGAG TGTTGGAAAA TATCTTGCAG CCAATGATTG TTTCAGGAAT GCTGGAGCAC 
GAGACTATTC AGGGTGTCTC AGGGGTCAAG CCTACCGGTC TTAGGAAGAG AACTTCCAGC 
ATAGCAGATG AAGGGACGTA CACATTAGAC TCCATTGTGA GGCAGCTTAA TACCTTCCAT 
TCCATAATGT GTCAGCATGG GATGGATCCT GAACTCATCA AGCAGGTGGT CAAGCAAATG 
TTCTACATCA TCGGTGCTGT CACACTCAAC AACTTGCTCT TGCGAAAGGA CATGTGCTCT 
TGGAGCAAAG GCATGCAGAT AAGGTACAAC GTGAGCCAGT TGGAAGAATG GCTGCGTGAC 
AAGAACCTGA TGAATAGCAG CGCTAAAGAA ACCCTGGAGC CATTAATTCA GGCTGCTCAG 
TTACTGCAGG TGAAAAAGAA AACAGACGAA GATGCAGAAG CCATCTGCTC CATGTGCAAT 
GCTCTGACTA CTGCCCAGAT TGTCAAGGTG TTGAACCTGT ACACTCCAGT AAATGAGTTT 
GAAGAGCGGG TGTTGGTATC ATTTATACGG AATATACAGA TGCGTTTAAG GGATAGGAAG 
GACTCCCCTC AGCTGCTAAT GGATGCTAAG CATATCTTCC CTGTCACCTT CCCATTCAAC 
CCTTCGTCGC TTGCCCTAGA AACAATTCAG ATTCCTGGCA GTCTTGGCCT TGCCTTCCTC 
ACACGTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755044.1
CDS	354..6005
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNSLETIERNLPPDYQALNEDGELWLVYDGLKQANRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLFFEELYADEPMKYQSYRISLYRRIIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_031899184.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899184.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACA GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAAA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ACACAATGAC CGATTCCATG GTCCTCTCTG AGGATGTACA GAAGATGAAG 
GATAAAAGGG AAATTGCTCA GGCCTACACT GGCATGAAGG AGACAAATAG TCTTGAAACT 
ATTGAAAGAA ACCTGCCTCC AGACTACCAA GCCCTAAATG AGGACGGAGA GCTGTGGCTG 
GTTTATGATG GGTTAAAACA GGCCAACAGG GTTTTGGAAT CCCAGCTGCA ATCACAGAAA 
AAGGCCTTTG AGAATGAAGT GGAAGCTCTC AGAGGTGAGA TCCAGGCTCT AAAAGAGGAG 
AACAACCGGC AGCAGCAACT TTTAGCCCAG AACCTCCAGT TGCCACCTGA AGCACGAATC 
GAAGCCAGCT TGCAGCATGA AATCACCAGG CTAACCAACG AGAACTTGTT TTTTGAGGAG 
TTATATGCAG ATGAACCCAT GAAGTATCAA TCGTACCGGA TCTCCCTGTA CAGACGGATC 
ATTGATCTGA TGGAGCAACT TGAAAAGCAA GATAAGACTG TCCGCAAACT GAAAAAGCAG 
TTGAAAGTCT TTGCCAAGAA AATTGGAGAG CTGGAAGTTG GTCAAATGGA AAATGTTTCT 
CCTGGTCAGA TAGTGGATGA ACCCATTCGT CCTGTTAATA TTCCGCGCAA AGAGAAGGAC 
TTCCAAGGAA TGCTGGAATA TAAGAAAGAG GACGAACTAA AGCTTGTCAA AAACCTCATT 
TTAGAACTGA AGCCTCGTGG TGTCGCGGTC AACCTCATTC CAGGACTGCC AGCATATATC 
TTGTTCATGT GTTTGCGTCA TGCTGATTAC CTCAATGATG ATCAGAAAGT TCGCTCCTTG 
CTTACATCAA CTATTAATGG TATCAAGAAA ATCTTAAAGA AAAGAGGAGA TGATTTTGAA 
ACGGTGTCCT TCTGGCTGTC CAATTCGTGT CGGTTCCTCC ATTGCTTGAA GCAGTACAGT 
GGAGAAGAGG GATTTATGAA GCACAACACC ACACGGCAGA ATGAGCACTG TCTGACCAAC 
TTTGACCTTG CGGAATACAG ACAAGTCTTG AGTGATCTGG CTATTCAGAT TTACCAGCAG 
TTGGTCAGAG TGTTGGAAAA TATCTTGCAG CCAATGATTG TTTCAGGAAT GCTGGAGCAC 
GAGACTATTC AGGGTGTCTC AGGGGTCAAG CCTACCGGTC TTAGGAAGAG AACTTCCAGC 
ATAGCAGATG AAGGGACGTA CACATTAGAC TCCATTGTGA GGCAGCTTAA TACCTTCCAT 
TCCATAATGT GTCAGCATGG GATGGATCCT GAACTCATCA AGCAGGTGGT CAAGCAAATG 
TTCTACATCA TCGGTGCTGT CACACTCAAC AACTTGCTCT TGCGAAAGGA CATGTGCTCT 
TGGAGCAAAG GCATGCAGAT AAGGTACAAC GTGAGCCAGT TGGAAGAATG GCTGCGTGAC 
AAGAACCTGA TGAATAGCAG CGCTAAAGAA ACCCTGGAGC CATTAATTCA GGCTGCTCAG 
TTACTGCAGG TGAAAAAGAA AACAGACGAA GATGCAGAAG CCATCTGCTC CATGTGCAAT 
GCTCTGACTA CTGCCCAGAT TGTCAAGGTG TTGAACCTGT ACACTCCAGT AAATGAGTTT 
GAAGAGCGGG TGTTGGTATC ATTTATACGG AATATACAGA TGCGTTTAAG GGATAGGAAG 
GACTCCCCTC AGCTGCTAAT GGATGCTAAG CATATCTTCC CTGTCACCTT CCCATTCAAC 
CCTTCGTCGC TTGCCCTAGA AACAATTCAG ATTCCTGGCA GTCTTGGCCT TGCCTTCCTC 
ACACGTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_031899185.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755045.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X6, mRNA.

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CloneID	OXa55094
Clone ID Related Accession (Same CDS sequence)	XM_031899185.1
Accession Version	XM_031899185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGATGA CAAGGACACA ATGACCGATT CCATGGTCCT CTCTGAGGAT 
GTACAGAAGA TGAAGGATAA AAGGGAAATT GCTCAGGCCT ACACTGGCAT GAAGGAGACA 
AATAGTCTTG AAACTATTGA AAGAAACCTG CCTCCAGACT ACCAAGCCCT AAATGAGGAC 
GGAGAGCTGT GGCTGGTTTA TGATGGGTTA AAACAGGCCA ACAGGGTTTT GGAATCCCAG 
CTGCAATCAC AGAAAAAGGC CTTTGAGAAT GAAGTGGAAG CTCTCAGAGG TGAGATCCAG 
GCTCTAAAAG AGGAGAACAA CCGGCAGCAG CAACTTTTAG CCCAGAACCT CCAGTTGCCA 
CCTGAAGCAC GAATCGAAGC CAGCTTGCAG CATGAAATCA CCAGGCTAAC CAACGAGAAC 
TTGGATCTGA TGGAGCAACT TGAAAAGCAA GATAAGACTG TCCGCAAACT GAAAAAGCAG 
TTGAAAGTCT TTGCCAAGAA AATTGGAGAG CTGGAAGTTG GTCAAATGGA AAATGTTTCT 
CCTGGTCAGA TAGTGGATGA ACCCATTCGT CCTGTTAATA TTCCGCGCAA AGAGAAGGAC 
TTCCAAGGAA TGCTGGAATA TAAGAAAGAG GACGAACTAA AGCTTGTCAA AAACCTCATT 
TTAGAACTGA AGCCTCGTGG TGTCGCGGTC AACCTCATTC CAGGACTGCC AGCATATATC 
TTGTTCATGT GTTTGCGTCA TGCTGATTAC CTCAATGATG ATCAGAAAGT TCGCTCCTTG 
CTTACATCAA CTATTAATGG TATCAAGAAA ATCTTAAAGA AAAGAGGAGA TGATTTTGAA 
ACGGTGTCCT TCTGGCTGTC CAATTCGTGT CGGTTCCTCC ATTGCTTGAA GCAGTACAGT 
GGAGAAGAGG GATTTATGAA GCACAACACC ACACGGCAGA ATGAGCACTG TCTGACCAAC 
TTTGACCTTG CGGAATACAG ACAAGTCTTG AGTGATCTGG CTATTCAGAT TTACCAGCAG 
TTGGTCAGAG TGTTGGAAAA TATCTTGCAG CCAATGATTG TTTCAGGAAT GCTGGAGCAC 
GAGACTATTC AGGGTGTCTC AGGGGTCAAG CCTACCGGTC TTAGGAAGAG AACTTCCAGC 
ATAGCAGATG AAGGGACGTA CACATTAGAC TCCATTGTGA GGCAGCTTAA TACCTTCCAT 
TCCATAATGT GTCAGCATGG GATGGATCCT GAACTCATCA AGCAGGTGGT CAAGCAAATG 
TTCTACATCA TCGGTGCTGT CACACTCAAC AACTTGCTCT TGCGAAAGGA CATGTGCTCT 
TGGAGCAAAG GCATGCAGAT AAGGTACAAC GTGAGCCAGT TGGAAGAATG GCTGCGTGAC 
AAGAACCTGA TGAATAGCAG CGCTAAAGAA ACCCTGGAGC CATTAATTCA GGCTGCTCAG 
TTACTGCAGG TGAAAAAGAA AACAGACGAA GATGCAGAAG CCATCTGCTC CATGTGCAAT 
GCTCTGACTA CTGCCCAGAT TGTCAAGGTG TTGAACCTGT ACACTCCAGT AAATGAGTTT 
GAAGAGCGGG TGTTGGTATC ATTTATACGG AATATACAGA TGCGTTTAAG GGATAGGAAG 
GACTCCCCTC AGCTGCTAAT GGATGCTAAG CATATCTTCC CTGTCACCTT CCCATTCAAC 
CCTTCGTCGC TTGCCCTAGA AACAATTCAG ATTCCTGGCA GTCTTGGCCT TGCCTTCCTC 
ACACGTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755045.1
CDS	353..5944
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKKKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDDKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNSLETIERNLPPDYQALNEDGELWLVYDGLKQANRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_031899185.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899185.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGATGA CAAGGACACA ATGACCGATT CCATGGTCCT CTCTGAGGAT 
GTACAGAAGA TGAAGGATAA AAGGGAAATT GCTCAGGCCT ACACTGGCAT GAAGGAGACA 
AATAGTCTTG AAACTATTGA AAGAAACCTG CCTCCAGACT ACCAAGCCCT AAATGAGGAC 
GGAGAGCTGT GGCTGGTTTA TGATGGGTTA AAACAGGCCA ACAGGGTTTT GGAATCCCAG 
CTGCAATCAC AGAAAAAGGC CTTTGAGAAT GAAGTGGAAG CTCTCAGAGG TGAGATCCAG 
GCTCTAAAAG AGGAGAACAA CCGGCAGCAG CAACTTTTAG CCCAGAACCT CCAGTTGCCA 
CCTGAAGCAC GAATCGAAGC CAGCTTGCAG CATGAAATCA CCAGGCTAAC CAACGAGAAC 
TTGGATCTGA TGGAGCAACT TGAAAAGCAA GATAAGACTG TCCGCAAACT GAAAAAGCAG 
TTGAAAGTCT TTGCCAAGAA AATTGGAGAG CTGGAAGTTG GTCAAATGGA AAATGTTTCT 
CCTGGTCAGA TAGTGGATGA ACCCATTCGT CCTGTTAATA TTCCGCGCAA AGAGAAGGAC 
TTCCAAGGAA TGCTGGAATA TAAGAAAGAG GACGAACTAA AGCTTGTCAA AAACCTCATT 
TTAGAACTGA AGCCTCGTGG TGTCGCGGTC AACCTCATTC CAGGACTGCC AGCATATATC 
TTGTTCATGT GTTTGCGTCA TGCTGATTAC CTCAATGATG ATCAGAAAGT TCGCTCCTTG 
CTTACATCAA CTATTAATGG TATCAAGAAA ATCTTAAAGA AAAGAGGAGA TGATTTTGAA 
ACGGTGTCCT TCTGGCTGTC CAATTCGTGT CGGTTCCTCC ATTGCTTGAA GCAGTACAGT 
GGAGAAGAGG GATTTATGAA GCACAACACC ACACGGCAGA ATGAGCACTG TCTGACCAAC 
TTTGACCTTG CGGAATACAG ACAAGTCTTG AGTGATCTGG CTATTCAGAT TTACCAGCAG 
TTGGTCAGAG TGTTGGAAAA TATCTTGCAG CCAATGATTG TTTCAGGAAT GCTGGAGCAC 
GAGACTATTC AGGGTGTCTC AGGGGTCAAG CCTACCGGTC TTAGGAAGAG AACTTCCAGC 
ATAGCAGATG AAGGGACGTA CACATTAGAC TCCATTGTGA GGCAGCTTAA TACCTTCCAT 
TCCATAATGT GTCAGCATGG GATGGATCCT GAACTCATCA AGCAGGTGGT CAAGCAAATG 
TTCTACATCA TCGGTGCTGT CACACTCAAC AACTTGCTCT TGCGAAAGGA CATGTGCTCT 
TGGAGCAAAG GCATGCAGAT AAGGTACAAC GTGAGCCAGT TGGAAGAATG GCTGCGTGAC 
AAGAACCTGA TGAATAGCAG CGCTAAAGAA ACCCTGGAGC CATTAATTCA GGCTGCTCAG 
TTACTGCAGG TGAAAAAGAA AACAGACGAA GATGCAGAAG CCATCTGCTC CATGTGCAAT 
GCTCTGACTA CTGCCCAGAT TGTCAAGGTG TTGAACCTGT ACACTCCAGT AAATGAGTTT 
GAAGAGCGGG TGTTGGTATC ATTTATACGG AATATACAGA TGCGTTTAAG GGATAGGAAG 
GACTCCCCTC AGCTGCTAAT GGATGCTAAG CATATCTTCC CTGTCACCTT CCCATTCAAC 
CCTTCGTCGC TTGCCCTAGA AACAATTCAG ATTCCTGGCA GTCTTGGCCT TGCCTTCCTC 
ACACGTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_031899186.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755046.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X7, mRNA.

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CloneID	OXa55095
Clone ID Related Accession (Same CDS sequence)	XM_031899186.1
Accession Version	XM_031899186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGATGA CAAGGACACA ATGACCGATT CCATGGTCCT CTCTGAGGAT 
GTACAGAAGA TGAAGGATAA AAGGGAAATT GCTCAGGCCT ACACTGGCAT GAAGGAGACA 
AATAGGGTTT TGGAATCCCA GCTGCAATCA CAGAAAAAGG CCTTTGAGAA TGAAGTGGAA 
GCTCTCAGAG GTGAGATCCA GGCTCTAAAA GAGGAGAACA ACCGGCAGCA GCAACTTTTA 
GCCCAGAACC TCCAGTTGCC ACCTGAAGCA CGAATCGAAG CCAGCTTGCA GCATGAAATC 
ACCAGGCTAA CCAACGAGAA CTTGTTTTTT GAGGAGTTAT ATGCAGATGA ACCCATGAAG 
TATCAATCGT ACCGGATCTC CCTGTACAGA CGGATCATTG ATCTGATGGA GCAACTTGAA 
AAGCAAGATA AGACTGTCCG CAAACTGAAA AAGCAGTTGA AAGTCTTTGC CAAGAAAATT 
GGAGAGCTGG AAGTTGGTCA AATGGAAAAT GTTTCTCCTG GTCAGATAGT GGATGAACCC 
ATTCGTCCTG TTAATATTCC GCGCAAAGAG AAGGACTTCC AAGGAATGCT GGAATATAAG 
AAAGAGGACG AACTAAAGCT TGTCAAAAAC CTCATTTTAG AACTGAAGCC TCGTGGTGTC 
GCGGTCAACC TCATTCCAGG ACTGCCAGCA TATATCTTGT TCATGTGTTT GCGTCATGCT 
GATTACCTCA ATGATGATCA GAAAGTTCGC TCCTTGCTTA CATCAACTAT TAATGGTATC 
AAGAAAATCT TAAAGAAAAG AGGAGATGAT TTTGAAACGG TGTCCTTCTG GCTGTCCAAT 
TCGTGTCGGT TCCTCCATTG CTTGAAGCAG TACAGTGGAG AAGAGGGATT TATGAAGCAC 
AACACCACAC GGCAGAATGA GCACTGTCTG ACCAACTTTG ACCTTGCGGA ATACAGACAA 
GTCTTGAGTG ATCTGGCTAT TCAGATTTAC CAGCAGTTGG TCAGAGTGTT GGAAAATATC 
TTGCAGCCAA TGATTGTTTC AGGAATGCTG GAGCACGAGA CTATTCAGGG TGTCTCAGGG 
GTCAAGCCTA CCGGTCTTAG GAAGAGAACT TCCAGCATAG CAGATGAAGG GACGTACACA 
TTAGACTCCA TTGTGAGGCA GCTTAATACC TTCCATTCCA TAATGTGTCA GCATGGGATG 
GATCCTGAAC TCATCAAGCA GGTGGTCAAG CAAATGTTCT ACATCATCGG TGCTGTCACA 
CTCAACAACT TGCTCTTGCG AAAGGACATG TGCTCTTGGA GCAAAGGCAT GCAGATAAGG 
TACAACGTGA GCCAGTTGGA AGAATGGCTG CGTGACAAGA ACCTGATGAA TAGCAGCGCT 
AAAGAAACCC TGGAGCCATT AATTCAGGCT GCTCAGTTAC TGCAGGTGAA AAAGAAAACA 
GACGAAGATG CAGAAGCCAT CTGCTCCATG TGCAATGCTC TGACTACTGC CCAGATTGTC 
AAGGTGTTGA ACCTGTACAC TCCAGTAAAT GAGTTTGAAG AGCGGGTGTT GGTATCATTT 
ATACGGAATA TACAGATGCG TTTAAGGGAT AGGAAGGACT CCCCTCAGCT GCTAATGGAT 
GCTAAGCATA TCTTCCCTGT CACCTTCCCA TTCAACCCTT CGTCGCTTGC CCTAGAAACA 
ATTCAGATTC CTGGCAGTCT TGGCCTTGCC TTCCTCACAC GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755046.1
CDS	353..5920
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKKKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDDKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLFFEELYADEPMKYQSYRISLYRRIIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_031899186.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899186.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGATGA CAAGGACACA ATGACCGATT CCATGGTCCT CTCTGAGGAT 
GTACAGAAGA TGAAGGATAA AAGGGAAATT GCTCAGGCCT ACACTGGCAT GAAGGAGACA 
AATAGGGTTT TGGAATCCCA GCTGCAATCA CAGAAAAAGG CCTTTGAGAA TGAAGTGGAA 
GCTCTCAGAG GTGAGATCCA GGCTCTAAAA GAGGAGAACA ACCGGCAGCA GCAACTTTTA 
GCCCAGAACC TCCAGTTGCC ACCTGAAGCA CGAATCGAAG CCAGCTTGCA GCATGAAATC 
ACCAGGCTAA CCAACGAGAA CTTGTTTTTT GAGGAGTTAT ATGCAGATGA ACCCATGAAG 
TATCAATCGT ACCGGATCTC CCTGTACAGA CGGATCATTG ATCTGATGGA GCAACTTGAA 
AAGCAAGATA AGACTGTCCG CAAACTGAAA AAGCAGTTGA AAGTCTTTGC CAAGAAAATT 
GGAGAGCTGG AAGTTGGTCA AATGGAAAAT GTTTCTCCTG GTCAGATAGT GGATGAACCC 
ATTCGTCCTG TTAATATTCC GCGCAAAGAG AAGGACTTCC AAGGAATGCT GGAATATAAG 
AAAGAGGACG AACTAAAGCT TGTCAAAAAC CTCATTTTAG AACTGAAGCC TCGTGGTGTC 
GCGGTCAACC TCATTCCAGG ACTGCCAGCA TATATCTTGT TCATGTGTTT GCGTCATGCT 
GATTACCTCA ATGATGATCA GAAAGTTCGC TCCTTGCTTA CATCAACTAT TAATGGTATC 
AAGAAAATCT TAAAGAAAAG AGGAGATGAT TTTGAAACGG TGTCCTTCTG GCTGTCCAAT 
TCGTGTCGGT TCCTCCATTG CTTGAAGCAG TACAGTGGAG AAGAGGGATT TATGAAGCAC 
AACACCACAC GGCAGAATGA GCACTGTCTG ACCAACTTTG ACCTTGCGGA ATACAGACAA 
GTCTTGAGTG ATCTGGCTAT TCAGATTTAC CAGCAGTTGG TCAGAGTGTT GGAAAATATC 
TTGCAGCCAA TGATTGTTTC AGGAATGCTG GAGCACGAGA CTATTCAGGG TGTCTCAGGG 
GTCAAGCCTA CCGGTCTTAG GAAGAGAACT TCCAGCATAG CAGATGAAGG GACGTACACA 
TTAGACTCCA TTGTGAGGCA GCTTAATACC TTCCATTCCA TAATGTGTCA GCATGGGATG 
GATCCTGAAC TCATCAAGCA GGTGGTCAAG CAAATGTTCT ACATCATCGG TGCTGTCACA 
CTCAACAACT TGCTCTTGCG AAAGGACATG TGCTCTTGGA GCAAAGGCAT GCAGATAAGG 
TACAACGTGA GCCAGTTGGA AGAATGGCTG CGTGACAAGA ACCTGATGAA TAGCAGCGCT 
AAAGAAACCC TGGAGCCATT AATTCAGGCT GCTCAGTTAC TGCAGGTGAA AAAGAAAACA 
GACGAAGATG CAGAAGCCAT CTGCTCCATG TGCAATGCTC TGACTACTGC CCAGATTGTC 
AAGGTGTTGA ACCTGTACAC TCCAGTAAAT GAGTTTGAAG AGCGGGTGTT GGTATCATTT 
ATACGGAATA TACAGATGCG TTTAAGGGAT AGGAAGGACT CCCCTCAGCT GCTAATGGAT 
GCTAAGCATA TCTTCCCTGT CACCTTCCCA TTCAACCCTT CGTCGCTTGC CCTAGAAACA 
ATTCAGATTC CTGGCAGTCT TGGCCTTGCC TTCCTCACAC GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_031899187.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755047.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X8, mRNA.

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CloneID	OXa55096
Clone ID Related Accession (Same CDS sequence)	XM_031899187.1
Accession Version	XM_031899187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGACAC AATGACCGAT TCCATGGTCC TCTCTGAGGA TGTACAGAAG 
ATGAAGGATA AAAGGGAAAT TGCTCAGGCC TACACTGGCA TGAAGGAGAC AAATAGGGTT 
TTGGAATCCC AGCTGCAATC ACAGAAAAAG GCCTTTGAGA ATGAAGTGGA AGCTCTCAGA 
GGTGAGATCC AGGCTCTAAA AGAGGAGAAC AACCGGCAGC AGCAACTTTT AGCCCAGAAC 
CTCCAGTTGC CACCTGAAGC ACGAATCGAA GCCAGCTTGC AGCATGAAAT CACCAGGCTA 
ACCAACGAGA ACTTGTTTTT TGAGGAGTTA TATGCAGATG AACCCATGAA GTATCAATCG 
TACCGGATCT CCCTGTACAG ACGGATCATT GATCTGATGG AGCAACTTGA AAAGCAAGAT 
AAGACTGTCC GCAAACTGAA AAAGCAGTTG AAAGTCTTTG CCAAGAAAAT TGGAGAGCTG 
GAAGTTGGTC AAATGGAAAA TGTTTCTCCT GGTCAGATAG TGGATGAACC CATTCGTCCT 
GTTAATATTC CGCGCAAAGA GAAGGACTTC CAAGGAATGC TGGAATATAA GAAAGAGGAC 
GAACTAAAGC TTGTCAAAAA CCTCATTTTA GAACTGAAGC CTCGTGGTGT CGCGGTCAAC 
CTCATTCCAG GACTGCCAGC ATATATCTTG TTCATGTGTT TGCGTCATGC TGATTACCTC 
AATGATGATC AGAAAGTTCG CTCCTTGCTT ACATCAACTA TTAATGGTAT CAAGAAAATC 
TTAAAGAAAA GAGGAGATGA TTTTGAAACG GTGTCCTTCT GGCTGTCCAA TTCGTGTCGG 
TTCCTCCATT GCTTGAAGCA GTACAGTGGA GAAGAGGGAT TTATGAAGCA CAACACCACA 
CGGCAGAATG AGCACTGTCT GACCAACTTT GACCTTGCGG AATACAGACA AGTCTTGAGT 
GATCTGGCTA TTCAGATTTA CCAGCAGTTG GTCAGAGTGT TGGAAAATAT CTTGCAGCCA 
ATGATTGTTT CAGGAATGCT GGAGCACGAG ACTATTCAGG GTGTCTCAGG GGTCAAGCCT 
ACCGGTCTTA GGAAGAGAAC TTCCAGCATA GCAGATGAAG GGACGTACAC ATTAGACTCC 
ATTGTGAGGC AGCTTAATAC CTTCCATTCC ATAATGTGTC AGCATGGGAT GGATCCTGAA 
CTCATCAAGC AGGTGGTCAA GCAAATGTTC TACATCATCG GTGCTGTCAC ACTCAACAAC 
TTGCTCTTGC GAAAGGACAT GTGCTCTTGG AGCAAAGGCA TGCAGATAAG GTACAACGTG 
AGCCAGTTGG AAGAATGGCT GCGTGACAAG AACCTGATGA ATAGCAGCGC TAAAGAAACC 
CTGGAGCCAT TAATTCAGGC TGCTCAGTTA CTGCAGGTGA AAAAGAAAAC AGACGAAGAT 
GCAGAAGCCA TCTGCTCCAT GTGCAATGCT CTGACTACTG CCCAGATTGT CAAGGTGTTG 
AACCTGTACA CTCCAGTAAA TGAGTTTGAA GAGCGGGTGT TGGTATCATT TATACGGAAT 
ATACAGATGC GTTTAAGGGA TAGGAAGGAC TCCCCTCAGC TGCTAATGGA TGCTAAGCAT 
ATCTTCCCTG TCACCTTCCC ATTCAACCCT TCGTCGCTTG CCCTAGAAAC AATTCAGATT 
CCTGGCAGTC TTGGCCTTGC CTTCCTCACA CGTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755047.1
CDS	352..5910
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKKKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLFFEELYADEPMKYQSYRISLYRRIIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_031899187.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899187.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGACAC AATGACCGAT TCCATGGTCC TCTCTGAGGA TGTACAGAAG 
ATGAAGGATA AAAGGGAAAT TGCTCAGGCC TACACTGGCA TGAAGGAGAC AAATAGGGTT 
TTGGAATCCC AGCTGCAATC ACAGAAAAAG GCCTTTGAGA ATGAAGTGGA AGCTCTCAGA 
GGTGAGATCC AGGCTCTAAA AGAGGAGAAC AACCGGCAGC AGCAACTTTT AGCCCAGAAC 
CTCCAGTTGC CACCTGAAGC ACGAATCGAA GCCAGCTTGC AGCATGAAAT CACCAGGCTA 
ACCAACGAGA ACTTGTTTTT TGAGGAGTTA TATGCAGATG AACCCATGAA GTATCAATCG 
TACCGGATCT CCCTGTACAG ACGGATCATT GATCTGATGG AGCAACTTGA AAAGCAAGAT 
AAGACTGTCC GCAAACTGAA AAAGCAGTTG AAAGTCTTTG CCAAGAAAAT TGGAGAGCTG 
GAAGTTGGTC AAATGGAAAA TGTTTCTCCT GGTCAGATAG TGGATGAACC CATTCGTCCT 
GTTAATATTC CGCGCAAAGA GAAGGACTTC CAAGGAATGC TGGAATATAA GAAAGAGGAC 
GAACTAAAGC TTGTCAAAAA CCTCATTTTA GAACTGAAGC CTCGTGGTGT CGCGGTCAAC 
CTCATTCCAG GACTGCCAGC ATATATCTTG TTCATGTGTT TGCGTCATGC TGATTACCTC 
AATGATGATC AGAAAGTTCG CTCCTTGCTT ACATCAACTA TTAATGGTAT CAAGAAAATC 
TTAAAGAAAA GAGGAGATGA TTTTGAAACG GTGTCCTTCT GGCTGTCCAA TTCGTGTCGG 
TTCCTCCATT GCTTGAAGCA GTACAGTGGA GAAGAGGGAT TTATGAAGCA CAACACCACA 
CGGCAGAATG AGCACTGTCT GACCAACTTT GACCTTGCGG AATACAGACA AGTCTTGAGT 
GATCTGGCTA TTCAGATTTA CCAGCAGTTG GTCAGAGTGT TGGAAAATAT CTTGCAGCCA 
ATGATTGTTT CAGGAATGCT GGAGCACGAG ACTATTCAGG GTGTCTCAGG GGTCAAGCCT 
ACCGGTCTTA GGAAGAGAAC TTCCAGCATA GCAGATGAAG GGACGTACAC ATTAGACTCC 
ATTGTGAGGC AGCTTAATAC CTTCCATTCC ATAATGTGTC AGCATGGGAT GGATCCTGAA 
CTCATCAAGC AGGTGGTCAA GCAAATGTTC TACATCATCG GTGCTGTCAC ACTCAACAAC 
TTGCTCTTGC GAAAGGACAT GTGCTCTTGG AGCAAAGGCA TGCAGATAAG GTACAACGTG 
AGCCAGTTGG AAGAATGGCT GCGTGACAAG AACCTGATGA ATAGCAGCGC TAAAGAAACC 
CTGGAGCCAT TAATTCAGGC TGCTCAGTTA CTGCAGGTGA AAAAGAAAAC AGACGAAGAT 
GCAGAAGCCA TCTGCTCCAT GTGCAATGCT CTGACTACTG CCCAGATTGT CAAGGTGTTG 
AACCTGTACA CTCCAGTAAA TGAGTTTGAA GAGCGGGTGT TGGTATCATT TATACGGAAT 
ATACAGATGC GTTTAAGGGA TAGGAAGGAC TCCCCTCAGC TGCTAATGGA TGCTAAGCAT 
ATCTTCCCTG TCACCTTCCC ATTCAACCCT TCGTCGCTTG CCCTAGAAAC AATTCAGATT 
CCTGGCAGTC TTGGCCTTGC CTTCCTCACA CGTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_031899188.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755048.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X9, mRNA.

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CloneID	OXa55097
Clone ID Related Accession (Same CDS sequence)	XM_031899188.1
Accession Version	XM_031899188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGATGA CAAGGACACA ATGACCGATT CCATGGTCCT CTCTGAGGAT 
GTACAGAAGA TGAAGGATAA AAGGGAAATT GCTCAGGCCT ACACTGGCAT GAAGGAGACA 
AATAGGGTTT TGGAATCCCA GCTGCAATCA CAGAAAAAGG CCTTTGAGAA TGAAGTGGAA 
GCTCTCAGAG GTGAGATCCA GGCTCTAAAA GAGGAGAACA ACCGGCAGCA GCAACTTTTA 
GCCCAGAACC TCCAGTTGCC ACCTGAAGCA CGAATCGAAG CCAGCTTGCA GCATGAAATC 
ACCAGGCTAA CCAACGAGAA CTTGGATCTG ATGGAGCAAC TTGAAAAGCA AGATAAGACT 
GTCCGCAAAC TGAAAAAGCA GTTGAAAGTC TTTGCCAAGA AAATTGGAGA GCTGGAAGTT 
GGTCAAATGG AAAATGTTTC TCCTGGTCAG ATAGTGGATG AACCCATTCG TCCTGTTAAT 
ATTCCGCGCA AAGAGAAGGA CTTCCAAGGA ATGCTGGAAT ATAAGAAAGA GGACGAACTA 
AAGCTTGTCA AAAACCTCAT TTTAGAACTG AAGCCTCGTG GTGTCGCGGT CAACCTCATT 
CCAGGACTGC CAGCATATAT CTTGTTCATG TGTTTGCGTC ATGCTGATTA CCTCAATGAT 
GATCAGAAAG TTCGCTCCTT GCTTACATCA ACTATTAATG GTATCAAGAA AATCTTAAAG 
AAAAGAGGAG ATGATTTTGA AACGGTGTCC TTCTGGCTGT CCAATTCGTG TCGGTTCCTC 
CATTGCTTGA AGCAGTACAG TGGAGAAGAG GGATTTATGA AGCACAACAC CACACGGCAG 
AATGAGCACT GTCTGACCAA CTTTGACCTT GCGGAATACA GACAAGTCTT GAGTGATCTG 
GCTATTCAGA TTTACCAGCA GTTGGTCAGA GTGTTGGAAA ATATCTTGCA GCCAATGATT 
GTTTCAGGAA TGCTGGAGCA CGAGACTATT CAGGGTGTCT CAGGGGTCAA GCCTACCGGT 
CTTAGGAAGA GAACTTCCAG CATAGCAGAT GAAGGGACGT ACACATTAGA CTCCATTGTG 
AGGCAGCTTA ATACCTTCCA TTCCATAATG TGTCAGCATG GGATGGATCC TGAACTCATC 
AAGCAGGTGG TCAAGCAAAT GTTCTACATC ATCGGTGCTG TCACACTCAA CAACTTGCTC 
TTGCGAAAGG ACATGTGCTC TTGGAGCAAA GGCATGCAGA TAAGGTACAA CGTGAGCCAG 
TTGGAAGAAT GGCTGCGTGA CAAGAACCTG ATGAATAGCA GCGCTAAAGA AACCCTGGAG 
CCATTAATTC AGGCTGCTCA GTTACTGCAG GTGAAAAAGA AAACAGACGA AGATGCAGAA 
GCCATCTGCT CCATGTGCAA TGCTCTGACT ACTGCCCAGA TTGTCAAGGT GTTGAACCTG 
TACACTCCAG TAAATGAGTT TGAAGAGCGG GTGTTGGTAT CATTTATACG GAATATACAG 
ATGCGTTTAA GGGATAGGAA GGACTCCCCT CAGCTGCTAA TGGATGCTAA GCATATCTTC 
CCTGTCACCT TCCCATTCAA CCCTTCGTCG CTTGCCCTAG AAACAATTCA GATTCCTGGC 
AGTCTTGGCC TTGCCTTCCT CACACGTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755048.1
CDS	352..5844
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKKKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDDKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_031899188.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899188.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGATGA CAAGGACACA ATGACCGATT CCATGGTCCT CTCTGAGGAT 
GTACAGAAGA TGAAGGATAA AAGGGAAATT GCTCAGGCCT ACACTGGCAT GAAGGAGACA 
AATAGGGTTT TGGAATCCCA GCTGCAATCA CAGAAAAAGG CCTTTGAGAA TGAAGTGGAA 
GCTCTCAGAG GTGAGATCCA GGCTCTAAAA GAGGAGAACA ACCGGCAGCA GCAACTTTTA 
GCCCAGAACC TCCAGTTGCC ACCTGAAGCA CGAATCGAAG CCAGCTTGCA GCATGAAATC 
ACCAGGCTAA CCAACGAGAA CTTGGATCTG ATGGAGCAAC TTGAAAAGCA AGATAAGACT 
GTCCGCAAAC TGAAAAAGCA GTTGAAAGTC TTTGCCAAGA AAATTGGAGA GCTGGAAGTT 
GGTCAAATGG AAAATGTTTC TCCTGGTCAG ATAGTGGATG AACCCATTCG TCCTGTTAAT 
ATTCCGCGCA AAGAGAAGGA CTTCCAAGGA ATGCTGGAAT ATAAGAAAGA GGACGAACTA 
AAGCTTGTCA AAAACCTCAT TTTAGAACTG AAGCCTCGTG GTGTCGCGGT CAACCTCATT 
CCAGGACTGC CAGCATATAT CTTGTTCATG TGTTTGCGTC ATGCTGATTA CCTCAATGAT 
GATCAGAAAG TTCGCTCCTT GCTTACATCA ACTATTAATG GTATCAAGAA AATCTTAAAG 
AAAAGAGGAG ATGATTTTGA AACGGTGTCC TTCTGGCTGT CCAATTCGTG TCGGTTCCTC 
CATTGCTTGA AGCAGTACAG TGGAGAAGAG GGATTTATGA AGCACAACAC CACACGGCAG 
AATGAGCACT GTCTGACCAA CTTTGACCTT GCGGAATACA GACAAGTCTT GAGTGATCTG 
GCTATTCAGA TTTACCAGCA GTTGGTCAGA GTGTTGGAAA ATATCTTGCA GCCAATGATT 
GTTTCAGGAA TGCTGGAGCA CGAGACTATT CAGGGTGTCT CAGGGGTCAA GCCTACCGGT 
CTTAGGAAGA GAACTTCCAG CATAGCAGAT GAAGGGACGT ACACATTAGA CTCCATTGTG 
AGGCAGCTTA ATACCTTCCA TTCCATAATG TGTCAGCATG GGATGGATCC TGAACTCATC 
AAGCAGGTGG TCAAGCAAAT GTTCTACATC ATCGGTGCTG TCACACTCAA CAACTTGCTC 
TTGCGAAAGG ACATGTGCTC TTGGAGCAAA GGCATGCAGA TAAGGTACAA CGTGAGCCAG 
TTGGAAGAAT GGCTGCGTGA CAAGAACCTG ATGAATAGCA GCGCTAAAGA AACCCTGGAG 
CCATTAATTC AGGCTGCTCA GTTACTGCAG GTGAAAAAGA AAACAGACGA AGATGCAGAA 
GCCATCTGCT CCATGTGCAA TGCTCTGACT ACTGCCCAGA TTGTCAAGGT GTTGAACCTG 
TACACTCCAG TAAATGAGTT TGAAGAGCGG GTGTTGGTAT CATTTATACG GAATATACAG 
ATGCGTTTAA GGGATAGGAA GGACTCCCCT CAGCTGCTAA TGGATGCTAA GCATATCTTC 
CCTGTCACCT TCCCATTCAA CCCTTCGTCG CTTGCCCTAG AAACAATTCA GATTCCTGGC 
AGTCTTGGCC TTGCCTTCCT CACACGTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_031899189.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755049.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X10, mRNA.

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CloneID	OXa55098
Clone ID Related Accession (Same CDS sequence)	XM_031899189.1
Accession Version	XM_031899189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGACAC AATGACCGAT TCCATGGTCC TCTCTGAGGA TGTACAGAAG 
ATGAAGGATA AAAGGGAAAT TGCTCAGGCC TACACTGGCA TGAAGGAGAC AAATAGGGTT 
TTGGAATCCC AGCTGCAATC ACAGAAAAAG GCCTTTGAGA ATGAAGTGGA AGCTCTCAGA 
GGTGAGATCC AGGCTCTAAA AGAGGAGAAC AACCGGCAGC AGCAACTTTT AGCCCAGAAC 
CTCCAGTTGC CACCTGAAGC ACGAATCGAA GCCAGCTTGC AGCATGAAAT CACCAGGCTA 
ACCAACGAGA ACTTGGATCT GATGGAGCAA CTTGAAAAGC AAGATAAGAC TGTCCGCAAA 
CTGAAAAAGC AGTTGAAAGT CTTTGCCAAG AAAATTGGAG AGCTGGAAGT TGGTCAAATG 
GAAAATGTTT CTCCTGGTCA GATAGTGGAT GAACCCATTC GTCCTGTTAA TATTCCGCGC 
AAAGAGAAGG ACTTCCAAGG AATGCTGGAA TATAAGAAAG AGGACGAACT AAAGCTTGTC 
AAAAACCTCA TTTTAGAACT GAAGCCTCGT GGTGTCGCGG TCAACCTCAT TCCAGGACTG 
CCAGCATATA TCTTGTTCAT GTGTTTGCGT CATGCTGATT ACCTCAATGA TGATCAGAAA 
GTTCGCTCCT TGCTTACATC AACTATTAAT GGTATCAAGA AAATCTTAAA GAAAAGAGGA 
GATGATTTTG AAACGGTGTC CTTCTGGCTG TCCAATTCGT GTCGGTTCCT CCATTGCTTG 
AAGCAGTACA GTGGAGAAGA GGGATTTATG AAGCACAACA CCACACGGCA GAATGAGCAC 
TGTCTGACCA ACTTTGACCT TGCGGAATAC AGACAAGTCT TGAGTGATCT GGCTATTCAG 
ATTTACCAGC AGTTGGTCAG AGTGTTGGAA AATATCTTGC AGCCAATGAT TGTTTCAGGA 
ATGCTGGAGC ACGAGACTAT TCAGGGTGTC TCAGGGGTCA AGCCTACCGG TCTTAGGAAG 
AGAACTTCCA GCATAGCAGA TGAAGGGACG TACACATTAG ACTCCATTGT GAGGCAGCTT 
AATACCTTCC ATTCCATAAT GTGTCAGCAT GGGATGGATC CTGAACTCAT CAAGCAGGTG 
GTCAAGCAAA TGTTCTACAT CATCGGTGCT GTCACACTCA ACAACTTGCT CTTGCGAAAG 
GACATGTGCT CTTGGAGCAA AGGCATGCAG ATAAGGTACA ACGTGAGCCA GTTGGAAGAA 
TGGCTGCGTG ACAAGAACCT GATGAATAGC AGCGCTAAAG AAACCCTGGA GCCATTAATT 
CAGGCTGCTC AGTTACTGCA GGTGAAAAAG AAAACAGACG AAGATGCAGA AGCCATCTGC 
TCCATGTGCA ATGCTCTGAC TACTGCCCAG ATTGTCAAGG TGTTGAACCT GTACACTCCA 
GTAAATGAGT TTGAAGAGCG GGTGTTGGTA TCATTTATAC GGAATATACA GATGCGTTTA 
AGGGATAGGA AGGACTCCCC TCAGCTGCTA ATGGATGCTA AGCATATCTT CCCTGTCACC 
TTCCCATTCA ACCCTTCGTC GCTTGCCCTA GAAACAATTC AGATTCCTGG CAGTCTTGGC 
CTTGCCTTCC TCACACGTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755049.1
CDS	351..5834
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKKKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_031899189.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899189.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGAAGTT TACACTGCTA ACAGAACTGT TCCAAGAAGA GGAGCAGATC 
TTGAGCCCTA CATCTTCTGC ACCACCCTCA GGCCGTACTC TGCTATCCCG CACTGGTCTG 
CGTTCCCTCA AGCCCAAGCC TGAACAGACA AGCAAGGAGC ATAAAAAAAC TGTAGGACAT 
CAGTTCAGAA ATTCCCTTCA CCTTCTGATG GAGACTCTCA ATGCCACAAC TCCACATTAT 
GTCCGTTGCA TTAAGCCAAA TGACTTCAAG TACCCGTTCA CATTTGACTC TAAACGAGCA 
GTGCAGCAGC TTAGGGCTTG TGGCGTCCTG GAAACCATCC GAATCAGTGC TGCTGGATTT 
CCTTCTAGGT GGACATATCA GGAATTTTTT AGCAGATATC GTGTTTTAAT GAAGCAAAAA 
GATGTCCTCA GTGATAGGAA GCAAACCTGC AAAAATGTTC TGGAGAAGCT TATTTTGGAT 
AAGGATAAGT ACCAGTTTGG TAAGACAAAG ATTTTTTTCC GTGCTGGCCA AGTGGCCTAT 
CTGGAGAAGA TCCGAGCAGA CAAGCTGAGA ATGGCCTGTA TCAGGATCCA GAAGACAATC 
CGAGGATGGC TTTTGAGGAA GAAATACTTG CGCATGAGGA AAGCAGCCAT AACAATCCAG 
CGCTACGTCA GAGGATACCA GGCACGATGT TATGCACAGT TTCTGCGTAG AACACAGGCC 
GCGATTATTA TCCAGAAATT CCAAAGGATG TATGTCGTTC GGCAAAAGTA CCGTCATATG 
CAGTCCATTA CCCTTGCTCT CCAGTCGTAT ATGCGTGGTT ATGCAGCCAG GAAGAGATTT 
CAGGGGATGC TTAGAGCACA CAAAGCTACC ATCATCCAGA AGCATGTGCG CGGCTGGTTA 
GCCCGTGTCA CTTATAAGAG AAATTTGAGT GCTATTGTGT ACCTGCAGTG TTGTTACCGC 
AGGATGATGG CCAAAAGGGA GCTGAAGAAG TTAAAGATAG AGGCACGCTC AGTGGAACAC 
TACAAGAAAC TCAACAATGG GATGGAAAAC AAGATCATGC AGTTGCAGCG TAAAGTTGAT 
GAGCAGAATA AAGACAACAA ATCCTTGCTG GAGAGGCTAA CACACCTGGA GGTCACGTAC 
AATACTGAAA AAGATAAGCT GAGAAGCGAC GTCGACAGAC TGCGCCATTT TGAAGAGGAG 
GCCAAAAATG CAGCAAACAG GATGGTCAGC TTGCAGGACG AGCTTGCCAG GCTTAGGAAA 
GAGCTGCAGC AAACACAGAC GGAAAAGAAC AAGATTGAGG AACGGGCAGA GAAGTACCAG 
ACCGAGACTG AAAAGCTGGT TGCTGAGCTG AGAGAACAGA ATGCTTTGCT GAAAGCTGAG 
AAAGAGGAAC TGAATCTTTT AATCCAGGAG CAGGCAAAAA AAATGACAGA GGATATGGAA 
AAGAAAATTA TAGAAGAAAC AAAGCAGTTG GAGCTTGAGC TGAATGATGA ACGACTGAGG 
TACCAGAATC TCCTAAACGA GTACAGCCGC CTGGAGGAGC GATATGATGA CCTTAAAGAT 
GAAATTAATA CCATGAGTCC TCCAAAGCCA GGACACAAGC GCACAGACTC TACTCATAGC 
AGCAATGAAT CAGAATACAC TTTTAGTTCA GAGATCACAG AGTCAGAAGA TTACCCATAT 
CGAAATGAGG AGCCTGCTGA CAGAAAAACT CCGCTGGACA TGTCCCTGTT CCTTAAGCTT 
CAGAAACGGG TAAAGGAACT TGAGCAGGAA AAGCAAGTAA TGCAAGATGA TTTGGATAAG 
AAGGAGGAGC AGATAATCCG AGCCAAAATA ATGGAGGAGG CAAAGACTCC AACTAGAGGT 
ACAGAACTGG AATATGAATC ACTCAAGCGT CAAGAACTGG AATCTGAAAA TAAAAAACTT 
AAGAATGAGT TAAATGAGCT GCGAAGGGCA ATCACTGAGA AAGCCTCCCC AGATGCTACA 
GGCCCTGGTG GAACTGTCTA TAAAGTTCTC TTGGAGCAGA TGACGTCAGT AAGTGAGGAA 
CTTGAAGTAC GCAAGGAAGA AGTCCTTATA CTGAGGTCCC AGTTGGTGAG CCAAATGGAA 
GCCATTCCAC ACAAGGACAC AATGACCGAT TCCATGGTCC TCTCTGAGGA TGTACAGAAG 
ATGAAGGATA AAAGGGAAAT TGCTCAGGCC TACACTGGCA TGAAGGAGAC AAATAGGGTT 
TTGGAATCCC AGCTGCAATC ACAGAAAAAG GCCTTTGAGA ATGAAGTGGA AGCTCTCAGA 
GGTGAGATCC AGGCTCTAAA AGAGGAGAAC AACCGGCAGC AGCAACTTTT AGCCCAGAAC 
CTCCAGTTGC CACCTGAAGC ACGAATCGAA GCCAGCTTGC AGCATGAAAT CACCAGGCTA 
ACCAACGAGA ACTTGGATCT GATGGAGCAA CTTGAAAAGC AAGATAAGAC TGTCCGCAAA 
CTGAAAAAGC AGTTGAAAGT CTTTGCCAAG AAAATTGGAG AGCTGGAAGT TGGTCAAATG 
GAAAATGTTT CTCCTGGTCA GATAGTGGAT GAACCCATTC GTCCTGTTAA TATTCCGCGC 
AAAGAGAAGG ACTTCCAAGG AATGCTGGAA TATAAGAAAG AGGACGAACT AAAGCTTGTC 
AAAAACCTCA TTTTAGAACT GAAGCCTCGT GGTGTCGCGG TCAACCTCAT TCCAGGACTG 
CCAGCATATA TCTTGTTCAT GTGTTTGCGT CATGCTGATT ACCTCAATGA TGATCAGAAA 
GTTCGCTCCT TGCTTACATC AACTATTAAT GGTATCAAGA AAATCTTAAA GAAAAGAGGA 
GATGATTTTG AAACGGTGTC CTTCTGGCTG TCCAATTCGT GTCGGTTCCT CCATTGCTTG 
AAGCAGTACA GTGGAGAAGA GGGATTTATG AAGCACAACA CCACACGGCA GAATGAGCAC 
TGTCTGACCA ACTTTGACCT TGCGGAATAC AGACAAGTCT TGAGTGATCT GGCTATTCAG 
ATTTACCAGC AGTTGGTCAG AGTGTTGGAA AATATCTTGC AGCCAATGAT TGTTTCAGGA 
ATGCTGGAGC ACGAGACTAT TCAGGGTGTC TCAGGGGTCA AGCCTACCGG TCTTAGGAAG 
AGAACTTCCA GCATAGCAGA TGAAGGGACG TACACATTAG ACTCCATTGT GAGGCAGCTT 
AATACCTTCC ATTCCATAAT GTGTCAGCAT GGGATGGATC CTGAACTCAT CAAGCAGGTG 
GTCAAGCAAA TGTTCTACAT CATCGGTGCT GTCACACTCA ACAACTTGCT CTTGCGAAAG 
GACATGTGCT CTTGGAGCAA AGGCATGCAG ATAAGGTACA ACGTGAGCCA GTTGGAAGAA 
TGGCTGCGTG ACAAGAACCT GATGAATAGC AGCGCTAAAG AAACCCTGGA GCCATTAATT 
CAGGCTGCTC AGTTACTGCA GGTGAAAAAG AAAACAGACG AAGATGCAGA AGCCATCTGC 
TCCATGTGCA ATGCTCTGAC TACTGCCCAG ATTGTCAAGG TGTTGAACCT GTACACTCCA 
GTAAATGAGT TTGAAGAGCG GGTGTTGGTA TCATTTATAC GGAATATACA GATGCGTTTA 
AGGGATAGGA AGGACTCCCC TCAGCTGCTA ATGGATGCTA AGCATATCTT CCCTGTCACC 
TTCCCATTCA ACCCTTCGTC GCTTGCCCTA GAAACAATTC AGATTCCTGG CAGTCTTGGC 
CTTGCCTTCC TCACACGTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_004912697.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912754.2 Xenopus tropicalis myosin VA (myo5a), transcript variant X11, mRNA.

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CloneID	OXa55099
Clone ID Related Accession (Same CDS sequence)	XM_004912697.4
Accession Version	XM_004912697.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912697.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACA GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAAA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ACACAATGAC CGATTCCATG GTCCTCTCTG AGGATGTACA GAAGATGAAG 
GATAAAAGGG AAATTGCTCA GGCCTACACT GGCATGAAGG AGACAAATAG GGTTTTGGAA 
TCCCAGCTGC AATCACAGAA AAAGGCCTTT GAGAATGAAG TGGAAGCTCT CAGAGGTGAG 
ATCCAGGCTC TAAAAGAGGA GAACAACCGG CAGCAGCAAC TTTTAGCCCA GAACCTCCAG 
TTGCCACCTG AAGCACGAAT CGAAGCCAGC TTGCAGCATG AAATCACCAG GCTAACCAAC 
GAGAACTTGG ATCTGATGGA GCAACTTGAA AAGCAAGATA AGACTGTCCG CAAACTGAAA 
AAGCAGTTGA AAGTCTTTGC CAAGAAAATT GGAGAGCTGG AAGTTGGTCA AATGGAAAAT 
GTTTCTCCTG GTCAGATAGT GGATGAACCC ATTCGTCCTG TTAATATTCC GCGCAAAGAG 
AAGGACTTCC AAGGAATGCT GGAATATAAG AAAGAGGACG AACTAAAGCT TGTCAAAAAC 
CTCATTTTAG AACTGAAGCC TCGTGGTGTC GCGGTCAACC TCATTCCAGG ACTGCCAGCA 
TATATCTTGT TCATGTGTTT GCGTCATGCT GATTACCTCA ATGATGATCA GAAAGTTCGC 
TCCTTGCTTA CATCAACTAT TAATGGTATC AAGAAAATCT TAAAGAAAAG AGGAGATGAT 
TTTGAAACGG TGTCCTTCTG GCTGTCCAAT TCGTGTCGGT TCCTCCATTG CTTGAAGCAG 
TACAGTGGAG AAGAGGGATT TATGAAGCAC AACACCACAC GGCAGAATGA GCACTGTCTG 
ACCAACTTTG ACCTTGCGGA ATACAGACAA GTCTTGAGTG ATCTGGCTAT TCAGATTTAC 
CAGCAGTTGG TCAGAGTGTT GGAAAATATC TTGCAGCCAA TGATTGTTTC AGGAATGCTG 
GAGCACGAGA CTATTCAGGG TGTCTCAGGG GTCAAGCCTA CCGGTCTTAG GAAGAGAACT 
TCCAGCATAG CAGATGAAGG GACGTACACA TTAGACTCCA TTGTGAGGCA GCTTAATACC 
TTCCATTCCA TAATGTGTCA GCATGGGATG GATCCTGAAC TCATCAAGCA GGTGGTCAAG 
CAAATGTTCT ACATCATCGG TGCTGTCACA CTCAACAACT TGCTCTTGCG AAAGGACATG 
TGCTCTTGGA GCAAAGGCAT GCAGATAAGG TACAACGTGA GCCAGTTGGA AGAATGGCTG 
CGTGACAAGA ACCTGATGAA TAGCAGCGCT AAAGAAACCC TGGAGCCATT AATTCAGGCT 
GCTCAGTTAC TGCAGGTGAA AAAGAAAACA GACGAAGATG CAGAAGCCAT CTGCTCCATG 
TGCAATGCTC TGACTACTGC CCAGATTGTC AAGGTGTTGA ACCTGTACAC TCCAGTAAAT 
GAGTTTGAAG AGCGGGTGTT GGTATCATTT ATACGGAATA TACAGATGCG TTTAAGGGAT 
AGGAAGGACT CCCCTCAGCT GCTAATGGAT GCTAAGCATA TCTTCCCTGT CACCTTCCCA 
TTCAACCCTT CGTCGCTTGC CCTAGAAACA ATTCAGATTC CTGGCAGTCT TGGCCTTGCC 
TTCCTCACAC GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912754.2
CDS	351..5828
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_004912697.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912697.4

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACA GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAAA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ACACAATGAC CGATTCCATG GTCCTCTCTG AGGATGTACA GAAGATGAAG 
GATAAAAGGG AAATTGCTCA GGCCTACACT GGCATGAAGG AGACAAATAG GGTTTTGGAA 
TCCCAGCTGC AATCACAGAA AAAGGCCTTT GAGAATGAAG TGGAAGCTCT CAGAGGTGAG 
ATCCAGGCTC TAAAAGAGGA GAACAACCGG CAGCAGCAAC TTTTAGCCCA GAACCTCCAG 
TTGCCACCTG AAGCACGAAT CGAAGCCAGC TTGCAGCATG AAATCACCAG GCTAACCAAC 
GAGAACTTGG ATCTGATGGA GCAACTTGAA AAGCAAGATA AGACTGTCCG CAAACTGAAA 
AAGCAGTTGA AAGTCTTTGC CAAGAAAATT GGAGAGCTGG AAGTTGGTCA AATGGAAAAT 
GTTTCTCCTG GTCAGATAGT GGATGAACCC ATTCGTCCTG TTAATATTCC GCGCAAAGAG 
AAGGACTTCC AAGGAATGCT GGAATATAAG AAAGAGGACG AACTAAAGCT TGTCAAAAAC 
CTCATTTTAG AACTGAAGCC TCGTGGTGTC GCGGTCAACC TCATTCCAGG ACTGCCAGCA 
TATATCTTGT TCATGTGTTT GCGTCATGCT GATTACCTCA ATGATGATCA GAAAGTTCGC 
TCCTTGCTTA CATCAACTAT TAATGGTATC AAGAAAATCT TAAAGAAAAG AGGAGATGAT 
TTTGAAACGG TGTCCTTCTG GCTGTCCAAT TCGTGTCGGT TCCTCCATTG CTTGAAGCAG 
TACAGTGGAG AAGAGGGATT TATGAAGCAC AACACCACAC GGCAGAATGA GCACTGTCTG 
ACCAACTTTG ACCTTGCGGA ATACAGACAA GTCTTGAGTG ATCTGGCTAT TCAGATTTAC 
CAGCAGTTGG TCAGAGTGTT GGAAAATATC TTGCAGCCAA TGATTGTTTC AGGAATGCTG 
GAGCACGAGA CTATTCAGGG TGTCTCAGGG GTCAAGCCTA CCGGTCTTAG GAAGAGAACT 
TCCAGCATAG CAGATGAAGG GACGTACACA TTAGACTCCA TTGTGAGGCA GCTTAATACC 
TTCCATTCCA TAATGTGTCA GCATGGGATG GATCCTGAAC TCATCAAGCA GGTGGTCAAG 
CAAATGTTCT ACATCATCGG TGCTGTCACA CTCAACAACT TGCTCTTGCG AAAGGACATG 
TGCTCTTGGA GCAAAGGCAT GCAGATAAGG TACAACGTGA GCCAGTTGGA AGAATGGCTG 
CGTGACAAGA ACCTGATGAA TAGCAGCGCT AAAGAAACCC TGGAGCCATT AATTCAGGCT 
GCTCAGTTAC TGCAGGTGAA AAAGAAAACA GACGAAGATG CAGAAGCCAT CTGCTCCATG 
TGCAATGCTC TGACTACTGC CCAGATTGTC AAGGTGTTGA ACCTGTACAC TCCAGTAAAT 
GAGTTTGAAG AGCGGGTGTT GGTATCATTT ATACGGAATA TACAGATGCG TTTAAGGGAT 
AGGAAGGACT CCCCTCAGCT GCTAATGGAT GCTAAGCATA TCTTCCCTGT CACCTTCCCA 
TTCAACCCTT CGTCGCTTGC CCTAGAAACA ATTCAGATTC CTGGCAGTCT TGGCCTTGCC 
TTCCTCACAC GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_031899190.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755050.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X12, mRNA.

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CloneID	OXa55100
Clone ID Related Accession (Same CDS sequence)	XM_031899190.1
Accession Version	XM_031899190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACA GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAAA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ATGACAAGGA CACAATGACC GATTCCATGG TCCTCTCTGA GGATGTACAG 
AAGATGAAGG ATAAAAGGGA AATTGCTCAG GCCTACACTG GCATGAAGGA GACAAATAGT 
CTTGAAACTA TTGAAAGAAA CCTGCCTCCA GACTACCAAG CCCTAAATGA GGACGGAGAG 
CTGTGGCTGG TTTATGATGG GTTAAAACAG GCCAACAGGG TTTTGGAATC CCAGCTGCAA 
TCACAGAAAA AGGCCTTTGA GAATGAAGTG GAAGCTCTCA GAGGTGAGAT CCAGGCTCTA 
AAAGAGGAGA ACAACCGGCA GCAGCAACTT TTAGCCCAGA ACCTCCAGTT GCCACCTGAA 
GCACGAATCG AAGCCAGCTT GCAGCATGAA ATCACCAGGC TAACCAACGA GAACTTGGAT 
CTGATGGAGC AACTTGAAAA GCAAGATAAG ACTGTCCGCA AACTGAAAAA GCAGTTGAAA 
GTCTTTGCCA AGAAAATTGG AGAGCTGGAA GTTGGTCAAA TGGAAAATGT TTCTCCTGGT 
CAGATAGTGG ATGAACCCAT TCGTCCTGTT AATATTCCGC GCAAAGAGAA GGACTTCCAA 
GGAATGCTGG AATATAAGAA AGAGGACGAA CTAAAGCTTG TCAAAAACCT CATTTTAGAA 
CTGAAGCCTC GTGGTGTCGC GGTCAACCTC ATTCCAGGAC TGCCAGCATA TATCTTGTTC 
ATGTGTTTGC GTCATGCTGA TTACCTCAAT GATGATCAGA AAGTTCGCTC CTTGCTTACA 
TCAACTATTA ATGGTATCAA GAAAATCTTA AAGAAAAGAG GAGATGATTT TGAAACGGTG 
TCCTTCTGGC TGTCCAATTC GTGTCGGTTC CTCCATTGCT TGAAGCAGTA CAGTGGAGAA 
GAGGGATTTA TGAAGCACAA CACCACACGG CAGAATGAGC ACTGTCTGAC CAACTTTGAC 
CTTGCGGAAT ACAGACAAGT CTTGAGTGAT CTGGCTATTC AGATTTACCA GCAGTTGGTC 
AGAGTGTTGG AAAATATCTT GCAGCCAATG ATTGTTTCAG GAATGCTGGA GCACGAGACT 
ATTCAGGGTG TCTCAGGGGT CAAGCCTACC GGTCTTAGGA AGAGAACTTC CAGCATAGCA 
GATGAAGGGA CGTACACATT AGACTCCATT GTGAGGCAGC TTAATACCTT CCATTCCATA 
ATGTGTCAGC ATGGGATGGA TCCTGAACTC ATCAAGCAGG TGGTCAAGCA AATGTTCTAC 
ATCATCGGTG CTGTCACACT CAACAACTTG CTCTTGCGAA AGGACATGTG CTCTTGGAGC 
AAAGGCATGC AGATAAGGTA CAACGTGAGC CAGTTGGAAG AATGGCTGCG TGACAAGAAC 
CTGATGAATA GCAGCGCTAA AGAAACCCTG GAGCCATTAA TTCAGGCTGC TCAGTTACTG 
CAGGTGAAAA AGAAAACAGA CGAAGATGCA GAAGCCATCT GCTCCATGTG CAATGCTCTG 
ACTACTGCCC AGATTGTCAA GGTGTTGAAC CTGTACACTC CAGTAAATGA GTTTGAAGAG 
CGGGTGTTGG TATCATTTAT ACGGAATATA CAGATGCGTT TAAGGGATAG GAAGGACTCC 
CCTCAGCTGC TAATGGATGC TAAGCATATC TTCCCTGTCA CCTTCCCATT CAACCCTTCG 
TCGCTTGCCC TAGAAACAAT TCAGATTCCT GGCAGTCTTG GCCTTGCCTT CCTCACACGT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755050.1
CDS	353..5938
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDDKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNSLETIERNLPPDYQALNEDGELWLVYDGLKQANRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_031899190.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899190.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACA GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAAA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ATGACAAGGA CACAATGACC GATTCCATGG TCCTCTCTGA GGATGTACAG 
AAGATGAAGG ATAAAAGGGA AATTGCTCAG GCCTACACTG GCATGAAGGA GACAAATAGT 
CTTGAAACTA TTGAAAGAAA CCTGCCTCCA GACTACCAAG CCCTAAATGA GGACGGAGAG 
CTGTGGCTGG TTTATGATGG GTTAAAACAG GCCAACAGGG TTTTGGAATC CCAGCTGCAA 
TCACAGAAAA AGGCCTTTGA GAATGAAGTG GAAGCTCTCA GAGGTGAGAT CCAGGCTCTA 
AAAGAGGAGA ACAACCGGCA GCAGCAACTT TTAGCCCAGA ACCTCCAGTT GCCACCTGAA 
GCACGAATCG AAGCCAGCTT GCAGCATGAA ATCACCAGGC TAACCAACGA GAACTTGGAT 
CTGATGGAGC AACTTGAAAA GCAAGATAAG ACTGTCCGCA AACTGAAAAA GCAGTTGAAA 
GTCTTTGCCA AGAAAATTGG AGAGCTGGAA GTTGGTCAAA TGGAAAATGT TTCTCCTGGT 
CAGATAGTGG ATGAACCCAT TCGTCCTGTT AATATTCCGC GCAAAGAGAA GGACTTCCAA 
GGAATGCTGG AATATAAGAA AGAGGACGAA CTAAAGCTTG TCAAAAACCT CATTTTAGAA 
CTGAAGCCTC GTGGTGTCGC GGTCAACCTC ATTCCAGGAC TGCCAGCATA TATCTTGTTC 
ATGTGTTTGC GTCATGCTGA TTACCTCAAT GATGATCAGA AAGTTCGCTC CTTGCTTACA 
TCAACTATTA ATGGTATCAA GAAAATCTTA AAGAAAAGAG GAGATGATTT TGAAACGGTG 
TCCTTCTGGC TGTCCAATTC GTGTCGGTTC CTCCATTGCT TGAAGCAGTA CAGTGGAGAA 
GAGGGATTTA TGAAGCACAA CACCACACGG CAGAATGAGC ACTGTCTGAC CAACTTTGAC 
CTTGCGGAAT ACAGACAAGT CTTGAGTGAT CTGGCTATTC AGATTTACCA GCAGTTGGTC 
AGAGTGTTGG AAAATATCTT GCAGCCAATG ATTGTTTCAG GAATGCTGGA GCACGAGACT 
ATTCAGGGTG TCTCAGGGGT CAAGCCTACC GGTCTTAGGA AGAGAACTTC CAGCATAGCA 
GATGAAGGGA CGTACACATT AGACTCCATT GTGAGGCAGC TTAATACCTT CCATTCCATA 
ATGTGTCAGC ATGGGATGGA TCCTGAACTC ATCAAGCAGG TGGTCAAGCA AATGTTCTAC 
ATCATCGGTG CTGTCACACT CAACAACTTG CTCTTGCGAA AGGACATGTG CTCTTGGAGC 
AAAGGCATGC AGATAAGGTA CAACGTGAGC CAGTTGGAAG AATGGCTGCG TGACAAGAAC 
CTGATGAATA GCAGCGCTAA AGAAACCCTG GAGCCATTAA TTCAGGCTGC TCAGTTACTG 
CAGGTGAAAA AGAAAACAGA CGAAGATGCA GAAGCCATCT GCTCCATGTG CAATGCTCTG 
ACTACTGCCC AGATTGTCAA GGTGTTGAAC CTGTACACTC CAGTAAATGA GTTTGAAGAG 
CGGGTGTTGG TATCATTTAT ACGGAATATA CAGATGCGTT TAAGGGATAG GAAGGACTCC 
CCTCAGCTGC TAATGGATGC TAAGCATATC TTCCCTGTCA CCTTCCCATT CAACCCTTCG 
TCGCTTGCCC TAGAAACAAT TCAGATTCCT GGCAGTCTTG GCCTTGCCTT CCTCACACGT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_031899191.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755051.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X13, mRNA.

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CloneID	OXa55101
Clone ID Related Accession (Same CDS sequence)	XM_031899191.1
Accession Version	XM_031899191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGTTTAC ACTGCTAACA GAACTGTTCC AAGAAGAGGA GCAGATCTTG 
AGCCCTACAT CTTCTGCACC ACCCTCAGGC CGTACTCTGC TATCCCGCAC TGGTCTGCGT 
TCCCTCAAGC CCAAGCCTGA ACAGACAAGC AAGGAGCATA AAAAAACTGT AGGACATCAG 
TTCAGAAATT CCCTTCACCT TCTGATGGAG ACTCTCAATG CCACAACTCC ACATTATGTC 
CGTTGCATTA AGCCAAATGA CTTCAAGTAC CCGTTCACAT TTGACTCTAA ACGAGCAGTG 
CAGCAGCTTA GGGCTTGTGG CGTCCTGGAA ACCATCCGAA TCAGTGCTGC TGGATTTCCT 
TCTAGGTGGA CATATCAGGA ATTTTTTAGC AGATATCGTG TTTTAATGAA GCAAAAAGAT 
GTCCTCAGTG ATAGGAAGCA AACCTGCAAA AATGTTCTGG AGAAGCTTAT TTTGGATAAG 
GATAAGTACC AGTTTGGTAA GACAAAGATT TTTTTCCGTG CTGGCCAAGT GGCCTATCTG 
GAGAAGATCC GAGCAGACAA GCTGAGAATG GCCTGTATCA GGATCCAGAA GACAATCCGA 
GGATGGCTTT TGAGGAAGAA ATACTTGCGC ATGAGGAAAG CAGCCATAAC AATCCAGCGC 
TACGTCAGAG GATACCAGGC ACGATGTTAT GCACAGTTTC TGCGTAGAAC ACAGGCCGCG 
ATTATTATCC AGAAATTCCA AAGGATGTAT GTCGTTCGGC AAAAGTACCG TCATATGCAG 
TCCATTACCC TTGCTCTCCA GTCGTATATG CGTGGTTATG CAGCCAGGAA GAGATTTCAG 
GGGATGCTTA GAGCACACAA AGCTACCATC ATCCAGAAGC ATGTGCGCGG CTGGTTAGCC 
CGTGTCACTT ATAAGAGAAA TTTGAGTGCT ATTGTGTACC TGCAGTGTTG TTACCGCAGG 
ATGATGGCCA AAAGGGAGCT GAAGAAGTTA AAGATAGAGG CACGCTCAGT GGAACACTAC 
AAGAAACTCA ACAATGGGAT GGAAAACAAG ATCATGCAGT TGCAGCGTAA AGTTGATGAG 
CAGAATAAAG ACAACAAATC CTTGCTGGAG AGGCTAACAC ACCTGGAGGT CACGTACAAT 
ACTGAAAAAG ATAAGCTGAG AAGCGACGTC GACAGACTGC GCCATTTTGA AGAGGAGGCC 
AAAAATGCAG CAAACAGGAT GGTCAGCTTG CAGGACGAGC TTGCCAGGCT TAGGAAAGAG 
CTGCAGCAAA CACAGACGGA AAAGAACAAG ATTGAGGAAC GGGCAGAGAA GTACCAGACC 
GAGACTGAAA AGCTGGTTGC TGAGCTGAGA GAACAGAATG CTTTGCTGAA AGCTGAGAAA 
GAGGAACTGA ATCTTTTAAT CCAGGAGCAG GCAAAAAAAA TGACAGAGGA TATGGAAAAG 
AAAATTATAG AAGAAACAAA GCAGTTGGAG CTTGAGCTGA ATGATGAACG ACTGAGGTAC 
CAGAATCTCC TAAACGAGTA CAGCCGCCTG GAGGAGCGAT ATGATGACCT TAAAGATGAA 
ATTAATACCA TGAGTCCTCC AAAGCCAGGA CACAAGCGCA CAGACTCTAC TCATAGCAGC 
AATGAATCAG AATACACTTT TAGTTCAGAG ATCACAGAGT CAGAAGATTA CCCATATCGA 
AATGAGGAGC CTGCTGACAG AAAAACTCCG CTGGACATGT CCCTGTTCCT TAAGCTTCAG 
AAACGGGTAA AGGAACTTGA GCAGGAAAAG CAAGTAATGC AAGATGATTT GGATAAGAAG 
GAGGAGCAGA TAATCCGAGC CAAAATAATG GAGGAGGCAA AGACTCCAAC TAGAGGTACA 
GAACTGGAAT ATGAATCACT CAAGCGTCAA GAACTGGAAT CTGAAAATAA AAAACTTAAG 
AATGAGTTAA ATGAGCTGCG AAGGGCAATC ACTGAGAAAG CCTCCCCAGA TGCTACAGGC 
CCTGGTGGAA CTGTCTATAA AGTTCTCTTG GAGCAGATGA CGTCAGTAAG TGAGGAACTT 
GAAGTACGCA AGGAAGAAGT CCTTATACTG AGGTCCCAGT TGGTGAGCCA AATGGAAGCC 
ATTCCACACA AGGATGACAA GGACACAATG ACCGATTCCA TGGTCCTCTC TGAGGATGTA 
CAGAAGATGA AGGATAAAAG GGAAATTGCT CAGGCCTACA CTGGCATGAA GGAGACAAAT 
AGGGTTTTGG AATCCCAGCT GCAATCACAG AAAAAGGCCT TTGAGAATGA AGTGGAAGCT 
CTCAGAGGTG AGATCCAGGC TCTAAAAGAG GAGAACAACC GGCAGCAGCA ACTTTTAGCC 
CAGAACCTCC AGTTGCCACC TGAAGCACGA ATCGAAGCCA GCTTGCAGCA TGAAATCACC 
AGGCTAACCA ACGAGAACTT GGATCTGATG GAGCAACTTG AAAAGCAAGA TAAGACTGTC 
CGCAAACTGA AAAAGCAGTT GAAAGTCTTT GCCAAGAAAA TTGGAGAGCT GGAAGTTGGT 
CAAATGGAAA ATGTTTCTCC TGGTCAGATA GTGGATGAAC CCATTCGTCC TGTTAATATT 
CCGCGCAAAG AGAAGGACTT CCAAGGAATG CTGGAATATA AGAAAGAGGA CGAACTAAAG 
CTTGTCAAAA ACCTCATTTT AGAACTGAAG CCTCGTGGTG TCGCGGTCAA CCTCATTCCA 
GGACTGCCAG CATATATCTT GTTCATGTGT TTGCGTCATG CTGATTACCT CAATGATGAT 
CAGAAAGTTC GCTCCTTGCT TACATCAACT ATTAATGGTA TCAAGAAAAT CTTAAAGAAA 
AGAGGAGATG ATTTTGAAAC GGTGTCCTTC TGGCTGTCCA ATTCGTGTCG GTTCCTCCAT 
TGCTTGAAGC AGTACAGTGG AGAAGAGGGA TTTATGAAGC ACAACACCAC ACGGCAGAAT 
GAGCACTGTC TGACCAACTT TGACCTTGCG GAATACAGAC AAGTCTTGAG TGATCTGGCT 
ATTCAGATTT ACCAGCAGTT GGTCAGAGTG TTGGAAAATA TCTTGCAGCC AATGATTGTT 
TCAGGAATGC TGGAGCACGA GACTATTCAG GGTGTCTCAG GGGTCAAGCC TACCGGTCTT 
AGGAAGAGAA CTTCCAGCAT AGCAGATGAA GGGACGTACA CATTAGACTC CATTGTGAGG 
CAGCTTAATA CCTTCCATTC CATAATGTGT CAGCATGGGA TGGATCCTGA ACTCATCAAG 
CAGGTGGTCA AGCAAATGTT CTACATCATC GGTGCTGTCA CACTCAACAA CTTGCTCTTG 
CGAAAGGACA TGTGCTCTTG GAGCAAAGGC ATGCAGATAA GGTACAACGT GAGCCAGTTG 
GAAGAATGGC TGCGTGACAA GAACCTGATG AATAGCAGCG CTAAAGAAAC CCTGGAGCCA 
TTAATTCAGG CTGCTCAGTT ACTGCAGGTG AAAAAGAAAA CAGACGAAGA TGCAGAAGCC 
ATCTGCTCCA TGTGCAATGC TCTGACTACT GCCCAGATTG TCAAGGTGTT GAACCTGTAC 
ACTCCAGTAA ATGAGTTTGA AGAGCGGGTG TTGGTATCAT TTATACGGAA TATACAGATG 
CGTTTAAGGG ATAGGAAGGA CTCCCCTCAG CTGCTAATGG ATGCTAAGCA TATCTTCCCT 
GTCACCTTCC CATTCAACCC TTCGTCGCTT GCCCTAGAAA CAATTCAGAT TCCTGGCAGT 
CTTGGCCTTG CCTTCCTCAC ACGTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755051.1
CDS	352..5841
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDDKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_031899191.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899191.1

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGAAGTTTAC ACTGCTAACA GAACTGTTCC AAGAAGAGGA GCAGATCTTG 
AGCCCTACAT CTTCTGCACC ACCCTCAGGC CGTACTCTGC TATCCCGCAC TGGTCTGCGT 
TCCCTCAAGC CCAAGCCTGA ACAGACAAGC AAGGAGCATA AAAAAACTGT AGGACATCAG 
TTCAGAAATT CCCTTCACCT TCTGATGGAG ACTCTCAATG CCACAACTCC ACATTATGTC 
CGTTGCATTA AGCCAAATGA CTTCAAGTAC CCGTTCACAT TTGACTCTAA ACGAGCAGTG 
CAGCAGCTTA GGGCTTGTGG CGTCCTGGAA ACCATCCGAA TCAGTGCTGC TGGATTTCCT 
TCTAGGTGGA CATATCAGGA ATTTTTTAGC AGATATCGTG TTTTAATGAA GCAAAAAGAT 
GTCCTCAGTG ATAGGAAGCA AACCTGCAAA AATGTTCTGG AGAAGCTTAT TTTGGATAAG 
GATAAGTACC AGTTTGGTAA GACAAAGATT TTTTTCCGTG CTGGCCAAGT GGCCTATCTG 
GAGAAGATCC GAGCAGACAA GCTGAGAATG GCCTGTATCA GGATCCAGAA GACAATCCGA 
GGATGGCTTT TGAGGAAGAA ATACTTGCGC ATGAGGAAAG CAGCCATAAC AATCCAGCGC 
TACGTCAGAG GATACCAGGC ACGATGTTAT GCACAGTTTC TGCGTAGAAC ACAGGCCGCG 
ATTATTATCC AGAAATTCCA AAGGATGTAT GTCGTTCGGC AAAAGTACCG TCATATGCAG 
TCCATTACCC TTGCTCTCCA GTCGTATATG CGTGGTTATG CAGCCAGGAA GAGATTTCAG 
GGGATGCTTA GAGCACACAA AGCTACCATC ATCCAGAAGC ATGTGCGCGG CTGGTTAGCC 
CGTGTCACTT ATAAGAGAAA TTTGAGTGCT ATTGTGTACC TGCAGTGTTG TTACCGCAGG 
ATGATGGCCA AAAGGGAGCT GAAGAAGTTA AAGATAGAGG CACGCTCAGT GGAACACTAC 
AAGAAACTCA ACAATGGGAT GGAAAACAAG ATCATGCAGT TGCAGCGTAA AGTTGATGAG 
CAGAATAAAG ACAACAAATC CTTGCTGGAG AGGCTAACAC ACCTGGAGGT CACGTACAAT 
ACTGAAAAAG ATAAGCTGAG AAGCGACGTC GACAGACTGC GCCATTTTGA AGAGGAGGCC 
AAAAATGCAG CAAACAGGAT GGTCAGCTTG CAGGACGAGC TTGCCAGGCT TAGGAAAGAG 
CTGCAGCAAA CACAGACGGA AAAGAACAAG ATTGAGGAAC GGGCAGAGAA GTACCAGACC 
GAGACTGAAA AGCTGGTTGC TGAGCTGAGA GAACAGAATG CTTTGCTGAA AGCTGAGAAA 
GAGGAACTGA ATCTTTTAAT CCAGGAGCAG GCAAAAAAAA TGACAGAGGA TATGGAAAAG 
AAAATTATAG AAGAAACAAA GCAGTTGGAG CTTGAGCTGA ATGATGAACG ACTGAGGTAC 
CAGAATCTCC TAAACGAGTA CAGCCGCCTG GAGGAGCGAT ATGATGACCT TAAAGATGAA 
ATTAATACCA TGAGTCCTCC AAAGCCAGGA CACAAGCGCA CAGACTCTAC TCATAGCAGC 
AATGAATCAG AATACACTTT TAGTTCAGAG ATCACAGAGT CAGAAGATTA CCCATATCGA 
AATGAGGAGC CTGCTGACAG AAAAACTCCG CTGGACATGT CCCTGTTCCT TAAGCTTCAG 
AAACGGGTAA AGGAACTTGA GCAGGAAAAG CAAGTAATGC AAGATGATTT GGATAAGAAG 
GAGGAGCAGA TAATCCGAGC CAAAATAATG GAGGAGGCAA AGACTCCAAC TAGAGGTACA 
GAACTGGAAT ATGAATCACT CAAGCGTCAA GAACTGGAAT CTGAAAATAA AAAACTTAAG 
AATGAGTTAA ATGAGCTGCG AAGGGCAATC ACTGAGAAAG CCTCCCCAGA TGCTACAGGC 
CCTGGTGGAA CTGTCTATAA AGTTCTCTTG GAGCAGATGA CGTCAGTAAG TGAGGAACTT 
GAAGTACGCA AGGAAGAAGT CCTTATACTG AGGTCCCAGT TGGTGAGCCA AATGGAAGCC 
ATTCCACACA AGGATGACAA GGACACAATG ACCGATTCCA TGGTCCTCTC TGAGGATGTA 
CAGAAGATGA AGGATAAAAG GGAAATTGCT CAGGCCTACA CTGGCATGAA GGAGACAAAT 
AGGGTTTTGG AATCCCAGCT GCAATCACAG AAAAAGGCCT TTGAGAATGA AGTGGAAGCT 
CTCAGAGGTG AGATCCAGGC TCTAAAAGAG GAGAACAACC GGCAGCAGCA ACTTTTAGCC 
CAGAACCTCC AGTTGCCACC TGAAGCACGA ATCGAAGCCA GCTTGCAGCA TGAAATCACC 
AGGCTAACCA ACGAGAACTT GGATCTGATG GAGCAACTTG AAAAGCAAGA TAAGACTGTC 
CGCAAACTGA AAAAGCAGTT GAAAGTCTTT GCCAAGAAAA TTGGAGAGCT GGAAGTTGGT 
CAAATGGAAA ATGTTTCTCC TGGTCAGATA GTGGATGAAC CCATTCGTCC TGTTAATATT 
CCGCGCAAAG AGAAGGACTT CCAAGGAATG CTGGAATATA AGAAAGAGGA CGAACTAAAG 
CTTGTCAAAA ACCTCATTTT AGAACTGAAG CCTCGTGGTG TCGCGGTCAA CCTCATTCCA 
GGACTGCCAG CATATATCTT GTTCATGTGT TTGCGTCATG CTGATTACCT CAATGATGAT 
CAGAAAGTTC GCTCCTTGCT TACATCAACT ATTAATGGTA TCAAGAAAAT CTTAAAGAAA 
AGAGGAGATG ATTTTGAAAC GGTGTCCTTC TGGCTGTCCA ATTCGTGTCG GTTCCTCCAT 
TGCTTGAAGC AGTACAGTGG AGAAGAGGGA TTTATGAAGC ACAACACCAC ACGGCAGAAT 
GAGCACTGTC TGACCAACTT TGACCTTGCG GAATACAGAC AAGTCTTGAG TGATCTGGCT 
ATTCAGATTT ACCAGCAGTT GGTCAGAGTG TTGGAAAATA TCTTGCAGCC AATGATTGTT 
TCAGGAATGC TGGAGCACGA GACTATTCAG GGTGTCTCAG GGGTCAAGCC TACCGGTCTT 
AGGAAGAGAA CTTCCAGCAT AGCAGATGAA GGGACGTACA CATTAGACTC CATTGTGAGG 
CAGCTTAATA CCTTCCATTC CATAATGTGT CAGCATGGGA TGGATCCTGA ACTCATCAAG 
CAGGTGGTCA AGCAAATGTT CTACATCATC GGTGCTGTCA CACTCAACAA CTTGCTCTTG 
CGAAAGGACA TGTGCTCTTG GAGCAAAGGC ATGCAGATAA GGTACAACGT GAGCCAGTTG 
GAAGAATGGC TGCGTGACAA GAACCTGATG AATAGCAGCG CTAAAGAAAC CCTGGAGCCA 
TTAATTCAGG CTGCTCAGTT ACTGCAGGTG AAAAAGAAAA CAGACGAAGA TGCAGAAGCC 
ATCTGCTCCA TGTGCAATGC TCTGACTACT GCCCAGATTG TCAAGGTGTT GAACCTGTAC 
ACTCCAGTAA ATGAGTTTGA AGAGCGGGTG TTGGTATCAT TTATACGGAA TATACAGATG 
CGTTTAAGGG ATAGGAAGGA CTCCCCTCAG CTGCTAATGG ATGCTAAGCA TATCTTCCCT 
GTCACCTTCC CATTCAACCC TTCGTCGCTT GCCCTAGAAA CAATTCAGAT TCCTGGCAGT 
CTTGGCCTTG CCTTCCTCAC ACGTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_002936347.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936393.3 Xenopus tropicalis myosin VA (myo5a), transcript variant X14, mRNA.

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CloneID	OXa55102
Clone ID Related Accession (Same CDS sequence)	XM_002936347.5
Accession Version	XM_002936347.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936347.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACA GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAAA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ATGACAAGGA CACAATGACC GATTCCATGG TCCTCTCTGA GGATGTACAG 
AAGATGAAGG ATAAAAGGGA AATTGCTCAG GCCTACACTG GCATGAAGGA GACAAATAGG 
GTTTTGGAAT CCCAGCTGCA ATCACAGAAA AAGGCCTTTG AGAATGAAGT GGAAGCTCTC 
AGAGGTGAGA TCCAGGCTCT AAAAGAGGAG AACAACCGGC AGCAGCAACT TTTAGCCCAG 
AACCTCCAGT TGCCACCTGA AGCACGAATC GAAGCCAGCT TGCAGCATGA AATCACCAGG 
CTAACCAACG AGAACTTGGA TCTGATGGAG CAACTTGAAA AGCAAGATAA GACTGTCCGC 
AAACTGAAAA AGCAGTTGAA AGTCTTTGCC AAGAAAATTG GAGAGCTGGA AGTTGGTCAA 
ATGGAAAATG TTTCTCCTGG TCAGATAGTG GATGAACCCA TTCGTCCTGT TAATATTCCG 
CGCAAAGAGA AGGACTTCCA AGGAATGCTG GAATATAAGA AAGAGGACGA ACTAAAGCTT 
GTCAAAAACC TCATTTTAGA ACTGAAGCCT CGTGGTGTCG CGGTCAACCT CATTCCAGGA 
CTGCCAGCAT ATATCTTGTT CATGTGTTTG CGTCATGCTG ATTACCTCAA TGATGATCAG 
AAAGTTCGCT CCTTGCTTAC ATCAACTATT AATGGTATCA AGAAAATCTT AAAGAAAAGA 
GGAGATGATT TTGAAACGGT GTCCTTCTGG CTGTCCAATT CGTGTCGGTT CCTCCATTGC 
TTGAAGCAGT ACAGTGGAGA AGAGGGATTT ATGAAGCACA ACACCACACG GCAGAATGAG 
CACTGTCTGA CCAACTTTGA CCTTGCGGAA TACAGACAAG TCTTGAGTGA TCTGGCTATT 
CAGATTTACC AGCAGTTGGT CAGAGTGTTG GAAAATATCT TGCAGCCAAT GATTGTTTCA 
GGAATGCTGG AGCACGAGAC TATTCAGGGT GTCTCAGGGG TCAAGCCTAC CGGTCTTAGG 
AAGAGAACTT CCAGCATAGC AGATGAAGGG ACGTACACAT TAGACTCCAT TGTGAGGCAG 
CTTAATACCT TCCATTCCAT AATGTGTCAG CATGGGATGG ATCCTGAACT CATCAAGCAG 
GTGGTCAAGC AAATGTTCTA CATCATCGGT GCTGTCACAC TCAACAACTT GCTCTTGCGA 
AAGGACATGT GCTCTTGGAG CAAAGGCATG CAGATAAGGT ACAACGTGAG CCAGTTGGAA 
GAATGGCTGC GTGACAAGAA CCTGATGAAT AGCAGCGCTA AAGAAACCCT GGAGCCATTA 
ATTCAGGCTG CTCAGTTACT GCAGGTGAAA AAGAAAACAG ACGAAGATGC AGAAGCCATC 
TGCTCCATGT GCAATGCTCT GACTACTGCC CAGATTGTCA AGGTGTTGAA CCTGTACACT 
CCAGTAAATG AGTTTGAAGA GCGGGTGTTG GTATCATTTA TACGGAATAT ACAGATGCGT 
TTAAGGGATA GGAAGGACTC CCCTCAGCTG CTAATGGATG CTAAGCATAT CTTCCCTGTC 
ACCTTCCCAT TCAACCCTTC GTCGCTTGCC CTAGAAACAA TTCAGATTCC TGGCAGTCTT 
GGCCTTGCCT TCCTCACACG TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936393.3
CDS	351..5837
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTQAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDDKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_002936347.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936347.5

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTCATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC GGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGAA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACA GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAAA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ATGACAAGGA CACAATGACC GATTCCATGG TCCTCTCTGA GGATGTACAG 
AAGATGAAGG ATAAAAGGGA AATTGCTCAG GCCTACACTG GCATGAAGGA GACAAATAGG 
GTTTTGGAAT CCCAGCTGCA ATCACAGAAA AAGGCCTTTG AGAATGAAGT GGAAGCTCTC 
AGAGGTGAGA TCCAGGCTCT AAAAGAGGAG AACAACCGGC AGCAGCAACT TTTAGCCCAG 
AACCTCCAGT TGCCACCTGA AGCACGAATC GAAGCCAGCT TGCAGCATGA AATCACCAGG 
CTAACCAACG AGAACTTGGA TCTGATGGAG CAACTTGAAA AGCAAGATAA GACTGTCCGC 
AAACTGAAAA AGCAGTTGAA AGTCTTTGCC AAGAAAATTG GAGAGCTGGA AGTTGGTCAA 
ATGGAAAATG TTTCTCCTGG TCAGATAGTG GATGAACCCA TTCGTCCTGT TAATATTCCG 
CGCAAAGAGA AGGACTTCCA AGGAATGCTG GAATATAAGA AAGAGGACGA ACTAAAGCTT 
GTCAAAAACC TCATTTTAGA ACTGAAGCCT CGTGGTGTCG CGGTCAACCT CATTCCAGGA 
CTGCCAGCAT ATATCTTGTT CATGTGTTTG CGTCATGCTG ATTACCTCAA TGATGATCAG 
AAAGTTCGCT CCTTGCTTAC ATCAACTATT AATGGTATCA AGAAAATCTT AAAGAAAAGA 
GGAGATGATT TTGAAACGGT GTCCTTCTGG CTGTCCAATT CGTGTCGGTT CCTCCATTGC 
TTGAAGCAGT ACAGTGGAGA AGAGGGATTT ATGAAGCACA ACACCACACG GCAGAATGAG 
CACTGTCTGA CCAACTTTGA CCTTGCGGAA TACAGACAAG TCTTGAGTGA TCTGGCTATT 
CAGATTTACC AGCAGTTGGT CAGAGTGTTG GAAAATATCT TGCAGCCAAT GATTGTTTCA 
GGAATGCTGG AGCACGAGAC TATTCAGGGT GTCTCAGGGG TCAAGCCTAC CGGTCTTAGG 
AAGAGAACTT CCAGCATAGC AGATGAAGGG ACGTACACAT TAGACTCCAT TGTGAGGCAG 
CTTAATACCT TCCATTCCAT AATGTGTCAG CATGGGATGG ATCCTGAACT CATCAAGCAG 
GTGGTCAAGC AAATGTTCTA CATCATCGGT GCTGTCACAC TCAACAACTT GCTCTTGCGA 
AAGGACATGT GCTCTTGGAG CAAAGGCATG CAGATAAGGT ACAACGTGAG CCAGTTGGAA 
GAATGGCTGC GTGACAAGAA CCTGATGAAT AGCAGCGCTA AAGAAACCCT GGAGCCATTA 
ATTCAGGCTG CTCAGTTACT GCAGGTGAAA AAGAAAACAG ACGAAGATGC AGAAGCCATC 
TGCTCCATGT GCAATGCTCT GACTACTGCC CAGATTGTCA AGGTGTTGAA CCTGTACACT 
CCAGTAAATG AGTTTGAAGA GCGGGTGTTG GTATCATTTA TACGGAATAT ACAGATGCGT 
TTAAGGGATA GGAAGGACTC CCCTCAGCTG CTAATGGATG CTAAGCATAT CTTCCCTGTC 
ACCTTCCCAT TCAACCCTTC GTCGCTTGCC CTAGAAACAA TTCAGATTCC TGGCAGTCTT 
GGCCTTGCCT TCCTCACACG TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_004912695.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912752.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X1, mRNA.

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CloneID	OXa07363
Clone ID Related Accession (Same CDS sequence)	XM_004912695.3
Accession Version	XM_004912695.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912695.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTTATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC AGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGGA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACG GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAGA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ATGACAAGGA CACAATGACC GATTCCATGG TCCTCTCTGA GGATGTACAG 
AAGATGAAGG ATAAAAGGGA AATTGCTCAG GCCTACACTG GCATGAAGGA GACAAATAGG 
GTTTTGGAAT CCCAGCTGCA ATCACAGAAA AAGGCCTTTG AGAATGAAGT GGAAGCTCTC 
AGAGGTGAGA TCCAGGCTCT AAAAGAGGAG AACAACCGGC AGCAGCAACT TTTAGCCCAG 
AACCTCCAGT TGCCACCTGA AGCACGAATC GAAGCCAGCT TGCAGCATGA AATCACCAGG 
CTAACCAACG AGAACTTGTT TTTTGAGGAG TTATATGCAG ATGAACCCAT GAAGTATCAA 
TCGTACCGGA TCTCCCTGTA CAGACGGATC ATTGATCTGA TGGAGCAACT TGAAAAGCAA 
GATAAGACTG TCCGCAAACT GAAAAAGCAG TTGAAAGTCT TTGCCAAGAA AATTGGAGAG 
CTGGAAGTTG GTCAAATGGA AAATGTTTCT CCTGGTCAGA TAGTGGATGA ACCCATTCGT 
CCTGTTAATA TTCCGCGCAA AGAGAAGGAC TTCCAAGGAA TGCTGGAATA TAAGAAAGAG 
GATGAACTAA AGCTTGTCAA AAACCTCATT TTAGAACTGA AGCCTCGTGG TGTCGCGGTC 
AACCTCATTC CAGGACTGCC AGCATATATC TTGTTCATGT GTTTGCGTCA TGCTGATTAC 
CTCAATGATG ATCAGAAAGT TCGCTCCTTG CTTACATCAA CTATTAATGG TATCAAGAAA 
ATCTTAAAGA AAAGAGGAGA TGATTTTGAA ACGGTGTCCT TCTGGCTGTC CAATTCGTGT 
CGGTTCCTCC ATTGCTTGAA GCAGTACAGT GGAGAAGAGG GATTTATGAA GCACAACACC 
ACACGGCAGA ATGAGCACTG TCTGACCAAC TTTGACCTTG CGGAATACAG ACAAGTCTTG 
AGTGATCTGG CTATTCAGAT TTACCAGCAG TTGGTCAGAG TGTTGGAAAA TATCTTGCAG 
CCAATGATTG TTTCAGGAAT GCTGGAGCAC GAGACTATTC AGGGTGTCTC AGGGGTCAAG 
CCTACCGGTC TTAGGAAGAG AACTTCCAGC ATAGCAGATG AAGGGACGTA CACATTAGAC 
TCCATTGTGA GGCAGCTTAA TACCTTCCAT TCCATAATGT GTCAGCATGG GATGGATCCT 
GAACTCATCA AGCAGGTGGT CAAGCAAATG TTCTACATCA TCGGTGCTGT CACACTCAAC 
AACTTGCTCT TGCGAAAGGA CATGTGCTCT TGGAGCAAAG GCATGCAGAT AAGGTACAAC 
GTGAGCCAGT TGGAAGAATG GCTGCGTGAC AAGAACCTGA TGAATAGCAG CGCTAAAGAA 
ACCCTGGAGC CATTAATTCA GGCTGCTCAG TTACTGCAGG TGAAAAAGAA AACAGATGAA 
GATGCAGAAG CCATCTGCTC CATGTGCAAT GCTCTGACTA CTGCCCAGAT TGTCAAGGTG 
TTGAACCTGT ACACTCCAGT AAATGAGTTT GAAGAGCGGG TGTTGGTATC ATTTATACGG 
AATATACAGA TGCGTTTAAG GGATAGGAAG GACTCCCCTC AGCTGCTAAT GGATGCTAAG 
CATATCTTCC CTGTCACCTT CCCATTCAAC CCTTCGTCGC TTGCCCTAGA AACAATTCAG 
ATTCCTGGCA GTCTTGGCCT TGCCTTCCTC ACACGTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912752.1
CDS	1035..6596
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTRAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDDKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLFFEELYADEPMKYQSYRISLYRRIIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_004912695.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912695.3

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTTATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC AGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGGA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACG GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAGA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ATGACAAGGA CACAATGACC GATTCCATGG TCCTCTCTGA GGATGTACAG 
AAGATGAAGG ATAAAAGGGA AATTGCTCAG GCCTACACTG GCATGAAGGA GACAAATAGG 
GTTTTGGAAT CCCAGCTGCA ATCACAGAAA AAGGCCTTTG AGAATGAAGT GGAAGCTCTC 
AGAGGTGAGA TCCAGGCTCT AAAAGAGGAG AACAACCGGC AGCAGCAACT TTTAGCCCAG 
AACCTCCAGT TGCCACCTGA AGCACGAATC GAAGCCAGCT TGCAGCATGA AATCACCAGG 
CTAACCAACG AGAACTTGTT TTTTGAGGAG TTATATGCAG ATGAACCCAT GAAGTATCAA 
TCGTACCGGA TCTCCCTGTA CAGACGGATC ATTGATCTGA TGGAGCAACT TGAAAAGCAA 
GATAAGACTG TCCGCAAACT GAAAAAGCAG TTGAAAGTCT TTGCCAAGAA AATTGGAGAG 
CTGGAAGTTG GTCAAATGGA AAATGTTTCT CCTGGTCAGA TAGTGGATGA ACCCATTCGT 
CCTGTTAATA TTCCGCGCAA AGAGAAGGAC TTCCAAGGAA TGCTGGAATA TAAGAAAGAG 
GATGAACTAA AGCTTGTCAA AAACCTCATT TTAGAACTGA AGCCTCGTGG TGTCGCGGTC 
AACCTCATTC CAGGACTGCC AGCATATATC TTGTTCATGT GTTTGCGTCA TGCTGATTAC 
CTCAATGATG ATCAGAAAGT TCGCTCCTTG CTTACATCAA CTATTAATGG TATCAAGAAA 
ATCTTAAAGA AAAGAGGAGA TGATTTTGAA ACGGTGTCCT TCTGGCTGTC CAATTCGTGT 
CGGTTCCTCC ATTGCTTGAA GCAGTACAGT GGAGAAGAGG GATTTATGAA GCACAACACC 
ACACGGCAGA ATGAGCACTG TCTGACCAAC TTTGACCTTG CGGAATACAG ACAAGTCTTG 
AGTGATCTGG CTATTCAGAT TTACCAGCAG TTGGTCAGAG TGTTGGAAAA TATCTTGCAG 
CCAATGATTG TTTCAGGAAT GCTGGAGCAC GAGACTATTC AGGGTGTCTC AGGGGTCAAG 
CCTACCGGTC TTAGGAAGAG AACTTCCAGC ATAGCAGATG AAGGGACGTA CACATTAGAC 
TCCATTGTGA GGCAGCTTAA TACCTTCCAT TCCATAATGT GTCAGCATGG GATGGATCCT 
GAACTCATCA AGCAGGTGGT CAAGCAAATG TTCTACATCA TCGGTGCTGT CACACTCAAC 
AACTTGCTCT TGCGAAAGGA CATGTGCTCT TGGAGCAAAG GCATGCAGAT AAGGTACAAC 
GTGAGCCAGT TGGAAGAATG GCTGCGTGAC AAGAACCTGA TGAATAGCAG CGCTAAAGAA 
ACCCTGGAGC CATTAATTCA GGCTGCTCAG TTACTGCAGG TGAAAAAGAA AACAGATGAA 
GATGCAGAAG CCATCTGCTC CATGTGCAAT GCTCTGACTA CTGCCCAGAT TGTCAAGGTG 
TTGAACCTGT ACACTCCAGT AAATGAGTTT GAAGAGCGGG TGTTGGTATC ATTTATACGG 
AATATACAGA TGCGTTTAAG GGATAGGAAG GACTCCCCTC AGCTGCTAAT GGATGCTAAG 
CATATCTTCC CTGTCACCTT CCCATTCAAC CCTTCGTCGC TTGCCCTAGA AACAATTCAG 
ATTCCTGGCA GTCTTGGCCT TGCCTTCCTC ACACGTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_004912696.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912753.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X2, mRNA.

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CloneID	OXa07364
Clone ID Related Accession (Same CDS sequence)	XM_004912696.3
Accession Version	XM_004912696.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912696.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTTATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC AGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGGA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACG GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAGA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ACACAATGAC CGATTCCATG GTCCTCTCTG AGGATGTACA GAAGATGAAG 
GATAAAAGGG AAATTGCTCA GGCCTACACT GGCATGAAGG AGACAAATAG GGTTTTGGAA 
TCCCAGCTGC AATCACAGAA AAAGGCCTTT GAGAATGAAG TGGAAGCTCT CAGAGGTGAG 
ATCCAGGCTC TAAAAGAGGA GAACAACCGG CAGCAGCAAC TTTTAGCCCA GAACCTCCAG 
TTGCCACCTG AAGCACGAAT CGAAGCCAGC TTGCAGCATG AAATCACCAG GCTAACCAAC 
GAGAACTTGT TTTTTGAGGA GTTATATGCA GATGAACCCA TGAAGTATCA ATCGTACCGG 
ATCTCCCTGT ACAGACGGAT CATTGATCTG ATGGAGCAAC TTGAAAAGCA AGATAAGACT 
GTCCGCAAAC TGAAAAAGCA GTTGAAAGTC TTTGCCAAGA AAATTGGAGA GCTGGAAGTT 
GGTCAAATGG AAAATGTTTC TCCTGGTCAG ATAGTGGATG AACCCATTCG TCCTGTTAAT 
ATTCCGCGCA AAGAGAAGGA CTTCCAAGGA ATGCTGGAAT ATAAGAAAGA GGATGAACTA 
AAGCTTGTCA AAAACCTCAT TTTAGAACTG AAGCCTCGTG GTGTCGCGGT CAACCTCATT 
CCAGGACTGC CAGCATATAT CTTGTTCATG TGTTTGCGTC ATGCTGATTA CCTCAATGAT 
GATCAGAAAG TTCGCTCCTT GCTTACATCA ACTATTAATG GTATCAAGAA AATCTTAAAG 
AAAAGAGGAG ATGATTTTGA AACGGTGTCC TTCTGGCTGT CCAATTCGTG TCGGTTCCTC 
CATTGCTTGA AGCAGTACAG TGGAGAAGAG GGATTTATGA AGCACAACAC CACACGGCAG 
AATGAGCACT GTCTGACCAA CTTTGACCTT GCGGAATACA GACAAGTCTT GAGTGATCTG 
GCTATTCAGA TTTACCAGCA GTTGGTCAGA GTGTTGGAAA ATATCTTGCA GCCAATGATT 
GTTTCAGGAA TGCTGGAGCA CGAGACTATT CAGGGTGTCT CAGGGGTCAA GCCTACCGGT 
CTTAGGAAGA GAACTTCCAG CATAGCAGAT GAAGGGACGT ACACATTAGA CTCCATTGTG 
AGGCAGCTTA ATACCTTCCA TTCCATAATG TGTCAGCATG GGATGGATCC TGAACTCATC 
AAGCAGGTGG TCAAGCAAAT GTTCTACATC ATCGGTGCTG TCACACTCAA CAACTTGCTC 
TTGCGAAAGG ACATGTGCTC TTGGAGCAAA GGCATGCAGA TAAGGTACAA CGTGAGCCAG 
TTGGAAGAAT GGCTGCGTGA CAAGAACCTG ATGAATAGCA GCGCTAAAGA AACCCTGGAG 
CCATTAATTC AGGCTGCTCA GTTACTGCAG GTGAAAAAGA AAACAGATGA AGATGCAGAA 
GCCATCTGCT CCATGTGCAA TGCTCTGACT ACTGCCCAGA TTGTCAAGGT GTTGAACCTG 
TACACTCCAG TAAATGAGTT TGAAGAGCGG GTGTTGGTAT CATTTATACG GAATATACAG 
ATGCGTTTAA GGGATAGGAA GGACTCCCCT CAGCTGCTAA TGGATGCTAA GCATATCTTC 
CCTGTCACCT TCCCATTCAA CCCTTCGTCG CTTGCCCTAG AAACAATTCA GATTCCTGGC 
AGTCTTGGCC TTGCCTTCCT CACACGTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912753.1
CDS	1034..6586
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTRAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLFFEELYADEPMKYQSYRISLYRRIIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_004912696.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912696.3

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTTATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC AGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGGA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACG GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAGA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ACACAATGAC CGATTCCATG GTCCTCTCTG AGGATGTACA GAAGATGAAG 
GATAAAAGGG AAATTGCTCA GGCCTACACT GGCATGAAGG AGACAAATAG GGTTTTGGAA 
TCCCAGCTGC AATCACAGAA AAAGGCCTTT GAGAATGAAG TGGAAGCTCT CAGAGGTGAG 
ATCCAGGCTC TAAAAGAGGA GAACAACCGG CAGCAGCAAC TTTTAGCCCA GAACCTCCAG 
TTGCCACCTG AAGCACGAAT CGAAGCCAGC TTGCAGCATG AAATCACCAG GCTAACCAAC 
GAGAACTTGT TTTTTGAGGA GTTATATGCA GATGAACCCA TGAAGTATCA ATCGTACCGG 
ATCTCCCTGT ACAGACGGAT CATTGATCTG ATGGAGCAAC TTGAAAAGCA AGATAAGACT 
GTCCGCAAAC TGAAAAAGCA GTTGAAAGTC TTTGCCAAGA AAATTGGAGA GCTGGAAGTT 
GGTCAAATGG AAAATGTTTC TCCTGGTCAG ATAGTGGATG AACCCATTCG TCCTGTTAAT 
ATTCCGCGCA AAGAGAAGGA CTTCCAAGGA ATGCTGGAAT ATAAGAAAGA GGATGAACTA 
AAGCTTGTCA AAAACCTCAT TTTAGAACTG AAGCCTCGTG GTGTCGCGGT CAACCTCATT 
CCAGGACTGC CAGCATATAT CTTGTTCATG TGTTTGCGTC ATGCTGATTA CCTCAATGAT 
GATCAGAAAG TTCGCTCCTT GCTTACATCA ACTATTAATG GTATCAAGAA AATCTTAAAG 
AAAAGAGGAG ATGATTTTGA AACGGTGTCC TTCTGGCTGT CCAATTCGTG TCGGTTCCTC 
CATTGCTTGA AGCAGTACAG TGGAGAAGAG GGATTTATGA AGCACAACAC CACACGGCAG 
AATGAGCACT GTCTGACCAA CTTTGACCTT GCGGAATACA GACAAGTCTT GAGTGATCTG 
GCTATTCAGA TTTACCAGCA GTTGGTCAGA GTGTTGGAAA ATATCTTGCA GCCAATGATT 
GTTTCAGGAA TGCTGGAGCA CGAGACTATT CAGGGTGTCT CAGGGGTCAA GCCTACCGGT 
CTTAGGAAGA GAACTTCCAG CATAGCAGAT GAAGGGACGT ACACATTAGA CTCCATTGTG 
AGGCAGCTTA ATACCTTCCA TTCCATAATG TGTCAGCATG GGATGGATCC TGAACTCATC 
AAGCAGGTGG TCAAGCAAAT GTTCTACATC ATCGGTGCTG TCACACTCAA CAACTTGCTC 
TTGCGAAAGG ACATGTGCTC TTGGAGCAAA GGCATGCAGA TAAGGTACAA CGTGAGCCAG 
TTGGAAGAAT GGCTGCGTGA CAAGAACCTG ATGAATAGCA GCGCTAAAGA AACCCTGGAG 
CCATTAATTC AGGCTGCTCA GTTACTGCAG GTGAAAAAGA AAACAGATGA AGATGCAGAA 
GCCATCTGCT CCATGTGCAA TGCTCTGACT ACTGCCCAGA TTGTCAAGGT GTTGAACCTG 
TACACTCCAG TAAATGAGTT TGAAGAGCGG GTGTTGGTAT CATTTATACG GAATATACAG 
ATGCGTTTAA GGGATAGGAA GGACTCCCCT CAGCTGCTAA TGGATGCTAA GCATATCTTC 
CCTGTCACCT TCCCATTCAA CCCTTCGTCG CTTGCCCTAG AAACAATTCA GATTCCTGGC 
AGTCTTGGCC TTGCCTTCCT CACACGTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_002936347.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936393.2 Xenopus tropicalis myosin VA (myo5a), transcript variant X3, mRNA.

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CloneID	OXa07365
Clone ID Related Accession (Same CDS sequence)	XM_002936347.4
Accession Version	XM_002936347.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936347.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTTATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC AGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGGA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACG GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAGA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ATGACAAGGA CACAATGACC GATTCCATGG TCCTCTCTGA GGATGTACAG 
AAGATGAAGG ATAAAAGGGA AATTGCTCAG GCCTACACTG GCATGAAGGA GACAAATAGG 
GTTTTGGAAT CCCAGCTGCA ATCACAGAAA AAGGCCTTTG AGAATGAAGT GGAAGCTCTC 
AGAGGTGAGA TCCAGGCTCT AAAAGAGGAG AACAACCGGC AGCAGCAACT TTTAGCCCAG 
AACCTCCAGT TGCCACCTGA AGCACGAATC GAAGCCAGCT TGCAGCATGA AATCACCAGG 
CTAACCAACG AGAACTTGGA TCTGATGGAG CAACTTGAAA AGCAAGATAA GACTGTCCGC 
AAACTGAAAA AGCAGTTGAA AGTCTTTGCC AAGAAAATTG GAGAGCTGGA AGTTGGTCAA 
ATGGAAAATG TTTCTCCTGG TCAGATAGTG GATGAACCCA TTCGTCCTGT TAATATTCCG 
CGCAAAGAGA AGGACTTCCA AGGAATGCTG GAATATAAGA AAGAGGATGA ACTAAAGCTT 
GTCAAAAACC TCATTTTAGA ACTGAAGCCT CGTGGTGTCG CGGTCAACCT CATTCCAGGA 
CTGCCAGCAT ATATCTTGTT CATGTGTTTG CGTCATGCTG ATTACCTCAA TGATGATCAG 
AAAGTTCGCT CCTTGCTTAC ATCAACTATT AATGGTATCA AGAAAATCTT AAAGAAAAGA 
GGAGATGATT TTGAAACGGT GTCCTTCTGG CTGTCCAATT CGTGTCGGTT CCTCCATTGC 
TTGAAGCAGT ACAGTGGAGA AGAGGGATTT ATGAAGCACA ACACCACACG GCAGAATGAG 
CACTGTCTGA CCAACTTTGA CCTTGCGGAA TACAGACAAG TCTTGAGTGA TCTGGCTATT 
CAGATTTACC AGCAGTTGGT CAGAGTGTTG GAAAATATCT TGCAGCCAAT GATTGTTTCA 
GGAATGCTGG AGCACGAGAC TATTCAGGGT GTCTCAGGGG TCAAGCCTAC CGGTCTTAGG 
AAGAGAACTT CCAGCATAGC AGATGAAGGG ACGTACACAT TAGACTCCAT TGTGAGGCAG 
CTTAATACCT TCCATTCCAT AATGTGTCAG CATGGGATGG ATCCTGAACT CATCAAGCAG 
GTGGTCAAGC AAATGTTCTA CATCATCGGT GCTGTCACAC TCAACAACTT GCTCTTGCGA 
AAGGACATGT GCTCTTGGAG CAAAGGCATG CAGATAAGGT ACAACGTGAG CCAGTTGGAA 
GAATGGCTGC GTGACAAGAA CCTGATGAAT AGCAGCGCTA AAGAAACCCT GGAGCCATTA 
ATTCAGGCTG CTCAGTTACT GCAGGTGAAA AAGAAAACAG ATGAAGATGC AGAAGCCATC 
TGCTCCATGT GCAATGCTCT GACTACTGCC CAGATTGTCA AGGTGTTGAA CCTGTACACT 
CCAGTAAATG AGTTTGAAGA GCGGGTGTTG GTATCATTTA TACGGAATAT ACAGATGCGT 
TTAAGGGATA GGAAGGACTC CCCTCAGCTG CTAATGGATG CTAAGCATAT CTTCCCTGTC 
ACCTTCCCAT TCAACCCTTC GTCGCTTGCC CTAGAAACAA TTCAGATTCC TGGCAGTCTT 
GGCCTTGCCT TCCTCACACG TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936393.2
CDS	1031..6517
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTRAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDDKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_002936347.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936347.4

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTTATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC AGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGGA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACG GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAGA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ATGACAAGGA CACAATGACC GATTCCATGG TCCTCTCTGA GGATGTACAG 
AAGATGAAGG ATAAAAGGGA AATTGCTCAG GCCTACACTG GCATGAAGGA GACAAATAGG 
GTTTTGGAAT CCCAGCTGCA ATCACAGAAA AAGGCCTTTG AGAATGAAGT GGAAGCTCTC 
AGAGGTGAGA TCCAGGCTCT AAAAGAGGAG AACAACCGGC AGCAGCAACT TTTAGCCCAG 
AACCTCCAGT TGCCACCTGA AGCACGAATC GAAGCCAGCT TGCAGCATGA AATCACCAGG 
CTAACCAACG AGAACTTGGA TCTGATGGAG CAACTTGAAA AGCAAGATAA GACTGTCCGC 
AAACTGAAAA AGCAGTTGAA AGTCTTTGCC AAGAAAATTG GAGAGCTGGA AGTTGGTCAA 
ATGGAAAATG TTTCTCCTGG TCAGATAGTG GATGAACCCA TTCGTCCTGT TAATATTCCG 
CGCAAAGAGA AGGACTTCCA AGGAATGCTG GAATATAAGA AAGAGGATGA ACTAAAGCTT 
GTCAAAAACC TCATTTTAGA ACTGAAGCCT CGTGGTGTCG CGGTCAACCT CATTCCAGGA 
CTGCCAGCAT ATATCTTGTT CATGTGTTTG CGTCATGCTG ATTACCTCAA TGATGATCAG 
AAAGTTCGCT CCTTGCTTAC ATCAACTATT AATGGTATCA AGAAAATCTT AAAGAAAAGA 
GGAGATGATT TTGAAACGGT GTCCTTCTGG CTGTCCAATT CGTGTCGGTT CCTCCATTGC 
TTGAAGCAGT ACAGTGGAGA AGAGGGATTT ATGAAGCACA ACACCACACG GCAGAATGAG 
CACTGTCTGA CCAACTTTGA CCTTGCGGAA TACAGACAAG TCTTGAGTGA TCTGGCTATT 
CAGATTTACC AGCAGTTGGT CAGAGTGTTG GAAAATATCT TGCAGCCAAT GATTGTTTCA 
GGAATGCTGG AGCACGAGAC TATTCAGGGT GTCTCAGGGG TCAAGCCTAC CGGTCTTAGG 
AAGAGAACTT CCAGCATAGC AGATGAAGGG ACGTACACAT TAGACTCCAT TGTGAGGCAG 
CTTAATACCT TCCATTCCAT AATGTGTCAG CATGGGATGG ATCCTGAACT CATCAAGCAG 
GTGGTCAAGC AAATGTTCTA CATCATCGGT GCTGTCACAC TCAACAACTT GCTCTTGCGA 
AAGGACATGT GCTCTTGGAG CAAAGGCATG CAGATAAGGT ACAACGTGAG CCAGTTGGAA 
GAATGGCTGC GTGACAAGAA CCTGATGAAT AGCAGCGCTA AAGAAACCCT GGAGCCATTA 
ATTCAGGCTG CTCAGTTACT GCAGGTGAAA AAGAAAACAG ATGAAGATGC AGAAGCCATC 
TGCTCCATGT GCAATGCTCT GACTACTGCC CAGATTGTCA AGGTGTTGAA CCTGTACACT 
CCAGTAAATG AGTTTGAAGA GCGGGTGTTG GTATCATTTA TACGGAATAT ACAGATGCGT 
TTAAGGGATA GGAAGGACTC CCCTCAGCTG CTAATGGATG CTAAGCATAT CTTCCCTGTC 
ACCTTCCCAT TCAACCCTTC GTCGCTTGCC CTAGAAACAA TTCAGATTCC TGGCAGTCTT 
GGCCTTGCCT TCCTCACACG TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5a ( XM_004912697.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912754.1 Xenopus tropicalis myosin VA (myo5a), transcript variant X4, mRNA.

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CloneID	OXa07366
Clone ID Related Accession (Same CDS sequence)	XM_004912697.3
Accession Version	XM_004912697.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912697.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTTATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC AGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGGA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACG GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAGA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ACACAATGAC CGATTCCATG GTCCTCTCTG AGGATGTACA GAAGATGAAG 
GATAAAAGGG AAATTGCTCA GGCCTACACT GGCATGAAGG AGACAAATAG GGTTTTGGAA 
TCCCAGCTGC AATCACAGAA AAAGGCCTTT GAGAATGAAG TGGAAGCTCT CAGAGGTGAG 
ATCCAGGCTC TAAAAGAGGA GAACAACCGG CAGCAGCAAC TTTTAGCCCA GAACCTCCAG 
TTGCCACCTG AAGCACGAAT CGAAGCCAGC TTGCAGCATG AAATCACCAG GCTAACCAAC 
GAGAACTTGG ATCTGATGGA GCAACTTGAA AAGCAAGATA AGACTGTCCG CAAACTGAAA 
AAGCAGTTGA AAGTCTTTGC CAAGAAAATT GGAGAGCTGG AAGTTGGTCA AATGGAAAAT 
GTTTCTCCTG GTCAGATAGT GGATGAACCC ATTCGTCCTG TTAATATTCC GCGCAAAGAG 
AAGGACTTCC AAGGAATGCT GGAATATAAG AAAGAGGATG AACTAAAGCT TGTCAAAAAC 
CTCATTTTAG AACTGAAGCC TCGTGGTGTC GCGGTCAACC TCATTCCAGG ACTGCCAGCA 
TATATCTTGT TCATGTGTTT GCGTCATGCT GATTACCTCA ATGATGATCA GAAAGTTCGC 
TCCTTGCTTA CATCAACTAT TAATGGTATC AAGAAAATCT TAAAGAAAAG AGGAGATGAT 
TTTGAAACGG TGTCCTTCTG GCTGTCCAAT TCGTGTCGGT TCCTCCATTG CTTGAAGCAG 
TACAGTGGAG AAGAGGGATT TATGAAGCAC AACACCACAC GGCAGAATGA GCACTGTCTG 
ACCAACTTTG ACCTTGCGGA ATACAGACAA GTCTTGAGTG ATCTGGCTAT TCAGATTTAC 
CAGCAGTTGG TCAGAGTGTT GGAAAATATC TTGCAGCCAA TGATTGTTTC AGGAATGCTG 
GAGCACGAGA CTATTCAGGG TGTCTCAGGG GTCAAGCCTA CCGGTCTTAG GAAGAGAACT 
TCCAGCATAG CAGATGAAGG GACGTACACA TTAGACTCCA TTGTGAGGCA GCTTAATACC 
TTCCATTCCA TAATGTGTCA GCATGGGATG GATCCTGAAC TCATCAAGCA GGTGGTCAAG 
CAAATGTTCT ACATCATCGG TGCTGTCACA CTCAACAACT TGCTCTTGCG AAAGGACATG 
TGCTCTTGGA GCAAAGGCAT GCAGATAAGG TACAACGTGA GCCAGTTGGA AGAATGGCTG 
CGTGACAAGA ACCTGATGAA TAGCAGCGCT AAAGAAACCC TGGAGCCATT AATTCAGGCT 
GCTCAGTTAC TGCAGGTGAA AAAGAAAACA GATGAAGATG CAGAAGCCAT CTGCTCCATG 
TGCAATGCTC TGACTACTGC CCAGATTGTC AAGGTGTTGA ACCTGTACAC TCCAGTAAAT 
GAGTTTGAAG AGCGGGTGTT GGTATCATTT ATACGGAATA TACAGATGCG TTTAAGGGAT 
AGGAAGGACT CCCCTCAGCT GCTAATGGAT GCTAAGCATA TCTTCCCTGT CACCTTCCCA 
TTCAACCCTT CGTCGCTTGC CCTAGAAACA ATTCAGATTC CTGGCAGTCT TGGCCTTGCC 
TTCCTCACAC GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912754.1
CDS	969..6446
Translation	MAASELYTKHARVWIPDPEEVWKSAEILKDYKPGDTVLRLRLEEGTDLEYRLDAKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGSDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASETNVEERVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRILGAHMRTYLLEKSRVVFQAEEERNYHIFYQLCASASLPEFKMLRLGTANDFHYTKQGGSPVIEGVDDQREMKNTRQACTLLGIGDSYQMGIFRILAAILHLGNVEFKSRDSDSCLVPPKHAPLNIFCDLMGVEYEEMSHWLCHRKLVTAAETYIKPISRLQATNARDALAKHIYAFLFNWIVCHVNKALHSSTKQNSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGILDLLDEECKMPKGSDSTWAQKLYNTHLKKSALFEKPRLSNVAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKASKFTLLTELFQEEEQILSPTSSAPPSGRTLLSRTGLRSLKPKPEQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKYPFTFDSKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRMACIRIQKTIRGWLLRKKYLRMRKAAITIQRYVRGYQARCYAQFLRRTRAAIIIQKFQRMYVVRQKYRHMQSITLALQSYMRGYAARKRFQGMLRAHKATIIQKHVRGWLARVTYKRNLSAIVYLQCCYRRMMAKRELKKLKIEARSVEHYKKLNNGMENKIMQLQRKVDEQNKDNKSLLERLTHLEVTYNTEKDKLRSDVDRLRHFEEEAKNAANRMVSLQDELARLRKELQQTQTEKNKIEERAEKYQTETEKLVAELREQNALLKAEKEELNLLIQEQAKKMTEDMEKKIIEETKQLELELNDERLRYQNLLNEYSRLEERYDDLKDEINTMSPPKPGHKRTDSTHSSNESEYTFSSEITESEDYPYRNEEPADRKTPLDMSLFLKLQKRVKELEQEKQVMQDDLDKKEEQIIRAKIMEEAKTPTRGTELEYESLKRQELESENKKLKNELNELRRAITEKASPDATGPGGTVYKVLLEQMTSVSEELEVRKEEVLILRSQLVSQMEAIPHKDTMTDSMVLSEDVQKMKDKREIAQAYTGMKETNRVLESQLQSQKKAFENEVEALRGEIQALKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENVSPGQIVDEPIRPVNIPRKEKDFQGMLEYKKEDELKLVKNLILELKPRGVAVNLIPGLPAYILFMCLRHADYLNDDQKVRSLLTSTINGIKKILKKRGDDFETVSFWLSNSCRFLHCLKQYSGEEGFMKHNTTRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIVRQLNTFHSIMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSSAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRNIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPGSLGLAFLTRV

Target ORF information:

RefSeq Version	XM_004912697.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VA (myo5a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912697.3

ORF Insert Sequence:

ATGGCAGCAT CCGAGCTCTA TACAAAGCAT GCAAGGGTTT GGATCCCTGA CCCGGAGGAA 
GTATGGAAAT CTGCAGAGAT CCTCAAGGAC TATAAGCCTG GGGACACTGT TCTTCGGTTA 
CGTTTGGAGG AAGGCACGGA TTTGGAATAC CGTCTCGATG CCAAGACAAA GGAGCTCCCC 
CCTCTGAGAA ACCCAGACAT CCTGGTGGGA GAGAATGACC TCACTGCCCT GAGTTATCTC 
CATGAGCCAG CTGTACTTCA CAATTTGAAA GTGCGATTTA TAGACTCCAA ACTTATTTAT 
ACCTACTGCG GCATTGTTCT TGTAGCTATA AACCCTTATG AGCAGTTACC CATCTATGGC 
TCAGACATCA TTAATGCATA CAGTGGGCAG AATATGGGGG ACATGGATCC CCATATTTTT 
GCGGTGGCGG AAGAAGCATA TAAACAAATG GCCAGGGATG AGCGCAATCA GTCGATTATT 
GTAAGTGGAG AATCTGGAGC AGGGAAAACG GTCTCTGCCA AGTATGCCAT GAGATACTTT 
GCAACAGTCA GTGGTTCTGC CAGTGAAACA AATGTTGAAG AAAGAGTGTT GGCCTCCAAT 
CCCATCATGG AGTCTATAGG TAATGCAAAA ACCACAAGGA ATGATAACAG CAGTCGCTTT 
GGCAAATACA TTGAAATTGG CTTTGATAAA CGCTACAGGA TCCTAGGTGC CCACATGAGA 
ACCTATTTGC TGGAAAAATC TAGAGTGGTG TTTCAGGCCG AAGAAGAGAG GAATTACCAC 
ATTTTCTATC AGCTCTGCGC ATCTGCTTCA CTGCCTGAGT TCAAGATGCT TCGACTGGGC 
ACTGCCAATG ATTTCCATTA CACAAAGCAA GGAGGCAGTC CAGTGATTGA AGGAGTTGAT 
GACCAGAGGG AAATGAAAAA CACAAGGCAA GCTTGCACCT TACTAGGAAT TGGAGACTCG 
TATCAGATGG GAATATTCAG AATTTTAGCT GCTATACTTC ACCTGGGTAA TGTGGAATTT 
AAGTCTCGGG ATTCAGATAG CTGTTTAGTC CCACCAAAAC ACGCACCCCT TAATATATTT 
TGTGACCTCA TGGGTGTGGA GTACGAAGAG ATGTCTCATT GGCTGTGTCA CAGGAAGCTT 
GTTACTGCTG CTGAGACCTA CATCAAGCCC ATTTCCAGGC TACAGGCCAC CAATGCCAGA 
GATGCCCTGG CTAAACATAT ATATGCTTTC TTGTTTAACT GGATTGTGTG TCATGTCAAC 
AAAGCTCTTC ATTCCTCAAC AAAGCAGAAT TCATTTATTG GAGTCCTTGA TATTTATGGG 
TTTGAAACAT TCGAAATCAA CAGCTTTGAA CAATTTTGTA TCAACTACGC TAACGAAAAG 
CTCCAGCAGC AGTTCAATTT GCATGTTTTT AAGTTGGAGC AAGAAGAATA TATGAAGGAG 
CAAATTCCAT GGACATTGAT TGATTTTTAT GATAACCAAC CTTGTATCAA TCTTATTGAA 
GCAAAAATGG GTATTCTGGA TCTGCTTGAT GAAGAATGCA AGATGCCCAA AGGGTCAGAC 
AGCACCTGGG CCCAGAAGTT ATACAATACT CATCTCAAAA AATCTGCATT ATTTGAAAAG 
CCGCGTCTGT CTAATGTGGC TTTTATTATC AAACACTTTG CAGACAAGGT AGAGTACCAG 
TGTGAAGGTT TTCTGGAAAA GAACAAAGAT ACAGTGTTTG AAGAACAAAT TAAAGTGCTG 
AAAGCAAGTA AGTTTACACT GCTAACAGAA CTGTTCCAAG AAGAGGAGCA GATCTTGAGC 
CCTACATCTT CTGCACCACC CTCAGGCCGT ACTCTGCTAT CCCGCACTGG TCTGCGTTCC 
CTCAAGCCCA AGCCTGAACA GACAAGCAAG GAGCATAAAA AAACTGTAGG ACATCAGTTC 
AGAAATTCCC TTCACCTTCT GATGGAGACT CTCAATGCCA CAACTCCACA TTATGTCCGT 
TGCATTAAGC CAAATGACTT CAAGTACCCG TTCACATTTG ACTCTAAACG AGCAGTGCAG 
CAGCTTAGGG CTTGTGGCGT CCTGGAAACC ATCCGAATCA GTGCTGCTGG ATTTCCTTCT 
AGGTGGACAT ATCAGGAATT TTTTAGCAGA TATCGTGTTT TAATGAAGCA AAAAGATGTC 
CTCAGTGATA GGAAGCAAAC CTGCAAAAAT GTTCTGGAGA AGCTTATTTT GGATAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAGATTTTT TTCCGTGCTG GCCAAGTGGC CTATCTGGAG 
AAGATCCGAG CAGACAAGCT GAGAATGGCC TGTATCAGGA TCCAGAAGAC AATCCGAGGA 
TGGCTTTTGA GGAAGAAATA CTTGCGCATG AGGAAAGCAG CCATAACAAT CCAGCGCTAC 
GTCAGAGGAT ACCAGGCACG ATGTTATGCA CAGTTTCTGC GTAGAACACG GGCCGCGATT 
ATTATCCAGA AATTCCAAAG GATGTATGTC GTTCGGCAGA AGTACCGTCA TATGCAGTCC 
ATTACCCTTG CTCTCCAGTC GTATATGCGT GGTTATGCAG CCAGGAAGAG ATTTCAGGGG 
ATGCTTAGAG CACACAAAGC TACCATCATC CAGAAGCATG TGCGCGGCTG GTTAGCCCGT 
GTCACTTATA AGAGAAATTT GAGTGCTATT GTGTACCTGC AGTGTTGTTA CCGCAGGATG 
ATGGCCAAAA GGGAGCTGAA GAAGTTAAAG ATAGAGGCAC GCTCAGTGGA ACACTACAAG 
AAACTCAACA ATGGGATGGA AAACAAGATC ATGCAGTTGC AGCGTAAAGT TGATGAGCAG 
AATAAAGACA ACAAATCCTT GCTGGAGAGG CTAACACACC TGGAGGTCAC GTACAATACT 
GAAAAAGATA AGCTGAGAAG CGACGTCGAC AGACTGCGCC ATTTTGAAGA GGAGGCCAAA 
AATGCAGCAA ACAGGATGGT CAGCTTGCAG GACGAGCTTG CCAGGCTTAG GAAAGAGCTG 
CAGCAAACAC AGACGGAAAA GAACAAGATT GAGGAACGGG CAGAGAAGTA CCAGACCGAG 
ACTGAAAAGC TGGTTGCTGA GCTGAGAGAA CAGAATGCTT TGCTGAAAGC TGAGAAAGAG 
GAACTGAATC TTTTAATCCA GGAGCAGGCA AAAAAAATGA CAGAGGATAT GGAAAAGAAA 
ATTATAGAAG AAACAAAGCA GTTGGAGCTT GAGCTGAATG ATGAACGACT GAGGTACCAG 
AATCTCCTAA ACGAGTACAG CCGCCTGGAG GAGCGATATG ATGACCTTAA AGATGAAATT 
AATACCATGA GTCCTCCAAA GCCAGGACAC AAGCGCACAG ACTCTACTCA TAGCAGCAAT 
GAATCAGAAT ACACTTTTAG TTCAGAGATC ACAGAGTCAG AAGATTACCC ATATCGAAAT 
GAGGAGCCTG CTGACAGAAA AACTCCGCTG GACATGTCCC TGTTCCTTAA GCTTCAGAAA 
CGGGTAAAGG AACTTGAGCA GGAAAAGCAA GTAATGCAAG ATGATTTGGA TAAGAAGGAG 
GAGCAGATAA TCCGAGCCAA AATAATGGAG GAGGCAAAGA CTCCAACTAG AGGTACAGAA 
CTGGAATATG AATCACTCAA GCGTCAAGAA CTGGAATCTG AAAATAAAAA ACTTAAGAAT 
GAGTTAAATG AGCTGCGAAG GGCAATCACT GAGAAAGCCT CCCCAGATGC TACAGGCCCT 
GGTGGAACTG TCTATAAAGT TCTCTTGGAG CAGATGACGT CAGTAAGTGA GGAACTTGAA 
GTACGCAAGG AAGAAGTCCT TATACTGAGG TCCCAGTTGG TGAGCCAAAT GGAAGCCATT 
CCACACAAGG ACACAATGAC CGATTCCATG GTCCTCTCTG AGGATGTACA GAAGATGAAG 
GATAAAAGGG AAATTGCTCA GGCCTACACT GGCATGAAGG AGACAAATAG GGTTTTGGAA 
TCCCAGCTGC AATCACAGAA AAAGGCCTTT GAGAATGAAG TGGAAGCTCT CAGAGGTGAG 
ATCCAGGCTC TAAAAGAGGA GAACAACCGG CAGCAGCAAC TTTTAGCCCA GAACCTCCAG 
TTGCCACCTG AAGCACGAAT CGAAGCCAGC TTGCAGCATG AAATCACCAG GCTAACCAAC 
GAGAACTTGG ATCTGATGGA GCAACTTGAA AAGCAAGATA AGACTGTCCG CAAACTGAAA 
AAGCAGTTGA AAGTCTTTGC CAAGAAAATT GGAGAGCTGG AAGTTGGTCA AATGGAAAAT 
GTTTCTCCTG GTCAGATAGT GGATGAACCC ATTCGTCCTG TTAATATTCC GCGCAAAGAG 
AAGGACTTCC AAGGAATGCT GGAATATAAG AAAGAGGATG AACTAAAGCT TGTCAAAAAC 
CTCATTTTAG AACTGAAGCC TCGTGGTGTC GCGGTCAACC TCATTCCAGG ACTGCCAGCA 
TATATCTTGT TCATGTGTTT GCGTCATGCT GATTACCTCA ATGATGATCA GAAAGTTCGC 
TCCTTGCTTA CATCAACTAT TAATGGTATC AAGAAAATCT TAAAGAAAAG AGGAGATGAT 
TTTGAAACGG TGTCCTTCTG GCTGTCCAAT TCGTGTCGGT TCCTCCATTG CTTGAAGCAG 
TACAGTGGAG AAGAGGGATT TATGAAGCAC AACACCACAC GGCAGAATGA GCACTGTCTG 
ACCAACTTTG ACCTTGCGGA ATACAGACAA GTCTTGAGTG ATCTGGCTAT TCAGATTTAC 
CAGCAGTTGG TCAGAGTGTT GGAAAATATC TTGCAGCCAA TGATTGTTTC AGGAATGCTG 
GAGCACGAGA CTATTCAGGG TGTCTCAGGG GTCAAGCCTA CCGGTCTTAG GAAGAGAACT 
TCCAGCATAG CAGATGAAGG GACGTACACA TTAGACTCCA TTGTGAGGCA GCTTAATACC 
TTCCATTCCA TAATGTGTCA GCATGGGATG GATCCTGAAC TCATCAAGCA GGTGGTCAAG 
CAAATGTTCT ACATCATCGG TGCTGTCACA CTCAACAACT TGCTCTTGCG AAAGGACATG 
TGCTCTTGGA GCAAAGGCAT GCAGATAAGG TACAACGTGA GCCAGTTGGA AGAATGGCTG 
CGTGACAAGA ACCTGATGAA TAGCAGCGCT AAAGAAACCC TGGAGCCATT AATTCAGGCT 
GCTCAGTTAC TGCAGGTGAA AAAGAAAACA GATGAAGATG CAGAAGCCAT CTGCTCCATG 
TGCAATGCTC TGACTACTGC CCAGATTGTC AAGGTGTTGA ACCTGTACAC TCCAGTAAAT 
GAGTTTGAAG AGCGGGTGTT GGTATCATTT ATACGGAATA TACAGATGCG TTTAAGGGAT 
AGGAAGGACT CCCCTCAGCT GCTAATGGAT GCTAAGCATA TCTTCCCTGT CACCTTCCCA 
TTCAACCCTT CGTCGCTTGC CCTAGAAACA ATTCAGATTC CTGGCAGTCT TGGCCTTGCC 
TTCCTCACAC GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	myo5a
Entrez Gene ID	100495365
Full Name	myosin VA
General protein information	Preferred Names myosin VA Names unconventional myosin-Va myosin VA (heavy chain 12, myoxin)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	MYO5A	XP_001084476.1
Canis lupus familiaris (dog)	MYO5A	XP_005638745.1
Mus musculus (house mouse)	Myo5a	NP_034994.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006479	XP_316519.4
Kluyveromyces lactis	KLLA0A03905g	XP_451171.1
Magnaporthe oryzae (rice blast fungus)	MGG_16213	XP_003709659.1
Arabidopsis thaliana (thale cress)	XIC	NP_172349.2
Oryza sativa (rice)	Os06g0488200	NP_001057670.1
Arabidopsis thaliana (thale cress)	XIE	NP_175858.1
Bos taurus (cattle)	MYO5A	XP_005211933.1
Rattus norvegicus (Norway rat)	Myo5a	NP_071514.1
Gallus gallus (chicken)	MYO5A	NP_990631.1
Neurospora crassa	NCU11354	XP_001728528.1
Xenopus tropicalis (tropical clawed frog)	myo5a	XP_004912752.1
Pan troglodytes (chimpanzee)	MYO5A	XP_510412.2
Danio rerio (zebrafish)	myo5aa	XP_005173731.1
Drosophila melanogaster (fruit fly)	didum	NP_724569.1
Caenorhabditis elegans (roundworm)	hum-2	NP_001023886.1
Saccharomyces cerevisiae (baker's yeast)	MYO2	NP_014971.1
Homo sapiens (human)	MYO5A	NP_000250.3
Eremothecium gossypii	AGOS_ADR354W	NP_984450.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

myo5a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

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