Myo5a gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006510831.3	XP_006510894.1	unconventional myosin-Va isoform X5
XM_006510827.3	XP_006510890.1	unconventional myosin-Va isoform X1
XM_006510827.4	XP_006510890.1	unconventional myosin-Va isoform X1
XM_006510832.4	XP_006510895.1	unconventional myosin-Va isoform X6
XM_006510829.3	XP_006510892.1	unconventional myosin-Va isoform X3
XM_006510828.4	XP_006510891.1	unconventional myosin-Va isoform X2
XM_006510830.4	XP_006510893.1	unconventional myosin-Va isoform X4
NM_010864.2	NP_034994.2	unconventional myosin-Va
XM_006510828.3	XP_006510891.1	unconventional myosin-Va isoform X2
XM_006510829.4	XP_006510892.1	unconventional myosin-Va isoform X3
XM_006510830.3	XP_006510893.1	unconventional myosin-Va isoform X4
XM_006510832.3	XP_006510895.1	unconventional myosin-Va isoform X6
XM_006510834.3	XP_006510897.1	unconventional myosin-Va isoform X7
XM_006510831.4	XP_006510894.1	unconventional myosin-Va isoform X5
XM_030244103.1	XP_030099963.1	unconventional myosin-Va isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu42561	XM_006510834.3 Latest version!	Mus musculus myosin VA (Myo5a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu42554	XM_006510827.4 Latest version!	Mus musculus myosin VA (Myo5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu42555	XM_006510828.4 Latest version!	Mus musculus myosin VA (Myo5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu42556	XM_006510829.4 Latest version!	Mus musculus myosin VA (Myo5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu42557	XM_006510830.4 Latest version!	Mus musculus myosin VA (Myo5a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu42558	XM_006510831.4 Latest version!	Mus musculus myosin VA (Myo5a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu42559	XM_006510832.4 Latest version!	Mus musculus myosin VA (Myo5a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu90090	XM_030244103.1 Latest version!	Mus musculus myosin VA (Myo5a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu22050	NM_010864.2 Latest version!	Mus musculus myosin VA (Myo5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1364.30 $1949.00
View Old Accession Versions >>
OMu42554	XM_006510827.3	Mus musculus myosin VA (Myo5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu42555	XM_006510828.3	Mus musculus myosin VA (Myo5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu42556	XM_006510829.3	Mus musculus myosin VA (Myo5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu42557	XM_006510830.3	Mus musculus myosin VA (Myo5a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu42559	XM_006510832.3	Mus musculus myosin VA (Myo5a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu42558	XM_006510831.3	Mus musculus myosin VA (Myo5a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

Myo5a ( XM_006510834.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510897.1 Mus musculus myosin VA (Myo5a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu42561
Clone ID Related Accession (Same CDS sequence)	XM_006510834.3
Accession Version	XM_006510834.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510834.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGGG CAGCCATCAC AGTGCAGCGA TACGTGCGGG GCTATCAGGC TCGATGCTAT 
GCTAAGTTTC TGCGCAGAAC CAAGGCAGCA ACCACCATTC AAAAGTACTG GCGCATGTAT 
GTGGTCCGCA GGAGGTACAA GATTAGACGA GCTGCCACGA TTGTTATTCA GTCTTACTTG 
AGAGGCTACT TGACAAGAAA TAGGTATCGC AAGATACTCC GTGAATACAA AGCAGTCATC 
ATTCAGAAAC GTGTCCGTGG CTGGCTGGCC CGTACACATT ATAAGAGGAC CATGAAAGCC 
ATCGTCTACC TTCAGTGCTG CTTCCGGCGG ATGATGGCCA AGCGTGAGCT GAAGAAACTC 
AAAATTGAGG CTCGCTCTGT GGAACGCTAC AAGAAGCTCC ATATTGGCAT GGAGAACAAG 
ATTATGCAGC TGCAGCGCAA AGTGGATGAG CAGAATAAAG ACTACAAATG CCTCATGGAG 
AAACTGACCA ATCTGGAAGG AGTATACAAC TCTGAGACTG AAAAACTACG AAATGATGTA 
GAACGTCTTC AGCTAAGTGA AGAGGAAGCT AAGGTTGCCA CTGGGCGGGT CCTTAGTCTG 
CAGGAAGAAA TTGCAAAACT CCGAAAAGAC CTGGAACAAA CTCGATCAGA GAAAAAGTCT 
ATTGAAGAAA GAGCAGATAA ATACAAACAA GAAACTGACC AGCTGGTGTC AAACTTGAAG 
GAAGAAAATA CTTTGCTGAA GCAGGAAAAG GAGACCCTCA ACCACCGCAT TGTGGAGCAG 
GCGAAGGAGA TGACAGAAAC TATGGAGAGG AAGTTAGTAG AAGAGACAAA ACAACTGGAG 
CTTGACCTGA ATGATGAGAG GCTGAGGTAT CAGAACCTTC TGAATGAGTT CAGTCGCCTG 
GAGGAGCGCT ATGATGACCT CAAGGAAGAG ATGACCCTGA TGCTGAATGT GCCTAAGCCA 
GGACACAAGA GAACAGACTC TACCCACAGC AGTAATGAGT CTGAATACAC CTTCAGCTCT 
GAGTTTGCAG AAACTGAAGA CATTGCACCA AGAACAGAGG AGCCAATTGA GAAGAAGGTG 
CCTCTGGATA TGTCACTATT CCTTAAGCTC CAGAAGCGTG TCACAGAGCT GGAACAGGAG 
AAGCAGCTGA TGCAGGATGA GCTGGACCGC AAGGAGGAGC AGGTGTTCCG CAGCAAGGCA 
AAGGAAGAAG AAAGGCCACA GATAAGAGGA GCTGAACTAG AGTATGAGTC TCTCAAGCGT 
CAAGAACTGG AGTCAGAAAA CAAAAAACTG AAAAACGAGC TGAATGAGTT GCGCAAAGCC 
CTCAGTGAGA AAAGTGCCCC AGAAGTGACT GCGCCAGGTG CGCCTGCTTA CCGAGTCCTC 
ATGGAACAGC TGACCTCCGT GAGCGAGGAG CTCGACGTGC GCAAGGAGGA AGTCCTCATC 
TTGAGGTCGC AGCTGGTGAG CCAAAAAGAA GCCATCCAAC CCAAGGATGA CAAGAATACA 
ATGACAGATT CCACAATTCT TTTAGAAGAT GTACAGAAAA TGAAAGACAA AGGTGAAATA 
GCACAAGCAT ATATTGGTTT GAAAGAAACA AACAGATCAT CTACTATGGA TTACCAGGAG 
TTGAATGAGG ATGGAGAGCT CTGGATGGTT TATGAAGGGT TAAAACAAGC CAACAGGCTC 
TTAGAATCCC AGCTACAGTC ACAGAAAAGA AGCCATGAGA ATGAGGCTGA GGCCCTCCGT 
GGGGAGATCC AGAGCCTAAA GGAAGAAAAC AACCGGCAAC AGCAGCTGCT GGCCCAGAAC 
CTGCAGCTGC CCCCTGAGGC CCGCATTGAG GCCAGCCTGC AGCATGAGAT CACCCGGCTG 
ACCAATGAAA ACCTGTATTT TGAGGAATTA TATGCAGATG ACCCTAAGAA GTATCAATCC 
TATCGGATTT CACTTTACAA AAGGATGATT GATCTGATGG AACAACTTGA AAAGCAGGAT 
AAAACTGTCC GGAAACTGAA GAAACAACTG AAAGTCTTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGGC AGATGGAGAA CATATCTCCA GGACAGATCA TCGATGAGCC TATCCGGCCA 
GTCAACATTC CCCGGAAAGA AAAGGATTTC CAAGGAATGC TGGAGTACAA AAGGGAGGAT 
GAGCAGAAGC TAGTGAAGAA CCTGATTCTA GAACTAAAGC CACGTGGTGT GGCTGTCAAT 
CTGATTCCAG GGTTACCAGC ATATATCTTG TTTATGTGTG TACGACATGC TGACTATCTG 
AACGATGATC AGAAAGTAAG ATCATTGCTG ACATCAACAA TTAACAGCAT CAAAAAAGTC 
TTGAAGAAAA GAGGTGACGA TTTTGAAACT GTCTCCTTCT GGCTCTCTAA CACATGTCGA 
TTTTTGCACT GTTTGAAGCA ATATAGTGGA GAGGAGGGCT TTATGAAGCA CAACACGTCT 
CGCCAGAATG AGCACTGCCT CACCAATTTT GACCTTGCTG AATATCGGCA AGTACTGAGT 
GACTTGGCCA TTCAGATCTA TCAGCAGCTT GTGAGGGTGT TAGAGAACAT TCTTCAGCCA 
ATGATAGTCT CAGGTATGCT AGAGCATGAA ACAATTCAGG GCGTATCTGG GGTGAAGCCC 
ACAGGGCTCA GAAAACGAAC CTCCAGTATC GCAGATGAGG GCACCTACAC ACTGGACTCC 
ATCCTTCGGC AGCTCAACTC CTTCCACTCA GTCATGTGTC AGCATGGCAT GGACCCGGAG 
CTAATCAAGC AGGTAGTCAA ACAGATGTTC TACATTGTGG GCGCCATCAC CCTAAACAAC 
CTCCTCCTGC GCAAGGACAT GTGCTCCTGG AGCAAGGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTGG AAGAATGGCT ACGTGACAAG AATCTAATGA ACAGTGGGGC CAAGGAGACT 
CTGGAACCTC TTATCCAGGC CGCTCAGCTT TTGCAAGTGA AAAAGAAAAC TGACGATGAC 
GCAGAAGCCA TCTGTTCCAT GTGCAACGCC CTGACCACGG CCCAGATTGT CAAAGTGTTG 
AATCTGTACA CACCGGTTAA TGAGTTTGAA GAAAGGGTCT CAGTTTCATT TATCCGCACT 
ATACAGATGC GGTTACGAGA CAGGAAAGAC TCTCCACAGC TGCTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACTTTTCC CTTTAACCCA TCCTCCCTAG CCCTAGAAAC CATCCAGATC 
CCAGCCAGCC TGGGCCTGGG CTTCATCGCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510897.1
CDS	254..3532
Translation	MQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSTMDYQELNEDGELWMVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510834.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510834.3

ORF Insert Sequence:

ATGCAGAGGG CAGCCATCAC AGTGCAGCGA TACGTGCGGG GCTATCAGGC TCGATGCTAT 
GCTAAGTTTC TGCGCAGAAC CAAGGCAGCA ACCACCATTC AAAAGTACTG GCGCATGTAT 
GTGGTCCGCA GGAGGTACAA GATTAGACGA GCTGCCACGA TTGTTATTCA GTCTTACTTG 
AGAGGCTACT TGACAAGAAA TAGGTATCGC AAGATACTCC GTGAATACAA AGCAGTCATC 
ATTCAGAAAC GTGTCCGTGG CTGGCTGGCC CGTACACATT ATAAGAGGAC CATGAAAGCC 
ATCGTCTACC TTCAGTGCTG CTTCCGGCGG ATGATGGCCA AGCGTGAGCT GAAGAAACTC 
AAAATTGAGG CTCGCTCTGT GGAACGCTAC AAGAAGCTCC ATATTGGCAT GGAGAACAAG 
ATTATGCAGC TGCAGCGCAA AGTGGATGAG CAGAATAAAG ACTACAAATG CCTCATGGAG 
AAACTGACCA ATCTGGAAGG AGTATACAAC TCTGAGACTG AAAAACTACG AAATGATGTA 
GAACGTCTTC AGCTAAGTGA AGAGGAAGCT AAGGTTGCCA CTGGGCGGGT CCTTAGTCTG 
CAGGAAGAAA TTGCAAAACT CCGAAAAGAC CTGGAACAAA CTCGATCAGA GAAAAAGTCT 
ATTGAAGAAA GAGCAGATAA ATACAAACAA GAAACTGACC AGCTGGTGTC AAACTTGAAG 
GAAGAAAATA CTTTGCTGAA GCAGGAAAAG GAGACCCTCA ACCACCGCAT TGTGGAGCAG 
GCGAAGGAGA TGACAGAAAC TATGGAGAGG AAGTTAGTAG AAGAGACAAA ACAACTGGAG 
CTTGACCTGA ATGATGAGAG GCTGAGGTAT CAGAACCTTC TGAATGAGTT CAGTCGCCTG 
GAGGAGCGCT ATGATGACCT CAAGGAAGAG ATGACCCTGA TGCTGAATGT GCCTAAGCCA 
GGACACAAGA GAACAGACTC TACCCACAGC AGTAATGAGT CTGAATACAC CTTCAGCTCT 
GAGTTTGCAG AAACTGAAGA CATTGCACCA AGAACAGAGG AGCCAATTGA GAAGAAGGTG 
CCTCTGGATA TGTCACTATT CCTTAAGCTC CAGAAGCGTG TCACAGAGCT GGAACAGGAG 
AAGCAGCTGA TGCAGGATGA GCTGGACCGC AAGGAGGAGC AGGTGTTCCG CAGCAAGGCA 
AAGGAAGAAG AAAGGCCACA GATAAGAGGA GCTGAACTAG AGTATGAGTC TCTCAAGCGT 
CAAGAACTGG AGTCAGAAAA CAAAAAACTG AAAAACGAGC TGAATGAGTT GCGCAAAGCC 
CTCAGTGAGA AAAGTGCCCC AGAAGTGACT GCGCCAGGTG CGCCTGCTTA CCGAGTCCTC 
ATGGAACAGC TGACCTCCGT GAGCGAGGAG CTCGACGTGC GCAAGGAGGA AGTCCTCATC 
TTGAGGTCGC AGCTGGTGAG CCAAAAAGAA GCCATCCAAC CCAAGGATGA CAAGAATACA 
ATGACAGATT CCACAATTCT TTTAGAAGAT GTACAGAAAA TGAAAGACAA AGGTGAAATA 
GCACAAGCAT ATATTGGTTT GAAAGAAACA AACAGATCAT CTACTATGGA TTACCAGGAG 
TTGAATGAGG ATGGAGAGCT CTGGATGGTT TATGAAGGGT TAAAACAAGC CAACAGGCTC 
TTAGAATCCC AGCTACAGTC ACAGAAAAGA AGCCATGAGA ATGAGGCTGA GGCCCTCCGT 
GGGGAGATCC AGAGCCTAAA GGAAGAAAAC AACCGGCAAC AGCAGCTGCT GGCCCAGAAC 
CTGCAGCTGC CCCCTGAGGC CCGCATTGAG GCCAGCCTGC AGCATGAGAT CACCCGGCTG 
ACCAATGAAA ACCTGTATTT TGAGGAATTA TATGCAGATG ACCCTAAGAA GTATCAATCC 
TATCGGATTT CACTTTACAA AAGGATGATT GATCTGATGG AACAACTTGA AAAGCAGGAT 
AAAACTGTCC GGAAACTGAA GAAACAACTG AAAGTCTTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGGC AGATGGAGAA CATATCTCCA GGACAGATCA TCGATGAGCC TATCCGGCCA 
GTCAACATTC CCCGGAAAGA AAAGGATTTC CAAGGAATGC TGGAGTACAA AAGGGAGGAT 
GAGCAGAAGC TAGTGAAGAA CCTGATTCTA GAACTAAAGC CACGTGGTGT GGCTGTCAAT 
CTGATTCCAG GGTTACCAGC ATATATCTTG TTTATGTGTG TACGACATGC TGACTATCTG 
AACGATGATC AGAAAGTAAG ATCATTGCTG ACATCAACAA TTAACAGCAT CAAAAAAGTC 
TTGAAGAAAA GAGGTGACGA TTTTGAAACT GTCTCCTTCT GGCTCTCTAA CACATGTCGA 
TTTTTGCACT GTTTGAAGCA ATATAGTGGA GAGGAGGGCT TTATGAAGCA CAACACGTCT 
CGCCAGAATG AGCACTGCCT CACCAATTTT GACCTTGCTG AATATCGGCA AGTACTGAGT 
GACTTGGCCA TTCAGATCTA TCAGCAGCTT GTGAGGGTGT TAGAGAACAT TCTTCAGCCA 
ATGATAGTCT CAGGTATGCT AGAGCATGAA ACAATTCAGG GCGTATCTGG GGTGAAGCCC 
ACAGGGCTCA GAAAACGAAC CTCCAGTATC GCAGATGAGG GCACCTACAC ACTGGACTCC 
ATCCTTCGGC AGCTCAACTC CTTCCACTCA GTCATGTGTC AGCATGGCAT GGACCCGGAG 
CTAATCAAGC AGGTAGTCAA ACAGATGTTC TACATTGTGG GCGCCATCAC CCTAAACAAC 
CTCCTCCTGC GCAAGGACAT GTGCTCCTGG AGCAAGGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTGG AAGAATGGCT ACGTGACAAG AATCTAATGA ACAGTGGGGC CAAGGAGACT 
CTGGAACCTC TTATCCAGGC CGCTCAGCTT TTGCAAGTGA AAAAGAAAAC TGACGATGAC 
GCAGAAGCCA TCTGTTCCAT GTGCAACGCC CTGACCACGG CCCAGATTGT CAAAGTGTTG 
AATCTGTACA CACCGGTTAA TGAGTTTGAA GAAAGGGTCT CAGTTTCATT TATCCGCACT 
ATACAGATGC GGTTACGAGA CAGGAAAGAC TCTCCACAGC TGCTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACTTTTCC CTTTAACCCA TCCTCCCTAG CCCTAGAAAC CATCCAGATC 
CCAGCCAGCC TGGGCCTGGG CTTCATCGCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_006510827.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510890.1 Mus musculus myosin VA (Myo5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu42554
Clone ID Related Accession (Same CDS sequence)	XM_006510827.3 , XM_006510827.4
Accession Version	XM_006510827.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510827.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGA 
TCATCTACTA TGGATTACCA GGAGTTGAAT GAGGATGGAG AGCTCTGGAT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCTTAGAA TCCCAGCTAC AGTCACAGAA AAGAAGCCAT 
GAGAATGAGG CTGAGGCCCT CCGTGGGGAG ATCCAGAGCC TAAAGGAAGA AAACAACCGG 
CAACAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCATG AGATCACCCG GCTGACCAAT GAAAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCCTATCGG ATTTCACTTT ACAAAAGGAT GATTGATCTG 
ATGGAACAAC TTGAAAAGCA GGATAAAACT GTCCGGAAAC TGAAGAAACA ACTGAAAGTC 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGGCAGATGG AGAACATATC TCCAGGACAG 
ATCATCGATG AGCCTATCCG GCCAGTCAAC ATTCCCCGGA AAGAAAAGGA TTTCCAAGGA 
ATGCTGGAGT ACAAAAGGGA GGATGAGCAG AAGCTAGTGA AGAACCTGAT TCTAGAACTA 
AAGCCACGTG GTGTGGCTGT CAATCTGATT CCAGGGTTAC CAGCATATAT CTTGTTTATG 
TGTGTACGAC ATGCTGACTA TCTGAACGAT GATCAGAAAG TAAGATCATT GCTGACATCA 
ACAATTAACA GCATCAAAAA AGTCTTGAAG AAAAGAGGTG ACGATTTTGA AACTGTCTCC 
TTCTGGCTCT CTAACACATG TCGATTTTTG CACTGTTTGA AGCAATATAG TGGAGAGGAG 
GGCTTTATGA AGCACAACAC GTCTCGCCAG AATGAGCACT GCCTCACCAA TTTTGACCTT 
GCTGAATATC GGCAAGTACT GAGTGACTTG GCCATTCAGA TCTATCAGCA GCTTGTGAGG 
GTGTTAGAGA ACATTCTTCA GCCAATGATA GTCTCAGGTA TGCTAGAGCA TGAAACAATT 
CAGGGCGTAT CTGGGGTGAA GCCCACAGGG CTCAGAAAAC GAACCTCCAG TATCGCAGAT 
GAGGGCACCT ACACACTGGA CTCCATCCTT CGGCAGCTCA ACTCCTTCCA CTCAGTCATG 
TGTCAGCATG GCATGGACCC GGAGCTAATC AAGCAGGTAG TCAAACAGAT GTTCTACATT 
GTGGGCGCCA TCACCCTAAA CAACCTCCTC CTGCGCAAGG ACATGTGCTC CTGGAGCAAG 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTGGAAGAAT GGCTACGTGA CAAGAATCTA 
ATGAACAGTG GGGCCAAGGA GACTCTGGAA CCTCTTATCC AGGCCGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACTGACGA TGACGCAGAA GCCATCTGTT CCATGTGCAA CGCCCTGACC 
ACGGCCCAGA TTGTCAAAGT GTTGAATCTG TACACACCGG TTAATGAGTT TGAAGAAAGG 
GTCTCAGTTT CATTTATCCG CACTATACAG ATGCGGTTAC GAGACAGGAA AGACTCTCCA 
CAGCTGCTCA TGGATGCAAA ACACATCTTT CCTGTCACTT TTCCCTTTAA CCCATCCTCC 
CTAGCCCTAG AAACCATCCA GATCCCAGCC AGCCTGGGCC TGGGCTTCAT CGCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510890.1
CDS	1835..7477
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSTMDYQELNEDGELWMVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510827.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510827.4

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGA 
TCATCTACTA TGGATTACCA GGAGTTGAAT GAGGATGGAG AGCTCTGGAT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCTTAGAA TCCCAGCTAC AGTCACAGAA AAGAAGCCAT 
GAGAATGAGG CTGAGGCCCT CCGTGGGGAG ATCCAGAGCC TAAAGGAAGA AAACAACCGG 
CAACAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCATG AGATCACCCG GCTGACCAAT GAAAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCCTATCGG ATTTCACTTT ACAAAAGGAT GATTGATCTG 
ATGGAACAAC TTGAAAAGCA GGATAAAACT GTCCGGAAAC TGAAGAAACA ACTGAAAGTC 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGGCAGATGG AGAACATATC TCCAGGACAG 
ATCATCGATG AGCCTATCCG GCCAGTCAAC ATTCCCCGGA AAGAAAAGGA TTTCCAAGGA 
ATGCTGGAGT ACAAAAGGGA GGATGAGCAG AAGCTAGTGA AGAACCTGAT TCTAGAACTA 
AAGCCACGTG GTGTGGCTGT CAATCTGATT CCAGGGTTAC CAGCATATAT CTTGTTTATG 
TGTGTACGAC ATGCTGACTA TCTGAACGAT GATCAGAAAG TAAGATCATT GCTGACATCA 
ACAATTAACA GCATCAAAAA AGTCTTGAAG AAAAGAGGTG ACGATTTTGA AACTGTCTCC 
TTCTGGCTCT CTAACACATG TCGATTTTTG CACTGTTTGA AGCAATATAG TGGAGAGGAG 
GGCTTTATGA AGCACAACAC GTCTCGCCAG AATGAGCACT GCCTCACCAA TTTTGACCTT 
GCTGAATATC GGCAAGTACT GAGTGACTTG GCCATTCAGA TCTATCAGCA GCTTGTGAGG 
GTGTTAGAGA ACATTCTTCA GCCAATGATA GTCTCAGGTA TGCTAGAGCA TGAAACAATT 
CAGGGCGTAT CTGGGGTGAA GCCCACAGGG CTCAGAAAAC GAACCTCCAG TATCGCAGAT 
GAGGGCACCT ACACACTGGA CTCCATCCTT CGGCAGCTCA ACTCCTTCCA CTCAGTCATG 
TGTCAGCATG GCATGGACCC GGAGCTAATC AAGCAGGTAG TCAAACAGAT GTTCTACATT 
GTGGGCGCCA TCACCCTAAA CAACCTCCTC CTGCGCAAGG ACATGTGCTC CTGGAGCAAG 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTGGAAGAAT GGCTACGTGA CAAGAATCTA 
ATGAACAGTG GGGCCAAGGA GACTCTGGAA CCTCTTATCC AGGCCGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACTGACGA TGACGCAGAA GCCATCTGTT CCATGTGCAA CGCCCTGACC 
ACGGCCCAGA TTGTCAAAGT GTTGAATCTG TACACACCGG TTAATGAGTT TGAAGAAAGG 
GTCTCAGTTT CATTTATCCG CACTATACAG ATGCGGTTAC GAGACAGGAA AGACTCTCCA 
CAGCTGCTCA TGGATGCAAA ACACATCTTT CCTGTCACTT TTCCCTTTAA CCCATCCTCC 
CTAGCCCTAG AAACCATCCA GATCCCAGCC AGCCTGGGCC TGGGCTTCAT CGCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_006510828.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510891.1 Mus musculus myosin VA (Myo5a), transcript variant X2, mRNA.

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CloneID	OMu42555
Clone ID Related Accession (Same CDS sequence)	XM_006510828.4 , XM_006510828.3
Accession Version	XM_006510828.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510828.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG ATCATCTACT 
ATGGATTACC AGGAGTTGAA TGAGGATGGA GAGCTCTGGA TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCTTAGA ATCCCAGCTA CAGTCACAGA AAAGAAGCCA TGAGAATGAG 
GCTGAGGCCC TCCGTGGGGA GATCCAGAGC CTAAAGGAAG AAAACAACCG GCAACAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCT GAGGCCCGCA TTGAGGCCAG CCTGCAGCAT 
GAGATCACCC GGCTGACCAA TGAAAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCCTATCG GATTTCACTT TACAAAAGGA TGATTGATCT GATGGAACAA 
CTTGAAAAGC AGGATAAAAC TGTCCGGAAA CTGAAGAAAC AACTGAAAGT CTTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGGCAGATG GAGAACATAT CTCCAGGACA GATCATCGAT 
GAGCCTATCC GGCCAGTCAA CATTCCCCGG AAAGAAAAGG ATTTCCAAGG AATGCTGGAG 
TACAAAAGGG AGGATGAGCA GAAGCTAGTG AAGAACCTGA TTCTAGAACT AAAGCCACGT 
GGTGTGGCTG TCAATCTGAT TCCAGGGTTA CCAGCATATA TCTTGTTTAT GTGTGTACGA 
CATGCTGACT ATCTGAACGA TGATCAGAAA GTAAGATCAT TGCTGACATC AACAATTAAC 
AGCATCAAAA AAGTCTTGAA GAAAAGAGGT GACGATTTTG AAACTGTCTC CTTCTGGCTC 
TCTAACACAT GTCGATTTTT GCACTGTTTG AAGCAATATA GTGGAGAGGA GGGCTTTATG 
AAGCACAACA CGTCTCGCCA GAATGAGCAC TGCCTCACCA ATTTTGACCT TGCTGAATAT 
CGGCAAGTAC TGAGTGACTT GGCCATTCAG ATCTATCAGC AGCTTGTGAG GGTGTTAGAG 
AACATTCTTC AGCCAATGAT AGTCTCAGGT ATGCTAGAGC ATGAAACAAT TCAGGGCGTA 
TCTGGGGTGA AGCCCACAGG GCTCAGAAAA CGAACCTCCA GTATCGCAGA TGAGGGCACC 
TACACACTGG ACTCCATCCT TCGGCAGCTC AACTCCTTCC ACTCAGTCAT GTGTCAGCAT 
GGCATGGACC CGGAGCTAAT CAAGCAGGTA GTCAAACAGA TGTTCTACAT TGTGGGCGCC 
ATCACCCTAA ACAACCTCCT CCTGCGCAAG GACATGTGCT CCTGGAGCAA GGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTGGAAGAA TGGCTACGTG ACAAGAATCT AATGAACAGT 
GGGGCCAAGG AGACTCTGGA ACCTCTTATC CAGGCCGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACTGACG ATGACGCAGA AGCCATCTGT TCCATGTGCA ACGCCCTGAC CACGGCCCAG 
ATTGTCAAAG TGTTGAATCT GTACACACCG GTTAATGAGT TTGAAGAAAG GGTCTCAGTT 
TCATTTATCC GCACTATACA GATGCGGTTA CGAGACAGGA AAGACTCTCC ACAGCTGCTC 
ATGGATGCAA AACACATCTT TCCTGTCACT TTTCCCTTTA ACCCATCCTC CCTAGCCCTA 
GAAACCATCC AGATCCCAGC CAGCCTGGGC CTGGGCTTCA TCGCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510891.1
CDS	1835..7468
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSTMDYQELNEDGELWMVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510828.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510828.4

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG ATCATCTACT 
ATGGATTACC AGGAGTTGAA TGAGGATGGA GAGCTCTGGA TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCTTAGA ATCCCAGCTA CAGTCACAGA AAAGAAGCCA TGAGAATGAG 
GCTGAGGCCC TCCGTGGGGA GATCCAGAGC CTAAAGGAAG AAAACAACCG GCAACAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCT GAGGCCCGCA TTGAGGCCAG CCTGCAGCAT 
GAGATCACCC GGCTGACCAA TGAAAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCCTATCG GATTTCACTT TACAAAAGGA TGATTGATCT GATGGAACAA 
CTTGAAAAGC AGGATAAAAC TGTCCGGAAA CTGAAGAAAC AACTGAAAGT CTTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGGCAGATG GAGAACATAT CTCCAGGACA GATCATCGAT 
GAGCCTATCC GGCCAGTCAA CATTCCCCGG AAAGAAAAGG ATTTCCAAGG AATGCTGGAG 
TACAAAAGGG AGGATGAGCA GAAGCTAGTG AAGAACCTGA TTCTAGAACT AAAGCCACGT 
GGTGTGGCTG TCAATCTGAT TCCAGGGTTA CCAGCATATA TCTTGTTTAT GTGTGTACGA 
CATGCTGACT ATCTGAACGA TGATCAGAAA GTAAGATCAT TGCTGACATC AACAATTAAC 
AGCATCAAAA AAGTCTTGAA GAAAAGAGGT GACGATTTTG AAACTGTCTC CTTCTGGCTC 
TCTAACACAT GTCGATTTTT GCACTGTTTG AAGCAATATA GTGGAGAGGA GGGCTTTATG 
AAGCACAACA CGTCTCGCCA GAATGAGCAC TGCCTCACCA ATTTTGACCT TGCTGAATAT 
CGGCAAGTAC TGAGTGACTT GGCCATTCAG ATCTATCAGC AGCTTGTGAG GGTGTTAGAG 
AACATTCTTC AGCCAATGAT AGTCTCAGGT ATGCTAGAGC ATGAAACAAT TCAGGGCGTA 
TCTGGGGTGA AGCCCACAGG GCTCAGAAAA CGAACCTCCA GTATCGCAGA TGAGGGCACC 
TACACACTGG ACTCCATCCT TCGGCAGCTC AACTCCTTCC ACTCAGTCAT GTGTCAGCAT 
GGCATGGACC CGGAGCTAAT CAAGCAGGTA GTCAAACAGA TGTTCTACAT TGTGGGCGCC 
ATCACCCTAA ACAACCTCCT CCTGCGCAAG GACATGTGCT CCTGGAGCAA GGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTGGAAGAA TGGCTACGTG ACAAGAATCT AATGAACAGT 
GGGGCCAAGG AGACTCTGGA ACCTCTTATC CAGGCCGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACTGACG ATGACGCAGA AGCCATCTGT TCCATGTGCA ACGCCCTGAC CACGGCCCAG 
ATTGTCAAAG TGTTGAATCT GTACACACCG GTTAATGAGT TTGAAGAAAG GGTCTCAGTT 
TCATTTATCC GCACTATACA GATGCGGTTA CGAGACAGGA AAGACTCTCC ACAGCTGCTC 
ATGGATGCAA AACACATCTT TCCTGTCACT TTTCCCTTTA ACCCATCCTC CCTAGCCCTA 
GAAACCATCC AGATCCCAGC CAGCCTGGGC CTGGGCTTCA TCGCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_006510829.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510892.1 Mus musculus myosin VA (Myo5a), transcript variant X3, mRNA.

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CloneID	OMu42556
Clone ID Related Accession (Same CDS sequence)	XM_006510829.3 , XM_006510829.4
Accession Version	XM_006510829.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510829.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGA 
TCATCTACTA TGGATTACCA GGAGTTGAAT GAGGATGGAG AGCTCTGGAT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCTTAGAA TCCCAGCTAC AGTCACAGAA AAGAAGCCAT 
GAGAATGAGG CTGAGGCCCT CCGTGGGGAG ATCCAGAGCC TAAAGGAAGA AAACAACCGG 
CAACAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCATG AGATCACCCG GCTGACCAAT GAAAACCTGG ATCTGATGGA ACAACTTGAA 
AAGCAGGATA AAACTGTCCG GAAACTGAAG AAACAACTGA AAGTCTTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGGCA GATGGAGAAC ATATCTCCAG GACAGATCAT CGATGAGCCT 
ATCCGGCCAG TCAACATTCC CCGGAAAGAA AAGGATTTCC AAGGAATGCT GGAGTACAAA 
AGGGAGGATG AGCAGAAGCT AGTGAAGAAC CTGATTCTAG AACTAAAGCC ACGTGGTGTG 
GCTGTCAATC TGATTCCAGG GTTACCAGCA TATATCTTGT TTATGTGTGT ACGACATGCT 
GACTATCTGA ACGATGATCA GAAAGTAAGA TCATTGCTGA CATCAACAAT TAACAGCATC 
AAAAAAGTCT TGAAGAAAAG AGGTGACGAT TTTGAAACTG TCTCCTTCTG GCTCTCTAAC 
ACATGTCGAT TTTTGCACTG TTTGAAGCAA TATAGTGGAG AGGAGGGCTT TATGAAGCAC 
AACACGTCTC GCCAGAATGA GCACTGCCTC ACCAATTTTG ACCTTGCTGA ATATCGGCAA 
GTACTGAGTG ACTTGGCCAT TCAGATCTAT CAGCAGCTTG TGAGGGTGTT AGAGAACATT 
CTTCAGCCAA TGATAGTCTC AGGTATGCTA GAGCATGAAA CAATTCAGGG CGTATCTGGG 
GTGAAGCCCA CAGGGCTCAG AAAACGAACC TCCAGTATCG CAGATGAGGG CACCTACACA 
CTGGACTCCA TCCTTCGGCA GCTCAACTCC TTCCACTCAG TCATGTGTCA GCATGGCATG 
GACCCGGAGC TAATCAAGCA GGTAGTCAAA CAGATGTTCT ACATTGTGGG CGCCATCACC 
CTAAACAACC TCCTCCTGCG CAAGGACATG TGCTCCTGGA GCAAGGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTGGA AGAATGGCTA CGTGACAAGA ATCTAATGAA CAGTGGGGCC 
AAGGAGACTC TGGAACCTCT TATCCAGGCC GCTCAGCTTT TGCAAGTGAA AAAGAAAACT 
GACGATGACG CAGAAGCCAT CTGTTCCATG TGCAACGCCC TGACCACGGC CCAGATTGTC 
AAAGTGTTGA ATCTGTACAC ACCGGTTAAT GAGTTTGAAG AAAGGGTCTC AGTTTCATTT 
ATCCGCACTA TACAGATGCG GTTACGAGAC AGGAAAGACT CTCCACAGCT GCTCATGGAT 
GCAAAACACA TCTTTCCTGT CACTTTTCCC TTTAACCCAT CCTCCCTAGC CCTAGAAACC 
ATCCAGATCC CAGCCAGCCT GGGCCTGGGC TTCATCGCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510892.1
CDS	1835..7402
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSTMDYQELNEDGELWMVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510829.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510829.4

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGA 
TCATCTACTA TGGATTACCA GGAGTTGAAT GAGGATGGAG AGCTCTGGAT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCTTAGAA TCCCAGCTAC AGTCACAGAA AAGAAGCCAT 
GAGAATGAGG CTGAGGCCCT CCGTGGGGAG ATCCAGAGCC TAAAGGAAGA AAACAACCGG 
CAACAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCATG AGATCACCCG GCTGACCAAT GAAAACCTGG ATCTGATGGA ACAACTTGAA 
AAGCAGGATA AAACTGTCCG GAAACTGAAG AAACAACTGA AAGTCTTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGGCA GATGGAGAAC ATATCTCCAG GACAGATCAT CGATGAGCCT 
ATCCGGCCAG TCAACATTCC CCGGAAAGAA AAGGATTTCC AAGGAATGCT GGAGTACAAA 
AGGGAGGATG AGCAGAAGCT AGTGAAGAAC CTGATTCTAG AACTAAAGCC ACGTGGTGTG 
GCTGTCAATC TGATTCCAGG GTTACCAGCA TATATCTTGT TTATGTGTGT ACGACATGCT 
GACTATCTGA ACGATGATCA GAAAGTAAGA TCATTGCTGA CATCAACAAT TAACAGCATC 
AAAAAAGTCT TGAAGAAAAG AGGTGACGAT TTTGAAACTG TCTCCTTCTG GCTCTCTAAC 
ACATGTCGAT TTTTGCACTG TTTGAAGCAA TATAGTGGAG AGGAGGGCTT TATGAAGCAC 
AACACGTCTC GCCAGAATGA GCACTGCCTC ACCAATTTTG ACCTTGCTGA ATATCGGCAA 
GTACTGAGTG ACTTGGCCAT TCAGATCTAT CAGCAGCTTG TGAGGGTGTT AGAGAACATT 
CTTCAGCCAA TGATAGTCTC AGGTATGCTA GAGCATGAAA CAATTCAGGG CGTATCTGGG 
GTGAAGCCCA CAGGGCTCAG AAAACGAACC TCCAGTATCG CAGATGAGGG CACCTACACA 
CTGGACTCCA TCCTTCGGCA GCTCAACTCC TTCCACTCAG TCATGTGTCA GCATGGCATG 
GACCCGGAGC TAATCAAGCA GGTAGTCAAA CAGATGTTCT ACATTGTGGG CGCCATCACC 
CTAAACAACC TCCTCCTGCG CAAGGACATG TGCTCCTGGA GCAAGGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTGGA AGAATGGCTA CGTGACAAGA ATCTAATGAA CAGTGGGGCC 
AAGGAGACTC TGGAACCTCT TATCCAGGCC GCTCAGCTTT TGCAAGTGAA AAAGAAAACT 
GACGATGACG CAGAAGCCAT CTGTTCCATG TGCAACGCCC TGACCACGGC CCAGATTGTC 
AAAGTGTTGA ATCTGTACAC ACCGGTTAAT GAGTTTGAAG AAAGGGTCTC AGTTTCATTT 
ATCCGCACTA TACAGATGCG GTTACGAGAC AGGAAAGACT CTCCACAGCT GCTCATGGAT 
GCAAAACACA TCTTTCCTGT CACTTTTCCC TTTAACCCAT CCTCCCTAGC CCTAGAAACC 
ATCCAGATCC CAGCCAGCCT GGGCCTGGGC TTCATCGCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_006510830.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510893.1 Mus musculus myosin VA (Myo5a), transcript variant X4, mRNA.

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CloneID	OMu42557
Clone ID Related Accession (Same CDS sequence)	XM_006510830.4 , XM_006510830.3
Accession Version	XM_006510830.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510830.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG ATCATCTACT 
ATGGATTACC AGGAGTTGAA TGAGGATGGA GAGCTCTGGA TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCTTAGA ATCCCAGCTA CAGTCACAGA AAAGAAGCCA TGAGAATGAG 
GCTGAGGCCC TCCGTGGGGA GATCCAGAGC CTAAAGGAAG AAAACAACCG GCAACAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCT GAGGCCCGCA TTGAGGCCAG CCTGCAGCAT 
GAGATCACCC GGCTGACCAA TGAAAACCTG GATCTGATGG AACAACTTGA AAAGCAGGAT 
AAAACTGTCC GGAAACTGAA GAAACAACTG AAAGTCTTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGGC AGATGGAGAA CATATCTCCA GGACAGATCA TCGATGAGCC TATCCGGCCA 
GTCAACATTC CCCGGAAAGA AAAGGATTTC CAAGGAATGC TGGAGTACAA AAGGGAGGAT 
GAGCAGAAGC TAGTGAAGAA CCTGATTCTA GAACTAAAGC CACGTGGTGT GGCTGTCAAT 
CTGATTCCAG GGTTACCAGC ATATATCTTG TTTATGTGTG TACGACATGC TGACTATCTG 
AACGATGATC AGAAAGTAAG ATCATTGCTG ACATCAACAA TTAACAGCAT CAAAAAAGTC 
TTGAAGAAAA GAGGTGACGA TTTTGAAACT GTCTCCTTCT GGCTCTCTAA CACATGTCGA 
TTTTTGCACT GTTTGAAGCA ATATAGTGGA GAGGAGGGCT TTATGAAGCA CAACACGTCT 
CGCCAGAATG AGCACTGCCT CACCAATTTT GACCTTGCTG AATATCGGCA AGTACTGAGT 
GACTTGGCCA TTCAGATCTA TCAGCAGCTT GTGAGGGTGT TAGAGAACAT TCTTCAGCCA 
ATGATAGTCT CAGGTATGCT AGAGCATGAA ACAATTCAGG GCGTATCTGG GGTGAAGCCC 
ACAGGGCTCA GAAAACGAAC CTCCAGTATC GCAGATGAGG GCACCTACAC ACTGGACTCC 
ATCCTTCGGC AGCTCAACTC CTTCCACTCA GTCATGTGTC AGCATGGCAT GGACCCGGAG 
CTAATCAAGC AGGTAGTCAA ACAGATGTTC TACATTGTGG GCGCCATCAC CCTAAACAAC 
CTCCTCCTGC GCAAGGACAT GTGCTCCTGG AGCAAGGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTGG AAGAATGGCT ACGTGACAAG AATCTAATGA ACAGTGGGGC CAAGGAGACT 
CTGGAACCTC TTATCCAGGC CGCTCAGCTT TTGCAAGTGA AAAAGAAAAC TGACGATGAC 
GCAGAAGCCA TCTGTTCCAT GTGCAACGCC CTGACCACGG CCCAGATTGT CAAAGTGTTG 
AATCTGTACA CACCGGTTAA TGAGTTTGAA GAAAGGGTCT CAGTTTCATT TATCCGCACT 
ATACAGATGC GGTTACGAGA CAGGAAAGAC TCTCCACAGC TGCTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACTTTTCC CTTTAACCCA TCCTCCCTAG CCCTAGAAAC CATCCAGATC 
CCAGCCAGCC TGGGCCTGGG CTTCATCGCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510893.1
CDS	1835..7393
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSTMDYQELNEDGELWMVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510830.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510830.4

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG ATCATCTACT 
ATGGATTACC AGGAGTTGAA TGAGGATGGA GAGCTCTGGA TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCTTAGA ATCCCAGCTA CAGTCACAGA AAAGAAGCCA TGAGAATGAG 
GCTGAGGCCC TCCGTGGGGA GATCCAGAGC CTAAAGGAAG AAAACAACCG GCAACAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCT GAGGCCCGCA TTGAGGCCAG CCTGCAGCAT 
GAGATCACCC GGCTGACCAA TGAAAACCTG GATCTGATGG AACAACTTGA AAAGCAGGAT 
AAAACTGTCC GGAAACTGAA GAAACAACTG AAAGTCTTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGGC AGATGGAGAA CATATCTCCA GGACAGATCA TCGATGAGCC TATCCGGCCA 
GTCAACATTC CCCGGAAAGA AAAGGATTTC CAAGGAATGC TGGAGTACAA AAGGGAGGAT 
GAGCAGAAGC TAGTGAAGAA CCTGATTCTA GAACTAAAGC CACGTGGTGT GGCTGTCAAT 
CTGATTCCAG GGTTACCAGC ATATATCTTG TTTATGTGTG TACGACATGC TGACTATCTG 
AACGATGATC AGAAAGTAAG ATCATTGCTG ACATCAACAA TTAACAGCAT CAAAAAAGTC 
TTGAAGAAAA GAGGTGACGA TTTTGAAACT GTCTCCTTCT GGCTCTCTAA CACATGTCGA 
TTTTTGCACT GTTTGAAGCA ATATAGTGGA GAGGAGGGCT TTATGAAGCA CAACACGTCT 
CGCCAGAATG AGCACTGCCT CACCAATTTT GACCTTGCTG AATATCGGCA AGTACTGAGT 
GACTTGGCCA TTCAGATCTA TCAGCAGCTT GTGAGGGTGT TAGAGAACAT TCTTCAGCCA 
ATGATAGTCT CAGGTATGCT AGAGCATGAA ACAATTCAGG GCGTATCTGG GGTGAAGCCC 
ACAGGGCTCA GAAAACGAAC CTCCAGTATC GCAGATGAGG GCACCTACAC ACTGGACTCC 
ATCCTTCGGC AGCTCAACTC CTTCCACTCA GTCATGTGTC AGCATGGCAT GGACCCGGAG 
CTAATCAAGC AGGTAGTCAA ACAGATGTTC TACATTGTGG GCGCCATCAC CCTAAACAAC 
CTCCTCCTGC GCAAGGACAT GTGCTCCTGG AGCAAGGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTGG AAGAATGGCT ACGTGACAAG AATCTAATGA ACAGTGGGGC CAAGGAGACT 
CTGGAACCTC TTATCCAGGC CGCTCAGCTT TTGCAAGTGA AAAAGAAAAC TGACGATGAC 
GCAGAAGCCA TCTGTTCCAT GTGCAACGCC CTGACCACGG CCCAGATTGT CAAAGTGTTG 
AATCTGTACA CACCGGTTAA TGAGTTTGAA GAAAGGGTCT CAGTTTCATT TATCCGCACT 
ATACAGATGC GGTTACGAGA CAGGAAAGAC TCTCCACAGC TGCTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACTTTTCC CTTTAACCCA TCCTCCCTAG CCCTAGAAAC CATCCAGATC 
CCAGCCAGCC TGGGCCTGGG CTTCATCGCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_006510831.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510894.1 Mus musculus myosin VA (Myo5a), transcript variant X5, mRNA.

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CloneID	OMu42558
Clone ID Related Accession (Same CDS sequence)	XM_006510831.3 , XM_006510831.4
Accession Version	XM_006510831.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510831.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG GCTCTTAGAA 
TCCCAGCTAC AGTCACAGAA AAGAAGCCAT GAGAATGAGG CTGAGGCCCT CCGTGGGGAG 
ATCCAGAGCC TAAAGGAAGA AAACAACCGG CAACAGCAGC TGCTGGCCCA GAACCTGCAG 
CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC CTGCAGCATG AGATCACCCG GCTGACCAAT 
GAAAACCTGT ATTTTGAGGA ATTATATGCA GATGACCCTA AGAAGTATCA ATCCTATCGG 
ATTTCACTTT ACAAAAGGAT GATTGATCTG ATGGAACAAC TTGAAAAGCA GGATAAAACT 
GTCCGGAAAC TGAAGAAACA ACTGAAAGTC TTTGCCAAAA AAATTGGTGA ACTAGAAGTG 
GGGCAGATGG AGAACATATC TCCAGGACAG ATCATCGATG AGCCTATCCG GCCAGTCAAC 
ATTCCCCGGA AAGAAAAGGA TTTCCAAGGA ATGCTGGAGT ACAAAAGGGA GGATGAGCAG 
AAGCTAGTGA AGAACCTGAT TCTAGAACTA AAGCCACGTG GTGTGGCTGT CAATCTGATT 
CCAGGGTTAC CAGCATATAT CTTGTTTATG TGTGTACGAC ATGCTGACTA TCTGAACGAT 
GATCAGAAAG TAAGATCATT GCTGACATCA ACAATTAACA GCATCAAAAA AGTCTTGAAG 
AAAAGAGGTG ACGATTTTGA AACTGTCTCC TTCTGGCTCT CTAACACATG TCGATTTTTG 
CACTGTTTGA AGCAATATAG TGGAGAGGAG GGCTTTATGA AGCACAACAC GTCTCGCCAG 
AATGAGCACT GCCTCACCAA TTTTGACCTT GCTGAATATC GGCAAGTACT GAGTGACTTG 
GCCATTCAGA TCTATCAGCA GCTTGTGAGG GTGTTAGAGA ACATTCTTCA GCCAATGATA 
GTCTCAGGTA TGCTAGAGCA TGAAACAATT CAGGGCGTAT CTGGGGTGAA GCCCACAGGG 
CTCAGAAAAC GAACCTCCAG TATCGCAGAT GAGGGCACCT ACACACTGGA CTCCATCCTT 
CGGCAGCTCA ACTCCTTCCA CTCAGTCATG TGTCAGCATG GCATGGACCC GGAGCTAATC 
AAGCAGGTAG TCAAACAGAT GTTCTACATT GTGGGCGCCA TCACCCTAAA CAACCTCCTC 
CTGCGCAAGG ACATGTGCTC CTGGAGCAAG GGCATGCAGA TCAGGTACAA TGTCAGTCAA 
CTGGAAGAAT GGCTACGTGA CAAGAATCTA ATGAACAGTG GGGCCAAGGA GACTCTGGAA 
CCTCTTATCC AGGCCGCTCA GCTTTTGCAA GTGAAAAAGA AAACTGACGA TGACGCAGAA 
GCCATCTGTT CCATGTGCAA CGCCCTGACC ACGGCCCAGA TTGTCAAAGT GTTGAATCTG 
TACACACCGG TTAATGAGTT TGAAGAAAGG GTCTCAGTTT CATTTATCCG CACTATACAG 
ATGCGGTTAC GAGACAGGAA AGACTCTCCA CAGCTGCTCA TGGATGCAAA ACACATCTTT 
CCTGTCACTT TTCCCTTTAA CCCATCCTCC CTAGCCCTAG AAACCATCCA GATCCCAGCC 
AGCCTGGGCC TGGGCTTCAT CGCACGGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510894.1
CDS	1835..7387
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510831.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510831.4

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG GCTCTTAGAA 
TCCCAGCTAC AGTCACAGAA AAGAAGCCAT GAGAATGAGG CTGAGGCCCT CCGTGGGGAG 
ATCCAGAGCC TAAAGGAAGA AAACAACCGG CAACAGCAGC TGCTGGCCCA GAACCTGCAG 
CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC CTGCAGCATG AGATCACCCG GCTGACCAAT 
GAAAACCTGT ATTTTGAGGA ATTATATGCA GATGACCCTA AGAAGTATCA ATCCTATCGG 
ATTTCACTTT ACAAAAGGAT GATTGATCTG ATGGAACAAC TTGAAAAGCA GGATAAAACT 
GTCCGGAAAC TGAAGAAACA ACTGAAAGTC TTTGCCAAAA AAATTGGTGA ACTAGAAGTG 
GGGCAGATGG AGAACATATC TCCAGGACAG ATCATCGATG AGCCTATCCG GCCAGTCAAC 
ATTCCCCGGA AAGAAAAGGA TTTCCAAGGA ATGCTGGAGT ACAAAAGGGA GGATGAGCAG 
AAGCTAGTGA AGAACCTGAT TCTAGAACTA AAGCCACGTG GTGTGGCTGT CAATCTGATT 
CCAGGGTTAC CAGCATATAT CTTGTTTATG TGTGTACGAC ATGCTGACTA TCTGAACGAT 
GATCAGAAAG TAAGATCATT GCTGACATCA ACAATTAACA GCATCAAAAA AGTCTTGAAG 
AAAAGAGGTG ACGATTTTGA AACTGTCTCC TTCTGGCTCT CTAACACATG TCGATTTTTG 
CACTGTTTGA AGCAATATAG TGGAGAGGAG GGCTTTATGA AGCACAACAC GTCTCGCCAG 
AATGAGCACT GCCTCACCAA TTTTGACCTT GCTGAATATC GGCAAGTACT GAGTGACTTG 
GCCATTCAGA TCTATCAGCA GCTTGTGAGG GTGTTAGAGA ACATTCTTCA GCCAATGATA 
GTCTCAGGTA TGCTAGAGCA TGAAACAATT CAGGGCGTAT CTGGGGTGAA GCCCACAGGG 
CTCAGAAAAC GAACCTCCAG TATCGCAGAT GAGGGCACCT ACACACTGGA CTCCATCCTT 
CGGCAGCTCA ACTCCTTCCA CTCAGTCATG TGTCAGCATG GCATGGACCC GGAGCTAATC 
AAGCAGGTAG TCAAACAGAT GTTCTACATT GTGGGCGCCA TCACCCTAAA CAACCTCCTC 
CTGCGCAAGG ACATGTGCTC CTGGAGCAAG GGCATGCAGA TCAGGTACAA TGTCAGTCAA 
CTGGAAGAAT GGCTACGTGA CAAGAATCTA ATGAACAGTG GGGCCAAGGA GACTCTGGAA 
CCTCTTATCC AGGCCGCTCA GCTTTTGCAA GTGAAAAAGA AAACTGACGA TGACGCAGAA 
GCCATCTGTT CCATGTGCAA CGCCCTGACC ACGGCCCAGA TTGTCAAAGT GTTGAATCTG 
TACACACCGG TTAATGAGTT TGAAGAAAGG GTCTCAGTTT CATTTATCCG CACTATACAG 
ATGCGGTTAC GAGACAGGAA AGACTCTCCA CAGCTGCTCA TGGATGCAAA ACACATCTTT 
CCTGTCACTT TTCCCTTTAA CCCATCCTCC CTAGCCCTAG AAACCATCCA GATCCCAGCC 
AGCCTGGGCC TGGGCTTCAT CGCACGGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_006510832.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510895.1 Mus musculus myosin VA (Myo5a), transcript variant X6, mRNA.

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CloneID	OMu42559
Clone ID Related Accession (Same CDS sequence)	XM_006510832.4 , XM_006510832.3
Accession Version	XM_006510832.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510832.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGG 
CTCTTAGAAT CCCAGCTACA GTCACAGAAA AGAAGCCATG AGAATGAGGC TGAGGCCCTC 
CGTGGGGAGA TCCAGAGCCT AAAGGAAGAA AACAACCGGC AACAGCAGCT GCTGGCCCAG 
AACCTGCAGC TGCCCCCTGA GGCCCGCATT GAGGCCAGCC TGCAGCATGA GATCACCCGG 
CTGACCAATG AAAACCTGGA TCTGATGGAA CAACTTGAAA AGCAGGATAA AACTGTCCGG 
AAACTGAAGA AACAACTGAA AGTCTTTGCC AAAAAAATTG GTGAACTAGA AGTGGGGCAG 
ATGGAGAACA TATCTCCAGG ACAGATCATC GATGAGCCTA TCCGGCCAGT CAACATTCCC 
CGGAAAGAAA AGGATTTCCA AGGAATGCTG GAGTACAAAA GGGAGGATGA GCAGAAGCTA 
GTGAAGAACC TGATTCTAGA ACTAAAGCCA CGTGGTGTGG CTGTCAATCT GATTCCAGGG 
TTACCAGCAT ATATCTTGTT TATGTGTGTA CGACATGCTG ACTATCTGAA CGATGATCAG 
AAAGTAAGAT CATTGCTGAC ATCAACAATT AACAGCATCA AAAAAGTCTT GAAGAAAAGA 
GGTGACGATT TTGAAACTGT CTCCTTCTGG CTCTCTAACA CATGTCGATT TTTGCACTGT 
TTGAAGCAAT ATAGTGGAGA GGAGGGCTTT ATGAAGCACA ACACGTCTCG CCAGAATGAG 
CACTGCCTCA CCAATTTTGA CCTTGCTGAA TATCGGCAAG TACTGAGTGA CTTGGCCATT 
CAGATCTATC AGCAGCTTGT GAGGGTGTTA GAGAACATTC TTCAGCCAAT GATAGTCTCA 
GGTATGCTAG AGCATGAAAC AATTCAGGGC GTATCTGGGG TGAAGCCCAC AGGGCTCAGA 
AAACGAACCT CCAGTATCGC AGATGAGGGC ACCTACACAC TGGACTCCAT CCTTCGGCAG 
CTCAACTCCT TCCACTCAGT CATGTGTCAG CATGGCATGG ACCCGGAGCT AATCAAGCAG 
GTAGTCAAAC AGATGTTCTA CATTGTGGGC GCCATCACCC TAAACAACCT CCTCCTGCGC 
AAGGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ACAATGTCAG TCAACTGGAA 
GAATGGCTAC GTGACAAGAA TCTAATGAAC AGTGGGGCCA AGGAGACTCT GGAACCTCTT 
ATCCAGGCCG CTCAGCTTTT GCAAGTGAAA AAGAAAACTG ACGATGACGC AGAAGCCATC 
TGTTCCATGT GCAACGCCCT GACCACGGCC CAGATTGTCA AAGTGTTGAA TCTGTACACA 
CCGGTTAATG AGTTTGAAGA AAGGGTCTCA GTTTCATTTA TCCGCACTAT ACAGATGCGG 
TTACGAGACA GGAAAGACTC TCCACAGCTG CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACTTTTCCCT TTAACCCATC CTCCCTAGCC CTAGAAACCA TCCAGATCCC AGCCAGCCTG 
GGCCTGGGCT TCATCGCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510895.1
CDS	1835..7321
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510832.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510832.4

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGG 
CTCTTAGAAT CCCAGCTACA GTCACAGAAA AGAAGCCATG AGAATGAGGC TGAGGCCCTC 
CGTGGGGAGA TCCAGAGCCT AAAGGAAGAA AACAACCGGC AACAGCAGCT GCTGGCCCAG 
AACCTGCAGC TGCCCCCTGA GGCCCGCATT GAGGCCAGCC TGCAGCATGA GATCACCCGG 
CTGACCAATG AAAACCTGGA TCTGATGGAA CAACTTGAAA AGCAGGATAA AACTGTCCGG 
AAACTGAAGA AACAACTGAA AGTCTTTGCC AAAAAAATTG GTGAACTAGA AGTGGGGCAG 
ATGGAGAACA TATCTCCAGG ACAGATCATC GATGAGCCTA TCCGGCCAGT CAACATTCCC 
CGGAAAGAAA AGGATTTCCA AGGAATGCTG GAGTACAAAA GGGAGGATGA GCAGAAGCTA 
GTGAAGAACC TGATTCTAGA ACTAAAGCCA CGTGGTGTGG CTGTCAATCT GATTCCAGGG 
TTACCAGCAT ATATCTTGTT TATGTGTGTA CGACATGCTG ACTATCTGAA CGATGATCAG 
AAAGTAAGAT CATTGCTGAC ATCAACAATT AACAGCATCA AAAAAGTCTT GAAGAAAAGA 
GGTGACGATT TTGAAACTGT CTCCTTCTGG CTCTCTAACA CATGTCGATT TTTGCACTGT 
TTGAAGCAAT ATAGTGGAGA GGAGGGCTTT ATGAAGCACA ACACGTCTCG CCAGAATGAG 
CACTGCCTCA CCAATTTTGA CCTTGCTGAA TATCGGCAAG TACTGAGTGA CTTGGCCATT 
CAGATCTATC AGCAGCTTGT GAGGGTGTTA GAGAACATTC TTCAGCCAAT GATAGTCTCA 
GGTATGCTAG AGCATGAAAC AATTCAGGGC GTATCTGGGG TGAAGCCCAC AGGGCTCAGA 
AAACGAACCT CCAGTATCGC AGATGAGGGC ACCTACACAC TGGACTCCAT CCTTCGGCAG 
CTCAACTCCT TCCACTCAGT CATGTGTCAG CATGGCATGG ACCCGGAGCT AATCAAGCAG 
GTAGTCAAAC AGATGTTCTA CATTGTGGGC GCCATCACCC TAAACAACCT CCTCCTGCGC 
AAGGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ACAATGTCAG TCAACTGGAA 
GAATGGCTAC GTGACAAGAA TCTAATGAAC AGTGGGGCCA AGGAGACTCT GGAACCTCTT 
ATCCAGGCCG CTCAGCTTTT GCAAGTGAAA AAGAAAACTG ACGATGACGC AGAAGCCATC 
TGTTCCATGT GCAACGCCCT GACCACGGCC CAGATTGTCA AAGTGTTGAA TCTGTACACA 
CCGGTTAATG AGTTTGAAGA AAGGGTCTCA GTTTCATTTA TCCGCACTAT ACAGATGCGG 
TTACGAGACA GGAAAGACTC TCCACAGCTG CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACTTTTCCCT TTAACCCATC CTCCCTAGCC CTAGAAACCA TCCAGATCCC AGCCAGCCTG 
GGCCTGGGCT TCATCGCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_030244103.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099963.1 Mus musculus myosin VA (Myo5a), transcript variant X7, mRNA.

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CloneID	OMu90090
Clone ID Related Accession (Same CDS sequence)	XM_030244103.1
Accession Version	XM_030244103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG GCTCTTAGAA 
TCCCAGCTAC AGTCACAGAA AAGAAGCCAT GAGAATGAGG CTGAGGCCCT CCGTGGGGAG 
ATCCAGAGCC TAAAGGAAGA AAACAACCGG CAACAGCAGC TGCTGGCCCA GAACCTGCAG 
CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC CTGCAGCATG AGATCACCCG GCTGACCAAT 
GAAAACCTGG ATCTGATGGA ACAACTTGAA AAGCAGGATA AAACTGTCCG GAAACTGAAG 
AAACAACTGA AAGTCTTTGC CAAAAAAATT GGTGAACTAG AAGTGGGGCA GATGGAGAAC 
ATATCTCCAG GACAGATCAT CGATGAGCCT ATCCGGCCAG TCAACATTCC CCGGAAAGAA 
AAGGATTTCC AAGGAATGCT GGAGTACAAA AGGGAGGATG AGCAGAAGCT AGTGAAGAAC 
CTGATTCTAG AACTAAAGCC ACGTGGTGTG GCTGTCAATC TGATTCCAGG GTTACCAGCA 
TATATCTTGT TTATGTGTGT ACGACATGCT GACTATCTGA ACGATGATCA GAAAGTAAGA 
TCATTGCTGA CATCAACAAT TAACAGCATC AAAAAAGTCT TGAAGAAAAG AGGTGACGAT 
TTTGAAACTG TCTCCTTCTG GCTCTCTAAC ACATGTCGAT TTTTGCACTG TTTGAAGCAA 
TATAGTGGAG AGGAGGGCTT TATGAAGCAC AACACGTCTC GCCAGAATGA GCACTGCCTC 
ACCAATTTTG ACCTTGCTGA ATATCGGCAA GTACTGAGTG ACTTGGCCAT TCAGATCTAT 
CAGCAGCTTG TGAGGGTGTT AGAGAACATT CTTCAGCCAA TGATAGTCTC AGGTATGCTA 
GAGCATGAAA CAATTCAGGG CGTATCTGGG GTGAAGCCCA CAGGGCTCAG AAAACGAACC 
TCCAGTATCG CAGATGAGGG CACCTACACA CTGGACTCCA TCCTTCGGCA GCTCAACTCC 
TTCCACTCAG TCATGTGTCA GCATGGCATG GACCCGGAGC TAATCAAGCA GGTAGTCAAA 
CAGATGTTCT ACATTGTGGG CGCCATCACC CTAAACAACC TCCTCCTGCG CAAGGACATG 
TGCTCCTGGA GCAAGGGCAT GCAGATCAGG TACAATGTCA GTCAACTGGA AGAATGGCTA 
CGTGACAAGA ATCTAATGAA CAGTGGGGCC AAGGAGACTC TGGAACCTCT TATCCAGGCC 
GCTCAGCTTT TGCAAGTGAA AAAGAAAACT GACGATGACG CAGAAGCCAT CTGTTCCATG 
TGCAACGCCC TGACCACGGC CCAGATTGTC AAAGTGTTGA ATCTGTACAC ACCGGTTAAT 
GAGTTTGAAG AAAGGGTCTC AGTTTCATTT ATCCGCACTA TACAGATGCG GTTACGAGAC 
AGGAAAGACT CTCCACAGCT GCTCATGGAT GCAAAACACA TCTTTCCTGT CACTTTTCCC 
TTTAACCCAT CCTCCCTAGC CCTAGAAACC ATCCAGATCC CAGCCAGCCT GGGCCTGGGC 
TTCATCGCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099963.1
CDS	1835..7312
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_030244103.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244103.1

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG GCTCTTAGAA 
TCCCAGCTAC AGTCACAGAA AAGAAGCCAT GAGAATGAGG CTGAGGCCCT CCGTGGGGAG 
ATCCAGAGCC TAAAGGAAGA AAACAACCGG CAACAGCAGC TGCTGGCCCA GAACCTGCAG 
CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC CTGCAGCATG AGATCACCCG GCTGACCAAT 
GAAAACCTGG ATCTGATGGA ACAACTTGAA AAGCAGGATA AAACTGTCCG GAAACTGAAG 
AAACAACTGA AAGTCTTTGC CAAAAAAATT GGTGAACTAG AAGTGGGGCA GATGGAGAAC 
ATATCTCCAG GACAGATCAT CGATGAGCCT ATCCGGCCAG TCAACATTCC CCGGAAAGAA 
AAGGATTTCC AAGGAATGCT GGAGTACAAA AGGGAGGATG AGCAGAAGCT AGTGAAGAAC 
CTGATTCTAG AACTAAAGCC ACGTGGTGTG GCTGTCAATC TGATTCCAGG GTTACCAGCA 
TATATCTTGT TTATGTGTGT ACGACATGCT GACTATCTGA ACGATGATCA GAAAGTAAGA 
TCATTGCTGA CATCAACAAT TAACAGCATC AAAAAAGTCT TGAAGAAAAG AGGTGACGAT 
TTTGAAACTG TCTCCTTCTG GCTCTCTAAC ACATGTCGAT TTTTGCACTG TTTGAAGCAA 
TATAGTGGAG AGGAGGGCTT TATGAAGCAC AACACGTCTC GCCAGAATGA GCACTGCCTC 
ACCAATTTTG ACCTTGCTGA ATATCGGCAA GTACTGAGTG ACTTGGCCAT TCAGATCTAT 
CAGCAGCTTG TGAGGGTGTT AGAGAACATT CTTCAGCCAA TGATAGTCTC AGGTATGCTA 
GAGCATGAAA CAATTCAGGG CGTATCTGGG GTGAAGCCCA CAGGGCTCAG AAAACGAACC 
TCCAGTATCG CAGATGAGGG CACCTACACA CTGGACTCCA TCCTTCGGCA GCTCAACTCC 
TTCCACTCAG TCATGTGTCA GCATGGCATG GACCCGGAGC TAATCAAGCA GGTAGTCAAA 
CAGATGTTCT ACATTGTGGG CGCCATCACC CTAAACAACC TCCTCCTGCG CAAGGACATG 
TGCTCCTGGA GCAAGGGCAT GCAGATCAGG TACAATGTCA GTCAACTGGA AGAATGGCTA 
CGTGACAAGA ATCTAATGAA CAGTGGGGCC AAGGAGACTC TGGAACCTCT TATCCAGGCC 
GCTCAGCTTT TGCAAGTGAA AAAGAAAACT GACGATGACG CAGAAGCCAT CTGTTCCATG 
TGCAACGCCC TGACCACGGC CCAGATTGTC AAAGTGTTGA ATCTGTACAC ACCGGTTAAT 
GAGTTTGAAG AAAGGGTCTC AGTTTCATTT ATCCGCACTA TACAGATGCG GTTACGAGAC 
AGGAAAGACT CTCCACAGCT GCTCATGGAT GCAAAACACA TCTTTCCTGT CACTTTTCCC 
TTTAACCCAT CCTCCCTAGC CCTAGAAACC ATCCAGATCC CAGCCAGCCT GGGCCTGGGC 
TTCATCGCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( NM_010864.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034994.2 Mus musculus myosin VA (Myo5a), mRNA.

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CloneID	OMu22050
Clone ID Related Accession (Same CDS sequence)	NM_010864.2
Accession Version	NM_010864.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK134367.1, X57377.1, AK149701.1, CN459715.1, AK002362.1 and AC133947.3. On Oct 4, 2006 this sequence version replaced NM_010864.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X57377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGG 
CTCTTAGAAT CCCAGCTACA GTCACAGAAA AGAAGCCATG AGAATGAGGC TGAGGCCCTC 
CGTGGGGAGA TCCAGAGCCT AAAGGAAGAA AACAACCGGC AACAGCAGCT GCTGGCCCAG 
AACCTGCAGC TGCCCCCTGA GGCCCGCATT GAGGCCAGCC TGCAGCATGA GATCACCCGG 
CTGACCAATG AAAACCTGTA TTTTGAGGAA TTATATGCAG ATGACCCTAA GAAGTATCAA 
TCCTATCGGA TTTCACTTTA CAAAAGGATG ATTGATCTGA TGGAACAACT TGAAAAGCAG 
GATAAAACTG TCCGGAAACT GAAGAAACAA CTGAAAGTCT TTGCCAAAAA AATTGGTGAA 
CTAGAAGTGG GGCAGATGGA GAACATATCT CCAGGACAGA TCATCGATGA GCCTATCCGG 
CCAGTCAACA TTCCCCGGAA AGAAAAGGAT TTCCAAGGAA TGCTGGAGTA CAAAAGGGAG 
GATGAGCAGA AGCTAGTGAA GAACCTGATT CTAGAACTAA AGCCACGTGG TGTGGCTGTC 
AATCTGATTC CAGGGTTACC AGCATATATC TTGTTTATGT GTGTACGACA TGCTGACTAT 
CTGAACGATG ATCAGAAAGT AAGATCATTG CTGACATCAA CAATTAACAG CATCAAAAAA 
GTCTTGAAGA AAAGAGGTGA CGATTTTGAA ACTGTCTCCT TCTGGCTCTC TAACACATGT 
CGATTTTTGC ACTGTTTGAA GCAATATAGT GGAGAGGAGG GCTTTATGAA GCACAACACG 
TCTCGCCAGA ATGAGCACTG CCTCACCAAT TTTGACCTTG CTGAATATCG GCAAGTACTG 
AGTGACTTGG CCATTCAGAT CTATCAGCAG CTTGTGAGGG TGTTAGAGAA CATTCTTCAG 
CCAATGATAG TCTCAGGTAT GCTAGAGCAT GAAACAATTC AGGGCGTATC TGGGGTGAAG 
CCCACAGGGC TCAGAAAACG AACCTCCAGT ATCGCAGATG AGGGCACCTA CACACTGGAC 
TCCATCCTTC GGCAGCTCAA CTCCTTCCAC TCAGTCATGT GTCAGCATGG CATGGACCCG 
GAGCTAATCA AGCAGGTAGT CAAACAGATG TTCTACATTG TGGGCGCCAT CACCCTAAAC 
AACCTCCTCC TGCGCAAGGA CATGTGCTCC TGGAGCAAGG GCATGCAGAT CAGGTACAAT 
GTCAGTCAAC TGGAAGAATG GCTACGTGAC AAGAATCTAA TGAACAGTGG GGCCAAGGAG 
ACTCTGGAAC CTCTTATCCA GGCCGCTCAG CTTTTGCAAG TGAAAAAGAA AACTGACGAT 
GACGCAGAAG CCATCTGTTC CATGTGCAAC GCCCTGACCA CGGCCCAGAT TGTCAAAGTG 
TTGAATCTGT ACACACCGGT TAATGAGTTT GAAGAAAGGG TCTCAGTTTC ATTTATCCGC 
ACTATACAGA TGCGGTTACG AGACAGGAAA GACTCTCCAC AGCTGCTCAT GGATGCAAAA 
CACATCTTTC CTGTCACTTT TCCCTTTAAC CCATCCTCCC TAGCCCTAGA AACCATCCAG 
ATCCCAGCCA GCCTGGGCCT GGGCTTCATC GCACGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034994.2
CDS	428..5989
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	NM_010864.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010864.2

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGG 
CTCTTAGAAT CCCAGCTACA GTCACAGAAA AGAAGCCATG AGAATGAGGC TGAGGCCCTC 
CGTGGGGAGA TCCAGAGCCT AAAGGAAGAA AACAACCGGC AACAGCAGCT GCTGGCCCAG 
AACCTGCAGC TGCCCCCTGA GGCCCGCATT GAGGCCAGCC TGCAGCATGA GATCACCCGG 
CTGACCAATG AAAACCTGTA TTTTGAGGAA TTATATGCAG ATGACCCTAA GAAGTATCAA 
TCCTATCGGA TTTCACTTTA CAAAAGGATG ATTGATCTGA TGGAACAACT TGAAAAGCAG 
GATAAAACTG TCCGGAAACT GAAGAAACAA CTGAAAGTCT TTGCCAAAAA AATTGGTGAA 
CTAGAAGTGG GGCAGATGGA GAACATATCT CCAGGACAGA TCATCGATGA GCCTATCCGG 
CCAGTCAACA TTCCCCGGAA AGAAAAGGAT TTCCAAGGAA TGCTGGAGTA CAAAAGGGAG 
GATGAGCAGA AGCTAGTGAA GAACCTGATT CTAGAACTAA AGCCACGTGG TGTGGCTGTC 
AATCTGATTC CAGGGTTACC AGCATATATC TTGTTTATGT GTGTACGACA TGCTGACTAT 
CTGAACGATG ATCAGAAAGT AAGATCATTG CTGACATCAA CAATTAACAG CATCAAAAAA 
GTCTTGAAGA AAAGAGGTGA CGATTTTGAA ACTGTCTCCT TCTGGCTCTC TAACACATGT 
CGATTTTTGC ACTGTTTGAA GCAATATAGT GGAGAGGAGG GCTTTATGAA GCACAACACG 
TCTCGCCAGA ATGAGCACTG CCTCACCAAT TTTGACCTTG CTGAATATCG GCAAGTACTG 
AGTGACTTGG CCATTCAGAT CTATCAGCAG CTTGTGAGGG TGTTAGAGAA CATTCTTCAG 
CCAATGATAG TCTCAGGTAT GCTAGAGCAT GAAACAATTC AGGGCGTATC TGGGGTGAAG 
CCCACAGGGC TCAGAAAACG AACCTCCAGT ATCGCAGATG AGGGCACCTA CACACTGGAC 
TCCATCCTTC GGCAGCTCAA CTCCTTCCAC TCAGTCATGT GTCAGCATGG CATGGACCCG 
GAGCTAATCA AGCAGGTAGT CAAACAGATG TTCTACATTG TGGGCGCCAT CACCCTAAAC 
AACCTCCTCC TGCGCAAGGA CATGTGCTCC TGGAGCAAGG GCATGCAGAT CAGGTACAAT 
GTCAGTCAAC TGGAAGAATG GCTACGTGAC AAGAATCTAA TGAACAGTGG GGCCAAGGAG 
ACTCTGGAAC CTCTTATCCA GGCCGCTCAG CTTTTGCAAG TGAAAAAGAA AACTGACGAT 
GACGCAGAAG CCATCTGTTC CATGTGCAAC GCCCTGACCA CGGCCCAGAT TGTCAAAGTG 
TTGAATCTGT ACACACCGGT TAATGAGTTT GAAGAAAGGG TCTCAGTTTC ATTTATCCGC 
ACTATACAGA TGCGGTTACG AGACAGGAAA GACTCTCCAC AGCTGCTCAT GGATGCAAAA 
CACATCTTTC CTGTCACTTT TCCCTTTAAC CCATCCTCCC TAGCCCTAGA AACCATCCAG 
ATCCCAGCCA GCCTGGGCCT GGGCTTCATC GCACGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_006510827.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510890.1 Mus musculus myosin VA (Myo5a), transcript variant X1, mRNA.

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CloneID	OMu42554
Clone ID Related Accession (Same CDS sequence)	XM_006510827.3 , XM_006510827.4
Accession Version	XM_006510827.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510827.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGA 
TCATCTACTA TGGATTACCA GGAGTTGAAT GAGGATGGAG AGCTCTGGAT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCTTAGAA TCCCAGCTAC AGTCACAGAA AAGAAGCCAT 
GAGAATGAGG CTGAGGCCCT CCGTGGGGAG ATCCAGAGCC TAAAGGAAGA AAACAACCGG 
CAACAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCATG AGATCACCCG GCTGACCAAT GAAAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCCTATCGG ATTTCACTTT ACAAAAGGAT GATTGATCTG 
ATGGAACAAC TTGAAAAGCA GGATAAAACT GTCCGGAAAC TGAAGAAACA ACTGAAAGTC 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGGCAGATGG AGAACATATC TCCAGGACAG 
ATCATCGATG AGCCTATCCG GCCAGTCAAC ATTCCCCGGA AAGAAAAGGA TTTCCAAGGA 
ATGCTGGAGT ACAAAAGGGA GGATGAGCAG AAGCTAGTGA AGAACCTGAT TCTAGAACTA 
AAGCCACGTG GTGTGGCTGT CAATCTGATT CCAGGGTTAC CAGCATATAT CTTGTTTATG 
TGTGTACGAC ATGCTGACTA TCTGAACGAT GATCAGAAAG TAAGATCATT GCTGACATCA 
ACAATTAACA GCATCAAAAA AGTCTTGAAG AAAAGAGGTG ACGATTTTGA AACTGTCTCC 
TTCTGGCTCT CTAACACATG TCGATTTTTG CACTGTTTGA AGCAATATAG TGGAGAGGAG 
GGCTTTATGA AGCACAACAC GTCTCGCCAG AATGAGCACT GCCTCACCAA TTTTGACCTT 
GCTGAATATC GGCAAGTACT GAGTGACTTG GCCATTCAGA TCTATCAGCA GCTTGTGAGG 
GTGTTAGAGA ACATTCTTCA GCCAATGATA GTCTCAGGTA TGCTAGAGCA TGAAACAATT 
CAGGGCGTAT CTGGGGTGAA GCCCACAGGG CTCAGAAAAC GAACCTCCAG TATCGCAGAT 
GAGGGCACCT ACACACTGGA CTCCATCCTT CGGCAGCTCA ACTCCTTCCA CTCAGTCATG 
TGTCAGCATG GCATGGACCC GGAGCTAATC AAGCAGGTAG TCAAACAGAT GTTCTACATT 
GTGGGCGCCA TCACCCTAAA CAACCTCCTC CTGCGCAAGG ACATGTGCTC CTGGAGCAAG 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTGGAAGAAT GGCTACGTGA CAAGAATCTA 
ATGAACAGTG GGGCCAAGGA GACTCTGGAA CCTCTTATCC AGGCCGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACTGACGA TGACGCAGAA GCCATCTGTT CCATGTGCAA CGCCCTGACC 
ACGGCCCAGA TTGTCAAAGT GTTGAATCTG TACACACCGG TTAATGAGTT TGAAGAAAGG 
GTCTCAGTTT CATTTATCCG CACTATACAG ATGCGGTTAC GAGACAGGAA AGACTCTCCA 
CAGCTGCTCA TGGATGCAAA ACACATCTTT CCTGTCACTT TTCCCTTTAA CCCATCCTCC 
CTAGCCCTAG AAACCATCCA GATCCCAGCC AGCCTGGGCC TGGGCTTCAT CGCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510890.1
CDS	619..6261
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSTMDYQELNEDGELWMVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510827.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510827.3

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGA 
TCATCTACTA TGGATTACCA GGAGTTGAAT GAGGATGGAG AGCTCTGGAT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCTTAGAA TCCCAGCTAC AGTCACAGAA AAGAAGCCAT 
GAGAATGAGG CTGAGGCCCT CCGTGGGGAG ATCCAGAGCC TAAAGGAAGA AAACAACCGG 
CAACAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCATG AGATCACCCG GCTGACCAAT GAAAACCTGT ATTTTGAGGA ATTATATGCA 
GATGACCCTA AGAAGTATCA ATCCTATCGG ATTTCACTTT ACAAAAGGAT GATTGATCTG 
ATGGAACAAC TTGAAAAGCA GGATAAAACT GTCCGGAAAC TGAAGAAACA ACTGAAAGTC 
TTTGCCAAAA AAATTGGTGA ACTAGAAGTG GGGCAGATGG AGAACATATC TCCAGGACAG 
ATCATCGATG AGCCTATCCG GCCAGTCAAC ATTCCCCGGA AAGAAAAGGA TTTCCAAGGA 
ATGCTGGAGT ACAAAAGGGA GGATGAGCAG AAGCTAGTGA AGAACCTGAT TCTAGAACTA 
AAGCCACGTG GTGTGGCTGT CAATCTGATT CCAGGGTTAC CAGCATATAT CTTGTTTATG 
TGTGTACGAC ATGCTGACTA TCTGAACGAT GATCAGAAAG TAAGATCATT GCTGACATCA 
ACAATTAACA GCATCAAAAA AGTCTTGAAG AAAAGAGGTG ACGATTTTGA AACTGTCTCC 
TTCTGGCTCT CTAACACATG TCGATTTTTG CACTGTTTGA AGCAATATAG TGGAGAGGAG 
GGCTTTATGA AGCACAACAC GTCTCGCCAG AATGAGCACT GCCTCACCAA TTTTGACCTT 
GCTGAATATC GGCAAGTACT GAGTGACTTG GCCATTCAGA TCTATCAGCA GCTTGTGAGG 
GTGTTAGAGA ACATTCTTCA GCCAATGATA GTCTCAGGTA TGCTAGAGCA TGAAACAATT 
CAGGGCGTAT CTGGGGTGAA GCCCACAGGG CTCAGAAAAC GAACCTCCAG TATCGCAGAT 
GAGGGCACCT ACACACTGGA CTCCATCCTT CGGCAGCTCA ACTCCTTCCA CTCAGTCATG 
TGTCAGCATG GCATGGACCC GGAGCTAATC AAGCAGGTAG TCAAACAGAT GTTCTACATT 
GTGGGCGCCA TCACCCTAAA CAACCTCCTC CTGCGCAAGG ACATGTGCTC CTGGAGCAAG 
GGCATGCAGA TCAGGTACAA TGTCAGTCAA CTGGAAGAAT GGCTACGTGA CAAGAATCTA 
ATGAACAGTG GGGCCAAGGA GACTCTGGAA CCTCTTATCC AGGCCGCTCA GCTTTTGCAA 
GTGAAAAAGA AAACTGACGA TGACGCAGAA GCCATCTGTT CCATGTGCAA CGCCCTGACC 
ACGGCCCAGA TTGTCAAAGT GTTGAATCTG TACACACCGG TTAATGAGTT TGAAGAAAGG 
GTCTCAGTTT CATTTATCCG CACTATACAG ATGCGGTTAC GAGACAGGAA AGACTCTCCA 
CAGCTGCTCA TGGATGCAAA ACACATCTTT CCTGTCACTT TTCCCTTTAA CCCATCCTCC 
CTAGCCCTAG AAACCATCCA GATCCCAGCC AGCCTGGGCC TGGGCTTCAT CGCACGGGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_006510828.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510891.1 Mus musculus myosin VA (Myo5a), transcript variant X2, mRNA.

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CloneID	OMu42555
Clone ID Related Accession (Same CDS sequence)	XM_006510828.4 , XM_006510828.3
Accession Version	XM_006510828.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510828.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG ATCATCTACT 
ATGGATTACC AGGAGTTGAA TGAGGATGGA GAGCTCTGGA TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCTTAGA ATCCCAGCTA CAGTCACAGA AAAGAAGCCA TGAGAATGAG 
GCTGAGGCCC TCCGTGGGGA GATCCAGAGC CTAAAGGAAG AAAACAACCG GCAACAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCT GAGGCCCGCA TTGAGGCCAG CCTGCAGCAT 
GAGATCACCC GGCTGACCAA TGAAAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCCTATCG GATTTCACTT TACAAAAGGA TGATTGATCT GATGGAACAA 
CTTGAAAAGC AGGATAAAAC TGTCCGGAAA CTGAAGAAAC AACTGAAAGT CTTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGGCAGATG GAGAACATAT CTCCAGGACA GATCATCGAT 
GAGCCTATCC GGCCAGTCAA CATTCCCCGG AAAGAAAAGG ATTTCCAAGG AATGCTGGAG 
TACAAAAGGG AGGATGAGCA GAAGCTAGTG AAGAACCTGA TTCTAGAACT AAAGCCACGT 
GGTGTGGCTG TCAATCTGAT TCCAGGGTTA CCAGCATATA TCTTGTTTAT GTGTGTACGA 
CATGCTGACT ATCTGAACGA TGATCAGAAA GTAAGATCAT TGCTGACATC AACAATTAAC 
AGCATCAAAA AAGTCTTGAA GAAAAGAGGT GACGATTTTG AAACTGTCTC CTTCTGGCTC 
TCTAACACAT GTCGATTTTT GCACTGTTTG AAGCAATATA GTGGAGAGGA GGGCTTTATG 
AAGCACAACA CGTCTCGCCA GAATGAGCAC TGCCTCACCA ATTTTGACCT TGCTGAATAT 
CGGCAAGTAC TGAGTGACTT GGCCATTCAG ATCTATCAGC AGCTTGTGAG GGTGTTAGAG 
AACATTCTTC AGCCAATGAT AGTCTCAGGT ATGCTAGAGC ATGAAACAAT TCAGGGCGTA 
TCTGGGGTGA AGCCCACAGG GCTCAGAAAA CGAACCTCCA GTATCGCAGA TGAGGGCACC 
TACACACTGG ACTCCATCCT TCGGCAGCTC AACTCCTTCC ACTCAGTCAT GTGTCAGCAT 
GGCATGGACC CGGAGCTAAT CAAGCAGGTA GTCAAACAGA TGTTCTACAT TGTGGGCGCC 
ATCACCCTAA ACAACCTCCT CCTGCGCAAG GACATGTGCT CCTGGAGCAA GGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTGGAAGAA TGGCTACGTG ACAAGAATCT AATGAACAGT 
GGGGCCAAGG AGACTCTGGA ACCTCTTATC CAGGCCGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACTGACG ATGACGCAGA AGCCATCTGT TCCATGTGCA ACGCCCTGAC CACGGCCCAG 
ATTGTCAAAG TGTTGAATCT GTACACACCG GTTAATGAGT TTGAAGAAAG GGTCTCAGTT 
TCATTTATCC GCACTATACA GATGCGGTTA CGAGACAGGA AAGACTCTCC ACAGCTGCTC 
ATGGATGCAA AACACATCTT TCCTGTCACT TTTCCCTTTA ACCCATCCTC CCTAGCCCTA 
GAAACCATCC AGATCCCAGC CAGCCTGGGC CTGGGCTTCA TCGCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510891.1
CDS	619..6252
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSTMDYQELNEDGELWMVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510828.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510828.3

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG ATCATCTACT 
ATGGATTACC AGGAGTTGAA TGAGGATGGA GAGCTCTGGA TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCTTAGA ATCCCAGCTA CAGTCACAGA AAAGAAGCCA TGAGAATGAG 
GCTGAGGCCC TCCGTGGGGA GATCCAGAGC CTAAAGGAAG AAAACAACCG GCAACAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCT GAGGCCCGCA TTGAGGCCAG CCTGCAGCAT 
GAGATCACCC GGCTGACCAA TGAAAACCTG TATTTTGAGG AATTATATGC AGATGACCCT 
AAGAAGTATC AATCCTATCG GATTTCACTT TACAAAAGGA TGATTGATCT GATGGAACAA 
CTTGAAAAGC AGGATAAAAC TGTCCGGAAA CTGAAGAAAC AACTGAAAGT CTTTGCCAAA 
AAAATTGGTG AACTAGAAGT GGGGCAGATG GAGAACATAT CTCCAGGACA GATCATCGAT 
GAGCCTATCC GGCCAGTCAA CATTCCCCGG AAAGAAAAGG ATTTCCAAGG AATGCTGGAG 
TACAAAAGGG AGGATGAGCA GAAGCTAGTG AAGAACCTGA TTCTAGAACT AAAGCCACGT 
GGTGTGGCTG TCAATCTGAT TCCAGGGTTA CCAGCATATA TCTTGTTTAT GTGTGTACGA 
CATGCTGACT ATCTGAACGA TGATCAGAAA GTAAGATCAT TGCTGACATC AACAATTAAC 
AGCATCAAAA AAGTCTTGAA GAAAAGAGGT GACGATTTTG AAACTGTCTC CTTCTGGCTC 
TCTAACACAT GTCGATTTTT GCACTGTTTG AAGCAATATA GTGGAGAGGA GGGCTTTATG 
AAGCACAACA CGTCTCGCCA GAATGAGCAC TGCCTCACCA ATTTTGACCT TGCTGAATAT 
CGGCAAGTAC TGAGTGACTT GGCCATTCAG ATCTATCAGC AGCTTGTGAG GGTGTTAGAG 
AACATTCTTC AGCCAATGAT AGTCTCAGGT ATGCTAGAGC ATGAAACAAT TCAGGGCGTA 
TCTGGGGTGA AGCCCACAGG GCTCAGAAAA CGAACCTCCA GTATCGCAGA TGAGGGCACC 
TACACACTGG ACTCCATCCT TCGGCAGCTC AACTCCTTCC ACTCAGTCAT GTGTCAGCAT 
GGCATGGACC CGGAGCTAAT CAAGCAGGTA GTCAAACAGA TGTTCTACAT TGTGGGCGCC 
ATCACCCTAA ACAACCTCCT CCTGCGCAAG GACATGTGCT CCTGGAGCAA GGGCATGCAG 
ATCAGGTACA ATGTCAGTCA ACTGGAAGAA TGGCTACGTG ACAAGAATCT AATGAACAGT 
GGGGCCAAGG AGACTCTGGA ACCTCTTATC CAGGCCGCTC AGCTTTTGCA AGTGAAAAAG 
AAAACTGACG ATGACGCAGA AGCCATCTGT TCCATGTGCA ACGCCCTGAC CACGGCCCAG 
ATTGTCAAAG TGTTGAATCT GTACACACCG GTTAATGAGT TTGAAGAAAG GGTCTCAGTT 
TCATTTATCC GCACTATACA GATGCGGTTA CGAGACAGGA AAGACTCTCC ACAGCTGCTC 
ATGGATGCAA AACACATCTT TCCTGTCACT TTTCCCTTTA ACCCATCCTC CCTAGCCCTA 
GAAACCATCC AGATCCCAGC CAGCCTGGGC CTGGGCTTCA TCGCACGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_006510829.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510892.1 Mus musculus myosin VA (Myo5a), transcript variant X3, mRNA.

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CloneID	OMu42556
Clone ID Related Accession (Same CDS sequence)	XM_006510829.3 , XM_006510829.4
Accession Version	XM_006510829.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510829.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGA 
TCATCTACTA TGGATTACCA GGAGTTGAAT GAGGATGGAG AGCTCTGGAT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCTTAGAA TCCCAGCTAC AGTCACAGAA AAGAAGCCAT 
GAGAATGAGG CTGAGGCCCT CCGTGGGGAG ATCCAGAGCC TAAAGGAAGA AAACAACCGG 
CAACAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCATG AGATCACCCG GCTGACCAAT GAAAACCTGG ATCTGATGGA ACAACTTGAA 
AAGCAGGATA AAACTGTCCG GAAACTGAAG AAACAACTGA AAGTCTTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGGCA GATGGAGAAC ATATCTCCAG GACAGATCAT CGATGAGCCT 
ATCCGGCCAG TCAACATTCC CCGGAAAGAA AAGGATTTCC AAGGAATGCT GGAGTACAAA 
AGGGAGGATG AGCAGAAGCT AGTGAAGAAC CTGATTCTAG AACTAAAGCC ACGTGGTGTG 
GCTGTCAATC TGATTCCAGG GTTACCAGCA TATATCTTGT TTATGTGTGT ACGACATGCT 
GACTATCTGA ACGATGATCA GAAAGTAAGA TCATTGCTGA CATCAACAAT TAACAGCATC 
AAAAAAGTCT TGAAGAAAAG AGGTGACGAT TTTGAAACTG TCTCCTTCTG GCTCTCTAAC 
ACATGTCGAT TTTTGCACTG TTTGAAGCAA TATAGTGGAG AGGAGGGCTT TATGAAGCAC 
AACACGTCTC GCCAGAATGA GCACTGCCTC ACCAATTTTG ACCTTGCTGA ATATCGGCAA 
GTACTGAGTG ACTTGGCCAT TCAGATCTAT CAGCAGCTTG TGAGGGTGTT AGAGAACATT 
CTTCAGCCAA TGATAGTCTC AGGTATGCTA GAGCATGAAA CAATTCAGGG CGTATCTGGG 
GTGAAGCCCA CAGGGCTCAG AAAACGAACC TCCAGTATCG CAGATGAGGG CACCTACACA 
CTGGACTCCA TCCTTCGGCA GCTCAACTCC TTCCACTCAG TCATGTGTCA GCATGGCATG 
GACCCGGAGC TAATCAAGCA GGTAGTCAAA CAGATGTTCT ACATTGTGGG CGCCATCACC 
CTAAACAACC TCCTCCTGCG CAAGGACATG TGCTCCTGGA GCAAGGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTGGA AGAATGGCTA CGTGACAAGA ATCTAATGAA CAGTGGGGCC 
AAGGAGACTC TGGAACCTCT TATCCAGGCC GCTCAGCTTT TGCAAGTGAA AAAGAAAACT 
GACGATGACG CAGAAGCCAT CTGTTCCATG TGCAACGCCC TGACCACGGC CCAGATTGTC 
AAAGTGTTGA ATCTGTACAC ACCGGTTAAT GAGTTTGAAG AAAGGGTCTC AGTTTCATTT 
ATCCGCACTA TACAGATGCG GTTACGAGAC AGGAAAGACT CTCCACAGCT GCTCATGGAT 
GCAAAACACA TCTTTCCTGT CACTTTTCCC TTTAACCCAT CCTCCCTAGC CCTAGAAACC 
ATCCAGATCC CAGCCAGCCT GGGCCTGGGC TTCATCGCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510892.1
CDS	619..6186
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSTMDYQELNEDGELWMVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510829.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510829.3

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGA 
TCATCTACTA TGGATTACCA GGAGTTGAAT GAGGATGGAG AGCTCTGGAT GGTTTATGAA 
GGGTTAAAAC AAGCCAACAG GCTCTTAGAA TCCCAGCTAC AGTCACAGAA AAGAAGCCAT 
GAGAATGAGG CTGAGGCCCT CCGTGGGGAG ATCCAGAGCC TAAAGGAAGA AAACAACCGG 
CAACAGCAGC TGCTGGCCCA GAACCTGCAG CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC 
CTGCAGCATG AGATCACCCG GCTGACCAAT GAAAACCTGG ATCTGATGGA ACAACTTGAA 
AAGCAGGATA AAACTGTCCG GAAACTGAAG AAACAACTGA AAGTCTTTGC CAAAAAAATT 
GGTGAACTAG AAGTGGGGCA GATGGAGAAC ATATCTCCAG GACAGATCAT CGATGAGCCT 
ATCCGGCCAG TCAACATTCC CCGGAAAGAA AAGGATTTCC AAGGAATGCT GGAGTACAAA 
AGGGAGGATG AGCAGAAGCT AGTGAAGAAC CTGATTCTAG AACTAAAGCC ACGTGGTGTG 
GCTGTCAATC TGATTCCAGG GTTACCAGCA TATATCTTGT TTATGTGTGT ACGACATGCT 
GACTATCTGA ACGATGATCA GAAAGTAAGA TCATTGCTGA CATCAACAAT TAACAGCATC 
AAAAAAGTCT TGAAGAAAAG AGGTGACGAT TTTGAAACTG TCTCCTTCTG GCTCTCTAAC 
ACATGTCGAT TTTTGCACTG TTTGAAGCAA TATAGTGGAG AGGAGGGCTT TATGAAGCAC 
AACACGTCTC GCCAGAATGA GCACTGCCTC ACCAATTTTG ACCTTGCTGA ATATCGGCAA 
GTACTGAGTG ACTTGGCCAT TCAGATCTAT CAGCAGCTTG TGAGGGTGTT AGAGAACATT 
CTTCAGCCAA TGATAGTCTC AGGTATGCTA GAGCATGAAA CAATTCAGGG CGTATCTGGG 
GTGAAGCCCA CAGGGCTCAG AAAACGAACC TCCAGTATCG CAGATGAGGG CACCTACACA 
CTGGACTCCA TCCTTCGGCA GCTCAACTCC TTCCACTCAG TCATGTGTCA GCATGGCATG 
GACCCGGAGC TAATCAAGCA GGTAGTCAAA CAGATGTTCT ACATTGTGGG CGCCATCACC 
CTAAACAACC TCCTCCTGCG CAAGGACATG TGCTCCTGGA GCAAGGGCAT GCAGATCAGG 
TACAATGTCA GTCAACTGGA AGAATGGCTA CGTGACAAGA ATCTAATGAA CAGTGGGGCC 
AAGGAGACTC TGGAACCTCT TATCCAGGCC GCTCAGCTTT TGCAAGTGAA AAAGAAAACT 
GACGATGACG CAGAAGCCAT CTGTTCCATG TGCAACGCCC TGACCACGGC CCAGATTGTC 
AAAGTGTTGA ATCTGTACAC ACCGGTTAAT GAGTTTGAAG AAAGGGTCTC AGTTTCATTT 
ATCCGCACTA TACAGATGCG GTTACGAGAC AGGAAAGACT CTCCACAGCT GCTCATGGAT 
GCAAAACACA TCTTTCCTGT CACTTTTCCC TTTAACCCAT CCTCCCTAGC CCTAGAAACC 
ATCCAGATCC CAGCCAGCCT GGGCCTGGGC TTCATCGCAC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_006510830.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510893.1 Mus musculus myosin VA (Myo5a), transcript variant X4, mRNA.

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CloneID	OMu42557
Clone ID Related Accession (Same CDS sequence)	XM_006510830.4 , XM_006510830.3
Accession Version	XM_006510830.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510830.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG ATCATCTACT 
ATGGATTACC AGGAGTTGAA TGAGGATGGA GAGCTCTGGA TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCTTAGA ATCCCAGCTA CAGTCACAGA AAAGAAGCCA TGAGAATGAG 
GCTGAGGCCC TCCGTGGGGA GATCCAGAGC CTAAAGGAAG AAAACAACCG GCAACAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCT GAGGCCCGCA TTGAGGCCAG CCTGCAGCAT 
GAGATCACCC GGCTGACCAA TGAAAACCTG GATCTGATGG AACAACTTGA AAAGCAGGAT 
AAAACTGTCC GGAAACTGAA GAAACAACTG AAAGTCTTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGGC AGATGGAGAA CATATCTCCA GGACAGATCA TCGATGAGCC TATCCGGCCA 
GTCAACATTC CCCGGAAAGA AAAGGATTTC CAAGGAATGC TGGAGTACAA AAGGGAGGAT 
GAGCAGAAGC TAGTGAAGAA CCTGATTCTA GAACTAAAGC CACGTGGTGT GGCTGTCAAT 
CTGATTCCAG GGTTACCAGC ATATATCTTG TTTATGTGTG TACGACATGC TGACTATCTG 
AACGATGATC AGAAAGTAAG ATCATTGCTG ACATCAACAA TTAACAGCAT CAAAAAAGTC 
TTGAAGAAAA GAGGTGACGA TTTTGAAACT GTCTCCTTCT GGCTCTCTAA CACATGTCGA 
TTTTTGCACT GTTTGAAGCA ATATAGTGGA GAGGAGGGCT TTATGAAGCA CAACACGTCT 
CGCCAGAATG AGCACTGCCT CACCAATTTT GACCTTGCTG AATATCGGCA AGTACTGAGT 
GACTTGGCCA TTCAGATCTA TCAGCAGCTT GTGAGGGTGT TAGAGAACAT TCTTCAGCCA 
ATGATAGTCT CAGGTATGCT AGAGCATGAA ACAATTCAGG GCGTATCTGG GGTGAAGCCC 
ACAGGGCTCA GAAAACGAAC CTCCAGTATC GCAGATGAGG GCACCTACAC ACTGGACTCC 
ATCCTTCGGC AGCTCAACTC CTTCCACTCA GTCATGTGTC AGCATGGCAT GGACCCGGAG 
CTAATCAAGC AGGTAGTCAA ACAGATGTTC TACATTGTGG GCGCCATCAC CCTAAACAAC 
CTCCTCCTGC GCAAGGACAT GTGCTCCTGG AGCAAGGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTGG AAGAATGGCT ACGTGACAAG AATCTAATGA ACAGTGGGGC CAAGGAGACT 
CTGGAACCTC TTATCCAGGC CGCTCAGCTT TTGCAAGTGA AAAAGAAAAC TGACGATGAC 
GCAGAAGCCA TCTGTTCCAT GTGCAACGCC CTGACCACGG CCCAGATTGT CAAAGTGTTG 
AATCTGTACA CACCGGTTAA TGAGTTTGAA GAAAGGGTCT CAGTTTCATT TATCCGCACT 
ATACAGATGC GGTTACGAGA CAGGAAAGAC TCTCCACAGC TGCTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACTTTTCC CTTTAACCCA TCCTCCCTAG CCCTAGAAAC CATCCAGATC 
CCAGCCAGCC TGGGCCTGGG CTTCATCGCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510893.1
CDS	619..6177
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSTMDYQELNEDGELWMVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510830.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510830.3

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG ATCATCTACT 
ATGGATTACC AGGAGTTGAA TGAGGATGGA GAGCTCTGGA TGGTTTATGA AGGGTTAAAA 
CAAGCCAACA GGCTCTTAGA ATCCCAGCTA CAGTCACAGA AAAGAAGCCA TGAGAATGAG 
GCTGAGGCCC TCCGTGGGGA GATCCAGAGC CTAAAGGAAG AAAACAACCG GCAACAGCAG 
CTGCTGGCCC AGAACCTGCA GCTGCCCCCT GAGGCCCGCA TTGAGGCCAG CCTGCAGCAT 
GAGATCACCC GGCTGACCAA TGAAAACCTG GATCTGATGG AACAACTTGA AAAGCAGGAT 
AAAACTGTCC GGAAACTGAA GAAACAACTG AAAGTCTTTG CCAAAAAAAT TGGTGAACTA 
GAAGTGGGGC AGATGGAGAA CATATCTCCA GGACAGATCA TCGATGAGCC TATCCGGCCA 
GTCAACATTC CCCGGAAAGA AAAGGATTTC CAAGGAATGC TGGAGTACAA AAGGGAGGAT 
GAGCAGAAGC TAGTGAAGAA CCTGATTCTA GAACTAAAGC CACGTGGTGT GGCTGTCAAT 
CTGATTCCAG GGTTACCAGC ATATATCTTG TTTATGTGTG TACGACATGC TGACTATCTG 
AACGATGATC AGAAAGTAAG ATCATTGCTG ACATCAACAA TTAACAGCAT CAAAAAAGTC 
TTGAAGAAAA GAGGTGACGA TTTTGAAACT GTCTCCTTCT GGCTCTCTAA CACATGTCGA 
TTTTTGCACT GTTTGAAGCA ATATAGTGGA GAGGAGGGCT TTATGAAGCA CAACACGTCT 
CGCCAGAATG AGCACTGCCT CACCAATTTT GACCTTGCTG AATATCGGCA AGTACTGAGT 
GACTTGGCCA TTCAGATCTA TCAGCAGCTT GTGAGGGTGT TAGAGAACAT TCTTCAGCCA 
ATGATAGTCT CAGGTATGCT AGAGCATGAA ACAATTCAGG GCGTATCTGG GGTGAAGCCC 
ACAGGGCTCA GAAAACGAAC CTCCAGTATC GCAGATGAGG GCACCTACAC ACTGGACTCC 
ATCCTTCGGC AGCTCAACTC CTTCCACTCA GTCATGTGTC AGCATGGCAT GGACCCGGAG 
CTAATCAAGC AGGTAGTCAA ACAGATGTTC TACATTGTGG GCGCCATCAC CCTAAACAAC 
CTCCTCCTGC GCAAGGACAT GTGCTCCTGG AGCAAGGGCA TGCAGATCAG GTACAATGTC 
AGTCAACTGG AAGAATGGCT ACGTGACAAG AATCTAATGA ACAGTGGGGC CAAGGAGACT 
CTGGAACCTC TTATCCAGGC CGCTCAGCTT TTGCAAGTGA AAAAGAAAAC TGACGATGAC 
GCAGAAGCCA TCTGTTCCAT GTGCAACGCC CTGACCACGG CCCAGATTGT CAAAGTGTTG 
AATCTGTACA CACCGGTTAA TGAGTTTGAA GAAAGGGTCT CAGTTTCATT TATCCGCACT 
ATACAGATGC GGTTACGAGA CAGGAAAGAC TCTCCACAGC TGCTCATGGA TGCAAAACAC 
ATCTTTCCTG TCACTTTTCC CTTTAACCCA TCCTCCCTAG CCCTAGAAAC CATCCAGATC 
CCAGCCAGCC TGGGCCTGGG CTTCATCGCA CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_006510831.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510894.1 Mus musculus myosin VA (Myo5a), transcript variant X5, mRNA.

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CloneID	OMu42558
Clone ID Related Accession (Same CDS sequence)	XM_006510831.3 , XM_006510831.4
Accession Version	XM_006510831.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG GCTCTTAGAA 
TCCCAGCTAC AGTCACAGAA AAGAAGCCAT GAGAATGAGG CTGAGGCCCT CCGTGGGGAG 
ATCCAGAGCC TAAAGGAAGA AAACAACCGG CAACAGCAGC TGCTGGCCCA GAACCTGCAG 
CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC CTGCAGCATG AGATCACCCG GCTGACCAAT 
GAAAACCTGT ATTTTGAGGA ATTATATGCA GATGACCCTA AGAAGTATCA ATCCTATCGG 
ATTTCACTTT ACAAAAGGAT GATTGATCTG ATGGAACAAC TTGAAAAGCA GGATAAAACT 
GTCCGGAAAC TGAAGAAACA ACTGAAAGTC TTTGCCAAAA AAATTGGTGA ACTAGAAGTG 
GGGCAGATGG AGAACATATC TCCAGGACAG ATCATCGATG AGCCTATCCG GCCAGTCAAC 
ATTCCCCGGA AAGAAAAGGA TTTCCAAGGA ATGCTGGAGT ACAAAAGGGA GGATGAGCAG 
AAGCTAGTGA AGAACCTGAT TCTAGAACTA AAGCCACGTG GTGTGGCTGT CAATCTGATT 
CCAGGGTTAC CAGCATATAT CTTGTTTATG TGTGTACGAC ATGCTGACTA TCTGAACGAT 
GATCAGAAAG TAAGATCATT GCTGACATCA ACAATTAACA GCATCAAAAA AGTCTTGAAG 
AAAAGAGGTG ACGATTTTGA AACTGTCTCC TTCTGGCTCT CTAACACATG TCGATTTTTG 
CACTGTTTGA AGCAATATAG TGGAGAGGAG GGCTTTATGA AGCACAACAC GTCTCGCCAG 
AATGAGCACT GCCTCACCAA TTTTGACCTT GCTGAATATC GGCAAGTACT GAGTGACTTG 
GCCATTCAGA TCTATCAGCA GCTTGTGAGG GTGTTAGAGA ACATTCTTCA GCCAATGATA 
GTCTCAGGTA TGCTAGAGCA TGAAACAATT CAGGGCGTAT CTGGGGTGAA GCCCACAGGG 
CTCAGAAAAC GAACCTCCAG TATCGCAGAT GAGGGCACCT ACACACTGGA CTCCATCCTT 
CGGCAGCTCA ACTCCTTCCA CTCAGTCATG TGTCAGCATG GCATGGACCC GGAGCTAATC 
AAGCAGGTAG TCAAACAGAT GTTCTACATT GTGGGCGCCA TCACCCTAAA CAACCTCCTC 
CTGCGCAAGG ACATGTGCTC CTGGAGCAAG GGCATGCAGA TCAGGTACAA TGTCAGTCAA 
CTGGAAGAAT GGCTACGTGA CAAGAATCTA ATGAACAGTG GGGCCAAGGA GACTCTGGAA 
CCTCTTATCC AGGCCGCTCA GCTTTTGCAA GTGAAAAAGA AAACTGACGA TGACGCAGAA 
GCCATCTGTT CCATGTGCAA CGCCCTGACC ACGGCCCAGA TTGTCAAAGT GTTGAATCTG 
TACACACCGG TTAATGAGTT TGAAGAAAGG GTCTCAGTTT CATTTATCCG CACTATACAG 
ATGCGGTTAC GAGACAGGAA AGACTCTCCA CAGCTGCTCA TGGATGCAAA ACACATCTTT 
CCTGTCACTT TTCCCTTTAA CCCATCCTCC CTAGCCCTAG AAACCATCCA GATCCCAGCC 
AGCCTGGGCC TGGGCTTCAT CGCACGGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510894.1
CDS	619..6171
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510831.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510831.3

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGA ATACAATGAC AGATTCCACA ATTCTTTTAG AAGATGTACA GAAAATGAAA 
GACAAAGGTG AAATAGCACA AGCATATATT GGTTTGAAAG AAACAAACAG GCTCTTAGAA 
TCCCAGCTAC AGTCACAGAA AAGAAGCCAT GAGAATGAGG CTGAGGCCCT CCGTGGGGAG 
ATCCAGAGCC TAAAGGAAGA AAACAACCGG CAACAGCAGC TGCTGGCCCA GAACCTGCAG 
CTGCCCCCTG AGGCCCGCAT TGAGGCCAGC CTGCAGCATG AGATCACCCG GCTGACCAAT 
GAAAACCTGT ATTTTGAGGA ATTATATGCA GATGACCCTA AGAAGTATCA ATCCTATCGG 
ATTTCACTTT ACAAAAGGAT GATTGATCTG ATGGAACAAC TTGAAAAGCA GGATAAAACT 
GTCCGGAAAC TGAAGAAACA ACTGAAAGTC TTTGCCAAAA AAATTGGTGA ACTAGAAGTG 
GGGCAGATGG AGAACATATC TCCAGGACAG ATCATCGATG AGCCTATCCG GCCAGTCAAC 
ATTCCCCGGA AAGAAAAGGA TTTCCAAGGA ATGCTGGAGT ACAAAAGGGA GGATGAGCAG 
AAGCTAGTGA AGAACCTGAT TCTAGAACTA AAGCCACGTG GTGTGGCTGT CAATCTGATT 
CCAGGGTTAC CAGCATATAT CTTGTTTATG TGTGTACGAC ATGCTGACTA TCTGAACGAT 
GATCAGAAAG TAAGATCATT GCTGACATCA ACAATTAACA GCATCAAAAA AGTCTTGAAG 
AAAAGAGGTG ACGATTTTGA AACTGTCTCC TTCTGGCTCT CTAACACATG TCGATTTTTG 
CACTGTTTGA AGCAATATAG TGGAGAGGAG GGCTTTATGA AGCACAACAC GTCTCGCCAG 
AATGAGCACT GCCTCACCAA TTTTGACCTT GCTGAATATC GGCAAGTACT GAGTGACTTG 
GCCATTCAGA TCTATCAGCA GCTTGTGAGG GTGTTAGAGA ACATTCTTCA GCCAATGATA 
GTCTCAGGTA TGCTAGAGCA TGAAACAATT CAGGGCGTAT CTGGGGTGAA GCCCACAGGG 
CTCAGAAAAC GAACCTCCAG TATCGCAGAT GAGGGCACCT ACACACTGGA CTCCATCCTT 
CGGCAGCTCA ACTCCTTCCA CTCAGTCATG TGTCAGCATG GCATGGACCC GGAGCTAATC 
AAGCAGGTAG TCAAACAGAT GTTCTACATT GTGGGCGCCA TCACCCTAAA CAACCTCCTC 
CTGCGCAAGG ACATGTGCTC CTGGAGCAAG GGCATGCAGA TCAGGTACAA TGTCAGTCAA 
CTGGAAGAAT GGCTACGTGA CAAGAATCTA ATGAACAGTG GGGCCAAGGA GACTCTGGAA 
CCTCTTATCC AGGCCGCTCA GCTTTTGCAA GTGAAAAAGA AAACTGACGA TGACGCAGAA 
GCCATCTGTT CCATGTGCAA CGCCCTGACC ACGGCCCAGA TTGTCAAAGT GTTGAATCTG 
TACACACCGG TTAATGAGTT TGAAGAAAGG GTCTCAGTTT CATTTATCCG CACTATACAG 
ATGCGGTTAC GAGACAGGAA AGACTCTCCA CAGCTGCTCA TGGATGCAAA ACACATCTTT 
CCTGTCACTT TTCCCTTTAA CCCATCCTCC CTAGCCCTAG AAACCATCCA GATCCCAGCC 
AGCCTGGGCC TGGGCTTCAT CGCACGGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo5a ( XM_006510832.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510895.1 Mus musculus myosin VA (Myo5a), transcript variant X6, mRNA.

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CloneID	OMu42559
Clone ID Related Accession (Same CDS sequence)	XM_006510832.4 , XM_006510832.3
Accession Version	XM_006510832.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Va isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGG 
CTCTTAGAAT CCCAGCTACA GTCACAGAAA AGAAGCCATG AGAATGAGGC TGAGGCCCTC 
CGTGGGGAGA TCCAGAGCCT AAAGGAAGAA AACAACCGGC AACAGCAGCT GCTGGCCCAG 
AACCTGCAGC TGCCCCCTGA GGCCCGCATT GAGGCCAGCC TGCAGCATGA GATCACCCGG 
CTGACCAATG AAAACCTGGA TCTGATGGAA CAACTTGAAA AGCAGGATAA AACTGTCCGG 
AAACTGAAGA AACAACTGAA AGTCTTTGCC AAAAAAATTG GTGAACTAGA AGTGGGGCAG 
ATGGAGAACA TATCTCCAGG ACAGATCATC GATGAGCCTA TCCGGCCAGT CAACATTCCC 
CGGAAAGAAA AGGATTTCCA AGGAATGCTG GAGTACAAAA GGGAGGATGA GCAGAAGCTA 
GTGAAGAACC TGATTCTAGA ACTAAAGCCA CGTGGTGTGG CTGTCAATCT GATTCCAGGG 
TTACCAGCAT ATATCTTGTT TATGTGTGTA CGACATGCTG ACTATCTGAA CGATGATCAG 
AAAGTAAGAT CATTGCTGAC ATCAACAATT AACAGCATCA AAAAAGTCTT GAAGAAAAGA 
GGTGACGATT TTGAAACTGT CTCCTTCTGG CTCTCTAACA CATGTCGATT TTTGCACTGT 
TTGAAGCAAT ATAGTGGAGA GGAGGGCTTT ATGAAGCACA ACACGTCTCG CCAGAATGAG 
CACTGCCTCA CCAATTTTGA CCTTGCTGAA TATCGGCAAG TACTGAGTGA CTTGGCCATT 
CAGATCTATC AGCAGCTTGT GAGGGTGTTA GAGAACATTC TTCAGCCAAT GATAGTCTCA 
GGTATGCTAG AGCATGAAAC AATTCAGGGC GTATCTGGGG TGAAGCCCAC AGGGCTCAGA 
AAACGAACCT CCAGTATCGC AGATGAGGGC ACCTACACAC TGGACTCCAT CCTTCGGCAG 
CTCAACTCCT TCCACTCAGT CATGTGTCAG CATGGCATGG ACCCGGAGCT AATCAAGCAG 
GTAGTCAAAC AGATGTTCTA CATTGTGGGC GCCATCACCC TAAACAACCT CCTCCTGCGC 
AAGGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ACAATGTCAG TCAACTGGAA 
GAATGGCTAC GTGACAAGAA TCTAATGAAC AGTGGGGCCA AGGAGACTCT GGAACCTCTT 
ATCCAGGCCG CTCAGCTTTT GCAAGTGAAA AAGAAAACTG ACGATGACGC AGAAGCCATC 
TGTTCCATGT GCAACGCCCT GACCACGGCC CAGATTGTCA AAGTGTTGAA TCTGTACACA 
CCGGTTAATG AGTTTGAAGA AAGGGTCTCA GTTTCATTTA TCCGCACTAT ACAGATGCGG 
TTACGAGACA GGAAAGACTC TCCACAGCTG CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACTTTTCCCT TTAACCCATC CTCCCTAGCC CTAGAAACCA TCCAGATCCC AGCCAGCCTG 
GGCCTGGGCT TCATCGCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510895.1
CDS	619..6105
Translation	MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTIPPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAATIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV

Target ORF information:

RefSeq Version	XM_006510832.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VA (Myo5a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510832.3

ORF Insert Sequence:

ATGGCCGCGT CCGAGCTCTA CACCAAGTTT GCCAGGGTTT GGATCCCTGA TCCTGAGGAA 
GTGTGGAAAT CGGCAGAGTT GCTCAAGGAT TATAAGCCTG GAGATAAAGT GCTCCTGCTT 
CACCTCGAGG AAGGGAAGGA TTTGGAATAC CGTCTAGACC CAAAGACCGG TGAGCTCCCT 
CACTTACGGA ACCCTGACAT ACTTGTTGGA GAAAATGACC TCACAGCCCT CAGCTACCTT 
CACGAGCCCG CTGTGCTACA TAATCTCCGA GTTCGCTTCA TCGATTCCAA ACTTATTTAT 
ACGTATTGTG GAATAGTTCT GGTAGCTATA AATCCCTATG AGCAGCTGCC TATTTATGGA 
GAAGATATTA TTAATGCCTA CAGTGGCCAG AACATGGGTG ACATGGATCC TCACATCTTC 
GCAGTAGCTG AAGAGGCTTA CAAGCAAATG GCAAGGGATG AACGAAATCA GTCCATCATT 
GTAAGTGGAG AGTCAGGCGC AGGGAAGACG GTCTCTGCTA AGTATGCCAT GCGGTACTTC 
GCAACTGTAA GTGGCTCTGC CAGTGAGGCC AATGTTGAGG AAAAGGTCTT GGCCTCCAAC 
CCCATCATGG AGTCAATTGG AAATGCCAAA ACAACCAGGA ATGATAATAG CAGCCGGTTT 
GGAAAATATA TTGAAATTGG TTTTGACAAG AGGTACAGAA TCATCGGTGC CAATATGAGA 
ACTTACCTTT TAGAGAAATC CAGAGTGGTG TTCCAGGCAG AAGAGGAGAG AAACTACCAT 
ATCTTCTATC AGCTCTGTGC TTCGGCAAAG TTACCTGAGT TTAAAATGCT GCGGTTAGGA 
AATGCAGATA GTTTTCATTA CACGAAGCAA GGAGGCAGCC CTATGATAGA AGGAGTAGAT 
GATGCGAAGG AGATGGCGCA CACCAGGCAG GCCTGCACTC TGCTGGGAAT TAGTGAATCT 
TACCAAATGG GAATTTTTCG AATACTTGCT GGCATTCTTC ACTTAGGCAA TGTTGGATTT 
GCATCTCGGG ATTCAGACAG CTGCACAATA CCTCCCAAGC ACGAACCTCT TACCATCTTC 
TGTGACCTTA TGGGTGTGGA TTATGAAGAG ATGTGTCACT GGCTCTGCCA CCGAAAGCTG 
GCTACTGCCA CAGAGACATA CATCAAGCCC ATCTCCAAGC TGCAGGCCAC AAATGCCCGA 
GATGCTTTAG CAAAGCACAT CTATGCAAAG CTCTTTAACT GGATTGTTGA CCACGTCAAT 
CAGGCTCTCC ATTCTGCTGT CAAGCAGCAC TCTTTCATCG GCGTGCTGGA CATCTATGGA 
TTTGAAACAT TTGAAATAAA TAGTTTTGAA CAGTTCTGCA TAAATTATGC AAATGAAAAA 
CTACAACAAC AATTCAACAT GCATGTCTTC AAATTGGAAC AGGAGGAATA TATGAAGGAA 
CAGATTCCAT GGACACTTAT AGATTTCTAT GATAATCAGC CTTGTATCAA TCTTATAGAA 
TCTAAACTGG GAATTCTCGA TTTGCTGGAT GAGGAATGTA AGATGCCTAA AGGCACAGAT 
GACACATGGG CCCAAAAACT GTACAACACA CATTTGAACA AATGTGCCCT CTTTGAGAAG 
CCCCGCATGT CAAACAAAGC TTTCATCATC AAACATTTTG CTGACAAAGT GGAGTACCAG 
TGTGAAGGTT TTCTTGAAAA GAATAAAGAT ACTGTTTTTG AAGAACAAAT TAAAGTCCTT 
AAGTCAAGCA AGTTTAAGAT GCTACCAGAG CTATTTCAAG ATGATGAGAA GGCCATCAGT 
CCCACCTCTG CCACCTCCTC AGGGCGCACT CCTCTCACTC GAGTTCCTGT AAAGCCCACC 
AAGGGCCGAC CTGGCCAGAC TGCCAAAGAG CACAAGAAGA CAGTGGGACA TCAGTTTCGA 
AACTCCCTTC ACCTGCTTAT GGAAACCCTT AATGCCACTA CTCCTCACTA TGTACGCTGT 
ATTAAGCCTA ATGATTTCAA GTTTCCATTC ACATTTGATG AGAAGAGGGC AGTGCAGCAG 
CTAAGAGCAT GTGGTGTCCT GGAGACCATC CGGATCAGCG CAGCAGGGTT TCCCTCACGG 
TGGACTTACC AAGAGTTTTT CAGCCGGTAC CGGGTCCTAA TGAAGCAAAA AGATGTGCTG 
GGAGATAGAA AGCAAACGTG CAAGAATGTA TTAGAGAAAC TAATATTGGA CAAGGATAAA 
TACCAGTTTG GTAAGACAAA GATCTTTTTC CGTGCTGGTC AAGTGGCCTA TCTTGAAAAA 
TTGAGGGCTG ACAAACTTCG GGCTGCCTGC ATCCGGATCC AGAAGACCAT TCGTGGGTGG 
CTTCTAAGGA AGAGATACCT GTGTATGCAG AGGGCAGCCA TCACAGTGCA GCGATACGTG 
CGGGGCTATC AGGCTCGATG CTATGCTAAG TTTCTGCGCA GAACCAAGGC AGCAACCACC 
ATTCAAAAGT ACTGGCGCAT GTATGTGGTC CGCAGGAGGT ACAAGATTAG ACGAGCTGCC 
ACGATTGTTA TTCAGTCTTA CTTGAGAGGC TACTTGACAA GAAATAGGTA TCGCAAGATA 
CTCCGTGAAT ACAAAGCAGT CATCATTCAG AAACGTGTCC GTGGCTGGCT GGCCCGTACA 
CATTATAAGA GGACCATGAA AGCCATCGTC TACCTTCAGT GCTGCTTCCG GCGGATGATG 
GCCAAGCGTG AGCTGAAGAA ACTCAAAATT GAGGCTCGCT CTGTGGAACG CTACAAGAAG 
CTCCATATTG GCATGGAGAA CAAGATTATG CAGCTGCAGC GCAAAGTGGA TGAGCAGAAT 
AAAGACTACA AATGCCTCAT GGAGAAACTG ACCAATCTGG AAGGAGTATA CAACTCTGAG 
ACTGAAAAAC TACGAAATGA TGTAGAACGT CTTCAGCTAA GTGAAGAGGA AGCTAAGGTT 
GCCACTGGGC GGGTCCTTAG TCTGCAGGAA GAAATTGCAA AACTCCGAAA AGACCTGGAA 
CAAACTCGAT CAGAGAAAAA GTCTATTGAA GAAAGAGCAG ATAAATACAA ACAAGAAACT 
GACCAGCTGG TGTCAAACTT GAAGGAAGAA AATACTTTGC TGAAGCAGGA AAAGGAGACC 
CTCAACCACC GCATTGTGGA GCAGGCGAAG GAGATGACAG AAACTATGGA GAGGAAGTTA 
GTAGAAGAGA CAAAACAACT GGAGCTTGAC CTGAATGATG AGAGGCTGAG GTATCAGAAC 
CTTCTGAATG AGTTCAGTCG CCTGGAGGAG CGCTATGATG ACCTCAAGGA AGAGATGACC 
CTGATGCTGA ATGTGCCTAA GCCAGGACAC AAGAGAACAG ACTCTACCCA CAGCAGTAAT 
GAGTCTGAAT ACACCTTCAG CTCTGAGTTT GCAGAAACTG AAGACATTGC ACCAAGAACA 
GAGGAGCCAA TTGAGAAGAA GGTGCCTCTG GATATGTCAC TATTCCTTAA GCTCCAGAAG 
CGTGTCACAG AGCTGGAACA GGAGAAGCAG CTGATGCAGG ATGAGCTGGA CCGCAAGGAG 
GAGCAGGTGT TCCGCAGCAA GGCAAAGGAA GAAGAAAGGC CACAGATAAG AGGAGCTGAA 
CTAGAGTATG AGTCTCTCAA GCGTCAAGAA CTGGAGTCAG AAAACAAAAA ACTGAAAAAC 
GAGCTGAATG AGTTGCGCAA AGCCCTCAGT GAGAAAAGTG CCCCAGAAGT GACTGCGCCA 
GGTGCGCCTG CTTACCGAGT CCTCATGGAA CAGCTGACCT CCGTGAGCGA GGAGCTCGAC 
GTGCGCAAGG AGGAAGTCCT CATCTTGAGG TCGCAGCTGG TGAGCCAAAA AGAAGCCATC 
CAACCCAAGG ATGACAAGAA TACAATGACA GATTCCACAA TTCTTTTAGA AGATGTACAG 
AAAATGAAAG ACAAAGGTGA AATAGCACAA GCATATATTG GTTTGAAAGA AACAAACAGG 
CTCTTAGAAT CCCAGCTACA GTCACAGAAA AGAAGCCATG AGAATGAGGC TGAGGCCCTC 
CGTGGGGAGA TCCAGAGCCT AAAGGAAGAA AACAACCGGC AACAGCAGCT GCTGGCCCAG 
AACCTGCAGC TGCCCCCTGA GGCCCGCATT GAGGCCAGCC TGCAGCATGA GATCACCCGG 
CTGACCAATG AAAACCTGGA TCTGATGGAA CAACTTGAAA AGCAGGATAA AACTGTCCGG 
AAACTGAAGA AACAACTGAA AGTCTTTGCC AAAAAAATTG GTGAACTAGA AGTGGGGCAG 
ATGGAGAACA TATCTCCAGG ACAGATCATC GATGAGCCTA TCCGGCCAGT CAACATTCCC 
CGGAAAGAAA AGGATTTCCA AGGAATGCTG GAGTACAAAA GGGAGGATGA GCAGAAGCTA 
GTGAAGAACC TGATTCTAGA ACTAAAGCCA CGTGGTGTGG CTGTCAATCT GATTCCAGGG 
TTACCAGCAT ATATCTTGTT TATGTGTGTA CGACATGCTG ACTATCTGAA CGATGATCAG 
AAAGTAAGAT CATTGCTGAC ATCAACAATT AACAGCATCA AAAAAGTCTT GAAGAAAAGA 
GGTGACGATT TTGAAACTGT CTCCTTCTGG CTCTCTAACA CATGTCGATT TTTGCACTGT 
TTGAAGCAAT ATAGTGGAGA GGAGGGCTTT ATGAAGCACA ACACGTCTCG CCAGAATGAG 
CACTGCCTCA CCAATTTTGA CCTTGCTGAA TATCGGCAAG TACTGAGTGA CTTGGCCATT 
CAGATCTATC AGCAGCTTGT GAGGGTGTTA GAGAACATTC TTCAGCCAAT GATAGTCTCA 
GGTATGCTAG AGCATGAAAC AATTCAGGGC GTATCTGGGG TGAAGCCCAC AGGGCTCAGA 
AAACGAACCT CCAGTATCGC AGATGAGGGC ACCTACACAC TGGACTCCAT CCTTCGGCAG 
CTCAACTCCT TCCACTCAGT CATGTGTCAG CATGGCATGG ACCCGGAGCT AATCAAGCAG 
GTAGTCAAAC AGATGTTCTA CATTGTGGGC GCCATCACCC TAAACAACCT CCTCCTGCGC 
AAGGACATGT GCTCCTGGAG CAAGGGCATG CAGATCAGGT ACAATGTCAG TCAACTGGAA 
GAATGGCTAC GTGACAAGAA TCTAATGAAC AGTGGGGCCA AGGAGACTCT GGAACCTCTT 
ATCCAGGCCG CTCAGCTTTT GCAAGTGAAA AAGAAAACTG ACGATGACGC AGAAGCCATC 
TGTTCCATGT GCAACGCCCT GACCACGGCC CAGATTGTCA AAGTGTTGAA TCTGTACACA 
CCGGTTAATG AGTTTGAAGA AAGGGTCTCA GTTTCATTTA TCCGCACTAT ACAGATGCGG 
TTACGAGACA GGAAAGACTC TCCACAGCTG CTCATGGATG CAAAACACAT CTTTCCTGTC 
ACTTTTCCCT TTAACCCATC CTCCCTAGCC CTAGAAACCA TCCAGATCCC AGCCAGCCTG 
GGCCTGGGCT TCATCGCACG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Myo5a
Entrez Gene ID	17918
Full Name	myosin VA
Synonyms	9630007J19Rik,AI413174,AI661011,Dbv,MVa,Myo5,MyoVA,Sev-1,d,d-120J,flail,flr
General protein information	Preferred Names myosin VA Names unconventional myosin-Va dilute lethal-20J protein dilute myosin heavy chain, non-muscle flailer myosin-Va
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 D\|9 42.26 cM

	MYO5A	XP_001084476.1
Canis lupus familiaris (dog)	MYO5A	XP_005638745.1
Mus musculus (house mouse)	Myo5a	NP_034994.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006479	XP_316519.4
Kluyveromyces lactis	KLLA0A03905g	XP_451171.1
Magnaporthe oryzae (rice blast fungus)	MGG_16213	XP_003709659.1
Arabidopsis thaliana (thale cress)	XIC	NP_172349.2
Oryza sativa (rice)	Os06g0488200	NP_001057670.1
Arabidopsis thaliana (thale cress)	XIE	NP_175858.1
Bos taurus (cattle)	MYO5A	XP_005211933.1
Rattus norvegicus (Norway rat)	Myo5a	NP_071514.1
Gallus gallus (chicken)	MYO5A	NP_990631.1
Neurospora crassa	NCU11354	XP_001728528.1
Xenopus tropicalis (tropical clawed frog)	myo5a	XP_004912752.1
Pan troglodytes (chimpanzee)	MYO5A	XP_510412.2
Danio rerio (zebrafish)	myo5aa	XP_005173731.1
Drosophila melanogaster (fruit fly)	didum	NP_724569.1
Caenorhabditis elegans (roundworm)	hum-2	NP_001023886.1
Saccharomyces cerevisiae (baker's yeast)	MYO2	NP_014971.1
Homo sapiens (human)	MYO5A	NP_000250.3
Eremothecium gossypii	AGOS_ADR354W	NP_984450.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Myo5a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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Target ORF information:

Target ORF information:

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ORF Insert Sequence:

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