ORF » Species Summary » Danio rerio » robo1 cDNA ORF clone

robo1 cDNA ORF clone, Danio rerio(zebrafish)

The following robo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa55064 XM_017359213.1
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa159830 NM_001365167.1
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa55059 NM_001365166.1
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa148914 XM_017359205.2
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa148915 XM_017359206.2
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa148916 XM_017359207.2
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa148917 XM_017359212.2
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa148918 XM_021481331.1
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa148919 XM_021481332.1
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55055 XM_017359204.1
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa55056 XM_017359205.1
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa55057 XM_017359206.1
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa55058 XM_017359207.1
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa55059 XM_017359208.2
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa55060 XM_017359209.2
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55061 XM_017359210.2
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55062 XM_017359211.2
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55063 XM_017359212.1
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa39335 NM_001309824.2
Latest version!		 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
ODa55060 XM_017359209.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa39335 NM_001309824.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa55062 XM_017359211.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55059 XM_017359208.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa55061 XM_017359210.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    robo1 ( XM_017359213.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214702.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID ODa55064
    Clone ID Related Accession (Same CDS sequence) XM_017359213.1
    Accession Version XM_017359213.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2583bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 1 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGTTCA GAGATTGCAA TTGCAGATTT TTTATACGGA ACTGTGTCCT GCTTATGGTT 
    TGGTATCAGG GGTATTGGGG CGCCACTATG TTGCAGATGG CCCGATACAC TTTACTGGAG 
    GACTCAAAGT CTGTATTACT TCCAACAGAC ACGGGGACTC AGGATGGGAC TAGCAATAGG 
    GCCACAGAGA CCCCCACTGC AGAACACGTT GACGACAACT CTCTGGGCTA CACAGGATCC 
    CGTCTGCGGC AGGAAGATTA CGCACCCCGT ATTGTTGAAC ACCCCTCTGA TCTGATCGTA 
    TCCAAAGGCG AACCGGCCAC CCTAAACTGC AAGGCTGAAG GACGTCCCAC GCCCACAGTG 
    GAGTGGTACA AAGATGGAGA ACGAGTTGAA ACCGACCGGG ACAATCCTCG ATCCCACCGC 
    ATGCTCCTGC CCACCGGATC TCTCTTCTTT CTCCGCATTG TTCACGGCCG GCGCAGCAAA 
    CCCGACGAAG GCTCCTACGT TTGTGTGGCA CGTAACTATC TGGGAGAAGC CGTCAGCCAC 
    AACGCCTCGC TGGAAGTAGC TATTCTCCGA GATGATTTCC GACAGAACCC TGTGGACGTG 
    ATGGTGGCAG CGGGGGAGCC AGCTGTAATG GAGTGCCAGC CGCCCCGAGG ACACCCAGAG 
    CCCACCATCT CCTGGAAGAA AGATGGGGTC AATATAGACG ACAGAGACGA GAGGATAACG 
    ATACGAGGAG GAAAGCTCAT GATCACCAAC ACCAGAAAAA CGGACGCGGG GAAGTACGTG 
    TGCGTGGGAA CCAACATGAT GGGGGAACGA GAGAGCGAGA TCGCGGAGTT GACTGTTCTC 
    GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT 
    GAGTTCAGAT GTGAAGCTCA AGGAGACCCC GTGCCCACCA TCCGATGGAG GAAAGAGGAC 
    GGAGAACTGC CCAAGGGAAG GTATGAGATC TGGGAGGACC ACACTCTCAA ACTGAAGAGG 
    CTGACTTCAG GAGACGCCGG TACTTACACC TGTCAGGCGG AGAACATGAT GGGCAAGACG 
    GAGGCCAGCG CCACCCTCAC CGTCCACGTT GTGTCTGTTC CTCCAGTGTT TGTGGTCCGA 
    CCCAGGAATC AGGTGGCGGG TATGGGCCGC ACCGTCACCT TTCAGTGTGA AGCCACAGGA 
    AGTCCTCAGC CTGCAATATT CTGGCAGAAA GAAGGCAGTC AGAATCTCCT GTTCTCGTCT 
    CAGCCTCCTC CTCCGTCCAG CAGATTCTCT GTGTCTCAGA CTGGAGATCT GACCATCACA 
    GCAGTTGAGC GTTCAGATGG AGGATATTAT AGCTGTCAGG CTCTCAACAT CGCTGGCAGT 
    GTGATCACCA AAGCCCTGCT GGAGGTTACA GACGTGATTT CAGACAGGCC TCCACCAATC 
    GTCCGTCAGG GTCCAACCAA CCAGACTGTG GCAGTGGACG GGACGGTGGT TCTGAGCTGC 
    CAAGCCACCG GGACACCGAT TCCTACCATC TTATGGAGGA AAGATGGAGT TCTGGTGTCC 
    ACACATGATT CCCGACTCAA GCAGCTGGAC ACTGGAGCCC TGCAGATTCG ATACGCAAAG 
    CTGGGCGACA CTGGCATGTA CACCTGCACG GCTTCCACCC CGAGCGGAGA GGCGTCCTGG 
    AAGGCCTACC TGGAGGTGCA AGAGTTTGGT GCTCCAGTTC AACCGGGCCG TCCCACAGAT 
    CCCAACCTCA TCCCCAGCGC CCCATCTAAA CCAGACGTCA CGGATGTCAG CCGCACCTCC 
    GTGTCTCTTT CCTGGAAGCC GAATCTGAAC GCTGGAGCCA CACCCACCTC ATACGTCATT 
    GAGGCCTTCA GCCATGCAGC AGGCAGCAGC TGGCAGACGC TCGCCGATCA TGTGAAGACG 
    GAAAGCTTCG TGCTTAAGGG TCTGAAGCCA AGTGCCGTCT ACCTGTTTCT GGTGAGAGCG 
    GCCAATGCGT ACGGCCTGAG CGACCCCAGT CCCATCAGCG ACGCTGTCAA AACACAAGAC 
    GTCCCACCAA CCAGCCAGGG AGTGGAGCAT GGCCAGATCC AGAGAGAACT GGGAGATGTC 
    GTCATTCACC TTCACAATCC CACAATTCTC TCATCGTCTT CCGTCAGAGT CCAGTGGACT 
    GTGGAGCAGC AGTCTCAGTA TATTCAGGGC TATAAGGTGA TGTACCGTCC GTCTCCAGAA 
    GGCGCTCCGC AGCGAGTGGA TTGGGCCATG TTTGAGGTCG GAGCCCCGGG GGAGCACAGC 
    GCTGTCGTCA CCCAGCTCAA GAAGGGCATC ACCTATGAGT TCAAAGTGCG CCCTTTCTTC 
    AATGAGTTCC AGGGCACCGA CAGCGACATC AAAGTGGGAA AGACCCTGGA GGAGGCTCCC 
    AGCGCTGCTC CCAGAGGCGT CACCGTAACA GGAAGTGGAG ACAACGGCAC AGCAGTCCTG 
    GTCGCCTGGC AACCGCCGCC AGAGGAGGAG CAGAACGGCG TTGTGCAGGA GTACAAGGTA 
    AAACCCAAAC ACAACAGACA AAGGGAACAC GAAAAAACTC AGCTTAATGA AGTGCTGGAG 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214702.1
    CDS228..2810
    Translation

    Target ORF information:

    RefSeq Version XM_017359213.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359213.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGTTCA GAGATTGCAA TTGCAGATTT TTTATACGGA ACTGTGTCCT GCTTATGGTT 
    TGGTATCAGG GGTATTGGGG CGCCACTATG TTGCAGATGG CCCGATACAC TTTACTGGAG 
    GACTCAAAGT CTGTATTACT TCCAACAGAC ACGGGGACTC AGGATGGGAC TAGCAATAGG 
    GCCACAGAGA CCCCCACTGC AGAACACGTT GACGACAACT CTCTGGGCTA CACAGGATCC 
    CGTCTGCGGC AGGAAGATTA CGCACCCCGT ATTGTTGAAC ACCCCTCTGA TCTGATCGTA 
    TCCAAAGGCG AACCGGCCAC CCTAAACTGC AAGGCTGAAG GACGTCCCAC GCCCACAGTG 
    GAGTGGTACA AAGATGGAGA ACGAGTTGAA ACCGACCGGG ACAATCCTCG ATCCCACCGC 
    ATGCTCCTGC CCACCGGATC TCTCTTCTTT CTCCGCATTG TTCACGGCCG GCGCAGCAAA 
    CCCGACGAAG GCTCCTACGT TTGTGTGGCA CGTAACTATC TGGGAGAAGC CGTCAGCCAC 
    AACGCCTCGC TGGAAGTAGC TATTCTCCGA GATGATTTCC GACAGAACCC TGTGGACGTG 
    ATGGTGGCAG CGGGGGAGCC AGCTGTAATG GAGTGCCAGC CGCCCCGAGG ACACCCAGAG 
    CCCACCATCT CCTGGAAGAA AGATGGGGTC AATATAGACG ACAGAGACGA GAGGATAACG 
    ATACGAGGAG GAAAGCTCAT GATCACCAAC ACCAGAAAAA CGGACGCGGG GAAGTACGTG 
    TGCGTGGGAA CCAACATGAT GGGGGAACGA GAGAGCGAGA TCGCGGAGTT GACTGTTCTC 
    GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT 
    GAGTTCAGAT GTGAAGCTCA AGGAGACCCC GTGCCCACCA TCCGATGGAG GAAAGAGGAC 
    GGAGAACTGC CCAAGGGAAG GTATGAGATC TGGGAGGACC ACACTCTCAA ACTGAAGAGG 
    CTGACTTCAG GAGACGCCGG TACTTACACC TGTCAGGCGG AGAACATGAT GGGCAAGACG 
    GAGGCCAGCG CCACCCTCAC CGTCCACGTT GTGTCTGTTC CTCCAGTGTT TGTGGTCCGA 
    CCCAGGAATC AGGTGGCGGG TATGGGCCGC ACCGTCACCT TTCAGTGTGA AGCCACAGGA 
    AGTCCTCAGC CTGCAATATT CTGGCAGAAA GAAGGCAGTC AGAATCTCCT GTTCTCGTCT 
    CAGCCTCCTC CTCCGTCCAG CAGATTCTCT GTGTCTCAGA CTGGAGATCT GACCATCACA 
    GCAGTTGAGC GTTCAGATGG AGGATATTAT AGCTGTCAGG CTCTCAACAT CGCTGGCAGT 
    GTGATCACCA AAGCCCTGCT GGAGGTTACA GACGTGATTT CAGACAGGCC TCCACCAATC 
    GTCCGTCAGG GTCCAACCAA CCAGACTGTG GCAGTGGACG GGACGGTGGT TCTGAGCTGC 
    CAAGCCACCG GGACACCGAT TCCTACCATC TTATGGAGGA AAGATGGAGT TCTGGTGTCC 
    ACACATGATT CCCGACTCAA GCAGCTGGAC ACTGGAGCCC TGCAGATTCG ATACGCAAAG 
    CTGGGCGACA CTGGCATGTA CACCTGCACG GCTTCCACCC CGAGCGGAGA GGCGTCCTGG 
    AAGGCCTACC TGGAGGTGCA AGAGTTTGGT GCTCCAGTTC AACCGGGCCG TCCCACAGAT 
    CCCAACCTCA TCCCCAGCGC CCCATCTAAA CCAGACGTCA CGGATGTCAG CCGCACCTCC 
    GTGTCTCTTT CCTGGAAGCC GAATCTGAAC GCTGGAGCCA CACCCACCTC ATACGTCATT 
    GAGGCCTTCA GCCATGCAGC AGGCAGCAGC TGGCAGACGC TCGCCGATCA TGTGAAGACG 
    GAAAGCTTCG TGCTTAAGGG TCTGAAGCCA AGTGCCGTCT ACCTGTTTCT GGTGAGAGCG 
    GCCAATGCGT ACGGCCTGAG CGACCCCAGT CCCATCAGCG ACGCTGTCAA AACACAAGAC 
    GTCCCACCAA CCAGCCAGGG AGTGGAGCAT GGCCAGATCC AGAGAGAACT GGGAGATGTC 
    GTCATTCACC TTCACAATCC CACAATTCTC TCATCGTCTT CCGTCAGAGT CCAGTGGACT 
    GTGGAGCAGC AGTCTCAGTA TATTCAGGGC TATAAGGTGA TGTACCGTCC GTCTCCAGAA 
    GGCGCTCCGC AGCGAGTGGA TTGGGCCATG TTTGAGGTCG GAGCCCCGGG GGAGCACAGC 
    GCTGTCGTCA CCCAGCTCAA GAAGGGCATC ACCTATGAGT TCAAAGTGCG CCCTTTCTTC 
    AATGAGTTCC AGGGCACCGA CAGCGACATC AAAGTGGGAA AGACCCTGGA GGAGGCTCCC 
    AGCGCTGCTC CCAGAGGCGT CACCGTAACA GGAAGTGGAG ACAACGGCAC AGCAGTCCTG 
    GTCGCCTGGC AACCGCCGCC AGAGGAGGAG CAGAACGGCG TTGTGCAGGA GTACAAGGTA 
    AAACCCAAAC ACAACAGACA AAGGGAACAC GAAAAAACTC AGCTTAATGA AGTGCTGGAG 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( NM_001365167.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352096.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID ODa159830
    Clone ID Related Accession (Same CDS sequence) NM_001365167.1
    Accession Version NM_001365167.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-18
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform 2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR626875.10, CU694228.9, CU695212.10, CU855934.6 and FP015879.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01032944.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAGGTCA CATGGTCAGG CGCAGAAGTC CTCATCATTT TCACCTTGCT GCAGTTTGTC 
    CATCCAGAGC TTGCTGAGGG TCCTTTGGCT CCAGACACGG GGACTCAGGA TGGGACTAGC 
    AATAGGGCCA CAGAGACCCC CACTGCAGAA CACGTTGACG ACAACTCTCT GGGCTACACA 
    GGATCCCGTC TGCGGCAGGA AGATTACGCA CCCCGTATTG TTGAACACCC CTCTGATCTG 
    ATCGTATCCA AAGGCGAACC GGCCACCCTA AACTGCAAGG CTGAAGGACG TCCCACGCCC 
    ACAGTGGAGT GGTACAAAGA TGGAGAACGA GTTGAAACCG ACCGGGACAA TCCTCGATCC 
    CACCGCATGC TCCTGCCCAC CGGATCTCTC TTCTTTCTCC GCATTGTTCA CGGCCGGCGC 
    AGCAAACCCG ACGAAGGCTC CTACGTTTGT GTGGCACGTA ACTATCTGGG AGAAGCCGTC 
    AGCCACAACG CCTCGCTGGA AGTAGCTATT CTCCGAGATG ATTTCCGACA GAACCCTGTG 
    GACGTGATGG TGGCAGCGGG GGAGCCAGCT GTAATGGAGT GCCAGCCGCC CCGAGGACAC 
    CCAGAGCCCA CCATCTCCTG GAAGAAAGAT GGGGTCAATA TAGACGACAG AGACGAGAGG 
    ATAACGATAC GAGGAGGAAA GCTCATGATC ACCAACACCA GAAAAACGGA CGCGGGGAAG 
    TACGTGTGCG TGGGAACCAA CATGATGGGG GAACGAGAGA GCGAGATCGC GGAGTTGACT 
    GTTCTCGAGA GGCCCAGTTT CTTGCGAAAG CCGAGCAGCC AGTCCGTCCT TGTGGATCAG 
    AGCGTTGAGT TCAGATGTGA AGCTCAAGGA GACCCCGTGC CCACCATCCG ATGGAGGAAA 
    GAGGACGGAG AACTGCCCAA GGGAAGGTAT GAGATCTGGG AGGACCACAC TCTCAAACTG 
    AAGAGGCTGA CTTCAGGAGA CGCCGGTACT TACACCTGTC AGGCGGAGAA CATGATGGGC 
    AAGACGGAGG CCAGCGCCAC CCTCACCGTC CACGTTCCTC CAGTGTTTGT GGTCCGACCC 
    AGGAATCAGG TGGCGGGTAT GGGCCGCACC GTCACCTTTC AGTGTGAAGC CACAGGAAGT 
    CCTCAGCCTG CAATATTCTG GCAGAAAGAA GGCAGTCAGA ATCTCCTGTT CTCGTCTCAG 
    CCTCCTCCTC CGTCCAGCAG ATTCTCTGTG TCTCAGACTG GAGATCTGAC CATCACAGCA 
    GTTGAGCGTT CAGATGGAGG ATATTATAGC TGTCAGGCTC TCAACATCGC TGGCAGTGTG 
    ATCACCAAAG CCCTGCTGGA GGTTACAGAC GTGATTTCAG ACAGGCCTCC ACCAATCGTC 
    CGTCAGGGTC CAACCAACCA GACTGTGGCA GTGGACGGGA CGGTGGTTCT GAGCTGCCAA 
    GCCACCGGGA CACCGATTCC TACCATCTTA TGGAGGAAAG ATGGAGTTCT GGTGTCCACA 
    CATGATTCCC GACTCAAGCA GCTGGACACT GGAGCCCTGC AGATTCGATA CGCAAAGCTG 
    GGCGACACTG GCATGTACAC CTGCACGGCT TCCACCCCGA GCGGAGAGGC GTCCTGGAAG 
    GCCTACCTGG AGGTGCAAGA GTTTGGTGCT CCAGTTCAAC CGGGCCGTCC CACAGATCCC 
    AACCTCATCC CCAGCGCCCC ATCTAAACCA GACGTCACGG ATGTCAGCCG CACCTCCGTG 
    TCTCTTTCCT GGAAGCCGAA TCTGAACGCT GGAGCCACAC CCACCTCATA CGTCATTGAG 
    GCCTTCAGCC ATGCAGCAGG CAGCAGCTGG CAGACGCTCG CCGATCATGT GAAGACGGAA 
    AGCTTCGTGC TTAAGGGTCT GAAGCCAAGT GCCGTCTACC TGTTTCTGGT GAGAGCGGCC 
    AATGCGTACG GCCTGAGCGA CCCCAGTCCC ATCAGCGACG CTGTCAAAAC ACAAGACGTC 
    CCACCAACCA GCCAGGGAGT GGAGCATGGC CAGATCCAGA GAGAACTGGG AGATGTCGTC 
    ATTCACCTTC ACAATCCCAC AATTCTCTCA TCGTCTTCCG TCAGAGTCCA GTGGACTGTG 
    GAGCAGCAGT CTCAGTATAT TCAGGGCTAT AAGGTGATGT ACCGTCCGTC TCCAGAAGGC 
    GCTCCGCAGC GAGTGGATTG GGCCATGTTT GAGGTCGGAG CCCCGGGGGA GCACAGCGCT 
    GTCGTCACCC AGCTCAAGAA GGGCATCACC TATGAGTTCA AAGTGCGCCC TTTCTTCAAT 
    GAGTTCCAGG GCACCGACAG CGACATCAAA GTGGGAAAGA CCCTGGAGGA GGCTCCCAGC 
    GCTGCTCCCA GAGGCGTCAC CGTAACAGGA AGTGGAGACA ACGGCACAGC AGTCCTGGTC 
    GCCTGGCAAC CGCCGCCAGA GGAGGAGCAG AACGGCGTTG TGCAGGAGTA CAAGATCTGG 
    TGTCTTGGTA ATGAAAGCCG ATACCACATC AACCGCACAG TGGATGGTTC TACACACTCG 
    GTGTTGATTC CTGGTCTGGT GGCAGGAGTC ACATACAGGC TAGAGGTGGC AGCAGGCACT 
    GGGGCCGGAC CCGGGGTCAA GAGCGACCCT ACGTCCTTCC AGCTTGACTC TTCTGGCCGT 
    GTTCTCCCGC CGCCGCTGGA CGACGATAAC ACCATTTCCC AGCAGATCTC AGACGTGGTG 
    CGTCAGCCGG CTTTCATCGC GGGCATCGGC GCCGCCTGCT GGATCATCCT GATGGTGTTC 
    AGCATCTGGC TATACCGCCA CCGCAAGAAG AGGAACGGCC TCAGCAGCAG CTACGCCGGC 
    ATCCGCAAAG TGCCCTCCTT CACCTTCACC CCCACAGTGG CCTATCAGAG AGGCGGAGAG 
    GCCGTCAGCA GTGCAGGAAG GCCGGGTTTG TTGAATATAG GTGAACCAGC TACACAGCCC 
    TGGCTTGCGG ACACCTGGCC CAACAGCTGC TCCAATCACA ATGACTGCAC TATTAACTGC 
    TGCACCGGCA GCAATGGAAA CAGCGACAGC AACCTGGCAA CTTACAGCCG CCCAGCCGAC 
    TGCATCGCCA ACTACAGCAA CCAGATGGAC AACAAGGCCA CCAACCTGCT GGCTCCAGAT 
    ACTGGGCTTT ACAGCGACGT TGACCTTTCA AACAAGATCA ACGAGATGAA GACCTTCAAC 
    AGCCCCAACC TGAAGGACGG GCGTTTTATG GGGCCTGGTG GTCAACCCAC ACCCTACGCC 
    ACCACTCAGC TCATTCAATC CAGCCTGGCC AACAACAACA TGAACAGCAG CAGTGGCGGA 
    AGCGGAGGAG GAAGCGCTGA CTTTAATGAG AAGCAGAGCT GGAAGAGCAG CCAGGGAACA 
    CCTCAGAAAC AGGCCGACAT CAACACACCA CTGCAGTATA ACGTCATGGA ACAAAACAAA 
    CTCAACAAGG ACCACTACCG AGGTGAGGGT CCCATCTCAG TCACCATCCC TTATAACCAG 
    GCCCAGGACG TCCTCACAGG AGAAACCTAC AGCAGCTCGG ACAGAGGGAG CAGCACCTCT 
    GGAAGTCAAG GGCAGAAAAA GGGTGGACGA ACAACTAAAC AAGCCAAACA GAATCCAATG 
    AATTGGACTG AACTGTTGCC TCCTCCTCCG GTTAATCCGC CGCCATGTCA AGAATACACC 
    CTGGTGATGG ACGACAGTTA CGACACAGAC CTGCAGTGCC CTTTGCCGCC GTCTCGGATG 
    TATCTGCAAC CTGACGAGTT GGAAGAGGAG GAGGACGAGG TGGAGCTGGA GCGTGGACCT 
    ACACCTCCTG TACGGGGAGC TGCTTCTTCA CCTGCTGCCG TGTCCTACAG CCACCAGTCC 
    ACCGCAACCC TAACCCCGTC CCCTCAGGAG GAGATGCATC CCATGCTGCA GGACAACCCT 
    GATCTACATG AGCGCAGGAG ACTGTTAGTG AGTCCACCTC CTCCACCACG ACCTCTCTCT 
    CCTCCACACA CATACGGCTA CATCTCCAGT CCCCTCGGCC TGGACACGGA CGGCCTAGAG 
    GAAGAGGATG ATGATGAAGA AGAGGGTGAC GAAACGGACG CAGAGGTAGC TCGAGTCCAC 
    CAGCACCACA TGATGCACCC TCACCAGCAG CACTTGCCCC AGCAGAGGGC TCTCCTGCGG 
    GGTCTGGAAC AAACCCCGGC CTCCAGCACC TGTGACCTGG AGAGCTCTGT GACTGGGTCT 
    ATGATTAACG GATGGGGTTC GGCTTCAGAA GAGGACAACG CCTCGTCAGG CCGTTCCAGT 
    GCAGTCAGCT CATCGGATGG ATCGTTCTTT ACCGATGCCG ATTTCGCCCA GGCTGTGGCA 
    GCTGCGGCTG AATATGCTGG TCTTCGAGTG GCAAAGCATC CTGGGAAAGG ACATCATCAG 
    CTTCCAGTGT CTGGAGCACG GAAATACCAA AACCTCCCTG CGGGACACCG TCCAGGCAGC 
    CCAGTGTCCA CAGACAGTAA CGTTAGCGCA GCCCCGGGTC AGAGGAGGCC CCTGAAGAGG 
    CAAAAACAAC ATCCAGCTGC TCAGACAGGC TACCAGCGCC GAGAGGCCTA CACAGAGGAT 
    CTCCCCCCTC CTCCGATTCC TCCTCCAGCC ATGCTGAAGT CTCCAACACA CCCGGCCAAA 
    ACCATCCCCG AGGGTCCACG GGGGGCCATG TCGCCAAGAT CCAGCTCTGC GGATGGCAAG 
    GACAGGCGAA CGGGTGCTGG CGGGCCCAGG GTCAGGGACG GTTCAGACAC TCGGACCAGC 
    TCTTCAGAGC GGAGGGATGG CCAAGATGTA CAGAGGGGTC AGAGAACGGG CAAGAATAGC 
    AAACAAGATG AAGCCGTGAA GAGCCGCCAG CACCCAGGCT CAGAGGACAT CCTACCTTAC 
    AGTCGTCCTT CTTTCCCAGC CGTCCACAGT CCTCGAGGAG AACGGGACCC CAGTTCCTCC 
    AGTTCACTGT CCTCTCGGGG GTCTGGCGGG CGACGGAGAG GTGAAGGGCC ACCAGGGGCC 
    CGCAGAAACC CTGCAGACAT TGGGCTAAAC ACCATAGGAG CCTTCCAGAC TGGAGGGGAC 
    GAGCTTGAGA TGGTGGAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352096.1
    CDS308..5371
    Translation

    Target ORF information:

    RefSeq Version NM_001365167.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001365167.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAGGTCA CATGGTCAGG CGCAGAAGTC CTCATCATTT TCACCTTGCT GCAGTTTGTC 
    CATCCAGAGC TTGCTGAGGG TCCTTTGGCT CCAGACACGG GGACTCAGGA TGGGACTAGC 
    AATAGGGCCA CAGAGACCCC CACTGCAGAA CACGTTGACG ACAACTCTCT GGGCTACACA 
    GGATCCCGTC TGCGGCAGGA AGATTACGCA CCCCGTATTG TTGAACACCC CTCTGATCTG 
    ATCGTATCCA AAGGCGAACC GGCCACCCTA AACTGCAAGG CTGAAGGACG TCCCACGCCC 
    ACAGTGGAGT GGTACAAAGA TGGAGAACGA GTTGAAACCG ACCGGGACAA TCCTCGATCC 
    CACCGCATGC TCCTGCCCAC CGGATCTCTC TTCTTTCTCC GCATTGTTCA CGGCCGGCGC 
    AGCAAACCCG ACGAAGGCTC CTACGTTTGT GTGGCACGTA ACTATCTGGG AGAAGCCGTC 
    AGCCACAACG CCTCGCTGGA AGTAGCTATT CTCCGAGATG ATTTCCGACA GAACCCTGTG 
    GACGTGATGG TGGCAGCGGG GGAGCCAGCT GTAATGGAGT GCCAGCCGCC CCGAGGACAC 
    CCAGAGCCCA CCATCTCCTG GAAGAAAGAT GGGGTCAATA TAGACGACAG AGACGAGAGG 
    ATAACGATAC GAGGAGGAAA GCTCATGATC ACCAACACCA GAAAAACGGA CGCGGGGAAG 
    TACGTGTGCG TGGGAACCAA CATGATGGGG GAACGAGAGA GCGAGATCGC GGAGTTGACT 
    GTTCTCGAGA GGCCCAGTTT CTTGCGAAAG CCGAGCAGCC AGTCCGTCCT TGTGGATCAG 
    AGCGTTGAGT TCAGATGTGA AGCTCAAGGA GACCCCGTGC CCACCATCCG ATGGAGGAAA 
    GAGGACGGAG AACTGCCCAA GGGAAGGTAT GAGATCTGGG AGGACCACAC TCTCAAACTG 
    AAGAGGCTGA CTTCAGGAGA CGCCGGTACT TACACCTGTC AGGCGGAGAA CATGATGGGC 
    AAGACGGAGG CCAGCGCCAC CCTCACCGTC CACGTTCCTC CAGTGTTTGT GGTCCGACCC 
    AGGAATCAGG TGGCGGGTAT GGGCCGCACC GTCACCTTTC AGTGTGAAGC CACAGGAAGT 
    CCTCAGCCTG CAATATTCTG GCAGAAAGAA GGCAGTCAGA ATCTCCTGTT CTCGTCTCAG 
    CCTCCTCCTC CGTCCAGCAG ATTCTCTGTG TCTCAGACTG GAGATCTGAC CATCACAGCA 
    GTTGAGCGTT CAGATGGAGG ATATTATAGC TGTCAGGCTC TCAACATCGC TGGCAGTGTG 
    ATCACCAAAG CCCTGCTGGA GGTTACAGAC GTGATTTCAG ACAGGCCTCC ACCAATCGTC 
    CGTCAGGGTC CAACCAACCA GACTGTGGCA GTGGACGGGA CGGTGGTTCT GAGCTGCCAA 
    GCCACCGGGA CACCGATTCC TACCATCTTA TGGAGGAAAG ATGGAGTTCT GGTGTCCACA 
    CATGATTCCC GACTCAAGCA GCTGGACACT GGAGCCCTGC AGATTCGATA CGCAAAGCTG 
    GGCGACACTG GCATGTACAC CTGCACGGCT TCCACCCCGA GCGGAGAGGC GTCCTGGAAG 
    GCCTACCTGG AGGTGCAAGA GTTTGGTGCT CCAGTTCAAC CGGGCCGTCC CACAGATCCC 
    AACCTCATCC CCAGCGCCCC ATCTAAACCA GACGTCACGG ATGTCAGCCG CACCTCCGTG 
    TCTCTTTCCT GGAAGCCGAA TCTGAACGCT GGAGCCACAC CCACCTCATA CGTCATTGAG 
    GCCTTCAGCC ATGCAGCAGG CAGCAGCTGG CAGACGCTCG CCGATCATGT GAAGACGGAA 
    AGCTTCGTGC TTAAGGGTCT GAAGCCAAGT GCCGTCTACC TGTTTCTGGT GAGAGCGGCC 
    AATGCGTACG GCCTGAGCGA CCCCAGTCCC ATCAGCGACG CTGTCAAAAC ACAAGACGTC 
    CCACCAACCA GCCAGGGAGT GGAGCATGGC CAGATCCAGA GAGAACTGGG AGATGTCGTC 
    ATTCACCTTC ACAATCCCAC AATTCTCTCA TCGTCTTCCG TCAGAGTCCA GTGGACTGTG 
    GAGCAGCAGT CTCAGTATAT TCAGGGCTAT AAGGTGATGT ACCGTCCGTC TCCAGAAGGC 
    GCTCCGCAGC GAGTGGATTG GGCCATGTTT GAGGTCGGAG CCCCGGGGGA GCACAGCGCT 
    GTCGTCACCC AGCTCAAGAA GGGCATCACC TATGAGTTCA AAGTGCGCCC TTTCTTCAAT 
    GAGTTCCAGG GCACCGACAG CGACATCAAA GTGGGAAAGA CCCTGGAGGA GGCTCCCAGC 
    GCTGCTCCCA GAGGCGTCAC CGTAACAGGA AGTGGAGACA ACGGCACAGC AGTCCTGGTC 
    GCCTGGCAAC CGCCGCCAGA GGAGGAGCAG AACGGCGTTG TGCAGGAGTA CAAGATCTGG 
    TGTCTTGGTA ATGAAAGCCG ATACCACATC AACCGCACAG TGGATGGTTC TACACACTCG 
    GTGTTGATTC CTGGTCTGGT GGCAGGAGTC ACATACAGGC TAGAGGTGGC AGCAGGCACT 
    GGGGCCGGAC CCGGGGTCAA GAGCGACCCT ACGTCCTTCC AGCTTGACTC TTCTGGCCGT 
    GTTCTCCCGC CGCCGCTGGA CGACGATAAC ACCATTTCCC AGCAGATCTC AGACGTGGTG 
    CGTCAGCCGG CTTTCATCGC GGGCATCGGC GCCGCCTGCT GGATCATCCT GATGGTGTTC 
    AGCATCTGGC TATACCGCCA CCGCAAGAAG AGGAACGGCC TCAGCAGCAG CTACGCCGGC 
    ATCCGCAAAG TGCCCTCCTT CACCTTCACC CCCACAGTGG CCTATCAGAG AGGCGGAGAG 
    GCCGTCAGCA GTGCAGGAAG GCCGGGTTTG TTGAATATAG GTGAACCAGC TACACAGCCC 
    TGGCTTGCGG ACACCTGGCC CAACAGCTGC TCCAATCACA ATGACTGCAC TATTAACTGC 
    TGCACCGGCA GCAATGGAAA CAGCGACAGC AACCTGGCAA CTTACAGCCG CCCAGCCGAC 
    TGCATCGCCA ACTACAGCAA CCAGATGGAC AACAAGGCCA CCAACCTGCT GGCTCCAGAT 
    ACTGGGCTTT ACAGCGACGT TGACCTTTCA AACAAGATCA ACGAGATGAA GACCTTCAAC 
    AGCCCCAACC TGAAGGACGG GCGTTTTATG GGGCCTGGTG GTCAACCCAC ACCCTACGCC 
    ACCACTCAGC TCATTCAATC CAGCCTGGCC AACAACAACA TGAACAGCAG CAGTGGCGGA 
    AGCGGAGGAG GAAGCGCTGA CTTTAATGAG AAGCAGAGCT GGAAGAGCAG CCAGGGAACA 
    CCTCAGAAAC AGGCCGACAT CAACACACCA CTGCAGTATA ACGTCATGGA ACAAAACAAA 
    CTCAACAAGG ACCACTACCG AGGTGAGGGT CCCATCTCAG TCACCATCCC TTATAACCAG 
    GCCCAGGACG TCCTCACAGG AGAAACCTAC AGCAGCTCGG ACAGAGGGAG CAGCACCTCT 
    GGAAGTCAAG GGCAGAAAAA GGGTGGACGA ACAACTAAAC AAGCCAAACA GAATCCAATG 
    AATTGGACTG AACTGTTGCC TCCTCCTCCG GTTAATCCGC CGCCATGTCA AGAATACACC 
    CTGGTGATGG ACGACAGTTA CGACACAGAC CTGCAGTGCC CTTTGCCGCC GTCTCGGATG 
    TATCTGCAAC CTGACGAGTT GGAAGAGGAG GAGGACGAGG TGGAGCTGGA GCGTGGACCT 
    ACACCTCCTG TACGGGGAGC TGCTTCTTCA CCTGCTGCCG TGTCCTACAG CCACCAGTCC 
    ACCGCAACCC TAACCCCGTC CCCTCAGGAG GAGATGCATC CCATGCTGCA GGACAACCCT 
    GATCTACATG AGCGCAGGAG ACTGTTAGTG AGTCCACCTC CTCCACCACG ACCTCTCTCT 
    CCTCCACACA CATACGGCTA CATCTCCAGT CCCCTCGGCC TGGACACGGA CGGCCTAGAG 
    GAAGAGGATG ATGATGAAGA AGAGGGTGAC GAAACGGACG CAGAGGTAGC TCGAGTCCAC 
    CAGCACCACA TGATGCACCC TCACCAGCAG CACTTGCCCC AGCAGAGGGC TCTCCTGCGG 
    GGTCTGGAAC AAACCCCGGC CTCCAGCACC TGTGACCTGG AGAGCTCTGT GACTGGGTCT 
    ATGATTAACG GATGGGGTTC GGCTTCAGAA GAGGACAACG CCTCGTCAGG CCGTTCCAGT 
    GCAGTCAGCT CATCGGATGG ATCGTTCTTT ACCGATGCCG ATTTCGCCCA GGCTGTGGCA 
    GCTGCGGCTG AATATGCTGG TCTTCGAGTG GCAAAGCATC CTGGGAAAGG ACATCATCAG 
    CTTCCAGTGT CTGGAGCACG GAAATACCAA AACCTCCCTG CGGGACACCG TCCAGGCAGC 
    CCAGTGTCCA CAGACAGTAA CGTTAGCGCA GCCCCGGGTC AGAGGAGGCC CCTGAAGAGG 
    CAAAAACAAC ATCCAGCTGC TCAGACAGGC TACCAGCGCC GAGAGGCCTA CACAGAGGAT 
    CTCCCCCCTC CTCCGATTCC TCCTCCAGCC ATGCTGAAGT CTCCAACACA CCCGGCCAAA 
    ACCATCCCCG AGGGTCCACG GGGGGCCATG TCGCCAAGAT CCAGCTCTGC GGATGGCAAG 
    GACAGGCGAA CGGGTGCTGG CGGGCCCAGG GTCAGGGACG GTTCAGACAC TCGGACCAGC 
    TCTTCAGAGC GGAGGGATGG CCAAGATGTA CAGAGGGGTC AGAGAACGGG CAAGAATAGC 
    AAACAAGATG AAGCCGTGAA GAGCCGCCAG CACCCAGGCT CAGAGGACAT CCTACCTTAC 
    AGTCGTCCTT CTTTCCCAGC CGTCCACAGT CCTCGAGGAG AACGGGACCC CAGTTCCTCC 
    AGTTCACTGT CCTCTCGGGG GTCTGGCGGG CGACGGAGAG GTGAAGGGCC ACCAGGGGCC 
    CGCAGAAACC CTGCAGACAT TGGGCTAAAC ACCATAGGAG CCTTCCAGAC TGGAGGGGAC 
    GAGCTTGAGA TGGTGGAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( NM_001365166.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352095.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID ODa55059
    Clone ID Related Accession (Same CDS sequence) XM_017359208.2 , XM_017359208.1 , NM_001365166.1
    Accession Version NM_001365166.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-18
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR626875.10, CU694228.9, CU695212.10, CU855934.6 and FP015879.3. On Aug 3, 2018 this sequence version replaced XM_017359208.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAGGTCA CATGGTCAGG CGCAGAAGTC CTCATCATTT TCACCTTGCT GCAGTTTGTC 
    CATCCAGAGC TTGCTGAGGG TCCTTTGGCT CCAGACACGG GGACTCAGGA TGGGACTAGC 
    AATAGGGCCA CAGAGACCCC CACTGCAGAA CACGTTGACG ACAACTCTCT GGGCTACACA 
    GGATCCCGTC TGCGGCAGGA AGATTACGCA CCCCGTATTG TTGAACACCC CTCTGATCTG 
    ATCGTATCCA AAGGCGAACC GGCCACCCTA AACTGCAAGG CTGAAGGACG TCCCACGCCC 
    ACAGTGGAGT GGTACAAAGA TGGAGAACGA GTTGAAACCG ACCGGGACAA TCCTCGATCC 
    CACCGCATGC TCCTGCCCAC CGGATCTCTC TTCTTTCTCC GCATTGTTCA CGGCCGGCGC 
    AGCAAACCCG ACGAAGGCTC CTACGTTTGT GTGGCACGTA ACTATCTGGG AGAAGCCGTC 
    AGCCACAACG CCTCGCTGGA AGTAGCTATT CTCCGAGATG ATTTCCGACA GAACCCTGTG 
    GACGTGATGG TGGCAGCGGG GGAGCCAGCT GTAATGGAGT GCCAGCCGCC CCGAGGACAC 
    CCAGAGCCCA CCATCTCCTG GAAGAAAGAT GGGGTCAATA TAGACGACAG AGACGAGAGG 
    ATAACGATAC GAGGAGGAAA GCTCATGATC ACCAACACCA GAAAAACGGA CGCGGGGAAG 
    TACGTGTGCG TGGGAACCAA CATGATGGGG GAACGAGAGA GCGAGATCGC GGAGTTGACT 
    GTTCTCGAGA GGCCCAGTTT CTTGCGAAAG CCGAGCAGCC AGTCCGTCCT TGTGGATCAG 
    AGCGTTGAGT TCAGATGTGA AGCTCAAGGA GACCCCGTGC CCACCATCCG ATGGAGGAAA 
    GAGGACGGAG AACTGCCCAA GGGAAGGTAT GAGATCTGGG AGGACCACAC TCTCAAACTG 
    AAGAGGCTGA CTTCAGGAGA CGCCGGTACT TACACCTGTC AGGCGGAGAA CATGATGGGC 
    AAGACGGAGG CCAGCGCCAC CCTCACCGTC CACGTTGTGT CTGTTCCTCC AGTGTTTGTG 
    GTCCGACCCA GGAATCAGGT GGCGGGTATG GGCCGCACCG TCACCTTTCA GTGTGAAGCC 
    ACAGGAAGTC CTCAGCCTGC AATATTCTGG CAGAAAGAAG GCAGTCAGAA TCTCCTGTTC 
    TCGTCTCAGC CTCCTCCTCC GTCCAGCAGA TTCTCTGTGT CTCAGACTGG AGATCTGACC 
    ATCACAGCAG TTGAGCGTTC AGATGGAGGA TATTATAGCT GTCAGGCTCT CAACATCGCT 
    GGCAGTGTGA TCACCAAAGC CCTGCTGGAG GTTACAGACG TGATTTCAGA CAGGCCTCCA 
    CCAATCGTCC GTCAGGGTCC AACCAACCAG ACTGTGGCAG TGGACGGGAC GGTGGTTCTG 
    AGCTGCCAAG CCACCGGGAC ACCGATTCCT ACCATCTTAT GGAGGAAAGA TGGAGTTCTG 
    GTGTCCACAC ATGATTCCCG ACTCAAGCAG CTGGACACTG GAGCCCTGCA GATTCGATAC 
    GCAAAGCTGG GCGACACTGG CATGTACACC TGCACGGCTT CCACCCCGAG CGGAGAGGCG 
    TCCTGGAAGG CCTACCTGGA GGTGCAAGAG TTTGGTGCTC CAGTTCAACC GGGCCGTCCC 
    ACAGATCCCA ACCTCATCCC CAGCGCCCCA TCTAAACCAG ACGTCACGGA TGTCAGCCGC 
    ACCTCCGTGT CTCTTTCCTG GAAGCCGAAT CTGAACGCTG GAGCCACACC CACCTCATAC 
    GTCATTGAGG CCTTCAGCCA TGCAGCAGGC AGCAGCTGGC AGACGCTCGC CGATCATGTG 
    AAGACGGAAA GCTTCGTGCT TAAGGGTCTG AAGCCAAGTG CCGTCTACCT GTTTCTGGTG 
    AGAGCGGCCA ATGCGTACGG CCTGAGCGAC CCCAGTCCCA TCAGCGACGC TGTCAAAACA 
    CAAGACGTCC CACCAACCAG CCAGGGAGTG GAGCATGGCC AGATCCAGAG AGAACTGGGA 
    GATGTCGTCA TTCACCTTCA CAATCCCACA ATTCTCTCAT CGTCTTCCGT CAGAGTCCAG 
    TGGACTGTGG AGCAGCAGTC TCAGTATATT CAGGGCTATA AGGTGATGTA CCGTCCGTCT 
    CCAGAAGGCG CTCCGCAGCG AGTGGATTGG GCCATGTTTG AGGTCGGAGC CCCGGGGGAG 
    CACAGCGCTG TCGTCACCCA GCTCAAGAAG GGCATCACCT ATGAGTTCAA AGTGCGCCCT 
    TTCTTCAATG AGTTCCAGGG CACCGACAGC GACATCAAAG TGGGAAAGAC CCTGGAGGAG 
    GCTCCCAGCG CTGCTCCCAG AGGCGTCACC GTAACAGGAA GTGGAGACAA CGGCACAGCA 
    GTCCTGGTCG CCTGGCAACC GCCGCCAGAG GAGGAGCAGA ACGGCGTTGT GCAGGAGTAC 
    AAGATCTGGT GTCTTGGTAA TGAAAGCCGA TACCACATCA ACCGCACAGT GGATGGTTCT 
    ACACACTCGG TGTTGATTCC TGGTCTGGTG GCAGGAGTCA CATACAGGCT AGAGGTGGCA 
    GCAGGCACTG GGGCCGGACC CGGGGTCAAG AGCGACCCTA CGTCCTTCCA GCTTGACTCT 
    TCTGGCCGTG TTCTCCCGCC GCCGCTGGAC GACGATAACA CCATTTCCCA GCAGATCTCA 
    GACGTGGTGC GTCAGCCGGC TTTCATCGCG GGCATCGGCG CCGCCTGCTG GATCATCCTG 
    ATGGTGTTCA GCATCTGGCT ATACCGCCAC CGCAAGAAGA GGAACGGCCT CAGCAGCAGC 
    TACGCCGGCA TCCGCAAAGT GCCCTCCTTC ACCTTCACCC CCACAGTGGC CTATCAGAGA 
    GGCGGAGAGG CCGTCAGCAG TGCAGGAAGG CCGGGTTTGT TGAATATAGG TGAACCAGCT 
    ACACAGCCCT GGCTTGCGGA CACCTGGCCC AACAGCTGCT CCAATCACAA TGACTGCACT 
    ATTAACTGCT GCACCGGCAG CAATGGAAAC AGCGACAGCA ACCTGGCAAC TTACAGCCGC 
    CCAGCCGACT GCATCGCCAA CTACAGCAAC CAGATGGACA ACAAGGCCAC CAACCTGCTG 
    GCTCCAGATA CTGGGCTTTA CAGCGACGTT GACCTTTCAA ACAAGATCAA CGAGATGAAG 
    ACCTTCAACA GCCCCAACCT GAAGGACGGG CGTTTTATGG GGCCTGGTGG TCAACCCACA 
    CCCTACGCCA CCACTCAGCT CATTCAATCC AGCCTGGCCA ACAACAACAT GAACAGCAGC 
    AGTGGCGGAA GCGGAGGAGG AAGCGCTGAC TTTAATGAGA AGCAGAGCTG GAAGAGCAGC 
    CAGGGAACAC CTCAGAAACA GGCCGACATC AACACACCAC TGCAGTATAA CGTCATGGAA 
    CAAAACAAAC TCAACAAGGA CCACTACCGA GGTGAGGGTC CCATCTCAGT CACCATCCCT 
    TATAACCAGG CCCAGGACGT CCTCACAGGA GAAACCTACA GCAGCTCGGA CAGAGGGAGC 
    AGCACCTCTG GAAGTCAAGG GCAGAAAAAG GGTGGACGAA CAACTAAACA AGCCAAACAG 
    AATCCAATGA ATTGGACTGA ACTGTTGCCT CCTCCTCCGG TTAATCCGCC GCCATGTCAA 
    GAATACACCC TGGTGATGGA CGACAGTTAC GACACAGACC TGCAGTGCCC TTTGCCGCCG 
    TCTCGGATGT ATCTGCAACC TGACGAGTTG GAAGAGGAGG AGGACGAGGT GGAGCTGGAG 
    CGTGGACCTA CACCTCCTGT ACGGGGAGCT GCTTCTTCAC CTGCTGCCGT GTCCTACAGC 
    CACCAGTCCA CCGCAACCCT AACCCCGTCC CCTCAGGAGG AGATGCATCC CATGCTGCAG 
    GACAACCCTG ATCTACATGA GCGCAGGAGA CTGTTAGTGA GTCCACCTCC TCCACCACGA 
    CCTCTCTCTC CTCCACACAC ATACGGCTAC ATCTCCAGTC CCCTCGGCCT GGACACGGAC 
    GGCCTAGAGG AAGAGGATGA TGATGAAGAA GAGGGTGACG AAACGGACGC AGAGGTAGCT 
    CGAGTCCACC AGCACCACAT GATGCACCCT CACCAGCAGC ACTTGCCCCA GCAGAGGGCT 
    CTCCTGCGGG GTCTGGAACA AACCCCGGCC TCCAGCACCT GTGACCTGGA GAGCTCTGTG 
    ACTGGGTCTA TGATTAACGG ATGGGGTTCG GCTTCAGAAG AGGACAACGC CTCGTCAGGC 
    CGTTCCAGTG CAGTCAGCTC ATCGGATGGA TCGTTCTTTA CCGATGCCGA TTTCGCCCAG 
    GCTGTGGCAG CTGCGGCTGA ATATGCTGGT CTTCGAGTGG CAAAGCATCC TGGGAAAGGA 
    CATCATCAGC TTCCAGTGTC TGGAGCAGCA CGGAAATACC AAAACCTCCC TGCGGGACAC 
    CGTCCAGGCA GCCCAGTGTC CACAGACAGT AACGTTAGCG CAGCCCCGGG TCAGAGGAGG 
    CCCCTGAAGA GGCAAAAACA ACATCCAGCT GCTCAGACAG GCTACCAGCG CCGAGAGGCC 
    TACACAGAGG ATCTCCCCCC TCCTCCGATT CCTCCTCCAG CCATGCTGAA GTCTCCAACA 
    CACCCGGCCA AAACCATCCC CGAGGGTCCA CGGGGGGCCA TGTCGCCAAG ATCCAGCTCT 
    GCGGATGGCA AGGACAGGCG AACGGGTGCT GGCGGGCCCA GGGTCAGGGA CGGTTCAGAC 
    ACTCGGACCA GCTCTTCAGA GCGGAGGGAT GGCCAAGATG TACAGAGGGG TCAGAGAACG 
    GGCAAGAATA GCAAACAAGA TGAAGCCGTG AAGAGCCGCC AGCACCCAGG CTCAGAGGAC 
    ATCCTACCTT ACAGTCGTCC TTCTTTCCCA GCCGTCCACA GTCCTCGAGG AGAACGGGAC 
    CCCAGTTCCT CCAGTTCACT GTCCTCTCGG GGGTCTGGCG GGCGACGGAG AGGTGAAGGG 
    CCACCAGGGG CCCGCAGAAA CCCTGCAGAC ATTGGGCTAA ACACCATAGG AGCCTTCCAG 
    ACTGGAGGGG ACGAGCTTGA GATGGTGGAG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352095.1
    CDS308..5383
    Translation

    Target ORF information:

    RefSeq Version NM_001365166.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001365166.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAGGTCA CATGGTCAGG CGCAGAAGTC CTCATCATTT TCACCTTGCT GCAGTTTGTC 
    CATCCAGAGC TTGCTGAGGG TCCTTTGGCT CCAGACACGG GGACTCAGGA TGGGACTAGC 
    AATAGGGCCA CAGAGACCCC CACTGCAGAA CACGTTGACG ACAACTCTCT GGGCTACACA 
    GGATCCCGTC TGCGGCAGGA AGATTACGCA CCCCGTATTG TTGAACACCC CTCTGATCTG 
    ATCGTATCCA AAGGCGAACC GGCCACCCTA AACTGCAAGG CTGAAGGACG TCCCACGCCC 
    ACAGTGGAGT GGTACAAAGA TGGAGAACGA GTTGAAACCG ACCGGGACAA TCCTCGATCC 
    CACCGCATGC TCCTGCCCAC CGGATCTCTC TTCTTTCTCC GCATTGTTCA CGGCCGGCGC 
    AGCAAACCCG ACGAAGGCTC CTACGTTTGT GTGGCACGTA ACTATCTGGG AGAAGCCGTC 
    AGCCACAACG CCTCGCTGGA AGTAGCTATT CTCCGAGATG ATTTCCGACA GAACCCTGTG 
    GACGTGATGG TGGCAGCGGG GGAGCCAGCT GTAATGGAGT GCCAGCCGCC CCGAGGACAC 
    CCAGAGCCCA CCATCTCCTG GAAGAAAGAT GGGGTCAATA TAGACGACAG AGACGAGAGG 
    ATAACGATAC GAGGAGGAAA GCTCATGATC ACCAACACCA GAAAAACGGA CGCGGGGAAG 
    TACGTGTGCG TGGGAACCAA CATGATGGGG GAACGAGAGA GCGAGATCGC GGAGTTGACT 
    GTTCTCGAGA GGCCCAGTTT CTTGCGAAAG CCGAGCAGCC AGTCCGTCCT TGTGGATCAG 
    AGCGTTGAGT TCAGATGTGA AGCTCAAGGA GACCCCGTGC CCACCATCCG ATGGAGGAAA 
    GAGGACGGAG AACTGCCCAA GGGAAGGTAT GAGATCTGGG AGGACCACAC TCTCAAACTG 
    AAGAGGCTGA CTTCAGGAGA CGCCGGTACT TACACCTGTC AGGCGGAGAA CATGATGGGC 
    AAGACGGAGG CCAGCGCCAC CCTCACCGTC CACGTTGTGT CTGTTCCTCC AGTGTTTGTG 
    GTCCGACCCA GGAATCAGGT GGCGGGTATG GGCCGCACCG TCACCTTTCA GTGTGAAGCC 
    ACAGGAAGTC CTCAGCCTGC AATATTCTGG CAGAAAGAAG GCAGTCAGAA TCTCCTGTTC 
    TCGTCTCAGC CTCCTCCTCC GTCCAGCAGA TTCTCTGTGT CTCAGACTGG AGATCTGACC 
    ATCACAGCAG TTGAGCGTTC AGATGGAGGA TATTATAGCT GTCAGGCTCT CAACATCGCT 
    GGCAGTGTGA TCACCAAAGC CCTGCTGGAG GTTACAGACG TGATTTCAGA CAGGCCTCCA 
    CCAATCGTCC GTCAGGGTCC AACCAACCAG ACTGTGGCAG TGGACGGGAC GGTGGTTCTG 
    AGCTGCCAAG CCACCGGGAC ACCGATTCCT ACCATCTTAT GGAGGAAAGA TGGAGTTCTG 
    GTGTCCACAC ATGATTCCCG ACTCAAGCAG CTGGACACTG GAGCCCTGCA GATTCGATAC 
    GCAAAGCTGG GCGACACTGG CATGTACACC TGCACGGCTT CCACCCCGAG CGGAGAGGCG 
    TCCTGGAAGG CCTACCTGGA GGTGCAAGAG TTTGGTGCTC CAGTTCAACC GGGCCGTCCC 
    ACAGATCCCA ACCTCATCCC CAGCGCCCCA TCTAAACCAG ACGTCACGGA TGTCAGCCGC 
    ACCTCCGTGT CTCTTTCCTG GAAGCCGAAT CTGAACGCTG GAGCCACACC CACCTCATAC 
    GTCATTGAGG CCTTCAGCCA TGCAGCAGGC AGCAGCTGGC AGACGCTCGC CGATCATGTG 
    AAGACGGAAA GCTTCGTGCT TAAGGGTCTG AAGCCAAGTG CCGTCTACCT GTTTCTGGTG 
    AGAGCGGCCA ATGCGTACGG CCTGAGCGAC CCCAGTCCCA TCAGCGACGC TGTCAAAACA 
    CAAGACGTCC CACCAACCAG CCAGGGAGTG GAGCATGGCC AGATCCAGAG AGAACTGGGA 
    GATGTCGTCA TTCACCTTCA CAATCCCACA ATTCTCTCAT CGTCTTCCGT CAGAGTCCAG 
    TGGACTGTGG AGCAGCAGTC TCAGTATATT CAGGGCTATA AGGTGATGTA CCGTCCGTCT 
    CCAGAAGGCG CTCCGCAGCG AGTGGATTGG GCCATGTTTG AGGTCGGAGC CCCGGGGGAG 
    CACAGCGCTG TCGTCACCCA GCTCAAGAAG GGCATCACCT ATGAGTTCAA AGTGCGCCCT 
    TTCTTCAATG AGTTCCAGGG CACCGACAGC GACATCAAAG TGGGAAAGAC CCTGGAGGAG 
    GCTCCCAGCG CTGCTCCCAG AGGCGTCACC GTAACAGGAA GTGGAGACAA CGGCACAGCA 
    GTCCTGGTCG CCTGGCAACC GCCGCCAGAG GAGGAGCAGA ACGGCGTTGT GCAGGAGTAC 
    AAGATCTGGT GTCTTGGTAA TGAAAGCCGA TACCACATCA ACCGCACAGT GGATGGTTCT 
    ACACACTCGG TGTTGATTCC TGGTCTGGTG GCAGGAGTCA CATACAGGCT AGAGGTGGCA 
    GCAGGCACTG GGGCCGGACC CGGGGTCAAG AGCGACCCTA CGTCCTTCCA GCTTGACTCT 
    TCTGGCCGTG TTCTCCCGCC GCCGCTGGAC GACGATAACA CCATTTCCCA GCAGATCTCA 
    GACGTGGTGC GTCAGCCGGC TTTCATCGCG GGCATCGGCG CCGCCTGCTG GATCATCCTG 
    ATGGTGTTCA GCATCTGGCT ATACCGCCAC CGCAAGAAGA GGAACGGCCT CAGCAGCAGC 
    TACGCCGGCA TCCGCAAAGT GCCCTCCTTC ACCTTCACCC CCACAGTGGC CTATCAGAGA 
    GGCGGAGAGG CCGTCAGCAG TGCAGGAAGG CCGGGTTTGT TGAATATAGG TGAACCAGCT 
    ACACAGCCCT GGCTTGCGGA CACCTGGCCC AACAGCTGCT CCAATCACAA TGACTGCACT 
    ATTAACTGCT GCACCGGCAG CAATGGAAAC AGCGACAGCA ACCTGGCAAC TTACAGCCGC 
    CCAGCCGACT GCATCGCCAA CTACAGCAAC CAGATGGACA ACAAGGCCAC CAACCTGCTG 
    GCTCCAGATA CTGGGCTTTA CAGCGACGTT GACCTTTCAA ACAAGATCAA CGAGATGAAG 
    ACCTTCAACA GCCCCAACCT GAAGGACGGG CGTTTTATGG GGCCTGGTGG TCAACCCACA 
    CCCTACGCCA CCACTCAGCT CATTCAATCC AGCCTGGCCA ACAACAACAT GAACAGCAGC 
    AGTGGCGGAA GCGGAGGAGG AAGCGCTGAC TTTAATGAGA AGCAGAGCTG GAAGAGCAGC 
    CAGGGAACAC CTCAGAAACA GGCCGACATC AACACACCAC TGCAGTATAA CGTCATGGAA 
    CAAAACAAAC TCAACAAGGA CCACTACCGA GGTGAGGGTC CCATCTCAGT CACCATCCCT 
    TATAACCAGG CCCAGGACGT CCTCACAGGA GAAACCTACA GCAGCTCGGA CAGAGGGAGC 
    AGCACCTCTG GAAGTCAAGG GCAGAAAAAG GGTGGACGAA CAACTAAACA AGCCAAACAG 
    AATCCAATGA ATTGGACTGA ACTGTTGCCT CCTCCTCCGG TTAATCCGCC GCCATGTCAA 
    GAATACACCC TGGTGATGGA CGACAGTTAC GACACAGACC TGCAGTGCCC TTTGCCGCCG 
    TCTCGGATGT ATCTGCAACC TGACGAGTTG GAAGAGGAGG AGGACGAGGT GGAGCTGGAG 
    CGTGGACCTA CACCTCCTGT ACGGGGAGCT GCTTCTTCAC CTGCTGCCGT GTCCTACAGC 
    CACCAGTCCA CCGCAACCCT AACCCCGTCC CCTCAGGAGG AGATGCATCC CATGCTGCAG 
    GACAACCCTG ATCTACATGA GCGCAGGAGA CTGTTAGTGA GTCCACCTCC TCCACCACGA 
    CCTCTCTCTC CTCCACACAC ATACGGCTAC ATCTCCAGTC CCCTCGGCCT GGACACGGAC 
    GGCCTAGAGG AAGAGGATGA TGATGAAGAA GAGGGTGACG AAACGGACGC AGAGGTAGCT 
    CGAGTCCACC AGCACCACAT GATGCACCCT CACCAGCAGC ACTTGCCCCA GCAGAGGGCT 
    CTCCTGCGGG GTCTGGAACA AACCCCGGCC TCCAGCACCT GTGACCTGGA GAGCTCTGTG 
    ACTGGGTCTA TGATTAACGG ATGGGGTTCG GCTTCAGAAG AGGACAACGC CTCGTCAGGC 
    CGTTCCAGTG CAGTCAGCTC ATCGGATGGA TCGTTCTTTA CCGATGCCGA TTTCGCCCAG 
    GCTGTGGCAG CTGCGGCTGA ATATGCTGGT CTTCGAGTGG CAAAGCATCC TGGGAAAGGA 
    CATCATCAGC TTCCAGTGTC TGGAGCAGCA CGGAAATACC AAAACCTCCC TGCGGGACAC 
    CGTCCAGGCA GCCCAGTGTC CACAGACAGT AACGTTAGCG CAGCCCCGGG TCAGAGGAGG 
    CCCCTGAAGA GGCAAAAACA ACATCCAGCT GCTCAGACAG GCTACCAGCG CCGAGAGGCC 
    TACACAGAGG ATCTCCCCCC TCCTCCGATT CCTCCTCCAG CCATGCTGAA GTCTCCAACA 
    CACCCGGCCA AAACCATCCC CGAGGGTCCA CGGGGGGCCA TGTCGCCAAG ATCCAGCTCT 
    GCGGATGGCA AGGACAGGCG AACGGGTGCT GGCGGGCCCA GGGTCAGGGA CGGTTCAGAC 
    ACTCGGACCA GCTCTTCAGA GCGGAGGGAT GGCCAAGATG TACAGAGGGG TCAGAGAACG 
    GGCAAGAATA GCAAACAAGA TGAAGCCGTG AAGAGCCGCC AGCACCCAGG CTCAGAGGAC 
    ATCCTACCTT ACAGTCGTCC TTCTTTCCCA GCCGTCCACA GTCCTCGAGG AGAACGGGAC 
    CCCAGTTCCT CCAGTTCACT GTCCTCTCGG GGGTCTGGCG GGCGACGGAG AGGTGAAGGG 
    CCACCAGGGG CCCGCAGAAA CCCTGCAGAC ATTGGGCTAA ACACCATAGG AGCCTTCCAG 
    ACTGGAGGGG ACGAGCTTGA GATGGTGGAG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359205.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214694.2 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa148914
    Clone ID Related Accession (Same CDS sequence) XM_017359205.2
    Accession Version XM_017359205.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359205.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTTGCAGA TGGCCCGATA CACTTTACTG GAGGACTCAA AGTCTGTATT ACTTCCAACA 
    GACACGGGGA CTCAGGATGG GACTAGCAAT AGGGCCACAG AGACCCCCAC TGCAGAACAC 
    GTTGACGACA ACTCTCTGGG CTACACAGGA TCCCGTCTGC GGCAGGAAGA TTACGCACCC 
    CGTATTGTTG AACACCCCTC TGATCTGATC GTATCCAAAG GCGAACCGGC CACCCTAAAC 
    TGCAAGGCTG AAGGACGTCC CACGCCCACA GTGGAGTGGT ACAAAGATGG AGAACGAGTT 
    GAAACCGACC GGGACAATCC TCGATCCCAC CGCATGCTCC TGCCCACCGG ATCTCTCTTC 
    TTTCTCCGCA TTGTTCACGG CCGGCGCAGC AAACCCGACG AAGGCTCCTA CGTTTGTGTG 
    GCACGTAACT ATCTGGGAGA AGCCGTCAGC CACAACGCCT CGCTGGAAGT AGCTATTCTC 
    CGAGATGATT TCCGACAGAA CCCTGTGGAC GTGATGGTGG CAGCGGGGGA GCCAGCTGTA 
    ATGGAGTGCC AGCCGCCCCG AGGACACCCA GAGCCCACCA TCTCCTGGAA GAAAGATGGG 
    GTCAATATAG ACGACAGAGA CGAGAGGATA ACGATACGAG GAGGAAAGCT CATGATCACC 
    AACACCAGAA AAACGGACGC GGGGAAGTAC GTGTGCGTGG GAACCAACAT GATGGGGGAA 
    CGAGAGAGCG AGATCGCGGA GTTGACTGTT CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG 
    AGCAGCCAGT CCGTCCTTGT GGATCAGAGC GTTGAGTTCA GATGTGAAGC TCAAGGAGAC 
    CCCGTGCCCA CCATCCGATG GAGGAAAGAG GACGGAGAAC TGCCCAAGGG AAGGTATGAG 
    ATCTGGGAGG ACCACACTCT CAAACTGAAG AGGCTGACTT CAGGAGACGC CGGTACTTAC 
    ACCTGTCAGG CGGAGAACAT GATGGGCAAG ACGGAGGCCA GCGCCACCCT CACCGTCCAC 
    GTTGTGTCTG TTCCTCCAGT GTTTGTGGTC CGACCCAGGA ATCAGGTGGC GGGTATGGGC 
    CGCACCGTCA CCTTTCAGTG TGAAGCCACA GGAAGTCCTC AGCCTGCAAT ATTCTGGCAG 
    AAAGAAGGCA GTCAGAATCT CCTGTTCTCG TCTCAGCCTC CTCCTCCGTC CAGCAGATTC 
    TCTGTGTCTC AGACTGGAGA TCTGACCATC ACAGCAGTTG AGCGTTCAGA TGGAGGATAT 
    TATAGCTGTC AGGCTCTCAA CATCGCTGGC AGTGTGATCA CCAAAGCCCT GCTGGAGGTT 
    ACAGACGTGA TTTCAGACAG GCCTCCACCA ATCGTCCGTC AGGGTCCAAC CAACCAGACT 
    GTGGCAGTGG ACGGGACGGT GGTTCTGAGC TGCCAAGCCA CCGGGACACC GATTCCTACC 
    ATCTTATGGA GGAAAGATGG AGTTCTGGTG TCCACACATG ATTCCCGACT CAAGCAGCTG 
    GACACTGGAG CCCTGCAGAT TCGATACGCA AAGCTGGGCG ACACTGGCAT GTACACCTGC 
    ACGGCTTCCA CCCCGAGCGG AGAGGCGTCC TGGAAGGCCT ACCTGGAGGT GCAAGAGTTT 
    GGTGCTCCAG TTCAACCGGG CCGTCCCACA GATCCCAACC TCATCCCCAG CGCCCCATCT 
    AAACCAGACG TCACGGATGT CAGCCGCACC TCCGTGTCTC TTTCCTGGAA GCCGAATCTG 
    AACGCTGGAG CCACACCCAC CTCATACGTC ATTGAGGCCT TCAGCCATGC AGCAGGCAGC 
    AGCTGGCAGA CGCTCGCCGA TCATGTGAAG ACGGAAAGCT TCGTGCTTAA GGGTCTGAAG 
    CCAAGTGCCG TCTACCTGTT TCTGGTGAGA GCGGCCAATG CGTACGGCCT GAGCGACCCC 
    AGTCCCATCA GCGACGCTGT CAAAACACAA GACGTCCCAC CAACCAGCCA GGGAGTGGAG 
    CATGGCCAGA TCCAGAGAGA ACTGGGAGAT GTCGTCATTC ACCTTCACAA TCCCACAATT 
    CTCTCATCGT CTTCCGTCAG AGTCCAGTGG ACTGTGGAGC AGCAGTCTCA GTATATTCAG 
    GGCTATAAGG TGATGTACCG TCCGTCTCCA GAAGGCGCTC CGCAGCGAGT GGATTGGGCC 
    ATGTTTGAGG TCGGAGCCCC GGGGGAGCAC AGCGCTGTCG TCACCCAGCT CAAGAAGGGC 
    ATCACCTATG AGTTCAAAGT GCGCCCTTTC TTCAATGAGT TCCAGGGCAC CGACAGCGAC 
    ATCAAAGTGG GAAAGACCCT GGAGGAGGCT CCCAGCGCTG CTCCCAGAGG CGTCACCGTA 
    ACAGGAAGTG GAGACAACGG CACAGCAGTC CTGGTCGCCT GGCAACCGCC GCCAGAGGAG 
    GAGCAGAACG GCGTTGTGCA GGAGTACAAG ATCTGGTGTC TTGGTAATGA AAGCCGATAC 
    CACATCAACC GCACAGTGGA TGGTTCTACA CACTCGGTGT TGATTCCTGG TCTGGTGGCA 
    GGAGTCACAT ACAGGCTAGA GGTGGCAGCA GGCACTGGGG CCGGACCCGG GGTCAAGAGC 
    GACCCTACGT CCTTCCAGCT TGACTCTTCT GGCCGTGTTC TCCCGCCGCC GCTGGACGAC 
    GATAACACCA TTTCCCAGCA GATCTCAGAC GTGGTGCGTC AGCCGGCTTT CATCGCGGGC 
    ATCGGCGCCG CCTGCTGGAT CATCCTGATG GTGTTCAGCA TCTGGCTATA CCGCCACCGC 
    AAGAAGAGGA ACGGCCTCAG CAGCAGCTAC GCCGGCATCC GCAAAGTGCC CTCCTTCACC 
    TTCACCCCCA CAGTGGCCTA TCAGAGAGGC GGAGAGGCCG TCAGCAGTGC AGGAAGGCCG 
    GGTTTGTTGA ATATAGGTGA ACCAGCTACA CAGCCCTGGC TTGCGGACAC CTGGCCCAAC 
    AGCTGCTCCA ATCACAATGA CTGCACTATT AACTGCTGCA CCGGCAGCAA TGGAAACAGC 
    GACAGCAACC TGGCAACTTA CAGCCGCCCA GCCGACTGCA TCGCCAACTA CAGCAACCAG 
    ATGGACAACA AGGCCACCAA CCTGCTGGCT CCAGATACTG GGCTTTACAG CGACGTTGAC 
    CTTTCAAACA AGATCAACGA GATGAAGACC TTCAACAGCC CCAACCTGAA GGACGGGCGT 
    TTTATGGGGC CTGGTGGTCA ACCCACACCC TACGCCACCA CTCAGCTCAT TCAATCCAGC 
    CTGGCCAACA ACAACATGAA CAGCAGCAGT GGCGGAAGCG GAGGAGGAAG CGCTGACTTT 
    AATGAGAAGC AGAGCTGGAA GAGCAGCCAG GGAACACCTC AGAAACAGGC CGACATCAAC 
    ACACCACTGC AGTATAACGT CATGGAACAA AACAAACTCA ACAAGGACCA CTACCGAGGT 
    GAGGGTCCCA TCTCAGTCAC CATCCCTTAT AACCAGGCCC AGGACGTCCT CACAGGAGAA 
    ACCTACAGCA GCTCGGACAG AGGGAGCAGC ACCTCTGGAA GTCAAGGGCA GAAAAAGGGT 
    GGACGAACAA CTAAACAAGC CAAACAGAAT CCAATGAATT GGACTGAACT GTTGCCTCCT 
    CCTCCGGTTA ATCCGCCGCC ATGTCAAGAA TACACCCTGG TGATGGACGA CAGTTACGAC 
    ACAGACCTGC AGTGCCCTTT GCCGCCGTCT CGGATGTATC TGCAACCTGA CGAGTTGGAA 
    GAGGAGGAGG ACGAGGTGGA GCTGGAGCGT GGACCTACAC CTCCTGTACG GGGAGCTGCT 
    TCTTCACCTG CTGCCGTGTC CTACAGCCAC CAGTCCACCG CAACCCTAAC CCCGTCCCCT 
    CAGGAGGAGA TGCATCCCAT GCTGCAGGAC AACCCTGATC TACATGAGCG CAGGAGACTG 
    TTAGTGAGTC CACCTCCTCC ACCACGACCT CTCTCTCCTC CACACACATA CGGCTACATC 
    TCCAGTCCCC TCGGCCTGGA CACGGACGGC CTAGAGGAAG AGGATGATGA TGAAGAAGAG 
    GGTGACGAAA CGGACGCAGA GGTAGCTCGA GTCCACCAGC ACCACATGAT GCACCCTCAC 
    CAGCAGCACT TGCCCCAGCA GAGGGCTCTC CTGCGGGGTC TGGAACAAAC CCCGGCCTCC 
    AGCACCTGTG ACCTGGAGAG CTCTGTGACT GGGTCTATGA TTAACGGATG GGGTTCGGCT 
    TCAGAAGAGG ACAACGCCTC GTCAGGCCGT TCCAGTGCAG TCAGCTCATC GGATGGATCG 
    TTCTTTACCG ATGCCGATTT CGCCCAGGCT GTGGCAGCTG CGGCTGAATA TGCTGGTCTT 
    CGAGTGGCAA AGCATCCTGG GAAAGGACAT CATCAGCTTC CAGTGTCTGG AGCACGGAAA 
    TACCAAAACC TCCCTGCGGG ACACCGTCCA GGCAGCCCAG TGTCCACAGA CAGTAACGTT 
    AGCGCAGCCC CGGGTCAGAG GAGGCCCCTG AAGAGGCAAA AACAACATCC AGCTGCTCAG 
    ACAGGCTACC AGCGCCGAGA GGCCTACACA GAGGATCTCC CCCCTCCTCC GATTCCTCCT 
    CCAGCCATGC TGAAGTCTCC AACACACCCG GCCAAAACCA TCCCCGAGGG TCCACGGGGG 
    GCCATGTCGC CAAGATCCAG CTCTGCGGAT GGCAAGGACA GGCGAACGGG TGCTGGCGGG 
    CCCAGGGTCA GGGACGGTTC AGACACTCGG ACCAGCTCTT CAGAGCGGAG GGATGGCCAA 
    GATGTACAGA GGGGTCAGAG AACGGGCAAG AATAGCAAAC AAGATGAAGC CGTGAAGAGC 
    CGCCAGCACC CAGGCTCAGA GGACATCCTA CCTTACAGTC GTCCTTCTTT CCCAGCCGTC 
    CACAGTCCTC GAGGAGAACG GGACCCCAGT TCCTCCAGTT CACTGTCCTC TCGGGGGTCT 
    GGCGGGCGAC GGAGAGGTGA AGGGCCACCA GGGGCCCGCA GAAACCCTGC AGACATTGGG 
    CTAAACACCA TAGGAGCCTT CCAGACTGGA GGGGACGAGC TTGAGATGGT GGAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214694.2
    CDS7..5046
    Translation

    Target ORF information:

    RefSeq Version XM_017359205.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359205.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTTGCAGA TGGCCCGATA CACTTTACTG GAGGACTCAA AGTCTGTATT ACTTCCAACA 
    GACACGGGGA CTCAGGATGG GACTAGCAAT AGGGCCACAG AGACCCCCAC TGCAGAACAC 
    GTTGACGACA ACTCTCTGGG CTACACAGGA TCCCGTCTGC GGCAGGAAGA TTACGCACCC 
    CGTATTGTTG AACACCCCTC TGATCTGATC GTATCCAAAG GCGAACCGGC CACCCTAAAC 
    TGCAAGGCTG AAGGACGTCC CACGCCCACA GTGGAGTGGT ACAAAGATGG AGAACGAGTT 
    GAAACCGACC GGGACAATCC TCGATCCCAC CGCATGCTCC TGCCCACCGG ATCTCTCTTC 
    TTTCTCCGCA TTGTTCACGG CCGGCGCAGC AAACCCGACG AAGGCTCCTA CGTTTGTGTG 
    GCACGTAACT ATCTGGGAGA AGCCGTCAGC CACAACGCCT CGCTGGAAGT AGCTATTCTC 
    CGAGATGATT TCCGACAGAA CCCTGTGGAC GTGATGGTGG CAGCGGGGGA GCCAGCTGTA 
    ATGGAGTGCC AGCCGCCCCG AGGACACCCA GAGCCCACCA TCTCCTGGAA GAAAGATGGG 
    GTCAATATAG ACGACAGAGA CGAGAGGATA ACGATACGAG GAGGAAAGCT CATGATCACC 
    AACACCAGAA AAACGGACGC GGGGAAGTAC GTGTGCGTGG GAACCAACAT GATGGGGGAA 
    CGAGAGAGCG AGATCGCGGA GTTGACTGTT CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG 
    AGCAGCCAGT CCGTCCTTGT GGATCAGAGC GTTGAGTTCA GATGTGAAGC TCAAGGAGAC 
    CCCGTGCCCA CCATCCGATG GAGGAAAGAG GACGGAGAAC TGCCCAAGGG AAGGTATGAG 
    ATCTGGGAGG ACCACACTCT CAAACTGAAG AGGCTGACTT CAGGAGACGC CGGTACTTAC 
    ACCTGTCAGG CGGAGAACAT GATGGGCAAG ACGGAGGCCA GCGCCACCCT CACCGTCCAC 
    GTTGTGTCTG TTCCTCCAGT GTTTGTGGTC CGACCCAGGA ATCAGGTGGC GGGTATGGGC 
    CGCACCGTCA CCTTTCAGTG TGAAGCCACA GGAAGTCCTC AGCCTGCAAT ATTCTGGCAG 
    AAAGAAGGCA GTCAGAATCT CCTGTTCTCG TCTCAGCCTC CTCCTCCGTC CAGCAGATTC 
    TCTGTGTCTC AGACTGGAGA TCTGACCATC ACAGCAGTTG AGCGTTCAGA TGGAGGATAT 
    TATAGCTGTC AGGCTCTCAA CATCGCTGGC AGTGTGATCA CCAAAGCCCT GCTGGAGGTT 
    ACAGACGTGA TTTCAGACAG GCCTCCACCA ATCGTCCGTC AGGGTCCAAC CAACCAGACT 
    GTGGCAGTGG ACGGGACGGT GGTTCTGAGC TGCCAAGCCA CCGGGACACC GATTCCTACC 
    ATCTTATGGA GGAAAGATGG AGTTCTGGTG TCCACACATG ATTCCCGACT CAAGCAGCTG 
    GACACTGGAG CCCTGCAGAT TCGATACGCA AAGCTGGGCG ACACTGGCAT GTACACCTGC 
    ACGGCTTCCA CCCCGAGCGG AGAGGCGTCC TGGAAGGCCT ACCTGGAGGT GCAAGAGTTT 
    GGTGCTCCAG TTCAACCGGG CCGTCCCACA GATCCCAACC TCATCCCCAG CGCCCCATCT 
    AAACCAGACG TCACGGATGT CAGCCGCACC TCCGTGTCTC TTTCCTGGAA GCCGAATCTG 
    AACGCTGGAG CCACACCCAC CTCATACGTC ATTGAGGCCT TCAGCCATGC AGCAGGCAGC 
    AGCTGGCAGA CGCTCGCCGA TCATGTGAAG ACGGAAAGCT TCGTGCTTAA GGGTCTGAAG 
    CCAAGTGCCG TCTACCTGTT TCTGGTGAGA GCGGCCAATG CGTACGGCCT GAGCGACCCC 
    AGTCCCATCA GCGACGCTGT CAAAACACAA GACGTCCCAC CAACCAGCCA GGGAGTGGAG 
    CATGGCCAGA TCCAGAGAGA ACTGGGAGAT GTCGTCATTC ACCTTCACAA TCCCACAATT 
    CTCTCATCGT CTTCCGTCAG AGTCCAGTGG ACTGTGGAGC AGCAGTCTCA GTATATTCAG 
    GGCTATAAGG TGATGTACCG TCCGTCTCCA GAAGGCGCTC CGCAGCGAGT GGATTGGGCC 
    ATGTTTGAGG TCGGAGCCCC GGGGGAGCAC AGCGCTGTCG TCACCCAGCT CAAGAAGGGC 
    ATCACCTATG AGTTCAAAGT GCGCCCTTTC TTCAATGAGT TCCAGGGCAC CGACAGCGAC 
    ATCAAAGTGG GAAAGACCCT GGAGGAGGCT CCCAGCGCTG CTCCCAGAGG CGTCACCGTA 
    ACAGGAAGTG GAGACAACGG CACAGCAGTC CTGGTCGCCT GGCAACCGCC GCCAGAGGAG 
    GAGCAGAACG GCGTTGTGCA GGAGTACAAG ATCTGGTGTC TTGGTAATGA AAGCCGATAC 
    CACATCAACC GCACAGTGGA TGGTTCTACA CACTCGGTGT TGATTCCTGG TCTGGTGGCA 
    GGAGTCACAT ACAGGCTAGA GGTGGCAGCA GGCACTGGGG CCGGACCCGG GGTCAAGAGC 
    GACCCTACGT CCTTCCAGCT TGACTCTTCT GGCCGTGTTC TCCCGCCGCC GCTGGACGAC 
    GATAACACCA TTTCCCAGCA GATCTCAGAC GTGGTGCGTC AGCCGGCTTT CATCGCGGGC 
    ATCGGCGCCG CCTGCTGGAT CATCCTGATG GTGTTCAGCA TCTGGCTATA CCGCCACCGC 
    AAGAAGAGGA ACGGCCTCAG CAGCAGCTAC GCCGGCATCC GCAAAGTGCC CTCCTTCACC 
    TTCACCCCCA CAGTGGCCTA TCAGAGAGGC GGAGAGGCCG TCAGCAGTGC AGGAAGGCCG 
    GGTTTGTTGA ATATAGGTGA ACCAGCTACA CAGCCCTGGC TTGCGGACAC CTGGCCCAAC 
    AGCTGCTCCA ATCACAATGA CTGCACTATT AACTGCTGCA CCGGCAGCAA TGGAAACAGC 
    GACAGCAACC TGGCAACTTA CAGCCGCCCA GCCGACTGCA TCGCCAACTA CAGCAACCAG 
    ATGGACAACA AGGCCACCAA CCTGCTGGCT CCAGATACTG GGCTTTACAG CGACGTTGAC 
    CTTTCAAACA AGATCAACGA GATGAAGACC TTCAACAGCC CCAACCTGAA GGACGGGCGT 
    TTTATGGGGC CTGGTGGTCA ACCCACACCC TACGCCACCA CTCAGCTCAT TCAATCCAGC 
    CTGGCCAACA ACAACATGAA CAGCAGCAGT GGCGGAAGCG GAGGAGGAAG CGCTGACTTT 
    AATGAGAAGC AGAGCTGGAA GAGCAGCCAG GGAACACCTC AGAAACAGGC CGACATCAAC 
    ACACCACTGC AGTATAACGT CATGGAACAA AACAAACTCA ACAAGGACCA CTACCGAGGT 
    GAGGGTCCCA TCTCAGTCAC CATCCCTTAT AACCAGGCCC AGGACGTCCT CACAGGAGAA 
    ACCTACAGCA GCTCGGACAG AGGGAGCAGC ACCTCTGGAA GTCAAGGGCA GAAAAAGGGT 
    GGACGAACAA CTAAACAAGC CAAACAGAAT CCAATGAATT GGACTGAACT GTTGCCTCCT 
    CCTCCGGTTA ATCCGCCGCC ATGTCAAGAA TACACCCTGG TGATGGACGA CAGTTACGAC 
    ACAGACCTGC AGTGCCCTTT GCCGCCGTCT CGGATGTATC TGCAACCTGA CGAGTTGGAA 
    GAGGAGGAGG ACGAGGTGGA GCTGGAGCGT GGACCTACAC CTCCTGTACG GGGAGCTGCT 
    TCTTCACCTG CTGCCGTGTC CTACAGCCAC CAGTCCACCG CAACCCTAAC CCCGTCCCCT 
    CAGGAGGAGA TGCATCCCAT GCTGCAGGAC AACCCTGATC TACATGAGCG CAGGAGACTG 
    TTAGTGAGTC CACCTCCTCC ACCACGACCT CTCTCTCCTC CACACACATA CGGCTACATC 
    TCCAGTCCCC TCGGCCTGGA CACGGACGGC CTAGAGGAAG AGGATGATGA TGAAGAAGAG 
    GGTGACGAAA CGGACGCAGA GGTAGCTCGA GTCCACCAGC ACCACATGAT GCACCCTCAC 
    CAGCAGCACT TGCCCCAGCA GAGGGCTCTC CTGCGGGGTC TGGAACAAAC CCCGGCCTCC 
    AGCACCTGTG ACCTGGAGAG CTCTGTGACT GGGTCTATGA TTAACGGATG GGGTTCGGCT 
    TCAGAAGAGG ACAACGCCTC GTCAGGCCGT TCCAGTGCAG TCAGCTCATC GGATGGATCG 
    TTCTTTACCG ATGCCGATTT CGCCCAGGCT GTGGCAGCTG CGGCTGAATA TGCTGGTCTT 
    CGAGTGGCAA AGCATCCTGG GAAAGGACAT CATCAGCTTC CAGTGTCTGG AGCACGGAAA 
    TACCAAAACC TCCCTGCGGG ACACCGTCCA GGCAGCCCAG TGTCCACAGA CAGTAACGTT 
    AGCGCAGCCC CGGGTCAGAG GAGGCCCCTG AAGAGGCAAA AACAACATCC AGCTGCTCAG 
    ACAGGCTACC AGCGCCGAGA GGCCTACACA GAGGATCTCC CCCCTCCTCC GATTCCTCCT 
    CCAGCCATGC TGAAGTCTCC AACACACCCG GCCAAAACCA TCCCCGAGGG TCCACGGGGG 
    GCCATGTCGC CAAGATCCAG CTCTGCGGAT GGCAAGGACA GGCGAACGGG TGCTGGCGGG 
    CCCAGGGTCA GGGACGGTTC AGACACTCGG ACCAGCTCTT CAGAGCGGAG GGATGGCCAA 
    GATGTACAGA GGGGTCAGAG AACGGGCAAG AATAGCAAAC AAGATGAAGC CGTGAAGAGC 
    CGCCAGCACC CAGGCTCAGA GGACATCCTA CCTTACAGTC GTCCTTCTTT CCCAGCCGTC 
    CACAGTCCTC GAGGAGAACG GGACCCCAGT TCCTCCAGTT CACTGTCCTC TCGGGGGTCT 
    GGCGGGCGAC GGAGAGGTGA AGGGCCACCA GGGGCCCGCA GAAACCCTGC AGACATTGGG 
    CTAAACACCA TAGGAGCCTT CCAGACTGGA GGGGACGAGC TTGAGATGGT GGAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359206.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214695.2 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa148915
    Clone ID Related Accession (Same CDS sequence) XM_017359206.2
    Accession Version XM_017359206.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTTGCAGA TGGCCCGATA CACTTTACTG GAGGACTCAA AGTCTGTATT ACTTCCAACA 
    GACACGGGGA CTCAGGATGG GACTAGCAAT AGGGCCACAG AGACCCCCAC TGCAGAACAC 
    GTTGACGACA ACTCTCTGGG CTACACAGGA TCCCGTCTGC GGCAGGAAGA TTACGCACCC 
    CGTATTGTTG AACACCCCTC TGATCTGATC GTATCCAAAG GCGAACCGGC CACCCTAAAC 
    TGCAAGGCTG AAGGACGTCC CACGCCCACA GTGGAGTGGT ACAAAGATGG AGAACGAGTT 
    GAAACCGACC GGGACAATCC TCGATCCCAC CGCATGCTCC TGCCCACCGG ATCTCTCTTC 
    TTTCTCCGCA TTGTTCACGG CCGGCGCAGC AAACCCGACG AAGGCTCCTA CGTTTGTGTG 
    GCACGTAACT ATCTGGGAGA AGCCGTCAGC CACAACGCCT CGCTGGAAGT AGCTATTCTC 
    CGAGATGATT TCCGACAGAA CCCTGTGGAC GTGATGGTGG CAGCGGGGGA GCCAGCTGTA 
    ATGGAGTGCC AGCCGCCCCG AGGACACCCA GAGCCCACCA TCTCCTGGAA GAAAGATGGG 
    GTCAATATAG ACGACAGAGA CGAGAGGATA ACGATACGAG GAGGAAAGCT CATGATCACC 
    AACACCAGAA AAACGGACGC GGGGAAGTAC GTGTGCGTGG GAACCAACAT GATGGGGGAA 
    CGAGAGAGCG AGATCGCGGA GTTGACTGTT CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG 
    AGCAGCCAGT CCGTCCTTGT GGATCAGAGC GTTGAGTTCA GATGTGAAGC TCAAGGAGAC 
    CCCGTGCCCA CCATCCGATG GAGGAAAGAG GACGGAGAAC TGCCCAAGGG AAGGTATGAG 
    ATCTGGGAGG ACCACACTCT CAAACTGAAG AGGCTGACTT CAGGAGACGC CGGTACTTAC 
    ACCTGTCAGG CGGAGAACAT GATGGGCAAG ACGGAGGCCA GCGCCACCCT CACCGTCCAC 
    GTTCCTCCAG TGTTTGTGGT CCGACCCAGG AATCAGGTGG CGGGTATGGG CCGCACCGTC 
    ACCTTTCAGT GTGAAGCCAC AGGAAGTCCT CAGCCTGCAA TATTCTGGCA GAAAGAAGGC 
    AGTCAGAATC TCCTGTTCTC GTCTCAGCCT CCTCCTCCGT CCAGCAGATT CTCTGTGTCT 
    CAGACTGGAG ATCTGACCAT CACAGCAGTT GAGCGTTCAG ATGGAGGATA TTATAGCTGT 
    CAGGCTCTCA ACATCGCTGG CAGTGTGATC ACCAAAGCCC TGCTGGAGGT TACAGACGTG 
    ATTTCAGACA GGCCTCCACC AATCGTCCGT CAGGGTCCAA CCAACCAGAC TGTGGCAGTG 
    GACGGGACGG TGGTTCTGAG CTGCCAAGCC ACCGGGACAC CGATTCCTAC CATCTTATGG 
    AGGAAAGATG GAGTTCTGGT GTCCACACAT GATTCCCGAC TCAAGCAGCT GGACACTGGA 
    GCCCTGCAGA TTCGATACGC AAAGCTGGGC GACACTGGCA TGTACACCTG CACGGCTTCC 
    ACCCCGAGCG GAGAGGCGTC CTGGAAGGCC TACCTGGAGG TGCAAGAGTT TGGTGCTCCA 
    GTTCAACCGG GCCGTCCCAC AGATCCCAAC CTCATCCCCA GCGCCCCATC TAAACCAGAC 
    GTCACGGATG TCAGCCGCAC CTCCGTGTCT CTTTCCTGGA AGCCGAATCT GAACGCTGGA 
    GCCACACCCA CCTCATACGT CATTGAGGCC TTCAGCCATG CAGCAGGCAG CAGCTGGCAG 
    ACGCTCGCCG ATCATGTGAA GACGGAAAGC TTCGTGCTTA AGGGTCTGAA GCCAAGTGCC 
    GTCTACCTGT TTCTGGTGAG AGCGGCCAAT GCGTACGGCC TGAGCGACCC CAGTCCCATC 
    AGCGACGCTG TCAAAACACA AGACGTCCCA CCAACCAGCC AGGGAGTGGA GCATGGCCAG 
    ATCCAGAGAG AACTGGGAGA TGTCGTCATT CACCTTCACA ATCCCACAAT TCTCTCATCG 
    TCTTCCGTCA GAGTCCAGTG GACTGTGGAG CAGCAGTCTC AGTATATTCA GGGCTATAAG 
    GTGATGTACC GTCCGTCTCC AGAAGGCGCT CCGCAGCGAG TGGATTGGGC CATGTTTGAG 
    GTCGGAGCCC CGGGGGAGCA CAGCGCTGTC GTCACCCAGC TCAAGAAGGG CATCACCTAT 
    GAGTTCAAAG TGCGCCCTTT CTTCAATGAG TTCCAGGGCA CCGACAGCGA CATCAAAGTG 
    GGAAAGACCC TGGAGGAGGC TCCCAGCGCT GCTCCCAGAG GCGTCACCGT AACAGGAAGT 
    GGAGACAACG GCACAGCAGT CCTGGTCGCC TGGCAACCGC CGCCAGAGGA GGAGCAGAAC 
    GGCGTTGTGC AGGAGTACAA GATCTGGTGT CTTGGTAATG AAAGCCGATA CCACATCAAC 
    CGCACAGTGG ATGGTTCTAC ACACTCGGTG TTGATTCCTG GTCTGGTGGC AGGAGTCACA 
    TACAGGCTAG AGGTGGCAGC AGGCACTGGG GCCGGACCCG GGGTCAAGAG CGACCCTACG 
    TCCTTCCAGC TTGACTCTTC TGGCCGTGTT CTCCCGCCGC CGCTGGACGA CGATAACACC 
    ATTTCCCAGC AGATCTCAGA CGTGGTGCGT CAGCCGGCTT TCATCGCGGG CATCGGCGCC 
    GCCTGCTGGA TCATCCTGAT GGTGTTCAGC ATCTGGCTAT ACCGCCACCG CAAGAAGAGG 
    AACGGCCTCA GCAGCAGCTA CGCCGGCATC CGCAAAGTGC CCTCCTTCAC CTTCACCCCC 
    ACAGTGGCCT ATCAGAGAGG CGGAGAGGCC GTCAGCAGTG CAGGAAGGCC GGGTTTGTTG 
    AATATAGGTG AACCAGCTAC ACAGCCCTGG CTTGCGGACA CCTGGCCCAA CAGCTGCTCC 
    AATCACAATG ACTGCACTAT TAACTGCTGC ACCGGCAGCA ATGGAAACAG CGACAGCAAC 
    CTGGCAACTT ACAGCCGCCC AGCCGACTGC ATCGCCAACT ACAGCAACCA GATGGACAAC 
    AAGGCCACCA ACCTGCTGGC TCCAGATACT GGGCTTTACA GCGACGTTGA CCTTTCAAAC 
    AAGATCAACG AGATGAAGAC CTTCAACAGC CCCAACCTGA AGGACGGGCG TTTTATGGGG 
    CCTGGTGGTC AACCCACACC CTACGCCACC ACTCAGCTCA TTCAATCCAG CCTGGCCAAC 
    AACAACATGA ACAGCAGCAG TGGCGGAAGC GGAGGAGGAA GCGCTGACTT TAATGAGAAG 
    CAGAGCTGGA AGAGCAGCCA GGGAACACCT CAGAAACAGG CCGACATCAA CACACCACTG 
    CAGTATAACG TCATGGAACA AAACAAACTC AACAAGGACC ACTACCGAGG TGAGGGTCCC 
    ATCTCAGTCA CCATCCCTTA TAACCAGGCC CAGGACGTCC TCACAGGAGA AACCTACAGC 
    AGCTCGGACA GAGGGAGCAG CACCTCTGGA AGTCAAGGGC AGAAAAAGGG TGGACGAACA 
    ACTAAACAAG CCAAACAGAA TCCAATGAAT TGGACTGAAC TGTTGCCTCC TCCTCCGGTT 
    AATCCGCCGC CATGTCAAGA ATACACCCTG GTGATGGACG ACAGTTACGA CACAGACCTG 
    CAGTGCCCTT TGCCGCCGTC TCGGATGTAT CTGCAACCTG ACGAGTTGGA AGAGGAGGAG 
    GACGAGGTGG AGCTGGAGCG TGGACCTACA CCTCCTGTAC GGGGAGCTGC TTCTTCACCT 
    GCTGCCGTGT CCTACAGCCA CCAGTCCACC GCAACCCTAA CCCCGTCCCC TCAGGAGGAG 
    ATGCATCCCA TGCTGCAGGA CAACCCTGAT CTACATGAGC GCAGGAGACT GTTAGTGAGT 
    CCACCTCCTC CACCACGACC TCTCTCTCCT CCACACACAT ACGGCTACAT CTCCAGTCCC 
    CTCGGCCTGG ACACGGACGG CCTAGAGGAA GAGGATGATG ATGAAGAAGA GGGTGACGAA 
    ACGGACGCAG AGGTAGCTCG AGTCCACCAG CACCACATGA TGCACCCTCA CCAGCAGCAC 
    TTGCCCCAGC AGAGGGCTCT CCTGCGGGGT CTGGAACAAA CCCCGGCCTC CAGCACCTGT 
    GACCTGGAGA GCTCTGTGAC TGGGTCTATG ATTAACGGAT GGGGTTCGGC TTCAGAAGAG 
    GACAACGCCT CGTCAGGCCG TTCCAGTGCA GTCAGCTCAT CGGATGGATC GTTCTTTACC 
    GATGCCGATT TCGCCCAGGC TGTGGCAGCT GCGGCTGAAT ATGCTGGTCT TCGAGTGGCA 
    AAGCATCCTG GGAAAGGACA TCATCAGCTT CCAGTGTCTG GAGCAGCACG GAAATACCAA 
    AACCTCCCTG CGGGACACCG TCCAGGCAGC CCAGTGTCCA CAGACAGTAA CGTTAGCGCA 
    GCCCCGGGTC AGAGGAGGCC CCTGAAGAGG CAAAAACAAC ATCCAGCTGC TCAGACAGGC 
    TACCAGCGCC GAGAGGCCTA CACAGAGGAT CTCCCCCCTC CTCCGATTCC TCCTCCAGCC 
    ATGCTGAAGT CTCCAACACA CCCGGCCAAA ACCATCCCCG AGGGTCCACG GGGGGCCATG 
    TCGCCAAGAT CCAGCTCTGC GGATGGCAAG GACAGGCGAA CGGGTGCTGG CGGGCCCAGG 
    GTCAGGGACG GTTCAGACAC TCGGACCAGC TCTTCAGAGC GGAGGGATGG CCAAGATGTA 
    CAGAGGGGTC AGAGAACGGG CAAGAATAGC AAACAAGATG AAGCCGTGAA GAGCCGCCAG 
    CACCCAGGCT CAGAGGACAT CCTACCTTAC AGTCGTCCTT CTTTCCCAGC CGTCCACAGT 
    CCTCGAGGAG AACGGGACCC CAGTTCCTCC AGTTCACTGT CCTCTCGGGG GTCTGGCGGG 
    CGACGGAGAG GTGAAGGGCC ACCAGGGGCC CGCAGAAACC CTGCAGACAT TGGGCTAAAC 
    ACCATAGGAG CCTTCCAGAC TGGAGGGGAC GAGCTTGAGA TGGTGGAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214695.2
    CDS7..5040
    Translation

    Target ORF information:

    RefSeq Version XM_017359206.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359206.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTTGCAGA TGGCCCGATA CACTTTACTG GAGGACTCAA AGTCTGTATT ACTTCCAACA 
    GACACGGGGA CTCAGGATGG GACTAGCAAT AGGGCCACAG AGACCCCCAC TGCAGAACAC 
    GTTGACGACA ACTCTCTGGG CTACACAGGA TCCCGTCTGC GGCAGGAAGA TTACGCACCC 
    CGTATTGTTG AACACCCCTC TGATCTGATC GTATCCAAAG GCGAACCGGC CACCCTAAAC 
    TGCAAGGCTG AAGGACGTCC CACGCCCACA GTGGAGTGGT ACAAAGATGG AGAACGAGTT 
    GAAACCGACC GGGACAATCC TCGATCCCAC CGCATGCTCC TGCCCACCGG ATCTCTCTTC 
    TTTCTCCGCA TTGTTCACGG CCGGCGCAGC AAACCCGACG AAGGCTCCTA CGTTTGTGTG 
    GCACGTAACT ATCTGGGAGA AGCCGTCAGC CACAACGCCT CGCTGGAAGT AGCTATTCTC 
    CGAGATGATT TCCGACAGAA CCCTGTGGAC GTGATGGTGG CAGCGGGGGA GCCAGCTGTA 
    ATGGAGTGCC AGCCGCCCCG AGGACACCCA GAGCCCACCA TCTCCTGGAA GAAAGATGGG 
    GTCAATATAG ACGACAGAGA CGAGAGGATA ACGATACGAG GAGGAAAGCT CATGATCACC 
    AACACCAGAA AAACGGACGC GGGGAAGTAC GTGTGCGTGG GAACCAACAT GATGGGGGAA 
    CGAGAGAGCG AGATCGCGGA GTTGACTGTT CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG 
    AGCAGCCAGT CCGTCCTTGT GGATCAGAGC GTTGAGTTCA GATGTGAAGC TCAAGGAGAC 
    CCCGTGCCCA CCATCCGATG GAGGAAAGAG GACGGAGAAC TGCCCAAGGG AAGGTATGAG 
    ATCTGGGAGG ACCACACTCT CAAACTGAAG AGGCTGACTT CAGGAGACGC CGGTACTTAC 
    ACCTGTCAGG CGGAGAACAT GATGGGCAAG ACGGAGGCCA GCGCCACCCT CACCGTCCAC 
    GTTCCTCCAG TGTTTGTGGT CCGACCCAGG AATCAGGTGG CGGGTATGGG CCGCACCGTC 
    ACCTTTCAGT GTGAAGCCAC AGGAAGTCCT CAGCCTGCAA TATTCTGGCA GAAAGAAGGC 
    AGTCAGAATC TCCTGTTCTC GTCTCAGCCT CCTCCTCCGT CCAGCAGATT CTCTGTGTCT 
    CAGACTGGAG ATCTGACCAT CACAGCAGTT GAGCGTTCAG ATGGAGGATA TTATAGCTGT 
    CAGGCTCTCA ACATCGCTGG CAGTGTGATC ACCAAAGCCC TGCTGGAGGT TACAGACGTG 
    ATTTCAGACA GGCCTCCACC AATCGTCCGT CAGGGTCCAA CCAACCAGAC TGTGGCAGTG 
    GACGGGACGG TGGTTCTGAG CTGCCAAGCC ACCGGGACAC CGATTCCTAC CATCTTATGG 
    AGGAAAGATG GAGTTCTGGT GTCCACACAT GATTCCCGAC TCAAGCAGCT GGACACTGGA 
    GCCCTGCAGA TTCGATACGC AAAGCTGGGC GACACTGGCA TGTACACCTG CACGGCTTCC 
    ACCCCGAGCG GAGAGGCGTC CTGGAAGGCC TACCTGGAGG TGCAAGAGTT TGGTGCTCCA 
    GTTCAACCGG GCCGTCCCAC AGATCCCAAC CTCATCCCCA GCGCCCCATC TAAACCAGAC 
    GTCACGGATG TCAGCCGCAC CTCCGTGTCT CTTTCCTGGA AGCCGAATCT GAACGCTGGA 
    GCCACACCCA CCTCATACGT CATTGAGGCC TTCAGCCATG CAGCAGGCAG CAGCTGGCAG 
    ACGCTCGCCG ATCATGTGAA GACGGAAAGC TTCGTGCTTA AGGGTCTGAA GCCAAGTGCC 
    GTCTACCTGT TTCTGGTGAG AGCGGCCAAT GCGTACGGCC TGAGCGACCC CAGTCCCATC 
    AGCGACGCTG TCAAAACACA AGACGTCCCA CCAACCAGCC AGGGAGTGGA GCATGGCCAG 
    ATCCAGAGAG AACTGGGAGA TGTCGTCATT CACCTTCACA ATCCCACAAT TCTCTCATCG 
    TCTTCCGTCA GAGTCCAGTG GACTGTGGAG CAGCAGTCTC AGTATATTCA GGGCTATAAG 
    GTGATGTACC GTCCGTCTCC AGAAGGCGCT CCGCAGCGAG TGGATTGGGC CATGTTTGAG 
    GTCGGAGCCC CGGGGGAGCA CAGCGCTGTC GTCACCCAGC TCAAGAAGGG CATCACCTAT 
    GAGTTCAAAG TGCGCCCTTT CTTCAATGAG TTCCAGGGCA CCGACAGCGA CATCAAAGTG 
    GGAAAGACCC TGGAGGAGGC TCCCAGCGCT GCTCCCAGAG GCGTCACCGT AACAGGAAGT 
    GGAGACAACG GCACAGCAGT CCTGGTCGCC TGGCAACCGC CGCCAGAGGA GGAGCAGAAC 
    GGCGTTGTGC AGGAGTACAA GATCTGGTGT CTTGGTAATG AAAGCCGATA CCACATCAAC 
    CGCACAGTGG ATGGTTCTAC ACACTCGGTG TTGATTCCTG GTCTGGTGGC AGGAGTCACA 
    TACAGGCTAG AGGTGGCAGC AGGCACTGGG GCCGGACCCG GGGTCAAGAG CGACCCTACG 
    TCCTTCCAGC TTGACTCTTC TGGCCGTGTT CTCCCGCCGC CGCTGGACGA CGATAACACC 
    ATTTCCCAGC AGATCTCAGA CGTGGTGCGT CAGCCGGCTT TCATCGCGGG CATCGGCGCC 
    GCCTGCTGGA TCATCCTGAT GGTGTTCAGC ATCTGGCTAT ACCGCCACCG CAAGAAGAGG 
    AACGGCCTCA GCAGCAGCTA CGCCGGCATC CGCAAAGTGC CCTCCTTCAC CTTCACCCCC 
    ACAGTGGCCT ATCAGAGAGG CGGAGAGGCC GTCAGCAGTG CAGGAAGGCC GGGTTTGTTG 
    AATATAGGTG AACCAGCTAC ACAGCCCTGG CTTGCGGACA CCTGGCCCAA CAGCTGCTCC 
    AATCACAATG ACTGCACTAT TAACTGCTGC ACCGGCAGCA ATGGAAACAG CGACAGCAAC 
    CTGGCAACTT ACAGCCGCCC AGCCGACTGC ATCGCCAACT ACAGCAACCA GATGGACAAC 
    AAGGCCACCA ACCTGCTGGC TCCAGATACT GGGCTTTACA GCGACGTTGA CCTTTCAAAC 
    AAGATCAACG AGATGAAGAC CTTCAACAGC CCCAACCTGA AGGACGGGCG TTTTATGGGG 
    CCTGGTGGTC AACCCACACC CTACGCCACC ACTCAGCTCA TTCAATCCAG CCTGGCCAAC 
    AACAACATGA ACAGCAGCAG TGGCGGAAGC GGAGGAGGAA GCGCTGACTT TAATGAGAAG 
    CAGAGCTGGA AGAGCAGCCA GGGAACACCT CAGAAACAGG CCGACATCAA CACACCACTG 
    CAGTATAACG TCATGGAACA AAACAAACTC AACAAGGACC ACTACCGAGG TGAGGGTCCC 
    ATCTCAGTCA CCATCCCTTA TAACCAGGCC CAGGACGTCC TCACAGGAGA AACCTACAGC 
    AGCTCGGACA GAGGGAGCAG CACCTCTGGA AGTCAAGGGC AGAAAAAGGG TGGACGAACA 
    ACTAAACAAG CCAAACAGAA TCCAATGAAT TGGACTGAAC TGTTGCCTCC TCCTCCGGTT 
    AATCCGCCGC CATGTCAAGA ATACACCCTG GTGATGGACG ACAGTTACGA CACAGACCTG 
    CAGTGCCCTT TGCCGCCGTC TCGGATGTAT CTGCAACCTG ACGAGTTGGA AGAGGAGGAG 
    GACGAGGTGG AGCTGGAGCG TGGACCTACA CCTCCTGTAC GGGGAGCTGC TTCTTCACCT 
    GCTGCCGTGT CCTACAGCCA CCAGTCCACC GCAACCCTAA CCCCGTCCCC TCAGGAGGAG 
    ATGCATCCCA TGCTGCAGGA CAACCCTGAT CTACATGAGC GCAGGAGACT GTTAGTGAGT 
    CCACCTCCTC CACCACGACC TCTCTCTCCT CCACACACAT ACGGCTACAT CTCCAGTCCC 
    CTCGGCCTGG ACACGGACGG CCTAGAGGAA GAGGATGATG ATGAAGAAGA GGGTGACGAA 
    ACGGACGCAG AGGTAGCTCG AGTCCACCAG CACCACATGA TGCACCCTCA CCAGCAGCAC 
    TTGCCCCAGC AGAGGGCTCT CCTGCGGGGT CTGGAACAAA CCCCGGCCTC CAGCACCTGT 
    GACCTGGAGA GCTCTGTGAC TGGGTCTATG ATTAACGGAT GGGGTTCGGC TTCAGAAGAG 
    GACAACGCCT CGTCAGGCCG TTCCAGTGCA GTCAGCTCAT CGGATGGATC GTTCTTTACC 
    GATGCCGATT TCGCCCAGGC TGTGGCAGCT GCGGCTGAAT ATGCTGGTCT TCGAGTGGCA 
    AAGCATCCTG GGAAAGGACA TCATCAGCTT CCAGTGTCTG GAGCAGCACG GAAATACCAA 
    AACCTCCCTG CGGGACACCG TCCAGGCAGC CCAGTGTCCA CAGACAGTAA CGTTAGCGCA 
    GCCCCGGGTC AGAGGAGGCC CCTGAAGAGG CAAAAACAAC ATCCAGCTGC TCAGACAGGC 
    TACCAGCGCC GAGAGGCCTA CACAGAGGAT CTCCCCCCTC CTCCGATTCC TCCTCCAGCC 
    ATGCTGAAGT CTCCAACACA CCCGGCCAAA ACCATCCCCG AGGGTCCACG GGGGGCCATG 
    TCGCCAAGAT CCAGCTCTGC GGATGGCAAG GACAGGCGAA CGGGTGCTGG CGGGCCCAGG 
    GTCAGGGACG GTTCAGACAC TCGGACCAGC TCTTCAGAGC GGAGGGATGG CCAAGATGTA 
    CAGAGGGGTC AGAGAACGGG CAAGAATAGC AAACAAGATG AAGCCGTGAA GAGCCGCCAG 
    CACCCAGGCT CAGAGGACAT CCTACCTTAC AGTCGTCCTT CTTTCCCAGC CGTCCACAGT 
    CCTCGAGGAG AACGGGACCC CAGTTCCTCC AGTTCACTGT CCTCTCGGGG GTCTGGCGGG 
    CGACGGAGAG GTGAAGGGCC ACCAGGGGCC CGCAGAAACC CTGCAGACAT TGGGCTAAAC 
    ACCATAGGAG CCTTCCAGAC TGGAGGGGAC GAGCTTGAGA TGGTGGAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359207.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214696.2 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa148916
    Clone ID Related Accession (Same CDS sequence) XM_017359207.2
    Accession Version XM_017359207.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTTGCAGA TGGCCCGATA CACTTTACTG GAGGACTCAA AGTCTGTATT ACTTCCAACA 
    GACACGGGGA CTCAGGATGG GACTAGCAAT AGGGCCACAG AGACCCCCAC TGCAGAACAC 
    GTTGACGACA ACTCTCTGGG CTACACAGGA TCCCGTCTGC GGCAGGAAGA TTACGCACCC 
    CGTATTGTTG AACACCCCTC TGATCTGATC GTATCCAAAG GCGAACCGGC CACCCTAAAC 
    TGCAAGGCTG AAGGACGTCC CACGCCCACA GTGGAGTGGT ACAAAGATGG AGAACGAGTT 
    GAAACCGACC GGGACAATCC TCGATCCCAC CGCATGCTCC TGCCCACCGG ATCTCTCTTC 
    TTTCTCCGCA TTGTTCACGG CCGGCGCAGC AAACCCGACG AAGGCTCCTA CGTTTGTGTG 
    GCACGTAACT ATCTGGGAGA AGCCGTCAGC CACAACGCCT CGCTGGAAGT AGCTATTCTC 
    CGAGATGATT TCCGACAGAA CCCTGTGGAC GTGATGGTGG CAGCGGGGGA GCCAGCTGTA 
    ATGGAGTGCC AGCCGCCCCG AGGACACCCA GAGCCCACCA TCTCCTGGAA GAAAGATGGG 
    GTCAATATAG ACGACAGAGA CGAGAGGATA ACGATACGAG GAGGAAAGCT CATGATCACC 
    AACACCAGAA AAACGGACGC GGGGAAGTAC GTGTGCGTGG GAACCAACAT GATGGGGGAA 
    CGAGAGAGCG AGATCGCGGA GTTGACTGTT CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG 
    AGCAGCCAGT CCGTCCTTGT GGATCAGAGC GTTGAGTTCA GATGTGAAGC TCAAGGAGAC 
    CCCGTGCCCA CCATCCGATG GAGGAAAGAG GACGGAGAAC TGCCCAAGGG AAGGTATGAG 
    ATCTGGGAGG ACCACACTCT CAAACTGAAG AGGCTGACTT CAGGAGACGC CGGTACTTAC 
    ACCTGTCAGG CGGAGAACAT GATGGGCAAG ACGGAGGCCA GCGCCACCCT CACCGTCCAC 
    GTTCCTCCAG TGTTTGTGGT CCGACCCAGG AATCAGGTGG CGGGTATGGG CCGCACCGTC 
    ACCTTTCAGT GTGAAGCCAC AGGAAGTCCT CAGCCTGCAA TATTCTGGCA GAAAGAAGGC 
    AGTCAGAATC TCCTGTTCTC GTCTCAGCCT CCTCCTCCGT CCAGCAGATT CTCTGTGTCT 
    CAGACTGGAG ATCTGACCAT CACAGCAGTT GAGCGTTCAG ATGGAGGATA TTATAGCTGT 
    CAGGCTCTCA ACATCGCTGG CAGTGTGATC ACCAAAGCCC TGCTGGAGGT TACAGACGTG 
    ATTTCAGACA GGCCTCCACC AATCGTCCGT CAGGGTCCAA CCAACCAGAC TGTGGCAGTG 
    GACGGGACGG TGGTTCTGAG CTGCCAAGCC ACCGGGACAC CGATTCCTAC CATCTTATGG 
    AGGAAAGATG GAGTTCTGGT GTCCACACAT GATTCCCGAC TCAAGCAGCT GGACACTGGA 
    GCCCTGCAGA TTCGATACGC AAAGCTGGGC GACACTGGCA TGTACACCTG CACGGCTTCC 
    ACCCCGAGCG GAGAGGCGTC CTGGAAGGCC TACCTGGAGG TGCAAGAGTT TGGTGCTCCA 
    GTTCAACCGG GCCGTCCCAC AGATCCCAAC CTCATCCCCA GCGCCCCATC TAAACCAGAC 
    GTCACGGATG TCAGCCGCAC CTCCGTGTCT CTTTCCTGGA AGCCGAATCT GAACGCTGGA 
    GCCACACCCA CCTCATACGT CATTGAGGCC TTCAGCCATG CAGCAGGCAG CAGCTGGCAG 
    ACGCTCGCCG ATCATGTGAA GACGGAAAGC TTCGTGCTTA AGGGTCTGAA GCCAAGTGCC 
    GTCTACCTGT TTCTGGTGAG AGCGGCCAAT GCGTACGGCC TGAGCGACCC CAGTCCCATC 
    AGCGACGCTG TCAAAACACA AGACGTCCCA CCAACCAGCC AGGGAGTGGA GCATGGCCAG 
    ATCCAGAGAG AACTGGGAGA TGTCGTCATT CACCTTCACA ATCCCACAAT TCTCTCATCG 
    TCTTCCGTCA GAGTCCAGTG GACTGTGGAG CAGCAGTCTC AGTATATTCA GGGCTATAAG 
    GTGATGTACC GTCCGTCTCC AGAAGGCGCT CCGCAGCGAG TGGATTGGGC CATGTTTGAG 
    GTCGGAGCCC CGGGGGAGCA CAGCGCTGTC GTCACCCAGC TCAAGAAGGG CATCACCTAT 
    GAGTTCAAAG TGCGCCCTTT CTTCAATGAG TTCCAGGGCA CCGACAGCGA CATCAAAGTG 
    GGAAAGACCC TGGAGGAGGC TCCCAGCGCT GCTCCCAGAG GCGTCACCGT AACAGGAAGT 
    GGAGACAACG GCACAGCAGT CCTGGTCGCC TGGCAACCGC CGCCAGAGGA GGAGCAGAAC 
    GGCGTTGTGC AGGAGTACAA GATCTGGTGT CTTGGTAATG AAAGCCGATA CCACATCAAC 
    CGCACAGTGG ATGGTTCTAC ACACTCGGTG TTGATTCCTG GTCTGGTGGC AGGAGTCACA 
    TACAGGCTAG AGGTGGCAGC AGGCACTGGG GCCGGACCCG GGGTCAAGAG CGACCCTACG 
    TCCTTCCAGC TTGACTCTTC TGGCCGTGTT CTCCCGCCGC CGCTGGACGA CGATAACACC 
    ATTTCCCAGC AGATCTCAGA CGTGGTGCGT CAGCCGGCTT TCATCGCGGG CATCGGCGCC 
    GCCTGCTGGA TCATCCTGAT GGTGTTCAGC ATCTGGCTAT ACCGCCACCG CAAGAAGAGG 
    AACGGCCTCA GCAGCAGCTA CGCCGGCATC CGCAAAGTGC CCTCCTTCAC CTTCACCCCC 
    ACAGTGGCCT ATCAGAGAGG CGGAGAGGCC GTCAGCAGTG CAGGAAGGCC GGGTTTGTTG 
    AATATAGGTG AACCAGCTAC ACAGCCCTGG CTTGCGGACA CCTGGCCCAA CAGCTGCTCC 
    AATCACAATG ACTGCACTAT TAACTGCTGC ACCGGCAGCA ATGGAAACAG CGACAGCAAC 
    CTGGCAACTT ACAGCCGCCC AGCCGACTGC ATCGCCAACT ACAGCAACCA GATGGACAAC 
    AAGGCCACCA ACCTGCTGGC TCCAGATACT GGGCTTTACA GCGACGTTGA CCTTTCAAAC 
    AAGATCAACG AGATGAAGAC CTTCAACAGC CCCAACCTGA AGGACGGGCG TTTTATGGGG 
    CCTGGTGGTC AACCCACACC CTACGCCACC ACTCAGCTCA TTCAATCCAG CCTGGCCAAC 
    AACAACATGA ACAGCAGCAG TGGCGGAAGC GGAGGAGGAA GCGCTGACTT TAATGAGAAG 
    CAGAGCTGGA AGAGCAGCCA GGGAACACCT CAGAAACAGG CCGACATCAA CACACCACTG 
    CAGTATAACG TCATGGAACA AAACAAACTC AACAAGGACC ACTACCGAGG TGAGGGTCCC 
    ATCTCAGTCA CCATCCCTTA TAACCAGGCC CAGGACGTCC TCACAGGAGA AACCTACAGC 
    AGCTCGGACA GAGGGAGCAG CACCTCTGGA AGTCAAGGGC AGAAAAAGGG TGGACGAACA 
    ACTAAACAAG CCAAACAGAA TCCAATGAAT TGGACTGAAC TGTTGCCTCC TCCTCCGGTT 
    AATCCGCCGC CATGTCAAGA ATACACCCTG GTGATGGACG ACAGTTACGA CACAGACCTG 
    CAGTGCCCTT TGCCGCCGTC TCGGATGTAT CTGCAACCTG ACGAGTTGGA AGAGGAGGAG 
    GACGAGGTGG AGCTGGAGCG TGGACCTACA CCTCCTGTAC GGGGAGCTGC TTCTTCACCT 
    GCTGCCGTGT CCTACAGCCA CCAGTCCACC GCAACCCTAA CCCCGTCCCC TCAGGAGGAG 
    ATGCATCCCA TGCTGCAGGA CAACCCTGAT CTACATGAGC GCAGGAGACT GTTAGTGAGT 
    CCACCTCCTC CACCACGACC TCTCTCTCCT CCACACACAT ACGGCTACAT CTCCAGTCCC 
    CTCGGCCTGG ACACGGACGG CCTAGAGGAA GAGGATGATG ATGAAGAAGA GGGTGACGAA 
    ACGGACGCAG AGGTAGCTCG AGTCCACCAG CACCACATGA TGCACCCTCA CCAGCAGCAC 
    TTGCCCCAGC AGAGGGCTCT CCTGCGGGGT CTGGAACAAA CCCCGGCCTC CAGCACCTGT 
    GACCTGGAGA GCTCTGTGAC TGGGTCTATG ATTAACGGAT GGGGTTCGGC TTCAGAAGAG 
    GACAACGCCT CGTCAGGCCG TTCCAGTGCA GTCAGCTCAT CGGATGGATC GTTCTTTACC 
    GATGCCGATT TCGCCCAGGC TGTGGCAGCT GCGGCTGAAT ATGCTGGTCT TCGAGTGGCA 
    AAGCATCCTG GGAAAGGACA TCATCAGCTT CCAGTGTCTG GAGCACGGAA ATACCAAAAC 
    CTCCCTGCGG GACACCGTCC AGGCAGCCCA GTGTCCACAG ACAGTAACGT TAGCGCAGCC 
    CCGGGTCAGA GGAGGCCCCT GAAGAGGCAA AAACAACATC CAGCTGCTCA GACAGGCTAC 
    CAGCGCCGAG AGGCCTACAC AGAGGATCTC CCCCCTCCTC CGATTCCTCC TCCAGCCATG 
    CTGAAGTCTC CAACACACCC GGCCAAAACC ATCCCCGAGG GTCCACGGGG GGCCATGTCG 
    CCAAGATCCA GCTCTGCGGA TGGCAAGGAC AGGCGAACGG GTGCTGGCGG GCCCAGGGTC 
    AGGGACGGTT CAGACACTCG GACCAGCTCT TCAGAGCGGA GGGATGGCCA AGATGTACAG 
    AGGGGTCAGA GAACGGGCAA GAATAGCAAA CAAGATGAAG CCGTGAAGAG CCGCCAGCAC 
    CCAGGCTCAG AGGACATCCT ACCTTACAGT CGTCCTTCTT TCCCAGCCGT CCACAGTCCT 
    CGAGGAGAAC GGGACCCCAG TTCCTCCAGT TCACTGTCCT CTCGGGGGTC TGGCGGGCGA 
    CGGAGAGGTG AAGGGCCACC AGGGGCCCGC AGAAACCCTG CAGACATTGG GCTAAACACC 
    ATAGGAGCCT TCCAGACTGG AGGGGACGAG CTTGAGATGG TGGAGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214696.2
    CDS7..5037
    Translation

    Target ORF information:

    RefSeq Version XM_017359207.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359207.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTTGCAGA TGGCCCGATA CACTTTACTG GAGGACTCAA AGTCTGTATT ACTTCCAACA 
    GACACGGGGA CTCAGGATGG GACTAGCAAT AGGGCCACAG AGACCCCCAC TGCAGAACAC 
    GTTGACGACA ACTCTCTGGG CTACACAGGA TCCCGTCTGC GGCAGGAAGA TTACGCACCC 
    CGTATTGTTG AACACCCCTC TGATCTGATC GTATCCAAAG GCGAACCGGC CACCCTAAAC 
    TGCAAGGCTG AAGGACGTCC CACGCCCACA GTGGAGTGGT ACAAAGATGG AGAACGAGTT 
    GAAACCGACC GGGACAATCC TCGATCCCAC CGCATGCTCC TGCCCACCGG ATCTCTCTTC 
    TTTCTCCGCA TTGTTCACGG CCGGCGCAGC AAACCCGACG AAGGCTCCTA CGTTTGTGTG 
    GCACGTAACT ATCTGGGAGA AGCCGTCAGC CACAACGCCT CGCTGGAAGT AGCTATTCTC 
    CGAGATGATT TCCGACAGAA CCCTGTGGAC GTGATGGTGG CAGCGGGGGA GCCAGCTGTA 
    ATGGAGTGCC AGCCGCCCCG AGGACACCCA GAGCCCACCA TCTCCTGGAA GAAAGATGGG 
    GTCAATATAG ACGACAGAGA CGAGAGGATA ACGATACGAG GAGGAAAGCT CATGATCACC 
    AACACCAGAA AAACGGACGC GGGGAAGTAC GTGTGCGTGG GAACCAACAT GATGGGGGAA 
    CGAGAGAGCG AGATCGCGGA GTTGACTGTT CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG 
    AGCAGCCAGT CCGTCCTTGT GGATCAGAGC GTTGAGTTCA GATGTGAAGC TCAAGGAGAC 
    CCCGTGCCCA CCATCCGATG GAGGAAAGAG GACGGAGAAC TGCCCAAGGG AAGGTATGAG 
    ATCTGGGAGG ACCACACTCT CAAACTGAAG AGGCTGACTT CAGGAGACGC CGGTACTTAC 
    ACCTGTCAGG CGGAGAACAT GATGGGCAAG ACGGAGGCCA GCGCCACCCT CACCGTCCAC 
    GTTCCTCCAG TGTTTGTGGT CCGACCCAGG AATCAGGTGG CGGGTATGGG CCGCACCGTC 
    ACCTTTCAGT GTGAAGCCAC AGGAAGTCCT CAGCCTGCAA TATTCTGGCA GAAAGAAGGC 
    AGTCAGAATC TCCTGTTCTC GTCTCAGCCT CCTCCTCCGT CCAGCAGATT CTCTGTGTCT 
    CAGACTGGAG ATCTGACCAT CACAGCAGTT GAGCGTTCAG ATGGAGGATA TTATAGCTGT 
    CAGGCTCTCA ACATCGCTGG CAGTGTGATC ACCAAAGCCC TGCTGGAGGT TACAGACGTG 
    ATTTCAGACA GGCCTCCACC AATCGTCCGT CAGGGTCCAA CCAACCAGAC TGTGGCAGTG 
    GACGGGACGG TGGTTCTGAG CTGCCAAGCC ACCGGGACAC CGATTCCTAC CATCTTATGG 
    AGGAAAGATG GAGTTCTGGT GTCCACACAT GATTCCCGAC TCAAGCAGCT GGACACTGGA 
    GCCCTGCAGA TTCGATACGC AAAGCTGGGC GACACTGGCA TGTACACCTG CACGGCTTCC 
    ACCCCGAGCG GAGAGGCGTC CTGGAAGGCC TACCTGGAGG TGCAAGAGTT TGGTGCTCCA 
    GTTCAACCGG GCCGTCCCAC AGATCCCAAC CTCATCCCCA GCGCCCCATC TAAACCAGAC 
    GTCACGGATG TCAGCCGCAC CTCCGTGTCT CTTTCCTGGA AGCCGAATCT GAACGCTGGA 
    GCCACACCCA CCTCATACGT CATTGAGGCC TTCAGCCATG CAGCAGGCAG CAGCTGGCAG 
    ACGCTCGCCG ATCATGTGAA GACGGAAAGC TTCGTGCTTA AGGGTCTGAA GCCAAGTGCC 
    GTCTACCTGT TTCTGGTGAG AGCGGCCAAT GCGTACGGCC TGAGCGACCC CAGTCCCATC 
    AGCGACGCTG TCAAAACACA AGACGTCCCA CCAACCAGCC AGGGAGTGGA GCATGGCCAG 
    ATCCAGAGAG AACTGGGAGA TGTCGTCATT CACCTTCACA ATCCCACAAT TCTCTCATCG 
    TCTTCCGTCA GAGTCCAGTG GACTGTGGAG CAGCAGTCTC AGTATATTCA GGGCTATAAG 
    GTGATGTACC GTCCGTCTCC AGAAGGCGCT CCGCAGCGAG TGGATTGGGC CATGTTTGAG 
    GTCGGAGCCC CGGGGGAGCA CAGCGCTGTC GTCACCCAGC TCAAGAAGGG CATCACCTAT 
    GAGTTCAAAG TGCGCCCTTT CTTCAATGAG TTCCAGGGCA CCGACAGCGA CATCAAAGTG 
    GGAAAGACCC TGGAGGAGGC TCCCAGCGCT GCTCCCAGAG GCGTCACCGT AACAGGAAGT 
    GGAGACAACG GCACAGCAGT CCTGGTCGCC TGGCAACCGC CGCCAGAGGA GGAGCAGAAC 
    GGCGTTGTGC AGGAGTACAA GATCTGGTGT CTTGGTAATG AAAGCCGATA CCACATCAAC 
    CGCACAGTGG ATGGTTCTAC ACACTCGGTG TTGATTCCTG GTCTGGTGGC AGGAGTCACA 
    TACAGGCTAG AGGTGGCAGC AGGCACTGGG GCCGGACCCG GGGTCAAGAG CGACCCTACG 
    TCCTTCCAGC TTGACTCTTC TGGCCGTGTT CTCCCGCCGC CGCTGGACGA CGATAACACC 
    ATTTCCCAGC AGATCTCAGA CGTGGTGCGT CAGCCGGCTT TCATCGCGGG CATCGGCGCC 
    GCCTGCTGGA TCATCCTGAT GGTGTTCAGC ATCTGGCTAT ACCGCCACCG CAAGAAGAGG 
    AACGGCCTCA GCAGCAGCTA CGCCGGCATC CGCAAAGTGC CCTCCTTCAC CTTCACCCCC 
    ACAGTGGCCT ATCAGAGAGG CGGAGAGGCC GTCAGCAGTG CAGGAAGGCC GGGTTTGTTG 
    AATATAGGTG AACCAGCTAC ACAGCCCTGG CTTGCGGACA CCTGGCCCAA CAGCTGCTCC 
    AATCACAATG ACTGCACTAT TAACTGCTGC ACCGGCAGCA ATGGAAACAG CGACAGCAAC 
    CTGGCAACTT ACAGCCGCCC AGCCGACTGC ATCGCCAACT ACAGCAACCA GATGGACAAC 
    AAGGCCACCA ACCTGCTGGC TCCAGATACT GGGCTTTACA GCGACGTTGA CCTTTCAAAC 
    AAGATCAACG AGATGAAGAC CTTCAACAGC CCCAACCTGA AGGACGGGCG TTTTATGGGG 
    CCTGGTGGTC AACCCACACC CTACGCCACC ACTCAGCTCA TTCAATCCAG CCTGGCCAAC 
    AACAACATGA ACAGCAGCAG TGGCGGAAGC GGAGGAGGAA GCGCTGACTT TAATGAGAAG 
    CAGAGCTGGA AGAGCAGCCA GGGAACACCT CAGAAACAGG CCGACATCAA CACACCACTG 
    CAGTATAACG TCATGGAACA AAACAAACTC AACAAGGACC ACTACCGAGG TGAGGGTCCC 
    ATCTCAGTCA CCATCCCTTA TAACCAGGCC CAGGACGTCC TCACAGGAGA AACCTACAGC 
    AGCTCGGACA GAGGGAGCAG CACCTCTGGA AGTCAAGGGC AGAAAAAGGG TGGACGAACA 
    ACTAAACAAG CCAAACAGAA TCCAATGAAT TGGACTGAAC TGTTGCCTCC TCCTCCGGTT 
    AATCCGCCGC CATGTCAAGA ATACACCCTG GTGATGGACG ACAGTTACGA CACAGACCTG 
    CAGTGCCCTT TGCCGCCGTC TCGGATGTAT CTGCAACCTG ACGAGTTGGA AGAGGAGGAG 
    GACGAGGTGG AGCTGGAGCG TGGACCTACA CCTCCTGTAC GGGGAGCTGC TTCTTCACCT 
    GCTGCCGTGT CCTACAGCCA CCAGTCCACC GCAACCCTAA CCCCGTCCCC TCAGGAGGAG 
    ATGCATCCCA TGCTGCAGGA CAACCCTGAT CTACATGAGC GCAGGAGACT GTTAGTGAGT 
    CCACCTCCTC CACCACGACC TCTCTCTCCT CCACACACAT ACGGCTACAT CTCCAGTCCC 
    CTCGGCCTGG ACACGGACGG CCTAGAGGAA GAGGATGATG ATGAAGAAGA GGGTGACGAA 
    ACGGACGCAG AGGTAGCTCG AGTCCACCAG CACCACATGA TGCACCCTCA CCAGCAGCAC 
    TTGCCCCAGC AGAGGGCTCT CCTGCGGGGT CTGGAACAAA CCCCGGCCTC CAGCACCTGT 
    GACCTGGAGA GCTCTGTGAC TGGGTCTATG ATTAACGGAT GGGGTTCGGC TTCAGAAGAG 
    GACAACGCCT CGTCAGGCCG TTCCAGTGCA GTCAGCTCAT CGGATGGATC GTTCTTTACC 
    GATGCCGATT TCGCCCAGGC TGTGGCAGCT GCGGCTGAAT ATGCTGGTCT TCGAGTGGCA 
    AAGCATCCTG GGAAAGGACA TCATCAGCTT CCAGTGTCTG GAGCACGGAA ATACCAAAAC 
    CTCCCTGCGG GACACCGTCC AGGCAGCCCA GTGTCCACAG ACAGTAACGT TAGCGCAGCC 
    CCGGGTCAGA GGAGGCCCCT GAAGAGGCAA AAACAACATC CAGCTGCTCA GACAGGCTAC 
    CAGCGCCGAG AGGCCTACAC AGAGGATCTC CCCCCTCCTC CGATTCCTCC TCCAGCCATG 
    CTGAAGTCTC CAACACACCC GGCCAAAACC ATCCCCGAGG GTCCACGGGG GGCCATGTCG 
    CCAAGATCCA GCTCTGCGGA TGGCAAGGAC AGGCGAACGG GTGCTGGCGG GCCCAGGGTC 
    AGGGACGGTT CAGACACTCG GACCAGCTCT TCAGAGCGGA GGGATGGCCA AGATGTACAG 
    AGGGGTCAGA GAACGGGCAA GAATAGCAAA CAAGATGAAG CCGTGAAGAG CCGCCAGCAC 
    CCAGGCTCAG AGGACATCCT ACCTTACAGT CGTCCTTCTT TCCCAGCCGT CCACAGTCCT 
    CGAGGAGAAC GGGACCCCAG TTCCTCCAGT TCACTGTCCT CTCGGGGGTC TGGCGGGCGA 
    CGGAGAGGTG AAGGGCCACC AGGGGCCCGC AGAAACCCTG CAGACATTGG GCTAAACACC 
    ATAGGAGCCT TCCAGACTGG AGGGGACGAG CTTGAGATGG TGGAGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359212.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214701.2 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa148917
    Clone ID Related Accession (Same CDS sequence) XM_017359212.2
    Accession Version XM_017359212.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359212.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGTTTA TCCCTCTGGT GGTTTCCATT TTGGCAATGC TAGTCATGAT GGGTGTCCAA 
    CATGGAGGAA CTCCAGGAGT CCCTCGCCTT GCTAGCCTCA GGAACCAACC AAACCAATGG 
    TGTAAGCGTC CACACGGGAG CTTTCTAGTC GGAGCCCATC ACCGCCCCAC GCAACAGCGC 
    CATCACAACA GCAAGAAGCA TAAATGCTTC CAGGACTTCC AGAGATCCCG TCTGCGGCAG 
    GAAGATTACG CACCCCGTAT TGTTGAACAC CCCTCTGATC TGATCGTATC CAAAGGCGAA 
    CCGGCCACCC TAAACTGCAA GGCTGAAGGA CGTCCCACGC CCACAGTGGA GTGGTACAAA 
    GATGGAGAAC GAGTTGAAAC CGACCGGGAC AATCCTCGAT CCCACCGCAT GCTCCTGCCC 
    ACCGGATCTC TCTTCTTTCT CCGCATTGTT CACGGCCGGC GCAGCAAACC CGACGAAGGC 
    TCCTACGTTT GTGTGGCACG TAACTATCTG GGAGAAGCCG TCAGCCACAA CGCCTCGCTG 
    GAAGTAGCTA TTCTCCGAGA TGATTTCCGA CAGAACCCTG TGGACGTGAT GGTGGCAGCG 
    GGGGAGCCAG CTGTAATGGA GTGCCAGCCG CCCCGAGGAC ACCCAGAGCC CACCATCTCC 
    TGGAAGAAAG ATGGGGTCAA TATAGACGAC AGAGACGAGA GGATAACGAT ACGAGGAGGA 
    AAGCTCATGA TCACCAACAC CAGAAAAACG GACGCGGGGA AGTACGTGTG CGTGGGAACC 
    AACATGATGG GGGAACGAGA GAGCGAGATC GCGGAGTTGA CTGTTCTCGA GAGGCCCAGT 
    TTCTTGCGAA AGCCGAGCAG CCAGTCCGTC CTTGTGGATC AGAGCGTTGA GTTCAGATGT 
    GAAGCTCAAG GAGACCCCGT GCCCACCATC CGATGGAGGA AAGAGGACGG AGAACTGCCC 
    AAGGGAAGGT ATGAGATCTG GGAGGACCAC ACTCTCAAAC TGAAGAGGCT GACTTCAGGA 
    GACGCCGGTA CTTACACCTG TCAGGCGGAG AACATGATGG GCAAGACGGA GGCCAGCGCC 
    ACCCTCACCG TCCACGTTGT GTCTGTTCCT CCAGTGTTTG TGGTCCGACC CAGGAATCAG 
    GTGGCGGGTA TGGGCCGCAC CGTCACCTTT CAGTGTGAAG CCACAGGAAG TCCTCAGCCT 
    GCAATATTCT GGCAGAAAGA AGGCAGTCAG AATCTCCTGT TCTCGTCTCA GCCTCCTCCT 
    CCGTCCAGCA GATTCTCTGT GTCTCAGACT GGAGATCTGA CCATCACAGC AGTTGAGCGT 
    TCAGATGGAG GATATTATAG CTGTCAGGCT CTCAACATCG CTGGCAGTGT GATCACCAAA 
    GCCCTGCTGG AGGTTACAGA CGTGATTTCA GACAGGCCTC CACCAATCGT CCGTCAGGGT 
    CCAACCAACC AGACTGTGGC AGTGGACGGG ACGGTGGTTC TGAGCTGCCA AGCCACCGGG 
    ACACCGATTC CTACCATCTT ATGGAGGAAA GATGGAGTTC TGGTGTCCAC ACATGATTCC 
    CGACTCAAGC AGCTGGACAC TGGAGCCCTG CAGATTCGAT ACGCAAAGCT GGGCGACACT 
    GGCATGTACA CCTGCACGGC TTCCACCCCG AGCGGAGAGG CGTCCTGGAA GGCCTACCTG 
    GAGGTGCAAG AGTTTGGTGC TCCAGTTCAA CCGGGCCGTC CCACAGATCC CAACCTCATC 
    CCCAGCGCCC CATCTAAACC AGACGTCACG GATGTCAGCC GCACCTCCGT GTCTCTTTCC 
    TGGAAGCCGA ATCTGAACGC TGGAGCCACA CCCACCTCAT ACGTCATTGA GGCCTTCAGC 
    CATGCAGCAG GCAGCAGCTG GCAGACGCTC GCCGATCATG TGAAGACGGA AAGCTTCGTG 
    CTTAAGGGTC TGAAGCCAAG TGCCGTCTAC CTGTTTCTGG TGAGAGCGGC CAATGCGTAC 
    GGCCTGAGCG ACCCCAGTCC CATCAGCGAC GCTGTCAAAA CACAAGACGT CCCACCAACC 
    AGCCAGGGAG TGGAGCATGG CCAGATCCAG AGAGAACTGG GAGATGTCGT CATTCACCTT 
    CACAATCCCA CAATTCTCTC ATCGTCTTCC GTCAGAGTCC AGTGGACTGT GGAGCAGCAG 
    TCTCAGTATA TTCAGGGCTA TAAGGTGATG TACCGTCCGT CTCCAGAAGG CGCTCCGCAG 
    CGAGTGGATT GGGCCATGTT TGAGGTCGGA GCCCCGGGGG AGCACAGCGC TGTCGTCACC 
    CAGCTCAAGA AGGGCATCAC CTATGAGTTC AAAGTGCGCC CTTTCTTCAA TGAGTTCCAG 
    GGCACCGACA GCGACATCAA AGTGGGAAAG ACCCTGGAGG AGGCTCCCAG CGCTGCTCCC 
    AGAGGCGTCA CCGTAACAGG AAGTGGAGAC AACGGCACAG CAGTCCTGGT CGCCTGGCAA 
    CCGCCGCCAG AGGAGGAGCA GAACGGCGTT GTGCAGGAGT ACAAGATCTG GTGTCTTGGT 
    AATGAAAGCC GATACCACAT CAACCGCACA GTGGATGGTT CTACACACTC GGTGTTGATT 
    CCTGGTCTGG TGGCAGGAGT CACATACAGG CTAGAGGTGG CAGCAGGCAC TGGGGCCGGA 
    CCCGGGGTCA AGAGCGACCC TACGTCCTTC CAGCTTGACT CTTCTGGCCG TGTTCTCCCG 
    CCGCCGCTGG ACGACGATAA CACCATTTCC CAGCAGATCT CAGACGTGGT GCGTCAGCCG 
    GCTTTCATCG CGGGCATCGG CGCCGCCTGC TGGATCATCC TGATGGTGTT CAGCATCTGG 
    CTATACCGCC ACCGCAAGAA GAGGAACGGC CTCAGCAGCA GCTACGCCGG CATCCGCAAA 
    GTGCCCTCCT TCACCTTCAC CCCCACAGTG GCCTATCAGA GAGGCGGAGA GGCCGTCAGC 
    AGTGCAGGAA GGCCGGGTTT GTTGAATATA GGTGAACCAG CTACACAGCC CTGGCTTGCG 
    GACACCTGGC CCAACAGCTG CTCCAATCAC AATGACTGCA CTATTAACTG CTGCACCGGC 
    AGCAATGGAA ACAGCGACAG CAACCTGGCA ACTTACAGCC GCCCAGCCGA CTGCATCGCC 
    AACTACAGCA ACCAGATGGA CAACAAGGCC ACCAACCTGC TGGCTCCAGA TACTGGGCTT 
    TACAGCGACG TTGACCTTTC AAACAAGATC AACGAGATGA AGACCTTCAA CAGCCCCAAC 
    CTGAAGGACG GGCGTTTTAT GGGGCCTGGT GGTCAACCCA CACCCTACGC CACCACTCAG 
    CTCATTCAAT CCAGCCTGGC CAACAACAAC ATGAACAGCA GCAGTGGCGG AAGCGGAGGA 
    GGAAGCGCTG ACTTTAATGA GAAGCAGAGC TGGAAGAGCA GCCAGGGAAC ACCTCAGAAA 
    CAGGCCGACA TCAACACACC ACTGCAGTAT AACGTCATGG AACAAAACAA ACTCAACAAG 
    GACCACTACC GAGGTGAGGG TCCCATCTCA GTCACCATCC CTTATAACCA GGCCCAGGAC 
    GTCCTCACAG GAGAAACCTA CAGCAGCTCG GACAGAGGGA GCAGCACCTC TGGAAGTCAA 
    GGGCAGAAAA AGGGTGGACG AACAACTAAA CAAGCCAAAC AGAATCCAAT GAATTGGACT 
    GAACTGTTGC CTCCTCCTCC GGTTAATCCG CCGCCATGTC AAGAATACAC CCTGGTGATG 
    GACGACAGTT ACGACACAGA CCTGCAGTGC CCTTTGCCGC CGTCTCGGAT GTATCTGCAA 
    CCTGACGAGT TGGAAGAGGA GGAGGACGAG GTGGAGCTGG AGCGTGGACC TACACCTCCT 
    GTACGGGGAG CTGCTTCTTC ACCTGCTGCC GTGTCCTACA GCCACCAGTC CACCGCAACC 
    CTAACCCCGT CCCCTCAGGA GGAGATGCAT CCCATGCTGC AGGACAACCC TGATCTACAT 
    GAGCGCAGGA GACTGTTAGT GAGTCCACCT CCTCCACCAC GACCTCTCTC TCCTCCACAC 
    ACATACGGCT ACATCTCCAG TCCCCTCGGC CTGGACACGG ACGGCCTAGA GGAAGAGGAT 
    GATGATGAAG AAGAGGGTGA CGAAACGGAC GCAGAGGTAG CTCGAGTCCA CCAGCACCAC 
    ATGATGCACC CTCACCAGCA GCACTTGCCC CAGCAGAGGG CTCTCCTGCG GGGTCTGGAA 
    CAAACCCCGG CCTCCAGCAC CTGTGACCTG GAGAGCTCTG TGACTGGGTC TATGATTAAC 
    GGATGGGGTT CGGCTTCAGA AGAGGACAAC GCCTCGTCAG GCCGTTCCAG TGCAGTCAGC 
    TCATCGGATG GATCGTTCTT TACCGATGCC GATTTCGCCC AGGCTGTGGC AGCTGCGGCT 
    GAATATGCTG GTCTTCGAGT GGCAAAGCAT CCTGGGAAAG GACATCATCA GCTTCCAGTG 
    TCTGGAGCAG CACGGAAATA CCAAAACCTC CCTGCGGGAC ACCGTCCAGG CAGCCCAGTG 
    TCCACAGACA GTAACGTTAG CGCAGCCCCG GGTCAGAGGA GGCCCCTGAA GAGGCAAAAA 
    CAACATCCAG CTGCTCAGAC AGGCTACCAG CGCCGAGAGG CCTACACAGA GGATCTCCCC 
    CCTCCTCCGA TTCCTCCTCC AGCCATGCTG AAGTCTCCAA CACACCCGGC CAAAACCATC 
    CCCGAGGGTC CACGGGGGGC CATGTCGCCA AGATCCAGCT CTGCGGATGG CAAGGACAGG 
    CGAACGGGTG CTGGCGGGCC CAGGGTCAGG GACGGTTCAG ACACTCGGAC CAGCTCTTCA 
    GAGCGGAGGG ATGGCCAAGA TGTACAGAGG GGTCAGAGAA CGGGCAAGAA TAGCAAACAA 
    GATGAAGCCG TGAAGAGCCG CCAGCACCCA GGCTCAGAGG ACATCCTACC TTACAGTCGT 
    CCTTCTTTCC CAGCCGTCCA CAGTCCTCGA GGAGAACGGG ACCCCAGTTC CTCCAGTTCA 
    CTGTCCTCTC GGGGGTCTGG CGGGCGACGG AGAGGTGAAG GGCCACCAGG GGCCCGCAGA 
    AACCCTGCAG ACATTGGGCT AAACACCATA GGAGCCTTCC AGACTGGAGG GGACGAGCTT 
    GAGATGGTGG AGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214701.2
    CDS16..5133
    Translation

    Target ORF information:

    RefSeq Version XM_017359212.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359212.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGTTTA TCCCTCTGGT GGTTTCCATT TTGGCAATGC TAGTCATGAT GGGTGTCCAA 
    CATGGAGGAA CTCCAGGAGT CCCTCGCCTT GCTAGCCTCA GGAACCAACC AAACCAATGG 
    TGTAAGCGTC CACACGGGAG CTTTCTAGTC GGAGCCCATC ACCGCCCCAC GCAACAGCGC 
    CATCACAACA GCAAGAAGCA TAAATGCTTC CAGGACTTCC AGAGATCCCG TCTGCGGCAG 
    GAAGATTACG CACCCCGTAT TGTTGAACAC CCCTCTGATC TGATCGTATC CAAAGGCGAA 
    CCGGCCACCC TAAACTGCAA GGCTGAAGGA CGTCCCACGC CCACAGTGGA GTGGTACAAA 
    GATGGAGAAC GAGTTGAAAC CGACCGGGAC AATCCTCGAT CCCACCGCAT GCTCCTGCCC 
    ACCGGATCTC TCTTCTTTCT CCGCATTGTT CACGGCCGGC GCAGCAAACC CGACGAAGGC 
    TCCTACGTTT GTGTGGCACG TAACTATCTG GGAGAAGCCG TCAGCCACAA CGCCTCGCTG 
    GAAGTAGCTA TTCTCCGAGA TGATTTCCGA CAGAACCCTG TGGACGTGAT GGTGGCAGCG 
    GGGGAGCCAG CTGTAATGGA GTGCCAGCCG CCCCGAGGAC ACCCAGAGCC CACCATCTCC 
    TGGAAGAAAG ATGGGGTCAA TATAGACGAC AGAGACGAGA GGATAACGAT ACGAGGAGGA 
    AAGCTCATGA TCACCAACAC CAGAAAAACG GACGCGGGGA AGTACGTGTG CGTGGGAACC 
    AACATGATGG GGGAACGAGA GAGCGAGATC GCGGAGTTGA CTGTTCTCGA GAGGCCCAGT 
    TTCTTGCGAA AGCCGAGCAG CCAGTCCGTC CTTGTGGATC AGAGCGTTGA GTTCAGATGT 
    GAAGCTCAAG GAGACCCCGT GCCCACCATC CGATGGAGGA AAGAGGACGG AGAACTGCCC 
    AAGGGAAGGT ATGAGATCTG GGAGGACCAC ACTCTCAAAC TGAAGAGGCT GACTTCAGGA 
    GACGCCGGTA CTTACACCTG TCAGGCGGAG AACATGATGG GCAAGACGGA GGCCAGCGCC 
    ACCCTCACCG TCCACGTTGT GTCTGTTCCT CCAGTGTTTG TGGTCCGACC CAGGAATCAG 
    GTGGCGGGTA TGGGCCGCAC CGTCACCTTT CAGTGTGAAG CCACAGGAAG TCCTCAGCCT 
    GCAATATTCT GGCAGAAAGA AGGCAGTCAG AATCTCCTGT TCTCGTCTCA GCCTCCTCCT 
    CCGTCCAGCA GATTCTCTGT GTCTCAGACT GGAGATCTGA CCATCACAGC AGTTGAGCGT 
    TCAGATGGAG GATATTATAG CTGTCAGGCT CTCAACATCG CTGGCAGTGT GATCACCAAA 
    GCCCTGCTGG AGGTTACAGA CGTGATTTCA GACAGGCCTC CACCAATCGT CCGTCAGGGT 
    CCAACCAACC AGACTGTGGC AGTGGACGGG ACGGTGGTTC TGAGCTGCCA AGCCACCGGG 
    ACACCGATTC CTACCATCTT ATGGAGGAAA GATGGAGTTC TGGTGTCCAC ACATGATTCC 
    CGACTCAAGC AGCTGGACAC TGGAGCCCTG CAGATTCGAT ACGCAAAGCT GGGCGACACT 
    GGCATGTACA CCTGCACGGC TTCCACCCCG AGCGGAGAGG CGTCCTGGAA GGCCTACCTG 
    GAGGTGCAAG AGTTTGGTGC TCCAGTTCAA CCGGGCCGTC CCACAGATCC CAACCTCATC 
    CCCAGCGCCC CATCTAAACC AGACGTCACG GATGTCAGCC GCACCTCCGT GTCTCTTTCC 
    TGGAAGCCGA ATCTGAACGC TGGAGCCACA CCCACCTCAT ACGTCATTGA GGCCTTCAGC 
    CATGCAGCAG GCAGCAGCTG GCAGACGCTC GCCGATCATG TGAAGACGGA AAGCTTCGTG 
    CTTAAGGGTC TGAAGCCAAG TGCCGTCTAC CTGTTTCTGG TGAGAGCGGC CAATGCGTAC 
    GGCCTGAGCG ACCCCAGTCC CATCAGCGAC GCTGTCAAAA CACAAGACGT CCCACCAACC 
    AGCCAGGGAG TGGAGCATGG CCAGATCCAG AGAGAACTGG GAGATGTCGT CATTCACCTT 
    CACAATCCCA CAATTCTCTC ATCGTCTTCC GTCAGAGTCC AGTGGACTGT GGAGCAGCAG 
    TCTCAGTATA TTCAGGGCTA TAAGGTGATG TACCGTCCGT CTCCAGAAGG CGCTCCGCAG 
    CGAGTGGATT GGGCCATGTT TGAGGTCGGA GCCCCGGGGG AGCACAGCGC TGTCGTCACC 
    CAGCTCAAGA AGGGCATCAC CTATGAGTTC AAAGTGCGCC CTTTCTTCAA TGAGTTCCAG 
    GGCACCGACA GCGACATCAA AGTGGGAAAG ACCCTGGAGG AGGCTCCCAG CGCTGCTCCC 
    AGAGGCGTCA CCGTAACAGG AAGTGGAGAC AACGGCACAG CAGTCCTGGT CGCCTGGCAA 
    CCGCCGCCAG AGGAGGAGCA GAACGGCGTT GTGCAGGAGT ACAAGATCTG GTGTCTTGGT 
    AATGAAAGCC GATACCACAT CAACCGCACA GTGGATGGTT CTACACACTC GGTGTTGATT 
    CCTGGTCTGG TGGCAGGAGT CACATACAGG CTAGAGGTGG CAGCAGGCAC TGGGGCCGGA 
    CCCGGGGTCA AGAGCGACCC TACGTCCTTC CAGCTTGACT CTTCTGGCCG TGTTCTCCCG 
    CCGCCGCTGG ACGACGATAA CACCATTTCC CAGCAGATCT CAGACGTGGT GCGTCAGCCG 
    GCTTTCATCG CGGGCATCGG CGCCGCCTGC TGGATCATCC TGATGGTGTT CAGCATCTGG 
    CTATACCGCC ACCGCAAGAA GAGGAACGGC CTCAGCAGCA GCTACGCCGG CATCCGCAAA 
    GTGCCCTCCT TCACCTTCAC CCCCACAGTG GCCTATCAGA GAGGCGGAGA GGCCGTCAGC 
    AGTGCAGGAA GGCCGGGTTT GTTGAATATA GGTGAACCAG CTACACAGCC CTGGCTTGCG 
    GACACCTGGC CCAACAGCTG CTCCAATCAC AATGACTGCA CTATTAACTG CTGCACCGGC 
    AGCAATGGAA ACAGCGACAG CAACCTGGCA ACTTACAGCC GCCCAGCCGA CTGCATCGCC 
    AACTACAGCA ACCAGATGGA CAACAAGGCC ACCAACCTGC TGGCTCCAGA TACTGGGCTT 
    TACAGCGACG TTGACCTTTC AAACAAGATC AACGAGATGA AGACCTTCAA CAGCCCCAAC 
    CTGAAGGACG GGCGTTTTAT GGGGCCTGGT GGTCAACCCA CACCCTACGC CACCACTCAG 
    CTCATTCAAT CCAGCCTGGC CAACAACAAC ATGAACAGCA GCAGTGGCGG AAGCGGAGGA 
    GGAAGCGCTG ACTTTAATGA GAAGCAGAGC TGGAAGAGCA GCCAGGGAAC ACCTCAGAAA 
    CAGGCCGACA TCAACACACC ACTGCAGTAT AACGTCATGG AACAAAACAA ACTCAACAAG 
    GACCACTACC GAGGTGAGGG TCCCATCTCA GTCACCATCC CTTATAACCA GGCCCAGGAC 
    GTCCTCACAG GAGAAACCTA CAGCAGCTCG GACAGAGGGA GCAGCACCTC TGGAAGTCAA 
    GGGCAGAAAA AGGGTGGACG AACAACTAAA CAAGCCAAAC AGAATCCAAT GAATTGGACT 
    GAACTGTTGC CTCCTCCTCC GGTTAATCCG CCGCCATGTC AAGAATACAC CCTGGTGATG 
    GACGACAGTT ACGACACAGA CCTGCAGTGC CCTTTGCCGC CGTCTCGGAT GTATCTGCAA 
    CCTGACGAGT TGGAAGAGGA GGAGGACGAG GTGGAGCTGG AGCGTGGACC TACACCTCCT 
    GTACGGGGAG CTGCTTCTTC ACCTGCTGCC GTGTCCTACA GCCACCAGTC CACCGCAACC 
    CTAACCCCGT CCCCTCAGGA GGAGATGCAT CCCATGCTGC AGGACAACCC TGATCTACAT 
    GAGCGCAGGA GACTGTTAGT GAGTCCACCT CCTCCACCAC GACCTCTCTC TCCTCCACAC 
    ACATACGGCT ACATCTCCAG TCCCCTCGGC CTGGACACGG ACGGCCTAGA GGAAGAGGAT 
    GATGATGAAG AAGAGGGTGA CGAAACGGAC GCAGAGGTAG CTCGAGTCCA CCAGCACCAC 
    ATGATGCACC CTCACCAGCA GCACTTGCCC CAGCAGAGGG CTCTCCTGCG GGGTCTGGAA 
    CAAACCCCGG CCTCCAGCAC CTGTGACCTG GAGAGCTCTG TGACTGGGTC TATGATTAAC 
    GGATGGGGTT CGGCTTCAGA AGAGGACAAC GCCTCGTCAG GCCGTTCCAG TGCAGTCAGC 
    TCATCGGATG GATCGTTCTT TACCGATGCC GATTTCGCCC AGGCTGTGGC AGCTGCGGCT 
    GAATATGCTG GTCTTCGAGT GGCAAAGCAT CCTGGGAAAG GACATCATCA GCTTCCAGTG 
    TCTGGAGCAG CACGGAAATA CCAAAACCTC CCTGCGGGAC ACCGTCCAGG CAGCCCAGTG 
    TCCACAGACA GTAACGTTAG CGCAGCCCCG GGTCAGAGGA GGCCCCTGAA GAGGCAAAAA 
    CAACATCCAG CTGCTCAGAC AGGCTACCAG CGCCGAGAGG CCTACACAGA GGATCTCCCC 
    CCTCCTCCGA TTCCTCCTCC AGCCATGCTG AAGTCTCCAA CACACCCGGC CAAAACCATC 
    CCCGAGGGTC CACGGGGGGC CATGTCGCCA AGATCCAGCT CTGCGGATGG CAAGGACAGG 
    CGAACGGGTG CTGGCGGGCC CAGGGTCAGG GACGGTTCAG ACACTCGGAC CAGCTCTTCA 
    GAGCGGAGGG ATGGCCAAGA TGTACAGAGG GGTCAGAGAA CGGGCAAGAA TAGCAAACAA 
    GATGAAGCCG TGAAGAGCCG CCAGCACCCA GGCTCAGAGG ACATCCTACC TTACAGTCGT 
    CCTTCTTTCC CAGCCGTCCA CAGTCCTCGA GGAGAACGGG ACCCCAGTTC CTCCAGTTCA 
    CTGTCCTCTC GGGGGTCTGG CGGGCGACGG AGAGGTGAAG GGCCACCAGG GGCCCGCAGA 
    AACCCTGCAG ACATTGGGCT AAACACCATA GGAGCCTTCC AGACTGGAGG GGACGAGCTT 
    GAGATGGTGG AGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_021481331.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337006.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa148918
    Clone ID Related Accession (Same CDS sequence) XM_021481331.1
    Accession Version XM_021481331.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4980bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGGGCAG GGCTGCTGTT TCTCATCTTC CTCCTCCTGG ATCTTGGGAT CTGCTGGGAT 
    GGAGACAAGC CTGGGAGAAA AAAGGGATCC CGTCTGCGGC AGGAAGATTA CGCACCCCGT 
    ATTGTTGAAC ACCCCTCTGA TCTGATCGTA TCCAAAGGCG AACCGGCCAC CCTAAACTGC 
    AAGGCTGAAG GACGTCCCAC GCCCACAGTG GAGTGGTACA AAGATGGAGA ACGAGTTGAA 
    ACCGACCGGG ACAATCCTCG ATCCCACCGC ATGCTCCTGC CCACCGGATC TCTCTTCTTT 
    CTCCGCATTG TTCACGGCCG GCGCAGCAAA CCCGACGAAG GCTCCTACGT TTGTGTGGCA 
    CGTAACTATC TGGGAGAAGC CGTCAGCCAC AACGCCTCGC TGGAAGTAGC TATTCTCCGA 
    GATGATTTCC GACAGAACCC TGTGGACGTG ATGGTGGCAG CGGGGGAGCC AGCTGTAATG 
    GAGTGCCAGC CGCCCCGAGG ACACCCAGAG CCCACCATCT CCTGGAAGAA AGATGGGGTC 
    AATATAGACG ACAGAGACGA GAGGATAACG ATACGAGGAG GAAAGCTCAT GATCACCAAC 
    ACCAGAAAAA CGGACGCGGG GAAGTACGTG TGCGTGGGAA CCAACATGAT GGGGGAACGA 
    GAGAGCGAGA TCGCGGAGTT GACTGTTCTC GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC 
    AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT GAGTTCAGAT GTGAAGCTCA AGGAGACCCC 
    GTGCCCACCA TCCGATGGAG GAAAGAGGAC GGAGAACTGC CCAAGGGAAG GTATGAGATC 
    TGGGAGGACC ACACTCTCAA ACTGAAGAGG CTGACTTCAG GAGACGCCGG TACTTACACC 
    TGTCAGGCGG AGAACATGAT GGGCAAGACG GAGGCCAGCG CCACCCTCAC CGTCCACGTT 
    GTGTCTGTTC CTCCAGTGTT TGTGGTCCGA CCCAGGAATC AGGTGGCGGG TATGGGCCGC 
    ACCGTCACCT TTCAGTGTGA AGCCACAGGA AGTCCTCAGC CTGCAATATT CTGGCAGAAA 
    GAAGGCAGTC AGAATCTCCT GTTCTCGTCT CAGCCTCCTC CTCCGTCCAG CAGATTCTCT 
    GTGTCTCAGA CTGGAGATCT GACCATCACA GCAGTTGAGC GTTCAGATGG AGGATATTAT 
    AGCTGTCAGG CTCTCAACAT CGCTGGCAGT GTGATCACCA AAGCCCTGCT GGAGGTTACA 
    GACGTGATTT CAGACAGGCC TCCACCAATC GTCCGTCAGG GTCCAACCAA CCAGACTGTG 
    GCAGTGGACG GGACGGTGGT TCTGAGCTGC CAAGCCACCG GGACACCGAT TCCTACCATC 
    TTATGGAGGA AAGATGGAGT TCTGGTGTCC ACACATGATT CCCGACTCAA GCAGCTGGAC 
    ACTGGAGCCC TGCAGATTCG ATACGCAAAG CTGGGCGACA CTGGCATGTA CACCTGCACG 
    GCTTCCACCC CGAGCGGAGA GGCGTCCTGG AAGGCCTACC TGGAGGTGCA AGAGTTTGGT 
    GCTCCAGTTC AACCGGGCCG TCCCACAGAT CCCAACCTCA TCCCCAGCGC CCCATCTAAA 
    CCAGACGTCA CGGATGTCAG CCGCACCTCC GTGTCTCTTT CCTGGAAGCC GAATCTGAAC 
    GCTGGAGCCA CACCCACCTC ATACGTCATT GAGGCCTTCA GCCATGCAGC AGGCAGCAGC 
    TGGCAGACGC TCGCCGATCA TGTGAAGACG GAAAGCTTCG TGCTTAAGGG TCTGAAGCCA 
    AGTGCCGTCT ACCTGTTTCT GGTGAGAGCG GCCAATGCGT ACGGCCTGAG CGACCCCAGT 
    CCCATCAGCG ACGCTGTCAA AACACAAGAC GTCCCACCAA CCAGCCAGGG AGTGGAGCAT 
    GGCCAGATCC AGAGAGAACT GGGAGATGTC GTCATTCACC TTCACAATCC CACAATTCTC 
    TCATCGTCTT CCGTCAGAGT CCAGTGGACT GTGGAGCAGC AGTCTCAGTA TATTCAGGGC 
    TATAAGGTGA TGTACCGTCC GTCTCCAGAA GGCGCTCCGC AGCGAGTGGA TTGGGCCATG 
    TTTGAGGTCG GAGCCCCGGG GGAGCACAGC GCTGTCGTCA CCCAGCTCAA GAAGGGCATC 
    ACCTATGAGT TCAAAGTGCG CCCTTTCTTC AATGAGTTCC AGGGCACCGA CAGCGACATC 
    AAAGTGGGAA AGACCCTGGA GGAGGCTCCC AGCGCTGCTC CCAGAGGCGT CACCGTAACA 
    GGAAGTGGAG ACAACGGCAC AGCAGTCCTG GTCGCCTGGC AACCGCCGCC AGAGGAGGAG 
    CAGAACGGCG TTGTGCAGGA GTACAAGATC TGGTGTCTTG GTAATGAAAG CCGATACCAC 
    ATCAACCGCA CAGTGGATGG TTCTACACAC TCGGTGTTGA TTCCTGGTCT GGTGGCAGGA 
    GTCACATACA GGCTAGAGGT GGCAGCAGGC ACTGGGGCCG GACCCGGGGT CAAGAGCGAC 
    CCTACGTCCT TCCAGCTTGA CTCTTCTGGC CGTGTTCTCC CGCCGCCGCT GGACGACGAT 
    AACACCATTT CCCAGCAGAT CTCAGACGTG GTGCGTCAGC CGGCTTTCAT CGCGGGCATC 
    GGCGCCGCCT GCTGGATCAT CCTGATGGTG TTCAGCATCT GGCTATACCG CCACCGCAAG 
    AAGAGGAACG GCCTCAGCAG CAGCTACGCC GGCATCCGCA AAGTGCCCTC CTTCACCTTC 
    ACCCCCACAG TGGCCTATCA GAGAGGCGGA GAGGCCGTCA GCAGTGCAGG AAGGCCGGGT 
    TTGTTGAATA TAGGTGAACC AGCTACACAG CCCTGGCTTG CGGACACCTG GCCCAACAGC 
    TGCTCCAATC ACAATGACTG CACTATTAAC TGCTGCACCG GCAGCAATGG AAACAGCGAC 
    AGCAACCTGG CAACTTACAG CCGCCCAGCC GACTGCATCG CCAACTACAG CAACCAGATG 
    GACAACAAGG CCACCAACCT GCTGGCTCCA GATACTGGGC TTTACAGCGA CGTTGACCTT 
    TCAAACAAGA TCAACGAGAT GAAGACCTTC AACAGCCCCA ACCTGAAGGA CGGGCGTTTT 
    ATGGGGCCTG GTGGTCAACC CACACCCTAC GCCACCACTC AGCTCATTCA ATCCAGCCTG 
    GCCAACAACA ACATGAACAG CAGCAGTGGC GGAAGCGGAG GAGGAAGCGC TGACTTTAAT 
    GAGAAGCAGA GCTGGAAGAG CAGCCAGGGA ACACCTCAGA AACAGGCCGA CATCAACACA 
    CCACTGCAGT ATAACGTCAT GGAACAAAAC AAACTCAACA AGGACCACTA CCGAGGTGAG 
    GGTCCCATCT CAGTCACCAT CCCTTATAAC CAGGCCCAGG ACGTCCTCAC AGGAGAAACC 
    TACAGCAGCT CGGACAGAGG GAGCAGCACC TCTGGAAGTC AAGGGCAGAA AAAGGGTGGA 
    CGAACAACTA AACAAGCCAA ACAGAATCCA ATGAATTGGA CTGAACTGTT GCCTCCTCCT 
    CCGGTTAATC CGCCGCCATG TCAAGAATAC ACCCTGGTGA TGGACGACAG TTACGACACA 
    GACCTGCAGT GCCCTTTGCC GCCGTCTCGG ATGTATCTGC AACCTGACGA GTTGGAAGAG 
    GAGGAGGACG AGGTGGAGCT GGAGCGTGGA CCTACACCTC CTGTACGGGG AGCTGCTTCT 
    TCACCTGCTG CCGTGTCCTA CAGCCACCAG TCCACCGCAA CCCTAACCCC GTCCCCTCAG 
    GAGGAGATGC ATCCCATGCT GCAGGACAAC CCTGATCTAC ATGAGCGCAG GAGACTGTTA 
    GTGAGTCCAC CTCCTCCACC ACGACCTCTC TCTCCTCCAC ACACATACGG CTACATCTCC 
    AGTCCCCTCG GCCTGGACAC GGACGGCCTA GAGGAAGAGG ATGATGATGA AGAAGAGGGT 
    GACGAAACGG ACGCAGAGGT AGCTCGAGTC CACCAGCACC ACATGATGCA CCCTCACCAG 
    CAGCACTTGC CCCAGCAGAG GGCTCTCCTG CGGGGTCTGG AACAAACCCC GGCCTCCAGC 
    ACCTGTGACC TGGAGAGCTC TGTGACTGGG TCTATGATTA ACGGATGGGG TTCGGCTTCA 
    GAAGAGGACA ACGCCTCGTC AGGCCGTTCC AGTGCAGTCA GCTCATCGGA TGGATCGTTC 
    TTTACCGATG CCGATTTCGC CCAGGCTGTG GCAGCTGCGG CTGAATATGC TGGTCTTCGA 
    GTGGCAAAGC ATCCTGGGAA AGGACATCAT CAGCTTCCAG TGTCTGGAGC AGCACGGAAA 
    TACCAAAACC TCCCTGCGGG ACACCGTCCA GGCAGCCCAG TGTCCACAGA CAGTAACGTT 
    AGCGCAGCCC CGGGTCAGAG GAGGCCCCTG AAGAGGCAAA AACAACATCC AGCTGCTCAG 
    ACAGGCTACC AGCGCCGAGA GGCCTACACA GAGGATCTCC CCCCTCCTCC GATTCCTCCT 
    CCAGCCATGC TGAAGTCTCC AACACACCCG GCCAAAACCA TCCCCGAGGG TCCACGGGGG 
    GCCATGTCGC CAAGATCCAG CTCTGCGGAT GGCAAGGACA GGCGAACGGG TGCTGGCGGG 
    CCCAGGGTCA GGGACGGTTC AGACACTCGG ACCAGCTCTT CAGAGCGGAG GGATGGCCAA 
    GATGTACAGA GGGGTCAGAG AACGGGCAAG AATAGCAAAC AAGATGAAGC CGTGAAGAGC 
    CGCCAGCACC CAGGCTCAGA GGACATCCTA CCTTACAGTC GTCCTTCTTT CCCAGCCGTC 
    CACAGTCCTC GAGGAGAACG GGACCCCAGT TCCTCCAGTT CACTGTCCTC TCGGGGGTCT 
    GGCGGGCGAC GGAGAGGTGA AGGGCCACCA GGGGCCCGCA GAAACCCTGC AGACATTGGG 
    CTAAACACCA TAGGAGCCTT CCAGACTGGA GGGGACGAGC TTGAGATGGT GGAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337006.1
    CDS245..5224
    Translation

    Target ORF information:

    RefSeq Version XM_021481331.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021481331.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGGGCAG GGCTGCTGTT TCTCATCTTC CTCCTCCTGG ATCTTGGGAT CTGCTGGGAT 
    GGAGACAAGC CTGGGAGAAA AAAGGGATCC CGTCTGCGGC AGGAAGATTA CGCACCCCGT 
    ATTGTTGAAC ACCCCTCTGA TCTGATCGTA TCCAAAGGCG AACCGGCCAC CCTAAACTGC 
    AAGGCTGAAG GACGTCCCAC GCCCACAGTG GAGTGGTACA AAGATGGAGA ACGAGTTGAA 
    ACCGACCGGG ACAATCCTCG ATCCCACCGC ATGCTCCTGC CCACCGGATC TCTCTTCTTT 
    CTCCGCATTG TTCACGGCCG GCGCAGCAAA CCCGACGAAG GCTCCTACGT TTGTGTGGCA 
    CGTAACTATC TGGGAGAAGC CGTCAGCCAC AACGCCTCGC TGGAAGTAGC TATTCTCCGA 
    GATGATTTCC GACAGAACCC TGTGGACGTG ATGGTGGCAG CGGGGGAGCC AGCTGTAATG 
    GAGTGCCAGC CGCCCCGAGG ACACCCAGAG CCCACCATCT CCTGGAAGAA AGATGGGGTC 
    AATATAGACG ACAGAGACGA GAGGATAACG ATACGAGGAG GAAAGCTCAT GATCACCAAC 
    ACCAGAAAAA CGGACGCGGG GAAGTACGTG TGCGTGGGAA CCAACATGAT GGGGGAACGA 
    GAGAGCGAGA TCGCGGAGTT GACTGTTCTC GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC 
    AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT GAGTTCAGAT GTGAAGCTCA AGGAGACCCC 
    GTGCCCACCA TCCGATGGAG GAAAGAGGAC GGAGAACTGC CCAAGGGAAG GTATGAGATC 
    TGGGAGGACC ACACTCTCAA ACTGAAGAGG CTGACTTCAG GAGACGCCGG TACTTACACC 
    TGTCAGGCGG AGAACATGAT GGGCAAGACG GAGGCCAGCG CCACCCTCAC CGTCCACGTT 
    GTGTCTGTTC CTCCAGTGTT TGTGGTCCGA CCCAGGAATC AGGTGGCGGG TATGGGCCGC 
    ACCGTCACCT TTCAGTGTGA AGCCACAGGA AGTCCTCAGC CTGCAATATT CTGGCAGAAA 
    GAAGGCAGTC AGAATCTCCT GTTCTCGTCT CAGCCTCCTC CTCCGTCCAG CAGATTCTCT 
    GTGTCTCAGA CTGGAGATCT GACCATCACA GCAGTTGAGC GTTCAGATGG AGGATATTAT 
    AGCTGTCAGG CTCTCAACAT CGCTGGCAGT GTGATCACCA AAGCCCTGCT GGAGGTTACA 
    GACGTGATTT CAGACAGGCC TCCACCAATC GTCCGTCAGG GTCCAACCAA CCAGACTGTG 
    GCAGTGGACG GGACGGTGGT TCTGAGCTGC CAAGCCACCG GGACACCGAT TCCTACCATC 
    TTATGGAGGA AAGATGGAGT TCTGGTGTCC ACACATGATT CCCGACTCAA GCAGCTGGAC 
    ACTGGAGCCC TGCAGATTCG ATACGCAAAG CTGGGCGACA CTGGCATGTA CACCTGCACG 
    GCTTCCACCC CGAGCGGAGA GGCGTCCTGG AAGGCCTACC TGGAGGTGCA AGAGTTTGGT 
    GCTCCAGTTC AACCGGGCCG TCCCACAGAT CCCAACCTCA TCCCCAGCGC CCCATCTAAA 
    CCAGACGTCA CGGATGTCAG CCGCACCTCC GTGTCTCTTT CCTGGAAGCC GAATCTGAAC 
    GCTGGAGCCA CACCCACCTC ATACGTCATT GAGGCCTTCA GCCATGCAGC AGGCAGCAGC 
    TGGCAGACGC TCGCCGATCA TGTGAAGACG GAAAGCTTCG TGCTTAAGGG TCTGAAGCCA 
    AGTGCCGTCT ACCTGTTTCT GGTGAGAGCG GCCAATGCGT ACGGCCTGAG CGACCCCAGT 
    CCCATCAGCG ACGCTGTCAA AACACAAGAC GTCCCACCAA CCAGCCAGGG AGTGGAGCAT 
    GGCCAGATCC AGAGAGAACT GGGAGATGTC GTCATTCACC TTCACAATCC CACAATTCTC 
    TCATCGTCTT CCGTCAGAGT CCAGTGGACT GTGGAGCAGC AGTCTCAGTA TATTCAGGGC 
    TATAAGGTGA TGTACCGTCC GTCTCCAGAA GGCGCTCCGC AGCGAGTGGA TTGGGCCATG 
    TTTGAGGTCG GAGCCCCGGG GGAGCACAGC GCTGTCGTCA CCCAGCTCAA GAAGGGCATC 
    ACCTATGAGT TCAAAGTGCG CCCTTTCTTC AATGAGTTCC AGGGCACCGA CAGCGACATC 
    AAAGTGGGAA AGACCCTGGA GGAGGCTCCC AGCGCTGCTC CCAGAGGCGT CACCGTAACA 
    GGAAGTGGAG ACAACGGCAC AGCAGTCCTG GTCGCCTGGC AACCGCCGCC AGAGGAGGAG 
    CAGAACGGCG TTGTGCAGGA GTACAAGATC TGGTGTCTTG GTAATGAAAG CCGATACCAC 
    ATCAACCGCA CAGTGGATGG TTCTACACAC TCGGTGTTGA TTCCTGGTCT GGTGGCAGGA 
    GTCACATACA GGCTAGAGGT GGCAGCAGGC ACTGGGGCCG GACCCGGGGT CAAGAGCGAC 
    CCTACGTCCT TCCAGCTTGA CTCTTCTGGC CGTGTTCTCC CGCCGCCGCT GGACGACGAT 
    AACACCATTT CCCAGCAGAT CTCAGACGTG GTGCGTCAGC CGGCTTTCAT CGCGGGCATC 
    GGCGCCGCCT GCTGGATCAT CCTGATGGTG TTCAGCATCT GGCTATACCG CCACCGCAAG 
    AAGAGGAACG GCCTCAGCAG CAGCTACGCC GGCATCCGCA AAGTGCCCTC CTTCACCTTC 
    ACCCCCACAG TGGCCTATCA GAGAGGCGGA GAGGCCGTCA GCAGTGCAGG AAGGCCGGGT 
    TTGTTGAATA TAGGTGAACC AGCTACACAG CCCTGGCTTG CGGACACCTG GCCCAACAGC 
    TGCTCCAATC ACAATGACTG CACTATTAAC TGCTGCACCG GCAGCAATGG AAACAGCGAC 
    AGCAACCTGG CAACTTACAG CCGCCCAGCC GACTGCATCG CCAACTACAG CAACCAGATG 
    GACAACAAGG CCACCAACCT GCTGGCTCCA GATACTGGGC TTTACAGCGA CGTTGACCTT 
    TCAAACAAGA TCAACGAGAT GAAGACCTTC AACAGCCCCA ACCTGAAGGA CGGGCGTTTT 
    ATGGGGCCTG GTGGTCAACC CACACCCTAC GCCACCACTC AGCTCATTCA ATCCAGCCTG 
    GCCAACAACA ACATGAACAG CAGCAGTGGC GGAAGCGGAG GAGGAAGCGC TGACTTTAAT 
    GAGAAGCAGA GCTGGAAGAG CAGCCAGGGA ACACCTCAGA AACAGGCCGA CATCAACACA 
    CCACTGCAGT ATAACGTCAT GGAACAAAAC AAACTCAACA AGGACCACTA CCGAGGTGAG 
    GGTCCCATCT CAGTCACCAT CCCTTATAAC CAGGCCCAGG ACGTCCTCAC AGGAGAAACC 
    TACAGCAGCT CGGACAGAGG GAGCAGCACC TCTGGAAGTC AAGGGCAGAA AAAGGGTGGA 
    CGAACAACTA AACAAGCCAA ACAGAATCCA ATGAATTGGA CTGAACTGTT GCCTCCTCCT 
    CCGGTTAATC CGCCGCCATG TCAAGAATAC ACCCTGGTGA TGGACGACAG TTACGACACA 
    GACCTGCAGT GCCCTTTGCC GCCGTCTCGG ATGTATCTGC AACCTGACGA GTTGGAAGAG 
    GAGGAGGACG AGGTGGAGCT GGAGCGTGGA CCTACACCTC CTGTACGGGG AGCTGCTTCT 
    TCACCTGCTG CCGTGTCCTA CAGCCACCAG TCCACCGCAA CCCTAACCCC GTCCCCTCAG 
    GAGGAGATGC ATCCCATGCT GCAGGACAAC CCTGATCTAC ATGAGCGCAG GAGACTGTTA 
    GTGAGTCCAC CTCCTCCACC ACGACCTCTC TCTCCTCCAC ACACATACGG CTACATCTCC 
    AGTCCCCTCG GCCTGGACAC GGACGGCCTA GAGGAAGAGG ATGATGATGA AGAAGAGGGT 
    GACGAAACGG ACGCAGAGGT AGCTCGAGTC CACCAGCACC ACATGATGCA CCCTCACCAG 
    CAGCACTTGC CCCAGCAGAG GGCTCTCCTG CGGGGTCTGG AACAAACCCC GGCCTCCAGC 
    ACCTGTGACC TGGAGAGCTC TGTGACTGGG TCTATGATTA ACGGATGGGG TTCGGCTTCA 
    GAAGAGGACA ACGCCTCGTC AGGCCGTTCC AGTGCAGTCA GCTCATCGGA TGGATCGTTC 
    TTTACCGATG CCGATTTCGC CCAGGCTGTG GCAGCTGCGG CTGAATATGC TGGTCTTCGA 
    GTGGCAAAGC ATCCTGGGAA AGGACATCAT CAGCTTCCAG TGTCTGGAGC AGCACGGAAA 
    TACCAAAACC TCCCTGCGGG ACACCGTCCA GGCAGCCCAG TGTCCACAGA CAGTAACGTT 
    AGCGCAGCCC CGGGTCAGAG GAGGCCCCTG AAGAGGCAAA AACAACATCC AGCTGCTCAG 
    ACAGGCTACC AGCGCCGAGA GGCCTACACA GAGGATCTCC CCCCTCCTCC GATTCCTCCT 
    CCAGCCATGC TGAAGTCTCC AACACACCCG GCCAAAACCA TCCCCGAGGG TCCACGGGGG 
    GCCATGTCGC CAAGATCCAG CTCTGCGGAT GGCAAGGACA GGCGAACGGG TGCTGGCGGG 
    CCCAGGGTCA GGGACGGTTC AGACACTCGG ACCAGCTCTT CAGAGCGGAG GGATGGCCAA 
    GATGTACAGA GGGGTCAGAG AACGGGCAAG AATAGCAAAC AAGATGAAGC CGTGAAGAGC 
    CGCCAGCACC CAGGCTCAGA GGACATCCTA CCTTACAGTC GTCCTTCTTT CCCAGCCGTC 
    CACAGTCCTC GAGGAGAACG GGACCCCAGT TCCTCCAGTT CACTGTCCTC TCGGGGGTCT 
    GGCGGGCGAC GGAGAGGTGA AGGGCCACCA GGGGCCCGCA GAAACCCTGC AGACATTGGG 
    CTAAACACCA TAGGAGCCTT CCAGACTGGA GGGGACGAGC TTGAGATGGT GGAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_021481332.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337007.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID ODa148919
    Clone ID Related Accession (Same CDS sequence) XM_021481332.1
    Accession Version XM_021481332.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTGCAGA TGGCCCGATA CACTTTACTG GAGGACTCAA AGTCTGTATT ACTTCCAACA 
    GACACGGGGA CTCAGGATGG GACTAGCAAT AGGGCCACAG AGACCCCCAC TGCAGAACAC 
    GTTGACGACA ACTCTCTGGG CTACACAGGA TCCCGTCTGC GGCAGGAAGA TTACGCACCC 
    CGTATTGTTG AACACCCCTC TGATCTGATC GTATCCAAAG GCGAACCGGC CACCCTAAAC 
    TGCAAGGCTG AAGGACGTCC CACGCCCACA GTGGAGTGGT ACAAAGATGG AGAACGAGTT 
    GAAACCGACC GGGACAATCC TCGATCCCAC CGCATGCTCC TGCCCACCGG ATCTCTCTTC 
    TTTCTCCGCA TTGTTCACGG CCGGCGCAGC AAACCCGACG AAGGCTCCTA CGTTTGTGTG 
    GCACGTAACT ATCTGGGAGA AGCCGTCAGC CACAACGCCT CGCTGGAAGT AGCTATTCTC 
    CGAGATGATT TCCGACAGAA CCCTGTGGAC GTGATGGTGG CAGCGGGGGA GCCAGCTGTA 
    ATGGAGTGCC AGCCGCCCCG AGGACACCCA GAGCCCACCA TCTCCTGGAA GAAAGATGGG 
    GTCAATATAG ACGACAGAGA CGAGAGGATA ACGATACGAG GAGGAAAGCT CATGATCACC 
    AACACCAGAA AAACGGACGC GGGGAAGTAC GTGTGCGTGG GAACCAACAT GATGGGGGAA 
    CGAGAGAGCG AGATCGCGGA GTTGACTGTT CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG 
    AGCAGCCAGT CCGTCCTTGT GGATCAGAGC GTTGAGTTCA GATGTGAAGC TCAAGGAGAC 
    CCCGTGCCCA CCATCCGATG GAGGAAAGAG GACGGAGAAC TGCCCAAGGG AAGGTATGAG 
    ATCTGGGAGG ACCACACTCT CAAACTGAAG AGGCTGACTT CAGGAGACGC CGGTACTTAC 
    ACCTGTCAGG CGGAGAACAT GATGGGCAAG ACGGAGGCCA GCGCCACCCT CACCGTCCAC 
    GTTGTGTCTG TTCCTCCAGT GTTTGTGGTC CGACCCAGGA ATCAGGTGGC GGGTATGGGC 
    CGCACCGTCA CCTTTCAGTG TGAAGCCACA GGAAGTCCTC AGCCTGCAAT ATTCTGGCAG 
    AAAGAAGGCA GTCAGAATCT CCTGTTCTCG TCTCAGCCTC CTCCTCCGTC CAGCAGATTC 
    TCTGTGTCTC AGACTGGAGA TCTGACCATC ACAGCAGTTG AGCGTTCAGA TGGAGGATAT 
    TATAGCTGTC AGGCTCTCAA CATCGCTGGC AGTGTGATCA CCAAAGCCCT GCTGGAGGTT 
    ACAGACGTGA TTTCAGACAG GCCTCCACCA ATCGTCCGTC AGGGTCCAAC CAACCAGACT 
    GTGGCAGTGG ACGGGACGGT GGTTCTGAGC TGCCAAGCCA CCGGGACACC GATTCCTACC 
    ATCTTATGGA GGAAAGATGG AGTTCTGGTG TCCACACATG ATTCCCGACT CAAGCAGCTG 
    GACACTGGAG CCCTGCAGAT TCGATACGCA AAGCTGGGCG ACACTGGCAT GTACACCTGC 
    ACGGCTTCCA CCCCGAGCGG AGAGGCGTCC TGGAAGGCCT ACCTGGAGGT GCAAGAGTTT 
    GGTGCTCCAG TTCAACCGGG CCGTCCCACA GATCCCAACC TCATCCCCAG CGCCCCATCT 
    AAACCAGACG TCACGGATGT CAGCCGCACC TCCGTGTCTC TTTCCTGGAA GCCGAATCTG 
    AACGCTGGAG CCACACCCAC CTCATACGTC ATTGAGGCCT TCAGCCATGC AGCAGGCAGC 
    AGCTGGCAGA CGCTCGCCGA TCATGTGAAG ACGGAAAGCT TCGTGCTTAA GGGTCTGAAG 
    CCAAGTGCCG TCTACCTGTT TCTGGTGAGA GCGGCCAATG CGTACGGCCT GAGCGACCCC 
    AGTCCCATCA GCGACGCTGT CAAAACACAA GACGTCCCAC CAACCAGCCA GGGAGTGGAG 
    CATGGCCAGA TCCAGAGAGA ACTGGGAGAT GTCGTCATTC ACCTTCACAA TCCCACAATT 
    CTCTCATCGT CTTCCGTCAG AGTCCAGTGG ACTGTGGAGC AGCAGTCTCA GTATATTCAG 
    GGCTATAAGG TGATGTACCG TCCGTCTCCA GAAGGCGCTC CGCAGCGAGT GGATTGGGCC 
    ATGTTTGAGG TCGGAGCCCC GGGGGAGCAC AGCGCTGTCG TCACCCAGCT CAAGAAGGGC 
    ATCACCTATG AGTTCAAAGT GCGCCCTTTC TTCAATGAGT TCCAGGGCAC CGACAGCGAC 
    ATCAAAGTGG GAAAGACCCT GGAGGAGGCT CCCAGCGCTG CTCCCAGAGG CGTCACCGTA 
    ACAGGAAGTG GAGACAACGG CACAGCAGTC CTGGTCGCCT GGCAACCGCC GCCAGAGGAG 
    GAGCAGAACG GCGTTGTGCA GGAGTACAAG ATCTGGTGTC TTGGTAATGA AAGCCGATAC 
    CACATCAACC GCACAGTGGA TGGTTCTACA CACTCGGTGT TGATTCCTGG TCTGGTGGCA 
    GGAGTCACAT ACAGGCTAGA GGTGGCAGCA GGCACTGGGG CCGGACCCGG GGTCAAGAGC 
    GACCCTACGT CCTTCCAGCT TGACTCTTCT GGCCGTGTTC TCCCGCCGCC GCTGGACGAC 
    GATAACACCA TTTCCCAGCA GATCTCAGAC GTGGTGCGTC AGCCGGCTTT CATCGCGGGC 
    ATCGGCGCCG CCTGCTGGAT CATCCTGATG GTGTTCAGCA TCTGGCTATA CCGCCACCGC 
    AAGAAGAGGA ACGGCCTCAG CAGCAGCTAC GCCGGCATCC GCAAAGTGCC CTCCTTCACC 
    TTCACCCCCA CAGTGGCCTA TCAGAGAGGC GGAGAGGCCG TCAGCAGTGC AGGAAGGCCG 
    GGTTTGTTGA ATATAGGTGA ACCAGCTACA CAGCCCTGGC TTGCGGACAC CTGGCCCAAC 
    AGCTGCTCCA ATCACAATGA CTGCACTATT AACTGCTGCA CCGGCAGCAA TGGAAACAGC 
    GACAGCAACC TGGCAACTTA CAGCCGCCCA GACCACTACC GAGGTGAGGG TCCCATCTCA 
    GTCACCATCC CTTATAACCA GGCCCAGGAC GTCCTCACAG GAGAAACCTA CAGCAGCTCG 
    GACAGAGGGA GCAGCACCTC TGGAAGTCAA GGGCAGAAAA AGGGTGGACG AACAACTAAA 
    CAAGCCAAAC AGAATCCAAT GAATTGGACT GAACTGTTGC CTCCTCCTCC GGTTAATCCG 
    CCGCCATGTC AAGAATACAC CCTGGTGATG GACGACAGTT ACGACACAGA CCTGCAGTGC 
    CCTTTGCCGC CGTCTCGGAT GTATCTGCAA CCTGACGAGT TGGAAGAGGA GGAGGACGAG 
    GTGGAGCTGG AGCGTGGACC TACACCTCCT GTACGGGGAG CTGCTTCTTC ACCTGCTGCC 
    GTGTCCTACA GCCACCAGTC CACCGCAACC CTAACCCCGT CCCCTCAGGA GGAGATGCAT 
    CCCATGCTGC AGGACAACCC TGATCTACAT GAGCGCAGGA GACTGTTAGT GAGTCCACCT 
    CCTCCACCAC GACCTCTCTC TCCTCCACAC ACATACGGCT ACATCTCCAG TCCCCTCGGC 
    CTGGACACGG ACGGCCTAGA GGAAGAGGAT GATGATGAAG AAGAGGGTGA CGAAACGGAC 
    GCAGAGGTAG CTCGAGTCCA CCAGCACCAC ATGATGCACC CTCACCAGCA GCACTTGCCC 
    CAGCAGAGGG CTCTCCTGCG GGGTCTGGAA CAAACCCCGG CCTCCAGCAC CTGTGACCTG 
    GAGAGCTCTG TGACTGGGTC TATGATTAAC GGATGGGGTT CGGCTTCAGA AGAGGACAAC 
    GCCTCGTCAG GCCGTTCCAG TGCAGTCAGC TCATCGGATG GATCGTTCTT TACCGATGCC 
    GATTTCGCCC AGGCTGTGGC AGCTGCGGCT GAATATGCTG GTCTTCGAGT GGCAAAGCAT 
    CCTGGGAAAG GACATCATCA GCTTCCAGTG TCTGGAGCAG CACGGAAATA CCAAAACCTC 
    CCTGCGGGAC ACCGTCCAGG CAGCCCAGTG TCCACAGACA GTAACGTTAG CGCAGCCCCG 
    GGTCAGAGGA GGCCCCTGAA GAGGCAAAAA CAACATCCAG CTGCTCAGAC AGGCTACCAG 
    CGCCGAGAGG CCTACACAGA GGATCTCCCC CCTCCTCCGA TTCCTCCTCC AGCCATGCTG 
    AAGTCTCCAA CACACCCGGC CAAAACCATC CCCGAGGGTC CACGGGGGGC CATGTCGCCA 
    AGATCCAGCT CTGCGGATGG CAAGGACAGG CGAACGGGTG CTGGCGGGCC CAGGGTCAGG 
    GACGGTTCAG ACACTCGGAC CAGCTCTTCA GAGCGGAGGG ATGGCCAAGA TGTACAGAGG 
    GGTCAGAGAA CGGGCAAGAA TAGCAAACAA GATGAAGCCG TGAAGAGCCG CCAGCACCCA 
    GGCTCAGAGG ACATCCTACC TTACAGTCGT CCTTCTTTCC CAGCCGTCCA CAGTCCTCGA 
    GGAGAACGGG ACCCCAGTTC CTCCAGTTCA CTGTCCTCTC GGGGGTCTGG CGGGCGACGG 
    AGAGGTGAAG GGCCACCAGG GGCCCGCAGA AACCCTGCAG ACATTGGGCT AAACACCATA 
    GGAGCCTTCC AGACTGGAGG GGACGAGCTT GAGATGGTGG AGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337007.1
    CDS7..4674
    Translation

    Target ORF information:

    RefSeq Version XM_021481332.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021481332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTGCAGA TGGCCCGATA CACTTTACTG GAGGACTCAA AGTCTGTATT ACTTCCAACA 
    GACACGGGGA CTCAGGATGG GACTAGCAAT AGGGCCACAG AGACCCCCAC TGCAGAACAC 
    GTTGACGACA ACTCTCTGGG CTACACAGGA TCCCGTCTGC GGCAGGAAGA TTACGCACCC 
    CGTATTGTTG AACACCCCTC TGATCTGATC GTATCCAAAG GCGAACCGGC CACCCTAAAC 
    TGCAAGGCTG AAGGACGTCC CACGCCCACA GTGGAGTGGT ACAAAGATGG AGAACGAGTT 
    GAAACCGACC GGGACAATCC TCGATCCCAC CGCATGCTCC TGCCCACCGG ATCTCTCTTC 
    TTTCTCCGCA TTGTTCACGG CCGGCGCAGC AAACCCGACG AAGGCTCCTA CGTTTGTGTG 
    GCACGTAACT ATCTGGGAGA AGCCGTCAGC CACAACGCCT CGCTGGAAGT AGCTATTCTC 
    CGAGATGATT TCCGACAGAA CCCTGTGGAC GTGATGGTGG CAGCGGGGGA GCCAGCTGTA 
    ATGGAGTGCC AGCCGCCCCG AGGACACCCA GAGCCCACCA TCTCCTGGAA GAAAGATGGG 
    GTCAATATAG ACGACAGAGA CGAGAGGATA ACGATACGAG GAGGAAAGCT CATGATCACC 
    AACACCAGAA AAACGGACGC GGGGAAGTAC GTGTGCGTGG GAACCAACAT GATGGGGGAA 
    CGAGAGAGCG AGATCGCGGA GTTGACTGTT CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG 
    AGCAGCCAGT CCGTCCTTGT GGATCAGAGC GTTGAGTTCA GATGTGAAGC TCAAGGAGAC 
    CCCGTGCCCA CCATCCGATG GAGGAAAGAG GACGGAGAAC TGCCCAAGGG AAGGTATGAG 
    ATCTGGGAGG ACCACACTCT CAAACTGAAG AGGCTGACTT CAGGAGACGC CGGTACTTAC 
    ACCTGTCAGG CGGAGAACAT GATGGGCAAG ACGGAGGCCA GCGCCACCCT CACCGTCCAC 
    GTTGTGTCTG TTCCTCCAGT GTTTGTGGTC CGACCCAGGA ATCAGGTGGC GGGTATGGGC 
    CGCACCGTCA CCTTTCAGTG TGAAGCCACA GGAAGTCCTC AGCCTGCAAT ATTCTGGCAG 
    AAAGAAGGCA GTCAGAATCT CCTGTTCTCG TCTCAGCCTC CTCCTCCGTC CAGCAGATTC 
    TCTGTGTCTC AGACTGGAGA TCTGACCATC ACAGCAGTTG AGCGTTCAGA TGGAGGATAT 
    TATAGCTGTC AGGCTCTCAA CATCGCTGGC AGTGTGATCA CCAAAGCCCT GCTGGAGGTT 
    ACAGACGTGA TTTCAGACAG GCCTCCACCA ATCGTCCGTC AGGGTCCAAC CAACCAGACT 
    GTGGCAGTGG ACGGGACGGT GGTTCTGAGC TGCCAAGCCA CCGGGACACC GATTCCTACC 
    ATCTTATGGA GGAAAGATGG AGTTCTGGTG TCCACACATG ATTCCCGACT CAAGCAGCTG 
    GACACTGGAG CCCTGCAGAT TCGATACGCA AAGCTGGGCG ACACTGGCAT GTACACCTGC 
    ACGGCTTCCA CCCCGAGCGG AGAGGCGTCC TGGAAGGCCT ACCTGGAGGT GCAAGAGTTT 
    GGTGCTCCAG TTCAACCGGG CCGTCCCACA GATCCCAACC TCATCCCCAG CGCCCCATCT 
    AAACCAGACG TCACGGATGT CAGCCGCACC TCCGTGTCTC TTTCCTGGAA GCCGAATCTG 
    AACGCTGGAG CCACACCCAC CTCATACGTC ATTGAGGCCT TCAGCCATGC AGCAGGCAGC 
    AGCTGGCAGA CGCTCGCCGA TCATGTGAAG ACGGAAAGCT TCGTGCTTAA GGGTCTGAAG 
    CCAAGTGCCG TCTACCTGTT TCTGGTGAGA GCGGCCAATG CGTACGGCCT GAGCGACCCC 
    AGTCCCATCA GCGACGCTGT CAAAACACAA GACGTCCCAC CAACCAGCCA GGGAGTGGAG 
    CATGGCCAGA TCCAGAGAGA ACTGGGAGAT GTCGTCATTC ACCTTCACAA TCCCACAATT 
    CTCTCATCGT CTTCCGTCAG AGTCCAGTGG ACTGTGGAGC AGCAGTCTCA GTATATTCAG 
    GGCTATAAGG TGATGTACCG TCCGTCTCCA GAAGGCGCTC CGCAGCGAGT GGATTGGGCC 
    ATGTTTGAGG TCGGAGCCCC GGGGGAGCAC AGCGCTGTCG TCACCCAGCT CAAGAAGGGC 
    ATCACCTATG AGTTCAAAGT GCGCCCTTTC TTCAATGAGT TCCAGGGCAC CGACAGCGAC 
    ATCAAAGTGG GAAAGACCCT GGAGGAGGCT CCCAGCGCTG CTCCCAGAGG CGTCACCGTA 
    ACAGGAAGTG GAGACAACGG CACAGCAGTC CTGGTCGCCT GGCAACCGCC GCCAGAGGAG 
    GAGCAGAACG GCGTTGTGCA GGAGTACAAG ATCTGGTGTC TTGGTAATGA AAGCCGATAC 
    CACATCAACC GCACAGTGGA TGGTTCTACA CACTCGGTGT TGATTCCTGG TCTGGTGGCA 
    GGAGTCACAT ACAGGCTAGA GGTGGCAGCA GGCACTGGGG CCGGACCCGG GGTCAAGAGC 
    GACCCTACGT CCTTCCAGCT TGACTCTTCT GGCCGTGTTC TCCCGCCGCC GCTGGACGAC 
    GATAACACCA TTTCCCAGCA GATCTCAGAC GTGGTGCGTC AGCCGGCTTT CATCGCGGGC 
    ATCGGCGCCG CCTGCTGGAT CATCCTGATG GTGTTCAGCA TCTGGCTATA CCGCCACCGC 
    AAGAAGAGGA ACGGCCTCAG CAGCAGCTAC GCCGGCATCC GCAAAGTGCC CTCCTTCACC 
    TTCACCCCCA CAGTGGCCTA TCAGAGAGGC GGAGAGGCCG TCAGCAGTGC AGGAAGGCCG 
    GGTTTGTTGA ATATAGGTGA ACCAGCTACA CAGCCCTGGC TTGCGGACAC CTGGCCCAAC 
    AGCTGCTCCA ATCACAATGA CTGCACTATT AACTGCTGCA CCGGCAGCAA TGGAAACAGC 
    GACAGCAACC TGGCAACTTA CAGCCGCCCA GACCACTACC GAGGTGAGGG TCCCATCTCA 
    GTCACCATCC CTTATAACCA GGCCCAGGAC GTCCTCACAG GAGAAACCTA CAGCAGCTCG 
    GACAGAGGGA GCAGCACCTC TGGAAGTCAA GGGCAGAAAA AGGGTGGACG AACAACTAAA 
    CAAGCCAAAC AGAATCCAAT GAATTGGACT GAACTGTTGC CTCCTCCTCC GGTTAATCCG 
    CCGCCATGTC AAGAATACAC CCTGGTGATG GACGACAGTT ACGACACAGA CCTGCAGTGC 
    CCTTTGCCGC CGTCTCGGAT GTATCTGCAA CCTGACGAGT TGGAAGAGGA GGAGGACGAG 
    GTGGAGCTGG AGCGTGGACC TACACCTCCT GTACGGGGAG CTGCTTCTTC ACCTGCTGCC 
    GTGTCCTACA GCCACCAGTC CACCGCAACC CTAACCCCGT CCCCTCAGGA GGAGATGCAT 
    CCCATGCTGC AGGACAACCC TGATCTACAT GAGCGCAGGA GACTGTTAGT GAGTCCACCT 
    CCTCCACCAC GACCTCTCTC TCCTCCACAC ACATACGGCT ACATCTCCAG TCCCCTCGGC 
    CTGGACACGG ACGGCCTAGA GGAAGAGGAT GATGATGAAG AAGAGGGTGA CGAAACGGAC 
    GCAGAGGTAG CTCGAGTCCA CCAGCACCAC ATGATGCACC CTCACCAGCA GCACTTGCCC 
    CAGCAGAGGG CTCTCCTGCG GGGTCTGGAA CAAACCCCGG CCTCCAGCAC CTGTGACCTG 
    GAGAGCTCTG TGACTGGGTC TATGATTAAC GGATGGGGTT CGGCTTCAGA AGAGGACAAC 
    GCCTCGTCAG GCCGTTCCAG TGCAGTCAGC TCATCGGATG GATCGTTCTT TACCGATGCC 
    GATTTCGCCC AGGCTGTGGC AGCTGCGGCT GAATATGCTG GTCTTCGAGT GGCAAAGCAT 
    CCTGGGAAAG GACATCATCA GCTTCCAGTG TCTGGAGCAG CACGGAAATA CCAAAACCTC 
    CCTGCGGGAC ACCGTCCAGG CAGCCCAGTG TCCACAGACA GTAACGTTAG CGCAGCCCCG 
    GGTCAGAGGA GGCCCCTGAA GAGGCAAAAA CAACATCCAG CTGCTCAGAC AGGCTACCAG 
    CGCCGAGAGG CCTACACAGA GGATCTCCCC CCTCCTCCGA TTCCTCCTCC AGCCATGCTG 
    AAGTCTCCAA CACACCCGGC CAAAACCATC CCCGAGGGTC CACGGGGGGC CATGTCGCCA 
    AGATCCAGCT CTGCGGATGG CAAGGACAGG CGAACGGGTG CTGGCGGGCC CAGGGTCAGG 
    GACGGTTCAG ACACTCGGAC CAGCTCTTCA GAGCGGAGGG ATGGCCAAGA TGTACAGAGG 
    GGTCAGAGAA CGGGCAAGAA TAGCAAACAA GATGAAGCCG TGAAGAGCCG CCAGCACCCA 
    GGCTCAGAGG ACATCCTACC TTACAGTCGT CCTTCTTTCC CAGCCGTCCA CAGTCCTCGA 
    GGAGAACGGG ACCCCAGTTC CTCCAGTTCA CTGTCCTCTC GGGGGTCTGG CGGGCGACGG 
    AGAGGTGAAG GGCCACCAGG GGCCCGCAGA AACCCTGCAG ACATTGGGCT AAACACCATA 
    GGAGCCTTCC AGACTGGAGG GGACGAGCTT GAGATGGTGG AGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359204.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214693.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa55055
    Clone ID Related Accession (Same CDS sequence) XM_017359204.1
    Accession Version XM_017359204.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCAGTTCA GAGATTGCAA TTGCAGATTT TTTATACGGA ACTGTGTCCT GCTTATGGTT 
    TGGTATCAGG GGTATTGGGG CGCCACTATG TTGCAGATGG CCCGATACAC TTTACTGGAG 
    GACTCAAAGT CTGTATTACT TCCAACAGAC ACGGGGACTC AGGATGGGAC TAGCAATAGG 
    GCCACAGAGA CCCCCACTGC AGAACACGTT GACGACAACT CTCTGGGCTA CACAGGATCC 
    CGTCTGCGGC AGGAAGATTA CGCACCCCGT ATTGTTGAAC ACCCCTCTGA TCTGATCGTA 
    TCCAAAGGCG AACCGGCCAC CCTAAACTGC AAGGCTGAAG GACGTCCCAC GCCCACAGTG 
    GAGTGGTACA AAGATGGAGA ACGAGTTGAA ACCGACCGGG ACAATCCTCG ATCCCACCGC 
    ATGCTCCTGC CCACCGGATC TCTCTTCTTT CTCCGCATTG TTCACGGCCG GCGCAGCAAA 
    CCCGACGAAG GCTCCTACGT TTGTGTGGCA CGTAACTATC TGGGAGAAGC CGTCAGCCAC 
    AACGCCTCGC TGGAAGTAGC TATTCTCCGA GATGATTTCC GACAGAACCC TGTGGACGTG 
    ATGGTGGCAG CGGGGGAGCC AGCTGTAATG GAGTGCCAGC CGCCCCGAGG ACACCCAGAG 
    CCCACCATCT CCTGGAAGAA AGATGGGGTC AATATAGACG ACAGAGACGA GAGGATAACG 
    ATACGAGGAG GAAAGCTCAT GATCACCAAC ACCAGAAAAA CGGACGCGGG GAAGTACGTG 
    TGCGTGGGAA CCAACATGAT GGGGGAACGA GAGAGCGAGA TCGCGGAGTT GACTGTTCTC 
    GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT 
    GAGTTCAGAT GTGAAGCTCA AGGAGACCCC GTGCCCACCA TCCGATGGAG GAAAGAGGAC 
    GGAGAACTGC CCAAGGGAAG GTATGAGATC TGGGAGGACC ACACTCTCAA ACTGAAGAGG 
    CTGACTTCAG GAGACGCCGG TACTTACACC TGTCAGGCGG AGAACATGAT GGGCAAGACG 
    GAGGCCAGCG CCACCCTCAC CGTCCACGTT GTGTCTGTTC CTCCAGTGTT TGTGGTCCGA 
    CCCAGGAATC AGGTGGCGGG TATGGGCCGC ACCGTCACCT TTCAGTGTGA AGCCACAGGA 
    AGTCCTCAGC CTGCAATATT CTGGCAGAAA GAAGGCAGTC AGAATCTCCT GTTCTCGTCT 
    CAGCCTCCTC CTCCGTCCAG CAGATTCTCT GTGTCTCAGA CTGGAGATCT GACCATCACA 
    GCAGTTGAGC GTTCAGATGG AGGATATTAT AGCTGTCAGG CTCTCAACAT CGCTGGCAGT 
    GTGATCACCA AAGCCCTGCT GGAGGTTACA GACGTGATTT CAGACAGGCC TCCACCAATC 
    GTCCGTCAGG GTCCAACCAA CCAGACTGTG GCAGTGGACG GGACGGTGGT TCTGAGCTGC 
    CAAGCCACCG GGACACCGAT TCCTACCATC TTATGGAGGA AAGATGGAGT TCTGGTGTCC 
    ACACATGATT CCCGACTCAA GCAGCTGGAC ACTGGAGCCC TGCAGATTCG ATACGCAAAG 
    CTGGGCGACA CTGGCATGTA CACCTGCACG GCTTCCACCC CGAGCGGAGA GGCGTCCTGG 
    AAGGCCTACC TGGAGGTGCA AGAGTTTGGT GCTCCAGTTC AACCGGGCCG TCCCACAGAT 
    CCCAACCTCA TCCCCAGCGC CCCATCTAAA CCAGACGTCA CGGATGTCAG CCGCACCTCC 
    GTGTCTCTTT CCTGGAAGCC GAATCTGAAC GCTGGAGCCA CACCCACCTC ATACGTCATT 
    GAGGCCTTCA GCCATGCAGC AGGCAGCAGC TGGCAGACGC TCGCCGATCA TGTGAAGACG 
    GAAAGCTTCG TGCTTAAGGG TCTGAAGCCA AGTGCCGTCT ACCTGTTTCT GGTGAGAGCG 
    GCCAATGCGT ACGGCCTGAG CGACCCCAGT CCCATCAGCG ACGCTGTCAA AACACAAGAC 
    GTCCCACCAA CCAGCCAGGG AGTGGAGCAT GGCCAGATCC AGAGAGAACT GGGAGATGTC 
    GTCATTCACC TTCACAATCC CACAATTCTC TCATCGTCTT CCGTCAGAGT CCAGTGGACT 
    GTGGAGCAGC AGTCTCAGTA TATTCAGGGC TATAAGGTGA TGTACCGTCC GTCTCCAGAA 
    GGCGCTCCGC AGCGAGTGGA TTGGGCCATG TTTGAGGTCG GAGCCCCGGG GGAGCACAGC 
    GCTGTCGTCA CCCAGCTCAA GAAGGGCATC ACCTATGAGT TCAAAGTGCG CCCTTTCTTC 
    AATGAGTTCC AGGGCACCGA CAGCGACATC AAAGTGGGAA AGACCCTGGA GGAGGCTCCC 
    AGCGCTGCTC CCAGAGGCGT CACCGTAACA GGAAGTGGAG ACAACGGCAC AGCAGTCCTG 
    GTCGCCTGGC AACCGCCGCC AGAGGAGGAG CAGAACGGCG TTGTGCAGGA GTACAAGATC 
    TGGTGTCTTG GTAATGAAAG CCGATACCAC ATCAACCGCA CAGTGGATGG TTCTACACAC 
    TCGGTGTTGA TTCCTGGTCT GGTGGCAGGA GTCACATACA GGCTAGAGGT GGCAGCAGGC 
    ACTGGGGCCG GACCCGGGGT CAAGAGCGAC CCTACGTCCT TCCAGCTTGA CTCTTCTGGC 
    CGTGTTCTCC CGCCGCCGCT GGACGACGAT AACACCATTT CCCAGCAGAT CTCAGACGTG 
    GTGCGTCAGC CGGCTTTCAT CGCGGGCATC GGCGCCGCCT GCTGGATCAT CCTGATGGTG 
    TTCAGCATCT GGCTATACCG CCACCGCAAG AAGAGGAACG GCCTCAGCAG CAGCTACGCC 
    GGCATCCGCA AAGTGCCCTC CTTCACCTTC ACCCCCACAG TGGCCTATCA GAGAGGCGGA 
    GAGGCCGTCA GCAGTGCAGG AAGGCCGGGT TTGTTGAATA TAGGTGAACC AGCTACACAG 
    CCCTGGCTTG CGGACACCTG GCCCAACAGC TGCTCCAATC ACAATGACTG CACTATTAAC 
    TGCTGCACCG GCAGCAATGG AAACAGCGAC AGCAACCTGG CAACTTACAG CCGCCCAGCC 
    GACTGCATCG CCAACTACAG CAACCAGATG GACAACAAGG CCACCAACCT GCTGGCTCCA 
    GATACTGGGC TTTACAGCGA CGTTGACCTT TCAAACAAGA TCAACGAGAT GAAGACCTTC 
    AACAGCCCCA ACCTGAAGGA CGGGCGTTTT ATGGGGCCTG GTGGTCAACC CACACCCTAC 
    GCCACCACTC AGCTCATTCA ATCCAGCCTG GCCAACAACA ACATGAACAG CAGCAGTGGC 
    GGAAGCGGAG GAGGAAGCGC TGACTTTAAT GAGAAGCAGA GCTGGAAGAG CAGCCAGGGA 
    ACACCTCAGA AACAGGCCGA CATCAACACA CCACTGCAGT ATAACGTCAT GGAACAAAAC 
    AAACTCAACA AGGACCACTA CCGAGGTGAG GGTCCCATCT CAGTCACCAT CCCTTATAAC 
    CAGGCCCAGG ACGTCCTCAC AGGAGAAACC TACAGCAGCT CGGACAGAGG GAGCAGCACC 
    TCTGGAAGTC AAGGGCAGAA AAAGGGTGGA CGAACAACTA AACAAGCCAA ACAGAATCCA 
    ATGAATTGGA CTGAACTGTT GCCTCCTCCT CCGGTTAATC CGCCGCCATG TCAAGAATAC 
    ACCCTGGTGA TGGACGACAG TTACGACACA GACCTGCAGT GCCCTTTGCC GCCGTCTCGG 
    ATGTATCTGC AACCTGACGA GTTGGAAGAG GAGGAGGACG AGGTGGAGCT GGAGCGTGGA 
    CCTACACCTC CTGTACGGGG AGCTGCTTCT TCACCTGCTG CCGTGTCCTA CAGCCACCAG 
    TCCACCGCAA CCCTAACCCC GTCCCCTCAG GAGGAGATGC ATCCCATGCT GCAGGACAAC 
    CCTGATCTAC ATGAGCGCAG GAGACTGTTA GTGAGTCCAC CTCCTCCACC ACGACCTCTC 
    TCTCCTCCAC ACACATACGG CTACATCTCC AGTCCCCTCG GCCTGGACAC GGACGGCCTA 
    GAGGAAGAGG ATGATGATGA AGAAGAGGGT GACGAAACGG ACGCAGAGGT AGCTCGAGTC 
    CACCAGCACC ACATGATGCA CCCTCACCAG CAGCACTTGC CCCAGCAGAG GGCTCTCCTG 
    CGGGGTCTGG AACAAACCCC GGCCTCCAGC ACCTGTGACC TGGAGAGCTC TGTGACTGGG 
    TCTATGATTA ACGGATGGGG TTCGGCTTCA GAAGAGGACA ACGCCTCGTC AGGCCGTTCC 
    AGTGCAGTCA GCTCATCGGA TGGATCGTTC TTTACCGATG CCGATTTCGC CCAGGCTGTG 
    GCAGCTGCGG CTGAATATGC TGGTCTTCGA GTGGCAAAGC ATCCTGGGAA AGGACATCAT 
    CAGCTTCCAG TGTCTGGAGC AGCACGGAAA TACCAAAACC TCCCTGCGGG ACACCGTCCA 
    GGCAGCCCAG TGTCCACAGA CAGTAACGTT AGCGCAGCCC CGGGTCAGAG GAGGCCCCTG 
    AAGAGGCAAA AACAACATCC AGCTGCTCAG ACAGGCTACC AGCGCCGAGA GGCCTACACA 
    GAGGATCTCC CCCCTCCTCC GATTCCTCCT CCAGCCATGC TGAAGTCTCC AACACACCCG 
    GCCAAAACCA TCCCCGAGGG TCCACGGGGG GCCATGTCGC CAAGATCCAG CTCTGCGGAT 
    GGCAAGGACA GGCGAACGGG TGCTGGCGGG CCCAGGGTCA GGGACGGTTC AGACACTCGG 
    ACCAGCTCTT CAGAGCGGAG GGATGGCCAA GATGTACAGA GGGGTCAGAG AACGGGCAAG 
    AATAGCAAAC AAGATGAAGC CGTGAAGAGC CGCCAGCACC CAGGCTCAGA GGACATCCTA 
    CCTTACAGTC GTCCTTCTTT CCCAGCCGTC CACAGTCCTC GAGGAGAACG GGACCCCAGT 
    TCCTCCAGTT CACTGTCCTC TCGGGGGTCT GGCGGGCGAC GGAGAGGTGA AGGGCCACCA 
    GGGGCCCGCA GAAACCCTGC AGACATTGGG CTAAACACCA TAGGAGCCTT CCAGACTGGA 
    GGGGACGAGC TTGAGATGGT GGAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214693.1
    CDS117..5246
    Translation

    Target ORF information:

    RefSeq Version XM_017359204.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359204.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCAGTTCA GAGATTGCAA TTGCAGATTT TTTATACGGA ACTGTGTCCT GCTTATGGTT 
    TGGTATCAGG GGTATTGGGG CGCCACTATG TTGCAGATGG CCCGATACAC TTTACTGGAG 
    GACTCAAAGT CTGTATTACT TCCAACAGAC ACGGGGACTC AGGATGGGAC TAGCAATAGG 
    GCCACAGAGA CCCCCACTGC AGAACACGTT GACGACAACT CTCTGGGCTA CACAGGATCC 
    CGTCTGCGGC AGGAAGATTA CGCACCCCGT ATTGTTGAAC ACCCCTCTGA TCTGATCGTA 
    TCCAAAGGCG AACCGGCCAC CCTAAACTGC AAGGCTGAAG GACGTCCCAC GCCCACAGTG 
    GAGTGGTACA AAGATGGAGA ACGAGTTGAA ACCGACCGGG ACAATCCTCG ATCCCACCGC 
    ATGCTCCTGC CCACCGGATC TCTCTTCTTT CTCCGCATTG TTCACGGCCG GCGCAGCAAA 
    CCCGACGAAG GCTCCTACGT TTGTGTGGCA CGTAACTATC TGGGAGAAGC CGTCAGCCAC 
    AACGCCTCGC TGGAAGTAGC TATTCTCCGA GATGATTTCC GACAGAACCC TGTGGACGTG 
    ATGGTGGCAG CGGGGGAGCC AGCTGTAATG GAGTGCCAGC CGCCCCGAGG ACACCCAGAG 
    CCCACCATCT CCTGGAAGAA AGATGGGGTC AATATAGACG ACAGAGACGA GAGGATAACG 
    ATACGAGGAG GAAAGCTCAT GATCACCAAC ACCAGAAAAA CGGACGCGGG GAAGTACGTG 
    TGCGTGGGAA CCAACATGAT GGGGGAACGA GAGAGCGAGA TCGCGGAGTT GACTGTTCTC 
    GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT 
    GAGTTCAGAT GTGAAGCTCA AGGAGACCCC GTGCCCACCA TCCGATGGAG GAAAGAGGAC 
    GGAGAACTGC CCAAGGGAAG GTATGAGATC TGGGAGGACC ACACTCTCAA ACTGAAGAGG 
    CTGACTTCAG GAGACGCCGG TACTTACACC TGTCAGGCGG AGAACATGAT GGGCAAGACG 
    GAGGCCAGCG CCACCCTCAC CGTCCACGTT GTGTCTGTTC CTCCAGTGTT TGTGGTCCGA 
    CCCAGGAATC AGGTGGCGGG TATGGGCCGC ACCGTCACCT TTCAGTGTGA AGCCACAGGA 
    AGTCCTCAGC CTGCAATATT CTGGCAGAAA GAAGGCAGTC AGAATCTCCT GTTCTCGTCT 
    CAGCCTCCTC CTCCGTCCAG CAGATTCTCT GTGTCTCAGA CTGGAGATCT GACCATCACA 
    GCAGTTGAGC GTTCAGATGG AGGATATTAT AGCTGTCAGG CTCTCAACAT CGCTGGCAGT 
    GTGATCACCA AAGCCCTGCT GGAGGTTACA GACGTGATTT CAGACAGGCC TCCACCAATC 
    GTCCGTCAGG GTCCAACCAA CCAGACTGTG GCAGTGGACG GGACGGTGGT TCTGAGCTGC 
    CAAGCCACCG GGACACCGAT TCCTACCATC TTATGGAGGA AAGATGGAGT TCTGGTGTCC 
    ACACATGATT CCCGACTCAA GCAGCTGGAC ACTGGAGCCC TGCAGATTCG ATACGCAAAG 
    CTGGGCGACA CTGGCATGTA CACCTGCACG GCTTCCACCC CGAGCGGAGA GGCGTCCTGG 
    AAGGCCTACC TGGAGGTGCA AGAGTTTGGT GCTCCAGTTC AACCGGGCCG TCCCACAGAT 
    CCCAACCTCA TCCCCAGCGC CCCATCTAAA CCAGACGTCA CGGATGTCAG CCGCACCTCC 
    GTGTCTCTTT CCTGGAAGCC GAATCTGAAC GCTGGAGCCA CACCCACCTC ATACGTCATT 
    GAGGCCTTCA GCCATGCAGC AGGCAGCAGC TGGCAGACGC TCGCCGATCA TGTGAAGACG 
    GAAAGCTTCG TGCTTAAGGG TCTGAAGCCA AGTGCCGTCT ACCTGTTTCT GGTGAGAGCG 
    GCCAATGCGT ACGGCCTGAG CGACCCCAGT CCCATCAGCG ACGCTGTCAA AACACAAGAC 
    GTCCCACCAA CCAGCCAGGG AGTGGAGCAT GGCCAGATCC AGAGAGAACT GGGAGATGTC 
    GTCATTCACC TTCACAATCC CACAATTCTC TCATCGTCTT CCGTCAGAGT CCAGTGGACT 
    GTGGAGCAGC AGTCTCAGTA TATTCAGGGC TATAAGGTGA TGTACCGTCC GTCTCCAGAA 
    GGCGCTCCGC AGCGAGTGGA TTGGGCCATG TTTGAGGTCG GAGCCCCGGG GGAGCACAGC 
    GCTGTCGTCA CCCAGCTCAA GAAGGGCATC ACCTATGAGT TCAAAGTGCG CCCTTTCTTC 
    AATGAGTTCC AGGGCACCGA CAGCGACATC AAAGTGGGAA AGACCCTGGA GGAGGCTCCC 
    AGCGCTGCTC CCAGAGGCGT CACCGTAACA GGAAGTGGAG ACAACGGCAC AGCAGTCCTG 
    GTCGCCTGGC AACCGCCGCC AGAGGAGGAG CAGAACGGCG TTGTGCAGGA GTACAAGATC 
    TGGTGTCTTG GTAATGAAAG CCGATACCAC ATCAACCGCA CAGTGGATGG TTCTACACAC 
    TCGGTGTTGA TTCCTGGTCT GGTGGCAGGA GTCACATACA GGCTAGAGGT GGCAGCAGGC 
    ACTGGGGCCG GACCCGGGGT CAAGAGCGAC CCTACGTCCT TCCAGCTTGA CTCTTCTGGC 
    CGTGTTCTCC CGCCGCCGCT GGACGACGAT AACACCATTT CCCAGCAGAT CTCAGACGTG 
    GTGCGTCAGC CGGCTTTCAT CGCGGGCATC GGCGCCGCCT GCTGGATCAT CCTGATGGTG 
    TTCAGCATCT GGCTATACCG CCACCGCAAG AAGAGGAACG GCCTCAGCAG CAGCTACGCC 
    GGCATCCGCA AAGTGCCCTC CTTCACCTTC ACCCCCACAG TGGCCTATCA GAGAGGCGGA 
    GAGGCCGTCA GCAGTGCAGG AAGGCCGGGT TTGTTGAATA TAGGTGAACC AGCTACACAG 
    CCCTGGCTTG CGGACACCTG GCCCAACAGC TGCTCCAATC ACAATGACTG CACTATTAAC 
    TGCTGCACCG GCAGCAATGG AAACAGCGAC AGCAACCTGG CAACTTACAG CCGCCCAGCC 
    GACTGCATCG CCAACTACAG CAACCAGATG GACAACAAGG CCACCAACCT GCTGGCTCCA 
    GATACTGGGC TTTACAGCGA CGTTGACCTT TCAAACAAGA TCAACGAGAT GAAGACCTTC 
    AACAGCCCCA ACCTGAAGGA CGGGCGTTTT ATGGGGCCTG GTGGTCAACC CACACCCTAC 
    GCCACCACTC AGCTCATTCA ATCCAGCCTG GCCAACAACA ACATGAACAG CAGCAGTGGC 
    GGAAGCGGAG GAGGAAGCGC TGACTTTAAT GAGAAGCAGA GCTGGAAGAG CAGCCAGGGA 
    ACACCTCAGA AACAGGCCGA CATCAACACA CCACTGCAGT ATAACGTCAT GGAACAAAAC 
    AAACTCAACA AGGACCACTA CCGAGGTGAG GGTCCCATCT CAGTCACCAT CCCTTATAAC 
    CAGGCCCAGG ACGTCCTCAC AGGAGAAACC TACAGCAGCT CGGACAGAGG GAGCAGCACC 
    TCTGGAAGTC AAGGGCAGAA AAAGGGTGGA CGAACAACTA AACAAGCCAA ACAGAATCCA 
    ATGAATTGGA CTGAACTGTT GCCTCCTCCT CCGGTTAATC CGCCGCCATG TCAAGAATAC 
    ACCCTGGTGA TGGACGACAG TTACGACACA GACCTGCAGT GCCCTTTGCC GCCGTCTCGG 
    ATGTATCTGC AACCTGACGA GTTGGAAGAG GAGGAGGACG AGGTGGAGCT GGAGCGTGGA 
    CCTACACCTC CTGTACGGGG AGCTGCTTCT TCACCTGCTG CCGTGTCCTA CAGCCACCAG 
    TCCACCGCAA CCCTAACCCC GTCCCCTCAG GAGGAGATGC ATCCCATGCT GCAGGACAAC 
    CCTGATCTAC ATGAGCGCAG GAGACTGTTA GTGAGTCCAC CTCCTCCACC ACGACCTCTC 
    TCTCCTCCAC ACACATACGG CTACATCTCC AGTCCCCTCG GCCTGGACAC GGACGGCCTA 
    GAGGAAGAGG ATGATGATGA AGAAGAGGGT GACGAAACGG ACGCAGAGGT AGCTCGAGTC 
    CACCAGCACC ACATGATGCA CCCTCACCAG CAGCACTTGC CCCAGCAGAG GGCTCTCCTG 
    CGGGGTCTGG AACAAACCCC GGCCTCCAGC ACCTGTGACC TGGAGAGCTC TGTGACTGGG 
    TCTATGATTA ACGGATGGGG TTCGGCTTCA GAAGAGGACA ACGCCTCGTC AGGCCGTTCC 
    AGTGCAGTCA GCTCATCGGA TGGATCGTTC TTTACCGATG CCGATTTCGC CCAGGCTGTG 
    GCAGCTGCGG CTGAATATGC TGGTCTTCGA GTGGCAAAGC ATCCTGGGAA AGGACATCAT 
    CAGCTTCCAG TGTCTGGAGC AGCACGGAAA TACCAAAACC TCCCTGCGGG ACACCGTCCA 
    GGCAGCCCAG TGTCCACAGA CAGTAACGTT AGCGCAGCCC CGGGTCAGAG GAGGCCCCTG 
    AAGAGGCAAA AACAACATCC AGCTGCTCAG ACAGGCTACC AGCGCCGAGA GGCCTACACA 
    GAGGATCTCC CCCCTCCTCC GATTCCTCCT CCAGCCATGC TGAAGTCTCC AACACACCCG 
    GCCAAAACCA TCCCCGAGGG TCCACGGGGG GCCATGTCGC CAAGATCCAG CTCTGCGGAT 
    GGCAAGGACA GGCGAACGGG TGCTGGCGGG CCCAGGGTCA GGGACGGTTC AGACACTCGG 
    ACCAGCTCTT CAGAGCGGAG GGATGGCCAA GATGTACAGA GGGGTCAGAG AACGGGCAAG 
    AATAGCAAAC AAGATGAAGC CGTGAAGAGC CGCCAGCACC CAGGCTCAGA GGACATCCTA 
    CCTTACAGTC GTCCTTCTTT CCCAGCCGTC CACAGTCCTC GAGGAGAACG GGACCCCAGT 
    TCCTCCAGTT CACTGTCCTC TCGGGGGTCT GGCGGGCGAC GGAGAGGTGA AGGGCCACCA 
    GGGGCCCGCA GAAACCCTGC AGACATTGGG CTAAACACCA TAGGAGCCTT CCAGACTGGA 
    GGGGACGAGC TTGAGATGGT GGAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359205.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214694.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa55056
    Clone ID Related Accession (Same CDS sequence) XM_017359205.1
    Accession Version XM_017359205.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCAGTTCA GAGATTGCAA TTGCAGATTT TTTATACGGA ACTGTGTCCT GCTTATGGTT 
    TGGTATCAGG GGTATTGGGG CGCCACTATG TTGCAGATGG CCCGATACAC TTTACTGGAG 
    GACTCAAAGT CTGTATTACT TCCAACAGAC ACGGGGACTC AGGATGGGAC TAGCAATAGG 
    GCCACAGAGA CCCCCACTGC AGAACACGTT GACGACAACT CTCTGGGCTA CACAGGATCC 
    CGTCTGCGGC AGGAAGATTA CGCACCCCGT ATTGTTGAAC ACCCCTCTGA TCTGATCGTA 
    TCCAAAGGCG AACCGGCCAC CCTAAACTGC AAGGCTGAAG GACGTCCCAC GCCCACAGTG 
    GAGTGGTACA AAGATGGAGA ACGAGTTGAA ACCGACCGGG ACAATCCTCG ATCCCACCGC 
    ATGCTCCTGC CCACCGGATC TCTCTTCTTT CTCCGCATTG TTCACGGCCG GCGCAGCAAA 
    CCCGACGAAG GCTCCTACGT TTGTGTGGCA CGTAACTATC TGGGAGAAGC CGTCAGCCAC 
    AACGCCTCGC TGGAAGTAGC TATTCTCCGA GATGATTTCC GACAGAACCC TGTGGACGTG 
    ATGGTGGCAG CGGGGGAGCC AGCTGTAATG GAGTGCCAGC CGCCCCGAGG ACACCCAGAG 
    CCCACCATCT CCTGGAAGAA AGATGGGGTC AATATAGACG ACAGAGACGA GAGGATAACG 
    ATACGAGGAG GAAAGCTCAT GATCACCAAC ACCAGAAAAA CGGACGCGGG GAAGTACGTG 
    TGCGTGGGAA CCAACATGAT GGGGGAACGA GAGAGCGAGA TCGCGGAGTT GACTGTTCTC 
    GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT 
    GAGTTCAGAT GTGAAGCTCA AGGAGACCCC GTGCCCACCA TCCGATGGAG GAAAGAGGAC 
    GGAGAACTGC CCAAGGGAAG GTATGAGATC TGGGAGGACC ACACTCTCAA ACTGAAGAGG 
    CTGACTTCAG GAGACGCCGG TACTTACACC TGTCAGGCGG AGAACATGAT GGGCAAGACG 
    GAGGCCAGCG CCACCCTCAC CGTCCACGTT GTGTCTGTTC CTCCAGTGTT TGTGGTCCGA 
    CCCAGGAATC AGGTGGCGGG TATGGGCCGC ACCGTCACCT TTCAGTGTGA AGCCACAGGA 
    AGTCCTCAGC CTGCAATATT CTGGCAGAAA GAAGGCAGTC AGAATCTCCT GTTCTCGTCT 
    CAGCCTCCTC CTCCGTCCAG CAGATTCTCT GTGTCTCAGA CTGGAGATCT GACCATCACA 
    GCAGTTGAGC GTTCAGATGG AGGATATTAT AGCTGTCAGG CTCTCAACAT CGCTGGCAGT 
    GTGATCACCA AAGCCCTGCT GGAGGTTACA GACGTGATTT CAGACAGGCC TCCACCAATC 
    GTCCGTCAGG GTCCAACCAA CCAGACTGTG GCAGTGGACG GGACGGTGGT TCTGAGCTGC 
    CAAGCCACCG GGACACCGAT TCCTACCATC TTATGGAGGA AAGATGGAGT TCTGGTGTCC 
    ACACATGATT CCCGACTCAA GCAGCTGGAC ACTGGAGCCC TGCAGATTCG ATACGCAAAG 
    CTGGGCGACA CTGGCATGTA CACCTGCACG GCTTCCACCC CGAGCGGAGA GGCGTCCTGG 
    AAGGCCTACC TGGAGGTGCA AGAGTTTGGT GCTCCAGTTC AACCGGGCCG TCCCACAGAT 
    CCCAACCTCA TCCCCAGCGC CCCATCTAAA CCAGACGTCA CGGATGTCAG CCGCACCTCC 
    GTGTCTCTTT CCTGGAAGCC GAATCTGAAC GCTGGAGCCA CACCCACCTC ATACGTCATT 
    GAGGCCTTCA GCCATGCAGC AGGCAGCAGC TGGCAGACGC TCGCCGATCA TGTGAAGACG 
    GAAAGCTTCG TGCTTAAGGG TCTGAAGCCA AGTGCCGTCT ACCTGTTTCT GGTGAGAGCG 
    GCCAATGCGT ACGGCCTGAG CGACCCCAGT CCCATCAGCG ACGCTGTCAA AACACAAGAC 
    GTCCCACCAA CCAGCCAGGG AGTGGAGCAT GGCCAGATCC AGAGAGAACT GGGAGATGTC 
    GTCATTCACC TTCACAATCC CACAATTCTC TCATCGTCTT CCGTCAGAGT CCAGTGGACT 
    GTGGAGCAGC AGTCTCAGTA TATTCAGGGC TATAAGGTGA TGTACCGTCC GTCTCCAGAA 
    GGCGCTCCGC AGCGAGTGGA TTGGGCCATG TTTGAGGTCG GAGCCCCGGG GGAGCACAGC 
    GCTGTCGTCA CCCAGCTCAA GAAGGGCATC ACCTATGAGT TCAAAGTGCG CCCTTTCTTC 
    AATGAGTTCC AGGGCACCGA CAGCGACATC AAAGTGGGAA AGACCCTGGA GGAGGCTCCC 
    AGCGCTGCTC CCAGAGGCGT CACCGTAACA GGAAGTGGAG ACAACGGCAC AGCAGTCCTG 
    GTCGCCTGGC AACCGCCGCC AGAGGAGGAG CAGAACGGCG TTGTGCAGGA GTACAAGATC 
    TGGTGTCTTG GTAATGAAAG CCGATACCAC ATCAACCGCA CAGTGGATGG TTCTACACAC 
    TCGGTGTTGA TTCCTGGTCT GGTGGCAGGA GTCACATACA GGCTAGAGGT GGCAGCAGGC 
    ACTGGGGCCG GACCCGGGGT CAAGAGCGAC CCTACGTCCT TCCAGCTTGA CTCTTCTGGC 
    CGTGTTCTCC CGCCGCCGCT GGACGACGAT AACACCATTT CCCAGCAGAT CTCAGACGTG 
    GTGCGTCAGC CGGCTTTCAT CGCGGGCATC GGCGCCGCCT GCTGGATCAT CCTGATGGTG 
    TTCAGCATCT GGCTATACCG CCACCGCAAG AAGAGGAACG GCCTCAGCAG CAGCTACGCC 
    GGCATCCGCA AAGTGCCCTC CTTCACCTTC ACCCCCACAG TGGCCTATCA GAGAGGCGGA 
    GAGGCCGTCA GCAGTGCAGG AAGGCCGGGT TTGTTGAATA TAGGTGAACC AGCTACACAG 
    CCCTGGCTTG CGGACACCTG GCCCAACAGC TGCTCCAATC ACAATGACTG CACTATTAAC 
    TGCTGCACCG GCAGCAATGG AAACAGCGAC AGCAACCTGG CAACTTACAG CCGCCCAGCC 
    GACTGCATCG CCAACTACAG CAACCAGATG GACAACAAGG CCACCAACCT GCTGGCTCCA 
    GATACTGGGC TTTACAGCGA CGTTGACCTT TCAAACAAGA TCAACGAGAT GAAGACCTTC 
    AACAGCCCCA ACCTGAAGGA CGGGCGTTTT ATGGGGCCTG GTGGTCAACC CACACCCTAC 
    GCCACCACTC AGCTCATTCA ATCCAGCCTG GCCAACAACA ACATGAACAG CAGCAGTGGC 
    GGAAGCGGAG GAGGAAGCGC TGACTTTAAT GAGAAGCAGA GCTGGAAGAG CAGCCAGGGA 
    ACACCTCAGA AACAGGCCGA CATCAACACA CCACTGCAGT ATAACGTCAT GGAACAAAAC 
    AAACTCAACA AGGACCACTA CCGAGGTGAG GGTCCCATCT CAGTCACCAT CCCTTATAAC 
    CAGGCCCAGG ACGTCCTCAC AGGAGAAACC TACAGCAGCT CGGACAGAGG GAGCAGCACC 
    TCTGGAAGTC AAGGGCAGAA AAAGGGTGGA CGAACAACTA AACAAGCCAA ACAGAATCCA 
    ATGAATTGGA CTGAACTGTT GCCTCCTCCT CCGGTTAATC CGCCGCCATG TCAAGAATAC 
    ACCCTGGTGA TGGACGACAG TTACGACACA GACCTGCAGT GCCCTTTGCC GCCGTCTCGG 
    ATGTATCTGC AACCTGACGA GTTGGAAGAG GAGGAGGACG AGGTGGAGCT GGAGCGTGGA 
    CCTACACCTC CTGTACGGGG AGCTGCTTCT TCACCTGCTG CCGTGTCCTA CAGCCACCAG 
    TCCACCGCAA CCCTAACCCC GTCCCCTCAG GAGGAGATGC ATCCCATGCT GCAGGACAAC 
    CCTGATCTAC ATGAGCGCAG GAGACTGTTA GTGAGTCCAC CTCCTCCACC ACGACCTCTC 
    TCTCCTCCAC ACACATACGG CTACATCTCC AGTCCCCTCG GCCTGGACAC GGACGGCCTA 
    GAGGAAGAGG ATGATGATGA AGAAGAGGGT GACGAAACGG ACGCAGAGGT AGCTCGAGTC 
    CACCAGCACC ACATGATGCA CCCTCACCAG CAGCACTTGC CCCAGCAGAG GGCTCTCCTG 
    CGGGGTCTGG AACAAACCCC GGCCTCCAGC ACCTGTGACC TGGAGAGCTC TGTGACTGGG 
    TCTATGATTA ACGGATGGGG TTCGGCTTCA GAAGAGGACA ACGCCTCGTC AGGCCGTTCC 
    AGTGCAGTCA GCTCATCGGA TGGATCGTTC TTTACCGATG CCGATTTCGC CCAGGCTGTG 
    GCAGCTGCGG CTGAATATGC TGGTCTTCGA GTGGCAAAGC ATCCTGGGAA AGGACATCAT 
    CAGCTTCCAG TGTCTGGAGC ACGGAAATAC CAAAACCTCC CTGCGGGACA CCGTCCAGGC 
    AGCCCAGTGT CCACAGACAG TAACGTTAGC GCAGCCCCGG GTCAGAGGAG GCCCCTGAAG 
    AGGCAAAAAC AACATCCAGC TGCTCAGACA GGCTACCAGC GCCGAGAGGC CTACACAGAG 
    GATCTCCCCC CTCCTCCGAT TCCTCCTCCA GCCATGCTGA AGTCTCCAAC ACACCCGGCC 
    AAAACCATCC CCGAGGGTCC ACGGGGGGCC ATGTCGCCAA GATCCAGCTC TGCGGATGGC 
    AAGGACAGGC GAACGGGTGC TGGCGGGCCC AGGGTCAGGG ACGGTTCAGA CACTCGGACC 
    AGCTCTTCAG AGCGGAGGGA TGGCCAAGAT GTACAGAGGG GTCAGAGAAC GGGCAAGAAT 
    AGCAAACAAG ATGAAGCCGT GAAGAGCCGC CAGCACCCAG GCTCAGAGGA CATCCTACCT 
    TACAGTCGTC CTTCTTTCCC AGCCGTCCAC AGTCCTCGAG GAGAACGGGA CCCCAGTTCC 
    TCCAGTTCAC TGTCCTCTCG GGGGTCTGGC GGGCGACGGA GAGGTGAAGG GCCACCAGGG 
    GCCCGCAGAA ACCCTGCAGA CATTGGGCTA AACACCATAG GAGCCTTCCA GACTGGAGGG 
    GACGAGCTTG AGATGGTGGA GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214694.1
    CDS116..5242
    Translation

    Target ORF information:

    RefSeq Version XM_017359205.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359205.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCAGTTCA GAGATTGCAA TTGCAGATTT TTTATACGGA ACTGTGTCCT GCTTATGGTT 
    TGGTATCAGG GGTATTGGGG CGCCACTATG TTGCAGATGG CCCGATACAC TTTACTGGAG 
    GACTCAAAGT CTGTATTACT TCCAACAGAC ACGGGGACTC AGGATGGGAC TAGCAATAGG 
    GCCACAGAGA CCCCCACTGC AGAACACGTT GACGACAACT CTCTGGGCTA CACAGGATCC 
    CGTCTGCGGC AGGAAGATTA CGCACCCCGT ATTGTTGAAC ACCCCTCTGA TCTGATCGTA 
    TCCAAAGGCG AACCGGCCAC CCTAAACTGC AAGGCTGAAG GACGTCCCAC GCCCACAGTG 
    GAGTGGTACA AAGATGGAGA ACGAGTTGAA ACCGACCGGG ACAATCCTCG ATCCCACCGC 
    ATGCTCCTGC CCACCGGATC TCTCTTCTTT CTCCGCATTG TTCACGGCCG GCGCAGCAAA 
    CCCGACGAAG GCTCCTACGT TTGTGTGGCA CGTAACTATC TGGGAGAAGC CGTCAGCCAC 
    AACGCCTCGC TGGAAGTAGC TATTCTCCGA GATGATTTCC GACAGAACCC TGTGGACGTG 
    ATGGTGGCAG CGGGGGAGCC AGCTGTAATG GAGTGCCAGC CGCCCCGAGG ACACCCAGAG 
    CCCACCATCT CCTGGAAGAA AGATGGGGTC AATATAGACG ACAGAGACGA GAGGATAACG 
    ATACGAGGAG GAAAGCTCAT GATCACCAAC ACCAGAAAAA CGGACGCGGG GAAGTACGTG 
    TGCGTGGGAA CCAACATGAT GGGGGAACGA GAGAGCGAGA TCGCGGAGTT GACTGTTCTC 
    GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT 
    GAGTTCAGAT GTGAAGCTCA AGGAGACCCC GTGCCCACCA TCCGATGGAG GAAAGAGGAC 
    GGAGAACTGC CCAAGGGAAG GTATGAGATC TGGGAGGACC ACACTCTCAA ACTGAAGAGG 
    CTGACTTCAG GAGACGCCGG TACTTACACC TGTCAGGCGG AGAACATGAT GGGCAAGACG 
    GAGGCCAGCG CCACCCTCAC CGTCCACGTT GTGTCTGTTC CTCCAGTGTT TGTGGTCCGA 
    CCCAGGAATC AGGTGGCGGG TATGGGCCGC ACCGTCACCT TTCAGTGTGA AGCCACAGGA 
    AGTCCTCAGC CTGCAATATT CTGGCAGAAA GAAGGCAGTC AGAATCTCCT GTTCTCGTCT 
    CAGCCTCCTC CTCCGTCCAG CAGATTCTCT GTGTCTCAGA CTGGAGATCT GACCATCACA 
    GCAGTTGAGC GTTCAGATGG AGGATATTAT AGCTGTCAGG CTCTCAACAT CGCTGGCAGT 
    GTGATCACCA AAGCCCTGCT GGAGGTTACA GACGTGATTT CAGACAGGCC TCCACCAATC 
    GTCCGTCAGG GTCCAACCAA CCAGACTGTG GCAGTGGACG GGACGGTGGT TCTGAGCTGC 
    CAAGCCACCG GGACACCGAT TCCTACCATC TTATGGAGGA AAGATGGAGT TCTGGTGTCC 
    ACACATGATT CCCGACTCAA GCAGCTGGAC ACTGGAGCCC TGCAGATTCG ATACGCAAAG 
    CTGGGCGACA CTGGCATGTA CACCTGCACG GCTTCCACCC CGAGCGGAGA GGCGTCCTGG 
    AAGGCCTACC TGGAGGTGCA AGAGTTTGGT GCTCCAGTTC AACCGGGCCG TCCCACAGAT 
    CCCAACCTCA TCCCCAGCGC CCCATCTAAA CCAGACGTCA CGGATGTCAG CCGCACCTCC 
    GTGTCTCTTT CCTGGAAGCC GAATCTGAAC GCTGGAGCCA CACCCACCTC ATACGTCATT 
    GAGGCCTTCA GCCATGCAGC AGGCAGCAGC TGGCAGACGC TCGCCGATCA TGTGAAGACG 
    GAAAGCTTCG TGCTTAAGGG TCTGAAGCCA AGTGCCGTCT ACCTGTTTCT GGTGAGAGCG 
    GCCAATGCGT ACGGCCTGAG CGACCCCAGT CCCATCAGCG ACGCTGTCAA AACACAAGAC 
    GTCCCACCAA CCAGCCAGGG AGTGGAGCAT GGCCAGATCC AGAGAGAACT GGGAGATGTC 
    GTCATTCACC TTCACAATCC CACAATTCTC TCATCGTCTT CCGTCAGAGT CCAGTGGACT 
    GTGGAGCAGC AGTCTCAGTA TATTCAGGGC TATAAGGTGA TGTACCGTCC GTCTCCAGAA 
    GGCGCTCCGC AGCGAGTGGA TTGGGCCATG TTTGAGGTCG GAGCCCCGGG GGAGCACAGC 
    GCTGTCGTCA CCCAGCTCAA GAAGGGCATC ACCTATGAGT TCAAAGTGCG CCCTTTCTTC 
    AATGAGTTCC AGGGCACCGA CAGCGACATC AAAGTGGGAA AGACCCTGGA GGAGGCTCCC 
    AGCGCTGCTC CCAGAGGCGT CACCGTAACA GGAAGTGGAG ACAACGGCAC AGCAGTCCTG 
    GTCGCCTGGC AACCGCCGCC AGAGGAGGAG CAGAACGGCG TTGTGCAGGA GTACAAGATC 
    TGGTGTCTTG GTAATGAAAG CCGATACCAC ATCAACCGCA CAGTGGATGG TTCTACACAC 
    TCGGTGTTGA TTCCTGGTCT GGTGGCAGGA GTCACATACA GGCTAGAGGT GGCAGCAGGC 
    ACTGGGGCCG GACCCGGGGT CAAGAGCGAC CCTACGTCCT TCCAGCTTGA CTCTTCTGGC 
    CGTGTTCTCC CGCCGCCGCT GGACGACGAT AACACCATTT CCCAGCAGAT CTCAGACGTG 
    GTGCGTCAGC CGGCTTTCAT CGCGGGCATC GGCGCCGCCT GCTGGATCAT CCTGATGGTG 
    TTCAGCATCT GGCTATACCG CCACCGCAAG AAGAGGAACG GCCTCAGCAG CAGCTACGCC 
    GGCATCCGCA AAGTGCCCTC CTTCACCTTC ACCCCCACAG TGGCCTATCA GAGAGGCGGA 
    GAGGCCGTCA GCAGTGCAGG AAGGCCGGGT TTGTTGAATA TAGGTGAACC AGCTACACAG 
    CCCTGGCTTG CGGACACCTG GCCCAACAGC TGCTCCAATC ACAATGACTG CACTATTAAC 
    TGCTGCACCG GCAGCAATGG AAACAGCGAC AGCAACCTGG CAACTTACAG CCGCCCAGCC 
    GACTGCATCG CCAACTACAG CAACCAGATG GACAACAAGG CCACCAACCT GCTGGCTCCA 
    GATACTGGGC TTTACAGCGA CGTTGACCTT TCAAACAAGA TCAACGAGAT GAAGACCTTC 
    AACAGCCCCA ACCTGAAGGA CGGGCGTTTT ATGGGGCCTG GTGGTCAACC CACACCCTAC 
    GCCACCACTC AGCTCATTCA ATCCAGCCTG GCCAACAACA ACATGAACAG CAGCAGTGGC 
    GGAAGCGGAG GAGGAAGCGC TGACTTTAAT GAGAAGCAGA GCTGGAAGAG CAGCCAGGGA 
    ACACCTCAGA AACAGGCCGA CATCAACACA CCACTGCAGT ATAACGTCAT GGAACAAAAC 
    AAACTCAACA AGGACCACTA CCGAGGTGAG GGTCCCATCT CAGTCACCAT CCCTTATAAC 
    CAGGCCCAGG ACGTCCTCAC AGGAGAAACC TACAGCAGCT CGGACAGAGG GAGCAGCACC 
    TCTGGAAGTC AAGGGCAGAA AAAGGGTGGA CGAACAACTA AACAAGCCAA ACAGAATCCA 
    ATGAATTGGA CTGAACTGTT GCCTCCTCCT CCGGTTAATC CGCCGCCATG TCAAGAATAC 
    ACCCTGGTGA TGGACGACAG TTACGACACA GACCTGCAGT GCCCTTTGCC GCCGTCTCGG 
    ATGTATCTGC AACCTGACGA GTTGGAAGAG GAGGAGGACG AGGTGGAGCT GGAGCGTGGA 
    CCTACACCTC CTGTACGGGG AGCTGCTTCT TCACCTGCTG CCGTGTCCTA CAGCCACCAG 
    TCCACCGCAA CCCTAACCCC GTCCCCTCAG GAGGAGATGC ATCCCATGCT GCAGGACAAC 
    CCTGATCTAC ATGAGCGCAG GAGACTGTTA GTGAGTCCAC CTCCTCCACC ACGACCTCTC 
    TCTCCTCCAC ACACATACGG CTACATCTCC AGTCCCCTCG GCCTGGACAC GGACGGCCTA 
    GAGGAAGAGG ATGATGATGA AGAAGAGGGT GACGAAACGG ACGCAGAGGT AGCTCGAGTC 
    CACCAGCACC ACATGATGCA CCCTCACCAG CAGCACTTGC CCCAGCAGAG GGCTCTCCTG 
    CGGGGTCTGG AACAAACCCC GGCCTCCAGC ACCTGTGACC TGGAGAGCTC TGTGACTGGG 
    TCTATGATTA ACGGATGGGG TTCGGCTTCA GAAGAGGACA ACGCCTCGTC AGGCCGTTCC 
    AGTGCAGTCA GCTCATCGGA TGGATCGTTC TTTACCGATG CCGATTTCGC CCAGGCTGTG 
    GCAGCTGCGG CTGAATATGC TGGTCTTCGA GTGGCAAAGC ATCCTGGGAA AGGACATCAT 
    CAGCTTCCAG TGTCTGGAGC ACGGAAATAC CAAAACCTCC CTGCGGGACA CCGTCCAGGC 
    AGCCCAGTGT CCACAGACAG TAACGTTAGC GCAGCCCCGG GTCAGAGGAG GCCCCTGAAG 
    AGGCAAAAAC AACATCCAGC TGCTCAGACA GGCTACCAGC GCCGAGAGGC CTACACAGAG 
    GATCTCCCCC CTCCTCCGAT TCCTCCTCCA GCCATGCTGA AGTCTCCAAC ACACCCGGCC 
    AAAACCATCC CCGAGGGTCC ACGGGGGGCC ATGTCGCCAA GATCCAGCTC TGCGGATGGC 
    AAGGACAGGC GAACGGGTGC TGGCGGGCCC AGGGTCAGGG ACGGTTCAGA CACTCGGACC 
    AGCTCTTCAG AGCGGAGGGA TGGCCAAGAT GTACAGAGGG GTCAGAGAAC GGGCAAGAAT 
    AGCAAACAAG ATGAAGCCGT GAAGAGCCGC CAGCACCCAG GCTCAGAGGA CATCCTACCT 
    TACAGTCGTC CTTCTTTCCC AGCCGTCCAC AGTCCTCGAG GAGAACGGGA CCCCAGTTCC 
    TCCAGTTCAC TGTCCTCTCG GGGGTCTGGC GGGCGACGGA GAGGTGAAGG GCCACCAGGG 
    GCCCGCAGAA ACCCTGCAGA CATTGGGCTA AACACCATAG GAGCCTTCCA GACTGGAGGG 
    GACGAGCTTG AGATGGTGGA GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359206.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214695.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa55057
    Clone ID Related Accession (Same CDS sequence) XM_017359206.1
    Accession Version XM_017359206.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5121bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCAGTTCA GAGATTGCAA TTGCAGATTT TTTATACGGA ACTGTGTCCT GCTTATGGTT 
    TGGTATCAGG GGTATTGGGG CGCCACTATG TTGCAGATGG CCCGATACAC TTTACTGGAG 
    GACTCAAAGT CTGTATTACT TCCAACAGAC ACGGGGACTC AGGATGGGAC TAGCAATAGG 
    GCCACAGAGA CCCCCACTGC AGAACACGTT GACGACAACT CTCTGGGCTA CACAGGATCC 
    CGTCTGCGGC AGGAAGATTA CGCACCCCGT ATTGTTGAAC ACCCCTCTGA TCTGATCGTA 
    TCCAAAGGCG AACCGGCCAC CCTAAACTGC AAGGCTGAAG GACGTCCCAC GCCCACAGTG 
    GAGTGGTACA AAGATGGAGA ACGAGTTGAA ACCGACCGGG ACAATCCTCG ATCCCACCGC 
    ATGCTCCTGC CCACCGGATC TCTCTTCTTT CTCCGCATTG TTCACGGCCG GCGCAGCAAA 
    CCCGACGAAG GCTCCTACGT TTGTGTGGCA CGTAACTATC TGGGAGAAGC CGTCAGCCAC 
    AACGCCTCGC TGGAAGTAGC TATTCTCCGA GATGATTTCC GACAGAACCC TGTGGACGTG 
    ATGGTGGCAG CGGGGGAGCC AGCTGTAATG GAGTGCCAGC CGCCCCGAGG ACACCCAGAG 
    CCCACCATCT CCTGGAAGAA AGATGGGGTC AATATAGACG ACAGAGACGA GAGGATAACG 
    ATACGAGGAG GAAAGCTCAT GATCACCAAC ACCAGAAAAA CGGACGCGGG GAAGTACGTG 
    TGCGTGGGAA CCAACATGAT GGGGGAACGA GAGAGCGAGA TCGCGGAGTT GACTGTTCTC 
    GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT 
    GAGTTCAGAT GTGAAGCTCA AGGAGACCCC GTGCCCACCA TCCGATGGAG GAAAGAGGAC 
    GGAGAACTGC CCAAGGGAAG GTATGAGATC TGGGAGGACC ACACTCTCAA ACTGAAGAGG 
    CTGACTTCAG GAGACGCCGG TACTTACACC TGTCAGGCGG AGAACATGAT GGGCAAGACG 
    GAGGCCAGCG CCACCCTCAC CGTCCACGTT CCTCCAGTGT TTGTGGTCCG ACCCAGGAAT 
    CAGGTGGCGG GTATGGGCCG CACCGTCACC TTTCAGTGTG AAGCCACAGG AAGTCCTCAG 
    CCTGCAATAT TCTGGCAGAA AGAAGGCAGT CAGAATCTCC TGTTCTCGTC TCAGCCTCCT 
    CCTCCGTCCA GCAGATTCTC TGTGTCTCAG ACTGGAGATC TGACCATCAC AGCAGTTGAG 
    CGTTCAGATG GAGGATATTA TAGCTGTCAG GCTCTCAACA TCGCTGGCAG TGTGATCACC 
    AAAGCCCTGC TGGAGGTTAC AGACGTGATT TCAGACAGGC CTCCACCAAT CGTCCGTCAG 
    GGTCCAACCA ACCAGACTGT GGCAGTGGAC GGGACGGTGG TTCTGAGCTG CCAAGCCACC 
    GGGACACCGA TTCCTACCAT CTTATGGAGG AAAGATGGAG TTCTGGTGTC CACACATGAT 
    TCCCGACTCA AGCAGCTGGA CACTGGAGCC CTGCAGATTC GATACGCAAA GCTGGGCGAC 
    ACTGGCATGT ACACCTGCAC GGCTTCCACC CCGAGCGGAG AGGCGTCCTG GAAGGCCTAC 
    CTGGAGGTGC AAGAGTTTGG TGCTCCAGTT CAACCGGGCC GTCCCACAGA TCCCAACCTC 
    ATCCCCAGCG CCCCATCTAA ACCAGACGTC ACGGATGTCA GCCGCACCTC CGTGTCTCTT 
    TCCTGGAAGC CGAATCTGAA CGCTGGAGCC ACACCCACCT CATACGTCAT TGAGGCCTTC 
    AGCCATGCAG CAGGCAGCAG CTGGCAGACG CTCGCCGATC ATGTGAAGAC GGAAAGCTTC 
    GTGCTTAAGG GTCTGAAGCC AAGTGCCGTC TACCTGTTTC TGGTGAGAGC GGCCAATGCG 
    TACGGCCTGA GCGACCCCAG TCCCATCAGC GACGCTGTCA AAACACAAGA CGTCCCACCA 
    ACCAGCCAGG GAGTGGAGCA TGGCCAGATC CAGAGAGAAC TGGGAGATGT CGTCATTCAC 
    CTTCACAATC CCACAATTCT CTCATCGTCT TCCGTCAGAG TCCAGTGGAC TGTGGAGCAG 
    CAGTCTCAGT ATATTCAGGG CTATAAGGTG ATGTACCGTC CGTCTCCAGA AGGCGCTCCG 
    CAGCGAGTGG ATTGGGCCAT GTTTGAGGTC GGAGCCCCGG GGGAGCACAG CGCTGTCGTC 
    ACCCAGCTCA AGAAGGGCAT CACCTATGAG TTCAAAGTGC GCCCTTTCTT CAATGAGTTC 
    CAGGGCACCG ACAGCGACAT CAAAGTGGGA AAGACCCTGG AGGAGGCTCC CAGCGCTGCT 
    CCCAGAGGCG TCACCGTAAC AGGAAGTGGA GACAACGGCA CAGCAGTCCT GGTCGCCTGG 
    CAACCGCCGC CAGAGGAGGA GCAGAACGGC GTTGTGCAGG AGTACAAGAT CTGGTGTCTT 
    GGTAATGAAA GCCGATACCA CATCAACCGC ACAGTGGATG GTTCTACACA CTCGGTGTTG 
    ATTCCTGGTC TGGTGGCAGG AGTCACATAC AGGCTAGAGG TGGCAGCAGG CACTGGGGCC 
    GGACCCGGGG TCAAGAGCGA CCCTACGTCC TTCCAGCTTG ACTCTTCTGG CCGTGTTCTC 
    CCGCCGCCGC TGGACGACGA TAACACCATT TCCCAGCAGA TCTCAGACGT GGTGCGTCAG 
    CCGGCTTTCA TCGCGGGCAT CGGCGCCGCC TGCTGGATCA TCCTGATGGT GTTCAGCATC 
    TGGCTATACC GCCACCGCAA GAAGAGGAAC GGCCTCAGCA GCAGCTACGC CGGCATCCGC 
    AAAGTGCCCT CCTTCACCTT CACCCCCACA GTGGCCTATC AGAGAGGCGG AGAGGCCGTC 
    AGCAGTGCAG GAAGGCCGGG TTTGTTGAAT ATAGGTGAAC CAGCTACACA GCCCTGGCTT 
    GCGGACACCT GGCCCAACAG CTGCTCCAAT CACAATGACT GCACTATTAA CTGCTGCACC 
    GGCAGCAATG GAAACAGCGA CAGCAACCTG GCAACTTACA GCCGCCCAGC CGACTGCATC 
    GCCAACTACA GCAACCAGAT GGACAACAAG GCCACCAACC TGCTGGCTCC AGATACTGGG 
    CTTTACAGCG ACGTTGACCT TTCAAACAAG ATCAACGAGA TGAAGACCTT CAACAGCCCC 
    AACCTGAAGG ACGGGCGTTT TATGGGGCCT GGTGGTCAAC CCACACCCTA CGCCACCACT 
    CAGCTCATTC AATCCAGCCT GGCCAACAAC AACATGAACA GCAGCAGTGG CGGAAGCGGA 
    GGAGGAAGCG CTGACTTTAA TGAGAAGCAG AGCTGGAAGA GCAGCCAGGG AACACCTCAG 
    AAACAGGCCG ACATCAACAC ACCACTGCAG TATAACGTCA TGGAACAAAA CAAACTCAAC 
    AAGGACCACT ACCGAGGTGA GGGTCCCATC TCAGTCACCA TCCCTTATAA CCAGGCCCAG 
    GACGTCCTCA CAGGAGAAAC CTACAGCAGC TCGGACAGAG GGAGCAGCAC CTCTGGAAGT 
    CAAGGGCAGA AAAAGGGTGG ACGAACAACT AAACAAGCCA AACAGAATCC AATGAATTGG 
    ACTGAACTGT TGCCTCCTCC TCCGGTTAAT CCGCCGCCAT GTCAAGAATA CACCCTGGTG 
    ATGGACGACA GTTACGACAC AGACCTGCAG TGCCCTTTGC CGCCGTCTCG GATGTATCTG 
    CAACCTGACG AGTTGGAAGA GGAGGAGGAC GAGGTGGAGC TGGAGCGTGG ACCTACACCT 
    CCTGTACGGG GAGCTGCTTC TTCACCTGCT GCCGTGTCCT ACAGCCACCA GTCCACCGCA 
    ACCCTAACCC CGTCCCCTCA GGAGGAGATG CATCCCATGC TGCAGGACAA CCCTGATCTA 
    CATGAGCGCA GGAGACTGTT AGTGAGTCCA CCTCCTCCAC CACGACCTCT CTCTCCTCCA 
    CACACATACG GCTACATCTC CAGTCCCCTC GGCCTGGACA CGGACGGCCT AGAGGAAGAG 
    GATGATGATG AAGAAGAGGG TGACGAAACG GACGCAGAGG TAGCTCGAGT CCACCAGCAC 
    CACATGATGC ACCCTCACCA GCAGCACTTG CCCCAGCAGA GGGCTCTCCT GCGGGGTCTG 
    GAACAAACCC CGGCCTCCAG CACCTGTGAC CTGGAGAGCT CTGTGACTGG GTCTATGATT 
    AACGGATGGG GTTCGGCTTC AGAAGAGGAC AACGCCTCGT CAGGCCGTTC CAGTGCAGTC 
    AGCTCATCGG ATGGATCGTT CTTTACCGAT GCCGATTTCG CCCAGGCTGT GGCAGCTGCG 
    GCTGAATATG CTGGTCTTCG AGTGGCAAAG CATCCTGGGA AAGGACATCA TCAGCTTCCA 
    GTGTCTGGAG CAGCACGGAA ATACCAAAAC CTCCCTGCGG GACACCGTCC AGGCAGCCCA 
    GTGTCCACAG ACAGTAACGT TAGCGCAGCC CCGGGTCAGA GGAGGCCCCT GAAGAGGCAA 
    AAACAACATC CAGCTGCTCA GACAGGCTAC CAGCGCCGAG AGGCCTACAC AGAGGATCTC 
    CCCCCTCCTC CGATTCCTCC TCCAGCCATG CTGAAGTCTC CAACACACCC GGCCAAAACC 
    ATCCCCGAGG GTCCACGGGG GGCCATGTCG CCAAGATCCA GCTCTGCGGA TGGCAAGGAC 
    AGGCGAACGG GTGCTGGCGG GCCCAGGGTC AGGGACGGTT CAGACACTCG GACCAGCTCT 
    TCAGAGCGGA GGGATGGCCA AGATGTACAG AGGGGTCAGA GAACGGGCAA GAATAGCAAA 
    CAAGATGAAG CCGTGAAGAG CCGCCAGCAC CCAGGCTCAG AGGACATCCT ACCTTACAGT 
    CGTCCTTCTT TCCCAGCCGT CCACAGTCCT CGAGGAGAAC GGGACCCCAG TTCCTCCAGT 
    TCACTGTCCT CTCGGGGGTC TGGCGGGCGA CGGAGAGGTG AAGGGCCACC AGGGGCCCGC 
    AGAAACCCTG CAGACATTGG GCTAAACACC ATAGGAGCCT TCCAGACTGG AGGGGACGAG 
    CTTGAGATGG TGGAGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214695.1
    CDS118..5238
    Translation

    Target ORF information:

    RefSeq Version XM_017359206.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359206.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCAGTTCA GAGATTGCAA TTGCAGATTT TTTATACGGA ACTGTGTCCT GCTTATGGTT 
    TGGTATCAGG GGTATTGGGG CGCCACTATG TTGCAGATGG CCCGATACAC TTTACTGGAG 
    GACTCAAAGT CTGTATTACT TCCAACAGAC ACGGGGACTC AGGATGGGAC TAGCAATAGG 
    GCCACAGAGA CCCCCACTGC AGAACACGTT GACGACAACT CTCTGGGCTA CACAGGATCC 
    CGTCTGCGGC AGGAAGATTA CGCACCCCGT ATTGTTGAAC ACCCCTCTGA TCTGATCGTA 
    TCCAAAGGCG AACCGGCCAC CCTAAACTGC AAGGCTGAAG GACGTCCCAC GCCCACAGTG 
    GAGTGGTACA AAGATGGAGA ACGAGTTGAA ACCGACCGGG ACAATCCTCG ATCCCACCGC 
    ATGCTCCTGC CCACCGGATC TCTCTTCTTT CTCCGCATTG TTCACGGCCG GCGCAGCAAA 
    CCCGACGAAG GCTCCTACGT TTGTGTGGCA CGTAACTATC TGGGAGAAGC CGTCAGCCAC 
    AACGCCTCGC TGGAAGTAGC TATTCTCCGA GATGATTTCC GACAGAACCC TGTGGACGTG 
    ATGGTGGCAG CGGGGGAGCC AGCTGTAATG GAGTGCCAGC CGCCCCGAGG ACACCCAGAG 
    CCCACCATCT CCTGGAAGAA AGATGGGGTC AATATAGACG ACAGAGACGA GAGGATAACG 
    ATACGAGGAG GAAAGCTCAT GATCACCAAC ACCAGAAAAA CGGACGCGGG GAAGTACGTG 
    TGCGTGGGAA CCAACATGAT GGGGGAACGA GAGAGCGAGA TCGCGGAGTT GACTGTTCTC 
    GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT 
    GAGTTCAGAT GTGAAGCTCA AGGAGACCCC GTGCCCACCA TCCGATGGAG GAAAGAGGAC 
    GGAGAACTGC CCAAGGGAAG GTATGAGATC TGGGAGGACC ACACTCTCAA ACTGAAGAGG 
    CTGACTTCAG GAGACGCCGG TACTTACACC TGTCAGGCGG AGAACATGAT GGGCAAGACG 
    GAGGCCAGCG CCACCCTCAC CGTCCACGTT CCTCCAGTGT TTGTGGTCCG ACCCAGGAAT 
    CAGGTGGCGG GTATGGGCCG CACCGTCACC TTTCAGTGTG AAGCCACAGG AAGTCCTCAG 
    CCTGCAATAT TCTGGCAGAA AGAAGGCAGT CAGAATCTCC TGTTCTCGTC TCAGCCTCCT 
    CCTCCGTCCA GCAGATTCTC TGTGTCTCAG ACTGGAGATC TGACCATCAC AGCAGTTGAG 
    CGTTCAGATG GAGGATATTA TAGCTGTCAG GCTCTCAACA TCGCTGGCAG TGTGATCACC 
    AAAGCCCTGC TGGAGGTTAC AGACGTGATT TCAGACAGGC CTCCACCAAT CGTCCGTCAG 
    GGTCCAACCA ACCAGACTGT GGCAGTGGAC GGGACGGTGG TTCTGAGCTG CCAAGCCACC 
    GGGACACCGA TTCCTACCAT CTTATGGAGG AAAGATGGAG TTCTGGTGTC CACACATGAT 
    TCCCGACTCA AGCAGCTGGA CACTGGAGCC CTGCAGATTC GATACGCAAA GCTGGGCGAC 
    ACTGGCATGT ACACCTGCAC GGCTTCCACC CCGAGCGGAG AGGCGTCCTG GAAGGCCTAC 
    CTGGAGGTGC AAGAGTTTGG TGCTCCAGTT CAACCGGGCC GTCCCACAGA TCCCAACCTC 
    ATCCCCAGCG CCCCATCTAA ACCAGACGTC ACGGATGTCA GCCGCACCTC CGTGTCTCTT 
    TCCTGGAAGC CGAATCTGAA CGCTGGAGCC ACACCCACCT CATACGTCAT TGAGGCCTTC 
    AGCCATGCAG CAGGCAGCAG CTGGCAGACG CTCGCCGATC ATGTGAAGAC GGAAAGCTTC 
    GTGCTTAAGG GTCTGAAGCC AAGTGCCGTC TACCTGTTTC TGGTGAGAGC GGCCAATGCG 
    TACGGCCTGA GCGACCCCAG TCCCATCAGC GACGCTGTCA AAACACAAGA CGTCCCACCA 
    ACCAGCCAGG GAGTGGAGCA TGGCCAGATC CAGAGAGAAC TGGGAGATGT CGTCATTCAC 
    CTTCACAATC CCACAATTCT CTCATCGTCT TCCGTCAGAG TCCAGTGGAC TGTGGAGCAG 
    CAGTCTCAGT ATATTCAGGG CTATAAGGTG ATGTACCGTC CGTCTCCAGA AGGCGCTCCG 
    CAGCGAGTGG ATTGGGCCAT GTTTGAGGTC GGAGCCCCGG GGGAGCACAG CGCTGTCGTC 
    ACCCAGCTCA AGAAGGGCAT CACCTATGAG TTCAAAGTGC GCCCTTTCTT CAATGAGTTC 
    CAGGGCACCG ACAGCGACAT CAAAGTGGGA AAGACCCTGG AGGAGGCTCC CAGCGCTGCT 
    CCCAGAGGCG TCACCGTAAC AGGAAGTGGA GACAACGGCA CAGCAGTCCT GGTCGCCTGG 
    CAACCGCCGC CAGAGGAGGA GCAGAACGGC GTTGTGCAGG AGTACAAGAT CTGGTGTCTT 
    GGTAATGAAA GCCGATACCA CATCAACCGC ACAGTGGATG GTTCTACACA CTCGGTGTTG 
    ATTCCTGGTC TGGTGGCAGG AGTCACATAC AGGCTAGAGG TGGCAGCAGG CACTGGGGCC 
    GGACCCGGGG TCAAGAGCGA CCCTACGTCC TTCCAGCTTG ACTCTTCTGG CCGTGTTCTC 
    CCGCCGCCGC TGGACGACGA TAACACCATT TCCCAGCAGA TCTCAGACGT GGTGCGTCAG 
    CCGGCTTTCA TCGCGGGCAT CGGCGCCGCC TGCTGGATCA TCCTGATGGT GTTCAGCATC 
    TGGCTATACC GCCACCGCAA GAAGAGGAAC GGCCTCAGCA GCAGCTACGC CGGCATCCGC 
    AAAGTGCCCT CCTTCACCTT CACCCCCACA GTGGCCTATC AGAGAGGCGG AGAGGCCGTC 
    AGCAGTGCAG GAAGGCCGGG TTTGTTGAAT ATAGGTGAAC CAGCTACACA GCCCTGGCTT 
    GCGGACACCT GGCCCAACAG CTGCTCCAAT CACAATGACT GCACTATTAA CTGCTGCACC 
    GGCAGCAATG GAAACAGCGA CAGCAACCTG GCAACTTACA GCCGCCCAGC CGACTGCATC 
    GCCAACTACA GCAACCAGAT GGACAACAAG GCCACCAACC TGCTGGCTCC AGATACTGGG 
    CTTTACAGCG ACGTTGACCT TTCAAACAAG ATCAACGAGA TGAAGACCTT CAACAGCCCC 
    AACCTGAAGG ACGGGCGTTT TATGGGGCCT GGTGGTCAAC CCACACCCTA CGCCACCACT 
    CAGCTCATTC AATCCAGCCT GGCCAACAAC AACATGAACA GCAGCAGTGG CGGAAGCGGA 
    GGAGGAAGCG CTGACTTTAA TGAGAAGCAG AGCTGGAAGA GCAGCCAGGG AACACCTCAG 
    AAACAGGCCG ACATCAACAC ACCACTGCAG TATAACGTCA TGGAACAAAA CAAACTCAAC 
    AAGGACCACT ACCGAGGTGA GGGTCCCATC TCAGTCACCA TCCCTTATAA CCAGGCCCAG 
    GACGTCCTCA CAGGAGAAAC CTACAGCAGC TCGGACAGAG GGAGCAGCAC CTCTGGAAGT 
    CAAGGGCAGA AAAAGGGTGG ACGAACAACT AAACAAGCCA AACAGAATCC AATGAATTGG 
    ACTGAACTGT TGCCTCCTCC TCCGGTTAAT CCGCCGCCAT GTCAAGAATA CACCCTGGTG 
    ATGGACGACA GTTACGACAC AGACCTGCAG TGCCCTTTGC CGCCGTCTCG GATGTATCTG 
    CAACCTGACG AGTTGGAAGA GGAGGAGGAC GAGGTGGAGC TGGAGCGTGG ACCTACACCT 
    CCTGTACGGG GAGCTGCTTC TTCACCTGCT GCCGTGTCCT ACAGCCACCA GTCCACCGCA 
    ACCCTAACCC CGTCCCCTCA GGAGGAGATG CATCCCATGC TGCAGGACAA CCCTGATCTA 
    CATGAGCGCA GGAGACTGTT AGTGAGTCCA CCTCCTCCAC CACGACCTCT CTCTCCTCCA 
    CACACATACG GCTACATCTC CAGTCCCCTC GGCCTGGACA CGGACGGCCT AGAGGAAGAG 
    GATGATGATG AAGAAGAGGG TGACGAAACG GACGCAGAGG TAGCTCGAGT CCACCAGCAC 
    CACATGATGC ACCCTCACCA GCAGCACTTG CCCCAGCAGA GGGCTCTCCT GCGGGGTCTG 
    GAACAAACCC CGGCCTCCAG CACCTGTGAC CTGGAGAGCT CTGTGACTGG GTCTATGATT 
    AACGGATGGG GTTCGGCTTC AGAAGAGGAC AACGCCTCGT CAGGCCGTTC CAGTGCAGTC 
    AGCTCATCGG ATGGATCGTT CTTTACCGAT GCCGATTTCG CCCAGGCTGT GGCAGCTGCG 
    GCTGAATATG CTGGTCTTCG AGTGGCAAAG CATCCTGGGA AAGGACATCA TCAGCTTCCA 
    GTGTCTGGAG CAGCACGGAA ATACCAAAAC CTCCCTGCGG GACACCGTCC AGGCAGCCCA 
    GTGTCCACAG ACAGTAACGT TAGCGCAGCC CCGGGTCAGA GGAGGCCCCT GAAGAGGCAA 
    AAACAACATC CAGCTGCTCA GACAGGCTAC CAGCGCCGAG AGGCCTACAC AGAGGATCTC 
    CCCCCTCCTC CGATTCCTCC TCCAGCCATG CTGAAGTCTC CAACACACCC GGCCAAAACC 
    ATCCCCGAGG GTCCACGGGG GGCCATGTCG CCAAGATCCA GCTCTGCGGA TGGCAAGGAC 
    AGGCGAACGG GTGCTGGCGG GCCCAGGGTC AGGGACGGTT CAGACACTCG GACCAGCTCT 
    TCAGAGCGGA GGGATGGCCA AGATGTACAG AGGGGTCAGA GAACGGGCAA GAATAGCAAA 
    CAAGATGAAG CCGTGAAGAG CCGCCAGCAC CCAGGCTCAG AGGACATCCT ACCTTACAGT 
    CGTCCTTCTT TCCCAGCCGT CCACAGTCCT CGAGGAGAAC GGGACCCCAG TTCCTCCAGT 
    TCACTGTCCT CTCGGGGGTC TGGCGGGCGA CGGAGAGGTG AAGGGCCACC AGGGGCCCGC 
    AGAAACCCTG CAGACATTGG GCTAAACACC ATAGGAGCCT TCCAGACTGG AGGGGACGAG 
    CTTGAGATGG TGGAGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359207.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214696.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa55058
    Clone ID Related Accession (Same CDS sequence) XM_017359207.1
    Accession Version XM_017359207.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCAGTTCA GAGATTGCAA TTGCAGATTT TTTATACGGA ACTGTGTCCT GCTTATGGTT 
    TGGTATCAGG GGTATTGGGG CGCCACTATG TTGCAGATGG CCCGATACAC TTTACTGGAG 
    GACTCAAAGT CTGTATTACT TCCAACAGAC ACGGGGACTC AGGATGGGAC TAGCAATAGG 
    GCCACAGAGA CCCCCACTGC AGAACACGTT GACGACAACT CTCTGGGCTA CACAGGATCC 
    CGTCTGCGGC AGGAAGATTA CGCACCCCGT ATTGTTGAAC ACCCCTCTGA TCTGATCGTA 
    TCCAAAGGCG AACCGGCCAC CCTAAACTGC AAGGCTGAAG GACGTCCCAC GCCCACAGTG 
    GAGTGGTACA AAGATGGAGA ACGAGTTGAA ACCGACCGGG ACAATCCTCG ATCCCACCGC 
    ATGCTCCTGC CCACCGGATC TCTCTTCTTT CTCCGCATTG TTCACGGCCG GCGCAGCAAA 
    CCCGACGAAG GCTCCTACGT TTGTGTGGCA CGTAACTATC TGGGAGAAGC CGTCAGCCAC 
    AACGCCTCGC TGGAAGTAGC TATTCTCCGA GATGATTTCC GACAGAACCC TGTGGACGTG 
    ATGGTGGCAG CGGGGGAGCC AGCTGTAATG GAGTGCCAGC CGCCCCGAGG ACACCCAGAG 
    CCCACCATCT CCTGGAAGAA AGATGGGGTC AATATAGACG ACAGAGACGA GAGGATAACG 
    ATACGAGGAG GAAAGCTCAT GATCACCAAC ACCAGAAAAA CGGACGCGGG GAAGTACGTG 
    TGCGTGGGAA CCAACATGAT GGGGGAACGA GAGAGCGAGA TCGCGGAGTT GACTGTTCTC 
    GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT 
    GAGTTCAGAT GTGAAGCTCA AGGAGACCCC GTGCCCACCA TCCGATGGAG GAAAGAGGAC 
    GGAGAACTGC CCAAGGGAAG GTATGAGATC TGGGAGGACC ACACTCTCAA ACTGAAGAGG 
    CTGACTTCAG GAGACGCCGG TACTTACACC TGTCAGGCGG AGAACATGAT GGGCAAGACG 
    GAGGCCAGCG CCACCCTCAC CGTCCACGTT CCTCCAGTGT TTGTGGTCCG ACCCAGGAAT 
    CAGGTGGCGG GTATGGGCCG CACCGTCACC TTTCAGTGTG AAGCCACAGG AAGTCCTCAG 
    CCTGCAATAT TCTGGCAGAA AGAAGGCAGT CAGAATCTCC TGTTCTCGTC TCAGCCTCCT 
    CCTCCGTCCA GCAGATTCTC TGTGTCTCAG ACTGGAGATC TGACCATCAC AGCAGTTGAG 
    CGTTCAGATG GAGGATATTA TAGCTGTCAG GCTCTCAACA TCGCTGGCAG TGTGATCACC 
    AAAGCCCTGC TGGAGGTTAC AGACGTGATT TCAGACAGGC CTCCACCAAT CGTCCGTCAG 
    GGTCCAACCA ACCAGACTGT GGCAGTGGAC GGGACGGTGG TTCTGAGCTG CCAAGCCACC 
    GGGACACCGA TTCCTACCAT CTTATGGAGG AAAGATGGAG TTCTGGTGTC CACACATGAT 
    TCCCGACTCA AGCAGCTGGA CACTGGAGCC CTGCAGATTC GATACGCAAA GCTGGGCGAC 
    ACTGGCATGT ACACCTGCAC GGCTTCCACC CCGAGCGGAG AGGCGTCCTG GAAGGCCTAC 
    CTGGAGGTGC AAGAGTTTGG TGCTCCAGTT CAACCGGGCC GTCCCACAGA TCCCAACCTC 
    ATCCCCAGCG CCCCATCTAA ACCAGACGTC ACGGATGTCA GCCGCACCTC CGTGTCTCTT 
    TCCTGGAAGC CGAATCTGAA CGCTGGAGCC ACACCCACCT CATACGTCAT TGAGGCCTTC 
    AGCCATGCAG CAGGCAGCAG CTGGCAGACG CTCGCCGATC ATGTGAAGAC GGAAAGCTTC 
    GTGCTTAAGG GTCTGAAGCC AAGTGCCGTC TACCTGTTTC TGGTGAGAGC GGCCAATGCG 
    TACGGCCTGA GCGACCCCAG TCCCATCAGC GACGCTGTCA AAACACAAGA CGTCCCACCA 
    ACCAGCCAGG GAGTGGAGCA TGGCCAGATC CAGAGAGAAC TGGGAGATGT CGTCATTCAC 
    CTTCACAATC CCACAATTCT CTCATCGTCT TCCGTCAGAG TCCAGTGGAC TGTGGAGCAG 
    CAGTCTCAGT ATATTCAGGG CTATAAGGTG ATGTACCGTC CGTCTCCAGA AGGCGCTCCG 
    CAGCGAGTGG ATTGGGCCAT GTTTGAGGTC GGAGCCCCGG GGGAGCACAG CGCTGTCGTC 
    ACCCAGCTCA AGAAGGGCAT CACCTATGAG TTCAAAGTGC GCCCTTTCTT CAATGAGTTC 
    CAGGGCACCG ACAGCGACAT CAAAGTGGGA AAGACCCTGG AGGAGGCTCC CAGCGCTGCT 
    CCCAGAGGCG TCACCGTAAC AGGAAGTGGA GACAACGGCA CAGCAGTCCT GGTCGCCTGG 
    CAACCGCCGC CAGAGGAGGA GCAGAACGGC GTTGTGCAGG AGTACAAGAT CTGGTGTCTT 
    GGTAATGAAA GCCGATACCA CATCAACCGC ACAGTGGATG GTTCTACACA CTCGGTGTTG 
    ATTCCTGGTC TGGTGGCAGG AGTCACATAC AGGCTAGAGG TGGCAGCAGG CACTGGGGCC 
    GGACCCGGGG TCAAGAGCGA CCCTACGTCC TTCCAGCTTG ACTCTTCTGG CCGTGTTCTC 
    CCGCCGCCGC TGGACGACGA TAACACCATT TCCCAGCAGA TCTCAGACGT GGTGCGTCAG 
    CCGGCTTTCA TCGCGGGCAT CGGCGCCGCC TGCTGGATCA TCCTGATGGT GTTCAGCATC 
    TGGCTATACC GCCACCGCAA GAAGAGGAAC GGCCTCAGCA GCAGCTACGC CGGCATCCGC 
    AAAGTGCCCT CCTTCACCTT CACCCCCACA GTGGCCTATC AGAGAGGCGG AGAGGCCGTC 
    AGCAGTGCAG GAAGGCCGGG TTTGTTGAAT ATAGGTGAAC CAGCTACACA GCCCTGGCTT 
    GCGGACACCT GGCCCAACAG CTGCTCCAAT CACAATGACT GCACTATTAA CTGCTGCACC 
    GGCAGCAATG GAAACAGCGA CAGCAACCTG GCAACTTACA GCCGCCCAGC CGACTGCATC 
    GCCAACTACA GCAACCAGAT GGACAACAAG GCCACCAACC TGCTGGCTCC AGATACTGGG 
    CTTTACAGCG ACGTTGACCT TTCAAACAAG ATCAACGAGA TGAAGACCTT CAACAGCCCC 
    AACCTGAAGG ACGGGCGTTT TATGGGGCCT GGTGGTCAAC CCACACCCTA CGCCACCACT 
    CAGCTCATTC AATCCAGCCT GGCCAACAAC AACATGAACA GCAGCAGTGG CGGAAGCGGA 
    GGAGGAAGCG CTGACTTTAA TGAGAAGCAG AGCTGGAAGA GCAGCCAGGG AACACCTCAG 
    AAACAGGCCG ACATCAACAC ACCACTGCAG TATAACGTCA TGGAACAAAA CAAACTCAAC 
    AAGGACCACT ACCGAGGTGA GGGTCCCATC TCAGTCACCA TCCCTTATAA CCAGGCCCAG 
    GACGTCCTCA CAGGAGAAAC CTACAGCAGC TCGGACAGAG GGAGCAGCAC CTCTGGAAGT 
    CAAGGGCAGA AAAAGGGTGG ACGAACAACT AAACAAGCCA AACAGAATCC AATGAATTGG 
    ACTGAACTGT TGCCTCCTCC TCCGGTTAAT CCGCCGCCAT GTCAAGAATA CACCCTGGTG 
    ATGGACGACA GTTACGACAC AGACCTGCAG TGCCCTTTGC CGCCGTCTCG GATGTATCTG 
    CAACCTGACG AGTTGGAAGA GGAGGAGGAC GAGGTGGAGC TGGAGCGTGG ACCTACACCT 
    CCTGTACGGG GAGCTGCTTC TTCACCTGCT GCCGTGTCCT ACAGCCACCA GTCCACCGCA 
    ACCCTAACCC CGTCCCCTCA GGAGGAGATG CATCCCATGC TGCAGGACAA CCCTGATCTA 
    CATGAGCGCA GGAGACTGTT AGTGAGTCCA CCTCCTCCAC CACGACCTCT CTCTCCTCCA 
    CACACATACG GCTACATCTC CAGTCCCCTC GGCCTGGACA CGGACGGCCT AGAGGAAGAG 
    GATGATGATG AAGAAGAGGG TGACGAAACG GACGCAGAGG TAGCTCGAGT CCACCAGCAC 
    CACATGATGC ACCCTCACCA GCAGCACTTG CCCCAGCAGA GGGCTCTCCT GCGGGGTCTG 
    GAACAAACCC CGGCCTCCAG CACCTGTGAC CTGGAGAGCT CTGTGACTGG GTCTATGATT 
    AACGGATGGG GTTCGGCTTC AGAAGAGGAC AACGCCTCGT CAGGCCGTTC CAGTGCAGTC 
    AGCTCATCGG ATGGATCGTT CTTTACCGAT GCCGATTTCG CCCAGGCTGT GGCAGCTGCG 
    GCTGAATATG CTGGTCTTCG AGTGGCAAAG CATCCTGGGA AAGGACATCA TCAGCTTCCA 
    GTGTCTGGAG CACGGAAATA CCAAAACCTC CCTGCGGGAC ACCGTCCAGG CAGCCCAGTG 
    TCCACAGACA GTAACGTTAG CGCAGCCCCG GGTCAGAGGA GGCCCCTGAA GAGGCAAAAA 
    CAACATCCAG CTGCTCAGAC AGGCTACCAG CGCCGAGAGG CCTACACAGA GGATCTCCCC 
    CCTCCTCCGA TTCCTCCTCC AGCCATGCTG AAGTCTCCAA CACACCCGGC CAAAACCATC 
    CCCGAGGGTC CACGGGGGGC CATGTCGCCA AGATCCAGCT CTGCGGATGG CAAGGACAGG 
    CGAACGGGTG CTGGCGGGCC CAGGGTCAGG GACGGTTCAG ACACTCGGAC CAGCTCTTCA 
    GAGCGGAGGG ATGGCCAAGA TGTACAGAGG GGTCAGAGAA CGGGCAAGAA TAGCAAACAA 
    GATGAAGCCG TGAAGAGCCG CCAGCACCCA GGCTCAGAGG ACATCCTACC TTACAGTCGT 
    CCTTCTTTCC CAGCCGTCCA CAGTCCTCGA GGAGAACGGG ACCCCAGTTC CTCCAGTTCA 
    CTGTCCTCTC GGGGGTCTGG CGGGCGACGG AGAGGTGAAG GGCCACCAGG GGCCCGCAGA 
    AACCCTGCAG ACATTGGGCT AAACACCATA GGAGCCTTCC AGACTGGAGG GGACGAGCTT 
    GAGATGGTGG AGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214696.1
    CDS116..5233
    Translation

    Target ORF information:

    RefSeq Version XM_017359207.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359207.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCAGTTCA GAGATTGCAA TTGCAGATTT TTTATACGGA ACTGTGTCCT GCTTATGGTT 
    TGGTATCAGG GGTATTGGGG CGCCACTATG TTGCAGATGG CCCGATACAC TTTACTGGAG 
    GACTCAAAGT CTGTATTACT TCCAACAGAC ACGGGGACTC AGGATGGGAC TAGCAATAGG 
    GCCACAGAGA CCCCCACTGC AGAACACGTT GACGACAACT CTCTGGGCTA CACAGGATCC 
    CGTCTGCGGC AGGAAGATTA CGCACCCCGT ATTGTTGAAC ACCCCTCTGA TCTGATCGTA 
    TCCAAAGGCG AACCGGCCAC CCTAAACTGC AAGGCTGAAG GACGTCCCAC GCCCACAGTG 
    GAGTGGTACA AAGATGGAGA ACGAGTTGAA ACCGACCGGG ACAATCCTCG ATCCCACCGC 
    ATGCTCCTGC CCACCGGATC TCTCTTCTTT CTCCGCATTG TTCACGGCCG GCGCAGCAAA 
    CCCGACGAAG GCTCCTACGT TTGTGTGGCA CGTAACTATC TGGGAGAAGC CGTCAGCCAC 
    AACGCCTCGC TGGAAGTAGC TATTCTCCGA GATGATTTCC GACAGAACCC TGTGGACGTG 
    ATGGTGGCAG CGGGGGAGCC AGCTGTAATG GAGTGCCAGC CGCCCCGAGG ACACCCAGAG 
    CCCACCATCT CCTGGAAGAA AGATGGGGTC AATATAGACG ACAGAGACGA GAGGATAACG 
    ATACGAGGAG GAAAGCTCAT GATCACCAAC ACCAGAAAAA CGGACGCGGG GAAGTACGTG 
    TGCGTGGGAA CCAACATGAT GGGGGAACGA GAGAGCGAGA TCGCGGAGTT GACTGTTCTC 
    GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT 
    GAGTTCAGAT GTGAAGCTCA AGGAGACCCC GTGCCCACCA TCCGATGGAG GAAAGAGGAC 
    GGAGAACTGC CCAAGGGAAG GTATGAGATC TGGGAGGACC ACACTCTCAA ACTGAAGAGG 
    CTGACTTCAG GAGACGCCGG TACTTACACC TGTCAGGCGG AGAACATGAT GGGCAAGACG 
    GAGGCCAGCG CCACCCTCAC CGTCCACGTT CCTCCAGTGT TTGTGGTCCG ACCCAGGAAT 
    CAGGTGGCGG GTATGGGCCG CACCGTCACC TTTCAGTGTG AAGCCACAGG AAGTCCTCAG 
    CCTGCAATAT TCTGGCAGAA AGAAGGCAGT CAGAATCTCC TGTTCTCGTC TCAGCCTCCT 
    CCTCCGTCCA GCAGATTCTC TGTGTCTCAG ACTGGAGATC TGACCATCAC AGCAGTTGAG 
    CGTTCAGATG GAGGATATTA TAGCTGTCAG GCTCTCAACA TCGCTGGCAG TGTGATCACC 
    AAAGCCCTGC TGGAGGTTAC AGACGTGATT TCAGACAGGC CTCCACCAAT CGTCCGTCAG 
    GGTCCAACCA ACCAGACTGT GGCAGTGGAC GGGACGGTGG TTCTGAGCTG CCAAGCCACC 
    GGGACACCGA TTCCTACCAT CTTATGGAGG AAAGATGGAG TTCTGGTGTC CACACATGAT 
    TCCCGACTCA AGCAGCTGGA CACTGGAGCC CTGCAGATTC GATACGCAAA GCTGGGCGAC 
    ACTGGCATGT ACACCTGCAC GGCTTCCACC CCGAGCGGAG AGGCGTCCTG GAAGGCCTAC 
    CTGGAGGTGC AAGAGTTTGG TGCTCCAGTT CAACCGGGCC GTCCCACAGA TCCCAACCTC 
    ATCCCCAGCG CCCCATCTAA ACCAGACGTC ACGGATGTCA GCCGCACCTC CGTGTCTCTT 
    TCCTGGAAGC CGAATCTGAA CGCTGGAGCC ACACCCACCT CATACGTCAT TGAGGCCTTC 
    AGCCATGCAG CAGGCAGCAG CTGGCAGACG CTCGCCGATC ATGTGAAGAC GGAAAGCTTC 
    GTGCTTAAGG GTCTGAAGCC AAGTGCCGTC TACCTGTTTC TGGTGAGAGC GGCCAATGCG 
    TACGGCCTGA GCGACCCCAG TCCCATCAGC GACGCTGTCA AAACACAAGA CGTCCCACCA 
    ACCAGCCAGG GAGTGGAGCA TGGCCAGATC CAGAGAGAAC TGGGAGATGT CGTCATTCAC 
    CTTCACAATC CCACAATTCT CTCATCGTCT TCCGTCAGAG TCCAGTGGAC TGTGGAGCAG 
    CAGTCTCAGT ATATTCAGGG CTATAAGGTG ATGTACCGTC CGTCTCCAGA AGGCGCTCCG 
    CAGCGAGTGG ATTGGGCCAT GTTTGAGGTC GGAGCCCCGG GGGAGCACAG CGCTGTCGTC 
    ACCCAGCTCA AGAAGGGCAT CACCTATGAG TTCAAAGTGC GCCCTTTCTT CAATGAGTTC 
    CAGGGCACCG ACAGCGACAT CAAAGTGGGA AAGACCCTGG AGGAGGCTCC CAGCGCTGCT 
    CCCAGAGGCG TCACCGTAAC AGGAAGTGGA GACAACGGCA CAGCAGTCCT GGTCGCCTGG 
    CAACCGCCGC CAGAGGAGGA GCAGAACGGC GTTGTGCAGG AGTACAAGAT CTGGTGTCTT 
    GGTAATGAAA GCCGATACCA CATCAACCGC ACAGTGGATG GTTCTACACA CTCGGTGTTG 
    ATTCCTGGTC TGGTGGCAGG AGTCACATAC AGGCTAGAGG TGGCAGCAGG CACTGGGGCC 
    GGACCCGGGG TCAAGAGCGA CCCTACGTCC TTCCAGCTTG ACTCTTCTGG CCGTGTTCTC 
    CCGCCGCCGC TGGACGACGA TAACACCATT TCCCAGCAGA TCTCAGACGT GGTGCGTCAG 
    CCGGCTTTCA TCGCGGGCAT CGGCGCCGCC TGCTGGATCA TCCTGATGGT GTTCAGCATC 
    TGGCTATACC GCCACCGCAA GAAGAGGAAC GGCCTCAGCA GCAGCTACGC CGGCATCCGC 
    AAAGTGCCCT CCTTCACCTT CACCCCCACA GTGGCCTATC AGAGAGGCGG AGAGGCCGTC 
    AGCAGTGCAG GAAGGCCGGG TTTGTTGAAT ATAGGTGAAC CAGCTACACA GCCCTGGCTT 
    GCGGACACCT GGCCCAACAG CTGCTCCAAT CACAATGACT GCACTATTAA CTGCTGCACC 
    GGCAGCAATG GAAACAGCGA CAGCAACCTG GCAACTTACA GCCGCCCAGC CGACTGCATC 
    GCCAACTACA GCAACCAGAT GGACAACAAG GCCACCAACC TGCTGGCTCC AGATACTGGG 
    CTTTACAGCG ACGTTGACCT TTCAAACAAG ATCAACGAGA TGAAGACCTT CAACAGCCCC 
    AACCTGAAGG ACGGGCGTTT TATGGGGCCT GGTGGTCAAC CCACACCCTA CGCCACCACT 
    CAGCTCATTC AATCCAGCCT GGCCAACAAC AACATGAACA GCAGCAGTGG CGGAAGCGGA 
    GGAGGAAGCG CTGACTTTAA TGAGAAGCAG AGCTGGAAGA GCAGCCAGGG AACACCTCAG 
    AAACAGGCCG ACATCAACAC ACCACTGCAG TATAACGTCA TGGAACAAAA CAAACTCAAC 
    AAGGACCACT ACCGAGGTGA GGGTCCCATC TCAGTCACCA TCCCTTATAA CCAGGCCCAG 
    GACGTCCTCA CAGGAGAAAC CTACAGCAGC TCGGACAGAG GGAGCAGCAC CTCTGGAAGT 
    CAAGGGCAGA AAAAGGGTGG ACGAACAACT AAACAAGCCA AACAGAATCC AATGAATTGG 
    ACTGAACTGT TGCCTCCTCC TCCGGTTAAT CCGCCGCCAT GTCAAGAATA CACCCTGGTG 
    ATGGACGACA GTTACGACAC AGACCTGCAG TGCCCTTTGC CGCCGTCTCG GATGTATCTG 
    CAACCTGACG AGTTGGAAGA GGAGGAGGAC GAGGTGGAGC TGGAGCGTGG ACCTACACCT 
    CCTGTACGGG GAGCTGCTTC TTCACCTGCT GCCGTGTCCT ACAGCCACCA GTCCACCGCA 
    ACCCTAACCC CGTCCCCTCA GGAGGAGATG CATCCCATGC TGCAGGACAA CCCTGATCTA 
    CATGAGCGCA GGAGACTGTT AGTGAGTCCA CCTCCTCCAC CACGACCTCT CTCTCCTCCA 
    CACACATACG GCTACATCTC CAGTCCCCTC GGCCTGGACA CGGACGGCCT AGAGGAAGAG 
    GATGATGATG AAGAAGAGGG TGACGAAACG GACGCAGAGG TAGCTCGAGT CCACCAGCAC 
    CACATGATGC ACCCTCACCA GCAGCACTTG CCCCAGCAGA GGGCTCTCCT GCGGGGTCTG 
    GAACAAACCC CGGCCTCCAG CACCTGTGAC CTGGAGAGCT CTGTGACTGG GTCTATGATT 
    AACGGATGGG GTTCGGCTTC AGAAGAGGAC AACGCCTCGT CAGGCCGTTC CAGTGCAGTC 
    AGCTCATCGG ATGGATCGTT CTTTACCGAT GCCGATTTCG CCCAGGCTGT GGCAGCTGCG 
    GCTGAATATG CTGGTCTTCG AGTGGCAAAG CATCCTGGGA AAGGACATCA TCAGCTTCCA 
    GTGTCTGGAG CACGGAAATA CCAAAACCTC CCTGCGGGAC ACCGTCCAGG CAGCCCAGTG 
    TCCACAGACA GTAACGTTAG CGCAGCCCCG GGTCAGAGGA GGCCCCTGAA GAGGCAAAAA 
    CAACATCCAG CTGCTCAGAC AGGCTACCAG CGCCGAGAGG CCTACACAGA GGATCTCCCC 
    CCTCCTCCGA TTCCTCCTCC AGCCATGCTG AAGTCTCCAA CACACCCGGC CAAAACCATC 
    CCCGAGGGTC CACGGGGGGC CATGTCGCCA AGATCCAGCT CTGCGGATGG CAAGGACAGG 
    CGAACGGGTG CTGGCGGGCC CAGGGTCAGG GACGGTTCAG ACACTCGGAC CAGCTCTTCA 
    GAGCGGAGGG ATGGCCAAGA TGTACAGAGG GGTCAGAGAA CGGGCAAGAA TAGCAAACAA 
    GATGAAGCCG TGAAGAGCCG CCAGCACCCA GGCTCAGAGG ACATCCTACC TTACAGTCGT 
    CCTTCTTTCC CAGCCGTCCA CAGTCCTCGA GGAGAACGGG ACCCCAGTTC CTCCAGTTCA 
    CTGTCCTCTC GGGGGTCTGG CGGGCGACGG AGAGGTGAAG GGCCACCAGG GGCCCGCAGA 
    AACCCTGCAG ACATTGGGCT AAACACCATA GGAGCCTTCC AGACTGGAGG GGACGAGCTT 
    GAGATGGTGG AGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359208.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214697.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa55059
    Clone ID Related Accession (Same CDS sequence) XM_017359208.2 , XM_017359208.1 , NM_001365166.1
    Accession Version XM_017359208.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359208.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAGGTCA CATGGTCAGG CGCAGAAGTC CTCATCATTT TCACCTTGCT GCAGTTTGTC 
    CATCCAGAGC TTGCTGAGGG TCCTTTGGCT CCAGACACGG GGACTCAGGA TGGGACTAGC 
    AATAGGGCCA CAGAGACCCC CACTGCAGAA CACGTTGACG ACAACTCTCT GGGCTACACA 
    GGATCCCGTC TGCGGCAGGA AGATTACGCA CCCCGTATTG TTGAACACCC CTCTGATCTG 
    ATCGTATCCA AAGGCGAACC GGCCACCCTA AACTGCAAGG CTGAAGGACG TCCCACGCCC 
    ACAGTGGAGT GGTACAAAGA TGGAGAACGA GTTGAAACCG ACCGGGACAA TCCTCGATCC 
    CACCGCATGC TCCTGCCCAC CGGATCTCTC TTCTTTCTCC GCATTGTTCA CGGCCGGCGC 
    AGCAAACCCG ACGAAGGCTC CTACGTTTGT GTGGCACGTA ACTATCTGGG AGAAGCCGTC 
    AGCCACAACG CCTCGCTGGA AGTAGCTATT CTCCGAGATG ATTTCCGACA GAACCCTGTG 
    GACGTGATGG TGGCAGCGGG GGAGCCAGCT GTAATGGAGT GCCAGCCGCC CCGAGGACAC 
    CCAGAGCCCA CCATCTCCTG GAAGAAAGAT GGGGTCAATA TAGACGACAG AGACGAGAGG 
    ATAACGATAC GAGGAGGAAA GCTCATGATC ACCAACACCA GAAAAACGGA CGCGGGGAAG 
    TACGTGTGCG TGGGAACCAA CATGATGGGG GAACGAGAGA GCGAGATCGC GGAGTTGACT 
    GTTCTCGAGA GGCCCAGTTT CTTGCGAAAG CCGAGCAGCC AGTCCGTCCT TGTGGATCAG 
    AGCGTTGAGT TCAGATGTGA AGCTCAAGGA GACCCCGTGC CCACCATCCG ATGGAGGAAA 
    GAGGACGGAG AACTGCCCAA GGGAAGGTAT GAGATCTGGG AGGACCACAC TCTCAAACTG 
    AAGAGGCTGA CTTCAGGAGA CGCCGGTACT TACACCTGTC AGGCGGAGAA CATGATGGGC 
    AAGACGGAGG CCAGCGCCAC CCTCACCGTC CACGTTGTGT CTGTTCCTCC AGTGTTTGTG 
    GTCCGACCCA GGAATCAGGT GGCGGGTATG GGCCGCACCG TCACCTTTCA GTGTGAAGCC 
    ACAGGAAGTC CTCAGCCTGC AATATTCTGG CAGAAAGAAG GCAGTCAGAA TCTCCTGTTC 
    TCGTCTCAGC CTCCTCCTCC GTCCAGCAGA TTCTCTGTGT CTCAGACTGG AGATCTGACC 
    ATCACAGCAG TTGAGCGTTC AGATGGAGGA TATTATAGCT GTCAGGCTCT CAACATCGCT 
    GGCAGTGTGA TCACCAAAGC CCTGCTGGAG GTTACAGACG TGATTTCAGA CAGGCCTCCA 
    CCAATCGTCC GTCAGGGTCC AACCAACCAG ACTGTGGCAG TGGACGGGAC GGTGGTTCTG 
    AGCTGCCAAG CCACCGGGAC ACCGATTCCT ACCATCTTAT GGAGGAAAGA TGGAGTTCTG 
    GTGTCCACAC ATGATTCCCG ACTCAAGCAG CTGGACACTG GAGCCCTGCA GATTCGATAC 
    GCAAAGCTGG GCGACACTGG CATGTACACC TGCACGGCTT CCACCCCGAG CGGAGAGGCG 
    TCCTGGAAGG CCTACCTGGA GGTGCAAGAG TTTGGTGCTC CAGTTCAACC GGGCCGTCCC 
    ACAGATCCCA ACCTCATCCC CAGCGCCCCA TCTAAACCAG ACGTCACGGA TGTCAGCCGC 
    ACCTCCGTGT CTCTTTCCTG GAAGCCGAAT CTGAACGCTG GAGCCACACC CACCTCATAC 
    GTCATTGAGG CCTTCAGCCA TGCAGCAGGC AGCAGCTGGC AGACGCTCGC CGATCATGTG 
    AAGACGGAAA GCTTCGTGCT TAAGGGTCTG AAGCCAAGTG CCGTCTACCT GTTTCTGGTG 
    AGAGCGGCCA ATGCGTACGG CCTGAGCGAC CCCAGTCCCA TCAGCGACGC TGTCAAAACA 
    CAAGACGTCC CACCAACCAG CCAGGGAGTG GAGCATGGCC AGATCCAGAG AGAACTGGGA 
    GATGTCGTCA TTCACCTTCA CAATCCCACA ATTCTCTCAT CGTCTTCCGT CAGAGTCCAG 
    TGGACTGTGG AGCAGCAGTC TCAGTATATT CAGGGCTATA AGGTGATGTA CCGTCCGTCT 
    CCAGAAGGCG CTCCGCAGCG AGTGGATTGG GCCATGTTTG AGGTCGGAGC CCCGGGGGAG 
    CACAGCGCTG TCGTCACCCA GCTCAAGAAG GGCATCACCT ATGAGTTCAA AGTGCGCCCT 
    TTCTTCAATG AGTTCCAGGG CACCGACAGC GACATCAAAG TGGGAAAGAC CCTGGAGGAG 
    GCTCCCAGCG CTGCTCCCAG AGGCGTCACC GTAACAGGAA GTGGAGACAA CGGCACAGCA 
    GTCCTGGTCG CCTGGCAACC GCCGCCAGAG GAGGAGCAGA ACGGCGTTGT GCAGGAGTAC 
    AAGATCTGGT GTCTTGGTAA TGAAAGCCGA TACCACATCA ACCGCACAGT GGATGGTTCT 
    ACACACTCGG TGTTGATTCC TGGTCTGGTG GCAGGAGTCA CATACAGGCT AGAGGTGGCA 
    GCAGGCACTG GGGCCGGACC CGGGGTCAAG AGCGACCCTA CGTCCTTCCA GCTTGACTCT 
    TCTGGCCGTG TTCTCCCGCC GCCGCTGGAC GACGATAACA CCATTTCCCA GCAGATCTCA 
    GACGTGGTGC GTCAGCCGGC TTTCATCGCG GGCATCGGCG CCGCCTGCTG GATCATCCTG 
    ATGGTGTTCA GCATCTGGCT ATACCGCCAC CGCAAGAAGA GGAACGGCCT CAGCAGCAGC 
    TACGCCGGCA TCCGCAAAGT GCCCTCCTTC ACCTTCACCC CCACAGTGGC CTATCAGAGA 
    GGCGGAGAGG CCGTCAGCAG TGCAGGAAGG CCGGGTTTGT TGAATATAGG TGAACCAGCT 
    ACACAGCCCT GGCTTGCGGA CACCTGGCCC AACAGCTGCT CCAATCACAA TGACTGCACT 
    ATTAACTGCT GCACCGGCAG CAATGGAAAC AGCGACAGCA ACCTGGCAAC TTACAGCCGC 
    CCAGCCGACT GCATCGCCAA CTACAGCAAC CAGATGGACA ACAAGGCCAC CAACCTGCTG 
    GCTCCAGATA CTGGGCTTTA CAGCGACGTT GACCTTTCAA ACAAGATCAA CGAGATGAAG 
    ACCTTCAACA GCCCCAACCT GAAGGACGGG CGTTTTATGG GGCCTGGTGG TCAACCCACA 
    CCCTACGCCA CCACTCAGCT CATTCAATCC AGCCTGGCCA ACAACAACAT GAACAGCAGC 
    AGTGGCGGAA GCGGAGGAGG AAGCGCTGAC TTTAATGAGA AGCAGAGCTG GAAGAGCAGC 
    CAGGGAACAC CTCAGAAACA GGCCGACATC AACACACCAC TGCAGTATAA CGTCATGGAA 
    CAAAACAAAC TCAACAAGGA CCACTACCGA GGTGAGGGTC CCATCTCAGT CACCATCCCT 
    TATAACCAGG CCCAGGACGT CCTCACAGGA GAAACCTACA GCAGCTCGGA CAGAGGGAGC 
    AGCACCTCTG GAAGTCAAGG GCAGAAAAAG GGTGGACGAA CAACTAAACA AGCCAAACAG 
    AATCCAATGA ATTGGACTGA ACTGTTGCCT CCTCCTCCGG TTAATCCGCC GCCATGTCAA 
    GAATACACCC TGGTGATGGA CGACAGTTAC GACACAGACC TGCAGTGCCC TTTGCCGCCG 
    TCTCGGATGT ATCTGCAACC TGACGAGTTG GAAGAGGAGG AGGACGAGGT GGAGCTGGAG 
    CGTGGACCTA CACCTCCTGT ACGGGGAGCT GCTTCTTCAC CTGCTGCCGT GTCCTACAGC 
    CACCAGTCCA CCGCAACCCT AACCCCGTCC CCTCAGGAGG AGATGCATCC CATGCTGCAG 
    GACAACCCTG ATCTACATGA GCGCAGGAGA CTGTTAGTGA GTCCACCTCC TCCACCACGA 
    CCTCTCTCTC CTCCACACAC ATACGGCTAC ATCTCCAGTC CCCTCGGCCT GGACACGGAC 
    GGCCTAGAGG AAGAGGATGA TGATGAAGAA GAGGGTGACG AAACGGACGC AGAGGTAGCT 
    CGAGTCCACC AGCACCACAT GATGCACCCT CACCAGCAGC ACTTGCCCCA GCAGAGGGCT 
    CTCCTGCGGG GTCTGGAACA AACCCCGGCC TCCAGCACCT GTGACCTGGA GAGCTCTGTG 
    ACTGGGTCTA TGATTAACGG ATGGGGTTCG GCTTCAGAAG AGGACAACGC CTCGTCAGGC 
    CGTTCCAGTG CAGTCAGCTC ATCGGATGGA TCGTTCTTTA CCGATGCCGA TTTCGCCCAG 
    GCTGTGGCAG CTGCGGCTGA ATATGCTGGT CTTCGAGTGG CAAAGCATCC TGGGAAAGGA 
    CATCATCAGC TTCCAGTGTC TGGAGCAGCA CGGAAATACC AAAACCTCCC TGCGGGACAC 
    CGTCCAGGCA GCCCAGTGTC CACAGACAGT AACGTTAGCG CAGCCCCGGG TCAGAGGAGG 
    CCCCTGAAGA GGCAAAAACA ACATCCAGCT GCTCAGACAG GCTACCAGCG CCGAGAGGCC 
    TACACAGAGG ATCTCCCCCC TCCTCCGATT CCTCCTCCAG CCATGCTGAA GTCTCCAACA 
    CACCCGGCCA AAACCATCCC CGAGGGTCCA CGGGGGGCCA TGTCGCCAAG ATCCAGCTCT 
    GCGGATGGCA AGGACAGGCG AACGGGTGCT GGCGGGCCCA GGGTCAGGGA CGGTTCAGAC 
    ACTCGGACCA GCTCTTCAGA GCGGAGGGAT GGCCAAGATG TACAGAGGGG TCAGAGAACG 
    GGCAAGAATA GCAAACAAGA TGAAGCCGTG AAGAGCCGCC AGCACCCAGG CTCAGAGGAC 
    ATCCTACCTT ACAGTCGTCC TTCTTTCCCA GCCGTCCACA GTCCTCGAGG AGAACGGGAC 
    CCCAGTTCCT CCAGTTCACT GTCCTCTCGG GGGTCTGGCG GGCGACGGAG AGGTGAAGGG 
    CCACCAGGGG CCCGCAGAAA CCCTGCAGAC ATTGGGCTAA ACACCATAGG AGCCTTCCAG 
    ACTGGAGGGG ACGAGCTTGA GATGGTGGAG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214697.1
    CDS308..5383
    Translation

    Target ORF information:

    RefSeq Version XM_017359208.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359208.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAGGTCA CATGGTCAGG CGCAGAAGTC CTCATCATTT TCACCTTGCT GCAGTTTGTC 
    CATCCAGAGC TTGCTGAGGG TCCTTTGGCT CCAGACACGG GGACTCAGGA TGGGACTAGC 
    AATAGGGCCA CAGAGACCCC CACTGCAGAA CACGTTGACG ACAACTCTCT GGGCTACACA 
    GGATCCCGTC TGCGGCAGGA AGATTACGCA CCCCGTATTG TTGAACACCC CTCTGATCTG 
    ATCGTATCCA AAGGCGAACC GGCCACCCTA AACTGCAAGG CTGAAGGACG TCCCACGCCC 
    ACAGTGGAGT GGTACAAAGA TGGAGAACGA GTTGAAACCG ACCGGGACAA TCCTCGATCC 
    CACCGCATGC TCCTGCCCAC CGGATCTCTC TTCTTTCTCC GCATTGTTCA CGGCCGGCGC 
    AGCAAACCCG ACGAAGGCTC CTACGTTTGT GTGGCACGTA ACTATCTGGG AGAAGCCGTC 
    AGCCACAACG CCTCGCTGGA AGTAGCTATT CTCCGAGATG ATTTCCGACA GAACCCTGTG 
    GACGTGATGG TGGCAGCGGG GGAGCCAGCT GTAATGGAGT GCCAGCCGCC CCGAGGACAC 
    CCAGAGCCCA CCATCTCCTG GAAGAAAGAT GGGGTCAATA TAGACGACAG AGACGAGAGG 
    ATAACGATAC GAGGAGGAAA GCTCATGATC ACCAACACCA GAAAAACGGA CGCGGGGAAG 
    TACGTGTGCG TGGGAACCAA CATGATGGGG GAACGAGAGA GCGAGATCGC GGAGTTGACT 
    GTTCTCGAGA GGCCCAGTTT CTTGCGAAAG CCGAGCAGCC AGTCCGTCCT TGTGGATCAG 
    AGCGTTGAGT TCAGATGTGA AGCTCAAGGA GACCCCGTGC CCACCATCCG ATGGAGGAAA 
    GAGGACGGAG AACTGCCCAA GGGAAGGTAT GAGATCTGGG AGGACCACAC TCTCAAACTG 
    AAGAGGCTGA CTTCAGGAGA CGCCGGTACT TACACCTGTC AGGCGGAGAA CATGATGGGC 
    AAGACGGAGG CCAGCGCCAC CCTCACCGTC CACGTTGTGT CTGTTCCTCC AGTGTTTGTG 
    GTCCGACCCA GGAATCAGGT GGCGGGTATG GGCCGCACCG TCACCTTTCA GTGTGAAGCC 
    ACAGGAAGTC CTCAGCCTGC AATATTCTGG CAGAAAGAAG GCAGTCAGAA TCTCCTGTTC 
    TCGTCTCAGC CTCCTCCTCC GTCCAGCAGA TTCTCTGTGT CTCAGACTGG AGATCTGACC 
    ATCACAGCAG TTGAGCGTTC AGATGGAGGA TATTATAGCT GTCAGGCTCT CAACATCGCT 
    GGCAGTGTGA TCACCAAAGC CCTGCTGGAG GTTACAGACG TGATTTCAGA CAGGCCTCCA 
    CCAATCGTCC GTCAGGGTCC AACCAACCAG ACTGTGGCAG TGGACGGGAC GGTGGTTCTG 
    AGCTGCCAAG CCACCGGGAC ACCGATTCCT ACCATCTTAT GGAGGAAAGA TGGAGTTCTG 
    GTGTCCACAC ATGATTCCCG ACTCAAGCAG CTGGACACTG GAGCCCTGCA GATTCGATAC 
    GCAAAGCTGG GCGACACTGG CATGTACACC TGCACGGCTT CCACCCCGAG CGGAGAGGCG 
    TCCTGGAAGG CCTACCTGGA GGTGCAAGAG TTTGGTGCTC CAGTTCAACC GGGCCGTCCC 
    ACAGATCCCA ACCTCATCCC CAGCGCCCCA TCTAAACCAG ACGTCACGGA TGTCAGCCGC 
    ACCTCCGTGT CTCTTTCCTG GAAGCCGAAT CTGAACGCTG GAGCCACACC CACCTCATAC 
    GTCATTGAGG CCTTCAGCCA TGCAGCAGGC AGCAGCTGGC AGACGCTCGC CGATCATGTG 
    AAGACGGAAA GCTTCGTGCT TAAGGGTCTG AAGCCAAGTG CCGTCTACCT GTTTCTGGTG 
    AGAGCGGCCA ATGCGTACGG CCTGAGCGAC CCCAGTCCCA TCAGCGACGC TGTCAAAACA 
    CAAGACGTCC CACCAACCAG CCAGGGAGTG GAGCATGGCC AGATCCAGAG AGAACTGGGA 
    GATGTCGTCA TTCACCTTCA CAATCCCACA ATTCTCTCAT CGTCTTCCGT CAGAGTCCAG 
    TGGACTGTGG AGCAGCAGTC TCAGTATATT CAGGGCTATA AGGTGATGTA CCGTCCGTCT 
    CCAGAAGGCG CTCCGCAGCG AGTGGATTGG GCCATGTTTG AGGTCGGAGC CCCGGGGGAG 
    CACAGCGCTG TCGTCACCCA GCTCAAGAAG GGCATCACCT ATGAGTTCAA AGTGCGCCCT 
    TTCTTCAATG AGTTCCAGGG CACCGACAGC GACATCAAAG TGGGAAAGAC CCTGGAGGAG 
    GCTCCCAGCG CTGCTCCCAG AGGCGTCACC GTAACAGGAA GTGGAGACAA CGGCACAGCA 
    GTCCTGGTCG CCTGGCAACC GCCGCCAGAG GAGGAGCAGA ACGGCGTTGT GCAGGAGTAC 
    AAGATCTGGT GTCTTGGTAA TGAAAGCCGA TACCACATCA ACCGCACAGT GGATGGTTCT 
    ACACACTCGG TGTTGATTCC TGGTCTGGTG GCAGGAGTCA CATACAGGCT AGAGGTGGCA 
    GCAGGCACTG GGGCCGGACC CGGGGTCAAG AGCGACCCTA CGTCCTTCCA GCTTGACTCT 
    TCTGGCCGTG TTCTCCCGCC GCCGCTGGAC GACGATAACA CCATTTCCCA GCAGATCTCA 
    GACGTGGTGC GTCAGCCGGC TTTCATCGCG GGCATCGGCG CCGCCTGCTG GATCATCCTG 
    ATGGTGTTCA GCATCTGGCT ATACCGCCAC CGCAAGAAGA GGAACGGCCT CAGCAGCAGC 
    TACGCCGGCA TCCGCAAAGT GCCCTCCTTC ACCTTCACCC CCACAGTGGC CTATCAGAGA 
    GGCGGAGAGG CCGTCAGCAG TGCAGGAAGG CCGGGTTTGT TGAATATAGG TGAACCAGCT 
    ACACAGCCCT GGCTTGCGGA CACCTGGCCC AACAGCTGCT CCAATCACAA TGACTGCACT 
    ATTAACTGCT GCACCGGCAG CAATGGAAAC AGCGACAGCA ACCTGGCAAC TTACAGCCGC 
    CCAGCCGACT GCATCGCCAA CTACAGCAAC CAGATGGACA ACAAGGCCAC CAACCTGCTG 
    GCTCCAGATA CTGGGCTTTA CAGCGACGTT GACCTTTCAA ACAAGATCAA CGAGATGAAG 
    ACCTTCAACA GCCCCAACCT GAAGGACGGG CGTTTTATGG GGCCTGGTGG TCAACCCACA 
    CCCTACGCCA CCACTCAGCT CATTCAATCC AGCCTGGCCA ACAACAACAT GAACAGCAGC 
    AGTGGCGGAA GCGGAGGAGG AAGCGCTGAC TTTAATGAGA AGCAGAGCTG GAAGAGCAGC 
    CAGGGAACAC CTCAGAAACA GGCCGACATC AACACACCAC TGCAGTATAA CGTCATGGAA 
    CAAAACAAAC TCAACAAGGA CCACTACCGA GGTGAGGGTC CCATCTCAGT CACCATCCCT 
    TATAACCAGG CCCAGGACGT CCTCACAGGA GAAACCTACA GCAGCTCGGA CAGAGGGAGC 
    AGCACCTCTG GAAGTCAAGG GCAGAAAAAG GGTGGACGAA CAACTAAACA AGCCAAACAG 
    AATCCAATGA ATTGGACTGA ACTGTTGCCT CCTCCTCCGG TTAATCCGCC GCCATGTCAA 
    GAATACACCC TGGTGATGGA CGACAGTTAC GACACAGACC TGCAGTGCCC TTTGCCGCCG 
    TCTCGGATGT ATCTGCAACC TGACGAGTTG GAAGAGGAGG AGGACGAGGT GGAGCTGGAG 
    CGTGGACCTA CACCTCCTGT ACGGGGAGCT GCTTCTTCAC CTGCTGCCGT GTCCTACAGC 
    CACCAGTCCA CCGCAACCCT AACCCCGTCC CCTCAGGAGG AGATGCATCC CATGCTGCAG 
    GACAACCCTG ATCTACATGA GCGCAGGAGA CTGTTAGTGA GTCCACCTCC TCCACCACGA 
    CCTCTCTCTC CTCCACACAC ATACGGCTAC ATCTCCAGTC CCCTCGGCCT GGACACGGAC 
    GGCCTAGAGG AAGAGGATGA TGATGAAGAA GAGGGTGACG AAACGGACGC AGAGGTAGCT 
    CGAGTCCACC AGCACCACAT GATGCACCCT CACCAGCAGC ACTTGCCCCA GCAGAGGGCT 
    CTCCTGCGGG GTCTGGAACA AACCCCGGCC TCCAGCACCT GTGACCTGGA GAGCTCTGTG 
    ACTGGGTCTA TGATTAACGG ATGGGGTTCG GCTTCAGAAG AGGACAACGC CTCGTCAGGC 
    CGTTCCAGTG CAGTCAGCTC ATCGGATGGA TCGTTCTTTA CCGATGCCGA TTTCGCCCAG 
    GCTGTGGCAG CTGCGGCTGA ATATGCTGGT CTTCGAGTGG CAAAGCATCC TGGGAAAGGA 
    CATCATCAGC TTCCAGTGTC TGGAGCAGCA CGGAAATACC AAAACCTCCC TGCGGGACAC 
    CGTCCAGGCA GCCCAGTGTC CACAGACAGT AACGTTAGCG CAGCCCCGGG TCAGAGGAGG 
    CCCCTGAAGA GGCAAAAACA ACATCCAGCT GCTCAGACAG GCTACCAGCG CCGAGAGGCC 
    TACACAGAGG ATCTCCCCCC TCCTCCGATT CCTCCTCCAG CCATGCTGAA GTCTCCAACA 
    CACCCGGCCA AAACCATCCC CGAGGGTCCA CGGGGGGCCA TGTCGCCAAG ATCCAGCTCT 
    GCGGATGGCA AGGACAGGCG AACGGGTGCT GGCGGGCCCA GGGTCAGGGA CGGTTCAGAC 
    ACTCGGACCA GCTCTTCAGA GCGGAGGGAT GGCCAAGATG TACAGAGGGG TCAGAGAACG 
    GGCAAGAATA GCAAACAAGA TGAAGCCGTG AAGAGCCGCC AGCACCCAGG CTCAGAGGAC 
    ATCCTACCTT ACAGTCGTCC TTCTTTCCCA GCCGTCCACA GTCCTCGAGG AGAACGGGAC 
    CCCAGTTCCT CCAGTTCACT GTCCTCTCGG GGGTCTGGCG GGCGACGGAG AGGTGAAGGG 
    CCACCAGGGG CCCGCAGAAA CCCTGCAGAC ATTGGGCTAA ACACCATAGG AGCCTTCCAG 
    ACTGGAGGGG ACGAGCTTGA GATGGTGGAG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359208.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214697.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa55059
    Clone ID Related Accession (Same CDS sequence) XM_017359208.2 , XM_017359208.1 , NM_001365166.1
    Accession Version XM_017359208.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAGGTCA CATGGTCAGG CGCAGAAGTC CTCATCATTT TCACCTTGCT GCAGTTTGTC 
    CATCCAGAGC TTGCTGAGGG TCCTTTGGCT CCAGACACGG GGACTCAGGA TGGGACTAGC 
    AATAGGGCCA CAGAGACCCC CACTGCAGAA CACGTTGACG ACAACTCTCT GGGCTACACA 
    GGATCCCGTC TGCGGCAGGA AGATTACGCA CCCCGTATTG TTGAACACCC CTCTGATCTG 
    ATCGTATCCA AAGGCGAACC GGCCACCCTA AACTGCAAGG CTGAAGGACG TCCCACGCCC 
    ACAGTGGAGT GGTACAAAGA TGGAGAACGA GTTGAAACCG ACCGGGACAA TCCTCGATCC 
    CACCGCATGC TCCTGCCCAC CGGATCTCTC TTCTTTCTCC GCATTGTTCA CGGCCGGCGC 
    AGCAAACCCG ACGAAGGCTC CTACGTTTGT GTGGCACGTA ACTATCTGGG AGAAGCCGTC 
    AGCCACAACG CCTCGCTGGA AGTAGCTATT CTCCGAGATG ATTTCCGACA GAACCCTGTG 
    GACGTGATGG TGGCAGCGGG GGAGCCAGCT GTAATGGAGT GCCAGCCGCC CCGAGGACAC 
    CCAGAGCCCA CCATCTCCTG GAAGAAAGAT GGGGTCAATA TAGACGACAG AGACGAGAGG 
    ATAACGATAC GAGGAGGAAA GCTCATGATC ACCAACACCA GAAAAACGGA CGCGGGGAAG 
    TACGTGTGCG TGGGAACCAA CATGATGGGG GAACGAGAGA GCGAGATCGC GGAGTTGACT 
    GTTCTCGAGA GGCCCAGTTT CTTGCGAAAG CCGAGCAGCC AGTCCGTCCT TGTGGATCAG 
    AGCGTTGAGT TCAGATGTGA AGCTCAAGGA GACCCCGTGC CCACCATCCG ATGGAGGAAA 
    GAGGACGGAG AACTGCCCAA GGGAAGGTAT GAGATCTGGG AGGACCACAC TCTCAAACTG 
    AAGAGGCTGA CTTCAGGAGA CGCCGGTACT TACACCTGTC AGGCGGAGAA CATGATGGGC 
    AAGACGGAGG CCAGCGCCAC CCTCACCGTC CACGTTGTGT CTGTTCCTCC AGTGTTTGTG 
    GTCCGACCCA GGAATCAGGT GGCGGGTATG GGCCGCACCG TCACCTTTCA GTGTGAAGCC 
    ACAGGAAGTC CTCAGCCTGC AATATTCTGG CAGAAAGAAG GCAGTCAGAA TCTCCTGTTC 
    TCGTCTCAGC CTCCTCCTCC GTCCAGCAGA TTCTCTGTGT CTCAGACTGG AGATCTGACC 
    ATCACAGCAG TTGAGCGTTC AGATGGAGGA TATTATAGCT GTCAGGCTCT CAACATCGCT 
    GGCAGTGTGA TCACCAAAGC CCTGCTGGAG GTTACAGACG TGATTTCAGA CAGGCCTCCA 
    CCAATCGTCC GTCAGGGTCC AACCAACCAG ACTGTGGCAG TGGACGGGAC GGTGGTTCTG 
    AGCTGCCAAG CCACCGGGAC ACCGATTCCT ACCATCTTAT GGAGGAAAGA TGGAGTTCTG 
    GTGTCCACAC ATGATTCCCG ACTCAAGCAG CTGGACACTG GAGCCCTGCA GATTCGATAC 
    GCAAAGCTGG GCGACACTGG CATGTACACC TGCACGGCTT CCACCCCGAG CGGAGAGGCG 
    TCCTGGAAGG CCTACCTGGA GGTGCAAGAG TTTGGTGCTC CAGTTCAACC GGGCCGTCCC 
    ACAGATCCCA ACCTCATCCC CAGCGCCCCA TCTAAACCAG ACGTCACGGA TGTCAGCCGC 
    ACCTCCGTGT CTCTTTCCTG GAAGCCGAAT CTGAACGCTG GAGCCACACC CACCTCATAC 
    GTCATTGAGG CCTTCAGCCA TGCAGCAGGC AGCAGCTGGC AGACGCTCGC CGATCATGTG 
    AAGACGGAAA GCTTCGTGCT TAAGGGTCTG AAGCCAAGTG CCGTCTACCT GTTTCTGGTG 
    AGAGCGGCCA ATGCGTACGG CCTGAGCGAC CCCAGTCCCA TCAGCGACGC TGTCAAAACA 
    CAAGACGTCC CACCAACCAG CCAGGGAGTG GAGCATGGCC AGATCCAGAG AGAACTGGGA 
    GATGTCGTCA TTCACCTTCA CAATCCCACA ATTCTCTCAT CGTCTTCCGT CAGAGTCCAG 
    TGGACTGTGG AGCAGCAGTC TCAGTATATT CAGGGCTATA AGGTGATGTA CCGTCCGTCT 
    CCAGAAGGCG CTCCGCAGCG AGTGGATTGG GCCATGTTTG AGGTCGGAGC CCCGGGGGAG 
    CACAGCGCTG TCGTCACCCA GCTCAAGAAG GGCATCACCT ATGAGTTCAA AGTGCGCCCT 
    TTCTTCAATG AGTTCCAGGG CACCGACAGC GACATCAAAG TGGGAAAGAC CCTGGAGGAG 
    GCTCCCAGCG CTGCTCCCAG AGGCGTCACC GTAACAGGAA GTGGAGACAA CGGCACAGCA 
    GTCCTGGTCG CCTGGCAACC GCCGCCAGAG GAGGAGCAGA ACGGCGTTGT GCAGGAGTAC 
    AAGATCTGGT GTCTTGGTAA TGAAAGCCGA TACCACATCA ACCGCACAGT GGATGGTTCT 
    ACACACTCGG TGTTGATTCC TGGTCTGGTG GCAGGAGTCA CATACAGGCT AGAGGTGGCA 
    GCAGGCACTG GGGCCGGACC CGGGGTCAAG AGCGACCCTA CGTCCTTCCA GCTTGACTCT 
    TCTGGCCGTG TTCTCCCGCC GCCGCTGGAC GACGATAACA CCATTTCCCA GCAGATCTCA 
    GACGTGGTGC GTCAGCCGGC TTTCATCGCG GGCATCGGCG CCGCCTGCTG GATCATCCTG 
    ATGGTGTTCA GCATCTGGCT ATACCGCCAC CGCAAGAAGA GGAACGGCCT CAGCAGCAGC 
    TACGCCGGCA TCCGCAAAGT GCCCTCCTTC ACCTTCACCC CCACAGTGGC CTATCAGAGA 
    GGCGGAGAGG CCGTCAGCAG TGCAGGAAGG CCGGGTTTGT TGAATATAGG TGAACCAGCT 
    ACACAGCCCT GGCTTGCGGA CACCTGGCCC AACAGCTGCT CCAATCACAA TGACTGCACT 
    ATTAACTGCT GCACCGGCAG CAATGGAAAC AGCGACAGCA ACCTGGCAAC TTACAGCCGC 
    CCAGCCGACT GCATCGCCAA CTACAGCAAC CAGATGGACA ACAAGGCCAC CAACCTGCTG 
    GCTCCAGATA CTGGGCTTTA CAGCGACGTT GACCTTTCAA ACAAGATCAA CGAGATGAAG 
    ACCTTCAACA GCCCCAACCT GAAGGACGGG CGTTTTATGG GGCCTGGTGG TCAACCCACA 
    CCCTACGCCA CCACTCAGCT CATTCAATCC AGCCTGGCCA ACAACAACAT GAACAGCAGC 
    AGTGGCGGAA GCGGAGGAGG AAGCGCTGAC TTTAATGAGA AGCAGAGCTG GAAGAGCAGC 
    CAGGGAACAC CTCAGAAACA GGCCGACATC AACACACCAC TGCAGTATAA CGTCATGGAA 
    CAAAACAAAC TCAACAAGGA CCACTACCGA GGTGAGGGTC CCATCTCAGT CACCATCCCT 
    TATAACCAGG CCCAGGACGT CCTCACAGGA GAAACCTACA GCAGCTCGGA CAGAGGGAGC 
    AGCACCTCTG GAAGTCAAGG GCAGAAAAAG GGTGGACGAA CAACTAAACA AGCCAAACAG 
    AATCCAATGA ATTGGACTGA ACTGTTGCCT CCTCCTCCGG TTAATCCGCC GCCATGTCAA 
    GAATACACCC TGGTGATGGA CGACAGTTAC GACACAGACC TGCAGTGCCC TTTGCCGCCG 
    TCTCGGATGT ATCTGCAACC TGACGAGTTG GAAGAGGAGG AGGACGAGGT GGAGCTGGAG 
    CGTGGACCTA CACCTCCTGT ACGGGGAGCT GCTTCTTCAC CTGCTGCCGT GTCCTACAGC 
    CACCAGTCCA CCGCAACCCT AACCCCGTCC CCTCAGGAGG AGATGCATCC CATGCTGCAG 
    GACAACCCTG ATCTACATGA GCGCAGGAGA CTGTTAGTGA GTCCACCTCC TCCACCACGA 
    CCTCTCTCTC CTCCACACAC ATACGGCTAC ATCTCCAGTC CCCTCGGCCT GGACACGGAC 
    GGCCTAGAGG AAGAGGATGA TGATGAAGAA GAGGGTGACG AAACGGACGC AGAGGTAGCT 
    CGAGTCCACC AGCACCACAT GATGCACCCT CACCAGCAGC ACTTGCCCCA GCAGAGGGCT 
    CTCCTGCGGG GTCTGGAACA AACCCCGGCC TCCAGCACCT GTGACCTGGA GAGCTCTGTG 
    ACTGGGTCTA TGATTAACGG ATGGGGTTCG GCTTCAGAAG AGGACAACGC CTCGTCAGGC 
    CGTTCCAGTG CAGTCAGCTC ATCGGATGGA TCGTTCTTTA CCGATGCCGA TTTCGCCCAG 
    GCTGTGGCAG CTGCGGCTGA ATATGCTGGT CTTCGAGTGG CAAAGCATCC TGGGAAAGGA 
    CATCATCAGC TTCCAGTGTC TGGAGCAGCA CGGAAATACC AAAACCTCCC TGCGGGACAC 
    CGTCCAGGCA GCCCAGTGTC CACAGACAGT AACGTTAGCG CAGCCCCGGG TCAGAGGAGG 
    CCCCTGAAGA GGCAAAAACA ACATCCAGCT GCTCAGACAG GCTACCAGCG CCGAGAGGCC 
    TACACAGAGG ATCTCCCCCC TCCTCCGATT CCTCCTCCAG CCATGCTGAA GTCTCCAACA 
    CACCCGGCCA AAACCATCCC CGAGGGTCCA CGGGGGGCCA TGTCGCCAAG ATCCAGCTCT 
    GCGGATGGCA AGGACAGGCG AACGGGTGCT GGCGGGCCCA GGGTCAGGGA CGGTTCAGAC 
    ACTCGGACCA GCTCTTCAGA GCGGAGGGAT GGCCAAGATG TACAGAGGGG TCAGAGAACG 
    GGCAAGAATA GCAAACAAGA TGAAGCCGTG AAGAGCCGCC AGCACCCAGG CTCAGAGGAC 
    ATCCTACCTT ACAGTCGTCC TTCTTTCCCA GCCGTCCACA GTCCTCGAGG AGAACGGGAC 
    CCCAGTTCCT CCAGTTCACT GTCCTCTCGG GGGTCTGGCG GGCGACGGAG AGGTGAAGGG 
    CCACCAGGGG CCCGCAGAAA CCCTGCAGAC ATTGGGCTAA ACACCATAGG AGCCTTCCAG 
    ACTGGAGGGG ACGAGCTTGA GATGGTGGAG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214697.1
    CDS321..5396
    Translation

    Target ORF information:

    RefSeq Version XM_017359208.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359208.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAGGTCA CATGGTCAGG CGCAGAAGTC CTCATCATTT TCACCTTGCT GCAGTTTGTC 
    CATCCAGAGC TTGCTGAGGG TCCTTTGGCT CCAGACACGG GGACTCAGGA TGGGACTAGC 
    AATAGGGCCA CAGAGACCCC CACTGCAGAA CACGTTGACG ACAACTCTCT GGGCTACACA 
    GGATCCCGTC TGCGGCAGGA AGATTACGCA CCCCGTATTG TTGAACACCC CTCTGATCTG 
    ATCGTATCCA AAGGCGAACC GGCCACCCTA AACTGCAAGG CTGAAGGACG TCCCACGCCC 
    ACAGTGGAGT GGTACAAAGA TGGAGAACGA GTTGAAACCG ACCGGGACAA TCCTCGATCC 
    CACCGCATGC TCCTGCCCAC CGGATCTCTC TTCTTTCTCC GCATTGTTCA CGGCCGGCGC 
    AGCAAACCCG ACGAAGGCTC CTACGTTTGT GTGGCACGTA ACTATCTGGG AGAAGCCGTC 
    AGCCACAACG CCTCGCTGGA AGTAGCTATT CTCCGAGATG ATTTCCGACA GAACCCTGTG 
    GACGTGATGG TGGCAGCGGG GGAGCCAGCT GTAATGGAGT GCCAGCCGCC CCGAGGACAC 
    CCAGAGCCCA CCATCTCCTG GAAGAAAGAT GGGGTCAATA TAGACGACAG AGACGAGAGG 
    ATAACGATAC GAGGAGGAAA GCTCATGATC ACCAACACCA GAAAAACGGA CGCGGGGAAG 
    TACGTGTGCG TGGGAACCAA CATGATGGGG GAACGAGAGA GCGAGATCGC GGAGTTGACT 
    GTTCTCGAGA GGCCCAGTTT CTTGCGAAAG CCGAGCAGCC AGTCCGTCCT TGTGGATCAG 
    AGCGTTGAGT TCAGATGTGA AGCTCAAGGA GACCCCGTGC CCACCATCCG ATGGAGGAAA 
    GAGGACGGAG AACTGCCCAA GGGAAGGTAT GAGATCTGGG AGGACCACAC TCTCAAACTG 
    AAGAGGCTGA CTTCAGGAGA CGCCGGTACT TACACCTGTC AGGCGGAGAA CATGATGGGC 
    AAGACGGAGG CCAGCGCCAC CCTCACCGTC CACGTTGTGT CTGTTCCTCC AGTGTTTGTG 
    GTCCGACCCA GGAATCAGGT GGCGGGTATG GGCCGCACCG TCACCTTTCA GTGTGAAGCC 
    ACAGGAAGTC CTCAGCCTGC AATATTCTGG CAGAAAGAAG GCAGTCAGAA TCTCCTGTTC 
    TCGTCTCAGC CTCCTCCTCC GTCCAGCAGA TTCTCTGTGT CTCAGACTGG AGATCTGACC 
    ATCACAGCAG TTGAGCGTTC AGATGGAGGA TATTATAGCT GTCAGGCTCT CAACATCGCT 
    GGCAGTGTGA TCACCAAAGC CCTGCTGGAG GTTACAGACG TGATTTCAGA CAGGCCTCCA 
    CCAATCGTCC GTCAGGGTCC AACCAACCAG ACTGTGGCAG TGGACGGGAC GGTGGTTCTG 
    AGCTGCCAAG CCACCGGGAC ACCGATTCCT ACCATCTTAT GGAGGAAAGA TGGAGTTCTG 
    GTGTCCACAC ATGATTCCCG ACTCAAGCAG CTGGACACTG GAGCCCTGCA GATTCGATAC 
    GCAAAGCTGG GCGACACTGG CATGTACACC TGCACGGCTT CCACCCCGAG CGGAGAGGCG 
    TCCTGGAAGG CCTACCTGGA GGTGCAAGAG TTTGGTGCTC CAGTTCAACC GGGCCGTCCC 
    ACAGATCCCA ACCTCATCCC CAGCGCCCCA TCTAAACCAG ACGTCACGGA TGTCAGCCGC 
    ACCTCCGTGT CTCTTTCCTG GAAGCCGAAT CTGAACGCTG GAGCCACACC CACCTCATAC 
    GTCATTGAGG CCTTCAGCCA TGCAGCAGGC AGCAGCTGGC AGACGCTCGC CGATCATGTG 
    AAGACGGAAA GCTTCGTGCT TAAGGGTCTG AAGCCAAGTG CCGTCTACCT GTTTCTGGTG 
    AGAGCGGCCA ATGCGTACGG CCTGAGCGAC CCCAGTCCCA TCAGCGACGC TGTCAAAACA 
    CAAGACGTCC CACCAACCAG CCAGGGAGTG GAGCATGGCC AGATCCAGAG AGAACTGGGA 
    GATGTCGTCA TTCACCTTCA CAATCCCACA ATTCTCTCAT CGTCTTCCGT CAGAGTCCAG 
    TGGACTGTGG AGCAGCAGTC TCAGTATATT CAGGGCTATA AGGTGATGTA CCGTCCGTCT 
    CCAGAAGGCG CTCCGCAGCG AGTGGATTGG GCCATGTTTG AGGTCGGAGC CCCGGGGGAG 
    CACAGCGCTG TCGTCACCCA GCTCAAGAAG GGCATCACCT ATGAGTTCAA AGTGCGCCCT 
    TTCTTCAATG AGTTCCAGGG CACCGACAGC GACATCAAAG TGGGAAAGAC CCTGGAGGAG 
    GCTCCCAGCG CTGCTCCCAG AGGCGTCACC GTAACAGGAA GTGGAGACAA CGGCACAGCA 
    GTCCTGGTCG CCTGGCAACC GCCGCCAGAG GAGGAGCAGA ACGGCGTTGT GCAGGAGTAC 
    AAGATCTGGT GTCTTGGTAA TGAAAGCCGA TACCACATCA ACCGCACAGT GGATGGTTCT 
    ACACACTCGG TGTTGATTCC TGGTCTGGTG GCAGGAGTCA CATACAGGCT AGAGGTGGCA 
    GCAGGCACTG GGGCCGGACC CGGGGTCAAG AGCGACCCTA CGTCCTTCCA GCTTGACTCT 
    TCTGGCCGTG TTCTCCCGCC GCCGCTGGAC GACGATAACA CCATTTCCCA GCAGATCTCA 
    GACGTGGTGC GTCAGCCGGC TTTCATCGCG GGCATCGGCG CCGCCTGCTG GATCATCCTG 
    ATGGTGTTCA GCATCTGGCT ATACCGCCAC CGCAAGAAGA GGAACGGCCT CAGCAGCAGC 
    TACGCCGGCA TCCGCAAAGT GCCCTCCTTC ACCTTCACCC CCACAGTGGC CTATCAGAGA 
    GGCGGAGAGG CCGTCAGCAG TGCAGGAAGG CCGGGTTTGT TGAATATAGG TGAACCAGCT 
    ACACAGCCCT GGCTTGCGGA CACCTGGCCC AACAGCTGCT CCAATCACAA TGACTGCACT 
    ATTAACTGCT GCACCGGCAG CAATGGAAAC AGCGACAGCA ACCTGGCAAC TTACAGCCGC 
    CCAGCCGACT GCATCGCCAA CTACAGCAAC CAGATGGACA ACAAGGCCAC CAACCTGCTG 
    GCTCCAGATA CTGGGCTTTA CAGCGACGTT GACCTTTCAA ACAAGATCAA CGAGATGAAG 
    ACCTTCAACA GCCCCAACCT GAAGGACGGG CGTTTTATGG GGCCTGGTGG TCAACCCACA 
    CCCTACGCCA CCACTCAGCT CATTCAATCC AGCCTGGCCA ACAACAACAT GAACAGCAGC 
    AGTGGCGGAA GCGGAGGAGG AAGCGCTGAC TTTAATGAGA AGCAGAGCTG GAAGAGCAGC 
    CAGGGAACAC CTCAGAAACA GGCCGACATC AACACACCAC TGCAGTATAA CGTCATGGAA 
    CAAAACAAAC TCAACAAGGA CCACTACCGA GGTGAGGGTC CCATCTCAGT CACCATCCCT 
    TATAACCAGG CCCAGGACGT CCTCACAGGA GAAACCTACA GCAGCTCGGA CAGAGGGAGC 
    AGCACCTCTG GAAGTCAAGG GCAGAAAAAG GGTGGACGAA CAACTAAACA AGCCAAACAG 
    AATCCAATGA ATTGGACTGA ACTGTTGCCT CCTCCTCCGG TTAATCCGCC GCCATGTCAA 
    GAATACACCC TGGTGATGGA CGACAGTTAC GACACAGACC TGCAGTGCCC TTTGCCGCCG 
    TCTCGGATGT ATCTGCAACC TGACGAGTTG GAAGAGGAGG AGGACGAGGT GGAGCTGGAG 
    CGTGGACCTA CACCTCCTGT ACGGGGAGCT GCTTCTTCAC CTGCTGCCGT GTCCTACAGC 
    CACCAGTCCA CCGCAACCCT AACCCCGTCC CCTCAGGAGG AGATGCATCC CATGCTGCAG 
    GACAACCCTG ATCTACATGA GCGCAGGAGA CTGTTAGTGA GTCCACCTCC TCCACCACGA 
    CCTCTCTCTC CTCCACACAC ATACGGCTAC ATCTCCAGTC CCCTCGGCCT GGACACGGAC 
    GGCCTAGAGG AAGAGGATGA TGATGAAGAA GAGGGTGACG AAACGGACGC AGAGGTAGCT 
    CGAGTCCACC AGCACCACAT GATGCACCCT CACCAGCAGC ACTTGCCCCA GCAGAGGGCT 
    CTCCTGCGGG GTCTGGAACA AACCCCGGCC TCCAGCACCT GTGACCTGGA GAGCTCTGTG 
    ACTGGGTCTA TGATTAACGG ATGGGGTTCG GCTTCAGAAG AGGACAACGC CTCGTCAGGC 
    CGTTCCAGTG CAGTCAGCTC ATCGGATGGA TCGTTCTTTA CCGATGCCGA TTTCGCCCAG 
    GCTGTGGCAG CTGCGGCTGA ATATGCTGGT CTTCGAGTGG CAAAGCATCC TGGGAAAGGA 
    CATCATCAGC TTCCAGTGTC TGGAGCAGCA CGGAAATACC AAAACCTCCC TGCGGGACAC 
    CGTCCAGGCA GCCCAGTGTC CACAGACAGT AACGTTAGCG CAGCCCCGGG TCAGAGGAGG 
    CCCCTGAAGA GGCAAAAACA ACATCCAGCT GCTCAGACAG GCTACCAGCG CCGAGAGGCC 
    TACACAGAGG ATCTCCCCCC TCCTCCGATT CCTCCTCCAG CCATGCTGAA GTCTCCAACA 
    CACCCGGCCA AAACCATCCC CGAGGGTCCA CGGGGGGCCA TGTCGCCAAG ATCCAGCTCT 
    GCGGATGGCA AGGACAGGCG AACGGGTGCT GGCGGGCCCA GGGTCAGGGA CGGTTCAGAC 
    ACTCGGACCA GCTCTTCAGA GCGGAGGGAT GGCCAAGATG TACAGAGGGG TCAGAGAACG 
    GGCAAGAATA GCAAACAAGA TGAAGCCGTG AAGAGCCGCC AGCACCCAGG CTCAGAGGAC 
    ATCCTACCTT ACAGTCGTCC TTCTTTCCCA GCCGTCCACA GTCCTCGAGG AGAACGGGAC 
    CCCAGTTCCT CCAGTTCACT GTCCTCTCGG GGGTCTGGCG GGCGACGGAG AGGTGAAGGG 
    CCACCAGGGG CCCGCAGAAA CCCTGCAGAC ATTGGGCTAA ACACCATAGG AGCCTTCCAG 
    ACTGGAGGGG ACGAGCTTGA GATGGTGGAG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359209.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214698.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID ODa55060
    Clone ID Related Accession (Same CDS sequence) XM_017359209.2 , XM_017359209.1
    Accession Version XM_017359209.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTGTGTCTG TTCCTCCAGT GTTTGTGGTC 
    CGACCCAGGA ATCAGGTGGC GGGTATGGGC CGCACCGTCA CCTTTCAGTG TGAAGCCACA 
    GGAAGTCCTC AGCCTGCAAT ATTCTGGCAG AAAGAAGGCA GTCAGAATCT CCTGTTCTCG 
    TCTCAGCCTC CTCCTCCGTC CAGCAGATTC TCTGTGTCTC AGACTGGAGA TCTGACCATC 
    ACAGCAGTTG AGCGTTCAGA TGGAGGATAT TATAGCTGTC AGGCTCTCAA CATCGCTGGC 
    AGTGTGATCA CCAAAGCCCT GCTGGAGGTT ACAGACGTGA TTTCAGACAG GCCTCCACCA 
    ATCGTCCGTC AGGGTCCAAC CAACCAGACT GTGGCAGTGG ACGGGACGGT GGTTCTGAGC 
    TGCCAAGCCA CCGGGACACC GATTCCTACC ATCTTATGGA GGAAAGATGG AGTTCTGGTG 
    TCCACACATG ATTCCCGACT CAAGCAGCTG GACACTGGAG CCCTGCAGAT TCGATACGCA 
    AAGCTGGGCG ACACTGGCAT GTACACCTGC ACGGCTTCCA CCCCGAGCGG AGAGGCGTCC 
    TGGAAGGCCT ACCTGGAGGT GCAAGAGTTT GGTGCTCCAG TTCAACCGGG CCGTCCCACA 
    GATCCCAACC TCATCCCCAG CGCCCCATCT AAACCAGACG TCACGGATGT CAGCCGCACC 
    TCCGTGTCTC TTTCCTGGAA GCCGAATCTG AACGCTGGAG CCACACCCAC CTCATACGTC 
    ATTGAGGCCT TCAGCCATGC AGCAGGCAGC AGCTGGCAGA CGCTCGCCGA TCATGTGAAG 
    ACGGAAAGCT TCGTGCTTAA GGGTCTGAAG CCAAGTGCCG TCTACCTGTT TCTGGTGAGA 
    GCGGCCAATG CGTACGGCCT GAGCGACCCC AGTCCCATCA GCGACGCTGT CAAAACACAA 
    GACGTCCCAC CAACCAGCCA GGGAGTGGAG CATGGCCAGA TCCAGAGAGA ACTGGGAGAT 
    GTCGTCATTC ACCTTCACAA TCCCACAATT CTCTCATCGT CTTCCGTCAG AGTCCAGTGG 
    ACTGTGGAGC AGCAGTCTCA GTATATTCAG GGCTATAAGG TGATGTACCG TCCGTCTCCA 
    GAAGGCGCTC CGCAGCGAGT GGATTGGGCC ATGTTTGAGG TCGGAGCCCC GGGGGAGCAC 
    AGCGCTGTCG TCACCCAGCT CAAGAAGGGC ATCACCTATG AGTTCAAAGT GCGCCCTTTC 
    TTCAATGAGT TCCAGGGCAC CGACAGCGAC ATCAAAGTGG GAAAGACCCT GGAGGAGGCT 
    CCCAGCGCTG CTCCCAGAGG CGTCACCGTA ACAGGAAGTG GAGACAACGG CACAGCAGTC 
    CTGGTCGCCT GGCAACCGCC GCCAGAGGAG GAGCAGAACG GCGTTGTGCA GGAGTACAAG 
    ATCTGGTGTC TTGGTAATGA AAGCCGATAC CACATCAACC GCACAGTGGA TGGTTCTACA 
    CACTCGGTGT TGATTCCTGG TCTGGTGGCA GGAGTCACAT ACAGGCTAGA GGTGGCAGCA 
    GGCACTGGGG CCGGACCCGG GGTCAAGAGC GACCCTACGT CCTTCCAGCT TGACTCTTCT 
    GGCCGTGTTC TCCCGCCGCC GCTGGACGAC GATAACACCA TTTCCCAGCA GATCTCAGAC 
    GTGGTGCGTC AGCCGGCTTT CATCGCGGGC ATCGGCGCCG CCTGCTGGAT CATCCTGATG 
    GTGTTCAGCA TCTGGCTATA CCGCCACCGC AAGAAGAGGA ACGGCCTCAG CAGCAGCTAC 
    GCCGGCATCC GCAAAGTGCC CTCCTTCACC TTCACCCCCA CAGTGGCCTA TCAGAGAGGC 
    GGAGAGGCCG TCAGCAGTGC AGGAAGGCCG GGTTTGTTGA ATATAGGTGA ACCAGCTACA 
    CAGCCCTGGC TTGCGGACAC CTGGCCCAAC AGCTGCTCCA ATCACAATGA CTGCACTATT 
    AACTGCTGCA CCGGCAGCAA TGGAAACAGC GACAGCAACC TGGCAACTTA CAGCCGCCCA 
    GCCGACTGCA TCGCCAACTA CAGCAACCAG ATGGACAACA AGGCCACCAA CCTGCTGGCT 
    CCAGATACTG GGCTTTACAG CGACGTTGAC CTTTCAAACA AGATCAACGA GATGAAGACC 
    TTCAACAGCC CCAACCTGAA GGACGGGCGT TTTATGGGGC CTGGTGGTCA ACCCACACCC 
    TACGCCACCA CTCAGCTCAT TCAATCCAGC CTGGCCAACA ACAACATGAA CAGCAGCAGT 
    GGCGGAAGCG GAGGAGGAAG CGCTGACTTT AATGAGAAGC AGAGCTGGAA GAGCAGCCAG 
    GGAACACCTC AGAAACAGGC CGACATCAAC ACACCACTGC AGTATAACGT CATGGAACAA 
    AACAAACTCA ACAAGGACCA CTACCGAGGT GAGGGTCCCA TCTCAGTCAC CATCCCTTAT 
    AACCAGGCCC AGGACGTCCT CACAGGAGAA ACCTACAGCA GCTCGGACAG AGGGAGCAGC 
    ACCTCTGGAA GTCAAGGGCA GAAAAAGGGT GGACGAACAA CTAAACAAGC CAAACAGAAT 
    CCAATGAATT GGACTGAACT GTTGCCTCCT CCTCCGGTTA ATCCGCCGCC ATGTCAAGAA 
    TACACCCTGG TGATGGACGA CAGTTACGAC ACAGACCTGC AGTGCCCTTT GCCGCCGTCT 
    CGGATGTATC TGCAACCTGA CGAGTTGGAA GAGGAGGAGG ACGAGGTGGA GCTGGAGCGT 
    GGACCTACAC CTCCTGTACG GGGAGCTGCT TCTTCACCTG CTGCCGTGTC CTACAGCCAC 
    CAGTCCACCG CAACCCTAAC CCCGTCCCCT CAGGAGGAGA TGCATCCCAT GCTGCAGGAC 
    AACCCTGATC TACATGAGCG CAGGAGACTG TTAGTGAGTC CACCTCCTCC ACCACGACCT 
    CTCTCTCCTC CACACACATA CGGCTACATC TCCAGTCCCC TCGGCCTGGA CACGGACGGC 
    CTAGAGGAAG AGGATGATGA TGAAGAAGAG GGTGACGAAA CGGACGCAGA GGTAGCTCGA 
    GTCCACCAGC ACCACATGAT GCACCCTCAC CAGCAGCACT TGCCCCAGCA GAGGGCTCTC 
    CTGCGGGGTC TGGAACAAAC CCCGGCCTCC AGCACCTGTG ACCTGGAGAG CTCTGTGACT 
    GGGTCTATGA TTAACGGATG GGGTTCGGCT TCAGAAGAGG ACAACGCCTC GTCAGGCCGT 
    TCCAGTGCAG TCAGCTCATC GGATGGATCG TTCTTTACCG ATGCCGATTT CGCCCAGGCT 
    GTGGCAGCTG CGGCTGAATA TGCTGGTCTT CGAGTGGCAA AGCATCCTGG GAAAGGACAT 
    CATCAGCTTC CAGTGTCTGG AGCAGCACGG AAATACCAAA ACCTCCCTGC GGGACACCGT 
    CCAGGCAGCC CAGTGTCCAC AGACAGTAAC GTTAGCGCAG CCCCGGGTCA GAGGAGGCCC 
    CTGAAGAGGC AAAAACAACA TCCAGCTGCT CAGACAGGCT ACCAGCGCCG AGAGGCCTAC 
    ACAGAGGATC TCCCCCCTCC TCCGATTCCT CCTCCAGCCA TGCTGAAGTC TCCAACACAC 
    CCGGCCAAAA CCATCCCCGA GGGTCCACGG GGGGCCATGT CGCCAAGATC CAGCTCTGCG 
    GATGGCAAGG ACAGGCGAAC GGGTGCTGGC GGGCCCAGGG TCAGGGACGG TTCAGACACT 
    CGGACCAGCT CTTCAGAGCG GAGGGATGGC CAAGATGTAC AGAGGGGTCA GAGAACGGGC 
    AAGAATAGCA AACAAGATGA AGCCGTGAAG AGCCGCCAGC ACCCAGGCTC AGAGGACATC 
    CTACCTTACA GTCGTCCTTC TTTCCCAGCC GTCCACAGTC CTCGAGGAGA ACGGGACCCC 
    AGTTCCTCCA GTTCACTGTC CTCTCGGGGG TCTGGCGGGC GACGGAGAGG TGAAGGGCCA 
    CCAGGGGCCC GCAGAAACCC TGCAGACATT GGGCTAAACA CCATAGGAGC CTTCCAGACT 
    GGAGGGGACG AGCTTGAGAT GGTGGAGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214698.1
    CDS767..5719
    Translation

    Target ORF information:

    RefSeq Version XM_017359209.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359209.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTGTGTCTG TTCCTCCAGT GTTTGTGGTC 
    CGACCCAGGA ATCAGGTGGC GGGTATGGGC CGCACCGTCA CCTTTCAGTG TGAAGCCACA 
    GGAAGTCCTC AGCCTGCAAT ATTCTGGCAG AAAGAAGGCA GTCAGAATCT CCTGTTCTCG 
    TCTCAGCCTC CTCCTCCGTC CAGCAGATTC TCTGTGTCTC AGACTGGAGA TCTGACCATC 
    ACAGCAGTTG AGCGTTCAGA TGGAGGATAT TATAGCTGTC AGGCTCTCAA CATCGCTGGC 
    AGTGTGATCA CCAAAGCCCT GCTGGAGGTT ACAGACGTGA TTTCAGACAG GCCTCCACCA 
    ATCGTCCGTC AGGGTCCAAC CAACCAGACT GTGGCAGTGG ACGGGACGGT GGTTCTGAGC 
    TGCCAAGCCA CCGGGACACC GATTCCTACC ATCTTATGGA GGAAAGATGG AGTTCTGGTG 
    TCCACACATG ATTCCCGACT CAAGCAGCTG GACACTGGAG CCCTGCAGAT TCGATACGCA 
    AAGCTGGGCG ACACTGGCAT GTACACCTGC ACGGCTTCCA CCCCGAGCGG AGAGGCGTCC 
    TGGAAGGCCT ACCTGGAGGT GCAAGAGTTT GGTGCTCCAG TTCAACCGGG CCGTCCCACA 
    GATCCCAACC TCATCCCCAG CGCCCCATCT AAACCAGACG TCACGGATGT CAGCCGCACC 
    TCCGTGTCTC TTTCCTGGAA GCCGAATCTG AACGCTGGAG CCACACCCAC CTCATACGTC 
    ATTGAGGCCT TCAGCCATGC AGCAGGCAGC AGCTGGCAGA CGCTCGCCGA TCATGTGAAG 
    ACGGAAAGCT TCGTGCTTAA GGGTCTGAAG CCAAGTGCCG TCTACCTGTT TCTGGTGAGA 
    GCGGCCAATG CGTACGGCCT GAGCGACCCC AGTCCCATCA GCGACGCTGT CAAAACACAA 
    GACGTCCCAC CAACCAGCCA GGGAGTGGAG CATGGCCAGA TCCAGAGAGA ACTGGGAGAT 
    GTCGTCATTC ACCTTCACAA TCCCACAATT CTCTCATCGT CTTCCGTCAG AGTCCAGTGG 
    ACTGTGGAGC AGCAGTCTCA GTATATTCAG GGCTATAAGG TGATGTACCG TCCGTCTCCA 
    GAAGGCGCTC CGCAGCGAGT GGATTGGGCC ATGTTTGAGG TCGGAGCCCC GGGGGAGCAC 
    AGCGCTGTCG TCACCCAGCT CAAGAAGGGC ATCACCTATG AGTTCAAAGT GCGCCCTTTC 
    TTCAATGAGT TCCAGGGCAC CGACAGCGAC ATCAAAGTGG GAAAGACCCT GGAGGAGGCT 
    CCCAGCGCTG CTCCCAGAGG CGTCACCGTA ACAGGAAGTG GAGACAACGG CACAGCAGTC 
    CTGGTCGCCT GGCAACCGCC GCCAGAGGAG GAGCAGAACG GCGTTGTGCA GGAGTACAAG 
    ATCTGGTGTC TTGGTAATGA AAGCCGATAC CACATCAACC GCACAGTGGA TGGTTCTACA 
    CACTCGGTGT TGATTCCTGG TCTGGTGGCA GGAGTCACAT ACAGGCTAGA GGTGGCAGCA 
    GGCACTGGGG CCGGACCCGG GGTCAAGAGC GACCCTACGT CCTTCCAGCT TGACTCTTCT 
    GGCCGTGTTC TCCCGCCGCC GCTGGACGAC GATAACACCA TTTCCCAGCA GATCTCAGAC 
    GTGGTGCGTC AGCCGGCTTT CATCGCGGGC ATCGGCGCCG CCTGCTGGAT CATCCTGATG 
    GTGTTCAGCA TCTGGCTATA CCGCCACCGC AAGAAGAGGA ACGGCCTCAG CAGCAGCTAC 
    GCCGGCATCC GCAAAGTGCC CTCCTTCACC TTCACCCCCA CAGTGGCCTA TCAGAGAGGC 
    GGAGAGGCCG TCAGCAGTGC AGGAAGGCCG GGTTTGTTGA ATATAGGTGA ACCAGCTACA 
    CAGCCCTGGC TTGCGGACAC CTGGCCCAAC AGCTGCTCCA ATCACAATGA CTGCACTATT 
    AACTGCTGCA CCGGCAGCAA TGGAAACAGC GACAGCAACC TGGCAACTTA CAGCCGCCCA 
    GCCGACTGCA TCGCCAACTA CAGCAACCAG ATGGACAACA AGGCCACCAA CCTGCTGGCT 
    CCAGATACTG GGCTTTACAG CGACGTTGAC CTTTCAAACA AGATCAACGA GATGAAGACC 
    TTCAACAGCC CCAACCTGAA GGACGGGCGT TTTATGGGGC CTGGTGGTCA ACCCACACCC 
    TACGCCACCA CTCAGCTCAT TCAATCCAGC CTGGCCAACA ACAACATGAA CAGCAGCAGT 
    GGCGGAAGCG GAGGAGGAAG CGCTGACTTT AATGAGAAGC AGAGCTGGAA GAGCAGCCAG 
    GGAACACCTC AGAAACAGGC CGACATCAAC ACACCACTGC AGTATAACGT CATGGAACAA 
    AACAAACTCA ACAAGGACCA CTACCGAGGT GAGGGTCCCA TCTCAGTCAC CATCCCTTAT 
    AACCAGGCCC AGGACGTCCT CACAGGAGAA ACCTACAGCA GCTCGGACAG AGGGAGCAGC 
    ACCTCTGGAA GTCAAGGGCA GAAAAAGGGT GGACGAACAA CTAAACAAGC CAAACAGAAT 
    CCAATGAATT GGACTGAACT GTTGCCTCCT CCTCCGGTTA ATCCGCCGCC ATGTCAAGAA 
    TACACCCTGG TGATGGACGA CAGTTACGAC ACAGACCTGC AGTGCCCTTT GCCGCCGTCT 
    CGGATGTATC TGCAACCTGA CGAGTTGGAA GAGGAGGAGG ACGAGGTGGA GCTGGAGCGT 
    GGACCTACAC CTCCTGTACG GGGAGCTGCT TCTTCACCTG CTGCCGTGTC CTACAGCCAC 
    CAGTCCACCG CAACCCTAAC CCCGTCCCCT CAGGAGGAGA TGCATCCCAT GCTGCAGGAC 
    AACCCTGATC TACATGAGCG CAGGAGACTG TTAGTGAGTC CACCTCCTCC ACCACGACCT 
    CTCTCTCCTC CACACACATA CGGCTACATC TCCAGTCCCC TCGGCCTGGA CACGGACGGC 
    CTAGAGGAAG AGGATGATGA TGAAGAAGAG GGTGACGAAA CGGACGCAGA GGTAGCTCGA 
    GTCCACCAGC ACCACATGAT GCACCCTCAC CAGCAGCACT TGCCCCAGCA GAGGGCTCTC 
    CTGCGGGGTC TGGAACAAAC CCCGGCCTCC AGCACCTGTG ACCTGGAGAG CTCTGTGACT 
    GGGTCTATGA TTAACGGATG GGGTTCGGCT TCAGAAGAGG ACAACGCCTC GTCAGGCCGT 
    TCCAGTGCAG TCAGCTCATC GGATGGATCG TTCTTTACCG ATGCCGATTT CGCCCAGGCT 
    GTGGCAGCTG CGGCTGAATA TGCTGGTCTT CGAGTGGCAA AGCATCCTGG GAAAGGACAT 
    CATCAGCTTC CAGTGTCTGG AGCAGCACGG AAATACCAAA ACCTCCCTGC GGGACACCGT 
    CCAGGCAGCC CAGTGTCCAC AGACAGTAAC GTTAGCGCAG CCCCGGGTCA GAGGAGGCCC 
    CTGAAGAGGC AAAAACAACA TCCAGCTGCT CAGACAGGCT ACCAGCGCCG AGAGGCCTAC 
    ACAGAGGATC TCCCCCCTCC TCCGATTCCT CCTCCAGCCA TGCTGAAGTC TCCAACACAC 
    CCGGCCAAAA CCATCCCCGA GGGTCCACGG GGGGCCATGT CGCCAAGATC CAGCTCTGCG 
    GATGGCAAGG ACAGGCGAAC GGGTGCTGGC GGGCCCAGGG TCAGGGACGG TTCAGACACT 
    CGGACCAGCT CTTCAGAGCG GAGGGATGGC CAAGATGTAC AGAGGGGTCA GAGAACGGGC 
    AAGAATAGCA AACAAGATGA AGCCGTGAAG AGCCGCCAGC ACCCAGGCTC AGAGGACATC 
    CTACCTTACA GTCGTCCTTC TTTCCCAGCC GTCCACAGTC CTCGAGGAGA ACGGGACCCC 
    AGTTCCTCCA GTTCACTGTC CTCTCGGGGG TCTGGCGGGC GACGGAGAGG TGAAGGGCCA 
    CCAGGGGCCC GCAGAAACCC TGCAGACATT GGGCTAAACA CCATAGGAGC CTTCCAGACT 
    GGAGGGGACG AGCTTGAGAT GGTGGAGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359209.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214698.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa55060
    Clone ID Related Accession (Same CDS sequence) XM_017359209.2 , XM_017359209.1
    Accession Version XM_017359209.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTGTGTCTG TTCCTCCAGT GTTTGTGGTC 
    CGACCCAGGA ATCAGGTGGC GGGTATGGGC CGCACCGTCA CCTTTCAGTG TGAAGCCACA 
    GGAAGTCCTC AGCCTGCAAT ATTCTGGCAG AAAGAAGGCA GTCAGAATCT CCTGTTCTCG 
    TCTCAGCCTC CTCCTCCGTC CAGCAGATTC TCTGTGTCTC AGACTGGAGA TCTGACCATC 
    ACAGCAGTTG AGCGTTCAGA TGGAGGATAT TATAGCTGTC AGGCTCTCAA CATCGCTGGC 
    AGTGTGATCA CCAAAGCCCT GCTGGAGGTT ACAGACGTGA TTTCAGACAG GCCTCCACCA 
    ATCGTCCGTC AGGGTCCAAC CAACCAGACT GTGGCAGTGG ACGGGACGGT GGTTCTGAGC 
    TGCCAAGCCA CCGGGACACC GATTCCTACC ATCTTATGGA GGAAAGATGG AGTTCTGGTG 
    TCCACACATG ATTCCCGACT CAAGCAGCTG GACACTGGAG CCCTGCAGAT TCGATACGCA 
    AAGCTGGGCG ACACTGGCAT GTACACCTGC ACGGCTTCCA CCCCGAGCGG AGAGGCGTCC 
    TGGAAGGCCT ACCTGGAGGT GCAAGAGTTT GGTGCTCCAG TTCAACCGGG CCGTCCCACA 
    GATCCCAACC TCATCCCCAG CGCCCCATCT AAACCAGACG TCACGGATGT CAGCCGCACC 
    TCCGTGTCTC TTTCCTGGAA GCCGAATCTG AACGCTGGAG CCACACCCAC CTCATACGTC 
    ATTGAGGCCT TCAGCCATGC AGCAGGCAGC AGCTGGCAGA CGCTCGCCGA TCATGTGAAG 
    ACGGAAAGCT TCGTGCTTAA GGGTCTGAAG CCAAGTGCCG TCTACCTGTT TCTGGTGAGA 
    GCGGCCAATG CGTACGGCCT GAGCGACCCC AGTCCCATCA GCGACGCTGT CAAAACACAA 
    GACGTCCCAC CAACCAGCCA GGGAGTGGAG CATGGCCAGA TCCAGAGAGA ACTGGGAGAT 
    GTCGTCATTC ACCTTCACAA TCCCACAATT CTCTCATCGT CTTCCGTCAG AGTCCAGTGG 
    ACTGTGGAGC AGCAGTCTCA GTATATTCAG GGCTATAAGG TGATGTACCG TCCGTCTCCA 
    GAAGGCGCTC CGCAGCGAGT GGATTGGGCC ATGTTTGAGG TCGGAGCCCC GGGGGAGCAC 
    AGCGCTGTCG TCACCCAGCT CAAGAAGGGC ATCACCTATG AGTTCAAAGT GCGCCCTTTC 
    TTCAATGAGT TCCAGGGCAC CGACAGCGAC ATCAAAGTGG GAAAGACCCT GGAGGAGGCT 
    CCCAGCGCTG CTCCCAGAGG CGTCACCGTA ACAGGAAGTG GAGACAACGG CACAGCAGTC 
    CTGGTCGCCT GGCAACCGCC GCCAGAGGAG GAGCAGAACG GCGTTGTGCA GGAGTACAAG 
    ATCTGGTGTC TTGGTAATGA AAGCCGATAC CACATCAACC GCACAGTGGA TGGTTCTACA 
    CACTCGGTGT TGATTCCTGG TCTGGTGGCA GGAGTCACAT ACAGGCTAGA GGTGGCAGCA 
    GGCACTGGGG CCGGACCCGG GGTCAAGAGC GACCCTACGT CCTTCCAGCT TGACTCTTCT 
    GGCCGTGTTC TCCCGCCGCC GCTGGACGAC GATAACACCA TTTCCCAGCA GATCTCAGAC 
    GTGGTGCGTC AGCCGGCTTT CATCGCGGGC ATCGGCGCCG CCTGCTGGAT CATCCTGATG 
    GTGTTCAGCA TCTGGCTATA CCGCCACCGC AAGAAGAGGA ACGGCCTCAG CAGCAGCTAC 
    GCCGGCATCC GCAAAGTGCC CTCCTTCACC TTCACCCCCA CAGTGGCCTA TCAGAGAGGC 
    GGAGAGGCCG TCAGCAGTGC AGGAAGGCCG GGTTTGTTGA ATATAGGTGA ACCAGCTACA 
    CAGCCCTGGC TTGCGGACAC CTGGCCCAAC AGCTGCTCCA ATCACAATGA CTGCACTATT 
    AACTGCTGCA CCGGCAGCAA TGGAAACAGC GACAGCAACC TGGCAACTTA CAGCCGCCCA 
    GCCGACTGCA TCGCCAACTA CAGCAACCAG ATGGACAACA AGGCCACCAA CCTGCTGGCT 
    CCAGATACTG GGCTTTACAG CGACGTTGAC CTTTCAAACA AGATCAACGA GATGAAGACC 
    TTCAACAGCC CCAACCTGAA GGACGGGCGT TTTATGGGGC CTGGTGGTCA ACCCACACCC 
    TACGCCACCA CTCAGCTCAT TCAATCCAGC CTGGCCAACA ACAACATGAA CAGCAGCAGT 
    GGCGGAAGCG GAGGAGGAAG CGCTGACTTT AATGAGAAGC AGAGCTGGAA GAGCAGCCAG 
    GGAACACCTC AGAAACAGGC CGACATCAAC ACACCACTGC AGTATAACGT CATGGAACAA 
    AACAAACTCA ACAAGGACCA CTACCGAGGT GAGGGTCCCA TCTCAGTCAC CATCCCTTAT 
    AACCAGGCCC AGGACGTCCT CACAGGAGAA ACCTACAGCA GCTCGGACAG AGGGAGCAGC 
    ACCTCTGGAA GTCAAGGGCA GAAAAAGGGT GGACGAACAA CTAAACAAGC CAAACAGAAT 
    CCAATGAATT GGACTGAACT GTTGCCTCCT CCTCCGGTTA ATCCGCCGCC ATGTCAAGAA 
    TACACCCTGG TGATGGACGA CAGTTACGAC ACAGACCTGC AGTGCCCTTT GCCGCCGTCT 
    CGGATGTATC TGCAACCTGA CGAGTTGGAA GAGGAGGAGG ACGAGGTGGA GCTGGAGCGT 
    GGACCTACAC CTCCTGTACG GGGAGCTGCT TCTTCACCTG CTGCCGTGTC CTACAGCCAC 
    CAGTCCACCG CAACCCTAAC CCCGTCCCCT CAGGAGGAGA TGCATCCCAT GCTGCAGGAC 
    AACCCTGATC TACATGAGCG CAGGAGACTG TTAGTGAGTC CACCTCCTCC ACCACGACCT 
    CTCTCTCCTC CACACACATA CGGCTACATC TCCAGTCCCC TCGGCCTGGA CACGGACGGC 
    CTAGAGGAAG AGGATGATGA TGAAGAAGAG GGTGACGAAA CGGACGCAGA GGTAGCTCGA 
    GTCCACCAGC ACCACATGAT GCACCCTCAC CAGCAGCACT TGCCCCAGCA GAGGGCTCTC 
    CTGCGGGGTC TGGAACAAAC CCCGGCCTCC AGCACCTGTG ACCTGGAGAG CTCTGTGACT 
    GGGTCTATGA TTAACGGATG GGGTTCGGCT TCAGAAGAGG ACAACGCCTC GTCAGGCCGT 
    TCCAGTGCAG TCAGCTCATC GGATGGATCG TTCTTTACCG ATGCCGATTT CGCCCAGGCT 
    GTGGCAGCTG CGGCTGAATA TGCTGGTCTT CGAGTGGCAA AGCATCCTGG GAAAGGACAT 
    CATCAGCTTC CAGTGTCTGG AGCAGCACGG AAATACCAAA ACCTCCCTGC GGGACACCGT 
    CCAGGCAGCC CAGTGTCCAC AGACAGTAAC GTTAGCGCAG CCCCGGGTCA GAGGAGGCCC 
    CTGAAGAGGC AAAAACAACA TCCAGCTGCT CAGACAGGCT ACCAGCGCCG AGAGGCCTAC 
    ACAGAGGATC TCCCCCCTCC TCCGATTCCT CCTCCAGCCA TGCTGAAGTC TCCAACACAC 
    CCGGCCAAAA CCATCCCCGA GGGTCCACGG GGGGCCATGT CGCCAAGATC CAGCTCTGCG 
    GATGGCAAGG ACAGGCGAAC GGGTGCTGGC GGGCCCAGGG TCAGGGACGG TTCAGACACT 
    CGGACCAGCT CTTCAGAGCG GAGGGATGGC CAAGATGTAC AGAGGGGTCA GAGAACGGGC 
    AAGAATAGCA AACAAGATGA AGCCGTGAAG AGCCGCCAGC ACCCAGGCTC AGAGGACATC 
    CTACCTTACA GTCGTCCTTC TTTCCCAGCC GTCCACAGTC CTCGAGGAGA ACGGGACCCC 
    AGTTCCTCCA GTTCACTGTC CTCTCGGGGG TCTGGCGGGC GACGGAGAGG TGAAGGGCCA 
    CCAGGGGCCC GCAGAAACCC TGCAGACATT GGGCTAAACA CCATAGGAGC CTTCCAGACT 
    GGAGGGGACG AGCTTGAGAT GGTGGAGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214698.1
    CDS941..5893
    Translation

    Target ORF information:

    RefSeq Version XM_017359209.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359209.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTGTGTCTG TTCCTCCAGT GTTTGTGGTC 
    CGACCCAGGA ATCAGGTGGC GGGTATGGGC CGCACCGTCA CCTTTCAGTG TGAAGCCACA 
    GGAAGTCCTC AGCCTGCAAT ATTCTGGCAG AAAGAAGGCA GTCAGAATCT CCTGTTCTCG 
    TCTCAGCCTC CTCCTCCGTC CAGCAGATTC TCTGTGTCTC AGACTGGAGA TCTGACCATC 
    ACAGCAGTTG AGCGTTCAGA TGGAGGATAT TATAGCTGTC AGGCTCTCAA CATCGCTGGC 
    AGTGTGATCA CCAAAGCCCT GCTGGAGGTT ACAGACGTGA TTTCAGACAG GCCTCCACCA 
    ATCGTCCGTC AGGGTCCAAC CAACCAGACT GTGGCAGTGG ACGGGACGGT GGTTCTGAGC 
    TGCCAAGCCA CCGGGACACC GATTCCTACC ATCTTATGGA GGAAAGATGG AGTTCTGGTG 
    TCCACACATG ATTCCCGACT CAAGCAGCTG GACACTGGAG CCCTGCAGAT TCGATACGCA 
    AAGCTGGGCG ACACTGGCAT GTACACCTGC ACGGCTTCCA CCCCGAGCGG AGAGGCGTCC 
    TGGAAGGCCT ACCTGGAGGT GCAAGAGTTT GGTGCTCCAG TTCAACCGGG CCGTCCCACA 
    GATCCCAACC TCATCCCCAG CGCCCCATCT AAACCAGACG TCACGGATGT CAGCCGCACC 
    TCCGTGTCTC TTTCCTGGAA GCCGAATCTG AACGCTGGAG CCACACCCAC CTCATACGTC 
    ATTGAGGCCT TCAGCCATGC AGCAGGCAGC AGCTGGCAGA CGCTCGCCGA TCATGTGAAG 
    ACGGAAAGCT TCGTGCTTAA GGGTCTGAAG CCAAGTGCCG TCTACCTGTT TCTGGTGAGA 
    GCGGCCAATG CGTACGGCCT GAGCGACCCC AGTCCCATCA GCGACGCTGT CAAAACACAA 
    GACGTCCCAC CAACCAGCCA GGGAGTGGAG CATGGCCAGA TCCAGAGAGA ACTGGGAGAT 
    GTCGTCATTC ACCTTCACAA TCCCACAATT CTCTCATCGT CTTCCGTCAG AGTCCAGTGG 
    ACTGTGGAGC AGCAGTCTCA GTATATTCAG GGCTATAAGG TGATGTACCG TCCGTCTCCA 
    GAAGGCGCTC CGCAGCGAGT GGATTGGGCC ATGTTTGAGG TCGGAGCCCC GGGGGAGCAC 
    AGCGCTGTCG TCACCCAGCT CAAGAAGGGC ATCACCTATG AGTTCAAAGT GCGCCCTTTC 
    TTCAATGAGT TCCAGGGCAC CGACAGCGAC ATCAAAGTGG GAAAGACCCT GGAGGAGGCT 
    CCCAGCGCTG CTCCCAGAGG CGTCACCGTA ACAGGAAGTG GAGACAACGG CACAGCAGTC 
    CTGGTCGCCT GGCAACCGCC GCCAGAGGAG GAGCAGAACG GCGTTGTGCA GGAGTACAAG 
    ATCTGGTGTC TTGGTAATGA AAGCCGATAC CACATCAACC GCACAGTGGA TGGTTCTACA 
    CACTCGGTGT TGATTCCTGG TCTGGTGGCA GGAGTCACAT ACAGGCTAGA GGTGGCAGCA 
    GGCACTGGGG CCGGACCCGG GGTCAAGAGC GACCCTACGT CCTTCCAGCT TGACTCTTCT 
    GGCCGTGTTC TCCCGCCGCC GCTGGACGAC GATAACACCA TTTCCCAGCA GATCTCAGAC 
    GTGGTGCGTC AGCCGGCTTT CATCGCGGGC ATCGGCGCCG CCTGCTGGAT CATCCTGATG 
    GTGTTCAGCA TCTGGCTATA CCGCCACCGC AAGAAGAGGA ACGGCCTCAG CAGCAGCTAC 
    GCCGGCATCC GCAAAGTGCC CTCCTTCACC TTCACCCCCA CAGTGGCCTA TCAGAGAGGC 
    GGAGAGGCCG TCAGCAGTGC AGGAAGGCCG GGTTTGTTGA ATATAGGTGA ACCAGCTACA 
    CAGCCCTGGC TTGCGGACAC CTGGCCCAAC AGCTGCTCCA ATCACAATGA CTGCACTATT 
    AACTGCTGCA CCGGCAGCAA TGGAAACAGC GACAGCAACC TGGCAACTTA CAGCCGCCCA 
    GCCGACTGCA TCGCCAACTA CAGCAACCAG ATGGACAACA AGGCCACCAA CCTGCTGGCT 
    CCAGATACTG GGCTTTACAG CGACGTTGAC CTTTCAAACA AGATCAACGA GATGAAGACC 
    TTCAACAGCC CCAACCTGAA GGACGGGCGT TTTATGGGGC CTGGTGGTCA ACCCACACCC 
    TACGCCACCA CTCAGCTCAT TCAATCCAGC CTGGCCAACA ACAACATGAA CAGCAGCAGT 
    GGCGGAAGCG GAGGAGGAAG CGCTGACTTT AATGAGAAGC AGAGCTGGAA GAGCAGCCAG 
    GGAACACCTC AGAAACAGGC CGACATCAAC ACACCACTGC AGTATAACGT CATGGAACAA 
    AACAAACTCA ACAAGGACCA CTACCGAGGT GAGGGTCCCA TCTCAGTCAC CATCCCTTAT 
    AACCAGGCCC AGGACGTCCT CACAGGAGAA ACCTACAGCA GCTCGGACAG AGGGAGCAGC 
    ACCTCTGGAA GTCAAGGGCA GAAAAAGGGT GGACGAACAA CTAAACAAGC CAAACAGAAT 
    CCAATGAATT GGACTGAACT GTTGCCTCCT CCTCCGGTTA ATCCGCCGCC ATGTCAAGAA 
    TACACCCTGG TGATGGACGA CAGTTACGAC ACAGACCTGC AGTGCCCTTT GCCGCCGTCT 
    CGGATGTATC TGCAACCTGA CGAGTTGGAA GAGGAGGAGG ACGAGGTGGA GCTGGAGCGT 
    GGACCTACAC CTCCTGTACG GGGAGCTGCT TCTTCACCTG CTGCCGTGTC CTACAGCCAC 
    CAGTCCACCG CAACCCTAAC CCCGTCCCCT CAGGAGGAGA TGCATCCCAT GCTGCAGGAC 
    AACCCTGATC TACATGAGCG CAGGAGACTG TTAGTGAGTC CACCTCCTCC ACCACGACCT 
    CTCTCTCCTC CACACACATA CGGCTACATC TCCAGTCCCC TCGGCCTGGA CACGGACGGC 
    CTAGAGGAAG AGGATGATGA TGAAGAAGAG GGTGACGAAA CGGACGCAGA GGTAGCTCGA 
    GTCCACCAGC ACCACATGAT GCACCCTCAC CAGCAGCACT TGCCCCAGCA GAGGGCTCTC 
    CTGCGGGGTC TGGAACAAAC CCCGGCCTCC AGCACCTGTG ACCTGGAGAG CTCTGTGACT 
    GGGTCTATGA TTAACGGATG GGGTTCGGCT TCAGAAGAGG ACAACGCCTC GTCAGGCCGT 
    TCCAGTGCAG TCAGCTCATC GGATGGATCG TTCTTTACCG ATGCCGATTT CGCCCAGGCT 
    GTGGCAGCTG CGGCTGAATA TGCTGGTCTT CGAGTGGCAA AGCATCCTGG GAAAGGACAT 
    CATCAGCTTC CAGTGTCTGG AGCAGCACGG AAATACCAAA ACCTCCCTGC GGGACACCGT 
    CCAGGCAGCC CAGTGTCCAC AGACAGTAAC GTTAGCGCAG CCCCGGGTCA GAGGAGGCCC 
    CTGAAGAGGC AAAAACAACA TCCAGCTGCT CAGACAGGCT ACCAGCGCCG AGAGGCCTAC 
    ACAGAGGATC TCCCCCCTCC TCCGATTCCT CCTCCAGCCA TGCTGAAGTC TCCAACACAC 
    CCGGCCAAAA CCATCCCCGA GGGTCCACGG GGGGCCATGT CGCCAAGATC CAGCTCTGCG 
    GATGGCAAGG ACAGGCGAAC GGGTGCTGGC GGGCCCAGGG TCAGGGACGG TTCAGACACT 
    CGGACCAGCT CTTCAGAGCG GAGGGATGGC CAAGATGTAC AGAGGGGTCA GAGAACGGGC 
    AAGAATAGCA AACAAGATGA AGCCGTGAAG AGCCGCCAGC ACCCAGGCTC AGAGGACATC 
    CTACCTTACA GTCGTCCTTC TTTCCCAGCC GTCCACAGTC CTCGAGGAGA ACGGGACCCC 
    AGTTCCTCCA GTTCACTGTC CTCTCGGGGG TCTGGCGGGC GACGGAGAGG TGAAGGGCCA 
    CCAGGGGCCC GCAGAAACCC TGCAGACATT GGGCTAAACA CCATAGGAGC CTTCCAGACT 
    GGAGGGGACG AGCTTGAGAT GGTGGAGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359210.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214699.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID ODa55061
    Clone ID Related Accession (Same CDS sequence) XM_017359210.1 , XM_017359210.2
    Accession Version XM_017359210.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTGTGTCTG TTCCTCCAGT GTTTGTGGTC 
    CGACCCAGGA ATCAGGTGGC GGGTATGGGC CGCACCGTCA CCTTTCAGTG TGAAGCCACA 
    GGAAGTCCTC AGCCTGCAAT ATTCTGGCAG AAAGAAGGCA GTCAGAATCT CCTGTTCTCG 
    TCTCAGCCTC CTCCTCCGTC CAGCAGATTC TCTGTGTCTC AGACTGGAGA TCTGACCATC 
    ACAGCAGTTG AGCGTTCAGA TGGAGGATAT TATAGCTGTC AGGCTCTCAA CATCGCTGGC 
    AGTGTGATCA CCAAAGCCCT GCTGGAGGTT ACAGACGTGA TTTCAGACAG GCCTCCACCA 
    ATCGTCCGTC AGGGTCCAAC CAACCAGACT GTGGCAGTGG ACGGGACGGT GGTTCTGAGC 
    TGCCAAGCCA CCGGGACACC GATTCCTACC ATCTTATGGA GGAAAGATGG AGTTCTGGTG 
    TCCACACATG ATTCCCGACT CAAGCAGCTG GACACTGGAG CCCTGCAGAT TCGATACGCA 
    AAGCTGGGCG ACACTGGCAT GTACACCTGC ACGGCTTCCA CCCCGAGCGG AGAGGCGTCC 
    TGGAAGGCCT ACCTGGAGGT GCAAGAGTTT GGTGCTCCAG TTCAACCGGG CCGTCCCACA 
    GATCCCAACC TCATCCCCAG CGCCCCATCT AAACCAGACG TCACGGATGT CAGCCGCACC 
    TCCGTGTCTC TTTCCTGGAA GCCGAATCTG AACGCTGGAG CCACACCCAC CTCATACGTC 
    ATTGAGGCCT TCAGCCATGC AGCAGGCAGC AGCTGGCAGA CGCTCGCCGA TCATGTGAAG 
    ACGGAAAGCT TCGTGCTTAA GGGTCTGAAG CCAAGTGCCG TCTACCTGTT TCTGGTGAGA 
    GCGGCCAATG CGTACGGCCT GAGCGACCCC AGTCCCATCA GCGACGCTGT CAAAACACAA 
    GACGTCCCAC CAACCAGCCA GGGAGTGGAG CATGGCCAGA TCCAGAGAGA ACTGGGAGAT 
    GTCGTCATTC ACCTTCACAA TCCCACAATT CTCTCATCGT CTTCCGTCAG AGTCCAGTGG 
    ACTGTGGAGC AGCAGTCTCA GTATATTCAG GGCTATAAGG TGATGTACCG TCCGTCTCCA 
    GAAGGCGCTC CGCAGCGAGT GGATTGGGCC ATGTTTGAGG TCGGAGCCCC GGGGGAGCAC 
    AGCGCTGTCG TCACCCAGCT CAAGAAGGGC ATCACCTATG AGTTCAAAGT GCGCCCTTTC 
    TTCAATGAGT TCCAGGGCAC CGACAGCGAC ATCAAAGTGG GAAAGACCCT GGAGGAGGCT 
    CCCAGCGCTG CTCCCAGAGG CGTCACCGTA ACAGGAAGTG GAGACAACGG CACAGCAGTC 
    CTGGTCGCCT GGCAACCGCC GCCAGAGGAG GAGCAGAACG GCGTTGTGCA GGAGTACAAG 
    ATCTGGTGTC TTGGTAATGA AAGCCGATAC CACATCAACC GCACAGTGGA TGGTTCTACA 
    CACTCGGTGT TGATTCCTGG TCTGGTGGCA GGAGTCACAT ACAGGCTAGA GGTGGCAGCA 
    GGCACTGGGG CCGGACCCGG GGTCAAGAGC GACCCTACGT CCTTCCAGCT TGACTCTTCT 
    GGCCGTGTTC TCCCGCCGCC GCTGGACGAC GATAACACCA TTTCCCAGCA GATCTCAGAC 
    GTGGTGCGTC AGCCGGCTTT CATCGCGGGC ATCGGCGCCG CCTGCTGGAT CATCCTGATG 
    GTGTTCAGCA TCTGGCTATA CCGCCACCGC AAGAAGAGGA ACGGCCTCAG CAGCAGCTAC 
    GCCGGCATCC GCAAAGTGCC CTCCTTCACC TTCACCCCCA CAGTGGCCTA TCAGAGAGGC 
    GGAGAGGCCG TCAGCAGTGC AGGAAGGCCG GGTTTGTTGA ATATAGGTGA ACCAGCTACA 
    CAGCCCTGGC TTGCGGACAC CTGGCCCAAC AGCTGCTCCA ATCACAATGA CTGCACTATT 
    AACTGCTGCA CCGGCAGCAA TGGAAACAGC GACAGCAACC TGGCAACTTA CAGCCGCCCA 
    GCCGACTGCA TCGCCAACTA CAGCAACCAG ATGGACAACA AGGCCACCAA CCTGCTGGCT 
    CCAGATACTG GGCTTTACAG CGACGTTGAC CTTTCAAACA AGATCAACGA GATGAAGACC 
    TTCAACAGCC CCAACCTGAA GGACGGGCGT TTTATGGGGC CTGGTGGTCA ACCCACACCC 
    TACGCCACCA CTCAGCTCAT TCAATCCAGC CTGGCCAACA ACAACATGAA CAGCAGCAGT 
    GGCGGAAGCG GAGGAGGAAG CGCTGACTTT AATGAGAAGC AGAGCTGGAA GAGCAGCCAG 
    GGAACACCTC AGAAACAGGC CGACATCAAC ACACCACTGC AGTATAACGT CATGGAACAA 
    AACAAACTCA ACAAGGACCA CTACCGAGGT GAGGGTCCCA TCTCAGTCAC CATCCCTTAT 
    AACCAGGCCC AGGACGTCCT CACAGGAGAA ACCTACAGCA GCTCGGACAG AGGGAGCAGC 
    ACCTCTGGAA GTCAAGGGCA GAAAAAGGGT GGACGAACAA CTAAACAAGC CAAACAGAAT 
    CCAATGAATT GGACTGAACT GTTGCCTCCT CCTCCGGTTA ATCCGCCGCC ATGTCAAGAA 
    TACACCCTGG TGATGGACGA CAGTTACGAC ACAGACCTGC AGTGCCCTTT GCCGCCGTCT 
    CGGATGTATC TGCAACCTGA CGAGTTGGAA GAGGAGGAGG ACGAGGTGGA GCTGGAGCGT 
    GGACCTACAC CTCCTGTACG GGGAGCTGCT TCTTCACCTG CTGCCGTGTC CTACAGCCAC 
    CAGTCCACCG CAACCCTAAC CCCGTCCCCT CAGGAGGAGA TGCATCCCAT GCTGCAGGAC 
    AACCCTGATC TACATGAGCG CAGGAGACTG TTAGTGAGTC CACCTCCTCC ACCACGACCT 
    CTCTCTCCTC CACACACATA CGGCTACATC TCCAGTCCCC TCGGCCTGGA CACGGACGGC 
    CTAGAGGAAG AGGATGATGA TGAAGAAGAG GGTGACGAAA CGGACGCAGA GGTAGCTCGA 
    GTCCACCAGC ACCACATGAT GCACCCTCAC CAGCAGCACT TGCCCCAGCA GAGGGCTCTC 
    CTGCGGGGTC TGGAACAAAC CCCGGCCTCC AGCACCTGTG ACCTGGAGAG CTCTGTGACT 
    GGGTCTATGA TTAACGGATG GGGTTCGGCT TCAGAAGAGG ACAACGCCTC GTCAGGCCGT 
    TCCAGTGCAG TCAGCTCATC GGATGGATCG TTCTTTACCG ATGCCGATTT CGCCCAGGCT 
    GTGGCAGCTG CGGCTGAATA TGCTGGTCTT CGAGTGGCAA AGCATCCTGG GAAAGGACAT 
    CATCAGCTTC CAGTGTCTGG AGCACGGAAA TACCAAAACC TCCCTGCGGG ACACCGTCCA 
    GGCAGCCCAG TGTCCACAGA CAGTAACGTT AGCGCAGCCC CGGGTCAGAG GAGGCCCCTG 
    AAGAGGCAAA AACAACATCC AGCTGCTCAG ACAGGCTACC AGCGCCGAGA GGCCTACACA 
    GAGGATCTCC CCCCTCCTCC GATTCCTCCT CCAGCCATGC TGAAGTCTCC AACACACCCG 
    GCCAAAACCA TCCCCGAGGG TCCACGGGGG GCCATGTCGC CAAGATCCAG CTCTGCGGAT 
    GGCAAGGACA GGCGAACGGG TGCTGGCGGG CCCAGGGTCA GGGACGGTTC AGACACTCGG 
    ACCAGCTCTT CAGAGCGGAG GGATGGCCAA GATGTACAGA GGGGTCAGAG AACGGGCAAG 
    AATAGCAAAC AAGATGAAGC CGTGAAGAGC CGCCAGCACC CAGGCTCAGA GGACATCCTA 
    CCTTACAGTC GTCCTTCTTT CCCAGCCGTC CACAGTCCTC GAGGAGAACG GGACCCCAGT 
    TCCTCCAGTT CACTGTCCTC TCGGGGGTCT GGCGGGCGAC GGAGAGGTGA AGGGCCACCA 
    GGGGCCCGCA GAAACCCTGC AGACATTGGG CTAAACACCA TAGGAGCCTT CCAGACTGGA 
    GGGGACGAGC TTGAGATGGT GGAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214699.1
    CDS324..5273
    Translation

    Target ORF information:

    RefSeq Version XM_017359210.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359210.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTGTGTCTG TTCCTCCAGT GTTTGTGGTC 
    CGACCCAGGA ATCAGGTGGC GGGTATGGGC CGCACCGTCA CCTTTCAGTG TGAAGCCACA 
    GGAAGTCCTC AGCCTGCAAT ATTCTGGCAG AAAGAAGGCA GTCAGAATCT CCTGTTCTCG 
    TCTCAGCCTC CTCCTCCGTC CAGCAGATTC TCTGTGTCTC AGACTGGAGA TCTGACCATC 
    ACAGCAGTTG AGCGTTCAGA TGGAGGATAT TATAGCTGTC AGGCTCTCAA CATCGCTGGC 
    AGTGTGATCA CCAAAGCCCT GCTGGAGGTT ACAGACGTGA TTTCAGACAG GCCTCCACCA 
    ATCGTCCGTC AGGGTCCAAC CAACCAGACT GTGGCAGTGG ACGGGACGGT GGTTCTGAGC 
    TGCCAAGCCA CCGGGACACC GATTCCTACC ATCTTATGGA GGAAAGATGG AGTTCTGGTG 
    TCCACACATG ATTCCCGACT CAAGCAGCTG GACACTGGAG CCCTGCAGAT TCGATACGCA 
    AAGCTGGGCG ACACTGGCAT GTACACCTGC ACGGCTTCCA CCCCGAGCGG AGAGGCGTCC 
    TGGAAGGCCT ACCTGGAGGT GCAAGAGTTT GGTGCTCCAG TTCAACCGGG CCGTCCCACA 
    GATCCCAACC TCATCCCCAG CGCCCCATCT AAACCAGACG TCACGGATGT CAGCCGCACC 
    TCCGTGTCTC TTTCCTGGAA GCCGAATCTG AACGCTGGAG CCACACCCAC CTCATACGTC 
    ATTGAGGCCT TCAGCCATGC AGCAGGCAGC AGCTGGCAGA CGCTCGCCGA TCATGTGAAG 
    ACGGAAAGCT TCGTGCTTAA GGGTCTGAAG CCAAGTGCCG TCTACCTGTT TCTGGTGAGA 
    GCGGCCAATG CGTACGGCCT GAGCGACCCC AGTCCCATCA GCGACGCTGT CAAAACACAA 
    GACGTCCCAC CAACCAGCCA GGGAGTGGAG CATGGCCAGA TCCAGAGAGA ACTGGGAGAT 
    GTCGTCATTC ACCTTCACAA TCCCACAATT CTCTCATCGT CTTCCGTCAG AGTCCAGTGG 
    ACTGTGGAGC AGCAGTCTCA GTATATTCAG GGCTATAAGG TGATGTACCG TCCGTCTCCA 
    GAAGGCGCTC CGCAGCGAGT GGATTGGGCC ATGTTTGAGG TCGGAGCCCC GGGGGAGCAC 
    AGCGCTGTCG TCACCCAGCT CAAGAAGGGC ATCACCTATG AGTTCAAAGT GCGCCCTTTC 
    TTCAATGAGT TCCAGGGCAC CGACAGCGAC ATCAAAGTGG GAAAGACCCT GGAGGAGGCT 
    CCCAGCGCTG CTCCCAGAGG CGTCACCGTA ACAGGAAGTG GAGACAACGG CACAGCAGTC 
    CTGGTCGCCT GGCAACCGCC GCCAGAGGAG GAGCAGAACG GCGTTGTGCA GGAGTACAAG 
    ATCTGGTGTC TTGGTAATGA AAGCCGATAC CACATCAACC GCACAGTGGA TGGTTCTACA 
    CACTCGGTGT TGATTCCTGG TCTGGTGGCA GGAGTCACAT ACAGGCTAGA GGTGGCAGCA 
    GGCACTGGGG CCGGACCCGG GGTCAAGAGC GACCCTACGT CCTTCCAGCT TGACTCTTCT 
    GGCCGTGTTC TCCCGCCGCC GCTGGACGAC GATAACACCA TTTCCCAGCA GATCTCAGAC 
    GTGGTGCGTC AGCCGGCTTT CATCGCGGGC ATCGGCGCCG CCTGCTGGAT CATCCTGATG 
    GTGTTCAGCA TCTGGCTATA CCGCCACCGC AAGAAGAGGA ACGGCCTCAG CAGCAGCTAC 
    GCCGGCATCC GCAAAGTGCC CTCCTTCACC TTCACCCCCA CAGTGGCCTA TCAGAGAGGC 
    GGAGAGGCCG TCAGCAGTGC AGGAAGGCCG GGTTTGTTGA ATATAGGTGA ACCAGCTACA 
    CAGCCCTGGC TTGCGGACAC CTGGCCCAAC AGCTGCTCCA ATCACAATGA CTGCACTATT 
    AACTGCTGCA CCGGCAGCAA TGGAAACAGC GACAGCAACC TGGCAACTTA CAGCCGCCCA 
    GCCGACTGCA TCGCCAACTA CAGCAACCAG ATGGACAACA AGGCCACCAA CCTGCTGGCT 
    CCAGATACTG GGCTTTACAG CGACGTTGAC CTTTCAAACA AGATCAACGA GATGAAGACC 
    TTCAACAGCC CCAACCTGAA GGACGGGCGT TTTATGGGGC CTGGTGGTCA ACCCACACCC 
    TACGCCACCA CTCAGCTCAT TCAATCCAGC CTGGCCAACA ACAACATGAA CAGCAGCAGT 
    GGCGGAAGCG GAGGAGGAAG CGCTGACTTT AATGAGAAGC AGAGCTGGAA GAGCAGCCAG 
    GGAACACCTC AGAAACAGGC CGACATCAAC ACACCACTGC AGTATAACGT CATGGAACAA 
    AACAAACTCA ACAAGGACCA CTACCGAGGT GAGGGTCCCA TCTCAGTCAC CATCCCTTAT 
    AACCAGGCCC AGGACGTCCT CACAGGAGAA ACCTACAGCA GCTCGGACAG AGGGAGCAGC 
    ACCTCTGGAA GTCAAGGGCA GAAAAAGGGT GGACGAACAA CTAAACAAGC CAAACAGAAT 
    CCAATGAATT GGACTGAACT GTTGCCTCCT CCTCCGGTTA ATCCGCCGCC ATGTCAAGAA 
    TACACCCTGG TGATGGACGA CAGTTACGAC ACAGACCTGC AGTGCCCTTT GCCGCCGTCT 
    CGGATGTATC TGCAACCTGA CGAGTTGGAA GAGGAGGAGG ACGAGGTGGA GCTGGAGCGT 
    GGACCTACAC CTCCTGTACG GGGAGCTGCT TCTTCACCTG CTGCCGTGTC CTACAGCCAC 
    CAGTCCACCG CAACCCTAAC CCCGTCCCCT CAGGAGGAGA TGCATCCCAT GCTGCAGGAC 
    AACCCTGATC TACATGAGCG CAGGAGACTG TTAGTGAGTC CACCTCCTCC ACCACGACCT 
    CTCTCTCCTC CACACACATA CGGCTACATC TCCAGTCCCC TCGGCCTGGA CACGGACGGC 
    CTAGAGGAAG AGGATGATGA TGAAGAAGAG GGTGACGAAA CGGACGCAGA GGTAGCTCGA 
    GTCCACCAGC ACCACATGAT GCACCCTCAC CAGCAGCACT TGCCCCAGCA GAGGGCTCTC 
    CTGCGGGGTC TGGAACAAAC CCCGGCCTCC AGCACCTGTG ACCTGGAGAG CTCTGTGACT 
    GGGTCTATGA TTAACGGATG GGGTTCGGCT TCAGAAGAGG ACAACGCCTC GTCAGGCCGT 
    TCCAGTGCAG TCAGCTCATC GGATGGATCG TTCTTTACCG ATGCCGATTT CGCCCAGGCT 
    GTGGCAGCTG CGGCTGAATA TGCTGGTCTT CGAGTGGCAA AGCATCCTGG GAAAGGACAT 
    CATCAGCTTC CAGTGTCTGG AGCACGGAAA TACCAAAACC TCCCTGCGGG ACACCGTCCA 
    GGCAGCCCAG TGTCCACAGA CAGTAACGTT AGCGCAGCCC CGGGTCAGAG GAGGCCCCTG 
    AAGAGGCAAA AACAACATCC AGCTGCTCAG ACAGGCTACC AGCGCCGAGA GGCCTACACA 
    GAGGATCTCC CCCCTCCTCC GATTCCTCCT CCAGCCATGC TGAAGTCTCC AACACACCCG 
    GCCAAAACCA TCCCCGAGGG TCCACGGGGG GCCATGTCGC CAAGATCCAG CTCTGCGGAT 
    GGCAAGGACA GGCGAACGGG TGCTGGCGGG CCCAGGGTCA GGGACGGTTC AGACACTCGG 
    ACCAGCTCTT CAGAGCGGAG GGATGGCCAA GATGTACAGA GGGGTCAGAG AACGGGCAAG 
    AATAGCAAAC AAGATGAAGC CGTGAAGAGC CGCCAGCACC CAGGCTCAGA GGACATCCTA 
    CCTTACAGTC GTCCTTCTTT CCCAGCCGTC CACAGTCCTC GAGGAGAACG GGACCCCAGT 
    TCCTCCAGTT CACTGTCCTC TCGGGGGTCT GGCGGGCGAC GGAGAGGTGA AGGGCCACCA 
    GGGGCCCGCA GAAACCCTGC AGACATTGGG CTAAACACCA TAGGAGCCTT CCAGACTGGA 
    GGGGACGAGC TTGAGATGGT GGAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359210.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214699.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID ODa55061
    Clone ID Related Accession (Same CDS sequence) XM_017359210.1 , XM_017359210.2
    Accession Version XM_017359210.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359210.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTGTGTCTG TTCCTCCAGT GTTTGTGGTC 
    CGACCCAGGA ATCAGGTGGC GGGTATGGGC CGCACCGTCA CCTTTCAGTG TGAAGCCACA 
    GGAAGTCCTC AGCCTGCAAT ATTCTGGCAG AAAGAAGGCA GTCAGAATCT CCTGTTCTCG 
    TCTCAGCCTC CTCCTCCGTC CAGCAGATTC TCTGTGTCTC AGACTGGAGA TCTGACCATC 
    ACAGCAGTTG AGCGTTCAGA TGGAGGATAT TATAGCTGTC AGGCTCTCAA CATCGCTGGC 
    AGTGTGATCA CCAAAGCCCT GCTGGAGGTT ACAGACGTGA TTTCAGACAG GCCTCCACCA 
    ATCGTCCGTC AGGGTCCAAC CAACCAGACT GTGGCAGTGG ACGGGACGGT GGTTCTGAGC 
    TGCCAAGCCA CCGGGACACC GATTCCTACC ATCTTATGGA GGAAAGATGG AGTTCTGGTG 
    TCCACACATG ATTCCCGACT CAAGCAGCTG GACACTGGAG CCCTGCAGAT TCGATACGCA 
    AAGCTGGGCG ACACTGGCAT GTACACCTGC ACGGCTTCCA CCCCGAGCGG AGAGGCGTCC 
    TGGAAGGCCT ACCTGGAGGT GCAAGAGTTT GGTGCTCCAG TTCAACCGGG CCGTCCCACA 
    GATCCCAACC TCATCCCCAG CGCCCCATCT AAACCAGACG TCACGGATGT CAGCCGCACC 
    TCCGTGTCTC TTTCCTGGAA GCCGAATCTG AACGCTGGAG CCACACCCAC CTCATACGTC 
    ATTGAGGCCT TCAGCCATGC AGCAGGCAGC AGCTGGCAGA CGCTCGCCGA TCATGTGAAG 
    ACGGAAAGCT TCGTGCTTAA GGGTCTGAAG CCAAGTGCCG TCTACCTGTT TCTGGTGAGA 
    GCGGCCAATG CGTACGGCCT GAGCGACCCC AGTCCCATCA GCGACGCTGT CAAAACACAA 
    GACGTCCCAC CAACCAGCCA GGGAGTGGAG CATGGCCAGA TCCAGAGAGA ACTGGGAGAT 
    GTCGTCATTC ACCTTCACAA TCCCACAATT CTCTCATCGT CTTCCGTCAG AGTCCAGTGG 
    ACTGTGGAGC AGCAGTCTCA GTATATTCAG GGCTATAAGG TGATGTACCG TCCGTCTCCA 
    GAAGGCGCTC CGCAGCGAGT GGATTGGGCC ATGTTTGAGG TCGGAGCCCC GGGGGAGCAC 
    AGCGCTGTCG TCACCCAGCT CAAGAAGGGC ATCACCTATG AGTTCAAAGT GCGCCCTTTC 
    TTCAATGAGT TCCAGGGCAC CGACAGCGAC ATCAAAGTGG GAAAGACCCT GGAGGAGGCT 
    CCCAGCGCTG CTCCCAGAGG CGTCACCGTA ACAGGAAGTG GAGACAACGG CACAGCAGTC 
    CTGGTCGCCT GGCAACCGCC GCCAGAGGAG GAGCAGAACG GCGTTGTGCA GGAGTACAAG 
    ATCTGGTGTC TTGGTAATGA AAGCCGATAC CACATCAACC GCACAGTGGA TGGTTCTACA 
    CACTCGGTGT TGATTCCTGG TCTGGTGGCA GGAGTCACAT ACAGGCTAGA GGTGGCAGCA 
    GGCACTGGGG CCGGACCCGG GGTCAAGAGC GACCCTACGT CCTTCCAGCT TGACTCTTCT 
    GGCCGTGTTC TCCCGCCGCC GCTGGACGAC GATAACACCA TTTCCCAGCA GATCTCAGAC 
    GTGGTGCGTC AGCCGGCTTT CATCGCGGGC ATCGGCGCCG CCTGCTGGAT CATCCTGATG 
    GTGTTCAGCA TCTGGCTATA CCGCCACCGC AAGAAGAGGA ACGGCCTCAG CAGCAGCTAC 
    GCCGGCATCC GCAAAGTGCC CTCCTTCACC TTCACCCCCA CAGTGGCCTA TCAGAGAGGC 
    GGAGAGGCCG TCAGCAGTGC AGGAAGGCCG GGTTTGTTGA ATATAGGTGA ACCAGCTACA 
    CAGCCCTGGC TTGCGGACAC CTGGCCCAAC AGCTGCTCCA ATCACAATGA CTGCACTATT 
    AACTGCTGCA CCGGCAGCAA TGGAAACAGC GACAGCAACC TGGCAACTTA CAGCCGCCCA 
    GCCGACTGCA TCGCCAACTA CAGCAACCAG ATGGACAACA AGGCCACCAA CCTGCTGGCT 
    CCAGATACTG GGCTTTACAG CGACGTTGAC CTTTCAAACA AGATCAACGA GATGAAGACC 
    TTCAACAGCC CCAACCTGAA GGACGGGCGT TTTATGGGGC CTGGTGGTCA ACCCACACCC 
    TACGCCACCA CTCAGCTCAT TCAATCCAGC CTGGCCAACA ACAACATGAA CAGCAGCAGT 
    GGCGGAAGCG GAGGAGGAAG CGCTGACTTT AATGAGAAGC AGAGCTGGAA GAGCAGCCAG 
    GGAACACCTC AGAAACAGGC CGACATCAAC ACACCACTGC AGTATAACGT CATGGAACAA 
    AACAAACTCA ACAAGGACCA CTACCGAGGT GAGGGTCCCA TCTCAGTCAC CATCCCTTAT 
    AACCAGGCCC AGGACGTCCT CACAGGAGAA ACCTACAGCA GCTCGGACAG AGGGAGCAGC 
    ACCTCTGGAA GTCAAGGGCA GAAAAAGGGT GGACGAACAA CTAAACAAGC CAAACAGAAT 
    CCAATGAATT GGACTGAACT GTTGCCTCCT CCTCCGGTTA ATCCGCCGCC ATGTCAAGAA 
    TACACCCTGG TGATGGACGA CAGTTACGAC ACAGACCTGC AGTGCCCTTT GCCGCCGTCT 
    CGGATGTATC TGCAACCTGA CGAGTTGGAA GAGGAGGAGG ACGAGGTGGA GCTGGAGCGT 
    GGACCTACAC CTCCTGTACG GGGAGCTGCT TCTTCACCTG CTGCCGTGTC CTACAGCCAC 
    CAGTCCACCG CAACCCTAAC CCCGTCCCCT CAGGAGGAGA TGCATCCCAT GCTGCAGGAC 
    AACCCTGATC TACATGAGCG CAGGAGACTG TTAGTGAGTC CACCTCCTCC ACCACGACCT 
    CTCTCTCCTC CACACACATA CGGCTACATC TCCAGTCCCC TCGGCCTGGA CACGGACGGC 
    CTAGAGGAAG AGGATGATGA TGAAGAAGAG GGTGACGAAA CGGACGCAGA GGTAGCTCGA 
    GTCCACCAGC ACCACATGAT GCACCCTCAC CAGCAGCACT TGCCCCAGCA GAGGGCTCTC 
    CTGCGGGGTC TGGAACAAAC CCCGGCCTCC AGCACCTGTG ACCTGGAGAG CTCTGTGACT 
    GGGTCTATGA TTAACGGATG GGGTTCGGCT TCAGAAGAGG ACAACGCCTC GTCAGGCCGT 
    TCCAGTGCAG TCAGCTCATC GGATGGATCG TTCTTTACCG ATGCCGATTT CGCCCAGGCT 
    GTGGCAGCTG CGGCTGAATA TGCTGGTCTT CGAGTGGCAA AGCATCCTGG GAAAGGACAT 
    CATCAGCTTC CAGTGTCTGG AGCACGGAAA TACCAAAACC TCCCTGCGGG ACACCGTCCA 
    GGCAGCCCAG TGTCCACAGA CAGTAACGTT AGCGCAGCCC CGGGTCAGAG GAGGCCCCTG 
    AAGAGGCAAA AACAACATCC AGCTGCTCAG ACAGGCTACC AGCGCCGAGA GGCCTACACA 
    GAGGATCTCC CCCCTCCTCC GATTCCTCCT CCAGCCATGC TGAAGTCTCC AACACACCCG 
    GCCAAAACCA TCCCCGAGGG TCCACGGGGG GCCATGTCGC CAAGATCCAG CTCTGCGGAT 
    GGCAAGGACA GGCGAACGGG TGCTGGCGGG CCCAGGGTCA GGGACGGTTC AGACACTCGG 
    ACCAGCTCTT CAGAGCGGAG GGATGGCCAA GATGTACAGA GGGGTCAGAG AACGGGCAAG 
    AATAGCAAAC AAGATGAAGC CGTGAAGAGC CGCCAGCACC CAGGCTCAGA GGACATCCTA 
    CCTTACAGTC GTCCTTCTTT CCCAGCCGTC CACAGTCCTC GAGGAGAACG GGACCCCAGT 
    TCCTCCAGTT CACTGTCCTC TCGGGGGTCT GGCGGGCGAC GGAGAGGTGA AGGGCCACCA 
    GGGGCCCGCA GAAACCCTGC AGACATTGGG CTAAACACCA TAGGAGCCTT CCAGACTGGA 
    GGGGACGAGC TTGAGATGGT GGAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214699.1
    CDS766..5715
    Translation

    Target ORF information:

    RefSeq Version XM_017359210.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359210.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTGTGTCTG TTCCTCCAGT GTTTGTGGTC 
    CGACCCAGGA ATCAGGTGGC GGGTATGGGC CGCACCGTCA CCTTTCAGTG TGAAGCCACA 
    GGAAGTCCTC AGCCTGCAAT ATTCTGGCAG AAAGAAGGCA GTCAGAATCT CCTGTTCTCG 
    TCTCAGCCTC CTCCTCCGTC CAGCAGATTC TCTGTGTCTC AGACTGGAGA TCTGACCATC 
    ACAGCAGTTG AGCGTTCAGA TGGAGGATAT TATAGCTGTC AGGCTCTCAA CATCGCTGGC 
    AGTGTGATCA CCAAAGCCCT GCTGGAGGTT ACAGACGTGA TTTCAGACAG GCCTCCACCA 
    ATCGTCCGTC AGGGTCCAAC CAACCAGACT GTGGCAGTGG ACGGGACGGT GGTTCTGAGC 
    TGCCAAGCCA CCGGGACACC GATTCCTACC ATCTTATGGA GGAAAGATGG AGTTCTGGTG 
    TCCACACATG ATTCCCGACT CAAGCAGCTG GACACTGGAG CCCTGCAGAT TCGATACGCA 
    AAGCTGGGCG ACACTGGCAT GTACACCTGC ACGGCTTCCA CCCCGAGCGG AGAGGCGTCC 
    TGGAAGGCCT ACCTGGAGGT GCAAGAGTTT GGTGCTCCAG TTCAACCGGG CCGTCCCACA 
    GATCCCAACC TCATCCCCAG CGCCCCATCT AAACCAGACG TCACGGATGT CAGCCGCACC 
    TCCGTGTCTC TTTCCTGGAA GCCGAATCTG AACGCTGGAG CCACACCCAC CTCATACGTC 
    ATTGAGGCCT TCAGCCATGC AGCAGGCAGC AGCTGGCAGA CGCTCGCCGA TCATGTGAAG 
    ACGGAAAGCT TCGTGCTTAA GGGTCTGAAG CCAAGTGCCG TCTACCTGTT TCTGGTGAGA 
    GCGGCCAATG CGTACGGCCT GAGCGACCCC AGTCCCATCA GCGACGCTGT CAAAACACAA 
    GACGTCCCAC CAACCAGCCA GGGAGTGGAG CATGGCCAGA TCCAGAGAGA ACTGGGAGAT 
    GTCGTCATTC ACCTTCACAA TCCCACAATT CTCTCATCGT CTTCCGTCAG AGTCCAGTGG 
    ACTGTGGAGC AGCAGTCTCA GTATATTCAG GGCTATAAGG TGATGTACCG TCCGTCTCCA 
    GAAGGCGCTC CGCAGCGAGT GGATTGGGCC ATGTTTGAGG TCGGAGCCCC GGGGGAGCAC 
    AGCGCTGTCG TCACCCAGCT CAAGAAGGGC ATCACCTATG AGTTCAAAGT GCGCCCTTTC 
    TTCAATGAGT TCCAGGGCAC CGACAGCGAC ATCAAAGTGG GAAAGACCCT GGAGGAGGCT 
    CCCAGCGCTG CTCCCAGAGG CGTCACCGTA ACAGGAAGTG GAGACAACGG CACAGCAGTC 
    CTGGTCGCCT GGCAACCGCC GCCAGAGGAG GAGCAGAACG GCGTTGTGCA GGAGTACAAG 
    ATCTGGTGTC TTGGTAATGA AAGCCGATAC CACATCAACC GCACAGTGGA TGGTTCTACA 
    CACTCGGTGT TGATTCCTGG TCTGGTGGCA GGAGTCACAT ACAGGCTAGA GGTGGCAGCA 
    GGCACTGGGG CCGGACCCGG GGTCAAGAGC GACCCTACGT CCTTCCAGCT TGACTCTTCT 
    GGCCGTGTTC TCCCGCCGCC GCTGGACGAC GATAACACCA TTTCCCAGCA GATCTCAGAC 
    GTGGTGCGTC AGCCGGCTTT CATCGCGGGC ATCGGCGCCG CCTGCTGGAT CATCCTGATG 
    GTGTTCAGCA TCTGGCTATA CCGCCACCGC AAGAAGAGGA ACGGCCTCAG CAGCAGCTAC 
    GCCGGCATCC GCAAAGTGCC CTCCTTCACC TTCACCCCCA CAGTGGCCTA TCAGAGAGGC 
    GGAGAGGCCG TCAGCAGTGC AGGAAGGCCG GGTTTGTTGA ATATAGGTGA ACCAGCTACA 
    CAGCCCTGGC TTGCGGACAC CTGGCCCAAC AGCTGCTCCA ATCACAATGA CTGCACTATT 
    AACTGCTGCA CCGGCAGCAA TGGAAACAGC GACAGCAACC TGGCAACTTA CAGCCGCCCA 
    GCCGACTGCA TCGCCAACTA CAGCAACCAG ATGGACAACA AGGCCACCAA CCTGCTGGCT 
    CCAGATACTG GGCTTTACAG CGACGTTGAC CTTTCAAACA AGATCAACGA GATGAAGACC 
    TTCAACAGCC CCAACCTGAA GGACGGGCGT TTTATGGGGC CTGGTGGTCA ACCCACACCC 
    TACGCCACCA CTCAGCTCAT TCAATCCAGC CTGGCCAACA ACAACATGAA CAGCAGCAGT 
    GGCGGAAGCG GAGGAGGAAG CGCTGACTTT AATGAGAAGC AGAGCTGGAA GAGCAGCCAG 
    GGAACACCTC AGAAACAGGC CGACATCAAC ACACCACTGC AGTATAACGT CATGGAACAA 
    AACAAACTCA ACAAGGACCA CTACCGAGGT GAGGGTCCCA TCTCAGTCAC CATCCCTTAT 
    AACCAGGCCC AGGACGTCCT CACAGGAGAA ACCTACAGCA GCTCGGACAG AGGGAGCAGC 
    ACCTCTGGAA GTCAAGGGCA GAAAAAGGGT GGACGAACAA CTAAACAAGC CAAACAGAAT 
    CCAATGAATT GGACTGAACT GTTGCCTCCT CCTCCGGTTA ATCCGCCGCC ATGTCAAGAA 
    TACACCCTGG TGATGGACGA CAGTTACGAC ACAGACCTGC AGTGCCCTTT GCCGCCGTCT 
    CGGATGTATC TGCAACCTGA CGAGTTGGAA GAGGAGGAGG ACGAGGTGGA GCTGGAGCGT 
    GGACCTACAC CTCCTGTACG GGGAGCTGCT TCTTCACCTG CTGCCGTGTC CTACAGCCAC 
    CAGTCCACCG CAACCCTAAC CCCGTCCCCT CAGGAGGAGA TGCATCCCAT GCTGCAGGAC 
    AACCCTGATC TACATGAGCG CAGGAGACTG TTAGTGAGTC CACCTCCTCC ACCACGACCT 
    CTCTCTCCTC CACACACATA CGGCTACATC TCCAGTCCCC TCGGCCTGGA CACGGACGGC 
    CTAGAGGAAG AGGATGATGA TGAAGAAGAG GGTGACGAAA CGGACGCAGA GGTAGCTCGA 
    GTCCACCAGC ACCACATGAT GCACCCTCAC CAGCAGCACT TGCCCCAGCA GAGGGCTCTC 
    CTGCGGGGTC TGGAACAAAC CCCGGCCTCC AGCACCTGTG ACCTGGAGAG CTCTGTGACT 
    GGGTCTATGA TTAACGGATG GGGTTCGGCT TCAGAAGAGG ACAACGCCTC GTCAGGCCGT 
    TCCAGTGCAG TCAGCTCATC GGATGGATCG TTCTTTACCG ATGCCGATTT CGCCCAGGCT 
    GTGGCAGCTG CGGCTGAATA TGCTGGTCTT CGAGTGGCAA AGCATCCTGG GAAAGGACAT 
    CATCAGCTTC CAGTGTCTGG AGCACGGAAA TACCAAAACC TCCCTGCGGG ACACCGTCCA 
    GGCAGCCCAG TGTCCACAGA CAGTAACGTT AGCGCAGCCC CGGGTCAGAG GAGGCCCCTG 
    AAGAGGCAAA AACAACATCC AGCTGCTCAG ACAGGCTACC AGCGCCGAGA GGCCTACACA 
    GAGGATCTCC CCCCTCCTCC GATTCCTCCT CCAGCCATGC TGAAGTCTCC AACACACCCG 
    GCCAAAACCA TCCCCGAGGG TCCACGGGGG GCCATGTCGC CAAGATCCAG CTCTGCGGAT 
    GGCAAGGACA GGCGAACGGG TGCTGGCGGG CCCAGGGTCA GGGACGGTTC AGACACTCGG 
    ACCAGCTCTT CAGAGCGGAG GGATGGCCAA GATGTACAGA GGGGTCAGAG AACGGGCAAG 
    AATAGCAAAC AAGATGAAGC CGTGAAGAGC CGCCAGCACC CAGGCTCAGA GGACATCCTA 
    CCTTACAGTC GTCCTTCTTT CCCAGCCGTC CACAGTCCTC GAGGAGAACG GGACCCCAGT 
    TCCTCCAGTT CACTGTCCTC TCGGGGGTCT GGCGGGCGAC GGAGAGGTGA AGGGCCACCA 
    GGGGCCCGCA GAAACCCTGC AGACATTGGG CTAAACACCA TAGGAGCCTT CCAGACTGGA 
    GGGGACGAGC TTGAGATGGT GGAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359211.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214700.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID ODa55062
    Clone ID Related Accession (Same CDS sequence) XM_017359211.1 , XM_017359211.2
    Accession Version XM_017359211.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTCCTCCAG TGTTTGTGGT CCGACCCAGG 
    AATCAGGTGG CGGGTATGGG CCGCACCGTC ACCTTTCAGT GTGAAGCCAC AGGAAGTCCT 
    CAGCCTGCAA TATTCTGGCA GAAAGAAGGC AGTCAGAATC TCCTGTTCTC GTCTCAGCCT 
    CCTCCTCCGT CCAGCAGATT CTCTGTGTCT CAGACTGGAG ATCTGACCAT CACAGCAGTT 
    GAGCGTTCAG ATGGAGGATA TTATAGCTGT CAGGCTCTCA ACATCGCTGG CAGTGTGATC 
    ACCAAAGCCC TGCTGGAGGT TACAGACGTG ATTTCAGACA GGCCTCCACC AATCGTCCGT 
    CAGGGTCCAA CCAACCAGAC TGTGGCAGTG GACGGGACGG TGGTTCTGAG CTGCCAAGCC 
    ACCGGGACAC CGATTCCTAC CATCTTATGG AGGAAAGATG GAGTTCTGGT GTCCACACAT 
    GATTCCCGAC TCAAGCAGCT GGACACTGGA GCCCTGCAGA TTCGATACGC AAAGCTGGGC 
    GACACTGGCA TGTACACCTG CACGGCTTCC ACCCCGAGCG GAGAGGCGTC CTGGAAGGCC 
    TACCTGGAGG TGCAAGAGTT TGGTGCTCCA GTTCAACCGG GCCGTCCCAC AGATCCCAAC 
    CTCATCCCCA GCGCCCCATC TAAACCAGAC GTCACGGATG TCAGCCGCAC CTCCGTGTCT 
    CTTTCCTGGA AGCCGAATCT GAACGCTGGA GCCACACCCA CCTCATACGT CATTGAGGCC 
    TTCAGCCATG CAGCAGGCAG CAGCTGGCAG ACGCTCGCCG ATCATGTGAA GACGGAAAGC 
    TTCGTGCTTA AGGGTCTGAA GCCAAGTGCC GTCTACCTGT TTCTGGTGAG AGCGGCCAAT 
    GCGTACGGCC TGAGCGACCC CAGTCCCATC AGCGACGCTG TCAAAACACA AGACGTCCCA 
    CCAACCAGCC AGGGAGTGGA GCATGGCCAG ATCCAGAGAG AACTGGGAGA TGTCGTCATT 
    CACCTTCACA ATCCCACAAT TCTCTCATCG TCTTCCGTCA GAGTCCAGTG GACTGTGGAG 
    CAGCAGTCTC AGTATATTCA GGGCTATAAG GTGATGTACC GTCCGTCTCC AGAAGGCGCT 
    CCGCAGCGAG TGGATTGGGC CATGTTTGAG GTCGGAGCCC CGGGGGAGCA CAGCGCTGTC 
    GTCACCCAGC TCAAGAAGGG CATCACCTAT GAGTTCAAAG TGCGCCCTTT CTTCAATGAG 
    TTCCAGGGCA CCGACAGCGA CATCAAAGTG GGAAAGACCC TGGAGGAGGC TCCCAGCGCT 
    GCTCCCAGAG GCGTCACCGT AACAGGAAGT GGAGACAACG GCACAGCAGT CCTGGTCGCC 
    TGGCAACCGC CGCCAGAGGA GGAGCAGAAC GGCGTTGTGC AGGAGTACAA GATCTGGTGT 
    CTTGGTAATG AAAGCCGATA CCACATCAAC CGCACAGTGG ATGGTTCTAC ACACTCGGTG 
    TTGATTCCTG GTCTGGTGGC AGGAGTCACA TACAGGCTAG AGGTGGCAGC AGGCACTGGG 
    GCCGGACCCG GGGTCAAGAG CGACCCTACG TCCTTCCAGC TTGACTCTTC TGGCCGTGTT 
    CTCCCGCCGC CGCTGGACGA CGATAACACC ATTTCCCAGC AGATCTCAGA CGTGGTGCGT 
    CAGCCGGCTT TCATCGCGGG CATCGGCGCC GCCTGCTGGA TCATCCTGAT GGTGTTCAGC 
    ATCTGGCTAT ACCGCCACCG CAAGAAGAGG AACGGCCTCA GCAGCAGCTA CGCCGGCATC 
    CGCAAAGTGC CCTCCTTCAC CTTCACCCCC ACAGTGGCCT ATCAGAGAGG CGGAGAGGCC 
    GTCAGCAGTG CAGGAAGGCC GGGTTTGTTG AATATAGGTG AACCAGCTAC ACAGCCCTGG 
    CTTGCGGACA CCTGGCCCAA CAGCTGCTCC AATCACAATG ACTGCACTAT TAACTGCTGC 
    ACCGGCAGCA ATGGAAACAG CGACAGCAAC CTGGCAACTT ACAGCCGCCC AGCCGACTGC 
    ATCGCCAACT ACAGCAACCA GATGGACAAC AAGGCCACCA ACCTGCTGGC TCCAGATACT 
    GGGCTTTACA GCGACGTTGA CCTTTCAAAC AAGATCAACG AGATGAAGAC CTTCAACAGC 
    CCCAACCTGA AGGACGGGCG TTTTATGGGG CCTGGTGGTC AACCCACACC CTACGCCACC 
    ACTCAGCTCA TTCAATCCAG CCTGGCCAAC AACAACATGA ACAGCAGCAG TGGCGGAAGC 
    GGAGGAGGAA GCGCTGACTT TAATGAGAAG CAGAGCTGGA AGAGCAGCCA GGGAACACCT 
    CAGAAACAGG CCGACATCAA CACACCACTG CAGTATAACG TCATGGAACA AAACAAACTC 
    AACAAGGACC ACTACCGAGG TGAGGGTCCC ATCTCAGTCA CCATCCCTTA TAACCAGGCC 
    CAGGACGTCC TCACAGGAGA AACCTACAGC AGCTCGGACA GAGGGAGCAG CACCTCTGGA 
    AGTCAAGGGC AGAAAAAGGG TGGACGAACA ACTAAACAAG CCAAACAGAA TCCAATGAAT 
    TGGACTGAAC TGTTGCCTCC TCCTCCGGTT AATCCGCCGC CATGTCAAGA ATACACCCTG 
    GTGATGGACG ACAGTTACGA CACAGACCTG CAGTGCCCTT TGCCGCCGTC TCGGATGTAT 
    CTGCAACCTG ACGAGTTGGA AGAGGAGGAG GACGAGGTGG AGCTGGAGCG TGGACCTACA 
    CCTCCTGTAC GGGGAGCTGC TTCTTCACCT GCTGCCGTGT CCTACAGCCA CCAGTCCACC 
    GCAACCCTAA CCCCGTCCCC TCAGGAGGAG ATGCATCCCA TGCTGCAGGA CAACCCTGAT 
    CTACATGAGC GCAGGAGACT GTTAGTGAGT CCACCTCCTC CACCACGACC TCTCTCTCCT 
    CCACACACAT ACGGCTACAT CTCCAGTCCC CTCGGCCTGG ACACGGACGG CCTAGAGGAA 
    GAGGATGATG ATGAAGAAGA GGGTGACGAA ACGGACGCAG AGGTAGCTCG AGTCCACCAG 
    CACCACATGA TGCACCCTCA CCAGCAGCAC TTGCCCCAGC AGAGGGCTCT CCTGCGGGGT 
    CTGGAACAAA CCCCGGCCTC CAGCACCTGT GACCTGGAGA GCTCTGTGAC TGGGTCTATG 
    ATTAACGGAT GGGGTTCGGC TTCAGAAGAG GACAACGCCT CGTCAGGCCG TTCCAGTGCA 
    GTCAGCTCAT CGGATGGATC GTTCTTTACC GATGCCGATT TCGCCCAGGC TGTGGCAGCT 
    GCGGCTGAAT ATGCTGGTCT TCGAGTGGCA AAGCATCCTG GGAAAGGACA TCATCAGCTT 
    CCAGTGTCTG GAGCAGCACG GAAATACCAA AACCTCCCTG CGGGACACCG TCCAGGCAGC 
    CCAGTGTCCA CAGACAGTAA CGTTAGCGCA GCCCCGGGTC AGAGGAGGCC CCTGAAGAGG 
    CAAAAACAAC ATCCAGCTGC TCAGACAGGC TACCAGCGCC GAGAGGCCTA CACAGAGGAT 
    CTCCCCCCTC CTCCGATTCC TCCTCCAGCC ATGCTGAAGT CTCCAACACA CCCGGCCAAA 
    ACCATCCCCG AGGGTCCACG GGGGGCCATG TCGCCAAGAT CCAGCTCTGC GGATGGCAAG 
    GACAGGCGAA CGGGTGCTGG CGGGCCCAGG GTCAGGGACG GTTCAGACAC TCGGACCAGC 
    TCTTCAGAGC GGAGGGATGG CCAAGATGTA CAGAGGGGTC AGAGAACGGG CAAGAATAGC 
    AAACAAGATG AAGCCGTGAA GAGCCGCCAG CACCCAGGCT CAGAGGACAT CCTACCTTAC 
    AGTCGTCCTT CTTTCCCAGC CGTCCACAGT CCTCGAGGAG AACGGGACCC CAGTTCCTCC 
    AGTTCACTGT CCTCTCGGGG GTCTGGCGGG CGACGGAGAG GTGAAGGGCC ACCAGGGGCC 
    CGCAGAAACC CTGCAGACAT TGGGCTAAAC ACCATAGGAG CCTTCCAGAC TGGAGGGGAC 
    GAGCTTGAGA TGGTGGAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214700.1
    CDS942..5885
    Translation

    Target ORF information:

    RefSeq Version XM_017359211.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359211.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTCCTCCAG TGTTTGTGGT CCGACCCAGG 
    AATCAGGTGG CGGGTATGGG CCGCACCGTC ACCTTTCAGT GTGAAGCCAC AGGAAGTCCT 
    CAGCCTGCAA TATTCTGGCA GAAAGAAGGC AGTCAGAATC TCCTGTTCTC GTCTCAGCCT 
    CCTCCTCCGT CCAGCAGATT CTCTGTGTCT CAGACTGGAG ATCTGACCAT CACAGCAGTT 
    GAGCGTTCAG ATGGAGGATA TTATAGCTGT CAGGCTCTCA ACATCGCTGG CAGTGTGATC 
    ACCAAAGCCC TGCTGGAGGT TACAGACGTG ATTTCAGACA GGCCTCCACC AATCGTCCGT 
    CAGGGTCCAA CCAACCAGAC TGTGGCAGTG GACGGGACGG TGGTTCTGAG CTGCCAAGCC 
    ACCGGGACAC CGATTCCTAC CATCTTATGG AGGAAAGATG GAGTTCTGGT GTCCACACAT 
    GATTCCCGAC TCAAGCAGCT GGACACTGGA GCCCTGCAGA TTCGATACGC AAAGCTGGGC 
    GACACTGGCA TGTACACCTG CACGGCTTCC ACCCCGAGCG GAGAGGCGTC CTGGAAGGCC 
    TACCTGGAGG TGCAAGAGTT TGGTGCTCCA GTTCAACCGG GCCGTCCCAC AGATCCCAAC 
    CTCATCCCCA GCGCCCCATC TAAACCAGAC GTCACGGATG TCAGCCGCAC CTCCGTGTCT 
    CTTTCCTGGA AGCCGAATCT GAACGCTGGA GCCACACCCA CCTCATACGT CATTGAGGCC 
    TTCAGCCATG CAGCAGGCAG CAGCTGGCAG ACGCTCGCCG ATCATGTGAA GACGGAAAGC 
    TTCGTGCTTA AGGGTCTGAA GCCAAGTGCC GTCTACCTGT TTCTGGTGAG AGCGGCCAAT 
    GCGTACGGCC TGAGCGACCC CAGTCCCATC AGCGACGCTG TCAAAACACA AGACGTCCCA 
    CCAACCAGCC AGGGAGTGGA GCATGGCCAG ATCCAGAGAG AACTGGGAGA TGTCGTCATT 
    CACCTTCACA ATCCCACAAT TCTCTCATCG TCTTCCGTCA GAGTCCAGTG GACTGTGGAG 
    CAGCAGTCTC AGTATATTCA GGGCTATAAG GTGATGTACC GTCCGTCTCC AGAAGGCGCT 
    CCGCAGCGAG TGGATTGGGC CATGTTTGAG GTCGGAGCCC CGGGGGAGCA CAGCGCTGTC 
    GTCACCCAGC TCAAGAAGGG CATCACCTAT GAGTTCAAAG TGCGCCCTTT CTTCAATGAG 
    TTCCAGGGCA CCGACAGCGA CATCAAAGTG GGAAAGACCC TGGAGGAGGC TCCCAGCGCT 
    GCTCCCAGAG GCGTCACCGT AACAGGAAGT GGAGACAACG GCACAGCAGT CCTGGTCGCC 
    TGGCAACCGC CGCCAGAGGA GGAGCAGAAC GGCGTTGTGC AGGAGTACAA GATCTGGTGT 
    CTTGGTAATG AAAGCCGATA CCACATCAAC CGCACAGTGG ATGGTTCTAC ACACTCGGTG 
    TTGATTCCTG GTCTGGTGGC AGGAGTCACA TACAGGCTAG AGGTGGCAGC AGGCACTGGG 
    GCCGGACCCG GGGTCAAGAG CGACCCTACG TCCTTCCAGC TTGACTCTTC TGGCCGTGTT 
    CTCCCGCCGC CGCTGGACGA CGATAACACC ATTTCCCAGC AGATCTCAGA CGTGGTGCGT 
    CAGCCGGCTT TCATCGCGGG CATCGGCGCC GCCTGCTGGA TCATCCTGAT GGTGTTCAGC 
    ATCTGGCTAT ACCGCCACCG CAAGAAGAGG AACGGCCTCA GCAGCAGCTA CGCCGGCATC 
    CGCAAAGTGC CCTCCTTCAC CTTCACCCCC ACAGTGGCCT ATCAGAGAGG CGGAGAGGCC 
    GTCAGCAGTG CAGGAAGGCC GGGTTTGTTG AATATAGGTG AACCAGCTAC ACAGCCCTGG 
    CTTGCGGACA CCTGGCCCAA CAGCTGCTCC AATCACAATG ACTGCACTAT TAACTGCTGC 
    ACCGGCAGCA ATGGAAACAG CGACAGCAAC CTGGCAACTT ACAGCCGCCC AGCCGACTGC 
    ATCGCCAACT ACAGCAACCA GATGGACAAC AAGGCCACCA ACCTGCTGGC TCCAGATACT 
    GGGCTTTACA GCGACGTTGA CCTTTCAAAC AAGATCAACG AGATGAAGAC CTTCAACAGC 
    CCCAACCTGA AGGACGGGCG TTTTATGGGG CCTGGTGGTC AACCCACACC CTACGCCACC 
    ACTCAGCTCA TTCAATCCAG CCTGGCCAAC AACAACATGA ACAGCAGCAG TGGCGGAAGC 
    GGAGGAGGAA GCGCTGACTT TAATGAGAAG CAGAGCTGGA AGAGCAGCCA GGGAACACCT 
    CAGAAACAGG CCGACATCAA CACACCACTG CAGTATAACG TCATGGAACA AAACAAACTC 
    AACAAGGACC ACTACCGAGG TGAGGGTCCC ATCTCAGTCA CCATCCCTTA TAACCAGGCC 
    CAGGACGTCC TCACAGGAGA AACCTACAGC AGCTCGGACA GAGGGAGCAG CACCTCTGGA 
    AGTCAAGGGC AGAAAAAGGG TGGACGAACA ACTAAACAAG CCAAACAGAA TCCAATGAAT 
    TGGACTGAAC TGTTGCCTCC TCCTCCGGTT AATCCGCCGC CATGTCAAGA ATACACCCTG 
    GTGATGGACG ACAGTTACGA CACAGACCTG CAGTGCCCTT TGCCGCCGTC TCGGATGTAT 
    CTGCAACCTG ACGAGTTGGA AGAGGAGGAG GACGAGGTGG AGCTGGAGCG TGGACCTACA 
    CCTCCTGTAC GGGGAGCTGC TTCTTCACCT GCTGCCGTGT CCTACAGCCA CCAGTCCACC 
    GCAACCCTAA CCCCGTCCCC TCAGGAGGAG ATGCATCCCA TGCTGCAGGA CAACCCTGAT 
    CTACATGAGC GCAGGAGACT GTTAGTGAGT CCACCTCCTC CACCACGACC TCTCTCTCCT 
    CCACACACAT ACGGCTACAT CTCCAGTCCC CTCGGCCTGG ACACGGACGG CCTAGAGGAA 
    GAGGATGATG ATGAAGAAGA GGGTGACGAA ACGGACGCAG AGGTAGCTCG AGTCCACCAG 
    CACCACATGA TGCACCCTCA CCAGCAGCAC TTGCCCCAGC AGAGGGCTCT CCTGCGGGGT 
    CTGGAACAAA CCCCGGCCTC CAGCACCTGT GACCTGGAGA GCTCTGTGAC TGGGTCTATG 
    ATTAACGGAT GGGGTTCGGC TTCAGAAGAG GACAACGCCT CGTCAGGCCG TTCCAGTGCA 
    GTCAGCTCAT CGGATGGATC GTTCTTTACC GATGCCGATT TCGCCCAGGC TGTGGCAGCT 
    GCGGCTGAAT ATGCTGGTCT TCGAGTGGCA AAGCATCCTG GGAAAGGACA TCATCAGCTT 
    CCAGTGTCTG GAGCAGCACG GAAATACCAA AACCTCCCTG CGGGACACCG TCCAGGCAGC 
    CCAGTGTCCA CAGACAGTAA CGTTAGCGCA GCCCCGGGTC AGAGGAGGCC CCTGAAGAGG 
    CAAAAACAAC ATCCAGCTGC TCAGACAGGC TACCAGCGCC GAGAGGCCTA CACAGAGGAT 
    CTCCCCCCTC CTCCGATTCC TCCTCCAGCC ATGCTGAAGT CTCCAACACA CCCGGCCAAA 
    ACCATCCCCG AGGGTCCACG GGGGGCCATG TCGCCAAGAT CCAGCTCTGC GGATGGCAAG 
    GACAGGCGAA CGGGTGCTGG CGGGCCCAGG GTCAGGGACG GTTCAGACAC TCGGACCAGC 
    TCTTCAGAGC GGAGGGATGG CCAAGATGTA CAGAGGGGTC AGAGAACGGG CAAGAATAGC 
    AAACAAGATG AAGCCGTGAA GAGCCGCCAG CACCCAGGCT CAGAGGACAT CCTACCTTAC 
    AGTCGTCCTT CTTTCCCAGC CGTCCACAGT CCTCGAGGAG AACGGGACCC CAGTTCCTCC 
    AGTTCACTGT CCTCTCGGGG GTCTGGCGGG CGACGGAGAG GTGAAGGGCC ACCAGGGGCC 
    CGCAGAAACC CTGCAGACAT TGGGCTAAAC ACCATAGGAG CCTTCCAGAC TGGAGGGGAC 
    GAGCTTGAGA TGGTGGAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359211.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214700.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID ODa55062
    Clone ID Related Accession (Same CDS sequence) XM_017359211.1 , XM_017359211.2
    Accession Version XM_017359211.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359211.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTCCTCCAG TGTTTGTGGT CCGACCCAGG 
    AATCAGGTGG CGGGTATGGG CCGCACCGTC ACCTTTCAGT GTGAAGCCAC AGGAAGTCCT 
    CAGCCTGCAA TATTCTGGCA GAAAGAAGGC AGTCAGAATC TCCTGTTCTC GTCTCAGCCT 
    CCTCCTCCGT CCAGCAGATT CTCTGTGTCT CAGACTGGAG ATCTGACCAT CACAGCAGTT 
    GAGCGTTCAG ATGGAGGATA TTATAGCTGT CAGGCTCTCA ACATCGCTGG CAGTGTGATC 
    ACCAAAGCCC TGCTGGAGGT TACAGACGTG ATTTCAGACA GGCCTCCACC AATCGTCCGT 
    CAGGGTCCAA CCAACCAGAC TGTGGCAGTG GACGGGACGG TGGTTCTGAG CTGCCAAGCC 
    ACCGGGACAC CGATTCCTAC CATCTTATGG AGGAAAGATG GAGTTCTGGT GTCCACACAT 
    GATTCCCGAC TCAAGCAGCT GGACACTGGA GCCCTGCAGA TTCGATACGC AAAGCTGGGC 
    GACACTGGCA TGTACACCTG CACGGCTTCC ACCCCGAGCG GAGAGGCGTC CTGGAAGGCC 
    TACCTGGAGG TGCAAGAGTT TGGTGCTCCA GTTCAACCGG GCCGTCCCAC AGATCCCAAC 
    CTCATCCCCA GCGCCCCATC TAAACCAGAC GTCACGGATG TCAGCCGCAC CTCCGTGTCT 
    CTTTCCTGGA AGCCGAATCT GAACGCTGGA GCCACACCCA CCTCATACGT CATTGAGGCC 
    TTCAGCCATG CAGCAGGCAG CAGCTGGCAG ACGCTCGCCG ATCATGTGAA GACGGAAAGC 
    TTCGTGCTTA AGGGTCTGAA GCCAAGTGCC GTCTACCTGT TTCTGGTGAG AGCGGCCAAT 
    GCGTACGGCC TGAGCGACCC CAGTCCCATC AGCGACGCTG TCAAAACACA AGACGTCCCA 
    CCAACCAGCC AGGGAGTGGA GCATGGCCAG ATCCAGAGAG AACTGGGAGA TGTCGTCATT 
    CACCTTCACA ATCCCACAAT TCTCTCATCG TCTTCCGTCA GAGTCCAGTG GACTGTGGAG 
    CAGCAGTCTC AGTATATTCA GGGCTATAAG GTGATGTACC GTCCGTCTCC AGAAGGCGCT 
    CCGCAGCGAG TGGATTGGGC CATGTTTGAG GTCGGAGCCC CGGGGGAGCA CAGCGCTGTC 
    GTCACCCAGC TCAAGAAGGG CATCACCTAT GAGTTCAAAG TGCGCCCTTT CTTCAATGAG 
    TTCCAGGGCA CCGACAGCGA CATCAAAGTG GGAAAGACCC TGGAGGAGGC TCCCAGCGCT 
    GCTCCCAGAG GCGTCACCGT AACAGGAAGT GGAGACAACG GCACAGCAGT CCTGGTCGCC 
    TGGCAACCGC CGCCAGAGGA GGAGCAGAAC GGCGTTGTGC AGGAGTACAA GATCTGGTGT 
    CTTGGTAATG AAAGCCGATA CCACATCAAC CGCACAGTGG ATGGTTCTAC ACACTCGGTG 
    TTGATTCCTG GTCTGGTGGC AGGAGTCACA TACAGGCTAG AGGTGGCAGC AGGCACTGGG 
    GCCGGACCCG GGGTCAAGAG CGACCCTACG TCCTTCCAGC TTGACTCTTC TGGCCGTGTT 
    CTCCCGCCGC CGCTGGACGA CGATAACACC ATTTCCCAGC AGATCTCAGA CGTGGTGCGT 
    CAGCCGGCTT TCATCGCGGG CATCGGCGCC GCCTGCTGGA TCATCCTGAT GGTGTTCAGC 
    ATCTGGCTAT ACCGCCACCG CAAGAAGAGG AACGGCCTCA GCAGCAGCTA CGCCGGCATC 
    CGCAAAGTGC CCTCCTTCAC CTTCACCCCC ACAGTGGCCT ATCAGAGAGG CGGAGAGGCC 
    GTCAGCAGTG CAGGAAGGCC GGGTTTGTTG AATATAGGTG AACCAGCTAC ACAGCCCTGG 
    CTTGCGGACA CCTGGCCCAA CAGCTGCTCC AATCACAATG ACTGCACTAT TAACTGCTGC 
    ACCGGCAGCA ATGGAAACAG CGACAGCAAC CTGGCAACTT ACAGCCGCCC AGCCGACTGC 
    ATCGCCAACT ACAGCAACCA GATGGACAAC AAGGCCACCA ACCTGCTGGC TCCAGATACT 
    GGGCTTTACA GCGACGTTGA CCTTTCAAAC AAGATCAACG AGATGAAGAC CTTCAACAGC 
    CCCAACCTGA AGGACGGGCG TTTTATGGGG CCTGGTGGTC AACCCACACC CTACGCCACC 
    ACTCAGCTCA TTCAATCCAG CCTGGCCAAC AACAACATGA ACAGCAGCAG TGGCGGAAGC 
    GGAGGAGGAA GCGCTGACTT TAATGAGAAG CAGAGCTGGA AGAGCAGCCA GGGAACACCT 
    CAGAAACAGG CCGACATCAA CACACCACTG CAGTATAACG TCATGGAACA AAACAAACTC 
    AACAAGGACC ACTACCGAGG TGAGGGTCCC ATCTCAGTCA CCATCCCTTA TAACCAGGCC 
    CAGGACGTCC TCACAGGAGA AACCTACAGC AGCTCGGACA GAGGGAGCAG CACCTCTGGA 
    AGTCAAGGGC AGAAAAAGGG TGGACGAACA ACTAAACAAG CCAAACAGAA TCCAATGAAT 
    TGGACTGAAC TGTTGCCTCC TCCTCCGGTT AATCCGCCGC CATGTCAAGA ATACACCCTG 
    GTGATGGACG ACAGTTACGA CACAGACCTG CAGTGCCCTT TGCCGCCGTC TCGGATGTAT 
    CTGCAACCTG ACGAGTTGGA AGAGGAGGAG GACGAGGTGG AGCTGGAGCG TGGACCTACA 
    CCTCCTGTAC GGGGAGCTGC TTCTTCACCT GCTGCCGTGT CCTACAGCCA CCAGTCCACC 
    GCAACCCTAA CCCCGTCCCC TCAGGAGGAG ATGCATCCCA TGCTGCAGGA CAACCCTGAT 
    CTACATGAGC GCAGGAGACT GTTAGTGAGT CCACCTCCTC CACCACGACC TCTCTCTCCT 
    CCACACACAT ACGGCTACAT CTCCAGTCCC CTCGGCCTGG ACACGGACGG CCTAGAGGAA 
    GAGGATGATG ATGAAGAAGA GGGTGACGAA ACGGACGCAG AGGTAGCTCG AGTCCACCAG 
    CACCACATGA TGCACCCTCA CCAGCAGCAC TTGCCCCAGC AGAGGGCTCT CCTGCGGGGT 
    CTGGAACAAA CCCCGGCCTC CAGCACCTGT GACCTGGAGA GCTCTGTGAC TGGGTCTATG 
    ATTAACGGAT GGGGTTCGGC TTCAGAAGAG GACAACGCCT CGTCAGGCCG TTCCAGTGCA 
    GTCAGCTCAT CGGATGGATC GTTCTTTACC GATGCCGATT TCGCCCAGGC TGTGGCAGCT 
    GCGGCTGAAT ATGCTGGTCT TCGAGTGGCA AAGCATCCTG GGAAAGGACA TCATCAGCTT 
    CCAGTGTCTG GAGCAGCACG GAAATACCAA AACCTCCCTG CGGGACACCG TCCAGGCAGC 
    CCAGTGTCCA CAGACAGTAA CGTTAGCGCA GCCCCGGGTC AGAGGAGGCC CCTGAAGAGG 
    CAAAAACAAC ATCCAGCTGC TCAGACAGGC TACCAGCGCC GAGAGGCCTA CACAGAGGAT 
    CTCCCCCCTC CTCCGATTCC TCCTCCAGCC ATGCTGAAGT CTCCAACACA CCCGGCCAAA 
    ACCATCCCCG AGGGTCCACG GGGGGCCATG TCGCCAAGAT CCAGCTCTGC GGATGGCAAG 
    GACAGGCGAA CGGGTGCTGG CGGGCCCAGG GTCAGGGACG GTTCAGACAC TCGGACCAGC 
    TCTTCAGAGC GGAGGGATGG CCAAGATGTA CAGAGGGGTC AGAGAACGGG CAAGAATAGC 
    AAACAAGATG AAGCCGTGAA GAGCCGCCAG CACCCAGGCT CAGAGGACAT CCTACCTTAC 
    AGTCGTCCTT CTTTCCCAGC CGTCCACAGT CCTCGAGGAG AACGGGACCC CAGTTCCTCC 
    AGTTCACTGT CCTCTCGGGG GTCTGGCGGG CGACGGAGAG GTGAAGGGCC ACCAGGGGCC 
    CGCAGAAACC CTGCAGACAT TGGGCTAAAC ACCATAGGAG CCTTCCAGAC TGGAGGGGAC 
    GAGCTTGAGA TGGTGGAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214700.1
    CDS767..5710
    Translation

    Target ORF information:

    RefSeq Version XM_017359211.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359211.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTCCTCCAG TGTTTGTGGT CCGACCCAGG 
    AATCAGGTGG CGGGTATGGG CCGCACCGTC ACCTTTCAGT GTGAAGCCAC AGGAAGTCCT 
    CAGCCTGCAA TATTCTGGCA GAAAGAAGGC AGTCAGAATC TCCTGTTCTC GTCTCAGCCT 
    CCTCCTCCGT CCAGCAGATT CTCTGTGTCT CAGACTGGAG ATCTGACCAT CACAGCAGTT 
    GAGCGTTCAG ATGGAGGATA TTATAGCTGT CAGGCTCTCA ACATCGCTGG CAGTGTGATC 
    ACCAAAGCCC TGCTGGAGGT TACAGACGTG ATTTCAGACA GGCCTCCACC AATCGTCCGT 
    CAGGGTCCAA CCAACCAGAC TGTGGCAGTG GACGGGACGG TGGTTCTGAG CTGCCAAGCC 
    ACCGGGACAC CGATTCCTAC CATCTTATGG AGGAAAGATG GAGTTCTGGT GTCCACACAT 
    GATTCCCGAC TCAAGCAGCT GGACACTGGA GCCCTGCAGA TTCGATACGC AAAGCTGGGC 
    GACACTGGCA TGTACACCTG CACGGCTTCC ACCCCGAGCG GAGAGGCGTC CTGGAAGGCC 
    TACCTGGAGG TGCAAGAGTT TGGTGCTCCA GTTCAACCGG GCCGTCCCAC AGATCCCAAC 
    CTCATCCCCA GCGCCCCATC TAAACCAGAC GTCACGGATG TCAGCCGCAC CTCCGTGTCT 
    CTTTCCTGGA AGCCGAATCT GAACGCTGGA GCCACACCCA CCTCATACGT CATTGAGGCC 
    TTCAGCCATG CAGCAGGCAG CAGCTGGCAG ACGCTCGCCG ATCATGTGAA GACGGAAAGC 
    TTCGTGCTTA AGGGTCTGAA GCCAAGTGCC GTCTACCTGT TTCTGGTGAG AGCGGCCAAT 
    GCGTACGGCC TGAGCGACCC CAGTCCCATC AGCGACGCTG TCAAAACACA AGACGTCCCA 
    CCAACCAGCC AGGGAGTGGA GCATGGCCAG ATCCAGAGAG AACTGGGAGA TGTCGTCATT 
    CACCTTCACA ATCCCACAAT TCTCTCATCG TCTTCCGTCA GAGTCCAGTG GACTGTGGAG 
    CAGCAGTCTC AGTATATTCA GGGCTATAAG GTGATGTACC GTCCGTCTCC AGAAGGCGCT 
    CCGCAGCGAG TGGATTGGGC CATGTTTGAG GTCGGAGCCC CGGGGGAGCA CAGCGCTGTC 
    GTCACCCAGC TCAAGAAGGG CATCACCTAT GAGTTCAAAG TGCGCCCTTT CTTCAATGAG 
    TTCCAGGGCA CCGACAGCGA CATCAAAGTG GGAAAGACCC TGGAGGAGGC TCCCAGCGCT 
    GCTCCCAGAG GCGTCACCGT AACAGGAAGT GGAGACAACG GCACAGCAGT CCTGGTCGCC 
    TGGCAACCGC CGCCAGAGGA GGAGCAGAAC GGCGTTGTGC AGGAGTACAA GATCTGGTGT 
    CTTGGTAATG AAAGCCGATA CCACATCAAC CGCACAGTGG ATGGTTCTAC ACACTCGGTG 
    TTGATTCCTG GTCTGGTGGC AGGAGTCACA TACAGGCTAG AGGTGGCAGC AGGCACTGGG 
    GCCGGACCCG GGGTCAAGAG CGACCCTACG TCCTTCCAGC TTGACTCTTC TGGCCGTGTT 
    CTCCCGCCGC CGCTGGACGA CGATAACACC ATTTCCCAGC AGATCTCAGA CGTGGTGCGT 
    CAGCCGGCTT TCATCGCGGG CATCGGCGCC GCCTGCTGGA TCATCCTGAT GGTGTTCAGC 
    ATCTGGCTAT ACCGCCACCG CAAGAAGAGG AACGGCCTCA GCAGCAGCTA CGCCGGCATC 
    CGCAAAGTGC CCTCCTTCAC CTTCACCCCC ACAGTGGCCT ATCAGAGAGG CGGAGAGGCC 
    GTCAGCAGTG CAGGAAGGCC GGGTTTGTTG AATATAGGTG AACCAGCTAC ACAGCCCTGG 
    CTTGCGGACA CCTGGCCCAA CAGCTGCTCC AATCACAATG ACTGCACTAT TAACTGCTGC 
    ACCGGCAGCA ATGGAAACAG CGACAGCAAC CTGGCAACTT ACAGCCGCCC AGCCGACTGC 
    ATCGCCAACT ACAGCAACCA GATGGACAAC AAGGCCACCA ACCTGCTGGC TCCAGATACT 
    GGGCTTTACA GCGACGTTGA CCTTTCAAAC AAGATCAACG AGATGAAGAC CTTCAACAGC 
    CCCAACCTGA AGGACGGGCG TTTTATGGGG CCTGGTGGTC AACCCACACC CTACGCCACC 
    ACTCAGCTCA TTCAATCCAG CCTGGCCAAC AACAACATGA ACAGCAGCAG TGGCGGAAGC 
    GGAGGAGGAA GCGCTGACTT TAATGAGAAG CAGAGCTGGA AGAGCAGCCA GGGAACACCT 
    CAGAAACAGG CCGACATCAA CACACCACTG CAGTATAACG TCATGGAACA AAACAAACTC 
    AACAAGGACC ACTACCGAGG TGAGGGTCCC ATCTCAGTCA CCATCCCTTA TAACCAGGCC 
    CAGGACGTCC TCACAGGAGA AACCTACAGC AGCTCGGACA GAGGGAGCAG CACCTCTGGA 
    AGTCAAGGGC AGAAAAAGGG TGGACGAACA ACTAAACAAG CCAAACAGAA TCCAATGAAT 
    TGGACTGAAC TGTTGCCTCC TCCTCCGGTT AATCCGCCGC CATGTCAAGA ATACACCCTG 
    GTGATGGACG ACAGTTACGA CACAGACCTG CAGTGCCCTT TGCCGCCGTC TCGGATGTAT 
    CTGCAACCTG ACGAGTTGGA AGAGGAGGAG GACGAGGTGG AGCTGGAGCG TGGACCTACA 
    CCTCCTGTAC GGGGAGCTGC TTCTTCACCT GCTGCCGTGT CCTACAGCCA CCAGTCCACC 
    GCAACCCTAA CCCCGTCCCC TCAGGAGGAG ATGCATCCCA TGCTGCAGGA CAACCCTGAT 
    CTACATGAGC GCAGGAGACT GTTAGTGAGT CCACCTCCTC CACCACGACC TCTCTCTCCT 
    CCACACACAT ACGGCTACAT CTCCAGTCCC CTCGGCCTGG ACACGGACGG CCTAGAGGAA 
    GAGGATGATG ATGAAGAAGA GGGTGACGAA ACGGACGCAG AGGTAGCTCG AGTCCACCAG 
    CACCACATGA TGCACCCTCA CCAGCAGCAC TTGCCCCAGC AGAGGGCTCT CCTGCGGGGT 
    CTGGAACAAA CCCCGGCCTC CAGCACCTGT GACCTGGAGA GCTCTGTGAC TGGGTCTATG 
    ATTAACGGAT GGGGTTCGGC TTCAGAAGAG GACAACGCCT CGTCAGGCCG TTCCAGTGCA 
    GTCAGCTCAT CGGATGGATC GTTCTTTACC GATGCCGATT TCGCCCAGGC TGTGGCAGCT 
    GCGGCTGAAT ATGCTGGTCT TCGAGTGGCA AAGCATCCTG GGAAAGGACA TCATCAGCTT 
    CCAGTGTCTG GAGCAGCACG GAAATACCAA AACCTCCCTG CGGGACACCG TCCAGGCAGC 
    CCAGTGTCCA CAGACAGTAA CGTTAGCGCA GCCCCGGGTC AGAGGAGGCC CCTGAAGAGG 
    CAAAAACAAC ATCCAGCTGC TCAGACAGGC TACCAGCGCC GAGAGGCCTA CACAGAGGAT 
    CTCCCCCCTC CTCCGATTCC TCCTCCAGCC ATGCTGAAGT CTCCAACACA CCCGGCCAAA 
    ACCATCCCCG AGGGTCCACG GGGGGCCATG TCGCCAAGAT CCAGCTCTGC GGATGGCAAG 
    GACAGGCGAA CGGGTGCTGG CGGGCCCAGG GTCAGGGACG GTTCAGACAC TCGGACCAGC 
    TCTTCAGAGC GGAGGGATGG CCAAGATGTA CAGAGGGGTC AGAGAACGGG CAAGAATAGC 
    AAACAAGATG AAGCCGTGAA GAGCCGCCAG CACCCAGGCT CAGAGGACAT CCTACCTTAC 
    AGTCGTCCTT CTTTCCCAGC CGTCCACAGT CCTCGAGGAG AACGGGACCC CAGTTCCTCC 
    AGTTCACTGT CCTCTCGGGG GTCTGGCGGG CGACGGAGAG GTGAAGGGCC ACCAGGGGCC 
    CGCAGAAACC CTGCAGACAT TGGGCTAAAC ACCATAGGAG CCTTCCAGAC TGGAGGGGAC 
    GAGCTTGAGA TGGTGGAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( XM_017359212.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214701.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID ODa55063
    Clone ID Related Accession (Same CDS sequence) XM_017359212.1
    Accession Version XM_017359212.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product roundabout homolog 1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTGGCAG CGGGGGAGCC AGCTGTAATG GAGTGCCAGC CGCCCCGAGG ACACCCAGAG 
    CCCACCATCT CCTGGAAGAA AGATGGGGTC AATATAGACG ACAGAGACGA GAGGATAACG 
    ATACGAGGAG GAAAGCTCAT GATCACCAAC ACCAGAAAAA CGGACGCGGG GAAGTACGTG 
    TGCGTGGGAA CCAACATGAT GGGGGAACGA GAGAGCGAGA TCGCGGAGTT GACTGTTCTC 
    GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT 
    GAGTTCAGAT GTGAAGCTCA AGGAGACCCC GTGCCCACCA TCCGATGGAG GAAAGAGGAC 
    GGAGAACTGC CCAAGGGAAG GTATGAGATC TGGGAGGACC ACACTCTCAA ACTGAAGAGG 
    CTGACTTCAG GAGACGCCGG TACTTACACC TGTCAGGCGG AGAACATGAT GGGCAAGACG 
    GAGGCCAGCG CCACCCTCAC CGTCCACGTT GTGTCTGTTC CTCCAGTGTT TGTGGTCCGA 
    CCCAGGAATC AGGTGGCGGG TATGGGCCGC ACCGTCACCT TTCAGTGTGA AGCCACAGGA 
    AGTCCTCAGC CTGCAATATT CTGGCAGAAA GAAGGCAGTC AGAATCTCCT GTTCTCGTCT 
    CAGCCTCCTC CTCCGTCCAG CAGATTCTCT GTGTCTCAGA CTGGAGATCT GACCATCACA 
    GCAGTTGAGC GTTCAGATGG AGGATATTAT AGCTGTCAGG CTCTCAACAT CGCTGGCAGT 
    GTGATCACCA AAGCCCTGCT GGAGGTTACA GACGTGATTT CAGACAGGCC TCCACCAATC 
    GTCCGTCAGG GTCCAACCAA CCAGACTGTG GCAGTGGACG GGACGGTGGT TCTGAGCTGC 
    CAAGCCACCG GGACACCGAT TCCTACCATC TTATGGAGGA AAGATGGAGT TCTGGTGTCC 
    ACACATGATT CCCGACTCAA GCAGCTGGAC ACTGGAGCCC TGCAGATTCG ATACGCAAAG 
    CTGGGCGACA CTGGCATGTA CACCTGCACG GCTTCCACCC CGAGCGGAGA GGCGTCCTGG 
    AAGGCCTACC TGGAGGTGCA AGAGTTTGGT GCTCCAGTTC AACCGGGCCG TCCCACAGAT 
    CCCAACCTCA TCCCCAGCGC CCCATCTAAA CCAGACGTCA CGGATGTCAG CCGCACCTCC 
    GTGTCTCTTT CCTGGAAGCC GAATCTGAAC GCTGGAGCCA CACCCACCTC ATACGTCATT 
    GAGGCCTTCA GCCATGCAGC AGGCAGCAGC TGGCAGACGC TCGCCGATCA TGTGAAGACG 
    GAAAGCTTCG TGCTTAAGGG TCTGAAGCCA AGTGCCGTCT ACCTGTTTCT GGTGAGAGCG 
    GCCAATGCGT ACGGCCTGAG CGACCCCAGT CCCATCAGCG ACGCTGTCAA AACACAAGAC 
    GTCCCACCAA CCAGCCAGGG AGTGGAGCAT GGCCAGATCC AGAGAGAACT GGGAGATGTC 
    GTCATTCACC TTCACAATCC CACAATTCTC TCATCGTCTT CCGTCAGAGT CCAGTGGACT 
    GTGGAGCAGC AGTCTCAGTA TATTCAGGGC TATAAGGTGA TGTACCGTCC GTCTCCAGAA 
    GGCGCTCCGC AGCGAGTGGA TTGGGCCATG TTTGAGGTCG GAGCCCCGGG GGAGCACAGC 
    GCTGTCGTCA CCCAGCTCAA GAAGGGCATC ACCTATGAGT TCAAAGTGCG CCCTTTCTTC 
    AATGAGTTCC AGGGCACCGA CAGCGACATC AAAGTGGGAA AGACCCTGGA GGAGGCTCCC 
    AGCGCTGCTC CCAGAGGCGT CACCGTAACA GGAAGTGGAG ACAACGGCAC AGCAGTCCTG 
    GTCGCCTGGC AACCGCCGCC AGAGGAGGAG CAGAACGGCG TTGTGCAGGA GTACAAGATC 
    TGGTGTCTTG GTAATGAAAG CCGATACCAC ATCAACCGCA CAGTGGATGG TTCTACACAC 
    TCGGTGTTGA TTCCTGGTCT GGTGGCAGGA GTCACATACA GGCTAGAGGT GGCAGCAGGC 
    ACTGGGGCCG GACCCGGGGT CAAGAGCGAC CCTACGTCCT TCCAGCTTGA CTCTTCTGGC 
    CGTGTTCTCC CGCCGCCGCT GGACGACGAT AACACCATTT CCCAGCAGAT CTCAGACGTG 
    GTGCGTCAGC CGGCTTTCAT CGCGGGCATC GGCGCCGCCT GCTGGATCAT CCTGATGGTG 
    TTCAGCATCT GGCTATACCG CCACCGCAAG AAGAGGAACG GCCTCAGCAG CAGCTACGCC 
    GGCATCCGCA AAGTGCCCTC CTTCACCTTC ACCCCCACAG TGGCCTATCA GAGAGGCGGA 
    GAGGCCGTCA GCAGTGCAGG AAGGCCGGGT TTGTTGAATA TAGGTGAACC AGCTACACAG 
    CCCTGGCTTG CGGACACCTG GCCCAACAGC TGCTCCAATC ACAATGACTG CACTATTAAC 
    TGCTGCACCG GCAGCAATGG AAACAGCGAC AGCAACCTGG CAACTTACAG CCGCCCAGCC 
    GACTGCATCG CCAACTACAG CAACCAGATG GACAACAAGG CCACCAACCT GCTGGCTCCA 
    GATACTGGGC TTTACAGCGA CGTTGACCTT TCAAACAAGA TCAACGAGAT GAAGACCTTC 
    AACAGCCCCA ACCTGAAGGA CGGGCGTTTT ATGGGGCCTG GTGGTCAACC CACACCCTAC 
    GCCACCACTC AGCTCATTCA ATCCAGCCTG GCCAACAACA ACATGAACAG CAGCAGTGGC 
    GGAAGCGGAG GAGGAAGCGC TGACTTTAAT GAGAAGCAGA GCTGGAAGAG CAGCCAGGGA 
    ACACCTCAGA AACAGGCCGA CATCAACACA CCACTGCAGT ATAACGTCAT GGAACAAAAC 
    AAACTCAACA AGGACCACTA CCGAGGTGAG GGTCCCATCT CAGTCACCAT CCCTTATAAC 
    CAGGCCCAGG ACGTCCTCAC AGGAGAAACC TACAGCAGCT CGGACAGAGG GAGCAGCACC 
    TCTGGAAGTC AAGGGCAGAA AAAGGGTGGA CGAACAACTA AACAAGCCAA ACAGAATCCA 
    ATGAATTGGA CTGAACTGTT GCCTCCTCCT CCGGTTAATC CGCCGCCATG TCAAGAATAC 
    ACCCTGGTGA TGGACGACAG TTACGACACA GACCTGCAGT GCCCTTTGCC GCCGTCTCGG 
    ATGTATCTGC AACCTGACGA GTTGGAAGAG GAGGAGGACG AGGTGGAGCT GGAGCGTGGA 
    CCTACACCTC CTGTACGGGG AGCTGCTTCT TCACCTGCTG CCGTGTCCTA CAGCCACCAG 
    TCCACCGCAA CCCTAACCCC GTCCCCTCAG GAGGAGATGC ATCCCATGCT GCAGGACAAC 
    CCTGATCTAC ATGAGCGCAG GAGACTGTTA GTGAGTCCAC CTCCTCCACC ACGACCTCTC 
    TCTCCTCCAC ACACATACGG CTACATCTCC AGTCCCCTCG GCCTGGACAC GGACGGCCTA 
    GAGGAAGAGG ATGATGATGA AGAAGAGGGT GACGAAACGG ACGCAGAGGT AGCTCGAGTC 
    CACCAGCACC ACATGATGCA CCCTCACCAG CAGCACTTGC CCCAGCAGAG GGCTCTCCTG 
    CGGGGTCTGG AACAAACCCC GGCCTCCAGC ACCTGTGACC TGGAGAGCTC TGTGACTGGG 
    TCTATGATTA ACGGATGGGG TTCGGCTTCA GAAGAGGACA ACGCCTCGTC AGGCCGTTCC 
    AGTGCAGTCA GCTCATCGGA TGGATCGTTC TTTACCGATG CCGATTTCGC CCAGGCTGTG 
    GCAGCTGCGG CTGAATATGC TGGTCTTCGA GTGGCAAAGC ATCCTGGGAA AGGACATCAT 
    CAGCTTCCAG TGTCTGGAGC AGCACGGAAA TACCAAAACC TCCCTGCGGG ACACCGTCCA 
    GGCAGCCCAG TGTCCACAGA CAGTAACGTT AGCGCAGCCC CGGGTCAGAG GAGGCCCCTG 
    AAGAGGCAAA AACAACATCC AGCTGCTCAG ACAGGCTACC AGCGCCGAGA GGCCTACACA 
    GAGGATCTCC CCCCTCCTCC GATTCCTCCT CCAGCCATGC TGAAGTCTCC AACACACCCG 
    GCCAAAACCA TCCCCGAGGG TCCACGGGGG GCCATGTCGC CAAGATCCAG CTCTGCGGAT 
    GGCAAGGACA GGCGAACGGG TGCTGGCGGG CCCAGGGTCA GGGACGGTTC AGACACTCGG 
    ACCAGCTCTT CAGAGCGGAG GGATGGCCAA GATGTACAGA GGGGTCAGAG AACGGGCAAG 
    AATAGCAAAC AAGATGAAGC CGTGAAGAGC CGCCAGCACC CAGGCTCAGA GGACATCCTA 
    CCTTACAGTC GTCCTTCTTT CCCAGCCGTC CACAGTCCTC GAGGAGAACG GGACCCCAGT 
    TCCTCCAGTT CACTGTCCTC TCGGGGGTCT GGCGGGCGAC GGAGAGGTGA AGGGCCACCA 
    GGGGCCCGCA GAAACCCTGC AGACATTGGG CTAAACACCA TAGGAGCCTT CCAGACTGGA 
    GGGGACGAGC TTGAGATGGT GGAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214701.1
    CDS213..4742
    Translation

    Target ORF information:

    RefSeq Version XM_017359212.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017359212.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTGGCAG CGGGGGAGCC AGCTGTAATG GAGTGCCAGC CGCCCCGAGG ACACCCAGAG 
    CCCACCATCT CCTGGAAGAA AGATGGGGTC AATATAGACG ACAGAGACGA GAGGATAACG 
    ATACGAGGAG GAAAGCTCAT GATCACCAAC ACCAGAAAAA CGGACGCGGG GAAGTACGTG 
    TGCGTGGGAA CCAACATGAT GGGGGAACGA GAGAGCGAGA TCGCGGAGTT GACTGTTCTC 
    GAGAGGCCCA GTTTCTTGCG AAAGCCGAGC AGCCAGTCCG TCCTTGTGGA TCAGAGCGTT 
    GAGTTCAGAT GTGAAGCTCA AGGAGACCCC GTGCCCACCA TCCGATGGAG GAAAGAGGAC 
    GGAGAACTGC CCAAGGGAAG GTATGAGATC TGGGAGGACC ACACTCTCAA ACTGAAGAGG 
    CTGACTTCAG GAGACGCCGG TACTTACACC TGTCAGGCGG AGAACATGAT GGGCAAGACG 
    GAGGCCAGCG CCACCCTCAC CGTCCACGTT GTGTCTGTTC CTCCAGTGTT TGTGGTCCGA 
    CCCAGGAATC AGGTGGCGGG TATGGGCCGC ACCGTCACCT TTCAGTGTGA AGCCACAGGA 
    AGTCCTCAGC CTGCAATATT CTGGCAGAAA GAAGGCAGTC AGAATCTCCT GTTCTCGTCT 
    CAGCCTCCTC CTCCGTCCAG CAGATTCTCT GTGTCTCAGA CTGGAGATCT GACCATCACA 
    GCAGTTGAGC GTTCAGATGG AGGATATTAT AGCTGTCAGG CTCTCAACAT CGCTGGCAGT 
    GTGATCACCA AAGCCCTGCT GGAGGTTACA GACGTGATTT CAGACAGGCC TCCACCAATC 
    GTCCGTCAGG GTCCAACCAA CCAGACTGTG GCAGTGGACG GGACGGTGGT TCTGAGCTGC 
    CAAGCCACCG GGACACCGAT TCCTACCATC TTATGGAGGA AAGATGGAGT TCTGGTGTCC 
    ACACATGATT CCCGACTCAA GCAGCTGGAC ACTGGAGCCC TGCAGATTCG ATACGCAAAG 
    CTGGGCGACA CTGGCATGTA CACCTGCACG GCTTCCACCC CGAGCGGAGA GGCGTCCTGG 
    AAGGCCTACC TGGAGGTGCA AGAGTTTGGT GCTCCAGTTC AACCGGGCCG TCCCACAGAT 
    CCCAACCTCA TCCCCAGCGC CCCATCTAAA CCAGACGTCA CGGATGTCAG CCGCACCTCC 
    GTGTCTCTTT CCTGGAAGCC GAATCTGAAC GCTGGAGCCA CACCCACCTC ATACGTCATT 
    GAGGCCTTCA GCCATGCAGC AGGCAGCAGC TGGCAGACGC TCGCCGATCA TGTGAAGACG 
    GAAAGCTTCG TGCTTAAGGG TCTGAAGCCA AGTGCCGTCT ACCTGTTTCT GGTGAGAGCG 
    GCCAATGCGT ACGGCCTGAG CGACCCCAGT CCCATCAGCG ACGCTGTCAA AACACAAGAC 
    GTCCCACCAA CCAGCCAGGG AGTGGAGCAT GGCCAGATCC AGAGAGAACT GGGAGATGTC 
    GTCATTCACC TTCACAATCC CACAATTCTC TCATCGTCTT CCGTCAGAGT CCAGTGGACT 
    GTGGAGCAGC AGTCTCAGTA TATTCAGGGC TATAAGGTGA TGTACCGTCC GTCTCCAGAA 
    GGCGCTCCGC AGCGAGTGGA TTGGGCCATG TTTGAGGTCG GAGCCCCGGG GGAGCACAGC 
    GCTGTCGTCA CCCAGCTCAA GAAGGGCATC ACCTATGAGT TCAAAGTGCG CCCTTTCTTC 
    AATGAGTTCC AGGGCACCGA CAGCGACATC AAAGTGGGAA AGACCCTGGA GGAGGCTCCC 
    AGCGCTGCTC CCAGAGGCGT CACCGTAACA GGAAGTGGAG ACAACGGCAC AGCAGTCCTG 
    GTCGCCTGGC AACCGCCGCC AGAGGAGGAG CAGAACGGCG TTGTGCAGGA GTACAAGATC 
    TGGTGTCTTG GTAATGAAAG CCGATACCAC ATCAACCGCA CAGTGGATGG TTCTACACAC 
    TCGGTGTTGA TTCCTGGTCT GGTGGCAGGA GTCACATACA GGCTAGAGGT GGCAGCAGGC 
    ACTGGGGCCG GACCCGGGGT CAAGAGCGAC CCTACGTCCT TCCAGCTTGA CTCTTCTGGC 
    CGTGTTCTCC CGCCGCCGCT GGACGACGAT AACACCATTT CCCAGCAGAT CTCAGACGTG 
    GTGCGTCAGC CGGCTTTCAT CGCGGGCATC GGCGCCGCCT GCTGGATCAT CCTGATGGTG 
    TTCAGCATCT GGCTATACCG CCACCGCAAG AAGAGGAACG GCCTCAGCAG CAGCTACGCC 
    GGCATCCGCA AAGTGCCCTC CTTCACCTTC ACCCCCACAG TGGCCTATCA GAGAGGCGGA 
    GAGGCCGTCA GCAGTGCAGG AAGGCCGGGT TTGTTGAATA TAGGTGAACC AGCTACACAG 
    CCCTGGCTTG CGGACACCTG GCCCAACAGC TGCTCCAATC ACAATGACTG CACTATTAAC 
    TGCTGCACCG GCAGCAATGG AAACAGCGAC AGCAACCTGG CAACTTACAG CCGCCCAGCC 
    GACTGCATCG CCAACTACAG CAACCAGATG GACAACAAGG CCACCAACCT GCTGGCTCCA 
    GATACTGGGC TTTACAGCGA CGTTGACCTT TCAAACAAGA TCAACGAGAT GAAGACCTTC 
    AACAGCCCCA ACCTGAAGGA CGGGCGTTTT ATGGGGCCTG GTGGTCAACC CACACCCTAC 
    GCCACCACTC AGCTCATTCA ATCCAGCCTG GCCAACAACA ACATGAACAG CAGCAGTGGC 
    GGAAGCGGAG GAGGAAGCGC TGACTTTAAT GAGAAGCAGA GCTGGAAGAG CAGCCAGGGA 
    ACACCTCAGA AACAGGCCGA CATCAACACA CCACTGCAGT ATAACGTCAT GGAACAAAAC 
    AAACTCAACA AGGACCACTA CCGAGGTGAG GGTCCCATCT CAGTCACCAT CCCTTATAAC 
    CAGGCCCAGG ACGTCCTCAC AGGAGAAACC TACAGCAGCT CGGACAGAGG GAGCAGCACC 
    TCTGGAAGTC AAGGGCAGAA AAAGGGTGGA CGAACAACTA AACAAGCCAA ACAGAATCCA 
    ATGAATTGGA CTGAACTGTT GCCTCCTCCT CCGGTTAATC CGCCGCCATG TCAAGAATAC 
    ACCCTGGTGA TGGACGACAG TTACGACACA GACCTGCAGT GCCCTTTGCC GCCGTCTCGG 
    ATGTATCTGC AACCTGACGA GTTGGAAGAG GAGGAGGACG AGGTGGAGCT GGAGCGTGGA 
    CCTACACCTC CTGTACGGGG AGCTGCTTCT TCACCTGCTG CCGTGTCCTA CAGCCACCAG 
    TCCACCGCAA CCCTAACCCC GTCCCCTCAG GAGGAGATGC ATCCCATGCT GCAGGACAAC 
    CCTGATCTAC ATGAGCGCAG GAGACTGTTA GTGAGTCCAC CTCCTCCACC ACGACCTCTC 
    TCTCCTCCAC ACACATACGG CTACATCTCC AGTCCCCTCG GCCTGGACAC GGACGGCCTA 
    GAGGAAGAGG ATGATGATGA AGAAGAGGGT GACGAAACGG ACGCAGAGGT AGCTCGAGTC 
    CACCAGCACC ACATGATGCA CCCTCACCAG CAGCACTTGC CCCAGCAGAG GGCTCTCCTG 
    CGGGGTCTGG AACAAACCCC GGCCTCCAGC ACCTGTGACC TGGAGAGCTC TGTGACTGGG 
    TCTATGATTA ACGGATGGGG TTCGGCTTCA GAAGAGGACA ACGCCTCGTC AGGCCGTTCC 
    AGTGCAGTCA GCTCATCGGA TGGATCGTTC TTTACCGATG CCGATTTCGC CCAGGCTGTG 
    GCAGCTGCGG CTGAATATGC TGGTCTTCGA GTGGCAAAGC ATCCTGGGAA AGGACATCAT 
    CAGCTTCCAG TGTCTGGAGC AGCACGGAAA TACCAAAACC TCCCTGCGGG ACACCGTCCA 
    GGCAGCCCAG TGTCCACAGA CAGTAACGTT AGCGCAGCCC CGGGTCAGAG GAGGCCCCTG 
    AAGAGGCAAA AACAACATCC AGCTGCTCAG ACAGGCTACC AGCGCCGAGA GGCCTACACA 
    GAGGATCTCC CCCCTCCTCC GATTCCTCCT CCAGCCATGC TGAAGTCTCC AACACACCCG 
    GCCAAAACCA TCCCCGAGGG TCCACGGGGG GCCATGTCGC CAAGATCCAG CTCTGCGGAT 
    GGCAAGGACA GGCGAACGGG TGCTGGCGGG CCCAGGGTCA GGGACGGTTC AGACACTCGG 
    ACCAGCTCTT CAGAGCGGAG GGATGGCCAA GATGTACAGA GGGGTCAGAG AACGGGCAAG 
    AATAGCAAAC AAGATGAAGC CGTGAAGAGC CGCCAGCACC CAGGCTCAGA GGACATCCTA 
    CCTTACAGTC GTCCTTCTTT CCCAGCCGTC CACAGTCCTC GAGGAGAACG GGACCCCAGT 
    TCCTCCAGTT CACTGTCCTC TCGGGGGTCT GGCGGGCGAC GGAGAGGTGA AGGGCCACCA 
    GGGGCCCGCA GAAACCCTGC AGACATTGGG CTAAACACCA TAGGAGCCTT CCAGACTGGA 
    GGGGACGAGC TTGAGATGGT GGAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( NM_001309824.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001296753.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID ODa39335
    Clone ID Related Accession (Same CDS sequence) NM_001309824.2 , NM_001309824.1
    Accession Version NM_001309824.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-18
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 isoform 3 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU694228.9, CU695212.10, CU855934.6 and FP015879.3. On Aug 3, 2018 this sequence version replaced NM_001309824.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTCCTCCAG TGTTTGTGGT CCGACCCAGG 
    AATCAGGTGG CGGGTATGGG CCGCACCGTC ACCTTTCAGT GTGAAGCCAC AGGAAGTCCT 
    CAGCCTGCAA TATTCTGGCA GAAAGAAGGC AGTCAGAATC TCCTGTTCTC GTCTCAGCCT 
    CCTCCTCCGT CCAGCAGATT CTCTGTGTCT CAGACTGGAG ATCTGACCAT CACAGCAGTT 
    GAGCGTTCAG ATGGAGGATA TTATAGCTGT CAGGCTCTCA ACATCGCTGG CAGTGTGATC 
    ACCAAAGCCC TGCTGGAGGT TACAGACGTG ATTTCAGACA GGCCTCCACC AATCGTCCGT 
    CAGGGTCCAA CCAACCAGAC TGTGGCAGTG GACGGGACGG TGGTTCTGAG CTGCCAAGCC 
    ACCGGGACAC CGATTCCTAC CATCTTATGG AGGAAAGATG GAGTTCTGGT GTCCACACAT 
    GATTCCCGAC TCAAGCAGCT GGACACTGGA GCCCTGCAGA TTCGATACGC AAAGCTGGGC 
    GACACTGGCA TGTACACCTG CACGGCTTCC ACCCCGAGCG GAGAGGCGTC CTGGAAGGCC 
    TACCTGGAGG TGCAAGAGTT TGGTGCTCCA GTTCAACCGG GCCGTCCCAC AGATCCCAAC 
    CTCATCCCCA GCGCCCCATC TAAACCAGAC GTCACGGATG TCAGCCGCAC CTCCGTGTCT 
    CTTTCCTGGA AGCCGAATCT GAACGCTGGA GCCACACCCA CCTCATACGT CATTGAGGCC 
    TTCAGCCATG CAGCAGGCAG CAGCTGGCAG ACGCTCGCCG ATCATGTGAA GACGGAAAGC 
    TTCGTGCTTA AGGGTCTGAA GCCAAGTGCC GTCTACCTGT TTCTGGTGAG AGCGGCCAAT 
    GCGTACGGCC TGAGCGACCC CAGTCCCATC AGCGACGCTG TCAAAACACA AGACGTCCCA 
    CCAACCAGCC AGGGAGTGGA GCATGGCCAG ATCCAGAGAG AACTGGGAGA TGTCGTCATT 
    CACCTTCACA ATCCCACAAT TCTCTCATCG TCTTCCGTCA GAGTCCAGTG GACTGTGGAG 
    CAGCAGTCTC AGTATATTCA GGGCTATAAG GTGATGTACC GTCCGTCTCC AGAAGGCGCT 
    CCGCAGCGAG TGGATTGGGC CATGTTTGAG GTCGGAGCCC CGGGGGAGCA CAGCGCTGTC 
    GTCACCCAGC TCAAGAAGGG CATCACCTAT GAGTTCAAAG TGCGCCCTTT CTTCAATGAG 
    TTCCAGGGCA CCGACAGCGA CATCAAAGTG GGAAAGACCC TGGAGGAGGC TCCCAGCGCT 
    GCTCCCAGAG GCGTCACCGT AACAGGAAGT GGAGACAACG GCACAGCAGT CCTGGTCGCC 
    TGGCAACCGC CGCCAGAGGA GGAGCAGAAC GGCGTTGTGC AGGAGTACAA GATCTGGTGT 
    CTTGGTAATG AAAGCCGATA CCACATCAAC CGCACAGTGG ATGGTTCTAC ACACTCGGTG 
    TTGATTCCTG GTCTGGTGGC AGGAGTCACA TACAGGCTAG AGGTGGCAGC AGGCACTGGG 
    GCCGGACCCG GGGTCAAGAG CGACCCTACG TCCTTCCAGC TTGACTCTTC TGGCCGTGTT 
    CTCCCGCCGC CGCTGGACGA CGATAACACC ATTTCCCAGC AGATCTCAGA CGTGGTGCGT 
    CAGCCGGCTT TCATCGCGGG CATCGGCGCC GCCTGCTGGA TCATCCTGAT GGTGTTCAGC 
    ATCTGGCTAT ACCGCCACCG CAAGAAGAGG AACGGCCTCA GCAGCAGCTA CGCCGGCATC 
    CGCAAAGTGC CCTCCTTCAC CTTCACCCCC ACAGTGGCCT ATCAGAGAGG CGGAGAGGCC 
    GTCAGCAGTG CAGGAAGGCC GGGTTTGTTG AATATAGGTG AACCAGCTAC ACAGCCCTGG 
    CTTGCGGACA CCTGGCCCAA CAGCTGCTCC AATCACAATG ACTGCACTAT TAACTGCTGC 
    ACCGGCAGCA ATGGAAACAG CGACAGCAAC CTGGCAACTT ACAGCCGCCC AGCCGACTGC 
    ATCGCCAACT ACAGCAACCA GATGGACAAC AAGGCCACCA ACCTGCTGGC TCCAGATACT 
    GGGCTTTACA GCGACGTTGA CCTTTCAAAC AAGATCAACG AGATGAAGAC CTTCAACAGC 
    CCCAACCTGA AGGACGGGCG TTTTATGGGG CCTGGTGGTC AACCCACACC CTACGCCACC 
    ACTCAGCTCA TTCAATCCAG CCTGGCCAAC AACAACATGA ACAGCAGCAG TGGCGGAAGC 
    GGAGGAGGAA GCGCTGACTT TAATGAGAAG CAGAGCTGGA AGAGCAGCCA GGGAACACCT 
    CAGAAACAGG CCGACATCAA CACACCACTG CAGTATAACG TCATGGAACA AAACAAACTC 
    AACAAGGACC ACTACCGAGG TGAGGGTCCC ATCTCAGTCA CCATCCCTTA TAACCAGGCC 
    CAGGACGTCC TCACAGGAGA AACCTACAGC AGCTCGGACA GAGGGAGCAG CACCTCTGGA 
    AGTCAAGGGC AGAAAAAGGG TGGACGAACA ACTAAACAAG CCAAACAGAA TCCAATGAAT 
    TGGACTGAAC TGTTGCCTCC TCCTCCGGTT AATCCGCCGC CATGTCAAGA ATACACCCTG 
    GTGATGGACG ACAGTTACGA CACAGACCTG CAGTGCCCTT TGCCGCCGTC TCGGATGTAT 
    CTGCAACCTG ACGAGTTGGA AGAGGAGGAG GACGAGGTGG AGCTGGAGCG TGGACCTACA 
    CCTCCTGTAC GGGGAGCTGC TTCTTCACCT GCTGCCGTGT CCTACAGCCA CCAGTCCACC 
    GCAACCCTAA CCCCGTCCCC TCAGGAGGAG ATGCATCCCA TGCTGCAGGA CAACCCTGAT 
    CTACATGAGC GCAGGAGACT GTTAGTGAGT CCACCTCCTC CACCACGACC TCTCTCTCCT 
    CCACACACAT ACGGCTACAT CTCCAGTCCC CTCGGCCTGG ACACGGACGG CCTAGAGGAA 
    GAGGATGATG ATGAAGAAGA GGGTGACGAA ACGGACGCAG AGGTAGCTCG AGTCCACCAG 
    CACCACATGA TGCACCCTCA CCAGCAGCAC TTGCCCCAGC AGAGGGCTCT CCTGCGGGGT 
    CTGGAACAAA CCCCGGCCTC CAGCACCTGT GACCTGGAGA GCTCTGTGAC TGGGTCTATG 
    ATTAACGGAT GGGGTTCGGC TTCAGAAGAG GACAACGCCT CGTCAGGCCG TTCCAGTGCA 
    GTCAGCTCAT CGGATGGATC GTTCTTTACC GATGCCGATT TCGCCCAGGC TGTGGCAGCT 
    GCGGCTGAAT ATGCTGGTCT TCGAGTGGCA AAGCATCCTG GGAAAGGACA TCATCAGCTT 
    CCAGTGTCTG GAGCACGGAA ATACCAAAAC CTCCCTGCGG GACACCGTCC AGGCAGCCCA 
    GTGTCCACAG ACAGTAACGT TAGCGCAGCC CCGGGTCAGA GGAGGCCCCT GAAGAGGCAA 
    AAACAACATC CAGCTGCTCA GACAGGCTAC CAGCGCCGAG AGGCCTACAC AGAGGATCTC 
    CCCCCTCCTC CGATTCCTCC TCCAGCCATG CTGAAGTCTC CAACACACCC GGCCAAAACC 
    ATCCCCGAGG GTCCACGGGG GGCCATGTCG CCAAGATCCA GCTCTGCGGA TGGCAAGGAC 
    AGGCGAACGG GTGCTGGCGG GCCCAGGGTC AGGGACGGTT CAGACACTCG GACCAGCTCT 
    TCAGAGCGGA GGGATGGCCA AGATGTACAG AGGGGTCAGA GAACGGGCAA GAATAGCAAA 
    CAAGATGAAG CCGTGAAGAG CCGCCAGCAC CCAGGCTCAG AGGACATCCT ACCTTACAGT 
    CGTCCTTCTT TCCCAGCCGT CCACAGTCCT CGAGGAGAAC GGGACCCCAG TTCCTCCAGT 
    TCACTGTCCT CTCGGGGGTC TGGCGGGCGA CGGAGAGGTG AAGGGCCACC AGGGGCCCGC 
    AGAAACCCTG CAGACATTGG GCTAAACACC ATAGGAGCCT TCCAGACTGG AGGGGACGAG 
    CTTGAGATGG TGGAGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001296753.1
    CDS743..5683
    Translation

    Target ORF information:

    RefSeq Version NM_001309824.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001309824.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTCCTCCAG TGTTTGTGGT CCGACCCAGG 
    AATCAGGTGG CGGGTATGGG CCGCACCGTC ACCTTTCAGT GTGAAGCCAC AGGAAGTCCT 
    CAGCCTGCAA TATTCTGGCA GAAAGAAGGC AGTCAGAATC TCCTGTTCTC GTCTCAGCCT 
    CCTCCTCCGT CCAGCAGATT CTCTGTGTCT CAGACTGGAG ATCTGACCAT CACAGCAGTT 
    GAGCGTTCAG ATGGAGGATA TTATAGCTGT CAGGCTCTCA ACATCGCTGG CAGTGTGATC 
    ACCAAAGCCC TGCTGGAGGT TACAGACGTG ATTTCAGACA GGCCTCCACC AATCGTCCGT 
    CAGGGTCCAA CCAACCAGAC TGTGGCAGTG GACGGGACGG TGGTTCTGAG CTGCCAAGCC 
    ACCGGGACAC CGATTCCTAC CATCTTATGG AGGAAAGATG GAGTTCTGGT GTCCACACAT 
    GATTCCCGAC TCAAGCAGCT GGACACTGGA GCCCTGCAGA TTCGATACGC AAAGCTGGGC 
    GACACTGGCA TGTACACCTG CACGGCTTCC ACCCCGAGCG GAGAGGCGTC CTGGAAGGCC 
    TACCTGGAGG TGCAAGAGTT TGGTGCTCCA GTTCAACCGG GCCGTCCCAC AGATCCCAAC 
    CTCATCCCCA GCGCCCCATC TAAACCAGAC GTCACGGATG TCAGCCGCAC CTCCGTGTCT 
    CTTTCCTGGA AGCCGAATCT GAACGCTGGA GCCACACCCA CCTCATACGT CATTGAGGCC 
    TTCAGCCATG CAGCAGGCAG CAGCTGGCAG ACGCTCGCCG ATCATGTGAA GACGGAAAGC 
    TTCGTGCTTA AGGGTCTGAA GCCAAGTGCC GTCTACCTGT TTCTGGTGAG AGCGGCCAAT 
    GCGTACGGCC TGAGCGACCC CAGTCCCATC AGCGACGCTG TCAAAACACA AGACGTCCCA 
    CCAACCAGCC AGGGAGTGGA GCATGGCCAG ATCCAGAGAG AACTGGGAGA TGTCGTCATT 
    CACCTTCACA ATCCCACAAT TCTCTCATCG TCTTCCGTCA GAGTCCAGTG GACTGTGGAG 
    CAGCAGTCTC AGTATATTCA GGGCTATAAG GTGATGTACC GTCCGTCTCC AGAAGGCGCT 
    CCGCAGCGAG TGGATTGGGC CATGTTTGAG GTCGGAGCCC CGGGGGAGCA CAGCGCTGTC 
    GTCACCCAGC TCAAGAAGGG CATCACCTAT GAGTTCAAAG TGCGCCCTTT CTTCAATGAG 
    TTCCAGGGCA CCGACAGCGA CATCAAAGTG GGAAAGACCC TGGAGGAGGC TCCCAGCGCT 
    GCTCCCAGAG GCGTCACCGT AACAGGAAGT GGAGACAACG GCACAGCAGT CCTGGTCGCC 
    TGGCAACCGC CGCCAGAGGA GGAGCAGAAC GGCGTTGTGC AGGAGTACAA GATCTGGTGT 
    CTTGGTAATG AAAGCCGATA CCACATCAAC CGCACAGTGG ATGGTTCTAC ACACTCGGTG 
    TTGATTCCTG GTCTGGTGGC AGGAGTCACA TACAGGCTAG AGGTGGCAGC AGGCACTGGG 
    GCCGGACCCG GGGTCAAGAG CGACCCTACG TCCTTCCAGC TTGACTCTTC TGGCCGTGTT 
    CTCCCGCCGC CGCTGGACGA CGATAACACC ATTTCCCAGC AGATCTCAGA CGTGGTGCGT 
    CAGCCGGCTT TCATCGCGGG CATCGGCGCC GCCTGCTGGA TCATCCTGAT GGTGTTCAGC 
    ATCTGGCTAT ACCGCCACCG CAAGAAGAGG AACGGCCTCA GCAGCAGCTA CGCCGGCATC 
    CGCAAAGTGC CCTCCTTCAC CTTCACCCCC ACAGTGGCCT ATCAGAGAGG CGGAGAGGCC 
    GTCAGCAGTG CAGGAAGGCC GGGTTTGTTG AATATAGGTG AACCAGCTAC ACAGCCCTGG 
    CTTGCGGACA CCTGGCCCAA CAGCTGCTCC AATCACAATG ACTGCACTAT TAACTGCTGC 
    ACCGGCAGCA ATGGAAACAG CGACAGCAAC CTGGCAACTT ACAGCCGCCC AGCCGACTGC 
    ATCGCCAACT ACAGCAACCA GATGGACAAC AAGGCCACCA ACCTGCTGGC TCCAGATACT 
    GGGCTTTACA GCGACGTTGA CCTTTCAAAC AAGATCAACG AGATGAAGAC CTTCAACAGC 
    CCCAACCTGA AGGACGGGCG TTTTATGGGG CCTGGTGGTC AACCCACACC CTACGCCACC 
    ACTCAGCTCA TTCAATCCAG CCTGGCCAAC AACAACATGA ACAGCAGCAG TGGCGGAAGC 
    GGAGGAGGAA GCGCTGACTT TAATGAGAAG CAGAGCTGGA AGAGCAGCCA GGGAACACCT 
    CAGAAACAGG CCGACATCAA CACACCACTG CAGTATAACG TCATGGAACA AAACAAACTC 
    AACAAGGACC ACTACCGAGG TGAGGGTCCC ATCTCAGTCA CCATCCCTTA TAACCAGGCC 
    CAGGACGTCC TCACAGGAGA AACCTACAGC AGCTCGGACA GAGGGAGCAG CACCTCTGGA 
    AGTCAAGGGC AGAAAAAGGG TGGACGAACA ACTAAACAAG CCAAACAGAA TCCAATGAAT 
    TGGACTGAAC TGTTGCCTCC TCCTCCGGTT AATCCGCCGC CATGTCAAGA ATACACCCTG 
    GTGATGGACG ACAGTTACGA CACAGACCTG CAGTGCCCTT TGCCGCCGTC TCGGATGTAT 
    CTGCAACCTG ACGAGTTGGA AGAGGAGGAG GACGAGGTGG AGCTGGAGCG TGGACCTACA 
    CCTCCTGTAC GGGGAGCTGC TTCTTCACCT GCTGCCGTGT CCTACAGCCA CCAGTCCACC 
    GCAACCCTAA CCCCGTCCCC TCAGGAGGAG ATGCATCCCA TGCTGCAGGA CAACCCTGAT 
    CTACATGAGC GCAGGAGACT GTTAGTGAGT CCACCTCCTC CACCACGACC TCTCTCTCCT 
    CCACACACAT ACGGCTACAT CTCCAGTCCC CTCGGCCTGG ACACGGACGG CCTAGAGGAA 
    GAGGATGATG ATGAAGAAGA GGGTGACGAA ACGGACGCAG AGGTAGCTCG AGTCCACCAG 
    CACCACATGA TGCACCCTCA CCAGCAGCAC TTGCCCCAGC AGAGGGCTCT CCTGCGGGGT 
    CTGGAACAAA CCCCGGCCTC CAGCACCTGT GACCTGGAGA GCTCTGTGAC TGGGTCTATG 
    ATTAACGGAT GGGGTTCGGC TTCAGAAGAG GACAACGCCT CGTCAGGCCG TTCCAGTGCA 
    GTCAGCTCAT CGGATGGATC GTTCTTTACC GATGCCGATT TCGCCCAGGC TGTGGCAGCT 
    GCGGCTGAAT ATGCTGGTCT TCGAGTGGCA AAGCATCCTG GGAAAGGACA TCATCAGCTT 
    CCAGTGTCTG GAGCACGGAA ATACCAAAAC CTCCCTGCGG GACACCGTCC AGGCAGCCCA 
    GTGTCCACAG ACAGTAACGT TAGCGCAGCC CCGGGTCAGA GGAGGCCCCT GAAGAGGCAA 
    AAACAACATC CAGCTGCTCA GACAGGCTAC CAGCGCCGAG AGGCCTACAC AGAGGATCTC 
    CCCCCTCCTC CGATTCCTCC TCCAGCCATG CTGAAGTCTC CAACACACCC GGCCAAAACC 
    ATCCCCGAGG GTCCACGGGG GGCCATGTCG CCAAGATCCA GCTCTGCGGA TGGCAAGGAC 
    AGGCGAACGG GTGCTGGCGG GCCCAGGGTC AGGGACGGTT CAGACACTCG GACCAGCTCT 
    TCAGAGCGGA GGGATGGCCA AGATGTACAG AGGGGTCAGA GAACGGGCAA GAATAGCAAA 
    CAAGATGAAG CCGTGAAGAG CCGCCAGCAC CCAGGCTCAG AGGACATCCT ACCTTACAGT 
    CGTCCTTCTT TCCCAGCCGT CCACAGTCCT CGAGGAGAAC GGGACCCCAG TTCCTCCAGT 
    TCACTGTCCT CTCGGGGGTC TGGCGGGCGA CGGAGAGGTG AAGGGCCACC AGGGGCCCGC 
    AGAAACCCTG CAGACATTGG GCTAAACACC ATAGGAGCCT TCCAGACTGG AGGGGACGAG 
    CTTGAGATGG TGGAGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    robo1 ( NM_001309824.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001296753.1 Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), mRNA.
    Price & Availability↑
    CloneID ODa39335
    Clone ID Related Accession (Same CDS sequence) NM_001309824.2 , NM_001309824.1
    Accession Version NM_001309824.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-06
    Organism Danio rerio(zebrafish)
    Product roundabout homolog 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU694228.9, CU695212.10, CU855934.6 and FP015879.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTCCTCCAG TGTTTGTGGT CCGACCCAGG 
    AATCAGGTGG CGGGTATGGG CCGCACCGTC ACCTTTCAGT GTGAAGCCAC AGGAAGTCCT 
    CAGCCTGCAA TATTCTGGCA GAAAGAAGGC AGTCAGAATC TCCTGTTCTC GTCTCAGCCT 
    CCTCCTCCGT CCAGCAGATT CTCTGTGTCT CAGACTGGAG ATCTGACCAT CACAGCAGTT 
    GAGCGTTCAG ATGGAGGATA TTATAGCTGT CAGGCTCTCA ACATCGCTGG CAGTGTGATC 
    ACCAAAGCCC TGCTGGAGGT TACAGACGTG ATTTCAGACA GGCCTCCACC AATCGTCCGT 
    CAGGGTCCAA CCAACCAGAC TGTGGCAGTG GACGGGACGG TGGTTCTGAG CTGCCAAGCC 
    ACCGGGACAC CGATTCCTAC CATCTTATGG AGGAAAGATG GAGTTCTGGT GTCCACACAT 
    GATTCCCGAC TCAAGCAGCT GGACACTGGA GCCCTGCAGA TTCGATACGC AAAGCTGGGC 
    GACACTGGCA TGTACACCTG CACGGCTTCC ACCCCGAGCG GAGAGGCGTC CTGGAAGGCC 
    TACCTGGAGG TGCAAGAGTT TGGTGCTCCA GTTCAACCGG GCCGTCCCAC AGATCCCAAC 
    CTCATCCCCA GCGCCCCATC TAAACCAGAC GTCACGGATG TCAGCCGCAC CTCCGTGTCT 
    CTTTCCTGGA AGCCGAATCT GAACGCTGGA GCCACACCCA CCTCATACGT CATTGAGGCC 
    TTCAGCCATG CAGCAGGCAG CAGCTGGCAG ACGCTCGCCG ATCATGTGAA GACGGAAAGC 
    TTCGTGCTTA AGGGTCTGAA GCCAAGTGCC GTCTACCTGT TTCTGGTGAG AGCGGCCAAT 
    GCGTACGGCC TGAGCGACCC CAGTCCCATC AGCGACGCTG TCAAAACACA AGACGTCCCA 
    CCAACCAGCC AGGGAGTGGA GCATGGCCAG ATCCAGAGAG AACTGGGAGA TGTCGTCATT 
    CACCTTCACA ATCCCACAAT TCTCTCATCG TCTTCCGTCA GAGTCCAGTG GACTGTGGAG 
    CAGCAGTCTC AGTATATTCA GGGCTATAAG GTGATGTACC GTCCGTCTCC AGAAGGCGCT 
    CCGCAGCGAG TGGATTGGGC CATGTTTGAG GTCGGAGCCC CGGGGGAGCA CAGCGCTGTC 
    GTCACCCAGC TCAAGAAGGG CATCACCTAT GAGTTCAAAG TGCGCCCTTT CTTCAATGAG 
    TTCCAGGGCA CCGACAGCGA CATCAAAGTG GGAAAGACCC TGGAGGAGGC TCCCAGCGCT 
    GCTCCCAGAG GCGTCACCGT AACAGGAAGT GGAGACAACG GCACAGCAGT CCTGGTCGCC 
    TGGCAACCGC CGCCAGAGGA GGAGCAGAAC GGCGTTGTGC AGGAGTACAA GATCTGGTGT 
    CTTGGTAATG AAAGCCGATA CCACATCAAC CGCACAGTGG ATGGTTCTAC ACACTCGGTG 
    TTGATTCCTG GTCTGGTGGC AGGAGTCACA TACAGGCTAG AGGTGGCAGC AGGCACTGGG 
    GCCGGACCCG GGGTCAAGAG CGACCCTACG TCCTTCCAGC TTGACTCTTC TGGCCGTGTT 
    CTCCCGCCGC CGCTGGACGA CGATAACACC ATTTCCCAGC AGATCTCAGA CGTGGTGCGT 
    CAGCCGGCTT TCATCGCGGG CATCGGCGCC GCCTGCTGGA TCATCCTGAT GGTGTTCAGC 
    ATCTGGCTAT ACCGCCACCG CAAGAAGAGG AACGGCCTCA GCAGCAGCTA CGCCGGCATC 
    CGCAAAGTGC CCTCCTTCAC CTTCACCCCC ACAGTGGCCT ATCAGAGAGG CGGAGAGGCC 
    GTCAGCAGTG CAGGAAGGCC GGGTTTGTTG AATATAGGTG AACCAGCTAC ACAGCCCTGG 
    CTTGCGGACA CCTGGCCCAA CAGCTGCTCC AATCACAATG ACTGCACTAT TAACTGCTGC 
    ACCGGCAGCA ATGGAAACAG CGACAGCAAC CTGGCAACTT ACAGCCGCCC AGCCGACTGC 
    ATCGCCAACT ACAGCAACCA GATGGACAAC AAGGCCACCA ACCTGCTGGC TCCAGATACT 
    GGGCTTTACA GCGACGTTGA CCTTTCAAAC AAGATCAACG AGATGAAGAC CTTCAACAGC 
    CCCAACCTGA AGGACGGGCG TTTTATGGGG CCTGGTGGTC AACCCACACC CTACGCCACC 
    ACTCAGCTCA TTCAATCCAG CCTGGCCAAC AACAACATGA ACAGCAGCAG TGGCGGAAGC 
    GGAGGAGGAA GCGCTGACTT TAATGAGAAG CAGAGCTGGA AGAGCAGCCA GGGAACACCT 
    CAGAAACAGG CCGACATCAA CACACCACTG CAGTATAACG TCATGGAACA AAACAAACTC 
    AACAAGGACC ACTACCGAGG TGAGGGTCCC ATCTCAGTCA CCATCCCTTA TAACCAGGCC 
    CAGGACGTCC TCACAGGAGA AACCTACAGC AGCTCGGACA GAGGGAGCAG CACCTCTGGA 
    AGTCAAGGGC AGAAAAAGGG TGGACGAACA ACTAAACAAG CCAAACAGAA TCCAATGAAT 
    TGGACTGAAC TGTTGCCTCC TCCTCCGGTT AATCCGCCGC CATGTCAAGA ATACACCCTG 
    GTGATGGACG ACAGTTACGA CACAGACCTG CAGTGCCCTT TGCCGCCGTC TCGGATGTAT 
    CTGCAACCTG ACGAGTTGGA AGAGGAGGAG GACGAGGTGG AGCTGGAGCG TGGACCTACA 
    CCTCCTGTAC GGGGAGCTGC TTCTTCACCT GCTGCCGTGT CCTACAGCCA CCAGTCCACC 
    GCAACCCTAA CCCCGTCCCC TCAGGAGGAG ATGCATCCCA TGCTGCAGGA CAACCCTGAT 
    CTACATGAGC GCAGGAGACT GTTAGTGAGT CCACCTCCTC CACCACGACC TCTCTCTCCT 
    CCACACACAT ACGGCTACAT CTCCAGTCCC CTCGGCCTGG ACACGGACGG CCTAGAGGAA 
    GAGGATGATG ATGAAGAAGA GGGTGACGAA ACGGACGCAG AGGTAGCTCG AGTCCACCAG 
    CACCACATGA TGCACCCTCA CCAGCAGCAC TTGCCCCAGC AGAGGGCTCT CCTGCGGGGT 
    CTGGAACAAA CCCCGGCCTC CAGCACCTGT GACCTGGAGA GCTCTGTGAC TGGGTCTATG 
    ATTAACGGAT GGGGTTCGGC TTCAGAAGAG GACAACGCCT CGTCAGGCCG TTCCAGTGCA 
    GTCAGCTCAT CGGATGGATC GTTCTTTACC GATGCCGATT TCGCCCAGGC TGTGGCAGCT 
    GCGGCTGAAT ATGCTGGTCT TCGAGTGGCA AAGCATCCTG GGAAAGGACA TCATCAGCTT 
    CCAGTGTCTG GAGCACGGAA ATACCAAAAC CTCCCTGCGG GACACCGTCC AGGCAGCCCA 
    GTGTCCACAG ACAGTAACGT TAGCGCAGCC CCGGGTCAGA GGAGGCCCCT GAAGAGGCAA 
    AAACAACATC CAGCTGCTCA GACAGGCTAC CAGCGCCGAG AGGCCTACAC AGAGGATCTC 
    CCCCCTCCTC CGATTCCTCC TCCAGCCATG CTGAAGTCTC CAACACACCC GGCCAAAACC 
    ATCCCCGAGG GTCCACGGGG GGCCATGTCG CCAAGATCCA GCTCTGCGGA TGGCAAGGAC 
    AGGCGAACGG GTGCTGGCGG GCCCAGGGTC AGGGACGGTT CAGACACTCG GACCAGCTCT 
    TCAGAGCGGA GGGATGGCCA AGATGTACAG AGGGGTCAGA GAACGGGCAA GAATAGCAAA 
    CAAGATGAAG CCGTGAAGAG CCGCCAGCAC CCAGGCTCAG AGGACATCCT ACCTTACAGT 
    CGTCCTTCTT TCCCAGCCGT CCACAGTCCT CGAGGAGAAC GGGACCCCAG TTCCTCCAGT 
    TCACTGTCCT CTCGGGGGTC TGGCGGGCGA CGGAGAGGTG AAGGGCCACC AGGGGCCCGC 
    AGAAACCCTG CAGACATTGG GCTAAACACC ATAGGAGCCT TCCAGACTGG AGGGGACGAG 
    CTTGAGATGG TGGAGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001296753.1
    CDS699..5639
    Translation

    Target ORF information:

    RefSeq Version NM_001309824.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio roundabout, axon guidance receptor, homolog 1 (Drosophila) (robo1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001309824.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGGA GAATAATTGG ATTCGTGCTT TTTATCGCAG TTACACACGT TTGCACAGGA 
    TCCCGTCTGC GGCAGGAAGA TTACGCACCC CGTATTGTTG AACACCCCTC TGATCTGATC 
    GTATCCAAAG GCGAACCGGC CACCCTAAAC TGCAAGGCTG AAGGACGTCC CACGCCCACA 
    GTGGAGTGGT ACAAAGATGG AGAACGAGTT GAAACCGACC GGGACAATCC TCGATCCCAC 
    CGCATGCTCC TGCCCACCGG ATCTCTCTTC TTTCTCCGCA TTGTTCACGG CCGGCGCAGC 
    AAACCCGACG AAGGCTCCTA CGTTTGTGTG GCACGTAACT ATCTGGGAGA AGCCGTCAGC 
    CACAACGCCT CGCTGGAAGT AGCTATTCTC CGAGATGATT TCCGACAGAA CCCTGTGGAC 
    GTGATGGTGG CAGCGGGGGA GCCAGCTGTA ATGGAGTGCC AGCCGCCCCG AGGACACCCA 
    GAGCCCACCA TCTCCTGGAA GAAAGATGGG GTCAATATAG ACGACAGAGA CGAGAGGATA 
    ACGATACGAG GAGGAAAGCT CATGATCACC AACACCAGAA AAACGGACGC GGGGAAGTAC 
    GTGTGCGTGG GAACCAACAT GATGGGGGAA CGAGAGAGCG AGATCGCGGA GTTGACTGTT 
    CTCGAGAGGC CCAGTTTCTT GCGAAAGCCG AGCAGCCAGT CCGTCCTTGT GGATCAGAGC 
    GTTGAGTTCA GATGTGAAGC TCAAGGAGAC CCCGTGCCCA CCATCCGATG GAGGAAAGAG 
    GACGGAGAAC TGCCCAAGGG AAGGTATGAG ATCTGGGAGG ACCACACTCT CAAACTGAAG 
    AGGCTGACTT CAGGAGACGC CGGTACTTAC ACCTGTCAGG CGGAGAACAT GATGGGCAAG 
    ACGGAGGCCA GCGCCACCCT CACCGTCCAC GTTCCTCCAG TGTTTGTGGT CCGACCCAGG 
    AATCAGGTGG CGGGTATGGG CCGCACCGTC ACCTTTCAGT GTGAAGCCAC AGGAAGTCCT 
    CAGCCTGCAA TATTCTGGCA GAAAGAAGGC AGTCAGAATC TCCTGTTCTC GTCTCAGCCT 
    CCTCCTCCGT CCAGCAGATT CTCTGTGTCT CAGACTGGAG ATCTGACCAT CACAGCAGTT 
    GAGCGTTCAG ATGGAGGATA TTATAGCTGT CAGGCTCTCA ACATCGCTGG CAGTGTGATC 
    ACCAAAGCCC TGCTGGAGGT TACAGACGTG ATTTCAGACA GGCCTCCACC AATCGTCCGT 
    CAGGGTCCAA CCAACCAGAC TGTGGCAGTG GACGGGACGG TGGTTCTGAG CTGCCAAGCC 
    ACCGGGACAC CGATTCCTAC CATCTTATGG AGGAAAGATG GAGTTCTGGT GTCCACACAT 
    GATTCCCGAC TCAAGCAGCT GGACACTGGA GCCCTGCAGA TTCGATACGC AAAGCTGGGC 
    GACACTGGCA TGTACACCTG CACGGCTTCC ACCCCGAGCG GAGAGGCGTC CTGGAAGGCC 
    TACCTGGAGG TGCAAGAGTT TGGTGCTCCA GTTCAACCGG GCCGTCCCAC AGATCCCAAC 
    CTCATCCCCA GCGCCCCATC TAAACCAGAC GTCACGGATG TCAGCCGCAC CTCCGTGTCT 
    CTTTCCTGGA AGCCGAATCT GAACGCTGGA GCCACACCCA CCTCATACGT CATTGAGGCC 
    TTCAGCCATG CAGCAGGCAG CAGCTGGCAG ACGCTCGCCG ATCATGTGAA GACGGAAAGC 
    TTCGTGCTTA AGGGTCTGAA GCCAAGTGCC GTCTACCTGT TTCTGGTGAG AGCGGCCAAT 
    GCGTACGGCC TGAGCGACCC CAGTCCCATC AGCGACGCTG TCAAAACACA AGACGTCCCA 
    CCAACCAGCC AGGGAGTGGA GCATGGCCAG ATCCAGAGAG AACTGGGAGA TGTCGTCATT 
    CACCTTCACA ATCCCACAAT TCTCTCATCG TCTTCCGTCA GAGTCCAGTG GACTGTGGAG 
    CAGCAGTCTC AGTATATTCA GGGCTATAAG GTGATGTACC GTCCGTCTCC AGAAGGCGCT 
    CCGCAGCGAG TGGATTGGGC CATGTTTGAG GTCGGAGCCC CGGGGGAGCA CAGCGCTGTC 
    GTCACCCAGC TCAAGAAGGG CATCACCTAT GAGTTCAAAG TGCGCCCTTT CTTCAATGAG 
    TTCCAGGGCA CCGACAGCGA CATCAAAGTG GGAAAGACCC TGGAGGAGGC TCCCAGCGCT 
    GCTCCCAGAG GCGTCACCGT AACAGGAAGT GGAGACAACG GCACAGCAGT CCTGGTCGCC 
    TGGCAACCGC CGCCAGAGGA GGAGCAGAAC GGCGTTGTGC AGGAGTACAA GATCTGGTGT 
    CTTGGTAATG AAAGCCGATA CCACATCAAC CGCACAGTGG ATGGTTCTAC ACACTCGGTG 
    TTGATTCCTG GTCTGGTGGC AGGAGTCACA TACAGGCTAG AGGTGGCAGC AGGCACTGGG 
    GCCGGACCCG GGGTCAAGAG CGACCCTACG TCCTTCCAGC TTGACTCTTC TGGCCGTGTT 
    CTCCCGCCGC CGCTGGACGA CGATAACACC ATTTCCCAGC AGATCTCAGA CGTGGTGCGT 
    CAGCCGGCTT TCATCGCGGG CATCGGCGCC GCCTGCTGGA TCATCCTGAT GGTGTTCAGC 
    ATCTGGCTAT ACCGCCACCG CAAGAAGAGG AACGGCCTCA GCAGCAGCTA CGCCGGCATC 
    CGCAAAGTGC CCTCCTTCAC CTTCACCCCC ACAGTGGCCT ATCAGAGAGG CGGAGAGGCC 
    GTCAGCAGTG CAGGAAGGCC GGGTTTGTTG AATATAGGTG AACCAGCTAC ACAGCCCTGG 
    CTTGCGGACA CCTGGCCCAA CAGCTGCTCC AATCACAATG ACTGCACTAT TAACTGCTGC 
    ACCGGCAGCA ATGGAAACAG CGACAGCAAC CTGGCAACTT ACAGCCGCCC AGCCGACTGC 
    ATCGCCAACT ACAGCAACCA GATGGACAAC AAGGCCACCA ACCTGCTGGC TCCAGATACT 
    GGGCTTTACA GCGACGTTGA CCTTTCAAAC AAGATCAACG AGATGAAGAC CTTCAACAGC 
    CCCAACCTGA AGGACGGGCG TTTTATGGGG CCTGGTGGTC AACCCACACC CTACGCCACC 
    ACTCAGCTCA TTCAATCCAG CCTGGCCAAC AACAACATGA ACAGCAGCAG TGGCGGAAGC 
    GGAGGAGGAA GCGCTGACTT TAATGAGAAG CAGAGCTGGA AGAGCAGCCA GGGAACACCT 
    CAGAAACAGG CCGACATCAA CACACCACTG CAGTATAACG TCATGGAACA AAACAAACTC 
    AACAAGGACC ACTACCGAGG TGAGGGTCCC ATCTCAGTCA CCATCCCTTA TAACCAGGCC 
    CAGGACGTCC TCACAGGAGA AACCTACAGC AGCTCGGACA GAGGGAGCAG CACCTCTGGA 
    AGTCAAGGGC AGAAAAAGGG TGGACGAACA ACTAAACAAG CCAAACAGAA TCCAATGAAT 
    TGGACTGAAC TGTTGCCTCC TCCTCCGGTT AATCCGCCGC CATGTCAAGA ATACACCCTG 
    GTGATGGACG ACAGTTACGA CACAGACCTG CAGTGCCCTT TGCCGCCGTC TCGGATGTAT 
    CTGCAACCTG ACGAGTTGGA AGAGGAGGAG GACGAGGTGG AGCTGGAGCG TGGACCTACA 
    CCTCCTGTAC GGGGAGCTGC TTCTTCACCT GCTGCCGTGT CCTACAGCCA CCAGTCCACC 
    GCAACCCTAA CCCCGTCCCC TCAGGAGGAG ATGCATCCCA TGCTGCAGGA CAACCCTGAT 
    CTACATGAGC GCAGGAGACT GTTAGTGAGT CCACCTCCTC CACCACGACC TCTCTCTCCT 
    CCACACACAT ACGGCTACAT CTCCAGTCCC CTCGGCCTGG ACACGGACGG CCTAGAGGAA 
    GAGGATGATG ATGAAGAAGA GGGTGACGAA ACGGACGCAG AGGTAGCTCG AGTCCACCAG 
    CACCACATGA TGCACCCTCA CCAGCAGCAC TTGCCCCAGC AGAGGGCTCT CCTGCGGGGT 
    CTGGAACAAA CCCCGGCCTC CAGCACCTGT GACCTGGAGA GCTCTGTGAC TGGGTCTATG 
    ATTAACGGAT GGGGTTCGGC TTCAGAAGAG GACAACGCCT CGTCAGGCCG TTCCAGTGCA 
    GTCAGCTCAT CGGATGGATC GTTCTTTACC GATGCCGATT TCGCCCAGGC TGTGGCAGCT 
    GCGGCTGAAT ATGCTGGTCT TCGAGTGGCA AAGCATCCTG GGAAAGGACA TCATCAGCTT 
    CCAGTGTCTG GAGCACGGAA ATACCAAAAC CTCCCTGCGG GACACCGTCC AGGCAGCCCA 
    GTGTCCACAG ACAGTAACGT TAGCGCAGCC CCGGGTCAGA GGAGGCCCCT GAAGAGGCAA 
    AAACAACATC CAGCTGCTCA GACAGGCTAC CAGCGCCGAG AGGCCTACAC AGAGGATCTC 
    CCCCCTCCTC CGATTCCTCC TCCAGCCATG CTGAAGTCTC CAACACACCC GGCCAAAACC 
    ATCCCCGAGG GTCCACGGGG GGCCATGTCG CCAAGATCCA GCTCTGCGGA TGGCAAGGAC 
    AGGCGAACGG GTGCTGGCGG GCCCAGGGTC AGGGACGGTT CAGACACTCG GACCAGCTCT 
    TCAGAGCGGA GGGATGGCCA AGATGTACAG AGGGGTCAGA GAACGGGCAA GAATAGCAAA 
    CAAGATGAAG CCGTGAAGAG CCGCCAGCAC CCAGGCTCAG AGGACATCCT ACCTTACAGT 
    CGTCCTTCTT TCCCAGCCGT CCACAGTCCT CGAGGAGAAC GGGACCCCAG TTCCTCCAGT 
    TCACTGTCCT CTCGGGGGTC TGGCGGGCGA CGGAGAGGTG AAGGGCCACC AGGGGCCCGC 
    AGAAACCCTG CAGACATTGG GCTAAACACC ATAGGAGCCT TCCAGACTGG AGGGGACGAG 
    CTTGAGATGG TGGAGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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