sphkap cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sphkap
Entrez Gene ID	100034387
Full Name	SPHK1 interactor, AKAP domain containing
Synonyms	si:dkey-223n17.6,si:dkey-99o15.1
General protein information	Preferred Names SPHK1 interactor, AKAP domain containing Names A-kinase anchor protein SPHKAP SPHK1-interactor and AKAP domain-containing protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009293856.3	XP_009292131.1	A-kinase anchor protein SPHKAP isoform X1
XM_009293859.3	XP_009292134.1	A-kinase anchor protein SPHKAP isoform X3
XM_009293856.2	XP_009292131.1	A-kinase anchor protein SPHKAP isoform X1
XM_009293857.2	XP_009292132.1	A-kinase anchor protein SPHKAP isoform X2
XM_009293859.2	XP_009292134.1	A-kinase anchor protein SPHKAP isoform X3
NM_001083045.1	NP_001076514.1	A-kinase anchor protein SPHKAP
XM_009293858.2	XP_009292133.1	A-kinase anchor protein SPHKAP isoform X2
XM_009293857.3	XP_009292132.1	A-kinase anchor protein SPHKAP isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPHKAP	XP_005635852.1
Gallus gallus (chicken)	SPHKAP	NP_001171003.1
Xenopus tropicalis (tropical clawed frog)	sphkap	XP_002933413.2
Bos taurus (cattle)	SPHKAP	XP_002685689.2
Mus musculus (house mouse)	Sphkap	NP_766018.3
Danio rerio (zebrafish)	sphkap	NP_001076514.1
Homo sapiens (human)	SPHKAP	NP_001136116.1
Pan troglodytes (chimpanzee)	SPHKAP	XP_001137841.1
	SPHKAP	XP_001111587.1
Rattus norvegicus (Norway rat)	Sphkap	NP_001120964.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Dner inhibits neural progenitor proliferation and induces neuronal and glial differentiation in zebrafish.
Hsieh FY, Ma TL, Shih HY, Lin SJ, Huang CW, Wang HY, Cheng YC
Developmental biology375(1)1-12(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sphkap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sphkap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00240	NM_001083045.1 Latest version!	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa48351	XM_009293856.3 Latest version!	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa48352	XM_009293858.2 Latest version!	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa48352	XM_009293857.3 Latest version!	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa48353	XM_009293859.3 Latest version!	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa48351	XM_009293856.2	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa48353	XM_009293859.2	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa48352	XM_009293857.2	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sphkap ( NM_001083045.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001076514.1 Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), mRNA.

Price & Availability↑

CloneID	ODa00240
Clone ID Related Accession (Same CDS sequence)	NM_001083045.1
Accession Version	NM_001083045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein SPHKAP
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX649602.5 and BX908751.8. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA4476790 [ECO:0000350] ##Evidence-Data-END##

ATGCCGCTCC TCTTCTTCGC GCTCGCCGGC TGTGACGGCT CACCTCTGTG TGGAGGGCTT 
TCCCAGCGCA ACCAGTTTGT CCGCTTAGCT TGTAGTGTTA CTAATTTCCA ATCATCTGCC 
ATGTTTGAGG GATCTGAGTC CGCGAACACA CAGGAAGTCA AATCTGAGAG CACCCTTGGG 
TCCTCGCTCT CCGCCTGCAA GAAGGTTTTG TGCAGTAACA GTGTTCTGGA TTCGTCTGAG 
TACTGGCTGA AGAATGATAA GACGCTCTGC AGAATTGGAT TTCTGGAGGA CCAGCATGAC 
AGCGGCTGCC CGACAATTTG CTTTGTAAAC CTGGACAGAC ATACTTCAGA TTGGCATGAT 
GACAACTACA TCAAGAAGCT GGCCTCGGTG TGCCCAGAGC TGCCACGGTT AATTGAGTCT 
CTCGGAGTGC GGCAACCCAA AGAAAATGAG ATTCTGCTTC TCAGCAGCCT AGAGCCACCG 
GACTCCTGCC AGCATGACCC CAGCCACCCA CAGAGCCCGA GAACAGCGGA TGTCTGTCTG 
GTGCATTGTT CATGTGGACG CCGTCCCACC CAAACGAGCA GTATCATTTT TGAGATTAAC 
AAGTTCCTGA TTGGACTACA GTCAGGGCAG GAGAGGCAGA AGCATGGCCG ATTGGCTGGA 
CAACGAGCTG CTGAAGATGA CACCAATCGC TCTGTTTCGT CGATTGAAGA AGATTTCCTC 
ACTGCCTCAG AGCAGCTTGG GGAAGACAGC GAGGAAGACC CTTTCAGGAA TGATCCTGAG 
AATTCTCTCA TGCCCGAGTC TGTTAAGGTG TTGAGAGCAG CCCACGCTCA AAAAAGGAGT 
GAAATAGAGA GAGAGGACAG TGAGGACTCT GAAACCCTCT GCACCTCTTC CAACTCCCAA 
AAGCTTTCAA GAACATATTC CGCCCCTGCA AGAGGCCGAC CCACCACCCC AAAACAAGTC 
AAGGAGTCAG CCGGTCACTA CGCCACCAAT CTGGCTGAGT CAGTCCTGCA AGATGCTTTT 
ATTCGTTTGT CACAAGATGA GCCATCATTT GTTGCAGAAG CAGCAGTGAG TGTGTCAAGT 
GATATAAGCC ATTCCACTGA GGCACCACAG AGGGCTCGAG CTTGCTCATT CGAACTTCCT 
AAAATTGTGA TTGTCCAAAG TCCTGATAAC AGTGAGGAGG TAACAGAATG GCCGGAAGTG 
CAGTCACATG CAACATCAGA GCATGATAGT GGAAATAAGG CCAAAGCAAA GCATGGTACT 
CATCCCCCGC ACAACTCGCC AGTTGGACAC CCTGCTAAGC CATTGGAAAT AGCTTTGGCA 
TGTGCTGCAA GTGTCATTGG TACCATTACC ACCCCTCAAG TCACAGAACA ACTCACACTA 
GAGTCAGGAG AGGAATATGA GTGTGAAGAT GAAGAGGAAG AAAGTGAAAC TGATCAAGGG 
GAGTATTCGT TTTCATCTGC GGTATGTGGC ATGTCTCAGG TCGCTGGAGC TGTAGCTGCG 
GTCGATCTAG CAGATGACTC TGGGGACAAT GAAGACTTGG CAGACATGTA TTCCGCCTCT 
ATGGGACTTC TGTCAGTTGC CCAAGCTTCA ACTGCAATAC CTCTTCACTG TAGCATAGCA 
GAGGGCACCA GCGTTGAGGC CTTTCGAGCC AATGTGGCTG AAGTTCTCCT CAGGGAAGCT 
TCAGCTGTAT TCACTCACAG ACAAAGTTAT TCAAGTGTTG CAAATTTCCT TGAGACCACA 
CATAACAAGA TAGTGGATGG CATCACAAAT CCAAGAAGAC CGTATCAGGA GCAACAAGAT 
GTGGATAACT TCACTCAAGA AATATCTGAC AGTATCTTTC AGTATGCCCT TGAGAAGGCG 
GAGAAGAGAA AAGAACTTGA GGGTCCTGGA AAAGATGCCC CTAACATTGA GGGTTTCCTC 
TCGGATTGTG TGAATAATTT GCTCTTTGAT GTACTGTGTG TAACATCAAG GAAAATAAGT 
GACATTTCAA ATTGTAATAT GGAATCATGT GATGGGCAGG AAGGCAGTGT CGGCTATAGG 
GCATATGAGG CTGATTCCAA AGCTGGCAGG GAAGCATTGA GCCAATTGCA GCATTTGATT 
GAGCCAAGCC AGGCTTATAA TCATGGTGAA AGGCGAAGCA GTGTGGAGAA GTTATCTGCT 
CTAATAGGAA AGAGAGAGAG AGAGCAGAAA CACATACAGG AGGAAAGAGA AAATGAAGCC 
AGACTGAAGG AACCCTACAG ACTTGCTCTG AACAGAGTGA CAGCCACTAT GGTCAAAGAG 
CAGTCTGACC TGCAAGGGCA GCTGGGCAGG AGTGACAAGA ATGCAGACTC TGCGCTCCCT 
TCAGCAGAAA GCTCACCACT TCACATGCAA CGAGGGAGGG CAGGAGGAGA GAGCGAGACC 
AGGCAGCAGT CTTTATCGTC CTCTTCTGGA GCACTTGTGG CACTTAAAAT GGATTCGGGG 
GAGTCCAAAA CTCCTGTCAC GTGCTTTGCT GAAGATTTGG CGACCACAGT GGTCTCCATG 
GCTACAGAGC TGGCAGCGAT ATGCTTGGAG AACTCAAGTG GAAAGCAGCC GTGGTTCTGC 
GCATTAAATG GTGGCTCGGA AGGTCCAGAA GGGCTGCTTC TGCCCTGCCG CACAGCTGCA 
GCTCTCCGCC GAAAGGAGAC ACAAAATGGC ACCTCGGTCA CCAAGAAGCA CAGACCGCCA 
CGTCTCAGTG AAATCAAACG TAAAACCGAA GAACAGCCCG AACTCATGGA GCGCCTGGTC 
AACCGTGTTG TAGATGAAAC CGTTAACTTA GATGAACCCA CAACCACTGA TCCATTCGCG 
CTCTTTGCAT CAGAGGTCAC TGCCAGGATA ATGAACTGCC CAGAATTAAG TGTAGTGGAC 
ACTTCCAAAT CTGGGCAGTC GTCCCGCAGC CGCCTGCAGT GTGAGAGATG GAGTAGCAGG 
GGGAAGGCAT CAAGCTATGA GAGCATTCCA GAGGAGGATG CAGATCCCTC CGGGACATCA 
AATACTTTGG GTCCAGGAAA CAGGCTTGGG CATAATTTGA GCCGAGGAAG CTCTATCTCC 
AAGCAGTCCA GCTGTGAGAG CATTACGGAT GAGTTCTCTC GCTTCATGGT GAACCAGATG 
GAGACAGAGG GTCGAGGGTT TGACCTTCTG CTGGACTACT ATGCTGGGAA AAATGCCAGC 
AGCATCCTTG CAGCAGCTGT TCAGCAGGCT GCCAGTAAAA AAAACGGACA CCTCAATGTG 
CGTACATCAT CTTGTCTCTC GAAGCAGTCC AGCACTGAGA GTATCACAGA AGAGTTTTAT 
CGCTTCATGC TGAAAGATAT GGACAAGGAA AACAAGGACT ACAGTATGAC CAAGACCAAA 
GAGTGGAGTA ATAGCCTGCT ACCCCCGTCC CCCAGAACCC CTTTTTGTAT CCGTCAGTCT 
TCTGTTCCTG ACAGGCGGTC TTCAGATTCC AGACTAACTG TAAACTCGCC TATCAAGGCC 
AATTCATTCG ATGGCTTTGC TCAAAATGTA CACGGGGACT CGCTTAACAT CTATCCAACT 
AACTCTGTGT CGTCATCTGG ACTCTGCAAG TCCGATTCCT GCTTGTATAA ACGCGGCCAA 
ACAGACCAAA TCACAGACAT GCTGATTCAT GAAACCTGGT CCAGCTCTAT CGAATCTCTA 
ATGCGCAAGA ATAAGATAAT TGCCGAGCCC TCAGAGGACA GTCTGGAACT CGATTCTGCA 
GACTCACAGC CACATGTACA GCAGTTTGCT AATCGTCTAG CGGCAGACAT CGTAGAGAGT 
GGCAAATCAC AGCTTGGAGG CCAGCAGGAT GTCACTGCGG CTGCTTGTCA GCCACATATT 
CCAGTTGGAG AAAGGAGACA CGGCTTTAAA CATTCCCGTT GCAACAACAG TGGAAACCGG 
CCAGGCGTGG AGCATCAGGA AAACAGCTGT AGCTCTTACA GTTCCTCCTG TGTGAGGCAG 
GCATGGCGCG GACAGAGAGA GGTACCCTTG ATTCACATTG AACCAGATCA AAGAGAAGAG 
GCCTCGGAAG AGAAGGGAGG GGTTGAGACC CACCACAGGG AGGCATCCCA TCAGACCCAG 
CAACAAAGTG GCAAAGGGAG TGAGACAGCA ACCAAAAGCA GCAGTGACTC AGACAGGGTC 
TCCTCAGTGG CCACAGTGCC CCCTGCTGGT GTGGAGGTGG AGCGGCGGTC TCTCAGCGCT 
AGCAGTGAGG AAAGTGGCTC AGGCAGCTGG GCACAGATTG CCACTGAAGA CGACCCCCAG 
GAGGAGACCA CCAGTAGCTT TATCCAGCTA AGCGAGGGAA ATGGGAACAG CAGCACTTCT 
AGTCTGGGAC TGGCCGACCT AGAGGGCTTT CCGGACTTCT CCATCTCCAG CAACCTAATC 
AGTGAGGAGA GGGAGAGGAA GACTTCGCAC TGTCAGGACC AGGCGGACGT AACACTGGTT 
GTGTTGTCAG AGGTGACGTC GGGCTTGTCG ACGGCCGGCA GCAGCTGTCA GCGGGAGCTC 
CTGGTGCTGA ACTGTGACCT GGAGCCGGAC TGTGTGGACG GAGAGCTGAG AGCCGCGCTG 
CAGTGGATCT CAGCATCTGA ACTCGGCGTC CCGGCGCTTT ACTTCAGAAA GACTCACCAG 
CACAATCTTA CAAAGCTCCA CAGAGTGCTG CAGTTGGCAG GTCAGAAGGC TTGGCGTGTT 
GGAGACTTGT TCAGCGCCGT GGCTCAGTTC TGTCAGCTCC ACCAGGACCT GGAGCTGAAA 
GGACGGCCGC TGCCCAGCCT GTTTGACTGG ATCCTGAAGA CCAAACGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076514.1
CDS	1..4791
Translation	MPLLFFALAGCDGSPLCGGLSQRNQFVRLACSVTNFQSSAMFEGSESANTQEVKSESTLGSSLSACKKVLCSNSVLDSSEYWLKNDKTLCRIGFLEDQHDSGCPTICFVNLDRHTSDWHDDNYIKKLASVCPELPRLIESLGVRQPKENEILLLSSLEPPDSCQHDPSHPQSPRTADVCLVHCSCGRRPTQTSSIIFEINKFLIGLQSGQERQKHGRLAGQRAAEDDTNRSVSSIEEDFLTASEQLGEDSEEDPFRNDPENSLMPESVKVLRAAHAQKRSEIEREDSEDSETLCTSSNSQKLSRTYSAPARGRPTTPKQVKESAGHYATNLAESVLQDAFIRLSQDEPSFVAEAAVSVSSDISHSTEAPQRARACSFELPKIVIVQSPDNSEEVTEWPEVQSHATSEHDSGNKAKAKHGTHPPHNSPVGHPAKPLEIALACAASVIGTITTPQVTEQLTLESGEEYECEDEEEESETDQGEYSFSSAVCGMSQVAGAVAAVDLADDSGDNEDLADMYSASMGLLSVAQASTAIPLHCSIAEGTSVEAFRANVAEVLLREASAVFTHRQSYSSVANFLETTHNKIVDGITNPRRPYQEQQDVDNFTQEISDSIFQYALEKAEKRKELEGPGKDAPNIEGFLSDCVNNLLFDVLCVTSRKISDISNCNMESCDGQEGSVGYRAYEADSKAGREALSQLQHLIEPSQAYNHGERRSSVEKLSALIGKREREQKHIQEERENEARLKEPYRLALNRVTATMVKEQSDLQGQLGRSDKNADSALPSAESSPLHMQRGRAGGESETRQQSLSSSSGALVALKMDSGESKTPVTCFAEDLATTVVSMATELAAICLENSSGKQPWFCALNGGSEGPEGLLLPCRTAAALRRKETQNGTSVTKKHRPPRLSEIKRKTEEQPELMERLVNRVVDETVNLDEPTTTDPFALFASEVTARIMNCPELSVVDTSKSGQSSRSRLQCERWSSRGKASSYESIPEEDADPSGTSNTLGPGNRLGHNLSRGSSISKQSSCESITDEFSRFMVNQMETEGRGFDLLLDYYAGKNASSILAAAVQQAASKKNGHLNVRTSSCLSKQSSTESITEEFYRFMLKDMDKENKDYSMTKTKEWSNSLLPPSPRTPFCIRQSSVPDRRSSDSRLTVNSPIKANSFDGFAQNVHGDSLNIYPTNSVSSSGLCKSDSCLYKRGQTDQITDMLIHETWSSSIESLMRKNKIIAEPSEDSLELDSADSQPHVQQFANRLAADIVESGKSQLGGQQDVTAAACQPHIPVGERRHGFKHSRCNNSGNRPGVEHQENSCSSYSSSCVRQAWRGQREVPLIHIEPDQREEASEEKGGVETHHREASHQTQQQSGKGSETATKSSSDSDRVSSVATVPPAGVEVERRSLSASSEESGSGSWAQIATEDDPQEETTSSFIQLSEGNGNSSTSSLGLADLEGFPDFSISSNLISEERERKTSHCQDQADVTLVVLSEVTSGLSTAGSSCQRELLVLNCDLEPDCVDGELRAALQWISASELGVPALYFRKTHQHNLTKLHRVLQLAGQKAWRVGDLFSAVAQFCQLHQDLELKGRPLPSLFDWILKTKR

Target ORF information:

RefSeq Version	NM_001083045.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083045.1

ORF Insert Sequence:

ATGCCGCTCC TCTTCTTCGC GCTCGCCGGC TGTGACGGCT CACCTCTGTG TGGAGGGCTT 
TCCCAGCGCA ACCAGTTTGT CCGCTTAGCT TGTAGTGTTA CTAATTTCCA ATCATCTGCC 
ATGTTTGAGG GATCTGAGTC CGCGAACACA CAGGAAGTCA AATCTGAGAG CACCCTTGGG 
TCCTCGCTCT CCGCCTGCAA GAAGGTTTTG TGCAGTAACA GTGTTCTGGA TTCGTCTGAG 
TACTGGCTGA AGAATGATAA GACGCTCTGC AGAATTGGAT TTCTGGAGGA CCAGCATGAC 
AGCGGCTGCC CGACAATTTG CTTTGTAAAC CTGGACAGAC ATACTTCAGA TTGGCATGAT 
GACAACTACA TCAAGAAGCT GGCCTCGGTG TGCCCAGAGC TGCCACGGTT AATTGAGTCT 
CTCGGAGTGC GGCAACCCAA AGAAAATGAG ATTCTGCTTC TCAGCAGCCT AGAGCCACCG 
GACTCCTGCC AGCATGACCC CAGCCACCCA CAGAGCCCGA GAACAGCGGA TGTCTGTCTG 
GTGCATTGTT CATGTGGACG CCGTCCCACC CAAACGAGCA GTATCATTTT TGAGATTAAC 
AAGTTCCTGA TTGGACTACA GTCAGGGCAG GAGAGGCAGA AGCATGGCCG ATTGGCTGGA 
CAACGAGCTG CTGAAGATGA CACCAATCGC TCTGTTTCGT CGATTGAAGA AGATTTCCTC 
ACTGCCTCAG AGCAGCTTGG GGAAGACAGC GAGGAAGACC CTTTCAGGAA TGATCCTGAG 
AATTCTCTCA TGCCCGAGTC TGTTAAGGTG TTGAGAGCAG CCCACGCTCA AAAAAGGAGT 
GAAATAGAGA GAGAGGACAG TGAGGACTCT GAAACCCTCT GCACCTCTTC CAACTCCCAA 
AAGCTTTCAA GAACATATTC CGCCCCTGCA AGAGGCCGAC CCACCACCCC AAAACAAGTC 
AAGGAGTCAG CCGGTCACTA CGCCACCAAT CTGGCTGAGT CAGTCCTGCA AGATGCTTTT 
ATTCGTTTGT CACAAGATGA GCCATCATTT GTTGCAGAAG CAGCAGTGAG TGTGTCAAGT 
GATATAAGCC ATTCCACTGA GGCACCACAG AGGGCTCGAG CTTGCTCATT CGAACTTCCT 
AAAATTGTGA TTGTCCAAAG TCCTGATAAC AGTGAGGAGG TAACAGAATG GCCGGAAGTG 
CAGTCACATG CAACATCAGA GCATGATAGT GGAAATAAGG CCAAAGCAAA GCATGGTACT 
CATCCCCCGC ACAACTCGCC AGTTGGACAC CCTGCTAAGC CATTGGAAAT AGCTTTGGCA 
TGTGCTGCAA GTGTCATTGG TACCATTACC ACCCCTCAAG TCACAGAACA ACTCACACTA 
GAGTCAGGAG AGGAATATGA GTGTGAAGAT GAAGAGGAAG AAAGTGAAAC TGATCAAGGG 
GAGTATTCGT TTTCATCTGC GGTATGTGGC ATGTCTCAGG TCGCTGGAGC TGTAGCTGCG 
GTCGATCTAG CAGATGACTC TGGGGACAAT GAAGACTTGG CAGACATGTA TTCCGCCTCT 
ATGGGACTTC TGTCAGTTGC CCAAGCTTCA ACTGCAATAC CTCTTCACTG TAGCATAGCA 
GAGGGCACCA GCGTTGAGGC CTTTCGAGCC AATGTGGCTG AAGTTCTCCT CAGGGAAGCT 
TCAGCTGTAT TCACTCACAG ACAAAGTTAT TCAAGTGTTG CAAATTTCCT TGAGACCACA 
CATAACAAGA TAGTGGATGG CATCACAAAT CCAAGAAGAC CGTATCAGGA GCAACAAGAT 
GTGGATAACT TCACTCAAGA AATATCTGAC AGTATCTTTC AGTATGCCCT TGAGAAGGCG 
GAGAAGAGAA AAGAACTTGA GGGTCCTGGA AAAGATGCCC CTAACATTGA GGGTTTCCTC 
TCGGATTGTG TGAATAATTT GCTCTTTGAT GTACTGTGTG TAACATCAAG GAAAATAAGT 
GACATTTCAA ATTGTAATAT GGAATCATGT GATGGGCAGG AAGGCAGTGT CGGCTATAGG 
GCATATGAGG CTGATTCCAA AGCTGGCAGG GAAGCATTGA GCCAATTGCA GCATTTGATT 
GAGCCAAGCC AGGCTTATAA TCATGGTGAA AGGCGAAGCA GTGTGGAGAA GTTATCTGCT 
CTAATAGGAA AGAGAGAGAG AGAGCAGAAA CACATACAGG AGGAAAGAGA AAATGAAGCC 
AGACTGAAGG AACCCTACAG ACTTGCTCTG AACAGAGTGA CAGCCACTAT GGTCAAAGAG 
CAGTCTGACC TGCAAGGGCA GCTGGGCAGG AGTGACAAGA ATGCAGACTC TGCGCTCCCT 
TCAGCAGAAA GCTCACCACT TCACATGCAA CGAGGGAGGG CAGGAGGAGA GAGCGAGACC 
AGGCAGCAGT CTTTATCGTC CTCTTCTGGA GCACTTGTGG CACTTAAAAT GGATTCGGGG 
GAGTCCAAAA CTCCTGTCAC GTGCTTTGCT GAAGATTTGG CGACCACAGT GGTCTCCATG 
GCTACAGAGC TGGCAGCGAT ATGCTTGGAG AACTCAAGTG GAAAGCAGCC GTGGTTCTGC 
GCATTAAATG GTGGCTCGGA AGGTCCAGAA GGGCTGCTTC TGCCCTGCCG CACAGCTGCA 
GCTCTCCGCC GAAAGGAGAC ACAAAATGGC ACCTCGGTCA CCAAGAAGCA CAGACCGCCA 
CGTCTCAGTG AAATCAAACG TAAAACCGAA GAACAGCCCG AACTCATGGA GCGCCTGGTC 
AACCGTGTTG TAGATGAAAC CGTTAACTTA GATGAACCCA CAACCACTGA TCCATTCGCG 
CTCTTTGCAT CAGAGGTCAC TGCCAGGATA ATGAACTGCC CAGAATTAAG TGTAGTGGAC 
ACTTCCAAAT CTGGGCAGTC GTCCCGCAGC CGCCTGCAGT GTGAGAGATG GAGTAGCAGG 
GGGAAGGCAT CAAGCTATGA GAGCATTCCA GAGGAGGATG CAGATCCCTC CGGGACATCA 
AATACTTTGG GTCCAGGAAA CAGGCTTGGG CATAATTTGA GCCGAGGAAG CTCTATCTCC 
AAGCAGTCCA GCTGTGAGAG CATTACGGAT GAGTTCTCTC GCTTCATGGT GAACCAGATG 
GAGACAGAGG GTCGAGGGTT TGACCTTCTG CTGGACTACT ATGCTGGGAA AAATGCCAGC 
AGCATCCTTG CAGCAGCTGT TCAGCAGGCT GCCAGTAAAA AAAACGGACA CCTCAATGTG 
CGTACATCAT CTTGTCTCTC GAAGCAGTCC AGCACTGAGA GTATCACAGA AGAGTTTTAT 
CGCTTCATGC TGAAAGATAT GGACAAGGAA AACAAGGACT ACAGTATGAC CAAGACCAAA 
GAGTGGAGTA ATAGCCTGCT ACCCCCGTCC CCCAGAACCC CTTTTTGTAT CCGTCAGTCT 
TCTGTTCCTG ACAGGCGGTC TTCAGATTCC AGACTAACTG TAAACTCGCC TATCAAGGCC 
AATTCATTCG ATGGCTTTGC TCAAAATGTA CACGGGGACT CGCTTAACAT CTATCCAACT 
AACTCTGTGT CGTCATCTGG ACTCTGCAAG TCCGATTCCT GCTTGTATAA ACGCGGCCAA 
ACAGACCAAA TCACAGACAT GCTGATTCAT GAAACCTGGT CCAGCTCTAT CGAATCTCTA 
ATGCGCAAGA ATAAGATAAT TGCCGAGCCC TCAGAGGACA GTCTGGAACT CGATTCTGCA 
GACTCACAGC CACATGTACA GCAGTTTGCT AATCGTCTAG CGGCAGACAT CGTAGAGAGT 
GGCAAATCAC AGCTTGGAGG CCAGCAGGAT GTCACTGCGG CTGCTTGTCA GCCACATATT 
CCAGTTGGAG AAAGGAGACA CGGCTTTAAA CATTCCCGTT GCAACAACAG TGGAAACCGG 
CCAGGCGTGG AGCATCAGGA AAACAGCTGT AGCTCTTACA GTTCCTCCTG TGTGAGGCAG 
GCATGGCGCG GACAGAGAGA GGTACCCTTG ATTCACATTG AACCAGATCA AAGAGAAGAG 
GCCTCGGAAG AGAAGGGAGG GGTTGAGACC CACCACAGGG AGGCATCCCA TCAGACCCAG 
CAACAAAGTG GCAAAGGGAG TGAGACAGCA ACCAAAAGCA GCAGTGACTC AGACAGGGTC 
TCCTCAGTGG CCACAGTGCC CCCTGCTGGT GTGGAGGTGG AGCGGCGGTC TCTCAGCGCT 
AGCAGTGAGG AAAGTGGCTC AGGCAGCTGG GCACAGATTG CCACTGAAGA CGACCCCCAG 
GAGGAGACCA CCAGTAGCTT TATCCAGCTA AGCGAGGGAA ATGGGAACAG CAGCACTTCT 
AGTCTGGGAC TGGCCGACCT AGAGGGCTTT CCGGACTTCT CCATCTCCAG CAACCTAATC 
AGTGAGGAGA GGGAGAGGAA GACTTCGCAC TGTCAGGACC AGGCGGACGT AACACTGGTT 
GTGTTGTCAG AGGTGACGTC GGGCTTGTCG ACGGCCGGCA GCAGCTGTCA GCGGGAGCTC 
CTGGTGCTGA ACTGTGACCT GGAGCCGGAC TGTGTGGACG GAGAGCTGAG AGCCGCGCTG 
CAGTGGATCT CAGCATCTGA ACTCGGCGTC CCGGCGCTTT ACTTCAGAAA GACTCACCAG 
CACAATCTTA CAAAGCTCCA CAGAGTGCTG CAGTTGGCAG GTCAGAAGGC TTGGCGTGTT 
GGAGACTTGT TCAGCGCCGT GGCTCAGTTC TGTCAGCTCC ACCAGGACCT GGAGCTGAAA 
GGACGGCCGC TGCCCAGCCT GTTTGACTGG ATCCTGAAGA CCAAACGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sphkap ( XM_009293856.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292131.1 Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48351
Clone ID Related Accession (Same CDS sequence)	XM_009293856.3 , XM_009293856.2
Accession Version	XM_009293856.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293856.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGCAA ACCTGCTCAG CGTCCTCCAA AACTTCACAG AGAGTAATTT CCAATCATCT 
GCCATGTTTG AGGGATCTGA GTCCGCGAAC ACACAGGAAG TCAAATCTGA GAGCACCCTT 
GGGTCCTCGC TCTCCGCCTG CAAGAAGGTT TTGTGCAGTA ACAGTGTTCT GGATTCGTCT 
GAGTACTGGC TGAAGAATGA TAAGACGCTC TGCAGAATTG GATTTCTGGA GGACCAGCAT 
GACAGCGGCT GCCCGACAAT TTGCTTTGTA AACCTGGACA GACATACTTC AGATTGGCAT 
GATGACAACT ACATCAAGAA GCTGGCCTCG GTGTGCCCAG AGCTGCCACG GTTAATTGAG 
TCTCTCGGAG TGCGGCAACC CAAAGAAAAT GAGATTCTGC TTCTCAGCAG CCTAGAGCCA 
CCGGACTCCT GCCAGCATGA CCCCAGCCAC CCACAGAGCC CGAGAACAGC GGATGTCTGT 
CTGGTGCATT GTTCATGTGG ACGCCGTCCC ACCCAAACGA GCAGTATCAT TTTTGAGATT 
AACAAGTTCC TGATTGGACT ACAGTCAGGG CAGGAGAGGC AGAAGCATGG CCGATTGGCT 
GGACAACGAG CTGCTGAAGA TGACACCAAT CGCTCTGTTT CGTCGATTGA AGAAGATTTC 
CTCACTGCCT CAGAGCAGCT TGGGGAAGAC AGCGAGGAAG ACCCTTTCAG GAATGATCCT 
GAGAATTCTC TCATGCCCGA GTCTGTTAAG GTGTTGAGAG CAGCCCACGC TCAAAAAAGG 
AGTGAAATAG AGAGAGAGGA CAGTGAGGAC TCTGAAACCC TCTGCACCTC TTCCAACTCC 
CAAAAGCTTT CAAGAACATA TTCCGCCCCT GCAAGAGGCC GACCCACCAC CCCAAAACAA 
GTCAAGGAGT CAGCCGGTCA CTACGCCACC AATCTGGCTG AGTCAGTCCT GCAAGATGCT 
TTTATTCGTT TGTCACAAGA TGAGCCATCA TTTGTTGCAG AAGCAGCAGT GAGTGTGTCA 
AGTGATATAA GCCATTCCAC TGAGGCACCA CAGAGGGCTC GAGCTTGCTC ATTCGAACTT 
CCTAAAATTG TGATTGTCCA AAGTCCTGAT AACAGTGAGG AGGTAACAGA ATGGCCGGAA 
GTGCAGTCAC ATGCAACATC AGAGCATGAT AGTGGAAATA AGGCCAAAGC AAAGCATGGT 
ACTCATCCCC CGCACAACTC GCCAGTTGGA CACCCTGCTA AGCCATTGGA AATAGCTTTG 
GCATGTGCTG CAAGTGTCAT TGGTACCATT ACCACCCCTC AAGTCACAGA ACAACTCACA 
CTAGAGTCAG GAGAGGAATA TGAGTGTGAA GATGAAGAGG AAGAAAGTGA AACTGATCAA 
GGGGAGTATT CGTTTTCATC TGCGGTATGT GGCATGTCTC AGGTCGCTGG AGCTGTAGCT 
GCGGTCGATC TAGCAGATGA CTCTGGGGAC AATGAAGACT TGGCAGACAT GTATTCCGCC 
TCTATGGGAC TTCTGTCAGT TGCCCAAGCT TCAACTGCAA TACCTCTTCA CTGTAGCATA 
GCAGAGGGCA CCAGCGTTGA GGCCTTTCGA GCCAATGTGG CTGAAGTTCT CCTCAGGGAA 
GCTTCAGCTG TATTCACTCA CAGACAAAGT TATTCAAGTG TTGCAAATTT CCTTGAGACC 
ACACATAACA AGATAGTGGA TGGCATCACA AATCCAAGAA GACCGTATCA GGAGCAACAA 
GATGTGGATA ACTTCACTCA AGAAATATCT GACAGTATCT TTCAGTATGC CCTTGAGAAG 
GCGGAGAAGA GAAAAGAACT TGAGGGTCCT GGAAAAGATG CCCCTAACAT TGAGGGTTTC 
CTCTCGGATT GTGTGAATAA TTTGCTCTTT GATGTACTGT GTGTAACATC AAGGAAAATA 
AGTGACATTT CAAATTGTAA TATGGAATCA TGTGATGGGC AGGAAGGCAG TGTCGGCTAT 
AGGGCATATG AGGCTGATTC CAAAGCTGGC AGGGAAGCAT TGAGCCAATT GCAGCATTTG 
ATTGAGCCAA GCCAGGCTTA TAATCATGGT GAAAGGCGAA GCAGTGTGGA GAAGTTATCT 
GCTCTAATAG GAAAGAGAGA GAGAGAGCAG AAACACATAC AGGAGGAAAG AGAAAATGAA 
GCCAGACTGA AGGAACCCTA CAGACTTGCT CTGAACAGAG TGACAGCCAC TATGGTCAAA 
GAGCAGTCTG ACCTGCAAGG GCAGCTGGGC AGGAGTGACA AGAATGCAGA CTCTGCGCTC 
CCTTCAGCAG AAAGCTCACC ACTTCACATG CAACGAGGGA GGGCAGGAGG AGAGAGCGAG 
ACCAGGCAGC AGTCTTTATC GTCCTCTTCT GGAGCACTTG TGGCACTTAA AATGGATTCG 
GGGGAGTCCA AAACTCCTGT CACGTGCTTT GCTGAAGATT TGGCGACCAC AGTGGTCTCC 
ATGGCTACAG AGCTGGCAGC GATATGCTTG GAGAACTCAA GTGGAAAGCA GCCGTGGTTC 
TGCGCATTAA ATGGTGGCTC GGAAGGTCCA GAAGGGCTGC TTCTGCCCTG CCGCACAGCT 
GCAGCTCTCC GCCGAAAGGA GACACAAAAT GGCACCTCGG TCACCAAGAA GCACAGACCG 
CCACGTCTCA GTGAAATCAA ACGTAAAACC GAAGAACAGC CCGAACTCAT GGAGCGCCTG 
GTCAACCGTG TTGTAGATGA AACCGTTAAC TTAGATGAAC CCACAACCAC TGATCCATTC 
GCGCTCTTTG CATCAGAGGT CACTGCCAGG ATAATGAACT GCCCAGAATT AAGTGTAGTG 
GACACTTCCA AATCTGGGCA GTCGTCCCGC AGCCGCCTGC AGTGTGAGAG ATGGAGTAGC 
AGGGGGAAGG CATCAAGCTA TGAGAGCATT CCAGAGGAGG ATGCAGATCC CTCCGGGACA 
TCAAATACTT TGGGTCCAGG AAACAGGCTT GGGCATAATT TGAGCCGAGG AAGCTCTATC 
TCCAAGCAGT CCAGCTGTGA GAGCATTACG GATGAGTTCT CTCGCTTCAT GGTGAACCAG 
ATGGAGACAG AGGGTCGAGG GTTTGACCTT CTGCTGGACT ACTATGCTGG GAAAAATGCC 
AGCAGCATCC TTGCAGCAGC TGTTCAGCAG GCTGCCAGTA AAAAAAACGG ACACCTCAAT 
GTGCGTACAT CATCTTGTCT CTCGAAGCAG TCCAGCACTG AGAGTATCAC AGAAGAGTTT 
TATCGCTTCA TGCTGAAAGA TATGGACAAG GAAAACAAGG ACTACAGTAT GACCAAGACC 
AAAGAGTGGA GTAATAGCCT GCTACCCCCG TCCCCCAGAA CCCCTTTTTG TATCCGTCAG 
TCTTCTGTTC CTGACAGGCG GTCTTCAGAT TCCAGACTAA CTGTAAACTC GCCTATCAAG 
GCCAATTCAT TCGATGGCTT TGCTCAAAAT GTACACGGGG ACTCGCTTAA CATCTATCCA 
ACTAACTCTG TGTCGTCATC TGGACTCTGC AAGTCCGATT CCTGCTTGTA TAAACGCGGC 
CAAACAGACC AAATCACAGA CATGCTGATT CATGAAACCT GGTCCAGCTC TATCGAATCT 
CTAATGCGCA AGAATAAGAT AATTGCCGAG CCCTCAGAGG ACAGTCTGGA ACTCGATTCT 
GCAGACTCAC AGCCACATGT ACAGCAGTTT GCTAATCGTC TAGCGGCAGA CATCGTAGAG 
AGTGGCAAAT CACAGCTTGG AGGCCAGCAG GATGTCACTG CGGCTGCTTG TCAGCCACAT 
ATTCCAGTTG GAGAAAGGAG ACACGGCTTT AAACATTCCC GTTGCAACAA CAGTGGAAAC 
CGGCCAGGCG TGGAGCATCA GGAAAACAGC TGTAGCTCTT ACAGTTCCTC CTGTGTGAGG 
CAGGCATGGC GCGGACAGAG AGAGGTACCC TTGATTCACA TTGAACCAGA TCAAAGAGAA 
GAGGCCTCGG AAGAGAAGGG AGGGGTTGAG ACCCACCACA GGGAGGCATC CCATCAGACC 
CAGCAACAAA GTGGCAAAGG GAGTGAGACA GCAACCAAAA GCAGCAGTGA CTCAGACAGG 
GTCTCCTCAG TGGCCACAGT GCCCCCTGCT GGTGTGGAGG TGGAGCGGCG GTCTCTCAGC 
GCTAGCAGTG AGGAAAGTGG CTCAGGCAGC TGGGCACAGA TTGCCACTGA AGACGACCCC 
CAGGAGGAGA CCACCAGTAG CTTTATCCAG CTAAGCGAGG GAAATGGGAA CAGCAGCACT 
TCTAGTCTGG GACTGGCCGA CCTAGAGGGC TTTCCGGACT TCTCCATCTC CAGCAACCTA 
ATCAGTGAGG AGAGGGAGAG GAAGACTTCG CACTGTCAGG ACCAGGCGGA CGAGGTGACG 
TCGGGCTTGT CGACGGCCGG CAGCAGCTGT CAGCGGGAGC TCCTGGTGCT GAACTGTGAC 
CTGGAGCCGG ACTGTGTGGA CGGAGAGCTG AGAGCCGCGC TGCAGTGGAT CTCAGCATCT 
GAACTCGGCG TCCCGGCGCT TTACTTCAGA AAGACTCACC AGCACAATCT TACAAAGCTC 
CACAGAGTGC TGCAGTTGGC AGGTCAGAAG GCTTGGCGTG TTGGAGACTT GTTCAGCGCC 
GTGGCTCAGT TCTGTCAGCT CCACCAGGAC CTGGAGCTGA AAGGACGGCC GCTGCCCAGC 
CTGTTTGACT GGATCCTGAA GACCAAACGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292131.1
CDS	436..5148
Translation	MLANLLSVLQNFTESNFQSSAMFEGSESANTQEVKSESTLGSSLSACKKVLCSNSVLDSSEYWLKNDKTLCRIGFLEDQHDSGCPTICFVNLDRHTSDWHDDNYIKKLASVCPELPRLIESLGVRQPKENEILLLSSLEPPDSCQHDPSHPQSPRTADVCLVHCSCGRRPTQTSSIIFEINKFLIGLQSGQERQKHGRLAGQRAAEDDTNRSVSSIEEDFLTASEQLGEDSEEDPFRNDPENSLMPESVKVLRAAHAQKRSEIEREDSEDSETLCTSSNSQKLSRTYSAPARGRPTTPKQVKESAGHYATNLAESVLQDAFIRLSQDEPSFVAEAAVSVSSDISHSTEAPQRARACSFELPKIVIVQSPDNSEEVTEWPEVQSHATSEHDSGNKAKAKHGTHPPHNSPVGHPAKPLEIALACAASVIGTITTPQVTEQLTLESGEEYECEDEEEESETDQGEYSFSSAVCGMSQVAGAVAAVDLADDSGDNEDLADMYSASMGLLSVAQASTAIPLHCSIAEGTSVEAFRANVAEVLLREASAVFTHRQSYSSVANFLETTHNKIVDGITNPRRPYQEQQDVDNFTQEISDSIFQYALEKAEKRKELEGPGKDAPNIEGFLSDCVNNLLFDVLCVTSRKISDISNCNMESCDGQEGSVGYRAYEADSKAGREALSQLQHLIEPSQAYNHGERRSSVEKLSALIGKREREQKHIQEERENEARLKEPYRLALNRVTATMVKEQSDLQGQLGRSDKNADSALPSAESSPLHMQRGRAGGESETRQQSLSSSSGALVALKMDSGESKTPVTCFAEDLATTVVSMATELAAICLENSSGKQPWFCALNGGSEGPEGLLLPCRTAAALRRKETQNGTSVTKKHRPPRLSEIKRKTEEQPELMERLVNRVVDETVNLDEPTTTDPFALFASEVTARIMNCPELSVVDTSKSGQSSRSRLQCERWSSRGKASSYESIPEEDADPSGTSNTLGPGNRLGHNLSRGSSISKQSSCESITDEFSRFMVNQMETEGRGFDLLLDYYAGKNASSILAAAVQQAASKKNGHLNVRTSSCLSKQSSTESITEEFYRFMLKDMDKENKDYSMTKTKEWSNSLLPPSPRTPFCIRQSSVPDRRSSDSRLTVNSPIKANSFDGFAQNVHGDSLNIYPTNSVSSSGLCKSDSCLYKRGQTDQITDMLIHETWSSSIESLMRKNKIIAEPSEDSLELDSADSQPHVQQFANRLAADIVESGKSQLGGQQDVTAAACQPHIPVGERRHGFKHSRCNNSGNRPGVEHQENSCSSYSSSCVRQAWRGQREVPLIHIEPDQREEASEEKGGVETHHREASHQTQQQSGKGSETATKSSSDSDRVSSVATVPPAGVEVERRSLSASSEESGSGSWAQIATEDDPQEETTSSFIQLSEGNGNSSTSSLGLADLEGFPDFSISSNLISEERERKTSHCQDQADEVTSGLSTAGSSCQRELLVLNCDLEPDCVDGELRAALQWISASELGVPALYFRKTHQHNLTKLHRVLQLAGQKAWRVGDLFSAVAQFCQLHQDLELKGRPLPSLFDWILKTKR

Target ORF information:

RefSeq Version	XM_009293856.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293856.3

ORF Insert Sequence:

ATGCTAGCAA ACCTGCTCAG CGTCCTCCAA AACTTCACAG AGAGTAATTT CCAATCATCT 
GCCATGTTTG AGGGATCTGA GTCCGCGAAC ACACAGGAAG TCAAATCTGA GAGCACCCTT 
GGGTCCTCGC TCTCCGCCTG CAAGAAGGTT TTGTGCAGTA ACAGTGTTCT GGATTCGTCT 
GAGTACTGGC TGAAGAATGA TAAGACGCTC TGCAGAATTG GATTTCTGGA GGACCAGCAT 
GACAGCGGCT GCCCGACAAT TTGCTTTGTA AACCTGGACA GACATACTTC AGATTGGCAT 
GATGACAACT ACATCAAGAA GCTGGCCTCG GTGTGCCCAG AGCTGCCACG GTTAATTGAG 
TCTCTCGGAG TGCGGCAACC CAAAGAAAAT GAGATTCTGC TTCTCAGCAG CCTAGAGCCA 
CCGGACTCCT GCCAGCATGA CCCCAGCCAC CCACAGAGCC CGAGAACAGC GGATGTCTGT 
CTGGTGCATT GTTCATGTGG ACGCCGTCCC ACCCAAACGA GCAGTATCAT TTTTGAGATT 
AACAAGTTCC TGATTGGACT ACAGTCAGGG CAGGAGAGGC AGAAGCATGG CCGATTGGCT 
GGACAACGAG CTGCTGAAGA TGACACCAAT CGCTCTGTTT CGTCGATTGA AGAAGATTTC 
CTCACTGCCT CAGAGCAGCT TGGGGAAGAC AGCGAGGAAG ACCCTTTCAG GAATGATCCT 
GAGAATTCTC TCATGCCCGA GTCTGTTAAG GTGTTGAGAG CAGCCCACGC TCAAAAAAGG 
AGTGAAATAG AGAGAGAGGA CAGTGAGGAC TCTGAAACCC TCTGCACCTC TTCCAACTCC 
CAAAAGCTTT CAAGAACATA TTCCGCCCCT GCAAGAGGCC GACCCACCAC CCCAAAACAA 
GTCAAGGAGT CAGCCGGTCA CTACGCCACC AATCTGGCTG AGTCAGTCCT GCAAGATGCT 
TTTATTCGTT TGTCACAAGA TGAGCCATCA TTTGTTGCAG AAGCAGCAGT GAGTGTGTCA 
AGTGATATAA GCCATTCCAC TGAGGCACCA CAGAGGGCTC GAGCTTGCTC ATTCGAACTT 
CCTAAAATTG TGATTGTCCA AAGTCCTGAT AACAGTGAGG AGGTAACAGA ATGGCCGGAA 
GTGCAGTCAC ATGCAACATC AGAGCATGAT AGTGGAAATA AGGCCAAAGC AAAGCATGGT 
ACTCATCCCC CGCACAACTC GCCAGTTGGA CACCCTGCTA AGCCATTGGA AATAGCTTTG 
GCATGTGCTG CAAGTGTCAT TGGTACCATT ACCACCCCTC AAGTCACAGA ACAACTCACA 
CTAGAGTCAG GAGAGGAATA TGAGTGTGAA GATGAAGAGG AAGAAAGTGA AACTGATCAA 
GGGGAGTATT CGTTTTCATC TGCGGTATGT GGCATGTCTC AGGTCGCTGG AGCTGTAGCT 
GCGGTCGATC TAGCAGATGA CTCTGGGGAC AATGAAGACT TGGCAGACAT GTATTCCGCC 
TCTATGGGAC TTCTGTCAGT TGCCCAAGCT TCAACTGCAA TACCTCTTCA CTGTAGCATA 
GCAGAGGGCA CCAGCGTTGA GGCCTTTCGA GCCAATGTGG CTGAAGTTCT CCTCAGGGAA 
GCTTCAGCTG TATTCACTCA CAGACAAAGT TATTCAAGTG TTGCAAATTT CCTTGAGACC 
ACACATAACA AGATAGTGGA TGGCATCACA AATCCAAGAA GACCGTATCA GGAGCAACAA 
GATGTGGATA ACTTCACTCA AGAAATATCT GACAGTATCT TTCAGTATGC CCTTGAGAAG 
GCGGAGAAGA GAAAAGAACT TGAGGGTCCT GGAAAAGATG CCCCTAACAT TGAGGGTTTC 
CTCTCGGATT GTGTGAATAA TTTGCTCTTT GATGTACTGT GTGTAACATC AAGGAAAATA 
AGTGACATTT CAAATTGTAA TATGGAATCA TGTGATGGGC AGGAAGGCAG TGTCGGCTAT 
AGGGCATATG AGGCTGATTC CAAAGCTGGC AGGGAAGCAT TGAGCCAATT GCAGCATTTG 
ATTGAGCCAA GCCAGGCTTA TAATCATGGT GAAAGGCGAA GCAGTGTGGA GAAGTTATCT 
GCTCTAATAG GAAAGAGAGA GAGAGAGCAG AAACACATAC AGGAGGAAAG AGAAAATGAA 
GCCAGACTGA AGGAACCCTA CAGACTTGCT CTGAACAGAG TGACAGCCAC TATGGTCAAA 
GAGCAGTCTG ACCTGCAAGG GCAGCTGGGC AGGAGTGACA AGAATGCAGA CTCTGCGCTC 
CCTTCAGCAG AAAGCTCACC ACTTCACATG CAACGAGGGA GGGCAGGAGG AGAGAGCGAG 
ACCAGGCAGC AGTCTTTATC GTCCTCTTCT GGAGCACTTG TGGCACTTAA AATGGATTCG 
GGGGAGTCCA AAACTCCTGT CACGTGCTTT GCTGAAGATT TGGCGACCAC AGTGGTCTCC 
ATGGCTACAG AGCTGGCAGC GATATGCTTG GAGAACTCAA GTGGAAAGCA GCCGTGGTTC 
TGCGCATTAA ATGGTGGCTC GGAAGGTCCA GAAGGGCTGC TTCTGCCCTG CCGCACAGCT 
GCAGCTCTCC GCCGAAAGGA GACACAAAAT GGCACCTCGG TCACCAAGAA GCACAGACCG 
CCACGTCTCA GTGAAATCAA ACGTAAAACC GAAGAACAGC CCGAACTCAT GGAGCGCCTG 
GTCAACCGTG TTGTAGATGA AACCGTTAAC TTAGATGAAC CCACAACCAC TGATCCATTC 
GCGCTCTTTG CATCAGAGGT CACTGCCAGG ATAATGAACT GCCCAGAATT AAGTGTAGTG 
GACACTTCCA AATCTGGGCA GTCGTCCCGC AGCCGCCTGC AGTGTGAGAG ATGGAGTAGC 
AGGGGGAAGG CATCAAGCTA TGAGAGCATT CCAGAGGAGG ATGCAGATCC CTCCGGGACA 
TCAAATACTT TGGGTCCAGG AAACAGGCTT GGGCATAATT TGAGCCGAGG AAGCTCTATC 
TCCAAGCAGT CCAGCTGTGA GAGCATTACG GATGAGTTCT CTCGCTTCAT GGTGAACCAG 
ATGGAGACAG AGGGTCGAGG GTTTGACCTT CTGCTGGACT ACTATGCTGG GAAAAATGCC 
AGCAGCATCC TTGCAGCAGC TGTTCAGCAG GCTGCCAGTA AAAAAAACGG ACACCTCAAT 
GTGCGTACAT CATCTTGTCT CTCGAAGCAG TCCAGCACTG AGAGTATCAC AGAAGAGTTT 
TATCGCTTCA TGCTGAAAGA TATGGACAAG GAAAACAAGG ACTACAGTAT GACCAAGACC 
AAAGAGTGGA GTAATAGCCT GCTACCCCCG TCCCCCAGAA CCCCTTTTTG TATCCGTCAG 
TCTTCTGTTC CTGACAGGCG GTCTTCAGAT TCCAGACTAA CTGTAAACTC GCCTATCAAG 
GCCAATTCAT TCGATGGCTT TGCTCAAAAT GTACACGGGG ACTCGCTTAA CATCTATCCA 
ACTAACTCTG TGTCGTCATC TGGACTCTGC AAGTCCGATT CCTGCTTGTA TAAACGCGGC 
CAAACAGACC AAATCACAGA CATGCTGATT CATGAAACCT GGTCCAGCTC TATCGAATCT 
CTAATGCGCA AGAATAAGAT AATTGCCGAG CCCTCAGAGG ACAGTCTGGA ACTCGATTCT 
GCAGACTCAC AGCCACATGT ACAGCAGTTT GCTAATCGTC TAGCGGCAGA CATCGTAGAG 
AGTGGCAAAT CACAGCTTGG AGGCCAGCAG GATGTCACTG CGGCTGCTTG TCAGCCACAT 
ATTCCAGTTG GAGAAAGGAG ACACGGCTTT AAACATTCCC GTTGCAACAA CAGTGGAAAC 
CGGCCAGGCG TGGAGCATCA GGAAAACAGC TGTAGCTCTT ACAGTTCCTC CTGTGTGAGG 
CAGGCATGGC GCGGACAGAG AGAGGTACCC TTGATTCACA TTGAACCAGA TCAAAGAGAA 
GAGGCCTCGG AAGAGAAGGG AGGGGTTGAG ACCCACCACA GGGAGGCATC CCATCAGACC 
CAGCAACAAA GTGGCAAAGG GAGTGAGACA GCAACCAAAA GCAGCAGTGA CTCAGACAGG 
GTCTCCTCAG TGGCCACAGT GCCCCCTGCT GGTGTGGAGG TGGAGCGGCG GTCTCTCAGC 
GCTAGCAGTG AGGAAAGTGG CTCAGGCAGC TGGGCACAGA TTGCCACTGA AGACGACCCC 
CAGGAGGAGA CCACCAGTAG CTTTATCCAG CTAAGCGAGG GAAATGGGAA CAGCAGCACT 
TCTAGTCTGG GACTGGCCGA CCTAGAGGGC TTTCCGGACT TCTCCATCTC CAGCAACCTA 
ATCAGTGAGG AGAGGGAGAG GAAGACTTCG CACTGTCAGG ACCAGGCGGA CGAGGTGACG 
TCGGGCTTGT CGACGGCCGG CAGCAGCTGT CAGCGGGAGC TCCTGGTGCT GAACTGTGAC 
CTGGAGCCGG ACTGTGTGGA CGGAGAGCTG AGAGCCGCGC TGCAGTGGAT CTCAGCATCT 
GAACTCGGCG TCCCGGCGCT TTACTTCAGA AAGACTCACC AGCACAATCT TACAAAGCTC 
CACAGAGTGC TGCAGTTGGC AGGTCAGAAG GCTTGGCGTG TTGGAGACTT GTTCAGCGCC 
GTGGCTCAGT TCTGTCAGCT CCACCAGGAC CTGGAGCTGA AAGGACGGCC GCTGCCCAGC 
CTGTTTGACT GGATCCTGAA GACCAAACGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sphkap ( XM_009293856.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292131.1 Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48351
Clone ID Related Accession (Same CDS sequence)	XM_009293856.3 , XM_009293856.2
Accession Version	XM_009293856.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGCAA ACCTGCTCAG CGTCCTCCAA AACTTCACAG AGAGTAATTT CCAATCATCT 
GCCATGTTTG AGGGATCTGA GTCCGCGAAC ACACAGGAAG TCAAATCTGA GAGCACCCTT 
GGGTCCTCGC TCTCCGCCTG CAAGAAGGTT TTGTGCAGTA ACAGTGTTCT GGATTCGTCT 
GAGTACTGGC TGAAGAATGA TAAGACGCTC TGCAGAATTG GATTTCTGGA GGACCAGCAT 
GACAGCGGCT GCCCGACAAT TTGCTTTGTA AACCTGGACA GACATACTTC AGATTGGCAT 
GATGACAACT ACATCAAGAA GCTGGCCTCG GTGTGCCCAG AGCTGCCACG GTTAATTGAG 
TCTCTCGGAG TGCGGCAACC CAAAGAAAAT GAGATTCTGC TTCTCAGCAG CCTAGAGCCA 
CCGGACTCCT GCCAGCATGA CCCCAGCCAC CCACAGAGCC CGAGAACAGC GGATGTCTGT 
CTGGTGCATT GTTCATGTGG ACGCCGTCCC ACCCAAACGA GCAGTATCAT TTTTGAGATT 
AACAAGTTCC TGATTGGACT ACAGTCAGGG CAGGAGAGGC AGAAGCATGG CCGATTGGCT 
GGACAACGAG CTGCTGAAGA TGACACCAAT CGCTCTGTTT CGTCGATTGA AGAAGATTTC 
CTCACTGCCT CAGAGCAGCT TGGGGAAGAC AGCGAGGAAG ACCCTTTCAG GAATGATCCT 
GAGAATTCTC TCATGCCCGA GTCTGTTAAG GTGTTGAGAG CAGCCCACGC TCAAAAAAGG 
AGTGAAATAG AGAGAGAGGA CAGTGAGGAC TCTGAAACCC TCTGCACCTC TTCCAACTCC 
CAAAAGCTTT CAAGAACATA TTCCGCCCCT GCAAGAGGCC GACCCACCAC CCCAAAACAA 
GTCAAGGAGT CAGCCGGTCA CTACGCCACC AATCTGGCTG AGTCAGTCCT GCAAGATGCT 
TTTATTCGTT TGTCACAAGA TGAGCCATCA TTTGTTGCAG AAGCAGCAGT GAGTGTGTCA 
AGTGATATAA GCCATTCCAC TGAGGCACCA CAGAGGGCTC GAGCTTGCTC ATTCGAACTT 
CCTAAAATTG TGATTGTCCA AAGTCCTGAT AACAGTGAGG AGGTAACAGA ATGGCCGGAA 
GTGCAGTCAC ATGCAACATC AGAGCATGAT AGTGGAAATA AGGCCAAAGC AAAGCATGGT 
ACTCATCCCC CGCACAACTC GCCAGTTGGA CACCCTGCTA AGCCATTGGA AATAGCTTTG 
GCATGTGCTG CAAGTGTCAT TGGTACCATT ACCACCCCTC AAGTCACAGA ACAACTCACA 
CTAGAGTCAG GAGAGGAATA TGAGTGTGAA GATGAAGAGG AAGAAAGTGA AACTGATCAA 
GGGGAGTATT CGTTTTCATC TGCGGTATGT GGCATGTCTC AGGTCGCTGG AGCTGTAGCT 
GCGGTCGATC TAGCAGATGA CTCTGGGGAC AATGAAGACT TGGCAGACAT GTATTCCGCC 
TCTATGGGAC TTCTGTCAGT TGCCCAAGCT TCAACTGCAA TACCTCTTCA CTGTAGCATA 
GCAGAGGGCA CCAGCGTTGA GGCCTTTCGA GCCAATGTGG CTGAAGTTCT CCTCAGGGAA 
GCTTCAGCTG TATTCACTCA CAGACAAAGT TATTCAAGTG TTGCAAATTT CCTTGAGACC 
ACACATAACA AGATAGTGGA TGGCATCACA AATCCAAGAA GACCGTATCA GGAGCAACAA 
GATGTGGATA ACTTCACTCA AGAAATATCT GACAGTATCT TTCAGTATGC CCTTGAGAAG 
GCGGAGAAGA GAAAAGAACT TGAGGGTCCT GGAAAAGATG CCCCTAACAT TGAGGGTTTC 
CTCTCGGATT GTGTGAATAA TTTGCTCTTT GATGTACTGT GTGTAACATC AAGGAAAATA 
AGTGACATTT CAAATTGTAA TATGGAATCA TGTGATGGGC AGGAAGGCAG TGTCGGCTAT 
AGGGCATATG AGGCTGATTC CAAAGCTGGC AGGGAAGCAT TGAGCCAATT GCAGCATTTG 
ATTGAGCCAA GCCAGGCTTA TAATCATGGT GAAAGGCGAA GCAGTGTGGA GAAGTTATCT 
GCTCTAATAG GAAAGAGAGA GAGAGAGCAG AAACACATAC AGGAGGAAAG AGAAAATGAA 
GCCAGACTGA AGGAACCCTA CAGACTTGCT CTGAACAGAG TGACAGCCAC TATGGTCAAA 
GAGCAGTCTG ACCTGCAAGG GCAGCTGGGC AGGAGTGACA AGAATGCAGA CTCTGCGCTC 
CCTTCAGCAG AAAGCTCACC ACTTCACATG CAACGAGGGA GGGCAGGAGG AGAGAGCGAG 
ACCAGGCAGC AGTCTTTATC GTCCTCTTCT GGAGCACTTG TGGCACTTAA AATGGATTCG 
GGGGAGTCCA AAACTCCTGT CACGTGCTTT GCTGAAGATT TGGCGACCAC AGTGGTCTCC 
ATGGCTACAG AGCTGGCAGC GATATGCTTG GAGAACTCAA GTGGAAAGCA GCCGTGGTTC 
TGCGCATTAA ATGGTGGCTC GGAAGGTCCA GAAGGGCTGC TTCTGCCCTG CCGCACAGCT 
GCAGCTCTCC GCCGAAAGGA GACACAAAAT GGCACCTCGG TCACCAAGAA GCACAGACCG 
CCACGTCTCA GTGAAATCAA ACGTAAAACC GAAGAACAGC CCGAACTCAT GGAGCGCCTG 
GTCAACCGTG TTGTAGATGA AACCGTTAAC TTAGATGAAC CCACAACCAC TGATCCATTC 
GCGCTCTTTG CATCAGAGGT CACTGCCAGG ATAATGAACT GCCCAGAATT AAGTGTAGTG 
GACACTTCCA AATCTGGGCA GTCGTCCCGC AGCCGCCTGC AGTGTGAGAG ATGGAGTAGC 
AGGGGGAAGG CATCAAGCTA TGAGAGCATT CCAGAGGAGG ATGCAGATCC CTCCGGGACA 
TCAAATACTT TGGGTCCAGG AAACAGGCTT GGGCATAATT TGAGCCGAGG AAGCTCTATC 
TCCAAGCAGT CCAGCTGTGA GAGCATTACG GATGAGTTCT CTCGCTTCAT GGTGAACCAG 
ATGGAGACAG AGGGTCGAGG GTTTGACCTT CTGCTGGACT ACTATGCTGG GAAAAATGCC 
AGCAGCATCC TTGCAGCAGC TGTTCAGCAG GCTGCCAGTA AAAAAAACGG ACACCTCAAT 
GTGCGTACAT CATCTTGTCT CTCGAAGCAG TCCAGCACTG AGAGTATCAC AGAAGAGTTT 
TATCGCTTCA TGCTGAAAGA TATGGACAAG GAAAACAAGG ACTACAGTAT GACCAAGACC 
AAAGAGTGGA GTAATAGCCT GCTACCCCCG TCCCCCAGAA CCCCTTTTTG TATCCGTCAG 
TCTTCTGTTC CTGACAGGCG GTCTTCAGAT TCCAGACTAA CTGTAAACTC GCCTATCAAG 
GCCAATTCAT TCGATGGCTT TGCTCAAAAT GTACACGGGG ACTCGCTTAA CATCTATCCA 
ACTAACTCTG TGTCGTCATC TGGACTCTGC AAGTCCGATT CCTGCTTGTA TAAACGCGGC 
CAAACAGACC AAATCACAGA CATGCTGATT CATGAAACCT GGTCCAGCTC TATCGAATCT 
CTAATGCGCA AGAATAAGAT AATTGCCGAG CCCTCAGAGG ACAGTCTGGA ACTCGATTCT 
GCAGACTCAC AGCCACATGT ACAGCAGTTT GCTAATCGTC TAGCGGCAGA CATCGTAGAG 
AGTGGCAAAT CACAGCTTGG AGGCCAGCAG GATGTCACTG CGGCTGCTTG TCAGCCACAT 
ATTCCAGTTG GAGAAAGGAG ACACGGCTTT AAACATTCCC GTTGCAACAA CAGTGGAAAC 
CGGCCAGGCG TGGAGCATCA GGAAAACAGC TGTAGCTCTT ACAGTTCCTC CTGTGTGAGG 
CAGGCATGGC GCGGACAGAG AGAGGTACCC TTGATTCACA TTGAACCAGA TCAAAGAGAA 
GAGGCCTCGG AAGAGAAGGG AGGGGTTGAG ACCCACCACA GGGAGGCATC CCATCAGACC 
CAGCAACAAA GTGGCAAAGG GAGTGAGACA GCAACCAAAA GCAGCAGTGA CTCAGACAGG 
GTCTCCTCAG TGGCCACAGT GCCCCCTGCT GGTGTGGAGG TGGAGCGGCG GTCTCTCAGC 
GCTAGCAGTG AGGAAAGTGG CTCAGGCAGC TGGGCACAGA TTGCCACTGA AGACGACCCC 
CAGGAGGAGA CCACCAGTAG CTTTATCCAG CTAAGCGAGG GAAATGGGAA CAGCAGCACT 
TCTAGTCTGG GACTGGCCGA CCTAGAGGGC TTTCCGGACT TCTCCATCTC CAGCAACCTA 
ATCAGTGAGG AGAGGGAGAG GAAGACTTCG CACTGTCAGG ACCAGGCGGA CGAGGTGACG 
TCGGGCTTGT CGACGGCCGG CAGCAGCTGT CAGCGGGAGC TCCTGGTGCT GAACTGTGAC 
CTGGAGCCGG ACTGTGTGGA CGGAGAGCTG AGAGCCGCGC TGCAGTGGAT CTCAGCATCT 
GAACTCGGCG TCCCGGCGCT TTACTTCAGA AAGACTCACC AGCACAATCT TACAAAGCTC 
CACAGAGTGC TGCAGTTGGC AGGTCAGAAG GCTTGGCGTG TTGGAGACTT GTTCAGCGCC 
GTGGCTCAGT TCTGTCAGCT CCACCAGGAC CTGGAGCTGA AAGGACGGCC GCTGCCCAGC 
CTGTTTGACT GGATCCTGAA GACCAAACGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292131.1
CDS	449..5161
Translation	MLANLLSVLQNFTESNFQSSAMFEGSESANTQEVKSESTLGSSLSACKKVLCSNSVLDSSEYWLKNDKTLCRIGFLEDQHDSGCPTICFVNLDRHTSDWHDDNYIKKLASVCPELPRLIESLGVRQPKENEILLLSSLEPPDSCQHDPSHPQSPRTADVCLVHCSCGRRPTQTSSIIFEINKFLIGLQSGQERQKHGRLAGQRAAEDDTNRSVSSIEEDFLTASEQLGEDSEEDPFRNDPENSLMPESVKVLRAAHAQKRSEIEREDSEDSETLCTSSNSQKLSRTYSAPARGRPTTPKQVKESAGHYATNLAESVLQDAFIRLSQDEPSFVAEAAVSVSSDISHSTEAPQRARACSFELPKIVIVQSPDNSEEVTEWPEVQSHATSEHDSGNKAKAKHGTHPPHNSPVGHPAKPLEIALACAASVIGTITTPQVTEQLTLESGEEYECEDEEEESETDQGEYSFSSAVCGMSQVAGAVAAVDLADDSGDNEDLADMYSASMGLLSVAQASTAIPLHCSIAEGTSVEAFRANVAEVLLREASAVFTHRQSYSSVANFLETTHNKIVDGITNPRRPYQEQQDVDNFTQEISDSIFQYALEKAEKRKELEGPGKDAPNIEGFLSDCVNNLLFDVLCVTSRKISDISNCNMESCDGQEGSVGYRAYEADSKAGREALSQLQHLIEPSQAYNHGERRSSVEKLSALIGKREREQKHIQEERENEARLKEPYRLALNRVTATMVKEQSDLQGQLGRSDKNADSALPSAESSPLHMQRGRAGGESETRQQSLSSSSGALVALKMDSGESKTPVTCFAEDLATTVVSMATELAAICLENSSGKQPWFCALNGGSEGPEGLLLPCRTAAALRRKETQNGTSVTKKHRPPRLSEIKRKTEEQPELMERLVNRVVDETVNLDEPTTTDPFALFASEVTARIMNCPELSVVDTSKSGQSSRSRLQCERWSSRGKASSYESIPEEDADPSGTSNTLGPGNRLGHNLSRGSSISKQSSCESITDEFSRFMVNQMETEGRGFDLLLDYYAGKNASSILAAAVQQAASKKNGHLNVRTSSCLSKQSSTESITEEFYRFMLKDMDKENKDYSMTKTKEWSNSLLPPSPRTPFCIRQSSVPDRRSSDSRLTVNSPIKANSFDGFAQNVHGDSLNIYPTNSVSSSGLCKSDSCLYKRGQTDQITDMLIHETWSSSIESLMRKNKIIAEPSEDSLELDSADSQPHVQQFANRLAADIVESGKSQLGGQQDVTAAACQPHIPVGERRHGFKHSRCNNSGNRPGVEHQENSCSSYSSSCVRQAWRGQREVPLIHIEPDQREEASEEKGGVETHHREASHQTQQQSGKGSETATKSSSDSDRVSSVATVPPAGVEVERRSLSASSEESGSGSWAQIATEDDPQEETTSSFIQLSEGNGNSSTSSLGLADLEGFPDFSISSNLISEERERKTSHCQDQADEVTSGLSTAGSSCQRELLVLNCDLEPDCVDGELRAALQWISASELGVPALYFRKTHQHNLTKLHRVLQLAGQKAWRVGDLFSAVAQFCQLHQDLELKGRPLPSLFDWILKTKR

Target ORF information:

RefSeq Version	XM_009293856.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293856.2

ORF Insert Sequence:

ATGCTAGCAA ACCTGCTCAG CGTCCTCCAA AACTTCACAG AGAGTAATTT CCAATCATCT 
GCCATGTTTG AGGGATCTGA GTCCGCGAAC ACACAGGAAG TCAAATCTGA GAGCACCCTT 
GGGTCCTCGC TCTCCGCCTG CAAGAAGGTT TTGTGCAGTA ACAGTGTTCT GGATTCGTCT 
GAGTACTGGC TGAAGAATGA TAAGACGCTC TGCAGAATTG GATTTCTGGA GGACCAGCAT 
GACAGCGGCT GCCCGACAAT TTGCTTTGTA AACCTGGACA GACATACTTC AGATTGGCAT 
GATGACAACT ACATCAAGAA GCTGGCCTCG GTGTGCCCAG AGCTGCCACG GTTAATTGAG 
TCTCTCGGAG TGCGGCAACC CAAAGAAAAT GAGATTCTGC TTCTCAGCAG CCTAGAGCCA 
CCGGACTCCT GCCAGCATGA CCCCAGCCAC CCACAGAGCC CGAGAACAGC GGATGTCTGT 
CTGGTGCATT GTTCATGTGG ACGCCGTCCC ACCCAAACGA GCAGTATCAT TTTTGAGATT 
AACAAGTTCC TGATTGGACT ACAGTCAGGG CAGGAGAGGC AGAAGCATGG CCGATTGGCT 
GGACAACGAG CTGCTGAAGA TGACACCAAT CGCTCTGTTT CGTCGATTGA AGAAGATTTC 
CTCACTGCCT CAGAGCAGCT TGGGGAAGAC AGCGAGGAAG ACCCTTTCAG GAATGATCCT 
GAGAATTCTC TCATGCCCGA GTCTGTTAAG GTGTTGAGAG CAGCCCACGC TCAAAAAAGG 
AGTGAAATAG AGAGAGAGGA CAGTGAGGAC TCTGAAACCC TCTGCACCTC TTCCAACTCC 
CAAAAGCTTT CAAGAACATA TTCCGCCCCT GCAAGAGGCC GACCCACCAC CCCAAAACAA 
GTCAAGGAGT CAGCCGGTCA CTACGCCACC AATCTGGCTG AGTCAGTCCT GCAAGATGCT 
TTTATTCGTT TGTCACAAGA TGAGCCATCA TTTGTTGCAG AAGCAGCAGT GAGTGTGTCA 
AGTGATATAA GCCATTCCAC TGAGGCACCA CAGAGGGCTC GAGCTTGCTC ATTCGAACTT 
CCTAAAATTG TGATTGTCCA AAGTCCTGAT AACAGTGAGG AGGTAACAGA ATGGCCGGAA 
GTGCAGTCAC ATGCAACATC AGAGCATGAT AGTGGAAATA AGGCCAAAGC AAAGCATGGT 
ACTCATCCCC CGCACAACTC GCCAGTTGGA CACCCTGCTA AGCCATTGGA AATAGCTTTG 
GCATGTGCTG CAAGTGTCAT TGGTACCATT ACCACCCCTC AAGTCACAGA ACAACTCACA 
CTAGAGTCAG GAGAGGAATA TGAGTGTGAA GATGAAGAGG AAGAAAGTGA AACTGATCAA 
GGGGAGTATT CGTTTTCATC TGCGGTATGT GGCATGTCTC AGGTCGCTGG AGCTGTAGCT 
GCGGTCGATC TAGCAGATGA CTCTGGGGAC AATGAAGACT TGGCAGACAT GTATTCCGCC 
TCTATGGGAC TTCTGTCAGT TGCCCAAGCT TCAACTGCAA TACCTCTTCA CTGTAGCATA 
GCAGAGGGCA CCAGCGTTGA GGCCTTTCGA GCCAATGTGG CTGAAGTTCT CCTCAGGGAA 
GCTTCAGCTG TATTCACTCA CAGACAAAGT TATTCAAGTG TTGCAAATTT CCTTGAGACC 
ACACATAACA AGATAGTGGA TGGCATCACA AATCCAAGAA GACCGTATCA GGAGCAACAA 
GATGTGGATA ACTTCACTCA AGAAATATCT GACAGTATCT TTCAGTATGC CCTTGAGAAG 
GCGGAGAAGA GAAAAGAACT TGAGGGTCCT GGAAAAGATG CCCCTAACAT TGAGGGTTTC 
CTCTCGGATT GTGTGAATAA TTTGCTCTTT GATGTACTGT GTGTAACATC AAGGAAAATA 
AGTGACATTT CAAATTGTAA TATGGAATCA TGTGATGGGC AGGAAGGCAG TGTCGGCTAT 
AGGGCATATG AGGCTGATTC CAAAGCTGGC AGGGAAGCAT TGAGCCAATT GCAGCATTTG 
ATTGAGCCAA GCCAGGCTTA TAATCATGGT GAAAGGCGAA GCAGTGTGGA GAAGTTATCT 
GCTCTAATAG GAAAGAGAGA GAGAGAGCAG AAACACATAC AGGAGGAAAG AGAAAATGAA 
GCCAGACTGA AGGAACCCTA CAGACTTGCT CTGAACAGAG TGACAGCCAC TATGGTCAAA 
GAGCAGTCTG ACCTGCAAGG GCAGCTGGGC AGGAGTGACA AGAATGCAGA CTCTGCGCTC 
CCTTCAGCAG AAAGCTCACC ACTTCACATG CAACGAGGGA GGGCAGGAGG AGAGAGCGAG 
ACCAGGCAGC AGTCTTTATC GTCCTCTTCT GGAGCACTTG TGGCACTTAA AATGGATTCG 
GGGGAGTCCA AAACTCCTGT CACGTGCTTT GCTGAAGATT TGGCGACCAC AGTGGTCTCC 
ATGGCTACAG AGCTGGCAGC GATATGCTTG GAGAACTCAA GTGGAAAGCA GCCGTGGTTC 
TGCGCATTAA ATGGTGGCTC GGAAGGTCCA GAAGGGCTGC TTCTGCCCTG CCGCACAGCT 
GCAGCTCTCC GCCGAAAGGA GACACAAAAT GGCACCTCGG TCACCAAGAA GCACAGACCG 
CCACGTCTCA GTGAAATCAA ACGTAAAACC GAAGAACAGC CCGAACTCAT GGAGCGCCTG 
GTCAACCGTG TTGTAGATGA AACCGTTAAC TTAGATGAAC CCACAACCAC TGATCCATTC 
GCGCTCTTTG CATCAGAGGT CACTGCCAGG ATAATGAACT GCCCAGAATT AAGTGTAGTG 
GACACTTCCA AATCTGGGCA GTCGTCCCGC AGCCGCCTGC AGTGTGAGAG ATGGAGTAGC 
AGGGGGAAGG CATCAAGCTA TGAGAGCATT CCAGAGGAGG ATGCAGATCC CTCCGGGACA 
TCAAATACTT TGGGTCCAGG AAACAGGCTT GGGCATAATT TGAGCCGAGG AAGCTCTATC 
TCCAAGCAGT CCAGCTGTGA GAGCATTACG GATGAGTTCT CTCGCTTCAT GGTGAACCAG 
ATGGAGACAG AGGGTCGAGG GTTTGACCTT CTGCTGGACT ACTATGCTGG GAAAAATGCC 
AGCAGCATCC TTGCAGCAGC TGTTCAGCAG GCTGCCAGTA AAAAAAACGG ACACCTCAAT 
GTGCGTACAT CATCTTGTCT CTCGAAGCAG TCCAGCACTG AGAGTATCAC AGAAGAGTTT 
TATCGCTTCA TGCTGAAAGA TATGGACAAG GAAAACAAGG ACTACAGTAT GACCAAGACC 
AAAGAGTGGA GTAATAGCCT GCTACCCCCG TCCCCCAGAA CCCCTTTTTG TATCCGTCAG 
TCTTCTGTTC CTGACAGGCG GTCTTCAGAT TCCAGACTAA CTGTAAACTC GCCTATCAAG 
GCCAATTCAT TCGATGGCTT TGCTCAAAAT GTACACGGGG ACTCGCTTAA CATCTATCCA 
ACTAACTCTG TGTCGTCATC TGGACTCTGC AAGTCCGATT CCTGCTTGTA TAAACGCGGC 
CAAACAGACC AAATCACAGA CATGCTGATT CATGAAACCT GGTCCAGCTC TATCGAATCT 
CTAATGCGCA AGAATAAGAT AATTGCCGAG CCCTCAGAGG ACAGTCTGGA ACTCGATTCT 
GCAGACTCAC AGCCACATGT ACAGCAGTTT GCTAATCGTC TAGCGGCAGA CATCGTAGAG 
AGTGGCAAAT CACAGCTTGG AGGCCAGCAG GATGTCACTG CGGCTGCTTG TCAGCCACAT 
ATTCCAGTTG GAGAAAGGAG ACACGGCTTT AAACATTCCC GTTGCAACAA CAGTGGAAAC 
CGGCCAGGCG TGGAGCATCA GGAAAACAGC TGTAGCTCTT ACAGTTCCTC CTGTGTGAGG 
CAGGCATGGC GCGGACAGAG AGAGGTACCC TTGATTCACA TTGAACCAGA TCAAAGAGAA 
GAGGCCTCGG AAGAGAAGGG AGGGGTTGAG ACCCACCACA GGGAGGCATC CCATCAGACC 
CAGCAACAAA GTGGCAAAGG GAGTGAGACA GCAACCAAAA GCAGCAGTGA CTCAGACAGG 
GTCTCCTCAG TGGCCACAGT GCCCCCTGCT GGTGTGGAGG TGGAGCGGCG GTCTCTCAGC 
GCTAGCAGTG AGGAAAGTGG CTCAGGCAGC TGGGCACAGA TTGCCACTGA AGACGACCCC 
CAGGAGGAGA CCACCAGTAG CTTTATCCAG CTAAGCGAGG GAAATGGGAA CAGCAGCACT 
TCTAGTCTGG GACTGGCCGA CCTAGAGGGC TTTCCGGACT TCTCCATCTC CAGCAACCTA 
ATCAGTGAGG AGAGGGAGAG GAAGACTTCG CACTGTCAGG ACCAGGCGGA CGAGGTGACG 
TCGGGCTTGT CGACGGCCGG CAGCAGCTGT CAGCGGGAGC TCCTGGTGCT GAACTGTGAC 
CTGGAGCCGG ACTGTGTGGA CGGAGAGCTG AGAGCCGCGC TGCAGTGGAT CTCAGCATCT 
GAACTCGGCG TCCCGGCGCT TTACTTCAGA AAGACTCACC AGCACAATCT TACAAAGCTC 
CACAGAGTGC TGCAGTTGGC AGGTCAGAAG GCTTGGCGTG TTGGAGACTT GTTCAGCGCC 
GTGGCTCAGT TCTGTCAGCT CCACCAGGAC CTGGAGCTGA AAGGACGGCC GCTGCCCAGC 
CTGTTTGACT GGATCCTGAA GACCAAACGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sphkap ( XM_009293857.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292132.1 Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X2, mRNA.

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CloneID	ODa48352
Clone ID Related Accession (Same CDS sequence)	XM_009293857.2 , XM_009293858.2 , XM_009293857.3
Accession Version	XM_009293857.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAGG GATCTGAGTC CGCGAACACA CAGGAAGTCA AATCTGAGAG CACCCTTGGG 
TCCTCGCTCT CCGCCTGCAA GAAGGTTTTG TGCAGTAACA GTGTTCTGGA TTCGTCTGAG 
TACTGGCTGA AGAATGATAA GACGCTCTGC AGAATTGGAT TTCTGGAGGA CCAGCATGAC 
AGCGGCTGCC CGACAATTTG CTTTGTAAAC CTGGACAGAC ATACTTCAGA TTGGCATGAT 
GACAACTACA TCAAGAAGCT GGCCTCGGTG TGCCCAGAGC TGCCACGGTT AATTGAGTCT 
CTCGGAGTGC GGCAACCCAA AGAAAATGAG ATTCTGCTTC TCAGCAGCCT AGAGCCACCG 
GACTCCTGCC AGCATGACCC CAGCCACCCA CAGAGCCCGA GAACAGCGGA TGTCTGTCTG 
GTGCATTGTT CATGTGGACG CCGTCCCACC CAAACGAGCA GTATCATTTT TGAGATTAAC 
AAGTTCCTGA TTGGACTACA GTCAGGGCAG GAGAGGCAGA AGCATGGCCG ATTGGCTGGA 
CAACGAGCTG CTGAAGATGA CACCAATCGC TCTGTTTCGT CGATTGAAGA AGATTTCCTC 
ACTGCCTCAG AGCAGCTTGG GGAAGACAGC GAGGAAGACC CTTTCAGGAA TGATCCTGAG 
AATTCTCTCA TGCCCGAGTC TGTTAAGGTG TTGAGAGCAG CCCACGCTCA AAAAAGGAGT 
GAAATAGAGA GAGAGGACAG TGAGGACTCT GAAACCCTCT GCACCTCTTC CAACTCCCAA 
AAGCTTTCAA GAACATATTC CGCCCCTGCA AGAGGCCGAC CCACCACCCC AAAACAAGTC 
AAGGAGTCAG CCGGTCACTA CGCCACCAAT CTGGCTGAGT CAGTCCTGCA AGATGCTTTT 
ATTCGTTTGT CACAAGATGA GCCATCATTT GTTGCAGAAG CAGCAGTGAG TGTGTCAAGT 
GATATAAGCC ATTCCACTGA GGCACCACAG AGGGCTCGAG CTTGCTCATT CGAACTTCCT 
AAAATTGTGA TTGTCCAAAG TCCTGATAAC AGTGAGGAGG TAACAGAATG GCCGGAAGTG 
CAGTCACATG CAACATCAGA GCATGATAGT GGAAATAAGG CCAAAGCAAA GCATGGTACT 
CATCCCCCGC ACAACTCGCC AGTTGGACAC CCTGCTAAGC CATTGGAAAT AGCTTTGGCA 
TGTGCTGCAA GTGTCATTGG TACCATTACC ACCCCTCAAG TCACAGAACA ACTCACACTA 
GAGTCAGGAG AGGAATATGA GTGTGAAGAT GAAGAGGAAG AAAGTGAAAC TGATCAAGGG 
GAGTATTCGT TTTCATCTGC GGTATGTGGC ATGTCTCAGG TCGCTGGAGC TGTAGCTGCG 
GTCGATCTAG CAGATGACTC TGGGGACAAT GAAGACTTGG CAGACATGTA TTCCGCCTCT 
ATGGGACTTC TGTCAGTTGC CCAAGCTTCA ACTGCAATAC CTCTTCACTG TAGCATAGCA 
GAGGGCACCA GCGTTGAGGC CTTTCGAGCC AATGTGGCTG AAGTTCTCCT CAGGGAAGCT 
TCAGCTGTAT TCACTCACAG ACAAAGTTAT TCAAGTGTTG CAAATTTCCT TGAGACCACA 
CATAACAAGA TAGTGGATGG CATCACAAAT CCAAGAAGAC CGTATCAGGA GCAACAAGAT 
GTGGATAACT TCACTCAAGA AATATCTGAC AGTATCTTTC AGTATGCCCT TGAGAAGGCG 
GAGAAGAGAA AAGAACTTGA GGGTCCTGGA AAAGATGCCC CTAACATTGA GGGTTTCCTC 
TCGGATTGTG TGAATAATTT GCTCTTTGAT GTACTGTGTG TAACATCAAG GAAAATAAGT 
GACATTTCAA ATTGTAATAT GGAATCATGT GATGGGCAGG AAGGCAGTGT CGGCTATAGG 
GCATATGAGG CTGATTCCAA AGCTGGCAGG GAAGCATTGA GCCAATTGCA GCATTTGATT 
GAGCCAAGCC AGGCTTATAA TCATGGTGAA AGGCGAAGCA GTGTGGAGAA GTTATCTGCT 
CTAATAGGAA AGAGAGAGAG AGAGCAGAAA CACATACAGG AGGAAAGAGA AAATGAAGCC 
AGACTGAAGG AACCCTACAG ACTTGCTCTG AACAGAGTGA CAGCCACTAT GGTCAAAGAG 
CAGTCTGACC TGCAAGGGCA GCTGGGCAGG AGTGACAAGA ATGCAGACTC TGCGCTCCCT 
TCAGCAGAAA GCTCACCACT TCACATGCAA CGAGGGAGGG CAGGAGGAGA GAGCGAGACC 
AGGCAGCAGT CTTTATCGTC CTCTTCTGGA GCACTTGTGG CACTTAAAAT GGATTCGGGG 
GAGTCCAAAA CTCCTGTCAC GTGCTTTGCT GAAGATTTGG CGACCACAGT GGTCTCCATG 
GCTACAGAGC TGGCAGCGAT ATGCTTGGAG AACTCAAGTG GAAAGCAGCC GTGGTTCTGC 
GCATTAAATG GTGGCTCGGA AGGTCCAGAA GGGCTGCTTC TGCCCTGCCG CACAGCTGCA 
GCTCTCCGCC GAAAGGAGAC ACAAAATGGC ACCTCGGTCA CCAAGAAGCA CAGACCGCCA 
CGTCTCAGTG AAATCAAACG TAAAACCGAA GAACAGCCCG AACTCATGGA GCGCCTGGTC 
AACCGTGTTG TAGATGAAAC CGTTAACTTA GATGAACCCA CAACCACTGA TCCATTCGCG 
CTCTTTGCAT CAGAGGTCAC TGCCAGGATA ATGAACTGCC CAGAATTAAG TGTAGTGGAC 
ACTTCCAAAT CTGGGCAGTC GTCCCGCAGC CGCCTGCAGT GTGAGAGATG GAGTAGCAGG 
GGGAAGGCAT CAAGCTATGA GAGCATTCCA GAGGAGGATG CAGATCCCTC CGGGACATCA 
AATACTTTGG GTCCAGGAAA CAGGCTTGGG CATAATTTGA GCCGAGGAAG CTCTATCTCC 
AAGCAGTCCA GCTGTGAGAG CATTACGGAT GAGTTCTCTC GCTTCATGGT GAACCAGATG 
GAGACAGAGG GTCGAGGGTT TGACCTTCTG CTGGACTACT ATGCTGGGAA AAATGCCAGC 
AGCATCCTTG CAGCAGCTGT TCAGCAGGCT GCCAGTAAAA AAAACGGACA CCTCAATGTG 
CGTACATCAT CTTGTCTCTC GAAGCAGTCC AGCACTGAGA GTATCACAGA AGAGTTTTAT 
CGCTTCATGC TGAAAGATAT GGACAAGGAA AACAAGGACT ACAGTATGAC CAAGACCAAA 
GAGTGGAGTA ATAGCCTGCT ACCCCCGTCC CCCAGAACCC CTTTTTGTAT CCGTCAGTCT 
TCTGTTCCTG ACAGGCGGTC TTCAGATTCC AGACTAACTG TAAACTCGCC TATCAAGGCC 
AATTCATTCG ATGGCTTTGC TCAAAATGTA CACGGGGACT CGCTTAACAT CTATCCAACT 
AACTCTGTGT CGTCATCTGG ACTCTGCAAG TCCGATTCCT GCTTGTATAA ACGCGGCCAA 
ACAGACCAAA TCACAGACAT GCTGATTCAT GAAACCTGGT CCAGCTCTAT CGAATCTCTA 
ATGCGCAAGA ATAAGATAAT TGCCGAGCCC TCAGAGGACA GTCTGGAACT CGATTCTGCA 
GACTCACAGC CACATGTACA GCAGTTTGCT AATCGTCTAG CGGCAGACAT CGTAGAGAGT 
GGCAAATCAC AGCTTGGAGG CCAGCAGGAT GTCACTGCGG CTGCTTGTCA GCCACATATT 
CCAGTTGGAG AAAGGAGACA CGGCTTTAAA CATTCCCGTT GCAACAACAG TGGAAACCGG 
CCAGGCGTGG AGCATCAGGA AAACAGCTGT AGCTCTTACA GTTCCTCCTG TGTGAGGCAG 
GCATGGCGCG GACAGAGAGA GGTACCCTTG ATTCACATTG AACCAGATCA AAGAGAAGAG 
GCCTCGGAAG AGAAGGGAGG GGTTGAGACC CACCACAGGG AGGCATCCCA TCAGACCCAG 
CAACAAAGTG GCAAAGGGAG TGAGACAGCA ACCAAAAGCA GCAGTGACTC AGACAGGGTC 
TCCTCAGTGG CCACAGTGCC CCCTGCTGGT GTGGAGGTGG AGCGGCGGTC TCTCAGCGCT 
AGCAGTGAGG AAAGTGGCTC AGGCAGCTGG GCACAGATTG CCACTGAAGA CGACCCCCAG 
GAGGAGACCA CCAGTAGCTT TATCCAGCTA AGCGAGGGAA ATGGGAACAG CAGCACTTCT 
AGTCTGGGAC TGGCCGACCT AGAGGGCTTT CCGGACTTCT CCATCTCCAG CAACCTAATC 
AGTGAGGAGA GGGAGAGGAA GACTTCGCAC TGTCAGGACC AGGCGGACGA GGTGACGTCG 
GGCTTGTCGA CGGCCGGCAG CAGCTGTCAG CGGGAGCTCC TGGTGCTGAA CTGTGACCTG 
GAGCCGGACT GTGTGGACGG AGAGCTGAGA GCCGCGCTGC AGTGGATCTC AGCATCTGAA 
CTCGGCGTCC CGGCGCTTTA CTTCAGAAAG ACTCACCAGC ACAATCTTAC AAAGCTCCAC 
AGAGTGCTGC AGTTGGCAGG TCAGAAGGCT TGGCGTGTTG GAGACTTGTT CAGCGCCGTG 
GCTCAGTTCT GTCAGCTCCA CCAGGACCTG GAGCTGAAAG GACGGCCGCT GCCCAGCCTG 
TTTGACTGGA TCCTGAAGAC CAAACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292132.1
CDS	362..5011
Translation	MFEGSESANTQEVKSESTLGSSLSACKKVLCSNSVLDSSEYWLKNDKTLCRIGFLEDQHDSGCPTICFVNLDRHTSDWHDDNYIKKLASVCPELPRLIESLGVRQPKENEILLLSSLEPPDSCQHDPSHPQSPRTADVCLVHCSCGRRPTQTSSIIFEINKFLIGLQSGQERQKHGRLAGQRAAEDDTNRSVSSIEEDFLTASEQLGEDSEEDPFRNDPENSLMPESVKVLRAAHAQKRSEIEREDSEDSETLCTSSNSQKLSRTYSAPARGRPTTPKQVKESAGHYATNLAESVLQDAFIRLSQDEPSFVAEAAVSVSSDISHSTEAPQRARACSFELPKIVIVQSPDNSEEVTEWPEVQSHATSEHDSGNKAKAKHGTHPPHNSPVGHPAKPLEIALACAASVIGTITTPQVTEQLTLESGEEYECEDEEEESETDQGEYSFSSAVCGMSQVAGAVAAVDLADDSGDNEDLADMYSASMGLLSVAQASTAIPLHCSIAEGTSVEAFRANVAEVLLREASAVFTHRQSYSSVANFLETTHNKIVDGITNPRRPYQEQQDVDNFTQEISDSIFQYALEKAEKRKELEGPGKDAPNIEGFLSDCVNNLLFDVLCVTSRKISDISNCNMESCDGQEGSVGYRAYEADSKAGREALSQLQHLIEPSQAYNHGERRSSVEKLSALIGKREREQKHIQEERENEARLKEPYRLALNRVTATMVKEQSDLQGQLGRSDKNADSALPSAESSPLHMQRGRAGGESETRQQSLSSSSGALVALKMDSGESKTPVTCFAEDLATTVVSMATELAAICLENSSGKQPWFCALNGGSEGPEGLLLPCRTAAALRRKETQNGTSVTKKHRPPRLSEIKRKTEEQPELMERLVNRVVDETVNLDEPTTTDPFALFASEVTARIMNCPELSVVDTSKSGQSSRSRLQCERWSSRGKASSYESIPEEDADPSGTSNTLGPGNRLGHNLSRGSSISKQSSCESITDEFSRFMVNQMETEGRGFDLLLDYYAGKNASSILAAAVQQAASKKNGHLNVRTSSCLSKQSSTESITEEFYRFMLKDMDKENKDYSMTKTKEWSNSLLPPSPRTPFCIRQSSVPDRRSSDSRLTVNSPIKANSFDGFAQNVHGDSLNIYPTNSVSSSGLCKSDSCLYKRGQTDQITDMLIHETWSSSIESLMRKNKIIAEPSEDSLELDSADSQPHVQQFANRLAADIVESGKSQLGGQQDVTAAACQPHIPVGERRHGFKHSRCNNSGNRPGVEHQENSCSSYSSSCVRQAWRGQREVPLIHIEPDQREEASEEKGGVETHHREASHQTQQQSGKGSETATKSSSDSDRVSSVATVPPAGVEVERRSLSASSEESGSGSWAQIATEDDPQEETTSSFIQLSEGNGNSSTSSLGLADLEGFPDFSISSNLISEERERKTSHCQDQADEVTSGLSTAGSSCQRELLVLNCDLEPDCVDGELRAALQWISASELGVPALYFRKTHQHNLTKLHRVLQLAGQKAWRVGDLFSAVAQFCQLHQDLELKGRPLPSLFDWILKTKR

Target ORF information:

RefSeq Version	XM_009293857.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293857.2

ORF Insert Sequence:

ATGTTTGAGG GATCTGAGTC CGCGAACACA CAGGAAGTCA AATCTGAGAG CACCCTTGGG 
TCCTCGCTCT CCGCCTGCAA GAAGGTTTTG TGCAGTAACA GTGTTCTGGA TTCGTCTGAG 
TACTGGCTGA AGAATGATAA GACGCTCTGC AGAATTGGAT TTCTGGAGGA CCAGCATGAC 
AGCGGCTGCC CGACAATTTG CTTTGTAAAC CTGGACAGAC ATACTTCAGA TTGGCATGAT 
GACAACTACA TCAAGAAGCT GGCCTCGGTG TGCCCAGAGC TGCCACGGTT AATTGAGTCT 
CTCGGAGTGC GGCAACCCAA AGAAAATGAG ATTCTGCTTC TCAGCAGCCT AGAGCCACCG 
GACTCCTGCC AGCATGACCC CAGCCACCCA CAGAGCCCGA GAACAGCGGA TGTCTGTCTG 
GTGCATTGTT CATGTGGACG CCGTCCCACC CAAACGAGCA GTATCATTTT TGAGATTAAC 
AAGTTCCTGA TTGGACTACA GTCAGGGCAG GAGAGGCAGA AGCATGGCCG ATTGGCTGGA 
CAACGAGCTG CTGAAGATGA CACCAATCGC TCTGTTTCGT CGATTGAAGA AGATTTCCTC 
ACTGCCTCAG AGCAGCTTGG GGAAGACAGC GAGGAAGACC CTTTCAGGAA TGATCCTGAG 
AATTCTCTCA TGCCCGAGTC TGTTAAGGTG TTGAGAGCAG CCCACGCTCA AAAAAGGAGT 
GAAATAGAGA GAGAGGACAG TGAGGACTCT GAAACCCTCT GCACCTCTTC CAACTCCCAA 
AAGCTTTCAA GAACATATTC CGCCCCTGCA AGAGGCCGAC CCACCACCCC AAAACAAGTC 
AAGGAGTCAG CCGGTCACTA CGCCACCAAT CTGGCTGAGT CAGTCCTGCA AGATGCTTTT 
ATTCGTTTGT CACAAGATGA GCCATCATTT GTTGCAGAAG CAGCAGTGAG TGTGTCAAGT 
GATATAAGCC ATTCCACTGA GGCACCACAG AGGGCTCGAG CTTGCTCATT CGAACTTCCT 
AAAATTGTGA TTGTCCAAAG TCCTGATAAC AGTGAGGAGG TAACAGAATG GCCGGAAGTG 
CAGTCACATG CAACATCAGA GCATGATAGT GGAAATAAGG CCAAAGCAAA GCATGGTACT 
CATCCCCCGC ACAACTCGCC AGTTGGACAC CCTGCTAAGC CATTGGAAAT AGCTTTGGCA 
TGTGCTGCAA GTGTCATTGG TACCATTACC ACCCCTCAAG TCACAGAACA ACTCACACTA 
GAGTCAGGAG AGGAATATGA GTGTGAAGAT GAAGAGGAAG AAAGTGAAAC TGATCAAGGG 
GAGTATTCGT TTTCATCTGC GGTATGTGGC ATGTCTCAGG TCGCTGGAGC TGTAGCTGCG 
GTCGATCTAG CAGATGACTC TGGGGACAAT GAAGACTTGG CAGACATGTA TTCCGCCTCT 
ATGGGACTTC TGTCAGTTGC CCAAGCTTCA ACTGCAATAC CTCTTCACTG TAGCATAGCA 
GAGGGCACCA GCGTTGAGGC CTTTCGAGCC AATGTGGCTG AAGTTCTCCT CAGGGAAGCT 
TCAGCTGTAT TCACTCACAG ACAAAGTTAT TCAAGTGTTG CAAATTTCCT TGAGACCACA 
CATAACAAGA TAGTGGATGG CATCACAAAT CCAAGAAGAC CGTATCAGGA GCAACAAGAT 
GTGGATAACT TCACTCAAGA AATATCTGAC AGTATCTTTC AGTATGCCCT TGAGAAGGCG 
GAGAAGAGAA AAGAACTTGA GGGTCCTGGA AAAGATGCCC CTAACATTGA GGGTTTCCTC 
TCGGATTGTG TGAATAATTT GCTCTTTGAT GTACTGTGTG TAACATCAAG GAAAATAAGT 
GACATTTCAA ATTGTAATAT GGAATCATGT GATGGGCAGG AAGGCAGTGT CGGCTATAGG 
GCATATGAGG CTGATTCCAA AGCTGGCAGG GAAGCATTGA GCCAATTGCA GCATTTGATT 
GAGCCAAGCC AGGCTTATAA TCATGGTGAA AGGCGAAGCA GTGTGGAGAA GTTATCTGCT 
CTAATAGGAA AGAGAGAGAG AGAGCAGAAA CACATACAGG AGGAAAGAGA AAATGAAGCC 
AGACTGAAGG AACCCTACAG ACTTGCTCTG AACAGAGTGA CAGCCACTAT GGTCAAAGAG 
CAGTCTGACC TGCAAGGGCA GCTGGGCAGG AGTGACAAGA ATGCAGACTC TGCGCTCCCT 
TCAGCAGAAA GCTCACCACT TCACATGCAA CGAGGGAGGG CAGGAGGAGA GAGCGAGACC 
AGGCAGCAGT CTTTATCGTC CTCTTCTGGA GCACTTGTGG CACTTAAAAT GGATTCGGGG 
GAGTCCAAAA CTCCTGTCAC GTGCTTTGCT GAAGATTTGG CGACCACAGT GGTCTCCATG 
GCTACAGAGC TGGCAGCGAT ATGCTTGGAG AACTCAAGTG GAAAGCAGCC GTGGTTCTGC 
GCATTAAATG GTGGCTCGGA AGGTCCAGAA GGGCTGCTTC TGCCCTGCCG CACAGCTGCA 
GCTCTCCGCC GAAAGGAGAC ACAAAATGGC ACCTCGGTCA CCAAGAAGCA CAGACCGCCA 
CGTCTCAGTG AAATCAAACG TAAAACCGAA GAACAGCCCG AACTCATGGA GCGCCTGGTC 
AACCGTGTTG TAGATGAAAC CGTTAACTTA GATGAACCCA CAACCACTGA TCCATTCGCG 
CTCTTTGCAT CAGAGGTCAC TGCCAGGATA ATGAACTGCC CAGAATTAAG TGTAGTGGAC 
ACTTCCAAAT CTGGGCAGTC GTCCCGCAGC CGCCTGCAGT GTGAGAGATG GAGTAGCAGG 
GGGAAGGCAT CAAGCTATGA GAGCATTCCA GAGGAGGATG CAGATCCCTC CGGGACATCA 
AATACTTTGG GTCCAGGAAA CAGGCTTGGG CATAATTTGA GCCGAGGAAG CTCTATCTCC 
AAGCAGTCCA GCTGTGAGAG CATTACGGAT GAGTTCTCTC GCTTCATGGT GAACCAGATG 
GAGACAGAGG GTCGAGGGTT TGACCTTCTG CTGGACTACT ATGCTGGGAA AAATGCCAGC 
AGCATCCTTG CAGCAGCTGT TCAGCAGGCT GCCAGTAAAA AAAACGGACA CCTCAATGTG 
CGTACATCAT CTTGTCTCTC GAAGCAGTCC AGCACTGAGA GTATCACAGA AGAGTTTTAT 
CGCTTCATGC TGAAAGATAT GGACAAGGAA AACAAGGACT ACAGTATGAC CAAGACCAAA 
GAGTGGAGTA ATAGCCTGCT ACCCCCGTCC CCCAGAACCC CTTTTTGTAT CCGTCAGTCT 
TCTGTTCCTG ACAGGCGGTC TTCAGATTCC AGACTAACTG TAAACTCGCC TATCAAGGCC 
AATTCATTCG ATGGCTTTGC TCAAAATGTA CACGGGGACT CGCTTAACAT CTATCCAACT 
AACTCTGTGT CGTCATCTGG ACTCTGCAAG TCCGATTCCT GCTTGTATAA ACGCGGCCAA 
ACAGACCAAA TCACAGACAT GCTGATTCAT GAAACCTGGT CCAGCTCTAT CGAATCTCTA 
ATGCGCAAGA ATAAGATAAT TGCCGAGCCC TCAGAGGACA GTCTGGAACT CGATTCTGCA 
GACTCACAGC CACATGTACA GCAGTTTGCT AATCGTCTAG CGGCAGACAT CGTAGAGAGT 
GGCAAATCAC AGCTTGGAGG CCAGCAGGAT GTCACTGCGG CTGCTTGTCA GCCACATATT 
CCAGTTGGAG AAAGGAGACA CGGCTTTAAA CATTCCCGTT GCAACAACAG TGGAAACCGG 
CCAGGCGTGG AGCATCAGGA AAACAGCTGT AGCTCTTACA GTTCCTCCTG TGTGAGGCAG 
GCATGGCGCG GACAGAGAGA GGTACCCTTG ATTCACATTG AACCAGATCA AAGAGAAGAG 
GCCTCGGAAG AGAAGGGAGG GGTTGAGACC CACCACAGGG AGGCATCCCA TCAGACCCAG 
CAACAAAGTG GCAAAGGGAG TGAGACAGCA ACCAAAAGCA GCAGTGACTC AGACAGGGTC 
TCCTCAGTGG CCACAGTGCC CCCTGCTGGT GTGGAGGTGG AGCGGCGGTC TCTCAGCGCT 
AGCAGTGAGG AAAGTGGCTC AGGCAGCTGG GCACAGATTG CCACTGAAGA CGACCCCCAG 
GAGGAGACCA CCAGTAGCTT TATCCAGCTA AGCGAGGGAA ATGGGAACAG CAGCACTTCT 
AGTCTGGGAC TGGCCGACCT AGAGGGCTTT CCGGACTTCT CCATCTCCAG CAACCTAATC 
AGTGAGGAGA GGGAGAGGAA GACTTCGCAC TGTCAGGACC AGGCGGACGA GGTGACGTCG 
GGCTTGTCGA CGGCCGGCAG CAGCTGTCAG CGGGAGCTCC TGGTGCTGAA CTGTGACCTG 
GAGCCGGACT GTGTGGACGG AGAGCTGAGA GCCGCGCTGC AGTGGATCTC AGCATCTGAA 
CTCGGCGTCC CGGCGCTTTA CTTCAGAAAG ACTCACCAGC ACAATCTTAC AAAGCTCCAC 
AGAGTGCTGC AGTTGGCAGG TCAGAAGGCT TGGCGTGTTG GAGACTTGTT CAGCGCCGTG 
GCTCAGTTCT GTCAGCTCCA CCAGGACCTG GAGCTGAAAG GACGGCCGCT GCCCAGCCTG 
TTTGACTGGA TCCTGAAGAC CAAACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sphkap ( XM_009293858.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292133.1 Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X3, mRNA.

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CloneID	ODa48352
Clone ID Related Accession (Same CDS sequence)	XM_009293857.2 , XM_009293858.2 , XM_009293857.3
Accession Version	XM_009293858.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293858.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAGG GATCTGAGTC CGCGAACACA CAGGAAGTCA AATCTGAGAG CACCCTTGGG 
TCCTCGCTCT CCGCCTGCAA GAAGGTTTTG TGCAGTAACA GTGTTCTGGA TTCGTCTGAG 
TACTGGCTGA AGAATGATAA GACGCTCTGC AGAATTGGAT TTCTGGAGGA CCAGCATGAC 
AGCGGCTGCC CGACAATTTG CTTTGTAAAC CTGGACAGAC ATACTTCAGA TTGGCATGAT 
GACAACTACA TCAAGAAGCT GGCCTCGGTG TGCCCAGAGC TGCCACGGTT AATTGAGTCT 
CTCGGAGTGC GGCAACCCAA AGAAAATGAG ATTCTGCTTC TCAGCAGCCT AGAGCCACCG 
GACTCCTGCC AGCATGACCC CAGCCACCCA CAGAGCCCGA GAACAGCGGA TGTCTGTCTG 
GTGCATTGTT CATGTGGACG CCGTCCCACC CAAACGAGCA GTATCATTTT TGAGATTAAC 
AAGTTCCTGA TTGGACTACA GTCAGGGCAG GAGAGGCAGA AGCATGGCCG ATTGGCTGGA 
CAACGAGCTG CTGAAGATGA CACCAATCGC TCTGTTTCGT CGATTGAAGA AGATTTCCTC 
ACTGCCTCAG AGCAGCTTGG GGAAGACAGC GAGGAAGACC CTTTCAGGAA TGATCCTGAG 
AATTCTCTCA TGCCCGAGTC TGTTAAGGTG TTGAGAGCAG CCCACGCTCA AAAAAGGAGT 
GAAATAGAGA GAGAGGACAG TGAGGACTCT GAAACCCTCT GCACCTCTTC CAACTCCCAA 
AAGCTTTCAA GAACATATTC CGCCCCTGCA AGAGGCCGAC CCACCACCCC AAAACAAGTC 
AAGGAGTCAG CCGGTCACTA CGCCACCAAT CTGGCTGAGT CAGTCCTGCA AGATGCTTTT 
ATTCGTTTGT CACAAGATGA GCCATCATTT GTTGCAGAAG CAGCAGTGAG TGTGTCAAGT 
GATATAAGCC ATTCCACTGA GGCACCACAG AGGGCTCGAG CTTGCTCATT CGAACTTCCT 
AAAATTGTGA TTGTCCAAAG TCCTGATAAC AGTGAGGAGG TAACAGAATG GCCGGAAGTG 
CAGTCACATG CAACATCAGA GCATGATAGT GGAAATAAGG CCAAAGCAAA GCATGGTACT 
CATCCCCCGC ACAACTCGCC AGTTGGACAC CCTGCTAAGC CATTGGAAAT AGCTTTGGCA 
TGTGCTGCAA GTGTCATTGG TACCATTACC ACCCCTCAAG TCACAGAACA ACTCACACTA 
GAGTCAGGAG AGGAATATGA GTGTGAAGAT GAAGAGGAAG AAAGTGAAAC TGATCAAGGG 
GAGTATTCGT TTTCATCTGC GGTATGTGGC ATGTCTCAGG TCGCTGGAGC TGTAGCTGCG 
GTCGATCTAG CAGATGACTC TGGGGACAAT GAAGACTTGG CAGACATGTA TTCCGCCTCT 
ATGGGACTTC TGTCAGTTGC CCAAGCTTCA ACTGCAATAC CTCTTCACTG TAGCATAGCA 
GAGGGCACCA GCGTTGAGGC CTTTCGAGCC AATGTGGCTG AAGTTCTCCT CAGGGAAGCT 
TCAGCTGTAT TCACTCACAG ACAAAGTTAT TCAAGTGTTG CAAATTTCCT TGAGACCACA 
CATAACAAGA TAGTGGATGG CATCACAAAT CCAAGAAGAC CGTATCAGGA GCAACAAGAT 
GTGGATAACT TCACTCAAGA AATATCTGAC AGTATCTTTC AGTATGCCCT TGAGAAGGCG 
GAGAAGAGAA AAGAACTTGA GGGTCCTGGA AAAGATGCCC CTAACATTGA GGGTTTCCTC 
TCGGATTGTG TGAATAATTT GCTCTTTGAT GTACTGTGTG TAACATCAAG GAAAATAAGT 
GACATTTCAA ATTGTAATAT GGAATCATGT GATGGGCAGG AAGGCAGTGT CGGCTATAGG 
GCATATGAGG CTGATTCCAA AGCTGGCAGG GAAGCATTGA GCCAATTGCA GCATTTGATT 
GAGCCAAGCC AGGCTTATAA TCATGGTGAA AGGCGAAGCA GTGTGGAGAA GTTATCTGCT 
CTAATAGGAA AGAGAGAGAG AGAGCAGAAA CACATACAGG AGGAAAGAGA AAATGAAGCC 
AGACTGAAGG AACCCTACAG ACTTGCTCTG AACAGAGTGA CAGCCACTAT GGTCAAAGAG 
CAGTCTGACC TGCAAGGGCA GCTGGGCAGG AGTGACAAGA ATGCAGACTC TGCGCTCCCT 
TCAGCAGAAA GCTCACCACT TCACATGCAA CGAGGGAGGG CAGGAGGAGA GAGCGAGACC 
AGGCAGCAGT CTTTATCGTC CTCTTCTGGA GCACTTGTGG CACTTAAAAT GGATTCGGGG 
GAGTCCAAAA CTCCTGTCAC GTGCTTTGCT GAAGATTTGG CGACCACAGT GGTCTCCATG 
GCTACAGAGC TGGCAGCGAT ATGCTTGGAG AACTCAAGTG GAAAGCAGCC GTGGTTCTGC 
GCATTAAATG GTGGCTCGGA AGGTCCAGAA GGGCTGCTTC TGCCCTGCCG CACAGCTGCA 
GCTCTCCGCC GAAAGGAGAC ACAAAATGGC ACCTCGGTCA CCAAGAAGCA CAGACCGCCA 
CGTCTCAGTG AAATCAAACG TAAAACCGAA GAACAGCCCG AACTCATGGA GCGCCTGGTC 
AACCGTGTTG TAGATGAAAC CGTTAACTTA GATGAACCCA CAACCACTGA TCCATTCGCG 
CTCTTTGCAT CAGAGGTCAC TGCCAGGATA ATGAACTGCC CAGAATTAAG TGTAGTGGAC 
ACTTCCAAAT CTGGGCAGTC GTCCCGCAGC CGCCTGCAGT GTGAGAGATG GAGTAGCAGG 
GGGAAGGCAT CAAGCTATGA GAGCATTCCA GAGGAGGATG CAGATCCCTC CGGGACATCA 
AATACTTTGG GTCCAGGAAA CAGGCTTGGG CATAATTTGA GCCGAGGAAG CTCTATCTCC 
AAGCAGTCCA GCTGTGAGAG CATTACGGAT GAGTTCTCTC GCTTCATGGT GAACCAGATG 
GAGACAGAGG GTCGAGGGTT TGACCTTCTG CTGGACTACT ATGCTGGGAA AAATGCCAGC 
AGCATCCTTG CAGCAGCTGT TCAGCAGGCT GCCAGTAAAA AAAACGGACA CCTCAATGTG 
CGTACATCAT CTTGTCTCTC GAAGCAGTCC AGCACTGAGA GTATCACAGA AGAGTTTTAT 
CGCTTCATGC TGAAAGATAT GGACAAGGAA AACAAGGACT ACAGTATGAC CAAGACCAAA 
GAGTGGAGTA ATAGCCTGCT ACCCCCGTCC CCCAGAACCC CTTTTTGTAT CCGTCAGTCT 
TCTGTTCCTG ACAGGCGGTC TTCAGATTCC AGACTAACTG TAAACTCGCC TATCAAGGCC 
AATTCATTCG ATGGCTTTGC TCAAAATGTA CACGGGGACT CGCTTAACAT CTATCCAACT 
AACTCTGTGT CGTCATCTGG ACTCTGCAAG TCCGATTCCT GCTTGTATAA ACGCGGCCAA 
ACAGACCAAA TCACAGACAT GCTGATTCAT GAAACCTGGT CCAGCTCTAT CGAATCTCTA 
ATGCGCAAGA ATAAGATAAT TGCCGAGCCC TCAGAGGACA GTCTGGAACT CGATTCTGCA 
GACTCACAGC CACATGTACA GCAGTTTGCT AATCGTCTAG CGGCAGACAT CGTAGAGAGT 
GGCAAATCAC AGCTTGGAGG CCAGCAGGAT GTCACTGCGG CTGCTTGTCA GCCACATATT 
CCAGTTGGAG AAAGGAGACA CGGCTTTAAA CATTCCCGTT GCAACAACAG TGGAAACCGG 
CCAGGCGTGG AGCATCAGGA AAACAGCTGT AGCTCTTACA GTTCCTCCTG TGTGAGGCAG 
GCATGGCGCG GACAGAGAGA GGTACCCTTG ATTCACATTG AACCAGATCA AAGAGAAGAG 
GCCTCGGAAG AGAAGGGAGG GGTTGAGACC CACCACAGGG AGGCATCCCA TCAGACCCAG 
CAACAAAGTG GCAAAGGGAG TGAGACAGCA ACCAAAAGCA GCAGTGACTC AGACAGGGTC 
TCCTCAGTGG CCACAGTGCC CCCTGCTGGT GTGGAGGTGG AGCGGCGGTC TCTCAGCGCT 
AGCAGTGAGG AAAGTGGCTC AGGCAGCTGG GCACAGATTG CCACTGAAGA CGACCCCCAG 
GAGGAGACCA CCAGTAGCTT TATCCAGCTA AGCGAGGGAA ATGGGAACAG CAGCACTTCT 
AGTCTGGGAC TGGCCGACCT AGAGGGCTTT CCGGACTTCT CCATCTCCAG CAACCTAATC 
AGTGAGGAGA GGGAGAGGAA GACTTCGCAC TGTCAGGACC AGGCGGACGA GGTGACGTCG 
GGCTTGTCGA CGGCCGGCAG CAGCTGTCAG CGGGAGCTCC TGGTGCTGAA CTGTGACCTG 
GAGCCGGACT GTGTGGACGG AGAGCTGAGA GCCGCGCTGC AGTGGATCTC AGCATCTGAA 
CTCGGCGTCC CGGCGCTTTA CTTCAGAAAG ACTCACCAGC ACAATCTTAC AAAGCTCCAC 
AGAGTGCTGC AGTTGGCAGG TCAGAAGGCT TGGCGTGTTG GAGACTTGTT CAGCGCCGTG 
GCTCAGTTCT GTCAGCTCCA CCAGGACCTG GAGCTGAAAG GACGGCCGCT GCCCAGCCTG 
TTTGACTGGA TCCTGAAGAC CAAACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292133.1
CDS	418..5067
Translation	MFEGSESANTQEVKSESTLGSSLSACKKVLCSNSVLDSSEYWLKNDKTLCRIGFLEDQHDSGCPTICFVNLDRHTSDWHDDNYIKKLASVCPELPRLIESLGVRQPKENEILLLSSLEPPDSCQHDPSHPQSPRTADVCLVHCSCGRRPTQTSSIIFEINKFLIGLQSGQERQKHGRLAGQRAAEDDTNRSVSSIEEDFLTASEQLGEDSEEDPFRNDPENSLMPESVKVLRAAHAQKRSEIEREDSEDSETLCTSSNSQKLSRTYSAPARGRPTTPKQVKESAGHYATNLAESVLQDAFIRLSQDEPSFVAEAAVSVSSDISHSTEAPQRARACSFELPKIVIVQSPDNSEEVTEWPEVQSHATSEHDSGNKAKAKHGTHPPHNSPVGHPAKPLEIALACAASVIGTITTPQVTEQLTLESGEEYECEDEEEESETDQGEYSFSSAVCGMSQVAGAVAAVDLADDSGDNEDLADMYSASMGLLSVAQASTAIPLHCSIAEGTSVEAFRANVAEVLLREASAVFTHRQSYSSVANFLETTHNKIVDGITNPRRPYQEQQDVDNFTQEISDSIFQYALEKAEKRKELEGPGKDAPNIEGFLSDCVNNLLFDVLCVTSRKISDISNCNMESCDGQEGSVGYRAYEADSKAGREALSQLQHLIEPSQAYNHGERRSSVEKLSALIGKREREQKHIQEERENEARLKEPYRLALNRVTATMVKEQSDLQGQLGRSDKNADSALPSAESSPLHMQRGRAGGESETRQQSLSSSSGALVALKMDSGESKTPVTCFAEDLATTVVSMATELAAICLENSSGKQPWFCALNGGSEGPEGLLLPCRTAAALRRKETQNGTSVTKKHRPPRLSEIKRKTEEQPELMERLVNRVVDETVNLDEPTTTDPFALFASEVTARIMNCPELSVVDTSKSGQSSRSRLQCERWSSRGKASSYESIPEEDADPSGTSNTLGPGNRLGHNLSRGSSISKQSSCESITDEFSRFMVNQMETEGRGFDLLLDYYAGKNASSILAAAVQQAASKKNGHLNVRTSSCLSKQSSTESITEEFYRFMLKDMDKENKDYSMTKTKEWSNSLLPPSPRTPFCIRQSSVPDRRSSDSRLTVNSPIKANSFDGFAQNVHGDSLNIYPTNSVSSSGLCKSDSCLYKRGQTDQITDMLIHETWSSSIESLMRKNKIIAEPSEDSLELDSADSQPHVQQFANRLAADIVESGKSQLGGQQDVTAAACQPHIPVGERRHGFKHSRCNNSGNRPGVEHQENSCSSYSSSCVRQAWRGQREVPLIHIEPDQREEASEEKGGVETHHREASHQTQQQSGKGSETATKSSSDSDRVSSVATVPPAGVEVERRSLSASSEESGSGSWAQIATEDDPQEETTSSFIQLSEGNGNSSTSSLGLADLEGFPDFSISSNLISEERERKTSHCQDQADEVTSGLSTAGSSCQRELLVLNCDLEPDCVDGELRAALQWISASELGVPALYFRKTHQHNLTKLHRVLQLAGQKAWRVGDLFSAVAQFCQLHQDLELKGRPLPSLFDWILKTKR

Target ORF information:

RefSeq Version	XM_009293858.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293858.2

ORF Insert Sequence:

ATGTTTGAGG GATCTGAGTC CGCGAACACA CAGGAAGTCA AATCTGAGAG CACCCTTGGG 
TCCTCGCTCT CCGCCTGCAA GAAGGTTTTG TGCAGTAACA GTGTTCTGGA TTCGTCTGAG 
TACTGGCTGA AGAATGATAA GACGCTCTGC AGAATTGGAT TTCTGGAGGA CCAGCATGAC 
AGCGGCTGCC CGACAATTTG CTTTGTAAAC CTGGACAGAC ATACTTCAGA TTGGCATGAT 
GACAACTACA TCAAGAAGCT GGCCTCGGTG TGCCCAGAGC TGCCACGGTT AATTGAGTCT 
CTCGGAGTGC GGCAACCCAA AGAAAATGAG ATTCTGCTTC TCAGCAGCCT AGAGCCACCG 
GACTCCTGCC AGCATGACCC CAGCCACCCA CAGAGCCCGA GAACAGCGGA TGTCTGTCTG 
GTGCATTGTT CATGTGGACG CCGTCCCACC CAAACGAGCA GTATCATTTT TGAGATTAAC 
AAGTTCCTGA TTGGACTACA GTCAGGGCAG GAGAGGCAGA AGCATGGCCG ATTGGCTGGA 
CAACGAGCTG CTGAAGATGA CACCAATCGC TCTGTTTCGT CGATTGAAGA AGATTTCCTC 
ACTGCCTCAG AGCAGCTTGG GGAAGACAGC GAGGAAGACC CTTTCAGGAA TGATCCTGAG 
AATTCTCTCA TGCCCGAGTC TGTTAAGGTG TTGAGAGCAG CCCACGCTCA AAAAAGGAGT 
GAAATAGAGA GAGAGGACAG TGAGGACTCT GAAACCCTCT GCACCTCTTC CAACTCCCAA 
AAGCTTTCAA GAACATATTC CGCCCCTGCA AGAGGCCGAC CCACCACCCC AAAACAAGTC 
AAGGAGTCAG CCGGTCACTA CGCCACCAAT CTGGCTGAGT CAGTCCTGCA AGATGCTTTT 
ATTCGTTTGT CACAAGATGA GCCATCATTT GTTGCAGAAG CAGCAGTGAG TGTGTCAAGT 
GATATAAGCC ATTCCACTGA GGCACCACAG AGGGCTCGAG CTTGCTCATT CGAACTTCCT 
AAAATTGTGA TTGTCCAAAG TCCTGATAAC AGTGAGGAGG TAACAGAATG GCCGGAAGTG 
CAGTCACATG CAACATCAGA GCATGATAGT GGAAATAAGG CCAAAGCAAA GCATGGTACT 
CATCCCCCGC ACAACTCGCC AGTTGGACAC CCTGCTAAGC CATTGGAAAT AGCTTTGGCA 
TGTGCTGCAA GTGTCATTGG TACCATTACC ACCCCTCAAG TCACAGAACA ACTCACACTA 
GAGTCAGGAG AGGAATATGA GTGTGAAGAT GAAGAGGAAG AAAGTGAAAC TGATCAAGGG 
GAGTATTCGT TTTCATCTGC GGTATGTGGC ATGTCTCAGG TCGCTGGAGC TGTAGCTGCG 
GTCGATCTAG CAGATGACTC TGGGGACAAT GAAGACTTGG CAGACATGTA TTCCGCCTCT 
ATGGGACTTC TGTCAGTTGC CCAAGCTTCA ACTGCAATAC CTCTTCACTG TAGCATAGCA 
GAGGGCACCA GCGTTGAGGC CTTTCGAGCC AATGTGGCTG AAGTTCTCCT CAGGGAAGCT 
TCAGCTGTAT TCACTCACAG ACAAAGTTAT TCAAGTGTTG CAAATTTCCT TGAGACCACA 
CATAACAAGA TAGTGGATGG CATCACAAAT CCAAGAAGAC CGTATCAGGA GCAACAAGAT 
GTGGATAACT TCACTCAAGA AATATCTGAC AGTATCTTTC AGTATGCCCT TGAGAAGGCG 
GAGAAGAGAA AAGAACTTGA GGGTCCTGGA AAAGATGCCC CTAACATTGA GGGTTTCCTC 
TCGGATTGTG TGAATAATTT GCTCTTTGAT GTACTGTGTG TAACATCAAG GAAAATAAGT 
GACATTTCAA ATTGTAATAT GGAATCATGT GATGGGCAGG AAGGCAGTGT CGGCTATAGG 
GCATATGAGG CTGATTCCAA AGCTGGCAGG GAAGCATTGA GCCAATTGCA GCATTTGATT 
GAGCCAAGCC AGGCTTATAA TCATGGTGAA AGGCGAAGCA GTGTGGAGAA GTTATCTGCT 
CTAATAGGAA AGAGAGAGAG AGAGCAGAAA CACATACAGG AGGAAAGAGA AAATGAAGCC 
AGACTGAAGG AACCCTACAG ACTTGCTCTG AACAGAGTGA CAGCCACTAT GGTCAAAGAG 
CAGTCTGACC TGCAAGGGCA GCTGGGCAGG AGTGACAAGA ATGCAGACTC TGCGCTCCCT 
TCAGCAGAAA GCTCACCACT TCACATGCAA CGAGGGAGGG CAGGAGGAGA GAGCGAGACC 
AGGCAGCAGT CTTTATCGTC CTCTTCTGGA GCACTTGTGG CACTTAAAAT GGATTCGGGG 
GAGTCCAAAA CTCCTGTCAC GTGCTTTGCT GAAGATTTGG CGACCACAGT GGTCTCCATG 
GCTACAGAGC TGGCAGCGAT ATGCTTGGAG AACTCAAGTG GAAAGCAGCC GTGGTTCTGC 
GCATTAAATG GTGGCTCGGA AGGTCCAGAA GGGCTGCTTC TGCCCTGCCG CACAGCTGCA 
GCTCTCCGCC GAAAGGAGAC ACAAAATGGC ACCTCGGTCA CCAAGAAGCA CAGACCGCCA 
CGTCTCAGTG AAATCAAACG TAAAACCGAA GAACAGCCCG AACTCATGGA GCGCCTGGTC 
AACCGTGTTG TAGATGAAAC CGTTAACTTA GATGAACCCA CAACCACTGA TCCATTCGCG 
CTCTTTGCAT CAGAGGTCAC TGCCAGGATA ATGAACTGCC CAGAATTAAG TGTAGTGGAC 
ACTTCCAAAT CTGGGCAGTC GTCCCGCAGC CGCCTGCAGT GTGAGAGATG GAGTAGCAGG 
GGGAAGGCAT CAAGCTATGA GAGCATTCCA GAGGAGGATG CAGATCCCTC CGGGACATCA 
AATACTTTGG GTCCAGGAAA CAGGCTTGGG CATAATTTGA GCCGAGGAAG CTCTATCTCC 
AAGCAGTCCA GCTGTGAGAG CATTACGGAT GAGTTCTCTC GCTTCATGGT GAACCAGATG 
GAGACAGAGG GTCGAGGGTT TGACCTTCTG CTGGACTACT ATGCTGGGAA AAATGCCAGC 
AGCATCCTTG CAGCAGCTGT TCAGCAGGCT GCCAGTAAAA AAAACGGACA CCTCAATGTG 
CGTACATCAT CTTGTCTCTC GAAGCAGTCC AGCACTGAGA GTATCACAGA AGAGTTTTAT 
CGCTTCATGC TGAAAGATAT GGACAAGGAA AACAAGGACT ACAGTATGAC CAAGACCAAA 
GAGTGGAGTA ATAGCCTGCT ACCCCCGTCC CCCAGAACCC CTTTTTGTAT CCGTCAGTCT 
TCTGTTCCTG ACAGGCGGTC TTCAGATTCC AGACTAACTG TAAACTCGCC TATCAAGGCC 
AATTCATTCG ATGGCTTTGC TCAAAATGTA CACGGGGACT CGCTTAACAT CTATCCAACT 
AACTCTGTGT CGTCATCTGG ACTCTGCAAG TCCGATTCCT GCTTGTATAA ACGCGGCCAA 
ACAGACCAAA TCACAGACAT GCTGATTCAT GAAACCTGGT CCAGCTCTAT CGAATCTCTA 
ATGCGCAAGA ATAAGATAAT TGCCGAGCCC TCAGAGGACA GTCTGGAACT CGATTCTGCA 
GACTCACAGC CACATGTACA GCAGTTTGCT AATCGTCTAG CGGCAGACAT CGTAGAGAGT 
GGCAAATCAC AGCTTGGAGG CCAGCAGGAT GTCACTGCGG CTGCTTGTCA GCCACATATT 
CCAGTTGGAG AAAGGAGACA CGGCTTTAAA CATTCCCGTT GCAACAACAG TGGAAACCGG 
CCAGGCGTGG AGCATCAGGA AAACAGCTGT AGCTCTTACA GTTCCTCCTG TGTGAGGCAG 
GCATGGCGCG GACAGAGAGA GGTACCCTTG ATTCACATTG AACCAGATCA AAGAGAAGAG 
GCCTCGGAAG AGAAGGGAGG GGTTGAGACC CACCACAGGG AGGCATCCCA TCAGACCCAG 
CAACAAAGTG GCAAAGGGAG TGAGACAGCA ACCAAAAGCA GCAGTGACTC AGACAGGGTC 
TCCTCAGTGG CCACAGTGCC CCCTGCTGGT GTGGAGGTGG AGCGGCGGTC TCTCAGCGCT 
AGCAGTGAGG AAAGTGGCTC AGGCAGCTGG GCACAGATTG CCACTGAAGA CGACCCCCAG 
GAGGAGACCA CCAGTAGCTT TATCCAGCTA AGCGAGGGAA ATGGGAACAG CAGCACTTCT 
AGTCTGGGAC TGGCCGACCT AGAGGGCTTT CCGGACTTCT CCATCTCCAG CAACCTAATC 
AGTGAGGAGA GGGAGAGGAA GACTTCGCAC TGTCAGGACC AGGCGGACGA GGTGACGTCG 
GGCTTGTCGA CGGCCGGCAG CAGCTGTCAG CGGGAGCTCC TGGTGCTGAA CTGTGACCTG 
GAGCCGGACT GTGTGGACGG AGAGCTGAGA GCCGCGCTGC AGTGGATCTC AGCATCTGAA 
CTCGGCGTCC CGGCGCTTTA CTTCAGAAAG ACTCACCAGC ACAATCTTAC AAAGCTCCAC 
AGAGTGCTGC AGTTGGCAGG TCAGAAGGCT TGGCGTGTTG GAGACTTGTT CAGCGCCGTG 
GCTCAGTTCT GTCAGCTCCA CCAGGACCTG GAGCTGAAAG GACGGCCGCT GCCCAGCCTG 
TTTGACTGGA TCCTGAAGAC CAAACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sphkap ( XM_009293857.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292132.1 Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X2, mRNA.

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CloneID	ODa48352
Clone ID Related Accession (Same CDS sequence)	XM_009293857.2 , XM_009293858.2 , XM_009293857.3
Accession Version	XM_009293857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293857.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAGG GATCTGAGTC CGCGAACACA CAGGAAGTCA AATCTGAGAG CACCCTTGGG 
TCCTCGCTCT CCGCCTGCAA GAAGGTTTTG TGCAGTAACA GTGTTCTGGA TTCGTCTGAG 
TACTGGCTGA AGAATGATAA GACGCTCTGC AGAATTGGAT TTCTGGAGGA CCAGCATGAC 
AGCGGCTGCC CGACAATTTG CTTTGTAAAC CTGGACAGAC ATACTTCAGA TTGGCATGAT 
GACAACTACA TCAAGAAGCT GGCCTCGGTG TGCCCAGAGC TGCCACGGTT AATTGAGTCT 
CTCGGAGTGC GGCAACCCAA AGAAAATGAG ATTCTGCTTC TCAGCAGCCT AGAGCCACCG 
GACTCCTGCC AGCATGACCC CAGCCACCCA CAGAGCCCGA GAACAGCGGA TGTCTGTCTG 
GTGCATTGTT CATGTGGACG CCGTCCCACC CAAACGAGCA GTATCATTTT TGAGATTAAC 
AAGTTCCTGA TTGGACTACA GTCAGGGCAG GAGAGGCAGA AGCATGGCCG ATTGGCTGGA 
CAACGAGCTG CTGAAGATGA CACCAATCGC TCTGTTTCGT CGATTGAAGA AGATTTCCTC 
ACTGCCTCAG AGCAGCTTGG GGAAGACAGC GAGGAAGACC CTTTCAGGAA TGATCCTGAG 
AATTCTCTCA TGCCCGAGTC TGTTAAGGTG TTGAGAGCAG CCCACGCTCA AAAAAGGAGT 
GAAATAGAGA GAGAGGACAG TGAGGACTCT GAAACCCTCT GCACCTCTTC CAACTCCCAA 
AAGCTTTCAA GAACATATTC CGCCCCTGCA AGAGGCCGAC CCACCACCCC AAAACAAGTC 
AAGGAGTCAG CCGGTCACTA CGCCACCAAT CTGGCTGAGT CAGTCCTGCA AGATGCTTTT 
ATTCGTTTGT CACAAGATGA GCCATCATTT GTTGCAGAAG CAGCAGTGAG TGTGTCAAGT 
GATATAAGCC ATTCCACTGA GGCACCACAG AGGGCTCGAG CTTGCTCATT CGAACTTCCT 
AAAATTGTGA TTGTCCAAAG TCCTGATAAC AGTGAGGAGG TAACAGAATG GCCGGAAGTG 
CAGTCACATG CAACATCAGA GCATGATAGT GGAAATAAGG CCAAAGCAAA GCATGGTACT 
CATCCCCCGC ACAACTCGCC AGTTGGACAC CCTGCTAAGC CATTGGAAAT AGCTTTGGCA 
TGTGCTGCAA GTGTCATTGG TACCATTACC ACCCCTCAAG TCACAGAACA ACTCACACTA 
GAGTCAGGAG AGGAATATGA GTGTGAAGAT GAAGAGGAAG AAAGTGAAAC TGATCAAGGG 
GAGTATTCGT TTTCATCTGC GGTATGTGGC ATGTCTCAGG TCGCTGGAGC TGTAGCTGCG 
GTCGATCTAG CAGATGACTC TGGGGACAAT GAAGACTTGG CAGACATGTA TTCCGCCTCT 
ATGGGACTTC TGTCAGTTGC CCAAGCTTCA ACTGCAATAC CTCTTCACTG TAGCATAGCA 
GAGGGCACCA GCGTTGAGGC CTTTCGAGCC AATGTGGCTG AAGTTCTCCT CAGGGAAGCT 
TCAGCTGTAT TCACTCACAG ACAAAGTTAT TCAAGTGTTG CAAATTTCCT TGAGACCACA 
CATAACAAGA TAGTGGATGG CATCACAAAT CCAAGAAGAC CGTATCAGGA GCAACAAGAT 
GTGGATAACT TCACTCAAGA AATATCTGAC AGTATCTTTC AGTATGCCCT TGAGAAGGCG 
GAGAAGAGAA AAGAACTTGA GGGTCCTGGA AAAGATGCCC CTAACATTGA GGGTTTCCTC 
TCGGATTGTG TGAATAATTT GCTCTTTGAT GTACTGTGTG TAACATCAAG GAAAATAAGT 
GACATTTCAA ATTGTAATAT GGAATCATGT GATGGGCAGG AAGGCAGTGT CGGCTATAGG 
GCATATGAGG CTGATTCCAA AGCTGGCAGG GAAGCATTGA GCCAATTGCA GCATTTGATT 
GAGCCAAGCC AGGCTTATAA TCATGGTGAA AGGCGAAGCA GTGTGGAGAA GTTATCTGCT 
CTAATAGGAA AGAGAGAGAG AGAGCAGAAA CACATACAGG AGGAAAGAGA AAATGAAGCC 
AGACTGAAGG AACCCTACAG ACTTGCTCTG AACAGAGTGA CAGCCACTAT GGTCAAAGAG 
CAGTCTGACC TGCAAGGGCA GCTGGGCAGG AGTGACAAGA ATGCAGACTC TGCGCTCCCT 
TCAGCAGAAA GCTCACCACT TCACATGCAA CGAGGGAGGG CAGGAGGAGA GAGCGAGACC 
AGGCAGCAGT CTTTATCGTC CTCTTCTGGA GCACTTGTGG CACTTAAAAT GGATTCGGGG 
GAGTCCAAAA CTCCTGTCAC GTGCTTTGCT GAAGATTTGG CGACCACAGT GGTCTCCATG 
GCTACAGAGC TGGCAGCGAT ATGCTTGGAG AACTCAAGTG GAAAGCAGCC GTGGTTCTGC 
GCATTAAATG GTGGCTCGGA AGGTCCAGAA GGGCTGCTTC TGCCCTGCCG CACAGCTGCA 
GCTCTCCGCC GAAAGGAGAC ACAAAATGGC ACCTCGGTCA CCAAGAAGCA CAGACCGCCA 
CGTCTCAGTG AAATCAAACG TAAAACCGAA GAACAGCCCG AACTCATGGA GCGCCTGGTC 
AACCGTGTTG TAGATGAAAC CGTTAACTTA GATGAACCCA CAACCACTGA TCCATTCGCG 
CTCTTTGCAT CAGAGGTCAC TGCCAGGATA ATGAACTGCC CAGAATTAAG TGTAGTGGAC 
ACTTCCAAAT CTGGGCAGTC GTCCCGCAGC CGCCTGCAGT GTGAGAGATG GAGTAGCAGG 
GGGAAGGCAT CAAGCTATGA GAGCATTCCA GAGGAGGATG CAGATCCCTC CGGGACATCA 
AATACTTTGG GTCCAGGAAA CAGGCTTGGG CATAATTTGA GCCGAGGAAG CTCTATCTCC 
AAGCAGTCCA GCTGTGAGAG CATTACGGAT GAGTTCTCTC GCTTCATGGT GAACCAGATG 
GAGACAGAGG GTCGAGGGTT TGACCTTCTG CTGGACTACT ATGCTGGGAA AAATGCCAGC 
AGCATCCTTG CAGCAGCTGT TCAGCAGGCT GCCAGTAAAA AAAACGGACA CCTCAATGTG 
CGTACATCAT CTTGTCTCTC GAAGCAGTCC AGCACTGAGA GTATCACAGA AGAGTTTTAT 
CGCTTCATGC TGAAAGATAT GGACAAGGAA AACAAGGACT ACAGTATGAC CAAGACCAAA 
GAGTGGAGTA ATAGCCTGCT ACCCCCGTCC CCCAGAACCC CTTTTTGTAT CCGTCAGTCT 
TCTGTTCCTG ACAGGCGGTC TTCAGATTCC AGACTAACTG TAAACTCGCC TATCAAGGCC 
AATTCATTCG ATGGCTTTGC TCAAAATGTA CACGGGGACT CGCTTAACAT CTATCCAACT 
AACTCTGTGT CGTCATCTGG ACTCTGCAAG TCCGATTCCT GCTTGTATAA ACGCGGCCAA 
ACAGACCAAA TCACAGACAT GCTGATTCAT GAAACCTGGT CCAGCTCTAT CGAATCTCTA 
ATGCGCAAGA ATAAGATAAT TGCCGAGCCC TCAGAGGACA GTCTGGAACT CGATTCTGCA 
GACTCACAGC CACATGTACA GCAGTTTGCT AATCGTCTAG CGGCAGACAT CGTAGAGAGT 
GGCAAATCAC AGCTTGGAGG CCAGCAGGAT GTCACTGCGG CTGCTTGTCA GCCACATATT 
CCAGTTGGAG AAAGGAGACA CGGCTTTAAA CATTCCCGTT GCAACAACAG TGGAAACCGG 
CCAGGCGTGG AGCATCAGGA AAACAGCTGT AGCTCTTACA GTTCCTCCTG TGTGAGGCAG 
GCATGGCGCG GACAGAGAGA GGTACCCTTG ATTCACATTG AACCAGATCA AAGAGAAGAG 
GCCTCGGAAG AGAAGGGAGG GGTTGAGACC CACCACAGGG AGGCATCCCA TCAGACCCAG 
CAACAAAGTG GCAAAGGGAG TGAGACAGCA ACCAAAAGCA GCAGTGACTC AGACAGGGTC 
TCCTCAGTGG CCACAGTGCC CCCTGCTGGT GTGGAGGTGG AGCGGCGGTC TCTCAGCGCT 
AGCAGTGAGG AAAGTGGCTC AGGCAGCTGG GCACAGATTG CCACTGAAGA CGACCCCCAG 
GAGGAGACCA CCAGTAGCTT TATCCAGCTA AGCGAGGGAA ATGGGAACAG CAGCACTTCT 
AGTCTGGGAC TGGCCGACCT AGAGGGCTTT CCGGACTTCT CCATCTCCAG CAACCTAATC 
AGTGAGGAGA GGGAGAGGAA GACTTCGCAC TGTCAGGACC AGGCGGACGA GGTGACGTCG 
GGCTTGTCGA CGGCCGGCAG CAGCTGTCAG CGGGAGCTCC TGGTGCTGAA CTGTGACCTG 
GAGCCGGACT GTGTGGACGG AGAGCTGAGA GCCGCGCTGC AGTGGATCTC AGCATCTGAA 
CTCGGCGTCC CGGCGCTTTA CTTCAGAAAG ACTCACCAGC ACAATCTTAC AAAGCTCCAC 
AGAGTGCTGC AGTTGGCAGG TCAGAAGGCT TGGCGTGTTG GAGACTTGTT CAGCGCCGTG 
GCTCAGTTCT GTCAGCTCCA CCAGGACCTG GAGCTGAAAG GACGGCCGCT GCCCAGCCTG 
TTTGACTGGA TCCTGAAGAC CAAACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292132.1
CDS	364..5013
Translation	MFEGSESANTQEVKSESTLGSSLSACKKVLCSNSVLDSSEYWLKNDKTLCRIGFLEDQHDSGCPTICFVNLDRHTSDWHDDNYIKKLASVCPELPRLIESLGVRQPKENEILLLSSLEPPDSCQHDPSHPQSPRTADVCLVHCSCGRRPTQTSSIIFEINKFLIGLQSGQERQKHGRLAGQRAAEDDTNRSVSSIEEDFLTASEQLGEDSEEDPFRNDPENSLMPESVKVLRAAHAQKRSEIEREDSEDSETLCTSSNSQKLSRTYSAPARGRPTTPKQVKESAGHYATNLAESVLQDAFIRLSQDEPSFVAEAAVSVSSDISHSTEAPQRARACSFELPKIVIVQSPDNSEEVTEWPEVQSHATSEHDSGNKAKAKHGTHPPHNSPVGHPAKPLEIALACAASVIGTITTPQVTEQLTLESGEEYECEDEEEESETDQGEYSFSSAVCGMSQVAGAVAAVDLADDSGDNEDLADMYSASMGLLSVAQASTAIPLHCSIAEGTSVEAFRANVAEVLLREASAVFTHRQSYSSVANFLETTHNKIVDGITNPRRPYQEQQDVDNFTQEISDSIFQYALEKAEKRKELEGPGKDAPNIEGFLSDCVNNLLFDVLCVTSRKISDISNCNMESCDGQEGSVGYRAYEADSKAGREALSQLQHLIEPSQAYNHGERRSSVEKLSALIGKREREQKHIQEERENEARLKEPYRLALNRVTATMVKEQSDLQGQLGRSDKNADSALPSAESSPLHMQRGRAGGESETRQQSLSSSSGALVALKMDSGESKTPVTCFAEDLATTVVSMATELAAICLENSSGKQPWFCALNGGSEGPEGLLLPCRTAAALRRKETQNGTSVTKKHRPPRLSEIKRKTEEQPELMERLVNRVVDETVNLDEPTTTDPFALFASEVTARIMNCPELSVVDTSKSGQSSRSRLQCERWSSRGKASSYESIPEEDADPSGTSNTLGPGNRLGHNLSRGSSISKQSSCESITDEFSRFMVNQMETEGRGFDLLLDYYAGKNASSILAAAVQQAASKKNGHLNVRTSSCLSKQSSTESITEEFYRFMLKDMDKENKDYSMTKTKEWSNSLLPPSPRTPFCIRQSSVPDRRSSDSRLTVNSPIKANSFDGFAQNVHGDSLNIYPTNSVSSSGLCKSDSCLYKRGQTDQITDMLIHETWSSSIESLMRKNKIIAEPSEDSLELDSADSQPHVQQFANRLAADIVESGKSQLGGQQDVTAAACQPHIPVGERRHGFKHSRCNNSGNRPGVEHQENSCSSYSSSCVRQAWRGQREVPLIHIEPDQREEASEEKGGVETHHREASHQTQQQSGKGSETATKSSSDSDRVSSVATVPPAGVEVERRSLSASSEESGSGSWAQIATEDDPQEETTSSFIQLSEGNGNSSTSSLGLADLEGFPDFSISSNLISEERERKTSHCQDQADEVTSGLSTAGSSCQRELLVLNCDLEPDCVDGELRAALQWISASELGVPALYFRKTHQHNLTKLHRVLQLAGQKAWRVGDLFSAVAQFCQLHQDLELKGRPLPSLFDWILKTKR

Target ORF information:

RefSeq Version	XM_009293857.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293857.3

ORF Insert Sequence:

ATGTTTGAGG GATCTGAGTC CGCGAACACA CAGGAAGTCA AATCTGAGAG CACCCTTGGG 
TCCTCGCTCT CCGCCTGCAA GAAGGTTTTG TGCAGTAACA GTGTTCTGGA TTCGTCTGAG 
TACTGGCTGA AGAATGATAA GACGCTCTGC AGAATTGGAT TTCTGGAGGA CCAGCATGAC 
AGCGGCTGCC CGACAATTTG CTTTGTAAAC CTGGACAGAC ATACTTCAGA TTGGCATGAT 
GACAACTACA TCAAGAAGCT GGCCTCGGTG TGCCCAGAGC TGCCACGGTT AATTGAGTCT 
CTCGGAGTGC GGCAACCCAA AGAAAATGAG ATTCTGCTTC TCAGCAGCCT AGAGCCACCG 
GACTCCTGCC AGCATGACCC CAGCCACCCA CAGAGCCCGA GAACAGCGGA TGTCTGTCTG 
GTGCATTGTT CATGTGGACG CCGTCCCACC CAAACGAGCA GTATCATTTT TGAGATTAAC 
AAGTTCCTGA TTGGACTACA GTCAGGGCAG GAGAGGCAGA AGCATGGCCG ATTGGCTGGA 
CAACGAGCTG CTGAAGATGA CACCAATCGC TCTGTTTCGT CGATTGAAGA AGATTTCCTC 
ACTGCCTCAG AGCAGCTTGG GGAAGACAGC GAGGAAGACC CTTTCAGGAA TGATCCTGAG 
AATTCTCTCA TGCCCGAGTC TGTTAAGGTG TTGAGAGCAG CCCACGCTCA AAAAAGGAGT 
GAAATAGAGA GAGAGGACAG TGAGGACTCT GAAACCCTCT GCACCTCTTC CAACTCCCAA 
AAGCTTTCAA GAACATATTC CGCCCCTGCA AGAGGCCGAC CCACCACCCC AAAACAAGTC 
AAGGAGTCAG CCGGTCACTA CGCCACCAAT CTGGCTGAGT CAGTCCTGCA AGATGCTTTT 
ATTCGTTTGT CACAAGATGA GCCATCATTT GTTGCAGAAG CAGCAGTGAG TGTGTCAAGT 
GATATAAGCC ATTCCACTGA GGCACCACAG AGGGCTCGAG CTTGCTCATT CGAACTTCCT 
AAAATTGTGA TTGTCCAAAG TCCTGATAAC AGTGAGGAGG TAACAGAATG GCCGGAAGTG 
CAGTCACATG CAACATCAGA GCATGATAGT GGAAATAAGG CCAAAGCAAA GCATGGTACT 
CATCCCCCGC ACAACTCGCC AGTTGGACAC CCTGCTAAGC CATTGGAAAT AGCTTTGGCA 
TGTGCTGCAA GTGTCATTGG TACCATTACC ACCCCTCAAG TCACAGAACA ACTCACACTA 
GAGTCAGGAG AGGAATATGA GTGTGAAGAT GAAGAGGAAG AAAGTGAAAC TGATCAAGGG 
GAGTATTCGT TTTCATCTGC GGTATGTGGC ATGTCTCAGG TCGCTGGAGC TGTAGCTGCG 
GTCGATCTAG CAGATGACTC TGGGGACAAT GAAGACTTGG CAGACATGTA TTCCGCCTCT 
ATGGGACTTC TGTCAGTTGC CCAAGCTTCA ACTGCAATAC CTCTTCACTG TAGCATAGCA 
GAGGGCACCA GCGTTGAGGC CTTTCGAGCC AATGTGGCTG AAGTTCTCCT CAGGGAAGCT 
TCAGCTGTAT TCACTCACAG ACAAAGTTAT TCAAGTGTTG CAAATTTCCT TGAGACCACA 
CATAACAAGA TAGTGGATGG CATCACAAAT CCAAGAAGAC CGTATCAGGA GCAACAAGAT 
GTGGATAACT TCACTCAAGA AATATCTGAC AGTATCTTTC AGTATGCCCT TGAGAAGGCG 
GAGAAGAGAA AAGAACTTGA GGGTCCTGGA AAAGATGCCC CTAACATTGA GGGTTTCCTC 
TCGGATTGTG TGAATAATTT GCTCTTTGAT GTACTGTGTG TAACATCAAG GAAAATAAGT 
GACATTTCAA ATTGTAATAT GGAATCATGT GATGGGCAGG AAGGCAGTGT CGGCTATAGG 
GCATATGAGG CTGATTCCAA AGCTGGCAGG GAAGCATTGA GCCAATTGCA GCATTTGATT 
GAGCCAAGCC AGGCTTATAA TCATGGTGAA AGGCGAAGCA GTGTGGAGAA GTTATCTGCT 
CTAATAGGAA AGAGAGAGAG AGAGCAGAAA CACATACAGG AGGAAAGAGA AAATGAAGCC 
AGACTGAAGG AACCCTACAG ACTTGCTCTG AACAGAGTGA CAGCCACTAT GGTCAAAGAG 
CAGTCTGACC TGCAAGGGCA GCTGGGCAGG AGTGACAAGA ATGCAGACTC TGCGCTCCCT 
TCAGCAGAAA GCTCACCACT TCACATGCAA CGAGGGAGGG CAGGAGGAGA GAGCGAGACC 
AGGCAGCAGT CTTTATCGTC CTCTTCTGGA GCACTTGTGG CACTTAAAAT GGATTCGGGG 
GAGTCCAAAA CTCCTGTCAC GTGCTTTGCT GAAGATTTGG CGACCACAGT GGTCTCCATG 
GCTACAGAGC TGGCAGCGAT ATGCTTGGAG AACTCAAGTG GAAAGCAGCC GTGGTTCTGC 
GCATTAAATG GTGGCTCGGA AGGTCCAGAA GGGCTGCTTC TGCCCTGCCG CACAGCTGCA 
GCTCTCCGCC GAAAGGAGAC ACAAAATGGC ACCTCGGTCA CCAAGAAGCA CAGACCGCCA 
CGTCTCAGTG AAATCAAACG TAAAACCGAA GAACAGCCCG AACTCATGGA GCGCCTGGTC 
AACCGTGTTG TAGATGAAAC CGTTAACTTA GATGAACCCA CAACCACTGA TCCATTCGCG 
CTCTTTGCAT CAGAGGTCAC TGCCAGGATA ATGAACTGCC CAGAATTAAG TGTAGTGGAC 
ACTTCCAAAT CTGGGCAGTC GTCCCGCAGC CGCCTGCAGT GTGAGAGATG GAGTAGCAGG 
GGGAAGGCAT CAAGCTATGA GAGCATTCCA GAGGAGGATG CAGATCCCTC CGGGACATCA 
AATACTTTGG GTCCAGGAAA CAGGCTTGGG CATAATTTGA GCCGAGGAAG CTCTATCTCC 
AAGCAGTCCA GCTGTGAGAG CATTACGGAT GAGTTCTCTC GCTTCATGGT GAACCAGATG 
GAGACAGAGG GTCGAGGGTT TGACCTTCTG CTGGACTACT ATGCTGGGAA AAATGCCAGC 
AGCATCCTTG CAGCAGCTGT TCAGCAGGCT GCCAGTAAAA AAAACGGACA CCTCAATGTG 
CGTACATCAT CTTGTCTCTC GAAGCAGTCC AGCACTGAGA GTATCACAGA AGAGTTTTAT 
CGCTTCATGC TGAAAGATAT GGACAAGGAA AACAAGGACT ACAGTATGAC CAAGACCAAA 
GAGTGGAGTA ATAGCCTGCT ACCCCCGTCC CCCAGAACCC CTTTTTGTAT CCGTCAGTCT 
TCTGTTCCTG ACAGGCGGTC TTCAGATTCC AGACTAACTG TAAACTCGCC TATCAAGGCC 
AATTCATTCG ATGGCTTTGC TCAAAATGTA CACGGGGACT CGCTTAACAT CTATCCAACT 
AACTCTGTGT CGTCATCTGG ACTCTGCAAG TCCGATTCCT GCTTGTATAA ACGCGGCCAA 
ACAGACCAAA TCACAGACAT GCTGATTCAT GAAACCTGGT CCAGCTCTAT CGAATCTCTA 
ATGCGCAAGA ATAAGATAAT TGCCGAGCCC TCAGAGGACA GTCTGGAACT CGATTCTGCA 
GACTCACAGC CACATGTACA GCAGTTTGCT AATCGTCTAG CGGCAGACAT CGTAGAGAGT 
GGCAAATCAC AGCTTGGAGG CCAGCAGGAT GTCACTGCGG CTGCTTGTCA GCCACATATT 
CCAGTTGGAG AAAGGAGACA CGGCTTTAAA CATTCCCGTT GCAACAACAG TGGAAACCGG 
CCAGGCGTGG AGCATCAGGA AAACAGCTGT AGCTCTTACA GTTCCTCCTG TGTGAGGCAG 
GCATGGCGCG GACAGAGAGA GGTACCCTTG ATTCACATTG AACCAGATCA AAGAGAAGAG 
GCCTCGGAAG AGAAGGGAGG GGTTGAGACC CACCACAGGG AGGCATCCCA TCAGACCCAG 
CAACAAAGTG GCAAAGGGAG TGAGACAGCA ACCAAAAGCA GCAGTGACTC AGACAGGGTC 
TCCTCAGTGG CCACAGTGCC CCCTGCTGGT GTGGAGGTGG AGCGGCGGTC TCTCAGCGCT 
AGCAGTGAGG AAAGTGGCTC AGGCAGCTGG GCACAGATTG CCACTGAAGA CGACCCCCAG 
GAGGAGACCA CCAGTAGCTT TATCCAGCTA AGCGAGGGAA ATGGGAACAG CAGCACTTCT 
AGTCTGGGAC TGGCCGACCT AGAGGGCTTT CCGGACTTCT CCATCTCCAG CAACCTAATC 
AGTGAGGAGA GGGAGAGGAA GACTTCGCAC TGTCAGGACC AGGCGGACGA GGTGACGTCG 
GGCTTGTCGA CGGCCGGCAG CAGCTGTCAG CGGGAGCTCC TGGTGCTGAA CTGTGACCTG 
GAGCCGGACT GTGTGGACGG AGAGCTGAGA GCCGCGCTGC AGTGGATCTC AGCATCTGAA 
CTCGGCGTCC CGGCGCTTTA CTTCAGAAAG ACTCACCAGC ACAATCTTAC AAAGCTCCAC 
AGAGTGCTGC AGTTGGCAGG TCAGAAGGCT TGGCGTGTTG GAGACTTGTT CAGCGCCGTG 
GCTCAGTTCT GTCAGCTCCA CCAGGACCTG GAGCTGAAAG GACGGCCGCT GCCCAGCCTG 
TTTGACTGGA TCCTGAAGAC CAAACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sphkap ( XM_009293859.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292134.1 Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa48353
Clone ID Related Accession (Same CDS sequence)	XM_009293859.3 , XM_009293859.2
Accession Version	XM_009293859.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein SPHKAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293859.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGCAA ACCTGCTCAG CGTCCTCCAA AACTTCACAG AGAGTAATTT CCAATCATCT 
GCCATGTTTG AGGGATCTGA GTCCGCGAAC ACACAGGAAG TCAAATCTGA GAGCACCCTT 
GGGTCCTCGC TCTCCGCCTG CAAGAAGGTT TTGTGCAGTA ACAGTGTTCT GGATTCGTCT 
GAGTACTGGC TGAAGAATGA TAAGACGCTC TGCAGAATTG GATTTCTGGA GGACCAGCAT 
GACAGCGGCT GCCCGACAAT TTGCTTTGTA AACCTGGACA GACATACTTC AGATTGGCAT 
GATGACAACT ACATCAAGAA GCTGGCCTCG GTGTGCCCAG AGCTGCCACG GTTAATTGAG 
TCTCTCGGAG TGCGGCAACC CAAAGAAAAT GAGATTCTGC TTCTCAGCAG CCTAGAGCCA 
CCGGACTCCT GCCAGCATGA CCCCAGCCAC CCACAGAGCC CGAGAACAGC GGATGTCTGT 
CTGGTGCATT GTTCATGTGG ACGCCGTCCC ACCCAAACGA GCAGTATCAT TTTTGAGATT 
AACAAGTTCC TGATTGGACT ACAGTCAGGG CAGGAGAGGC AGAAGCATGG CCGATTGGCT 
GGACAACGAG CTGCTGAAGA TGACACCAAT CGCTCTGTTT CGTCGATTGA AGAAGATTTC 
CTCACTGCCT CAGAGCAGCT TGGGGAAGAC AGCGAGGAAG ACCCTTTCAG GAATGATCCT 
GAGAATTCTC TCATGCCCGA GTCTGTTAAG GTGTTGAGAG CAGCCCACGC TCAAAAAAGG 
AGTGAAATAG AGAGAGAGGA CAGTGAGGAC TCTGAAACCC TCTGCACCTC TTCCAACTCC 
CAAAAGCTTT CAAGAACATA TTCCGCCCCT GCAAGAGGCC GACCCACCAC CCCAAAACAA 
GTCAAGGAGT CAGCCGGTCA CTACGCCACC AATCTGGCTG AGTCAGTCCT GCAAGATGCT 
TTTATTCGTT TGTCACAAGA TGAGCCATCA TTTGTTGCAG AAGCAGCAGT GAGTGTGTCA 
AGTGATATAA GCCATTCCAC TGAGGCACCA CAGAGGGCTC GAGCTTGCTC ATTCGAACTT 
CCTAAAATTG TGATTGTCCA AAGTCCTGAT AACAGTGAGG AGGTAACAGA ATGGCCGGAA 
GTGCAGTCAC ATGCAACATC AGAGCATGAT AGTGGAAATA AGGCCAAAGC AAAGCATGGT 
ACTCATCCCC CGCACAACTC GCCAGTTGGA CACCCTGCTA AGCCATTGGA AATAGCTTTG 
GCATGTGCTG CAAGTGTCAT TGGTACCATT ACCACCCCTC AAGTCACAGA ACAACTCACA 
CTAGAGTCAG GAGAGGAATA TGAGTGTGAA GATGAAGAGG AAGAAAGTGA AACTGATCAA 
GGGGAGTATT CGTTTTCATC TGCGGTATGT GGCATGTCTC AGGTCGCTGG AGCTGTAGCT 
GCGGTCGATC TAGCAGATGA CTCTGGGGAC AATGAAGACT TGGCAGACAT GTATTCCGCC 
TCTATGGGAC TTCTGTCAGT TGCCCAAGCT TCAACTGCAA TACCTCTTCA CTGTAGCATA 
GCAGAGGGCA CCAGCGTTGA GGCCTTTCGA GCCAATGTGG CTGAAGTTCT CCTCAGGGAA 
GCTTCAGCTG TATTCACTCA CAGACAAAGT TATTCAAGTG TTGCAAATTT CCTTGAGACC 
ACACATAACA AGATAGTGGA TGGCATCACA AATCCAAGAA GACCGTATCA GGAGCAACAA 
GATGTGGATA ACTTCACTCA AGAAATATCT GACAGTATCT TTCAGTATGC CCTTGAGAAG 
GCGGAGAAGA GAAAAGAACT TGAGGGTCCT GGAAAAGATG CCCCTAACAT TGAGGGTTTC 
CTCTCGGATT GTGTGAATAA TTTGCTCTTT GATGTACTGT GTGTAACATC AAGGAAAATA 
AGTGACATTT CAAATTGTAA TATGGAATCA TGTGATGGGC AGGAAGGCAG TGTCGGCTAT 
AGGGCATATG AGGCTGATTC CAAAGCTGGC AGGGAAGCAT TGAGCCAATT GCAGCATTTG 
ATTGAGCCAA GCCAGGCTTA TAATCATGGT GAAAGGCGAA GCAGTGTGGA GAAGTTATCT 
GCTCTAATAG GAAAGAGAGA GAGAGAGCAG AAACACATAC AGGAGGAAAG AGAAAATGAA 
GCCAGACTGA AGGAACCCTA CAGACTTGCT CTGAACAGAG TGACAGCCAC TATGGTCAAA 
GAGCAGTCTG ACCTGCAAGG GCAGCTGGGC AGGAGTGACA AGAATGCAGA CTCTGCGCTC 
CCTTCAGCAG AAAGCTCACC ACTTCACATG CAACGAGGGA GGGCAGGAGG AGAGAGCGAG 
ACCAGGCAGC AGTCTTTATC GTCCTCTTCT GGAGCACTTG TGGCACTTAA AATGGATTCG 
GGGGAGTCCA AAACTCCTGT CACGTGCTTT GCTGAAGATT TGGCGACCAC AGTGGTCTCC 
ATGGCTACAG AGCTGGCAGC GATATGCTTG GAGAACTCAA GTGGAAAGCA GCCGTGGTTC 
TGCGCATTAA ATGGTGGCTC GGAAGGTCCA GAAGGGCTGC TTCTGCCCTG CCGCACAGCT 
GCAGCTCTCC GCCGAAAGGA GACACAAAAT GGCACCTCGG TCACCAAGAA GCACAGACCG 
CCACGTCTCA GTGAAATCAA ACGTAAAACC GAAGAACAGC CCGAACTCAT GGAGCGCCTG 
GTCAACCGTG TTGTAGATGA AACCGTTAAC TTAGATGAAC CCACAACCAC TGATCCATTC 
GCGCTCTTTG CATCAGAGGT CACTGCCAGG ATAATGAACT GCCCAGAATT AAGTGTAGTG 
GACACTTCCA AATCTGGGCA GTCGTCCCGC AGCCGCCTGC AGTGTGAGAG ATGGAGTAGC 
AGGGGGAAGG CATCAAGCTA TGAGAGCATT CCAGAGGAGG ATGCAGATCC CTCCGGGACA 
TCAAATACTT TGGGTCCAGG AAACAGGCTT GGGCATAATT TGAGCCGAGG AAGCTCTATC 
TCCAAGCAGT CCAGCTGTGA GAGCATTACG GATGAGTTCT CTCGCTTCAT GGTGAACCAG 
ATGGAGACAG AGGGTCGAGG GTTTGACCTT CTGCTGGACT ACTATGCTGG GAAAAATGCC 
AGCAGCATCC TTGCAGCAGC TGTTCAGCAG GCTGCCAGTA AAAAAAACGG ACACCTCAAT 
GTGCGTACAT CATCTTGTCT CTCGAAGCAG TCCAGCACTG AGAGTATCAC AGAAGAGTTT 
TATCGCTTCA TGCTGAAAGA TATGGACAAG GAAAACAAGG ACTACAGTAT GACCAAGACC 
AAAGAGTGGA GTAATAGCCT GCTACCCCCG TCCCCCAGAA CCCCTTTTTG TATCCGTCAG 
TCTTCTGTTC CTGACAGGCG GTCTTCAGAT TCCAGACTAA CTGTAAACTC GCCTATCAAG 
GCCAATTCAT TCGATGGCTT TGCTCAAAAT GTACACGGGG ACTCGCTTAA CATCTATCCA 
ACTAACTCTG TGTCGTCATC TGGACTCTGC AAGTCCGATT CCTGCTTGTA TAAACGCGGC 
CAAACAGACC AAATCACAGA CATGCTGATT CATGAAACCT GGTCCAGCTC TATCGAATCT 
CTAATGCGCA AGAATAAGAT AATTGCCGAG CCCTCAGAGG ACAGTCTGGA ACTCGATTCT 
GCAGACTCAC AGCCACATGT ACAGCAGTTT GCTAATCGTC TAGCGGCAGA CATCGTAGAG 
AGTGGCAAAT CACAGCTTGG AGGCCAGCAG GATGTCACTG CGGCTGCTTG TCAGCCACAT 
ATTCCAGTTG GAGAAAGGAG ACACGGCTTT AAACATTCCC GTTGCAACAA CAGTGGAAAC 
CGGCCAGGCG TGGAGCATCA GGAAAACAGC TGTAGCTCTT ACAGTTCCTC CTGTGTGAGG 
CAGGCATGGC GCGGACAGAG AGAGGTACCC TTGATTCACA TTGAACCAGA TCAAAGAGAA 
GAGGCCTCGG AAGAGAAGGG AGGGGTTGAG ACCCACCACA GGGAGGCATC CCATCAGACC 
CAGCAACAAA GTGGCAAAGG GAGTGAGACA GCAACCAAAA GCAGAAATGG GAACAGCAGC 
ACTTCTAGTC TGGGACTGGC CGACCTAGAG GGCTTTCCGG ACTTCTCCAT CTCCAGCAAC 
CTAATCAGTG AGGAGAGGGA GAGGAAGACT TCGCACTGTC AGGACCAGGC GGACGAGGTG 
ACGTCGGGCT TGTCGACGGC CGGCAGCAGC TGTCAGCGGG AGCTCCTGGT GCTGAACTGT 
GACCTGGAGC CGGACTGTGT GGACGGAGAG CTGAGAGCCG CGCTGCAGTG GATCTCAGCA 
TCTGAACTCG GCGTCCCGGC GCTTTACTTC AGAAAGACTC ACCAGCACAA TCTTACAAAG 
CTCCACAGAG TGCTGCAGTT GGCAGGTCAG AAGGCTTGGC GTGTTGGAGA CTTGTTCAGC 
GCCGTGGCTC AGTTCTGTCA GCTCCACCAG GACCTGGAGC TGAAAGGACG GCCGCTGCCC 
AGCCTGTTTG ACTGGATCCT GAAGACCAAA CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292134.1
CDS	434..4969
Translation	MLANLLSVLQNFTESNFQSSAMFEGSESANTQEVKSESTLGSSLSACKKVLCSNSVLDSSEYWLKNDKTLCRIGFLEDQHDSGCPTICFVNLDRHTSDWHDDNYIKKLASVCPELPRLIESLGVRQPKENEILLLSSLEPPDSCQHDPSHPQSPRTADVCLVHCSCGRRPTQTSSIIFEINKFLIGLQSGQERQKHGRLAGQRAAEDDTNRSVSSIEEDFLTASEQLGEDSEEDPFRNDPENSLMPESVKVLRAAHAQKRSEIEREDSEDSETLCTSSNSQKLSRTYSAPARGRPTTPKQVKESAGHYATNLAESVLQDAFIRLSQDEPSFVAEAAVSVSSDISHSTEAPQRARACSFELPKIVIVQSPDNSEEVTEWPEVQSHATSEHDSGNKAKAKHGTHPPHNSPVGHPAKPLEIALACAASVIGTITTPQVTEQLTLESGEEYECEDEEEESETDQGEYSFSSAVCGMSQVAGAVAAVDLADDSGDNEDLADMYSASMGLLSVAQASTAIPLHCSIAEGTSVEAFRANVAEVLLREASAVFTHRQSYSSVANFLETTHNKIVDGITNPRRPYQEQQDVDNFTQEISDSIFQYALEKAEKRKELEGPGKDAPNIEGFLSDCVNNLLFDVLCVTSRKISDISNCNMESCDGQEGSVGYRAYEADSKAGREALSQLQHLIEPSQAYNHGERRSSVEKLSALIGKREREQKHIQEERENEARLKEPYRLALNRVTATMVKEQSDLQGQLGRSDKNADSALPSAESSPLHMQRGRAGGESETRQQSLSSSSGALVALKMDSGESKTPVTCFAEDLATTVVSMATELAAICLENSSGKQPWFCALNGGSEGPEGLLLPCRTAAALRRKETQNGTSVTKKHRPPRLSEIKRKTEEQPELMERLVNRVVDETVNLDEPTTTDPFALFASEVTARIMNCPELSVVDTSKSGQSSRSRLQCERWSSRGKASSYESIPEEDADPSGTSNTLGPGNRLGHNLSRGSSISKQSSCESITDEFSRFMVNQMETEGRGFDLLLDYYAGKNASSILAAAVQQAASKKNGHLNVRTSSCLSKQSSTESITEEFYRFMLKDMDKENKDYSMTKTKEWSNSLLPPSPRTPFCIRQSSVPDRRSSDSRLTVNSPIKANSFDGFAQNVHGDSLNIYPTNSVSSSGLCKSDSCLYKRGQTDQITDMLIHETWSSSIESLMRKNKIIAEPSEDSLELDSADSQPHVQQFANRLAADIVESGKSQLGGQQDVTAAACQPHIPVGERRHGFKHSRCNNSGNRPGVEHQENSCSSYSSSCVRQAWRGQREVPLIHIEPDQREEASEEKGGVETHHREASHQTQQQSGKGSETATKSRNGNSSTSSLGLADLEGFPDFSISSNLISEERERKTSHCQDQADEVTSGLSTAGSSCQRELLVLNCDLEPDCVDGELRAALQWISASELGVPALYFRKTHQHNLTKLHRVLQLAGQKAWRVGDLFSAVAQFCQLHQDLELKGRPLPSLFDWILKTKR

Target ORF information:

RefSeq Version	XM_009293859.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293859.3

ORF Insert Sequence:

ATGCTAGCAA ACCTGCTCAG CGTCCTCCAA AACTTCACAG AGAGTAATTT CCAATCATCT 
GCCATGTTTG AGGGATCTGA GTCCGCGAAC ACACAGGAAG TCAAATCTGA GAGCACCCTT 
GGGTCCTCGC TCTCCGCCTG CAAGAAGGTT TTGTGCAGTA ACAGTGTTCT GGATTCGTCT 
GAGTACTGGC TGAAGAATGA TAAGACGCTC TGCAGAATTG GATTTCTGGA GGACCAGCAT 
GACAGCGGCT GCCCGACAAT TTGCTTTGTA AACCTGGACA GACATACTTC AGATTGGCAT 
GATGACAACT ACATCAAGAA GCTGGCCTCG GTGTGCCCAG AGCTGCCACG GTTAATTGAG 
TCTCTCGGAG TGCGGCAACC CAAAGAAAAT GAGATTCTGC TTCTCAGCAG CCTAGAGCCA 
CCGGACTCCT GCCAGCATGA CCCCAGCCAC CCACAGAGCC CGAGAACAGC GGATGTCTGT 
CTGGTGCATT GTTCATGTGG ACGCCGTCCC ACCCAAACGA GCAGTATCAT TTTTGAGATT 
AACAAGTTCC TGATTGGACT ACAGTCAGGG CAGGAGAGGC AGAAGCATGG CCGATTGGCT 
GGACAACGAG CTGCTGAAGA TGACACCAAT CGCTCTGTTT CGTCGATTGA AGAAGATTTC 
CTCACTGCCT CAGAGCAGCT TGGGGAAGAC AGCGAGGAAG ACCCTTTCAG GAATGATCCT 
GAGAATTCTC TCATGCCCGA GTCTGTTAAG GTGTTGAGAG CAGCCCACGC TCAAAAAAGG 
AGTGAAATAG AGAGAGAGGA CAGTGAGGAC TCTGAAACCC TCTGCACCTC TTCCAACTCC 
CAAAAGCTTT CAAGAACATA TTCCGCCCCT GCAAGAGGCC GACCCACCAC CCCAAAACAA 
GTCAAGGAGT CAGCCGGTCA CTACGCCACC AATCTGGCTG AGTCAGTCCT GCAAGATGCT 
TTTATTCGTT TGTCACAAGA TGAGCCATCA TTTGTTGCAG AAGCAGCAGT GAGTGTGTCA 
AGTGATATAA GCCATTCCAC TGAGGCACCA CAGAGGGCTC GAGCTTGCTC ATTCGAACTT 
CCTAAAATTG TGATTGTCCA AAGTCCTGAT AACAGTGAGG AGGTAACAGA ATGGCCGGAA 
GTGCAGTCAC ATGCAACATC AGAGCATGAT AGTGGAAATA AGGCCAAAGC AAAGCATGGT 
ACTCATCCCC CGCACAACTC GCCAGTTGGA CACCCTGCTA AGCCATTGGA AATAGCTTTG 
GCATGTGCTG CAAGTGTCAT TGGTACCATT ACCACCCCTC AAGTCACAGA ACAACTCACA 
CTAGAGTCAG GAGAGGAATA TGAGTGTGAA GATGAAGAGG AAGAAAGTGA AACTGATCAA 
GGGGAGTATT CGTTTTCATC TGCGGTATGT GGCATGTCTC AGGTCGCTGG AGCTGTAGCT 
GCGGTCGATC TAGCAGATGA CTCTGGGGAC AATGAAGACT TGGCAGACAT GTATTCCGCC 
TCTATGGGAC TTCTGTCAGT TGCCCAAGCT TCAACTGCAA TACCTCTTCA CTGTAGCATA 
GCAGAGGGCA CCAGCGTTGA GGCCTTTCGA GCCAATGTGG CTGAAGTTCT CCTCAGGGAA 
GCTTCAGCTG TATTCACTCA CAGACAAAGT TATTCAAGTG TTGCAAATTT CCTTGAGACC 
ACACATAACA AGATAGTGGA TGGCATCACA AATCCAAGAA GACCGTATCA GGAGCAACAA 
GATGTGGATA ACTTCACTCA AGAAATATCT GACAGTATCT TTCAGTATGC CCTTGAGAAG 
GCGGAGAAGA GAAAAGAACT TGAGGGTCCT GGAAAAGATG CCCCTAACAT TGAGGGTTTC 
CTCTCGGATT GTGTGAATAA TTTGCTCTTT GATGTACTGT GTGTAACATC AAGGAAAATA 
AGTGACATTT CAAATTGTAA TATGGAATCA TGTGATGGGC AGGAAGGCAG TGTCGGCTAT 
AGGGCATATG AGGCTGATTC CAAAGCTGGC AGGGAAGCAT TGAGCCAATT GCAGCATTTG 
ATTGAGCCAA GCCAGGCTTA TAATCATGGT GAAAGGCGAA GCAGTGTGGA GAAGTTATCT 
GCTCTAATAG GAAAGAGAGA GAGAGAGCAG AAACACATAC AGGAGGAAAG AGAAAATGAA 
GCCAGACTGA AGGAACCCTA CAGACTTGCT CTGAACAGAG TGACAGCCAC TATGGTCAAA 
GAGCAGTCTG ACCTGCAAGG GCAGCTGGGC AGGAGTGACA AGAATGCAGA CTCTGCGCTC 
CCTTCAGCAG AAAGCTCACC ACTTCACATG CAACGAGGGA GGGCAGGAGG AGAGAGCGAG 
ACCAGGCAGC AGTCTTTATC GTCCTCTTCT GGAGCACTTG TGGCACTTAA AATGGATTCG 
GGGGAGTCCA AAACTCCTGT CACGTGCTTT GCTGAAGATT TGGCGACCAC AGTGGTCTCC 
ATGGCTACAG AGCTGGCAGC GATATGCTTG GAGAACTCAA GTGGAAAGCA GCCGTGGTTC 
TGCGCATTAA ATGGTGGCTC GGAAGGTCCA GAAGGGCTGC TTCTGCCCTG CCGCACAGCT 
GCAGCTCTCC GCCGAAAGGA GACACAAAAT GGCACCTCGG TCACCAAGAA GCACAGACCG 
CCACGTCTCA GTGAAATCAA ACGTAAAACC GAAGAACAGC CCGAACTCAT GGAGCGCCTG 
GTCAACCGTG TTGTAGATGA AACCGTTAAC TTAGATGAAC CCACAACCAC TGATCCATTC 
GCGCTCTTTG CATCAGAGGT CACTGCCAGG ATAATGAACT GCCCAGAATT AAGTGTAGTG 
GACACTTCCA AATCTGGGCA GTCGTCCCGC AGCCGCCTGC AGTGTGAGAG ATGGAGTAGC 
AGGGGGAAGG CATCAAGCTA TGAGAGCATT CCAGAGGAGG ATGCAGATCC CTCCGGGACA 
TCAAATACTT TGGGTCCAGG AAACAGGCTT GGGCATAATT TGAGCCGAGG AAGCTCTATC 
TCCAAGCAGT CCAGCTGTGA GAGCATTACG GATGAGTTCT CTCGCTTCAT GGTGAACCAG 
ATGGAGACAG AGGGTCGAGG GTTTGACCTT CTGCTGGACT ACTATGCTGG GAAAAATGCC 
AGCAGCATCC TTGCAGCAGC TGTTCAGCAG GCTGCCAGTA AAAAAAACGG ACACCTCAAT 
GTGCGTACAT CATCTTGTCT CTCGAAGCAG TCCAGCACTG AGAGTATCAC AGAAGAGTTT 
TATCGCTTCA TGCTGAAAGA TATGGACAAG GAAAACAAGG ACTACAGTAT GACCAAGACC 
AAAGAGTGGA GTAATAGCCT GCTACCCCCG TCCCCCAGAA CCCCTTTTTG TATCCGTCAG 
TCTTCTGTTC CTGACAGGCG GTCTTCAGAT TCCAGACTAA CTGTAAACTC GCCTATCAAG 
GCCAATTCAT TCGATGGCTT TGCTCAAAAT GTACACGGGG ACTCGCTTAA CATCTATCCA 
ACTAACTCTG TGTCGTCATC TGGACTCTGC AAGTCCGATT CCTGCTTGTA TAAACGCGGC 
CAAACAGACC AAATCACAGA CATGCTGATT CATGAAACCT GGTCCAGCTC TATCGAATCT 
CTAATGCGCA AGAATAAGAT AATTGCCGAG CCCTCAGAGG ACAGTCTGGA ACTCGATTCT 
GCAGACTCAC AGCCACATGT ACAGCAGTTT GCTAATCGTC TAGCGGCAGA CATCGTAGAG 
AGTGGCAAAT CACAGCTTGG AGGCCAGCAG GATGTCACTG CGGCTGCTTG TCAGCCACAT 
ATTCCAGTTG GAGAAAGGAG ACACGGCTTT AAACATTCCC GTTGCAACAA CAGTGGAAAC 
CGGCCAGGCG TGGAGCATCA GGAAAACAGC TGTAGCTCTT ACAGTTCCTC CTGTGTGAGG 
CAGGCATGGC GCGGACAGAG AGAGGTACCC TTGATTCACA TTGAACCAGA TCAAAGAGAA 
GAGGCCTCGG AAGAGAAGGG AGGGGTTGAG ACCCACCACA GGGAGGCATC CCATCAGACC 
CAGCAACAAA GTGGCAAAGG GAGTGAGACA GCAACCAAAA GCAGAAATGG GAACAGCAGC 
ACTTCTAGTC TGGGACTGGC CGACCTAGAG GGCTTTCCGG ACTTCTCCAT CTCCAGCAAC 
CTAATCAGTG AGGAGAGGGA GAGGAAGACT TCGCACTGTC AGGACCAGGC GGACGAGGTG 
ACGTCGGGCT TGTCGACGGC CGGCAGCAGC TGTCAGCGGG AGCTCCTGGT GCTGAACTGT 
GACCTGGAGC CGGACTGTGT GGACGGAGAG CTGAGAGCCG CGCTGCAGTG GATCTCAGCA 
TCTGAACTCG GCGTCCCGGC GCTTTACTTC AGAAAGACTC ACCAGCACAA TCTTACAAAG 
CTCCACAGAG TGCTGCAGTT GGCAGGTCAG AAGGCTTGGC GTGTTGGAGA CTTGTTCAGC 
GCCGTGGCTC AGTTCTGTCA GCTCCACCAG GACCTGGAGC TGAAAGGACG GCCGCTGCCC 
AGCCTGTTTG ACTGGATCCT GAAGACCAAA CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sphkap ( XM_009293859.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292134.1 Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X4, mRNA.

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CloneID	ODa48353
Clone ID Related Accession (Same CDS sequence)	XM_009293859.3 , XM_009293859.2
Accession Version	XM_009293859.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein SPHKAP isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293859.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGCAA ACCTGCTCAG CGTCCTCCAA AACTTCACAG AGAGTAATTT CCAATCATCT 
GCCATGTTTG AGGGATCTGA GTCCGCGAAC ACACAGGAAG TCAAATCTGA GAGCACCCTT 
GGGTCCTCGC TCTCCGCCTG CAAGAAGGTT TTGTGCAGTA ACAGTGTTCT GGATTCGTCT 
GAGTACTGGC TGAAGAATGA TAAGACGCTC TGCAGAATTG GATTTCTGGA GGACCAGCAT 
GACAGCGGCT GCCCGACAAT TTGCTTTGTA AACCTGGACA GACATACTTC AGATTGGCAT 
GATGACAACT ACATCAAGAA GCTGGCCTCG GTGTGCCCAG AGCTGCCACG GTTAATTGAG 
TCTCTCGGAG TGCGGCAACC CAAAGAAAAT GAGATTCTGC TTCTCAGCAG CCTAGAGCCA 
CCGGACTCCT GCCAGCATGA CCCCAGCCAC CCACAGAGCC CGAGAACAGC GGATGTCTGT 
CTGGTGCATT GTTCATGTGG ACGCCGTCCC ACCCAAACGA GCAGTATCAT TTTTGAGATT 
AACAAGTTCC TGATTGGACT ACAGTCAGGG CAGGAGAGGC AGAAGCATGG CCGATTGGCT 
GGACAACGAG CTGCTGAAGA TGACACCAAT CGCTCTGTTT CGTCGATTGA AGAAGATTTC 
CTCACTGCCT CAGAGCAGCT TGGGGAAGAC AGCGAGGAAG ACCCTTTCAG GAATGATCCT 
GAGAATTCTC TCATGCCCGA GTCTGTTAAG GTGTTGAGAG CAGCCCACGC TCAAAAAAGG 
AGTGAAATAG AGAGAGAGGA CAGTGAGGAC TCTGAAACCC TCTGCACCTC TTCCAACTCC 
CAAAAGCTTT CAAGAACATA TTCCGCCCCT GCAAGAGGCC GACCCACCAC CCCAAAACAA 
GTCAAGGAGT CAGCCGGTCA CTACGCCACC AATCTGGCTG AGTCAGTCCT GCAAGATGCT 
TTTATTCGTT TGTCACAAGA TGAGCCATCA TTTGTTGCAG AAGCAGCAGT GAGTGTGTCA 
AGTGATATAA GCCATTCCAC TGAGGCACCA CAGAGGGCTC GAGCTTGCTC ATTCGAACTT 
CCTAAAATTG TGATTGTCCA AAGTCCTGAT AACAGTGAGG AGGTAACAGA ATGGCCGGAA 
GTGCAGTCAC ATGCAACATC AGAGCATGAT AGTGGAAATA AGGCCAAAGC AAAGCATGGT 
ACTCATCCCC CGCACAACTC GCCAGTTGGA CACCCTGCTA AGCCATTGGA AATAGCTTTG 
GCATGTGCTG CAAGTGTCAT TGGTACCATT ACCACCCCTC AAGTCACAGA ACAACTCACA 
CTAGAGTCAG GAGAGGAATA TGAGTGTGAA GATGAAGAGG AAGAAAGTGA AACTGATCAA 
GGGGAGTATT CGTTTTCATC TGCGGTATGT GGCATGTCTC AGGTCGCTGG AGCTGTAGCT 
GCGGTCGATC TAGCAGATGA CTCTGGGGAC AATGAAGACT TGGCAGACAT GTATTCCGCC 
TCTATGGGAC TTCTGTCAGT TGCCCAAGCT TCAACTGCAA TACCTCTTCA CTGTAGCATA 
GCAGAGGGCA CCAGCGTTGA GGCCTTTCGA GCCAATGTGG CTGAAGTTCT CCTCAGGGAA 
GCTTCAGCTG TATTCACTCA CAGACAAAGT TATTCAAGTG TTGCAAATTT CCTTGAGACC 
ACACATAACA AGATAGTGGA TGGCATCACA AATCCAAGAA GACCGTATCA GGAGCAACAA 
GATGTGGATA ACTTCACTCA AGAAATATCT GACAGTATCT TTCAGTATGC CCTTGAGAAG 
GCGGAGAAGA GAAAAGAACT TGAGGGTCCT GGAAAAGATG CCCCTAACAT TGAGGGTTTC 
CTCTCGGATT GTGTGAATAA TTTGCTCTTT GATGTACTGT GTGTAACATC AAGGAAAATA 
AGTGACATTT CAAATTGTAA TATGGAATCA TGTGATGGGC AGGAAGGCAG TGTCGGCTAT 
AGGGCATATG AGGCTGATTC CAAAGCTGGC AGGGAAGCAT TGAGCCAATT GCAGCATTTG 
ATTGAGCCAA GCCAGGCTTA TAATCATGGT GAAAGGCGAA GCAGTGTGGA GAAGTTATCT 
GCTCTAATAG GAAAGAGAGA GAGAGAGCAG AAACACATAC AGGAGGAAAG AGAAAATGAA 
GCCAGACTGA AGGAACCCTA CAGACTTGCT CTGAACAGAG TGACAGCCAC TATGGTCAAA 
GAGCAGTCTG ACCTGCAAGG GCAGCTGGGC AGGAGTGACA AGAATGCAGA CTCTGCGCTC 
CCTTCAGCAG AAAGCTCACC ACTTCACATG CAACGAGGGA GGGCAGGAGG AGAGAGCGAG 
ACCAGGCAGC AGTCTTTATC GTCCTCTTCT GGAGCACTTG TGGCACTTAA AATGGATTCG 
GGGGAGTCCA AAACTCCTGT CACGTGCTTT GCTGAAGATT TGGCGACCAC AGTGGTCTCC 
ATGGCTACAG AGCTGGCAGC GATATGCTTG GAGAACTCAA GTGGAAAGCA GCCGTGGTTC 
TGCGCATTAA ATGGTGGCTC GGAAGGTCCA GAAGGGCTGC TTCTGCCCTG CCGCACAGCT 
GCAGCTCTCC GCCGAAAGGA GACACAAAAT GGCACCTCGG TCACCAAGAA GCACAGACCG 
CCACGTCTCA GTGAAATCAA ACGTAAAACC GAAGAACAGC CCGAACTCAT GGAGCGCCTG 
GTCAACCGTG TTGTAGATGA AACCGTTAAC TTAGATGAAC CCACAACCAC TGATCCATTC 
GCGCTCTTTG CATCAGAGGT CACTGCCAGG ATAATGAACT GCCCAGAATT AAGTGTAGTG 
GACACTTCCA AATCTGGGCA GTCGTCCCGC AGCCGCCTGC AGTGTGAGAG ATGGAGTAGC 
AGGGGGAAGG CATCAAGCTA TGAGAGCATT CCAGAGGAGG ATGCAGATCC CTCCGGGACA 
TCAAATACTT TGGGTCCAGG AAACAGGCTT GGGCATAATT TGAGCCGAGG AAGCTCTATC 
TCCAAGCAGT CCAGCTGTGA GAGCATTACG GATGAGTTCT CTCGCTTCAT GGTGAACCAG 
ATGGAGACAG AGGGTCGAGG GTTTGACCTT CTGCTGGACT ACTATGCTGG GAAAAATGCC 
AGCAGCATCC TTGCAGCAGC TGTTCAGCAG GCTGCCAGTA AAAAAAACGG ACACCTCAAT 
GTGCGTACAT CATCTTGTCT CTCGAAGCAG TCCAGCACTG AGAGTATCAC AGAAGAGTTT 
TATCGCTTCA TGCTGAAAGA TATGGACAAG GAAAACAAGG ACTACAGTAT GACCAAGACC 
AAAGAGTGGA GTAATAGCCT GCTACCCCCG TCCCCCAGAA CCCCTTTTTG TATCCGTCAG 
TCTTCTGTTC CTGACAGGCG GTCTTCAGAT TCCAGACTAA CTGTAAACTC GCCTATCAAG 
GCCAATTCAT TCGATGGCTT TGCTCAAAAT GTACACGGGG ACTCGCTTAA CATCTATCCA 
ACTAACTCTG TGTCGTCATC TGGACTCTGC AAGTCCGATT CCTGCTTGTA TAAACGCGGC 
CAAACAGACC AAATCACAGA CATGCTGATT CATGAAACCT GGTCCAGCTC TATCGAATCT 
CTAATGCGCA AGAATAAGAT AATTGCCGAG CCCTCAGAGG ACAGTCTGGA ACTCGATTCT 
GCAGACTCAC AGCCACATGT ACAGCAGTTT GCTAATCGTC TAGCGGCAGA CATCGTAGAG 
AGTGGCAAAT CACAGCTTGG AGGCCAGCAG GATGTCACTG CGGCTGCTTG TCAGCCACAT 
ATTCCAGTTG GAGAAAGGAG ACACGGCTTT AAACATTCCC GTTGCAACAA CAGTGGAAAC 
CGGCCAGGCG TGGAGCATCA GGAAAACAGC TGTAGCTCTT ACAGTTCCTC CTGTGTGAGG 
CAGGCATGGC GCGGACAGAG AGAGGTACCC TTGATTCACA TTGAACCAGA TCAAAGAGAA 
GAGGCCTCGG AAGAGAAGGG AGGGGTTGAG ACCCACCACA GGGAGGCATC CCATCAGACC 
CAGCAACAAA GTGGCAAAGG GAGTGAGACA GCAACCAAAA GCAGAAATGG GAACAGCAGC 
ACTTCTAGTC TGGGACTGGC CGACCTAGAG GGCTTTCCGG ACTTCTCCAT CTCCAGCAAC 
CTAATCAGTG AGGAGAGGGA GAGGAAGACT TCGCACTGTC AGGACCAGGC GGACGAGGTG 
ACGTCGGGCT TGTCGACGGC CGGCAGCAGC TGTCAGCGGG AGCTCCTGGT GCTGAACTGT 
GACCTGGAGC CGGACTGTGT GGACGGAGAG CTGAGAGCCG CGCTGCAGTG GATCTCAGCA 
TCTGAACTCG GCGTCCCGGC GCTTTACTTC AGAAAGACTC ACCAGCACAA TCTTACAAAG 
CTCCACAGAG TGCTGCAGTT GGCAGGTCAG AAGGCTTGGC GTGTTGGAGA CTTGTTCAGC 
GCCGTGGCTC AGTTCTGTCA GCTCCACCAG GACCTGGAGC TGAAAGGACG GCCGCTGCCC 
AGCCTGTTTG ACTGGATCCT GAAGACCAAA CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292134.1
CDS	448..4983
Translation	MLANLLSVLQNFTESNFQSSAMFEGSESANTQEVKSESTLGSSLSACKKVLCSNSVLDSSEYWLKNDKTLCRIGFLEDQHDSGCPTICFVNLDRHTSDWHDDNYIKKLASVCPELPRLIESLGVRQPKENEILLLSSLEPPDSCQHDPSHPQSPRTADVCLVHCSCGRRPTQTSSIIFEINKFLIGLQSGQERQKHGRLAGQRAAEDDTNRSVSSIEEDFLTASEQLGEDSEEDPFRNDPENSLMPESVKVLRAAHAQKRSEIEREDSEDSETLCTSSNSQKLSRTYSAPARGRPTTPKQVKESAGHYATNLAESVLQDAFIRLSQDEPSFVAEAAVSVSSDISHSTEAPQRARACSFELPKIVIVQSPDNSEEVTEWPEVQSHATSEHDSGNKAKAKHGTHPPHNSPVGHPAKPLEIALACAASVIGTITTPQVTEQLTLESGEEYECEDEEEESETDQGEYSFSSAVCGMSQVAGAVAAVDLADDSGDNEDLADMYSASMGLLSVAQASTAIPLHCSIAEGTSVEAFRANVAEVLLREASAVFTHRQSYSSVANFLETTHNKIVDGITNPRRPYQEQQDVDNFTQEISDSIFQYALEKAEKRKELEGPGKDAPNIEGFLSDCVNNLLFDVLCVTSRKISDISNCNMESCDGQEGSVGYRAYEADSKAGREALSQLQHLIEPSQAYNHGERRSSVEKLSALIGKREREQKHIQEERENEARLKEPYRLALNRVTATMVKEQSDLQGQLGRSDKNADSALPSAESSPLHMQRGRAGGESETRQQSLSSSSGALVALKMDSGESKTPVTCFAEDLATTVVSMATELAAICLENSSGKQPWFCALNGGSEGPEGLLLPCRTAAALRRKETQNGTSVTKKHRPPRLSEIKRKTEEQPELMERLVNRVVDETVNLDEPTTTDPFALFASEVTARIMNCPELSVVDTSKSGQSSRSRLQCERWSSRGKASSYESIPEEDADPSGTSNTLGPGNRLGHNLSRGSSISKQSSCESITDEFSRFMVNQMETEGRGFDLLLDYYAGKNASSILAAAVQQAASKKNGHLNVRTSSCLSKQSSTESITEEFYRFMLKDMDKENKDYSMTKTKEWSNSLLPPSPRTPFCIRQSSVPDRRSSDSRLTVNSPIKANSFDGFAQNVHGDSLNIYPTNSVSSSGLCKSDSCLYKRGQTDQITDMLIHETWSSSIESLMRKNKIIAEPSEDSLELDSADSQPHVQQFANRLAADIVESGKSQLGGQQDVTAAACQPHIPVGERRHGFKHSRCNNSGNRPGVEHQENSCSSYSSSCVRQAWRGQREVPLIHIEPDQREEASEEKGGVETHHREASHQTQQQSGKGSETATKSRNGNSSTSSLGLADLEGFPDFSISSNLISEERERKTSHCQDQADEVTSGLSTAGSSCQRELLVLNCDLEPDCVDGELRAALQWISASELGVPALYFRKTHQHNLTKLHRVLQLAGQKAWRVGDLFSAVAQFCQLHQDLELKGRPLPSLFDWILKTKR

Target ORF information:

RefSeq Version	XM_009293859.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SPHK1 interactor, AKAP domain containing (sphkap), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293859.2

ORF Insert Sequence:

ATGCTAGCAA ACCTGCTCAG CGTCCTCCAA AACTTCACAG AGAGTAATTT CCAATCATCT 
GCCATGTTTG AGGGATCTGA GTCCGCGAAC ACACAGGAAG TCAAATCTGA GAGCACCCTT 
GGGTCCTCGC TCTCCGCCTG CAAGAAGGTT TTGTGCAGTA ACAGTGTTCT GGATTCGTCT 
GAGTACTGGC TGAAGAATGA TAAGACGCTC TGCAGAATTG GATTTCTGGA GGACCAGCAT 
GACAGCGGCT GCCCGACAAT TTGCTTTGTA AACCTGGACA GACATACTTC AGATTGGCAT 
GATGACAACT ACATCAAGAA GCTGGCCTCG GTGTGCCCAG AGCTGCCACG GTTAATTGAG 
TCTCTCGGAG TGCGGCAACC CAAAGAAAAT GAGATTCTGC TTCTCAGCAG CCTAGAGCCA 
CCGGACTCCT GCCAGCATGA CCCCAGCCAC CCACAGAGCC CGAGAACAGC GGATGTCTGT 
CTGGTGCATT GTTCATGTGG ACGCCGTCCC ACCCAAACGA GCAGTATCAT TTTTGAGATT 
AACAAGTTCC TGATTGGACT ACAGTCAGGG CAGGAGAGGC AGAAGCATGG CCGATTGGCT 
GGACAACGAG CTGCTGAAGA TGACACCAAT CGCTCTGTTT CGTCGATTGA AGAAGATTTC 
CTCACTGCCT CAGAGCAGCT TGGGGAAGAC AGCGAGGAAG ACCCTTTCAG GAATGATCCT 
GAGAATTCTC TCATGCCCGA GTCTGTTAAG GTGTTGAGAG CAGCCCACGC TCAAAAAAGG 
AGTGAAATAG AGAGAGAGGA CAGTGAGGAC TCTGAAACCC TCTGCACCTC TTCCAACTCC 
CAAAAGCTTT CAAGAACATA TTCCGCCCCT GCAAGAGGCC GACCCACCAC CCCAAAACAA 
GTCAAGGAGT CAGCCGGTCA CTACGCCACC AATCTGGCTG AGTCAGTCCT GCAAGATGCT 
TTTATTCGTT TGTCACAAGA TGAGCCATCA TTTGTTGCAG AAGCAGCAGT GAGTGTGTCA 
AGTGATATAA GCCATTCCAC TGAGGCACCA CAGAGGGCTC GAGCTTGCTC ATTCGAACTT 
CCTAAAATTG TGATTGTCCA AAGTCCTGAT AACAGTGAGG AGGTAACAGA ATGGCCGGAA 
GTGCAGTCAC ATGCAACATC AGAGCATGAT AGTGGAAATA AGGCCAAAGC AAAGCATGGT 
ACTCATCCCC CGCACAACTC GCCAGTTGGA CACCCTGCTA AGCCATTGGA AATAGCTTTG 
GCATGTGCTG CAAGTGTCAT TGGTACCATT ACCACCCCTC AAGTCACAGA ACAACTCACA 
CTAGAGTCAG GAGAGGAATA TGAGTGTGAA GATGAAGAGG AAGAAAGTGA AACTGATCAA 
GGGGAGTATT CGTTTTCATC TGCGGTATGT GGCATGTCTC AGGTCGCTGG AGCTGTAGCT 
GCGGTCGATC TAGCAGATGA CTCTGGGGAC AATGAAGACT TGGCAGACAT GTATTCCGCC 
TCTATGGGAC TTCTGTCAGT TGCCCAAGCT TCAACTGCAA TACCTCTTCA CTGTAGCATA 
GCAGAGGGCA CCAGCGTTGA GGCCTTTCGA GCCAATGTGG CTGAAGTTCT CCTCAGGGAA 
GCTTCAGCTG TATTCACTCA CAGACAAAGT TATTCAAGTG TTGCAAATTT CCTTGAGACC 
ACACATAACA AGATAGTGGA TGGCATCACA AATCCAAGAA GACCGTATCA GGAGCAACAA 
GATGTGGATA ACTTCACTCA AGAAATATCT GACAGTATCT TTCAGTATGC CCTTGAGAAG 
GCGGAGAAGA GAAAAGAACT TGAGGGTCCT GGAAAAGATG CCCCTAACAT TGAGGGTTTC 
CTCTCGGATT GTGTGAATAA TTTGCTCTTT GATGTACTGT GTGTAACATC AAGGAAAATA 
AGTGACATTT CAAATTGTAA TATGGAATCA TGTGATGGGC AGGAAGGCAG TGTCGGCTAT 
AGGGCATATG AGGCTGATTC CAAAGCTGGC AGGGAAGCAT TGAGCCAATT GCAGCATTTG 
ATTGAGCCAA GCCAGGCTTA TAATCATGGT GAAAGGCGAA GCAGTGTGGA GAAGTTATCT 
GCTCTAATAG GAAAGAGAGA GAGAGAGCAG AAACACATAC AGGAGGAAAG AGAAAATGAA 
GCCAGACTGA AGGAACCCTA CAGACTTGCT CTGAACAGAG TGACAGCCAC TATGGTCAAA 
GAGCAGTCTG ACCTGCAAGG GCAGCTGGGC AGGAGTGACA AGAATGCAGA CTCTGCGCTC 
CCTTCAGCAG AAAGCTCACC ACTTCACATG CAACGAGGGA GGGCAGGAGG AGAGAGCGAG 
ACCAGGCAGC AGTCTTTATC GTCCTCTTCT GGAGCACTTG TGGCACTTAA AATGGATTCG 
GGGGAGTCCA AAACTCCTGT CACGTGCTTT GCTGAAGATT TGGCGACCAC AGTGGTCTCC 
ATGGCTACAG AGCTGGCAGC GATATGCTTG GAGAACTCAA GTGGAAAGCA GCCGTGGTTC 
TGCGCATTAA ATGGTGGCTC GGAAGGTCCA GAAGGGCTGC TTCTGCCCTG CCGCACAGCT 
GCAGCTCTCC GCCGAAAGGA GACACAAAAT GGCACCTCGG TCACCAAGAA GCACAGACCG 
CCACGTCTCA GTGAAATCAA ACGTAAAACC GAAGAACAGC CCGAACTCAT GGAGCGCCTG 
GTCAACCGTG TTGTAGATGA AACCGTTAAC TTAGATGAAC CCACAACCAC TGATCCATTC 
GCGCTCTTTG CATCAGAGGT CACTGCCAGG ATAATGAACT GCCCAGAATT AAGTGTAGTG 
GACACTTCCA AATCTGGGCA GTCGTCCCGC AGCCGCCTGC AGTGTGAGAG ATGGAGTAGC 
AGGGGGAAGG CATCAAGCTA TGAGAGCATT CCAGAGGAGG ATGCAGATCC CTCCGGGACA 
TCAAATACTT TGGGTCCAGG AAACAGGCTT GGGCATAATT TGAGCCGAGG AAGCTCTATC 
TCCAAGCAGT CCAGCTGTGA GAGCATTACG GATGAGTTCT CTCGCTTCAT GGTGAACCAG 
ATGGAGACAG AGGGTCGAGG GTTTGACCTT CTGCTGGACT ACTATGCTGG GAAAAATGCC 
AGCAGCATCC TTGCAGCAGC TGTTCAGCAG GCTGCCAGTA AAAAAAACGG ACACCTCAAT 
GTGCGTACAT CATCTTGTCT CTCGAAGCAG TCCAGCACTG AGAGTATCAC AGAAGAGTTT 
TATCGCTTCA TGCTGAAAGA TATGGACAAG GAAAACAAGG ACTACAGTAT GACCAAGACC 
AAAGAGTGGA GTAATAGCCT GCTACCCCCG TCCCCCAGAA CCCCTTTTTG TATCCGTCAG 
TCTTCTGTTC CTGACAGGCG GTCTTCAGAT TCCAGACTAA CTGTAAACTC GCCTATCAAG 
GCCAATTCAT TCGATGGCTT TGCTCAAAAT GTACACGGGG ACTCGCTTAA CATCTATCCA 
ACTAACTCTG TGTCGTCATC TGGACTCTGC AAGTCCGATT CCTGCTTGTA TAAACGCGGC 
CAAACAGACC AAATCACAGA CATGCTGATT CATGAAACCT GGTCCAGCTC TATCGAATCT 
CTAATGCGCA AGAATAAGAT AATTGCCGAG CCCTCAGAGG ACAGTCTGGA ACTCGATTCT 
GCAGACTCAC AGCCACATGT ACAGCAGTTT GCTAATCGTC TAGCGGCAGA CATCGTAGAG 
AGTGGCAAAT CACAGCTTGG AGGCCAGCAG GATGTCACTG CGGCTGCTTG TCAGCCACAT 
ATTCCAGTTG GAGAAAGGAG ACACGGCTTT AAACATTCCC GTTGCAACAA CAGTGGAAAC 
CGGCCAGGCG TGGAGCATCA GGAAAACAGC TGTAGCTCTT ACAGTTCCTC CTGTGTGAGG 
CAGGCATGGC GCGGACAGAG AGAGGTACCC TTGATTCACA TTGAACCAGA TCAAAGAGAA 
GAGGCCTCGG AAGAGAAGGG AGGGGTTGAG ACCCACCACA GGGAGGCATC CCATCAGACC 
CAGCAACAAA GTGGCAAAGG GAGTGAGACA GCAACCAAAA GCAGAAATGG GAACAGCAGC 
ACTTCTAGTC TGGGACTGGC CGACCTAGAG GGCTTTCCGG ACTTCTCCAT CTCCAGCAAC 
CTAATCAGTG AGGAGAGGGA GAGGAAGACT TCGCACTGTC AGGACCAGGC GGACGAGGTG 
ACGTCGGGCT TGTCGACGGC CGGCAGCAGC TGTCAGCGGG AGCTCCTGGT GCTGAACTGT 
GACCTGGAGC CGGACTGTGT GGACGGAGAG CTGAGAGCCG CGCTGCAGTG GATCTCAGCA 
TCTGAACTCG GCGTCCCGGC GCTTTACTTC AGAAAGACTC ACCAGCACAA TCTTACAAAG 
CTCCACAGAG TGCTGCAGTT GGCAGGTCAG AAGGCTTGGC GTGTTGGAGA CTTGTTCAGC 
GCCGTGGCTC AGTTCTGTCA GCTCCACCAG GACCTGGAGC TGAAAGGACG GCCGCTGCCC 
AGCCTGTTTG ACTGGATCCT GAAGACCAAA CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Dner inhibits neural progenitor proliferation and induces neuronal and glial differentiation in zebrafish.
Developmental biology375(1)1-12(2013 Mar)
Hsieh FY,Ma TL,Shih HY,Lin SJ,Huang CW,Wang HY,Cheng YC

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sphkap cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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