SPHKAP gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015276894.1	XP_015132380.1	A-kinase anchor protein SPHKAP isoform X6
XM_015276888.2	XP_015132374.1	A-kinase anchor protein SPHKAP isoform X1
XM_015276888.1	XP_015132374.1	A-kinase anchor protein SPHKAP isoform X1
XM_015276889.1	XP_015132375.1	A-kinase anchor protein SPHKAP isoform X2
XM_015276892.1	XP_015132378.1	A-kinase anchor protein SPHKAP isoform X5
XM_015276893.1	XP_015132379.1	A-kinase anchor protein SPHKAP isoform X5
XM_015276893.2	XP_015132379.1	A-kinase anchor protein SPHKAP isoform X5
XM_015276891.1	XP_015132377.1	A-kinase anchor protein SPHKAP isoform X4
NM_001177532.3	NP_001171003.1	A-kinase anchor protein SPHKAP
XM_015276894.2	XP_015132380.1	A-kinase anchor protein SPHKAP isoform X6
XM_015276889.2	XP_015132375.1	A-kinase anchor protein SPHKAP isoform X2
XM_015276890.1	XP_015132376.1	A-kinase anchor protein SPHKAP isoform X3
XM_015276892.2	XP_015132378.1	A-kinase anchor protein SPHKAP isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa39662	XM_015276888.2 Latest version!	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa39663	XM_015276889.2 Latest version!	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa39664	XM_015276890.1 Latest version!	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa39665	XM_015276891.1 Latest version!	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa39666	XM_015276893.2 Latest version!	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa39666	XM_015276892.2 Latest version!	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa39667	XM_015276894.2 Latest version!	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa24472	NM_001177532.3 Latest version!	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OGa39662	XM_015276888.1	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa39666	XM_015276893.1	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa39667	XM_015276894.1	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa39663	XM_015276889.1	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa39666	XM_015276892.1	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

SPHKAP ( XM_015276888.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132374.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa39662
Clone ID Related Accession (Same CDS sequence)	XM_015276888.2 , XM_015276888.1
Accession Version	XM_015276888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276888.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCC CGGCCACCCG CCGCCACCCG GCGCTTCGCA GCAACTTTGA ATCTCCACTG 
ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG CAATGGCAAC 
AGCAGTACAA CTAGCAGTCT TGGCATTATG GACCTGGAAA TTTATCAGGA GAACGTGCCA 
CCTTCTCCTA TGATTAATGA CTTAGTAGAA GAAAAGGTTT TCCTTAAAGA ACAGACAGAT 
AATACAGAGG AAAGTACTTC TGGGCTCTCA GTGGGAACAG CCAATTATCA GAAAGAACTC 
CTGGTGATTA ATTTTGATCT GGAACCAGAA TGCCCCAACG CAGAGCTGCG AGCCACCCTA 
CAGTGGATAG CTGCTTCTGA ACTTGGAATC CCAACCATCT ATTTTAAAAA ATCTCAGGAA 
AACAGAATTG AAAAGTTTTT AGATGTTGTG CAGCTAGTTC AACGGAAGTC CTGGAAAGTG 
GGTGACATCT TTCATGCTGT GGTGCAGTAC TGCAAACTCA GTGAGGAGCG CAAAGAGAAG 
ACACCAAGCC TGTTCGATTG GCTCCTTGAA CTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132374.1
CDS	263..5341
Translation	MFRPATRRHPALRSNFESPLMQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTVAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENVPPSPMINDLVEEKVFLKEQTDNTEESTSGLSVGTANYQKELLVINFDLEPECPNAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFHAVVQYCKLSEERKEKTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_015276888.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276888.2

ORF Insert Sequence:

ATGTTCCGCC CGGCCACCCG CCGCCACCCG GCGCTTCGCA GCAACTTTGA ATCTCCACTG 
ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG CAATGGCAAC 
AGCAGTACAA CTAGCAGTCT TGGCATTATG GACCTGGAAA TTTATCAGGA GAACGTGCCA 
CCTTCTCCTA TGATTAATGA CTTAGTAGAA GAAAAGGTTT TCCTTAAAGA ACAGACAGAT 
AATACAGAGG AAAGTACTTC TGGGCTCTCA GTGGGAACAG CCAATTATCA GAAAGAACTC 
CTGGTGATTA ATTTTGATCT GGAACCAGAA TGCCCCAACG CAGAGCTGCG AGCCACCCTA 
CAGTGGATAG CTGCTTCTGA ACTTGGAATC CCAACCATCT ATTTTAAAAA ATCTCAGGAA 
AACAGAATTG AAAAGTTTTT AGATGTTGTG CAGCTAGTTC AACGGAAGTC CTGGAAAGTG 
GGTGACATCT TTCATGCTGT GGTGCAGTAC TGCAAACTCA GTGAGGAGCG CAAAGAGAAG 
ACACCAAGCC TGTTCGATTG GCTCCTTGAA CTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_015276888.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132374.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

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CloneID	OGa39662
Clone ID Related Accession (Same CDS sequence)	XM_015276888.2 , XM_015276888.1
Accession Version	XM_015276888.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCC CGGCCACCCG CCGCCACCCG GCGCTTCGCA GCAACTTTGA ATCTCCACTG 
ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG CAATGGCAAC 
AGCAGTACAA CTAGCAGTCT TGGCATTATG GACCTGGAAA TTTATCAGGA GAACGTGCCA 
CCTTCTCCTA TGATTAATGA CTTAGTAGAA GAAAAGGTTT TCCTTAAAGA ACAGACAGAT 
AATACAGAGG AAAGTACTTC TGGGCTCTCA GTGGGAACAG CCAATTATCA GAAAGAACTC 
CTGGTGATTA ATTTTGATCT GGAACCAGAA TGCCCCAACG CAGAGCTGCG AGCCACCCTA 
CAGTGGATAG CTGCTTCTGA ACTTGGAATC CCAACCATCT ATTTTAAAAA ATCTCAGGAA 
AACAGAATTG AAAAGTTTTT AGATGTTGTG CAGCTAGTTC AACGGAAGTC CTGGAAAGTG 
GGTGACATCT TTCATGCTGT GGTGCAGTAC TGCAAACTCA GTGAGGAGCG CAAAGAGAAG 
ACACCAAGCC TGTTCGATTG GCTCCTTGAA CTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132374.1
CDS	577..5655
Translation	MFRPATRRHPALRSNFESPLMQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTVAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENVPPSPMINDLVEEKVFLKEQTDNTEESTSGLSVGTANYQKELLVINFDLEPECPNAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFHAVVQYCKLSEERKEKTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_015276888.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276888.1

ORF Insert Sequence:

ATGTTCCGCC CGGCCACCCG CCGCCACCCG GCGCTTCGCA GCAACTTTGA ATCTCCACTG 
ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG CAATGGCAAC 
AGCAGTACAA CTAGCAGTCT TGGCATTATG GACCTGGAAA TTTATCAGGA GAACGTGCCA 
CCTTCTCCTA TGATTAATGA CTTAGTAGAA GAAAAGGTTT TCCTTAAAGA ACAGACAGAT 
AATACAGAGG AAAGTACTTC TGGGCTCTCA GTGGGAACAG CCAATTATCA GAAAGAACTC 
CTGGTGATTA ATTTTGATCT GGAACCAGAA TGCCCCAACG CAGAGCTGCG AGCCACCCTA 
CAGTGGATAG CTGCTTCTGA ACTTGGAATC CCAACCATCT ATTTTAAAAA ATCTCAGGAA 
AACAGAATTG AAAAGTTTTT AGATGTTGTG CAGCTAGTTC AACGGAAGTC CTGGAAAGTG 
GGTGACATCT TTCATGCTGT GGTGCAGTAC TGCAAACTCA GTGAGGAGCG CAAAGAGAAG 
ACACCAAGCC TGTTCGATTG GCTCCTTGAA CTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_015276889.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132375.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa39663
Clone ID Related Accession (Same CDS sequence)	XM_015276889.1 , XM_015276889.2
Accession Version	XM_015276889.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCC CGGCCACCCG CCGCCACCCG GCGCTTCGCA GCAACTTTGA ATCTCCACTG 
ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGCCCA CGAAAAAATA 
AGTGTTTCAG AAGCATCTTC TGATGTCAGA AAAAATAAAG GAAAAGGATT TGAAAGTCTC 
CACTACAGAA AAGCCAGGTT GCCATTAATT CTTGAAGGGA ATTGTGTTAA TGAAGATAAT 
ACAGCTAGTA AAGTCTCTGA ATCTGTGCAC GTTGTGGCTG AAGATGGCAT CTCACAACCT 
GAAGATAAGC CAGAGCAGGA AATCCTGTGT CAAGAGGAAT TAGTGGGAAG AGATACATCA 
TCCCTCAGTA CTGAGGATTT GACTAACAAT GTTGAAAATT CCTGCTCAGT GAATGTGTTA 
GATGTGTCTT TAACCAAAAT GGCTAAAGAG GAGCTGGGGC CTCTTTATGG CTCTACAGCA 
AAACCCAACA GTAAGGCAGA GGTAGAGGAT TGTGCAGGTG TTAGGAAAAG CGACCCTCCC 
TTGCAAAACG AGCAAGCAGC TACAGGTCAG TACGCTACAA ATCTAGCAGA ATCTGTTCTG 
CAAGATGCAT TCATCAGACT ATCACAGTCT CAACCCACTT TCAGTGAGGA GGCTGCAGTC 
AGCATCTCCG TAGGAAGCTC CTGCAAGTCA AAAGATGCAT CTGCTTCCCG ATCGTGGAAT 
GAACTTCCAA AGATTGTCAT AGTGCAAAGT CCAGACAGCA GTGAGAATGT GCCAGACTGG 
CCAGGGTCTG CCTTCCCAAA CCTGTGCCAC TGGACTGAAA CAGAAAGTTC TGAAGTTTCA 
GATTACTTTG AAGAAGACCA TTCAAATGGA CGCAGCCAGA GTGCACTGGA GATGGCTCTG 
GCTTGTGCAG CAACTGTCAT TGGAACCATT TCAAGTCCAC AGGCAGCAGA AAAATTCAGA 
AGGGATCAAG AAGCCACAGA CTCTAAAAGC GAAACAGTTG ATAACGAAGA TCTACACGCA 
GATTCTTCAC AGCTACTTGA AGACTGTACT AGCACAGATT ATTCATTTCC ATCTGCACTG 
TGTGGCATGA CTCAAGTAGC AAGTGCTGTA GCAGTCTGCG GTCTGGGGGA AACAAAGGAA 
GAGAAGTACC CTACAACTTC AAGCGGGCTG CTGTCTGCAG CTCAGACTTC TGCAGCCATT 
ACTCTTCATT GTAGCATAGC TGTAGGAAGC AGCATGGAAA AGCTGAATGA TAGCATTGCA 
GAGGCCCTAC TCAAAGAGGC ATCAATAATT TTGACAAAAC CGAATACATA CAAAAATGTA 
GGGCATTTTA TGGAATCCAT AAACGGAAGA ATTATTGAAA CAGCAGCAAG GCCACGGATT 
CCACACTCCG ATGAAGTAAT CAGAGATGAA CTGGCACAAA ATTTATCCAA TATTATTCTA 
CGACATTCTA TTGAAGAGGT CAGGAAGAAA AGAGAGCTAC AGTCCTGTTC AGAAAACAGC 
TCAAGTACAC AAGACATTTT CATGGAGACT GCCAATGAGT TGCTTTTTAA TGTGATATAT 
TTCACTTGCA AGAAGATGAA TGACATAAGA CAAGTTGAAG AGTGTTCTCC TCTCTTTTCT 
GAAGGCTTGA AAGGCAAGAA GGTAACAAGA GTAGAAGGAT GGCCAACACA GGCAACAGCA 
TGTGAAACAT CACACAGACC CCTTGATCAT TCTGCTATTA AGCCATTTGG TACATCCTAC 
AGTGCTAGCA CAGATAGTGA AAATGGTATC AGCACGAAGA AAATTAACAT GAGAGATGTA 
AATAGCAATG AAAACGCCAC AGTGAATTCA GAGCTAACAA GTAGAGTCAT TGGGCATAAT 
GCACTTGTTG CAAAAACATC TCCCAAGAAA AGATACCTGA AAAGAACCAC ACGAGACTGC 
TACAAATCCC CACATCAGAA TAACATTCAG AAGAAGAGAG ATTACAGATC ATTTTCAGAC 
AGAGAAAGTA CCTTTGCAAA CAATGAATGC AGGCACGGTG TTCAAGAACA ACTGTCTTCC 
AGCGCTGTCG TGAATGCAGA AAACCAGGCC AAACATAAGT GTGATGCTGT GCTAAGTGAT 
GAAGTCCAAG TTAGCTTGTC TTTGTTAGGA AACCATGTCT TACTTCCTTC TCAGCCTGTG 
CTACAGGTGA AAAATTCAAG AGACAAATAC TGTATAACAG ATTTTGCAGA AGAATTGGCA 
GAAACAGTGG TCTCTATGGC AACAGAAATA GCTGCCATTT GTCTTGAAAA CTCAAATGGA 
AAGCAGCCCT GGTTCTGTGC ATGGAAGAGA GGCAATGAAT ATCTGGTGAC CCAGAGTATA 
TCATGCAGAA CTATGAAAAG GAAAAAGGAA ACCCATGCTA ATGGCTCAGT TGTTCGGAAA 
CACAGGGCAC CTCGACTGAG TGAGATCAAA AGAAAGACAG ATGAGCATCC TGAGCTAAAG 
GAAAGGTTGA TGAATCGAGT AGTAGATGAA TCTATAAACC TTGAGGACAC ACCAGATTCT 
GTCAATATAT TTGCAAATGA AGTGGCTGCA AAGATCATGA ACCTCACTGA ACTCTCCATG 
GTTGACAGTA TCTGGCAAGG TCCAAACCAT CCCAGGAACA GGCTGCACTG TGAAAGATGG 
AGCCGAGCCA AGGCCTCAAG CTGTGAGAGC ATACCAGAGG AGGACATGGA TTCCAAAGCC 
TCTGCCAATA CTCTAGGCCT AATGAATGCC CTTGGTCAGC CTGTGAGCCA GACAAGTTCC 
GTCTCAAAGC AGTCTAGTTG TGAAAGCATT ACAGATGAGT TTTCAAGATT TATGGTGAAC 
CAAATGGAAA ATGAGGGAAG AGGCTTTGAT TTATTACTAG ATTATTATGC AGGAAAAAAT 
GCAAACAACA TCTTAACGTC GGCTTTGCAA CAGGTTGCTA AGAAAAATGG TCATCTTAAT 
GTACGGCCAA GTTGCCCATC CAAGCAGTCC AGCACGGAAA GCATAACAGA AGAATTTTAC 
AGGTATATGC TAAAAGAAAT AGAAAAGGAA AATAAAGATA ATGCATCTTC CCCTCGGAAT 
TCAAAGGACT GGTGTGGCAG TTTGCTACCA CCCTCTCTGC GATCACCTTT TTGCTTTAGA 
CAGTCCTCAG TGCCTGATGG CAGATCATCA GGTTCTAGAC TAACAGTTAA CATCCCAGTC 
AAGGCAAATT CATTAGATGG ATTTGCCCAA CATCACCAGG ATTCCTTAAG TGTACAGCCA 
GTCAGTACTG TGGCTTCTTC AGGTCTTTGC AAGTCTGACT CATGCCTGTA CCAAAGATGC 
AAGACTGACC AGATAACAGA TATGTTGATT CACGAGACGT GGGCAAGCTC TATTGAATCT 
CTAATGCGCA AAAACAAAAT CATAACAGAT GAGGCAGAGG TTGCAGAGGT AGATCATTTT 
CACAGTGATT CCCCACCACA TGTGCAACAG TATGCAAACA GACTGGCTGC AAATATTGTT 
GAAAGTGGTA AAAATTTAAT TGTTGTCCAG CAGGATGCCT TTGATTCTGC AAGCCAAGAA 
CACATGCTGG AAAGCAAACA TCCCCAGAGT GCAACTCAGA TTCAAGTAGA ACCCAAAAGG 
AGTGGAATAA ATTTGGATGA GCATATGAAG AACCCTGAAT GCCTCCCTGC AGGTCAGCAC 
AGGGAAGTGC CTTTAATTCA GATAGAAACT GATCAACGAG ACGAGTCAGA TAAAGACTCT 
GAATCCTTAA CTTTACATGA GCTTTCTGAA AAGGAGCATC AAGGCAAGGA AAAGCCTCCA 
GAAGCTTATG CTGGGAAGCA CATGGTTTCC AGTACCCTAC TGAATAGCAA AAGCAGTCAG 
AGCAAACCAG ATGCTGAAAT AACGGGAGAG ACAAAAGCAG CTGAAGAATT TCCAACACAC 
CTCAGCAGCA GTGAAGAAAG CACTGGCAGC TGGTCCCAGC TAGCCAATGA TGAAGACAAT 
CCTGATGACA CAAGTAGCTA CTTACAACTC AGCGAACGAT CTCTGAGCAA TGGCAACAGC 
AGTACAACTA GCAGTCTTGG CATTATGGAC CTGGAAATTT ATCAGGAGAA CGTGCCACCT 
TCTCCTATGA TTAATGACTT AGTAGAAGAA AAGGTTTTCC TTAAAGAACA GACAGATAAT 
ACAGAGGAAA GTACTTCTGG GCTCTCAGTG GGAACAGCCA ATTATCAGAA AGAACTCCTG 
GTGATTAATT TTGATCTGGA ACCAGAATGC CCCAACGCAG AGCTGCGAGC CACCCTACAG 
TGGATAGCTG CTTCTGAACT TGGAATCCCA ACCATCTATT TTAAAAAATC TCAGGAAAAC 
AGAATTGAAA AGTTTTTAGA TGTTGTGCAG CTAGTTCAAC GGAAGTCCTG GAAAGTGGGT 
GACATCTTTC ATGCTGTGGT GCAGTACTGC AAACTCAGTG AGGAGCGCAA AGAGAAGACA 
CCAAGCCTGT TCGATTGGCT CCTTGAACTG GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132375.1
CDS	577..5652
Translation	MFRPATRRHPALRSNFESPLMQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENVPPSPMINDLVEEKVFLKEQTDNTEESTSGLSVGTANYQKELLVINFDLEPECPNAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFHAVVQYCKLSEERKEKTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_015276889.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276889.1

ORF Insert Sequence:

ATGTTCCGCC CGGCCACCCG CCGCCACCCG GCGCTTCGCA GCAACTTTGA ATCTCCACTG 
ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGCCCA CGAAAAAATA 
AGTGTTTCAG AAGCATCTTC TGATGTCAGA AAAAATAAAG GAAAAGGATT TGAAAGTCTC 
CACTACAGAA AAGCCAGGTT GCCATTAATT CTTGAAGGGA ATTGTGTTAA TGAAGATAAT 
ACAGCTAGTA AAGTCTCTGA ATCTGTGCAC GTTGTGGCTG AAGATGGCAT CTCACAACCT 
GAAGATAAGC CAGAGCAGGA AATCCTGTGT CAAGAGGAAT TAGTGGGAAG AGATACATCA 
TCCCTCAGTA CTGAGGATTT GACTAACAAT GTTGAAAATT CCTGCTCAGT GAATGTGTTA 
GATGTGTCTT TAACCAAAAT GGCTAAAGAG GAGCTGGGGC CTCTTTATGG CTCTACAGCA 
AAACCCAACA GTAAGGCAGA GGTAGAGGAT TGTGCAGGTG TTAGGAAAAG CGACCCTCCC 
TTGCAAAACG AGCAAGCAGC TACAGGTCAG TACGCTACAA ATCTAGCAGA ATCTGTTCTG 
CAAGATGCAT TCATCAGACT ATCACAGTCT CAACCCACTT TCAGTGAGGA GGCTGCAGTC 
AGCATCTCCG TAGGAAGCTC CTGCAAGTCA AAAGATGCAT CTGCTTCCCG ATCGTGGAAT 
GAACTTCCAA AGATTGTCAT AGTGCAAAGT CCAGACAGCA GTGAGAATGT GCCAGACTGG 
CCAGGGTCTG CCTTCCCAAA CCTGTGCCAC TGGACTGAAA CAGAAAGTTC TGAAGTTTCA 
GATTACTTTG AAGAAGACCA TTCAAATGGA CGCAGCCAGA GTGCACTGGA GATGGCTCTG 
GCTTGTGCAG CAACTGTCAT TGGAACCATT TCAAGTCCAC AGGCAGCAGA AAAATTCAGA 
AGGGATCAAG AAGCCACAGA CTCTAAAAGC GAAACAGTTG ATAACGAAGA TCTACACGCA 
GATTCTTCAC AGCTACTTGA AGACTGTACT AGCACAGATT ATTCATTTCC ATCTGCACTG 
TGTGGCATGA CTCAAGTAGC AAGTGCTGTA GCAGTCTGCG GTCTGGGGGA AACAAAGGAA 
GAGAAGTACC CTACAACTTC AAGCGGGCTG CTGTCTGCAG CTCAGACTTC TGCAGCCATT 
ACTCTTCATT GTAGCATAGC TGTAGGAAGC AGCATGGAAA AGCTGAATGA TAGCATTGCA 
GAGGCCCTAC TCAAAGAGGC ATCAATAATT TTGACAAAAC CGAATACATA CAAAAATGTA 
GGGCATTTTA TGGAATCCAT AAACGGAAGA ATTATTGAAA CAGCAGCAAG GCCACGGATT 
CCACACTCCG ATGAAGTAAT CAGAGATGAA CTGGCACAAA ATTTATCCAA TATTATTCTA 
CGACATTCTA TTGAAGAGGT CAGGAAGAAA AGAGAGCTAC AGTCCTGTTC AGAAAACAGC 
TCAAGTACAC AAGACATTTT CATGGAGACT GCCAATGAGT TGCTTTTTAA TGTGATATAT 
TTCACTTGCA AGAAGATGAA TGACATAAGA CAAGTTGAAG AGTGTTCTCC TCTCTTTTCT 
GAAGGCTTGA AAGGCAAGAA GGTAACAAGA GTAGAAGGAT GGCCAACACA GGCAACAGCA 
TGTGAAACAT CACACAGACC CCTTGATCAT TCTGCTATTA AGCCATTTGG TACATCCTAC 
AGTGCTAGCA CAGATAGTGA AAATGGTATC AGCACGAAGA AAATTAACAT GAGAGATGTA 
AATAGCAATG AAAACGCCAC AGTGAATTCA GAGCTAACAA GTAGAGTCAT TGGGCATAAT 
GCACTTGTTG CAAAAACATC TCCCAAGAAA AGATACCTGA AAAGAACCAC ACGAGACTGC 
TACAAATCCC CACATCAGAA TAACATTCAG AAGAAGAGAG ATTACAGATC ATTTTCAGAC 
AGAGAAAGTA CCTTTGCAAA CAATGAATGC AGGCACGGTG TTCAAGAACA ACTGTCTTCC 
AGCGCTGTCG TGAATGCAGA AAACCAGGCC AAACATAAGT GTGATGCTGT GCTAAGTGAT 
GAAGTCCAAG TTAGCTTGTC TTTGTTAGGA AACCATGTCT TACTTCCTTC TCAGCCTGTG 
CTACAGGTGA AAAATTCAAG AGACAAATAC TGTATAACAG ATTTTGCAGA AGAATTGGCA 
GAAACAGTGG TCTCTATGGC AACAGAAATA GCTGCCATTT GTCTTGAAAA CTCAAATGGA 
AAGCAGCCCT GGTTCTGTGC ATGGAAGAGA GGCAATGAAT ATCTGGTGAC CCAGAGTATA 
TCATGCAGAA CTATGAAAAG GAAAAAGGAA ACCCATGCTA ATGGCTCAGT TGTTCGGAAA 
CACAGGGCAC CTCGACTGAG TGAGATCAAA AGAAAGACAG ATGAGCATCC TGAGCTAAAG 
GAAAGGTTGA TGAATCGAGT AGTAGATGAA TCTATAAACC TTGAGGACAC ACCAGATTCT 
GTCAATATAT TTGCAAATGA AGTGGCTGCA AAGATCATGA ACCTCACTGA ACTCTCCATG 
GTTGACAGTA TCTGGCAAGG TCCAAACCAT CCCAGGAACA GGCTGCACTG TGAAAGATGG 
AGCCGAGCCA AGGCCTCAAG CTGTGAGAGC ATACCAGAGG AGGACATGGA TTCCAAAGCC 
TCTGCCAATA CTCTAGGCCT AATGAATGCC CTTGGTCAGC CTGTGAGCCA GACAAGTTCC 
GTCTCAAAGC AGTCTAGTTG TGAAAGCATT ACAGATGAGT TTTCAAGATT TATGGTGAAC 
CAAATGGAAA ATGAGGGAAG AGGCTTTGAT TTATTACTAG ATTATTATGC AGGAAAAAAT 
GCAAACAACA TCTTAACGTC GGCTTTGCAA CAGGTTGCTA AGAAAAATGG TCATCTTAAT 
GTACGGCCAA GTTGCCCATC CAAGCAGTCC AGCACGGAAA GCATAACAGA AGAATTTTAC 
AGGTATATGC TAAAAGAAAT AGAAAAGGAA AATAAAGATA ATGCATCTTC CCCTCGGAAT 
TCAAAGGACT GGTGTGGCAG TTTGCTACCA CCCTCTCTGC GATCACCTTT TTGCTTTAGA 
CAGTCCTCAG TGCCTGATGG CAGATCATCA GGTTCTAGAC TAACAGTTAA CATCCCAGTC 
AAGGCAAATT CATTAGATGG ATTTGCCCAA CATCACCAGG ATTCCTTAAG TGTACAGCCA 
GTCAGTACTG TGGCTTCTTC AGGTCTTTGC AAGTCTGACT CATGCCTGTA CCAAAGATGC 
AAGACTGACC AGATAACAGA TATGTTGATT CACGAGACGT GGGCAAGCTC TATTGAATCT 
CTAATGCGCA AAAACAAAAT CATAACAGAT GAGGCAGAGG TTGCAGAGGT AGATCATTTT 
CACAGTGATT CCCCACCACA TGTGCAACAG TATGCAAACA GACTGGCTGC AAATATTGTT 
GAAAGTGGTA AAAATTTAAT TGTTGTCCAG CAGGATGCCT TTGATTCTGC AAGCCAAGAA 
CACATGCTGG AAAGCAAACA TCCCCAGAGT GCAACTCAGA TTCAAGTAGA ACCCAAAAGG 
AGTGGAATAA ATTTGGATGA GCATATGAAG AACCCTGAAT GCCTCCCTGC AGGTCAGCAC 
AGGGAAGTGC CTTTAATTCA GATAGAAACT GATCAACGAG ACGAGTCAGA TAAAGACTCT 
GAATCCTTAA CTTTACATGA GCTTTCTGAA AAGGAGCATC AAGGCAAGGA AAAGCCTCCA 
GAAGCTTATG CTGGGAAGCA CATGGTTTCC AGTACCCTAC TGAATAGCAA AAGCAGTCAG 
AGCAAACCAG ATGCTGAAAT AACGGGAGAG ACAAAAGCAG CTGAAGAATT TCCAACACAC 
CTCAGCAGCA GTGAAGAAAG CACTGGCAGC TGGTCCCAGC TAGCCAATGA TGAAGACAAT 
CCTGATGACA CAAGTAGCTA CTTACAACTC AGCGAACGAT CTCTGAGCAA TGGCAACAGC 
AGTACAACTA GCAGTCTTGG CATTATGGAC CTGGAAATTT ATCAGGAGAA CGTGCCACCT 
TCTCCTATGA TTAATGACTT AGTAGAAGAA AAGGTTTTCC TTAAAGAACA GACAGATAAT 
ACAGAGGAAA GTACTTCTGG GCTCTCAGTG GGAACAGCCA ATTATCAGAA AGAACTCCTG 
GTGATTAATT TTGATCTGGA ACCAGAATGC CCCAACGCAG AGCTGCGAGC CACCCTACAG 
TGGATAGCTG CTTCTGAACT TGGAATCCCA ACCATCTATT TTAAAAAATC TCAGGAAAAC 
AGAATTGAAA AGTTTTTAGA TGTTGTGCAG CTAGTTCAAC GGAAGTCCTG GAAAGTGGGT 
GACATCTTTC ATGCTGTGGT GCAGTACTGC AAACTCAGTG AGGAGCGCAA AGAGAAGACA 
CCAAGCCTGT TCGATTGGCT CCTTGAACTG GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_015276889.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132375.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

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CloneID	OGa39663
Clone ID Related Accession (Same CDS sequence)	XM_015276889.1 , XM_015276889.2
Accession Version	XM_015276889.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCC CGGCCACCCG CCGCCACCCG GCGCTTCGCA GCAACTTTGA ATCTCCACTG 
ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGCCCA CGAAAAAATA 
AGTGTTTCAG AAGCATCTTC TGATGTCAGA AAAAATAAAG GAAAAGGATT TGAAAGTCTC 
CACTACAGAA AAGCCAGGTT GCCATTAATT CTTGAAGGGA ATTGTGTTAA TGAAGATAAT 
ACAGCTAGTA AAGTCTCTGA ATCTGTGCAC GTTGTGGCTG AAGATGGCAT CTCACAACCT 
GAAGATAAGC CAGAGCAGGA AATCCTGTGT CAAGAGGAAT TAGTGGGAAG AGATACATCA 
TCCCTCAGTA CTGAGGATTT GACTAACAAT GTTGAAAATT CCTGCTCAGT GAATGTGTTA 
GATGTGTCTT TAACCAAAAT GGCTAAAGAG GAGCTGGGGC CTCTTTATGG CTCTACAGCA 
AAACCCAACA GTAAGGCAGA GGTAGAGGAT TGTGCAGGTG TTAGGAAAAG CGACCCTCCC 
TTGCAAAACG AGCAAGCAGC TACAGGTCAG TACGCTACAA ATCTAGCAGA ATCTGTTCTG 
CAAGATGCAT TCATCAGACT ATCACAGTCT CAACCCACTT TCAGTGAGGA GGCTGCAGTC 
AGCATCTCCG TAGGAAGCTC CTGCAAGTCA AAAGATGCAT CTGCTTCCCG ATCGTGGAAT 
GAACTTCCAA AGATTGTCAT AGTGCAAAGT CCAGACAGCA GTGAGAATGT GCCAGACTGG 
CCAGGGTCTG CCTTCCCAAA CCTGTGCCAC TGGACTGAAA CAGAAAGTTC TGAAGTTTCA 
GATTACTTTG AAGAAGACCA TTCAAATGGA CGCAGCCAGA GTGCACTGGA GATGGCTCTG 
GCTTGTGCAG CAACTGTCAT TGGAACCATT TCAAGTCCAC AGGCAGCAGA AAAATTCAGA 
AGGGATCAAG AAGCCACAGA CTCTAAAAGC GAAACAGTTG ATAACGAAGA TCTACACGCA 
GATTCTTCAC AGCTACTTGA AGACTGTACT AGCACAGATT ATTCATTTCC ATCTGCACTG 
TGTGGCATGA CTCAAGTAGC AAGTGCTGTA GCAGTCTGCG GTCTGGGGGA AACAAAGGAA 
GAGAAGTACC CTACAACTTC AAGCGGGCTG CTGTCTGCAG CTCAGACTTC TGCAGCCATT 
ACTCTTCATT GTAGCATAGC TGTAGGAAGC AGCATGGAAA AGCTGAATGA TAGCATTGCA 
GAGGCCCTAC TCAAAGAGGC ATCAATAATT TTGACAAAAC CGAATACATA CAAAAATGTA 
GGGCATTTTA TGGAATCCAT AAACGGAAGA ATTATTGAAA CAGCAGCAAG GCCACGGATT 
CCACACTCCG ATGAAGTAAT CAGAGATGAA CTGGCACAAA ATTTATCCAA TATTATTCTA 
CGACATTCTA TTGAAGAGGT CAGGAAGAAA AGAGAGCTAC AGTCCTGTTC AGAAAACAGC 
TCAAGTACAC AAGACATTTT CATGGAGACT GCCAATGAGT TGCTTTTTAA TGTGATATAT 
TTCACTTGCA AGAAGATGAA TGACATAAGA CAAGTTGAAG AGTGTTCTCC TCTCTTTTCT 
GAAGGCTTGA AAGGCAAGAA GGTAACAAGA GTAGAAGGAT GGCCAACACA GGCAACAGCA 
TGTGAAACAT CACACAGACC CCTTGATCAT TCTGCTATTA AGCCATTTGG TACATCCTAC 
AGTGCTAGCA CAGATAGTGA AAATGGTATC AGCACGAAGA AAATTAACAT GAGAGATGTA 
AATAGCAATG AAAACGCCAC AGTGAATTCA GAGCTAACAA GTAGAGTCAT TGGGCATAAT 
GCACTTGTTG CAAAAACATC TCCCAAGAAA AGATACCTGA AAAGAACCAC ACGAGACTGC 
TACAAATCCC CACATCAGAA TAACATTCAG AAGAAGAGAG ATTACAGATC ATTTTCAGAC 
AGAGAAAGTA CCTTTGCAAA CAATGAATGC AGGCACGGTG TTCAAGAACA ACTGTCTTCC 
AGCGCTGTCG TGAATGCAGA AAACCAGGCC AAACATAAGT GTGATGCTGT GCTAAGTGAT 
GAAGTCCAAG TTAGCTTGTC TTTGTTAGGA AACCATGTCT TACTTCCTTC TCAGCCTGTG 
CTACAGGTGA AAAATTCAAG AGACAAATAC TGTATAACAG ATTTTGCAGA AGAATTGGCA 
GAAACAGTGG TCTCTATGGC AACAGAAATA GCTGCCATTT GTCTTGAAAA CTCAAATGGA 
AAGCAGCCCT GGTTCTGTGC ATGGAAGAGA GGCAATGAAT ATCTGGTGAC CCAGAGTATA 
TCATGCAGAA CTATGAAAAG GAAAAAGGAA ACCCATGCTA ATGGCTCAGT TGTTCGGAAA 
CACAGGGCAC CTCGACTGAG TGAGATCAAA AGAAAGACAG ATGAGCATCC TGAGCTAAAG 
GAAAGGTTGA TGAATCGAGT AGTAGATGAA TCTATAAACC TTGAGGACAC ACCAGATTCT 
GTCAATATAT TTGCAAATGA AGTGGCTGCA AAGATCATGA ACCTCACTGA ACTCTCCATG 
GTTGACAGTA TCTGGCAAGG TCCAAACCAT CCCAGGAACA GGCTGCACTG TGAAAGATGG 
AGCCGAGCCA AGGCCTCAAG CTGTGAGAGC ATACCAGAGG AGGACATGGA TTCCAAAGCC 
TCTGCCAATA CTCTAGGCCT AATGAATGCC CTTGGTCAGC CTGTGAGCCA GACAAGTTCC 
GTCTCAAAGC AGTCTAGTTG TGAAAGCATT ACAGATGAGT TTTCAAGATT TATGGTGAAC 
CAAATGGAAA ATGAGGGAAG AGGCTTTGAT TTATTACTAG ATTATTATGC AGGAAAAAAT 
GCAAACAACA TCTTAACGTC GGCTTTGCAA CAGGTTGCTA AGAAAAATGG TCATCTTAAT 
GTACGGCCAA GTTGCCCATC CAAGCAGTCC AGCACGGAAA GCATAACAGA AGAATTTTAC 
AGGTATATGC TAAAAGAAAT AGAAAAGGAA AATAAAGATA ATGCATCTTC CCCTCGGAAT 
TCAAAGGACT GGTGTGGCAG TTTGCTACCA CCCTCTCTGC GATCACCTTT TTGCTTTAGA 
CAGTCCTCAG TGCCTGATGG CAGATCATCA GGTTCTAGAC TAACAGTTAA CATCCCAGTC 
AAGGCAAATT CATTAGATGG ATTTGCCCAA CATCACCAGG ATTCCTTAAG TGTACAGCCA 
GTCAGTACTG TGGCTTCTTC AGGTCTTTGC AAGTCTGACT CATGCCTGTA CCAAAGATGC 
AAGACTGACC AGATAACAGA TATGTTGATT CACGAGACGT GGGCAAGCTC TATTGAATCT 
CTAATGCGCA AAAACAAAAT CATAACAGAT GAGGCAGAGG TTGCAGAGGT AGATCATTTT 
CACAGTGATT CCCCACCACA TGTGCAACAG TATGCAAACA GACTGGCTGC AAATATTGTT 
GAAAGTGGTA AAAATTTAAT TGTTGTCCAG CAGGATGCCT TTGATTCTGC AAGCCAAGAA 
CACATGCTGG AAAGCAAACA TCCCCAGAGT GCAACTCAGA TTCAAGTAGA ACCCAAAAGG 
AGTGGAATAA ATTTGGATGA GCATATGAAG AACCCTGAAT GCCTCCCTGC AGGTCAGCAC 
AGGGAAGTGC CTTTAATTCA GATAGAAACT GATCAACGAG ACGAGTCAGA TAAAGACTCT 
GAATCCTTAA CTTTACATGA GCTTTCTGAA AAGGAGCATC AAGGCAAGGA AAAGCCTCCA 
GAAGCTTATG CTGGGAAGCA CATGGTTTCC AGTACCCTAC TGAATAGCAA AAGCAGTCAG 
AGCAAACCAG ATGCTGAAAT AACGGGAGAG ACAAAAGCAG CTGAAGAATT TCCAACACAC 
CTCAGCAGCA GTGAAGAAAG CACTGGCAGC TGGTCCCAGC TAGCCAATGA TGAAGACAAT 
CCTGATGACA CAAGTAGCTA CTTACAACTC AGCGAACGAT CTCTGAGCAA TGGCAACAGC 
AGTACAACTA GCAGTCTTGG CATTATGGAC CTGGAAATTT ATCAGGAGAA CGTGCCACCT 
TCTCCTATGA TTAATGACTT AGTAGAAGAA AAGGTTTTCC TTAAAGAACA GACAGATAAT 
ACAGAGGAAA GTACTTCTGG GCTCTCAGTG GGAACAGCCA ATTATCAGAA AGAACTCCTG 
GTGATTAATT TTGATCTGGA ACCAGAATGC CCCAACGCAG AGCTGCGAGC CACCCTACAG 
TGGATAGCTG CTTCTGAACT TGGAATCCCA ACCATCTATT TTAAAAAATC TCAGGAAAAC 
AGAATTGAAA AGTTTTTAGA TGTTGTGCAG CTAGTTCAAC GGAAGTCCTG GAAAGTGGGT 
GACATCTTTC ATGCTGTGGT GCAGTACTGC AAACTCAGTG AGGAGCGCAA AGAGAAGACA 
CCAAGCCTGT TCGATTGGCT CCTTGAACTG GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132375.1
CDS	264..5339
Translation	MFRPATRRHPALRSNFESPLMQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENVPPSPMINDLVEEKVFLKEQTDNTEESTSGLSVGTANYQKELLVINFDLEPECPNAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFHAVVQYCKLSEERKEKTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_015276889.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276889.2

ORF Insert Sequence:

ATGTTCCGCC CGGCCACCCG CCGCCACCCG GCGCTTCGCA GCAACTTTGA ATCTCCACTG 
ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGCCCA CGAAAAAATA 
AGTGTTTCAG AAGCATCTTC TGATGTCAGA AAAAATAAAG GAAAAGGATT TGAAAGTCTC 
CACTACAGAA AAGCCAGGTT GCCATTAATT CTTGAAGGGA ATTGTGTTAA TGAAGATAAT 
ACAGCTAGTA AAGTCTCTGA ATCTGTGCAC GTTGTGGCTG AAGATGGCAT CTCACAACCT 
GAAGATAAGC CAGAGCAGGA AATCCTGTGT CAAGAGGAAT TAGTGGGAAG AGATACATCA 
TCCCTCAGTA CTGAGGATTT GACTAACAAT GTTGAAAATT CCTGCTCAGT GAATGTGTTA 
GATGTGTCTT TAACCAAAAT GGCTAAAGAG GAGCTGGGGC CTCTTTATGG CTCTACAGCA 
AAACCCAACA GTAAGGCAGA GGTAGAGGAT TGTGCAGGTG TTAGGAAAAG CGACCCTCCC 
TTGCAAAACG AGCAAGCAGC TACAGGTCAG TACGCTACAA ATCTAGCAGA ATCTGTTCTG 
CAAGATGCAT TCATCAGACT ATCACAGTCT CAACCCACTT TCAGTGAGGA GGCTGCAGTC 
AGCATCTCCG TAGGAAGCTC CTGCAAGTCA AAAGATGCAT CTGCTTCCCG ATCGTGGAAT 
GAACTTCCAA AGATTGTCAT AGTGCAAAGT CCAGACAGCA GTGAGAATGT GCCAGACTGG 
CCAGGGTCTG CCTTCCCAAA CCTGTGCCAC TGGACTGAAA CAGAAAGTTC TGAAGTTTCA 
GATTACTTTG AAGAAGACCA TTCAAATGGA CGCAGCCAGA GTGCACTGGA GATGGCTCTG 
GCTTGTGCAG CAACTGTCAT TGGAACCATT TCAAGTCCAC AGGCAGCAGA AAAATTCAGA 
AGGGATCAAG AAGCCACAGA CTCTAAAAGC GAAACAGTTG ATAACGAAGA TCTACACGCA 
GATTCTTCAC AGCTACTTGA AGACTGTACT AGCACAGATT ATTCATTTCC ATCTGCACTG 
TGTGGCATGA CTCAAGTAGC AAGTGCTGTA GCAGTCTGCG GTCTGGGGGA AACAAAGGAA 
GAGAAGTACC CTACAACTTC AAGCGGGCTG CTGTCTGCAG CTCAGACTTC TGCAGCCATT 
ACTCTTCATT GTAGCATAGC TGTAGGAAGC AGCATGGAAA AGCTGAATGA TAGCATTGCA 
GAGGCCCTAC TCAAAGAGGC ATCAATAATT TTGACAAAAC CGAATACATA CAAAAATGTA 
GGGCATTTTA TGGAATCCAT AAACGGAAGA ATTATTGAAA CAGCAGCAAG GCCACGGATT 
CCACACTCCG ATGAAGTAAT CAGAGATGAA CTGGCACAAA ATTTATCCAA TATTATTCTA 
CGACATTCTA TTGAAGAGGT CAGGAAGAAA AGAGAGCTAC AGTCCTGTTC AGAAAACAGC 
TCAAGTACAC AAGACATTTT CATGGAGACT GCCAATGAGT TGCTTTTTAA TGTGATATAT 
TTCACTTGCA AGAAGATGAA TGACATAAGA CAAGTTGAAG AGTGTTCTCC TCTCTTTTCT 
GAAGGCTTGA AAGGCAAGAA GGTAACAAGA GTAGAAGGAT GGCCAACACA GGCAACAGCA 
TGTGAAACAT CACACAGACC CCTTGATCAT TCTGCTATTA AGCCATTTGG TACATCCTAC 
AGTGCTAGCA CAGATAGTGA AAATGGTATC AGCACGAAGA AAATTAACAT GAGAGATGTA 
AATAGCAATG AAAACGCCAC AGTGAATTCA GAGCTAACAA GTAGAGTCAT TGGGCATAAT 
GCACTTGTTG CAAAAACATC TCCCAAGAAA AGATACCTGA AAAGAACCAC ACGAGACTGC 
TACAAATCCC CACATCAGAA TAACATTCAG AAGAAGAGAG ATTACAGATC ATTTTCAGAC 
AGAGAAAGTA CCTTTGCAAA CAATGAATGC AGGCACGGTG TTCAAGAACA ACTGTCTTCC 
AGCGCTGTCG TGAATGCAGA AAACCAGGCC AAACATAAGT GTGATGCTGT GCTAAGTGAT 
GAAGTCCAAG TTAGCTTGTC TTTGTTAGGA AACCATGTCT TACTTCCTTC TCAGCCTGTG 
CTACAGGTGA AAAATTCAAG AGACAAATAC TGTATAACAG ATTTTGCAGA AGAATTGGCA 
GAAACAGTGG TCTCTATGGC AACAGAAATA GCTGCCATTT GTCTTGAAAA CTCAAATGGA 
AAGCAGCCCT GGTTCTGTGC ATGGAAGAGA GGCAATGAAT ATCTGGTGAC CCAGAGTATA 
TCATGCAGAA CTATGAAAAG GAAAAAGGAA ACCCATGCTA ATGGCTCAGT TGTTCGGAAA 
CACAGGGCAC CTCGACTGAG TGAGATCAAA AGAAAGACAG ATGAGCATCC TGAGCTAAAG 
GAAAGGTTGA TGAATCGAGT AGTAGATGAA TCTATAAACC TTGAGGACAC ACCAGATTCT 
GTCAATATAT TTGCAAATGA AGTGGCTGCA AAGATCATGA ACCTCACTGA ACTCTCCATG 
GTTGACAGTA TCTGGCAAGG TCCAAACCAT CCCAGGAACA GGCTGCACTG TGAAAGATGG 
AGCCGAGCCA AGGCCTCAAG CTGTGAGAGC ATACCAGAGG AGGACATGGA TTCCAAAGCC 
TCTGCCAATA CTCTAGGCCT AATGAATGCC CTTGGTCAGC CTGTGAGCCA GACAAGTTCC 
GTCTCAAAGC AGTCTAGTTG TGAAAGCATT ACAGATGAGT TTTCAAGATT TATGGTGAAC 
CAAATGGAAA ATGAGGGAAG AGGCTTTGAT TTATTACTAG ATTATTATGC AGGAAAAAAT 
GCAAACAACA TCTTAACGTC GGCTTTGCAA CAGGTTGCTA AGAAAAATGG TCATCTTAAT 
GTACGGCCAA GTTGCCCATC CAAGCAGTCC AGCACGGAAA GCATAACAGA AGAATTTTAC 
AGGTATATGC TAAAAGAAAT AGAAAAGGAA AATAAAGATA ATGCATCTTC CCCTCGGAAT 
TCAAAGGACT GGTGTGGCAG TTTGCTACCA CCCTCTCTGC GATCACCTTT TTGCTTTAGA 
CAGTCCTCAG TGCCTGATGG CAGATCATCA GGTTCTAGAC TAACAGTTAA CATCCCAGTC 
AAGGCAAATT CATTAGATGG ATTTGCCCAA CATCACCAGG ATTCCTTAAG TGTACAGCCA 
GTCAGTACTG TGGCTTCTTC AGGTCTTTGC AAGTCTGACT CATGCCTGTA CCAAAGATGC 
AAGACTGACC AGATAACAGA TATGTTGATT CACGAGACGT GGGCAAGCTC TATTGAATCT 
CTAATGCGCA AAAACAAAAT CATAACAGAT GAGGCAGAGG TTGCAGAGGT AGATCATTTT 
CACAGTGATT CCCCACCACA TGTGCAACAG TATGCAAACA GACTGGCTGC AAATATTGTT 
GAAAGTGGTA AAAATTTAAT TGTTGTCCAG CAGGATGCCT TTGATTCTGC AAGCCAAGAA 
CACATGCTGG AAAGCAAACA TCCCCAGAGT GCAACTCAGA TTCAAGTAGA ACCCAAAAGG 
AGTGGAATAA ATTTGGATGA GCATATGAAG AACCCTGAAT GCCTCCCTGC AGGTCAGCAC 
AGGGAAGTGC CTTTAATTCA GATAGAAACT GATCAACGAG ACGAGTCAGA TAAAGACTCT 
GAATCCTTAA CTTTACATGA GCTTTCTGAA AAGGAGCATC AAGGCAAGGA AAAGCCTCCA 
GAAGCTTATG CTGGGAAGCA CATGGTTTCC AGTACCCTAC TGAATAGCAA AAGCAGTCAG 
AGCAAACCAG ATGCTGAAAT AACGGGAGAG ACAAAAGCAG CTGAAGAATT TCCAACACAC 
CTCAGCAGCA GTGAAGAAAG CACTGGCAGC TGGTCCCAGC TAGCCAATGA TGAAGACAAT 
CCTGATGACA CAAGTAGCTA CTTACAACTC AGCGAACGAT CTCTGAGCAA TGGCAACAGC 
AGTACAACTA GCAGTCTTGG CATTATGGAC CTGGAAATTT ATCAGGAGAA CGTGCCACCT 
TCTCCTATGA TTAATGACTT AGTAGAAGAA AAGGTTTTCC TTAAAGAACA GACAGATAAT 
ACAGAGGAAA GTACTTCTGG GCTCTCAGTG GGAACAGCCA ATTATCAGAA AGAACTCCTG 
GTGATTAATT TTGATCTGGA ACCAGAATGC CCCAACGCAG AGCTGCGAGC CACCCTACAG 
TGGATAGCTG CTTCTGAACT TGGAATCCCA ACCATCTATT TTAAAAAATC TCAGGAAAAC 
AGAATTGAAA AGTTTTTAGA TGTTGTGCAG CTAGTTCAAC GGAAGTCCTG GAAAGTGGGT 
GACATCTTTC ATGCTGTGGT GCAGTACTGC AAACTCAGTG AGGAGCGCAA AGAGAAGACA 
CCAAGCCTGT TCGATTGGCT CCTTGAACTG GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_015276890.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132376.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.

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CloneID	OGa39664
Clone ID Related Accession (Same CDS sequence)	XM_015276890.1
Accession Version	XM_015276890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGCC GCCCCCCGCC CAGCAACTTT GAATCTCCAC TGATGCAAGA AGTCCCAGAG 
CACCAAGGCA ACAGCACAGA TAGTTCGGGA AGCAGTTTGG GAAGCACTGT CACAGCATGT 
AAAAAGATCC TCTGCAGTAA TAGCCTGCTA GAATCAACAG ACTACTGGCT GCAGAATCAG 
AGGACACCAT GCCAAATTGG CTTTCTGGAA GACAAGTCAG AAAGCAACTG TGCCTCAGTT 
TGTTTTGTGA ATTTAGATGC AAACAGAGAC GATTGCAGCA ATGAACAAGT GAAACAGAAA 
TTGATCAGCG TCTCTCCAAA TCTCCCTAAA CTCATCGGAT CTATGAATGT ACAACCACCA 
AAGGAAAATG AAATAGTCCT ACTGAGCGGA TTAACGTCAG GAAATCTTCA GGCTGATTAT 
GAAGTTCCCC AGTGTCCTTG GCAAGCAGAT GTCTGCTTGG TTCAGTGTGC GAGGGGAGAC 
AGGAAGAACA GTGCAAGCTG CATCATCTTT GAAATAAACA AGTTTCTGAT TGGGCTTGAG 
CTTGTTCAGG AGAGACAGCT GCAGATAGAA GCTCATGTCT CAAAGCCTGA GGACGACACA 
AACTGCTCAG TGTCCTCAAT AGAAGAAGAT TTCCTCACAG CATCTGAGCA TCTCGAGGAG 
GAAAACGAGG CTGATGAATA TAAAACTGTA GCCCACGAAA AAATAAGTGT TTCAGAAGCA 
TCTTCTGATG TCAGAAAAAA TAAAGGAAAA GGATTTGAAA GTCTCCACTA CAGAAAAGCC 
AGGTTGCCAT TAATTCTTGA AGGGAATTGT GTTAATGAAG ATAATACAGC TAGTAAAGTC 
TCTGAATCTG TGCACGTTGT GGCTGAAGAT GGCATCTCAC AACCTGAAGA TAAGCCAGAG 
CAGGAAATCC TGTGTCAAGA GGAATTAGTG GGAAGAGATA CATCATCCCT CAGTACTGAG 
GATTTGACTA ACAATGTTGA AAATTCCTGC TCAGTGAATG TGTTAGATGT GTCTTTAACC 
AAAATGGCTA AAGAGGAGCT GGGGCCTCTT TATGGCTCTA CAGCAAAACC CAACAGTAAG 
GCAGAGGTAG AGGATTGTGC AGGTGTTAGG AAAAGCGACC CTCCCTTGCA AAACGAGCAA 
GCAGCTACAG GTCAGTACGC TACAAATCTA GCAGAATCTG TTCTGCAAGA TGCATTCATC 
AGACTATCAC AGTCTCAACC CACTTTCAGT GAGGAGGCTG CAGTCAGCAT CTCCGTAGGA 
AGCTCCTGCA AGTCAAAAGA TGCATCTGCT TCCCGATCGT GGAATGAACT TCCAAAGATT 
GTCATAGTGC AAAGTCCAGA CAGCAGTGAG AATGTGCCAG ACTGGCCAGG GTCTGCCTTC 
CCAAACCTGT GCCACTGGAC TGAAACAGAA AGTTCTGAAG TTTCAGATTA CTTTGAAGAA 
GACCATTCAA ATGGACGCAG CCAGAGTGCA CTGGAGATGG CTCTGGCTTG TGCAGCAACT 
GTCATTGGAA CCATTTCAAG TCCACAGGCA GCAGAAAAAT TCAGAAGGGA TCAAGAAGCC 
ACAGACTCTA AAAGCGAAAC AGTTGATAAC GAAGATCTAC ACGCAGATTC TTCACAGCTA 
CTTGAAGACT GTACTAGCAC AGATTATTCA TTTCCATCTG CACTGTGTGG CATGACTCAA 
GTAGCAAGTG CTGTAGCAGT CTGCGGTCTG GGGGAAACAA AGGAAGAGAA GTACCCTACA 
ACTTCAAGCG GGCTGCTGTC TGCAGCTCAG ACTTCTGCAG CCATTACTCT TCATTGTAGC 
ATAGCTGTAG GAAGCAGCAT GGAAAAGCTG AATGATAGCA TTGCAGAGGC CCTACTCAAA 
GAGGCATCAA TAATTTTGAC AAAACCGAAT ACATACAAAA ATGTAGGGCA TTTTATGGAA 
TCCATAAACG GAAGAATTAT TGAAACAGCA GCAAGGCCAC GGATTCCACA CTCCGATGAA 
GTAATCAGAG ATGAACTGGC ACAAAATTTA TCCAATATTA TTCTACGACA TTCTATTGAA 
GAGGTCAGGA AGAAAAGAGA GCTACAGTCC TGTTCAGAAA ACAGCTCAAG TACACAAGAC 
ATTTTCATGG AGACTGCCAA TGAGTTGCTT TTTAATGTGA TATATTTCAC TTGCAAGAAG 
ATGAATGACA TAAGACAAGT TGAAGAGTGT TCTCCTCTCT TTTCTGAAGG CTTGAAAGGC 
AAGAAGGTAA CAAGAGTAGA AGGATGGCCA ACACAGGCAA CAGCATGTGA AACATCACAC 
AGACCCCTTG ATCATTCTGC TATTAAGCCA TTTGGTACAT CCTACAGTGC TAGCACAGAT 
AGTGAAAATG GTATCAGCAC GAAGAAAATT AACATGAGAG ATGTAAATAG CAATGAAAAC 
GCCACAGTGA ATTCAGAGCT AACAAGTAGA GTCATTGGGC ATAATGCACT TGTTGCAAAA 
ACATCTCCCA AGAAAAGATA CCTGAAAAGA ACCACACGAG ACTGCTACAA ATCCCCACAT 
CAGAATAACA TTCAGAAGAA GAGAGATTAC AGATCATTTT CAGACAGAGA AAGTACCTTT 
GCAAACAATG AATGCAGGCA CGGTGTTCAA GAACAACTGT CTTCCAGCGC TGTCGTGAAT 
GCAGAAAACC AGGCCAAACA TAAGTGTGAT GCTGTGCTAA GTGATGAAGT CCAAGTTAGC 
TTGTCTTTGT TAGGAAACCA TGTCTTACTT CCTTCTCAGC CTGTGCTACA GGTGAAAAAT 
TCAAGAGACA AATACTGTAT AACAGATTTT GCAGAAGAAT TGGCAGAAAC AGTGGTCTCT 
ATGGCAACAG AAATAGCTGC CATTTGTCTT GAAAACTCAA ATGGAAAGCA GCCCTGGTTC 
TGTGCATGGA AGAGAGGCAA TGAATATCTG GTGACCCAGA GTATATCATG CAGAACTATG 
AAAAGGAAAA AGGAAACCCA TGCTAATGGC TCAGTTGTTC GGAAACACAG GGCACCTCGA 
CTGAGTGAGA TCAAAAGAAA GACAGATGAG CATCCTGAGC TAAAGGAAAG GTTGATGAAT 
CGAGTAGTAG ATGAATCTAT AAACCTTGAG GACACACCAG ATTCTGTCAA TATATTTGCA 
AATGAAGTGG CTGCAAAGAT CATGAACCTC ACTGAACTCT CCATGGTTGA CAGTATCTGG 
CAAGGTCCAA ACCATCCCAG GAACAGGCTG CACTGTGAAA GATGGAGCCG AGCCAAGGCC 
TCAAGCTGTG AGAGCATACC AGAGGAGGAC ATGGATTCCA AAGCCTCTGC CAATACTCTA 
GGCCTAATGA ATGCCCTTGG TCAGCCTGTG AGCCAGACAA GTTCCGTCTC AAAGCAGTCT 
AGTTGTGAAA GCATTACAGA TGAGTTTTCA AGATTTATGG TGAACCAAAT GGAAAATGAG 
GGAAGAGGCT TTGATTTATT ACTAGATTAT TATGCAGGAA AAAATGCAAA CAACATCTTA 
ACGTCGGCTT TGCAACAGGT TGCTAAGAAA AATGGTCATC TTAATGTACG GCCAAGTTGC 
CCATCCAAGC AGTCCAGCAC GGAAAGCATA ACAGAAGAAT TTTACAGGTA TATGCTAAAA 
GAAATAGAAA AGGAAAATAA AGATAATGCA TCTTCCCCTC GGAATTCAAA GGACTGGTGT 
GGCAGTTTGC TACCACCCTC TCTGCGATCA CCTTTTTGCT TTAGACAGTC CTCAGTGCCT 
GATGGCAGAT CATCAGGTTC TAGACTAACA GTTAACATCC CAGTCAAGGC AAATTCATTA 
GATGGATTTG CCCAACATCA CCAGGATTCC TTAAGTGTAC AGCCAGTCAG TACTGTGGCT 
TCTTCAGGTC TTTGCAAGTC TGACTCATGC CTGTACCAAA GATGCAAGAC TGACCAGATA 
ACAGATATGT TGATTCACGA GACGTGGGCA AGCTCTATTG AATCTCTAAT GCGCAAAAAC 
AAAATCATAA CAGATGAGGC AGAGGTTGCA GAGGTAGATC ATTTTCACAG TGATTCCCCA 
CCACATGTGC AACAGTATGC AAACAGACTG GCTGCAAATA TTGTTGAAAG TGGTAAAAAT 
TTAATTGTTG TCCAGCAGGA TGCCTTTGAT TCTGCAAGCC AAGAACACAT GCTGGAAAGC 
AAACATCCCC AGAGTGCAAC TCAGATTCAA GTAGAACCCA AAAGGAGTGG AATAAATTTG 
GATGAGCATA TGAAGAACCC TGAATGCCTC CCTGCAGGTC AGCACAGGGA AGTGCCTTTA 
ATTCAGATAG AAACTGATCA ACGAGACGAG TCAGATAAAG ACTCTGAATC CTTAACTTTA 
CATGAGCTTT CTGAAAAGGA GCATCAAGGC AAGGAAAAGC CTCCAGAAGC TTATGCTGGG 
AAGCACATGG TTTCCAGTAC CCTACTGAAT AGCAAAAGCA GTCAGAGCAA ACCAGATGCT 
GAAATAACGG GAGAGACAAA AGCAGCTGAA GAATTTCCAA CACACCTCAG CAGCAGTGAA 
GAAAGCACTG GCAGCTGGTC CCAGCTAGCC AATGATGAAG ACAATCCTGA TGACACAAGT 
AGCTACTTAC AACTCAGCGA ACGATCTCTG AGCAATGGCA ACAGCAGTAC AACTAGCAGT 
CTTGGCATTA TGGACCTGGA AATTTATCAG GAGAACGTGC CACCTTCTCC TATGATTAAT 
GACTTAGTAG AAGAAAAGGT TTTCCTTAAA GAACAGACAG ATAATACAGA GGAAAGTACT 
TCTGGGCTCT CAGTGGGAAC AGCCAATTAT CAGAAAGAAC TCCTGGTGAT TAATTTTGAT 
CTGGAACCAG AATGCCCCAA CGCAGAGCTG CGAGCCACCC TACAGTGGAT AGCTGCTTCT 
GAACTTGGAA TCCCAACCAT CTATTTTAAA AAATCTCAGG AAAACAGAAT TGAAAAGTTT 
TTAGATGTTG TGCAGCTAGT TCAACGGAAG TCCTGGAAAG TGGGTGACAT CTTTCATGCT 
GTGGTGCAGT ACTGCAAACT CAGTGAGGAG CGCAAAGAGA AGACACCAAG CCTGTTCGAT 
TGGCTCCTTG AACTGGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132376.1
CDS	124..5184
Translation	MAGRPPPSNFESPLMQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTVAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENVPPSPMINDLVEEKVFLKEQTDNTEESTSGLSVGTANYQKELLVINFDLEPECPNAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFHAVVQYCKLSEERKEKTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_015276890.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276890.1

ORF Insert Sequence:

ATGGCCGGCC GCCCCCCGCC CAGCAACTTT GAATCTCCAC TGATGCAAGA AGTCCCAGAG 
CACCAAGGCA ACAGCACAGA TAGTTCGGGA AGCAGTTTGG GAAGCACTGT CACAGCATGT 
AAAAAGATCC TCTGCAGTAA TAGCCTGCTA GAATCAACAG ACTACTGGCT GCAGAATCAG 
AGGACACCAT GCCAAATTGG CTTTCTGGAA GACAAGTCAG AAAGCAACTG TGCCTCAGTT 
TGTTTTGTGA ATTTAGATGC AAACAGAGAC GATTGCAGCA ATGAACAAGT GAAACAGAAA 
TTGATCAGCG TCTCTCCAAA TCTCCCTAAA CTCATCGGAT CTATGAATGT ACAACCACCA 
AAGGAAAATG AAATAGTCCT ACTGAGCGGA TTAACGTCAG GAAATCTTCA GGCTGATTAT 
GAAGTTCCCC AGTGTCCTTG GCAAGCAGAT GTCTGCTTGG TTCAGTGTGC GAGGGGAGAC 
AGGAAGAACA GTGCAAGCTG CATCATCTTT GAAATAAACA AGTTTCTGAT TGGGCTTGAG 
CTTGTTCAGG AGAGACAGCT GCAGATAGAA GCTCATGTCT CAAAGCCTGA GGACGACACA 
AACTGCTCAG TGTCCTCAAT AGAAGAAGAT TTCCTCACAG CATCTGAGCA TCTCGAGGAG 
GAAAACGAGG CTGATGAATA TAAAACTGTA GCCCACGAAA AAATAAGTGT TTCAGAAGCA 
TCTTCTGATG TCAGAAAAAA TAAAGGAAAA GGATTTGAAA GTCTCCACTA CAGAAAAGCC 
AGGTTGCCAT TAATTCTTGA AGGGAATTGT GTTAATGAAG ATAATACAGC TAGTAAAGTC 
TCTGAATCTG TGCACGTTGT GGCTGAAGAT GGCATCTCAC AACCTGAAGA TAAGCCAGAG 
CAGGAAATCC TGTGTCAAGA GGAATTAGTG GGAAGAGATA CATCATCCCT CAGTACTGAG 
GATTTGACTA ACAATGTTGA AAATTCCTGC TCAGTGAATG TGTTAGATGT GTCTTTAACC 
AAAATGGCTA AAGAGGAGCT GGGGCCTCTT TATGGCTCTA CAGCAAAACC CAACAGTAAG 
GCAGAGGTAG AGGATTGTGC AGGTGTTAGG AAAAGCGACC CTCCCTTGCA AAACGAGCAA 
GCAGCTACAG GTCAGTACGC TACAAATCTA GCAGAATCTG TTCTGCAAGA TGCATTCATC 
AGACTATCAC AGTCTCAACC CACTTTCAGT GAGGAGGCTG CAGTCAGCAT CTCCGTAGGA 
AGCTCCTGCA AGTCAAAAGA TGCATCTGCT TCCCGATCGT GGAATGAACT TCCAAAGATT 
GTCATAGTGC AAAGTCCAGA CAGCAGTGAG AATGTGCCAG ACTGGCCAGG GTCTGCCTTC 
CCAAACCTGT GCCACTGGAC TGAAACAGAA AGTTCTGAAG TTTCAGATTA CTTTGAAGAA 
GACCATTCAA ATGGACGCAG CCAGAGTGCA CTGGAGATGG CTCTGGCTTG TGCAGCAACT 
GTCATTGGAA CCATTTCAAG TCCACAGGCA GCAGAAAAAT TCAGAAGGGA TCAAGAAGCC 
ACAGACTCTA AAAGCGAAAC AGTTGATAAC GAAGATCTAC ACGCAGATTC TTCACAGCTA 
CTTGAAGACT GTACTAGCAC AGATTATTCA TTTCCATCTG CACTGTGTGG CATGACTCAA 
GTAGCAAGTG CTGTAGCAGT CTGCGGTCTG GGGGAAACAA AGGAAGAGAA GTACCCTACA 
ACTTCAAGCG GGCTGCTGTC TGCAGCTCAG ACTTCTGCAG CCATTACTCT TCATTGTAGC 
ATAGCTGTAG GAAGCAGCAT GGAAAAGCTG AATGATAGCA TTGCAGAGGC CCTACTCAAA 
GAGGCATCAA TAATTTTGAC AAAACCGAAT ACATACAAAA ATGTAGGGCA TTTTATGGAA 
TCCATAAACG GAAGAATTAT TGAAACAGCA GCAAGGCCAC GGATTCCACA CTCCGATGAA 
GTAATCAGAG ATGAACTGGC ACAAAATTTA TCCAATATTA TTCTACGACA TTCTATTGAA 
GAGGTCAGGA AGAAAAGAGA GCTACAGTCC TGTTCAGAAA ACAGCTCAAG TACACAAGAC 
ATTTTCATGG AGACTGCCAA TGAGTTGCTT TTTAATGTGA TATATTTCAC TTGCAAGAAG 
ATGAATGACA TAAGACAAGT TGAAGAGTGT TCTCCTCTCT TTTCTGAAGG CTTGAAAGGC 
AAGAAGGTAA CAAGAGTAGA AGGATGGCCA ACACAGGCAA CAGCATGTGA AACATCACAC 
AGACCCCTTG ATCATTCTGC TATTAAGCCA TTTGGTACAT CCTACAGTGC TAGCACAGAT 
AGTGAAAATG GTATCAGCAC GAAGAAAATT AACATGAGAG ATGTAAATAG CAATGAAAAC 
GCCACAGTGA ATTCAGAGCT AACAAGTAGA GTCATTGGGC ATAATGCACT TGTTGCAAAA 
ACATCTCCCA AGAAAAGATA CCTGAAAAGA ACCACACGAG ACTGCTACAA ATCCCCACAT 
CAGAATAACA TTCAGAAGAA GAGAGATTAC AGATCATTTT CAGACAGAGA AAGTACCTTT 
GCAAACAATG AATGCAGGCA CGGTGTTCAA GAACAACTGT CTTCCAGCGC TGTCGTGAAT 
GCAGAAAACC AGGCCAAACA TAAGTGTGAT GCTGTGCTAA GTGATGAAGT CCAAGTTAGC 
TTGTCTTTGT TAGGAAACCA TGTCTTACTT CCTTCTCAGC CTGTGCTACA GGTGAAAAAT 
TCAAGAGACA AATACTGTAT AACAGATTTT GCAGAAGAAT TGGCAGAAAC AGTGGTCTCT 
ATGGCAACAG AAATAGCTGC CATTTGTCTT GAAAACTCAA ATGGAAAGCA GCCCTGGTTC 
TGTGCATGGA AGAGAGGCAA TGAATATCTG GTGACCCAGA GTATATCATG CAGAACTATG 
AAAAGGAAAA AGGAAACCCA TGCTAATGGC TCAGTTGTTC GGAAACACAG GGCACCTCGA 
CTGAGTGAGA TCAAAAGAAA GACAGATGAG CATCCTGAGC TAAAGGAAAG GTTGATGAAT 
CGAGTAGTAG ATGAATCTAT AAACCTTGAG GACACACCAG ATTCTGTCAA TATATTTGCA 
AATGAAGTGG CTGCAAAGAT CATGAACCTC ACTGAACTCT CCATGGTTGA CAGTATCTGG 
CAAGGTCCAA ACCATCCCAG GAACAGGCTG CACTGTGAAA GATGGAGCCG AGCCAAGGCC 
TCAAGCTGTG AGAGCATACC AGAGGAGGAC ATGGATTCCA AAGCCTCTGC CAATACTCTA 
GGCCTAATGA ATGCCCTTGG TCAGCCTGTG AGCCAGACAA GTTCCGTCTC AAAGCAGTCT 
AGTTGTGAAA GCATTACAGA TGAGTTTTCA AGATTTATGG TGAACCAAAT GGAAAATGAG 
GGAAGAGGCT TTGATTTATT ACTAGATTAT TATGCAGGAA AAAATGCAAA CAACATCTTA 
ACGTCGGCTT TGCAACAGGT TGCTAAGAAA AATGGTCATC TTAATGTACG GCCAAGTTGC 
CCATCCAAGC AGTCCAGCAC GGAAAGCATA ACAGAAGAAT TTTACAGGTA TATGCTAAAA 
GAAATAGAAA AGGAAAATAA AGATAATGCA TCTTCCCCTC GGAATTCAAA GGACTGGTGT 
GGCAGTTTGC TACCACCCTC TCTGCGATCA CCTTTTTGCT TTAGACAGTC CTCAGTGCCT 
GATGGCAGAT CATCAGGTTC TAGACTAACA GTTAACATCC CAGTCAAGGC AAATTCATTA 
GATGGATTTG CCCAACATCA CCAGGATTCC TTAAGTGTAC AGCCAGTCAG TACTGTGGCT 
TCTTCAGGTC TTTGCAAGTC TGACTCATGC CTGTACCAAA GATGCAAGAC TGACCAGATA 
ACAGATATGT TGATTCACGA GACGTGGGCA AGCTCTATTG AATCTCTAAT GCGCAAAAAC 
AAAATCATAA CAGATGAGGC AGAGGTTGCA GAGGTAGATC ATTTTCACAG TGATTCCCCA 
CCACATGTGC AACAGTATGC AAACAGACTG GCTGCAAATA TTGTTGAAAG TGGTAAAAAT 
TTAATTGTTG TCCAGCAGGA TGCCTTTGAT TCTGCAAGCC AAGAACACAT GCTGGAAAGC 
AAACATCCCC AGAGTGCAAC TCAGATTCAA GTAGAACCCA AAAGGAGTGG AATAAATTTG 
GATGAGCATA TGAAGAACCC TGAATGCCTC CCTGCAGGTC AGCACAGGGA AGTGCCTTTA 
ATTCAGATAG AAACTGATCA ACGAGACGAG TCAGATAAAG ACTCTGAATC CTTAACTTTA 
CATGAGCTTT CTGAAAAGGA GCATCAAGGC AAGGAAAAGC CTCCAGAAGC TTATGCTGGG 
AAGCACATGG TTTCCAGTAC CCTACTGAAT AGCAAAAGCA GTCAGAGCAA ACCAGATGCT 
GAAATAACGG GAGAGACAAA AGCAGCTGAA GAATTTCCAA CACACCTCAG CAGCAGTGAA 
GAAAGCACTG GCAGCTGGTC CCAGCTAGCC AATGATGAAG ACAATCCTGA TGACACAAGT 
AGCTACTTAC AACTCAGCGA ACGATCTCTG AGCAATGGCA ACAGCAGTAC AACTAGCAGT 
CTTGGCATTA TGGACCTGGA AATTTATCAG GAGAACGTGC CACCTTCTCC TATGATTAAT 
GACTTAGTAG AAGAAAAGGT TTTCCTTAAA GAACAGACAG ATAATACAGA GGAAAGTACT 
TCTGGGCTCT CAGTGGGAAC AGCCAATTAT CAGAAAGAAC TCCTGGTGAT TAATTTTGAT 
CTGGAACCAG AATGCCCCAA CGCAGAGCTG CGAGCCACCC TACAGTGGAT AGCTGCTTCT 
GAACTTGGAA TCCCAACCAT CTATTTTAAA AAATCTCAGG AAAACAGAAT TGAAAAGTTT 
TTAGATGTTG TGCAGCTAGT TCAACGGAAG TCCTGGAAAG TGGGTGACAT CTTTCATGCT 
GTGGTGCAGT ACTGCAAACT CAGTGAGGAG CGCAAAGAGA AGACACCAAG CCTGTTCGAT 
TGGCTCCTTG AACTGGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_015276891.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132377.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X4, mRNA.

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CloneID	OGa39665
Clone ID Related Accession (Same CDS sequence)	XM_015276891.1
Accession Version	XM_015276891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGCC GCCCCCCGCC CAGCAACTTT GAATCTCCAC TGATGCAAGA AGTCCCAGAG 
CACCAAGGCA ACAGCACAGA TAGTTCGGGA AGCAGTTTGG GAAGCACTGT CACAGCATGT 
AAAAAGATCC TCTGCAGTAA TAGCCTGCTA GAATCAACAG ACTACTGGCT GCAGAATCAG 
AGGACACCAT GCCAAATTGG CTTTCTGGAA GACAAGTCAG AAAGCAACTG TGCCTCAGTT 
TGTTTTGTGA ATTTAGATGC AAACAGAGAC GATTGCAGCA ATGAACAAGT GAAACAGAAA 
TTGATCAGCG TCTCTCCAAA TCTCCCTAAA CTCATCGGAT CTATGAATGT ACAACCACCA 
AAGGAAAATG AAATAGTCCT ACTGAGCGGA TTAACGTCAG GAAATCTTCA GGCTGATTAT 
GAAGTTCCCC AGTGTCCTTG GCAAGCAGAT GTCTGCTTGG TTCAGTGTGC GAGGGGAGAC 
AGGAAGAACA GTGCAAGCTG CATCATCTTT GAAATAAACA AGTTTCTGAT TGGGCTTGAG 
CTTGTTCAGG AGAGACAGCT GCAGATAGAA GCTCATGTCT CAAAGCCTGA GGACGACACA 
AACTGCTCAG TGTCCTCAAT AGAAGAAGAT TTCCTCACAG CATCTGAGCA TCTCGAGGAG 
GAAAACGAGG CTGATGAATA TAAAACTGCC CACGAAAAAA TAAGTGTTTC AGAAGCATCT 
TCTGATGTCA GAAAAAATAA AGGAAAAGGA TTTGAAAGTC TCCACTACAG AAAAGCCAGG 
TTGCCATTAA TTCTTGAAGG GAATTGTGTT AATGAAGATA ATACAGCTAG TAAAGTCTCT 
GAATCTGTGC ACGTTGTGGC TGAAGATGGC ATCTCACAAC CTGAAGATAA GCCAGAGCAG 
GAAATCCTGT GTCAAGAGGA ATTAGTGGGA AGAGATACAT CATCCCTCAG TACTGAGGAT 
TTGACTAACA ATGTTGAAAA TTCCTGCTCA GTGAATGTGT TAGATGTGTC TTTAACCAAA 
ATGGCTAAAG AGGAGCTGGG GCCTCTTTAT GGCTCTACAG CAAAACCCAA CAGTAAGGCA 
GAGGTAGAGG ATTGTGCAGG TGTTAGGAAA AGCGACCCTC CCTTGCAAAA CGAGCAAGCA 
GCTACAGGTC AGTACGCTAC AAATCTAGCA GAATCTGTTC TGCAAGATGC ATTCATCAGA 
CTATCACAGT CTCAACCCAC TTTCAGTGAG GAGGCTGCAG TCAGCATCTC CGTAGGAAGC 
TCCTGCAAGT CAAAAGATGC ATCTGCTTCC CGATCGTGGA ATGAACTTCC AAAGATTGTC 
ATAGTGCAAA GTCCAGACAG CAGTGAGAAT GTGCCAGACT GGCCAGGGTC TGCCTTCCCA 
AACCTGTGCC ACTGGACTGA AACAGAAAGT TCTGAAGTTT CAGATTACTT TGAAGAAGAC 
CATTCAAATG GACGCAGCCA GAGTGCACTG GAGATGGCTC TGGCTTGTGC AGCAACTGTC 
ATTGGAACCA TTTCAAGTCC ACAGGCAGCA GAAAAATTCA GAAGGGATCA AGAAGCCACA 
GACTCTAAAA GCGAAACAGT TGATAACGAA GATCTACACG CAGATTCTTC ACAGCTACTT 
GAAGACTGTA CTAGCACAGA TTATTCATTT CCATCTGCAC TGTGTGGCAT GACTCAAGTA 
GCAAGTGCTG TAGCAGTCTG CGGTCTGGGG GAAACAAAGG AAGAGAAGTA CCCTACAACT 
TCAAGCGGGC TGCTGTCTGC AGCTCAGACT TCTGCAGCCA TTACTCTTCA TTGTAGCATA 
GCTGTAGGAA GCAGCATGGA AAAGCTGAAT GATAGCATTG CAGAGGCCCT ACTCAAAGAG 
GCATCAATAA TTTTGACAAA ACCGAATACA TACAAAAATG TAGGGCATTT TATGGAATCC 
ATAAACGGAA GAATTATTGA AACAGCAGCA AGGCCACGGA TTCCACACTC CGATGAAGTA 
ATCAGAGATG AACTGGCACA AAATTTATCC AATATTATTC TACGACATTC TATTGAAGAG 
GTCAGGAAGA AAAGAGAGCT ACAGTCCTGT TCAGAAAACA GCTCAAGTAC ACAAGACATT 
TTCATGGAGA CTGCCAATGA GTTGCTTTTT AATGTGATAT ATTTCACTTG CAAGAAGATG 
AATGACATAA GACAAGTTGA AGAGTGTTCT CCTCTCTTTT CTGAAGGCTT GAAAGGCAAG 
AAGGTAACAA GAGTAGAAGG ATGGCCAACA CAGGCAACAG CATGTGAAAC ATCACACAGA 
CCCCTTGATC ATTCTGCTAT TAAGCCATTT GGTACATCCT ACAGTGCTAG CACAGATAGT 
GAAAATGGTA TCAGCACGAA GAAAATTAAC ATGAGAGATG TAAATAGCAA TGAAAACGCC 
ACAGTGAATT CAGAGCTAAC AAGTAGAGTC ATTGGGCATA ATGCACTTGT TGCAAAAACA 
TCTCCCAAGA AAAGATACCT GAAAAGAACC ACACGAGACT GCTACAAATC CCCACATCAG 
AATAACATTC AGAAGAAGAG AGATTACAGA TCATTTTCAG ACAGAGAAAG TACCTTTGCA 
AACAATGAAT GCAGGCACGG TGTTCAAGAA CAACTGTCTT CCAGCGCTGT CGTGAATGCA 
GAAAACCAGG CCAAACATAA GTGTGATGCT GTGCTAAGTG ATGAAGTCCA AGTTAGCTTG 
TCTTTGTTAG GAAACCATGT CTTACTTCCT TCTCAGCCTG TGCTACAGGT GAAAAATTCA 
AGAGACAAAT ACTGTATAAC AGATTTTGCA GAAGAATTGG CAGAAACAGT GGTCTCTATG 
GCAACAGAAA TAGCTGCCAT TTGTCTTGAA AACTCAAATG GAAAGCAGCC CTGGTTCTGT 
GCATGGAAGA GAGGCAATGA ATATCTGGTG ACCCAGAGTA TATCATGCAG AACTATGAAA 
AGGAAAAAGG AAACCCATGC TAATGGCTCA GTTGTTCGGA AACACAGGGC ACCTCGACTG 
AGTGAGATCA AAAGAAAGAC AGATGAGCAT CCTGAGCTAA AGGAAAGGTT GATGAATCGA 
GTAGTAGATG AATCTATAAA CCTTGAGGAC ACACCAGATT CTGTCAATAT ATTTGCAAAT 
GAAGTGGCTG CAAAGATCAT GAACCTCACT GAACTCTCCA TGGTTGACAG TATCTGGCAA 
GGTCCAAACC ATCCCAGGAA CAGGCTGCAC TGTGAAAGAT GGAGCCGAGC CAAGGCCTCA 
AGCTGTGAGA GCATACCAGA GGAGGACATG GATTCCAAAG CCTCTGCCAA TACTCTAGGC 
CTAATGAATG CCCTTGGTCA GCCTGTGAGC CAGACAAGTT CCGTCTCAAA GCAGTCTAGT 
TGTGAAAGCA TTACAGATGA GTTTTCAAGA TTTATGGTGA ACCAAATGGA AAATGAGGGA 
AGAGGCTTTG ATTTATTACT AGATTATTAT GCAGGAAAAA ATGCAAACAA CATCTTAACG 
TCGGCTTTGC AACAGGTTGC TAAGAAAAAT GGTCATCTTA ATGTACGGCC AAGTTGCCCA 
TCCAAGCAGT CCAGCACGGA AAGCATAACA GAAGAATTTT ACAGGTATAT GCTAAAAGAA 
ATAGAAAAGG AAAATAAAGA TAATGCATCT TCCCCTCGGA ATTCAAAGGA CTGGTGTGGC 
AGTTTGCTAC CACCCTCTCT GCGATCACCT TTTTGCTTTA GACAGTCCTC AGTGCCTGAT 
GGCAGATCAT CAGGTTCTAG ACTAACAGTT AACATCCCAG TCAAGGCAAA TTCATTAGAT 
GGATTTGCCC AACATCACCA GGATTCCTTA AGTGTACAGC CAGTCAGTAC TGTGGCTTCT 
TCAGGTCTTT GCAAGTCTGA CTCATGCCTG TACCAAAGAT GCAAGACTGA CCAGATAACA 
GATATGTTGA TTCACGAGAC GTGGGCAAGC TCTATTGAAT CTCTAATGCG CAAAAACAAA 
ATCATAACAG ATGAGGCAGA GGTTGCAGAG GTAGATCATT TTCACAGTGA TTCCCCACCA 
CATGTGCAAC AGTATGCAAA CAGACTGGCT GCAAATATTG TTGAAAGTGG TAAAAATTTA 
ATTGTTGTCC AGCAGGATGC CTTTGATTCT GCAAGCCAAG AACACATGCT GGAAAGCAAA 
CATCCCCAGA GTGCAACTCA GATTCAAGTA GAACCCAAAA GGAGTGGAAT AAATTTGGAT 
GAGCATATGA AGAACCCTGA ATGCCTCCCT GCAGGTCAGC ACAGGGAAGT GCCTTTAATT 
CAGATAGAAA CTGATCAACG AGACGAGTCA GATAAAGACT CTGAATCCTT AACTTTACAT 
GAGCTTTCTG AAAAGGAGCA TCAAGGCAAG GAAAAGCCTC CAGAAGCTTA TGCTGGGAAG 
CACATGGTTT CCAGTACCCT ACTGAATAGC AAAAGCAGTC AGAGCAAACC AGATGCTGAA 
ATAACGGGAG AGACAAAAGC AGCTGAAGAA TTTCCAACAC ACCTCAGCAG CAGTGAAGAA 
AGCACTGGCA GCTGGTCCCA GCTAGCCAAT GATGAAGACA ATCCTGATGA CACAAGTAGC 
TACTTACAAC TCAGCGAACG ATCTCTGAGC AATGGCAACA GCAGTACAAC TAGCAGTCTT 
GGCATTATGG ACCTGGAAAT TTATCAGGAG AACGTGCCAC CTTCTCCTAT GATTAATGAC 
TTAGTAGAAG AAAAGGTTTT CCTTAAAGAA CAGACAGATA ATACAGAGGA AAGTACTTCT 
GGGCTCTCAG TGGGAACAGC CAATTATCAG AAAGAACTCC TGGTGATTAA TTTTGATCTG 
GAACCAGAAT GCCCCAACGC AGAGCTGCGA GCCACCCTAC AGTGGATAGC TGCTTCTGAA 
CTTGGAATCC CAACCATCTA TTTTAAAAAA TCTCAGGAAA ACAGAATTGA AAAGTTTTTA 
GATGTTGTGC AGCTAGTTCA ACGGAAGTCC TGGAAAGTGG GTGACATCTT TCATGCTGTG 
GTGCAGTACT GCAAACTCAG TGAGGAGCGC AAAGAGAAGA CACCAAGCCT GTTCGATTGG 
CTCCTTGAAC TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132377.1
CDS	124..5181
Translation	MAGRPPPSNFESPLMQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENVPPSPMINDLVEEKVFLKEQTDNTEESTSGLSVGTANYQKELLVINFDLEPECPNAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFHAVVQYCKLSEERKEKTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_015276891.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276891.1

ORF Insert Sequence:

ATGGCCGGCC GCCCCCCGCC CAGCAACTTT GAATCTCCAC TGATGCAAGA AGTCCCAGAG 
CACCAAGGCA ACAGCACAGA TAGTTCGGGA AGCAGTTTGG GAAGCACTGT CACAGCATGT 
AAAAAGATCC TCTGCAGTAA TAGCCTGCTA GAATCAACAG ACTACTGGCT GCAGAATCAG 
AGGACACCAT GCCAAATTGG CTTTCTGGAA GACAAGTCAG AAAGCAACTG TGCCTCAGTT 
TGTTTTGTGA ATTTAGATGC AAACAGAGAC GATTGCAGCA ATGAACAAGT GAAACAGAAA 
TTGATCAGCG TCTCTCCAAA TCTCCCTAAA CTCATCGGAT CTATGAATGT ACAACCACCA 
AAGGAAAATG AAATAGTCCT ACTGAGCGGA TTAACGTCAG GAAATCTTCA GGCTGATTAT 
GAAGTTCCCC AGTGTCCTTG GCAAGCAGAT GTCTGCTTGG TTCAGTGTGC GAGGGGAGAC 
AGGAAGAACA GTGCAAGCTG CATCATCTTT GAAATAAACA AGTTTCTGAT TGGGCTTGAG 
CTTGTTCAGG AGAGACAGCT GCAGATAGAA GCTCATGTCT CAAAGCCTGA GGACGACACA 
AACTGCTCAG TGTCCTCAAT AGAAGAAGAT TTCCTCACAG CATCTGAGCA TCTCGAGGAG 
GAAAACGAGG CTGATGAATA TAAAACTGCC CACGAAAAAA TAAGTGTTTC AGAAGCATCT 
TCTGATGTCA GAAAAAATAA AGGAAAAGGA TTTGAAAGTC TCCACTACAG AAAAGCCAGG 
TTGCCATTAA TTCTTGAAGG GAATTGTGTT AATGAAGATA ATACAGCTAG TAAAGTCTCT 
GAATCTGTGC ACGTTGTGGC TGAAGATGGC ATCTCACAAC CTGAAGATAA GCCAGAGCAG 
GAAATCCTGT GTCAAGAGGA ATTAGTGGGA AGAGATACAT CATCCCTCAG TACTGAGGAT 
TTGACTAACA ATGTTGAAAA TTCCTGCTCA GTGAATGTGT TAGATGTGTC TTTAACCAAA 
ATGGCTAAAG AGGAGCTGGG GCCTCTTTAT GGCTCTACAG CAAAACCCAA CAGTAAGGCA 
GAGGTAGAGG ATTGTGCAGG TGTTAGGAAA AGCGACCCTC CCTTGCAAAA CGAGCAAGCA 
GCTACAGGTC AGTACGCTAC AAATCTAGCA GAATCTGTTC TGCAAGATGC ATTCATCAGA 
CTATCACAGT CTCAACCCAC TTTCAGTGAG GAGGCTGCAG TCAGCATCTC CGTAGGAAGC 
TCCTGCAAGT CAAAAGATGC ATCTGCTTCC CGATCGTGGA ATGAACTTCC AAAGATTGTC 
ATAGTGCAAA GTCCAGACAG CAGTGAGAAT GTGCCAGACT GGCCAGGGTC TGCCTTCCCA 
AACCTGTGCC ACTGGACTGA AACAGAAAGT TCTGAAGTTT CAGATTACTT TGAAGAAGAC 
CATTCAAATG GACGCAGCCA GAGTGCACTG GAGATGGCTC TGGCTTGTGC AGCAACTGTC 
ATTGGAACCA TTTCAAGTCC ACAGGCAGCA GAAAAATTCA GAAGGGATCA AGAAGCCACA 
GACTCTAAAA GCGAAACAGT TGATAACGAA GATCTACACG CAGATTCTTC ACAGCTACTT 
GAAGACTGTA CTAGCACAGA TTATTCATTT CCATCTGCAC TGTGTGGCAT GACTCAAGTA 
GCAAGTGCTG TAGCAGTCTG CGGTCTGGGG GAAACAAAGG AAGAGAAGTA CCCTACAACT 
TCAAGCGGGC TGCTGTCTGC AGCTCAGACT TCTGCAGCCA TTACTCTTCA TTGTAGCATA 
GCTGTAGGAA GCAGCATGGA AAAGCTGAAT GATAGCATTG CAGAGGCCCT ACTCAAAGAG 
GCATCAATAA TTTTGACAAA ACCGAATACA TACAAAAATG TAGGGCATTT TATGGAATCC 
ATAAACGGAA GAATTATTGA AACAGCAGCA AGGCCACGGA TTCCACACTC CGATGAAGTA 
ATCAGAGATG AACTGGCACA AAATTTATCC AATATTATTC TACGACATTC TATTGAAGAG 
GTCAGGAAGA AAAGAGAGCT ACAGTCCTGT TCAGAAAACA GCTCAAGTAC ACAAGACATT 
TTCATGGAGA CTGCCAATGA GTTGCTTTTT AATGTGATAT ATTTCACTTG CAAGAAGATG 
AATGACATAA GACAAGTTGA AGAGTGTTCT CCTCTCTTTT CTGAAGGCTT GAAAGGCAAG 
AAGGTAACAA GAGTAGAAGG ATGGCCAACA CAGGCAACAG CATGTGAAAC ATCACACAGA 
CCCCTTGATC ATTCTGCTAT TAAGCCATTT GGTACATCCT ACAGTGCTAG CACAGATAGT 
GAAAATGGTA TCAGCACGAA GAAAATTAAC ATGAGAGATG TAAATAGCAA TGAAAACGCC 
ACAGTGAATT CAGAGCTAAC AAGTAGAGTC ATTGGGCATA ATGCACTTGT TGCAAAAACA 
TCTCCCAAGA AAAGATACCT GAAAAGAACC ACACGAGACT GCTACAAATC CCCACATCAG 
AATAACATTC AGAAGAAGAG AGATTACAGA TCATTTTCAG ACAGAGAAAG TACCTTTGCA 
AACAATGAAT GCAGGCACGG TGTTCAAGAA CAACTGTCTT CCAGCGCTGT CGTGAATGCA 
GAAAACCAGG CCAAACATAA GTGTGATGCT GTGCTAAGTG ATGAAGTCCA AGTTAGCTTG 
TCTTTGTTAG GAAACCATGT CTTACTTCCT TCTCAGCCTG TGCTACAGGT GAAAAATTCA 
AGAGACAAAT ACTGTATAAC AGATTTTGCA GAAGAATTGG CAGAAACAGT GGTCTCTATG 
GCAACAGAAA TAGCTGCCAT TTGTCTTGAA AACTCAAATG GAAAGCAGCC CTGGTTCTGT 
GCATGGAAGA GAGGCAATGA ATATCTGGTG ACCCAGAGTA TATCATGCAG AACTATGAAA 
AGGAAAAAGG AAACCCATGC TAATGGCTCA GTTGTTCGGA AACACAGGGC ACCTCGACTG 
AGTGAGATCA AAAGAAAGAC AGATGAGCAT CCTGAGCTAA AGGAAAGGTT GATGAATCGA 
GTAGTAGATG AATCTATAAA CCTTGAGGAC ACACCAGATT CTGTCAATAT ATTTGCAAAT 
GAAGTGGCTG CAAAGATCAT GAACCTCACT GAACTCTCCA TGGTTGACAG TATCTGGCAA 
GGTCCAAACC ATCCCAGGAA CAGGCTGCAC TGTGAAAGAT GGAGCCGAGC CAAGGCCTCA 
AGCTGTGAGA GCATACCAGA GGAGGACATG GATTCCAAAG CCTCTGCCAA TACTCTAGGC 
CTAATGAATG CCCTTGGTCA GCCTGTGAGC CAGACAAGTT CCGTCTCAAA GCAGTCTAGT 
TGTGAAAGCA TTACAGATGA GTTTTCAAGA TTTATGGTGA ACCAAATGGA AAATGAGGGA 
AGAGGCTTTG ATTTATTACT AGATTATTAT GCAGGAAAAA ATGCAAACAA CATCTTAACG 
TCGGCTTTGC AACAGGTTGC TAAGAAAAAT GGTCATCTTA ATGTACGGCC AAGTTGCCCA 
TCCAAGCAGT CCAGCACGGA AAGCATAACA GAAGAATTTT ACAGGTATAT GCTAAAAGAA 
ATAGAAAAGG AAAATAAAGA TAATGCATCT TCCCCTCGGA ATTCAAAGGA CTGGTGTGGC 
AGTTTGCTAC CACCCTCTCT GCGATCACCT TTTTGCTTTA GACAGTCCTC AGTGCCTGAT 
GGCAGATCAT CAGGTTCTAG ACTAACAGTT AACATCCCAG TCAAGGCAAA TTCATTAGAT 
GGATTTGCCC AACATCACCA GGATTCCTTA AGTGTACAGC CAGTCAGTAC TGTGGCTTCT 
TCAGGTCTTT GCAAGTCTGA CTCATGCCTG TACCAAAGAT GCAAGACTGA CCAGATAACA 
GATATGTTGA TTCACGAGAC GTGGGCAAGC TCTATTGAAT CTCTAATGCG CAAAAACAAA 
ATCATAACAG ATGAGGCAGA GGTTGCAGAG GTAGATCATT TTCACAGTGA TTCCCCACCA 
CATGTGCAAC AGTATGCAAA CAGACTGGCT GCAAATATTG TTGAAAGTGG TAAAAATTTA 
ATTGTTGTCC AGCAGGATGC CTTTGATTCT GCAAGCCAAG AACACATGCT GGAAAGCAAA 
CATCCCCAGA GTGCAACTCA GATTCAAGTA GAACCCAAAA GGAGTGGAAT AAATTTGGAT 
GAGCATATGA AGAACCCTGA ATGCCTCCCT GCAGGTCAGC ACAGGGAAGT GCCTTTAATT 
CAGATAGAAA CTGATCAACG AGACGAGTCA GATAAAGACT CTGAATCCTT AACTTTACAT 
GAGCTTTCTG AAAAGGAGCA TCAAGGCAAG GAAAAGCCTC CAGAAGCTTA TGCTGGGAAG 
CACATGGTTT CCAGTACCCT ACTGAATAGC AAAAGCAGTC AGAGCAAACC AGATGCTGAA 
ATAACGGGAG AGACAAAAGC AGCTGAAGAA TTTCCAACAC ACCTCAGCAG CAGTGAAGAA 
AGCACTGGCA GCTGGTCCCA GCTAGCCAAT GATGAAGACA ATCCTGATGA CACAAGTAGC 
TACTTACAAC TCAGCGAACG ATCTCTGAGC AATGGCAACA GCAGTACAAC TAGCAGTCTT 
GGCATTATGG ACCTGGAAAT TTATCAGGAG AACGTGCCAC CTTCTCCTAT GATTAATGAC 
TTAGTAGAAG AAAAGGTTTT CCTTAAAGAA CAGACAGATA ATACAGAGGA AAGTACTTCT 
GGGCTCTCAG TGGGAACAGC CAATTATCAG AAAGAACTCC TGGTGATTAA TTTTGATCTG 
GAACCAGAAT GCCCCAACGC AGAGCTGCGA GCCACCCTAC AGTGGATAGC TGCTTCTGAA 
CTTGGAATCC CAACCATCTA TTTTAAAAAA TCTCAGGAAA ACAGAATTGA AAAGTTTTTA 
GATGTTGTGC AGCTAGTTCA ACGGAAGTCC TGGAAAGTGG GTGACATCTT TCATGCTGTG 
GTGCAGTACT GCAAACTCAG TGAGGAGCGC AAAGAGAAGA CACCAAGCCT GTTCGATTGG 
CTCCTTGAAC TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_015276892.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132378.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X5, mRNA.

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CloneID	OGa39666
Clone ID Related Accession (Same CDS sequence)	XM_015276892.1 , XM_015276893.1 , XM_015276893.2 , XM_015276892.2
Accession Version	XM_015276892.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG CAATGGCAAC 
AGCAGTACAA CTAGCAGTCT TGGCATTATG GACCTGGAAA TTTATCAGGA GAACGTGCCA 
CCTTCTCCTA TGATTAATGA CTTAGTAGAA GAAAAGGTTT TCCTTAAAGA ACAGACAGAT 
AATACAGAGG AAAGTACTTC TGGGCTCTCA GTGGGAACAG CCAATTATCA GAAAGAACTC 
CTGGTGATTA ATTTTGATCT GGAACCAGAA TGCCCCAACG CAGAGCTGCG AGCCACCCTA 
CAGTGGATAG CTGCTTCTGA ACTTGGAATC CCAACCATCT ATTTTAAAAA ATCTCAGGAA 
AACAGAATTG AAAAGTTTTT AGATGTTGTG CAGCTAGTTC AACGGAAGTC CTGGAAAGTG 
GGTGACATCT TTCATGCTGT GGTGCAGTAC TGCAAACTCA GTGAGGAGCG CAAAGAGAAG 
ACACCAAGCC TGTTCGATTG GCTCCTTGAA CTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132378.1
CDS	116..5134
Translation	MQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTVAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENVPPSPMINDLVEEKVFLKEQTDNTEESTSGLSVGTANYQKELLVINFDLEPECPNAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFHAVVQYCKLSEERKEKTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_015276892.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276892.1

ORF Insert Sequence:

ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG CAATGGCAAC 
AGCAGTACAA CTAGCAGTCT TGGCATTATG GACCTGGAAA TTTATCAGGA GAACGTGCCA 
CCTTCTCCTA TGATTAATGA CTTAGTAGAA GAAAAGGTTT TCCTTAAAGA ACAGACAGAT 
AATACAGAGG AAAGTACTTC TGGGCTCTCA GTGGGAACAG CCAATTATCA GAAAGAACTC 
CTGGTGATTA ATTTTGATCT GGAACCAGAA TGCCCCAACG CAGAGCTGCG AGCCACCCTA 
CAGTGGATAG CTGCTTCTGA ACTTGGAATC CCAACCATCT ATTTTAAAAA ATCTCAGGAA 
AACAGAATTG AAAAGTTTTT AGATGTTGTG CAGCTAGTTC AACGGAAGTC CTGGAAAGTG 
GGTGACATCT TTCATGCTGT GGTGCAGTAC TGCAAACTCA GTGAGGAGCG CAAAGAGAAG 
ACACCAAGCC TGTTCGATTG GCTCCTTGAA CTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_015276893.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132379.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X6, mRNA.

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CloneID	OGa39666
Clone ID Related Accession (Same CDS sequence)	XM_015276892.1 , XM_015276893.1 , XM_015276893.2 , XM_015276892.2
Accession Version	XM_015276893.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG CAATGGCAAC 
AGCAGTACAA CTAGCAGTCT TGGCATTATG GACCTGGAAA TTTATCAGGA GAACGTGCCA 
CCTTCTCCTA TGATTAATGA CTTAGTAGAA GAAAAGGTTT TCCTTAAAGA ACAGACAGAT 
AATACAGAGG AAAGTACTTC TGGGCTCTCA GTGGGAACAG CCAATTATCA GAAAGAACTC 
CTGGTGATTA ATTTTGATCT GGAACCAGAA TGCCCCAACG CAGAGCTGCG AGCCACCCTA 
CAGTGGATAG CTGCTTCTGA ACTTGGAATC CCAACCATCT ATTTTAAAAA ATCTCAGGAA 
AACAGAATTG AAAAGTTTTT AGATGTTGTG CAGCTAGTTC AACGGAAGTC CTGGAAAGTG 
GGTGACATCT TTCATGCTGT GGTGCAGTAC TGCAAACTCA GTGAGGAGCG CAAAGAGAAG 
ACACCAAGCC TGTTCGATTG GCTCCTTGAA CTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132379.1
CDS	134..5152
Translation	MQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTVAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENVPPSPMINDLVEEKVFLKEQTDNTEESTSGLSVGTANYQKELLVINFDLEPECPNAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFHAVVQYCKLSEERKEKTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_015276893.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276893.1

ORF Insert Sequence:

ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG CAATGGCAAC 
AGCAGTACAA CTAGCAGTCT TGGCATTATG GACCTGGAAA TTTATCAGGA GAACGTGCCA 
CCTTCTCCTA TGATTAATGA CTTAGTAGAA GAAAAGGTTT TCCTTAAAGA ACAGACAGAT 
AATACAGAGG AAAGTACTTC TGGGCTCTCA GTGGGAACAG CCAATTATCA GAAAGAACTC 
CTGGTGATTA ATTTTGATCT GGAACCAGAA TGCCCCAACG CAGAGCTGCG AGCCACCCTA 
CAGTGGATAG CTGCTTCTGA ACTTGGAATC CCAACCATCT ATTTTAAAAA ATCTCAGGAA 
AACAGAATTG AAAAGTTTTT AGATGTTGTG CAGCTAGTTC AACGGAAGTC CTGGAAAGTG 
GGTGACATCT TTCATGCTGT GGTGCAGTAC TGCAAACTCA GTGAGGAGCG CAAAGAGAAG 
ACACCAAGCC TGTTCGATTG GCTCCTTGAA CTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_015276893.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132379.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa39666
Clone ID Related Accession (Same CDS sequence)	XM_015276892.1 , XM_015276893.1 , XM_015276893.2 , XM_015276892.2
Accession Version	XM_015276893.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276893.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG CAATGGCAAC 
AGCAGTACAA CTAGCAGTCT TGGCATTATG GACCTGGAAA TTTATCAGGA GAACGTGCCA 
CCTTCTCCTA TGATTAATGA CTTAGTAGAA GAAAAGGTTT TCCTTAAAGA ACAGACAGAT 
AATACAGAGG AAAGTACTTC TGGGCTCTCA GTGGGAACAG CCAATTATCA GAAAGAACTC 
CTGGTGATTA ATTTTGATCT GGAACCAGAA TGCCCCAACG CAGAGCTGCG AGCCACCCTA 
CAGTGGATAG CTGCTTCTGA ACTTGGAATC CCAACCATCT ATTTTAAAAA ATCTCAGGAA 
AACAGAATTG AAAAGTTTTT AGATGTTGTG CAGCTAGTTC AACGGAAGTC CTGGAAAGTG 
GGTGACATCT TTCATGCTGT GGTGCAGTAC TGCAAACTCA GTGAGGAGCG CAAAGAGAAG 
ACACCAAGCC TGTTCGATTG GCTCCTTGAA CTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132379.1
CDS	166..5184
Translation	MQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTVAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENVPPSPMINDLVEEKVFLKEQTDNTEESTSGLSVGTANYQKELLVINFDLEPECPNAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFHAVVQYCKLSEERKEKTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_015276893.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276893.2

ORF Insert Sequence:

ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG CAATGGCAAC 
AGCAGTACAA CTAGCAGTCT TGGCATTATG GACCTGGAAA TTTATCAGGA GAACGTGCCA 
CCTTCTCCTA TGATTAATGA CTTAGTAGAA GAAAAGGTTT TCCTTAAAGA ACAGACAGAT 
AATACAGAGG AAAGTACTTC TGGGCTCTCA GTGGGAACAG CCAATTATCA GAAAGAACTC 
CTGGTGATTA ATTTTGATCT GGAACCAGAA TGCCCCAACG CAGAGCTGCG AGCCACCCTA 
CAGTGGATAG CTGCTTCTGA ACTTGGAATC CCAACCATCT ATTTTAAAAA ATCTCAGGAA 
AACAGAATTG AAAAGTTTTT AGATGTTGTG CAGCTAGTTC AACGGAAGTC CTGGAAAGTG 
GGTGACATCT TTCATGCTGT GGTGCAGTAC TGCAAACTCA GTGAGGAGCG CAAAGAGAAG 
ACACCAAGCC TGTTCGATTG GCTCCTTGAA CTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_015276892.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132378.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X5, mRNA.

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CloneID	OGa39666
Clone ID Related Accession (Same CDS sequence)	XM_015276892.1 , XM_015276893.1 , XM_015276893.2 , XM_015276892.2
Accession Version	XM_015276892.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276892.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG CAATGGCAAC 
AGCAGTACAA CTAGCAGTCT TGGCATTATG GACCTGGAAA TTTATCAGGA GAACGTGCCA 
CCTTCTCCTA TGATTAATGA CTTAGTAGAA GAAAAGGTTT TCCTTAAAGA ACAGACAGAT 
AATACAGAGG AAAGTACTTC TGGGCTCTCA GTGGGAACAG CCAATTATCA GAAAGAACTC 
CTGGTGATTA ATTTTGATCT GGAACCAGAA TGCCCCAACG CAGAGCTGCG AGCCACCCTA 
CAGTGGATAG CTGCTTCTGA ACTTGGAATC CCAACCATCT ATTTTAAAAA ATCTCAGGAA 
AACAGAATTG AAAAGTTTTT AGATGTTGTG CAGCTAGTTC AACGGAAGTC CTGGAAAGTG 
GGTGACATCT TTCATGCTGT GGTGCAGTAC TGCAAACTCA GTGAGGAGCG CAAAGAGAAG 
ACACCAAGCC TGTTCGATTG GCTCCTTGAA CTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132378.1
CDS	161..5179
Translation	MQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTVAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSNGNSSTTSSLGIMDLEIYQENVPPSPMINDLVEEKVFLKEQTDNTEESTSGLSVGTANYQKELLVINFDLEPECPNAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFHAVVQYCKLSEERKEKTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_015276892.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276892.2

ORF Insert Sequence:

ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG CAATGGCAAC 
AGCAGTACAA CTAGCAGTCT TGGCATTATG GACCTGGAAA TTTATCAGGA GAACGTGCCA 
CCTTCTCCTA TGATTAATGA CTTAGTAGAA GAAAAGGTTT TCCTTAAAGA ACAGACAGAT 
AATACAGAGG AAAGTACTTC TGGGCTCTCA GTGGGAACAG CCAATTATCA GAAAGAACTC 
CTGGTGATTA ATTTTGATCT GGAACCAGAA TGCCCCAACG CAGAGCTGCG AGCCACCCTA 
CAGTGGATAG CTGCTTCTGA ACTTGGAATC CCAACCATCT ATTTTAAAAA ATCTCAGGAA 
AACAGAATTG AAAAGTTTTT AGATGTTGTG CAGCTAGTTC AACGGAAGTC CTGGAAAGTG 
GGTGACATCT TTCATGCTGT GGTGCAGTAC TGCAAACTCA GTGAGGAGCG CAAAGAGAAG 
ACACCAAGCC TGTTCGATTG GCTCCTTGAA CTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_015276894.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132380.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X7, mRNA.

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CloneID	OGa39667
Clone ID Related Accession (Same CDS sequence)	XM_015276894.1 , XM_015276894.2
Accession Version	XM_015276894.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCC CGGCCACCCG CCGCCACCCG GCGCTTCGCA GCAACTTTGA ATCTCCACTG 
ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132380.1
CDS	587..5203
Translation	MFRPATRRHPALRSNFESPLMQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTVAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSF

Target ORF information:

RefSeq Version	XM_015276894.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276894.1

ORF Insert Sequence:

ATGTTCCGCC CGGCCACCCG CCGCCACCCG GCGCTTCGCA GCAACTTTGA ATCTCCACTG 
ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_015276894.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132380.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X7, mRNA.

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CloneID	OGa39667
Clone ID Related Accession (Same CDS sequence)	XM_015276894.1 , XM_015276894.2
Accession Version	XM_015276894.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276894.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCC CGGCCACCCG CCGCCACCCG GCGCTTCGCA GCAACTTTGA ATCTCCACTG 
ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132380.1
CDS	276..4892
Translation	MFRPATRRHPALRSNFESPLMQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTVAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSF

Target ORF information:

RefSeq Version	XM_015276894.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276894.2

ORF Insert Sequence:

ATGTTCCGCC CGGCCACCCG CCGCCACCCG GCGCTTCGCA GCAACTTTGA ATCTCCACTG 
ATGCAAGAAG TCCCAGAGCA CCAAGGCAAC AGCACAGATA GTTCGGGAAG CAGTTTGGGA 
AGCACTGTCA CAGCATGTAA AAAGATCCTC TGCAGTAATA GCCTGCTAGA ATCAACAGAC 
TACTGGCTGC AGAATCAGAG GACACCATGC CAAATTGGCT TTCTGGAAGA CAAGTCAGAA 
AGCAACTGTG CCTCAGTTTG TTTTGTGAAT TTAGATGCAA ACAGAGACGA TTGCAGCAAT 
GAACAAGTGA AACAGAAATT GATCAGCGTC TCTCCAAATC TCCCTAAACT CATCGGATCT 
ATGAATGTAC AACCACCAAA GGAAAATGAA ATAGTCCTAC TGAGCGGATT AACGTCAGGA 
AATCTTCAGG CTGATTATGA AGTTCCCCAG TGTCCTTGGC AAGCAGATGT CTGCTTGGTT 
CAGTGTGCGA GGGGAGACAG GAAGAACAGT GCAAGCTGCA TCATCTTTGA AATAAACAAG 
TTTCTGATTG GGCTTGAGCT TGTTCAGGAG AGACAGCTGC AGATAGAAGC TCATGTCTCA 
AAGCCTGAGG ACGACACAAA CTGCTCAGTG TCCTCAATAG AAGAAGATTT CCTCACAGCA 
TCTGAGCATC TCGAGGAGGA AAACGAGGCT GATGAATATA AAACTGTAGC CCACGAAAAA 
ATAAGTGTTT CAGAAGCATC TTCTGATGTC AGAAAAAATA AAGGAAAAGG ATTTGAAAGT 
CTCCACTACA GAAAAGCCAG GTTGCCATTA ATTCTTGAAG GGAATTGTGT TAATGAAGAT 
AATACAGCTA GTAAAGTCTC TGAATCTGTG CACGTTGTGG CTGAAGATGG CATCTCACAA 
CCTGAAGATA AGCCAGAGCA GGAAATCCTG TGTCAAGAGG AATTAGTGGG AAGAGATACA 
TCATCCCTCA GTACTGAGGA TTTGACTAAC AATGTTGAAA ATTCCTGCTC AGTGAATGTG 
TTAGATGTGT CTTTAACCAA AATGGCTAAA GAGGAGCTGG GGCCTCTTTA TGGCTCTACA 
GCAAAACCCA ACAGTAAGGC AGAGGTAGAG GATTGTGCAG GTGTTAGGAA AAGCGACCCT 
CCCTTGCAAA ACGAGCAAGC AGCTACAGGT CAGTACGCTA CAAATCTAGC AGAATCTGTT 
CTGCAAGATG CATTCATCAG ACTATCACAG TCTCAACCCA CTTTCAGTGA GGAGGCTGCA 
GTCAGCATCT CCGTAGGAAG CTCCTGCAAG TCAAAAGATG CATCTGCTTC CCGATCGTGG 
AATGAACTTC CAAAGATTGT CATAGTGCAA AGTCCAGACA GCAGTGAGAA TGTGCCAGAC 
TGGCCAGGGT CTGCCTTCCC AAACCTGTGC CACTGGACTG AAACAGAAAG TTCTGAAGTT 
TCAGATTACT TTGAAGAAGA CCATTCAAAT GGACGCAGCC AGAGTGCACT GGAGATGGCT 
CTGGCTTGTG CAGCAACTGT CATTGGAACC ATTTCAAGTC CACAGGCAGC AGAAAAATTC 
AGAAGGGATC AAGAAGCCAC AGACTCTAAA AGCGAAACAG TTGATAACGA AGATCTACAC 
GCAGATTCTT CACAGCTACT TGAAGACTGT ACTAGCACAG ATTATTCATT TCCATCTGCA 
CTGTGTGGCA TGACTCAAGT AGCAAGTGCT GTAGCAGTCT GCGGTCTGGG GGAAACAAAG 
GAAGAGAAGT ACCCTACAAC TTCAAGCGGG CTGCTGTCTG CAGCTCAGAC TTCTGCAGCC 
ATTACTCTTC ATTGTAGCAT AGCTGTAGGA AGCAGCATGG AAAAGCTGAA TGATAGCATT 
GCAGAGGCCC TACTCAAAGA GGCATCAATA ATTTTGACAA AACCGAATAC ATACAAAAAT 
GTAGGGCATT TTATGGAATC CATAAACGGA AGAATTATTG AAACAGCAGC AAGGCCACGG 
ATTCCACACT CCGATGAAGT AATCAGAGAT GAACTGGCAC AAAATTTATC CAATATTATT 
CTACGACATT CTATTGAAGA GGTCAGGAAG AAAAGAGAGC TACAGTCCTG TTCAGAAAAC 
AGCTCAAGTA CACAAGACAT TTTCATGGAG ACTGCCAATG AGTTGCTTTT TAATGTGATA 
TATTTCACTT GCAAGAAGAT GAATGACATA AGACAAGTTG AAGAGTGTTC TCCTCTCTTT 
TCTGAAGGCT TGAAAGGCAA GAAGGTAACA AGAGTAGAAG GATGGCCAAC ACAGGCAACA 
GCATGTGAAA CATCACACAG ACCCCTTGAT CATTCTGCTA TTAAGCCATT TGGTACATCC 
TACAGTGCTA GCACAGATAG TGAAAATGGT ATCAGCACGA AGAAAATTAA CATGAGAGAT 
GTAAATAGCA ATGAAAACGC CACAGTGAAT TCAGAGCTAA CAAGTAGAGT CATTGGGCAT 
AATGCACTTG TTGCAAAAAC ATCTCCCAAG AAAAGATACC TGAAAAGAAC CACACGAGAC 
TGCTACAAAT CCCCACATCA GAATAACATT CAGAAGAAGA GAGATTACAG ATCATTTTCA 
GACAGAGAAA GTACCTTTGC AAACAATGAA TGCAGGCACG GTGTTCAAGA ACAACTGTCT 
TCCAGCGCTG TCGTGAATGC AGAAAACCAG GCCAAACATA AGTGTGATGC TGTGCTAAGT 
GATGAAGTCC AAGTTAGCTT GTCTTTGTTA GGAAACCATG TCTTACTTCC TTCTCAGCCT 
GTGCTACAGG TGAAAAATTC AAGAGACAAA TACTGTATAA CAGATTTTGC AGAAGAATTG 
GCAGAAACAG TGGTCTCTAT GGCAACAGAA ATAGCTGCCA TTTGTCTTGA AAACTCAAAT 
GGAAAGCAGC CCTGGTTCTG TGCATGGAAG AGAGGCAATG AATATCTGGT GACCCAGAGT 
ATATCATGCA GAACTATGAA AAGGAAAAAG GAAACCCATG CTAATGGCTC AGTTGTTCGG 
AAACACAGGG CACCTCGACT GAGTGAGATC AAAAGAAAGA CAGATGAGCA TCCTGAGCTA 
AAGGAAAGGT TGATGAATCG AGTAGTAGAT GAATCTATAA ACCTTGAGGA CACACCAGAT 
TCTGTCAATA TATTTGCAAA TGAAGTGGCT GCAAAGATCA TGAACCTCAC TGAACTCTCC 
ATGGTTGACA GTATCTGGCA AGGTCCAAAC CATCCCAGGA ACAGGCTGCA CTGTGAAAGA 
TGGAGCCGAG CCAAGGCCTC AAGCTGTGAG AGCATACCAG AGGAGGACAT GGATTCCAAA 
GCCTCTGCCA ATACTCTAGG CCTAATGAAT GCCCTTGGTC AGCCTGTGAG CCAGACAAGT 
TCCGTCTCAA AGCAGTCTAG TTGTGAAAGC ATTACAGATG AGTTTTCAAG ATTTATGGTG 
AACCAAATGG AAAATGAGGG AAGAGGCTTT GATTTATTAC TAGATTATTA TGCAGGAAAA 
AATGCAAACA ACATCTTAAC GTCGGCTTTG CAACAGGTTG CTAAGAAAAA TGGTCATCTT 
AATGTACGGC CAAGTTGCCC ATCCAAGCAG TCCAGCACGG AAAGCATAAC AGAAGAATTT 
TACAGGTATA TGCTAAAAGA AATAGAAAAG GAAAATAAAG ATAATGCATC TTCCCCTCGG 
AATTCAAAGG ACTGGTGTGG CAGTTTGCTA CCACCCTCTC TGCGATCACC TTTTTGCTTT 
AGACAGTCCT CAGTGCCTGA TGGCAGATCA TCAGGTTCTA GACTAACAGT TAACATCCCA 
GTCAAGGCAA ATTCATTAGA TGGATTTGCC CAACATCACC AGGATTCCTT AAGTGTACAG 
CCAGTCAGTA CTGTGGCTTC TTCAGGTCTT TGCAAGTCTG ACTCATGCCT GTACCAAAGA 
TGCAAGACTG ACCAGATAAC AGATATGTTG ATTCACGAGA CGTGGGCAAG CTCTATTGAA 
TCTCTAATGC GCAAAAACAA AATCATAACA GATGAGGCAG AGGTTGCAGA GGTAGATCAT 
TTTCACAGTG ATTCCCCACC ACATGTGCAA CAGTATGCAA ACAGACTGGC TGCAAATATT 
GTTGAAAGTG GTAAAAATTT AATTGTTGTC CAGCAGGATG CCTTTGATTC TGCAAGCCAA 
GAACACATGC TGGAAAGCAA ACATCCCCAG AGTGCAACTC AGATTCAAGT AGAACCCAAA 
AGGAGTGGAA TAAATTTGGA TGAGCATATG AAGAACCCTG AATGCCTCCC TGCAGGTCAG 
CACAGGGAAG TGCCTTTAAT TCAGATAGAA ACTGATCAAC GAGACGAGTC AGATAAAGAC 
TCTGAATCCT TAACTTTACA TGAGCTTTCT GAAAAGGAGC ATCAAGGCAA GGAAAAGCCT 
CCAGAAGCTT ATGCTGGGAA GCACATGGTT TCCAGTACCC TACTGAATAG CAAAAGCAGT 
CAGAGCAAAC CAGATGCTGA AATAACGGGA GAGACAAAAG CAGCTGAAGA ATTTCCAACA 
CACCTCAGCA GCAGTGAAGA AAGCACTGGC AGCTGGTCCC AGCTAGCCAA TGATGAAGAC 
AATCCTGATG ACACAAGTAG CTACTTACAA CTCAGCGAAC GATCTCTGAG TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( NM_001177532.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001171003.1 Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), mRNA.

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CloneID	OGa24472
Clone ID Related Accession (Same CDS sequence)	NM_001177532.3
Accession Version	NM_001177532.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-25
Organism	Gallus gallus(chicken)
Product	A-kinase anchor protein SPHKAP
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AADN05000526.1. On Feb 10, 2016 this sequence version replaced NM_001177532.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201360, SAMEA2201362 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGGCC GCCCCCCGCC CAGCAACTTT GAATCTCCAC TGATGCAAGA AGTCCCAGAG 
CACCAAGGCA ACAGCACAGA TAGTTCGGGA AGCAGTTTGG GAAGCACTGT CACAGCATGT 
AAAAAGATCC TCTGCAGTAA TAGCCTGCTA GAATCAACAG ACTACTGGCT GCAGAATCAG 
AGGACACCAT GCCAAATTGG CTTTCTGGAA GACAAGTCAG AAAGCAACTG TGCCTCAGTT 
TGTTTTGTGA ATTTAGATGC AAACAGAGAC GATTGCAGCA ATGAACAAGT GAAACAGAAA 
TTGATCAGCG TCTCTCCAAA TCTCCCTAAA CTCATCGGAT CTATGAATGT ACAACCACCA 
AAGGAAAATG AAATAGTCCT ACTGAGCGGA TTAACGTCAG GAAATCTTCA GGCTGATTAT 
GAAGTTCCCC AGTGTCCTTG GCAAGCAGAT GTCTGCTTGG TTCAGTGTGC GAGGGGAGAC 
AGGAAGAACA GTGCAAGCTG CATCATCTTT GAAATAAACA AGTTTCTGAT TGGGCTTGAG 
CTTGTTCAGG AGAGACAGCT GCAGATAGAA GCTCATGTCT CAAAGCCTGA GGACGACACA 
AACTGCTCAG TGTCCTCAAT AGAAGAAGAT TTCCTCACAG CATCTGAGCA TCTCGAGGAG 
GAAAACGAGG CTGATGAATA TAAAACTGCC CACGAAAAAA TAAGTGTTTC AGAAGCATCT 
TCTGATGTCA GAAAAAATAA AGGAAAAGGA TTTGAAAGTC TCCACTACAG AAAAGCCAGG 
TTGCCATTAA TTCTTGAAGG GAATTGTGTT AATGAAGATA ATACAGCTAG TAAAGTCTCT 
GAATCTGTGC ACGTTGTGGC TGAAGATGGC ATCTCACAAC CTGAAGATAA GCCAGAGCAG 
GAAATCCTGT GTCAAGAGGA ATTAGTGGGA AGAGATACAT CATCCCTCAG TACTGAGGAT 
TTGACTAACA ATGTTGAAAA TTCCTGCTCA GTGAATGTGT TAGATGTGTC TTTAACCAAA 
ATGGCTAAAG AGGAGCTGGG GCCTCTTTAT GGCTCTACAG CAAAACCCAA CAGTAAGGCA 
GAGGTAGAGG ATTGTGCAGG TGTTAGGAAA AGCGACCCTC CCTTGCAAAA CGAGCAAGCA 
GCTACAGGTC AGTACGCTAC AAATCTAGCA GAATCTGTTC TGCAAGATGC ATTCATCAGA 
CTATCACAGT CTCAACCCAC TTTCAGTGAG GAGGCTGCAG TCAGCATCTC CGTAGGAAGC 
TCCTGCAAGT CAAAAGATGC ATCTGCTTCC CGATCGTGGA ATGAACTTCC AAAGATTGTC 
ATAGTGCAAA GTCCAGACAG CAGTGAGAAT GTGCCAGACT GGCCAGGGTC TGCCTTCCCA 
AACCTGTGCC ACTGGACTGA AACAGAAAGT TCTGAAGTTT CAGATTACTT TGAAGAAGAC 
CATTCAAATG GACGCAGCCA GAGTGCACTG GAGATGGCTC TGGCTTGTGC AGCAACTGTC 
ATTGGAACCA TTTCAAGTCC ACAGGCAGCA GAAAAATTCA GAAGGGATCA AGAAGCCACA 
GACTCTAAAA GCGAAACAGT TGATAACGAA GATCTACACG CAGATTCTTC ACAGCTACTT 
GAAGACTGTA CTAGCACAGA TTATTCATTT CCATCTGCAC TGTGTGGCAT GACTCAAGTA 
GCAAGTGCTG TAGCAGTCTG CGGTCTGGGG GAAACAAAGG AAGAGAAGTA CCCTACAACT 
TCAAGCGGGC TGCTGTCTGC AGCTCAGACT TCTGCAGCCA TTACTCTTCA TTGTAGCATA 
GCTGTAGGAA GCAGCATGGA AAAGCTGAAT GATAGCATTG CAGAGGCCCT ACTCAAAGAG 
GCATCAATAA TTTTGACAAA ACCGAATACA TACAAAAATG TAGGGCATTT TATGGAATCC 
ATAAACGGAA GAATTATTGA AACAGCAGCA AGGCCACGGA TTCCACACTC CGATGAAGTA 
ATCAGAGATG AACTGGCACA AAATTTATCC AATATTATTC TACGACATTC TATTGAAGAG 
GTCAGGAAGA AAAGAGAGCT ACAGTCCTGT TCAGAAAACA GCTCAAGTAC ACAAGACATT 
TTCATGGAGA CTGCCAATGA GTTGCTTTTT AATGTGATAT ATTTCACTTG CAAGAAGATG 
AATGACATAA GACAAGTTGA AGAGTGTTCT CCTCTCTTTT CTGAAGGCTT GAAAGGCAAG 
AAGGTAACAA GAGTAGAAGG ATGGCCAACA CAGGCAACAG CATGTGAAAC ATCACACAGA 
CCCCTTGATC ATTCTGCTAT TAAGCCATTT GGTACATCCT ACAGTGCTAG CACAGATAGT 
GAAAATGGTA TCAGCACGAA GAAAATTAAC ATGAGAGATG TAAATAGCAA TGAAAACGCC 
ACAGTGAATT CAGAGCTAAC AAGTAGAGTC ATTGGGCATA ATGCACTTGT TGCAAAAACA 
TCTCCCAAGA AAAGATACCT GAAAAGAACC ACACGAGACT GCTACAAATC CCCACATCAG 
AATAACATTC AGAAGAAGAG AGATTACAGA TCATTTTCAG ACAGAGAAAG TACCTTTGCA 
AACAATGAAT GCAGGCACGG TGTTCAAGAA CAACTGTCTT CCAGCGCTGT CGTGAATGCA 
GAAAACCAGG CCAAACATAA GTGTGATGCT GTGCTAAGTG ATGAAGTCCA AGTTAGCTTG 
TCTTTGTTAG GAAACCATGT CTTACTTCCT TCTCAGCCTG TGCTACAGGT GAAAAATTCA 
AGAGACAAAT ACTGTATAAC AGATTTTGCA GAAGAATTGG CAGAAACAGT GGTCTCTATG 
GCAACAGAAA TAGCTGCCAT TTGTCTTGAA AACTCAAATG GAAAGCAGCC CTGGTTCTGT 
GCATGGAAGA GAGGCAATGA ATATCTGGTG ACCCAGAGTA TATCATGCAG AACTATGAAA 
AGGAAAAAGG AAACCCATGC TAATGGCTCA GTTGTTCGGA AACACAGGGC ACCTCGACTG 
AGTGAGATCA AAAGAAAGAC AGATGAGCAT CCTGAGCTAA AGGAAAGGTT GATGAATCGA 
GTAGTAGATG AATCTATAAA CCTTGAGGAC ACACCAGATT CTGTCAATAT ATTTGCAAAT 
GAAGTGGCTG CAAAGATCAT GAACCTCACT GAACTCTCCA TGGTTGACAG TATCTGGCAA 
GGTCCAAACC ATCCCAGGAA CAGGCTGCAC TGTGAAAGAT GGAGCCGAGC CAAGGCCTCA 
AGCTGTGAGA GCATACCAGA GGAGGACATG GATTCCAAAG CCTCTGCCAA TACTCTAGGC 
CTAATGAATG CCCTTGGTCA GCCTGTGAGC CAGACAAGTT CCGTCTCAAA GCAGTCTAGT 
TGTGAAAGCA TTACAGATGA GTTTTCAAGA TTTATGGTGA ACCAAATGGA AAATGAGGGA 
AGAGGCTTTG ATTTATTACT AGATTATTAT GCAGGAAAAA ATGCAAACAA CATCTTAACG 
TCGGCTTTGC AACAGGTTGC TAAGAAAAAT GGTCATCTTA ATGTACGGCC AAGTTGCCCA 
TCCAAGCAGT CCAGCACGGA AAGCATAACA GAAGAATTTT ACAGGTATAT GCTAAAAGAA 
ATAGAAAAGG AAAATAAAGA TAATGCATCT TCCCCTCGGA ATTCAAAGGA CTGGTGTGGC 
AGTTTGCTAC CACCCTCTCT GCGATCACCT TTTTGCTTTA GACAGTCCTC AGTGCCTGAT 
GGCAGATCAT CAGGTTCTAG ACTAACAGTT AACATCCCAG TCAAGGCAAA TTCATTAGAT 
GGATTTGCCC AACATCACCA GGATTCCTTA AGTGTACAGC CAGTCAGTAC TGTGGCTTCT 
TCAGGTCTTT GCAAGTCTGA CTCATGCCTG TACCAAAGAT GCAAGACTGA CCAGATAACA 
GATATGTTGA TTCACGAGAC GTGGGCAAGC TCTATTGAAT CTCTAATGCG CAAAAACAAA 
ATCATAACAG ATGAGGCAGA GGTTGCAGAG GTAGATCATT TTCACAGTGA TTCCCCACCA 
CATGTGCAAC AGTATGCAAA CAGACTGGCT GCAAATATTG TTGAAAGTGG TAAAAATTTA 
ATTGTTGTCC AGCAGGATGC CTTTGATTCT GCAAGCCAAG AACACATGCT GGAAAGCAAA 
CATCCCCAGA GTGCAACTCA GATTCAAGTA GAACCCAAAA GGAGTGGAAT AAATTTGGAT 
GAGCATATGA AGAACCCTGA ATGCCTCCCT GCAGGTCAGC ACAGGGAAGT GCCTTTAATT 
CAGATAGAAA CTGATCAACG AGACGAGTCA GATAAAGACT CTGAATCCTT AACTTTACAT 
GAGCTTTCTG AAAAGGAGCA TCAAGGCAAG GAAAAGCCTC CAGAAGCTTA TGCTGGGAAG 
CACATGGTTT CCAGTACCCT ACTGAATAGC AAAAGCAGTC AGAGCAAACC AGATGCTGAA 
ATAACGGGAG AGACAAAAGC AGCTGAAGAA TTTCCAACAC ACCTCAGCAG CAGTGAAGAA 
AGCACTGGCA GCTGGTCCCA GCTAGCCAAT GATGAAGACA ATCCTGATGA CACAAGTAGC 
TACTTACAAC TCAGCGAACG ATCTCTGAGT GACTTAGTAG AAGAAAAGGT TTTCCTTAAA 
GAACAGACAG ATAATACAGA GGAAAGTACT TCTGGGCTCT CAGTGGGAAC AGCCAATTAT 
CAGAAAGAAC TCCTGGTGAT TAATTTTGAT CTGGAACCAG AATGCCCCAA CGCAGAGCTG 
CGAGCCACCC TACAGTGGAT AGCTGCTTCT GAACTTGGAA TCCCAACCAT CTATTTTAAA 
AAATCTCAGG AAAACAGAAT TGAAAAGTTT TTAGATGTTG TGCAGCTAGT TCAACGGAAG 
TCCTGGAAAG TGGGTGACAT CTTTCATGCT GTGGTGCAGT ACTGCAAACT CAGTGAGGAG 
CGCAAAGAGA AGACACCAAG CCTGTTCGAT TGGCTCCTTG AACTGGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171003.1
CDS	129..5099
Translation	MAGRPPPSNFESPLMQEVPEHQGNSTDSSGSSLGSTVTACKKILCSNSLLESTDYWLQNQRTPCQIGFLEDKSESNCASVCFVNLDANRDDCSNEQVKQKLISVSPNLPKLIGSMNVQPPKENEIVLLSGLTSGNLQADYEVPQCPWQADVCLVQCARGDRKNSASCIIFEINKFLIGLELVQERQLQIEAHVSKPEDDTNCSVSSIEEDFLTASEHLEEENEADEYKTAHEKISVSEASSDVRKNKGKGFESLHYRKARLPLILEGNCVNEDNTASKVSESVHVVAEDGISQPEDKPEQEILCQEELVGRDTSSLSTEDLTNNVENSCSVNVLDVSLTKMAKEELGPLYGSTAKPNSKAEVEDCAGVRKSDPPLQNEQAATGQYATNLAESVLQDAFIRLSQSQPTFSEEAAVSISVGSSCKSKDASASRSWNELPKIVIVQSPDSSENVPDWPGSAFPNLCHWTETESSEVSDYFEEDHSNGRSQSALEMALACAATVIGTISSPQAAEKFRRDQEATDSKSETVDNEDLHADSSQLLEDCTSTDYSFPSALCGMTQVASAVAVCGLGETKEEKYPTTSSGLLSAAQTSAAITLHCSIAVGSSMEKLNDSIAEALLKEASIILTKPNTYKNVGHFMESINGRIIETAARPRIPHSDEVIRDELAQNLSNIILRHSIEEVRKKRELQSCSENSSSTQDIFMETANELLFNVIYFTCKKMNDIRQVEECSPLFSEGLKGKKVTRVEGWPTQATACETSHRPLDHSAIKPFGTSYSASTDSENGISTKKINMRDVNSNENATVNSELTSRVIGHNALVAKTSPKKRYLKRTTRDCYKSPHQNNIQKKRDYRSFSDRESTFANNECRHGVQEQLSSSAVVNAENQAKHKCDAVLSDEVQVSLSLLGNHVLLPSQPVLQVKNSRDKYCITDFAEELAETVVSMATEIAAICLENSNGKQPWFCAWKRGNEYLVTQSISCRTMKRKKETHANGSVVRKHRAPRLSEIKRKTDEHPELKERLMNRVVDESINLEDTPDSVNIFANEVAAKIMNLTELSMVDSIWQGPNHPRNRLHCERWSRAKASSCESIPEEDMDSKASANTLGLMNALGQPVSQTSSVSKQSSCESITDEFSRFMVNQMENEGRGFDLLLDYYAGKNANNILTSALQQVAKKNGHLNVRPSCPSKQSSTESITEEFYRYMLKEIEKENKDNASSPRNSKDWCGSLLPPSLRSPFCFRQSSVPDGRSSGSRLTVNIPVKANSLDGFAQHHQDSLSVQPVSTVASSGLCKSDSCLYQRCKTDQITDMLIHETWASSIESLMRKNKIITDEAEVAEVDHFHSDSPPHVQQYANRLAANIVESGKNLIVVQQDAFDSASQEHMLESKHPQSATQIQVEPKRSGINLDEHMKNPECLPAGQHREVPLIQIETDQRDESDKDSESLTLHELSEKEHQGKEKPPEAYAGKHMVSSTLLNSKSSQSKPDAEITGETKAAEEFPTHLSSSEESTGSWSQLANDEDNPDDTSSYLQLSERSLSDLVEEKVFLKEQTDNTEESTSGLSVGTANYQKELLVINFDLEPECPNAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVQRKSWKVGDIFHAVVQYCKLSEERKEKTPSLFDWLLELG

Target ORF information:

RefSeq Version	NM_001177532.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus SPHK1 interactor, AKAP domain containing (SPHKAP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177532.3

ORF Insert Sequence:

ATGGCCGGCC GCCCCCCGCC CAGCAACTTT GAATCTCCAC TGATGCAAGA AGTCCCAGAG 
CACCAAGGCA ACAGCACAGA TAGTTCGGGA AGCAGTTTGG GAAGCACTGT CACAGCATGT 
AAAAAGATCC TCTGCAGTAA TAGCCTGCTA GAATCAACAG ACTACTGGCT GCAGAATCAG 
AGGACACCAT GCCAAATTGG CTTTCTGGAA GACAAGTCAG AAAGCAACTG TGCCTCAGTT 
TGTTTTGTGA ATTTAGATGC AAACAGAGAC GATTGCAGCA ATGAACAAGT GAAACAGAAA 
TTGATCAGCG TCTCTCCAAA TCTCCCTAAA CTCATCGGAT CTATGAATGT ACAACCACCA 
AAGGAAAATG AAATAGTCCT ACTGAGCGGA TTAACGTCAG GAAATCTTCA GGCTGATTAT 
GAAGTTCCCC AGTGTCCTTG GCAAGCAGAT GTCTGCTTGG TTCAGTGTGC GAGGGGAGAC 
AGGAAGAACA GTGCAAGCTG CATCATCTTT GAAATAAACA AGTTTCTGAT TGGGCTTGAG 
CTTGTTCAGG AGAGACAGCT GCAGATAGAA GCTCATGTCT CAAAGCCTGA GGACGACACA 
AACTGCTCAG TGTCCTCAAT AGAAGAAGAT TTCCTCACAG CATCTGAGCA TCTCGAGGAG 
GAAAACGAGG CTGATGAATA TAAAACTGCC CACGAAAAAA TAAGTGTTTC AGAAGCATCT 
TCTGATGTCA GAAAAAATAA AGGAAAAGGA TTTGAAAGTC TCCACTACAG AAAAGCCAGG 
TTGCCATTAA TTCTTGAAGG GAATTGTGTT AATGAAGATA ATACAGCTAG TAAAGTCTCT 
GAATCTGTGC ACGTTGTGGC TGAAGATGGC ATCTCACAAC CTGAAGATAA GCCAGAGCAG 
GAAATCCTGT GTCAAGAGGA ATTAGTGGGA AGAGATACAT CATCCCTCAG TACTGAGGAT 
TTGACTAACA ATGTTGAAAA TTCCTGCTCA GTGAATGTGT TAGATGTGTC TTTAACCAAA 
ATGGCTAAAG AGGAGCTGGG GCCTCTTTAT GGCTCTACAG CAAAACCCAA CAGTAAGGCA 
GAGGTAGAGG ATTGTGCAGG TGTTAGGAAA AGCGACCCTC CCTTGCAAAA CGAGCAAGCA 
GCTACAGGTC AGTACGCTAC AAATCTAGCA GAATCTGTTC TGCAAGATGC ATTCATCAGA 
CTATCACAGT CTCAACCCAC TTTCAGTGAG GAGGCTGCAG TCAGCATCTC CGTAGGAAGC 
TCCTGCAAGT CAAAAGATGC ATCTGCTTCC CGATCGTGGA ATGAACTTCC AAAGATTGTC 
ATAGTGCAAA GTCCAGACAG CAGTGAGAAT GTGCCAGACT GGCCAGGGTC TGCCTTCCCA 
AACCTGTGCC ACTGGACTGA AACAGAAAGT TCTGAAGTTT CAGATTACTT TGAAGAAGAC 
CATTCAAATG GACGCAGCCA GAGTGCACTG GAGATGGCTC TGGCTTGTGC AGCAACTGTC 
ATTGGAACCA TTTCAAGTCC ACAGGCAGCA GAAAAATTCA GAAGGGATCA AGAAGCCACA 
GACTCTAAAA GCGAAACAGT TGATAACGAA GATCTACACG CAGATTCTTC ACAGCTACTT 
GAAGACTGTA CTAGCACAGA TTATTCATTT CCATCTGCAC TGTGTGGCAT GACTCAAGTA 
GCAAGTGCTG TAGCAGTCTG CGGTCTGGGG GAAACAAAGG AAGAGAAGTA CCCTACAACT 
TCAAGCGGGC TGCTGTCTGC AGCTCAGACT TCTGCAGCCA TTACTCTTCA TTGTAGCATA 
GCTGTAGGAA GCAGCATGGA AAAGCTGAAT GATAGCATTG CAGAGGCCCT ACTCAAAGAG 
GCATCAATAA TTTTGACAAA ACCGAATACA TACAAAAATG TAGGGCATTT TATGGAATCC 
ATAAACGGAA GAATTATTGA AACAGCAGCA AGGCCACGGA TTCCACACTC CGATGAAGTA 
ATCAGAGATG AACTGGCACA AAATTTATCC AATATTATTC TACGACATTC TATTGAAGAG 
GTCAGGAAGA AAAGAGAGCT ACAGTCCTGT TCAGAAAACA GCTCAAGTAC ACAAGACATT 
TTCATGGAGA CTGCCAATGA GTTGCTTTTT AATGTGATAT ATTTCACTTG CAAGAAGATG 
AATGACATAA GACAAGTTGA AGAGTGTTCT CCTCTCTTTT CTGAAGGCTT GAAAGGCAAG 
AAGGTAACAA GAGTAGAAGG ATGGCCAACA CAGGCAACAG CATGTGAAAC ATCACACAGA 
CCCCTTGATC ATTCTGCTAT TAAGCCATTT GGTACATCCT ACAGTGCTAG CACAGATAGT 
GAAAATGGTA TCAGCACGAA GAAAATTAAC ATGAGAGATG TAAATAGCAA TGAAAACGCC 
ACAGTGAATT CAGAGCTAAC AAGTAGAGTC ATTGGGCATA ATGCACTTGT TGCAAAAACA 
TCTCCCAAGA AAAGATACCT GAAAAGAACC ACACGAGACT GCTACAAATC CCCACATCAG 
AATAACATTC AGAAGAAGAG AGATTACAGA TCATTTTCAG ACAGAGAAAG TACCTTTGCA 
AACAATGAAT GCAGGCACGG TGTTCAAGAA CAACTGTCTT CCAGCGCTGT CGTGAATGCA 
GAAAACCAGG CCAAACATAA GTGTGATGCT GTGCTAAGTG ATGAAGTCCA AGTTAGCTTG 
TCTTTGTTAG GAAACCATGT CTTACTTCCT TCTCAGCCTG TGCTACAGGT GAAAAATTCA 
AGAGACAAAT ACTGTATAAC AGATTTTGCA GAAGAATTGG CAGAAACAGT GGTCTCTATG 
GCAACAGAAA TAGCTGCCAT TTGTCTTGAA AACTCAAATG GAAAGCAGCC CTGGTTCTGT 
GCATGGAAGA GAGGCAATGA ATATCTGGTG ACCCAGAGTA TATCATGCAG AACTATGAAA 
AGGAAAAAGG AAACCCATGC TAATGGCTCA GTTGTTCGGA AACACAGGGC ACCTCGACTG 
AGTGAGATCA AAAGAAAGAC AGATGAGCAT CCTGAGCTAA AGGAAAGGTT GATGAATCGA 
GTAGTAGATG AATCTATAAA CCTTGAGGAC ACACCAGATT CTGTCAATAT ATTTGCAAAT 
GAAGTGGCTG CAAAGATCAT GAACCTCACT GAACTCTCCA TGGTTGACAG TATCTGGCAA 
GGTCCAAACC ATCCCAGGAA CAGGCTGCAC TGTGAAAGAT GGAGCCGAGC CAAGGCCTCA 
AGCTGTGAGA GCATACCAGA GGAGGACATG GATTCCAAAG CCTCTGCCAA TACTCTAGGC 
CTAATGAATG CCCTTGGTCA GCCTGTGAGC CAGACAAGTT CCGTCTCAAA GCAGTCTAGT 
TGTGAAAGCA TTACAGATGA GTTTTCAAGA TTTATGGTGA ACCAAATGGA AAATGAGGGA 
AGAGGCTTTG ATTTATTACT AGATTATTAT GCAGGAAAAA ATGCAAACAA CATCTTAACG 
TCGGCTTTGC AACAGGTTGC TAAGAAAAAT GGTCATCTTA ATGTACGGCC AAGTTGCCCA 
TCCAAGCAGT CCAGCACGGA AAGCATAACA GAAGAATTTT ACAGGTATAT GCTAAAAGAA 
ATAGAAAAGG AAAATAAAGA TAATGCATCT TCCCCTCGGA ATTCAAAGGA CTGGTGTGGC 
AGTTTGCTAC CACCCTCTCT GCGATCACCT TTTTGCTTTA GACAGTCCTC AGTGCCTGAT 
GGCAGATCAT CAGGTTCTAG ACTAACAGTT AACATCCCAG TCAAGGCAAA TTCATTAGAT 
GGATTTGCCC AACATCACCA GGATTCCTTA AGTGTACAGC CAGTCAGTAC TGTGGCTTCT 
TCAGGTCTTT GCAAGTCTGA CTCATGCCTG TACCAAAGAT GCAAGACTGA CCAGATAACA 
GATATGTTGA TTCACGAGAC GTGGGCAAGC TCTATTGAAT CTCTAATGCG CAAAAACAAA 
ATCATAACAG ATGAGGCAGA GGTTGCAGAG GTAGATCATT TTCACAGTGA TTCCCCACCA 
CATGTGCAAC AGTATGCAAA CAGACTGGCT GCAAATATTG TTGAAAGTGG TAAAAATTTA 
ATTGTTGTCC AGCAGGATGC CTTTGATTCT GCAAGCCAAG AACACATGCT GGAAAGCAAA 
CATCCCCAGA GTGCAACTCA GATTCAAGTA GAACCCAAAA GGAGTGGAAT AAATTTGGAT 
GAGCATATGA AGAACCCTGA ATGCCTCCCT GCAGGTCAGC ACAGGGAAGT GCCTTTAATT 
CAGATAGAAA CTGATCAACG AGACGAGTCA GATAAAGACT CTGAATCCTT AACTTTACAT 
GAGCTTTCTG AAAAGGAGCA TCAAGGCAAG GAAAAGCCTC CAGAAGCTTA TGCTGGGAAG 
CACATGGTTT CCAGTACCCT ACTGAATAGC AAAAGCAGTC AGAGCAAACC AGATGCTGAA 
ATAACGGGAG AGACAAAAGC AGCTGAAGAA TTTCCAACAC ACCTCAGCAG CAGTGAAGAA 
AGCACTGGCA GCTGGTCCCA GCTAGCCAAT GATGAAGACA ATCCTGATGA CACAAGTAGC 
TACTTACAAC TCAGCGAACG ATCTCTGAGT GACTTAGTAG AAGAAAAGGT TTTCCTTAAA 
GAACAGACAG ATAATACAGA GGAAAGTACT TCTGGGCTCT CAGTGGGAAC AGCCAATTAT 
CAGAAAGAAC TCCTGGTGAT TAATTTTGAT CTGGAACCAG AATGCCCCAA CGCAGAGCTG 
CGAGCCACCC TACAGTGGAT AGCTGCTTCT GAACTTGGAA TCCCAACCAT CTATTTTAAA 
AAATCTCAGG AAAACAGAAT TGAAAAGTTT TTAGATGTTG TGCAGCTAGT TCAACGGAAG 
TCCTGGAAAG TGGGTGACAT CTTTCATGCT GTGGTGCAGT ACTGCAAACT CAGTGAGGAG 
CGCAAAGAGA AGACACCAAG CCTGTTCGAT TGGCTCCTTG AACTGGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPHKAP
Entrez Gene ID	424789
Full Name	SPHK1 interactor, AKAP domain containing
Synonyms	4930544G21RIK
General protein information	Preferred Names SPHK1 interactor, AKAP domain containing Names A-kinase anchor protein SPHKAP sphingosine kinase type 1-interacting protein
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Canis lupus familiaris (dog)	SPHKAP	XP_005635852.1
Gallus gallus (chicken)	SPHKAP	NP_001171003.1
Xenopus tropicalis (tropical clawed frog)	sphkap	XP_002933413.2
Bos taurus (cattle)	SPHKAP	XP_002685689.2
Mus musculus (house mouse)	Sphkap	NP_766018.3
Danio rerio (zebrafish)	sphkap	NP_001076514.1
Homo sapiens (human)	SPHKAP	NP_001136116.1
Pan troglodytes (chimpanzee)	SPHKAP	XP_001137841.1
	SPHKAP	XP_001111587.1
Rattus norvegicus (Norway rat)	Sphkap	NP_001120964.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPHKAP cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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