Sphkap cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sphkap
Entrez Gene ID	316561
Full Name	SPHK1 interactor, AKAP domain containing
Synonyms	RGD1311951,Skip
General protein information	Preferred Names SPHK1 interactor, AKAP domain containing Names A-kinase anchor protein SPHKAP SPHK1-interactor and AKAP domain-containing protein sphingosine kinase type 1-interacting protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q34

mRNA and Protein(s)

mRNA	Protein	Name
XM_006245231.3	XP_006245293.1	A-kinase anchor protein SPHKAP isoform X2
XM_008767251.2	XP_008765473.1	A-kinase anchor protein SPHKAP isoform X4
XM_006245230.3	XP_006245292.1	A-kinase anchor protein SPHKAP isoform X1
XM_017596483.1	XP_017451972.1	A-kinase anchor protein SPHKAP isoform X3
XM_017596484.1	XP_017451973.1	A-kinase anchor protein SPHKAP isoform X5
XM_006245233.3	XP_006245295.1	A-kinase anchor protein SPHKAP isoform X6
NM_001127492.1	NP_001120964.1	A-kinase anchor protein SPHKAP
XM_006245234.3	XP_006245296.1	A-kinase anchor protein SPHKAP isoform X7

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPHKAP	XP_005635852.1
Gallus gallus (chicken)	SPHKAP	NP_001171003.1
Xenopus tropicalis (tropical clawed frog)	sphkap	XP_002933413.2
Bos taurus (cattle)	SPHKAP	XP_002685689.2
Mus musculus (house mouse)	Sphkap	NP_766018.3
Danio rerio (zebrafish)	sphkap	NP_001076514.1
Homo sapiens (human)	SPHKAP	NP_001136116.1
Pan troglodytes (chimpanzee)	SPHKAP	XP_001137841.1
	SPHKAP	XP_001111587.1
Rattus norvegicus (Norway rat)	Sphkap	NP_001120964.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of insulin signaling in skeletal muscle by PIP3 phosphatase, SKIP, and endoplasmic reticulum molecular chaperone glucose-regulated protein 78.
Ijuin T, Hatano N, Hosooka T, Takenawa T
Biochimica et biophysica acta1853(12)3192-201(2015 Dec)

Up-regulation of SKIP relates to retinal ganglion cells apoptosis after optic nerve crush in vivo.
Wu Y, Xu F, Huang H, Chen L, Wen M, Jiang L, Lu L, Li L, Song D, Zeng S, Li L, Li M
Journal of molecular histology45(6)715-21(2014 Dec)

An entirely specific type I A-kinase anchoring protein that can sequester two molecules of protein kinase A at mitochondria.
Means CK, Lygren B, Langeberg LK, Jain A, Dixon RE, Vega AL, Gold MG, Petrosyan S, Taylor SS, Murphy AN, Ha T, Santana LF, Tasken K, Scott JD
Proceedings of the National Academy of Sciences of the United States of America108(48)E1227-35(2011 Nov)

Sphingosine kinase interacting protein is an A-kinase anchoring protein specific for type I cAMP-dependent protein kinase.
Kovanich D, van der Heyden MA, Aye TT, van Veen TA, Heck AJ, Scholten A
Chembiochem : a European journal of chemical biology11(7)963-71(2010 May)

Analysis of the cGMP/cAMP interactome using a chemical proteomics approach in mammalian heart tissue validates sphingosine kinase type 1-interacting protein as a genuine and highly abundant AKAP.
Scholten A, Poh MK, van Veen TA, van Breukelen B, Vos MA, Heck AJ
Journal of proteome research5(6)1435-47(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings underscore the specific and prominent role of SKIP and GRP78 in the regulation of insulin-dependent PI 3-kinase signaling in skeletal muscle
Title: Regulation of insulin signaling in skeletal muscle by PIP3 phosphatase, SKIP, and endoplasmic reticulum molecular chaperone glucose-regulated protein 78.

Up-regulation of SKIP in the retina is associated with retinal ganglion cells death after optic nerve crush.
Title: Up-regulation of SKIP relates to retinal ganglion cells apoptosis after optic nerve crush in vivo.

The following Sphkap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sphkap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa62776	XM_017596483.1 Latest version!	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa62777	XM_017596484.1 Latest version!	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa01680	NM_001127492.1 Latest version!	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa32395	XM_006245230.3 Latest version!	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa32396	XM_006245231.3 Latest version!	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa32398	XM_006245233.3 Latest version!	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa32399	XM_006245234.3 Latest version!	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa47550	XM_008767251.2 Latest version!	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sphkap ( XM_017596483.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451972.1 Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa62776
Clone ID Related Accession (Same CDS sequence)	XM_017596483.1
Accession Version	XM_017596483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein SPHKAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTAG TTGAACCAAA CTCTGCTACC TGCCTTTCTG CTGCCCCGCC GGTCAAGAGA 
CACTGCCAAG AATCCAGCAA CGTGGAATCA CCACTGATGC CCGAGGACTC GGAGCCACAG 
CAGATCACCA GCAGTGCAGC AGGCAGCCTG GCTGGCTCCA TCACGGCCTG TAAGAAGGTT 
CTTCGAAGCA ATAGCTTGCT GGAGTCCACA GACTACTGGT TGCAGAACCA GAGGACGCCT 
TGCCAGATTG GGTTTGTGGA AGACGAGTCT GAAAACTGTG CTTCTGTCTG CTTTGTGAAC 
CTGGATGTCA ACAAGGATGG CTGCAGCACA GAGAACCTGC AGCAGAAACT GGTCAACGTT 
TCACCAGACC TTCCAAAACT CATCAACTCC ATGAATGTCC AGAAGCCAAA AGAAAATGAA 
ATTGTCCTCC TAAGTGGGCT AGCCTCTGGA AACCGCCAGG CAGATTTTGA TGTTTCACAG 
TGCCCTTGGC TACCAGATAT CTGCTTGGTC CAGTGCGCAA GAGGGAACAG ACCAAACAGT 
ACCAACTGCA TCATCTTTGA GATCAACAAG TTCTTAATTG GTCTGGAAGT GGTGCAGGAG 
CGACAGCTCC ACCTGGAGAC CAGCGTGTTG AAGCTGGAGG ATGATACAAA CTGTTCCCTG 
TCTTCGATTG AAGAAGATTT CCTTACAGCC TCAGAGCATT TGGAGGAAGA AATCGAGGTG 
GAAGACTGCA AAAGTGGTTT GGAAACTATA AATGTGTCAG CTAACGTTCT GGAAAGCAAA 
AGGCCAAAGG AAGCTACCCA GGAAGGATGG GATTACCACA AGGAAATATT GCATTGTGCT 
TTAGGTGAAA AACACATCAG AAAACACCGT ATGCCCTCAA TGAAAACAGA AAGGTCAAAG 
GAAAACACAG CAGAGAACAC AGCCCTGCAA AGTCTCAATC CTTCAGCCAG ACCATCACGC 
TTGAAAAGTG AAGTGGCGGG GAGCAAGCAG CCAGCCACGA ACTACTCTTA CCCCGAAAAT 
ATCAGAGGAG AAATAGAAAC ATCTCAGATG CTGTTCATCC CCAGGGATGC CTATCTCTCC 
ATGGTTTCTT CATGTGGTGT ACTCACTGAG CAGGGAAGCA ACCACAGAGA CCATGACGCC 
ACTCCAAACT CCCTTCCTCC CGTTCAAAAT GGAGAAGCCA CCGCGGGCGA GTACGCTACC 
AACTTAGCAG AATCTGTAAT GCAAGATGCC TTCATCCGAT TGTCTCAGTC GCAACCTACA 
CTCCCCCAGG AATCTGCAGT CAGTTTCTCT GTGGGAAGCG CTCTGCTCCC CAGTGGTTGT 
TGCACCAAAG ATATGGTGGT CCCTCGTTCA TGGAATGAGC TTCCTAAAAT TGTCATCGTG 
CAGAGCCCAG AGGGCAGCGA CACAGCCCCT GAGCCAAGTG TCTCCTCCTG GCCCGACCTG 
GAAGTGTCTG TCGAGACCCC GGGCATCTTT TCTGAAGAGA GCTCCAGCAG ACCCACTCAG 
AGTGCCCTAG AAGTTGCTTT GGCTTGTGCA GCCACTGTGA TTGGAACCAC TTCCAGTCCA 
CAGGCCACAG AAAGATTCAC CATGGAGCAA GAATCTCTGG TATCTACCTA CACCCAGAGA 
GGCAATGGGA TACAGCAAAC TCCAGTGCCC AGAGTATTCA TGGGACCTTC TACAACTGAA 
TACTCCTTCC CATCAGCTCT GTGTGGCATG ACCCAAGTGG CAAGCGCTGT TGCTGTCTGT 
GGCCTAGGGG AAAGAGAAGA GGTGACATAT TCTGTAGCTC CAAGTGACCT CCTGCCCACA 
CCTGGGGCTT CTGAAGAAAG ATCCTCCATT GGAAGTTTAG TGACAGAGGA AAGCACAGAA 
CTGGGGAAGG AAGCCATTGC AGAGGCTCTG CTCAGGGAGG CTACTCTGGT TTTAGCGAGG 
CCGAATGCAT ACAGCAGCGT CGGAGAGCTC TTGGAATCTG TGAACCGGAG GATCATAGAA 
ACCACTTCTA AAACCCAGAT GCTGTGCACA GAAAATGTTC GAAGGAATGA ACTGGCACAC 
ACCTTGTCCA ATGTCATCCT CAAGCATTCC ATTGATGAGC TGCACCAGAA AAATATCATG 
GCTCACCCCA CCGAGGAAAG GCATCCCGGT GGGACTCTGA ACACCTTAAT GGAAAGTGTG 
AACCAGTTGC TGCACAACGT GATGTGCTTC ACCTTCAGAA AGATGAATCA CATCGTAACA 
CTGGGTGAGC ATCCCGCTGG CCTTTCTAAG GAGACTGTCA CTTGGACAGA GTCAGGGCCC 
TTAGGTTGCC AGTCATTTGA TAAGGCTGCT GGTCATGCAT GGGTTAAAGC CTCGGCTTGC 
TCCAGCAGCC ATCCCTCTAG CAATGCACAC GGTACTGGCC TTGTCATCAG AGATCTTGTA 
GAAGATGCAT CTCCTAAGCC AGACAAGGGA GGAGCAAGGC CAGAACTGGT CAACAACCCC 
AGGCTGCAAT CTGAATTCTC ATGCAGTCAC AGAATGCTTG ATTCAACTGC TAGGACATTC 
CCCAAGGAAA TGTATCCAAA AGGGATTGTG GGAGAGGATA CAAGAAACCC TCTTCATACA 
CTAAGTTATG ACAGCAGTGA ACAAAGAACT TCTACAGATA TAGGAAAATT AACAACAGTA 
GGAGAGGTCC GTAGTACCTT CCAGGAATCT GAAGACAGCA TTGTTCCAAA AGCCCAAGAG 
AAACACACTT GTGCCACAAC TCTAAACAAC GAAGCTCAAA TTAACCTATC CTTGTTAGGG 
GATGACCTAG TGGTTCCTGA TCAATCTACA CTAGAGGCAA AGCAGTCCGA GGTCTATGGC 
ATCACAAACT TTGCGGAAGA ATTGGCAGAG ACTGTTGTCT CCATGGCAAC TGAAATCGCA 
GCAATCTGCC TTGACAACTC CCATGGAAAA CAGCCGTGGT TTTGTGCTTG GAAGAGAGGG 
AACGAATTTC TGATGACGCC TAACGCATCC TGCCGATCCT TGAAGAGGAA GAAGGAAAAC 
TCGGGCACGG GAAGCACTGT GAGGAAACAC AAGCCACCTC GCCTCAGTGA GATCAAGAGA 
AAAGCAGATG AGCACCCAGA GCTGAAAGAG AAGCTGATGA ACAGAGTCAT GGATGAGTCC 
ATGAACCTCG AAGACATTCC CGATTCCGTC AGTACTTTTG CAAATGAAGT GGCAGCCAAG 
ATCATGAACC TAACAGAATT CTCCACGGTG GATGGAGTGT GGCAAACCCA GAGTTGTTCC 
CGGAATCGGC TTCTGGGTGG TGATAGGTGG AACCGGCTGA AGGCCTCGAG CTGTGAAAGT 
ATTCCGGAGG AGGACTCTGA AGCCAGGGTC TTTGTAAACA GCCTGGGTTT AATGAGTACC 
TTAAGCCAAC CCGTTAGCAG GGCCAGCTCA GTCTCCAAAC AGTCCAGCTG TGAGAGCATC 
ACCGATGAGT TTTCCAGGTT CATGGTGAAA CAGATGGAAA ACGAAGGGAG GGGGTTCGAG 
TTGCTGCTGG ATTACTACGC TGGCAAAAAT GCCAGCAGTA TCCTGAACTC AGCAATCCAG 
CAGGCATGCC AGAAAAACGA CCACCTCAAC GTAAGACCAA GCTGCCCCTC TAAGCAGTCC 
AGCACAGAAA GCATAACAGA GGAGTTCTAT AAGTACATGC TGAGAGACAT TGCCAAAGAA 
AACAAAGATG GCGCCCTGTC CAGACGAAGC AGCCATGATT GGAGCACGGG CTTGCTGTCT 
CCTTCCGCGC GATCCCCACT GTGTTACAGG CAGTCATCTA TGCCTGACAG CAGGTCCCCG 
TGCTCCAGGT TAACAGTAAA TGCACCTATC AAAGCCAACT CCTTAGATGG TTTTGCCCAA 
AACTGCCCTC AAGATTCTAT AAATGTACAG CCAGTCAGTA GGGCTTCCTC GTCTGGCCTC 
TGCAAATCAG ACTCCTGTTT ATATCGCCGA AGTGGGACTG ACCAGATCAC AAACATGCTA 
ATTCATGAAA CATGGGCCAG CTCCATTGAG GCTCTCATGA GAAAGAACAA AATCATTGCT 
GATGACGGCG AGGCAGCTAA CGCTAGCCCT GGTCCTGTTT CTGGCGGCTC GCCTTCGCAA 
GTAGAGAAGT GTGCCAACAG ACTAGTCACC GGCACAGGAC ACAAGGGACC AGCTCTACTT 
GTACAGGAGT CTGTTGATTA CCAGAGAAAG GATGCTGTCA CTGAAGGCAA TTGTTCCCCA 
GTGTCATCTC CAAGCAAAAT GGCTCCTGTT AAAAAGCCCA GTGGTTTTGA TCCTACAAGA 
GAAACCTCTG CATGCCACAA TGCTGTTGCT CTCAAAAGCC CCAGGCGGTC TCTTTGCTCA 
AGGGAGGTGC CTTTGATTCA GATAGAGACC GATCAGAAAG AAGAATGCGT TGGAGAATCT 
GAAACCTTAC TTCCCCAAAG TGGCTCCCTA GAGGAAGCAG AAGGGCCCCA GCCTGAGGAA 
ACCATCCCAG ATGTGGCCAG AAGCGAAGAC ACAGCCCTGA GCGCCTGTCA AAGCTCTCAT 
GACAGCCTTG AGACCAGAGA AGAATTAGAA GTGGATGTCT TGAAAGAAGA CATAACCCTA 
GATGAGTCCC GGAACCCTCC TAGCAGCAGC GAAGAGAGTA CAGGCAGCTG GTCCCAGCTG 
GCCAATGAGG AGGACAACCC AGATGACACA AGTAGCTTTC TGCAGCTCAG TGAGCGGTCC 
ATGAGCAATG GCAACACTAG TGGCACTAGC AGTCTTGGCA TTATGGACCT GGACATTTAT 
CAGGAAAGCA TACCGTCTTC TCCCATGATT AATGAATTAG TAGAAGAAAA GGAGATTCTT 
AAAGAACAAT CAGAAAGCGT AAAGGAACGT GCCTCTGGAC TGCCAGGGAG AGCAGCCAGC 
CCCCAGAGGA GTCTACTGGT GATCAACTTT GACCTGGAGC CAGAGTGTCC TGATGCTGAG 
CTGCGAGCCA CTCTGCAGTG GATAGCTGCC TCCGAGCTGG GCATCCCAAC AATCTACTTT 
AAGAAATCTC AGGAAAGCAG AATTGAAAAG TTTCTAGATG TTGTGAAGCT TGTTCATCAG 
AAGTCCTGGA AGGTGGGAGA TATATTTCAT GCCGTTGTCC AGTACTGCAA AATGCATGCA 
GAGCAGAAAG AGGGAACTCC GAGTCTCTTT GACTGGCTCC TGGAACTAGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451972.1
CDS	423..5576
Translation	MWVVEPNSATCLSAAPPVKRHCQESSNVESPLMPEDSEPQQITSSAAGSLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDGCSTENLQQKLVNVSPDLPKLINSMNVQKPKENEIVLLSGLASGNRQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETSVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVEDCKSGLETINVSANVLESKRPKEATQEGWDYHKEILHCALGEKHIRKHRMPSMKTERSKENTAENTALQSLNPSARPSRLKSEVAGSKQPATNYSYPENIRGEIETSQMLFIPRDAYLSMVSSCGVLTEQGSNHRDHDATPNSLPPVQNGEATAGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSVGSALLPSGCCTKDMVVPRSWNELPKIVIVQSPEGSDTAPEPSVSSWPDLEVSVETPGIFSEESSSRPTQSALEVALACAATVIGTTSSPQATERFTMEQESLVSTYTQRGNGIQQTPVPRVFMGPSTTEYSFPSALCGMTQVASAVAVCGLGEREEVTYSVAPSDLLPTPGASEERSSIGSLVTEESTELGKEAIAEALLREATLVLARPNAYSSVGELLESVNRRIIETTSKTQMLCTENVRRNELAHTLSNVILKHSIDELHQKNIMAHPTEERHPGGTLNTLMESVNQLLHNVMCFTFRKMNHIVTLGEHPAGLSKETVTWTESGPLGCQSFDKAAGHAWVKASACSSSHPSSNAHGTGLVIRDLVEDASPKPDKGGARPELVNNPRLQSEFSCSHRMLDSTARTFPKEMYPKGIVGEDTRNPLHTLSYDSSEQRTSTDIGKLTTVGEVRSTFQESEDSIVPKAQEKHTCATTLNNEAQINLSLLGDDLVVPDQSTLEAKQSEVYGITNFAEELAETVVSMATEIAAICLDNSHGKQPWFCAWKRGNEFLMTPNASCRSLKRKKENSGTGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSTVDGVWQTQSCSRNRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSILNSAIQQACQKNDHLNVRPSCPSKQSSTESITEEFYKYMLRDIAKENKDGALSRRSSHDWSTGLLSPSARSPLCYRQSSMPDSRSPCSRLTVNAPIKANSLDGFAQNCPQDSINVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDGEAANASPGPVSGGSPSQVEKCANRLVTGTGHKGPALLVQESVDYQRKDAVTEGNCSPVSSPSKMAPVKKPSGFDPTRETSACHNAVALKSPRRSLCSREVPLIQIETDQKEECVGESETLLPQSGSLEEAEGPQPEETIPDVARSEDTALSACQSSHDSLETREELEVDVLKEDITLDESRNPPSSSEESTGSWSQLANEEDNPDDTSSFLQLSERSMSNGNTSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESVKERASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVHQKSWKVGDIFHAVVQYCKMHAEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_017596483.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596483.1

ORF Insert Sequence:

ATGTGGGTAG TTGAACCAAA CTCTGCTACC TGCCTTTCTG CTGCCCCGCC GGTCAAGAGA 
CACTGCCAAG AATCCAGCAA CGTGGAATCA CCACTGATGC CCGAGGACTC GGAGCCACAG 
CAGATCACCA GCAGTGCAGC AGGCAGCCTG GCTGGCTCCA TCACGGCCTG TAAGAAGGTT 
CTTCGAAGCA ATAGCTTGCT GGAGTCCACA GACTACTGGT TGCAGAACCA GAGGACGCCT 
TGCCAGATTG GGTTTGTGGA AGACGAGTCT GAAAACTGTG CTTCTGTCTG CTTTGTGAAC 
CTGGATGTCA ACAAGGATGG CTGCAGCACA GAGAACCTGC AGCAGAAACT GGTCAACGTT 
TCACCAGACC TTCCAAAACT CATCAACTCC ATGAATGTCC AGAAGCCAAA AGAAAATGAA 
ATTGTCCTCC TAAGTGGGCT AGCCTCTGGA AACCGCCAGG CAGATTTTGA TGTTTCACAG 
TGCCCTTGGC TACCAGATAT CTGCTTGGTC CAGTGCGCAA GAGGGAACAG ACCAAACAGT 
ACCAACTGCA TCATCTTTGA GATCAACAAG TTCTTAATTG GTCTGGAAGT GGTGCAGGAG 
CGACAGCTCC ACCTGGAGAC CAGCGTGTTG AAGCTGGAGG ATGATACAAA CTGTTCCCTG 
TCTTCGATTG AAGAAGATTT CCTTACAGCC TCAGAGCATT TGGAGGAAGA AATCGAGGTG 
GAAGACTGCA AAAGTGGTTT GGAAACTATA AATGTGTCAG CTAACGTTCT GGAAAGCAAA 
AGGCCAAAGG AAGCTACCCA GGAAGGATGG GATTACCACA AGGAAATATT GCATTGTGCT 
TTAGGTGAAA AACACATCAG AAAACACCGT ATGCCCTCAA TGAAAACAGA AAGGTCAAAG 
GAAAACACAG CAGAGAACAC AGCCCTGCAA AGTCTCAATC CTTCAGCCAG ACCATCACGC 
TTGAAAAGTG AAGTGGCGGG GAGCAAGCAG CCAGCCACGA ACTACTCTTA CCCCGAAAAT 
ATCAGAGGAG AAATAGAAAC ATCTCAGATG CTGTTCATCC CCAGGGATGC CTATCTCTCC 
ATGGTTTCTT CATGTGGTGT ACTCACTGAG CAGGGAAGCA ACCACAGAGA CCATGACGCC 
ACTCCAAACT CCCTTCCTCC CGTTCAAAAT GGAGAAGCCA CCGCGGGCGA GTACGCTACC 
AACTTAGCAG AATCTGTAAT GCAAGATGCC TTCATCCGAT TGTCTCAGTC GCAACCTACA 
CTCCCCCAGG AATCTGCAGT CAGTTTCTCT GTGGGAAGCG CTCTGCTCCC CAGTGGTTGT 
TGCACCAAAG ATATGGTGGT CCCTCGTTCA TGGAATGAGC TTCCTAAAAT TGTCATCGTG 
CAGAGCCCAG AGGGCAGCGA CACAGCCCCT GAGCCAAGTG TCTCCTCCTG GCCCGACCTG 
GAAGTGTCTG TCGAGACCCC GGGCATCTTT TCTGAAGAGA GCTCCAGCAG ACCCACTCAG 
AGTGCCCTAG AAGTTGCTTT GGCTTGTGCA GCCACTGTGA TTGGAACCAC TTCCAGTCCA 
CAGGCCACAG AAAGATTCAC CATGGAGCAA GAATCTCTGG TATCTACCTA CACCCAGAGA 
GGCAATGGGA TACAGCAAAC TCCAGTGCCC AGAGTATTCA TGGGACCTTC TACAACTGAA 
TACTCCTTCC CATCAGCTCT GTGTGGCATG ACCCAAGTGG CAAGCGCTGT TGCTGTCTGT 
GGCCTAGGGG AAAGAGAAGA GGTGACATAT TCTGTAGCTC CAAGTGACCT CCTGCCCACA 
CCTGGGGCTT CTGAAGAAAG ATCCTCCATT GGAAGTTTAG TGACAGAGGA AAGCACAGAA 
CTGGGGAAGG AAGCCATTGC AGAGGCTCTG CTCAGGGAGG CTACTCTGGT TTTAGCGAGG 
CCGAATGCAT ACAGCAGCGT CGGAGAGCTC TTGGAATCTG TGAACCGGAG GATCATAGAA 
ACCACTTCTA AAACCCAGAT GCTGTGCACA GAAAATGTTC GAAGGAATGA ACTGGCACAC 
ACCTTGTCCA ATGTCATCCT CAAGCATTCC ATTGATGAGC TGCACCAGAA AAATATCATG 
GCTCACCCCA CCGAGGAAAG GCATCCCGGT GGGACTCTGA ACACCTTAAT GGAAAGTGTG 
AACCAGTTGC TGCACAACGT GATGTGCTTC ACCTTCAGAA AGATGAATCA CATCGTAACA 
CTGGGTGAGC ATCCCGCTGG CCTTTCTAAG GAGACTGTCA CTTGGACAGA GTCAGGGCCC 
TTAGGTTGCC AGTCATTTGA TAAGGCTGCT GGTCATGCAT GGGTTAAAGC CTCGGCTTGC 
TCCAGCAGCC ATCCCTCTAG CAATGCACAC GGTACTGGCC TTGTCATCAG AGATCTTGTA 
GAAGATGCAT CTCCTAAGCC AGACAAGGGA GGAGCAAGGC CAGAACTGGT CAACAACCCC 
AGGCTGCAAT CTGAATTCTC ATGCAGTCAC AGAATGCTTG ATTCAACTGC TAGGACATTC 
CCCAAGGAAA TGTATCCAAA AGGGATTGTG GGAGAGGATA CAAGAAACCC TCTTCATACA 
CTAAGTTATG ACAGCAGTGA ACAAAGAACT TCTACAGATA TAGGAAAATT AACAACAGTA 
GGAGAGGTCC GTAGTACCTT CCAGGAATCT GAAGACAGCA TTGTTCCAAA AGCCCAAGAG 
AAACACACTT GTGCCACAAC TCTAAACAAC GAAGCTCAAA TTAACCTATC CTTGTTAGGG 
GATGACCTAG TGGTTCCTGA TCAATCTACA CTAGAGGCAA AGCAGTCCGA GGTCTATGGC 
ATCACAAACT TTGCGGAAGA ATTGGCAGAG ACTGTTGTCT CCATGGCAAC TGAAATCGCA 
GCAATCTGCC TTGACAACTC CCATGGAAAA CAGCCGTGGT TTTGTGCTTG GAAGAGAGGG 
AACGAATTTC TGATGACGCC TAACGCATCC TGCCGATCCT TGAAGAGGAA GAAGGAAAAC 
TCGGGCACGG GAAGCACTGT GAGGAAACAC AAGCCACCTC GCCTCAGTGA GATCAAGAGA 
AAAGCAGATG AGCACCCAGA GCTGAAAGAG AAGCTGATGA ACAGAGTCAT GGATGAGTCC 
ATGAACCTCG AAGACATTCC CGATTCCGTC AGTACTTTTG CAAATGAAGT GGCAGCCAAG 
ATCATGAACC TAACAGAATT CTCCACGGTG GATGGAGTGT GGCAAACCCA GAGTTGTTCC 
CGGAATCGGC TTCTGGGTGG TGATAGGTGG AACCGGCTGA AGGCCTCGAG CTGTGAAAGT 
ATTCCGGAGG AGGACTCTGA AGCCAGGGTC TTTGTAAACA GCCTGGGTTT AATGAGTACC 
TTAAGCCAAC CCGTTAGCAG GGCCAGCTCA GTCTCCAAAC AGTCCAGCTG TGAGAGCATC 
ACCGATGAGT TTTCCAGGTT CATGGTGAAA CAGATGGAAA ACGAAGGGAG GGGGTTCGAG 
TTGCTGCTGG ATTACTACGC TGGCAAAAAT GCCAGCAGTA TCCTGAACTC AGCAATCCAG 
CAGGCATGCC AGAAAAACGA CCACCTCAAC GTAAGACCAA GCTGCCCCTC TAAGCAGTCC 
AGCACAGAAA GCATAACAGA GGAGTTCTAT AAGTACATGC TGAGAGACAT TGCCAAAGAA 
AACAAAGATG GCGCCCTGTC CAGACGAAGC AGCCATGATT GGAGCACGGG CTTGCTGTCT 
CCTTCCGCGC GATCCCCACT GTGTTACAGG CAGTCATCTA TGCCTGACAG CAGGTCCCCG 
TGCTCCAGGT TAACAGTAAA TGCACCTATC AAAGCCAACT CCTTAGATGG TTTTGCCCAA 
AACTGCCCTC AAGATTCTAT AAATGTACAG CCAGTCAGTA GGGCTTCCTC GTCTGGCCTC 
TGCAAATCAG ACTCCTGTTT ATATCGCCGA AGTGGGACTG ACCAGATCAC AAACATGCTA 
ATTCATGAAA CATGGGCCAG CTCCATTGAG GCTCTCATGA GAAAGAACAA AATCATTGCT 
GATGACGGCG AGGCAGCTAA CGCTAGCCCT GGTCCTGTTT CTGGCGGCTC GCCTTCGCAA 
GTAGAGAAGT GTGCCAACAG ACTAGTCACC GGCACAGGAC ACAAGGGACC AGCTCTACTT 
GTACAGGAGT CTGTTGATTA CCAGAGAAAG GATGCTGTCA CTGAAGGCAA TTGTTCCCCA 
GTGTCATCTC CAAGCAAAAT GGCTCCTGTT AAAAAGCCCA GTGGTTTTGA TCCTACAAGA 
GAAACCTCTG CATGCCACAA TGCTGTTGCT CTCAAAAGCC CCAGGCGGTC TCTTTGCTCA 
AGGGAGGTGC CTTTGATTCA GATAGAGACC GATCAGAAAG AAGAATGCGT TGGAGAATCT 
GAAACCTTAC TTCCCCAAAG TGGCTCCCTA GAGGAAGCAG AAGGGCCCCA GCCTGAGGAA 
ACCATCCCAG ATGTGGCCAG AAGCGAAGAC ACAGCCCTGA GCGCCTGTCA AAGCTCTCAT 
GACAGCCTTG AGACCAGAGA AGAATTAGAA GTGGATGTCT TGAAAGAAGA CATAACCCTA 
GATGAGTCCC GGAACCCTCC TAGCAGCAGC GAAGAGAGTA CAGGCAGCTG GTCCCAGCTG 
GCCAATGAGG AGGACAACCC AGATGACACA AGTAGCTTTC TGCAGCTCAG TGAGCGGTCC 
ATGAGCAATG GCAACACTAG TGGCACTAGC AGTCTTGGCA TTATGGACCT GGACATTTAT 
CAGGAAAGCA TACCGTCTTC TCCCATGATT AATGAATTAG TAGAAGAAAA GGAGATTCTT 
AAAGAACAAT CAGAAAGCGT AAAGGAACGT GCCTCTGGAC TGCCAGGGAG AGCAGCCAGC 
CCCCAGAGGA GTCTACTGGT GATCAACTTT GACCTGGAGC CAGAGTGTCC TGATGCTGAG 
CTGCGAGCCA CTCTGCAGTG GATAGCTGCC TCCGAGCTGG GCATCCCAAC AATCTACTTT 
AAGAAATCTC AGGAAAGCAG AATTGAAAAG TTTCTAGATG TTGTGAAGCT TGTTCATCAG 
AAGTCCTGGA AGGTGGGAGA TATATTTCAT GCCGTTGTCC AGTACTGCAA AATGCATGCA 
GAGCAGAAAG AGGGAACTCC GAGTCTCTTT GACTGGCTCC TGGAACTAGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( XM_017596484.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451973.1 Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X5, mRNA.

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CloneID	ORa62777
Clone ID Related Accession (Same CDS sequence)	XM_017596484.1
Accession Version	XM_017596484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein SPHKAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTCA GGATGCTCCT GGGACACAGA GGACACAACG TGGAATCACC ACTGATGCCC 
GAGGACTCGG AGCCACAGCA GATCACCAGC AGTGCAGCAG GCAGCCTGGC TGGCTCCATC 
ACGGCCTGTA AGAAGGTTCT TCGAAGCAAT AGCTTGCTGG AGTCCACAGA CTACTGGTTG 
CAGAACCAGA GGACGCCTTG CCAGATTGGG TTTGTGGAAG ACGAGTCTGA AAACTGTGCT 
TCTGTCTGCT TTGTGAACCT GGATGTCAAC AAGGATGGCT GCAGCACAGA GAACCTGCAG 
CAGAAACTGG TCAACGTTTC ACCAGACCTT CCAAAACTCA TCAACTCCAT GAATGTCCAG 
AAGCCAAAAG AAAATGAAAT TGTCCTCCTA AGTGGGCTAG CCTCTGGAAA CCGCCAGGCA 
GATTTTGATG TTTCACAGTG CCCTTGGCTA CCAGATATCT GCTTGGTCCA GTGCGCAAGA 
GGGAACAGAC CAAACAGTAC CAACTGCATC ATCTTTGAGA TCAACAAGTT CTTAATTGGT 
CTGGAAGTGG TGCAGGAGCG ACAGCTCCAC CTGGAGACCA GCGTGTTGAA GCTGGAGGAT 
GATACAAACT GTTCCCTGTC TTCGATTGAA GAAGATTTCC TTACAGCCTC AGAGCATTTG 
GAGGAAGAAA TCGAGGTGGA AGACTGCAAA AGTGGTTTGG AAACTATAAA TGTGTCAGCT 
AACGTTCTGG AAAGCAAAAG GCCAAAGGAA GCTACCCAGG AAGGATGGGA TTACCACAAG 
GAAATATTGC ATTGTGCTTT AGGTGAAAAA CACATCAGAA AACACCGTAT GCCCTCAATG 
AAAACAGAAA GGTCAAAGGA AAACACAGCA GAGAACACAG CCCTGCAAAG TCTCAATCCT 
TCAGCCAGAC CATCACGCTT GAAAAGTGAA GTGGCGGGGA GCAAGCAGCC AGCCACGAAC 
TACTCTTACC CCGAAAATAT CAGAGGAGAA ATAGAAACAT CTCAGATGCT GTTCATCCCC 
AGGGATGCCT ATCTCTCCAT GGTTTCTTCA TGTGGTGTAC TCACTGAGCA GGGAAGCAAC 
CACAGAGACC ATGACGCCAC TCCAAACTCC CTTCCTCCCG TTCAAAATGG AGAAGCCACC 
GCGGGCGAGT ACGCTACCAA CTTAGCAGAA TCTGTAATGC AAGATGCCTT CATCCGATTG 
TCTCAGTCGC AACCTACACT CCCCCAGGAA TCTGCAGTCA GTTTCTCTGT GGGAAGCGCT 
CTGCTCCCCA GTGGTTGTTG CACCAAAGAT ATGGTGGTCC CTCGTTCATG GAATGAGCTT 
CCTAAAATTG TCATCGTGCA GAGCCCAGAG GGCAGCGACA CAGCCCCTGA GCCAAGTGTC 
TCCTCCTGGC CCGACCTGGA AGTGTCTGTC GAGACCCCGG GCATCTTTTC TGAAGAGAGC 
TCCAGCAGAC CCACTCAGAG TGCCCTAGAA GTTGCTTTGG CTTGTGCAGC CACTGTGATT 
GGAACCACTT CCAGTCCACA GGCCACAGAA AGATTCACCA TGGAGCAAGA ATCTCTGGTA 
TCTACCTACA CCCAGAGAGG CAATGGGATA CAGCAAACTC CAGTGCCCAG AGTATTCATG 
GGACCTTCTA CAACTGAATA CTCCTTCCCA TCAGCTCTGT GTGGCATGAC CCAAGTGGCA 
AGCGCTGTTG CTGTCTGTGG CCTAGGGGAA AGAGAAGAGG TGACATATTC TGTAGCTCCA 
AGTGACCTCC TGCCCACACC TGGGGCTTCT GAAGAAAGAT CCTCCATTGG AAGTTTAGTG 
ACAGAGGAAA GCACAGAACT GGGGAAGGAA GCCATTGCAG AGGCTCTGCT CAGGGAGGCT 
ACTCTGGTTT TAGCGAGGCC GAATGCATAC AGCAGCGTCG GAGAGCTCTT GGAATCTGTG 
AACCGGAGGA TCATAGAAAC CACTTCTAAA ACCCAGATGC TGTGCACAGA AAATGTTCGA 
AGGAATGAAC TGGCACACAC CTTGTCCAAT GTCATCCTCA AGCATTCCAT TGATGAGCTG 
CACCAGAAAA ATATCATGGC TCACCCCACC GAGGAAAGGC ATCCCGGTGG GACTCTGAAC 
ACCTTAATGG AAAGTGTGAA CCAGTTGCTG CACAACGTGA TGTGCTTCAC CTTCAGAAAG 
ATGAATCACA TCGTAACACT GGGTGAGCAT CCCGCTGGCC TTTCTAAGGA GACTGTCACT 
TGGACAGAGT CAGGGCCCTT AGGTTGCCAG TCATTTGATA AGGCTGCTGG TCATGCATGG 
GTTAAAGCCT CGGCTTGCTC CAGCAGCCAT CCCTCTAGCA ATGCACACGG TACTGGCCTT 
GTCATCAGAG ATCTTGTAGA AGATGCATCT CCTAAGCCAG ACAAGGGAGG AGCAAGGCCA 
GAACTGGTCA ACAACCCCAG GCTGCAATCT GAATTCTCAT GCAGTCACAG AATGCTTGAT 
TCAACTGCTA GGACATTCCC CAAGGAAATG TATCCAAAAG GGATTGTGGG AGAGGATACA 
AGAAACCCTC TTCATACACT AAGTTATGAC AGCAGTGAAC AAAGAACTTC TACAGATATA 
GGAAAATTAA CAACAGTAGG AGAGGTCCGT AGTACCTTCC AGGAATCTGA AGACAGCATT 
GTTCCAAAAG CCCAAGAGAA ACACACTTGT GCCACAACTC TAAACAACGA AGCTCAAATT 
AACCTATCCT TGTTAGGGGA TGACCTAGTG GTTCCTGATC AATCTACACT AGAGGCAAAG 
CAGTCCGAGG TCTATGGCAT CACAAACTTT GCGGAAGAAT TGGCAGAGAC TGTTGTCTCC 
ATGGCAACTG AAATCGCAGC AATCTGCCTT GACAACTCCC ATGGAAAACA GCCGTGGTTT 
TGTGCTTGGA AGAGAGGGAA CGAATTTCTG ATGACGCCTA ACGCATCCTG CCGATCCTTG 
AAGAGGAAGA AGGAAAACTC GGGCACGGGA AGCACTGTGA GGAAACACAA GCCACCTCGC 
CTCAGTGAGA TCAAGAGAAA AGCAGATGAG CACCCAGAGC TGAAAGAGAA GCTGATGAAC 
AGAGTCATGG ATGAGTCCAT GAACCTCGAA GACATTCCCG ATTCCGTCAG TACTTTTGCA 
AATGAAGTGG CAGCCAAGAT CATGAACCTA ACAGAATTCT CCACGGTGGA TGGAGTGTGG 
CAAACCCAGA GTTGTTCCCG GAATCGGCTT CTGGGTGGTG ATAGGTGGAA CCGGCTGAAG 
GCCTCGAGCT GTGAAAGTAT TCCGGAGGAG GACTCTGAAG CCAGGGTCTT TGTAAACAGC 
CTGGGTTTAA TGAGTACCTT AAGCCAACCC GTTAGCAGGG CCAGCTCAGT CTCCAAACAG 
TCCAGCTGTG AGAGCATCAC CGATGAGTTT TCCAGGTTCA TGGTGAAACA GATGGAAAAC 
GAAGGGAGGG GGTTCGAGTT GCTGCTGGAT TACTACGCTG GCAAAAATGC CAGCAGTATC 
CTGAACTCAG CAATCCAGCA GGCATGCCAG AAAAACGACC ACCTCAACGT AAGACCAAGC 
TGCCCCTCTA AGCAGTCCAG CACAGAAAGC ATAACAGAGG AGTTCTATAA GTACATGCTG 
AGAGACATTG CCAAAGAAAA CAAAGATGGC GCCCTGTCCA GACGAAGCAG CCATGATTGG 
AGCACGGGCT TGCTGTCTCC TTCCGCGCGA TCCCCACTGT GTTACAGGCA GTCATCTATG 
CCTGACAGCA GGTCCCCGTG CTCCAGGTTA ACAGTAAATG CACCTATCAA AGCCAACTCC 
TTAGATGGTT TTGCCCAAAA CTGCCCTCAA GATTCTATAA ATGTACAGCC AGTCAGTAGG 
GCTTCCTCGT CTGGCCTCTG CAAATCAGAC TCCTGTTTAT ATCGCCGAAG TGGGACTGAC 
CAGATCACAA ACATGCTAAT TCATGAAACA TGGGCCAGCT CCATTGAGGC TCTCATGAGA 
AAGAACAAAA TCATTGCTGA TGACGGCGAG GCAGCTAACG CTAGCCCTGG TCCTGTTTCT 
GGCGGCTCGC CTTCGCAAGT AGAGAAGTGT GCCAACAGAC TAGTCACCGG CACAGGACAC 
AAGGGACCAG CTCTACTTGT ACAGGAGTCT GTTGATTACC AGAGAAAGGA TGCTGTCACT 
GAAGGCAATT GTTCCCCAGT GTCATCTCCA AGCAAAATGG CTCCTGTTAA AAAGCCCAGT 
GGTTTTGATC CTACAAGAGA AACCTCTGCA TGCCACAATG CTGTTGCTCT CAAAAGCCCC 
AGGCGGTCTC TTTGCTCAAG GGAGGTGCCT TTGATTCAGA TAGAGACCGA TCAGAAAGAA 
GAATGCGTTG GAGAATCTGA AACCTTACTT CCCCAAAGTG GCTCCCTAGA GGAAGCAGAA 
GGGCCCCAGC CTGAGGAAAC CATCCCAGAT GTGGCCAGAA GCGAAGACAC AGCCCTGAGC 
GCCTGTCAAA GCTCTCATGA CAGCCTTGAG ACCAGAGAAG AATTAGAAGT GGATGTCTTG 
AAAGAAGACA TAACCCTAGA TGAGTCCCGG AACCCTCCTA GCAGCAGCGA AGAGAGTACA 
GGCAGCTGGT CCCAGCTGGC CAATGAGGAG GACAACCCAG ATGACACAAG TAGCTTTCTG 
CAGCTCAGTG AGCGGTCCAT GAGCAATGGC AACACTAGTG GCACTAGCAG TCTTGGCATT 
ATGGACCTGG ACATTTATCA GGAAAGCATA CCGTCTTCTC CCATGATTAA TGAATTAGTA 
GAAGAAAAGG AGATTCTTAA AGAACAATCA GAAAGCGTAA AGGAACGTGC CTCTGGACTG 
CCAGGGAGAG CAGCCAGCCC CCAGAGGAGT CTACTGGTGA TCAACTTTGA CCTGGAGCCA 
GAGTGTCCTG ATGCTGAGCT GCGAGCCACT CTGCAGTGGA TAGCTGCCTC CGAGCTGGGC 
ATCCCAACAA TCTACTTTAA GAAATCTCAG GAAAGCAGAA TTGAAAAGTT TCTAGATGTT 
GTGAAGCTTG TTCATCAGAA GTCCTGGAAG GTGGGAGATA TATTTCATGC CGTTGTCCAG 
TACTGCAAAA TGCATGCAGA GCAGAAAGAG GGAACTCCGA GTCTCTTTGA CTGGCTCCTG 
GAACTAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451973.1
CDS	1536..6647
Translation	MFLRMLLGHRGHNVESPLMPEDSEPQQITSSAAGSLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDGCSTENLQQKLVNVSPDLPKLINSMNVQKPKENEIVLLSGLASGNRQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETSVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVEDCKSGLETINVSANVLESKRPKEATQEGWDYHKEILHCALGEKHIRKHRMPSMKTERSKENTAENTALQSLNPSARPSRLKSEVAGSKQPATNYSYPENIRGEIETSQMLFIPRDAYLSMVSSCGVLTEQGSNHRDHDATPNSLPPVQNGEATAGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSVGSALLPSGCCTKDMVVPRSWNELPKIVIVQSPEGSDTAPEPSVSSWPDLEVSVETPGIFSEESSSRPTQSALEVALACAATVIGTTSSPQATERFTMEQESLVSTYTQRGNGIQQTPVPRVFMGPSTTEYSFPSALCGMTQVASAVAVCGLGEREEVTYSVAPSDLLPTPGASEERSSIGSLVTEESTELGKEAIAEALLREATLVLARPNAYSSVGELLESVNRRIIETTSKTQMLCTENVRRNELAHTLSNVILKHSIDELHQKNIMAHPTEERHPGGTLNTLMESVNQLLHNVMCFTFRKMNHIVTLGEHPAGLSKETVTWTESGPLGCQSFDKAAGHAWVKASACSSSHPSSNAHGTGLVIRDLVEDASPKPDKGGARPELVNNPRLQSEFSCSHRMLDSTARTFPKEMYPKGIVGEDTRNPLHTLSYDSSEQRTSTDIGKLTTVGEVRSTFQESEDSIVPKAQEKHTCATTLNNEAQINLSLLGDDLVVPDQSTLEAKQSEVYGITNFAEELAETVVSMATEIAAICLDNSHGKQPWFCAWKRGNEFLMTPNASCRSLKRKKENSGTGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSTVDGVWQTQSCSRNRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSILNSAIQQACQKNDHLNVRPSCPSKQSSTESITEEFYKYMLRDIAKENKDGALSRRSSHDWSTGLLSPSARSPLCYRQSSMPDSRSPCSRLTVNAPIKANSLDGFAQNCPQDSINVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDGEAANASPGPVSGGSPSQVEKCANRLVTGTGHKGPALLVQESVDYQRKDAVTEGNCSPVSSPSKMAPVKKPSGFDPTRETSACHNAVALKSPRRSLCSREVPLIQIETDQKEECVGESETLLPQSGSLEEAEGPQPEETIPDVARSEDTALSACQSSHDSLETREELEVDVLKEDITLDESRNPPSSSEESTGSWSQLANEEDNPDDTSSFLQLSERSMSNGNTSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESVKERASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVHQKSWKVGDIFHAVVQYCKMHAEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_017596484.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596484.1

ORF Insert Sequence:

ATGTTTCTCA GGATGCTCCT GGGACACAGA GGACACAACG TGGAATCACC ACTGATGCCC 
GAGGACTCGG AGCCACAGCA GATCACCAGC AGTGCAGCAG GCAGCCTGGC TGGCTCCATC 
ACGGCCTGTA AGAAGGTTCT TCGAAGCAAT AGCTTGCTGG AGTCCACAGA CTACTGGTTG 
CAGAACCAGA GGACGCCTTG CCAGATTGGG TTTGTGGAAG ACGAGTCTGA AAACTGTGCT 
TCTGTCTGCT TTGTGAACCT GGATGTCAAC AAGGATGGCT GCAGCACAGA GAACCTGCAG 
CAGAAACTGG TCAACGTTTC ACCAGACCTT CCAAAACTCA TCAACTCCAT GAATGTCCAG 
AAGCCAAAAG AAAATGAAAT TGTCCTCCTA AGTGGGCTAG CCTCTGGAAA CCGCCAGGCA 
GATTTTGATG TTTCACAGTG CCCTTGGCTA CCAGATATCT GCTTGGTCCA GTGCGCAAGA 
GGGAACAGAC CAAACAGTAC CAACTGCATC ATCTTTGAGA TCAACAAGTT CTTAATTGGT 
CTGGAAGTGG TGCAGGAGCG ACAGCTCCAC CTGGAGACCA GCGTGTTGAA GCTGGAGGAT 
GATACAAACT GTTCCCTGTC TTCGATTGAA GAAGATTTCC TTACAGCCTC AGAGCATTTG 
GAGGAAGAAA TCGAGGTGGA AGACTGCAAA AGTGGTTTGG AAACTATAAA TGTGTCAGCT 
AACGTTCTGG AAAGCAAAAG GCCAAAGGAA GCTACCCAGG AAGGATGGGA TTACCACAAG 
GAAATATTGC ATTGTGCTTT AGGTGAAAAA CACATCAGAA AACACCGTAT GCCCTCAATG 
AAAACAGAAA GGTCAAAGGA AAACACAGCA GAGAACACAG CCCTGCAAAG TCTCAATCCT 
TCAGCCAGAC CATCACGCTT GAAAAGTGAA GTGGCGGGGA GCAAGCAGCC AGCCACGAAC 
TACTCTTACC CCGAAAATAT CAGAGGAGAA ATAGAAACAT CTCAGATGCT GTTCATCCCC 
AGGGATGCCT ATCTCTCCAT GGTTTCTTCA TGTGGTGTAC TCACTGAGCA GGGAAGCAAC 
CACAGAGACC ATGACGCCAC TCCAAACTCC CTTCCTCCCG TTCAAAATGG AGAAGCCACC 
GCGGGCGAGT ACGCTACCAA CTTAGCAGAA TCTGTAATGC AAGATGCCTT CATCCGATTG 
TCTCAGTCGC AACCTACACT CCCCCAGGAA TCTGCAGTCA GTTTCTCTGT GGGAAGCGCT 
CTGCTCCCCA GTGGTTGTTG CACCAAAGAT ATGGTGGTCC CTCGTTCATG GAATGAGCTT 
CCTAAAATTG TCATCGTGCA GAGCCCAGAG GGCAGCGACA CAGCCCCTGA GCCAAGTGTC 
TCCTCCTGGC CCGACCTGGA AGTGTCTGTC GAGACCCCGG GCATCTTTTC TGAAGAGAGC 
TCCAGCAGAC CCACTCAGAG TGCCCTAGAA GTTGCTTTGG CTTGTGCAGC CACTGTGATT 
GGAACCACTT CCAGTCCACA GGCCACAGAA AGATTCACCA TGGAGCAAGA ATCTCTGGTA 
TCTACCTACA CCCAGAGAGG CAATGGGATA CAGCAAACTC CAGTGCCCAG AGTATTCATG 
GGACCTTCTA CAACTGAATA CTCCTTCCCA TCAGCTCTGT GTGGCATGAC CCAAGTGGCA 
AGCGCTGTTG CTGTCTGTGG CCTAGGGGAA AGAGAAGAGG TGACATATTC TGTAGCTCCA 
AGTGACCTCC TGCCCACACC TGGGGCTTCT GAAGAAAGAT CCTCCATTGG AAGTTTAGTG 
ACAGAGGAAA GCACAGAACT GGGGAAGGAA GCCATTGCAG AGGCTCTGCT CAGGGAGGCT 
ACTCTGGTTT TAGCGAGGCC GAATGCATAC AGCAGCGTCG GAGAGCTCTT GGAATCTGTG 
AACCGGAGGA TCATAGAAAC CACTTCTAAA ACCCAGATGC TGTGCACAGA AAATGTTCGA 
AGGAATGAAC TGGCACACAC CTTGTCCAAT GTCATCCTCA AGCATTCCAT TGATGAGCTG 
CACCAGAAAA ATATCATGGC TCACCCCACC GAGGAAAGGC ATCCCGGTGG GACTCTGAAC 
ACCTTAATGG AAAGTGTGAA CCAGTTGCTG CACAACGTGA TGTGCTTCAC CTTCAGAAAG 
ATGAATCACA TCGTAACACT GGGTGAGCAT CCCGCTGGCC TTTCTAAGGA GACTGTCACT 
TGGACAGAGT CAGGGCCCTT AGGTTGCCAG TCATTTGATA AGGCTGCTGG TCATGCATGG 
GTTAAAGCCT CGGCTTGCTC CAGCAGCCAT CCCTCTAGCA ATGCACACGG TACTGGCCTT 
GTCATCAGAG ATCTTGTAGA AGATGCATCT CCTAAGCCAG ACAAGGGAGG AGCAAGGCCA 
GAACTGGTCA ACAACCCCAG GCTGCAATCT GAATTCTCAT GCAGTCACAG AATGCTTGAT 
TCAACTGCTA GGACATTCCC CAAGGAAATG TATCCAAAAG GGATTGTGGG AGAGGATACA 
AGAAACCCTC TTCATACACT AAGTTATGAC AGCAGTGAAC AAAGAACTTC TACAGATATA 
GGAAAATTAA CAACAGTAGG AGAGGTCCGT AGTACCTTCC AGGAATCTGA AGACAGCATT 
GTTCCAAAAG CCCAAGAGAA ACACACTTGT GCCACAACTC TAAACAACGA AGCTCAAATT 
AACCTATCCT TGTTAGGGGA TGACCTAGTG GTTCCTGATC AATCTACACT AGAGGCAAAG 
CAGTCCGAGG TCTATGGCAT CACAAACTTT GCGGAAGAAT TGGCAGAGAC TGTTGTCTCC 
ATGGCAACTG AAATCGCAGC AATCTGCCTT GACAACTCCC ATGGAAAACA GCCGTGGTTT 
TGTGCTTGGA AGAGAGGGAA CGAATTTCTG ATGACGCCTA ACGCATCCTG CCGATCCTTG 
AAGAGGAAGA AGGAAAACTC GGGCACGGGA AGCACTGTGA GGAAACACAA GCCACCTCGC 
CTCAGTGAGA TCAAGAGAAA AGCAGATGAG CACCCAGAGC TGAAAGAGAA GCTGATGAAC 
AGAGTCATGG ATGAGTCCAT GAACCTCGAA GACATTCCCG ATTCCGTCAG TACTTTTGCA 
AATGAAGTGG CAGCCAAGAT CATGAACCTA ACAGAATTCT CCACGGTGGA TGGAGTGTGG 
CAAACCCAGA GTTGTTCCCG GAATCGGCTT CTGGGTGGTG ATAGGTGGAA CCGGCTGAAG 
GCCTCGAGCT GTGAAAGTAT TCCGGAGGAG GACTCTGAAG CCAGGGTCTT TGTAAACAGC 
CTGGGTTTAA TGAGTACCTT AAGCCAACCC GTTAGCAGGG CCAGCTCAGT CTCCAAACAG 
TCCAGCTGTG AGAGCATCAC CGATGAGTTT TCCAGGTTCA TGGTGAAACA GATGGAAAAC 
GAAGGGAGGG GGTTCGAGTT GCTGCTGGAT TACTACGCTG GCAAAAATGC CAGCAGTATC 
CTGAACTCAG CAATCCAGCA GGCATGCCAG AAAAACGACC ACCTCAACGT AAGACCAAGC 
TGCCCCTCTA AGCAGTCCAG CACAGAAAGC ATAACAGAGG AGTTCTATAA GTACATGCTG 
AGAGACATTG CCAAAGAAAA CAAAGATGGC GCCCTGTCCA GACGAAGCAG CCATGATTGG 
AGCACGGGCT TGCTGTCTCC TTCCGCGCGA TCCCCACTGT GTTACAGGCA GTCATCTATG 
CCTGACAGCA GGTCCCCGTG CTCCAGGTTA ACAGTAAATG CACCTATCAA AGCCAACTCC 
TTAGATGGTT TTGCCCAAAA CTGCCCTCAA GATTCTATAA ATGTACAGCC AGTCAGTAGG 
GCTTCCTCGT CTGGCCTCTG CAAATCAGAC TCCTGTTTAT ATCGCCGAAG TGGGACTGAC 
CAGATCACAA ACATGCTAAT TCATGAAACA TGGGCCAGCT CCATTGAGGC TCTCATGAGA 
AAGAACAAAA TCATTGCTGA TGACGGCGAG GCAGCTAACG CTAGCCCTGG TCCTGTTTCT 
GGCGGCTCGC CTTCGCAAGT AGAGAAGTGT GCCAACAGAC TAGTCACCGG CACAGGACAC 
AAGGGACCAG CTCTACTTGT ACAGGAGTCT GTTGATTACC AGAGAAAGGA TGCTGTCACT 
GAAGGCAATT GTTCCCCAGT GTCATCTCCA AGCAAAATGG CTCCTGTTAA AAAGCCCAGT 
GGTTTTGATC CTACAAGAGA AACCTCTGCA TGCCACAATG CTGTTGCTCT CAAAAGCCCC 
AGGCGGTCTC TTTGCTCAAG GGAGGTGCCT TTGATTCAGA TAGAGACCGA TCAGAAAGAA 
GAATGCGTTG GAGAATCTGA AACCTTACTT CCCCAAAGTG GCTCCCTAGA GGAAGCAGAA 
GGGCCCCAGC CTGAGGAAAC CATCCCAGAT GTGGCCAGAA GCGAAGACAC AGCCCTGAGC 
GCCTGTCAAA GCTCTCATGA CAGCCTTGAG ACCAGAGAAG AATTAGAAGT GGATGTCTTG 
AAAGAAGACA TAACCCTAGA TGAGTCCCGG AACCCTCCTA GCAGCAGCGA AGAGAGTACA 
GGCAGCTGGT CCCAGCTGGC CAATGAGGAG GACAACCCAG ATGACACAAG TAGCTTTCTG 
CAGCTCAGTG AGCGGTCCAT GAGCAATGGC AACACTAGTG GCACTAGCAG TCTTGGCATT 
ATGGACCTGG ACATTTATCA GGAAAGCATA CCGTCTTCTC CCATGATTAA TGAATTAGTA 
GAAGAAAAGG AGATTCTTAA AGAACAATCA GAAAGCGTAA AGGAACGTGC CTCTGGACTG 
CCAGGGAGAG CAGCCAGCCC CCAGAGGAGT CTACTGGTGA TCAACTTTGA CCTGGAGCCA 
GAGTGTCCTG ATGCTGAGCT GCGAGCCACT CTGCAGTGGA TAGCTGCCTC CGAGCTGGGC 
ATCCCAACAA TCTACTTTAA GAAATCTCAG GAAAGCAGAA TTGAAAAGTT TCTAGATGTT 
GTGAAGCTTG TTCATCAGAA GTCCTGGAAG GTGGGAGATA TATTTCATGC CGTTGTCCAG 
TACTGCAAAA TGCATGCAGA GCAGAAAGAG GGAACTCCGA GTCTCTTTGA CTGGCTCCTG 
GAACTAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( NM_001127492.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001120964.1 Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), mRNA.

Price & Availability↑

CloneID	ORa01680
Clone ID Related Accession (Same CDS sequence)	NM_001127492.1
Accession Version	NM_001127492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-10
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein SPHKAP
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07068191.1. On or before May 22, 2008 this sequence version replaced XM_001062329.1, XM_237334.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCCGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC CCGAGGACTC GGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAGCCTG 
GCTGGCTCCA TCACGGCCTG TAAGAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGT TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGACGAGTCT 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAGGATGG CTGCAGCACA 
GAGAACCTGC AGCAGAAACT GGTCAACGTT TCACCAGACC TTCCAAAACT CATCAACTCC 
ATGAATGTCC AGAAGCCAAA AGAAAATGAA ATTGTCCTCC TAAGTGGGCT AGCCTCTGGA 
AACCGCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGCGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT GGTGCAGGAG CGACAGCTCC ACCTGGAGAC CAGCGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCCCTG TCTTCGATTG AAGAAGATTT CCTTACAGCC 
TCAGAGCATT TGGAGGAAGA AATCGAGGTG GAAGACTGCA AAAGTGGTTT GGAAACTATA 
AATGTGTCAG CTAACGTTCT GGAAAGCAAA AGGCCAAAGG AAGCTACCCA GGAAGGATGG 
GATTACCACA AGGAAATATT GCATTGTGCT TTAGGTGAAA AACACATCAG AAAACACCGT 
ATGCCCTCAA TGAAAACAGA AAGGTCAAAG GAAAACACAG CAGAGAACAC AGCCCTGCAA 
AGTCTCAATC CTTCAGCCAG ACCATCACGC TTGAAAAGTG AAGTGGCGGG GAGCAAGCAG 
CCAGCCACGA ACTACTCTTA CCCCGAAAAT ATCAGAGGAG AAATAGAAAC ATCTCAGATG 
CTGTTCATCC CCAGGGATGC CTATCTCTCC ATGGTTTCTT CATGTGGTGT ACTCACTGAG 
CAGGGAAGCA ACCACAGAGA CCATGACGCC ACTCCAAACT CCCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA CCGCGGGCGA GTACGCTACC AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC GCAACCTACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
GTGGGAAGCG CTCTGCTCCC CAGTGGTTGT TGCACCAAAG ATATGGTGGT CCCTCGTTCA 
TGGAATGAGC TTCCTAAAAT TGTCATCGTG CAGAGCCCAG AGGGCAGCGA CACAGCCCCT 
GAGCCAAGTG TCTCCTCCTG GCCCGACCTG GAAGTGTCTG TCGAGACCCC GGGCATCTTT 
TCTGAAGAGA GCTCCAGCAG ACCCACTCAG AGTGCCCTAG AAGTTGCTTT GGCTTGTGCA 
GCCACTGTGA TTGGAACCAC TTCCAGTCCA CAGGCCACAG AAAGATTCAC CATGGAGCAA 
GAATCTCTGG TATCTACCTA CACCCAGAGA GGCAATGGGA TACAGCAAAC TCCAGTGCCC 
AGAGTATTCA TGGGACCTTC TACAACTGAA TACTCCTTCC CATCAGCTCT GTGTGGCATG 
ACCCAAGTGG CAAGCGCTGT TGCTGTCTGT GGCCTAGGGG AAAGAGAAGA GGTGACATAT 
TCTGTAGCTC CAAGTGACCT CCTGCCCACA CCTGGGGCTT CTGAAGAAAG ATCCTCCATT 
GGAAGTTTAG TGACAGAGGA AAGCACAGAA CTGGGGAAGG AAGCCATTGC AGAGGCTCTG 
CTCAGGGAGG CTACTCTGGT TTTAGCGAGG CCGAATGCAT ACAGCAGCGT CGGAGAGCTC 
TTGGAATCTG TGAACCGGAG GATCATAGAA ACCACTTCTA AAACCCAGAT GCTGTGCACA 
GAAAATGTTC GAAGGAATGA ACTGGCACAC ACCTTGTCCA ATGTCATCCT CAAGCATTCC 
ATTGATGAGC TGCACCAGAA AAATATCATG GCTCACCCCA CCGAGGAAAG GCATCCCGGT 
GGGACTCTGA ACACCTTAAT GGAAAGTGTG AACCAGTTGC TGCACAACGT GATGTGCTTC 
ACCTTCAGAA AGATGAATCA CATCGTAACA CTGGGTGAGC ATCCCTCATT TGATAAGGCT 
GCTGGTCATG CATGGGTTAA AGCCTCGGCT TGCTCCAGCA GCCATCCCTC TAGCAATGCA 
CACGGTACTG GCCTTGTCAT CAGAGATCTT GTAGAAGATG CATCTCCTAA GCCAGACAAG 
GGAGGAGCAA GGCCAGAACT GGTCAACAAC CCCAGGCTGC AATCTGAATT CTCATGCAGT 
CACAGAATGC TTGATTCAAC TGCTAGGACA TTCCCCAAGG AAATGTATCC AAAAGGGATT 
GTGGGAGAGG ATACAAGAAA CCCTCTTCAT ACACTAAGTT ATGACAGCAG TGAACAAAGA 
ACTTCTACAG ATATAGGAAA ATTAACAACA GTAGGAGAGG TCCGTAGTAC CTTCCAGGAA 
TCTGAAGACA GCATTGTTCC AAAAGCCCAA GAGAAACACA CTTGTGCCAC AACTCTAAAC 
AACGAAGCTC AAATTAACCT ATCCTTGTTA GGGGATGACC TAGTGGTTCC TGATCAATCT 
ACACTAGAGG CAAAGCAGTC CGAGGTCTAT GGCATCACAA ACTTTGCGGA AGAATTGGCA 
GAGACTGTTG TCTCCATGGC AACTGAAATC GCAGCAATCT GCCTTGACAA CTCCCATGGA 
AAACAGCCGT GGTTTTGTGC TTGGAAGAGA GGGAACGAAT TTCTGATGAC GCCTAACGCA 
TCCTGCCGAT CCTTGAAGAG GAAGAAGGAA AACTCGGGCA CGGGAAGCAC TGTGAGGAAA 
CACAAGCCAC CTCGCCTCAG TGAGATCAAG AGAAAAGCAG ATGAGCACCC AGAGCTGAAA 
GAGAAGCTGA TGAACAGAGT CATGGATGAG TCCATGAACC TCGAAGACAT TCCCGATTCC 
GTCAGTACTT TTGCAAATGA AGTGGCAGCC AAGATCATGA ACCTAACAGA ATTCTCCACG 
GTGGATGGAG TGTGGCAAAC CCAGAGTTGT TCCCGGAATC GGCTTCTGGG TGGTGATAGG 
TGGAACCGGC TGAAGGCCTC GAGCTGTGAA AGTATTCCGG AGGAGGACTC TGAAGCCAGG 
GTCTTTGTAA ACAGCCTGGG TTTAATGAGT ACCTTAAGCC AACCCGTTAG CAGGGCCAGC 
TCAGTCTCCA AACAGTCCAG CTGTGAGAGC ATCACCGATG AGTTTTCCAG GTTCATGGTG 
AAACAGATGG AAAACGAAGG GAGGGGGTTC GAGTTGCTGC TGGATTACTA CGCTGGCAAA 
AATGCCAGCA GTATCCTGAA CTCAGCAATC CAGCAGGCAT GCCAGAAAAA CGACCACCTC 
AACGTAAGAC CAAGCTGCCC CTCTAAGCAG TCCAGCACAG AAAGCATAAC AGAGGAGTTC 
TATAAGTACA TGCTGAGAGA CATTGCCAAA GAAAACAAAG ATGGCGCCCT GTCCAGACGA 
AGCAGCCATG ATTGGAGCAC GGGCTTGCTG TCTCCTTCCG CGCGATCCCC ACTGTGTTAC 
AGGCAGTCAT CTATGCCTGA CAGCAGGTCC CCGTGCTCCA GGTTAACAGT AAATGCACCT 
ATCAAAGCCA ACTCCTTAGA TGGTTTTGCC CAAAACTGCC CTCAAGATTC TATAAATGTA 
CAGCCAGTCA GTAGGGCTTC CTCGTCTGGC CTCTGCAAAT CAGACTCCTG TTTATATCGC 
CGAAGTGGGA CTGACCAGAT CACAAACATG CTAATTCATG AAACATGGGC CAGCTCCATT 
GAGGCTCTCA TGAGAAAGAA CAAAATCATT GCTGATGACG GCGAGGCAGC TAACGCTAGC 
CCTGGTCCTG TTTCTGGCGG CTCGCCTTCG CAAGTAGAGA AGTGTGCCAA CAGACTAGTC 
ACCGGCACAG GACACAAGGG ACCAGCTCTA CTTGTACAGG AGTCTGTTGA TTACCAGAGA 
AAGGATGCTG TCACTGAAGG CAATTGTTCC CCAGTGTCAT CTCCAAGCAA AATGGCTCCT 
GTTAAAAAGC CCAGTGGTTT TGATCCTACA AGAGAAACCT CTGCATGCCA CAATGCTGTT 
GCTCTCAAAA GCCCCAGGCG GTCTCTTTGC TCAAGGGAGG TGCCTTTGAT TCAGATAGAG 
ACCGATCAGA AAGAAGAATG CGTTGGAGAA TCTGAAACCT TACTTCCCCA AAGTGGCTCC 
CTAGAGGAAG CAGAAGGGCC CCAGCCTGAG GAAACCATCC CAGATGTGGC CAGAAGCGAA 
GACACAGCCC TGAGCGCCTG TCAAAGCTCT CATGACAGCC TTGAGACCAG AGAAGAATTA 
GAAGTGGATG TCTTGAAAGA AGACATAACC CTAGATGAGT CCCGGAACCC TCCTAGCAGC 
AGCGAAGAGA GTACAGGCAG CTGGTCCCAG CTGGCCAATG AGGAGGACAA CCCAGATGAC 
ACAAGTAGCT TTCTGCAGCT CAGTGAGCGG TCCATGAGCA ATGGCAACAC TAGTGGCACT 
AGCAGTCTTG GCATTATGGA CCTGGACATT TATCAGGAAA GCATACCGTC TTCTCCCATG 
ATTAATGAAT TAGTAGAAGA AAAGGAGATT CTTAAAGAAC AATCAGAAAG CGTAAAGGAA 
CGTGCCTCTG GACTGCCAGG GAGAGCAGCC AGCCCCCAGA GGAGTCTACT GGTGATCAAC 
TTTGACCTGG AGCCAGAGTG TCCTGATGCT GAGCTGCGAG CCACTCTGCA GTGGATAGCT 
GCCTCCGAGC TGGGCATCCC AACAATCTAC TTTAAGAAAT CTCAGGAAAG CAGAATTGAA 
AAGTTTCTAG ATGTTGTGAA GCTTGTTCAT CAGAAGTCCT GGAAGGTGGG AGATATATTT 
CATGCCGTTG TCCAGTACTG CAAAATGCAT GCAGAGCAGA AAGAGGGAAC TCCGAGTCTC 
TTTGACTGGC TCCTGGAACT AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120964.1
CDS	151..5277
Translation	MSTPGFLCKAMWVVEPNSATCLSAAPPVKRHCQESSNVESPLMPEDSEPQQITSSAAGSLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDGCSTENLQQKLVNVSPDLPKLINSMNVQKPKENEIVLLSGLASGNRQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETSVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVEDCKSGLETINVSANVLESKRPKEATQEGWDYHKEILHCALGEKHIRKHRMPSMKTERSKENTAENTALQSLNPSARPSRLKSEVAGSKQPATNYSYPENIRGEIETSQMLFIPRDAYLSMVSSCGVLTEQGSNHRDHDATPNSLPPVQNGEATAGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSVGSALLPSGCCTKDMVVPRSWNELPKIVIVQSPEGSDTAPEPSVSSWPDLEVSVETPGIFSEESSSRPTQSALEVALACAATVIGTTSSPQATERFTMEQESLVSTYTQRGNGIQQTPVPRVFMGPSTTEYSFPSALCGMTQVASAVAVCGLGEREEVTYSVAPSDLLPTPGASEERSSIGSLVTEESTELGKEAIAEALLREATLVLARPNAYSSVGELLESVNRRIIETTSKTQMLCTENVRRNELAHTLSNVILKHSIDELHQKNIMAHPTEERHPGGTLNTLMESVNQLLHNVMCFTFRKMNHIVTLGEHPSFDKAAGHAWVKASACSSSHPSSNAHGTGLVIRDLVEDASPKPDKGGARPELVNNPRLQSEFSCSHRMLDSTARTFPKEMYPKGIVGEDTRNPLHTLSYDSSEQRTSTDIGKLTTVGEVRSTFQESEDSIVPKAQEKHTCATTLNNEAQINLSLLGDDLVVPDQSTLEAKQSEVYGITNFAEELAETVVSMATEIAAICLDNSHGKQPWFCAWKRGNEFLMTPNASCRSLKRKKENSGTGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSTVDGVWQTQSCSRNRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSILNSAIQQACQKNDHLNVRPSCPSKQSSTESITEEFYKYMLRDIAKENKDGALSRRSSHDWSTGLLSPSARSPLCYRQSSMPDSRSPCSRLTVNAPIKANSLDGFAQNCPQDSINVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDGEAANASPGPVSGGSPSQVEKCANRLVTGTGHKGPALLVQESVDYQRKDAVTEGNCSPVSSPSKMAPVKKPSGFDPTRETSACHNAVALKSPRRSLCSREVPLIQIETDQKEECVGESETLLPQSGSLEEAEGPQPEETIPDVARSEDTALSACQSSHDSLETREELEVDVLKEDITLDESRNPPSSSEESTGSWSQLANEEDNPDDTSSFLQLSERSMSNGNTSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESVKERASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVHQKSWKVGDIFHAVVQYCKMHAEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	NM_001127492.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127492.1

ORF Insert Sequence:

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCCGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC CCGAGGACTC GGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAGCCTG 
GCTGGCTCCA TCACGGCCTG TAAGAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGT TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGACGAGTCT 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAGGATGG CTGCAGCACA 
GAGAACCTGC AGCAGAAACT GGTCAACGTT TCACCAGACC TTCCAAAACT CATCAACTCC 
ATGAATGTCC AGAAGCCAAA AGAAAATGAA ATTGTCCTCC TAAGTGGGCT AGCCTCTGGA 
AACCGCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGCGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT GGTGCAGGAG CGACAGCTCC ACCTGGAGAC CAGCGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCCCTG TCTTCGATTG AAGAAGATTT CCTTACAGCC 
TCAGAGCATT TGGAGGAAGA AATCGAGGTG GAAGACTGCA AAAGTGGTTT GGAAACTATA 
AATGTGTCAG CTAACGTTCT GGAAAGCAAA AGGCCAAAGG AAGCTACCCA GGAAGGATGG 
GATTACCACA AGGAAATATT GCATTGTGCT TTAGGTGAAA AACACATCAG AAAACACCGT 
ATGCCCTCAA TGAAAACAGA AAGGTCAAAG GAAAACACAG CAGAGAACAC AGCCCTGCAA 
AGTCTCAATC CTTCAGCCAG ACCATCACGC TTGAAAAGTG AAGTGGCGGG GAGCAAGCAG 
CCAGCCACGA ACTACTCTTA CCCCGAAAAT ATCAGAGGAG AAATAGAAAC ATCTCAGATG 
CTGTTCATCC CCAGGGATGC CTATCTCTCC ATGGTTTCTT CATGTGGTGT ACTCACTGAG 
CAGGGAAGCA ACCACAGAGA CCATGACGCC ACTCCAAACT CCCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA CCGCGGGCGA GTACGCTACC AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC GCAACCTACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
GTGGGAAGCG CTCTGCTCCC CAGTGGTTGT TGCACCAAAG ATATGGTGGT CCCTCGTTCA 
TGGAATGAGC TTCCTAAAAT TGTCATCGTG CAGAGCCCAG AGGGCAGCGA CACAGCCCCT 
GAGCCAAGTG TCTCCTCCTG GCCCGACCTG GAAGTGTCTG TCGAGACCCC GGGCATCTTT 
TCTGAAGAGA GCTCCAGCAG ACCCACTCAG AGTGCCCTAG AAGTTGCTTT GGCTTGTGCA 
GCCACTGTGA TTGGAACCAC TTCCAGTCCA CAGGCCACAG AAAGATTCAC CATGGAGCAA 
GAATCTCTGG TATCTACCTA CACCCAGAGA GGCAATGGGA TACAGCAAAC TCCAGTGCCC 
AGAGTATTCA TGGGACCTTC TACAACTGAA TACTCCTTCC CATCAGCTCT GTGTGGCATG 
ACCCAAGTGG CAAGCGCTGT TGCTGTCTGT GGCCTAGGGG AAAGAGAAGA GGTGACATAT 
TCTGTAGCTC CAAGTGACCT CCTGCCCACA CCTGGGGCTT CTGAAGAAAG ATCCTCCATT 
GGAAGTTTAG TGACAGAGGA AAGCACAGAA CTGGGGAAGG AAGCCATTGC AGAGGCTCTG 
CTCAGGGAGG CTACTCTGGT TTTAGCGAGG CCGAATGCAT ACAGCAGCGT CGGAGAGCTC 
TTGGAATCTG TGAACCGGAG GATCATAGAA ACCACTTCTA AAACCCAGAT GCTGTGCACA 
GAAAATGTTC GAAGGAATGA ACTGGCACAC ACCTTGTCCA ATGTCATCCT CAAGCATTCC 
ATTGATGAGC TGCACCAGAA AAATATCATG GCTCACCCCA CCGAGGAAAG GCATCCCGGT 
GGGACTCTGA ACACCTTAAT GGAAAGTGTG AACCAGTTGC TGCACAACGT GATGTGCTTC 
ACCTTCAGAA AGATGAATCA CATCGTAACA CTGGGTGAGC ATCCCTCATT TGATAAGGCT 
GCTGGTCATG CATGGGTTAA AGCCTCGGCT TGCTCCAGCA GCCATCCCTC TAGCAATGCA 
CACGGTACTG GCCTTGTCAT CAGAGATCTT GTAGAAGATG CATCTCCTAA GCCAGACAAG 
GGAGGAGCAA GGCCAGAACT GGTCAACAAC CCCAGGCTGC AATCTGAATT CTCATGCAGT 
CACAGAATGC TTGATTCAAC TGCTAGGACA TTCCCCAAGG AAATGTATCC AAAAGGGATT 
GTGGGAGAGG ATACAAGAAA CCCTCTTCAT ACACTAAGTT ATGACAGCAG TGAACAAAGA 
ACTTCTACAG ATATAGGAAA ATTAACAACA GTAGGAGAGG TCCGTAGTAC CTTCCAGGAA 
TCTGAAGACA GCATTGTTCC AAAAGCCCAA GAGAAACACA CTTGTGCCAC AACTCTAAAC 
AACGAAGCTC AAATTAACCT ATCCTTGTTA GGGGATGACC TAGTGGTTCC TGATCAATCT 
ACACTAGAGG CAAAGCAGTC CGAGGTCTAT GGCATCACAA ACTTTGCGGA AGAATTGGCA 
GAGACTGTTG TCTCCATGGC AACTGAAATC GCAGCAATCT GCCTTGACAA CTCCCATGGA 
AAACAGCCGT GGTTTTGTGC TTGGAAGAGA GGGAACGAAT TTCTGATGAC GCCTAACGCA 
TCCTGCCGAT CCTTGAAGAG GAAGAAGGAA AACTCGGGCA CGGGAAGCAC TGTGAGGAAA 
CACAAGCCAC CTCGCCTCAG TGAGATCAAG AGAAAAGCAG ATGAGCACCC AGAGCTGAAA 
GAGAAGCTGA TGAACAGAGT CATGGATGAG TCCATGAACC TCGAAGACAT TCCCGATTCC 
GTCAGTACTT TTGCAAATGA AGTGGCAGCC AAGATCATGA ACCTAACAGA ATTCTCCACG 
GTGGATGGAG TGTGGCAAAC CCAGAGTTGT TCCCGGAATC GGCTTCTGGG TGGTGATAGG 
TGGAACCGGC TGAAGGCCTC GAGCTGTGAA AGTATTCCGG AGGAGGACTC TGAAGCCAGG 
GTCTTTGTAA ACAGCCTGGG TTTAATGAGT ACCTTAAGCC AACCCGTTAG CAGGGCCAGC 
TCAGTCTCCA AACAGTCCAG CTGTGAGAGC ATCACCGATG AGTTTTCCAG GTTCATGGTG 
AAACAGATGG AAAACGAAGG GAGGGGGTTC GAGTTGCTGC TGGATTACTA CGCTGGCAAA 
AATGCCAGCA GTATCCTGAA CTCAGCAATC CAGCAGGCAT GCCAGAAAAA CGACCACCTC 
AACGTAAGAC CAAGCTGCCC CTCTAAGCAG TCCAGCACAG AAAGCATAAC AGAGGAGTTC 
TATAAGTACA TGCTGAGAGA CATTGCCAAA GAAAACAAAG ATGGCGCCCT GTCCAGACGA 
AGCAGCCATG ATTGGAGCAC GGGCTTGCTG TCTCCTTCCG CGCGATCCCC ACTGTGTTAC 
AGGCAGTCAT CTATGCCTGA CAGCAGGTCC CCGTGCTCCA GGTTAACAGT AAATGCACCT 
ATCAAAGCCA ACTCCTTAGA TGGTTTTGCC CAAAACTGCC CTCAAGATTC TATAAATGTA 
CAGCCAGTCA GTAGGGCTTC CTCGTCTGGC CTCTGCAAAT CAGACTCCTG TTTATATCGC 
CGAAGTGGGA CTGACCAGAT CACAAACATG CTAATTCATG AAACATGGGC CAGCTCCATT 
GAGGCTCTCA TGAGAAAGAA CAAAATCATT GCTGATGACG GCGAGGCAGC TAACGCTAGC 
CCTGGTCCTG TTTCTGGCGG CTCGCCTTCG CAAGTAGAGA AGTGTGCCAA CAGACTAGTC 
ACCGGCACAG GACACAAGGG ACCAGCTCTA CTTGTACAGG AGTCTGTTGA TTACCAGAGA 
AAGGATGCTG TCACTGAAGG CAATTGTTCC CCAGTGTCAT CTCCAAGCAA AATGGCTCCT 
GTTAAAAAGC CCAGTGGTTT TGATCCTACA AGAGAAACCT CTGCATGCCA CAATGCTGTT 
GCTCTCAAAA GCCCCAGGCG GTCTCTTTGC TCAAGGGAGG TGCCTTTGAT TCAGATAGAG 
ACCGATCAGA AAGAAGAATG CGTTGGAGAA TCTGAAACCT TACTTCCCCA AAGTGGCTCC 
CTAGAGGAAG CAGAAGGGCC CCAGCCTGAG GAAACCATCC CAGATGTGGC CAGAAGCGAA 
GACACAGCCC TGAGCGCCTG TCAAAGCTCT CATGACAGCC TTGAGACCAG AGAAGAATTA 
GAAGTGGATG TCTTGAAAGA AGACATAACC CTAGATGAGT CCCGGAACCC TCCTAGCAGC 
AGCGAAGAGA GTACAGGCAG CTGGTCCCAG CTGGCCAATG AGGAGGACAA CCCAGATGAC 
ACAAGTAGCT TTCTGCAGCT CAGTGAGCGG TCCATGAGCA ATGGCAACAC TAGTGGCACT 
AGCAGTCTTG GCATTATGGA CCTGGACATT TATCAGGAAA GCATACCGTC TTCTCCCATG 
ATTAATGAAT TAGTAGAAGA AAAGGAGATT CTTAAAGAAC AATCAGAAAG CGTAAAGGAA 
CGTGCCTCTG GACTGCCAGG GAGAGCAGCC AGCCCCCAGA GGAGTCTACT GGTGATCAAC 
TTTGACCTGG AGCCAGAGTG TCCTGATGCT GAGCTGCGAG CCACTCTGCA GTGGATAGCT 
GCCTCCGAGC TGGGCATCCC AACAATCTAC TTTAAGAAAT CTCAGGAAAG CAGAATTGAA 
AAGTTTCTAG ATGTTGTGAA GCTTGTTCAT CAGAAGTCCT GGAAGGTGGG AGATATATTT 
CATGCCGTTG TCCAGTACTG CAAAATGCAT GCAGAGCAGA AAGAGGGAAC TCCGAGTCTC 
TTTGACTGGC TCCTGGAACT AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( XM_006245230.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245292.1 Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X1, mRNA.

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CloneID	ORa32395
Clone ID Related Accession (Same CDS sequence)	XM_006245230.3
Accession Version	XM_006245230.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245230.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCCGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC CCGAGGACTC GGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAGCCTG 
GCTGGCTCCA TCACGGCCTG TAAGAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGT TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGACGAGTCT 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAGGATGG CTGCAGCACA 
GAGAACCTGC AGCAGAAACT GGTCAACGTT TCACCAGACC TTCCAAAACT CATCAACTCC 
ATGAATGTCC AGAAGCCAAA AGAAAATGAA ATTGTCCTCC TAAGTGGGCT AGCCTCTGGA 
AACCGCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGCGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT GGTGCAGGAG CGACAGCTCC ACCTGGAGAC CAGCGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCCCTG TCTTCGATTG AAGAAGATTT CCTTACAGCC 
TCAGAGCATT TGGAGGAAGA AATCGAGGTG GAAGACTGCA AAAGTGGTTT GGAAACTATA 
AATGTGTCAG CTAACGTTCT GGAAAGCAAA AGGCCAAAGG AAGCTACCCA GGAAGGATGG 
GATTACCACA AGGAAATATT GCATTGTGCT TTAGGTGAAA AACACATCAG AAAACACCGT 
ATGCCCTCAA TGAAAACAGA AAGGTCAAAG GAAAACACAG CAGAGAACAC AGCCCTGCAA 
AGTCTCAATC CTTCAGCCAG ACCATCACGC TTGAAAAGTG AAGTGGCGGG GAGCAAGCAG 
CCAGCCACGA ACTACTCTTA CCCCGAAAAT ATCAGAGGAG AAATAGAAAC ATCTCAGATG 
CTGTTCATCC CCAGGGATGC CTATCTCTCC ATGGTTTCTT CATGTGGTGT ACTCACTGAG 
CAGGGAAGCA ACCACAGAGA CCATGACGCC ACTCCAAACT CCCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA CCGCGGGCGA GTACGCTACC AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC GCAACCTACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
GTGGGAAGCG CTCTGCTCCC CAGTGGTTGT TGCACCAAAG ATATGGTGGT CCCTCGTTCA 
TGGAATGAGC TTCCTAAAAT TGTCATCGTG CAGAGCCCAG AGGGCAGCGA CACAGCCCCT 
GAGCCAAGTG TCTCCTCCTG GCCCGACCTG GAAGTGTCTG TCGAGACCCC GGGCATCTTT 
TCTGAAGAGA GCTCCAGCAG ACCCACTCAG AGTGCCCTAG AAGTTGCTTT GGCTTGTGCA 
GCCACTGTGA TTGGAACCAC TTCCAGTCCA CAGGCCACAG AAAGATTCAC CATGGAGCAA 
GAATCTCTGG TATCTACCTA CACCCAGAGA GGCAATGGGA TACAGCAAAC TCCAGTGCCC 
AGAGTATTCA TGGGACCTTC TACAACTGAA TACTCCTTCC CATCAGCTCT GTGTGGCATG 
ACCCAAGTGG CAAGCGCTGT TGCTGTCTGT GGCCTAGGGG AAAGAGAAGA GGTGACATAT 
TCTGTAGCTC CAAGTGACCT CCTGCCCACA CCTGGGGCTT CTGAAGAAAG ATCCTCCATT 
GGAAGTTTAG TGACAGAGGA AAGCACAGAA CTGGGGAAGG AAGCCATTGC AGAGGCTCTG 
CTCAGGGAGG CTACTCTGGT TTTAGCGAGG CCGAATGCAT ACAGCAGCGT CGGAGAGCTC 
TTGGAATCTG TGAACCGGAG GATCATAGAA ACCACTTCTA AAACCCAGAT GCTGTGCACA 
GAAAATGTTC GAAGGAATGA ACTGGCACAC ACCTTGTCCA ATGTCATCCT CAAGCATTCC 
ATTGATGAGC TGCACCAGAA AAATATCATG GCTCACCCCA CCGAGGAAAG GCATCCCGGT 
GGGACTCTGA ACACCTTAAT GGAAAGTGTG AACCAGTTGC TGCACAACGT GATGTGCTTC 
ACCTTCAGAA AGATGAATCA CATCGTAACA CTGGGTGAGC ATCCCGCTGG CCTTTCTAAG 
GAGACTGTCA CTTGGACAGA GTCAGGGCCC TTAGGTTGCC AGTCATTTGA TAAGGCTGCT 
GGTCATGCAT GGGTTAAAGC CTCGGCTTGC TCCAGCAGCC ATCCCTCTAG CAATGCACAC 
GGTACTGGCC TTGTCATCAG AGATCTTGTA GAAGATGCAT CTCCTAAGCC AGACAAGGGA 
GGAGCAAGGC CAGAACTGGT CAACAACCCC AGGCTGCAAT CTGAATTCTC ATGCAGTCAC 
AGAATGCTTG ATTCAACTGC TAGGACATTC CCCAAGGAAA TGTATCCAAA AGGGATTGTG 
GGAGAGGATA CAAGAAACCC TCTTCATACA CTAAGTTATG ACAGCAGTGA ACAAAGAACT 
TCTACAGATA TAGGAAAATT AACAACAGTA GGAGAGGTCC GTAGTACCTT CCAGGAATCT 
GAAGACAGCA TTGTTCCAAA AGCCCAAGAG AAACACACTT GTGCCACAAC TCTAAACAAC 
GAAGCTCAAA TTAACCTATC CTTGTTAGGG GATGACCTAG TGGTTCCTGA TCAATCTACA 
CTAGAGGCAA AGCAGTCCGA GGTCTATGGC ATCACAAACT TTGCGGAAGA ATTGGCAGAG 
ACTGTTGTCT CCATGGCAAC TGAAATCGCA GCAATCTGCC TTGACAACTC CCATGGAAAA 
CAGCCGTGGT TTTGTGCTTG GAAGAGAGGG AACGAATTTC TGATGACGCC TAACGCATCC 
TGCCGATCCT TGAAGAGGAA GAAGGAAAAC TCGGGCACGG GAAGCACTGT GAGGAAACAC 
AAGCCACCTC GCCTCAGTGA GATCAAGAGA AAAGCAGATG AGCACCCAGA GCTGAAAGAG 
AAGCTGATGA ACAGAGTCAT GGATGAGTCC ATGAACCTCG AAGACATTCC CGATTCCGTC 
AGTACTTTTG CAAATGAAGT GGCAGCCAAG ATCATGAACC TAACAGAATT CTCCACGGTG 
GATGGAGTGT GGCAAACCCA GAGTTGTTCC CGGAATCGGC TTCTGGGTGG TGATAGGTGG 
AACCGGCTGA AGGCCTCGAG CTGTGAAAGT ATTCCGGAGG AGGACTCTGA AGCCAGGGTC 
TTTGTAAACA GCCTGGGTTT AATGAGTACC TTAAGCCAAC CCGTTAGCAG GGCCAGCTCA 
GTCTCCAAAC AGTCCAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAA 
CAGATGGAAA ACGAAGGGAG GGGGTTCGAG TTGCTGCTGG ATTACTACGC TGGCAAAAAT 
GCCAGCAGTA TCCTGAACTC AGCAATCCAG CAGGCATGCC AGAAAAACGA CCACCTCAAC 
GTAAGACCAA GCTGCCCCTC TAAGCAGTCC AGCACAGAAA GCATAACAGA GGAGTTCTAT 
AAGTACATGC TGAGAGACAT TGCCAAAGAA AACAAAGATG GCGCCCTGTC CAGACGAAGC 
AGCCATGATT GGAGCACGGG CTTGCTGTCT CCTTCCGCGC GATCCCCACT GTGTTACAGG 
CAGTCATCTA TGCCTGACAG CAGGTCCCCG TGCTCCAGGT TAACAGTAAA TGCACCTATC 
AAAGCCAACT CCTTAGATGG TTTTGCCCAA AACTGCCCTC AAGATTCTAT AAATGTACAG 
CCAGTCAGTA GGGCTTCCTC GTCTGGCCTC TGCAAATCAG ACTCCTGTTT ATATCGCCGA 
AGTGGGACTG ACCAGATCAC AAACATGCTA ATTCATGAAA CATGGGCCAG CTCCATTGAG 
GCTCTCATGA GAAAGAACAA AATCATTGCT GATGACGGCG AGGCAGCTAA CGCTAGCCCT 
GGTCCTGTTT CTGGCGGCTC GCCTTCGCAA GTAGAGAAGT GTGCCAACAG ACTAGTCACC 
GGCACAGGAC ACAAGGGACC AGCTCTACTT GTACAGGAGT CTGTTGATTA CCAGAGAAAG 
GATGCTGTCA CTGAAGGCAA TTGTTCCCCA GTGTCATCTC CAAGCAAAAT GGCTCCTGTT 
AAAAAGCCCA GTGGTTTTGA TCCTACAAGA GAAACCTCTG CATGCCACAA TGCTGTTGCT 
CTCAAAAGCC CCAGGCGGTC TCTTTGCTCA AGGGAGGTGC CTTTGATTCA GATAGAGACC 
GATCAGAAAG AAGAATGCGT TGGAGAATCT GAAACCTTAC TTCCCCAAAG TGGCTCCCTA 
GAGGAAGCAG AAGGGCCCCA GCCTGAGGAA ACCATCCCAG ATGTGGCCAG AAGCGAAGAC 
ACAGCCCTGA GCGCCTGTCA AAGCTCTCAT GACAGCCTTG AGACCAGAGA AGAATTAGAA 
GTGGATGTCT TGAAAGAAGA CATAACCCTA GATGAGTCCC GGAACCCTCC TAGCAGCAGC 
GAAGAGAGTA CAGGCAGCTG GTCCCAGCTG GCCAATGAGG AGGACAACCC AGATGACACA 
AGTAGCTTTC TGCAGCTCAG TGAGCGGTCC ATGAGCAATG GCAACACTAG TGGCACTAGC 
AGTCTTGGCA TTATGGACCT GGACATTTAT CAGGAAAGCA TACCGTCTTC TCCCATGATT 
AATGAATTAG TAGAAGAAAA GGAGATTCTT AAAGAACAAT CAGAAAGCGT AAAGGAACGT 
GCCTCTGGAC TGCCAGGGAG AGCAGCCAGC CCCCAGAGGA GTCTACTGGT GATCAACTTT 
GACCTGGAGC CAGAGTGTCC TGATGCTGAG CTGCGAGCCA CTCTGCAGTG GATAGCTGCC 
TCCGAGCTGG GCATCCCAAC AATCTACTTT AAGAAATCTC AGGAAAGCAG AATTGAAAAG 
TTTCTAGATG TTGTGAAGCT TGTTCATCAG AAGTCCTGGA AGGTGGGAGA TATATTTCAT 
GCCGTTGTCC AGTACTGCAA AATGCATGCA GAGCAGAAAG AGGGAACTCC GAGTCTCTTT 
GACTGGCTCC TGGAACTAGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245292.1
CDS	313..5496
Translation	MSTPGFLCKAMWVVEPNSATCLSAAPPVKRHCQESSNVESPLMPEDSEPQQITSSAAGSLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDGCSTENLQQKLVNVSPDLPKLINSMNVQKPKENEIVLLSGLASGNRQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETSVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVEDCKSGLETINVSANVLESKRPKEATQEGWDYHKEILHCALGEKHIRKHRMPSMKTERSKENTAENTALQSLNPSARPSRLKSEVAGSKQPATNYSYPENIRGEIETSQMLFIPRDAYLSMVSSCGVLTEQGSNHRDHDATPNSLPPVQNGEATAGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSVGSALLPSGCCTKDMVVPRSWNELPKIVIVQSPEGSDTAPEPSVSSWPDLEVSVETPGIFSEESSSRPTQSALEVALACAATVIGTTSSPQATERFTMEQESLVSTYTQRGNGIQQTPVPRVFMGPSTTEYSFPSALCGMTQVASAVAVCGLGEREEVTYSVAPSDLLPTPGASEERSSIGSLVTEESTELGKEAIAEALLREATLVLARPNAYSSVGELLESVNRRIIETTSKTQMLCTENVRRNELAHTLSNVILKHSIDELHQKNIMAHPTEERHPGGTLNTLMESVNQLLHNVMCFTFRKMNHIVTLGEHPAGLSKETVTWTESGPLGCQSFDKAAGHAWVKASACSSSHPSSNAHGTGLVIRDLVEDASPKPDKGGARPELVNNPRLQSEFSCSHRMLDSTARTFPKEMYPKGIVGEDTRNPLHTLSYDSSEQRTSTDIGKLTTVGEVRSTFQESEDSIVPKAQEKHTCATTLNNEAQINLSLLGDDLVVPDQSTLEAKQSEVYGITNFAEELAETVVSMATEIAAICLDNSHGKQPWFCAWKRGNEFLMTPNASCRSLKRKKENSGTGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSTVDGVWQTQSCSRNRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSILNSAIQQACQKNDHLNVRPSCPSKQSSTESITEEFYKYMLRDIAKENKDGALSRRSSHDWSTGLLSPSARSPLCYRQSSMPDSRSPCSRLTVNAPIKANSLDGFAQNCPQDSINVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDGEAANASPGPVSGGSPSQVEKCANRLVTGTGHKGPALLVQESVDYQRKDAVTEGNCSPVSSPSKMAPVKKPSGFDPTRETSACHNAVALKSPRRSLCSREVPLIQIETDQKEECVGESETLLPQSGSLEEAEGPQPEETIPDVARSEDTALSACQSSHDSLETREELEVDVLKEDITLDESRNPPSSSEESTGSWSQLANEEDNPDDTSSFLQLSERSMSNGNTSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESVKERASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVHQKSWKVGDIFHAVVQYCKMHAEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006245230.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245230.3

ORF Insert Sequence:

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCCGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC CCGAGGACTC GGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAGCCTG 
GCTGGCTCCA TCACGGCCTG TAAGAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGT TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGACGAGTCT 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAGGATGG CTGCAGCACA 
GAGAACCTGC AGCAGAAACT GGTCAACGTT TCACCAGACC TTCCAAAACT CATCAACTCC 
ATGAATGTCC AGAAGCCAAA AGAAAATGAA ATTGTCCTCC TAAGTGGGCT AGCCTCTGGA 
AACCGCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGCGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT GGTGCAGGAG CGACAGCTCC ACCTGGAGAC CAGCGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCCCTG TCTTCGATTG AAGAAGATTT CCTTACAGCC 
TCAGAGCATT TGGAGGAAGA AATCGAGGTG GAAGACTGCA AAAGTGGTTT GGAAACTATA 
AATGTGTCAG CTAACGTTCT GGAAAGCAAA AGGCCAAAGG AAGCTACCCA GGAAGGATGG 
GATTACCACA AGGAAATATT GCATTGTGCT TTAGGTGAAA AACACATCAG AAAACACCGT 
ATGCCCTCAA TGAAAACAGA AAGGTCAAAG GAAAACACAG CAGAGAACAC AGCCCTGCAA 
AGTCTCAATC CTTCAGCCAG ACCATCACGC TTGAAAAGTG AAGTGGCGGG GAGCAAGCAG 
CCAGCCACGA ACTACTCTTA CCCCGAAAAT ATCAGAGGAG AAATAGAAAC ATCTCAGATG 
CTGTTCATCC CCAGGGATGC CTATCTCTCC ATGGTTTCTT CATGTGGTGT ACTCACTGAG 
CAGGGAAGCA ACCACAGAGA CCATGACGCC ACTCCAAACT CCCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA CCGCGGGCGA GTACGCTACC AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC GCAACCTACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
GTGGGAAGCG CTCTGCTCCC CAGTGGTTGT TGCACCAAAG ATATGGTGGT CCCTCGTTCA 
TGGAATGAGC TTCCTAAAAT TGTCATCGTG CAGAGCCCAG AGGGCAGCGA CACAGCCCCT 
GAGCCAAGTG TCTCCTCCTG GCCCGACCTG GAAGTGTCTG TCGAGACCCC GGGCATCTTT 
TCTGAAGAGA GCTCCAGCAG ACCCACTCAG AGTGCCCTAG AAGTTGCTTT GGCTTGTGCA 
GCCACTGTGA TTGGAACCAC TTCCAGTCCA CAGGCCACAG AAAGATTCAC CATGGAGCAA 
GAATCTCTGG TATCTACCTA CACCCAGAGA GGCAATGGGA TACAGCAAAC TCCAGTGCCC 
AGAGTATTCA TGGGACCTTC TACAACTGAA TACTCCTTCC CATCAGCTCT GTGTGGCATG 
ACCCAAGTGG CAAGCGCTGT TGCTGTCTGT GGCCTAGGGG AAAGAGAAGA GGTGACATAT 
TCTGTAGCTC CAAGTGACCT CCTGCCCACA CCTGGGGCTT CTGAAGAAAG ATCCTCCATT 
GGAAGTTTAG TGACAGAGGA AAGCACAGAA CTGGGGAAGG AAGCCATTGC AGAGGCTCTG 
CTCAGGGAGG CTACTCTGGT TTTAGCGAGG CCGAATGCAT ACAGCAGCGT CGGAGAGCTC 
TTGGAATCTG TGAACCGGAG GATCATAGAA ACCACTTCTA AAACCCAGAT GCTGTGCACA 
GAAAATGTTC GAAGGAATGA ACTGGCACAC ACCTTGTCCA ATGTCATCCT CAAGCATTCC 
ATTGATGAGC TGCACCAGAA AAATATCATG GCTCACCCCA CCGAGGAAAG GCATCCCGGT 
GGGACTCTGA ACACCTTAAT GGAAAGTGTG AACCAGTTGC TGCACAACGT GATGTGCTTC 
ACCTTCAGAA AGATGAATCA CATCGTAACA CTGGGTGAGC ATCCCGCTGG CCTTTCTAAG 
GAGACTGTCA CTTGGACAGA GTCAGGGCCC TTAGGTTGCC AGTCATTTGA TAAGGCTGCT 
GGTCATGCAT GGGTTAAAGC CTCGGCTTGC TCCAGCAGCC ATCCCTCTAG CAATGCACAC 
GGTACTGGCC TTGTCATCAG AGATCTTGTA GAAGATGCAT CTCCTAAGCC AGACAAGGGA 
GGAGCAAGGC CAGAACTGGT CAACAACCCC AGGCTGCAAT CTGAATTCTC ATGCAGTCAC 
AGAATGCTTG ATTCAACTGC TAGGACATTC CCCAAGGAAA TGTATCCAAA AGGGATTGTG 
GGAGAGGATA CAAGAAACCC TCTTCATACA CTAAGTTATG ACAGCAGTGA ACAAAGAACT 
TCTACAGATA TAGGAAAATT AACAACAGTA GGAGAGGTCC GTAGTACCTT CCAGGAATCT 
GAAGACAGCA TTGTTCCAAA AGCCCAAGAG AAACACACTT GTGCCACAAC TCTAAACAAC 
GAAGCTCAAA TTAACCTATC CTTGTTAGGG GATGACCTAG TGGTTCCTGA TCAATCTACA 
CTAGAGGCAA AGCAGTCCGA GGTCTATGGC ATCACAAACT TTGCGGAAGA ATTGGCAGAG 
ACTGTTGTCT CCATGGCAAC TGAAATCGCA GCAATCTGCC TTGACAACTC CCATGGAAAA 
CAGCCGTGGT TTTGTGCTTG GAAGAGAGGG AACGAATTTC TGATGACGCC TAACGCATCC 
TGCCGATCCT TGAAGAGGAA GAAGGAAAAC TCGGGCACGG GAAGCACTGT GAGGAAACAC 
AAGCCACCTC GCCTCAGTGA GATCAAGAGA AAAGCAGATG AGCACCCAGA GCTGAAAGAG 
AAGCTGATGA ACAGAGTCAT GGATGAGTCC ATGAACCTCG AAGACATTCC CGATTCCGTC 
AGTACTTTTG CAAATGAAGT GGCAGCCAAG ATCATGAACC TAACAGAATT CTCCACGGTG 
GATGGAGTGT GGCAAACCCA GAGTTGTTCC CGGAATCGGC TTCTGGGTGG TGATAGGTGG 
AACCGGCTGA AGGCCTCGAG CTGTGAAAGT ATTCCGGAGG AGGACTCTGA AGCCAGGGTC 
TTTGTAAACA GCCTGGGTTT AATGAGTACC TTAAGCCAAC CCGTTAGCAG GGCCAGCTCA 
GTCTCCAAAC AGTCCAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAA 
CAGATGGAAA ACGAAGGGAG GGGGTTCGAG TTGCTGCTGG ATTACTACGC TGGCAAAAAT 
GCCAGCAGTA TCCTGAACTC AGCAATCCAG CAGGCATGCC AGAAAAACGA CCACCTCAAC 
GTAAGACCAA GCTGCCCCTC TAAGCAGTCC AGCACAGAAA GCATAACAGA GGAGTTCTAT 
AAGTACATGC TGAGAGACAT TGCCAAAGAA AACAAAGATG GCGCCCTGTC CAGACGAAGC 
AGCCATGATT GGAGCACGGG CTTGCTGTCT CCTTCCGCGC GATCCCCACT GTGTTACAGG 
CAGTCATCTA TGCCTGACAG CAGGTCCCCG TGCTCCAGGT TAACAGTAAA TGCACCTATC 
AAAGCCAACT CCTTAGATGG TTTTGCCCAA AACTGCCCTC AAGATTCTAT AAATGTACAG 
CCAGTCAGTA GGGCTTCCTC GTCTGGCCTC TGCAAATCAG ACTCCTGTTT ATATCGCCGA 
AGTGGGACTG ACCAGATCAC AAACATGCTA ATTCATGAAA CATGGGCCAG CTCCATTGAG 
GCTCTCATGA GAAAGAACAA AATCATTGCT GATGACGGCG AGGCAGCTAA CGCTAGCCCT 
GGTCCTGTTT CTGGCGGCTC GCCTTCGCAA GTAGAGAAGT GTGCCAACAG ACTAGTCACC 
GGCACAGGAC ACAAGGGACC AGCTCTACTT GTACAGGAGT CTGTTGATTA CCAGAGAAAG 
GATGCTGTCA CTGAAGGCAA TTGTTCCCCA GTGTCATCTC CAAGCAAAAT GGCTCCTGTT 
AAAAAGCCCA GTGGTTTTGA TCCTACAAGA GAAACCTCTG CATGCCACAA TGCTGTTGCT 
CTCAAAAGCC CCAGGCGGTC TCTTTGCTCA AGGGAGGTGC CTTTGATTCA GATAGAGACC 
GATCAGAAAG AAGAATGCGT TGGAGAATCT GAAACCTTAC TTCCCCAAAG TGGCTCCCTA 
GAGGAAGCAG AAGGGCCCCA GCCTGAGGAA ACCATCCCAG ATGTGGCCAG AAGCGAAGAC 
ACAGCCCTGA GCGCCTGTCA AAGCTCTCAT GACAGCCTTG AGACCAGAGA AGAATTAGAA 
GTGGATGTCT TGAAAGAAGA CATAACCCTA GATGAGTCCC GGAACCCTCC TAGCAGCAGC 
GAAGAGAGTA CAGGCAGCTG GTCCCAGCTG GCCAATGAGG AGGACAACCC AGATGACACA 
AGTAGCTTTC TGCAGCTCAG TGAGCGGTCC ATGAGCAATG GCAACACTAG TGGCACTAGC 
AGTCTTGGCA TTATGGACCT GGACATTTAT CAGGAAAGCA TACCGTCTTC TCCCATGATT 
AATGAATTAG TAGAAGAAAA GGAGATTCTT AAAGAACAAT CAGAAAGCGT AAAGGAACGT 
GCCTCTGGAC TGCCAGGGAG AGCAGCCAGC CCCCAGAGGA GTCTACTGGT GATCAACTTT 
GACCTGGAGC CAGAGTGTCC TGATGCTGAG CTGCGAGCCA CTCTGCAGTG GATAGCTGCC 
TCCGAGCTGG GCATCCCAAC AATCTACTTT AAGAAATCTC AGGAAAGCAG AATTGAAAAG 
TTTCTAGATG TTGTGAAGCT TGTTCATCAG AAGTCCTGGA AGGTGGGAGA TATATTTCAT 
GCCGTTGTCC AGTACTGCAA AATGCATGCA GAGCAGAAAG AGGGAACTCC GAGTCTCTTT 
GACTGGCTCC TGGAACTAGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( XM_006245231.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245293.1 Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X2, mRNA.

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CloneID	ORa32396
Clone ID Related Accession (Same CDS sequence)	XM_006245231.3
Accession Version	XM_006245231.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245231.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAA AAGCAACCAT GTGGGTAGTT GAACCAAACT CTGCTACCTG CCTTTCTGCT 
GCCCCGCCGG TCAAGAGACA CTGCCAAGAA TCCAGCAACG TGGAATCACC ACTGATGCCC 
GAGGACTCGG AGCCACAGCA GATCACCAGC AGTGCAGCAG GCAGCCTGGC TGGCTCCATC 
ACGGCCTGTA AGAAGGTTCT TCGAAGCAAT AGCTTGCTGG AGTCCACAGA CTACTGGTTG 
CAGAACCAGA GGACGCCTTG CCAGATTGGG TTTGTGGAAG ACGAGTCTGA AAACTGTGCT 
TCTGTCTGCT TTGTGAACCT GGATGTCAAC AAGGATGGCT GCAGCACAGA GAACCTGCAG 
CAGAAACTGG TCAACGTTTC ACCAGACCTT CCAAAACTCA TCAACTCCAT GAATGTCCAG 
AAGCCAAAAG AAAATGAAAT TGTCCTCCTA AGTGGGCTAG CCTCTGGAAA CCGCCAGGCA 
GATTTTGATG TTTCACAGTG CCCTTGGCTA CCAGATATCT GCTTGGTCCA GTGCGCAAGA 
GGGAACAGAC CAAACAGTAC CAACTGCATC ATCTTTGAGA TCAACAAGTT CTTAATTGGT 
CTGGAAGTGG TGCAGGAGCG ACAGCTCCAC CTGGAGACCA GCGTGTTGAA GCTGGAGGAT 
GATACAAACT GTTCCCTGTC TTCGATTGAA GAAGATTTCC TTACAGCCTC AGAGCATTTG 
GAGGAAGAAA TCGAGGTGGA AGACTGCAAA AGTGGTTTGG AAACTATAAA TGTGTCAGCT 
AACGTTCTGG AAAGCAAAAG GCCAAAGGAA GCTACCCAGG AAGGATGGGA TTACCACAAG 
GAAATATTGC ATTGTGCTTT AGGTGAAAAA CACATCAGAA AACACCGTAT GCCCTCAATG 
AAAACAGAAA GGTCAAAGGA AAACACAGCA GAGAACACAG CCCTGCAAAG TCTCAATCCT 
TCAGCCAGAC CATCACGCTT GAAAAGTGAA GTGGCGGGGA GCAAGCAGCC AGCCACGAAC 
TACTCTTACC CCGAAAATAT CAGAGGAGAA ATAGAAACAT CTCAGATGCT GTTCATCCCC 
AGGGATGCCT ATCTCTCCAT GGTTTCTTCA TGTGGTGTAC TCACTGAGCA GGGAAGCAAC 
CACAGAGACC ATGACGCCAC TCCAAACTCC CTTCCTCCCG TTCAAAATGG AGAAGCCACC 
GCGGGCGAGT ACGCTACCAA CTTAGCAGAA TCTGTAATGC AAGATGCCTT CATCCGATTG 
TCTCAGTCGC AACCTACACT CCCCCAGGAA TCTGCAGTCA GTTTCTCTGT GGGAAGCGCT 
CTGCTCCCCA GTGGTTGTTG CACCAAAGAT ATGGTGGTCC CTCGTTCATG GAATGAGCTT 
CCTAAAATTG TCATCGTGCA GAGCCCAGAG GGCAGCGACA CAGCCCCTGA GCCAAGTGTC 
TCCTCCTGGC CCGACCTGGA AGTGTCTGTC GAGACCCCGG GCATCTTTTC TGAAGAGAGC 
TCCAGCAGAC CCACTCAGAG TGCCCTAGAA GTTGCTTTGG CTTGTGCAGC CACTGTGATT 
GGAACCACTT CCAGTCCACA GGCCACAGAA AGATTCACCA TGGAGCAAGA ATCTCTGGTA 
TCTACCTACA CCCAGAGAGG CAATGGGATA CAGCAAACTC CAGTGCCCAG AGTATTCATG 
GGACCTTCTA CAACTGAATA CTCCTTCCCA TCAGCTCTGT GTGGCATGAC CCAAGTGGCA 
AGCGCTGTTG CTGTCTGTGG CCTAGGGGAA AGAGAAGAGG TGACATATTC TGTAGCTCCA 
AGTGACCTCC TGCCCACACC TGGGGCTTCT GAAGAAAGAT CCTCCATTGG AAGTTTAGTG 
ACAGAGGAAA GCACAGAACT GGGGAAGGAA GCCATTGCAG AGGCTCTGCT CAGGGAGGCT 
ACTCTGGTTT TAGCGAGGCC GAATGCATAC AGCAGCGTCG GAGAGCTCTT GGAATCTGTG 
AACCGGAGGA TCATAGAAAC CACTTCTAAA ACCCAGATGC TGTGCACAGA AAATGTTCGA 
AGGAATGAAC TGGCACACAC CTTGTCCAAT GTCATCCTCA AGCATTCCAT TGATGAGCTG 
CACCAGAAAA ATATCATGGC TCACCCCACC GAGGAAAGGC ATCCCGGTGG GACTCTGAAC 
ACCTTAATGG AAAGTGTGAA CCAGTTGCTG CACAACGTGA TGTGCTTCAC CTTCAGAAAG 
ATGAATCACA TCGTAACACT GGGTGAGCAT CCCGCTGGCC TTTCTAAGGA GACTGTCACT 
TGGACAGAGT CAGGGCCCTT AGGTTGCCAG TCATTTGATA AGGCTGCTGG TCATGCATGG 
GTTAAAGCCT CGGCTTGCTC CAGCAGCCAT CCCTCTAGCA ATGCACACGG TACTGGCCTT 
GTCATCAGAG ATCTTGTAGA AGATGCATCT CCTAAGCCAG ACAAGGGAGG AGCAAGGCCA 
GAACTGGTCA ACAACCCCAG GCTGCAATCT GAATTCTCAT GCAGTCACAG AATGCTTGAT 
TCAACTGCTA GGACATTCCC CAAGGAAATG TATCCAAAAG GGATTGTGGG AGAGGATACA 
AGAAACCCTC TTCATACACT AAGTTATGAC AGCAGTGAAC AAAGAACTTC TACAGATATA 
GGAAAATTAA CAACAGTAGG AGAGGTCCGT AGTACCTTCC AGGAATCTGA AGACAGCATT 
GTTCCAAAAG CCCAAGAGAA ACACACTTGT GCCACAACTC TAAACAACGA AGCTCAAATT 
AACCTATCCT TGTTAGGGGA TGACCTAGTG GTTCCTGATC AATCTACACT AGAGGCAAAG 
CAGTCCGAGG TCTATGGCAT CACAAACTTT GCGGAAGAAT TGGCAGAGAC TGTTGTCTCC 
ATGGCAACTG AAATCGCAGC AATCTGCCTT GACAACTCCC ATGGAAAACA GCCGTGGTTT 
TGTGCTTGGA AGAGAGGGAA CGAATTTCTG ATGACGCCTA ACGCATCCTG CCGATCCTTG 
AAGAGGAAGA AGGAAAACTC GGGCACGGGA AGCACTGTGA GGAAACACAA GCCACCTCGC 
CTCAGTGAGA TCAAGAGAAA AGCAGATGAG CACCCAGAGC TGAAAGAGAA GCTGATGAAC 
AGAGTCATGG ATGAGTCCAT GAACCTCGAA GACATTCCCG ATTCCGTCAG TACTTTTGCA 
AATGAAGTGG CAGCCAAGAT CATGAACCTA ACAGAATTCT CCACGGTGGA TGGAGTGTGG 
CAAACCCAGA GTTGTTCCCG GAATCGGCTT CTGGGTGGTG ATAGGTGGAA CCGGCTGAAG 
GCCTCGAGCT GTGAAAGTAT TCCGGAGGAG GACTCTGAAG CCAGGGTCTT TGTAAACAGC 
CTGGGTTTAA TGAGTACCTT AAGCCAACCC GTTAGCAGGG CCAGCTCAGT CTCCAAACAG 
TCCAGCTGTG AGAGCATCAC CGATGAGTTT TCCAGGTTCA TGGTGAAACA GATGGAAAAC 
GAAGGGAGGG GGTTCGAGTT GCTGCTGGAT TACTACGCTG GCAAAAATGC CAGCAGTATC 
CTGAACTCAG CAATCCAGCA GGCATGCCAG AAAAACGACC ACCTCAACGT AAGACCAAGC 
TGCCCCTCTA AGCAGTCCAG CACAGAAAGC ATAACAGAGG AGTTCTATAA GTACATGCTG 
AGAGACATTG CCAAAGAAAA CAAAGATGGC GCCCTGTCCA GACGAAGCAG CCATGATTGG 
AGCACGGGCT TGCTGTCTCC TTCCGCGCGA TCCCCACTGT GTTACAGGCA GTCATCTATG 
CCTGACAGCA GGTCCCCGTG CTCCAGGTTA ACAGTAAATG CACCTATCAA AGCCAACTCC 
TTAGATGGTT TTGCCCAAAA CTGCCCTCAA GATTCTATAA ATGTACAGCC AGTCAGTAGG 
GCTTCCTCGT CTGGCCTCTG CAAATCAGAC TCCTGTTTAT ATCGCCGAAG TGGGACTGAC 
CAGATCACAA ACATGCTAAT TCATGAAACA TGGGCCAGCT CCATTGAGGC TCTCATGAGA 
AAGAACAAAA TCATTGCTGA TGACGGCGAG GCAGCTAACG CTAGCCCTGG TCCTGTTTCT 
GGCGGCTCGC CTTCGCAAGT AGAGAAGTGT GCCAACAGAC TAGTCACCGG CACAGGACAC 
AAGGGACCAG CTCTACTTGT ACAGGAGTCT GTTGATTACC AGAGAAAGGA TGCTGTCACT 
GAAGGCAATT GTTCCCCAGT GTCATCTCCA AGCAAAATGG CTCCTGTTAA AAAGCCCAGT 
GGTTTTGATC CTACAAGAGA AACCTCTGCA TGCCACAATG CTGTTGCTCT CAAAAGCCCC 
AGGCGGTCTC TTTGCTCAAG GGAGGTGCCT TTGATTCAGA TAGAGACCGA TCAGAAAGAA 
GAATGCGTTG GAGAATCTGA AACCTTACTT CCCCAAAGTG GCTCCCTAGA GGAAGCAGAA 
GGGCCCCAGC CTGAGGAAAC CATCCCAGAT GTGGCCAGAA GCGAAGACAC AGCCCTGAGC 
GCCTGTCAAA GCTCTCATGA CAGCCTTGAG ACCAGAGAAG AATTAGAAGT GGATGTCTTG 
AAAGAAGACA TAACCCTAGA TGAGTCCCGG AACCCTCCTA GCAGCAGCGA AGAGAGTACA 
GGCAGCTGGT CCCAGCTGGC CAATGAGGAG GACAACCCAG ATGACACAAG TAGCTTTCTG 
CAGCTCAGTG AGCGGTCCAT GAGCAATGGC AACACTAGTG GCACTAGCAG TCTTGGCATT 
ATGGACCTGG ACATTTATCA GGAAAGCATA CCGTCTTCTC CCATGATTAA TGAATTAGTA 
GAAGAAAAGG AGATTCTTAA AGAACAATCA GAAAGCGTAA AGGAACGTGC CTCTGGACTG 
CCAGGGAGAG CAGCCAGCCC CCAGAGGAGT CTACTGGTGA TCAACTTTGA CCTGGAGCCA 
GAGTGTCCTG ATGCTGAGCT GCGAGCCACT CTGCAGTGGA TAGCTGCCTC CGAGCTGGGC 
ATCCCAACAA TCTACTTTAA GAAATCTCAG GAAAGCAGAA TTGAAAAGTT TCTAGATGTT 
GTGAAGCTTG TTCATCAGAA GTCCTGGAAG GTGGGAGATA TATTTCATGC CGTTGTCCAG 
TACTGCAAAA TGCATGCAGA GCAGAAAGAG GGAACTCCGA GTCTCTTTGA CTGGCTCCTG 
GAACTAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245293.1
CDS	727..5898
Translation	MSGKATMWVVEPNSATCLSAAPPVKRHCQESSNVESPLMPEDSEPQQITSSAAGSLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDGCSTENLQQKLVNVSPDLPKLINSMNVQKPKENEIVLLSGLASGNRQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETSVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVEDCKSGLETINVSANVLESKRPKEATQEGWDYHKEILHCALGEKHIRKHRMPSMKTERSKENTAENTALQSLNPSARPSRLKSEVAGSKQPATNYSYPENIRGEIETSQMLFIPRDAYLSMVSSCGVLTEQGSNHRDHDATPNSLPPVQNGEATAGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSVGSALLPSGCCTKDMVVPRSWNELPKIVIVQSPEGSDTAPEPSVSSWPDLEVSVETPGIFSEESSSRPTQSALEVALACAATVIGTTSSPQATERFTMEQESLVSTYTQRGNGIQQTPVPRVFMGPSTTEYSFPSALCGMTQVASAVAVCGLGEREEVTYSVAPSDLLPTPGASEERSSIGSLVTEESTELGKEAIAEALLREATLVLARPNAYSSVGELLESVNRRIIETTSKTQMLCTENVRRNELAHTLSNVILKHSIDELHQKNIMAHPTEERHPGGTLNTLMESVNQLLHNVMCFTFRKMNHIVTLGEHPAGLSKETVTWTESGPLGCQSFDKAAGHAWVKASACSSSHPSSNAHGTGLVIRDLVEDASPKPDKGGARPELVNNPRLQSEFSCSHRMLDSTARTFPKEMYPKGIVGEDTRNPLHTLSYDSSEQRTSTDIGKLTTVGEVRSTFQESEDSIVPKAQEKHTCATTLNNEAQINLSLLGDDLVVPDQSTLEAKQSEVYGITNFAEELAETVVSMATEIAAICLDNSHGKQPWFCAWKRGNEFLMTPNASCRSLKRKKENSGTGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSTVDGVWQTQSCSRNRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSILNSAIQQACQKNDHLNVRPSCPSKQSSTESITEEFYKYMLRDIAKENKDGALSRRSSHDWSTGLLSPSARSPLCYRQSSMPDSRSPCSRLTVNAPIKANSLDGFAQNCPQDSINVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDGEAANASPGPVSGGSPSQVEKCANRLVTGTGHKGPALLVQESVDYQRKDAVTEGNCSPVSSPSKMAPVKKPSGFDPTRETSACHNAVALKSPRRSLCSREVPLIQIETDQKEECVGESETLLPQSGSLEEAEGPQPEETIPDVARSEDTALSACQSSHDSLETREELEVDVLKEDITLDESRNPPSSSEESTGSWSQLANEEDNPDDTSSFLQLSERSMSNGNTSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESVKERASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVHQKSWKVGDIFHAVVQYCKMHAEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006245231.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245231.3

ORF Insert Sequence:

ATGTCTGGAA AAGCAACCAT GTGGGTAGTT GAACCAAACT CTGCTACCTG CCTTTCTGCT 
GCCCCGCCGG TCAAGAGACA CTGCCAAGAA TCCAGCAACG TGGAATCACC ACTGATGCCC 
GAGGACTCGG AGCCACAGCA GATCACCAGC AGTGCAGCAG GCAGCCTGGC TGGCTCCATC 
ACGGCCTGTA AGAAGGTTCT TCGAAGCAAT AGCTTGCTGG AGTCCACAGA CTACTGGTTG 
CAGAACCAGA GGACGCCTTG CCAGATTGGG TTTGTGGAAG ACGAGTCTGA AAACTGTGCT 
TCTGTCTGCT TTGTGAACCT GGATGTCAAC AAGGATGGCT GCAGCACAGA GAACCTGCAG 
CAGAAACTGG TCAACGTTTC ACCAGACCTT CCAAAACTCA TCAACTCCAT GAATGTCCAG 
AAGCCAAAAG AAAATGAAAT TGTCCTCCTA AGTGGGCTAG CCTCTGGAAA CCGCCAGGCA 
GATTTTGATG TTTCACAGTG CCCTTGGCTA CCAGATATCT GCTTGGTCCA GTGCGCAAGA 
GGGAACAGAC CAAACAGTAC CAACTGCATC ATCTTTGAGA TCAACAAGTT CTTAATTGGT 
CTGGAAGTGG TGCAGGAGCG ACAGCTCCAC CTGGAGACCA GCGTGTTGAA GCTGGAGGAT 
GATACAAACT GTTCCCTGTC TTCGATTGAA GAAGATTTCC TTACAGCCTC AGAGCATTTG 
GAGGAAGAAA TCGAGGTGGA AGACTGCAAA AGTGGTTTGG AAACTATAAA TGTGTCAGCT 
AACGTTCTGG AAAGCAAAAG GCCAAAGGAA GCTACCCAGG AAGGATGGGA TTACCACAAG 
GAAATATTGC ATTGTGCTTT AGGTGAAAAA CACATCAGAA AACACCGTAT GCCCTCAATG 
AAAACAGAAA GGTCAAAGGA AAACACAGCA GAGAACACAG CCCTGCAAAG TCTCAATCCT 
TCAGCCAGAC CATCACGCTT GAAAAGTGAA GTGGCGGGGA GCAAGCAGCC AGCCACGAAC 
TACTCTTACC CCGAAAATAT CAGAGGAGAA ATAGAAACAT CTCAGATGCT GTTCATCCCC 
AGGGATGCCT ATCTCTCCAT GGTTTCTTCA TGTGGTGTAC TCACTGAGCA GGGAAGCAAC 
CACAGAGACC ATGACGCCAC TCCAAACTCC CTTCCTCCCG TTCAAAATGG AGAAGCCACC 
GCGGGCGAGT ACGCTACCAA CTTAGCAGAA TCTGTAATGC AAGATGCCTT CATCCGATTG 
TCTCAGTCGC AACCTACACT CCCCCAGGAA TCTGCAGTCA GTTTCTCTGT GGGAAGCGCT 
CTGCTCCCCA GTGGTTGTTG CACCAAAGAT ATGGTGGTCC CTCGTTCATG GAATGAGCTT 
CCTAAAATTG TCATCGTGCA GAGCCCAGAG GGCAGCGACA CAGCCCCTGA GCCAAGTGTC 
TCCTCCTGGC CCGACCTGGA AGTGTCTGTC GAGACCCCGG GCATCTTTTC TGAAGAGAGC 
TCCAGCAGAC CCACTCAGAG TGCCCTAGAA GTTGCTTTGG CTTGTGCAGC CACTGTGATT 
GGAACCACTT CCAGTCCACA GGCCACAGAA AGATTCACCA TGGAGCAAGA ATCTCTGGTA 
TCTACCTACA CCCAGAGAGG CAATGGGATA CAGCAAACTC CAGTGCCCAG AGTATTCATG 
GGACCTTCTA CAACTGAATA CTCCTTCCCA TCAGCTCTGT GTGGCATGAC CCAAGTGGCA 
AGCGCTGTTG CTGTCTGTGG CCTAGGGGAA AGAGAAGAGG TGACATATTC TGTAGCTCCA 
AGTGACCTCC TGCCCACACC TGGGGCTTCT GAAGAAAGAT CCTCCATTGG AAGTTTAGTG 
ACAGAGGAAA GCACAGAACT GGGGAAGGAA GCCATTGCAG AGGCTCTGCT CAGGGAGGCT 
ACTCTGGTTT TAGCGAGGCC GAATGCATAC AGCAGCGTCG GAGAGCTCTT GGAATCTGTG 
AACCGGAGGA TCATAGAAAC CACTTCTAAA ACCCAGATGC TGTGCACAGA AAATGTTCGA 
AGGAATGAAC TGGCACACAC CTTGTCCAAT GTCATCCTCA AGCATTCCAT TGATGAGCTG 
CACCAGAAAA ATATCATGGC TCACCCCACC GAGGAAAGGC ATCCCGGTGG GACTCTGAAC 
ACCTTAATGG AAAGTGTGAA CCAGTTGCTG CACAACGTGA TGTGCTTCAC CTTCAGAAAG 
ATGAATCACA TCGTAACACT GGGTGAGCAT CCCGCTGGCC TTTCTAAGGA GACTGTCACT 
TGGACAGAGT CAGGGCCCTT AGGTTGCCAG TCATTTGATA AGGCTGCTGG TCATGCATGG 
GTTAAAGCCT CGGCTTGCTC CAGCAGCCAT CCCTCTAGCA ATGCACACGG TACTGGCCTT 
GTCATCAGAG ATCTTGTAGA AGATGCATCT CCTAAGCCAG ACAAGGGAGG AGCAAGGCCA 
GAACTGGTCA ACAACCCCAG GCTGCAATCT GAATTCTCAT GCAGTCACAG AATGCTTGAT 
TCAACTGCTA GGACATTCCC CAAGGAAATG TATCCAAAAG GGATTGTGGG AGAGGATACA 
AGAAACCCTC TTCATACACT AAGTTATGAC AGCAGTGAAC AAAGAACTTC TACAGATATA 
GGAAAATTAA CAACAGTAGG AGAGGTCCGT AGTACCTTCC AGGAATCTGA AGACAGCATT 
GTTCCAAAAG CCCAAGAGAA ACACACTTGT GCCACAACTC TAAACAACGA AGCTCAAATT 
AACCTATCCT TGTTAGGGGA TGACCTAGTG GTTCCTGATC AATCTACACT AGAGGCAAAG 
CAGTCCGAGG TCTATGGCAT CACAAACTTT GCGGAAGAAT TGGCAGAGAC TGTTGTCTCC 
ATGGCAACTG AAATCGCAGC AATCTGCCTT GACAACTCCC ATGGAAAACA GCCGTGGTTT 
TGTGCTTGGA AGAGAGGGAA CGAATTTCTG ATGACGCCTA ACGCATCCTG CCGATCCTTG 
AAGAGGAAGA AGGAAAACTC GGGCACGGGA AGCACTGTGA GGAAACACAA GCCACCTCGC 
CTCAGTGAGA TCAAGAGAAA AGCAGATGAG CACCCAGAGC TGAAAGAGAA GCTGATGAAC 
AGAGTCATGG ATGAGTCCAT GAACCTCGAA GACATTCCCG ATTCCGTCAG TACTTTTGCA 
AATGAAGTGG CAGCCAAGAT CATGAACCTA ACAGAATTCT CCACGGTGGA TGGAGTGTGG 
CAAACCCAGA GTTGTTCCCG GAATCGGCTT CTGGGTGGTG ATAGGTGGAA CCGGCTGAAG 
GCCTCGAGCT GTGAAAGTAT TCCGGAGGAG GACTCTGAAG CCAGGGTCTT TGTAAACAGC 
CTGGGTTTAA TGAGTACCTT AAGCCAACCC GTTAGCAGGG CCAGCTCAGT CTCCAAACAG 
TCCAGCTGTG AGAGCATCAC CGATGAGTTT TCCAGGTTCA TGGTGAAACA GATGGAAAAC 
GAAGGGAGGG GGTTCGAGTT GCTGCTGGAT TACTACGCTG GCAAAAATGC CAGCAGTATC 
CTGAACTCAG CAATCCAGCA GGCATGCCAG AAAAACGACC ACCTCAACGT AAGACCAAGC 
TGCCCCTCTA AGCAGTCCAG CACAGAAAGC ATAACAGAGG AGTTCTATAA GTACATGCTG 
AGAGACATTG CCAAAGAAAA CAAAGATGGC GCCCTGTCCA GACGAAGCAG CCATGATTGG 
AGCACGGGCT TGCTGTCTCC TTCCGCGCGA TCCCCACTGT GTTACAGGCA GTCATCTATG 
CCTGACAGCA GGTCCCCGTG CTCCAGGTTA ACAGTAAATG CACCTATCAA AGCCAACTCC 
TTAGATGGTT TTGCCCAAAA CTGCCCTCAA GATTCTATAA ATGTACAGCC AGTCAGTAGG 
GCTTCCTCGT CTGGCCTCTG CAAATCAGAC TCCTGTTTAT ATCGCCGAAG TGGGACTGAC 
CAGATCACAA ACATGCTAAT TCATGAAACA TGGGCCAGCT CCATTGAGGC TCTCATGAGA 
AAGAACAAAA TCATTGCTGA TGACGGCGAG GCAGCTAACG CTAGCCCTGG TCCTGTTTCT 
GGCGGCTCGC CTTCGCAAGT AGAGAAGTGT GCCAACAGAC TAGTCACCGG CACAGGACAC 
AAGGGACCAG CTCTACTTGT ACAGGAGTCT GTTGATTACC AGAGAAAGGA TGCTGTCACT 
GAAGGCAATT GTTCCCCAGT GTCATCTCCA AGCAAAATGG CTCCTGTTAA AAAGCCCAGT 
GGTTTTGATC CTACAAGAGA AACCTCTGCA TGCCACAATG CTGTTGCTCT CAAAAGCCCC 
AGGCGGTCTC TTTGCTCAAG GGAGGTGCCT TTGATTCAGA TAGAGACCGA TCAGAAAGAA 
GAATGCGTTG GAGAATCTGA AACCTTACTT CCCCAAAGTG GCTCCCTAGA GGAAGCAGAA 
GGGCCCCAGC CTGAGGAAAC CATCCCAGAT GTGGCCAGAA GCGAAGACAC AGCCCTGAGC 
GCCTGTCAAA GCTCTCATGA CAGCCTTGAG ACCAGAGAAG AATTAGAAGT GGATGTCTTG 
AAAGAAGACA TAACCCTAGA TGAGTCCCGG AACCCTCCTA GCAGCAGCGA AGAGAGTACA 
GGCAGCTGGT CCCAGCTGGC CAATGAGGAG GACAACCCAG ATGACACAAG TAGCTTTCTG 
CAGCTCAGTG AGCGGTCCAT GAGCAATGGC AACACTAGTG GCACTAGCAG TCTTGGCATT 
ATGGACCTGG ACATTTATCA GGAAAGCATA CCGTCTTCTC CCATGATTAA TGAATTAGTA 
GAAGAAAAGG AGATTCTTAA AGAACAATCA GAAAGCGTAA AGGAACGTGC CTCTGGACTG 
CCAGGGAGAG CAGCCAGCCC CCAGAGGAGT CTACTGGTGA TCAACTTTGA CCTGGAGCCA 
GAGTGTCCTG ATGCTGAGCT GCGAGCCACT CTGCAGTGGA TAGCTGCCTC CGAGCTGGGC 
ATCCCAACAA TCTACTTTAA GAAATCTCAG GAAAGCAGAA TTGAAAAGTT TCTAGATGTT 
GTGAAGCTTG TTCATCAGAA GTCCTGGAAG GTGGGAGATA TATTTCATGC CGTTGTCCAG 
TACTGCAAAA TGCATGCAGA GCAGAAAGAG GGAACTCCGA GTCTCTTTGA CTGGCTCCTG 
GAACTAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( XM_006245233.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245295.1 Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X6, mRNA.

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CloneID	ORa32398
Clone ID Related Accession (Same CDS sequence)	XM_006245233.3
Accession Version	XM_006245233.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein SPHKAP isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245233.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTCA ACTCCCGGCT TTCTGTGCAA AGCAACGTGG AATCACCACT GATGCCCGAG 
GACTCGGAGC CACAGCAGAT CACCAGCAGT GCAGCAGGCA GCCTGGCTGG CTCCATCACG 
GCCTGTAAGA AGGTTCTTCG AAGCAATAGC TTGCTGGAGT CCACAGACTA CTGGTTGCAG 
AACCAGAGGA CGCCTTGCCA GATTGGGTTT GTGGAAGACG AGTCTGAAAA CTGTGCTTCT 
GTCTGCTTTG TGAACCTGGA TGTCAACAAG GATGGCTGCA GCACAGAGAA CCTGCAGCAG 
AAACTGGTCA ACGTTTCACC AGACCTTCCA AAACTCATCA ACTCCATGAA TGTCCAGAAG 
CCAAAAGAAA ATGAAATTGT CCTCCTAAGT GGGCTAGCCT CTGGAAACCG CCAGGCAGAT 
TTTGATGTTT CACAGTGCCC TTGGCTACCA GATATCTGCT TGGTCCAGTG CGCAAGAGGG 
AACAGACCAA ACAGTACCAA CTGCATCATC TTTGAGATCA ACAAGTTCTT AATTGGTCTG 
GAAGTGGTGC AGGAGCGACA GCTCCACCTG GAGACCAGCG TGTTGAAGCT GGAGGATGAT 
ACAAACTGTT CCCTGTCTTC GATTGAAGAA GATTTCCTTA CAGCCTCAGA GCATTTGGAG 
GAAGAAATCG AGGTGGAAGA CTGCAAAAGT GGTTTGGAAA CTATAAATGT GTCAGCTAAC 
GTTCTGGAAA GCAAAAGGCC AAAGGAAGCT ACCCAGGAAG GATGGGATTA CCACAAGGAA 
ATATTGCATT GTGCTTTAGG TGAAAAACAC ATCAGAAAAC ACCGTATGCC CTCAATGAAA 
ACAGAAAGGT CAAAGGAAAA CACAGCAGAG AACACAGCCC TGCAAAGTCT CAATCCTTCA 
GCCAGACCAT CACGCTTGAA AAGTGAAGTG GCGGGGAGCA AGCAGCCAGC CACGAACTAC 
TCTTACCCCG AAAATATCAG AGGAGAAATA GAAACATCTC AGATGCTGTT CATCCCCAGG 
GATGCCTATC TCTCCATGGT TTCTTCATGT GGTGTACTCA CTGAGCAGGG AAGCAACCAC 
AGAGACCATG ACGCCACTCC AAACTCCCTT CCTCCCGTTC AAAATGGAGA AGCCACCGCG 
GGCGAGTACG CTACCAACTT AGCAGAATCT GTAATGCAAG ATGCCTTCAT CCGATTGTCT 
CAGTCGCAAC CTACACTCCC CCAGGAATCT GCAGTCAGTT TCTCTGTGGG AAGCGCTCTG 
CTCCCCAGTG GTTGTTGCAC CAAAGATATG GTGGTCCCTC GTTCATGGAA TGAGCTTCCT 
AAAATTGTCA TCGTGCAGAG CCCAGAGGGC AGCGACACAG CCCCTGAGCC AAGTGTCTCC 
TCCTGGCCCG ACCTGGAAGT GTCTGTCGAG ACCCCGGGCA TCTTTTCTGA AGAGAGCTCC 
AGCAGACCCA CTCAGAGTGC CCTAGAAGTT GCTTTGGCTT GTGCAGCCAC TGTGATTGGA 
ACCACTTCCA GTCCACAGGC CACAGAAAGA TTCACCATGG AGCAAGAATC TCTGGTATCT 
ACCTACACCC AGAGAGGCAA TGGGATACAG CAAACTCCAG TGCCCAGAGT ATTCATGGGA 
CCTTCTACAA CTGAATACTC CTTCCCATCA GCTCTGTGTG GCATGACCCA AGTGGCAAGC 
GCTGTTGCTG TCTGTGGCCT AGGGGAAAGA GAAGAGGTGA CATATTCTGT AGCTCCAAGT 
GACCTCCTGC CCACACCTGG GGCTTCTGAA GAAAGATCCT CCATTGGAAG TTTAGTGACA 
GAGGAAAGCA CAGAACTGGG GAAGGAAGCC ATTGCAGAGG CTCTGCTCAG GGAGGCTACT 
CTGGTTTTAG CGAGGCCGAA TGCATACAGC AGCGTCGGAG AGCTCTTGGA ATCTGTGAAC 
CGGAGGATCA TAGAAACCAC TTCTAAAACC CAGATGCTGT GCACAGAAAA TGTTCGAAGG 
AATGAACTGG CACACACCTT GTCCAATGTC ATCCTCAAGC ATTCCATTGA TGAGCTGCAC 
CAGAAAAATA TCATGGCTCA CCCCACCGAG GAAAGGCATC CCGGTGGGAC TCTGAACACC 
TTAATGGAAA GTGTGAACCA GTTGCTGCAC AACGTGATGT GCTTCACCTT CAGAAAGATG 
AATCACATCG TAACACTGGG TGAGCATCCC GCTGGCCTTT CTAAGGAGAC TGTCACTTGG 
ACAGAGTCAG GGCCCTTAGG TTGCCAGTCA TTTGATAAGG CTGCTGGTCA TGCATGGGTT 
AAAGCCTCGG CTTGCTCCAG CAGCCATCCC TCTAGCAATG CACACGGTAC TGGCCTTGTC 
ATCAGAGATC TTGTAGAAGA TGCATCTCCT AAGCCAGACA AGGGAGGAGC AAGGCCAGAA 
CTGGTCAACA ACCCCAGGCT GCAATCTGAA TTCTCATGCA GTCACAGAAT GCTTGATTCA 
ACTGCTAGGA CATTCCCCAA GGAAATGTAT CCAAAAGGGA TTGTGGGAGA GGATACAAGA 
AACCCTCTTC ATACACTAAG TTATGACAGC AGTGAACAAA GAACTTCTAC AGATATAGGA 
AAATTAACAA CAGTAGGAGA GGTCCGTAGT ACCTTCCAGG AATCTGAAGA CAGCATTGTT 
CCAAAAGCCC AAGAGAAACA CACTTGTGCC ACAACTCTAA ACAACGAAGC TCAAATTAAC 
CTATCCTTGT TAGGGGATGA CCTAGTGGTT CCTGATCAAT CTACACTAGA GGCAAAGCAG 
TCCGAGGTCT ATGGCATCAC AAACTTTGCG GAAGAATTGG CAGAGACTGT TGTCTCCATG 
GCAACTGAAA TCGCAGCAAT CTGCCTTGAC AACTCCCATG GAAAACAGCC GTGGTTTTGT 
GCTTGGAAGA GAGGGAACGA ATTTCTGATG ACGCCTAACG CATCCTGCCG ATCCTTGAAG 
AGGAAGAAGG AAAACTCGGG CACGGGAAGC ACTGTGAGGA AACACAAGCC ACCTCGCCTC 
AGTGAGATCA AGAGAAAAGC AGATGAGCAC CCAGAGCTGA AAGAGAAGCT GATGAACAGA 
GTCATGGATG AGTCCATGAA CCTCGAAGAC ATTCCCGATT CCGTCAGTAC TTTTGCAAAT 
GAAGTGGCAG CCAAGATCAT GAACCTAACA GAATTCTCCA CGGTGGATGG AGTGTGGCAA 
ACCCAGAGTT GTTCCCGGAA TCGGCTTCTG GGTGGTGATA GGTGGAACCG GCTGAAGGCC 
TCGAGCTGTG AAAGTATTCC GGAGGAGGAC TCTGAAGCCA GGGTCTTTGT AAACAGCCTG 
GGTTTAATGA GTACCTTAAG CCAACCCGTT AGCAGGGCCA GCTCAGTCTC CAAACAGTCC 
AGCTGTGAGA GCATCACCGA TGAGTTTTCC AGGTTCATGG TGAAACAGAT GGAAAACGAA 
GGGAGGGGGT TCGAGTTGCT GCTGGATTAC TACGCTGGCA AAAATGCCAG CAGTATCCTG 
AACTCAGCAA TCCAGCAGGC ATGCCAGAAA AACGACCACC TCAACGTAAG ACCAAGCTGC 
CCCTCTAAGC AGTCCAGCAC AGAAAGCATA ACAGAGGAGT TCTATAAGTA CATGCTGAGA 
GACATTGCCA AAGAAAACAA AGATGGCGCC CTGTCCAGAC GAAGCAGCCA TGATTGGAGC 
ACGGGCTTGC TGTCTCCTTC CGCGCGATCC CCACTGTGTT ACAGGCAGTC ATCTATGCCT 
GACAGCAGGT CCCCGTGCTC CAGGTTAACA GTAAATGCAC CTATCAAAGC CAACTCCTTA 
GATGGTTTTG CCCAAAACTG CCCTCAAGAT TCTATAAATG TACAGCCAGT CAGTAGGGCT 
TCCTCGTCTG GCCTCTGCAA ATCAGACTCC TGTTTATATC GCCGAAGTGG GACTGACCAG 
ATCACAAACA TGCTAATTCA TGAAACATGG GCCAGCTCCA TTGAGGCTCT CATGAGAAAG 
AACAAAATCA TTGCTGATGA CGGCGAGGCA GCTAACGCTA GCCCTGGTCC TGTTTCTGGC 
GGCTCGCCTT CGCAAGTAGA GAAGTGTGCC AACAGACTAG TCACCGGCAC AGGACACAAG 
GGACCAGCTC TACTTGTACA GGAGTCTGTT GATTACCAGA GAAAGGATGC TGTCACTGAA 
GGCAATTGTT CCCCAGTGTC ATCTCCAAGC AAAATGGCTC CTGTTAAAAA GCCCAGTGGT 
TTTGATCCTA CAAGAGAAAC CTCTGCATGC CACAATGCTG TTGCTCTCAA AAGCCCCAGG 
CGGTCTCTTT GCTCAAGGGA GGTGCCTTTG ATTCAGATAG AGACCGATCA GAAAGAAGAA 
TGCGTTGGAG AATCTGAAAC CTTACTTCCC CAAAGTGGCT CCCTAGAGGA AGCAGAAGGG 
CCCCAGCCTG AGGAAACCAT CCCAGATGTG GCCAGAAGCG AAGACACAGC CCTGAGCGCC 
TGTCAAAGCT CTCATGACAG CCTTGAGACC AGAGAAGAAT TAGAAGTGGA TGTCTTGAAA 
GAAGACATAA CCCTAGATGA GTCCCGGAAC CCTCCTAGCA GCAGCGAAGA GAGTACAGGC 
AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC AACCCAGATG ACACAAGTAG CTTTCTGCAG 
CTCAGTGAGC GGTCCATGAG CAATGGCAAC ACTAGTGGCA CTAGCAGTCT TGGCATTATG 
GACCTGGACA TTTATCAGGA AAGCATACCG TCTTCTCCCA TGATTAATGA ATTAGTAGAA 
GAAAAGGAGA TTCTTAAAGA ACAATCAGAA AGCGTAAAGG AACGTGCCTC TGGACTGCCA 
GGGAGAGCAG CCAGCCCCCA GAGGAGTCTA CTGGTGATCA ACTTTGACCT GGAGCCAGAG 
TGTCCTGATG CTGAGCTGCG AGCCACTCTG CAGTGGATAG CTGCCTCCGA GCTGGGCATC 
CCAACAATCT ACTTTAAGAA ATCTCAGGAA AGCAGAATTG AAAAGTTTCT AGATGTTGTG 
AAGCTTGTTC ATCAGAAGTC CTGGAAGGTG GGAGATATAT TTCATGCCGT TGTCCAGTAC 
TGCAAAATGC ATGCAGAGCA GAAAGAGGGA ACTCCGAGTC TCTTTGACTG GCTCCTGGAA 
CTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245295.1
CDS	309..5417
Translation	MDVNSRLSVQSNVESPLMPEDSEPQQITSSAAGSLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDGCSTENLQQKLVNVSPDLPKLINSMNVQKPKENEIVLLSGLASGNRQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETSVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVEDCKSGLETINVSANVLESKRPKEATQEGWDYHKEILHCALGEKHIRKHRMPSMKTERSKENTAENTALQSLNPSARPSRLKSEVAGSKQPATNYSYPENIRGEIETSQMLFIPRDAYLSMVSSCGVLTEQGSNHRDHDATPNSLPPVQNGEATAGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSVGSALLPSGCCTKDMVVPRSWNELPKIVIVQSPEGSDTAPEPSVSSWPDLEVSVETPGIFSEESSSRPTQSALEVALACAATVIGTTSSPQATERFTMEQESLVSTYTQRGNGIQQTPVPRVFMGPSTTEYSFPSALCGMTQVASAVAVCGLGEREEVTYSVAPSDLLPTPGASEERSSIGSLVTEESTELGKEAIAEALLREATLVLARPNAYSSVGELLESVNRRIIETTSKTQMLCTENVRRNELAHTLSNVILKHSIDELHQKNIMAHPTEERHPGGTLNTLMESVNQLLHNVMCFTFRKMNHIVTLGEHPAGLSKETVTWTESGPLGCQSFDKAAGHAWVKASACSSSHPSSNAHGTGLVIRDLVEDASPKPDKGGARPELVNNPRLQSEFSCSHRMLDSTARTFPKEMYPKGIVGEDTRNPLHTLSYDSSEQRTSTDIGKLTTVGEVRSTFQESEDSIVPKAQEKHTCATTLNNEAQINLSLLGDDLVVPDQSTLEAKQSEVYGITNFAEELAETVVSMATEIAAICLDNSHGKQPWFCAWKRGNEFLMTPNASCRSLKRKKENSGTGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSTVDGVWQTQSCSRNRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSILNSAIQQACQKNDHLNVRPSCPSKQSSTESITEEFYKYMLRDIAKENKDGALSRRSSHDWSTGLLSPSARSPLCYRQSSMPDSRSPCSRLTVNAPIKANSLDGFAQNCPQDSINVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDGEAANASPGPVSGGSPSQVEKCANRLVTGTGHKGPALLVQESVDYQRKDAVTEGNCSPVSSPSKMAPVKKPSGFDPTRETSACHNAVALKSPRRSLCSREVPLIQIETDQKEECVGESETLLPQSGSLEEAEGPQPEETIPDVARSEDTALSACQSSHDSLETREELEVDVLKEDITLDESRNPPSSSEESTGSWSQLANEEDNPDDTSSFLQLSERSMSNGNTSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESVKERASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVHQKSWKVGDIFHAVVQYCKMHAEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006245233.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245233.3

ORF Insert Sequence:

ATGGATGTCA ACTCCCGGCT TTCTGTGCAA AGCAACGTGG AATCACCACT GATGCCCGAG 
GACTCGGAGC CACAGCAGAT CACCAGCAGT GCAGCAGGCA GCCTGGCTGG CTCCATCACG 
GCCTGTAAGA AGGTTCTTCG AAGCAATAGC TTGCTGGAGT CCACAGACTA CTGGTTGCAG 
AACCAGAGGA CGCCTTGCCA GATTGGGTTT GTGGAAGACG AGTCTGAAAA CTGTGCTTCT 
GTCTGCTTTG TGAACCTGGA TGTCAACAAG GATGGCTGCA GCACAGAGAA CCTGCAGCAG 
AAACTGGTCA ACGTTTCACC AGACCTTCCA AAACTCATCA ACTCCATGAA TGTCCAGAAG 
CCAAAAGAAA ATGAAATTGT CCTCCTAAGT GGGCTAGCCT CTGGAAACCG CCAGGCAGAT 
TTTGATGTTT CACAGTGCCC TTGGCTACCA GATATCTGCT TGGTCCAGTG CGCAAGAGGG 
AACAGACCAA ACAGTACCAA CTGCATCATC TTTGAGATCA ACAAGTTCTT AATTGGTCTG 
GAAGTGGTGC AGGAGCGACA GCTCCACCTG GAGACCAGCG TGTTGAAGCT GGAGGATGAT 
ACAAACTGTT CCCTGTCTTC GATTGAAGAA GATTTCCTTA CAGCCTCAGA GCATTTGGAG 
GAAGAAATCG AGGTGGAAGA CTGCAAAAGT GGTTTGGAAA CTATAAATGT GTCAGCTAAC 
GTTCTGGAAA GCAAAAGGCC AAAGGAAGCT ACCCAGGAAG GATGGGATTA CCACAAGGAA 
ATATTGCATT GTGCTTTAGG TGAAAAACAC ATCAGAAAAC ACCGTATGCC CTCAATGAAA 
ACAGAAAGGT CAAAGGAAAA CACAGCAGAG AACACAGCCC TGCAAAGTCT CAATCCTTCA 
GCCAGACCAT CACGCTTGAA AAGTGAAGTG GCGGGGAGCA AGCAGCCAGC CACGAACTAC 
TCTTACCCCG AAAATATCAG AGGAGAAATA GAAACATCTC AGATGCTGTT CATCCCCAGG 
GATGCCTATC TCTCCATGGT TTCTTCATGT GGTGTACTCA CTGAGCAGGG AAGCAACCAC 
AGAGACCATG ACGCCACTCC AAACTCCCTT CCTCCCGTTC AAAATGGAGA AGCCACCGCG 
GGCGAGTACG CTACCAACTT AGCAGAATCT GTAATGCAAG ATGCCTTCAT CCGATTGTCT 
CAGTCGCAAC CTACACTCCC CCAGGAATCT GCAGTCAGTT TCTCTGTGGG AAGCGCTCTG 
CTCCCCAGTG GTTGTTGCAC CAAAGATATG GTGGTCCCTC GTTCATGGAA TGAGCTTCCT 
AAAATTGTCA TCGTGCAGAG CCCAGAGGGC AGCGACACAG CCCCTGAGCC AAGTGTCTCC 
TCCTGGCCCG ACCTGGAAGT GTCTGTCGAG ACCCCGGGCA TCTTTTCTGA AGAGAGCTCC 
AGCAGACCCA CTCAGAGTGC CCTAGAAGTT GCTTTGGCTT GTGCAGCCAC TGTGATTGGA 
ACCACTTCCA GTCCACAGGC CACAGAAAGA TTCACCATGG AGCAAGAATC TCTGGTATCT 
ACCTACACCC AGAGAGGCAA TGGGATACAG CAAACTCCAG TGCCCAGAGT ATTCATGGGA 
CCTTCTACAA CTGAATACTC CTTCCCATCA GCTCTGTGTG GCATGACCCA AGTGGCAAGC 
GCTGTTGCTG TCTGTGGCCT AGGGGAAAGA GAAGAGGTGA CATATTCTGT AGCTCCAAGT 
GACCTCCTGC CCACACCTGG GGCTTCTGAA GAAAGATCCT CCATTGGAAG TTTAGTGACA 
GAGGAAAGCA CAGAACTGGG GAAGGAAGCC ATTGCAGAGG CTCTGCTCAG GGAGGCTACT 
CTGGTTTTAG CGAGGCCGAA TGCATACAGC AGCGTCGGAG AGCTCTTGGA ATCTGTGAAC 
CGGAGGATCA TAGAAACCAC TTCTAAAACC CAGATGCTGT GCACAGAAAA TGTTCGAAGG 
AATGAACTGG CACACACCTT GTCCAATGTC ATCCTCAAGC ATTCCATTGA TGAGCTGCAC 
CAGAAAAATA TCATGGCTCA CCCCACCGAG GAAAGGCATC CCGGTGGGAC TCTGAACACC 
TTAATGGAAA GTGTGAACCA GTTGCTGCAC AACGTGATGT GCTTCACCTT CAGAAAGATG 
AATCACATCG TAACACTGGG TGAGCATCCC GCTGGCCTTT CTAAGGAGAC TGTCACTTGG 
ACAGAGTCAG GGCCCTTAGG TTGCCAGTCA TTTGATAAGG CTGCTGGTCA TGCATGGGTT 
AAAGCCTCGG CTTGCTCCAG CAGCCATCCC TCTAGCAATG CACACGGTAC TGGCCTTGTC 
ATCAGAGATC TTGTAGAAGA TGCATCTCCT AAGCCAGACA AGGGAGGAGC AAGGCCAGAA 
CTGGTCAACA ACCCCAGGCT GCAATCTGAA TTCTCATGCA GTCACAGAAT GCTTGATTCA 
ACTGCTAGGA CATTCCCCAA GGAAATGTAT CCAAAAGGGA TTGTGGGAGA GGATACAAGA 
AACCCTCTTC ATACACTAAG TTATGACAGC AGTGAACAAA GAACTTCTAC AGATATAGGA 
AAATTAACAA CAGTAGGAGA GGTCCGTAGT ACCTTCCAGG AATCTGAAGA CAGCATTGTT 
CCAAAAGCCC AAGAGAAACA CACTTGTGCC ACAACTCTAA ACAACGAAGC TCAAATTAAC 
CTATCCTTGT TAGGGGATGA CCTAGTGGTT CCTGATCAAT CTACACTAGA GGCAAAGCAG 
TCCGAGGTCT ATGGCATCAC AAACTTTGCG GAAGAATTGG CAGAGACTGT TGTCTCCATG 
GCAACTGAAA TCGCAGCAAT CTGCCTTGAC AACTCCCATG GAAAACAGCC GTGGTTTTGT 
GCTTGGAAGA GAGGGAACGA ATTTCTGATG ACGCCTAACG CATCCTGCCG ATCCTTGAAG 
AGGAAGAAGG AAAACTCGGG CACGGGAAGC ACTGTGAGGA AACACAAGCC ACCTCGCCTC 
AGTGAGATCA AGAGAAAAGC AGATGAGCAC CCAGAGCTGA AAGAGAAGCT GATGAACAGA 
GTCATGGATG AGTCCATGAA CCTCGAAGAC ATTCCCGATT CCGTCAGTAC TTTTGCAAAT 
GAAGTGGCAG CCAAGATCAT GAACCTAACA GAATTCTCCA CGGTGGATGG AGTGTGGCAA 
ACCCAGAGTT GTTCCCGGAA TCGGCTTCTG GGTGGTGATA GGTGGAACCG GCTGAAGGCC 
TCGAGCTGTG AAAGTATTCC GGAGGAGGAC TCTGAAGCCA GGGTCTTTGT AAACAGCCTG 
GGTTTAATGA GTACCTTAAG CCAACCCGTT AGCAGGGCCA GCTCAGTCTC CAAACAGTCC 
AGCTGTGAGA GCATCACCGA TGAGTTTTCC AGGTTCATGG TGAAACAGAT GGAAAACGAA 
GGGAGGGGGT TCGAGTTGCT GCTGGATTAC TACGCTGGCA AAAATGCCAG CAGTATCCTG 
AACTCAGCAA TCCAGCAGGC ATGCCAGAAA AACGACCACC TCAACGTAAG ACCAAGCTGC 
CCCTCTAAGC AGTCCAGCAC AGAAAGCATA ACAGAGGAGT TCTATAAGTA CATGCTGAGA 
GACATTGCCA AAGAAAACAA AGATGGCGCC CTGTCCAGAC GAAGCAGCCA TGATTGGAGC 
ACGGGCTTGC TGTCTCCTTC CGCGCGATCC CCACTGTGTT ACAGGCAGTC ATCTATGCCT 
GACAGCAGGT CCCCGTGCTC CAGGTTAACA GTAAATGCAC CTATCAAAGC CAACTCCTTA 
GATGGTTTTG CCCAAAACTG CCCTCAAGAT TCTATAAATG TACAGCCAGT CAGTAGGGCT 
TCCTCGTCTG GCCTCTGCAA ATCAGACTCC TGTTTATATC GCCGAAGTGG GACTGACCAG 
ATCACAAACA TGCTAATTCA TGAAACATGG GCCAGCTCCA TTGAGGCTCT CATGAGAAAG 
AACAAAATCA TTGCTGATGA CGGCGAGGCA GCTAACGCTA GCCCTGGTCC TGTTTCTGGC 
GGCTCGCCTT CGCAAGTAGA GAAGTGTGCC AACAGACTAG TCACCGGCAC AGGACACAAG 
GGACCAGCTC TACTTGTACA GGAGTCTGTT GATTACCAGA GAAAGGATGC TGTCACTGAA 
GGCAATTGTT CCCCAGTGTC ATCTCCAAGC AAAATGGCTC CTGTTAAAAA GCCCAGTGGT 
TTTGATCCTA CAAGAGAAAC CTCTGCATGC CACAATGCTG TTGCTCTCAA AAGCCCCAGG 
CGGTCTCTTT GCTCAAGGGA GGTGCCTTTG ATTCAGATAG AGACCGATCA GAAAGAAGAA 
TGCGTTGGAG AATCTGAAAC CTTACTTCCC CAAAGTGGCT CCCTAGAGGA AGCAGAAGGG 
CCCCAGCCTG AGGAAACCAT CCCAGATGTG GCCAGAAGCG AAGACACAGC CCTGAGCGCC 
TGTCAAAGCT CTCATGACAG CCTTGAGACC AGAGAAGAAT TAGAAGTGGA TGTCTTGAAA 
GAAGACATAA CCCTAGATGA GTCCCGGAAC CCTCCTAGCA GCAGCGAAGA GAGTACAGGC 
AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC AACCCAGATG ACACAAGTAG CTTTCTGCAG 
CTCAGTGAGC GGTCCATGAG CAATGGCAAC ACTAGTGGCA CTAGCAGTCT TGGCATTATG 
GACCTGGACA TTTATCAGGA AAGCATACCG TCTTCTCCCA TGATTAATGA ATTAGTAGAA 
GAAAAGGAGA TTCTTAAAGA ACAATCAGAA AGCGTAAAGG AACGTGCCTC TGGACTGCCA 
GGGAGAGCAG CCAGCCCCCA GAGGAGTCTA CTGGTGATCA ACTTTGACCT GGAGCCAGAG 
TGTCCTGATG CTGAGCTGCG AGCCACTCTG CAGTGGATAG CTGCCTCCGA GCTGGGCATC 
CCAACAATCT ACTTTAAGAA ATCTCAGGAA AGCAGAATTG AAAAGTTTCT AGATGTTGTG 
AAGCTTGTTC ATCAGAAGTC CTGGAAGGTG GGAGATATAT TTCATGCCGT TGTCCAGTAC 
TGCAAAATGC ATGCAGAGCA GAAAGAGGGA ACTCCGAGTC TCTTTGACTG GCTCCTGGAA 
CTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( XM_006245234.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245296.1 Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X7, mRNA.

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CloneID	ORa32399
Clone ID Related Accession (Same CDS sequence)	XM_006245234.3
Accession Version	XM_006245234.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein SPHKAP isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245234.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCCGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC CCGAGGACTC GGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAGCCTG 
GCTGGCTCCA TCACGGCCTG TAAGAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGT TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGACGAGTCT 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAGGATGG CTGCAGCACA 
GAGAACCTGC AGCAGAAACT GGTCAACGTT TCACCAGACC TTCCAAAACT CATCAACTCC 
ATGAATGTCC AGAAGCCAAA AGAAAATGAA ATTGTCCTCC TAAGTGGGCT AGCCTCTGGA 
AACCGCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGCGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT GGTGCAGGAG CGACAGCTCC ACCTGGAGAC CAGCGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCCCTG TCTTCGATTG AAGAAGATTT CCTTACAGCC 
TCAGAGCATT TGGAGGAAGA AATCGAGGTG GAAGACTGCA AAAGTGGTTT GGAAACTATA 
AATGTGTCAG CTAACGTTCT GGAAAGCAAA AGGCCAAAGG AAGCTACCCA GGAAGGATGG 
GATTACCACA AGGAAATATT GCATTGTGCT TTAGGTGAAA AACACATCAG AAAACACCGT 
ATGCCCTCAA TGAAAACAGA AAGGTCAAAG GAAAACACAG CAGAGAACAC AGCCCTGCAA 
AGTCTCAATC CTTCAGCCAG ACCATCACGC TTGAAAAGTG AAGTGGCGGG GAGCAAGCAG 
CCAGCCACGA ACTACTCTTA CCCCGAAAAT ATCAGAGGAG AAATAGAAAC ATCTCAGATG 
CTGTTCATCC CCAGGGATGC CTATCTCTCC ATGGTTTCTT CATGTGGTGT ACTCACTGAG 
CAGGGAAGCA ACCACAGAGA CCATGACGCC ACTCCAAACT CCCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA CCGCGGGCGA GTACGCTACC AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC GCAACCTACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
GTGGGAAGCG CTCTGCTCCC CAGTGGTTGT TGCACCAAAG ATATGGTGGT CCCTCGTTCA 
TGGAATGAGC TTCCTAAAAT TGTCATCGTG CAGAGCCCAG AGGGCAGCGA CACAGCCCCT 
GAGCCAAGTG TCTCCTCCTG GCCCGACCTG GAAGTGTCTG TCGAGACCCC GGGCATCTTT 
TCTGAAGAGA GCTCCAGCAG ACCCACTCAG AGTGCCCTAG AAGTTGCTTT GGCTTGTGCA 
GCCACTGTGA TTGGAACCAC TTCCAGTCCA CAGGCCACAG AAAGATTCAC CATGGAGCAA 
GAATCTCTGG TATCTACCTA CACCCAGAGA GGCAATGGGA TACAGCAAAC TCCAGTGCCC 
AGAGTATTCA TGGGACCTTC TACAACTGAA TACTCCTTCC CATCAGCTCT GTGTGGCATG 
ACCCAAGTGG CAAGCGCTGT TGCTGTCTGT GGCCTAGGGG AAAGAGAAGA GGTGACATAT 
TCTGTAGCTC CAAGTGACCT CCTGCCCACA CCTGGGGCTT CTGAAGAAAG ATCCTCCATT 
GGAAGTTTAG TGACAGAGGA AAGCACAGAA CTGGGGAAGG AAGCCATTGC AGAGGCTCTG 
CTCAGGGAGG CTACTCTGGT TTTAGCGAGG CCGAATGCAT ACAGCAGCGT CGGAGAGCTC 
TTGGAATCTG TGAACCGGAG GATCATAGAA ACCACTTCTA AAACCCAGAT GCTGTGCACA 
GAAAATGTTC GAAGGAATGA ACTGGCACAC ACCTTGTCCA ATGTCATCCT CAAGCATTCC 
ATTGATGAGC TGCACCAGAA AAATATCATG GCTCACCCCA CCGAGGAAAG GCATCCCGGT 
GGGACTCTGA ACACCTTAAT GGAAAGTGTG AACCAGTTGC TGCACAACGT GATGTGCTTC 
ACCTTCAGAA AGATGAATCA CATCGTAACA CTGGGTGAGC ATCCCGCTGG CCTTTCTAAG 
GAGACTGTCA CTTGGACAGA GTCAGGGCCC TTAGGTTGCC AGTCATTTGA TAAGGCTGCT 
GGTCATGCAT GGGTTAAAGC CTCGGCTTGC TCCAGCAGCC ATCCCTCTAG CAATGCACAC 
GGTACTGGCC TTGTCATCAG AGATCTTGTA GAAGATGCAT CTCCTAAGCC AGACAAGGGA 
GGAGCAAGGC CAGAACTGGT CAACAACCCC AGGCTGCAAT CTGAATTCTC ATGCAGTCAC 
AGAATGCTTG ATTCAACTGC TAGGACATTC CCCAAGGAAA TGTATCCAAA AGGGATTGTG 
GGAGAGGATA CAAGAAACCC TCTTCATACA CTAAGTTATG ACAGCAGTGA ACAAAGAACT 
TCTACAGATA TAGGAAAATT AACAACAGTA GGAGAGGTCC GTAGTACCTT CCAGGAATCT 
GAAGACAGCA TTGTTCCAAA AGCCCAAGAG AAACACACTT GTGCCACAAC TCTAAACAAC 
GAAGCTCAAA TTAACCTATC CTTGTTAGGG GATGACCTAG TGGTTCCTGA TCAATCTACA 
CTAGAGGCAA AGCAGTCCGA GGTCTATGGC ATCACAAACT TTGCGGAAGA ATTGGCAGAG 
ACTGTTGTCT CCATGGCAAC TGAAATCGCA GCAATCTGCC TTGACAACTC CCATGGAAAA 
CAGCCGTGGT TTTGTGCTTG GAAGAGAGGG AACGAATTTC TGATGACGCC TAACGCATCC 
TGCCGATCCT TGAAGAGGAA GAAGGAAAAC TCGGGCACGG GAAGCACTGT GAGGAAACAC 
AAGCCACCTC GCCTCAGTGA GATCAAGAGA AAAGCAGATG AGCACCCAGA GCTGAAAGAG 
AAGCTGATGA ACAGAGTCAT GGATGAGTCC ATGAACCTCG AAGACATTCC CGATTCCGTC 
AGTACTTTTG CAAATGAAGT GGCAGCCAAG ATCATGAACC TAACAGAATT CTCCACGGTG 
GATGGAGTGT GGCAAACCCA GAGTTGTTCC CGGAATCGGC TTCTGGGTGG TGATAGGTGG 
AACCGGCTGA AGGCCTCGAG CTGTGAAAGT ATTCCGGAGG AGGACTCTGA AGCCAGGGTC 
TTTGTAAACA GCCTGGGTTT AATGAGTACC TTAAGCCAAC CCGTTAGCAG GGCCAGCTCA 
GTCTCCAAAC AGTCCAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAA 
CAGATGGAAA ACGAAGGGAG GGGGTTCGAG TTGCTGCTGG ATTACTACGC TGGCAAAAAT 
GCCAGCAGTA TCCTGAACTC AGCAATCCAG CAGGCATGCC AGAAAAACGA CCACCTCAAC 
GTAAGACCAA GCTGCCCCTC TAAGCAGTCC AGCACAGAAA GCATAACAGA GGAGTTCTAT 
AAGTACATGC TGAGAGACAT TGCCAAAGAA AACAAAGATG GCGCCCTGTC CAGACGAAGC 
AGCCATGATT GGAGCACGGG CTTGCTGTCT CCTTCCGCGC GATCCCCACT GTGTTACAGG 
CAGTCATCTA TGCCTGACAG CAGGTCCCCG TGCTCCAGGT TAACAGTAAA TGCACCTATC 
AAAGCCAACT CCTTAGATGG TTTTGCCCAA AACTGCCCTC AAGATTCTAT AAATGTACAG 
CCAGTCAGTA GGGCTTCCTC GTCTGGCCTC TGCAAATCAG ACTCCTGTTT ATATCGCCGA 
AGTGGGACTG ACCAGATCAC AAACATGCTA ATTCATGAAA CATGGGCCAG CTCCATTGAG 
GCTCTCATGA GAAAGAACAA AATCATTGCT GATGACGGCG AGGCAGCTAA CGCTAGCCCT 
GGTCCTGTTT CTGGCGGCTC GCCTTCGCAA GTAGAGAAGT GTGCCAACAG ACTAGTCACC 
GGCACAGGAC ACAAGGGACC AGCTCTACTT GTACAGGAGT CTGTTGATTA CCAGAGAAAG 
GATGCTGTCA CTGAAGGCAA TTGTTCCCCA GTGTCATCTC CAAGCAAAAT GGCTCCTGTT 
AAAAAGCCCA GTGGTTTTGA TCCTACAAGA GAAACCTCTG CATGCCACAA TGCTGTTGCT 
CTCAAAAGCC CCAGGCGGTC TCTTTGCTCA AGGGAGGTGC CTTTGATTCA GATAGAGACC 
GATCAGAAAG AAGAATGCGT TGGAGAATCT GAAACCTTAC TTCCCCAAAG TGGCTCCCTA 
GAGGAAGCAG AAGGGCCCCA GCCTGAGGAA ACCATCCCAG ATGTGGCCAG AAGCGAAGAC 
ACAGCCCTGA GCGCCTGTCA AAGCTCTCAT GACAGCCTTG AGACCAGAGA AGAATTAGAA 
GTGGATGTCT TGAAAGAAGA CATAACCCTA GATGAGTCCC GGAACCCTCC TAGCAGCAGC 
GAAGAGAGTA CAGGCAGCTG GTCCCAGCTG GCCAATGAGG AGGACAACCC AGATGACACA 
AGTAGCTTTC TGCAGCTCAG TGAGCGGTCC ATGAGTGAAT TAGTAGAAGA AAAGGAGATT 
CTTAAAGAAC AATCAGAAAG CGTAAAGGAA CGTGCCTCTG GACTGCCAGG GAGAGCAGCC 
AGCCCCCAGA GGAGTCTACT GGTGATCAAC TTTGACCTGG AGCCAGAGTG TCCTGATGCT 
GAGCTGCGAG CCACTCTGCA GTGGATAGCT GCCTCCGAGC TGGGCATCCC AACAATCTAC 
TTTAAGAAAT CTCAGGAAAG CAGAATTGAA AAGTTTCTAG ATGTTGTGAA GCTTGTTCAT 
CAGAAGTCCT GGAAGGTGGG AGATATATTT CATGCCGTTG TCCAGTACTG CAAAATGCAT 
GCAGAGCAGA AAGAGGGAAC TCCGAGTCTC TTTGACTGGC TCCTGGAACT AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245296.1
CDS	313..5409
Translation	MSTPGFLCKAMWVVEPNSATCLSAAPPVKRHCQESSNVESPLMPEDSEPQQITSSAAGSLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDGCSTENLQQKLVNVSPDLPKLINSMNVQKPKENEIVLLSGLASGNRQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETSVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVEDCKSGLETINVSANVLESKRPKEATQEGWDYHKEILHCALGEKHIRKHRMPSMKTERSKENTAENTALQSLNPSARPSRLKSEVAGSKQPATNYSYPENIRGEIETSQMLFIPRDAYLSMVSSCGVLTEQGSNHRDHDATPNSLPPVQNGEATAGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSVGSALLPSGCCTKDMVVPRSWNELPKIVIVQSPEGSDTAPEPSVSSWPDLEVSVETPGIFSEESSSRPTQSALEVALACAATVIGTTSSPQATERFTMEQESLVSTYTQRGNGIQQTPVPRVFMGPSTTEYSFPSALCGMTQVASAVAVCGLGEREEVTYSVAPSDLLPTPGASEERSSIGSLVTEESTELGKEAIAEALLREATLVLARPNAYSSVGELLESVNRRIIETTSKTQMLCTENVRRNELAHTLSNVILKHSIDELHQKNIMAHPTEERHPGGTLNTLMESVNQLLHNVMCFTFRKMNHIVTLGEHPAGLSKETVTWTESGPLGCQSFDKAAGHAWVKASACSSSHPSSNAHGTGLVIRDLVEDASPKPDKGGARPELVNNPRLQSEFSCSHRMLDSTARTFPKEMYPKGIVGEDTRNPLHTLSYDSSEQRTSTDIGKLTTVGEVRSTFQESEDSIVPKAQEKHTCATTLNNEAQINLSLLGDDLVVPDQSTLEAKQSEVYGITNFAEELAETVVSMATEIAAICLDNSHGKQPWFCAWKRGNEFLMTPNASCRSLKRKKENSGTGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSTVDGVWQTQSCSRNRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSILNSAIQQACQKNDHLNVRPSCPSKQSSTESITEEFYKYMLRDIAKENKDGALSRRSSHDWSTGLLSPSARSPLCYRQSSMPDSRSPCSRLTVNAPIKANSLDGFAQNCPQDSINVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDGEAANASPGPVSGGSPSQVEKCANRLVTGTGHKGPALLVQESVDYQRKDAVTEGNCSPVSSPSKMAPVKKPSGFDPTRETSACHNAVALKSPRRSLCSREVPLIQIETDQKEECVGESETLLPQSGSLEEAEGPQPEETIPDVARSEDTALSACQSSHDSLETREELEVDVLKEDITLDESRNPPSSSEESTGSWSQLANEEDNPDDTSSFLQLSERSMSELVEEKEILKEQSESVKERASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVHQKSWKVGDIFHAVVQYCKMHAEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006245234.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245234.3

ORF Insert Sequence:

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCCGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC CCGAGGACTC GGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAGCCTG 
GCTGGCTCCA TCACGGCCTG TAAGAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGT TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGACGAGTCT 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAGGATGG CTGCAGCACA 
GAGAACCTGC AGCAGAAACT GGTCAACGTT TCACCAGACC TTCCAAAACT CATCAACTCC 
ATGAATGTCC AGAAGCCAAA AGAAAATGAA ATTGTCCTCC TAAGTGGGCT AGCCTCTGGA 
AACCGCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGCGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT GGTGCAGGAG CGACAGCTCC ACCTGGAGAC CAGCGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCCCTG TCTTCGATTG AAGAAGATTT CCTTACAGCC 
TCAGAGCATT TGGAGGAAGA AATCGAGGTG GAAGACTGCA AAAGTGGTTT GGAAACTATA 
AATGTGTCAG CTAACGTTCT GGAAAGCAAA AGGCCAAAGG AAGCTACCCA GGAAGGATGG 
GATTACCACA AGGAAATATT GCATTGTGCT TTAGGTGAAA AACACATCAG AAAACACCGT 
ATGCCCTCAA TGAAAACAGA AAGGTCAAAG GAAAACACAG CAGAGAACAC AGCCCTGCAA 
AGTCTCAATC CTTCAGCCAG ACCATCACGC TTGAAAAGTG AAGTGGCGGG GAGCAAGCAG 
CCAGCCACGA ACTACTCTTA CCCCGAAAAT ATCAGAGGAG AAATAGAAAC ATCTCAGATG 
CTGTTCATCC CCAGGGATGC CTATCTCTCC ATGGTTTCTT CATGTGGTGT ACTCACTGAG 
CAGGGAAGCA ACCACAGAGA CCATGACGCC ACTCCAAACT CCCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA CCGCGGGCGA GTACGCTACC AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC GCAACCTACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
GTGGGAAGCG CTCTGCTCCC CAGTGGTTGT TGCACCAAAG ATATGGTGGT CCCTCGTTCA 
TGGAATGAGC TTCCTAAAAT TGTCATCGTG CAGAGCCCAG AGGGCAGCGA CACAGCCCCT 
GAGCCAAGTG TCTCCTCCTG GCCCGACCTG GAAGTGTCTG TCGAGACCCC GGGCATCTTT 
TCTGAAGAGA GCTCCAGCAG ACCCACTCAG AGTGCCCTAG AAGTTGCTTT GGCTTGTGCA 
GCCACTGTGA TTGGAACCAC TTCCAGTCCA CAGGCCACAG AAAGATTCAC CATGGAGCAA 
GAATCTCTGG TATCTACCTA CACCCAGAGA GGCAATGGGA TACAGCAAAC TCCAGTGCCC 
AGAGTATTCA TGGGACCTTC TACAACTGAA TACTCCTTCC CATCAGCTCT GTGTGGCATG 
ACCCAAGTGG CAAGCGCTGT TGCTGTCTGT GGCCTAGGGG AAAGAGAAGA GGTGACATAT 
TCTGTAGCTC CAAGTGACCT CCTGCCCACA CCTGGGGCTT CTGAAGAAAG ATCCTCCATT 
GGAAGTTTAG TGACAGAGGA AAGCACAGAA CTGGGGAAGG AAGCCATTGC AGAGGCTCTG 
CTCAGGGAGG CTACTCTGGT TTTAGCGAGG CCGAATGCAT ACAGCAGCGT CGGAGAGCTC 
TTGGAATCTG TGAACCGGAG GATCATAGAA ACCACTTCTA AAACCCAGAT GCTGTGCACA 
GAAAATGTTC GAAGGAATGA ACTGGCACAC ACCTTGTCCA ATGTCATCCT CAAGCATTCC 
ATTGATGAGC TGCACCAGAA AAATATCATG GCTCACCCCA CCGAGGAAAG GCATCCCGGT 
GGGACTCTGA ACACCTTAAT GGAAAGTGTG AACCAGTTGC TGCACAACGT GATGTGCTTC 
ACCTTCAGAA AGATGAATCA CATCGTAACA CTGGGTGAGC ATCCCGCTGG CCTTTCTAAG 
GAGACTGTCA CTTGGACAGA GTCAGGGCCC TTAGGTTGCC AGTCATTTGA TAAGGCTGCT 
GGTCATGCAT GGGTTAAAGC CTCGGCTTGC TCCAGCAGCC ATCCCTCTAG CAATGCACAC 
GGTACTGGCC TTGTCATCAG AGATCTTGTA GAAGATGCAT CTCCTAAGCC AGACAAGGGA 
GGAGCAAGGC CAGAACTGGT CAACAACCCC AGGCTGCAAT CTGAATTCTC ATGCAGTCAC 
AGAATGCTTG ATTCAACTGC TAGGACATTC CCCAAGGAAA TGTATCCAAA AGGGATTGTG 
GGAGAGGATA CAAGAAACCC TCTTCATACA CTAAGTTATG ACAGCAGTGA ACAAAGAACT 
TCTACAGATA TAGGAAAATT AACAACAGTA GGAGAGGTCC GTAGTACCTT CCAGGAATCT 
GAAGACAGCA TTGTTCCAAA AGCCCAAGAG AAACACACTT GTGCCACAAC TCTAAACAAC 
GAAGCTCAAA TTAACCTATC CTTGTTAGGG GATGACCTAG TGGTTCCTGA TCAATCTACA 
CTAGAGGCAA AGCAGTCCGA GGTCTATGGC ATCACAAACT TTGCGGAAGA ATTGGCAGAG 
ACTGTTGTCT CCATGGCAAC TGAAATCGCA GCAATCTGCC TTGACAACTC CCATGGAAAA 
CAGCCGTGGT TTTGTGCTTG GAAGAGAGGG AACGAATTTC TGATGACGCC TAACGCATCC 
TGCCGATCCT TGAAGAGGAA GAAGGAAAAC TCGGGCACGG GAAGCACTGT GAGGAAACAC 
AAGCCACCTC GCCTCAGTGA GATCAAGAGA AAAGCAGATG AGCACCCAGA GCTGAAAGAG 
AAGCTGATGA ACAGAGTCAT GGATGAGTCC ATGAACCTCG AAGACATTCC CGATTCCGTC 
AGTACTTTTG CAAATGAAGT GGCAGCCAAG ATCATGAACC TAACAGAATT CTCCACGGTG 
GATGGAGTGT GGCAAACCCA GAGTTGTTCC CGGAATCGGC TTCTGGGTGG TGATAGGTGG 
AACCGGCTGA AGGCCTCGAG CTGTGAAAGT ATTCCGGAGG AGGACTCTGA AGCCAGGGTC 
TTTGTAAACA GCCTGGGTTT AATGAGTACC TTAAGCCAAC CCGTTAGCAG GGCCAGCTCA 
GTCTCCAAAC AGTCCAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAA 
CAGATGGAAA ACGAAGGGAG GGGGTTCGAG TTGCTGCTGG ATTACTACGC TGGCAAAAAT 
GCCAGCAGTA TCCTGAACTC AGCAATCCAG CAGGCATGCC AGAAAAACGA CCACCTCAAC 
GTAAGACCAA GCTGCCCCTC TAAGCAGTCC AGCACAGAAA GCATAACAGA GGAGTTCTAT 
AAGTACATGC TGAGAGACAT TGCCAAAGAA AACAAAGATG GCGCCCTGTC CAGACGAAGC 
AGCCATGATT GGAGCACGGG CTTGCTGTCT CCTTCCGCGC GATCCCCACT GTGTTACAGG 
CAGTCATCTA TGCCTGACAG CAGGTCCCCG TGCTCCAGGT TAACAGTAAA TGCACCTATC 
AAAGCCAACT CCTTAGATGG TTTTGCCCAA AACTGCCCTC AAGATTCTAT AAATGTACAG 
CCAGTCAGTA GGGCTTCCTC GTCTGGCCTC TGCAAATCAG ACTCCTGTTT ATATCGCCGA 
AGTGGGACTG ACCAGATCAC AAACATGCTA ATTCATGAAA CATGGGCCAG CTCCATTGAG 
GCTCTCATGA GAAAGAACAA AATCATTGCT GATGACGGCG AGGCAGCTAA CGCTAGCCCT 
GGTCCTGTTT CTGGCGGCTC GCCTTCGCAA GTAGAGAAGT GTGCCAACAG ACTAGTCACC 
GGCACAGGAC ACAAGGGACC AGCTCTACTT GTACAGGAGT CTGTTGATTA CCAGAGAAAG 
GATGCTGTCA CTGAAGGCAA TTGTTCCCCA GTGTCATCTC CAAGCAAAAT GGCTCCTGTT 
AAAAAGCCCA GTGGTTTTGA TCCTACAAGA GAAACCTCTG CATGCCACAA TGCTGTTGCT 
CTCAAAAGCC CCAGGCGGTC TCTTTGCTCA AGGGAGGTGC CTTTGATTCA GATAGAGACC 
GATCAGAAAG AAGAATGCGT TGGAGAATCT GAAACCTTAC TTCCCCAAAG TGGCTCCCTA 
GAGGAAGCAG AAGGGCCCCA GCCTGAGGAA ACCATCCCAG ATGTGGCCAG AAGCGAAGAC 
ACAGCCCTGA GCGCCTGTCA AAGCTCTCAT GACAGCCTTG AGACCAGAGA AGAATTAGAA 
GTGGATGTCT TGAAAGAAGA CATAACCCTA GATGAGTCCC GGAACCCTCC TAGCAGCAGC 
GAAGAGAGTA CAGGCAGCTG GTCCCAGCTG GCCAATGAGG AGGACAACCC AGATGACACA 
AGTAGCTTTC TGCAGCTCAG TGAGCGGTCC ATGAGTGAAT TAGTAGAAGA AAAGGAGATT 
CTTAAAGAAC AATCAGAAAG CGTAAAGGAA CGTGCCTCTG GACTGCCAGG GAGAGCAGCC 
AGCCCCCAGA GGAGTCTACT GGTGATCAAC TTTGACCTGG AGCCAGAGTG TCCTGATGCT 
GAGCTGCGAG CCACTCTGCA GTGGATAGCT GCCTCCGAGC TGGGCATCCC AACAATCTAC 
TTTAAGAAAT CTCAGGAAAG CAGAATTGAA AAGTTTCTAG ATGTTGTGAA GCTTGTTCAT 
CAGAAGTCCT GGAAGGTGGG AGATATATTT CATGCCGTTG TCCAGTACTG CAAAATGCAT 
GCAGAGCAGA AAGAGGGAAC TCCGAGTCTC TTTGACTGGC TCCTGGAACT AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( XM_008767251.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765473.1 Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X4, mRNA.

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CloneID	ORa47550
Clone ID Related Accession (Same CDS sequence)	XM_008767251.2
Accession Version	XM_008767251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein SPHKAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACTG AGTCTCTACT TCAGTCTCAT GTCTGGAAAA GCAACAACGT GGAATCACCA 
CTGATGCCCG AGGACTCGGA GCCACAGCAG ATCACCAGCA GTGCAGCAGG CAGCCTGGCT 
GGCTCCATCA CGGCCTGTAA GAAGGTTCTT CGAAGCAATA GCTTGCTGGA GTCCACAGAC 
TACTGGTTGC AGAACCAGAG GACGCCTTGC CAGATTGGGT TTGTGGAAGA CGAGTCTGAA 
AACTGTGCTT CTGTCTGCTT TGTGAACCTG GATGTCAACA AGGATGGCTG CAGCACAGAG 
AACCTGCAGC AGAAACTGGT CAACGTTTCA CCAGACCTTC CAAAACTCAT CAACTCCATG 
AATGTCCAGA AGCCAAAAGA AAATGAAATT GTCCTCCTAA GTGGGCTAGC CTCTGGAAAC 
CGCCAGGCAG ATTTTGATGT TTCACAGTGC CCTTGGCTAC CAGATATCTG CTTGGTCCAG 
TGCGCAAGAG GGAACAGACC AAACAGTACC AACTGCATCA TCTTTGAGAT CAACAAGTTC 
TTAATTGGTC TGGAAGTGGT GCAGGAGCGA CAGCTCCACC TGGAGACCAG CGTGTTGAAG 
CTGGAGGATG ATACAAACTG TTCCCTGTCT TCGATTGAAG AAGATTTCCT TACAGCCTCA 
GAGCATTTGG AGGAAGAAAT CGAGGTGGAA GACTGCAAAA GTGGTTTGGA AACTATAAAT 
GTGTCAGCTA ACGTTCTGGA AAGCAAAAGG CCAAAGGAAG CTACCCAGGA AGGATGGGAT 
TACCACAAGG AAATATTGCA TTGTGCTTTA GGTGAAAAAC ACATCAGAAA ACACCGTATG 
CCCTCAATGA AAACAGAAAG GTCAAAGGAA AACACAGCAG AGAACACAGC CCTGCAAAGT 
CTCAATCCTT CAGCCAGACC ATCACGCTTG AAAAGTGAAG TGGCGGGGAG CAAGCAGCCA 
GCCACGAACT ACTCTTACCC CGAAAATATC AGAGGAGAAA TAGAAACATC TCAGATGCTG 
TTCATCCCCA GGGATGCCTA TCTCTCCATG GTTTCTTCAT GTGGTGTACT CACTGAGCAG 
GGAAGCAACC ACAGAGACCA TGACGCCACT CCAAACTCCC TTCCTCCCGT TCAAAATGGA 
GAAGCCACCG CGGGCGAGTA CGCTACCAAC TTAGCAGAAT CTGTAATGCA AGATGCCTTC 
ATCCGATTGT CTCAGTCGCA ACCTACACTC CCCCAGGAAT CTGCAGTCAG TTTCTCTGTG 
GGAAGCGCTC TGCTCCCCAG TGGTTGTTGC ACCAAAGATA TGGTGGTCCC TCGTTCATGG 
AATGAGCTTC CTAAAATTGT CATCGTGCAG AGCCCAGAGG GCAGCGACAC AGCCCCTGAG 
CCAAGTGTCT CCTCCTGGCC CGACCTGGAA GTGTCTGTCG AGACCCCGGG CATCTTTTCT 
GAAGAGAGCT CCAGCAGACC CACTCAGAGT GCCCTAGAAG TTGCTTTGGC TTGTGCAGCC 
ACTGTGATTG GAACCACTTC CAGTCCACAG GCCACAGAAA GATTCACCAT GGAGCAAGAA 
TCTCTGGTAT CTACCTACAC CCAGAGAGGC AATGGGATAC AGCAAACTCC AGTGCCCAGA 
GTATTCATGG GACCTTCTAC AACTGAATAC TCCTTCCCAT CAGCTCTGTG TGGCATGACC 
CAAGTGGCAA GCGCTGTTGC TGTCTGTGGC CTAGGGGAAA GAGAAGAGGT GACATATTCT 
GTAGCTCCAA GTGACCTCCT GCCCACACCT GGGGCTTCTG AAGAAAGATC CTCCATTGGA 
AGTTTAGTGA CAGAGGAAAG CACAGAACTG GGGAAGGAAG CCATTGCAGA GGCTCTGCTC 
AGGGAGGCTA CTCTGGTTTT AGCGAGGCCG AATGCATACA GCAGCGTCGG AGAGCTCTTG 
GAATCTGTGA ACCGGAGGAT CATAGAAACC ACTTCTAAAA CCCAGATGCT GTGCACAGAA 
AATGTTCGAA GGAATGAACT GGCACACACC TTGTCCAATG TCATCCTCAA GCATTCCATT 
GATGAGCTGC ACCAGAAAAA TATCATGGCT CACCCCACCG AGGAAAGGCA TCCCGGTGGG 
ACTCTGAACA CCTTAATGGA AAGTGTGAAC CAGTTGCTGC ACAACGTGAT GTGCTTCACC 
TTCAGAAAGA TGAATCACAT CGTAACACTG GGTGAGCATC CCGCTGGCCT TTCTAAGGAG 
ACTGTCACTT GGACAGAGTC AGGGCCCTTA GGTTGCCAGT CATTTGATAA GGCTGCTGGT 
CATGCATGGG TTAAAGCCTC GGCTTGCTCC AGCAGCCATC CCTCTAGCAA TGCACACGGT 
ACTGGCCTTG TCATCAGAGA TCTTGTAGAA GATGCATCTC CTAAGCCAGA CAAGGGAGGA 
GCAAGGCCAG AACTGGTCAA CAACCCCAGG CTGCAATCTG AATTCTCATG CAGTCACAGA 
ATGCTTGATT CAACTGCTAG GACATTCCCC AAGGAAATGT ATCCAAAAGG GATTGTGGGA 
GAGGATACAA GAAACCCTCT TCATACACTA AGTTATGACA GCAGTGAACA AAGAACTTCT 
ACAGATATAG GAAAATTAAC AACAGTAGGA GAGGTCCGTA GTACCTTCCA GGAATCTGAA 
GACAGCATTG TTCCAAAAGC CCAAGAGAAA CACACTTGTG CCACAACTCT AAACAACGAA 
GCTCAAATTA ACCTATCCTT GTTAGGGGAT GACCTAGTGG TTCCTGATCA ATCTACACTA 
GAGGCAAAGC AGTCCGAGGT CTATGGCATC ACAAACTTTG CGGAAGAATT GGCAGAGACT 
GTTGTCTCCA TGGCAACTGA AATCGCAGCA ATCTGCCTTG ACAACTCCCA TGGAAAACAG 
CCGTGGTTTT GTGCTTGGAA GAGAGGGAAC GAATTTCTGA TGACGCCTAA CGCATCCTGC 
CGATCCTTGA AGAGGAAGAA GGAAAACTCG GGCACGGGAA GCACTGTGAG GAAACACAAG 
CCACCTCGCC TCAGTGAGAT CAAGAGAAAA GCAGATGAGC ACCCAGAGCT GAAAGAGAAG 
CTGATGAACA GAGTCATGGA TGAGTCCATG AACCTCGAAG ACATTCCCGA TTCCGTCAGT 
ACTTTTGCAA ATGAAGTGGC AGCCAAGATC ATGAACCTAA CAGAATTCTC CACGGTGGAT 
GGAGTGTGGC AAACCCAGAG TTGTTCCCGG AATCGGCTTC TGGGTGGTGA TAGGTGGAAC 
CGGCTGAAGG CCTCGAGCTG TGAAAGTATT CCGGAGGAGG ACTCTGAAGC CAGGGTCTTT 
GTAAACAGCC TGGGTTTAAT GAGTACCTTA AGCCAACCCG TTAGCAGGGC CAGCTCAGTC 
TCCAAACAGT CCAGCTGTGA GAGCATCACC GATGAGTTTT CCAGGTTCAT GGTGAAACAG 
ATGGAAAACG AAGGGAGGGG GTTCGAGTTG CTGCTGGATT ACTACGCTGG CAAAAATGCC 
AGCAGTATCC TGAACTCAGC AATCCAGCAG GCATGCCAGA AAAACGACCA CCTCAACGTA 
AGACCAAGCT GCCCCTCTAA GCAGTCCAGC ACAGAAAGCA TAACAGAGGA GTTCTATAAG 
TACATGCTGA GAGACATTGC CAAAGAAAAC AAAGATGGCG CCCTGTCCAG ACGAAGCAGC 
CATGATTGGA GCACGGGCTT GCTGTCTCCT TCCGCGCGAT CCCCACTGTG TTACAGGCAG 
TCATCTATGC CTGACAGCAG GTCCCCGTGC TCCAGGTTAA CAGTAAATGC ACCTATCAAA 
GCCAACTCCT TAGATGGTTT TGCCCAAAAC TGCCCTCAAG ATTCTATAAA TGTACAGCCA 
GTCAGTAGGG CTTCCTCGTC TGGCCTCTGC AAATCAGACT CCTGTTTATA TCGCCGAAGT 
GGGACTGACC AGATCACAAA CATGCTAATT CATGAAACAT GGGCCAGCTC CATTGAGGCT 
CTCATGAGAA AGAACAAAAT CATTGCTGAT GACGGCGAGG CAGCTAACGC TAGCCCTGGT 
CCTGTTTCTG GCGGCTCGCC TTCGCAAGTA GAGAAGTGTG CCAACAGACT AGTCACCGGC 
ACAGGACACA AGGGACCAGC TCTACTTGTA CAGGAGTCTG TTGATTACCA GAGAAAGGAT 
GCTGTCACTG AAGGCAATTG TTCCCCAGTG TCATCTCCAA GCAAAATGGC TCCTGTTAAA 
AAGCCCAGTG GTTTTGATCC TACAAGAGAA ACCTCTGCAT GCCACAATGC TGTTGCTCTC 
AAAAGCCCCA GGCGGTCTCT TTGCTCAAGG GAGGTGCCTT TGATTCAGAT AGAGACCGAT 
CAGAAAGAAG AATGCGTTGG AGAATCTGAA ACCTTACTTC CCCAAAGTGG CTCCCTAGAG 
GAAGCAGAAG GGCCCCAGCC TGAGGAAACC ATCCCAGATG TGGCCAGAAG CGAAGACACA 
GCCCTGAGCG CCTGTCAAAG CTCTCATGAC AGCCTTGAGA CCAGAGAAGA ATTAGAAGTG 
GATGTCTTGA AAGAAGACAT AACCCTAGAT GAGTCCCGGA ACCCTCCTAG CAGCAGCGAA 
GAGAGTACAG GCAGCTGGTC CCAGCTGGCC AATGAGGAGG ACAACCCAGA TGACACAAGT 
AGCTTTCTGC AGCTCAGTGA GCGGTCCATG AGCAATGGCA ACACTAGTGG CACTAGCAGT 
CTTGGCATTA TGGACCTGGA CATTTATCAG GAAAGCATAC CGTCTTCTCC CATGATTAAT 
GAATTAGTAG AAGAAAAGGA GATTCTTAAA GAACAATCAG AAAGCGTAAA GGAACGTGCC 
TCTGGACTGC CAGGGAGAGC AGCCAGCCCC CAGAGGAGTC TACTGGTGAT CAACTTTGAC 
CTGGAGCCAG AGTGTCCTGA TGCTGAGCTG CGAGCCACTC TGCAGTGGAT AGCTGCCTCC 
GAGCTGGGCA TCCCAACAAT CTACTTTAAG AAATCTCAGG AAAGCAGAAT TGAAAAGTTT 
CTAGATGTTG TGAAGCTTGT TCATCAGAAG TCCTGGAAGG TGGGAGATAT ATTTCATGCC 
GTTGTCCAGT ACTGCAAAAT GCATGCAGAG CAGAAAGAGG GAACTCCGAG TCTCTTTGAC 
TGGCTCCTGG AACTAGGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765473.1
CDS	699..5819
Translation	MITESLLQSHVWKSNNVESPLMPEDSEPQQITSSAAGSLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDGCSTENLQQKLVNVSPDLPKLINSMNVQKPKENEIVLLSGLASGNRQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETSVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVEDCKSGLETINVSANVLESKRPKEATQEGWDYHKEILHCALGEKHIRKHRMPSMKTERSKENTAENTALQSLNPSARPSRLKSEVAGSKQPATNYSYPENIRGEIETSQMLFIPRDAYLSMVSSCGVLTEQGSNHRDHDATPNSLPPVQNGEATAGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSVGSALLPSGCCTKDMVVPRSWNELPKIVIVQSPEGSDTAPEPSVSSWPDLEVSVETPGIFSEESSSRPTQSALEVALACAATVIGTTSSPQATERFTMEQESLVSTYTQRGNGIQQTPVPRVFMGPSTTEYSFPSALCGMTQVASAVAVCGLGEREEVTYSVAPSDLLPTPGASEERSSIGSLVTEESTELGKEAIAEALLREATLVLARPNAYSSVGELLESVNRRIIETTSKTQMLCTENVRRNELAHTLSNVILKHSIDELHQKNIMAHPTEERHPGGTLNTLMESVNQLLHNVMCFTFRKMNHIVTLGEHPAGLSKETVTWTESGPLGCQSFDKAAGHAWVKASACSSSHPSSNAHGTGLVIRDLVEDASPKPDKGGARPELVNNPRLQSEFSCSHRMLDSTARTFPKEMYPKGIVGEDTRNPLHTLSYDSSEQRTSTDIGKLTTVGEVRSTFQESEDSIVPKAQEKHTCATTLNNEAQINLSLLGDDLVVPDQSTLEAKQSEVYGITNFAEELAETVVSMATEIAAICLDNSHGKQPWFCAWKRGNEFLMTPNASCRSLKRKKENSGTGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSTVDGVWQTQSCSRNRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSILNSAIQQACQKNDHLNVRPSCPSKQSSTESITEEFYKYMLRDIAKENKDGALSRRSSHDWSTGLLSPSARSPLCYRQSSMPDSRSPCSRLTVNAPIKANSLDGFAQNCPQDSINVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDGEAANASPGPVSGGSPSQVEKCANRLVTGTGHKGPALLVQESVDYQRKDAVTEGNCSPVSSPSKMAPVKKPSGFDPTRETSACHNAVALKSPRRSLCSREVPLIQIETDQKEECVGESETLLPQSGSLEEAEGPQPEETIPDVARSEDTALSACQSSHDSLETREELEVDVLKEDITLDESRNPPSSSEESTGSWSQLANEEDNPDDTSSFLQLSERSMSNGNTSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESVKERASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVHQKSWKVGDIFHAVVQYCKMHAEQKEGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_008767251.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767251.2

ORF Insert Sequence:

ATGATCACTG AGTCTCTACT TCAGTCTCAT GTCTGGAAAA GCAACAACGT GGAATCACCA 
CTGATGCCCG AGGACTCGGA GCCACAGCAG ATCACCAGCA GTGCAGCAGG CAGCCTGGCT 
GGCTCCATCA CGGCCTGTAA GAAGGTTCTT CGAAGCAATA GCTTGCTGGA GTCCACAGAC 
TACTGGTTGC AGAACCAGAG GACGCCTTGC CAGATTGGGT TTGTGGAAGA CGAGTCTGAA 
AACTGTGCTT CTGTCTGCTT TGTGAACCTG GATGTCAACA AGGATGGCTG CAGCACAGAG 
AACCTGCAGC AGAAACTGGT CAACGTTTCA CCAGACCTTC CAAAACTCAT CAACTCCATG 
AATGTCCAGA AGCCAAAAGA AAATGAAATT GTCCTCCTAA GTGGGCTAGC CTCTGGAAAC 
CGCCAGGCAG ATTTTGATGT TTCACAGTGC CCTTGGCTAC CAGATATCTG CTTGGTCCAG 
TGCGCAAGAG GGAACAGACC AAACAGTACC AACTGCATCA TCTTTGAGAT CAACAAGTTC 
TTAATTGGTC TGGAAGTGGT GCAGGAGCGA CAGCTCCACC TGGAGACCAG CGTGTTGAAG 
CTGGAGGATG ATACAAACTG TTCCCTGTCT TCGATTGAAG AAGATTTCCT TACAGCCTCA 
GAGCATTTGG AGGAAGAAAT CGAGGTGGAA GACTGCAAAA GTGGTTTGGA AACTATAAAT 
GTGTCAGCTA ACGTTCTGGA AAGCAAAAGG CCAAAGGAAG CTACCCAGGA AGGATGGGAT 
TACCACAAGG AAATATTGCA TTGTGCTTTA GGTGAAAAAC ACATCAGAAA ACACCGTATG 
CCCTCAATGA AAACAGAAAG GTCAAAGGAA AACACAGCAG AGAACACAGC CCTGCAAAGT 
CTCAATCCTT CAGCCAGACC ATCACGCTTG AAAAGTGAAG TGGCGGGGAG CAAGCAGCCA 
GCCACGAACT ACTCTTACCC CGAAAATATC AGAGGAGAAA TAGAAACATC TCAGATGCTG 
TTCATCCCCA GGGATGCCTA TCTCTCCATG GTTTCTTCAT GTGGTGTACT CACTGAGCAG 
GGAAGCAACC ACAGAGACCA TGACGCCACT CCAAACTCCC TTCCTCCCGT TCAAAATGGA 
GAAGCCACCG CGGGCGAGTA CGCTACCAAC TTAGCAGAAT CTGTAATGCA AGATGCCTTC 
ATCCGATTGT CTCAGTCGCA ACCTACACTC CCCCAGGAAT CTGCAGTCAG TTTCTCTGTG 
GGAAGCGCTC TGCTCCCCAG TGGTTGTTGC ACCAAAGATA TGGTGGTCCC TCGTTCATGG 
AATGAGCTTC CTAAAATTGT CATCGTGCAG AGCCCAGAGG GCAGCGACAC AGCCCCTGAG 
CCAAGTGTCT CCTCCTGGCC CGACCTGGAA GTGTCTGTCG AGACCCCGGG CATCTTTTCT 
GAAGAGAGCT CCAGCAGACC CACTCAGAGT GCCCTAGAAG TTGCTTTGGC TTGTGCAGCC 
ACTGTGATTG GAACCACTTC CAGTCCACAG GCCACAGAAA GATTCACCAT GGAGCAAGAA 
TCTCTGGTAT CTACCTACAC CCAGAGAGGC AATGGGATAC AGCAAACTCC AGTGCCCAGA 
GTATTCATGG GACCTTCTAC AACTGAATAC TCCTTCCCAT CAGCTCTGTG TGGCATGACC 
CAAGTGGCAA GCGCTGTTGC TGTCTGTGGC CTAGGGGAAA GAGAAGAGGT GACATATTCT 
GTAGCTCCAA GTGACCTCCT GCCCACACCT GGGGCTTCTG AAGAAAGATC CTCCATTGGA 
AGTTTAGTGA CAGAGGAAAG CACAGAACTG GGGAAGGAAG CCATTGCAGA GGCTCTGCTC 
AGGGAGGCTA CTCTGGTTTT AGCGAGGCCG AATGCATACA GCAGCGTCGG AGAGCTCTTG 
GAATCTGTGA ACCGGAGGAT CATAGAAACC ACTTCTAAAA CCCAGATGCT GTGCACAGAA 
AATGTTCGAA GGAATGAACT GGCACACACC TTGTCCAATG TCATCCTCAA GCATTCCATT 
GATGAGCTGC ACCAGAAAAA TATCATGGCT CACCCCACCG AGGAAAGGCA TCCCGGTGGG 
ACTCTGAACA CCTTAATGGA AAGTGTGAAC CAGTTGCTGC ACAACGTGAT GTGCTTCACC 
TTCAGAAAGA TGAATCACAT CGTAACACTG GGTGAGCATC CCGCTGGCCT TTCTAAGGAG 
ACTGTCACTT GGACAGAGTC AGGGCCCTTA GGTTGCCAGT CATTTGATAA GGCTGCTGGT 
CATGCATGGG TTAAAGCCTC GGCTTGCTCC AGCAGCCATC CCTCTAGCAA TGCACACGGT 
ACTGGCCTTG TCATCAGAGA TCTTGTAGAA GATGCATCTC CTAAGCCAGA CAAGGGAGGA 
GCAAGGCCAG AACTGGTCAA CAACCCCAGG CTGCAATCTG AATTCTCATG CAGTCACAGA 
ATGCTTGATT CAACTGCTAG GACATTCCCC AAGGAAATGT ATCCAAAAGG GATTGTGGGA 
GAGGATACAA GAAACCCTCT TCATACACTA AGTTATGACA GCAGTGAACA AAGAACTTCT 
ACAGATATAG GAAAATTAAC AACAGTAGGA GAGGTCCGTA GTACCTTCCA GGAATCTGAA 
GACAGCATTG TTCCAAAAGC CCAAGAGAAA CACACTTGTG CCACAACTCT AAACAACGAA 
GCTCAAATTA ACCTATCCTT GTTAGGGGAT GACCTAGTGG TTCCTGATCA ATCTACACTA 
GAGGCAAAGC AGTCCGAGGT CTATGGCATC ACAAACTTTG CGGAAGAATT GGCAGAGACT 
GTTGTCTCCA TGGCAACTGA AATCGCAGCA ATCTGCCTTG ACAACTCCCA TGGAAAACAG 
CCGTGGTTTT GTGCTTGGAA GAGAGGGAAC GAATTTCTGA TGACGCCTAA CGCATCCTGC 
CGATCCTTGA AGAGGAAGAA GGAAAACTCG GGCACGGGAA GCACTGTGAG GAAACACAAG 
CCACCTCGCC TCAGTGAGAT CAAGAGAAAA GCAGATGAGC ACCCAGAGCT GAAAGAGAAG 
CTGATGAACA GAGTCATGGA TGAGTCCATG AACCTCGAAG ACATTCCCGA TTCCGTCAGT 
ACTTTTGCAA ATGAAGTGGC AGCCAAGATC ATGAACCTAA CAGAATTCTC CACGGTGGAT 
GGAGTGTGGC AAACCCAGAG TTGTTCCCGG AATCGGCTTC TGGGTGGTGA TAGGTGGAAC 
CGGCTGAAGG CCTCGAGCTG TGAAAGTATT CCGGAGGAGG ACTCTGAAGC CAGGGTCTTT 
GTAAACAGCC TGGGTTTAAT GAGTACCTTA AGCCAACCCG TTAGCAGGGC CAGCTCAGTC 
TCCAAACAGT CCAGCTGTGA GAGCATCACC GATGAGTTTT CCAGGTTCAT GGTGAAACAG 
ATGGAAAACG AAGGGAGGGG GTTCGAGTTG CTGCTGGATT ACTACGCTGG CAAAAATGCC 
AGCAGTATCC TGAACTCAGC AATCCAGCAG GCATGCCAGA AAAACGACCA CCTCAACGTA 
AGACCAAGCT GCCCCTCTAA GCAGTCCAGC ACAGAAAGCA TAACAGAGGA GTTCTATAAG 
TACATGCTGA GAGACATTGC CAAAGAAAAC AAAGATGGCG CCCTGTCCAG ACGAAGCAGC 
CATGATTGGA GCACGGGCTT GCTGTCTCCT TCCGCGCGAT CCCCACTGTG TTACAGGCAG 
TCATCTATGC CTGACAGCAG GTCCCCGTGC TCCAGGTTAA CAGTAAATGC ACCTATCAAA 
GCCAACTCCT TAGATGGTTT TGCCCAAAAC TGCCCTCAAG ATTCTATAAA TGTACAGCCA 
GTCAGTAGGG CTTCCTCGTC TGGCCTCTGC AAATCAGACT CCTGTTTATA TCGCCGAAGT 
GGGACTGACC AGATCACAAA CATGCTAATT CATGAAACAT GGGCCAGCTC CATTGAGGCT 
CTCATGAGAA AGAACAAAAT CATTGCTGAT GACGGCGAGG CAGCTAACGC TAGCCCTGGT 
CCTGTTTCTG GCGGCTCGCC TTCGCAAGTA GAGAAGTGTG CCAACAGACT AGTCACCGGC 
ACAGGACACA AGGGACCAGC TCTACTTGTA CAGGAGTCTG TTGATTACCA GAGAAAGGAT 
GCTGTCACTG AAGGCAATTG TTCCCCAGTG TCATCTCCAA GCAAAATGGC TCCTGTTAAA 
AAGCCCAGTG GTTTTGATCC TACAAGAGAA ACCTCTGCAT GCCACAATGC TGTTGCTCTC 
AAAAGCCCCA GGCGGTCTCT TTGCTCAAGG GAGGTGCCTT TGATTCAGAT AGAGACCGAT 
CAGAAAGAAG AATGCGTTGG AGAATCTGAA ACCTTACTTC CCCAAAGTGG CTCCCTAGAG 
GAAGCAGAAG GGCCCCAGCC TGAGGAAACC ATCCCAGATG TGGCCAGAAG CGAAGACACA 
GCCCTGAGCG CCTGTCAAAG CTCTCATGAC AGCCTTGAGA CCAGAGAAGA ATTAGAAGTG 
GATGTCTTGA AAGAAGACAT AACCCTAGAT GAGTCCCGGA ACCCTCCTAG CAGCAGCGAA 
GAGAGTACAG GCAGCTGGTC CCAGCTGGCC AATGAGGAGG ACAACCCAGA TGACACAAGT 
AGCTTTCTGC AGCTCAGTGA GCGGTCCATG AGCAATGGCA ACACTAGTGG CACTAGCAGT 
CTTGGCATTA TGGACCTGGA CATTTATCAG GAAAGCATAC CGTCTTCTCC CATGATTAAT 
GAATTAGTAG AAGAAAAGGA GATTCTTAAA GAACAATCAG AAAGCGTAAA GGAACGTGCC 
TCTGGACTGC CAGGGAGAGC AGCCAGCCCC CAGAGGAGTC TACTGGTGAT CAACTTTGAC 
CTGGAGCCAG AGTGTCCTGA TGCTGAGCTG CGAGCCACTC TGCAGTGGAT AGCTGCCTCC 
GAGCTGGGCA TCCCAACAAT CTACTTTAAG AAATCTCAGG AAAGCAGAAT TGAAAAGTTT 
CTAGATGTTG TGAAGCTTGT TCATCAGAAG TCCTGGAAGG TGGGAGATAT ATTTCATGCC 
GTTGTCCAGT ACTGCAAAAT GCATGCAGAG CAGAAAGAGG GAACTCCGAG TCTCTTTGAC 
TGGCTCCTGG AACTAGGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of insulin signaling in skeletal muscle by PIP3 phosphatase, SKIP, and endoplasmic reticulum molecular chaperone glucose-regulated protein 78.
Biochimica et biophysica acta1853(12)3192-201(2015 Dec)
Ijuin T,Hatano N,Hosooka T,Takenawa T

Up-regulation of SKIP relates to retinal ganglion cells apoptosis after optic nerve crush in vivo.
Journal of molecular histology45(6)715-21(2014 Dec)
Wu Y,Xu F,Huang H,Chen L,Wen M,Jiang L,Lu L,Li L,Song D,Zeng S,Li L,Li M

An entirely specific type I A-kinase anchoring protein that can sequester two molecules of protein kinase A at mitochondria.
Proceedings of the National Academy of Sciences of the United States of America108(48)E1227-35(2011 Nov)
Means CK,Lygren B,Langeberg LK,Jain A,Dixon RE,Vega AL,Gold MG,Petrosyan S,Taylor SS,Murphy AN,Ha T,Santana LF,Tasken K,Scott JD

Sphingosine kinase interacting protein is an A-kinase anchoring protein specific for type I cAMP-dependent protein kinase.
Chembiochem : a European journal of chemical biology11(7)963-71(2010 May)
Kovanich D,van der Heyden MA,Aye TT,van Veen TA,Heck AJ,Scholten A

Analysis of the cGMP/cAMP interactome using a chemical proteomics approach in mammalian heart tissue validates sphingosine kinase type 1-interacting protein as a genuine and highly abundant AKAP.
Journal of proteome research5(6)1435-47(2006 Jun)
Scholten A,Poh MK,van Veen TA,van Breukelen B,Vos MA,Heck AJ

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Sphkap cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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