SPHKAP cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPHKAP
Entrez Gene ID	540759
Full Name	SPHK1 interactor, AKAP domain containing
General protein information	Preferred Names SPHK1 interactor, AKAP domain containing Names A-kinase anchor protein SPHKAP
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002685643.4	XP_002685689.2	A-kinase anchor protein SPHKAP isoform X1
XM_612993.7	XP_612993.5	A-kinase anchor protein SPHKAP isoform X1
XM_005197534.3	XP_005197591.1	A-kinase anchor protein SPHKAP isoform X2
XM_024981754.1	XP_024837522.1	A-kinase anchor protein SPHKAP isoform X1
XM_005203015.3	XP_005203072.1	A-kinase anchor protein SPHKAP isoform X2
XM_024981759.1	XP_024837527.1	A-kinase anchor protein SPHKAP isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPHKAP	XP_005635852.1
Gallus gallus (chicken)	SPHKAP	NP_001171003.1
Xenopus tropicalis (tropical clawed frog)	sphkap	XP_002933413.2
Bos taurus (cattle)	SPHKAP	XP_002685689.2
Mus musculus (house mouse)	Sphkap	NP_766018.3
Danio rerio (zebrafish)	sphkap	NP_001076514.1
Homo sapiens (human)	SPHKAP	NP_001136116.1
Pan troglodytes (chimpanzee)	SPHKAP	XP_001137841.1
	SPHKAP	XP_001111587.1
Rattus norvegicus (Norway rat)	Sphkap	NP_001120964.1

Gene Ontology

The following SPHKAP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPHKAP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa02997	XM_024981754.1 Latest version!	Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa227813	XM_024981759.1 Latest version!	Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa02995	XM_612993.7 Latest version!	Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02995	XM_002685643.4 Latest version!	Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02998	XM_005197534.3 Latest version!	Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa02998	XM_005203015.3 Latest version!	Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPHKAP ( XM_024981754.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837522.1 Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

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CloneID	OBa02997
Clone ID Related Accession (Same CDS sequence)	XM_024981754.1
Accession Version	XM_024981754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCAGG TTCCGGAAAC GCAGCAGGGC AGCGCCAGCG CCAGCTCGGG AAGTAGCCTG 
GGCGGCTCCA TCACTGCGTG TAAGAAGGTT CTCCGCAGTA ATAGCCTGCT GGAGTCTACA 
GACTACTGGC TGCAGAATCA GAGAACACCC TGCCAGATTG GTTTCGTGGA AGACAAGTCT 
GAAAACTGCG CTTCTGTCTG CTTTGTGAAC CTGGATGTGA ACAAGGATGC ATGCAGCACG 
GAGCGCCTGC AGCAGAAGTT GGTCAATGTT TCACCAGATC TTCCAAAACT TATAAGCTCC 
ATGAATGTCC AACAGCCAAA AGAAAATGAA ATTGTCCTCC TAAGTGGGTT AACATCTGGA 
AACCTACAGG CAGATTTTGA AGTTTCACAG TGTCCTTGGA TGTCAGATGT CTGCTTGGTC 
CAGTGTGCAA GAGGGAACAG ACCAAACAGT ACCAATTGCA TCATCTTTGA AATCAACAAA 
TTTTTGATTG GTCTGGAACT GGTACAGGAG CGGCAGCTCC ACCTTGAAAC AAACATCTTG 
AAAGTGGAGG ATGACACGAA CTGCTCCTTG TCTTCAATTG AAGAAGACTT TCTCACCGCA 
TCCGAGCACT TGGAGGAGGA AAGTGAAGTG GAAGAGTATA GGAACGGTTA TGAAAATATA 
AATGTCTCAA CCAGTATCTT GGAAAATAAA AAGCCAAAGG AAGCCACTAA GGAGGAATGG 
AATTACAGCA AGGAAAAATT ACTTTATACA TTGGAAGACA AGTATGTTAG CAAATATCAT 
CCACCACTGG TTAAAACAGA AGGATCTCTG GGAAGAGTGG CAGAATATAC AAGTCTGCAG 
AGTCTAGATC CTTCAGCCAA GATATCAAAG TGGAAAGGTG AAGCTGTGGG GAATGAGCGG 
TCAACCACAA AGGATTATTC TTCAGAAAAG TGTAAAAGTC AAGAGGAAAA CTCACAGGCG 
CTATGTAGTC CAGGCAATGC TTATTTCTCA AAGATGGTTA AGAGGGATGG ACTTTCTGCA 
TGTGGGACTT TCTCTGAGCG TGACAGCAAT TTAGACCCAG GAGACCGTGA AGACCGCACA 
AGCTCTCTTC TTCCCTTACA AGATGGAGAA GCCACAACAG GCGAGTATGC CACGAATTTA 
GCGGAATCTG TGATGCAAGA TGCATTTCTT CGACTCTGTA AGTCTCAGCC TGCCCTCCCC 
CAGGAATCTG CAGTCAGTGT TTCTGTAGGA AGTGCTCTGC TTCCCAGTGG CTCTTCCACA 
AAAGATATCG TGGTACCTCA GTCATGGAAC GAGCCCCCCA AAATCGTCAT TGTCCAGAGT 
CCAGAAGGCA GTGATGCTGG CCCTGAGCCA GGCATCTCCT CGTGGCCCGA GACAGAAGTC 
TCCAGTGAAA CTTCAGGTGT CCCCTCTGGA GAGAACTCCA GAAGACGCCC CCAGAGTCCT 
CTAGAAGTAG CATTAGCTTG TGCAGCCACT GTTATTGGGA CCATTTCCAG TCCACAAGCC 
ACAGAAAGAT TCAAAATGGA GCAAGAACCT CTGGTTTCAC ACCATCCACT GGGAGGTGAT 
GAGCCAGGAC AAACTCAGGC CTCTCAAGTA CTCAAGGATC CCTCCCTCAG TGAATACTCG 
TTTCCGTCTG CTTTATGTGG CATGACTCAG GTGGCCAGTG CTGTTGCTGT CTGTGGCCTG 
GGAGAAACAG GAGAGGTGAA GTGCCCGGTG GCTTCCAGTG GCTTCCTGTC TGAAGTTGAG 
GCCCCTGCAG CCATCCCCCC ACTTTGTGGT TTAGTGGCAG AAAAGAGCAT GGAGCTGGGG 
AATGAAGCCA TTGCAGAGGC CTTGCTCAAG GAGGTTGCCT TGGTCTTAAC AAGACCTAAC 
GCCTACAGCA GCATTGGAGA CCTCATGGAG TCAGTGAATA GGAGGATCGT AGAAGCTGTT 
TCAAAGCCTC AGACCTTGTG CTCAGAAAAC GCCCTTGGGA ATGAACTGGC ACAGACCCTG 
TCTAATGTTA TCCTGAAGCA TTCCATTGAT GAAATTCACC AGAAAAATAA AATGATAGAC 
CCCAATGATG ACAGGGACTC ATCTGAAACC CTGGAATCCT TAATGGAAAG TGTAAATCAG 
CTGCTCTTCA ATGTGATACG CTTCACGTTC GAGAAGATGA GCCACCTTGT ACAGCTTGGG 
GGATGTCCCC CTGTCCTTTC TAAGGAGACC ATCAGATGGA GGGAAACCGA ACCAGACTTC 
CAGCCAACTG ATCAAGCCAT TTGTCCAGCA TGGACAAAAG CCGCTGAATA CTCCAATTGC 
CATCCACTTA GCAACCTGCA TATCAGCAGT CCTGTTATCA ATGATCTTGT AGGTGCTGGA 
CATCCGAAGC AAGATAGCAA GGGTCCAGCA GAGTCAGGTC TTTCCCAGAT CCCCACACTG 
CAATCTGAAT TATCCTGCAG TACCAGCATG TCTGCTAAAA CACCACCCAA GGAAATATAT 
CTGAAAGGAT CGGTGGTGGA GGATATGAAA AACCCTCCTG CACCACCCAG TGATAATGAG 
AATGAATGCA GAGCCCCTCC AGAGGAAGAA ATGACACCAA CAGTTGGCAA GTACAGCATC 
ATCTCTCAGG ACACTGAGGA CAGCATCATT GCAAATGCTC AAGAAAAGTA CAATGGTGCC 
TTGCCTCTGA ACAGTGAACT TCAAGTTTGC CCGTCTTTGT TGGGGGATGA TTTGCTGCTT 
CCTGCTCAAT CCACGCTACC AGAAAAAGAC CCAGACATAT ACTGCATTGC AGACTTCGCA 
GAAGAATTAG CAGAGACGAT CGTCTCCATG GCAACGGAAA TCGCAGCGAT TTGCCTTGAC 
AACTCCAATG GAAAACAACC CTGGTTCTGT GCATGGAAAA GAGGGAATGA GTTTCTGGTT 
ACCCCCAACA TCTCCTGCCG ATCCTTGAAG AAGAAGAAAG AGAGCCAGGC CAGCGGGGCC 
ACTGTGAGGA AACACAAGCC CCCGCGACTC AGCGAGATCA AGAGGAAAAC CGATGAGCAC 
CCGGAGCTGA AAGAGAAGCT GATGAACAGG GTGGTGGACG AGTCCATGAA CCTCGAGGAC 
ATCCCAGACT CTGTCAGTAT TTTTGCAAAC GAAGTGGCAG CCAAGATCAT GAACCTCACA 
GAGTTCTCCT TGGTGGACGG TGTGTGGCAA GCACAGGGCT ACCCCCGGAG TCGGTTACTG 
GGTGGGGACA GGTGGAACCG CCTGAAGGCC TCCAGCTGCG AGAGCATTCC AGAGGAGGAC 
TCTAACACCC GGGCCTATGT GAACAGCCTG GGTTTAATGA GTACCCTGAG TCAGCCAGTT 
AGCCGGGCCA GCTCCGTCTC TAAGCAGTCG AGCTGTGAGA GCATCACCGA TGAGTTTTCC 
AGGTTCATGG TGAACCAGAT GGAAAATGAA GGAAGAGGCT TCGAGTTACT GCTGGATTAC 
TACGCAGGCA AGAATGCCAG CAGCATCTTG AACTCAGCTG TGCAACAGGC CTGCCGGAAA 
AACGACCATC TGAGTGTGAA GCCAAGCTGT CCCTCGAAGC AGTCCAGCAC CGAAAGCATC 
ACCGAGGAGT TCTACAGGTA CATGCTGAGG GACATTGAAC GAGAGGGCAA AGACGGCCCC 
TCCTCCAGGC AAGGCAGCCA GGACTGGACC ACTGGCTTGC TCTCGCCTTC TCTGCGGTCC 
CCCTTGTACT ACAGACAGTC GTCTGTGCCC GACGGCAGAT CTCCGGGCGC CAGGCTCACG 
GTGAACGTGC CCATCAAAGC TAACTCCTTA GACGGCTTTT CTCAACACAG CCAGCAAGAC 
TTCCTCAGCG TCCAGCCAGT CGGCGGCGCT TCCTCCTCGG GTCTCTGCAA ATCTGACTCT 
TGCCTGTATC GCAGAGGCGG GGCTGACCAC ATCACAAACA TGTTAATTCA CGAGACGTGG 
GCAAACTCAA TCGAGGCTCT CATGCGGAAG AACAAAATTA TAGTGGATGA TGCCGGCGCG 
GCTGATGCTG AACCTCCTTC AGGGGGCCCC TCCTTGCAAG TGGAAAAGTG TGCGGGCAGA 
TTAGCTTCGG GCAGGGTGCA AAGTGGACCA GCTCTGCTTG TGCAGGAGTC TGTGGATTAT 
CCGAGGAAAA ACTCAATCAC AGAAAGCAGC CATCTCCCAG GGTCGTCCCC AAGCAAAGCT 
GCTCCTCTTA CAAGTCACAG CAGTTTAGAT TTTAAAAAGG AAACTCCCTC ATGCCACCAT 
GCGGTCCCTC TAAGCCACAC CCGGCGACCA CTTTGCTCAA GGGAAGTGCC TTTAATCCAG 
ATCGAAACAG ATCAGAGAGA AGAATGTGTG GGAGAACCTG AACACGTTAT TTCCGAACAC 
AGCCCCCTAG AGGAAAAAGA AAGGTGTTTG GAAGAAGAAA AGGTCCCAGA GTCAGGGAAG 
GGTGAAGACG CTGCCCTGAG TGCCTGCCGG AACCCCAGTG AAACCCTTGA TGCCAGAGAT 
GTACCGGAGG CTGAAGTCTC AAAAGAAGGC AGAGCCCTGG ATGAGTTCTC CAGCCCTCCT 
GGCAGCAGTG GGGAGAGCAT GGACAGTTGG TCCCAGCTCG CCAACGAGGA GGACAACCCA 
GACGACACAA GCAGCTTCCT GCAGCTCAGT GAGCGATCCA TGAGCAATGG CAACAGTAGT 
GCCACTAGCA GTCTTGGCAT TATGGACCTG GACATTTATC AGGAAAGCAT GCCATCTTCT 
CCCATGATTA ATGAATTGGT AGAAGAAAAG GAGATTCTTA AAGGACAGGC AGAAACCATG 
GAGGAACGTG CCTCTGGAAC GCCGGTTGGA ACAGCCAGCC GCCAGGGGAG CCTGCTGGTG 
ATCAACTTTG ACCTGGAGCC AGAGTGTCCT GACACGGAGC TGCGAGCCAC TCTGCAGTGG 
ATAGCTGCAT CTGAACTCGG TATCCCCACC ATCTACTTTA AGAAATCTCA AGAAAACAGA 
ATCGAAAAGT TTCTAGATGT CGTGCGGCTG GTCCATCAGA AGTCCTGGAA GGTGGGGGAT 
ATCTTCCATG CAGTGGTTCA GTACTGCAAG ATACATGAGG AACGGAGGGA TGGGACCCCG 
AGTCTCTTTG ATTGGCTCTT GGAACTGGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837522.1
CDS	163..5235
Translation	MYQVPETQQGSASASSGSSLGGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDKSENCASVCFVNLDVNKDACSTERLQQKLVNVSPDLPKLISSMNVQQPKENEIVLLSGLTSGNLQADFEVSQCPWMSDVCLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKVEDDTNCSLSSIEEDFLTASEHLEEESEVEEYRNGYENINVSTSILENKKPKEATKEEWNYSKEKLLYTLEDKYVSKYHPPLVKTEGSLGRVAEYTSLQSLDPSAKISKWKGEAVGNERSTTKDYSSEKCKSQEENSQALCSPGNAYFSKMVKRDGLSACGTFSERDSNLDPGDREDRTSSLLPLQDGEATTGEYATNLAESVMQDAFLRLCKSQPALPQESAVSVSVGSALLPSGSSTKDIVVPQSWNEPPKIVIVQSPEGSDAGPEPGISSWPETEVSSETSGVPSGENSRRRPQSPLEVALACAATVIGTISSPQATERFKMEQEPLVSHHPLGGDEPGQTQASQVLKDPSLSEYSFPSALCGMTQVASAVAVCGLGETGEVKCPVASSGFLSEVEAPAAIPPLCGLVAEKSMELGNEAIAEALLKEVALVLTRPNAYSSIGDLMESVNRRIVEAVSKPQTLCSENALGNELAQTLSNVILKHSIDEIHQKNKMIDPNDDRDSSETLESLMESVNQLLFNVIRFTFEKMSHLVQLGGCPPVLSKETIRWRETEPDFQPTDQAICPAWTKAAEYSNCHPLSNLHISSPVINDLVGAGHPKQDSKGPAESGLSQIPTLQSELSCSTSMSAKTPPKEIYLKGSVVEDMKNPPAPPSDNENECRAPPEEEMTPTVGKYSIISQDTEDSIIANAQEKYNGALPLNSELQVCPSLLGDDLLLPAQSTLPEKDPDIYCIADFAEELAETIVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLVTPNISCRSLKKKKESQASGATVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDIPDSVSIFANEVAAKIMNLTEFSLVDGVWQAQGYPRSRLLGGDRWNRLKASSCESIPEEDSNTRAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAVQQACRKNDHLSVKPSCPSKQSSTESITEEFYRYMLRDIEREGKDGPSSRQGSQDWTTGLLSPSLRSPLYYRQSSVPDGRSPGARLTVNVPIKANSLDGFSQHSQQDFLSVQPVGGASSSGLCKSDSCLYRRGGADHITNMLIHETWANSIEALMRKNKIIVDDAGAADAEPPSGGPSLQVEKCAGRLASGRVQSGPALLVQESVDYPRKNSITESSHLPGSSPSKAAPLTSHSSLDFKKETPSCHHAVPLSHTRRPLCSREVPLIQIETDQREECVGEPEHVISEHSPLEEKERCLEEEKVPESGKGEDAALSACRNPSETLDARDVPEAEVSKEGRALDEFSSPPGSSGESMDSWSQLANEEDNPDDTSSFLQLSERSMSNGNSSATSSLGIMDLDIYQESMPSSPMINELVEEKEILKGQAETMEERASGTPVGTASRQGSLLVINFDLEPECPDTELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVRLVHQKSWKVGDIFHAVVQYCKIHEERRDGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_024981754.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981754.1

ORF Insert Sequence:

ATGTACCAGG TTCCGGAAAC GCAGCAGGGC AGCGCCAGCG CCAGCTCGGG AAGTAGCCTG 
GGCGGCTCCA TCACTGCGTG TAAGAAGGTT CTCCGCAGTA ATAGCCTGCT GGAGTCTACA 
GACTACTGGC TGCAGAATCA GAGAACACCC TGCCAGATTG GTTTCGTGGA AGACAAGTCT 
GAAAACTGCG CTTCTGTCTG CTTTGTGAAC CTGGATGTGA ACAAGGATGC ATGCAGCACG 
GAGCGCCTGC AGCAGAAGTT GGTCAATGTT TCACCAGATC TTCCAAAACT TATAAGCTCC 
ATGAATGTCC AACAGCCAAA AGAAAATGAA ATTGTCCTCC TAAGTGGGTT AACATCTGGA 
AACCTACAGG CAGATTTTGA AGTTTCACAG TGTCCTTGGA TGTCAGATGT CTGCTTGGTC 
CAGTGTGCAA GAGGGAACAG ACCAAACAGT ACCAATTGCA TCATCTTTGA AATCAACAAA 
TTTTTGATTG GTCTGGAACT GGTACAGGAG CGGCAGCTCC ACCTTGAAAC AAACATCTTG 
AAAGTGGAGG ATGACACGAA CTGCTCCTTG TCTTCAATTG AAGAAGACTT TCTCACCGCA 
TCCGAGCACT TGGAGGAGGA AAGTGAAGTG GAAGAGTATA GGAACGGTTA TGAAAATATA 
AATGTCTCAA CCAGTATCTT GGAAAATAAA AAGCCAAAGG AAGCCACTAA GGAGGAATGG 
AATTACAGCA AGGAAAAATT ACTTTATACA TTGGAAGACA AGTATGTTAG CAAATATCAT 
CCACCACTGG TTAAAACAGA AGGATCTCTG GGAAGAGTGG CAGAATATAC AAGTCTGCAG 
AGTCTAGATC CTTCAGCCAA GATATCAAAG TGGAAAGGTG AAGCTGTGGG GAATGAGCGG 
TCAACCACAA AGGATTATTC TTCAGAAAAG TGTAAAAGTC AAGAGGAAAA CTCACAGGCG 
CTATGTAGTC CAGGCAATGC TTATTTCTCA AAGATGGTTA AGAGGGATGG ACTTTCTGCA 
TGTGGGACTT TCTCTGAGCG TGACAGCAAT TTAGACCCAG GAGACCGTGA AGACCGCACA 
AGCTCTCTTC TTCCCTTACA AGATGGAGAA GCCACAACAG GCGAGTATGC CACGAATTTA 
GCGGAATCTG TGATGCAAGA TGCATTTCTT CGACTCTGTA AGTCTCAGCC TGCCCTCCCC 
CAGGAATCTG CAGTCAGTGT TTCTGTAGGA AGTGCTCTGC TTCCCAGTGG CTCTTCCACA 
AAAGATATCG TGGTACCTCA GTCATGGAAC GAGCCCCCCA AAATCGTCAT TGTCCAGAGT 
CCAGAAGGCA GTGATGCTGG CCCTGAGCCA GGCATCTCCT CGTGGCCCGA GACAGAAGTC 
TCCAGTGAAA CTTCAGGTGT CCCCTCTGGA GAGAACTCCA GAAGACGCCC CCAGAGTCCT 
CTAGAAGTAG CATTAGCTTG TGCAGCCACT GTTATTGGGA CCATTTCCAG TCCACAAGCC 
ACAGAAAGAT TCAAAATGGA GCAAGAACCT CTGGTTTCAC ACCATCCACT GGGAGGTGAT 
GAGCCAGGAC AAACTCAGGC CTCTCAAGTA CTCAAGGATC CCTCCCTCAG TGAATACTCG 
TTTCCGTCTG CTTTATGTGG CATGACTCAG GTGGCCAGTG CTGTTGCTGT CTGTGGCCTG 
GGAGAAACAG GAGAGGTGAA GTGCCCGGTG GCTTCCAGTG GCTTCCTGTC TGAAGTTGAG 
GCCCCTGCAG CCATCCCCCC ACTTTGTGGT TTAGTGGCAG AAAAGAGCAT GGAGCTGGGG 
AATGAAGCCA TTGCAGAGGC CTTGCTCAAG GAGGTTGCCT TGGTCTTAAC AAGACCTAAC 
GCCTACAGCA GCATTGGAGA CCTCATGGAG TCAGTGAATA GGAGGATCGT AGAAGCTGTT 
TCAAAGCCTC AGACCTTGTG CTCAGAAAAC GCCCTTGGGA ATGAACTGGC ACAGACCCTG 
TCTAATGTTA TCCTGAAGCA TTCCATTGAT GAAATTCACC AGAAAAATAA AATGATAGAC 
CCCAATGATG ACAGGGACTC ATCTGAAACC CTGGAATCCT TAATGGAAAG TGTAAATCAG 
CTGCTCTTCA ATGTGATACG CTTCACGTTC GAGAAGATGA GCCACCTTGT ACAGCTTGGG 
GGATGTCCCC CTGTCCTTTC TAAGGAGACC ATCAGATGGA GGGAAACCGA ACCAGACTTC 
CAGCCAACTG ATCAAGCCAT TTGTCCAGCA TGGACAAAAG CCGCTGAATA CTCCAATTGC 
CATCCACTTA GCAACCTGCA TATCAGCAGT CCTGTTATCA ATGATCTTGT AGGTGCTGGA 
CATCCGAAGC AAGATAGCAA GGGTCCAGCA GAGTCAGGTC TTTCCCAGAT CCCCACACTG 
CAATCTGAAT TATCCTGCAG TACCAGCATG TCTGCTAAAA CACCACCCAA GGAAATATAT 
CTGAAAGGAT CGGTGGTGGA GGATATGAAA AACCCTCCTG CACCACCCAG TGATAATGAG 
AATGAATGCA GAGCCCCTCC AGAGGAAGAA ATGACACCAA CAGTTGGCAA GTACAGCATC 
ATCTCTCAGG ACACTGAGGA CAGCATCATT GCAAATGCTC AAGAAAAGTA CAATGGTGCC 
TTGCCTCTGA ACAGTGAACT TCAAGTTTGC CCGTCTTTGT TGGGGGATGA TTTGCTGCTT 
CCTGCTCAAT CCACGCTACC AGAAAAAGAC CCAGACATAT ACTGCATTGC AGACTTCGCA 
GAAGAATTAG CAGAGACGAT CGTCTCCATG GCAACGGAAA TCGCAGCGAT TTGCCTTGAC 
AACTCCAATG GAAAACAACC CTGGTTCTGT GCATGGAAAA GAGGGAATGA GTTTCTGGTT 
ACCCCCAACA TCTCCTGCCG ATCCTTGAAG AAGAAGAAAG AGAGCCAGGC CAGCGGGGCC 
ACTGTGAGGA AACACAAGCC CCCGCGACTC AGCGAGATCA AGAGGAAAAC CGATGAGCAC 
CCGGAGCTGA AAGAGAAGCT GATGAACAGG GTGGTGGACG AGTCCATGAA CCTCGAGGAC 
ATCCCAGACT CTGTCAGTAT TTTTGCAAAC GAAGTGGCAG CCAAGATCAT GAACCTCACA 
GAGTTCTCCT TGGTGGACGG TGTGTGGCAA GCACAGGGCT ACCCCCGGAG TCGGTTACTG 
GGTGGGGACA GGTGGAACCG CCTGAAGGCC TCCAGCTGCG AGAGCATTCC AGAGGAGGAC 
TCTAACACCC GGGCCTATGT GAACAGCCTG GGTTTAATGA GTACCCTGAG TCAGCCAGTT 
AGCCGGGCCA GCTCCGTCTC TAAGCAGTCG AGCTGTGAGA GCATCACCGA TGAGTTTTCC 
AGGTTCATGG TGAACCAGAT GGAAAATGAA GGAAGAGGCT TCGAGTTACT GCTGGATTAC 
TACGCAGGCA AGAATGCCAG CAGCATCTTG AACTCAGCTG TGCAACAGGC CTGCCGGAAA 
AACGACCATC TGAGTGTGAA GCCAAGCTGT CCCTCGAAGC AGTCCAGCAC CGAAAGCATC 
ACCGAGGAGT TCTACAGGTA CATGCTGAGG GACATTGAAC GAGAGGGCAA AGACGGCCCC 
TCCTCCAGGC AAGGCAGCCA GGACTGGACC ACTGGCTTGC TCTCGCCTTC TCTGCGGTCC 
CCCTTGTACT ACAGACAGTC GTCTGTGCCC GACGGCAGAT CTCCGGGCGC CAGGCTCACG 
GTGAACGTGC CCATCAAAGC TAACTCCTTA GACGGCTTTT CTCAACACAG CCAGCAAGAC 
TTCCTCAGCG TCCAGCCAGT CGGCGGCGCT TCCTCCTCGG GTCTCTGCAA ATCTGACTCT 
TGCCTGTATC GCAGAGGCGG GGCTGACCAC ATCACAAACA TGTTAATTCA CGAGACGTGG 
GCAAACTCAA TCGAGGCTCT CATGCGGAAG AACAAAATTA TAGTGGATGA TGCCGGCGCG 
GCTGATGCTG AACCTCCTTC AGGGGGCCCC TCCTTGCAAG TGGAAAAGTG TGCGGGCAGA 
TTAGCTTCGG GCAGGGTGCA AAGTGGACCA GCTCTGCTTG TGCAGGAGTC TGTGGATTAT 
CCGAGGAAAA ACTCAATCAC AGAAAGCAGC CATCTCCCAG GGTCGTCCCC AAGCAAAGCT 
GCTCCTCTTA CAAGTCACAG CAGTTTAGAT TTTAAAAAGG AAACTCCCTC ATGCCACCAT 
GCGGTCCCTC TAAGCCACAC CCGGCGACCA CTTTGCTCAA GGGAAGTGCC TTTAATCCAG 
ATCGAAACAG ATCAGAGAGA AGAATGTGTG GGAGAACCTG AACACGTTAT TTCCGAACAC 
AGCCCCCTAG AGGAAAAAGA AAGGTGTTTG GAAGAAGAAA AGGTCCCAGA GTCAGGGAAG 
GGTGAAGACG CTGCCCTGAG TGCCTGCCGG AACCCCAGTG AAACCCTTGA TGCCAGAGAT 
GTACCGGAGG CTGAAGTCTC AAAAGAAGGC AGAGCCCTGG ATGAGTTCTC CAGCCCTCCT 
GGCAGCAGTG GGGAGAGCAT GGACAGTTGG TCCCAGCTCG CCAACGAGGA GGACAACCCA 
GACGACACAA GCAGCTTCCT GCAGCTCAGT GAGCGATCCA TGAGCAATGG CAACAGTAGT 
GCCACTAGCA GTCTTGGCAT TATGGACCTG GACATTTATC AGGAAAGCAT GCCATCTTCT 
CCCATGATTA ATGAATTGGT AGAAGAAAAG GAGATTCTTA AAGGACAGGC AGAAACCATG 
GAGGAACGTG CCTCTGGAAC GCCGGTTGGA ACAGCCAGCC GCCAGGGGAG CCTGCTGGTG 
ATCAACTTTG ACCTGGAGCC AGAGTGTCCT GACACGGAGC TGCGAGCCAC TCTGCAGTGG 
ATAGCTGCAT CTGAACTCGG TATCCCCACC ATCTACTTTA AGAAATCTCA AGAAAACAGA 
ATCGAAAAGT TTCTAGATGT CGTGCGGCTG GTCCATCAGA AGTCCTGGAA GGTGGGGGAT 
ATCTTCCATG CAGTGGTTCA GTACTGCAAG ATACATGAGG AACGGAGGGA TGGGACCCCG 
AGTCTCTTTG ATTGGCTCTT GGAACTGGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_024981759.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837527.1 Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

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CloneID	OBa227813
Clone ID Related Accession (Same CDS sequence)	XM_024981759.1
Accession Version	XM_024981759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCAGG TTCCGGAAAC GCAGCAGGGC AGCGCCAGCG CCAGCTCGGG AAGTAGCCTG 
GGCGGCTCCA TCACTGCGTG TAAGAAGGTT CTCCGCAGTA ATAGCCTGCT GGAGTCTACA 
GACTACTGGC TGCAGAATCA GAGAACACCC TGCCAGATTG GTTTCGTGGA AGACAAGTCT 
GAAAACTGCG CTTCTGTCTG CTTTGTGAAC CTGGATGTGA ACAAGGATGC ATGCAGCACG 
GAGCGCCTGC AGCAGAAGTT GGTCAATGTT TCACCAGATC TTCCAAAACT TATAAGCTCC 
ATGAATGTCC AACAGCCAAA AGAAAATGAA ATTGTCCTCC TAAGTGGGTT AACATCTGGA 
AACCTACAGG CAGATTTTGA AGTTTCACAG TGTCCTTGGA TGTCAGATGT CTGCTTGGTC 
CAGTGTGCAA GAGGGAACAG ACCAAACAGT ACCAATTGCA TCATCTTTGA AATCAACAAA 
TTTTTGATTG GTCTGGAACT GGTACAGGAG CGGCAGCTCC ACCTTGAAAC AAACATCTTG 
AAAGTGGAGG ATGACACGAA CTGCTCCTTG TCTTCAATTG AAGAAGACTT TCTCACCGCA 
TCCGAGCACT TGGAGGAGGA AAGTGAAGTG GAAGAGTATA GGAACGGTTA TGAAAATATA 
AATGTCTCAA CCAGTATCTT GGAAAATAAA AAGCCAAAGG AAGCCACTAA GGAGGAATGG 
AATTACAGCA AGGAAAAATT ACTTTATACA TTGGAAGACA AGTATGTTAG CAAATATCAT 
CCACCACTGG TTAAAACAGA AGGATCTCTG GGAAGAGTGG CAGAATATAC AAGTCTGCAG 
AGTCTAGATC CTTCAGCCAA GATATCAAAG TGGAAAGGTG AAGCTGTGGG GAATGAGCGG 
TCAACCACAA AGGATTATTC TTCAGAAAAG TGTAAAAGTC AAGAGGAAAA CTCACAGGCG 
CTATGTAGTC CAGGCAATGC TTATTTCTCA AAGATGGTTA AGAGGGATGG ACTTTCTGCA 
TGTGGGACTT TCTCTGAGCG TGACAGCAAT TTAGACCCAG GAGACCGTGA AGACCGCACA 
AGCTCTCTTC TTCCCTTACA AGATGGAGAA GCCACAACAG GCGAGTATGC CACGAATTTA 
GCGGAATCTG TGATGCAAGA TGCATTTCTT CGACTCTGTA AGTCTCAGCC TGCCCTCCCC 
CAGGAATCTG CAGTCAGTGT TTCTGTAGGA AGTGCTCTGC TTCCCAGTGG CTCTTCCACA 
AAAGATATCG TGGTACCTCA GTCATGGAAC GAGCCCCCCA AAATCGTCAT TGTCCAGAGT 
CCAGAAGGCA GTGATGCTGG CCCTGAGCCA GGCATCTCCT CGTGGCCCGA GACAGAAGTC 
TCCAGTGAAA CTTCAGGTGT CCCCTCTGGA GAGAACTCCA GAAGACGCCC CCAGAGTCCT 
CTAGAAGTAG CATTAGCTTG TGCAGCCACT GTTATTGGGA CCATTTCCAG TCCACAAGCC 
ACAGAAAGAT TCAAAATGGA GCAAGAACCT CTGGTTTCAC ACCATCCACT GGGAGGTGAT 
GAGCCAGGAC AAACTCAGGC CTCTCAAGTA CTCAAGGATC CCTCCCTCAG TGAATACTCG 
TTTCCGTCTG CTTTATGTGG CATGACTCAG GTGGCCAGTG CTGTTGCTGT CTGTGGCCTG 
GGAGAAACAG GAGAGGTGAA GTGCCCGGTG GCTTCCAGTG GCTTCCTGTC TGAAGTTGAG 
GCCCCTGCAG CCATCCCCCC ACTTTGTGGT TTAGTGGCAG AAAAGAGCAT GGAGCTGGGG 
AATGAAGCCA TTGCAGAGGC CTTGCTCAAG GAGGTTGCCT TGGTCTTAAC AAGACCTAAC 
GCCTACAGCA GCATTGGAGA CCTCATGGAG TCAGTGAATA GGAGGATCGT AGAAGCTGTT 
TCAAAGCCTC AGACCTTGTG CTCAGAAAAC GCCCTTGGGA ATGAACTGGC ACAGACCCTG 
TCTAATGTTA TCCTGAAGCA TTCCATTGAT GAAATTCACC AGAAAAATAA AATGATAGAC 
CCCAATGATG ACAGGGACTC ATCTGAAACC CTGGAATCCT TAATGGAAAG TGTAAATCAG 
CTGCTCTTCA ATGTGATACG CTTCACGTTC GAGAAGATGA GCCACCTTGT ACAGCTTGGG 
GGATGTCCCC CTGTCCTTTC TAAGGAGACC ATCAGATGGA GGGAAACCGA ACCAGACTTC 
CAGCCAACTG ATCAAGCCAT TTGTCCAGCA TGGACAAAAG CCGCTGAATA CTCCAATTGC 
CATCCACTTA GCAACCTGCA TATCAGCAGT CCTGTTATCA ATGATCTTGT AGGTGCTGGA 
CATCCGAAGC AAGATAGCAA GGGTCCAGCA GAGTCAGGTC TTTCCCAGAT CCCCACACTG 
CAATCTGAAT TATCCTGCAG TACCAGCATG TCTGCTAAAA CACCACCCAA GGAAATATAT 
CTGAAAGGAT CGGTGGTGGA GGATATGAAA AACCCTCCTG CACCACCCAG TGATAATGAG 
AATGAATGCA GAGCCCCTCC AGAGGAAGAA ATGACACCAA CAGTTGGCAA GTACAGCATC 
ATCTCTCAGG ACACTGAGGA CAGCATCATT GCAAATGCTC AAGAAAAGTA CAATGGTGCC 
TTGCCTCTGA ACAGTGAACT TCAAGTTTGC CCGTCTTTGT TGGGGGATGA TTTGCTGCTT 
CCTGCTCAAT CCACGCTACC AGAAAAAGAC CCAGACATAT ACTGCATTGC AGACTTCGCA 
GAAGAATTAG CAGAGACGAT CGTCTCCATG GCAACGGAAA TCGCAGCGAT TTGCCTTGAC 
AACTCCAATG GAAAACAACC CTGGTTCTGT GCATGGAAAA GAGGGAATGA GTTTCTGGTT 
ACCCCCAACA TCTCCTGCCG ATCCTTGAAG AAGAAGAAAG AGAGCCAGGC CAGCGGGGCC 
ACTGTGAGGA AACACAAGCC CCCGCGACTC AGCGAGATCA AGAGGAAAAC CGATGAGCAC 
CCGGAGCTGA AAGAGAAGCT GATGAACAGG GTGGTGGACG AGTCCATGAA CCTCGAGGAC 
ATCCCAGACT CTGTCAGTAT TTTTGCAAAC GAAGTGGCAG CCAAGATCAT GAACCTCACA 
GAGTTCTCCT TGGTGGACGG TGTGTGGCAA GCACAGGGCT ACCCCCGGAG TCGGTTACTG 
GGTGGGGACA GGTGGAACCG CCTGAAGGCC TCCAGCTGCG AGAGCATTCC AGAGGAGGAC 
TCTAACACCC GGGCCTATGT GAACAGCCTG GGTTTAATGA GTACCCTGAG TCAGCCAGTT 
AGCCGGGCCA GCTCCGTCTC TAAGCAGTCG AGCTGTGAGA GCATCACCGA TGAGTTTTCC 
AGGTTCATGG TGAACCAGAT GGAAAATGAA GGAAGAGGCT TCGAGTTACT GCTGGATTAC 
TACGCAGGCA AGAATGCCAG CAGCATCTTG AACTCAGCTG TGCAACAGGC CTGCCGGAAA 
AACGACCATC TGAGTGTGAA GCCAAGCTGT CCCTCGAAGC AGTCCAGCAC CGAAAGCATC 
ACCGAGGAGT TCTACAGGTA CATGCTGAGG GACATTGAAC GAGAGGGCAA AGACGGCCCC 
TCCTCCAGGC AAGGCAGCCA GGACTGGACC ACTGGCTTGC TCTCGCCTTC TCTGCGGTCC 
CCCTTGTACT ACAGACAGTC GTCTGTGCCC GACGGCAGAT CTCCGGGCGC CAGGCTCACG 
GTGAACGTGC CCATCAAAGC TAACTCCTTA GACGGCTTTT CTCAACACAG CCAGCAAGAC 
TTCCTCAGCG TCCAGCCAGT CGGCGGCGCT TCCTCCTCGG GTCTCTGCAA ATCTGACTCT 
TGCCTGTATC GCAGAGGCGG GGCTGACCAC ATCACAAACA TGTTAATTCA CGAGACGTGG 
GCAAACTCAA TCGAGGCTCT CATGCGGAAG AACAAAATTA TAGTGGATGA TGCCGGCGCG 
GCTGATGCTG AACCTCCTTC AGGGGGCCCC TCCTTGCAAG TGGAAAAGTG TGCGGGCAGA 
TTAGCTTCGG GCAGGGTGCA AAGTGGACCA GCTCTGCTTG TGCAGGAGTC TGTGGATTAT 
CCGAGGAAAA ACTCAATCAC AGAAAGCAGC CATCTCCCAG GGTCGTCCCC AAGCAAAGCT 
GCTCCTCTTA CAAGTCACAG CAGTTTAGAT TTTAAAAAGG AAACTCCCTC ATGCCACCAT 
GCGGTCCCTC TAAGCCACAC CCGGCGACCA CTTTGCTCAA GGGAAGTGCC TTTAATCCAG 
ATCGAAACAG ATCAGAGAGA AGAATGTGTG GGAGAACCTG AACACGTTAT TTCCGAACAC 
AGCCCCCTAG AGGAAAAAGA AAGGTGTTTG GAAGAAGAAA AGGTCCCAGA GTCAGGGAAG 
GGTGAAGACG CTGCCCTGAG TGCCTGCCGG AACCCCAGTG AAACCCTTGA TGCCAGAGAT 
GTACCGGAGG CTGAAGTCTC AAAAGAAGGC AGAGCCCTGG ATGAGTTCTC CAGCCCTCCT 
GGCAGCAGTG GGGAGAGCAT GGACAGTTGG TCCCAGCTCG CCAACGAGGA GGACAACCCA 
GACGACACAA GCAGCTTCCT GCAGCTCAGT GAGCGATCCA TGAGTGAATT GGTAGAAGAA 
AAGGAGATTC TTAAAGGACA GGCAGAAACC ATGGAGGAAC GTGCCTCTGG AACGCCGGTT 
GGAACAGCCA GCCGCCAGGG GAGCCTGCTG GTGATCAACT TTGACCTGGA GCCAGAGTGT 
CCTGACACGG AGCTGCGAGC CACTCTGCAG TGGATAGCTG CATCTGAACT CGGTATCCCC 
ACCATCTACT TTAAGAAATC TCAAGAAAAC AGAATCGAAA AGTTTCTAGA TGTCGTGCGG 
CTGGTCCATC AGAAGTCCTG GAAGGTGGGG GATATCTTCC ATGCAGTGGT TCAGTACTGC 
AAGATACATG AGGAACGGAG GGATGGGACC CCGAGTCTCT TTGATTGGCT CTTGGAACTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837527.1
CDS	163..5148
Translation	MYQVPETQQGSASASSGSSLGGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDKSENCASVCFVNLDVNKDACSTERLQQKLVNVSPDLPKLISSMNVQQPKENEIVLLSGLTSGNLQADFEVSQCPWMSDVCLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKVEDDTNCSLSSIEEDFLTASEHLEEESEVEEYRNGYENINVSTSILENKKPKEATKEEWNYSKEKLLYTLEDKYVSKYHPPLVKTEGSLGRVAEYTSLQSLDPSAKISKWKGEAVGNERSTTKDYSSEKCKSQEENSQALCSPGNAYFSKMVKRDGLSACGTFSERDSNLDPGDREDRTSSLLPLQDGEATTGEYATNLAESVMQDAFLRLCKSQPALPQESAVSVSVGSALLPSGSSTKDIVVPQSWNEPPKIVIVQSPEGSDAGPEPGISSWPETEVSSETSGVPSGENSRRRPQSPLEVALACAATVIGTISSPQATERFKMEQEPLVSHHPLGGDEPGQTQASQVLKDPSLSEYSFPSALCGMTQVASAVAVCGLGETGEVKCPVASSGFLSEVEAPAAIPPLCGLVAEKSMELGNEAIAEALLKEVALVLTRPNAYSSIGDLMESVNRRIVEAVSKPQTLCSENALGNELAQTLSNVILKHSIDEIHQKNKMIDPNDDRDSSETLESLMESVNQLLFNVIRFTFEKMSHLVQLGGCPPVLSKETIRWRETEPDFQPTDQAICPAWTKAAEYSNCHPLSNLHISSPVINDLVGAGHPKQDSKGPAESGLSQIPTLQSELSCSTSMSAKTPPKEIYLKGSVVEDMKNPPAPPSDNENECRAPPEEEMTPTVGKYSIISQDTEDSIIANAQEKYNGALPLNSELQVCPSLLGDDLLLPAQSTLPEKDPDIYCIADFAEELAETIVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLVTPNISCRSLKKKKESQASGATVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDIPDSVSIFANEVAAKIMNLTEFSLVDGVWQAQGYPRSRLLGGDRWNRLKASSCESIPEEDSNTRAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAVQQACRKNDHLSVKPSCPSKQSSTESITEEFYRYMLRDIEREGKDGPSSRQGSQDWTTGLLSPSLRSPLYYRQSSVPDGRSPGARLTVNVPIKANSLDGFSQHSQQDFLSVQPVGGASSSGLCKSDSCLYRRGGADHITNMLIHETWANSIEALMRKNKIIVDDAGAADAEPPSGGPSLQVEKCAGRLASGRVQSGPALLVQESVDYPRKNSITESSHLPGSSPSKAAPLTSHSSLDFKKETPSCHHAVPLSHTRRPLCSREVPLIQIETDQREECVGEPEHVISEHSPLEEKERCLEEEKVPESGKGEDAALSACRNPSETLDARDVPEAEVSKEGRALDEFSSPPGSSGESMDSWSQLANEEDNPDDTSSFLQLSERSMSELVEEKEILKGQAETMEERASGTPVGTASRQGSLLVINFDLEPECPDTELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVRLVHQKSWKVGDIFHAVVQYCKIHEERRDGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_024981759.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981759.1

ORF Insert Sequence:

ATGTACCAGG TTCCGGAAAC GCAGCAGGGC AGCGCCAGCG CCAGCTCGGG AAGTAGCCTG 
GGCGGCTCCA TCACTGCGTG TAAGAAGGTT CTCCGCAGTA ATAGCCTGCT GGAGTCTACA 
GACTACTGGC TGCAGAATCA GAGAACACCC TGCCAGATTG GTTTCGTGGA AGACAAGTCT 
GAAAACTGCG CTTCTGTCTG CTTTGTGAAC CTGGATGTGA ACAAGGATGC ATGCAGCACG 
GAGCGCCTGC AGCAGAAGTT GGTCAATGTT TCACCAGATC TTCCAAAACT TATAAGCTCC 
ATGAATGTCC AACAGCCAAA AGAAAATGAA ATTGTCCTCC TAAGTGGGTT AACATCTGGA 
AACCTACAGG CAGATTTTGA AGTTTCACAG TGTCCTTGGA TGTCAGATGT CTGCTTGGTC 
CAGTGTGCAA GAGGGAACAG ACCAAACAGT ACCAATTGCA TCATCTTTGA AATCAACAAA 
TTTTTGATTG GTCTGGAACT GGTACAGGAG CGGCAGCTCC ACCTTGAAAC AAACATCTTG 
AAAGTGGAGG ATGACACGAA CTGCTCCTTG TCTTCAATTG AAGAAGACTT TCTCACCGCA 
TCCGAGCACT TGGAGGAGGA AAGTGAAGTG GAAGAGTATA GGAACGGTTA TGAAAATATA 
AATGTCTCAA CCAGTATCTT GGAAAATAAA AAGCCAAAGG AAGCCACTAA GGAGGAATGG 
AATTACAGCA AGGAAAAATT ACTTTATACA TTGGAAGACA AGTATGTTAG CAAATATCAT 
CCACCACTGG TTAAAACAGA AGGATCTCTG GGAAGAGTGG CAGAATATAC AAGTCTGCAG 
AGTCTAGATC CTTCAGCCAA GATATCAAAG TGGAAAGGTG AAGCTGTGGG GAATGAGCGG 
TCAACCACAA AGGATTATTC TTCAGAAAAG TGTAAAAGTC AAGAGGAAAA CTCACAGGCG 
CTATGTAGTC CAGGCAATGC TTATTTCTCA AAGATGGTTA AGAGGGATGG ACTTTCTGCA 
TGTGGGACTT TCTCTGAGCG TGACAGCAAT TTAGACCCAG GAGACCGTGA AGACCGCACA 
AGCTCTCTTC TTCCCTTACA AGATGGAGAA GCCACAACAG GCGAGTATGC CACGAATTTA 
GCGGAATCTG TGATGCAAGA TGCATTTCTT CGACTCTGTA AGTCTCAGCC TGCCCTCCCC 
CAGGAATCTG CAGTCAGTGT TTCTGTAGGA AGTGCTCTGC TTCCCAGTGG CTCTTCCACA 
AAAGATATCG TGGTACCTCA GTCATGGAAC GAGCCCCCCA AAATCGTCAT TGTCCAGAGT 
CCAGAAGGCA GTGATGCTGG CCCTGAGCCA GGCATCTCCT CGTGGCCCGA GACAGAAGTC 
TCCAGTGAAA CTTCAGGTGT CCCCTCTGGA GAGAACTCCA GAAGACGCCC CCAGAGTCCT 
CTAGAAGTAG CATTAGCTTG TGCAGCCACT GTTATTGGGA CCATTTCCAG TCCACAAGCC 
ACAGAAAGAT TCAAAATGGA GCAAGAACCT CTGGTTTCAC ACCATCCACT GGGAGGTGAT 
GAGCCAGGAC AAACTCAGGC CTCTCAAGTA CTCAAGGATC CCTCCCTCAG TGAATACTCG 
TTTCCGTCTG CTTTATGTGG CATGACTCAG GTGGCCAGTG CTGTTGCTGT CTGTGGCCTG 
GGAGAAACAG GAGAGGTGAA GTGCCCGGTG GCTTCCAGTG GCTTCCTGTC TGAAGTTGAG 
GCCCCTGCAG CCATCCCCCC ACTTTGTGGT TTAGTGGCAG AAAAGAGCAT GGAGCTGGGG 
AATGAAGCCA TTGCAGAGGC CTTGCTCAAG GAGGTTGCCT TGGTCTTAAC AAGACCTAAC 
GCCTACAGCA GCATTGGAGA CCTCATGGAG TCAGTGAATA GGAGGATCGT AGAAGCTGTT 
TCAAAGCCTC AGACCTTGTG CTCAGAAAAC GCCCTTGGGA ATGAACTGGC ACAGACCCTG 
TCTAATGTTA TCCTGAAGCA TTCCATTGAT GAAATTCACC AGAAAAATAA AATGATAGAC 
CCCAATGATG ACAGGGACTC ATCTGAAACC CTGGAATCCT TAATGGAAAG TGTAAATCAG 
CTGCTCTTCA ATGTGATACG CTTCACGTTC GAGAAGATGA GCCACCTTGT ACAGCTTGGG 
GGATGTCCCC CTGTCCTTTC TAAGGAGACC ATCAGATGGA GGGAAACCGA ACCAGACTTC 
CAGCCAACTG ATCAAGCCAT TTGTCCAGCA TGGACAAAAG CCGCTGAATA CTCCAATTGC 
CATCCACTTA GCAACCTGCA TATCAGCAGT CCTGTTATCA ATGATCTTGT AGGTGCTGGA 
CATCCGAAGC AAGATAGCAA GGGTCCAGCA GAGTCAGGTC TTTCCCAGAT CCCCACACTG 
CAATCTGAAT TATCCTGCAG TACCAGCATG TCTGCTAAAA CACCACCCAA GGAAATATAT 
CTGAAAGGAT CGGTGGTGGA GGATATGAAA AACCCTCCTG CACCACCCAG TGATAATGAG 
AATGAATGCA GAGCCCCTCC AGAGGAAGAA ATGACACCAA CAGTTGGCAA GTACAGCATC 
ATCTCTCAGG ACACTGAGGA CAGCATCATT GCAAATGCTC AAGAAAAGTA CAATGGTGCC 
TTGCCTCTGA ACAGTGAACT TCAAGTTTGC CCGTCTTTGT TGGGGGATGA TTTGCTGCTT 
CCTGCTCAAT CCACGCTACC AGAAAAAGAC CCAGACATAT ACTGCATTGC AGACTTCGCA 
GAAGAATTAG CAGAGACGAT CGTCTCCATG GCAACGGAAA TCGCAGCGAT TTGCCTTGAC 
AACTCCAATG GAAAACAACC CTGGTTCTGT GCATGGAAAA GAGGGAATGA GTTTCTGGTT 
ACCCCCAACA TCTCCTGCCG ATCCTTGAAG AAGAAGAAAG AGAGCCAGGC CAGCGGGGCC 
ACTGTGAGGA AACACAAGCC CCCGCGACTC AGCGAGATCA AGAGGAAAAC CGATGAGCAC 
CCGGAGCTGA AAGAGAAGCT GATGAACAGG GTGGTGGACG AGTCCATGAA CCTCGAGGAC 
ATCCCAGACT CTGTCAGTAT TTTTGCAAAC GAAGTGGCAG CCAAGATCAT GAACCTCACA 
GAGTTCTCCT TGGTGGACGG TGTGTGGCAA GCACAGGGCT ACCCCCGGAG TCGGTTACTG 
GGTGGGGACA GGTGGAACCG CCTGAAGGCC TCCAGCTGCG AGAGCATTCC AGAGGAGGAC 
TCTAACACCC GGGCCTATGT GAACAGCCTG GGTTTAATGA GTACCCTGAG TCAGCCAGTT 
AGCCGGGCCA GCTCCGTCTC TAAGCAGTCG AGCTGTGAGA GCATCACCGA TGAGTTTTCC 
AGGTTCATGG TGAACCAGAT GGAAAATGAA GGAAGAGGCT TCGAGTTACT GCTGGATTAC 
TACGCAGGCA AGAATGCCAG CAGCATCTTG AACTCAGCTG TGCAACAGGC CTGCCGGAAA 
AACGACCATC TGAGTGTGAA GCCAAGCTGT CCCTCGAAGC AGTCCAGCAC CGAAAGCATC 
ACCGAGGAGT TCTACAGGTA CATGCTGAGG GACATTGAAC GAGAGGGCAA AGACGGCCCC 
TCCTCCAGGC AAGGCAGCCA GGACTGGACC ACTGGCTTGC TCTCGCCTTC TCTGCGGTCC 
CCCTTGTACT ACAGACAGTC GTCTGTGCCC GACGGCAGAT CTCCGGGCGC CAGGCTCACG 
GTGAACGTGC CCATCAAAGC TAACTCCTTA GACGGCTTTT CTCAACACAG CCAGCAAGAC 
TTCCTCAGCG TCCAGCCAGT CGGCGGCGCT TCCTCCTCGG GTCTCTGCAA ATCTGACTCT 
TGCCTGTATC GCAGAGGCGG GGCTGACCAC ATCACAAACA TGTTAATTCA CGAGACGTGG 
GCAAACTCAA TCGAGGCTCT CATGCGGAAG AACAAAATTA TAGTGGATGA TGCCGGCGCG 
GCTGATGCTG AACCTCCTTC AGGGGGCCCC TCCTTGCAAG TGGAAAAGTG TGCGGGCAGA 
TTAGCTTCGG GCAGGGTGCA AAGTGGACCA GCTCTGCTTG TGCAGGAGTC TGTGGATTAT 
CCGAGGAAAA ACTCAATCAC AGAAAGCAGC CATCTCCCAG GGTCGTCCCC AAGCAAAGCT 
GCTCCTCTTA CAAGTCACAG CAGTTTAGAT TTTAAAAAGG AAACTCCCTC ATGCCACCAT 
GCGGTCCCTC TAAGCCACAC CCGGCGACCA CTTTGCTCAA GGGAAGTGCC TTTAATCCAG 
ATCGAAACAG ATCAGAGAGA AGAATGTGTG GGAGAACCTG AACACGTTAT TTCCGAACAC 
AGCCCCCTAG AGGAAAAAGA AAGGTGTTTG GAAGAAGAAA AGGTCCCAGA GTCAGGGAAG 
GGTGAAGACG CTGCCCTGAG TGCCTGCCGG AACCCCAGTG AAACCCTTGA TGCCAGAGAT 
GTACCGGAGG CTGAAGTCTC AAAAGAAGGC AGAGCCCTGG ATGAGTTCTC CAGCCCTCCT 
GGCAGCAGTG GGGAGAGCAT GGACAGTTGG TCCCAGCTCG CCAACGAGGA GGACAACCCA 
GACGACACAA GCAGCTTCCT GCAGCTCAGT GAGCGATCCA TGAGTGAATT GGTAGAAGAA 
AAGGAGATTC TTAAAGGACA GGCAGAAACC ATGGAGGAAC GTGCCTCTGG AACGCCGGTT 
GGAACAGCCA GCCGCCAGGG GAGCCTGCTG GTGATCAACT TTGACCTGGA GCCAGAGTGT 
CCTGACACGG AGCTGCGAGC CACTCTGCAG TGGATAGCTG CATCTGAACT CGGTATCCCC 
ACCATCTACT TTAAGAAATC TCAAGAAAAC AGAATCGAAA AGTTTCTAGA TGTCGTGCGG 
CTGGTCCATC AGAAGTCCTG GAAGGTGGGG GATATCTTCC ATGCAGTGGT TCAGTACTGC 
AAGATACATG AGGAACGGAG GGATGGGACC CCGAGTCTCT TTGATTGGCT CTTGGAACTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_002685643.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002685689.2 Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa02995
Clone ID Related Accession (Same CDS sequence)	XM_002685643.4 , XM_612993.7
Accession Version	XM_002685643.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002685643.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGCA GCTCCCTGCT CTCCGCACCA AGCAGCTTGG AGTCACCCCT GATGTACCAG 
GTTCCGGAAA CGCAGCAGGG CAGCGCCAGC GCCAGCTCGG GAAGTAGCCT GGGCGGCTCC 
ATCACTGCGT GTAAGAAGGT TCTCCGCAGT AATAGCCTGC TGGAGTCTAC AGACTACTGG 
CTGCAGAATC AGAGAACACC CTGCCAGATT GGTTTCGTGG AAGACAAGTC TGAAAACTGC 
GCTTCTGTCT GCTTTGTGAA CCTGGATGTG AACAAGGATG CATGCAGCAC GGAGCGCCTG 
CAGCAGAAGT TGGTCAATGT TTCACCAGAT CTTCCAAAAC TTATAAGCTC CATGAATGTC 
CAACAGCCAA AAGAAAATGA AATTGTCCTC CTAAGTGGGT TAACATCTGG AAACCTACAG 
GCAGATTTTG AAGTTTCACA GTGTCCTTGG ATGTCAGATG TCTGCTTGGT CCAGTGTGCA 
AGAGGGAACA GACCAAACAG TACCAATTGC ATCATCTTTG AAATCAACAA ATTTTTGATT 
GGTCTGGAAC TGGTACAGGA GCGGCAGCTC CACCTTGAAA CAAACATCTT GAAAGTGGAG 
GATGACACGA ACTGCTCCTT GTCTTCAATT GAAGAAGACT TTCTCACCGC ATCCGAGCAC 
TTGGAGGAGG AAAGTGAAGT GGAAGAGTAT AGGAACGGTT ATGAAAATAT AAATGTCTCA 
ACCAGTATCT TGGAAAATAA AAAGCCAAAG GAAGCCACTA AGGAGGAATG GAATTACAGC 
AAGGAAAAAT TACTTTATAC ATTGGAAGAC AAGTATGTTA GCAAATATCA TCCACCACTG 
GTTAAAACAG AAGGATCTCT GGGAAGAGTG GCAGAATATA CAAGTCTGCA GAGTCTAGAT 
CCTTCAGCCA AGATATCAAA GTGGAAAGGT GAAGCTGTGG GGAATGAGCG GTCAACCACA 
AAGGATTATT CTTCAGAAAA GTGTAAAAGT CAAGAGGAAA ACTCACAGGC GCTATGTAGT 
CCAGGCAATG CTTATTTCTC AAAGATGGTT AAGAGGGATG GACTTTCTGC ATGTGGGACT 
TTCTCTGAGC GTGACAGCAA TTTAGACCCA GGAGACCGTG AAGACCGCAC AAGCTCTCTT 
CTTCCCTTAC AAGATGGAGA AGCCACAACA GGCGAGTATG CCACGAATTT AGCGGAATCT 
GTGATGCAAG ATGCATTTCT TCGACTCTGT AAGTCTCAGC CTGCCCTCCC CCAGGAATCT 
GCAGTCAGTG TTTCTGTAGG AAGTGCTCTG CTTCCCAGTG GCTCTTCCAC AAAAGATATC 
GTGGTACCTC AGTCATGGAA CGAGCCCCCC AAAATCGTCA TTGTCCAGAG TCCAGAAGGC 
AGTGATGCTG GCCCTGAGCC AGGCATCTCC TCGTGGCCCG AGACAGAAGT CTCCAGTGAA 
ACTTCAGGTG TCCCCTCTGG AGAGAACTCC AGAAGACGCC CCCAGAGTCC TCTAGAAGTA 
GCATTAGCTT GTGCAGCCAC TGTTATTGGG ACCATTTCCA GTCCACAAGC CACAGAAAGA 
TTCAAAATGG AGCAAGAACC TCTGGTTTCA CACCATCCAC TGGGAGGTGA TGAGCCAGGA 
CAAACTCAGG CCTCTCAAGT ACTCAAGGAT CCCTCCCTCA GTGAATACTC GTTTCCGTCT 
GCTTTATGTG GCATGACTCA GGTGGCCAGT GCTGTTGCTG TCTGTGGCCT GGGAGAAACA 
GGAGAGGTGA AGTGCCCGGT GGCTTCCAGT GGCTTCCTGT CTGAAGTTGA GGCCCCTGCA 
GCCATCCCCC CACTTTGTGG TTTAGTGGCA GAAAAGAGCA TGGAGCTGGG GAATGAAGCC 
ATTGCAGAGG CCTTGCTCAA GGAGGTTGCC TTGGTCTTAA CAAGACCTAA CGCCTACAGC 
AGCATTGGAG ACCTCATGGA GTCAGTGAAT AGGAGGATCG TAGAAGCTGT TTCAAAGCCT 
CAGACCTTGT GCTCAGAAAA CGCCCTTGGG AATGAACTGG CACAGACCCT GTCTAATGTT 
ATCCTGAAGC ATTCCATTGA TGAAATTCAC CAGAAAAATA AAATGATAGA CCCCAATGAT 
GACAGGGACT CATCTGAAAC CCTGGAATCC TTAATGGAAA GTGTAAATCA GCTGCTCTTC 
AATGTGATAC GCTTCACGTT CGAGAAGATG AGCCACCTTG TACAGCTTGG GGGATGTCCC 
CCTGTCCTTT CTAAGGAGAC CATCAGATGG AGGGAAACCG AACCAGACTT CCAGCCAACT 
GATCAAGCCA TTTGTCCAGC ATGGACAAAA GCCGCTGAAT ACTCCAATTG CCATCCACTT 
AGCAACCTGC ATATCAGCAG TCCTGTTATC AATGATCTTG TAGGTGCTGG ACATCCGAAG 
CAAGATAGCA AGGGTCCAGC AGAGTCAGGT CTTTCCCAGA TCCCCACACT GCAATCTGAA 
TTATCCTGCA GTACCAGCAT GTCTGCTAAA ACACCACCCA AGGAAATATA TCTGAAAGGA 
TCGGTGGTGG AGGATATGAA AAACCCTCCT GCACCACCCA GTGATAATGA GAATGAATGC 
AGAGCCCCTC CAGAGGAAGA AATGACACCA ACAGTTGGCA AGTACAGCAT CATCTCTCAG 
GACACTGAGG ACAGCATCAT TGCAAATGCT CAAGAAAAGT ACAATGGTGC CTTGCCTCTG 
AACAGTGAAC TTCAAGTTTG CCCGTCTTTG TTGGGGGATG ATTTGCTGCT TCCTGCTCAA 
TCCACGCTAC CAGAAAAAGA CCCAGACATA TACTGCATTG CAGACTTCGC AGAAGAATTA 
GCAGAGACGA TCGTCTCCAT GGCAACGGAA ATCGCAGCGA TTTGCCTTGA CAACTCCAAT 
GGAAAACAAC CCTGGTTCTG TGCATGGAAA AGAGGGAATG AGTTTCTGGT TACCCCCAAC 
ATCTCCTGCC GATCCTTGAA GAAGAAGAAA GAGAGCCAGG CCAGCGGGGC CACTGTGAGG 
AAACACAAGC CCCCGCGACT CAGCGAGATC AAGAGGAAAA CCGATGAGCA CCCGGAGCTG 
AAAGAGAAGC TGATGAACAG GGTGGTGGAC GAGTCCATGA ACCTCGAGGA CATCCCAGAC 
TCTGTCAGTA TTTTTGCAAA CGAAGTGGCA GCCAAGATCA TGAACCTCAC AGAGTTCTCC 
TTGGTGGACG GTGTGTGGCA AGCACAGGGC TACCCCCGGA GTCGGTTACT GGGTGGGGAC 
AGGTGGAACC GCCTGAAGGC CTCCAGCTGC GAGAGCATTC CAGAGGAGGA CTCTAACACC 
CGGGCCTATG TGAACAGCCT GGGTTTAATG AGTACCCTGA GTCAGCCAGT TAGCCGGGCC 
AGCTCCGTCT CTAAGCAGTC GAGCTGTGAG AGCATCACCG ATGAGTTTTC CAGGTTCATG 
GTGAACCAGA TGGAAAATGA AGGAAGAGGC TTCGAGTTAC TGCTGGATTA CTACGCAGGC 
AAGAATGCCA GCAGCATCTT GAACTCAGCT GTGCAACAGG CCTGCCGGAA AAACGACCAT 
CTGAGTGTGA AGCCAAGCTG TCCCTCGAAG CAGTCCAGCA CCGAAAGCAT CACCGAGGAG 
TTCTACAGGT ACATGCTGAG GGACATTGAA CGAGAGGGCA AAGACGGCCC CTCCTCCAGG 
CAAGGCAGCC AGGACTGGAC CACTGGCTTG CTCTCGCCTT CTCTGCGGTC CCCCTTGTAC 
TACAGACAGT CGTCTGTGCC CGACGGCAGA TCTCCGGGCG CCAGGCTCAC GGTGAACGTG 
CCCATCAAAG CTAACTCCTT AGACGGCTTT TCTCAACACA GCCAGCAAGA CTTCCTCAGC 
GTCCAGCCAG TCGGCGGCGC TTCCTCCTCG GGTCTCTGCA AATCTGACTC TTGCCTGTAT 
CGCAGAGGCG GGGCTGACCA CATCACAAAC ATGTTAATTC ACGAGACGTG GGCAAACTCA 
ATCGAGGCTC TCATGCGGAA GAACAAAATT ATAGTGGATG ATGCCGGCGC GGCTGATGCT 
GAACCTCCTT CAGGGGGCCC CTCCTTGCAA GTGGAAAAGT GTGCGGGCAG ATTAGCTTCG 
GGCAGGGTGC AAAGTGGACC AGCTCTGCTT GTGCAGGAGT CTGTGGATTA TCCGAGGAAA 
AACTCAATCA CAGAAAGCAG CCATCTCCCA GGGTCGTCCC CAAGCAAAGC TGCTCCTCTT 
ACAAGTCACA GCAGTTTAGA TTTTAAAAAG GAAACTCCCT CATGCCACCA TGCGGTCCCT 
CTAAGCCACA CCCGGCGACC ACTTTGCTCA AGGGAAGTGC CTTTAATCCA GATCGAAACA 
GATCAGAGAG AAGAATGTGT GGGAGAACCT GAACACGTTA TTTCCGAACA CAGCCCCCTA 
GAGGAAAAAG AAAGGTGTTT GGAAGAAGAA AAGGTCCCAG AGTCAGGGAA GGGTGAAGAC 
GCTGCCCTGA GTGCCTGCCG GAACCCCAGT GAAACCCTTG ATGCCAGAGA TGTACCGGAG 
GCTGAAGTCT CAAAAGAAGG CAGAGCCCTG GATGAGTTCT CCAGCCCTCC TGGCAGCAGT 
GGGGAGAGCA TGGACAGTTG GTCCCAGCTC GCCAACGAGG AGGACAACCC AGACGACACA 
AGCAGCTTCC TGCAGCTCAG TGAGCGATCC ATGAGCAATG GCAACAGTAG TGCCACTAGC 
AGTCTTGGCA TTATGGACCT GGACATTTAT CAGGAAAGCA TGCCATCTTC TCCCATGATT 
AATGAATTGG TAGAAGAAAA GGAGATTCTT AAAGGACAGG CAGAAACCAT GGAGGAACGT 
GCCTCTGGAA CGCCGGTTGG AACAGCCAGC CGCCAGGGGA GCCTGCTGGT GATCAACTTT 
GACCTGGAGC CAGAGTGTCC TGACACGGAG CTGCGAGCCA CTCTGCAGTG GATAGCTGCA 
TCTGAACTCG GTATCCCCAC CATCTACTTT AAGAAATCTC AAGAAAACAG AATCGAAAAG 
TTTCTAGATG TCGTGCGGCT GGTCCATCAG AAGTCCTGGA AGGTGGGGGA TATCTTCCAT 
GCAGTGGTTC AGTACTGCAA GATACATGAG GAACGGAGGG ATGGGACCCC GAGTCTCTTT 
GATTGGCTCT TGGAACTGGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002685689.2
CDS	581..5704
Translation	MDGSSLLSAPSSLESPLMYQVPETQQGSASASSGSSLGGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDKSENCASVCFVNLDVNKDACSTERLQQKLVNVSPDLPKLISSMNVQQPKENEIVLLSGLTSGNLQADFEVSQCPWMSDVCLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKVEDDTNCSLSSIEEDFLTASEHLEEESEVEEYRNGYENINVSTSILENKKPKEATKEEWNYSKEKLLYTLEDKYVSKYHPPLVKTEGSLGRVAEYTSLQSLDPSAKISKWKGEAVGNERSTTKDYSSEKCKSQEENSQALCSPGNAYFSKMVKRDGLSACGTFSERDSNLDPGDREDRTSSLLPLQDGEATTGEYATNLAESVMQDAFLRLCKSQPALPQESAVSVSVGSALLPSGSSTKDIVVPQSWNEPPKIVIVQSPEGSDAGPEPGISSWPETEVSSETSGVPSGENSRRRPQSPLEVALACAATVIGTISSPQATERFKMEQEPLVSHHPLGGDEPGQTQASQVLKDPSLSEYSFPSALCGMTQVASAVAVCGLGETGEVKCPVASSGFLSEVEAPAAIPPLCGLVAEKSMELGNEAIAEALLKEVALVLTRPNAYSSIGDLMESVNRRIVEAVSKPQTLCSENALGNELAQTLSNVILKHSIDEIHQKNKMIDPNDDRDSSETLESLMESVNQLLFNVIRFTFEKMSHLVQLGGCPPVLSKETIRWRETEPDFQPTDQAICPAWTKAAEYSNCHPLSNLHISSPVINDLVGAGHPKQDSKGPAESGLSQIPTLQSELSCSTSMSAKTPPKEIYLKGSVVEDMKNPPAPPSDNENECRAPPEEEMTPTVGKYSIISQDTEDSIIANAQEKYNGALPLNSELQVCPSLLGDDLLLPAQSTLPEKDPDIYCIADFAEELAETIVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLVTPNISCRSLKKKKESQASGATVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDIPDSVSIFANEVAAKIMNLTEFSLVDGVWQAQGYPRSRLLGGDRWNRLKASSCESIPEEDSNTRAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAVQQACRKNDHLSVKPSCPSKQSSTESITEEFYRYMLRDIEREGKDGPSSRQGSQDWTTGLLSPSLRSPLYYRQSSVPDGRSPGARLTVNVPIKANSLDGFSQHSQQDFLSVQPVGGASSSGLCKSDSCLYRRGGADHITNMLIHETWANSIEALMRKNKIIVDDAGAADAEPPSGGPSLQVEKCAGRLASGRVQSGPALLVQESVDYPRKNSITESSHLPGSSPSKAAPLTSHSSLDFKKETPSCHHAVPLSHTRRPLCSREVPLIQIETDQREECVGEPEHVISEHSPLEEKERCLEEEKVPESGKGEDAALSACRNPSETLDARDVPEAEVSKEGRALDEFSSPPGSSGESMDSWSQLANEEDNPDDTSSFLQLSERSMSNGNSSATSSLGIMDLDIYQESMPSSPMINELVEEKEILKGQAETMEERASGTPVGTASRQGSLLVINFDLEPECPDTELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVRLVHQKSWKVGDIFHAVVQYCKIHEERRDGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_002685643.4
Organism	Bos taurus(cattle)
Definition	Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002685643.4

ORF Insert Sequence:

ATGGATGGCA GCTCCCTGCT CTCCGCACCA AGCAGCTTGG AGTCACCCCT GATGTACCAG 
GTTCCGGAAA CGCAGCAGGG CAGCGCCAGC GCCAGCTCGG GAAGTAGCCT GGGCGGCTCC 
ATCACTGCGT GTAAGAAGGT TCTCCGCAGT AATAGCCTGC TGGAGTCTAC AGACTACTGG 
CTGCAGAATC AGAGAACACC CTGCCAGATT GGTTTCGTGG AAGACAAGTC TGAAAACTGC 
GCTTCTGTCT GCTTTGTGAA CCTGGATGTG AACAAGGATG CATGCAGCAC GGAGCGCCTG 
CAGCAGAAGT TGGTCAATGT TTCACCAGAT CTTCCAAAAC TTATAAGCTC CATGAATGTC 
CAACAGCCAA AAGAAAATGA AATTGTCCTC CTAAGTGGGT TAACATCTGG AAACCTACAG 
GCAGATTTTG AAGTTTCACA GTGTCCTTGG ATGTCAGATG TCTGCTTGGT CCAGTGTGCA 
AGAGGGAACA GACCAAACAG TACCAATTGC ATCATCTTTG AAATCAACAA ATTTTTGATT 
GGTCTGGAAC TGGTACAGGA GCGGCAGCTC CACCTTGAAA CAAACATCTT GAAAGTGGAG 
GATGACACGA ACTGCTCCTT GTCTTCAATT GAAGAAGACT TTCTCACCGC ATCCGAGCAC 
TTGGAGGAGG AAAGTGAAGT GGAAGAGTAT AGGAACGGTT ATGAAAATAT AAATGTCTCA 
ACCAGTATCT TGGAAAATAA AAAGCCAAAG GAAGCCACTA AGGAGGAATG GAATTACAGC 
AAGGAAAAAT TACTTTATAC ATTGGAAGAC AAGTATGTTA GCAAATATCA TCCACCACTG 
GTTAAAACAG AAGGATCTCT GGGAAGAGTG GCAGAATATA CAAGTCTGCA GAGTCTAGAT 
CCTTCAGCCA AGATATCAAA GTGGAAAGGT GAAGCTGTGG GGAATGAGCG GTCAACCACA 
AAGGATTATT CTTCAGAAAA GTGTAAAAGT CAAGAGGAAA ACTCACAGGC GCTATGTAGT 
CCAGGCAATG CTTATTTCTC AAAGATGGTT AAGAGGGATG GACTTTCTGC ATGTGGGACT 
TTCTCTGAGC GTGACAGCAA TTTAGACCCA GGAGACCGTG AAGACCGCAC AAGCTCTCTT 
CTTCCCTTAC AAGATGGAGA AGCCACAACA GGCGAGTATG CCACGAATTT AGCGGAATCT 
GTGATGCAAG ATGCATTTCT TCGACTCTGT AAGTCTCAGC CTGCCCTCCC CCAGGAATCT 
GCAGTCAGTG TTTCTGTAGG AAGTGCTCTG CTTCCCAGTG GCTCTTCCAC AAAAGATATC 
GTGGTACCTC AGTCATGGAA CGAGCCCCCC AAAATCGTCA TTGTCCAGAG TCCAGAAGGC 
AGTGATGCTG GCCCTGAGCC AGGCATCTCC TCGTGGCCCG AGACAGAAGT CTCCAGTGAA 
ACTTCAGGTG TCCCCTCTGG AGAGAACTCC AGAAGACGCC CCCAGAGTCC TCTAGAAGTA 
GCATTAGCTT GTGCAGCCAC TGTTATTGGG ACCATTTCCA GTCCACAAGC CACAGAAAGA 
TTCAAAATGG AGCAAGAACC TCTGGTTTCA CACCATCCAC TGGGAGGTGA TGAGCCAGGA 
CAAACTCAGG CCTCTCAAGT ACTCAAGGAT CCCTCCCTCA GTGAATACTC GTTTCCGTCT 
GCTTTATGTG GCATGACTCA GGTGGCCAGT GCTGTTGCTG TCTGTGGCCT GGGAGAAACA 
GGAGAGGTGA AGTGCCCGGT GGCTTCCAGT GGCTTCCTGT CTGAAGTTGA GGCCCCTGCA 
GCCATCCCCC CACTTTGTGG TTTAGTGGCA GAAAAGAGCA TGGAGCTGGG GAATGAAGCC 
ATTGCAGAGG CCTTGCTCAA GGAGGTTGCC TTGGTCTTAA CAAGACCTAA CGCCTACAGC 
AGCATTGGAG ACCTCATGGA GTCAGTGAAT AGGAGGATCG TAGAAGCTGT TTCAAAGCCT 
CAGACCTTGT GCTCAGAAAA CGCCCTTGGG AATGAACTGG CACAGACCCT GTCTAATGTT 
ATCCTGAAGC ATTCCATTGA TGAAATTCAC CAGAAAAATA AAATGATAGA CCCCAATGAT 
GACAGGGACT CATCTGAAAC CCTGGAATCC TTAATGGAAA GTGTAAATCA GCTGCTCTTC 
AATGTGATAC GCTTCACGTT CGAGAAGATG AGCCACCTTG TACAGCTTGG GGGATGTCCC 
CCTGTCCTTT CTAAGGAGAC CATCAGATGG AGGGAAACCG AACCAGACTT CCAGCCAACT 
GATCAAGCCA TTTGTCCAGC ATGGACAAAA GCCGCTGAAT ACTCCAATTG CCATCCACTT 
AGCAACCTGC ATATCAGCAG TCCTGTTATC AATGATCTTG TAGGTGCTGG ACATCCGAAG 
CAAGATAGCA AGGGTCCAGC AGAGTCAGGT CTTTCCCAGA TCCCCACACT GCAATCTGAA 
TTATCCTGCA GTACCAGCAT GTCTGCTAAA ACACCACCCA AGGAAATATA TCTGAAAGGA 
TCGGTGGTGG AGGATATGAA AAACCCTCCT GCACCACCCA GTGATAATGA GAATGAATGC 
AGAGCCCCTC CAGAGGAAGA AATGACACCA ACAGTTGGCA AGTACAGCAT CATCTCTCAG 
GACACTGAGG ACAGCATCAT TGCAAATGCT CAAGAAAAGT ACAATGGTGC CTTGCCTCTG 
AACAGTGAAC TTCAAGTTTG CCCGTCTTTG TTGGGGGATG ATTTGCTGCT TCCTGCTCAA 
TCCACGCTAC CAGAAAAAGA CCCAGACATA TACTGCATTG CAGACTTCGC AGAAGAATTA 
GCAGAGACGA TCGTCTCCAT GGCAACGGAA ATCGCAGCGA TTTGCCTTGA CAACTCCAAT 
GGAAAACAAC CCTGGTTCTG TGCATGGAAA AGAGGGAATG AGTTTCTGGT TACCCCCAAC 
ATCTCCTGCC GATCCTTGAA GAAGAAGAAA GAGAGCCAGG CCAGCGGGGC CACTGTGAGG 
AAACACAAGC CCCCGCGACT CAGCGAGATC AAGAGGAAAA CCGATGAGCA CCCGGAGCTG 
AAAGAGAAGC TGATGAACAG GGTGGTGGAC GAGTCCATGA ACCTCGAGGA CATCCCAGAC 
TCTGTCAGTA TTTTTGCAAA CGAAGTGGCA GCCAAGATCA TGAACCTCAC AGAGTTCTCC 
TTGGTGGACG GTGTGTGGCA AGCACAGGGC TACCCCCGGA GTCGGTTACT GGGTGGGGAC 
AGGTGGAACC GCCTGAAGGC CTCCAGCTGC GAGAGCATTC CAGAGGAGGA CTCTAACACC 
CGGGCCTATG TGAACAGCCT GGGTTTAATG AGTACCCTGA GTCAGCCAGT TAGCCGGGCC 
AGCTCCGTCT CTAAGCAGTC GAGCTGTGAG AGCATCACCG ATGAGTTTTC CAGGTTCATG 
GTGAACCAGA TGGAAAATGA AGGAAGAGGC TTCGAGTTAC TGCTGGATTA CTACGCAGGC 
AAGAATGCCA GCAGCATCTT GAACTCAGCT GTGCAACAGG CCTGCCGGAA AAACGACCAT 
CTGAGTGTGA AGCCAAGCTG TCCCTCGAAG CAGTCCAGCA CCGAAAGCAT CACCGAGGAG 
TTCTACAGGT ACATGCTGAG GGACATTGAA CGAGAGGGCA AAGACGGCCC CTCCTCCAGG 
CAAGGCAGCC AGGACTGGAC CACTGGCTTG CTCTCGCCTT CTCTGCGGTC CCCCTTGTAC 
TACAGACAGT CGTCTGTGCC CGACGGCAGA TCTCCGGGCG CCAGGCTCAC GGTGAACGTG 
CCCATCAAAG CTAACTCCTT AGACGGCTTT TCTCAACACA GCCAGCAAGA CTTCCTCAGC 
GTCCAGCCAG TCGGCGGCGC TTCCTCCTCG GGTCTCTGCA AATCTGACTC TTGCCTGTAT 
CGCAGAGGCG GGGCTGACCA CATCACAAAC ATGTTAATTC ACGAGACGTG GGCAAACTCA 
ATCGAGGCTC TCATGCGGAA GAACAAAATT ATAGTGGATG ATGCCGGCGC GGCTGATGCT 
GAACCTCCTT CAGGGGGCCC CTCCTTGCAA GTGGAAAAGT GTGCGGGCAG ATTAGCTTCG 
GGCAGGGTGC AAAGTGGACC AGCTCTGCTT GTGCAGGAGT CTGTGGATTA TCCGAGGAAA 
AACTCAATCA CAGAAAGCAG CCATCTCCCA GGGTCGTCCC CAAGCAAAGC TGCTCCTCTT 
ACAAGTCACA GCAGTTTAGA TTTTAAAAAG GAAACTCCCT CATGCCACCA TGCGGTCCCT 
CTAAGCCACA CCCGGCGACC ACTTTGCTCA AGGGAAGTGC CTTTAATCCA GATCGAAACA 
GATCAGAGAG AAGAATGTGT GGGAGAACCT GAACACGTTA TTTCCGAACA CAGCCCCCTA 
GAGGAAAAAG AAAGGTGTTT GGAAGAAGAA AAGGTCCCAG AGTCAGGGAA GGGTGAAGAC 
GCTGCCCTGA GTGCCTGCCG GAACCCCAGT GAAACCCTTG ATGCCAGAGA TGTACCGGAG 
GCTGAAGTCT CAAAAGAAGG CAGAGCCCTG GATGAGTTCT CCAGCCCTCC TGGCAGCAGT 
GGGGAGAGCA TGGACAGTTG GTCCCAGCTC GCCAACGAGG AGGACAACCC AGACGACACA 
AGCAGCTTCC TGCAGCTCAG TGAGCGATCC ATGAGCAATG GCAACAGTAG TGCCACTAGC 
AGTCTTGGCA TTATGGACCT GGACATTTAT CAGGAAAGCA TGCCATCTTC TCCCATGATT 
AATGAATTGG TAGAAGAAAA GGAGATTCTT AAAGGACAGG CAGAAACCAT GGAGGAACGT 
GCCTCTGGAA CGCCGGTTGG AACAGCCAGC CGCCAGGGGA GCCTGCTGGT GATCAACTTT 
GACCTGGAGC CAGAGTGTCC TGACACGGAG CTGCGAGCCA CTCTGCAGTG GATAGCTGCA 
TCTGAACTCG GTATCCCCAC CATCTACTTT AAGAAATCTC AAGAAAACAG AATCGAAAAG 
TTTCTAGATG TCGTGCGGCT GGTCCATCAG AAGTCCTGGA AGGTGGGGGA TATCTTCCAT 
GCAGTGGTTC AGTACTGCAA GATACATGAG GAACGGAGGG ATGGGACCCC GAGTCTCTTT 
GATTGGCTCT TGGAACTGGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_612993.7 ) cDNA ORF clone, Bos taurus(cattle) -> XP_612993.5 Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

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CloneID	OBa02995
Clone ID Related Accession (Same CDS sequence)	XM_002685643.4 , XM_612993.7
Accession Version	XM_612993.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_612993.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGCA GCTCCCTGCT CTCCGCACCA AGCAGCTTGG AGTCACCCCT GATGTACCAG 
GTTCCGGAAA CGCAGCAGGG CAGCGCCAGC GCCAGCTCGG GAAGTAGCCT GGGCGGCTCC 
ATCACTGCGT GTAAGAAGGT TCTCCGCAGT AATAGCCTGC TGGAGTCTAC AGACTACTGG 
CTGCAGAATC AGAGAACACC CTGCCAGATT GGTTTCGTGG AAGACAAGTC TGAAAACTGC 
GCTTCTGTCT GCTTTGTGAA CCTGGATGTG AACAAGGATG CATGCAGCAC GGAGCGCCTG 
CAGCAGAAGT TGGTCAATGT TTCACCAGAT CTTCCAAAAC TTATAAGCTC CATGAATGTC 
CAACAGCCAA AAGAAAATGA AATTGTCCTC CTAAGTGGGT TAACATCTGG AAACCTACAG 
GCAGATTTTG AAGTTTCACA GTGTCCTTGG ATGTCAGATG TCTGCTTGGT CCAGTGTGCA 
AGAGGGAACA GACCAAACAG TACCAATTGC ATCATCTTTG AAATCAACAA ATTTTTGATT 
GGTCTGGAAC TGGTACAGGA GCGGCAGCTC CACCTTGAAA CAAACATCTT GAAAGTGGAG 
GATGACACGA ACTGCTCCTT GTCTTCAATT GAAGAAGACT TTCTCACCGC ATCCGAGCAC 
TTGGAGGAGG AAAGTGAAGT GGAAGAGTAT AGGAACGGTT ATGAAAATAT AAATGTCTCA 
ACCAGTATCT TGGAAAATAA AAAGCCAAAG GAAGCCACTA AGGAGGAATG GAATTACAGC 
AAGGAAAAAT TACTTTATAC ATTGGAAGAC AAGTATGTTA GCAAATATCA TCCACCACTG 
GTTAAAACAG AAGGATCTCT GGGAAGAGTG GCAGAATATA CAAGTCTGCA GAGTCTAGAT 
CCTTCAGCCA AGATATCAAA GTGGAAAGGT GAAGCTGTGG GGAATGAGCG GTCAACCACA 
AAGGATTATT CTTCAGAAAA GTGTAAAAGT CAAGAGGAAA ACTCACAGGC GCTATGTAGT 
CCAGGCAATG CTTATTTCTC AAAGATGGTT AAGAGGGATG GACTTTCTGC ATGTGGGACT 
TTCTCTGAGC GTGACAGCAA TTTAGACCCA GGAGACCGTG AAGACCGCAC AAGCTCTCTT 
CTTCCCTTAC AAGATGGAGA AGCCACAACA GGCGAGTATG CCACGAATTT AGCGGAATCT 
GTGATGCAAG ATGCATTTCT TCGACTCTGT AAGTCTCAGC CTGCCCTCCC CCAGGAATCT 
GCAGTCAGTG TTTCTGTAGG AAGTGCTCTG CTTCCCAGTG GCTCTTCCAC AAAAGATATC 
GTGGTACCTC AGTCATGGAA CGAGCCCCCC AAAATCGTCA TTGTCCAGAG TCCAGAAGGC 
AGTGATGCTG GCCCTGAGCC AGGCATCTCC TCGTGGCCCG AGACAGAAGT CTCCAGTGAA 
ACTTCAGGTG TCCCCTCTGG AGAGAACTCC AGAAGACGCC CCCAGAGTCC TCTAGAAGTA 
GCATTAGCTT GTGCAGCCAC TGTTATTGGG ACCATTTCCA GTCCACAAGC CACAGAAAGA 
TTCAAAATGG AGCAAGAACC TCTGGTTTCA CACCATCCAC TGGGAGGTGA TGAGCCAGGA 
CAAACTCAGG CCTCTCAAGT ACTCAAGGAT CCCTCCCTCA GTGAATACTC GTTTCCGTCT 
GCTTTATGTG GCATGACTCA GGTGGCCAGT GCTGTTGCTG TCTGTGGCCT GGGAGAAACA 
GGAGAGGTGA AGTGCCCGGT GGCTTCCAGT GGCTTCCTGT CTGAAGTTGA GGCCCCTGCA 
GCCATCCCCC CACTTTGTGG TTTAGTGGCA GAAAAGAGCA TGGAGCTGGG GAATGAAGCC 
ATTGCAGAGG CCTTGCTCAA GGAGGTTGCC TTGGTCTTAA CAAGACCTAA CGCCTACAGC 
AGCATTGGAG ACCTCATGGA GTCAGTGAAT AGGAGGATCG TAGAAGCTGT TTCAAAGCCT 
CAGACCTTGT GCTCAGAAAA CGCCCTTGGG AATGAACTGG CACAGACCCT GTCTAATGTT 
ATCCTGAAGC ATTCCATTGA TGAAATTCAC CAGAAAAATA AAATGATAGA CCCCAATGAT 
GACAGGGACT CATCTGAAAC CCTGGAATCC TTAATGGAAA GTGTAAATCA GCTGCTCTTC 
AATGTGATAC GCTTCACGTT CGAGAAGATG AGCCACCTTG TACAGCTTGG GGGATGTCCC 
CCTGTCCTTT CTAAGGAGAC CATCAGATGG AGGGAAACCG AACCAGACTT CCAGCCAACT 
GATCAAGCCA TTTGTCCAGC ATGGACAAAA GCCGCTGAAT ACTCCAATTG CCATCCACTT 
AGCAACCTGC ATATCAGCAG TCCTGTTATC AATGATCTTG TAGGTGCTGG ACATCCGAAG 
CAAGATAGCA AGGGTCCAGC AGAGTCAGGT CTTTCCCAGA TCCCCACACT GCAATCTGAA 
TTATCCTGCA GTACCAGCAT GTCTGCTAAA ACACCACCCA AGGAAATATA TCTGAAAGGA 
TCGGTGGTGG AGGATATGAA AAACCCTCCT GCACCACCCA GTGATAATGA GAATGAATGC 
AGAGCCCCTC CAGAGGAAGA AATGACACCA ACAGTTGGCA AGTACAGCAT CATCTCTCAG 
GACACTGAGG ACAGCATCAT TGCAAATGCT CAAGAAAAGT ACAATGGTGC CTTGCCTCTG 
AACAGTGAAC TTCAAGTTTG CCCGTCTTTG TTGGGGGATG ATTTGCTGCT TCCTGCTCAA 
TCCACGCTAC CAGAAAAAGA CCCAGACATA TACTGCATTG CAGACTTCGC AGAAGAATTA 
GCAGAGACGA TCGTCTCCAT GGCAACGGAA ATCGCAGCGA TTTGCCTTGA CAACTCCAAT 
GGAAAACAAC CCTGGTTCTG TGCATGGAAA AGAGGGAATG AGTTTCTGGT TACCCCCAAC 
ATCTCCTGCC GATCCTTGAA GAAGAAGAAA GAGAGCCAGG CCAGCGGGGC CACTGTGAGG 
AAACACAAGC CCCCGCGACT CAGCGAGATC AAGAGGAAAA CCGATGAGCA CCCGGAGCTG 
AAAGAGAAGC TGATGAACAG GGTGGTGGAC GAGTCCATGA ACCTCGAGGA CATCCCAGAC 
TCTGTCAGTA TTTTTGCAAA CGAAGTGGCA GCCAAGATCA TGAACCTCAC AGAGTTCTCC 
TTGGTGGACG GTGTGTGGCA AGCACAGGGC TACCCCCGGA GTCGGTTACT GGGTGGGGAC 
AGGTGGAACC GCCTGAAGGC CTCCAGCTGC GAGAGCATTC CAGAGGAGGA CTCTAACACC 
CGGGCCTATG TGAACAGCCT GGGTTTAATG AGTACCCTGA GTCAGCCAGT TAGCCGGGCC 
AGCTCCGTCT CTAAGCAGTC GAGCTGTGAG AGCATCACCG ATGAGTTTTC CAGGTTCATG 
GTGAACCAGA TGGAAAATGA AGGAAGAGGC TTCGAGTTAC TGCTGGATTA CTACGCAGGC 
AAGAATGCCA GCAGCATCTT GAACTCAGCT GTGCAACAGG CCTGCCGGAA AAACGACCAT 
CTGAGTGTGA AGCCAAGCTG TCCCTCGAAG CAGTCCAGCA CCGAAAGCAT CACCGAGGAG 
TTCTACAGGT ACATGCTGAG GGACATTGAA CGAGAGGGCA AAGACGGCCC CTCCTCCAGG 
CAAGGCAGCC AGGACTGGAC CACTGGCTTG CTCTCGCCTT CTCTGCGGTC CCCCTTGTAC 
TACAGACAGT CGTCTGTGCC CGACGGCAGA TCTCCGGGCG CCAGGCTCAC GGTGAACGTG 
CCCATCAAAG CTAACTCCTT AGACGGCTTT TCTCAACACA GCCAGCAAGA CTTCCTCAGC 
GTCCAGCCAG TCGGCGGCGC TTCCTCCTCG GGTCTCTGCA AATCTGACTC TTGCCTGTAT 
CGCAGAGGCG GGGCTGACCA CATCACAAAC ATGTTAATTC ACGAGACGTG GGCAAACTCA 
ATCGAGGCTC TCATGCGGAA GAACAAAATT ATAGTGGATG ATGCCGGCGC GGCTGATGCT 
GAACCTCCTT CAGGGGGCCC CTCCTTGCAA GTGGAAAAGT GTGCGGGCAG ATTAGCTTCG 
GGCAGGGTGC AAAGTGGACC AGCTCTGCTT GTGCAGGAGT CTGTGGATTA TCCGAGGAAA 
AACTCAATCA CAGAAAGCAG CCATCTCCCA GGGTCGTCCC CAAGCAAAGC TGCTCCTCTT 
ACAAGTCACA GCAGTTTAGA TTTTAAAAAG GAAACTCCCT CATGCCACCA TGCGGTCCCT 
CTAAGCCACA CCCGGCGACC ACTTTGCTCA AGGGAAGTGC CTTTAATCCA GATCGAAACA 
GATCAGAGAG AAGAATGTGT GGGAGAACCT GAACACGTTA TTTCCGAACA CAGCCCCCTA 
GAGGAAAAAG AAAGGTGTTT GGAAGAAGAA AAGGTCCCAG AGTCAGGGAA GGGTGAAGAC 
GCTGCCCTGA GTGCCTGCCG GAACCCCAGT GAAACCCTTG ATGCCAGAGA TGTACCGGAG 
GCTGAAGTCT CAAAAGAAGG CAGAGCCCTG GATGAGTTCT CCAGCCCTCC TGGCAGCAGT 
GGGGAGAGCA TGGACAGTTG GTCCCAGCTC GCCAACGAGG AGGACAACCC AGACGACACA 
AGCAGCTTCC TGCAGCTCAG TGAGCGATCC ATGAGCAATG GCAACAGTAG TGCCACTAGC 
AGTCTTGGCA TTATGGACCT GGACATTTAT CAGGAAAGCA TGCCATCTTC TCCCATGATT 
AATGAATTGG TAGAAGAAAA GGAGATTCTT AAAGGACAGG CAGAAACCAT GGAGGAACGT 
GCCTCTGGAA CGCCGGTTGG AACAGCCAGC CGCCAGGGGA GCCTGCTGGT GATCAACTTT 
GACCTGGAGC CAGAGTGTCC TGACACGGAG CTGCGAGCCA CTCTGCAGTG GATAGCTGCA 
TCTGAACTCG GTATCCCCAC CATCTACTTT AAGAAATCTC AAGAAAACAG AATCGAAAAG 
TTTCTAGATG TCGTGCGGCT GGTCCATCAG AAGTCCTGGA AGGTGGGGGA TATCTTCCAT 
GCAGTGGTTC AGTACTGCAA GATACATGAG GAACGGAGGG ATGGGACCCC GAGTCTCTTT 
GATTGGCTCT TGGAACTGGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_612993.5
CDS	581..5704
Translation	MDGSSLLSAPSSLESPLMYQVPETQQGSASASSGSSLGGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDKSENCASVCFVNLDVNKDACSTERLQQKLVNVSPDLPKLISSMNVQQPKENEIVLLSGLTSGNLQADFEVSQCPWMSDVCLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKVEDDTNCSLSSIEEDFLTASEHLEEESEVEEYRNGYENINVSTSILENKKPKEATKEEWNYSKEKLLYTLEDKYVSKYHPPLVKTEGSLGRVAEYTSLQSLDPSAKISKWKGEAVGNERSTTKDYSSEKCKSQEENSQALCSPGNAYFSKMVKRDGLSACGTFSERDSNLDPGDREDRTSSLLPLQDGEATTGEYATNLAESVMQDAFLRLCKSQPALPQESAVSVSVGSALLPSGSSTKDIVVPQSWNEPPKIVIVQSPEGSDAGPEPGISSWPETEVSSETSGVPSGENSRRRPQSPLEVALACAATVIGTISSPQATERFKMEQEPLVSHHPLGGDEPGQTQASQVLKDPSLSEYSFPSALCGMTQVASAVAVCGLGETGEVKCPVASSGFLSEVEAPAAIPPLCGLVAEKSMELGNEAIAEALLKEVALVLTRPNAYSSIGDLMESVNRRIVEAVSKPQTLCSENALGNELAQTLSNVILKHSIDEIHQKNKMIDPNDDRDSSETLESLMESVNQLLFNVIRFTFEKMSHLVQLGGCPPVLSKETIRWRETEPDFQPTDQAICPAWTKAAEYSNCHPLSNLHISSPVINDLVGAGHPKQDSKGPAESGLSQIPTLQSELSCSTSMSAKTPPKEIYLKGSVVEDMKNPPAPPSDNENECRAPPEEEMTPTVGKYSIISQDTEDSIIANAQEKYNGALPLNSELQVCPSLLGDDLLLPAQSTLPEKDPDIYCIADFAEELAETIVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLVTPNISCRSLKKKKESQASGATVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDIPDSVSIFANEVAAKIMNLTEFSLVDGVWQAQGYPRSRLLGGDRWNRLKASSCESIPEEDSNTRAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAVQQACRKNDHLSVKPSCPSKQSSTESITEEFYRYMLRDIEREGKDGPSSRQGSQDWTTGLLSPSLRSPLYYRQSSVPDGRSPGARLTVNVPIKANSLDGFSQHSQQDFLSVQPVGGASSSGLCKSDSCLYRRGGADHITNMLIHETWANSIEALMRKNKIIVDDAGAADAEPPSGGPSLQVEKCAGRLASGRVQSGPALLVQESVDYPRKNSITESSHLPGSSPSKAAPLTSHSSLDFKKETPSCHHAVPLSHTRRPLCSREVPLIQIETDQREECVGEPEHVISEHSPLEEKERCLEEEKVPESGKGEDAALSACRNPSETLDARDVPEAEVSKEGRALDEFSSPPGSSGESMDSWSQLANEEDNPDDTSSFLQLSERSMSNGNSSATSSLGIMDLDIYQESMPSSPMINELVEEKEILKGQAETMEERASGTPVGTASRQGSLLVINFDLEPECPDTELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVRLVHQKSWKVGDIFHAVVQYCKIHEERRDGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_612993.7
Organism	Bos taurus(cattle)
Definition	Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_612993.7

ORF Insert Sequence:

ATGGATGGCA GCTCCCTGCT CTCCGCACCA AGCAGCTTGG AGTCACCCCT GATGTACCAG 
GTTCCGGAAA CGCAGCAGGG CAGCGCCAGC GCCAGCTCGG GAAGTAGCCT GGGCGGCTCC 
ATCACTGCGT GTAAGAAGGT TCTCCGCAGT AATAGCCTGC TGGAGTCTAC AGACTACTGG 
CTGCAGAATC AGAGAACACC CTGCCAGATT GGTTTCGTGG AAGACAAGTC TGAAAACTGC 
GCTTCTGTCT GCTTTGTGAA CCTGGATGTG AACAAGGATG CATGCAGCAC GGAGCGCCTG 
CAGCAGAAGT TGGTCAATGT TTCACCAGAT CTTCCAAAAC TTATAAGCTC CATGAATGTC 
CAACAGCCAA AAGAAAATGA AATTGTCCTC CTAAGTGGGT TAACATCTGG AAACCTACAG 
GCAGATTTTG AAGTTTCACA GTGTCCTTGG ATGTCAGATG TCTGCTTGGT CCAGTGTGCA 
AGAGGGAACA GACCAAACAG TACCAATTGC ATCATCTTTG AAATCAACAA ATTTTTGATT 
GGTCTGGAAC TGGTACAGGA GCGGCAGCTC CACCTTGAAA CAAACATCTT GAAAGTGGAG 
GATGACACGA ACTGCTCCTT GTCTTCAATT GAAGAAGACT TTCTCACCGC ATCCGAGCAC 
TTGGAGGAGG AAAGTGAAGT GGAAGAGTAT AGGAACGGTT ATGAAAATAT AAATGTCTCA 
ACCAGTATCT TGGAAAATAA AAAGCCAAAG GAAGCCACTA AGGAGGAATG GAATTACAGC 
AAGGAAAAAT TACTTTATAC ATTGGAAGAC AAGTATGTTA GCAAATATCA TCCACCACTG 
GTTAAAACAG AAGGATCTCT GGGAAGAGTG GCAGAATATA CAAGTCTGCA GAGTCTAGAT 
CCTTCAGCCA AGATATCAAA GTGGAAAGGT GAAGCTGTGG GGAATGAGCG GTCAACCACA 
AAGGATTATT CTTCAGAAAA GTGTAAAAGT CAAGAGGAAA ACTCACAGGC GCTATGTAGT 
CCAGGCAATG CTTATTTCTC AAAGATGGTT AAGAGGGATG GACTTTCTGC ATGTGGGACT 
TTCTCTGAGC GTGACAGCAA TTTAGACCCA GGAGACCGTG AAGACCGCAC AAGCTCTCTT 
CTTCCCTTAC AAGATGGAGA AGCCACAACA GGCGAGTATG CCACGAATTT AGCGGAATCT 
GTGATGCAAG ATGCATTTCT TCGACTCTGT AAGTCTCAGC CTGCCCTCCC CCAGGAATCT 
GCAGTCAGTG TTTCTGTAGG AAGTGCTCTG CTTCCCAGTG GCTCTTCCAC AAAAGATATC 
GTGGTACCTC AGTCATGGAA CGAGCCCCCC AAAATCGTCA TTGTCCAGAG TCCAGAAGGC 
AGTGATGCTG GCCCTGAGCC AGGCATCTCC TCGTGGCCCG AGACAGAAGT CTCCAGTGAA 
ACTTCAGGTG TCCCCTCTGG AGAGAACTCC AGAAGACGCC CCCAGAGTCC TCTAGAAGTA 
GCATTAGCTT GTGCAGCCAC TGTTATTGGG ACCATTTCCA GTCCACAAGC CACAGAAAGA 
TTCAAAATGG AGCAAGAACC TCTGGTTTCA CACCATCCAC TGGGAGGTGA TGAGCCAGGA 
CAAACTCAGG CCTCTCAAGT ACTCAAGGAT CCCTCCCTCA GTGAATACTC GTTTCCGTCT 
GCTTTATGTG GCATGACTCA GGTGGCCAGT GCTGTTGCTG TCTGTGGCCT GGGAGAAACA 
GGAGAGGTGA AGTGCCCGGT GGCTTCCAGT GGCTTCCTGT CTGAAGTTGA GGCCCCTGCA 
GCCATCCCCC CACTTTGTGG TTTAGTGGCA GAAAAGAGCA TGGAGCTGGG GAATGAAGCC 
ATTGCAGAGG CCTTGCTCAA GGAGGTTGCC TTGGTCTTAA CAAGACCTAA CGCCTACAGC 
AGCATTGGAG ACCTCATGGA GTCAGTGAAT AGGAGGATCG TAGAAGCTGT TTCAAAGCCT 
CAGACCTTGT GCTCAGAAAA CGCCCTTGGG AATGAACTGG CACAGACCCT GTCTAATGTT 
ATCCTGAAGC ATTCCATTGA TGAAATTCAC CAGAAAAATA AAATGATAGA CCCCAATGAT 
GACAGGGACT CATCTGAAAC CCTGGAATCC TTAATGGAAA GTGTAAATCA GCTGCTCTTC 
AATGTGATAC GCTTCACGTT CGAGAAGATG AGCCACCTTG TACAGCTTGG GGGATGTCCC 
CCTGTCCTTT CTAAGGAGAC CATCAGATGG AGGGAAACCG AACCAGACTT CCAGCCAACT 
GATCAAGCCA TTTGTCCAGC ATGGACAAAA GCCGCTGAAT ACTCCAATTG CCATCCACTT 
AGCAACCTGC ATATCAGCAG TCCTGTTATC AATGATCTTG TAGGTGCTGG ACATCCGAAG 
CAAGATAGCA AGGGTCCAGC AGAGTCAGGT CTTTCCCAGA TCCCCACACT GCAATCTGAA 
TTATCCTGCA GTACCAGCAT GTCTGCTAAA ACACCACCCA AGGAAATATA TCTGAAAGGA 
TCGGTGGTGG AGGATATGAA AAACCCTCCT GCACCACCCA GTGATAATGA GAATGAATGC 
AGAGCCCCTC CAGAGGAAGA AATGACACCA ACAGTTGGCA AGTACAGCAT CATCTCTCAG 
GACACTGAGG ACAGCATCAT TGCAAATGCT CAAGAAAAGT ACAATGGTGC CTTGCCTCTG 
AACAGTGAAC TTCAAGTTTG CCCGTCTTTG TTGGGGGATG ATTTGCTGCT TCCTGCTCAA 
TCCACGCTAC CAGAAAAAGA CCCAGACATA TACTGCATTG CAGACTTCGC AGAAGAATTA 
GCAGAGACGA TCGTCTCCAT GGCAACGGAA ATCGCAGCGA TTTGCCTTGA CAACTCCAAT 
GGAAAACAAC CCTGGTTCTG TGCATGGAAA AGAGGGAATG AGTTTCTGGT TACCCCCAAC 
ATCTCCTGCC GATCCTTGAA GAAGAAGAAA GAGAGCCAGG CCAGCGGGGC CACTGTGAGG 
AAACACAAGC CCCCGCGACT CAGCGAGATC AAGAGGAAAA CCGATGAGCA CCCGGAGCTG 
AAAGAGAAGC TGATGAACAG GGTGGTGGAC GAGTCCATGA ACCTCGAGGA CATCCCAGAC 
TCTGTCAGTA TTTTTGCAAA CGAAGTGGCA GCCAAGATCA TGAACCTCAC AGAGTTCTCC 
TTGGTGGACG GTGTGTGGCA AGCACAGGGC TACCCCCGGA GTCGGTTACT GGGTGGGGAC 
AGGTGGAACC GCCTGAAGGC CTCCAGCTGC GAGAGCATTC CAGAGGAGGA CTCTAACACC 
CGGGCCTATG TGAACAGCCT GGGTTTAATG AGTACCCTGA GTCAGCCAGT TAGCCGGGCC 
AGCTCCGTCT CTAAGCAGTC GAGCTGTGAG AGCATCACCG ATGAGTTTTC CAGGTTCATG 
GTGAACCAGA TGGAAAATGA AGGAAGAGGC TTCGAGTTAC TGCTGGATTA CTACGCAGGC 
AAGAATGCCA GCAGCATCTT GAACTCAGCT GTGCAACAGG CCTGCCGGAA AAACGACCAT 
CTGAGTGTGA AGCCAAGCTG TCCCTCGAAG CAGTCCAGCA CCGAAAGCAT CACCGAGGAG 
TTCTACAGGT ACATGCTGAG GGACATTGAA CGAGAGGGCA AAGACGGCCC CTCCTCCAGG 
CAAGGCAGCC AGGACTGGAC CACTGGCTTG CTCTCGCCTT CTCTGCGGTC CCCCTTGTAC 
TACAGACAGT CGTCTGTGCC CGACGGCAGA TCTCCGGGCG CCAGGCTCAC GGTGAACGTG 
CCCATCAAAG CTAACTCCTT AGACGGCTTT TCTCAACACA GCCAGCAAGA CTTCCTCAGC 
GTCCAGCCAG TCGGCGGCGC TTCCTCCTCG GGTCTCTGCA AATCTGACTC TTGCCTGTAT 
CGCAGAGGCG GGGCTGACCA CATCACAAAC ATGTTAATTC ACGAGACGTG GGCAAACTCA 
ATCGAGGCTC TCATGCGGAA GAACAAAATT ATAGTGGATG ATGCCGGCGC GGCTGATGCT 
GAACCTCCTT CAGGGGGCCC CTCCTTGCAA GTGGAAAAGT GTGCGGGCAG ATTAGCTTCG 
GGCAGGGTGC AAAGTGGACC AGCTCTGCTT GTGCAGGAGT CTGTGGATTA TCCGAGGAAA 
AACTCAATCA CAGAAAGCAG CCATCTCCCA GGGTCGTCCC CAAGCAAAGC TGCTCCTCTT 
ACAAGTCACA GCAGTTTAGA TTTTAAAAAG GAAACTCCCT CATGCCACCA TGCGGTCCCT 
CTAAGCCACA CCCGGCGACC ACTTTGCTCA AGGGAAGTGC CTTTAATCCA GATCGAAACA 
GATCAGAGAG AAGAATGTGT GGGAGAACCT GAACACGTTA TTTCCGAACA CAGCCCCCTA 
GAGGAAAAAG AAAGGTGTTT GGAAGAAGAA AAGGTCCCAG AGTCAGGGAA GGGTGAAGAC 
GCTGCCCTGA GTGCCTGCCG GAACCCCAGT GAAACCCTTG ATGCCAGAGA TGTACCGGAG 
GCTGAAGTCT CAAAAGAAGG CAGAGCCCTG GATGAGTTCT CCAGCCCTCC TGGCAGCAGT 
GGGGAGAGCA TGGACAGTTG GTCCCAGCTC GCCAACGAGG AGGACAACCC AGACGACACA 
AGCAGCTTCC TGCAGCTCAG TGAGCGATCC ATGAGCAATG GCAACAGTAG TGCCACTAGC 
AGTCTTGGCA TTATGGACCT GGACATTTAT CAGGAAAGCA TGCCATCTTC TCCCATGATT 
AATGAATTGG TAGAAGAAAA GGAGATTCTT AAAGGACAGG CAGAAACCAT GGAGGAACGT 
GCCTCTGGAA CGCCGGTTGG AACAGCCAGC CGCCAGGGGA GCCTGCTGGT GATCAACTTT 
GACCTGGAGC CAGAGTGTCC TGACACGGAG CTGCGAGCCA CTCTGCAGTG GATAGCTGCA 
TCTGAACTCG GTATCCCCAC CATCTACTTT AAGAAATCTC AAGAAAACAG AATCGAAAAG 
TTTCTAGATG TCGTGCGGCT GGTCCATCAG AAGTCCTGGA AGGTGGGGGA TATCTTCCAT 
GCAGTGGTTC AGTACTGCAA GATACATGAG GAACGGAGGG ATGGGACCCC GAGTCTCTTT 
GATTGGCTCT TGGAACTGGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_005197534.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197591.1 Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

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CloneID	OBa02998
Clone ID Related Accession (Same CDS sequence)	XM_005197534.3 , XM_005203015.3
Accession Version	XM_005197534.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197534.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGCA GCTCCCTGCT CTCCGCACCA AGCAGCTTGG AGTCACCCCT GATGTACCAG 
GTTCCGGAAA CGCAGCAGGG CAGCGCCAGC GCCAGCTCGG GAAGTAGCCT GGGCGGCTCC 
ATCACTGCGT GTAAGAAGGT TCTCCGCAGT AATAGCCTGC TGGAGTCTAC AGACTACTGG 
CTGCAGAATC AGAGAACACC CTGCCAGATT GGTTTCGTGG AAGACAAGTC TGAAAACTGC 
GCTTCTGTCT GCTTTGTGAA CCTGGATGTG AACAAGGATG CATGCAGCAC GGAGCGCCTG 
CAGCAGAAGT TGGTCAATGT TTCACCAGAT CTTCCAAAAC TTATAAGCTC CATGAATGTC 
CAACAGCCAA AAGAAAATGA AATTGTCCTC CTAAGTGGGT TAACATCTGG AAACCTACAG 
GCAGATTTTG AAGTTTCACA GTGTCCTTGG ATGTCAGATG TCTGCTTGGT CCAGTGTGCA 
AGAGGGAACA GACCAAACAG TACCAATTGC ATCATCTTTG AAATCAACAA ATTTTTGATT 
GGTCTGGAAC TGGTACAGGA GCGGCAGCTC CACCTTGAAA CAAACATCTT GAAAGTGGAG 
GATGACACGA ACTGCTCCTT GTCTTCAATT GAAGAAGACT TTCTCACCGC ATCCGAGCAC 
TTGGAGGAGG AAAGTGAAGT GGAAGAGTAT AGGAACGGTT ATGAAAATAT AAATGTCTCA 
ACCAGTATCT TGGAAAATAA AAAGCCAAAG GAAGCCACTA AGGAGGAATG GAATTACAGC 
AAGGAAAAAT TACTTTATAC ATTGGAAGAC AAGTATGTTA GCAAATATCA TCCACCACTG 
GTTAAAACAG AAGGATCTCT GGGAAGAGTG GCAGAATATA CAAGTCTGCA GAGTCTAGAT 
CCTTCAGCCA AGATATCAAA GTGGAAAGGT GAAGCTGTGG GGAATGAGCG GTCAACCACA 
AAGGATTATT CTTCAGAAAA GTGTAAAAGT CAAGAGGAAA ACTCACAGGC GCTATGTAGT 
CCAGGCAATG CTTATTTCTC AAAGATGGTT AAGAGGGATG GACTTTCTGC ATGTGGGACT 
TTCTCTGAGC GTGACAGCAA TTTAGACCCA GGAGACCGTG AAGACCGCAC AAGCTCTCTT 
CTTCCCTTAC AAGATGGAGA AGCCACAACA GGCGAGTATG CCACGAATTT AGCGGAATCT 
GTGATGCAAG ATGCATTTCT TCGACTCTGT AAGTCTCAGC CTGCCCTCCC CCAGGAATCT 
GCAGTCAGTG TTTCTGTAGG AAGTGCTCTG CTTCCCAGTG GCTCTTCCAC AAAAGATATC 
GTGGTACCTC AGTCATGGAA CGAGCCCCCC AAAATCGTCA TTGTCCAGAG TCCAGAAGGC 
AGTGATGCTG GCCCTGAGCC AGGCATCTCC TCGTGGCCCG AGACAGAAGT CTCCAGTGAA 
ACTTCAGGTG TCCCCTCTGG AGAGAACTCC AGAAGACGCC CCCAGAGTCC TCTAGAAGTA 
GCATTAGCTT GTGCAGCCAC TGTTATTGGG ACCATTTCCA GTCCACAAGC CACAGAAAGA 
TTCAAAATGG AGCAAGAACC TCTGGTTTCA CACCATCCAC TGGGAGGTGA TGAGCCAGGA 
CAAACTCAGG CCTCTCAAGT ACTCAAGGAT CCCTCCCTCA GTGAATACTC GTTTCCGTCT 
GCTTTATGTG GCATGACTCA GGTGGCCAGT GCTGTTGCTG TCTGTGGCCT GGGAGAAACA 
GGAGAGGTGA AGTGCCCGGT GGCTTCCAGT GGCTTCCTGT CTGAAGTTGA GGCCCCTGCA 
GCCATCCCCC CACTTTGTGG TTTAGTGGCA GAAAAGAGCA TGGAGCTGGG GAATGAAGCC 
ATTGCAGAGG CCTTGCTCAA GGAGGTTGCC TTGGTCTTAA CAAGACCTAA CGCCTACAGC 
AGCATTGGAG ACCTCATGGA GTCAGTGAAT AGGAGGATCG TAGAAGCTGT TTCAAAGCCT 
CAGACCTTGT GCTCAGAAAA CGCCCTTGGG AATGAACTGG CACAGACCCT GTCTAATGTT 
ATCCTGAAGC ATTCCATTGA TGAAATTCAC CAGAAAAATA AAATGATAGA CCCCAATGAT 
GACAGGGACT CATCTGAAAC CCTGGAATCC TTAATGGAAA GTGTAAATCA GCTGCTCTTC 
AATGTGATAC GCTTCACGTT CGAGAAGATG AGCCACCTTG TACAGCTTGG GGGATGTCCC 
CCTGTCCTTT CTAAGGAGAC CATCAGATGG AGGGAAACCG AACCAGACTT CCAGCCAACT 
GATCAAGCCA TTTGTCCAGC ATGGACAAAA GCCGCTGAAT ACTCCAATTG CCATCCACTT 
AGCAACCTGC ATATCAGCAG TCCTGTTATC AATGATCTTG TAGGTGCTGG ACATCCGAAG 
CAAGATAGCA AGGGTCCAGC AGAGTCAGGT CTTTCCCAGA TCCCCACACT GCAATCTGAA 
TTATCCTGCA GTACCAGCAT GTCTGCTAAA ACACCACCCA AGGAAATATA TCTGAAAGGA 
TCGGTGGTGG AGGATATGAA AAACCCTCCT GCACCACCCA GTGATAATGA GAATGAATGC 
AGAGCCCCTC CAGAGGAAGA AATGACACCA ACAGTTGGCA AGTACAGCAT CATCTCTCAG 
GACACTGAGG ACAGCATCAT TGCAAATGCT CAAGAAAAGT ACAATGGTGC CTTGCCTCTG 
AACAGTGAAC TTCAAGTTTG CCCGTCTTTG TTGGGGGATG ATTTGCTGCT TCCTGCTCAA 
TCCACGCTAC CAGAAAAAGA CCCAGACATA TACTGCATTG CAGACTTCGC AGAAGAATTA 
GCAGAGACGA TCGTCTCCAT GGCAACGGAA ATCGCAGCGA TTTGCCTTGA CAACTCCAAT 
GGAAAACAAC CCTGGTTCTG TGCATGGAAA AGAGGGAATG AGTTTCTGGT TACCCCCAAC 
ATCTCCTGCC GATCCTTGAA GAAGAAGAAA GAGAGCCAGG CCAGCGGGGC CACTGTGAGG 
AAACACAAGC CCCCGCGACT CAGCGAGATC AAGAGGAAAA CCGATGAGCA CCCGGAGCTG 
AAAGAGAAGC TGATGAACAG GGTGGTGGAC GAGTCCATGA ACCTCGAGGA CATCCCAGAC 
TCTGTCAGTA TTTTTGCAAA CGAAGTGGCA GCCAAGATCA TGAACCTCAC AGAGTTCTCC 
TTGGTGGACG GTGTGTGGCA AGCACAGGGC TACCCCCGGA GTCGGTTACT GGGTGGGGAC 
AGGTGGAACC GCCTGAAGGC CTCCAGCTGC GAGAGCATTC CAGAGGAGGA CTCTAACACC 
CGGGCCTATG TGAACAGCCT GGGTTTAATG AGTACCCTGA GTCAGCCAGT TAGCCGGGCC 
AGCTCCGTCT CTAAGCAGTC GAGCTGTGAG AGCATCACCG ATGAGTTTTC CAGGTTCATG 
GTGAACCAGA TGGAAAATGA AGGAAGAGGC TTCGAGTTAC TGCTGGATTA CTACGCAGGC 
AAGAATGCCA GCAGCATCTT GAACTCAGCT GTGCAACAGG CCTGCCGGAA AAACGACCAT 
CTGAGTGTGA AGCCAAGCTG TCCCTCGAAG CAGTCCAGCA CCGAAAGCAT CACCGAGGAG 
TTCTACAGGT ACATGCTGAG GGACATTGAA CGAGAGGGCA AAGACGGCCC CTCCTCCAGG 
CAAGGCAGCC AGGACTGGAC CACTGGCTTG CTCTCGCCTT CTCTGCGGTC CCCCTTGTAC 
TACAGACAGT CGTCTGTGCC CGACGGCAGA TCTCCGGGCG CCAGGCTCAC GGTGAACGTG 
CCCATCAAAG CTAACTCCTT AGACGGCTTT TCTCAACACA GCCAGCAAGA CTTCCTCAGC 
GTCCAGCCAG TCGGCGGCGC TTCCTCCTCG GGTCTCTGCA AATCTGACTC TTGCCTGTAT 
CGCAGAGGCG GGGCTGACCA CATCACAAAC ATGTTAATTC ACGAGACGTG GGCAAACTCA 
ATCGAGGCTC TCATGCGGAA GAACAAAATT ATAGTGGATG ATGCCGGCGC GGCTGATGCT 
GAACCTCCTT CAGGGGGCCC CTCCTTGCAA GTGGAAAAGT GTGCGGGCAG ATTAGCTTCG 
GGCAGGGTGC AAAGTGGACC AGCTCTGCTT GTGCAGGAGT CTGTGGATTA TCCGAGGAAA 
AACTCAATCA CAGAAAGCAG CCATCTCCCA GGGTCGTCCC CAAGCAAAGC TGCTCCTCTT 
ACAAGTCACA GCAGTTTAGA TTTTAAAAAG GAAACTCCCT CATGCCACCA TGCGGTCCCT 
CTAAGCCACA CCCGGCGACC ACTTTGCTCA AGGGAAGTGC CTTTAATCCA GATCGAAACA 
GATCAGAGAG AAGAATGTGT GGGAGAACCT GAACACGTTA TTTCCGAACA CAGCCCCCTA 
GAGGAAAAAG AAAGGTGTTT GGAAGAAGAA AAGGTCCCAG AGTCAGGGAA GGGTGAAGAC 
GCTGCCCTGA GTGCCTGCCG GAACCCCAGT GAAACCCTTG ATGCCAGAGA TGTACCGGAG 
GCTGAAGTCT CAAAAGAAGG CAGAGCCCTG GATGAGTTCT CCAGCCCTCC TGGCAGCAGT 
GGGGAGAGCA TGGACAGTTG GTCCCAGCTC GCCAACGAGG AGGACAACCC AGACGACACA 
AGCAGCTTCC TGCAGCTCAG TGAGCGATCC ATGAGTGAAT TGGTAGAAGA AAAGGAGATT 
CTTAAAGGAC AGGCAGAAAC CATGGAGGAA CGTGCCTCTG GAACGCCGGT TGGAACAGCC 
AGCCGCCAGG GGAGCCTGCT GGTGATCAAC TTTGACCTGG AGCCAGAGTG TCCTGACACG 
GAGCTGCGAG CCACTCTGCA GTGGATAGCT GCATCTGAAC TCGGTATCCC CACCATCTAC 
TTTAAGAAAT CTCAAGAAAA CAGAATCGAA AAGTTTCTAG ATGTCGTGCG GCTGGTCCAT 
CAGAAGTCCT GGAAGGTGGG GGATATCTTC CATGCAGTGG TTCAGTACTG CAAGATACAT 
GAGGAACGGA GGGATGGGAC CCCGAGTCTC TTTGATTGGC TCTTGGAACT GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197591.1
CDS	581..5617
Translation	MDGSSLLSAPSSLESPLMYQVPETQQGSASASSGSSLGGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDKSENCASVCFVNLDVNKDACSTERLQQKLVNVSPDLPKLISSMNVQQPKENEIVLLSGLTSGNLQADFEVSQCPWMSDVCLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKVEDDTNCSLSSIEEDFLTASEHLEEESEVEEYRNGYENINVSTSILENKKPKEATKEEWNYSKEKLLYTLEDKYVSKYHPPLVKTEGSLGRVAEYTSLQSLDPSAKISKWKGEAVGNERSTTKDYSSEKCKSQEENSQALCSPGNAYFSKMVKRDGLSACGTFSERDSNLDPGDREDRTSSLLPLQDGEATTGEYATNLAESVMQDAFLRLCKSQPALPQESAVSVSVGSALLPSGSSTKDIVVPQSWNEPPKIVIVQSPEGSDAGPEPGISSWPETEVSSETSGVPSGENSRRRPQSPLEVALACAATVIGTISSPQATERFKMEQEPLVSHHPLGGDEPGQTQASQVLKDPSLSEYSFPSALCGMTQVASAVAVCGLGETGEVKCPVASSGFLSEVEAPAAIPPLCGLVAEKSMELGNEAIAEALLKEVALVLTRPNAYSSIGDLMESVNRRIVEAVSKPQTLCSENALGNELAQTLSNVILKHSIDEIHQKNKMIDPNDDRDSSETLESLMESVNQLLFNVIRFTFEKMSHLVQLGGCPPVLSKETIRWRETEPDFQPTDQAICPAWTKAAEYSNCHPLSNLHISSPVINDLVGAGHPKQDSKGPAESGLSQIPTLQSELSCSTSMSAKTPPKEIYLKGSVVEDMKNPPAPPSDNENECRAPPEEEMTPTVGKYSIISQDTEDSIIANAQEKYNGALPLNSELQVCPSLLGDDLLLPAQSTLPEKDPDIYCIADFAEELAETIVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLVTPNISCRSLKKKKESQASGATVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDIPDSVSIFANEVAAKIMNLTEFSLVDGVWQAQGYPRSRLLGGDRWNRLKASSCESIPEEDSNTRAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAVQQACRKNDHLSVKPSCPSKQSSTESITEEFYRYMLRDIEREGKDGPSSRQGSQDWTTGLLSPSLRSPLYYRQSSVPDGRSPGARLTVNVPIKANSLDGFSQHSQQDFLSVQPVGGASSSGLCKSDSCLYRRGGADHITNMLIHETWANSIEALMRKNKIIVDDAGAADAEPPSGGPSLQVEKCAGRLASGRVQSGPALLVQESVDYPRKNSITESSHLPGSSPSKAAPLTSHSSLDFKKETPSCHHAVPLSHTRRPLCSREVPLIQIETDQREECVGEPEHVISEHSPLEEKERCLEEEKVPESGKGEDAALSACRNPSETLDARDVPEAEVSKEGRALDEFSSPPGSSGESMDSWSQLANEEDNPDDTSSFLQLSERSMSELVEEKEILKGQAETMEERASGTPVGTASRQGSLLVINFDLEPECPDTELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVRLVHQKSWKVGDIFHAVVQYCKIHEERRDGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_005197534.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197534.3

ORF Insert Sequence:

ATGGATGGCA GCTCCCTGCT CTCCGCACCA AGCAGCTTGG AGTCACCCCT GATGTACCAG 
GTTCCGGAAA CGCAGCAGGG CAGCGCCAGC GCCAGCTCGG GAAGTAGCCT GGGCGGCTCC 
ATCACTGCGT GTAAGAAGGT TCTCCGCAGT AATAGCCTGC TGGAGTCTAC AGACTACTGG 
CTGCAGAATC AGAGAACACC CTGCCAGATT GGTTTCGTGG AAGACAAGTC TGAAAACTGC 
GCTTCTGTCT GCTTTGTGAA CCTGGATGTG AACAAGGATG CATGCAGCAC GGAGCGCCTG 
CAGCAGAAGT TGGTCAATGT TTCACCAGAT CTTCCAAAAC TTATAAGCTC CATGAATGTC 
CAACAGCCAA AAGAAAATGA AATTGTCCTC CTAAGTGGGT TAACATCTGG AAACCTACAG 
GCAGATTTTG AAGTTTCACA GTGTCCTTGG ATGTCAGATG TCTGCTTGGT CCAGTGTGCA 
AGAGGGAACA GACCAAACAG TACCAATTGC ATCATCTTTG AAATCAACAA ATTTTTGATT 
GGTCTGGAAC TGGTACAGGA GCGGCAGCTC CACCTTGAAA CAAACATCTT GAAAGTGGAG 
GATGACACGA ACTGCTCCTT GTCTTCAATT GAAGAAGACT TTCTCACCGC ATCCGAGCAC 
TTGGAGGAGG AAAGTGAAGT GGAAGAGTAT AGGAACGGTT ATGAAAATAT AAATGTCTCA 
ACCAGTATCT TGGAAAATAA AAAGCCAAAG GAAGCCACTA AGGAGGAATG GAATTACAGC 
AAGGAAAAAT TACTTTATAC ATTGGAAGAC AAGTATGTTA GCAAATATCA TCCACCACTG 
GTTAAAACAG AAGGATCTCT GGGAAGAGTG GCAGAATATA CAAGTCTGCA GAGTCTAGAT 
CCTTCAGCCA AGATATCAAA GTGGAAAGGT GAAGCTGTGG GGAATGAGCG GTCAACCACA 
AAGGATTATT CTTCAGAAAA GTGTAAAAGT CAAGAGGAAA ACTCACAGGC GCTATGTAGT 
CCAGGCAATG CTTATTTCTC AAAGATGGTT AAGAGGGATG GACTTTCTGC ATGTGGGACT 
TTCTCTGAGC GTGACAGCAA TTTAGACCCA GGAGACCGTG AAGACCGCAC AAGCTCTCTT 
CTTCCCTTAC AAGATGGAGA AGCCACAACA GGCGAGTATG CCACGAATTT AGCGGAATCT 
GTGATGCAAG ATGCATTTCT TCGACTCTGT AAGTCTCAGC CTGCCCTCCC CCAGGAATCT 
GCAGTCAGTG TTTCTGTAGG AAGTGCTCTG CTTCCCAGTG GCTCTTCCAC AAAAGATATC 
GTGGTACCTC AGTCATGGAA CGAGCCCCCC AAAATCGTCA TTGTCCAGAG TCCAGAAGGC 
AGTGATGCTG GCCCTGAGCC AGGCATCTCC TCGTGGCCCG AGACAGAAGT CTCCAGTGAA 
ACTTCAGGTG TCCCCTCTGG AGAGAACTCC AGAAGACGCC CCCAGAGTCC TCTAGAAGTA 
GCATTAGCTT GTGCAGCCAC TGTTATTGGG ACCATTTCCA GTCCACAAGC CACAGAAAGA 
TTCAAAATGG AGCAAGAACC TCTGGTTTCA CACCATCCAC TGGGAGGTGA TGAGCCAGGA 
CAAACTCAGG CCTCTCAAGT ACTCAAGGAT CCCTCCCTCA GTGAATACTC GTTTCCGTCT 
GCTTTATGTG GCATGACTCA GGTGGCCAGT GCTGTTGCTG TCTGTGGCCT GGGAGAAACA 
GGAGAGGTGA AGTGCCCGGT GGCTTCCAGT GGCTTCCTGT CTGAAGTTGA GGCCCCTGCA 
GCCATCCCCC CACTTTGTGG TTTAGTGGCA GAAAAGAGCA TGGAGCTGGG GAATGAAGCC 
ATTGCAGAGG CCTTGCTCAA GGAGGTTGCC TTGGTCTTAA CAAGACCTAA CGCCTACAGC 
AGCATTGGAG ACCTCATGGA GTCAGTGAAT AGGAGGATCG TAGAAGCTGT TTCAAAGCCT 
CAGACCTTGT GCTCAGAAAA CGCCCTTGGG AATGAACTGG CACAGACCCT GTCTAATGTT 
ATCCTGAAGC ATTCCATTGA TGAAATTCAC CAGAAAAATA AAATGATAGA CCCCAATGAT 
GACAGGGACT CATCTGAAAC CCTGGAATCC TTAATGGAAA GTGTAAATCA GCTGCTCTTC 
AATGTGATAC GCTTCACGTT CGAGAAGATG AGCCACCTTG TACAGCTTGG GGGATGTCCC 
CCTGTCCTTT CTAAGGAGAC CATCAGATGG AGGGAAACCG AACCAGACTT CCAGCCAACT 
GATCAAGCCA TTTGTCCAGC ATGGACAAAA GCCGCTGAAT ACTCCAATTG CCATCCACTT 
AGCAACCTGC ATATCAGCAG TCCTGTTATC AATGATCTTG TAGGTGCTGG ACATCCGAAG 
CAAGATAGCA AGGGTCCAGC AGAGTCAGGT CTTTCCCAGA TCCCCACACT GCAATCTGAA 
TTATCCTGCA GTACCAGCAT GTCTGCTAAA ACACCACCCA AGGAAATATA TCTGAAAGGA 
TCGGTGGTGG AGGATATGAA AAACCCTCCT GCACCACCCA GTGATAATGA GAATGAATGC 
AGAGCCCCTC CAGAGGAAGA AATGACACCA ACAGTTGGCA AGTACAGCAT CATCTCTCAG 
GACACTGAGG ACAGCATCAT TGCAAATGCT CAAGAAAAGT ACAATGGTGC CTTGCCTCTG 
AACAGTGAAC TTCAAGTTTG CCCGTCTTTG TTGGGGGATG ATTTGCTGCT TCCTGCTCAA 
TCCACGCTAC CAGAAAAAGA CCCAGACATA TACTGCATTG CAGACTTCGC AGAAGAATTA 
GCAGAGACGA TCGTCTCCAT GGCAACGGAA ATCGCAGCGA TTTGCCTTGA CAACTCCAAT 
GGAAAACAAC CCTGGTTCTG TGCATGGAAA AGAGGGAATG AGTTTCTGGT TACCCCCAAC 
ATCTCCTGCC GATCCTTGAA GAAGAAGAAA GAGAGCCAGG CCAGCGGGGC CACTGTGAGG 
AAACACAAGC CCCCGCGACT CAGCGAGATC AAGAGGAAAA CCGATGAGCA CCCGGAGCTG 
AAAGAGAAGC TGATGAACAG GGTGGTGGAC GAGTCCATGA ACCTCGAGGA CATCCCAGAC 
TCTGTCAGTA TTTTTGCAAA CGAAGTGGCA GCCAAGATCA TGAACCTCAC AGAGTTCTCC 
TTGGTGGACG GTGTGTGGCA AGCACAGGGC TACCCCCGGA GTCGGTTACT GGGTGGGGAC 
AGGTGGAACC GCCTGAAGGC CTCCAGCTGC GAGAGCATTC CAGAGGAGGA CTCTAACACC 
CGGGCCTATG TGAACAGCCT GGGTTTAATG AGTACCCTGA GTCAGCCAGT TAGCCGGGCC 
AGCTCCGTCT CTAAGCAGTC GAGCTGTGAG AGCATCACCG ATGAGTTTTC CAGGTTCATG 
GTGAACCAGA TGGAAAATGA AGGAAGAGGC TTCGAGTTAC TGCTGGATTA CTACGCAGGC 
AAGAATGCCA GCAGCATCTT GAACTCAGCT GTGCAACAGG CCTGCCGGAA AAACGACCAT 
CTGAGTGTGA AGCCAAGCTG TCCCTCGAAG CAGTCCAGCA CCGAAAGCAT CACCGAGGAG 
TTCTACAGGT ACATGCTGAG GGACATTGAA CGAGAGGGCA AAGACGGCCC CTCCTCCAGG 
CAAGGCAGCC AGGACTGGAC CACTGGCTTG CTCTCGCCTT CTCTGCGGTC CCCCTTGTAC 
TACAGACAGT CGTCTGTGCC CGACGGCAGA TCTCCGGGCG CCAGGCTCAC GGTGAACGTG 
CCCATCAAAG CTAACTCCTT AGACGGCTTT TCTCAACACA GCCAGCAAGA CTTCCTCAGC 
GTCCAGCCAG TCGGCGGCGC TTCCTCCTCG GGTCTCTGCA AATCTGACTC TTGCCTGTAT 
CGCAGAGGCG GGGCTGACCA CATCACAAAC ATGTTAATTC ACGAGACGTG GGCAAACTCA 
ATCGAGGCTC TCATGCGGAA GAACAAAATT ATAGTGGATG ATGCCGGCGC GGCTGATGCT 
GAACCTCCTT CAGGGGGCCC CTCCTTGCAA GTGGAAAAGT GTGCGGGCAG ATTAGCTTCG 
GGCAGGGTGC AAAGTGGACC AGCTCTGCTT GTGCAGGAGT CTGTGGATTA TCCGAGGAAA 
AACTCAATCA CAGAAAGCAG CCATCTCCCA GGGTCGTCCC CAAGCAAAGC TGCTCCTCTT 
ACAAGTCACA GCAGTTTAGA TTTTAAAAAG GAAACTCCCT CATGCCACCA TGCGGTCCCT 
CTAAGCCACA CCCGGCGACC ACTTTGCTCA AGGGAAGTGC CTTTAATCCA GATCGAAACA 
GATCAGAGAG AAGAATGTGT GGGAGAACCT GAACACGTTA TTTCCGAACA CAGCCCCCTA 
GAGGAAAAAG AAAGGTGTTT GGAAGAAGAA AAGGTCCCAG AGTCAGGGAA GGGTGAAGAC 
GCTGCCCTGA GTGCCTGCCG GAACCCCAGT GAAACCCTTG ATGCCAGAGA TGTACCGGAG 
GCTGAAGTCT CAAAAGAAGG CAGAGCCCTG GATGAGTTCT CCAGCCCTCC TGGCAGCAGT 
GGGGAGAGCA TGGACAGTTG GTCCCAGCTC GCCAACGAGG AGGACAACCC AGACGACACA 
AGCAGCTTCC TGCAGCTCAG TGAGCGATCC ATGAGTGAAT TGGTAGAAGA AAAGGAGATT 
CTTAAAGGAC AGGCAGAAAC CATGGAGGAA CGTGCCTCTG GAACGCCGGT TGGAACAGCC 
AGCCGCCAGG GGAGCCTGCT GGTGATCAAC TTTGACCTGG AGCCAGAGTG TCCTGACACG 
GAGCTGCGAG CCACTCTGCA GTGGATAGCT GCATCTGAAC TCGGTATCCC CACCATCTAC 
TTTAAGAAAT CTCAAGAAAA CAGAATCGAA AAGTTTCTAG ATGTCGTGCG GCTGGTCCAT 
CAGAAGTCCT GGAAGGTGGG GGATATCTTC CATGCAGTGG TTCAGTACTG CAAGATACAT 
GAGGAACGGA GGGATGGGAC CCCGAGTCTC TTTGATTGGC TCTTGGAACT GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_005203015.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203072.1 Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

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CloneID	OBa02998
Clone ID Related Accession (Same CDS sequence)	XM_005197534.3 , XM_005203015.3
Accession Version	XM_005203015.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203015.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGCA GCTCCCTGCT CTCCGCACCA AGCAGCTTGG AGTCACCCCT GATGTACCAG 
GTTCCGGAAA CGCAGCAGGG CAGCGCCAGC GCCAGCTCGG GAAGTAGCCT GGGCGGCTCC 
ATCACTGCGT GTAAGAAGGT TCTCCGCAGT AATAGCCTGC TGGAGTCTAC AGACTACTGG 
CTGCAGAATC AGAGAACACC CTGCCAGATT GGTTTCGTGG AAGACAAGTC TGAAAACTGC 
GCTTCTGTCT GCTTTGTGAA CCTGGATGTG AACAAGGATG CATGCAGCAC GGAGCGCCTG 
CAGCAGAAGT TGGTCAATGT TTCACCAGAT CTTCCAAAAC TTATAAGCTC CATGAATGTC 
CAACAGCCAA AAGAAAATGA AATTGTCCTC CTAAGTGGGT TAACATCTGG AAACCTACAG 
GCAGATTTTG AAGTTTCACA GTGTCCTTGG ATGTCAGATG TCTGCTTGGT CCAGTGTGCA 
AGAGGGAACA GACCAAACAG TACCAATTGC ATCATCTTTG AAATCAACAA ATTTTTGATT 
GGTCTGGAAC TGGTACAGGA GCGGCAGCTC CACCTTGAAA CAAACATCTT GAAAGTGGAG 
GATGACACGA ACTGCTCCTT GTCTTCAATT GAAGAAGACT TTCTCACCGC ATCCGAGCAC 
TTGGAGGAGG AAAGTGAAGT GGAAGAGTAT AGGAACGGTT ATGAAAATAT AAATGTCTCA 
ACCAGTATCT TGGAAAATAA AAAGCCAAAG GAAGCCACTA AGGAGGAATG GAATTACAGC 
AAGGAAAAAT TACTTTATAC ATTGGAAGAC AAGTATGTTA GCAAATATCA TCCACCACTG 
GTTAAAACAG AAGGATCTCT GGGAAGAGTG GCAGAATATA CAAGTCTGCA GAGTCTAGAT 
CCTTCAGCCA AGATATCAAA GTGGAAAGGT GAAGCTGTGG GGAATGAGCG GTCAACCACA 
AAGGATTATT CTTCAGAAAA GTGTAAAAGT CAAGAGGAAA ACTCACAGGC GCTATGTAGT 
CCAGGCAATG CTTATTTCTC AAAGATGGTT AAGAGGGATG GACTTTCTGC ATGTGGGACT 
TTCTCTGAGC GTGACAGCAA TTTAGACCCA GGAGACCGTG AAGACCGCAC AAGCTCTCTT 
CTTCCCTTAC AAGATGGAGA AGCCACAACA GGCGAGTATG CCACGAATTT AGCGGAATCT 
GTGATGCAAG ATGCATTTCT TCGACTCTGT AAGTCTCAGC CTGCCCTCCC CCAGGAATCT 
GCAGTCAGTG TTTCTGTAGG AAGTGCTCTG CTTCCCAGTG GCTCTTCCAC AAAAGATATC 
GTGGTACCTC AGTCATGGAA CGAGCCCCCC AAAATCGTCA TTGTCCAGAG TCCAGAAGGC 
AGTGATGCTG GCCCTGAGCC AGGCATCTCC TCGTGGCCCG AGACAGAAGT CTCCAGTGAA 
ACTTCAGGTG TCCCCTCTGG AGAGAACTCC AGAAGACGCC CCCAGAGTCC TCTAGAAGTA 
GCATTAGCTT GTGCAGCCAC TGTTATTGGG ACCATTTCCA GTCCACAAGC CACAGAAAGA 
TTCAAAATGG AGCAAGAACC TCTGGTTTCA CACCATCCAC TGGGAGGTGA TGAGCCAGGA 
CAAACTCAGG CCTCTCAAGT ACTCAAGGAT CCCTCCCTCA GTGAATACTC GTTTCCGTCT 
GCTTTATGTG GCATGACTCA GGTGGCCAGT GCTGTTGCTG TCTGTGGCCT GGGAGAAACA 
GGAGAGGTGA AGTGCCCGGT GGCTTCCAGT GGCTTCCTGT CTGAAGTTGA GGCCCCTGCA 
GCCATCCCCC CACTTTGTGG TTTAGTGGCA GAAAAGAGCA TGGAGCTGGG GAATGAAGCC 
ATTGCAGAGG CCTTGCTCAA GGAGGTTGCC TTGGTCTTAA CAAGACCTAA CGCCTACAGC 
AGCATTGGAG ACCTCATGGA GTCAGTGAAT AGGAGGATCG TAGAAGCTGT TTCAAAGCCT 
CAGACCTTGT GCTCAGAAAA CGCCCTTGGG AATGAACTGG CACAGACCCT GTCTAATGTT 
ATCCTGAAGC ATTCCATTGA TGAAATTCAC CAGAAAAATA AAATGATAGA CCCCAATGAT 
GACAGGGACT CATCTGAAAC CCTGGAATCC TTAATGGAAA GTGTAAATCA GCTGCTCTTC 
AATGTGATAC GCTTCACGTT CGAGAAGATG AGCCACCTTG TACAGCTTGG GGGATGTCCC 
CCTGTCCTTT CTAAGGAGAC CATCAGATGG AGGGAAACCG AACCAGACTT CCAGCCAACT 
GATCAAGCCA TTTGTCCAGC ATGGACAAAA GCCGCTGAAT ACTCCAATTG CCATCCACTT 
AGCAACCTGC ATATCAGCAG TCCTGTTATC AATGATCTTG TAGGTGCTGG ACATCCGAAG 
CAAGATAGCA AGGGTCCAGC AGAGTCAGGT CTTTCCCAGA TCCCCACACT GCAATCTGAA 
TTATCCTGCA GTACCAGCAT GTCTGCTAAA ACACCACCCA AGGAAATATA TCTGAAAGGA 
TCGGTGGTGG AGGATATGAA AAACCCTCCT GCACCACCCA GTGATAATGA GAATGAATGC 
AGAGCCCCTC CAGAGGAAGA AATGACACCA ACAGTTGGCA AGTACAGCAT CATCTCTCAG 
GACACTGAGG ACAGCATCAT TGCAAATGCT CAAGAAAAGT ACAATGGTGC CTTGCCTCTG 
AACAGTGAAC TTCAAGTTTG CCCGTCTTTG TTGGGGGATG ATTTGCTGCT TCCTGCTCAA 
TCCACGCTAC CAGAAAAAGA CCCAGACATA TACTGCATTG CAGACTTCGC AGAAGAATTA 
GCAGAGACGA TCGTCTCCAT GGCAACGGAA ATCGCAGCGA TTTGCCTTGA CAACTCCAAT 
GGAAAACAAC CCTGGTTCTG TGCATGGAAA AGAGGGAATG AGTTTCTGGT TACCCCCAAC 
ATCTCCTGCC GATCCTTGAA GAAGAAGAAA GAGAGCCAGG CCAGCGGGGC CACTGTGAGG 
AAACACAAGC CCCCGCGACT CAGCGAGATC AAGAGGAAAA CCGATGAGCA CCCGGAGCTG 
AAAGAGAAGC TGATGAACAG GGTGGTGGAC GAGTCCATGA ACCTCGAGGA CATCCCAGAC 
TCTGTCAGTA TTTTTGCAAA CGAAGTGGCA GCCAAGATCA TGAACCTCAC AGAGTTCTCC 
TTGGTGGACG GTGTGTGGCA AGCACAGGGC TACCCCCGGA GTCGGTTACT GGGTGGGGAC 
AGGTGGAACC GCCTGAAGGC CTCCAGCTGC GAGAGCATTC CAGAGGAGGA CTCTAACACC 
CGGGCCTATG TGAACAGCCT GGGTTTAATG AGTACCCTGA GTCAGCCAGT TAGCCGGGCC 
AGCTCCGTCT CTAAGCAGTC GAGCTGTGAG AGCATCACCG ATGAGTTTTC CAGGTTCATG 
GTGAACCAGA TGGAAAATGA AGGAAGAGGC TTCGAGTTAC TGCTGGATTA CTACGCAGGC 
AAGAATGCCA GCAGCATCTT GAACTCAGCT GTGCAACAGG CCTGCCGGAA AAACGACCAT 
CTGAGTGTGA AGCCAAGCTG TCCCTCGAAG CAGTCCAGCA CCGAAAGCAT CACCGAGGAG 
TTCTACAGGT ACATGCTGAG GGACATTGAA CGAGAGGGCA AAGACGGCCC CTCCTCCAGG 
CAAGGCAGCC AGGACTGGAC CACTGGCTTG CTCTCGCCTT CTCTGCGGTC CCCCTTGTAC 
TACAGACAGT CGTCTGTGCC CGACGGCAGA TCTCCGGGCG CCAGGCTCAC GGTGAACGTG 
CCCATCAAAG CTAACTCCTT AGACGGCTTT TCTCAACACA GCCAGCAAGA CTTCCTCAGC 
GTCCAGCCAG TCGGCGGCGC TTCCTCCTCG GGTCTCTGCA AATCTGACTC TTGCCTGTAT 
CGCAGAGGCG GGGCTGACCA CATCACAAAC ATGTTAATTC ACGAGACGTG GGCAAACTCA 
ATCGAGGCTC TCATGCGGAA GAACAAAATT ATAGTGGATG ATGCCGGCGC GGCTGATGCT 
GAACCTCCTT CAGGGGGCCC CTCCTTGCAA GTGGAAAAGT GTGCGGGCAG ATTAGCTTCG 
GGCAGGGTGC AAAGTGGACC AGCTCTGCTT GTGCAGGAGT CTGTGGATTA TCCGAGGAAA 
AACTCAATCA CAGAAAGCAG CCATCTCCCA GGGTCGTCCC CAAGCAAAGC TGCTCCTCTT 
ACAAGTCACA GCAGTTTAGA TTTTAAAAAG GAAACTCCCT CATGCCACCA TGCGGTCCCT 
CTAAGCCACA CCCGGCGACC ACTTTGCTCA AGGGAAGTGC CTTTAATCCA GATCGAAACA 
GATCAGAGAG AAGAATGTGT GGGAGAACCT GAACACGTTA TTTCCGAACA CAGCCCCCTA 
GAGGAAAAAG AAAGGTGTTT GGAAGAAGAA AAGGTCCCAG AGTCAGGGAA GGGTGAAGAC 
GCTGCCCTGA GTGCCTGCCG GAACCCCAGT GAAACCCTTG ATGCCAGAGA TGTACCGGAG 
GCTGAAGTCT CAAAAGAAGG CAGAGCCCTG GATGAGTTCT CCAGCCCTCC TGGCAGCAGT 
GGGGAGAGCA TGGACAGTTG GTCCCAGCTC GCCAACGAGG AGGACAACCC AGACGACACA 
AGCAGCTTCC TGCAGCTCAG TGAGCGATCC ATGAGTGAAT TGGTAGAAGA AAAGGAGATT 
CTTAAAGGAC AGGCAGAAAC CATGGAGGAA CGTGCCTCTG GAACGCCGGT TGGAACAGCC 
AGCCGCCAGG GGAGCCTGCT GGTGATCAAC TTTGACCTGG AGCCAGAGTG TCCTGACACG 
GAGCTGCGAG CCACTCTGCA GTGGATAGCT GCATCTGAAC TCGGTATCCC CACCATCTAC 
TTTAAGAAAT CTCAAGAAAA CAGAATCGAA AAGTTTCTAG ATGTCGTGCG GCTGGTCCAT 
CAGAAGTCCT GGAAGGTGGG GGATATCTTC CATGCAGTGG TTCAGTACTG CAAGATACAT 
GAGGAACGGA GGGATGGGAC CCCGAGTCTC TTTGATTGGC TCTTGGAACT GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203072.1
CDS	581..5617
Translation	MDGSSLLSAPSSLESPLMYQVPETQQGSASASSGSSLGGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDKSENCASVCFVNLDVNKDACSTERLQQKLVNVSPDLPKLISSMNVQQPKENEIVLLSGLTSGNLQADFEVSQCPWMSDVCLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKVEDDTNCSLSSIEEDFLTASEHLEEESEVEEYRNGYENINVSTSILENKKPKEATKEEWNYSKEKLLYTLEDKYVSKYHPPLVKTEGSLGRVAEYTSLQSLDPSAKISKWKGEAVGNERSTTKDYSSEKCKSQEENSQALCSPGNAYFSKMVKRDGLSACGTFSERDSNLDPGDREDRTSSLLPLQDGEATTGEYATNLAESVMQDAFLRLCKSQPALPQESAVSVSVGSALLPSGSSTKDIVVPQSWNEPPKIVIVQSPEGSDAGPEPGISSWPETEVSSETSGVPSGENSRRRPQSPLEVALACAATVIGTISSPQATERFKMEQEPLVSHHPLGGDEPGQTQASQVLKDPSLSEYSFPSALCGMTQVASAVAVCGLGETGEVKCPVASSGFLSEVEAPAAIPPLCGLVAEKSMELGNEAIAEALLKEVALVLTRPNAYSSIGDLMESVNRRIVEAVSKPQTLCSENALGNELAQTLSNVILKHSIDEIHQKNKMIDPNDDRDSSETLESLMESVNQLLFNVIRFTFEKMSHLVQLGGCPPVLSKETIRWRETEPDFQPTDQAICPAWTKAAEYSNCHPLSNLHISSPVINDLVGAGHPKQDSKGPAESGLSQIPTLQSELSCSTSMSAKTPPKEIYLKGSVVEDMKNPPAPPSDNENECRAPPEEEMTPTVGKYSIISQDTEDSIIANAQEKYNGALPLNSELQVCPSLLGDDLLLPAQSTLPEKDPDIYCIADFAEELAETIVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLVTPNISCRSLKKKKESQASGATVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDIPDSVSIFANEVAAKIMNLTEFSLVDGVWQAQGYPRSRLLGGDRWNRLKASSCESIPEEDSNTRAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAVQQACRKNDHLSVKPSCPSKQSSTESITEEFYRYMLRDIEREGKDGPSSRQGSQDWTTGLLSPSLRSPLYYRQSSVPDGRSPGARLTVNVPIKANSLDGFSQHSQQDFLSVQPVGGASSSGLCKSDSCLYRRGGADHITNMLIHETWANSIEALMRKNKIIVDDAGAADAEPPSGGPSLQVEKCAGRLASGRVQSGPALLVQESVDYPRKNSITESSHLPGSSPSKAAPLTSHSSLDFKKETPSCHHAVPLSHTRRPLCSREVPLIQIETDQREECVGEPEHVISEHSPLEEKERCLEEEKVPESGKGEDAALSACRNPSETLDARDVPEAEVSKEGRALDEFSSPPGSSGESMDSWSQLANEEDNPDDTSSFLQLSERSMSELVEEKEILKGQAETMEERASGTPVGTASRQGSLLVINFDLEPECPDTELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVRLVHQKSWKVGDIFHAVVQYCKIHEERRDGTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_005203015.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203015.3

ORF Insert Sequence:

ATGGATGGCA GCTCCCTGCT CTCCGCACCA AGCAGCTTGG AGTCACCCCT GATGTACCAG 
GTTCCGGAAA CGCAGCAGGG CAGCGCCAGC GCCAGCTCGG GAAGTAGCCT GGGCGGCTCC 
ATCACTGCGT GTAAGAAGGT TCTCCGCAGT AATAGCCTGC TGGAGTCTAC AGACTACTGG 
CTGCAGAATC AGAGAACACC CTGCCAGATT GGTTTCGTGG AAGACAAGTC TGAAAACTGC 
GCTTCTGTCT GCTTTGTGAA CCTGGATGTG AACAAGGATG CATGCAGCAC GGAGCGCCTG 
CAGCAGAAGT TGGTCAATGT TTCACCAGAT CTTCCAAAAC TTATAAGCTC CATGAATGTC 
CAACAGCCAA AAGAAAATGA AATTGTCCTC CTAAGTGGGT TAACATCTGG AAACCTACAG 
GCAGATTTTG AAGTTTCACA GTGTCCTTGG ATGTCAGATG TCTGCTTGGT CCAGTGTGCA 
AGAGGGAACA GACCAAACAG TACCAATTGC ATCATCTTTG AAATCAACAA ATTTTTGATT 
GGTCTGGAAC TGGTACAGGA GCGGCAGCTC CACCTTGAAA CAAACATCTT GAAAGTGGAG 
GATGACACGA ACTGCTCCTT GTCTTCAATT GAAGAAGACT TTCTCACCGC ATCCGAGCAC 
TTGGAGGAGG AAAGTGAAGT GGAAGAGTAT AGGAACGGTT ATGAAAATAT AAATGTCTCA 
ACCAGTATCT TGGAAAATAA AAAGCCAAAG GAAGCCACTA AGGAGGAATG GAATTACAGC 
AAGGAAAAAT TACTTTATAC ATTGGAAGAC AAGTATGTTA GCAAATATCA TCCACCACTG 
GTTAAAACAG AAGGATCTCT GGGAAGAGTG GCAGAATATA CAAGTCTGCA GAGTCTAGAT 
CCTTCAGCCA AGATATCAAA GTGGAAAGGT GAAGCTGTGG GGAATGAGCG GTCAACCACA 
AAGGATTATT CTTCAGAAAA GTGTAAAAGT CAAGAGGAAA ACTCACAGGC GCTATGTAGT 
CCAGGCAATG CTTATTTCTC AAAGATGGTT AAGAGGGATG GACTTTCTGC ATGTGGGACT 
TTCTCTGAGC GTGACAGCAA TTTAGACCCA GGAGACCGTG AAGACCGCAC AAGCTCTCTT 
CTTCCCTTAC AAGATGGAGA AGCCACAACA GGCGAGTATG CCACGAATTT AGCGGAATCT 
GTGATGCAAG ATGCATTTCT TCGACTCTGT AAGTCTCAGC CTGCCCTCCC CCAGGAATCT 
GCAGTCAGTG TTTCTGTAGG AAGTGCTCTG CTTCCCAGTG GCTCTTCCAC AAAAGATATC 
GTGGTACCTC AGTCATGGAA CGAGCCCCCC AAAATCGTCA TTGTCCAGAG TCCAGAAGGC 
AGTGATGCTG GCCCTGAGCC AGGCATCTCC TCGTGGCCCG AGACAGAAGT CTCCAGTGAA 
ACTTCAGGTG TCCCCTCTGG AGAGAACTCC AGAAGACGCC CCCAGAGTCC TCTAGAAGTA 
GCATTAGCTT GTGCAGCCAC TGTTATTGGG ACCATTTCCA GTCCACAAGC CACAGAAAGA 
TTCAAAATGG AGCAAGAACC TCTGGTTTCA CACCATCCAC TGGGAGGTGA TGAGCCAGGA 
CAAACTCAGG CCTCTCAAGT ACTCAAGGAT CCCTCCCTCA GTGAATACTC GTTTCCGTCT 
GCTTTATGTG GCATGACTCA GGTGGCCAGT GCTGTTGCTG TCTGTGGCCT GGGAGAAACA 
GGAGAGGTGA AGTGCCCGGT GGCTTCCAGT GGCTTCCTGT CTGAAGTTGA GGCCCCTGCA 
GCCATCCCCC CACTTTGTGG TTTAGTGGCA GAAAAGAGCA TGGAGCTGGG GAATGAAGCC 
ATTGCAGAGG CCTTGCTCAA GGAGGTTGCC TTGGTCTTAA CAAGACCTAA CGCCTACAGC 
AGCATTGGAG ACCTCATGGA GTCAGTGAAT AGGAGGATCG TAGAAGCTGT TTCAAAGCCT 
CAGACCTTGT GCTCAGAAAA CGCCCTTGGG AATGAACTGG CACAGACCCT GTCTAATGTT 
ATCCTGAAGC ATTCCATTGA TGAAATTCAC CAGAAAAATA AAATGATAGA CCCCAATGAT 
GACAGGGACT CATCTGAAAC CCTGGAATCC TTAATGGAAA GTGTAAATCA GCTGCTCTTC 
AATGTGATAC GCTTCACGTT CGAGAAGATG AGCCACCTTG TACAGCTTGG GGGATGTCCC 
CCTGTCCTTT CTAAGGAGAC CATCAGATGG AGGGAAACCG AACCAGACTT CCAGCCAACT 
GATCAAGCCA TTTGTCCAGC ATGGACAAAA GCCGCTGAAT ACTCCAATTG CCATCCACTT 
AGCAACCTGC ATATCAGCAG TCCTGTTATC AATGATCTTG TAGGTGCTGG ACATCCGAAG 
CAAGATAGCA AGGGTCCAGC AGAGTCAGGT CTTTCCCAGA TCCCCACACT GCAATCTGAA 
TTATCCTGCA GTACCAGCAT GTCTGCTAAA ACACCACCCA AGGAAATATA TCTGAAAGGA 
TCGGTGGTGG AGGATATGAA AAACCCTCCT GCACCACCCA GTGATAATGA GAATGAATGC 
AGAGCCCCTC CAGAGGAAGA AATGACACCA ACAGTTGGCA AGTACAGCAT CATCTCTCAG 
GACACTGAGG ACAGCATCAT TGCAAATGCT CAAGAAAAGT ACAATGGTGC CTTGCCTCTG 
AACAGTGAAC TTCAAGTTTG CCCGTCTTTG TTGGGGGATG ATTTGCTGCT TCCTGCTCAA 
TCCACGCTAC CAGAAAAAGA CCCAGACATA TACTGCATTG CAGACTTCGC AGAAGAATTA 
GCAGAGACGA TCGTCTCCAT GGCAACGGAA ATCGCAGCGA TTTGCCTTGA CAACTCCAAT 
GGAAAACAAC CCTGGTTCTG TGCATGGAAA AGAGGGAATG AGTTTCTGGT TACCCCCAAC 
ATCTCCTGCC GATCCTTGAA GAAGAAGAAA GAGAGCCAGG CCAGCGGGGC CACTGTGAGG 
AAACACAAGC CCCCGCGACT CAGCGAGATC AAGAGGAAAA CCGATGAGCA CCCGGAGCTG 
AAAGAGAAGC TGATGAACAG GGTGGTGGAC GAGTCCATGA ACCTCGAGGA CATCCCAGAC 
TCTGTCAGTA TTTTTGCAAA CGAAGTGGCA GCCAAGATCA TGAACCTCAC AGAGTTCTCC 
TTGGTGGACG GTGTGTGGCA AGCACAGGGC TACCCCCGGA GTCGGTTACT GGGTGGGGAC 
AGGTGGAACC GCCTGAAGGC CTCCAGCTGC GAGAGCATTC CAGAGGAGGA CTCTAACACC 
CGGGCCTATG TGAACAGCCT GGGTTTAATG AGTACCCTGA GTCAGCCAGT TAGCCGGGCC 
AGCTCCGTCT CTAAGCAGTC GAGCTGTGAG AGCATCACCG ATGAGTTTTC CAGGTTCATG 
GTGAACCAGA TGGAAAATGA AGGAAGAGGC TTCGAGTTAC TGCTGGATTA CTACGCAGGC 
AAGAATGCCA GCAGCATCTT GAACTCAGCT GTGCAACAGG CCTGCCGGAA AAACGACCAT 
CTGAGTGTGA AGCCAAGCTG TCCCTCGAAG CAGTCCAGCA CCGAAAGCAT CACCGAGGAG 
TTCTACAGGT ACATGCTGAG GGACATTGAA CGAGAGGGCA AAGACGGCCC CTCCTCCAGG 
CAAGGCAGCC AGGACTGGAC CACTGGCTTG CTCTCGCCTT CTCTGCGGTC CCCCTTGTAC 
TACAGACAGT CGTCTGTGCC CGACGGCAGA TCTCCGGGCG CCAGGCTCAC GGTGAACGTG 
CCCATCAAAG CTAACTCCTT AGACGGCTTT TCTCAACACA GCCAGCAAGA CTTCCTCAGC 
GTCCAGCCAG TCGGCGGCGC TTCCTCCTCG GGTCTCTGCA AATCTGACTC TTGCCTGTAT 
CGCAGAGGCG GGGCTGACCA CATCACAAAC ATGTTAATTC ACGAGACGTG GGCAAACTCA 
ATCGAGGCTC TCATGCGGAA GAACAAAATT ATAGTGGATG ATGCCGGCGC GGCTGATGCT 
GAACCTCCTT CAGGGGGCCC CTCCTTGCAA GTGGAAAAGT GTGCGGGCAG ATTAGCTTCG 
GGCAGGGTGC AAAGTGGACC AGCTCTGCTT GTGCAGGAGT CTGTGGATTA TCCGAGGAAA 
AACTCAATCA CAGAAAGCAG CCATCTCCCA GGGTCGTCCC CAAGCAAAGC TGCTCCTCTT 
ACAAGTCACA GCAGTTTAGA TTTTAAAAAG GAAACTCCCT CATGCCACCA TGCGGTCCCT 
CTAAGCCACA CCCGGCGACC ACTTTGCTCA AGGGAAGTGC CTTTAATCCA GATCGAAACA 
GATCAGAGAG AAGAATGTGT GGGAGAACCT GAACACGTTA TTTCCGAACA CAGCCCCCTA 
GAGGAAAAAG AAAGGTGTTT GGAAGAAGAA AAGGTCCCAG AGTCAGGGAA GGGTGAAGAC 
GCTGCCCTGA GTGCCTGCCG GAACCCCAGT GAAACCCTTG ATGCCAGAGA TGTACCGGAG 
GCTGAAGTCT CAAAAGAAGG CAGAGCCCTG GATGAGTTCT CCAGCCCTCC TGGCAGCAGT 
GGGGAGAGCA TGGACAGTTG GTCCCAGCTC GCCAACGAGG AGGACAACCC AGACGACACA 
AGCAGCTTCC TGCAGCTCAG TGAGCGATCC ATGAGTGAAT TGGTAGAAGA AAAGGAGATT 
CTTAAAGGAC AGGCAGAAAC CATGGAGGAA CGTGCCTCTG GAACGCCGGT TGGAACAGCC 
AGCCGCCAGG GGAGCCTGCT GGTGATCAAC TTTGACCTGG AGCCAGAGTG TCCTGACACG 
GAGCTGCGAG CCACTCTGCA GTGGATAGCT GCATCTGAAC TCGGTATCCC CACCATCTAC 
TTTAAGAAAT CTCAAGAAAA CAGAATCGAA AAGTTTCTAG ATGTCGTGCG GCTGGTCCAT 
CAGAAGTCCT GGAAGGTGGG GGATATCTTC CATGCAGTGG TTCAGTACTG CAAGATACAT 
GAGGAACGGA GGGATGGGAC CCCGAGTCTC TTTGATTGGC TCTTGGAACT GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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SPHKAP cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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