SPHKAP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPHKAP
Entrez Gene ID	80309
Full Name	SPHK1 interactor, AKAP domain containing
Synonyms	SKIP
General protein information	Preferred Names SPHK1 interactor, AKAP domain containing Names A-kinase anchor protein SPHKAP SPHK1 (sphingosine kinase type 1) interacting protein SPHK1-interactor and AKAP domain-containing protein sphingosine kinase type 1-interacting protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q36.3
Disorder MIM:	611646

mRNA and Protein(s)

mRNA	Protein	Name
XM_006712778.3	XP_006712841.1	A-kinase anchor protein SPHKAP isoform X3
XM_006712778.4	XP_006712841.1	A-kinase anchor protein SPHKAP isoform X3
XM_005246870.4	XP_005246927.1	A-kinase anchor protein SPHKAP isoform X4
XM_006712777.3	XP_006712840.1	A-kinase anchor protein SPHKAP isoform X1
NM_030623.3	NP_085126.2	A-kinase anchor protein SPHKAP isoform 2
NM_001142644.2	NP_001136116.1	A-kinase anchor protein SPHKAP isoform 1
XM_011511925.2	XP_011510227.1	A-kinase anchor protein SPHKAP isoform X2
NM_001142644.1	NP_001136116.1	A-kinase anchor protein SPHKAP isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPHKAP	XP_005635852.1
Gallus gallus (chicken)	SPHKAP	NP_001171003.1
Xenopus tropicalis (tropical clawed frog)	sphkap	XP_002933413.2
Bos taurus (cattle)	SPHKAP	XP_002685689.2
Mus musculus (house mouse)	Sphkap	NP_766018.3
Danio rerio (zebrafish)	sphkap	NP_001076514.1
Homo sapiens (human)	SPHKAP	NP_001136116.1
Pan troglodytes (chimpanzee)	SPHKAP	XP_001137841.1
	SPHKAP	XP_001111587.1
Rattus norvegicus (Norway rat)	Sphkap	NP_001120964.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic variants associated with disordered eating.
Wade TD, Gordon S, Medland S, Bulik CM, Heath AC, Montgomery GW, Martin NG
The International journal of eating disorders46(6)594-608(2013 Sep)

Genome wide analysis of acute myeloid leukemia reveal leukemia specific methylome and subtype specific hypomethylation of repeats.
Saied MH, Marzec J, Khalid S, Smith P, Down TA, Rakyan VK, Molloy G, Raghavan M, Debernardi S, Young BD
PloS one7(3)e33213(2012)

An entirely specific type I A-kinase anchoring protein that can sequester two molecules of protein kinase A at mitochondria.
Means CK, Lygren B, Langeberg LK, Jain A, Dixon RE, Vega AL, Gold MG, Petrosyan S, Taylor SS, Murphy AN, Ha T, Santana LF, Tasken K, Scott JD
Proceedings of the National Academy of Sciences of the United States of America108(48)E1227-35(2011 Nov)

Sphingosine kinase interacting protein is an A-kinase anchoring protein specific for type I cAMP-dependent protein kinase.
Kovanich D, van der Heyden MA, Aye TT, van Veen TA, Heck AJ, Scholten A
Chembiochem : a European journal of chemical biology11(7)963-71(2010 May)

Sphingosine kinase 1 is up-regulated during hypoxia in U87MG glioma cells. Role of hypoxia-inducible factors 1 and 2.
Anelli V, Gault CR, Cheng AB, Obeid LM
The Journal of biological chemistry283(6)3365-75(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results showed that DPP6, SPHKAP and ID4 were down regulated in acute myeloid leukemia (AML) patients.
Title: Genome wide analysis of acute myeloid leukemia reveal leukemia specific methylome and subtype specific hypomethylation of repeats.

SKIP is an entirely specific type I A-kinase anchoring protein that can sequester two molecules of protein kinase A at mitochondria.
Title: An entirely specific type I A-kinase anchoring protein that can sequester two molecules of protein kinase A at mitochondria.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

sphingosine kinase type 1-interacting protein can be considered as the first mammalian A-kinase-anchoring proteins that preferentially binds to regulatory subunit 1 of cAMP-dependent protein kinase.
Title: Sphingosine kinase interacting protein is an A-kinase anchoring protein specific for type I cAMP-dependent protein kinase.

HIF-1 and HIF-2 have roles in SK1 upregulation during hypoxia in glioma cells
Title: Sphingosine kinase 1 is up-regulated during hypoxia in U87MG glioma cells. Role of hypoxia-inducible factors 1 and 2.

The following SPHKAP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPHKAP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09151	NM_001142644.2 Latest version!	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu36652	XM_005246870.4 Latest version!	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$951.30 $1359.00
OHu09199	NM_030623.3 Latest version!	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu36648	XM_006712777.3 Latest version!	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu36650	XM_006712778.4 Latest version!	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu72743	XM_011511925.2 Latest version!	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu36650	XM_006712778.3	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu09151	NM_001142644.1	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPHKAP ( NM_001142644.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136116.1 Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09151
Clone ID Related Accession (Same CDS sequence)	NM_001142644.2 , NM_001142644.1
Accession Version	NM_001142644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	A-kinase anchor protein SPHKAP isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109825.4, BC104956.1, AB051465.1 and AK095042.1. On Nov 23, 2018 this sequence version replaced NM_001142644.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803617.250221.1, SRR1660807.220106.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000392056.8/ ENSP00000375909.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATGGCA ACTCCCTGCT CTCGGTACCA AGCAACTTGG AGTCATCACG GATGTATGAC 
GTTTTGGAAC CGCAGCAGGG CAGAGGCTGT GGCAGCTCAG GAAGCGGCCC GGGGAACTCC 
ATCACAGCCT GTAAGAAGGT TCTTCGCAGC AATAGCCTGC TGGAGTCAAC AGACTACTGG 
TTGCAGAATC AGAGGATGCC CTGCCAAATT GGTTTTGTAG AAGACAAGTC TGAAAACTGT 
GCTTCTGTCT GCTTTGTGAA TCTTGATGTG AACAAGGATG AATGCAGCAC AGAGCACCTG 
CAACAGAAAC TGGTCAACGT TTCACCAGAT CTTCCAAAAC TTATCAGTTC CATGAATGTC 
CAACAACCAA AAGAAAATGA AATTGTTGTC CTAAGTGGGT TAGCCTCTGG AAATCTCCAG 
GCAGATTTTG AAGTTTCACA GTGCCCTTGG CTGCCAGATA TCTGCTTGGT CCAATGTGCA 
AGAGGGAACA GACCAAACAG TACCAACTGC ATCATCTTTG AAATCAACAA ATTTCTGATT 
GGTCTGGAAC TGGTGCAGGA GCGACAGCTC CACCTGGAAA CAAACATCTT GAAACTGGAG 
GATGACACGA ACTGTTCCTT ATCTTCAATC GAGGAAGACT TTCTCACCGC TTCTGAGCAC 
TTGGAGGAGG AAAGCGAGGT GGATGAATCT AGGAACGATT ATGAAAATAT AAATGTCTCA 
GCCAATGTTT TGGAAAGTAA ACAGCTAAAG GGAGCCACCC AGGTGGAATG GAATTGCAAC 
AAGGAAAAGT GGCTTTATGC TTTGGAAGAC AAATACATCA ACAAATATCC CACACCATTG 
ATTAAAACAG AACGATCTCC AGAAAACCTA ACAAAGAACA CAGCCTTGCA GAGTCTAGAT 
CCCTCAGCCA AGCCATCACA GTGGAAAAGA GAAGCTGTGG GGAATGGGAG ACAAGCCACA 
CATTATTATC ATTCAGAAGC TTTTAAAGGT CAAATGGAAA AATCACAGGC ACTGTATATT 
CCAAAAGATG CTTATTTCTC CATGATGGAT AAAGATGTAC CTTCTGCATG TGCTGTGGCA 
GAGCAGAGAA GCAACCTAAA CCCAGGAGAC CATGAAGACA CAAGAAACGC TCTCCCTCCT 
AGACAAGATG GAGAAGTCAC CACTGGCAAG TATGCTACAA ATTTAGCAGA ATCCGTGCTG 
CAGGATGCAT TTATTAGATT ATCTCAATCT CAGTCCACAT TACCCCAGGA ATCTGCAGTC 
AGTGTTTCTG TAGGAAGTTC TCTGCTTCCC AGTTGCTATT CCACAAAAGA TACAGTGGTT 
TCTCGGTCAT GGAATGAGCT CCCCAAAATC GTCGTTGTTC AGAGTCCAGA TGGCAGTGAT 
GCTGCCCCAC AGCCAGGCAT CTCCTCCTGG CCTGAGATGG AAGTCTCTGT TGAAACCTCA 
AGCATCCTCT CTGGAGAGAA CTCCAGCAGA CAACCCCAGA GTGCTCTAGA AGTGGCGTTA 
GCTTGTGCAG CCACTGTGAT TGGAACTATT TCCAGTCCAC AGGCCACAGA AAGACTCAAA 
ATGGAGCAAG TGGTCTCGAA CTTTCCCCCA GGGAGCAGTG GTGCACTGCA AACTCAAGCA 
CCCCAAGGAC TCAAGGAACC TTCCATCAAT GAGTACTCCT TTCCATCTGC TTTGTGTGGC 
ATGACTCAGG TGGCCAGTGC CGTGGCTGTC TGTGGTCTGG GTGAAAGAGA AGAGGTGACA 
TGCTCAGTGG CTCCAAGTGG TAGCCTCCCG CCTGCAGCTG AGGCTTCTGA AGCCATGCCC 
CCACTTTGTG GTTTAGCAAG CATGGAGCTT GGCAAGGAAG CCATTGCCAA GGGATTGCTC 
AAGGAGGCTG CTCTGGTTTT AACAAGGCCT AATACCTACA GCAGCATTGG AGACTTTCTG 
GACTCCATGA ACAGGAGAAT CATGGAAACT GCTTCAAAGT CTCAGACCCT GTGCTCAGAA 
AATGTCGTCA GGAATGAACT GGCACATACC CTGTCCAATG TTATCCTGAG GCATTCCATT 
GATGAAGTTC ACCACAAAAA TATGATAATC GACCCCAATG ACAACAGGCA TTCATCTGAA 
ATTCTGGACA CCTTAATGGA AAGTACAAAT CAACTGCTTT TAGATGTGAT ATGCTTCACG 
TTCAAGAAGA TGAGTCATAT TGTACGGCTT GGTGAATGTC CTGCTGTCCT TTCTAAGGAG 
ACCATCAGAA GGAGGGAGAC AGAACCAAGC TGCCAGCCAT CTGATCCGGG TGCTAGTCAA 
GCTTGGACAA AAGCCACTGA ATCCTCCAGC AGCTCTCCAC TTAGCAATTC ACACAACACG 
AGTCTTGTCA TCAACAATCT TGTGGATGGC ATGTATTCAA AACAAGACAA GGGTGGAGTG 
AGGCCAGGCC TCTTCAAGAA CCCCACGCTG CAGTCACAAT TATCACGTAG TCACAGAGTG 
CCCGATTCTT CAACTGCTAC AACATCCTCC AAGGAAATAT ATCTGAAAGG AATAGCAGGA 
GAGGATACAA AAAGCCCTCA TCACAGTGAG AATGAATGCA GAGCCTCTTC CGAAGGACAA 
AGGTCCCCAA CGGTCAGCCA GTCCAGAAGT GGTTCCCAGG AGGCTGAGGA GAGTATCCAC 
CCAAACACCC AAGAAAAGTA CAACTGTGCC ACATCTCGCA TCAACGAAGT TCAAGTCAAC 
CTGTCCTTGT TAGGGGATGA CCTGCTGCTT CCTGCTCAAT CCACGCTTCA AACAAAGCAT 
CCAGACATCT ACTGCATTAC AGACTTTGCG GAAGAATTAG CAGACACGGT CGTCTCCATG 
GCAACTGAAA TTGCAGCGAT TTGCCTTGAC AACTCCAGTG GAAAACAACC CTGGTTTTGT 
GCATGGAAAA GAGGGAGTGA GTTTCTGATG ACACCCAACG TACCCTGCCG ATCCTTGAAG 
AGGAAGAAAG AGAGCCAGGG GAGCGGGACC GCTGTGAGGA AACACAAGCC TCCCCGGCTC 
AGTGAGATCA AGAGGAAGAC GGACGAGCAC CCTGAGCTTA AAGAAAAGCT GATGAACAGG 
GTTGTGGATG AGTCCATGAA CCTTGAAGAT GTCCCAGATT CTGTCAATCT TTTTGCCAAT 
GAAGTGGCAG CCAAGATCAT GAACCTAACG GAGTTCTCTA TGGTGGACGG CATGTGGCAG 
GCGCAGGGCT ATCCCCGGAA TCGGTTACTG AGTGGCGACA GGTGGAGCCG GCTGAAGGCC 
TCCAGCTGCG AAAGCATTCC TGAGGAAGAC TCCGAGGCCA GGGCCTATGT CAACAGCCTG 
GGCTTAATGA GCACGCTGAG CCAGCCGGTC AGCAGGGCCA GCTCTGTCTC CAAGCAGTCG 
AGCTGTGAGA GCATCACCGA TGAGTTTTCC AGGTTCATGG TGAACCAGAT GGAAAATGAA 
GGGAGAGGAT TTGAGTTACT GCTGGATTAC TATGCTGGCA AGAACGCCAG CAGCATTCTG 
AACTCAGCCA TGCAACAGGC GTGCCGGAAA AGTGACCACC TCAGTGTGAG GCCTAGCTGT 
CCCTCTAAGC AGTCCAGCAC AGAGAGCATC ACTGAGGAGT TCTACAGGTA CATGCTGAGG 
GACATCGAAA GAGACAGCAG AGAAAGTGCC TCCTCCAGAC GGAGCAGCCA GGATTGGACA 
GCCGGCCTGC TGTCTCCTTC TCTGCGATCC CCAGTGTGCC ACAGACAGTC GTCCATGCCA 
GACAGCAGAT CCCCATGCTC CAGGCTGACA GTGAATGTGC CCATCAAAGC CAACTCTTTA 
GATGGCTTTG CTCAGAACTG CCCACAAGAT TTCCTAAGCG TGCAGCCGGT CAGTAGCGCG 
TCCTCATCCG GTCTCTGCAA ATCTGACTCT TGCTTGTATC GGAGAGGTGG GACTGACCAC 
ATCACCAACA TGTTAATTCA TGAAACGTGG GCTAGCTCCA TTGAGGCTCT CATGCGCAAG 
AACAAAATCA TTGTGGATGA TGCAGAGGAA GCTGACACTG AGCCTGTTTC TGGTGGCTCT 
CCCTCGCAAG CAGAGAAGTG TGCAAATAGA TTAGCTGCGA GCAGGATGTG CAGTGGGCCA 
ACTCTGCTTG TTCAGGAGTC TCTCGATTGC CCGAGGAAAG ACTCTGTTAC CGAATGTAAA 
CAGCCCCCAG TGTCATCTTT GAGCAAAACT GCTTCTCTTA CAAACCACAG CCCTTTAGAT 
TCTAAAAAAG AAACTTCCTC GTGCCAGGAC CCTGTACCAA TAAACCACAA AAGGCGATCA 
CTTTGCTCGA GGGAAGTGCC TTTGATTCAG ATTGAAACAG ATCAGAGAGA AGCCTGTGCT 
GGGGAACCTG AACCCTTCCT TTCCAAAAGC AGCCTCCTAG AGGAAGCAGA AGGGCATTCG 
AATGACAAAA ACATCCCAGA TGTGGTGAGA GGTGGAGACA CAGCCGTGAG CGCTTGTCAA 
ATCCATAGTG ACAGCCTTGA TACCAGAGAT GTACCAGAGG CTGAAGCCTC CACAGAAGCC 
AGAGCCCCCG ATGAGGCCCC CAACCCTCCA AGCAGCAGCG AGGAGAGCAC AGGCAGCTGG 
ACCCAGCTTG CCAATGAGGA AGACAACCCA GATGACACAA GTAGCTTTCT CCAGCTCAGT 
GAGCGATCCA TGAGCAATGG CAACAGTAGT GCCACTAGCA GTCTTGGCAT TATGGATCTG 
GACATTTATC AGGAAAGCAT GCCATCTTCT CCCATGATTA ATGAATTAGT AGAAGAAAAG 
AAGATTCTTA AAGGACAGTC AGAAAGCACA GAGGCACCTG CATCTGGACC GCCTACGGGA 
ACAGCCAGCC CCCAGAGGAG CCTGCTGGTG ATCAACTTTG ACCTGGAGCC AGAGTGTCCA 
GATGCCGAGC TCCGAGCCAC TCTGCAGTGG ATAGCTGCCT CTGAACTGGG GATTCCCACC 
ATCTACTTTA AGAAATCTCA GGAAAACAGA ATTGAAAAGT TTCTAGATGT CGTGCAGCTG 
GTTCATCGGA AGTCCTGGAA AGTGGGTGAT ATCTTCCATG CAGTTGTCCA GTACTGCAAA 
ATGCATGAGG AGCAGAAGGA TGGGAGACTG AGTCTCTTTG ACTGGCTCTT GGAACTGGGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136116.1
CDS	90..5192
Translation	MDGNSLLSVPSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQIGFVEDKSENCASVCFVNLDVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSIEEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPLIKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMDKDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAVSVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSILSGENSSRQPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCGMTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRPNTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSEILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSSSSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAGEDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLLPAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLKRKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLTEFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERDSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSLDGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEEADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLDSKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVRGGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLSERSMSNGNSSATSSLGIMDLDIYQESMPSSPMINELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCKMHEEQKDGRLSLFDWLLELG

Target ORF information:

RefSeq Version	NM_001142644.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142644.2

ORF Insert Sequence:

ATGGATGGCA ACTCCCTGCT CTCGGTACCA AGCAACTTGG AGTCATCACG GATGTATGAC 
GTTTTGGAAC CGCAGCAGGG CAGAGGCTGT GGCAGCTCAG GAAGCGGCCC GGGGAACTCC 
ATCACAGCCT GTAAGAAGGT TCTTCGCAGC AATAGCCTGC TGGAGTCAAC AGACTACTGG 
TTGCAGAATC AGAGGATGCC CTGCCAAATT GGTTTTGTAG AAGACAAGTC TGAAAACTGT 
GCTTCTGTCT GCTTTGTGAA TCTTGATGTG AACAAGGATG AATGCAGCAC AGAGCACCTG 
CAACAGAAAC TGGTCAACGT TTCACCAGAT CTTCCAAAAC TTATCAGTTC CATGAATGTC 
CAACAACCAA AAGAAAATGA AATTGTTGTC CTAAGTGGGT TAGCCTCTGG AAATCTCCAG 
GCAGATTTTG AAGTTTCACA GTGCCCTTGG CTGCCAGATA TCTGCTTGGT CCAATGTGCA 
AGAGGGAACA GACCAAACAG TACCAACTGC ATCATCTTTG AAATCAACAA ATTTCTGATT 
GGTCTGGAAC TGGTGCAGGA GCGACAGCTC CACCTGGAAA CAAACATCTT GAAACTGGAG 
GATGACACGA ACTGTTCCTT ATCTTCAATC GAGGAAGACT TTCTCACCGC TTCTGAGCAC 
TTGGAGGAGG AAAGCGAGGT GGATGAATCT AGGAACGATT ATGAAAATAT AAATGTCTCA 
GCCAATGTTT TGGAAAGTAA ACAGCTAAAG GGAGCCACCC AGGTGGAATG GAATTGCAAC 
AAGGAAAAGT GGCTTTATGC TTTGGAAGAC AAATACATCA ACAAATATCC CACACCATTG 
ATTAAAACAG AACGATCTCC AGAAAACCTA ACAAAGAACA CAGCCTTGCA GAGTCTAGAT 
CCCTCAGCCA AGCCATCACA GTGGAAAAGA GAAGCTGTGG GGAATGGGAG ACAAGCCACA 
CATTATTATC ATTCAGAAGC TTTTAAAGGT CAAATGGAAA AATCACAGGC ACTGTATATT 
CCAAAAGATG CTTATTTCTC CATGATGGAT AAAGATGTAC CTTCTGCATG TGCTGTGGCA 
GAGCAGAGAA GCAACCTAAA CCCAGGAGAC CATGAAGACA CAAGAAACGC TCTCCCTCCT 
AGACAAGATG GAGAAGTCAC CACTGGCAAG TATGCTACAA ATTTAGCAGA ATCCGTGCTG 
CAGGATGCAT TTATTAGATT ATCTCAATCT CAGTCCACAT TACCCCAGGA ATCTGCAGTC 
AGTGTTTCTG TAGGAAGTTC TCTGCTTCCC AGTTGCTATT CCACAAAAGA TACAGTGGTT 
TCTCGGTCAT GGAATGAGCT CCCCAAAATC GTCGTTGTTC AGAGTCCAGA TGGCAGTGAT 
GCTGCCCCAC AGCCAGGCAT CTCCTCCTGG CCTGAGATGG AAGTCTCTGT TGAAACCTCA 
AGCATCCTCT CTGGAGAGAA CTCCAGCAGA CAACCCCAGA GTGCTCTAGA AGTGGCGTTA 
GCTTGTGCAG CCACTGTGAT TGGAACTATT TCCAGTCCAC AGGCCACAGA AAGACTCAAA 
ATGGAGCAAG TGGTCTCGAA CTTTCCCCCA GGGAGCAGTG GTGCACTGCA AACTCAAGCA 
CCCCAAGGAC TCAAGGAACC TTCCATCAAT GAGTACTCCT TTCCATCTGC TTTGTGTGGC 
ATGACTCAGG TGGCCAGTGC CGTGGCTGTC TGTGGTCTGG GTGAAAGAGA AGAGGTGACA 
TGCTCAGTGG CTCCAAGTGG TAGCCTCCCG CCTGCAGCTG AGGCTTCTGA AGCCATGCCC 
CCACTTTGTG GTTTAGCAAG CATGGAGCTT GGCAAGGAAG CCATTGCCAA GGGATTGCTC 
AAGGAGGCTG CTCTGGTTTT AACAAGGCCT AATACCTACA GCAGCATTGG AGACTTTCTG 
GACTCCATGA ACAGGAGAAT CATGGAAACT GCTTCAAAGT CTCAGACCCT GTGCTCAGAA 
AATGTCGTCA GGAATGAACT GGCACATACC CTGTCCAATG TTATCCTGAG GCATTCCATT 
GATGAAGTTC ACCACAAAAA TATGATAATC GACCCCAATG ACAACAGGCA TTCATCTGAA 
ATTCTGGACA CCTTAATGGA AAGTACAAAT CAACTGCTTT TAGATGTGAT ATGCTTCACG 
TTCAAGAAGA TGAGTCATAT TGTACGGCTT GGTGAATGTC CTGCTGTCCT TTCTAAGGAG 
ACCATCAGAA GGAGGGAGAC AGAACCAAGC TGCCAGCCAT CTGATCCGGG TGCTAGTCAA 
GCTTGGACAA AAGCCACTGA ATCCTCCAGC AGCTCTCCAC TTAGCAATTC ACACAACACG 
AGTCTTGTCA TCAACAATCT TGTGGATGGC ATGTATTCAA AACAAGACAA GGGTGGAGTG 
AGGCCAGGCC TCTTCAAGAA CCCCACGCTG CAGTCACAAT TATCACGTAG TCACAGAGTG 
CCCGATTCTT CAACTGCTAC AACATCCTCC AAGGAAATAT ATCTGAAAGG AATAGCAGGA 
GAGGATACAA AAAGCCCTCA TCACAGTGAG AATGAATGCA GAGCCTCTTC CGAAGGACAA 
AGGTCCCCAA CGGTCAGCCA GTCCAGAAGT GGTTCCCAGG AGGCTGAGGA GAGTATCCAC 
CCAAACACCC AAGAAAAGTA CAACTGTGCC ACATCTCGCA TCAACGAAGT TCAAGTCAAC 
CTGTCCTTGT TAGGGGATGA CCTGCTGCTT CCTGCTCAAT CCACGCTTCA AACAAAGCAT 
CCAGACATCT ACTGCATTAC AGACTTTGCG GAAGAATTAG CAGACACGGT CGTCTCCATG 
GCAACTGAAA TTGCAGCGAT TTGCCTTGAC AACTCCAGTG GAAAACAACC CTGGTTTTGT 
GCATGGAAAA GAGGGAGTGA GTTTCTGATG ACACCCAACG TACCCTGCCG ATCCTTGAAG 
AGGAAGAAAG AGAGCCAGGG GAGCGGGACC GCTGTGAGGA AACACAAGCC TCCCCGGCTC 
AGTGAGATCA AGAGGAAGAC GGACGAGCAC CCTGAGCTTA AAGAAAAGCT GATGAACAGG 
GTTGTGGATG AGTCCATGAA CCTTGAAGAT GTCCCAGATT CTGTCAATCT TTTTGCCAAT 
GAAGTGGCAG CCAAGATCAT GAACCTAACG GAGTTCTCTA TGGTGGACGG CATGTGGCAG 
GCGCAGGGCT ATCCCCGGAA TCGGTTACTG AGTGGCGACA GGTGGAGCCG GCTGAAGGCC 
TCCAGCTGCG AAAGCATTCC TGAGGAAGAC TCCGAGGCCA GGGCCTATGT CAACAGCCTG 
GGCTTAATGA GCACGCTGAG CCAGCCGGTC AGCAGGGCCA GCTCTGTCTC CAAGCAGTCG 
AGCTGTGAGA GCATCACCGA TGAGTTTTCC AGGTTCATGG TGAACCAGAT GGAAAATGAA 
GGGAGAGGAT TTGAGTTACT GCTGGATTAC TATGCTGGCA AGAACGCCAG CAGCATTCTG 
AACTCAGCCA TGCAACAGGC GTGCCGGAAA AGTGACCACC TCAGTGTGAG GCCTAGCTGT 
CCCTCTAAGC AGTCCAGCAC AGAGAGCATC ACTGAGGAGT TCTACAGGTA CATGCTGAGG 
GACATCGAAA GAGACAGCAG AGAAAGTGCC TCCTCCAGAC GGAGCAGCCA GGATTGGACA 
GCCGGCCTGC TGTCTCCTTC TCTGCGATCC CCAGTGTGCC ACAGACAGTC GTCCATGCCA 
GACAGCAGAT CCCCATGCTC CAGGCTGACA GTGAATGTGC CCATCAAAGC CAACTCTTTA 
GATGGCTTTG CTCAGAACTG CCCACAAGAT TTCCTAAGCG TGCAGCCGGT CAGTAGCGCG 
TCCTCATCCG GTCTCTGCAA ATCTGACTCT TGCTTGTATC GGAGAGGTGG GACTGACCAC 
ATCACCAACA TGTTAATTCA TGAAACGTGG GCTAGCTCCA TTGAGGCTCT CATGCGCAAG 
AACAAAATCA TTGTGGATGA TGCAGAGGAA GCTGACACTG AGCCTGTTTC TGGTGGCTCT 
CCCTCGCAAG CAGAGAAGTG TGCAAATAGA TTAGCTGCGA GCAGGATGTG CAGTGGGCCA 
ACTCTGCTTG TTCAGGAGTC TCTCGATTGC CCGAGGAAAG ACTCTGTTAC CGAATGTAAA 
CAGCCCCCAG TGTCATCTTT GAGCAAAACT GCTTCTCTTA CAAACCACAG CCCTTTAGAT 
TCTAAAAAAG AAACTTCCTC GTGCCAGGAC CCTGTACCAA TAAACCACAA AAGGCGATCA 
CTTTGCTCGA GGGAAGTGCC TTTGATTCAG ATTGAAACAG ATCAGAGAGA AGCCTGTGCT 
GGGGAACCTG AACCCTTCCT TTCCAAAAGC AGCCTCCTAG AGGAAGCAGA AGGGCATTCG 
AATGACAAAA ACATCCCAGA TGTGGTGAGA GGTGGAGACA CAGCCGTGAG CGCTTGTCAA 
ATCCATAGTG ACAGCCTTGA TACCAGAGAT GTACCAGAGG CTGAAGCCTC CACAGAAGCC 
AGAGCCCCCG ATGAGGCCCC CAACCCTCCA AGCAGCAGCG AGGAGAGCAC AGGCAGCTGG 
ACCCAGCTTG CCAATGAGGA AGACAACCCA GATGACACAA GTAGCTTTCT CCAGCTCAGT 
GAGCGATCCA TGAGCAATGG CAACAGTAGT GCCACTAGCA GTCTTGGCAT TATGGATCTG 
GACATTTATC AGGAAAGCAT GCCATCTTCT CCCATGATTA ATGAATTAGT AGAAGAAAAG 
AAGATTCTTA AAGGACAGTC AGAAAGCACA GAGGCACCTG CATCTGGACC GCCTACGGGA 
ACAGCCAGCC CCCAGAGGAG CCTGCTGGTG ATCAACTTTG ACCTGGAGCC AGAGTGTCCA 
GATGCCGAGC TCCGAGCCAC TCTGCAGTGG ATAGCTGCCT CTGAACTGGG GATTCCCACC 
ATCTACTTTA AGAAATCTCA GGAAAACAGA ATTGAAAAGT TTCTAGATGT CGTGCAGCTG 
GTTCATCGGA AGTCCTGGAA AGTGGGTGAT ATCTTCCATG CAGTTGTCCA GTACTGCAAA 
ATGCATGAGG AGCAGAAGGA TGGGAGACTG AGTCTCTTTG ACTGGCTCTT GGAACTGGGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( NM_001142644.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136116.1 Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09151
Clone ID Related Accession (Same CDS sequence)	NM_001142644.2 , NM_001142644.1
Accession Version	NM_001142644.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	A-kinase anchor protein SPHKAP isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC104956.1, AB051465.1 and AK095042.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC104954.1, SRR1803617.250221.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGGCA ACTCCCTGCT CTCGGTACCA AGCAACTTGG AGTCATCACG GATGTATGAC 
GTTTTGGAAC CGCAGCAGGG CAGAGGCTGT GGCAGCTCAG GAAGCGGCCC GGGGAACTCC 
ATCACAGCCT GTAAGAAGGT TCTTCGCAGC AATAGCCTGC TGGAGTCAAC AGACTACTGG 
TTGCAGAATC AGAGGATGCC CTGCCAAATT GGTTTTGTAG AAGACAAGTC TGAAAACTGT 
GCTTCTGTCT GCTTTGTGAA TCTTGATGTG AACAAGGATG AATGCAGCAC AGAGCACCTG 
CAACAGAAAC TGGTCAACGT TTCACCAGAT CTTCCAAAAC TTATCAGTTC CATGAATGTC 
CAACAACCAA AAGAAAATGA AATTGTTGTC CTAAGTGGGT TAGCCTCTGG AAATCTCCAG 
GCAGATTTTG AAGTTTCACA GTGCCCTTGG CTGCCAGATA TCTGCTTGGT CCAATGTGCA 
AGAGGGAACA GACCAAACAG TACCAACTGC ATCATCTTTG AAATCAACAA ATTTCTGATT 
GGTCTGGAAC TGGTGCAGGA GCGACAGCTC CACCTGGAAA CAAACATCTT GAAACTGGAG 
GATGACACGA ACTGTTCCTT ATCTTCAATC GAGGAAGACT TTCTCACCGC TTCTGAGCAC 
TTGGAGGAGG AAAGCGAGGT GGATGAATCT AGGAACGATT ATGAAAATAT AAATGTCTCA 
GCCAATGTTT TGGAAAGTAA ACAGCTAAAG GGAGCCACCC AGGTGGAATG GAATTGCAAC 
AAGGAAAAGT GGCTTTATGC TTTGGAAGAC AAATACATCA ACAAATATCC CACACCATTG 
ATTAAAACAG AACGATCTCC AGAAAACCTA ACAAAGAACA CAGCCTTGCA GAGTCTAGAT 
CCCTCAGCCA AGCCATCACA GTGGAAAAGA GAAGCTGTGG GGAATGGGAG ACAAGCCACA 
CATTATTATC ATTCAGAAGC TTTTAAAGGT CAAATGGAAA AATCACAGGC ACTGTATATT 
CCAAAAGATG CTTATTTCTC CATGATGGAT AAAGATGTAC CTTCTGCATG TGCTGTGGCA 
GAGCAGAGAA GCAACCTAAA CCCAGGAGAC CATGAAGACA CAAGAAACGC TCTCCCTCCT 
AGACAAGATG GAGAAGTCAC CACTGGCAAG TATGCTACAA ATTTAGCAGA ATCCGTGCTG 
CAGGATGCAT TTATTAGATT ATCTCAATCT CAGTCCACAT TACCCCAGGA ATCTGCAGTC 
AGTGTTTCTG TAGGAAGTTC TCTGCTTCCC AGTTGCTATT CCACAAAAGA TACAGTGGTT 
TCTCGGTCAT GGAATGAGCT CCCCAAAATC GTCGTTGTTC AGAGTCCAGA TGGCAGTGAT 
GCTGCCCCAC AGCCAGGCAT CTCCTCCTGG CCTGAGATGG AAGTCTCTGT TGAAACCTCA 
AGCATCCTCT CTGGAGAGAA CTCCAGCAGA CAACCCCAGA GTGCTCTAGA AGTGGCGTTA 
GCTTGTGCAG CCACTGTGAT TGGAACTATT TCCAGTCCAC AGGCCACAGA AAGACTCAAA 
ATGGAGCAAG TGGTCTCGAA CTTTCCCCCA GGGAGCAGTG GTGCACTGCA AACTCAAGCA 
CCCCAAGGAC TCAAGGAACC TTCCATCAAT GAGTACTCCT TTCCATCTGC TTTGTGTGGC 
ATGACTCAGG TGGCCAGTGC CGTGGCTGTC TGTGGTCTGG GTGAAAGAGA AGAGGTGACA 
TGCTCAGTGG CTCCAAGTGG TAGCCTCCCG CCTGCAGCTG AGGCTTCTGA AGCCATGCCC 
CCACTTTGTG GTTTAGCAAG CATGGAGCTT GGCAAGGAAG CCATTGCCAA GGGATTGCTC 
AAGGAGGCTG CTCTGGTTTT AACAAGGCCT AATACCTACA GCAGCATTGG AGACTTTCTG 
GACTCCATGA ACAGGAGAAT CATGGAAACT GCTTCAAAGT CTCAGACCCT GTGCTCAGAA 
AATGTCGTCA GGAATGAACT GGCACATACC CTGTCCAATG TTATCCTGAG GCATTCCATT 
GATGAAGTTC ACCACAAAAA TATGATAATC GACCCCAATG ACAACAGGCA TTCATCTGAA 
ATTCTGGACA CCTTAATGGA AAGTACAAAT CAACTGCTTT TAGATGTGAT ATGCTTCACG 
TTCAAGAAGA TGAGTCATAT TGTACGGCTT GGTGAATGTC CTGCTGTCCT TTCTAAGGAG 
ACCATCAGAA GGAGGGAGAC AGAACCAAGC TGCCAGCCAT CTGATCCGGG TGCTAGTCAA 
GCTTGGACAA AAGCCACTGA ATCCTCCAGC AGCTCTCCAC TTAGCAATTC ACACAACACG 
AGTCTTGTCA TCAACAATCT TGTGGATGGC ATGTATTCAA AACAAGACAA GGGTGGAGTG 
AGGCCAGGCC TCTTCAAGAA CCCCACGCTG CAGTCACAAT TATCACGTAG TCACAGAGTG 
CCCGATTCTT CAACTGCTAC AACATCCTCC AAGGAAATAT ATCTGAAAGG AATAGCAGGA 
GAGGATACAA AAAGCCCTCA TCACAGTGAG AATGAATGCA GAGCCTCTTC CGAAGGACAA 
AGGTCCCCAA CGGTCAGCCA GTCCAGAAGT GGTTCCCAGG AGGCTGAGGA GAGTATCCAC 
CCAAACACCC AAGAAAAGTA CAACTGTGCC ACATCTCGCA TCAACGAAGT TCAAGTCAAC 
CTGTCCTTGT TAGGGGATGA CCTGCTGCTT CCTGCTCAAT CCACGCTTCA AACAAAGCAT 
CCAGACATCT ACTGCATTAC AGACTTTGCG GAAGAATTAG CAGACACGGT CGTCTCCATG 
GCAACTGAAA TTGCAGCGAT TTGCCTTGAC AACTCCAGTG GAAAACAACC CTGGTTTTGT 
GCATGGAAAA GAGGGAGTGA GTTTCTGATG ACACCCAACG TACCCTGCCG ATCCTTGAAG 
AGGAAGAAAG AGAGCCAGGG GAGCGGGACC GCTGTGAGGA AACACAAGCC TCCCCGGCTC 
AGTGAGATCA AGAGGAAGAC GGACGAGCAC CCTGAGCTTA AAGAAAAGCT GATGAACAGG 
GTTGTGGATG AGTCCATGAA CCTTGAAGAT GTCCCAGATT CTGTCAATCT TTTTGCCAAT 
GAAGTGGCAG CCAAGATCAT GAACCTAACG GAGTTCTCTA TGGTGGACGG CATGTGGCAG 
GCGCAGGGCT ATCCCCGGAA TCGGTTACTG AGTGGCGACA GGTGGAGCCG GCTGAAGGCC 
TCCAGCTGCG AAAGCATTCC TGAGGAAGAC TCCGAGGCCA GGGCCTATGT CAACAGCCTG 
GGCTTAATGA GCACGCTGAG CCAGCCGGTC AGCAGGGCCA GCTCTGTCTC CAAGCAGTCG 
AGCTGTGAGA GCATCACCGA TGAGTTTTCC AGGTTCATGG TGAACCAGAT GGAAAATGAA 
GGGAGAGGAT TTGAGTTACT GCTGGATTAC TATGCTGGCA AGAACGCCAG CAGCATTCTG 
AACTCAGCCA TGCAACAGGC GTGCCGGAAA AGTGACCACC TCAGTGTGAG GCCTAGCTGT 
CCCTCTAAGC AGTCCAGCAC AGAGAGCATC ACTGAGGAGT TCTACAGGTA CATGCTGAGG 
GACATCGAAA GAGACAGCAG AGAAAGTGCC TCCTCCAGAC GGAGCAGCCA GGATTGGACA 
GCCGGCCTGC TGTCTCCTTC TCTGCGATCC CCAGTGTGCC ACAGACAGTC GTCCATGCCA 
GACAGCAGAT CCCCATGCTC CAGGCTGACA GTGAATGTGC CCATCAAAGC CAACTCTTTA 
GATGGCTTTG CTCAGAACTG CCCACAAGAT TTCCTAAGCG TGCAGCCGGT CAGTAGCGCG 
TCCTCATCCG GTCTCTGCAA ATCTGACTCT TGCTTGTATC GGAGAGGTGG GACTGACCAC 
ATCACCAACA TGTTAATTCA TGAAACGTGG GCTAGCTCCA TTGAGGCTCT CATGCGCAAG 
AACAAAATCA TTGTGGATGA TGCAGAGGAA GCTGACACTG AGCCTGTTTC TGGTGGCTCT 
CCCTCGCAAG CAGAGAAGTG TGCAAATAGA TTAGCTGCGA GCAGGATGTG CAGTGGGCCA 
ACTCTGCTTG TTCAGGAGTC TCTCGATTGC CCGAGGAAAG ACTCTGTTAC CGAATGTAAA 
CAGCCCCCAG TGTCATCTTT GAGCAAAACT GCTTCTCTTA CAAACCACAG CCCTTTAGAT 
TCTAAAAAAG AAACTTCCTC GTGCCAGGAC CCTGTACCAA TAAACCACAA AAGGCGATCA 
CTTTGCTCGA GGGAAGTGCC TTTGATTCAG ATTGAAACAG ATCAGAGAGA AGCCTGTGCT 
GGGGAACCTG AACCCTTCCT TTCCAAAAGC AGCCTCCTAG AGGAAGCAGA AGGGCATTCG 
AATGACAAAA ACATCCCAGA TGTGGTGAGA GGTGGAGACA CAGCCGTGAG CGCTTGTCAA 
ATCCATAGTG ACAGCCTTGA TACCAGAGAT GTACCAGAGG CTGAAGCCTC CACAGAAGCC 
AGAGCCCCCG ATGAGGCCCC CAACCCTCCA AGCAGCAGCG AGGAGAGCAC AGGCAGCTGG 
ACCCAGCTTG CCAATGAGGA AGACAACCCA GATGACACAA GTAGCTTTCT CCAGCTCAGT 
GAGCGATCCA TGAGCAATGG CAACAGTAGT GCCACTAGCA GTCTTGGCAT TATGGATCTG 
GACATTTATC AGGAAAGCAT GCCATCTTCT CCCATGATTA ATGAATTAGT AGAAGAAAAG 
AAGATTCTTA AAGGACAGTC AGAAAGCACA GAGGCACCTG CATCTGGACC GCCTACGGGA 
ACAGCCAGCC CCCAGAGGAG CCTGCTGGTG ATCAACTTTG ACCTGGAGCC AGAGTGTCCA 
GATGCCGAGC TCCGAGCCAC TCTGCAGTGG ATAGCTGCCT CTGAACTGGG GATTCCCACC 
ATCTACTTTA AGAAATCTCA GGAAAACAGA ATTGAAAAGT TTCTAGATGT CGTGCAGCTG 
GTTCATCGGA AGTCCTGGAA AGTGGGTGAT ATCTTCCATG CAGTTGTCCA GTACTGCAAA 
ATGCATGAGG AGCAGAAGGA TGGGAGACTG AGTCTCTTTG ACTGGCTCTT GGAACTGGGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136116.1
CDS	48..5150
Translation	MDGNSLLSVPSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQIGFVEDKSENCASVCFVNLDVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSIEEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPLIKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMDKDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAVSVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSILSGENSSRQPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCGMTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRPNTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSEILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSSSSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAGEDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLLPAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLKRKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLTEFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERDSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSLDGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEEADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLDSKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVRGGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLSERSMSNGNSSATSSLGIMDLDIYQESMPSSPMINELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCKMHEEQKDGRLSLFDWLLELG

Target ORF information:

RefSeq Version	NM_001142644.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142644.1

ORF Insert Sequence:

ATGGATGGCA ACTCCCTGCT CTCGGTACCA AGCAACTTGG AGTCATCACG GATGTATGAC 
GTTTTGGAAC CGCAGCAGGG CAGAGGCTGT GGCAGCTCAG GAAGCGGCCC GGGGAACTCC 
ATCACAGCCT GTAAGAAGGT TCTTCGCAGC AATAGCCTGC TGGAGTCAAC AGACTACTGG 
TTGCAGAATC AGAGGATGCC CTGCCAAATT GGTTTTGTAG AAGACAAGTC TGAAAACTGT 
GCTTCTGTCT GCTTTGTGAA TCTTGATGTG AACAAGGATG AATGCAGCAC AGAGCACCTG 
CAACAGAAAC TGGTCAACGT TTCACCAGAT CTTCCAAAAC TTATCAGTTC CATGAATGTC 
CAACAACCAA AAGAAAATGA AATTGTTGTC CTAAGTGGGT TAGCCTCTGG AAATCTCCAG 
GCAGATTTTG AAGTTTCACA GTGCCCTTGG CTGCCAGATA TCTGCTTGGT CCAATGTGCA 
AGAGGGAACA GACCAAACAG TACCAACTGC ATCATCTTTG AAATCAACAA ATTTCTGATT 
GGTCTGGAAC TGGTGCAGGA GCGACAGCTC CACCTGGAAA CAAACATCTT GAAACTGGAG 
GATGACACGA ACTGTTCCTT ATCTTCAATC GAGGAAGACT TTCTCACCGC TTCTGAGCAC 
TTGGAGGAGG AAAGCGAGGT GGATGAATCT AGGAACGATT ATGAAAATAT AAATGTCTCA 
GCCAATGTTT TGGAAAGTAA ACAGCTAAAG GGAGCCACCC AGGTGGAATG GAATTGCAAC 
AAGGAAAAGT GGCTTTATGC TTTGGAAGAC AAATACATCA ACAAATATCC CACACCATTG 
ATTAAAACAG AACGATCTCC AGAAAACCTA ACAAAGAACA CAGCCTTGCA GAGTCTAGAT 
CCCTCAGCCA AGCCATCACA GTGGAAAAGA GAAGCTGTGG GGAATGGGAG ACAAGCCACA 
CATTATTATC ATTCAGAAGC TTTTAAAGGT CAAATGGAAA AATCACAGGC ACTGTATATT 
CCAAAAGATG CTTATTTCTC CATGATGGAT AAAGATGTAC CTTCTGCATG TGCTGTGGCA 
GAGCAGAGAA GCAACCTAAA CCCAGGAGAC CATGAAGACA CAAGAAACGC TCTCCCTCCT 
AGACAAGATG GAGAAGTCAC CACTGGCAAG TATGCTACAA ATTTAGCAGA ATCCGTGCTG 
CAGGATGCAT TTATTAGATT ATCTCAATCT CAGTCCACAT TACCCCAGGA ATCTGCAGTC 
AGTGTTTCTG TAGGAAGTTC TCTGCTTCCC AGTTGCTATT CCACAAAAGA TACAGTGGTT 
TCTCGGTCAT GGAATGAGCT CCCCAAAATC GTCGTTGTTC AGAGTCCAGA TGGCAGTGAT 
GCTGCCCCAC AGCCAGGCAT CTCCTCCTGG CCTGAGATGG AAGTCTCTGT TGAAACCTCA 
AGCATCCTCT CTGGAGAGAA CTCCAGCAGA CAACCCCAGA GTGCTCTAGA AGTGGCGTTA 
GCTTGTGCAG CCACTGTGAT TGGAACTATT TCCAGTCCAC AGGCCACAGA AAGACTCAAA 
ATGGAGCAAG TGGTCTCGAA CTTTCCCCCA GGGAGCAGTG GTGCACTGCA AACTCAAGCA 
CCCCAAGGAC TCAAGGAACC TTCCATCAAT GAGTACTCCT TTCCATCTGC TTTGTGTGGC 
ATGACTCAGG TGGCCAGTGC CGTGGCTGTC TGTGGTCTGG GTGAAAGAGA AGAGGTGACA 
TGCTCAGTGG CTCCAAGTGG TAGCCTCCCG CCTGCAGCTG AGGCTTCTGA AGCCATGCCC 
CCACTTTGTG GTTTAGCAAG CATGGAGCTT GGCAAGGAAG CCATTGCCAA GGGATTGCTC 
AAGGAGGCTG CTCTGGTTTT AACAAGGCCT AATACCTACA GCAGCATTGG AGACTTTCTG 
GACTCCATGA ACAGGAGAAT CATGGAAACT GCTTCAAAGT CTCAGACCCT GTGCTCAGAA 
AATGTCGTCA GGAATGAACT GGCACATACC CTGTCCAATG TTATCCTGAG GCATTCCATT 
GATGAAGTTC ACCACAAAAA TATGATAATC GACCCCAATG ACAACAGGCA TTCATCTGAA 
ATTCTGGACA CCTTAATGGA AAGTACAAAT CAACTGCTTT TAGATGTGAT ATGCTTCACG 
TTCAAGAAGA TGAGTCATAT TGTACGGCTT GGTGAATGTC CTGCTGTCCT TTCTAAGGAG 
ACCATCAGAA GGAGGGAGAC AGAACCAAGC TGCCAGCCAT CTGATCCGGG TGCTAGTCAA 
GCTTGGACAA AAGCCACTGA ATCCTCCAGC AGCTCTCCAC TTAGCAATTC ACACAACACG 
AGTCTTGTCA TCAACAATCT TGTGGATGGC ATGTATTCAA AACAAGACAA GGGTGGAGTG 
AGGCCAGGCC TCTTCAAGAA CCCCACGCTG CAGTCACAAT TATCACGTAG TCACAGAGTG 
CCCGATTCTT CAACTGCTAC AACATCCTCC AAGGAAATAT ATCTGAAAGG AATAGCAGGA 
GAGGATACAA AAAGCCCTCA TCACAGTGAG AATGAATGCA GAGCCTCTTC CGAAGGACAA 
AGGTCCCCAA CGGTCAGCCA GTCCAGAAGT GGTTCCCAGG AGGCTGAGGA GAGTATCCAC 
CCAAACACCC AAGAAAAGTA CAACTGTGCC ACATCTCGCA TCAACGAAGT TCAAGTCAAC 
CTGTCCTTGT TAGGGGATGA CCTGCTGCTT CCTGCTCAAT CCACGCTTCA AACAAAGCAT 
CCAGACATCT ACTGCATTAC AGACTTTGCG GAAGAATTAG CAGACACGGT CGTCTCCATG 
GCAACTGAAA TTGCAGCGAT TTGCCTTGAC AACTCCAGTG GAAAACAACC CTGGTTTTGT 
GCATGGAAAA GAGGGAGTGA GTTTCTGATG ACACCCAACG TACCCTGCCG ATCCTTGAAG 
AGGAAGAAAG AGAGCCAGGG GAGCGGGACC GCTGTGAGGA AACACAAGCC TCCCCGGCTC 
AGTGAGATCA AGAGGAAGAC GGACGAGCAC CCTGAGCTTA AAGAAAAGCT GATGAACAGG 
GTTGTGGATG AGTCCATGAA CCTTGAAGAT GTCCCAGATT CTGTCAATCT TTTTGCCAAT 
GAAGTGGCAG CCAAGATCAT GAACCTAACG GAGTTCTCTA TGGTGGACGG CATGTGGCAG 
GCGCAGGGCT ATCCCCGGAA TCGGTTACTG AGTGGCGACA GGTGGAGCCG GCTGAAGGCC 
TCCAGCTGCG AAAGCATTCC TGAGGAAGAC TCCGAGGCCA GGGCCTATGT CAACAGCCTG 
GGCTTAATGA GCACGCTGAG CCAGCCGGTC AGCAGGGCCA GCTCTGTCTC CAAGCAGTCG 
AGCTGTGAGA GCATCACCGA TGAGTTTTCC AGGTTCATGG TGAACCAGAT GGAAAATGAA 
GGGAGAGGAT TTGAGTTACT GCTGGATTAC TATGCTGGCA AGAACGCCAG CAGCATTCTG 
AACTCAGCCA TGCAACAGGC GTGCCGGAAA AGTGACCACC TCAGTGTGAG GCCTAGCTGT 
CCCTCTAAGC AGTCCAGCAC AGAGAGCATC ACTGAGGAGT TCTACAGGTA CATGCTGAGG 
GACATCGAAA GAGACAGCAG AGAAAGTGCC TCCTCCAGAC GGAGCAGCCA GGATTGGACA 
GCCGGCCTGC TGTCTCCTTC TCTGCGATCC CCAGTGTGCC ACAGACAGTC GTCCATGCCA 
GACAGCAGAT CCCCATGCTC CAGGCTGACA GTGAATGTGC CCATCAAAGC CAACTCTTTA 
GATGGCTTTG CTCAGAACTG CCCACAAGAT TTCCTAAGCG TGCAGCCGGT CAGTAGCGCG 
TCCTCATCCG GTCTCTGCAA ATCTGACTCT TGCTTGTATC GGAGAGGTGG GACTGACCAC 
ATCACCAACA TGTTAATTCA TGAAACGTGG GCTAGCTCCA TTGAGGCTCT CATGCGCAAG 
AACAAAATCA TTGTGGATGA TGCAGAGGAA GCTGACACTG AGCCTGTTTC TGGTGGCTCT 
CCCTCGCAAG CAGAGAAGTG TGCAAATAGA TTAGCTGCGA GCAGGATGTG CAGTGGGCCA 
ACTCTGCTTG TTCAGGAGTC TCTCGATTGC CCGAGGAAAG ACTCTGTTAC CGAATGTAAA 
CAGCCCCCAG TGTCATCTTT GAGCAAAACT GCTTCTCTTA CAAACCACAG CCCTTTAGAT 
TCTAAAAAAG AAACTTCCTC GTGCCAGGAC CCTGTACCAA TAAACCACAA AAGGCGATCA 
CTTTGCTCGA GGGAAGTGCC TTTGATTCAG ATTGAAACAG ATCAGAGAGA AGCCTGTGCT 
GGGGAACCTG AACCCTTCCT TTCCAAAAGC AGCCTCCTAG AGGAAGCAGA AGGGCATTCG 
AATGACAAAA ACATCCCAGA TGTGGTGAGA GGTGGAGACA CAGCCGTGAG CGCTTGTCAA 
ATCCATAGTG ACAGCCTTGA TACCAGAGAT GTACCAGAGG CTGAAGCCTC CACAGAAGCC 
AGAGCCCCCG ATGAGGCCCC CAACCCTCCA AGCAGCAGCG AGGAGAGCAC AGGCAGCTGG 
ACCCAGCTTG CCAATGAGGA AGACAACCCA GATGACACAA GTAGCTTTCT CCAGCTCAGT 
GAGCGATCCA TGAGCAATGG CAACAGTAGT GCCACTAGCA GTCTTGGCAT TATGGATCTG 
GACATTTATC AGGAAAGCAT GCCATCTTCT CCCATGATTA ATGAATTAGT AGAAGAAAAG 
AAGATTCTTA AAGGACAGTC AGAAAGCACA GAGGCACCTG CATCTGGACC GCCTACGGGA 
ACAGCCAGCC CCCAGAGGAG CCTGCTGGTG ATCAACTTTG ACCTGGAGCC AGAGTGTCCA 
GATGCCGAGC TCCGAGCCAC TCTGCAGTGG ATAGCTGCCT CTGAACTGGG GATTCCCACC 
ATCTACTTTA AGAAATCTCA GGAAAACAGA ATTGAAAAGT TTCTAGATGT CGTGCAGCTG 
GTTCATCGGA AGTCCTGGAA AGTGGGTGAT ATCTTCCATG CAGTTGTCCA GTACTGCAAA 
ATGCATGAGG AGCAGAAGGA TGGGAGACTG AGTCTCTTTG ACTGGCTCTT GGAACTGGGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_005246870.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246927.1 Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu36652
Clone ID Related Accession (Same CDS sequence)	XM_005246870.4
Accession Version	XM_005246870.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	A-kinase anchor protein SPHKAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005246870.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGACG TTTTGGAACC GCAGCAGGGC AGAGGCTGTG GCAGCTCAGG AAGCGGCCCG 
GGGAACTCCA TCACAGCCTG TAAGAAGGTT CTTCGCAGCA ATAGCCTGCT GGAGTCAACA 
GACTACTGGT TGCAGAATCA GAGGATGCCC TGCCAAATTG GTTTTGTAGA AGACAAGTCT 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAT CTTGATGTGA ACAAGGATGA ATGCAGCACA 
GAGCACCTGC AACAGAAACT GGTCAACGTT TCACCAGATC TTCCAAAACT TATCAGTTCC 
ATGAATGTCC AACAACCAAA AGAAAATGAA ATTGTTGTCC TAAGTGGGTT AGCCTCTGGA 
AATCTCCAGG CAGATTTTGA AGTTTCACAG TGCCCTTGGC TGCCAGATAT CTGCTTGGTC 
CAATGTGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA AATCAACAAA 
TTTCTGATTG GTCTGGAACT GGTGCAGGAG CGACAGCTCC ACCTGGAAAC AAACATCTTG 
AAACTGGAGG ATGACACGAA CTGTTCCTTA TCTTCAATCG AGGAAGACTT TCTCACCGCT 
TCTGAGCACT TGGAGGAGGA AAGCGAGGTG GATGAATCTA GGAACGATTA TGAAAATATA 
AATGTCTCAG CCAATGTTTT GGAAAGTAAA CAGCTAAAGG GAGCCACCCA GGTGGAATGG 
AATTGCAACA AGGAAAAGTG GCTTTATGCT TTGGAAGACA AATACATCAA CAAATATCCC 
ACACCATTGA TTAAAACAGA ACGATCTCCA GAAAACCTAA CAAAGAACAC AGCCTTGCAG 
AGTCTAGATC CCTCAGCCAA GCCATCACAG TGGAAAAGAG AAGCTGTGGG GAATGGGAGA 
CAAGCCACAC ATTATTATCA TTCAGAAGCT TTTAAAGGTC AAATGGAAAA ATCACAGGCA 
CTGTATATTC CAAAAGATGC TTATTTCTCC ATGATGGATA AAGATGTACC TTCTGCATGT 
GCTGTGGCAG AGCAGAGAAG CAACCTAAAC CCAGGAGACC ATGAAGACAC AAGAAACGCT 
CTCCCTCCTA GACAAGATGG AGAAGTCACC ACTGGCAAGT ATGCTACAAA TTTAGCAGAA 
TCCGTGCTGC AGGATGCATT TATTAGATTA TCTCAATCTC AGTCCACATT ACCCCAGGAA 
TCTGCAGTCA GTGTTTCTGT AGGAAGTTCT CTGCTTCCCA GTTGCTATTC CACAAAAGAT 
ACAGTGGTTT CTCGGTCATG GAATGAGCTC CCCAAAATCG TCGTTGTTCA GAGTCCAGAT 
GGCAGTGATG CTGCCCCACA GCCAGGCATC TCCTCCTGGC CTGAGATGGA AGTCTCTGTT 
GAAACCTCAA GCATCCTCTC TGGAGAGAAC TCCAGCAGAC AACCCCAGAG TGCTCTAGAA 
GTGGCGTTAG CTTGTGCAGC CACTGTGATT GGAACTATTT CCAGTCCACA GGCCACAGAA 
AGACTCAAAA TGGAGCAAGT GGTCTCGAAC TTTCCCCCAG GGAGCAGTGG TGCACTGCAA 
ACTCAAGCAC CCCAAGGACT CAAGGAACCT TCCATCAATG AGTACTCCTT TCCATCTGCT 
TTGTGTGGCA TGACTCAGGT GGCCAGTGCC GTGGCTGTCT GTGGTCTGGG TGAAAGAGAA 
GAGGTGACAT GCTCAGTGGC TCCAAGTGGT AGCCTCCCGC CTGCAGCTGA GGCTTCTGAA 
GCCATGCCCC CACTTTGTGG TTTAGCAAGC ATGGAGCTTG GCAAGGAAGC CATTGCCAAG 
GGATTGCTCA AGGAGGCTGC TCTGGTTTTA ACAAGGCCTA ATACCTACAG CAGCATTGGA 
GACTTTCTGG ACTCCATGAA CAGGAGAATC ATGGAAACTG CTTCAAAGTC TCAGACCCTG 
TGCTCAGAAA ATGTCGTCAG GAATGAACTG GCACATACCC TGTCCAATGT TATCCTGAGG 
CATTCCATTG ATGAAGTTCA CCACAAAAAT ATGATAATCG ACCCCAATGA CAACAGGCAT 
TCATCTGAAA TTCTGGACAC CTTAATGGAA AGTACAAATC AACTGCTTTT AGATGTGATA 
TGCTTCACGT TCAAGAAGAT GAGTCATATT GTACGGCTTG GTGAATGTCC TGCTGTCCTT 
TCTAAGGAGA CCATCAGAAG GAGGGAGACA GAACCAAGCT GCCAGCCATC TGATCCGGGT 
GCTAGTCAAG CTTGGACAAA AGCCACTGAA TCCTCCAGCA GCTCTCCACT TAGCAATTCA 
CACAACACGA GTCTTGTCAT CAACAATCTT GTGGATGGCA TGTATTCAAA ACAAGACAAG 
GGTGGAGTGA GGCCAGGCCT CTTCAAGAAC CCCACGCTGC AGTCACAATT ATCACGTAGT 
CACAGAGTGC CCGATTCTTC AACTGCTACA ACATCCTCCA AGGAAATATA TCTGAAAGGA 
ATAGCAGGAG AGGATACAAA AAGCCCTCAT CACAGTGAGA ATGAATGCAG AGCCTCTTCC 
GAAGGACAAA GGTCCCCAAC GGTCAGCCAG TCCAGAAGTG GTTCCCAGGA GGCTGAGGAG 
AGTATCCACC CAAACACCCA AGAAAAGTAC AACTGTGCCA CATCTCGCAT CAACGAAGTT 
CAAGTCAACC TGTCCTTGTT AGGGGATGAC CTGCTGCTTC CTGCTCAATC CACGCTTCAA 
ACAAAGCATC CAGACATCTA CTGCATTACA GACTTTGCGG AAGAATTAGC AGACACGGTC 
GTCTCCATGG CAACTGAAAT TGCAGCGATT TGCCTTGACA ACTCCAGTGG AAAACAACCC 
TGGTTTTGTG CATGGAAAAG AGGGAGTGAG TTTCTGATGA CACCCAACGT ACCCTGCCGA 
TCCTTGAAGA GGAAGAAAGA GAGCCAGGGG AGCGGGACCG CTGTGAGGAA ACACAAGCCT 
CCCCGGCTCA GTGAGATCAA GAGGAAGACG GACGAGCACC CTGAGCTTAA AGAAAAGCTG 
ATGAACAGGG TTGTGGATGA GTCCATGAAC CTTGAAGATG TCCCAGATTC TGTCAATCTT 
TTTGCCAATG AAGTGGCAGC CAAGATCATG AACCTAACGG AGTTCTCTAT GGTGGACGGC 
ATGTGGCAGG CGCAGGGCTA TCCCCGGAAT CGGTTACTGA GTGGCGACAG GTGGAGCCGG 
CTGAAGGCCT CCAGCTGCGA AAGCATTCCT GAGGAAGACT CCGAGGCCAG GGCCTATGTC 
AACAGCCTGG GCTTAATGAG CACGCTGAGC CAGCCGGTCA GCAGGGCCAG CTCTGTCTCC 
AAGCAGTCGA GCTGTGAGAG CATCACCGAT GAGTTTTCCA GGTTCATGGT GAACCAGATG 
GAAAATGAAG GGAGAGGATT TGAGTTACTG CTGGATTACT ATGCTGGCAA GAACGCCAGC 
AGCATTCTGA ACTCAGCCAT GCAACAGGCG TGCCGGAAAA GTGACCACCT CAGTGTGAGG 
CCTAGCTGTC CCTCTAAGCA GTCCAGCACA GAGAGCATCA CTGAGGAGTT CTACAGGTAC 
ATGCTGAGGG ACATCGAAAG AGACAGCAGA GAAAGTGCCT CCTCCAGACG GAGCAGCCAG 
GATTGGACAG CCGGCCTGCT GTCTCCTTCT CTGCGATCCC CAGTGTGCCA CAGACAGTCG 
TCCATGCCAG ACAGCAGATC CCCATGCTCC AGGCTGACAG TGAATGTGCC CATCAAAGCC 
AACTCTTTAG ATGGCTTTGC TCAGAACTGC CCACAAGATT TCCTAAGCGT GCAGCCGGTC 
AGTAGCGCGT CCTCATCCGG TCTCTGCAAA TCTGACTCTT GCTTGTATCG GAGAGGTGGG 
ACTGACCACA TCACCAACAT GTTAATTCAT GAAACGTGGG CTAGCTCCAT TGAGGCTCTC 
ATGCGCAAGA ACAAAATCAT TGTGGATGAT GCAGAGGAAG CTGACACTGA GCCTGTTTCT 
GGTGGCTCTC CCTCGCAAGC AGAGAAGTGT GCAAATAGAT TAGCTGCGAG CAGGATGTGC 
AGTGGGCCAA CTCTGCTTGT TCAGGAGTCT CTCGATTGCC CGAGGAAAGA CTCTGTTACC 
GAATGTAAAC AGCCCCCAGT GTCATCTTTG AGCAAAACTG CTTCTCTTAC AAACCACAGC 
CCTTTAGATT CTAAAAAAGA AACTTCCTCG TGCCAGGACC CTGTACCAAT AAACCACAAA 
AGGCGATCAC TTTGCTCGAG GGAAGTGCCT TTGATTCAGA TTGAAACAGA TCAGAGAGAA 
GCCTGTGCTG GGGAACCTGA ACCCTTCCTT TCCAAAAGCA GCCTCCTAGA GGAAGCAGAA 
GGGCATTCGA ATGACAAAAA CATCCCAGAT GTGGTGAGAG GTGGAGACAC AGCCGTGAGC 
GCTTGTCAAA TCCATAGTGA CAGCCTTGAT ACCAGAGATG TACCAGAGGC TGAAGCCTCC 
ACAGAAGCCA GAGCCCCCGA TGAGGCCCCC AACCCTCCAA GCAGCAGCGA GGAGAGCACA 
GGCAGCTGGA CCCAGCTTGC CAATGAGGAA GACAACCCAG ATGACACAAG TAGCTTTCTC 
CAGCTCAGTG AGCGATCCAT GAGCAATGGC AACAGTAGTG CCACTAGCAG TCTTGGCATT 
ATGGATCTGG ACATTTATCA GGAAAGCATG CCATCTTCTC CCATGATTAA TGAATTAGTA 
GAAGAAAAGA AGATTCTTAA AGGACAGTCA GAAAGCACAG AGGCACCTGC ATCTGGACCG 
CCTACGGGAA CAGCCAGCCC CCAGAGGAGC CTGCTGGTGA TCAACTTTGA CCTGGAGCCA 
GAGTGTCCAG ATGCCGAGCT CCGAGCCACT CTGCAGTGGA TAGCTGCCTC TGAACTGGGG 
ATTCCCACCA TCTACTTTAA GAAATCTCAG GAAAACAGAA TTGAAAAGTT TCTAGATGTC 
GTGCAGCTGG TTCATCGGAA GTCCTGGAAA GTGGGTGATA TCTTCCATGC AGTTGTCCAG 
TACTGCAAAA TGCATGAGGA GCAGAAGGAT GGGAGACTGA GTCTCTTTGA CTGGCTCTTG 
GAACTGGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246927.1
CDS	571..5622
Translation	MYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQIGFVEDKSENCASVCFVNLDVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSIEEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPLIKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMDKDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAVSVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSILSGENSSRQPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCGMTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRPNTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSEILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSSSSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAGEDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLLPAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLKRKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLTEFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERDSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSLDGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEEADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLDSKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVRGGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLSERSMSNGNSSATSSLGIMDLDIYQESMPSSPMINELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCKMHEEQKDGRLSLFDWLLELG

Target ORF information:

RefSeq Version	XM_005246870.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246870.4

ORF Insert Sequence:

ATGTATGACG TTTTGGAACC GCAGCAGGGC AGAGGCTGTG GCAGCTCAGG AAGCGGCCCG 
GGGAACTCCA TCACAGCCTG TAAGAAGGTT CTTCGCAGCA ATAGCCTGCT GGAGTCAACA 
GACTACTGGT TGCAGAATCA GAGGATGCCC TGCCAAATTG GTTTTGTAGA AGACAAGTCT 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAT CTTGATGTGA ACAAGGATGA ATGCAGCACA 
GAGCACCTGC AACAGAAACT GGTCAACGTT TCACCAGATC TTCCAAAACT TATCAGTTCC 
ATGAATGTCC AACAACCAAA AGAAAATGAA ATTGTTGTCC TAAGTGGGTT AGCCTCTGGA 
AATCTCCAGG CAGATTTTGA AGTTTCACAG TGCCCTTGGC TGCCAGATAT CTGCTTGGTC 
CAATGTGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA AATCAACAAA 
TTTCTGATTG GTCTGGAACT GGTGCAGGAG CGACAGCTCC ACCTGGAAAC AAACATCTTG 
AAACTGGAGG ATGACACGAA CTGTTCCTTA TCTTCAATCG AGGAAGACTT TCTCACCGCT 
TCTGAGCACT TGGAGGAGGA AAGCGAGGTG GATGAATCTA GGAACGATTA TGAAAATATA 
AATGTCTCAG CCAATGTTTT GGAAAGTAAA CAGCTAAAGG GAGCCACCCA GGTGGAATGG 
AATTGCAACA AGGAAAAGTG GCTTTATGCT TTGGAAGACA AATACATCAA CAAATATCCC 
ACACCATTGA TTAAAACAGA ACGATCTCCA GAAAACCTAA CAAAGAACAC AGCCTTGCAG 
AGTCTAGATC CCTCAGCCAA GCCATCACAG TGGAAAAGAG AAGCTGTGGG GAATGGGAGA 
CAAGCCACAC ATTATTATCA TTCAGAAGCT TTTAAAGGTC AAATGGAAAA ATCACAGGCA 
CTGTATATTC CAAAAGATGC TTATTTCTCC ATGATGGATA AAGATGTACC TTCTGCATGT 
GCTGTGGCAG AGCAGAGAAG CAACCTAAAC CCAGGAGACC ATGAAGACAC AAGAAACGCT 
CTCCCTCCTA GACAAGATGG AGAAGTCACC ACTGGCAAGT ATGCTACAAA TTTAGCAGAA 
TCCGTGCTGC AGGATGCATT TATTAGATTA TCTCAATCTC AGTCCACATT ACCCCAGGAA 
TCTGCAGTCA GTGTTTCTGT AGGAAGTTCT CTGCTTCCCA GTTGCTATTC CACAAAAGAT 
ACAGTGGTTT CTCGGTCATG GAATGAGCTC CCCAAAATCG TCGTTGTTCA GAGTCCAGAT 
GGCAGTGATG CTGCCCCACA GCCAGGCATC TCCTCCTGGC CTGAGATGGA AGTCTCTGTT 
GAAACCTCAA GCATCCTCTC TGGAGAGAAC TCCAGCAGAC AACCCCAGAG TGCTCTAGAA 
GTGGCGTTAG CTTGTGCAGC CACTGTGATT GGAACTATTT CCAGTCCACA GGCCACAGAA 
AGACTCAAAA TGGAGCAAGT GGTCTCGAAC TTTCCCCCAG GGAGCAGTGG TGCACTGCAA 
ACTCAAGCAC CCCAAGGACT CAAGGAACCT TCCATCAATG AGTACTCCTT TCCATCTGCT 
TTGTGTGGCA TGACTCAGGT GGCCAGTGCC GTGGCTGTCT GTGGTCTGGG TGAAAGAGAA 
GAGGTGACAT GCTCAGTGGC TCCAAGTGGT AGCCTCCCGC CTGCAGCTGA GGCTTCTGAA 
GCCATGCCCC CACTTTGTGG TTTAGCAAGC ATGGAGCTTG GCAAGGAAGC CATTGCCAAG 
GGATTGCTCA AGGAGGCTGC TCTGGTTTTA ACAAGGCCTA ATACCTACAG CAGCATTGGA 
GACTTTCTGG ACTCCATGAA CAGGAGAATC ATGGAAACTG CTTCAAAGTC TCAGACCCTG 
TGCTCAGAAA ATGTCGTCAG GAATGAACTG GCACATACCC TGTCCAATGT TATCCTGAGG 
CATTCCATTG ATGAAGTTCA CCACAAAAAT ATGATAATCG ACCCCAATGA CAACAGGCAT 
TCATCTGAAA TTCTGGACAC CTTAATGGAA AGTACAAATC AACTGCTTTT AGATGTGATA 
TGCTTCACGT TCAAGAAGAT GAGTCATATT GTACGGCTTG GTGAATGTCC TGCTGTCCTT 
TCTAAGGAGA CCATCAGAAG GAGGGAGACA GAACCAAGCT GCCAGCCATC TGATCCGGGT 
GCTAGTCAAG CTTGGACAAA AGCCACTGAA TCCTCCAGCA GCTCTCCACT TAGCAATTCA 
CACAACACGA GTCTTGTCAT CAACAATCTT GTGGATGGCA TGTATTCAAA ACAAGACAAG 
GGTGGAGTGA GGCCAGGCCT CTTCAAGAAC CCCACGCTGC AGTCACAATT ATCACGTAGT 
CACAGAGTGC CCGATTCTTC AACTGCTACA ACATCCTCCA AGGAAATATA TCTGAAAGGA 
ATAGCAGGAG AGGATACAAA AAGCCCTCAT CACAGTGAGA ATGAATGCAG AGCCTCTTCC 
GAAGGACAAA GGTCCCCAAC GGTCAGCCAG TCCAGAAGTG GTTCCCAGGA GGCTGAGGAG 
AGTATCCACC CAAACACCCA AGAAAAGTAC AACTGTGCCA CATCTCGCAT CAACGAAGTT 
CAAGTCAACC TGTCCTTGTT AGGGGATGAC CTGCTGCTTC CTGCTCAATC CACGCTTCAA 
ACAAAGCATC CAGACATCTA CTGCATTACA GACTTTGCGG AAGAATTAGC AGACACGGTC 
GTCTCCATGG CAACTGAAAT TGCAGCGATT TGCCTTGACA ACTCCAGTGG AAAACAACCC 
TGGTTTTGTG CATGGAAAAG AGGGAGTGAG TTTCTGATGA CACCCAACGT ACCCTGCCGA 
TCCTTGAAGA GGAAGAAAGA GAGCCAGGGG AGCGGGACCG CTGTGAGGAA ACACAAGCCT 
CCCCGGCTCA GTGAGATCAA GAGGAAGACG GACGAGCACC CTGAGCTTAA AGAAAAGCTG 
ATGAACAGGG TTGTGGATGA GTCCATGAAC CTTGAAGATG TCCCAGATTC TGTCAATCTT 
TTTGCCAATG AAGTGGCAGC CAAGATCATG AACCTAACGG AGTTCTCTAT GGTGGACGGC 
ATGTGGCAGG CGCAGGGCTA TCCCCGGAAT CGGTTACTGA GTGGCGACAG GTGGAGCCGG 
CTGAAGGCCT CCAGCTGCGA AAGCATTCCT GAGGAAGACT CCGAGGCCAG GGCCTATGTC 
AACAGCCTGG GCTTAATGAG CACGCTGAGC CAGCCGGTCA GCAGGGCCAG CTCTGTCTCC 
AAGCAGTCGA GCTGTGAGAG CATCACCGAT GAGTTTTCCA GGTTCATGGT GAACCAGATG 
GAAAATGAAG GGAGAGGATT TGAGTTACTG CTGGATTACT ATGCTGGCAA GAACGCCAGC 
AGCATTCTGA ACTCAGCCAT GCAACAGGCG TGCCGGAAAA GTGACCACCT CAGTGTGAGG 
CCTAGCTGTC CCTCTAAGCA GTCCAGCACA GAGAGCATCA CTGAGGAGTT CTACAGGTAC 
ATGCTGAGGG ACATCGAAAG AGACAGCAGA GAAAGTGCCT CCTCCAGACG GAGCAGCCAG 
GATTGGACAG CCGGCCTGCT GTCTCCTTCT CTGCGATCCC CAGTGTGCCA CAGACAGTCG 
TCCATGCCAG ACAGCAGATC CCCATGCTCC AGGCTGACAG TGAATGTGCC CATCAAAGCC 
AACTCTTTAG ATGGCTTTGC TCAGAACTGC CCACAAGATT TCCTAAGCGT GCAGCCGGTC 
AGTAGCGCGT CCTCATCCGG TCTCTGCAAA TCTGACTCTT GCTTGTATCG GAGAGGTGGG 
ACTGACCACA TCACCAACAT GTTAATTCAT GAAACGTGGG CTAGCTCCAT TGAGGCTCTC 
ATGCGCAAGA ACAAAATCAT TGTGGATGAT GCAGAGGAAG CTGACACTGA GCCTGTTTCT 
GGTGGCTCTC CCTCGCAAGC AGAGAAGTGT GCAAATAGAT TAGCTGCGAG CAGGATGTGC 
AGTGGGCCAA CTCTGCTTGT TCAGGAGTCT CTCGATTGCC CGAGGAAAGA CTCTGTTACC 
GAATGTAAAC AGCCCCCAGT GTCATCTTTG AGCAAAACTG CTTCTCTTAC AAACCACAGC 
CCTTTAGATT CTAAAAAAGA AACTTCCTCG TGCCAGGACC CTGTACCAAT AAACCACAAA 
AGGCGATCAC TTTGCTCGAG GGAAGTGCCT TTGATTCAGA TTGAAACAGA TCAGAGAGAA 
GCCTGTGCTG GGGAACCTGA ACCCTTCCTT TCCAAAAGCA GCCTCCTAGA GGAAGCAGAA 
GGGCATTCGA ATGACAAAAA CATCCCAGAT GTGGTGAGAG GTGGAGACAC AGCCGTGAGC 
GCTTGTCAAA TCCATAGTGA CAGCCTTGAT ACCAGAGATG TACCAGAGGC TGAAGCCTCC 
ACAGAAGCCA GAGCCCCCGA TGAGGCCCCC AACCCTCCAA GCAGCAGCGA GGAGAGCACA 
GGCAGCTGGA CCCAGCTTGC CAATGAGGAA GACAACCCAG ATGACACAAG TAGCTTTCTC 
CAGCTCAGTG AGCGATCCAT GAGCAATGGC AACAGTAGTG CCACTAGCAG TCTTGGCATT 
ATGGATCTGG ACATTTATCA GGAAAGCATG CCATCTTCTC CCATGATTAA TGAATTAGTA 
GAAGAAAAGA AGATTCTTAA AGGACAGTCA GAAAGCACAG AGGCACCTGC ATCTGGACCG 
CCTACGGGAA CAGCCAGCCC CCAGAGGAGC CTGCTGGTGA TCAACTTTGA CCTGGAGCCA 
GAGTGTCCAG ATGCCGAGCT CCGAGCCACT CTGCAGTGGA TAGCTGCCTC TGAACTGGGG 
ATTCCCACCA TCTACTTTAA GAAATCTCAG GAAAACAGAA TTGAAAAGTT TCTAGATGTC 
GTGCAGCTGG TTCATCGGAA GTCCTGGAAA GTGGGTGATA TCTTCCATGC AGTTGTCCAG 
TACTGCAAAA TGCATGAGGA GCAGAAGGAT GGGAGACTGA GTCTCTTTGA CTGGCTCTTG 
GAACTGGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( NM_030623.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_085126.2 Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 2, mRNA.

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CloneID	OHu09199
Clone ID Related Accession (Same CDS sequence)	NM_030623.3
Accession Version	NM_030623.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	A-kinase anchor protein SPHKAP isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK127071.1, CR749494.1, BC104954.1, AB051465.1 and AK095042.1. On Dec 13, 2008 this sequence version replaced NM_030623.2. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1, and encodes a shorter isoform (2) than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.25617.1, SRR1803615.178383.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGGCA ACTCCCTGCT CTCGGTACCA AGCAACTTGG AGTCATCACG GATGTATGAC 
GTTTTGGAAC CGCAGCAGGG CAGAGGCTGT GGCAGCTCAG GAAGCGGCCC GGGGAACTCC 
ATCACAGCCT GTAAGAAGGT TCTTCGCAGC AATAGCCTGC TGGAGTCAAC AGACTACTGG 
TTGCAGAATC AGAGGATGCC CTGCCAAATT GGTTTTGTAG AAGACAAGTC TGAAAACTGT 
GCTTCTGTCT GCTTTGTGAA TCTTGATGTG AACAAGGATG AATGCAGCAC AGAGCACCTG 
CAACAGAAAC TGGTCAACGT TTCACCAGAT CTTCCAAAAC TTATCAGTTC CATGAATGTC 
CAACAACCAA AAGAAAATGA AATTGTTGTC CTAAGTGGGT TAGCCTCTGG AAATCTCCAG 
GCAGATTTTG AAGTTTCACA GTGCCCTTGG CTGCCAGATA TCTGCTTGGT CCAATGTGCA 
AGAGGGAACA GACCAAACAG TACCAACTGC ATCATCTTTG AAATCAACAA ATTTCTGATT 
GGTCTGGAAC TGGTGCAGGA GCGACAGCTC CACCTGGAAA CAAACATCTT GAAACTGGAG 
GATGACACGA ACTGTTCCTT ATCTTCAATC GAGGAAGACT TTCTCACCGC TTCTGAGCAC 
TTGGAGGAGG AAAGCGAGGT GGATGAATCT AGGAACGATT ATGAAAATAT AAATGTCTCA 
GCCAATGTTT TGGAAAGTAA ACAGCTAAAG GGAGCCACCC AGGTGGAATG GAATTGCAAC 
AAGGAAAAGT GGCTTTATGC TTTGGAAGAC AAATACATCA ACAAATATCC CACACCATTG 
ATTAAAACAG AACGATCTCC AGAAAACCTA ACAAAGAACA CAGCCTTGCA GAGTCTAGAT 
CCCTCAGCCA AGCCATCACA GTGGAAAAGA GAAGCTGTGG GGAATGGGAG ACAAGCCACA 
CATTATTATC ATTCAGAAGC TTTTAAAGGT CAAATGGAAA AATCACAGGC ACTGTATATT 
CCAAAAGATG CTTATTTCTC CATGATGGAT AAAGATGTAC CTTCTGCATG TGCTGTGGCA 
GAGCAGAGAA GCAACCTAAA CCCAGGAGAC CATGAAGACA CAAGAAACGC TCTCCCTCCT 
AGACAAGATG GAGAAGTCAC CACTGGCAAG TATGCTACAA ATTTAGCAGA ATCCGTGCTG 
CAGGATGCAT TTATTAGATT ATCTCAATCT CAGTCCACAT TACCCCAGGA ATCTGCAGTC 
AGTGTTTCTG TAGGAAGTTC TCTGCTTCCC AGTTGCTATT CCACAAAAGA TACAGTGGTT 
TCTCGGTCAT GGAATGAGCT CCCCAAAATC GTCGTTGTTC AGAGTCCAGA TGGCAGTGAT 
GCTGCCCCAC AGCCAGGCAT CTCCTCCTGG CCTGAGATGG AAGTCTCTGT TGAAACCTCA 
AGCATCCTCT CTGGAGAGAA CTCCAGCAGA CAACCCCAGA GTGCTCTAGA AGTGGCGTTA 
GCTTGTGCAG CCACTGTGAT TGGAACTATT TCCAGTCCAC AGGCCACAGA AAGACTCAAA 
ATGGAGCAAG TGGTCTCGAA CTTTCCCCCA GGGAGCAGTG GTGCACTGCA AACTCAAGCA 
CCCCAAGGAC TCAAGGAACC TTCCATCAAT GAGTACTCCT TTCCATCTGC TTTGTGTGGC 
ATGACTCAGG TGGCCAGTGC CGTGGCTGTC TGTGGTCTGG GTGAAAGAGA AGAGGTGACA 
TGCTCAGTGG CTCCAAGTGG TAGCCTCCCG CCTGCAGCTG AGGCTTCTGA AGCCATGCCC 
CCACTTTGTG GTTTAGCAAG CATGGAGCTT GGCAAGGAAG CCATTGCCAA GGGATTGCTC 
AAGGAGGCTG CTCTGGTTTT AACAAGGCCT AATACCTACA GCAGCATTGG AGACTTTCTG 
GACTCCATGA ACAGGAGAAT CATGGAAACT GCTTCAAAGT CTCAGACCCT GTGCTCAGAA 
AATGTCGTCA GGAATGAACT GGCACATACC CTGTCCAATG TTATCCTGAG GCATTCCATT 
GATGAAGTTC ACCACAAAAA TATGATAATC GACCCCAATG ACAACAGGCA TTCATCTGAA 
ATTCTGGACA CCTTAATGGA AAGTACAAAT CAACTGCTTT TAGATGTGAT ATGCTTCACG 
TTCAAGAAGA TGAGTCATAT TGTACGGCTT GGTGAATGTC CTGCTGTCCT TTCTAAGGAG 
ACCATCAGAA GGAGGGAGAC AGAACCAAGC TGCCAGCCAT CTGATCCGGG TGCTAGTCAA 
GCTTGGACAA AAGCCACTGA ATCCTCCAGC AGCTCTCCAC TTAGCAATTC ACACAACACG 
AGTCTTGTCA TCAACAATCT TGTGGATGGC ATGTATTCAA AACAAGACAA GGGTGGAGTG 
AGGCCAGGCC TCTTCAAGAA CCCCACGCTG CAGTCACAAT TATCACGTAG TCACAGAGTG 
CCCGATTCTT CAACTGCTAC AACATCCTCC AAGGAAATAT ATCTGAAAGG AATAGCAGGA 
GAGGATACAA AAAGCCCTCA TCACAGTGAG AATGAATGCA GAGCCTCTTC CGAAGGACAA 
AGGTCCCCAA CGGTCAGCCA GTCCAGAAGT GGTTCCCAGG AGGCTGAGGA GAGTATCCAC 
CCAAACACCC AAGAAAAGTA CAACTGTGCC ACATCTCGCA TCAACGAAGT TCAAGTCAAC 
CTGTCCTTGT TAGGGGATGA CCTGCTGCTT CCTGCTCAAT CCACGCTTCA AACAAAGCAT 
CCAGACATCT ACTGCATTAC AGACTTTGCG GAAGAATTAG CAGACACGGT CGTCTCCATG 
GCAACTGAAA TTGCAGCGAT TTGCCTTGAC AACTCCAGTG GAAAACAACC CTGGTTTTGT 
GCATGGAAAA GAGGGAGTGA GTTTCTGATG ACACCCAACG TACCCTGCCG ATCCTTGAAG 
AGGAAGAAAG AGAGCCAGGG GAGCGGGACC GCTGTGAGGA AACACAAGCC TCCCCGGCTC 
AGTGAGATCA AGAGGAAGAC GGACGAGCAC CCTGAGCTTA AAGAAAAGCT GATGAACAGG 
GTTGTGGATG AGTCCATGAA CCTTGAAGAT GTCCCAGATT CTGTCAATCT TTTTGCCAAT 
GAAGTGGCAG CCAAGATCAT GAACCTAACG GAGTTCTCTA TGGTGGACGG CATGTGGCAG 
GCGCAGGGCT ATCCCCGGAA TCGGTTACTG AGTGGCGACA GGTGGAGCCG GCTGAAGGCC 
TCCAGCTGCG AAAGCATTCC TGAGGAAGAC TCCGAGGCCA GGGCCTATGT CAACAGCCTG 
GGCTTAATGA GCACGCTGAG CCAGCCGGTC AGCAGGGCCA GCTCTGTCTC CAAGCAGTCG 
AGCTGTGAGA GCATCACCGA TGAGTTTTCC AGGTTCATGG TGAACCAGAT GGAAAATGAA 
GGGAGAGGAT TTGAGTTACT GCTGGATTAC TATGCTGGCA AGAACGCCAG CAGCATTCTG 
AACTCAGCCA TGCAACAGGC GTGCCGGAAA AGTGACCACC TCAGTGTGAG GCCTAGCTGT 
CCCTCTAAGC AGTCCAGCAC AGAGAGCATC ACTGAGGAGT TCTACAGGTA CATGCTGAGG 
GACATCGAAA GAGACAGCAG AGAAAGTGCC TCCTCCAGAC GGAGCAGCCA GGATTGGACA 
GCCGGCCTGC TGTCTCCTTC TCTGCGATCC CCAGTGTGCC ACAGACAGTC GTCCATGCCA 
GACAGCAGAT CCCCATGCTC CAGGCTGACA GTGAATGTGC CCATCAAAGC CAACTCTTTA 
GATGGCTTTG CTCAGAACTG CCCACAAGAT TTCCTAAGCG TGCAGCCGGT CAGTAGCGCG 
TCCTCATCCG GTCTCTGCAA ATCTGACTCT TGCTTGTATC GGAGAGGTGG GACTGACCAC 
ATCACCAACA TGTTAATTCA TGAAACGTGG GCTAGCTCCA TTGAGGCTCT CATGCGCAAG 
AACAAAATCA TTGTGGATGA TGCAGAGGAA GCTGACACTG AGCCTGTTTC TGGTGGCTCT 
CCCTCGCAAG CAGAGAAGTG TGCAAATAGA TTAGCTGCGA GCAGGATGTG CAGTGGGCCA 
ACTCTGCTTG TTCAGGAGTC TCTCGATTGC CCGAGGAAAG ACTCTGTTAC CGAATGTAAA 
CAGCCCCCAG TGTCATCTTT GAGCAAAACT GCTTCTCTTA CAAACCACAG CCCTTTAGAT 
TCTAAAAAAG AAACTTCCTC GTGCCAGGAC CCTGTACCAA TAAACCACAA AAGGCGATCA 
CTTTGCTCGA GGGAAGTGCC TTTGATTCAG ATTGAAACAG ATCAGAGAGA AGCCTGTGCT 
GGGGAACCTG AACCCTTCCT TTCCAAAAGC AGCCTCCTAG AGGAAGCAGA AGGGCATTCG 
AATGACAAAA ACATCCCAGA TGTGGTGAGA GGTGGAGACA CAGCCGTGAG CGCTTGTCAA 
ATCCATAGTG ACAGCCTTGA TACCAGAGAT GTACCAGAGG CTGAAGCCTC CACAGAAGCC 
AGAGCCCCCG ATGAGGCCCC CAACCCTCCA AGCAGCAGCG AGGAGAGCAC AGGCAGCTGG 
ACCCAGCTTG CCAATGAGGA AGACAACCCA GATGACACAA GTAGCTTTCT CCAGCTCAGT 
GAGCGATCCA TGAGTGAATT AGTAGAAGAA AAGAAGATTC TTAAAGGACA GTCAGAAAGC 
ACAGAGGCAC CTGCATCTGG ACCGCCTACG GGAACAGCCA GCCCCCAGAG GAGCCTGCTG 
GTGATCAACT TTGACCTGGA GCCAGAGTGT CCAGATGCCG AGCTCCGAGC CACTCTGCAG 
TGGATAGCTG CCTCTGAACT GGGGATTCCC ACCATCTACT TTAAGAAATC TCAGGAAAAC 
AGAATTGAAA AGTTTCTAGA TGTCGTGCAG CTGGTTCATC GGAAGTCCTG GAAAGTGGGT 
GATATCTTCC ATGCAGTTGT CCAGTACTGC AAAATGCATG AGGAGCAGAA GGATGGGAGA 
CTGAGTCTCT TTGACTGGCT CTTGGAACTG GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085126.2
CDS	48..5063
Translation	MDGNSLLSVPSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQIGFVEDKSENCASVCFVNLDVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSIEEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPLIKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMDKDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAVSVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSILSGENSSRQPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCGMTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRPNTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSEILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSSSSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAGEDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLLPAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLKRKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLTEFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERDSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSLDGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEEADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLDSKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVRGGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLSERSMSELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCKMHEEQKDGRLSLFDWLLELG

Target ORF information:

RefSeq Version	NM_030623.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030623.3

ORF Insert Sequence:

ATGGATGGCA ACTCCCTGCT CTCGGTACCA AGCAACTTGG AGTCATCACG GATGTATGAC 
GTTTTGGAAC CGCAGCAGGG CAGAGGCTGT GGCAGCTCAG GAAGCGGCCC GGGGAACTCC 
ATCACAGCCT GTAAGAAGGT TCTTCGCAGC AATAGCCTGC TGGAGTCAAC AGACTACTGG 
TTGCAGAATC AGAGGATGCC CTGCCAAATT GGTTTTGTAG AAGACAAGTC TGAAAACTGT 
GCTTCTGTCT GCTTTGTGAA TCTTGATGTG AACAAGGATG AATGCAGCAC AGAGCACCTG 
CAACAGAAAC TGGTCAACGT TTCACCAGAT CTTCCAAAAC TTATCAGTTC CATGAATGTC 
CAACAACCAA AAGAAAATGA AATTGTTGTC CTAAGTGGGT TAGCCTCTGG AAATCTCCAG 
GCAGATTTTG AAGTTTCACA GTGCCCTTGG CTGCCAGATA TCTGCTTGGT CCAATGTGCA 
AGAGGGAACA GACCAAACAG TACCAACTGC ATCATCTTTG AAATCAACAA ATTTCTGATT 
GGTCTGGAAC TGGTGCAGGA GCGACAGCTC CACCTGGAAA CAAACATCTT GAAACTGGAG 
GATGACACGA ACTGTTCCTT ATCTTCAATC GAGGAAGACT TTCTCACCGC TTCTGAGCAC 
TTGGAGGAGG AAAGCGAGGT GGATGAATCT AGGAACGATT ATGAAAATAT AAATGTCTCA 
GCCAATGTTT TGGAAAGTAA ACAGCTAAAG GGAGCCACCC AGGTGGAATG GAATTGCAAC 
AAGGAAAAGT GGCTTTATGC TTTGGAAGAC AAATACATCA ACAAATATCC CACACCATTG 
ATTAAAACAG AACGATCTCC AGAAAACCTA ACAAAGAACA CAGCCTTGCA GAGTCTAGAT 
CCCTCAGCCA AGCCATCACA GTGGAAAAGA GAAGCTGTGG GGAATGGGAG ACAAGCCACA 
CATTATTATC ATTCAGAAGC TTTTAAAGGT CAAATGGAAA AATCACAGGC ACTGTATATT 
CCAAAAGATG CTTATTTCTC CATGATGGAT AAAGATGTAC CTTCTGCATG TGCTGTGGCA 
GAGCAGAGAA GCAACCTAAA CCCAGGAGAC CATGAAGACA CAAGAAACGC TCTCCCTCCT 
AGACAAGATG GAGAAGTCAC CACTGGCAAG TATGCTACAA ATTTAGCAGA ATCCGTGCTG 
CAGGATGCAT TTATTAGATT ATCTCAATCT CAGTCCACAT TACCCCAGGA ATCTGCAGTC 
AGTGTTTCTG TAGGAAGTTC TCTGCTTCCC AGTTGCTATT CCACAAAAGA TACAGTGGTT 
TCTCGGTCAT GGAATGAGCT CCCCAAAATC GTCGTTGTTC AGAGTCCAGA TGGCAGTGAT 
GCTGCCCCAC AGCCAGGCAT CTCCTCCTGG CCTGAGATGG AAGTCTCTGT TGAAACCTCA 
AGCATCCTCT CTGGAGAGAA CTCCAGCAGA CAACCCCAGA GTGCTCTAGA AGTGGCGTTA 
GCTTGTGCAG CCACTGTGAT TGGAACTATT TCCAGTCCAC AGGCCACAGA AAGACTCAAA 
ATGGAGCAAG TGGTCTCGAA CTTTCCCCCA GGGAGCAGTG GTGCACTGCA AACTCAAGCA 
CCCCAAGGAC TCAAGGAACC TTCCATCAAT GAGTACTCCT TTCCATCTGC TTTGTGTGGC 
ATGACTCAGG TGGCCAGTGC CGTGGCTGTC TGTGGTCTGG GTGAAAGAGA AGAGGTGACA 
TGCTCAGTGG CTCCAAGTGG TAGCCTCCCG CCTGCAGCTG AGGCTTCTGA AGCCATGCCC 
CCACTTTGTG GTTTAGCAAG CATGGAGCTT GGCAAGGAAG CCATTGCCAA GGGATTGCTC 
AAGGAGGCTG CTCTGGTTTT AACAAGGCCT AATACCTACA GCAGCATTGG AGACTTTCTG 
GACTCCATGA ACAGGAGAAT CATGGAAACT GCTTCAAAGT CTCAGACCCT GTGCTCAGAA 
AATGTCGTCA GGAATGAACT GGCACATACC CTGTCCAATG TTATCCTGAG GCATTCCATT 
GATGAAGTTC ACCACAAAAA TATGATAATC GACCCCAATG ACAACAGGCA TTCATCTGAA 
ATTCTGGACA CCTTAATGGA AAGTACAAAT CAACTGCTTT TAGATGTGAT ATGCTTCACG 
TTCAAGAAGA TGAGTCATAT TGTACGGCTT GGTGAATGTC CTGCTGTCCT TTCTAAGGAG 
ACCATCAGAA GGAGGGAGAC AGAACCAAGC TGCCAGCCAT CTGATCCGGG TGCTAGTCAA 
GCTTGGACAA AAGCCACTGA ATCCTCCAGC AGCTCTCCAC TTAGCAATTC ACACAACACG 
AGTCTTGTCA TCAACAATCT TGTGGATGGC ATGTATTCAA AACAAGACAA GGGTGGAGTG 
AGGCCAGGCC TCTTCAAGAA CCCCACGCTG CAGTCACAAT TATCACGTAG TCACAGAGTG 
CCCGATTCTT CAACTGCTAC AACATCCTCC AAGGAAATAT ATCTGAAAGG AATAGCAGGA 
GAGGATACAA AAAGCCCTCA TCACAGTGAG AATGAATGCA GAGCCTCTTC CGAAGGACAA 
AGGTCCCCAA CGGTCAGCCA GTCCAGAAGT GGTTCCCAGG AGGCTGAGGA GAGTATCCAC 
CCAAACACCC AAGAAAAGTA CAACTGTGCC ACATCTCGCA TCAACGAAGT TCAAGTCAAC 
CTGTCCTTGT TAGGGGATGA CCTGCTGCTT CCTGCTCAAT CCACGCTTCA AACAAAGCAT 
CCAGACATCT ACTGCATTAC AGACTTTGCG GAAGAATTAG CAGACACGGT CGTCTCCATG 
GCAACTGAAA TTGCAGCGAT TTGCCTTGAC AACTCCAGTG GAAAACAACC CTGGTTTTGT 
GCATGGAAAA GAGGGAGTGA GTTTCTGATG ACACCCAACG TACCCTGCCG ATCCTTGAAG 
AGGAAGAAAG AGAGCCAGGG GAGCGGGACC GCTGTGAGGA AACACAAGCC TCCCCGGCTC 
AGTGAGATCA AGAGGAAGAC GGACGAGCAC CCTGAGCTTA AAGAAAAGCT GATGAACAGG 
GTTGTGGATG AGTCCATGAA CCTTGAAGAT GTCCCAGATT CTGTCAATCT TTTTGCCAAT 
GAAGTGGCAG CCAAGATCAT GAACCTAACG GAGTTCTCTA TGGTGGACGG CATGTGGCAG 
GCGCAGGGCT ATCCCCGGAA TCGGTTACTG AGTGGCGACA GGTGGAGCCG GCTGAAGGCC 
TCCAGCTGCG AAAGCATTCC TGAGGAAGAC TCCGAGGCCA GGGCCTATGT CAACAGCCTG 
GGCTTAATGA GCACGCTGAG CCAGCCGGTC AGCAGGGCCA GCTCTGTCTC CAAGCAGTCG 
AGCTGTGAGA GCATCACCGA TGAGTTTTCC AGGTTCATGG TGAACCAGAT GGAAAATGAA 
GGGAGAGGAT TTGAGTTACT GCTGGATTAC TATGCTGGCA AGAACGCCAG CAGCATTCTG 
AACTCAGCCA TGCAACAGGC GTGCCGGAAA AGTGACCACC TCAGTGTGAG GCCTAGCTGT 
CCCTCTAAGC AGTCCAGCAC AGAGAGCATC ACTGAGGAGT TCTACAGGTA CATGCTGAGG 
GACATCGAAA GAGACAGCAG AGAAAGTGCC TCCTCCAGAC GGAGCAGCCA GGATTGGACA 
GCCGGCCTGC TGTCTCCTTC TCTGCGATCC CCAGTGTGCC ACAGACAGTC GTCCATGCCA 
GACAGCAGAT CCCCATGCTC CAGGCTGACA GTGAATGTGC CCATCAAAGC CAACTCTTTA 
GATGGCTTTG CTCAGAACTG CCCACAAGAT TTCCTAAGCG TGCAGCCGGT CAGTAGCGCG 
TCCTCATCCG GTCTCTGCAA ATCTGACTCT TGCTTGTATC GGAGAGGTGG GACTGACCAC 
ATCACCAACA TGTTAATTCA TGAAACGTGG GCTAGCTCCA TTGAGGCTCT CATGCGCAAG 
AACAAAATCA TTGTGGATGA TGCAGAGGAA GCTGACACTG AGCCTGTTTC TGGTGGCTCT 
CCCTCGCAAG CAGAGAAGTG TGCAAATAGA TTAGCTGCGA GCAGGATGTG CAGTGGGCCA 
ACTCTGCTTG TTCAGGAGTC TCTCGATTGC CCGAGGAAAG ACTCTGTTAC CGAATGTAAA 
CAGCCCCCAG TGTCATCTTT GAGCAAAACT GCTTCTCTTA CAAACCACAG CCCTTTAGAT 
TCTAAAAAAG AAACTTCCTC GTGCCAGGAC CCTGTACCAA TAAACCACAA AAGGCGATCA 
CTTTGCTCGA GGGAAGTGCC TTTGATTCAG ATTGAAACAG ATCAGAGAGA AGCCTGTGCT 
GGGGAACCTG AACCCTTCCT TTCCAAAAGC AGCCTCCTAG AGGAAGCAGA AGGGCATTCG 
AATGACAAAA ACATCCCAGA TGTGGTGAGA GGTGGAGACA CAGCCGTGAG CGCTTGTCAA 
ATCCATAGTG ACAGCCTTGA TACCAGAGAT GTACCAGAGG CTGAAGCCTC CACAGAAGCC 
AGAGCCCCCG ATGAGGCCCC CAACCCTCCA AGCAGCAGCG AGGAGAGCAC AGGCAGCTGG 
ACCCAGCTTG CCAATGAGGA AGACAACCCA GATGACACAA GTAGCTTTCT CCAGCTCAGT 
GAGCGATCCA TGAGTGAATT AGTAGAAGAA AAGAAGATTC TTAAAGGACA GTCAGAAAGC 
ACAGAGGCAC CTGCATCTGG ACCGCCTACG GGAACAGCCA GCCCCCAGAG GAGCCTGCTG 
GTGATCAACT TTGACCTGGA GCCAGAGTGT CCAGATGCCG AGCTCCGAGC CACTCTGCAG 
TGGATAGCTG CCTCTGAACT GGGGATTCCC ACCATCTACT TTAAGAAATC TCAGGAAAAC 
AGAATTGAAA AGTTTCTAGA TGTCGTGCAG CTGGTTCATC GGAAGTCCTG GAAAGTGGGT 
GATATCTTCC ATGCAGTTGT CCAGTACTGC AAAATGCATG AGGAGCAGAA GGATGGGAGA 
CTGAGTCTCT TTGACTGGCT CTTGGAACTG GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_006712777.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712840.1 Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

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CloneID	OHu36648
Clone ID Related Accession (Same CDS sequence)	XM_006712777.3
Accession Version	XM_006712777.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712777.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CCTGCTCTCG GTACCAAGCC ATGTGGGTAG TTGAACCAAA CTCACCTACC 
TGCCTCTCTG CTGCCTTGCC CACCAGGAGA CACTCCCAGG AATCCAGCAA CTTGGAGTCA 
TCACGGATGT ATGACGTTTT GGAACCGCAG CAGGGCAGAG GCTGTGGCAG CTCAGGAAGC 
GGCCCGGGGA ACTCCATCAC AGCCTGTAAG AAGGTTCTTC GCAGCAATAG CCTGCTGGAG 
TCAACAGACT ACTGGTTGCA GAATCAGAGG ATGCCCTGCC AAATTGGTTT TGTAGAAGAC 
AAGTCTGAAA ACTGTGCTTC TGTCTGCTTT GTGAATCTTG ATGTGAACAA GGATGAATGC 
AGCACAGAGC ACCTGCAACA GAAACTGGTC AACGTTTCAC CAGATCTTCC AAAACTTATC 
AGTTCCATGA ATGTCCAACA ACCAAAAGAA AATGAAATTG TTGTCCTAAG TGGGTTAGCC 
TCTGGAAATC TCCAGGCAGA TTTTGAAGTT TCACAGTGCC CTTGGCTGCC AGATATCTGC 
TTGGTCCAAT GTGCAAGAGG GAACAGACCA AACAGTACCA ACTGCATCAT CTTTGAAATC 
AACAAATTTC TGATTGGTCT GGAACTGGTG CAGGAGCGAC AGCTCCACCT GGAAACAAAC 
ATCTTGAAAC TGGAGGATGA CACGAACTGT TCCTTATCTT CAATCGAGGA AGACTTTCTC 
ACCGCTTCTG AGCACTTGGA GGAGGAAAGC GAGGTGGATG AATCTAGGAA CGATTATGAA 
AATATAAATG TCTCAGCCAA TGTTTTGGAA AGTAAACAGC TAAAGGGAGC CACCCAGGTG 
GAATGGAATT GCAACAAGGA AAAGTGGCTT TATGCTTTGG AAGACAAATA CATCAACAAA 
TATCCCACAC CATTGATTAA AACAGAACGA TCTCCAGAAA ACCTAACAAA GAACACAGCC 
TTGCAGAGTC TAGATCCCTC AGCCAAGCCA TCACAGTGGA AAAGAGAAGC TGTGGGGAAT 
GGGAGACAAG CCACACATTA TTATCATTCA GAAGCTTTTA AAGGTCAAAT GGAAAAATCA 
CAGGCACTGT ATATTCCAAA AGATGCTTAT TTCTCCATGA TGGATAAAGA TGTACCTTCT 
GCATGTGCTG TGGCAGAGCA GAGAAGCAAC CTAAACCCAG GAGACCATGA AGACACAAGA 
AACGCTCTCC CTCCTAGACA AGATGGAGAA GTCACCACTG GCAAGTATGC TACAAATTTA 
GCAGAATCCG TGCTGCAGGA TGCATTTATT AGATTATCTC AATCTCAGTC CACATTACCC 
CAGGAATCTG CAGTCAGTGT TTCTGTAGGA AGTTCTCTGC TTCCCAGTTG CTATTCCACA 
AAAGATACAG TGGTTTCTCG GTCATGGAAT GAGCTCCCCA AAATCGTCGT TGTTCAGAGT 
CCAGATGGCA GTGATGCTGC CCCACAGCCA GGCATCTCCT CCTGGCCTGA GATGGAAGTC 
TCTGTTGAAA CCTCAAGCAT CCTCTCTGGA GAGAACTCCA GCAGACAACC CCAGAGTGCT 
CTAGAAGTGG CGTTAGCTTG TGCAGCCACT GTGATTGGAA CTATTTCCAG TCCACAGGCC 
ACAGAAAGAC TCAAAATGGA GCAAGTGGTC TCGAACTTTC CCCCAGGGAG CAGTGGTGCA 
CTGCAAACTC AAGCACCCCA AGGACTCAAG GAACCTTCCA TCAATGAGTA CTCCTTTCCA 
TCTGCTTTGT GTGGCATGAC TCAGGTGGCC AGTGCCGTGG CTGTCTGTGG TCTGGGTGAA 
AGAGAAGAGG TGACATGCTC AGTGGCTCCA AGTGGTAGCC TCCCGCCTGC AGCTGAGGCT 
TCTGAAGCCA TGCCCCCACT TTGTGGTTTA GCAAGCATGG AGCTTGGCAA GGAAGCCATT 
GCCAAGGGAT TGCTCAAGGA GGCTGCTCTG GTTTTAACAA GGCCTAATAC CTACAGCAGC 
ATTGGAGACT TTCTGGACTC CATGAACAGG AGAATCATGG AAACTGCTTC AAAGTCTCAG 
ACCCTGTGCT CAGAAAATGT CGTCAGGAAT GAACTGGCAC ATACCCTGTC CAATGTTATC 
CTGAGGCATT CCATTGATGA AGTTCACCAC AAAAATATGA TAATCGACCC CAATGACAAC 
AGGCATTCAT CTGAAATTCT GGACACCTTA ATGGAAAGTA CAAATCAACT GCTTTTAGAT 
GTGATATGCT TCACGTTCAA GAAGATGAGT CATATTGTAC GGCTTGGTGA ATGTCCTGCT 
GTCCTTTCTA AGGAGACCAT CAGAAGGAGG GAGACAGAAC CAAGCTGCCA GCCATCTGAT 
CCGGGTGCTA GTCAAGCTTG GACAAAAGCC ACTGAATCCT CCAGCAGCTC TCCACTTAGC 
AATTCACACA ACACGAGTCT TGTCATCAAC AATCTTGTGG ATGGCATGTA TTCAAAACAA 
GACAAGGGTG GAGTGAGGCC AGGCCTCTTC AAGAACCCCA CGCTGCAGTC ACAATTATCA 
CGTAGTCACA GAGTGCCCGA TTCTTCAACT GCTACAACAT CCTCCAAGGA AATATATCTG 
AAAGGAATAG CAGGAGAGGA TACAAAAAGC CCTCATCACA GTGAGAATGA ATGCAGAGCC 
TCTTCCGAAG GACAAAGGTC CCCAACGGTC AGCCAGTCCA GAAGTGGTTC CCAGGAGGCT 
GAGGAGAGTA TCCACCCAAA CACCCAAGAA AAGTACAACT GTGCCACATC TCGCATCAAC 
GAAGTTCAAG TCAACCTGTC CTTGTTAGGG GATGACCTGC TGCTTCCTGC TCAATCCACG 
CTTCAAACAA AGCATCCAGA CATCTACTGC ATTACAGACT TTGCGGAAGA ATTAGCAGAC 
ACGGTCGTCT CCATGGCAAC TGAAATTGCA GCGATTTGCC TTGACAACTC CAGTGGAAAA 
CAACCCTGGT TTTGTGCATG GAAAAGAGGG AGTGAGTTTC TGATGACACC CAACGTACCC 
TGCCGATCCT TGAAGAGGAA GAAAGAGAGC CAGGGGAGCG GGACCGCTGT GAGGAAACAC 
AAGCCTCCCC GGCTCAGTGA GATCAAGAGG AAGACGGACG AGCACCCTGA GCTTAAAGAA 
AAGCTGATGA ACAGGGTTGT GGATGAGTCC ATGAACCTTG AAGATGTCCC AGATTCTGTC 
AATCTTTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TAACGGAGTT CTCTATGGTG 
GACGGCATGT GGCAGGCGCA GGGCTATCCC CGGAATCGGT TACTGAGTGG CGACAGGTGG 
AGCCGGCTGA AGGCCTCCAG CTGCGAAAGC ATTCCTGAGG AAGACTCCGA GGCCAGGGCC 
TATGTCAACA GCCTGGGCTT AATGAGCACG CTGAGCCAGC CGGTCAGCAG GGCCAGCTCT 
GTCTCCAAGC AGTCGAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAC 
CAGATGGAAA ATGAAGGGAG AGGATTTGAG TTACTGCTGG ATTACTATGC TGGCAAGAAC 
GCCAGCAGCA TTCTGAACTC AGCCATGCAA CAGGCGTGCC GGAAAAGTGA CCACCTCAGT 
GTGAGGCCTA GCTGTCCCTC TAAGCAGTCC AGCACAGAGA GCATCACTGA GGAGTTCTAC 
AGGTACATGC TGAGGGACAT CGAAAGAGAC AGCAGAGAAA GTGCCTCCTC CAGACGGAGC 
AGCCAGGATT GGACAGCCGG CCTGCTGTCT CCTTCTCTGC GATCCCCAGT GTGCCACAGA 
CAGTCGTCCA TGCCAGACAG CAGATCCCCA TGCTCCAGGC TGACAGTGAA TGTGCCCATC 
AAAGCCAACT CTTTAGATGG CTTTGCTCAG AACTGCCCAC AAGATTTCCT AAGCGTGCAG 
CCGGTCAGTA GCGCGTCCTC ATCCGGTCTC TGCAAATCTG ACTCTTGCTT GTATCGGAGA 
GGTGGGACTG ACCACATCAC CAACATGTTA ATTCATGAAA CGTGGGCTAG CTCCATTGAG 
GCTCTCATGC GCAAGAACAA AATCATTGTG GATGATGCAG AGGAAGCTGA CACTGAGCCT 
GTTTCTGGTG GCTCTCCCTC GCAAGCAGAG AAGTGTGCAA ATAGATTAGC TGCGAGCAGG 
ATGTGCAGTG GGCCAACTCT GCTTGTTCAG GAGTCTCTCG ATTGCCCGAG GAAAGACTCT 
GTTACCGAAT GTAAACAGCC CCCAGTGTCA TCTTTGAGCA AAACTGCTTC TCTTACAAAC 
CACAGCCCTT TAGATTCTAA AAAAGAAACT TCCTCGTGCC AGGACCCTGT ACCAATAAAC 
CACAAAAGGC GATCACTTTG CTCGAGGGAA GTGCCTTTGA TTCAGATTGA AACAGATCAG 
AGAGAAGCCT GTGCTGGGGA ACCTGAACCC TTCCTTTCCA AAAGCAGCCT CCTAGAGGAA 
GCAGAAGGGC ATTCGAATGA CAAAAACATC CCAGATGTGG TGAGAGGTGG AGACACAGCC 
GTGAGCGCTT GTCAAATCCA TAGTGACAGC CTTGATACCA GAGATGTACC AGAGGCTGAA 
GCCTCCACAG AAGCCAGAGC CCCCGATGAG GCCCCCAACC CTCCAAGCAG CAGCGAGGAG 
AGCACAGGCA GCTGGACCCA GCTTGCCAAT GAGGAAGACA ACCCAGATGA CACAAGTAGC 
TTTCTCCAGC TCAGTGAGCG ATCCATGAGC AATGGCAACA GTAGTGCCAC TAGCAGTCTT 
GGCATTATGG ATCTGGACAT TTATCAGGAA AGCATGCCAT CTTCTCCCAT GATTAATGAA 
TTAGTAGAAG AAAAGAAGAT TCTTAAAGGA CAGTCAGAAA GCACAGAGGC ACCTGCATCT 
GGACCGCCTA CGGGAACAGC CAGCCCCCAG AGGAGCCTGC TGGTGATCAA CTTTGACCTG 
GAGCCAGAGT GTCCAGATGC CGAGCTCCGA GCCACTCTGC AGTGGATAGC TGCCTCTGAA 
CTGGGGATTC CCACCATCTA CTTTAAGAAA TCTCAGGAAA ACAGAATTGA AAAGTTTCTA 
GATGTCGTGC AGCTGGTTCA TCGGAAGTCC TGGAAAGTGG GTGATATCTT CCATGCAGTT 
GTCCAGTACT GCAAAATGCA TGAGGAGCAG AAGGATGGGA GACTGAGTCT CTTTGACTGG 
CTCTTGGAAC TGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712840.1
CDS	406..5583
Translation	MATPCSRYQAMWVVEPNSPTCLSAALPTRRHSQESSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQIGFVEDKSENCASVCFVNLDVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSIEEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPLIKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMDKDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAVSVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSILSGENSSRQPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCGMTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRPNTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSEILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSSSSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAGEDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLLPAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLKRKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLTEFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERDSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSLDGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEEADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLDSKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVRGGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLSERSMSNGNSSATSSLGIMDLDIYQESMPSSPMINELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCKMHEEQKDGRLSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006712777.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712777.3

ORF Insert Sequence:

ATGGCAACTC CCTGCTCTCG GTACCAAGCC ATGTGGGTAG TTGAACCAAA CTCACCTACC 
TGCCTCTCTG CTGCCTTGCC CACCAGGAGA CACTCCCAGG AATCCAGCAA CTTGGAGTCA 
TCACGGATGT ATGACGTTTT GGAACCGCAG CAGGGCAGAG GCTGTGGCAG CTCAGGAAGC 
GGCCCGGGGA ACTCCATCAC AGCCTGTAAG AAGGTTCTTC GCAGCAATAG CCTGCTGGAG 
TCAACAGACT ACTGGTTGCA GAATCAGAGG ATGCCCTGCC AAATTGGTTT TGTAGAAGAC 
AAGTCTGAAA ACTGTGCTTC TGTCTGCTTT GTGAATCTTG ATGTGAACAA GGATGAATGC 
AGCACAGAGC ACCTGCAACA GAAACTGGTC AACGTTTCAC CAGATCTTCC AAAACTTATC 
AGTTCCATGA ATGTCCAACA ACCAAAAGAA AATGAAATTG TTGTCCTAAG TGGGTTAGCC 
TCTGGAAATC TCCAGGCAGA TTTTGAAGTT TCACAGTGCC CTTGGCTGCC AGATATCTGC 
TTGGTCCAAT GTGCAAGAGG GAACAGACCA AACAGTACCA ACTGCATCAT CTTTGAAATC 
AACAAATTTC TGATTGGTCT GGAACTGGTG CAGGAGCGAC AGCTCCACCT GGAAACAAAC 
ATCTTGAAAC TGGAGGATGA CACGAACTGT TCCTTATCTT CAATCGAGGA AGACTTTCTC 
ACCGCTTCTG AGCACTTGGA GGAGGAAAGC GAGGTGGATG AATCTAGGAA CGATTATGAA 
AATATAAATG TCTCAGCCAA TGTTTTGGAA AGTAAACAGC TAAAGGGAGC CACCCAGGTG 
GAATGGAATT GCAACAAGGA AAAGTGGCTT TATGCTTTGG AAGACAAATA CATCAACAAA 
TATCCCACAC CATTGATTAA AACAGAACGA TCTCCAGAAA ACCTAACAAA GAACACAGCC 
TTGCAGAGTC TAGATCCCTC AGCCAAGCCA TCACAGTGGA AAAGAGAAGC TGTGGGGAAT 
GGGAGACAAG CCACACATTA TTATCATTCA GAAGCTTTTA AAGGTCAAAT GGAAAAATCA 
CAGGCACTGT ATATTCCAAA AGATGCTTAT TTCTCCATGA TGGATAAAGA TGTACCTTCT 
GCATGTGCTG TGGCAGAGCA GAGAAGCAAC CTAAACCCAG GAGACCATGA AGACACAAGA 
AACGCTCTCC CTCCTAGACA AGATGGAGAA GTCACCACTG GCAAGTATGC TACAAATTTA 
GCAGAATCCG TGCTGCAGGA TGCATTTATT AGATTATCTC AATCTCAGTC CACATTACCC 
CAGGAATCTG CAGTCAGTGT TTCTGTAGGA AGTTCTCTGC TTCCCAGTTG CTATTCCACA 
AAAGATACAG TGGTTTCTCG GTCATGGAAT GAGCTCCCCA AAATCGTCGT TGTTCAGAGT 
CCAGATGGCA GTGATGCTGC CCCACAGCCA GGCATCTCCT CCTGGCCTGA GATGGAAGTC 
TCTGTTGAAA CCTCAAGCAT CCTCTCTGGA GAGAACTCCA GCAGACAACC CCAGAGTGCT 
CTAGAAGTGG CGTTAGCTTG TGCAGCCACT GTGATTGGAA CTATTTCCAG TCCACAGGCC 
ACAGAAAGAC TCAAAATGGA GCAAGTGGTC TCGAACTTTC CCCCAGGGAG CAGTGGTGCA 
CTGCAAACTC AAGCACCCCA AGGACTCAAG GAACCTTCCA TCAATGAGTA CTCCTTTCCA 
TCTGCTTTGT GTGGCATGAC TCAGGTGGCC AGTGCCGTGG CTGTCTGTGG TCTGGGTGAA 
AGAGAAGAGG TGACATGCTC AGTGGCTCCA AGTGGTAGCC TCCCGCCTGC AGCTGAGGCT 
TCTGAAGCCA TGCCCCCACT TTGTGGTTTA GCAAGCATGG AGCTTGGCAA GGAAGCCATT 
GCCAAGGGAT TGCTCAAGGA GGCTGCTCTG GTTTTAACAA GGCCTAATAC CTACAGCAGC 
ATTGGAGACT TTCTGGACTC CATGAACAGG AGAATCATGG AAACTGCTTC AAAGTCTCAG 
ACCCTGTGCT CAGAAAATGT CGTCAGGAAT GAACTGGCAC ATACCCTGTC CAATGTTATC 
CTGAGGCATT CCATTGATGA AGTTCACCAC AAAAATATGA TAATCGACCC CAATGACAAC 
AGGCATTCAT CTGAAATTCT GGACACCTTA ATGGAAAGTA CAAATCAACT GCTTTTAGAT 
GTGATATGCT TCACGTTCAA GAAGATGAGT CATATTGTAC GGCTTGGTGA ATGTCCTGCT 
GTCCTTTCTA AGGAGACCAT CAGAAGGAGG GAGACAGAAC CAAGCTGCCA GCCATCTGAT 
CCGGGTGCTA GTCAAGCTTG GACAAAAGCC ACTGAATCCT CCAGCAGCTC TCCACTTAGC 
AATTCACACA ACACGAGTCT TGTCATCAAC AATCTTGTGG ATGGCATGTA TTCAAAACAA 
GACAAGGGTG GAGTGAGGCC AGGCCTCTTC AAGAACCCCA CGCTGCAGTC ACAATTATCA 
CGTAGTCACA GAGTGCCCGA TTCTTCAACT GCTACAACAT CCTCCAAGGA AATATATCTG 
AAAGGAATAG CAGGAGAGGA TACAAAAAGC CCTCATCACA GTGAGAATGA ATGCAGAGCC 
TCTTCCGAAG GACAAAGGTC CCCAACGGTC AGCCAGTCCA GAAGTGGTTC CCAGGAGGCT 
GAGGAGAGTA TCCACCCAAA CACCCAAGAA AAGTACAACT GTGCCACATC TCGCATCAAC 
GAAGTTCAAG TCAACCTGTC CTTGTTAGGG GATGACCTGC TGCTTCCTGC TCAATCCACG 
CTTCAAACAA AGCATCCAGA CATCTACTGC ATTACAGACT TTGCGGAAGA ATTAGCAGAC 
ACGGTCGTCT CCATGGCAAC TGAAATTGCA GCGATTTGCC TTGACAACTC CAGTGGAAAA 
CAACCCTGGT TTTGTGCATG GAAAAGAGGG AGTGAGTTTC TGATGACACC CAACGTACCC 
TGCCGATCCT TGAAGAGGAA GAAAGAGAGC CAGGGGAGCG GGACCGCTGT GAGGAAACAC 
AAGCCTCCCC GGCTCAGTGA GATCAAGAGG AAGACGGACG AGCACCCTGA GCTTAAAGAA 
AAGCTGATGA ACAGGGTTGT GGATGAGTCC ATGAACCTTG AAGATGTCCC AGATTCTGTC 
AATCTTTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TAACGGAGTT CTCTATGGTG 
GACGGCATGT GGCAGGCGCA GGGCTATCCC CGGAATCGGT TACTGAGTGG CGACAGGTGG 
AGCCGGCTGA AGGCCTCCAG CTGCGAAAGC ATTCCTGAGG AAGACTCCGA GGCCAGGGCC 
TATGTCAACA GCCTGGGCTT AATGAGCACG CTGAGCCAGC CGGTCAGCAG GGCCAGCTCT 
GTCTCCAAGC AGTCGAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAC 
CAGATGGAAA ATGAAGGGAG AGGATTTGAG TTACTGCTGG ATTACTATGC TGGCAAGAAC 
GCCAGCAGCA TTCTGAACTC AGCCATGCAA CAGGCGTGCC GGAAAAGTGA CCACCTCAGT 
GTGAGGCCTA GCTGTCCCTC TAAGCAGTCC AGCACAGAGA GCATCACTGA GGAGTTCTAC 
AGGTACATGC TGAGGGACAT CGAAAGAGAC AGCAGAGAAA GTGCCTCCTC CAGACGGAGC 
AGCCAGGATT GGACAGCCGG CCTGCTGTCT CCTTCTCTGC GATCCCCAGT GTGCCACAGA 
CAGTCGTCCA TGCCAGACAG CAGATCCCCA TGCTCCAGGC TGACAGTGAA TGTGCCCATC 
AAAGCCAACT CTTTAGATGG CTTTGCTCAG AACTGCCCAC AAGATTTCCT AAGCGTGCAG 
CCGGTCAGTA GCGCGTCCTC ATCCGGTCTC TGCAAATCTG ACTCTTGCTT GTATCGGAGA 
GGTGGGACTG ACCACATCAC CAACATGTTA ATTCATGAAA CGTGGGCTAG CTCCATTGAG 
GCTCTCATGC GCAAGAACAA AATCATTGTG GATGATGCAG AGGAAGCTGA CACTGAGCCT 
GTTTCTGGTG GCTCTCCCTC GCAAGCAGAG AAGTGTGCAA ATAGATTAGC TGCGAGCAGG 
ATGTGCAGTG GGCCAACTCT GCTTGTTCAG GAGTCTCTCG ATTGCCCGAG GAAAGACTCT 
GTTACCGAAT GTAAACAGCC CCCAGTGTCA TCTTTGAGCA AAACTGCTTC TCTTACAAAC 
CACAGCCCTT TAGATTCTAA AAAAGAAACT TCCTCGTGCC AGGACCCTGT ACCAATAAAC 
CACAAAAGGC GATCACTTTG CTCGAGGGAA GTGCCTTTGA TTCAGATTGA AACAGATCAG 
AGAGAAGCCT GTGCTGGGGA ACCTGAACCC TTCCTTTCCA AAAGCAGCCT CCTAGAGGAA 
GCAGAAGGGC ATTCGAATGA CAAAAACATC CCAGATGTGG TGAGAGGTGG AGACACAGCC 
GTGAGCGCTT GTCAAATCCA TAGTGACAGC CTTGATACCA GAGATGTACC AGAGGCTGAA 
GCCTCCACAG AAGCCAGAGC CCCCGATGAG GCCCCCAACC CTCCAAGCAG CAGCGAGGAG 
AGCACAGGCA GCTGGACCCA GCTTGCCAAT GAGGAAGACA ACCCAGATGA CACAAGTAGC 
TTTCTCCAGC TCAGTGAGCG ATCCATGAGC AATGGCAACA GTAGTGCCAC TAGCAGTCTT 
GGCATTATGG ATCTGGACAT TTATCAGGAA AGCATGCCAT CTTCTCCCAT GATTAATGAA 
TTAGTAGAAG AAAAGAAGAT TCTTAAAGGA CAGTCAGAAA GCACAGAGGC ACCTGCATCT 
GGACCGCCTA CGGGAACAGC CAGCCCCCAG AGGAGCCTGC TGGTGATCAA CTTTGACCTG 
GAGCCAGAGT GTCCAGATGC CGAGCTCCGA GCCACTCTGC AGTGGATAGC TGCCTCTGAA 
CTGGGGATTC CCACCATCTA CTTTAAGAAA TCTCAGGAAA ACAGAATTGA AAAGTTTCTA 
GATGTCGTGC AGCTGGTTCA TCGGAAGTCC TGGAAAGTGG GTGATATCTT CCATGCAGTT 
GTCCAGTACT GCAAAATGCA TGAGGAGCAG AAGGATGGGA GACTGAGTCT CTTTGACTGG 
CTCTTGGAAC TGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_006712778.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712841.1 Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.

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CloneID	OHu36650
Clone ID Related Accession (Same CDS sequence)	XM_006712778.3 , XM_006712778.4
Accession Version	XM_006712778.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	A-kinase anchor protein SPHKAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712778.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CCTGCTCTCG GTACCAAGCC ATGTGGGTAG TTGAACCAAA CTCACCTACC 
TGCCTCTCTG CTGCCTTGCC CACCAGGAGA CACTCCCAGG AATCCAGCAA CTTGGAGTCA 
TCACGGATGT ATGACGTTTT GGAACCGCAG CAGGGCAGAG GCTGTGGCAG CTCAGGAAGC 
GGCCCGGGGA ACTCCATCAC AGCCTGTAAG AAGGTTCTTC GCAGCAATAG CCTGCTGGAG 
TCAACAGACT ACTGGTTGCA GAATCAGAGG ATGCCCTGCC AAATTGGTTT TGTAGAAGAC 
AAGTCTGAAA ACTGTGCTTC TGTCTGCTTT GTGAATCTTG ATGTGAACAA GGATGAATGC 
AGCACAGAGC ACCTGCAACA GAAACTGGTC AACGTTTCAC CAGATCTTCC AAAACTTATC 
AGTTCCATGA ATGTCCAACA ACCAAAAGAA AATGAAATTG TTGTCCTAAG TGGGTTAGCC 
TCTGGAAATC TCCAGGCAGA TTTTGAAGTT TCACAGTGCC CTTGGCTGCC AGATATCTGC 
TTGGTCCAAT GTGCAAGAGG GAACAGACCA AACAGTACCA ACTGCATCAT CTTTGAAATC 
AACAAATTTC TGATTGGTCT GGAACTGGTG CAGGAGCGAC AGCTCCACCT GGAAACAAAC 
ATCTTGAAAC TGGAGGATGA CACGAACTGT TCCTTATCTT CAATCGAGGA AGACTTTCTC 
ACCGCTTCTG AGCACTTGGA GGAGGAAAGC GAGGTGGATG AATCTAGGAA CGATTATGAA 
AATATAAATG TCTCAGCCAA TGTTTTGGAA AGTAAACAGC TAAAGGGAGC CACCCAGGTG 
GAATGGAATT GCAACAAGGA AAAGTGGCTT TATGCTTTGG AAGACAAATA CATCAACAAA 
TATCCCACAC CATTGATTAA AACAGAACGA TCTCCAGAAA ACCTAACAAA GAACACAGCC 
TTGCAGAGTC TAGATCCCTC AGCCAAGCCA TCACAGTGGA AAAGAGAAGC TGTGGGGAAT 
GGGAGACAAG CCACACATTA TTATCATTCA GAAGCTTTTA AAGGTCAAAT GGAAAAATCA 
CAGGCACTGT ATATTCCAAA AGATGCTTAT TTCTCCATGA TGGATAAAGA TGTACCTTCT 
GCATGTGCTG TGGCAGAGCA GAGAAGCAAC CTAAACCCAG GAGACCATGA AGACACAAGA 
AACGCTCTCC CTCCTAGACA AGATGGAGAA GTCACCACTG GCAAGTATGC TACAAATTTA 
GCAGAATCCG TGCTGCAGGA TGCATTTATT AGATTATCTC AATCTCAGTC CACATTACCC 
CAGGAATCTG CAGTCAGTGT TTCTGTAGGA AGTTCTCTGC TTCCCAGTTG CTATTCCACA 
AAAGATACAG TGGTTTCTCG GTCATGGAAT GAGCTCCCCA AAATCGTCGT TGTTCAGAGT 
CCAGATGGCA GTGATGCTGC CCCACAGCCA GGCATCTCCT CCTGGCCTGA GATGGAAGTC 
TCTGTTGAAA CCTCAAGCAT CCTCTCTGGA GAGAACTCCA GCAGACAACC CCAGAGTGCT 
CTAGAAGTGG CGTTAGCTTG TGCAGCCACT GTGATTGGAA CTATTTCCAG TCCACAGGCC 
ACAGAAAGAC TCAAAATGGA GCAAGTGGTC TCGAACTTTC CCCCAGGGAG CAGTGGTGCA 
CTGCAAACTC AAGCACCCCA AGGACTCAAG GAACCTTCCA TCAATGAGTA CTCCTTTCCA 
TCTGCTTTGT GTGGCATGAC TCAGGTGGCC AGTGCCGTGG CTGTCTGTGG TCTGGGTGAA 
AGAGAAGAGG TGACATGCTC AGTGGCTCCA AGTGGTAGCC TCCCGCCTGC AGCTGAGGCT 
TCTGAAGCCA TGCCCCCACT TTGTGGTTTA GCAAGCATGG AGCTTGGCAA GGAAGCCATT 
GCCAAGGGAT TGCTCAAGGA GGCTGCTCTG GTTTTAACAA GGCCTAATAC CTACAGCAGC 
ATTGGAGACT TTCTGGACTC CATGAACAGG AGAATCATGG AAACTGCTTC AAAGTCTCAG 
ACCCTGTGCT CAGAAAATGT CGTCAGGAAT GAACTGGCAC ATACCCTGTC CAATGTTATC 
CTGAGGCATT CCATTGATGA AGTTCACCAC AAAAATATGA TAATCGACCC CAATGACAAC 
AGGCATTCAT CTGAAATTCT GGACACCTTA ATGGAAAGTA CAAATCAACT GCTTTTAGAT 
GTGATATGCT TCACGTTCAA GAAGATGAGT CATATTGTAC GGCTTGGTGA ATGTCCTGCT 
GTCCTTTCTA AGGAGACCAT CAGAAGGAGG GAGACAGAAC CAAGCTGCCA GCCATCTGAT 
CCGGGTGCTA GTCAAGCTTG GACAAAAGCC ACTGAATCCT CCAGCAGCTC TCCACTTAGC 
AATTCACACA ACACGAGTCT TGTCATCAAC AATCTTGTGG ATGGCATGTA TTCAAAACAA 
GACAAGGGTG GAGTGAGGCC AGGCCTCTTC AAGAACCCCA CGCTGCAGTC ACAATTATCA 
CGTAGTCACA GAGTGCCCGA TTCTTCAACT GCTACAACAT CCTCCAAGGA AATATATCTG 
AAAGGAATAG CAGGAGAGGA TACAAAAAGC CCTCATCACA GTGAGAATGA ATGCAGAGCC 
TCTTCCGAAG GACAAAGGTC CCCAACGGTC AGCCAGTCCA GAAGTGGTTC CCAGGAGGCT 
GAGGAGAGTA TCCACCCAAA CACCCAAGAA AAGTACAACT GTGCCACATC TCGCATCAAC 
GAAGTTCAAG TCAACCTGTC CTTGTTAGGG GATGACCTGC TGCTTCCTGC TCAATCCACG 
CTTCAAACAA AGCATCCAGA CATCTACTGC ATTACAGACT TTGCGGAAGA ATTAGCAGAC 
ACGGTCGTCT CCATGGCAAC TGAAATTGCA GCGATTTGCC TTGACAACTC CAGTGGAAAA 
CAACCCTGGT TTTGTGCATG GAAAAGAGGG AGTGAGTTTC TGATGACACC CAACGTACCC 
TGCCGATCCT TGAAGAGGAA GAAAGAGAGC CAGGGGAGCG GGACCGCTGT GAGGAAACAC 
AAGCCTCCCC GGCTCAGTGA GATCAAGAGG AAGACGGACG AGCACCCTGA GCTTAAAGAA 
AAGCTGATGA ACAGGGTTGT GGATGAGTCC ATGAACCTTG AAGATGTCCC AGATTCTGTC 
AATCTTTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TAACGGAGTT CTCTATGGTG 
GACGGCATGT GGCAGGCGCA GGGCTATCCC CGGAATCGGT TACTGAGTGG CGACAGGTGG 
AGCCGGCTGA AGGCCTCCAG CTGCGAAAGC ATTCCTGAGG AAGACTCCGA GGCCAGGGCC 
TATGTCAACA GCCTGGGCTT AATGAGCACG CTGAGCCAGC CGGTCAGCAG GGCCAGCTCT 
GTCTCCAAGC AGTCGAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAC 
CAGATGGAAA ATGAAGGGAG AGGATTTGAG TTACTGCTGG ATTACTATGC TGGCAAGAAC 
GCCAGCAGCA TTCTGAACTC AGCCATGCAA CAGGCGTGCC GGAAAAGTGA CCACCTCAGT 
GTGAGGCCTA GCTGTCCCTC TAAGCAGTCC AGCACAGAGA GCATCACTGA GGAGTTCTAC 
AGGTACATGC TGAGGGACAT CGAAAGAGAC AGCAGAGAAA GTGCCTCCTC CAGACGGAGC 
AGCCAGGATT GGACAGCCGG CCTGCTGTCT CCTTCTCTGC GATCCCCAGT GTGCCACAGA 
CAGTCGTCCA TGCCAGACAG CAGATCCCCA TGCTCCAGGC TGACAGTGAA TGTGCCCATC 
AAAGCCAACT CTTTAGATGG CTTTGCTCAG AACTGCCCAC AAGATTTCCT AAGCGTGCAG 
CCGGTCAGTA GCGCGTCCTC ATCCGGTCTC TGCAAATCTG ACTCTTGCTT GTATCGGAGA 
GGTGGGACTG ACCACATCAC CAACATGTTA ATTCATGAAA CGTGGGCTAG CTCCATTGAG 
GCTCTCATGC GCAAGAACAA AATCATTGTG GATGATGCAG AGGAAGCTGA CACTGAGCCT 
GTTTCTGGTG GCTCTCCCTC GCAAGCAGAG AAGTGTGCAA ATAGATTAGC TGCGAGCAGG 
ATGTGCAGTG GGCCAACTCT GCTTGTTCAG GAGTCTCTCG ATTGCCCGAG GAAAGACTCT 
GTTACCGAAT GTAAACAGCC CCCAGTGTCA TCTTTGAGCA AAACTGCTTC TCTTACAAAC 
CACAGCCCTT TAGATTCTAA AAAAGAAACT TCCTCGTGCC AGGACCCTGT ACCAATAAAC 
CACAAAAGGC GATCACTTTG CTCGAGGGAA GTGCCTTTGA TTCAGATTGA AACAGATCAG 
AGAGAAGCCT GTGCTGGGGA ACCTGAACCC TTCCTTTCCA AAAGCAGCCT CCTAGAGGAA 
GCAGAAGGGC ATTCGAATGA CAAAAACATC CCAGATGTGG TGAGAGGTGG AGACACAGCC 
GTGAGCGCTT GTCAAATCCA TAGTGACAGC CTTGATACCA GAGATGTACC AGAGGCTGAA 
GCCTCCACAG AAGCCAGAGC CCCCGATGAG GCCCCCAACC CTCCAAGCAG CAGCGAGGAG 
AGCACAGGCA GCTGGACCCA GCTTGCCAAT GAGGAAGACA ACCCAGATGA CACAAGTAGC 
TTTCTCCAGC TCAGTGAGCG ATCCATGAGT GAATTAGTAG AAGAAAAGAA GATTCTTAAA 
GGACAGTCAG AAAGCACAGA GGCACCTGCA TCTGGACCGC CTACGGGAAC AGCCAGCCCC 
CAGAGGAGCC TGCTGGTGAT CAACTTTGAC CTGGAGCCAG AGTGTCCAGA TGCCGAGCTC 
CGAGCCACTC TGCAGTGGAT AGCTGCCTCT GAACTGGGGA TTCCCACCAT CTACTTTAAG 
AAATCTCAGG AAAACAGAAT TGAAAAGTTT CTAGATGTCG TGCAGCTGGT TCATCGGAAG 
TCCTGGAAAG TGGGTGATAT CTTCCATGCA GTTGTCCAGT ACTGCAAAAT GCATGAGGAG 
CAGAAGGATG GGAGACTGAG TCTCTTTGAC TGGCTCTTGG AACTGGGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712841.1
CDS	405..5495
Translation	MATPCSRYQAMWVVEPNSPTCLSAALPTRRHSQESSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQIGFVEDKSENCASVCFVNLDVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSIEEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPLIKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMDKDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAVSVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSILSGENSSRQPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCGMTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRPNTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSEILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSSSSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAGEDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLLPAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLKRKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLTEFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERDSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSLDGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEEADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLDSKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVRGGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLSERSMSELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCKMHEEQKDGRLSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006712778.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712778.3

ORF Insert Sequence:

ATGGCAACTC CCTGCTCTCG GTACCAAGCC ATGTGGGTAG TTGAACCAAA CTCACCTACC 
TGCCTCTCTG CTGCCTTGCC CACCAGGAGA CACTCCCAGG AATCCAGCAA CTTGGAGTCA 
TCACGGATGT ATGACGTTTT GGAACCGCAG CAGGGCAGAG GCTGTGGCAG CTCAGGAAGC 
GGCCCGGGGA ACTCCATCAC AGCCTGTAAG AAGGTTCTTC GCAGCAATAG CCTGCTGGAG 
TCAACAGACT ACTGGTTGCA GAATCAGAGG ATGCCCTGCC AAATTGGTTT TGTAGAAGAC 
AAGTCTGAAA ACTGTGCTTC TGTCTGCTTT GTGAATCTTG ATGTGAACAA GGATGAATGC 
AGCACAGAGC ACCTGCAACA GAAACTGGTC AACGTTTCAC CAGATCTTCC AAAACTTATC 
AGTTCCATGA ATGTCCAACA ACCAAAAGAA AATGAAATTG TTGTCCTAAG TGGGTTAGCC 
TCTGGAAATC TCCAGGCAGA TTTTGAAGTT TCACAGTGCC CTTGGCTGCC AGATATCTGC 
TTGGTCCAAT GTGCAAGAGG GAACAGACCA AACAGTACCA ACTGCATCAT CTTTGAAATC 
AACAAATTTC TGATTGGTCT GGAACTGGTG CAGGAGCGAC AGCTCCACCT GGAAACAAAC 
ATCTTGAAAC TGGAGGATGA CACGAACTGT TCCTTATCTT CAATCGAGGA AGACTTTCTC 
ACCGCTTCTG AGCACTTGGA GGAGGAAAGC GAGGTGGATG AATCTAGGAA CGATTATGAA 
AATATAAATG TCTCAGCCAA TGTTTTGGAA AGTAAACAGC TAAAGGGAGC CACCCAGGTG 
GAATGGAATT GCAACAAGGA AAAGTGGCTT TATGCTTTGG AAGACAAATA CATCAACAAA 
TATCCCACAC CATTGATTAA AACAGAACGA TCTCCAGAAA ACCTAACAAA GAACACAGCC 
TTGCAGAGTC TAGATCCCTC AGCCAAGCCA TCACAGTGGA AAAGAGAAGC TGTGGGGAAT 
GGGAGACAAG CCACACATTA TTATCATTCA GAAGCTTTTA AAGGTCAAAT GGAAAAATCA 
CAGGCACTGT ATATTCCAAA AGATGCTTAT TTCTCCATGA TGGATAAAGA TGTACCTTCT 
GCATGTGCTG TGGCAGAGCA GAGAAGCAAC CTAAACCCAG GAGACCATGA AGACACAAGA 
AACGCTCTCC CTCCTAGACA AGATGGAGAA GTCACCACTG GCAAGTATGC TACAAATTTA 
GCAGAATCCG TGCTGCAGGA TGCATTTATT AGATTATCTC AATCTCAGTC CACATTACCC 
CAGGAATCTG CAGTCAGTGT TTCTGTAGGA AGTTCTCTGC TTCCCAGTTG CTATTCCACA 
AAAGATACAG TGGTTTCTCG GTCATGGAAT GAGCTCCCCA AAATCGTCGT TGTTCAGAGT 
CCAGATGGCA GTGATGCTGC CCCACAGCCA GGCATCTCCT CCTGGCCTGA GATGGAAGTC 
TCTGTTGAAA CCTCAAGCAT CCTCTCTGGA GAGAACTCCA GCAGACAACC CCAGAGTGCT 
CTAGAAGTGG CGTTAGCTTG TGCAGCCACT GTGATTGGAA CTATTTCCAG TCCACAGGCC 
ACAGAAAGAC TCAAAATGGA GCAAGTGGTC TCGAACTTTC CCCCAGGGAG CAGTGGTGCA 
CTGCAAACTC AAGCACCCCA AGGACTCAAG GAACCTTCCA TCAATGAGTA CTCCTTTCCA 
TCTGCTTTGT GTGGCATGAC TCAGGTGGCC AGTGCCGTGG CTGTCTGTGG TCTGGGTGAA 
AGAGAAGAGG TGACATGCTC AGTGGCTCCA AGTGGTAGCC TCCCGCCTGC AGCTGAGGCT 
TCTGAAGCCA TGCCCCCACT TTGTGGTTTA GCAAGCATGG AGCTTGGCAA GGAAGCCATT 
GCCAAGGGAT TGCTCAAGGA GGCTGCTCTG GTTTTAACAA GGCCTAATAC CTACAGCAGC 
ATTGGAGACT TTCTGGACTC CATGAACAGG AGAATCATGG AAACTGCTTC AAAGTCTCAG 
ACCCTGTGCT CAGAAAATGT CGTCAGGAAT GAACTGGCAC ATACCCTGTC CAATGTTATC 
CTGAGGCATT CCATTGATGA AGTTCACCAC AAAAATATGA TAATCGACCC CAATGACAAC 
AGGCATTCAT CTGAAATTCT GGACACCTTA ATGGAAAGTA CAAATCAACT GCTTTTAGAT 
GTGATATGCT TCACGTTCAA GAAGATGAGT CATATTGTAC GGCTTGGTGA ATGTCCTGCT 
GTCCTTTCTA AGGAGACCAT CAGAAGGAGG GAGACAGAAC CAAGCTGCCA GCCATCTGAT 
CCGGGTGCTA GTCAAGCTTG GACAAAAGCC ACTGAATCCT CCAGCAGCTC TCCACTTAGC 
AATTCACACA ACACGAGTCT TGTCATCAAC AATCTTGTGG ATGGCATGTA TTCAAAACAA 
GACAAGGGTG GAGTGAGGCC AGGCCTCTTC AAGAACCCCA CGCTGCAGTC ACAATTATCA 
CGTAGTCACA GAGTGCCCGA TTCTTCAACT GCTACAACAT CCTCCAAGGA AATATATCTG 
AAAGGAATAG CAGGAGAGGA TACAAAAAGC CCTCATCACA GTGAGAATGA ATGCAGAGCC 
TCTTCCGAAG GACAAAGGTC CCCAACGGTC AGCCAGTCCA GAAGTGGTTC CCAGGAGGCT 
GAGGAGAGTA TCCACCCAAA CACCCAAGAA AAGTACAACT GTGCCACATC TCGCATCAAC 
GAAGTTCAAG TCAACCTGTC CTTGTTAGGG GATGACCTGC TGCTTCCTGC TCAATCCACG 
CTTCAAACAA AGCATCCAGA CATCTACTGC ATTACAGACT TTGCGGAAGA ATTAGCAGAC 
ACGGTCGTCT CCATGGCAAC TGAAATTGCA GCGATTTGCC TTGACAACTC CAGTGGAAAA 
CAACCCTGGT TTTGTGCATG GAAAAGAGGG AGTGAGTTTC TGATGACACC CAACGTACCC 
TGCCGATCCT TGAAGAGGAA GAAAGAGAGC CAGGGGAGCG GGACCGCTGT GAGGAAACAC 
AAGCCTCCCC GGCTCAGTGA GATCAAGAGG AAGACGGACG AGCACCCTGA GCTTAAAGAA 
AAGCTGATGA ACAGGGTTGT GGATGAGTCC ATGAACCTTG AAGATGTCCC AGATTCTGTC 
AATCTTTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TAACGGAGTT CTCTATGGTG 
GACGGCATGT GGCAGGCGCA GGGCTATCCC CGGAATCGGT TACTGAGTGG CGACAGGTGG 
AGCCGGCTGA AGGCCTCCAG CTGCGAAAGC ATTCCTGAGG AAGACTCCGA GGCCAGGGCC 
TATGTCAACA GCCTGGGCTT AATGAGCACG CTGAGCCAGC CGGTCAGCAG GGCCAGCTCT 
GTCTCCAAGC AGTCGAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAC 
CAGATGGAAA ATGAAGGGAG AGGATTTGAG TTACTGCTGG ATTACTATGC TGGCAAGAAC 
GCCAGCAGCA TTCTGAACTC AGCCATGCAA CAGGCGTGCC GGAAAAGTGA CCACCTCAGT 
GTGAGGCCTA GCTGTCCCTC TAAGCAGTCC AGCACAGAGA GCATCACTGA GGAGTTCTAC 
AGGTACATGC TGAGGGACAT CGAAAGAGAC AGCAGAGAAA GTGCCTCCTC CAGACGGAGC 
AGCCAGGATT GGACAGCCGG CCTGCTGTCT CCTTCTCTGC GATCCCCAGT GTGCCACAGA 
CAGTCGTCCA TGCCAGACAG CAGATCCCCA TGCTCCAGGC TGACAGTGAA TGTGCCCATC 
AAAGCCAACT CTTTAGATGG CTTTGCTCAG AACTGCCCAC AAGATTTCCT AAGCGTGCAG 
CCGGTCAGTA GCGCGTCCTC ATCCGGTCTC TGCAAATCTG ACTCTTGCTT GTATCGGAGA 
GGTGGGACTG ACCACATCAC CAACATGTTA ATTCATGAAA CGTGGGCTAG CTCCATTGAG 
GCTCTCATGC GCAAGAACAA AATCATTGTG GATGATGCAG AGGAAGCTGA CACTGAGCCT 
GTTTCTGGTG GCTCTCCCTC GCAAGCAGAG AAGTGTGCAA ATAGATTAGC TGCGAGCAGG 
ATGTGCAGTG GGCCAACTCT GCTTGTTCAG GAGTCTCTCG ATTGCCCGAG GAAAGACTCT 
GTTACCGAAT GTAAACAGCC CCCAGTGTCA TCTTTGAGCA AAACTGCTTC TCTTACAAAC 
CACAGCCCTT TAGATTCTAA AAAAGAAACT TCCTCGTGCC AGGACCCTGT ACCAATAAAC 
CACAAAAGGC GATCACTTTG CTCGAGGGAA GTGCCTTTGA TTCAGATTGA AACAGATCAG 
AGAGAAGCCT GTGCTGGGGA ACCTGAACCC TTCCTTTCCA AAAGCAGCCT CCTAGAGGAA 
GCAGAAGGGC ATTCGAATGA CAAAAACATC CCAGATGTGG TGAGAGGTGG AGACACAGCC 
GTGAGCGCTT GTCAAATCCA TAGTGACAGC CTTGATACCA GAGATGTACC AGAGGCTGAA 
GCCTCCACAG AAGCCAGAGC CCCCGATGAG GCCCCCAACC CTCCAAGCAG CAGCGAGGAG 
AGCACAGGCA GCTGGACCCA GCTTGCCAAT GAGGAAGACA ACCCAGATGA CACAAGTAGC 
TTTCTCCAGC TCAGTGAGCG ATCCATGAGT GAATTAGTAG AAGAAAAGAA GATTCTTAAA 
GGACAGTCAG AAAGCACAGA GGCACCTGCA TCTGGACCGC CTACGGGAAC AGCCAGCCCC 
CAGAGGAGCC TGCTGGTGAT CAACTTTGAC CTGGAGCCAG AGTGTCCAGA TGCCGAGCTC 
CGAGCCACTC TGCAGTGGAT AGCTGCCTCT GAACTGGGGA TTCCCACCAT CTACTTTAAG 
AAATCTCAGG AAAACAGAAT TGAAAAGTTT CTAGATGTCG TGCAGCTGGT TCATCGGAAG 
TCCTGGAAAG TGGGTGATAT CTTCCATGCA GTTGTCCAGT ACTGCAAAAT GCATGAGGAG 
CAGAAGGATG GGAGACTGAG TCTCTTTGAC TGGCTCTTGG AACTGGGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_006712778.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712841.1 Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.

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CloneID	OHu36650
Clone ID Related Accession (Same CDS sequence)	XM_006712778.3 , XM_006712778.4
Accession Version	XM_006712778.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	A-kinase anchor protein SPHKAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006712778.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CCTGCTCTCG GTACCAAGCC ATGTGGGTAG TTGAACCAAA CTCACCTACC 
TGCCTCTCTG CTGCCTTGCC CACCAGGAGA CACTCCCAGG AATCCAGCAA CTTGGAGTCA 
TCACGGATGT ATGACGTTTT GGAACCGCAG CAGGGCAGAG GCTGTGGCAG CTCAGGAAGC 
GGCCCGGGGA ACTCCATCAC AGCCTGTAAG AAGGTTCTTC GCAGCAATAG CCTGCTGGAG 
TCAACAGACT ACTGGTTGCA GAATCAGAGG ATGCCCTGCC AAATTGGTTT TGTAGAAGAC 
AAGTCTGAAA ACTGTGCTTC TGTCTGCTTT GTGAATCTTG ATGTGAACAA GGATGAATGC 
AGCACAGAGC ACCTGCAACA GAAACTGGTC AACGTTTCAC CAGATCTTCC AAAACTTATC 
AGTTCCATGA ATGTCCAACA ACCAAAAGAA AATGAAATTG TTGTCCTAAG TGGGTTAGCC 
TCTGGAAATC TCCAGGCAGA TTTTGAAGTT TCACAGTGCC CTTGGCTGCC AGATATCTGC 
TTGGTCCAAT GTGCAAGAGG GAACAGACCA AACAGTACCA ACTGCATCAT CTTTGAAATC 
AACAAATTTC TGATTGGTCT GGAACTGGTG CAGGAGCGAC AGCTCCACCT GGAAACAAAC 
ATCTTGAAAC TGGAGGATGA CACGAACTGT TCCTTATCTT CAATCGAGGA AGACTTTCTC 
ACCGCTTCTG AGCACTTGGA GGAGGAAAGC GAGGTGGATG AATCTAGGAA CGATTATGAA 
AATATAAATG TCTCAGCCAA TGTTTTGGAA AGTAAACAGC TAAAGGGAGC CACCCAGGTG 
GAATGGAATT GCAACAAGGA AAAGTGGCTT TATGCTTTGG AAGACAAATA CATCAACAAA 
TATCCCACAC CATTGATTAA AACAGAACGA TCTCCAGAAA ACCTAACAAA GAACACAGCC 
TTGCAGAGTC TAGATCCCTC AGCCAAGCCA TCACAGTGGA AAAGAGAAGC TGTGGGGAAT 
GGGAGACAAG CCACACATTA TTATCATTCA GAAGCTTTTA AAGGTCAAAT GGAAAAATCA 
CAGGCACTGT ATATTCCAAA AGATGCTTAT TTCTCCATGA TGGATAAAGA TGTACCTTCT 
GCATGTGCTG TGGCAGAGCA GAGAAGCAAC CTAAACCCAG GAGACCATGA AGACACAAGA 
AACGCTCTCC CTCCTAGACA AGATGGAGAA GTCACCACTG GCAAGTATGC TACAAATTTA 
GCAGAATCCG TGCTGCAGGA TGCATTTATT AGATTATCTC AATCTCAGTC CACATTACCC 
CAGGAATCTG CAGTCAGTGT TTCTGTAGGA AGTTCTCTGC TTCCCAGTTG CTATTCCACA 
AAAGATACAG TGGTTTCTCG GTCATGGAAT GAGCTCCCCA AAATCGTCGT TGTTCAGAGT 
CCAGATGGCA GTGATGCTGC CCCACAGCCA GGCATCTCCT CCTGGCCTGA GATGGAAGTC 
TCTGTTGAAA CCTCAAGCAT CCTCTCTGGA GAGAACTCCA GCAGACAACC CCAGAGTGCT 
CTAGAAGTGG CGTTAGCTTG TGCAGCCACT GTGATTGGAA CTATTTCCAG TCCACAGGCC 
ACAGAAAGAC TCAAAATGGA GCAAGTGGTC TCGAACTTTC CCCCAGGGAG CAGTGGTGCA 
CTGCAAACTC AAGCACCCCA AGGACTCAAG GAACCTTCCA TCAATGAGTA CTCCTTTCCA 
TCTGCTTTGT GTGGCATGAC TCAGGTGGCC AGTGCCGTGG CTGTCTGTGG TCTGGGTGAA 
AGAGAAGAGG TGACATGCTC AGTGGCTCCA AGTGGTAGCC TCCCGCCTGC AGCTGAGGCT 
TCTGAAGCCA TGCCCCCACT TTGTGGTTTA GCAAGCATGG AGCTTGGCAA GGAAGCCATT 
GCCAAGGGAT TGCTCAAGGA GGCTGCTCTG GTTTTAACAA GGCCTAATAC CTACAGCAGC 
ATTGGAGACT TTCTGGACTC CATGAACAGG AGAATCATGG AAACTGCTTC AAAGTCTCAG 
ACCCTGTGCT CAGAAAATGT CGTCAGGAAT GAACTGGCAC ATACCCTGTC CAATGTTATC 
CTGAGGCATT CCATTGATGA AGTTCACCAC AAAAATATGA TAATCGACCC CAATGACAAC 
AGGCATTCAT CTGAAATTCT GGACACCTTA ATGGAAAGTA CAAATCAACT GCTTTTAGAT 
GTGATATGCT TCACGTTCAA GAAGATGAGT CATATTGTAC GGCTTGGTGA ATGTCCTGCT 
GTCCTTTCTA AGGAGACCAT CAGAAGGAGG GAGACAGAAC CAAGCTGCCA GCCATCTGAT 
CCGGGTGCTA GTCAAGCTTG GACAAAAGCC ACTGAATCCT CCAGCAGCTC TCCACTTAGC 
AATTCACACA ACACGAGTCT TGTCATCAAC AATCTTGTGG ATGGCATGTA TTCAAAACAA 
GACAAGGGTG GAGTGAGGCC AGGCCTCTTC AAGAACCCCA CGCTGCAGTC ACAATTATCA 
CGTAGTCACA GAGTGCCCGA TTCTTCAACT GCTACAACAT CCTCCAAGGA AATATATCTG 
AAAGGAATAG CAGGAGAGGA TACAAAAAGC CCTCATCACA GTGAGAATGA ATGCAGAGCC 
TCTTCCGAAG GACAAAGGTC CCCAACGGTC AGCCAGTCCA GAAGTGGTTC CCAGGAGGCT 
GAGGAGAGTA TCCACCCAAA CACCCAAGAA AAGTACAACT GTGCCACATC TCGCATCAAC 
GAAGTTCAAG TCAACCTGTC CTTGTTAGGG GATGACCTGC TGCTTCCTGC TCAATCCACG 
CTTCAAACAA AGCATCCAGA CATCTACTGC ATTACAGACT TTGCGGAAGA ATTAGCAGAC 
ACGGTCGTCT CCATGGCAAC TGAAATTGCA GCGATTTGCC TTGACAACTC CAGTGGAAAA 
CAACCCTGGT TTTGTGCATG GAAAAGAGGG AGTGAGTTTC TGATGACACC CAACGTACCC 
TGCCGATCCT TGAAGAGGAA GAAAGAGAGC CAGGGGAGCG GGACCGCTGT GAGGAAACAC 
AAGCCTCCCC GGCTCAGTGA GATCAAGAGG AAGACGGACG AGCACCCTGA GCTTAAAGAA 
AAGCTGATGA ACAGGGTTGT GGATGAGTCC ATGAACCTTG AAGATGTCCC AGATTCTGTC 
AATCTTTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TAACGGAGTT CTCTATGGTG 
GACGGCATGT GGCAGGCGCA GGGCTATCCC CGGAATCGGT TACTGAGTGG CGACAGGTGG 
AGCCGGCTGA AGGCCTCCAG CTGCGAAAGC ATTCCTGAGG AAGACTCCGA GGCCAGGGCC 
TATGTCAACA GCCTGGGCTT AATGAGCACG CTGAGCCAGC CGGTCAGCAG GGCCAGCTCT 
GTCTCCAAGC AGTCGAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAC 
CAGATGGAAA ATGAAGGGAG AGGATTTGAG TTACTGCTGG ATTACTATGC TGGCAAGAAC 
GCCAGCAGCA TTCTGAACTC AGCCATGCAA CAGGCGTGCC GGAAAAGTGA CCACCTCAGT 
GTGAGGCCTA GCTGTCCCTC TAAGCAGTCC AGCACAGAGA GCATCACTGA GGAGTTCTAC 
AGGTACATGC TGAGGGACAT CGAAAGAGAC AGCAGAGAAA GTGCCTCCTC CAGACGGAGC 
AGCCAGGATT GGACAGCCGG CCTGCTGTCT CCTTCTCTGC GATCCCCAGT GTGCCACAGA 
CAGTCGTCCA TGCCAGACAG CAGATCCCCA TGCTCCAGGC TGACAGTGAA TGTGCCCATC 
AAAGCCAACT CTTTAGATGG CTTTGCTCAG AACTGCCCAC AAGATTTCCT AAGCGTGCAG 
CCGGTCAGTA GCGCGTCCTC ATCCGGTCTC TGCAAATCTG ACTCTTGCTT GTATCGGAGA 
GGTGGGACTG ACCACATCAC CAACATGTTA ATTCATGAAA CGTGGGCTAG CTCCATTGAG 
GCTCTCATGC GCAAGAACAA AATCATTGTG GATGATGCAG AGGAAGCTGA CACTGAGCCT 
GTTTCTGGTG GCTCTCCCTC GCAAGCAGAG AAGTGTGCAA ATAGATTAGC TGCGAGCAGG 
ATGTGCAGTG GGCCAACTCT GCTTGTTCAG GAGTCTCTCG ATTGCCCGAG GAAAGACTCT 
GTTACCGAAT GTAAACAGCC CCCAGTGTCA TCTTTGAGCA AAACTGCTTC TCTTACAAAC 
CACAGCCCTT TAGATTCTAA AAAAGAAACT TCCTCGTGCC AGGACCCTGT ACCAATAAAC 
CACAAAAGGC GATCACTTTG CTCGAGGGAA GTGCCTTTGA TTCAGATTGA AACAGATCAG 
AGAGAAGCCT GTGCTGGGGA ACCTGAACCC TTCCTTTCCA AAAGCAGCCT CCTAGAGGAA 
GCAGAAGGGC ATTCGAATGA CAAAAACATC CCAGATGTGG TGAGAGGTGG AGACACAGCC 
GTGAGCGCTT GTCAAATCCA TAGTGACAGC CTTGATACCA GAGATGTACC AGAGGCTGAA 
GCCTCCACAG AAGCCAGAGC CCCCGATGAG GCCCCCAACC CTCCAAGCAG CAGCGAGGAG 
AGCACAGGCA GCTGGACCCA GCTTGCCAAT GAGGAAGACA ACCCAGATGA CACAAGTAGC 
TTTCTCCAGC TCAGTGAGCG ATCCATGAGT GAATTAGTAG AAGAAAAGAA GATTCTTAAA 
GGACAGTCAG AAAGCACAGA GGCACCTGCA TCTGGACCGC CTACGGGAAC AGCCAGCCCC 
CAGAGGAGCC TGCTGGTGAT CAACTTTGAC CTGGAGCCAG AGTGTCCAGA TGCCGAGCTC 
CGAGCCACTC TGCAGTGGAT AGCTGCCTCT GAACTGGGGA TTCCCACCAT CTACTTTAAG 
AAATCTCAGG AAAACAGAAT TGAAAAGTTT CTAGATGTCG TGCAGCTGGT TCATCGGAAG 
TCCTGGAAAG TGGGTGATAT CTTCCATGCA GTTGTCCAGT ACTGCAAAAT GCATGAGGAG 
CAGAAGGATG GGAGACTGAG TCTCTTTGAC TGGCTCTTGG AACTGGGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712841.1
CDS	174..5264
Translation	MATPCSRYQAMWVVEPNSPTCLSAALPTRRHSQESSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQIGFVEDKSENCASVCFVNLDVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSIEEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPLIKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMDKDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAVSVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSILSGENSSRQPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCGMTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRPNTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSEILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSSSSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAGEDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLLPAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLKRKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLTEFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERDSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSLDGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEEADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLDSKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVRGGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLSERSMSELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCKMHEEQKDGRLSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006712778.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712778.4

ORF Insert Sequence:

ATGGCAACTC CCTGCTCTCG GTACCAAGCC ATGTGGGTAG TTGAACCAAA CTCACCTACC 
TGCCTCTCTG CTGCCTTGCC CACCAGGAGA CACTCCCAGG AATCCAGCAA CTTGGAGTCA 
TCACGGATGT ATGACGTTTT GGAACCGCAG CAGGGCAGAG GCTGTGGCAG CTCAGGAAGC 
GGCCCGGGGA ACTCCATCAC AGCCTGTAAG AAGGTTCTTC GCAGCAATAG CCTGCTGGAG 
TCAACAGACT ACTGGTTGCA GAATCAGAGG ATGCCCTGCC AAATTGGTTT TGTAGAAGAC 
AAGTCTGAAA ACTGTGCTTC TGTCTGCTTT GTGAATCTTG ATGTGAACAA GGATGAATGC 
AGCACAGAGC ACCTGCAACA GAAACTGGTC AACGTTTCAC CAGATCTTCC AAAACTTATC 
AGTTCCATGA ATGTCCAACA ACCAAAAGAA AATGAAATTG TTGTCCTAAG TGGGTTAGCC 
TCTGGAAATC TCCAGGCAGA TTTTGAAGTT TCACAGTGCC CTTGGCTGCC AGATATCTGC 
TTGGTCCAAT GTGCAAGAGG GAACAGACCA AACAGTACCA ACTGCATCAT CTTTGAAATC 
AACAAATTTC TGATTGGTCT GGAACTGGTG CAGGAGCGAC AGCTCCACCT GGAAACAAAC 
ATCTTGAAAC TGGAGGATGA CACGAACTGT TCCTTATCTT CAATCGAGGA AGACTTTCTC 
ACCGCTTCTG AGCACTTGGA GGAGGAAAGC GAGGTGGATG AATCTAGGAA CGATTATGAA 
AATATAAATG TCTCAGCCAA TGTTTTGGAA AGTAAACAGC TAAAGGGAGC CACCCAGGTG 
GAATGGAATT GCAACAAGGA AAAGTGGCTT TATGCTTTGG AAGACAAATA CATCAACAAA 
TATCCCACAC CATTGATTAA AACAGAACGA TCTCCAGAAA ACCTAACAAA GAACACAGCC 
TTGCAGAGTC TAGATCCCTC AGCCAAGCCA TCACAGTGGA AAAGAGAAGC TGTGGGGAAT 
GGGAGACAAG CCACACATTA TTATCATTCA GAAGCTTTTA AAGGTCAAAT GGAAAAATCA 
CAGGCACTGT ATATTCCAAA AGATGCTTAT TTCTCCATGA TGGATAAAGA TGTACCTTCT 
GCATGTGCTG TGGCAGAGCA GAGAAGCAAC CTAAACCCAG GAGACCATGA AGACACAAGA 
AACGCTCTCC CTCCTAGACA AGATGGAGAA GTCACCACTG GCAAGTATGC TACAAATTTA 
GCAGAATCCG TGCTGCAGGA TGCATTTATT AGATTATCTC AATCTCAGTC CACATTACCC 
CAGGAATCTG CAGTCAGTGT TTCTGTAGGA AGTTCTCTGC TTCCCAGTTG CTATTCCACA 
AAAGATACAG TGGTTTCTCG GTCATGGAAT GAGCTCCCCA AAATCGTCGT TGTTCAGAGT 
CCAGATGGCA GTGATGCTGC CCCACAGCCA GGCATCTCCT CCTGGCCTGA GATGGAAGTC 
TCTGTTGAAA CCTCAAGCAT CCTCTCTGGA GAGAACTCCA GCAGACAACC CCAGAGTGCT 
CTAGAAGTGG CGTTAGCTTG TGCAGCCACT GTGATTGGAA CTATTTCCAG TCCACAGGCC 
ACAGAAAGAC TCAAAATGGA GCAAGTGGTC TCGAACTTTC CCCCAGGGAG CAGTGGTGCA 
CTGCAAACTC AAGCACCCCA AGGACTCAAG GAACCTTCCA TCAATGAGTA CTCCTTTCCA 
TCTGCTTTGT GTGGCATGAC TCAGGTGGCC AGTGCCGTGG CTGTCTGTGG TCTGGGTGAA 
AGAGAAGAGG TGACATGCTC AGTGGCTCCA AGTGGTAGCC TCCCGCCTGC AGCTGAGGCT 
TCTGAAGCCA TGCCCCCACT TTGTGGTTTA GCAAGCATGG AGCTTGGCAA GGAAGCCATT 
GCCAAGGGAT TGCTCAAGGA GGCTGCTCTG GTTTTAACAA GGCCTAATAC CTACAGCAGC 
ATTGGAGACT TTCTGGACTC CATGAACAGG AGAATCATGG AAACTGCTTC AAAGTCTCAG 
ACCCTGTGCT CAGAAAATGT CGTCAGGAAT GAACTGGCAC ATACCCTGTC CAATGTTATC 
CTGAGGCATT CCATTGATGA AGTTCACCAC AAAAATATGA TAATCGACCC CAATGACAAC 
AGGCATTCAT CTGAAATTCT GGACACCTTA ATGGAAAGTA CAAATCAACT GCTTTTAGAT 
GTGATATGCT TCACGTTCAA GAAGATGAGT CATATTGTAC GGCTTGGTGA ATGTCCTGCT 
GTCCTTTCTA AGGAGACCAT CAGAAGGAGG GAGACAGAAC CAAGCTGCCA GCCATCTGAT 
CCGGGTGCTA GTCAAGCTTG GACAAAAGCC ACTGAATCCT CCAGCAGCTC TCCACTTAGC 
AATTCACACA ACACGAGTCT TGTCATCAAC AATCTTGTGG ATGGCATGTA TTCAAAACAA 
GACAAGGGTG GAGTGAGGCC AGGCCTCTTC AAGAACCCCA CGCTGCAGTC ACAATTATCA 
CGTAGTCACA GAGTGCCCGA TTCTTCAACT GCTACAACAT CCTCCAAGGA AATATATCTG 
AAAGGAATAG CAGGAGAGGA TACAAAAAGC CCTCATCACA GTGAGAATGA ATGCAGAGCC 
TCTTCCGAAG GACAAAGGTC CCCAACGGTC AGCCAGTCCA GAAGTGGTTC CCAGGAGGCT 
GAGGAGAGTA TCCACCCAAA CACCCAAGAA AAGTACAACT GTGCCACATC TCGCATCAAC 
GAAGTTCAAG TCAACCTGTC CTTGTTAGGG GATGACCTGC TGCTTCCTGC TCAATCCACG 
CTTCAAACAA AGCATCCAGA CATCTACTGC ATTACAGACT TTGCGGAAGA ATTAGCAGAC 
ACGGTCGTCT CCATGGCAAC TGAAATTGCA GCGATTTGCC TTGACAACTC CAGTGGAAAA 
CAACCCTGGT TTTGTGCATG GAAAAGAGGG AGTGAGTTTC TGATGACACC CAACGTACCC 
TGCCGATCCT TGAAGAGGAA GAAAGAGAGC CAGGGGAGCG GGACCGCTGT GAGGAAACAC 
AAGCCTCCCC GGCTCAGTGA GATCAAGAGG AAGACGGACG AGCACCCTGA GCTTAAAGAA 
AAGCTGATGA ACAGGGTTGT GGATGAGTCC ATGAACCTTG AAGATGTCCC AGATTCTGTC 
AATCTTTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TAACGGAGTT CTCTATGGTG 
GACGGCATGT GGCAGGCGCA GGGCTATCCC CGGAATCGGT TACTGAGTGG CGACAGGTGG 
AGCCGGCTGA AGGCCTCCAG CTGCGAAAGC ATTCCTGAGG AAGACTCCGA GGCCAGGGCC 
TATGTCAACA GCCTGGGCTT AATGAGCACG CTGAGCCAGC CGGTCAGCAG GGCCAGCTCT 
GTCTCCAAGC AGTCGAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAC 
CAGATGGAAA ATGAAGGGAG AGGATTTGAG TTACTGCTGG ATTACTATGC TGGCAAGAAC 
GCCAGCAGCA TTCTGAACTC AGCCATGCAA CAGGCGTGCC GGAAAAGTGA CCACCTCAGT 
GTGAGGCCTA GCTGTCCCTC TAAGCAGTCC AGCACAGAGA GCATCACTGA GGAGTTCTAC 
AGGTACATGC TGAGGGACAT CGAAAGAGAC AGCAGAGAAA GTGCCTCCTC CAGACGGAGC 
AGCCAGGATT GGACAGCCGG CCTGCTGTCT CCTTCTCTGC GATCCCCAGT GTGCCACAGA 
CAGTCGTCCA TGCCAGACAG CAGATCCCCA TGCTCCAGGC TGACAGTGAA TGTGCCCATC 
AAAGCCAACT CTTTAGATGG CTTTGCTCAG AACTGCCCAC AAGATTTCCT AAGCGTGCAG 
CCGGTCAGTA GCGCGTCCTC ATCCGGTCTC TGCAAATCTG ACTCTTGCTT GTATCGGAGA 
GGTGGGACTG ACCACATCAC CAACATGTTA ATTCATGAAA CGTGGGCTAG CTCCATTGAG 
GCTCTCATGC GCAAGAACAA AATCATTGTG GATGATGCAG AGGAAGCTGA CACTGAGCCT 
GTTTCTGGTG GCTCTCCCTC GCAAGCAGAG AAGTGTGCAA ATAGATTAGC TGCGAGCAGG 
ATGTGCAGTG GGCCAACTCT GCTTGTTCAG GAGTCTCTCG ATTGCCCGAG GAAAGACTCT 
GTTACCGAAT GTAAACAGCC CCCAGTGTCA TCTTTGAGCA AAACTGCTTC TCTTACAAAC 
CACAGCCCTT TAGATTCTAA AAAAGAAACT TCCTCGTGCC AGGACCCTGT ACCAATAAAC 
CACAAAAGGC GATCACTTTG CTCGAGGGAA GTGCCTTTGA TTCAGATTGA AACAGATCAG 
AGAGAAGCCT GTGCTGGGGA ACCTGAACCC TTCCTTTCCA AAAGCAGCCT CCTAGAGGAA 
GCAGAAGGGC ATTCGAATGA CAAAAACATC CCAGATGTGG TGAGAGGTGG AGACACAGCC 
GTGAGCGCTT GTCAAATCCA TAGTGACAGC CTTGATACCA GAGATGTACC AGAGGCTGAA 
GCCTCCACAG AAGCCAGAGC CCCCGATGAG GCCCCCAACC CTCCAAGCAG CAGCGAGGAG 
AGCACAGGCA GCTGGACCCA GCTTGCCAAT GAGGAAGACA ACCCAGATGA CACAAGTAGC 
TTTCTCCAGC TCAGTGAGCG ATCCATGAGT GAATTAGTAG AAGAAAAGAA GATTCTTAAA 
GGACAGTCAG AAAGCACAGA GGCACCTGCA TCTGGACCGC CTACGGGAAC AGCCAGCCCC 
CAGAGGAGCC TGCTGGTGAT CAACTTTGAC CTGGAGCCAG AGTGTCCAGA TGCCGAGCTC 
CGAGCCACTC TGCAGTGGAT AGCTGCCTCT GAACTGGGGA TTCCCACCAT CTACTTTAAG 
AAATCTCAGG AAAACAGAAT TGAAAAGTTT CTAGATGTCG TGCAGCTGGT TCATCGGAAG 
TCCTGGAAAG TGGGTGATAT CTTCCATGCA GTTGTCCAGT ACTGCAAAAT GCATGAGGAG 
CAGAAGGATG GGAGACTGAG TCTCTTTGAC TGGCTCTTGG AACTGGGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPHKAP ( XM_011511925.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510227.1 Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

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CloneID	OHu72743
Clone ID Related Accession (Same CDS sequence)	XM_011511925.2
Accession Version	XM_011511925.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511925.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTAG TTGAACCAAA CTCACCTACC TGCCTCTCTG CTGCCTTGCC CACCAGGAGA 
CACTCCCAGG AATCCAGCAA CTTGGAGTCA TCACGGATGT ATGACGTTTT GGAACCGCAG 
CAGGGCAGAG GCTGTGGCAG CTCAGGAAGC GGCCCGGGGA ACTCCATCAC AGCCTGTAAG 
AAGGTTCTTC GCAGCAATAG CCTGCTGGAG TCAACAGACT ACTGGTTGCA GAATCAGAGG 
ATGCCCTGCC AAATTGGTTT TGTAGAAGAC AAGTCTGAAA ACTGTGCTTC TGTCTGCTTT 
GTGAATCTTG ATGTGAACAA GGATGAATGC AGCACAGAGC ACCTGCAACA GAAACTGGTC 
AACGTTTCAC CAGATCTTCC AAAACTTATC AGTTCCATGA ATGTCCAACA ACCAAAAGAA 
AATGAAATTG TTGTCCTAAG TGGGTTAGCC TCTGGAAATC TCCAGGCAGA TTTTGAAGTT 
TCACAGTGCC CTTGGCTGCC AGATATCTGC TTGGTCCAAT GTGCAAGAGG GAACAGACCA 
AACAGTACCA ACTGCATCAT CTTTGAAATC AACAAATTTC TGATTGGTCT GGAACTGGTG 
CAGGAGCGAC AGCTCCACCT GGAAACAAAC ATCTTGAAAC TGGAGGATGA CACGAACTGT 
TCCTTATCTT CAATCGAGGA AGACTTTCTC ACCGCTTCTG AGCACTTGGA GGAGGAAAGC 
GAGGTGGATG AATCTAGGAA CGATTATGAA AATATAAATG TCTCAGCCAA TGTTTTGGAA 
AGTAAACAGC TAAAGGGAGC CACCCAGGTG GAATGGAATT GCAACAAGGA AAAGTGGCTT 
TATGCTTTGG AAGACAAATA CATCAACAAA TATCCCACAC CATTGATTAA AACAGAACGA 
TCTCCAGAAA ACCTAACAAA GAACACAGCC TTGCAGAGTC TAGATCCCTC AGCCAAGCCA 
TCACAGTGGA AAAGAGAAGC TGTGGGGAAT GGGAGACAAG CCACACATTA TTATCATTCA 
GAAGCTTTTA AAGGTCAAAT GGAAAAATCA CAGGCACTGT ATATTCCAAA AGATGCTTAT 
TTCTCCATGA TGGATAAAGA TGTACCTTCT GCATGTGCTG TGGCAGAGCA GAGAAGCAAC 
CTAAACCCAG GAGACCATGA AGACACAAGA AACGCTCTCC CTCCTAGACA AGATGGAGAA 
GTCACCACTG GCAAGTATGC TACAAATTTA GCAGAATCCG TGCTGCAGGA TGCATTTATT 
AGATTATCTC AATCTCAGTC CACATTACCC CAGGAATCTG CAGTCAGTGT TTCTGTAGGA 
AGTTCTCTGC TTCCCAGTTG CTATTCCACA AAAGATACAG TGGTTTCTCG GTCATGGAAT 
GAGCTCCCCA AAATCGTCGT TGTTCAGAGT CCAGATGGCA GTGATGCTGC CCCACAGCCA 
GGCATCTCCT CCTGGCCTGA GATGGAAGTC TCTGTTGAAA CCTCAAGCAT CCTCTCTGGA 
GAGAACTCCA GCAGACAACC CCAGAGTGCT CTAGAAGTGG CGTTAGCTTG TGCAGCCACT 
GTGATTGGAA CTATTTCCAG TCCACAGGCC ACAGAAAGAC TCAAAATGGA GCAAGTGGTC 
TCGAACTTTC CCCCAGGGAG CAGTGGTGCA CTGCAAACTC AAGCACCCCA AGGACTCAAG 
GAACCTTCCA TCAATGAGTA CTCCTTTCCA TCTGCTTTGT GTGGCATGAC TCAGGTGGCC 
AGTGCCGTGG CTGTCTGTGG TCTGGGTGAA AGAGAAGAGG TGACATGCTC AGTGGCTCCA 
AGTGGTAGCC TCCCGCCTGC AGCTGAGGCT TCTGAAGCCA TGCCCCCACT TTGTGGTTTA 
GCAAGCATGG AGCTTGGCAA GGAAGCCATT GCCAAGGGAT TGCTCAAGGA GGCTGCTCTG 
GTTTTAACAA GGCCTAATAC CTACAGCAGC ATTGGAGACT TTCTGGACTC CATGAACAGG 
AGAATCATGG AAACTGCTTC AAAGTCTCAG ACCCTGTGCT CAGAAAATGT CGTCAGGAAT 
GAACTGGCAC ATACCCTGTC CAATGTTATC CTGAGGCATT CCATTGATGA AGTTCACCAC 
AAAAATATGA TAATCGACCC CAATGACAAC AGGCATTCAT CTGAAATTCT GGACACCTTA 
ATGGAAAGTA CAAATCAACT GCTTTTAGAT GTGATATGCT TCACGTTCAA GAAGATGAGT 
CATATTGTAC GGCTTGGTGA ATGTCCTGCT GTCCTTTCTA AGGAGACCAT CAGAAGGAGG 
GAGACAGAAC CAAGCTGCCA GCCATCTGAT CCGGGTGCTA GTCAAGCTTG GACAAAAGCC 
ACTGAATCCT CCAGCAGCTC TCCACTTAGC AATTCACACA ACACGAGTCT TGTCATCAAC 
AATCTTGTGG ATGGCATGTA TTCAAAACAA GACAAGGGTG GAGTGAGGCC AGGCCTCTTC 
AAGAACCCCA CGCTGCAGTC ACAATTATCA CGTAGTCACA GAGTGCCCGA TTCTTCAACT 
GCTACAACAT CCTCCAAGGA AATATATCTG AAAGGAATAG CAGGAGAGGA TACAAAAAGC 
CCTCATCACA GTGAGAATGA ATGCAGAGCC TCTTCCGAAG GACAAAGGTC CCCAACGGTC 
AGCCAGTCCA GAAGTGGTTC CCAGGAGGCT GAGGAGAGTA TCCACCCAAA CACCCAAGAA 
AAGTACAACT GTGCCACATC TCGCATCAAC GAAGTTCAAG TCAACCTGTC CTTGTTAGGG 
GATGACCTGC TGCTTCCTGC TCAATCCACG CTTCAAACAA AGCATCCAGA CATCTACTGC 
ATTACAGACT TTGCGGAAGA ATTAGCAGAC ACGGTCGTCT CCATGGCAAC TGAAATTGCA 
GCGATTTGCC TTGACAACTC CAGTGGAAAA CAACCCTGGT TTTGTGCATG GAAAAGAGGG 
AGTGAGTTTC TGATGACACC CAACGTACCC TGCCGATCCT TGAAGAGGAA GAAAGAGAGC 
CAGGGGAGCG GGACCGCTGT GAGGAAACAC AAGCCTCCCC GGCTCAGTGA GATCAAGAGG 
AAGACGGACG AGCACCCTGA GCTTAAAGAA AAGCTGATGA ACAGGGTTGT GGATGAGTCC 
ATGAACCTTG AAGATGTCCC AGATTCTGTC AATCTTTTTG CCAATGAAGT GGCAGCCAAG 
ATCATGAACC TAACGGAGTT CTCTATGGTG GACGGCATGT GGCAGGCGCA GGGCTATCCC 
CGGAATCGGT TACTGAGTGG CGACAGGTGG AGCCGGCTGA AGGCCTCCAG CTGCGAAAGC 
ATTCCTGAGG AAGACTCCGA GGCCAGGGCC TATGTCAACA GCCTGGGCTT AATGAGCACG 
CTGAGCCAGC CGGTCAGCAG GGCCAGCTCT GTCTCCAAGC AGTCGAGCTG TGAGAGCATC 
ACCGATGAGT TTTCCAGGTT CATGGTGAAC CAGATGGAAA ATGAAGGGAG AGGATTTGAG 
TTACTGCTGG ATTACTATGC TGGCAAGAAC GCCAGCAGCA TTCTGAACTC AGCCATGCAA 
CAGGCGTGCC GGAAAAGTGA CCACCTCAGT GTGAGGCCTA GCTGTCCCTC TAAGCAGTCC 
AGCACAGAGA GCATCACTGA GGAGTTCTAC AGGTACATGC TGAGGGACAT CGAAAGAGAC 
AGCAGAGAAA GTGCCTCCTC CAGACGGAGC AGCCAGGATT GGACAGCCGG CCTGCTGTCT 
CCTTCTCTGC GATCCCCAGT GTGCCACAGA CAGTCGTCCA TGCCAGACAG CAGATCCCCA 
TGCTCCAGGC TGACAGTGAA TGTGCCCATC AAAGCCAACT CTTTAGATGG CTTTGCTCAG 
AACTGCCCAC AAGATTTCCT AAGCGTGCAG CCGGTCAGTA GCGCGTCCTC ATCCGGTCTC 
TGCAAATCTG ACTCTTGCTT GTATCGGAGA GGTGGGACTG ACCACATCAC CAACATGTTA 
ATTCATGAAA CGTGGGCTAG CTCCATTGAG GCTCTCATGC GCAAGAACAA AATCATTGTG 
GATGATGCAG AGGAAGCTGA CACTGAGCCT GTTTCTGGTG GCTCTCCCTC GCAAGCAGAG 
AAGTGTGCAA ATAGATTAGC TGCGAGCAGG ATGTGCAGTG GGCCAACTCT GCTTGTTCAG 
GAGTCTCTCG ATTGCCCGAG GAAAGACTCT GTTACCGAAT GTAAACAGCC CCCAGTGTCA 
TCTTTGAGCA AAACTGCTTC TCTTACAAAC CACAGCCCTT TAGATTCTAA AAAAGAAACT 
TCCTCGTGCC AGGACCCTGT ACCAATAAAC CACAAAAGGC GATCACTTTG CTCGAGGGAA 
GTGCCTTTGA TTCAGATTGA AACAGATCAG AGAGAAGCCT GTGCTGGGGA ACCTGAACCC 
TTCCTTTCCA AAAGCAGCCT CCTAGAGGAA GCAGAAGGGC ATTCGAATGA CAAAAACATC 
CCAGATGTGG TGAGAGGTGG AGACACAGCC GTGAGCGCTT GTCAAATCCA TAGTGACAGC 
CTTGATACCA GAGATGTACC AGAGGCTGAA GCCTCCACAG AAGCCAGAGC CCCCGATGAG 
GCCCCCAACC CTCCAAGCAG CAGCGAGGAG AGCACAGGCA GCTGGACCCA GCTTGCCAAT 
GAGGAAGACA ACCCAGATGA CACAAGTAGC TTTCTCCAGC TCAGTGAGCG ATCCATGAGC 
AATGGCAACA GTAGTGCCAC TAGCAGTCTT GGCATTATGG ATCTGGACAT TTATCAGGAA 
AGCATGCCAT CTTCTCCCAT GATTAATGAA TTAGTAGAAG AAAAGAAGAT TCTTAAAGGA 
CAGTCAGAAA GCACAGAGGC ACCTGCATCT GGACCGCCTA CGGGAACAGC CAGCCCCCAG 
AGGAGCCTGC TGGTGATCAA CTTTGACCTG GAGCCAGAGT GTCCAGATGC CGAGCTCCGA 
GCCACTCTGC AGTGGATAGC TGCCTCTGAA CTGGGGATTC CCACCATCTA CTTTAAGAAA 
TCTCAGGAAA ACAGAATTGA AAAGTTTCTA GATGTCGTGC AGCTGGTTCA TCGGAAGTCC 
TGGAAAGTGG GTGATATCTT CCATGCAGTT GTCCAGTACT GCAAAATGCA TGAGGAGCAG 
AAGGATGGGA GACTGAGTCT CTTTGACTGG CTCTTGGAAC TGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510227.1
CDS	554..5701
Translation	MWVVEPNSPTCLSAALPTRRHSQESSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQIGFVEDKSENCASVCFVNLDVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSIEEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPLIKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMDKDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAVSVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSILSGENSSRQPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCGMTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRPNTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSEILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSSSSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAGEDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLLPAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLKRKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLTEFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESITEEFYRYMLRDIERDSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSLDGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEEADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLDSKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVRGGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLSERSMSNGNSSATSSLGIMDLDIYQESMPSSPMINELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCKMHEEQKDGRLSLFDWLLELG

Target ORF information:

RefSeq Version	XM_011511925.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511925.2

ORF Insert Sequence:

ATGTGGGTAG TTGAACCAAA CTCACCTACC TGCCTCTCTG CTGCCTTGCC CACCAGGAGA 
CACTCCCAGG AATCCAGCAA CTTGGAGTCA TCACGGATGT ATGACGTTTT GGAACCGCAG 
CAGGGCAGAG GCTGTGGCAG CTCAGGAAGC GGCCCGGGGA ACTCCATCAC AGCCTGTAAG 
AAGGTTCTTC GCAGCAATAG CCTGCTGGAG TCAACAGACT ACTGGTTGCA GAATCAGAGG 
ATGCCCTGCC AAATTGGTTT TGTAGAAGAC AAGTCTGAAA ACTGTGCTTC TGTCTGCTTT 
GTGAATCTTG ATGTGAACAA GGATGAATGC AGCACAGAGC ACCTGCAACA GAAACTGGTC 
AACGTTTCAC CAGATCTTCC AAAACTTATC AGTTCCATGA ATGTCCAACA ACCAAAAGAA 
AATGAAATTG TTGTCCTAAG TGGGTTAGCC TCTGGAAATC TCCAGGCAGA TTTTGAAGTT 
TCACAGTGCC CTTGGCTGCC AGATATCTGC TTGGTCCAAT GTGCAAGAGG GAACAGACCA 
AACAGTACCA ACTGCATCAT CTTTGAAATC AACAAATTTC TGATTGGTCT GGAACTGGTG 
CAGGAGCGAC AGCTCCACCT GGAAACAAAC ATCTTGAAAC TGGAGGATGA CACGAACTGT 
TCCTTATCTT CAATCGAGGA AGACTTTCTC ACCGCTTCTG AGCACTTGGA GGAGGAAAGC 
GAGGTGGATG AATCTAGGAA CGATTATGAA AATATAAATG TCTCAGCCAA TGTTTTGGAA 
AGTAAACAGC TAAAGGGAGC CACCCAGGTG GAATGGAATT GCAACAAGGA AAAGTGGCTT 
TATGCTTTGG AAGACAAATA CATCAACAAA TATCCCACAC CATTGATTAA AACAGAACGA 
TCTCCAGAAA ACCTAACAAA GAACACAGCC TTGCAGAGTC TAGATCCCTC AGCCAAGCCA 
TCACAGTGGA AAAGAGAAGC TGTGGGGAAT GGGAGACAAG CCACACATTA TTATCATTCA 
GAAGCTTTTA AAGGTCAAAT GGAAAAATCA CAGGCACTGT ATATTCCAAA AGATGCTTAT 
TTCTCCATGA TGGATAAAGA TGTACCTTCT GCATGTGCTG TGGCAGAGCA GAGAAGCAAC 
CTAAACCCAG GAGACCATGA AGACACAAGA AACGCTCTCC CTCCTAGACA AGATGGAGAA 
GTCACCACTG GCAAGTATGC TACAAATTTA GCAGAATCCG TGCTGCAGGA TGCATTTATT 
AGATTATCTC AATCTCAGTC CACATTACCC CAGGAATCTG CAGTCAGTGT TTCTGTAGGA 
AGTTCTCTGC TTCCCAGTTG CTATTCCACA AAAGATACAG TGGTTTCTCG GTCATGGAAT 
GAGCTCCCCA AAATCGTCGT TGTTCAGAGT CCAGATGGCA GTGATGCTGC CCCACAGCCA 
GGCATCTCCT CCTGGCCTGA GATGGAAGTC TCTGTTGAAA CCTCAAGCAT CCTCTCTGGA 
GAGAACTCCA GCAGACAACC CCAGAGTGCT CTAGAAGTGG CGTTAGCTTG TGCAGCCACT 
GTGATTGGAA CTATTTCCAG TCCACAGGCC ACAGAAAGAC TCAAAATGGA GCAAGTGGTC 
TCGAACTTTC CCCCAGGGAG CAGTGGTGCA CTGCAAACTC AAGCACCCCA AGGACTCAAG 
GAACCTTCCA TCAATGAGTA CTCCTTTCCA TCTGCTTTGT GTGGCATGAC TCAGGTGGCC 
AGTGCCGTGG CTGTCTGTGG TCTGGGTGAA AGAGAAGAGG TGACATGCTC AGTGGCTCCA 
AGTGGTAGCC TCCCGCCTGC AGCTGAGGCT TCTGAAGCCA TGCCCCCACT TTGTGGTTTA 
GCAAGCATGG AGCTTGGCAA GGAAGCCATT GCCAAGGGAT TGCTCAAGGA GGCTGCTCTG 
GTTTTAACAA GGCCTAATAC CTACAGCAGC ATTGGAGACT TTCTGGACTC CATGAACAGG 
AGAATCATGG AAACTGCTTC AAAGTCTCAG ACCCTGTGCT CAGAAAATGT CGTCAGGAAT 
GAACTGGCAC ATACCCTGTC CAATGTTATC CTGAGGCATT CCATTGATGA AGTTCACCAC 
AAAAATATGA TAATCGACCC CAATGACAAC AGGCATTCAT CTGAAATTCT GGACACCTTA 
ATGGAAAGTA CAAATCAACT GCTTTTAGAT GTGATATGCT TCACGTTCAA GAAGATGAGT 
CATATTGTAC GGCTTGGTGA ATGTCCTGCT GTCCTTTCTA AGGAGACCAT CAGAAGGAGG 
GAGACAGAAC CAAGCTGCCA GCCATCTGAT CCGGGTGCTA GTCAAGCTTG GACAAAAGCC 
ACTGAATCCT CCAGCAGCTC TCCACTTAGC AATTCACACA ACACGAGTCT TGTCATCAAC 
AATCTTGTGG ATGGCATGTA TTCAAAACAA GACAAGGGTG GAGTGAGGCC AGGCCTCTTC 
AAGAACCCCA CGCTGCAGTC ACAATTATCA CGTAGTCACA GAGTGCCCGA TTCTTCAACT 
GCTACAACAT CCTCCAAGGA AATATATCTG AAAGGAATAG CAGGAGAGGA TACAAAAAGC 
CCTCATCACA GTGAGAATGA ATGCAGAGCC TCTTCCGAAG GACAAAGGTC CCCAACGGTC 
AGCCAGTCCA GAAGTGGTTC CCAGGAGGCT GAGGAGAGTA TCCACCCAAA CACCCAAGAA 
AAGTACAACT GTGCCACATC TCGCATCAAC GAAGTTCAAG TCAACCTGTC CTTGTTAGGG 
GATGACCTGC TGCTTCCTGC TCAATCCACG CTTCAAACAA AGCATCCAGA CATCTACTGC 
ATTACAGACT TTGCGGAAGA ATTAGCAGAC ACGGTCGTCT CCATGGCAAC TGAAATTGCA 
GCGATTTGCC TTGACAACTC CAGTGGAAAA CAACCCTGGT TTTGTGCATG GAAAAGAGGG 
AGTGAGTTTC TGATGACACC CAACGTACCC TGCCGATCCT TGAAGAGGAA GAAAGAGAGC 
CAGGGGAGCG GGACCGCTGT GAGGAAACAC AAGCCTCCCC GGCTCAGTGA GATCAAGAGG 
AAGACGGACG AGCACCCTGA GCTTAAAGAA AAGCTGATGA ACAGGGTTGT GGATGAGTCC 
ATGAACCTTG AAGATGTCCC AGATTCTGTC AATCTTTTTG CCAATGAAGT GGCAGCCAAG 
ATCATGAACC TAACGGAGTT CTCTATGGTG GACGGCATGT GGCAGGCGCA GGGCTATCCC 
CGGAATCGGT TACTGAGTGG CGACAGGTGG AGCCGGCTGA AGGCCTCCAG CTGCGAAAGC 
ATTCCTGAGG AAGACTCCGA GGCCAGGGCC TATGTCAACA GCCTGGGCTT AATGAGCACG 
CTGAGCCAGC CGGTCAGCAG GGCCAGCTCT GTCTCCAAGC AGTCGAGCTG TGAGAGCATC 
ACCGATGAGT TTTCCAGGTT CATGGTGAAC CAGATGGAAA ATGAAGGGAG AGGATTTGAG 
TTACTGCTGG ATTACTATGC TGGCAAGAAC GCCAGCAGCA TTCTGAACTC AGCCATGCAA 
CAGGCGTGCC GGAAAAGTGA CCACCTCAGT GTGAGGCCTA GCTGTCCCTC TAAGCAGTCC 
AGCACAGAGA GCATCACTGA GGAGTTCTAC AGGTACATGC TGAGGGACAT CGAAAGAGAC 
AGCAGAGAAA GTGCCTCCTC CAGACGGAGC AGCCAGGATT GGACAGCCGG CCTGCTGTCT 
CCTTCTCTGC GATCCCCAGT GTGCCACAGA CAGTCGTCCA TGCCAGACAG CAGATCCCCA 
TGCTCCAGGC TGACAGTGAA TGTGCCCATC AAAGCCAACT CTTTAGATGG CTTTGCTCAG 
AACTGCCCAC AAGATTTCCT AAGCGTGCAG CCGGTCAGTA GCGCGTCCTC ATCCGGTCTC 
TGCAAATCTG ACTCTTGCTT GTATCGGAGA GGTGGGACTG ACCACATCAC CAACATGTTA 
ATTCATGAAA CGTGGGCTAG CTCCATTGAG GCTCTCATGC GCAAGAACAA AATCATTGTG 
GATGATGCAG AGGAAGCTGA CACTGAGCCT GTTTCTGGTG GCTCTCCCTC GCAAGCAGAG 
AAGTGTGCAA ATAGATTAGC TGCGAGCAGG ATGTGCAGTG GGCCAACTCT GCTTGTTCAG 
GAGTCTCTCG ATTGCCCGAG GAAAGACTCT GTTACCGAAT GTAAACAGCC CCCAGTGTCA 
TCTTTGAGCA AAACTGCTTC TCTTACAAAC CACAGCCCTT TAGATTCTAA AAAAGAAACT 
TCCTCGTGCC AGGACCCTGT ACCAATAAAC CACAAAAGGC GATCACTTTG CTCGAGGGAA 
GTGCCTTTGA TTCAGATTGA AACAGATCAG AGAGAAGCCT GTGCTGGGGA ACCTGAACCC 
TTCCTTTCCA AAAGCAGCCT CCTAGAGGAA GCAGAAGGGC ATTCGAATGA CAAAAACATC 
CCAGATGTGG TGAGAGGTGG AGACACAGCC GTGAGCGCTT GTCAAATCCA TAGTGACAGC 
CTTGATACCA GAGATGTACC AGAGGCTGAA GCCTCCACAG AAGCCAGAGC CCCCGATGAG 
GCCCCCAACC CTCCAAGCAG CAGCGAGGAG AGCACAGGCA GCTGGACCCA GCTTGCCAAT 
GAGGAAGACA ACCCAGATGA CACAAGTAGC TTTCTCCAGC TCAGTGAGCG ATCCATGAGC 
AATGGCAACA GTAGTGCCAC TAGCAGTCTT GGCATTATGG ATCTGGACAT TTATCAGGAA 
AGCATGCCAT CTTCTCCCAT GATTAATGAA TTAGTAGAAG AAAAGAAGAT TCTTAAAGGA 
CAGTCAGAAA GCACAGAGGC ACCTGCATCT GGACCGCCTA CGGGAACAGC CAGCCCCCAG 
AGGAGCCTGC TGGTGATCAA CTTTGACCTG GAGCCAGAGT GTCCAGATGC CGAGCTCCGA 
GCCACTCTGC AGTGGATAGC TGCCTCTGAA CTGGGGATTC CCACCATCTA CTTTAAGAAA 
TCTCAGGAAA ACAGAATTGA AAAGTTTCTA GATGTCGTGC AGCTGGTTCA TCGGAAGTCC 
TGGAAAGTGG GTGATATCTT CCATGCAGTT GTCCAGTACT GCAAAATGCA TGAGGAGCAG 
AAGGATGGGA GACTGAGTCT CTTTGACTGG CTCTTGGAAC TGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic variants associated with disordered eating.
The International journal of eating disorders46(6)594-608(2013 Sep)
Wade TD,Gordon S,Medland S,Bulik CM,Heath AC,Montgomery GW,Martin NG

Genome wide analysis of acute myeloid leukemia reveal leukemia specific methylome and subtype specific hypomethylation of repeats.
PloS one7(3)e33213(2012)
Saied MH,Marzec J,Khalid S,Smith P,Down TA,Rakyan VK,Molloy G,Raghavan M,Debernardi S,Young BD

An entirely specific type I A-kinase anchoring protein that can sequester two molecules of protein kinase A at mitochondria.
Proceedings of the National Academy of Sciences of the United States of America108(48)E1227-35(2011 Nov)
Means CK,Lygren B,Langeberg LK,Jain A,Dixon RE,Vega AL,Gold MG,Petrosyan S,Taylor SS,Murphy AN,Ha T,Santana LF,Tasken K,Scott JD

Sphingosine kinase interacting protein is an A-kinase anchoring protein specific for type I cAMP-dependent protein kinase.
Chembiochem : a European journal of chemical biology11(7)963-71(2010 May)
Kovanich D,van der Heyden MA,Aye TT,van Veen TA,Heck AJ,Scholten A

Sphingosine kinase 1 is up-regulated during hypoxia in U87MG glioma cells. Role of hypoxia-inducible factors 1 and 2.
The Journal of biological chemistry283(6)3365-75(2008 Feb)
Anelli V,Gault CR,Cheng AB,Obeid LM

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SPHKAP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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