Sphkap gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006496578.3	XP_006496641.1	A-kinase anchor protein SPHKAP isoform X4
XM_006496576.3	XP_006496639.1	A-kinase anchor protein SPHKAP isoform X2
XM_006496577.3	XP_006496640.1	A-kinase anchor protein SPHKAP isoform X3
XM_006496576.4	XP_006496639.1	A-kinase anchor protein SPHKAP isoform X2
XM_006496575.3	XP_006496638.1	A-kinase anchor protein SPHKAP isoform X1
NM_172430.4	NP_766018.3	A-kinase anchor protein SPHKAP isoform 2
XM_006496575.4	XP_006496638.1	A-kinase anchor protein SPHKAP isoform X1
XM_006496577.4	XP_006496640.1	A-kinase anchor protein SPHKAP isoform X3
NM_172430.3	NP_766018.3	A-kinase anchor protein SPHKAP
NM_001356547.1	NP_001343476.1	A-kinase anchor protein SPHKAP isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu27826	XM_006496575.4 Latest version!	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27827	XM_006496576.4 Latest version!	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27828	XM_006496577.4 Latest version!	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu03435	NM_172430.4 Latest version!	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$972.30 $1389.00
OMu27829	NM_001356547.1 Latest version!	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu27829	XM_006496578.3 Latest version!	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu27827	XM_006496576.3	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu27826	XM_006496575.3	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu27828	XM_006496577.3	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu03435	NM_172430.3	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$972.30 $1389.00
Hide Old Accession Versions >>

Sphkap ( XM_006496575.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496638.1 Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu27826
Clone ID Related Accession (Same CDS sequence)	XM_006496575.3 , XM_006496575.4
Accession Version	XM_006496575.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496575.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCTGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC ATGAAGGTTC AGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAACCTG 
GCTGGCTCCA TCACGGCCTG TAAAAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGC TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGATGAGTCA 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAAGATGC CTGCATCACA 
GAGAACCTGC AGCAGAAACT GGTCAATGTT TCACCGGACC TTCCAAACCT CATCAGCTCC 
ATGAATGTCC AACAGCCGAA AGAAAATGAA ATTGTCCTCC TAAGTGGGTT AGCCTCTGGA 
AACCTCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGTGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT AGTGCAGGAG CGTCAGCTCC ACCTGGAGAC GAACGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCTCTG TCTTCAATTG AAGAAGATTT TCTTACAGCC 
TCCGAGCATT TGGAGGAAGA AATCGAGGTG GACGACTGCA GAAGTGGTTT GGAAAATACA 
AATGTGTCAG CTAATGTTTT GGAAAGTAAA AAGCCAAAGG AAACTACCCA GGAAGGATGG 
GATTACCACA AGGAAAAGTT GCATTGTGCT TTAGGGGAAA AACACATTAG AAAACACCGT 
ACGCCCTCCA CAAAAACAGA AGGGTCAAAG GAAAATACAG AAGAGAACAC GTCCTTGAAA 
AGTCTCAATC GTTTAGTCAG GCCATCACAC CTGAAAAGTG AAGTGGCGGG GAACAAGCAA 
CTGGCCACAA ACTACTCCTA CCCAGAAAAT ATCAAAGGGG AACTAGAAAC ATCTCAAATG 
CTGTTCATCC CTAGAGATGC CTATCTCTCC ATGGTTAAGA AAGATGTACT TTCTCCGTGT 
AGTGTACTCA GTGAGCAGGG AGGCAGCCAC AGAGACCATG ACGTTACTCC AAACCCTCTT 
CCTCCCGTTC AAAATGGAGA AGCCAGCACC GGAGAGTATG CCACAAACTT AGCAGAATCT 
GTAATGCAAG ATGCCTTCAT CCGATTGTCT CAGTCACAGC CCACACTCCC CCAGGAATCT 
GCAGTCAGTT TCTCTATGCG AAGTGCTCTC CTTCCCAGTG GCTGTTGCAC AAAAGATATG 
GTGGTCCCTC GTTCATGGAA CGAGCTACCC AAAATTGTCA TTGTGCAGAG CCCAGATGGC 
AGTGACACAG TCCCTGAGCC AAATGTCTCC TCCTGGCCTG ACATGGAATT TGTTGAAACC 
TCGGGCATCT TTTCTGCAGA CAGTTCCAGC AGACCCACTC AGAGTGCCCT AGAAGTTGCA 
TTGGCTTGTG CAGCCACTGT GATTGGAACC ATTTCCAGTC CACAGGCCAC AGAAAGATTC 
GCCATGGAGC AAGAATCCCT GGTATCTACC TATGCCCAGA GAGGCACTGG GGTACAGCAA 
ACTCAAGTGC CCCAAGCATT CATGGCACCT TCTACAACCG AGTATTCCTT CCCATCAGCT 
CTGTGTGGCA TGACACAAGT GGCAAGTGCT GTCGCTGTCT GTGGCTTATG TGAAAAAGAA 
GAGGCGACAT GTCCTGTAGC TCCAACTGAC CTCTTGCCTA CATCTGGGGC TTCTGAAGAA 
ATATCCTCCA TCGGAAGTTT AGTGATGGAG AGGAGCACAG AACTGGGGAA GGAAGCCATT 
GCAGAGGCTC TGCTCAGAGA GGCTACTCTG ATTTTAGCAA GGCCAGATGC TTACAGCAGC 
CTTGGTGAGC TCTTGGAATC TGTGAACCAG AGAATCATAG AAACCACTTC TAAAACCCAG 
ACCCTGTGCA CAGAAAGTGT TCAAAGGAAT GAACTGGCAC ACACCTTGTC CAATGTCATC 
CTCAAGCACT CCGTGGATGA ACTTCACCAG AAAACTACAA TGGCTCACCC CACTGATGAA 
AGGCATCCCT GTGGAACTCT GGACACCTTG ATGGAAAGTG TGAACCAGCT GCTGCACAAT 
GTGATCTGCT TCACATTCAA AAAGATGAAT CACATTGTAA CACTCAGTGA GCATCCCTCA 
TTTGATCAGG CTGCTGGTCA AGCCTGGGTA AAAGCCTTTG CATGCCCCAG CAGCCAGCCT 
CTTAGCAATG CACACGGTAC TGGCCTTGTC ATCAGGAATC TTGTAGAGGA TGCATCTCCT 
AAGTCAAACA AGGGCGGAGC AAGGCCAGAA CTGGTCAACA ACCCCAGGTT GCAATCTGAA 
TTCTCCTGCA GTCATAGAAT GTTTGATTCA ACTGCTAAGT CATTCCCCAA GGAAATATAT 
CTGAAAGGGA TTATGGGAGA GGATACCAGG AACCCTCATC ATACACTAAA TTATGACAGC 
AATGAACGAA GAGCCTCTAC AGACCTAGGA AAATTGACAA CAGCAAGCGA GGGTTGTAGT 
GGTTTCCAGG AAACTGAAGA CAGCATTGTT CCAAACACCC AAGAGAAATA CATCTGTGCC 
ACACCCCTAA ACAATGAAGC TCAAGTTAAC CTATCCTTAT TAGGTGATGA CCTGTCTGTT 
CCTGCTCAGT CTACTCTAGA GGCAAAGCAG TCCGAGGTCT ATGGCATCAC AGATTTTGCG 
GAAGAATTGG CAGAGACTGT TGTCTCCATG GCAACTGAAA TTGCAGCAAT CTGCCTTGAC 
AACTCCAATG GCAAACAGCC CTGGTTTTGT GCTTGGAAAA GAGGGAACGA GTTTTTAACG 
GCGCCTAACG GATCCTGCCG ATCCTTGAAG AGGAAGAAGG AAAACTCAAG CGCCGGAAGC 
ACCGTGAGGA AACACAAGCC ACCTCGCCTC AGTGAAATCA AGAGAAAAGC TGATGAGCAC 
CCAGAGCTGA AAGAGAAGCT GATGAACAGA GTCATGGATG AGTCCATGAA CCTCGAAGAC 
ATCCCTGATT CCGTCAGTAC CTTTGCCAAT GAAGTGGCAG CCAAGATCAT GAACCTCACA 
GAGTTTTCCA TGGTGGATGG GGTGTGGCAA GGCCAGAGTT GTTCCCGGAC TCGGCTTCTG 
GGTGGCGATA GGTGGAACCG GCTGAAGGCC TCAAGCTGTG AGAGCATTCC TGAGGAGGAC 
TCTGAAGCCA GGGTCTTTGT AAACAGCCTG GGTTTGATGA GTACCTTAAG CCAACCAGTT 
AGCAGGGCCA GCTCCGTCTC CAAACAGTCC AGCTGTGAGA GCATCACCGA TGAGTTTTCC 
AGGTTCATGG TGAAACAGAT GGAAAACGAA GGGAGAGGGT TTGAGTTGCT GCTGGATTAC 
TACGCAGGCA AAAATGCCAG CAGTATCATG AGCTCAGCGA TGCAGCAGGC ATGCCAGAAA 
AACGACCACC TCAACGTGAG ACCGAGCTGC CCCTCTAAGC AATCCAGTAC AGAGAGCATA 
ACAGAGGAGT TCTATAGGTA CATGTTAAGG GACATCGCGA AAGAAAGCAA AGATGGTGCC 
TCCTCCAGAC GTAGCAGCCA TGATTGGACC ACGGGCCTGC TGTCTCCTTC TACACGATCC 
CCTCTGTGTT ACAGACAGTC ATCTATGCCT GACAGCAGGT CGCCATGCTC CAGACTAACA 
GTAAATGCGC CTGTCAAAGC CAACTCCTTA GATGGCTTTG CCCAAAACTG CCCTCAAGAT 
TCTGTAAATG TACAGCCAGT CAGTAGGGCT TCCTCATCTG GCCTCTGCAA ATCAGATTCC 
TGCTTGTATC GCAGAAGTGG GACTGACCAG ATCACAAACA TGCTAATTCA TGAAACATGG 
GCCAGCTCCA TTGAGGCTCT CATGAGAAAG AACAAAATCA TTGCTGATGA TAGCGAGGCA 
GCTAATGCCA GTCCTGGCCC TGTTTCCAGT GGTTCTCCTT TGCAAGTAGA GAAGAATGCC 
AACAGATTAG CCACCAGCAA AGGACACAGG GGGCCAACTC TGCTTGTACA GGAATCTGTT 
GATTACCAGA GAAAAGATGC TGTTACTGAA GGCAATCATT CCCCAGTGTC ATCTCCAGGC 
AAAACAGCTC CTGTTAAAAA ACCCAGTGAT TTTGATCCTA GAAGAGAAAC CTCTGCATGC 
CACAATGCTG CTGGTCTCAA CAGCCCTAGG CGGTCACTCT GCTCAAGGGA TGTGCCTTTG 
ATTCAGATAG AGACAGATCA GAAAGAAGAG TGCATTGGAG AACCTGGACC TTTCCTTTCC 
CAAAGTGGCT CCCTAGAGGA AACAGAAGGC CACCAACCTG AAGAAACCAT CCCAGATGTG 
GCCAGAAATG AAGACACAGC TCCGAGCACC TGTCAGAGCT CTCGTGACAG CCTTGAGACC 
AGTGGGGAAG TAGAAGTGGA GGTCTTGAAA GAAGACATAC CCCGAGATGA GTCCCGGAAC 
CCTCCTAGCA GCAGCGAGGA GAGTACAGGC AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC 
ATCCCAGACG ACACAAGCAG CTTTCTGCAG CTCAGCGAGC GGTCCATGAG CAATGGCAAC 
AGTAGTGGCA CTAGCAGTCT TGGCATTATG GACCTGGACA TTTATCAGGA AAGCATACCG 
TCTTCTCCCA TGATTAATGA ATTAGTAGAA GAAAAGGAGA TTCTTAAAGA ACAATCAGAA 
AGCATAAAGG AACATGCCTC TGGACTGCCA GGGAGAGCTG CCAGCCCCCA GAGGAGCCTA 
CTGGTGATCA ACTTTGACCT GGAGCCAGAG TGTCCTGATG CTGAGCTTCG AGCCACTCTG 
CAGTGGATAG CTGCCTCCGA ACTGGGGATC CCAACAATCT ACTTTAAGAA ATCTCAGGAA 
AGCAGAATTG AAAAGTTTCT AGATGTTGTG AAGCTTGTTC AGCAGAAGTC CTGGAAGGTG 
GGAGATATAT TTCACGCGGT TGTCCAGTAC TGCAAACTGC ATGCAGAGCA GAAGGAGAGG 
ACTCCGAGTC TCTTTGACTG GCTTCTGGAA CTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496638.1
CDS	379..5517
Translation	MSTPGFLCKAMWVVEPNSATCLSAALPVKRHCQESSNVESPLMHEGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASGNLQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETNVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETTQEGWDYHKEKLHCALGEKHIRKHRTPSTKTEGSKENTEENTSLKSLNRLVRPSHLKSEVAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKDVLSPCSVLSEQGGSHRDHDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSMRSALLPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADSSSRPTQSALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQAFMAPSTTEYSFPSALCGMTQVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSIGSLVMERSTELGKEAIAEALLREATLILARPDAYSSLGELLESVNQRIIETTSKTQTLCTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCGTLDTLMESVNQLLHNVICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLVEDASPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTLNYDSNERRASTDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLLGDDLSVPAQSTLEAKQSEVYGITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCSRTRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEFYRYMLRDIAKESKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVNAPVKANSLDGFAQNCPQDSVNVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQVEKNANRLATSKGHRGPTLLVQESVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAGLNSPRRSLCSRDVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSSRDSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLSERSMSNGNSSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESIKEHASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006496575.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496575.4

ORF Insert Sequence:

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCTGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC ATGAAGGTTC AGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAACCTG 
GCTGGCTCCA TCACGGCCTG TAAAAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGC TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGATGAGTCA 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAAGATGC CTGCATCACA 
GAGAACCTGC AGCAGAAACT GGTCAATGTT TCACCGGACC TTCCAAACCT CATCAGCTCC 
ATGAATGTCC AACAGCCGAA AGAAAATGAA ATTGTCCTCC TAAGTGGGTT AGCCTCTGGA 
AACCTCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGTGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT AGTGCAGGAG CGTCAGCTCC ACCTGGAGAC GAACGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCTCTG TCTTCAATTG AAGAAGATTT TCTTACAGCC 
TCCGAGCATT TGGAGGAAGA AATCGAGGTG GACGACTGCA GAAGTGGTTT GGAAAATACA 
AATGTGTCAG CTAATGTTTT GGAAAGTAAA AAGCCAAAGG AAACTACCCA GGAAGGATGG 
GATTACCACA AGGAAAAGTT GCATTGTGCT TTAGGGGAAA AACACATTAG AAAACACCGT 
ACGCCCTCCA CAAAAACAGA AGGGTCAAAG GAAAATACAG AAGAGAACAC GTCCTTGAAA 
AGTCTCAATC GTTTAGTCAG GCCATCACAC CTGAAAAGTG AAGTGGCGGG GAACAAGCAA 
CTGGCCACAA ACTACTCCTA CCCAGAAAAT ATCAAAGGGG AACTAGAAAC ATCTCAAATG 
CTGTTCATCC CTAGAGATGC CTATCTCTCC ATGGTTAAGA AAGATGTACT TTCTCCGTGT 
AGTGTACTCA GTGAGCAGGG AGGCAGCCAC AGAGACCATG ACGTTACTCC AAACCCTCTT 
CCTCCCGTTC AAAATGGAGA AGCCAGCACC GGAGAGTATG CCACAAACTT AGCAGAATCT 
GTAATGCAAG ATGCCTTCAT CCGATTGTCT CAGTCACAGC CCACACTCCC CCAGGAATCT 
GCAGTCAGTT TCTCTATGCG AAGTGCTCTC CTTCCCAGTG GCTGTTGCAC AAAAGATATG 
GTGGTCCCTC GTTCATGGAA CGAGCTACCC AAAATTGTCA TTGTGCAGAG CCCAGATGGC 
AGTGACACAG TCCCTGAGCC AAATGTCTCC TCCTGGCCTG ACATGGAATT TGTTGAAACC 
TCGGGCATCT TTTCTGCAGA CAGTTCCAGC AGACCCACTC AGAGTGCCCT AGAAGTTGCA 
TTGGCTTGTG CAGCCACTGT GATTGGAACC ATTTCCAGTC CACAGGCCAC AGAAAGATTC 
GCCATGGAGC AAGAATCCCT GGTATCTACC TATGCCCAGA GAGGCACTGG GGTACAGCAA 
ACTCAAGTGC CCCAAGCATT CATGGCACCT TCTACAACCG AGTATTCCTT CCCATCAGCT 
CTGTGTGGCA TGACACAAGT GGCAAGTGCT GTCGCTGTCT GTGGCTTATG TGAAAAAGAA 
GAGGCGACAT GTCCTGTAGC TCCAACTGAC CTCTTGCCTA CATCTGGGGC TTCTGAAGAA 
ATATCCTCCA TCGGAAGTTT AGTGATGGAG AGGAGCACAG AACTGGGGAA GGAAGCCATT 
GCAGAGGCTC TGCTCAGAGA GGCTACTCTG ATTTTAGCAA GGCCAGATGC TTACAGCAGC 
CTTGGTGAGC TCTTGGAATC TGTGAACCAG AGAATCATAG AAACCACTTC TAAAACCCAG 
ACCCTGTGCA CAGAAAGTGT TCAAAGGAAT GAACTGGCAC ACACCTTGTC CAATGTCATC 
CTCAAGCACT CCGTGGATGA ACTTCACCAG AAAACTACAA TGGCTCACCC CACTGATGAA 
AGGCATCCCT GTGGAACTCT GGACACCTTG ATGGAAAGTG TGAACCAGCT GCTGCACAAT 
GTGATCTGCT TCACATTCAA AAAGATGAAT CACATTGTAA CACTCAGTGA GCATCCCTCA 
TTTGATCAGG CTGCTGGTCA AGCCTGGGTA AAAGCCTTTG CATGCCCCAG CAGCCAGCCT 
CTTAGCAATG CACACGGTAC TGGCCTTGTC ATCAGGAATC TTGTAGAGGA TGCATCTCCT 
AAGTCAAACA AGGGCGGAGC AAGGCCAGAA CTGGTCAACA ACCCCAGGTT GCAATCTGAA 
TTCTCCTGCA GTCATAGAAT GTTTGATTCA ACTGCTAAGT CATTCCCCAA GGAAATATAT 
CTGAAAGGGA TTATGGGAGA GGATACCAGG AACCCTCATC ATACACTAAA TTATGACAGC 
AATGAACGAA GAGCCTCTAC AGACCTAGGA AAATTGACAA CAGCAAGCGA GGGTTGTAGT 
GGTTTCCAGG AAACTGAAGA CAGCATTGTT CCAAACACCC AAGAGAAATA CATCTGTGCC 
ACACCCCTAA ACAATGAAGC TCAAGTTAAC CTATCCTTAT TAGGTGATGA CCTGTCTGTT 
CCTGCTCAGT CTACTCTAGA GGCAAAGCAG TCCGAGGTCT ATGGCATCAC AGATTTTGCG 
GAAGAATTGG CAGAGACTGT TGTCTCCATG GCAACTGAAA TTGCAGCAAT CTGCCTTGAC 
AACTCCAATG GCAAACAGCC CTGGTTTTGT GCTTGGAAAA GAGGGAACGA GTTTTTAACG 
GCGCCTAACG GATCCTGCCG ATCCTTGAAG AGGAAGAAGG AAAACTCAAG CGCCGGAAGC 
ACCGTGAGGA AACACAAGCC ACCTCGCCTC AGTGAAATCA AGAGAAAAGC TGATGAGCAC 
CCAGAGCTGA AAGAGAAGCT GATGAACAGA GTCATGGATG AGTCCATGAA CCTCGAAGAC 
ATCCCTGATT CCGTCAGTAC CTTTGCCAAT GAAGTGGCAG CCAAGATCAT GAACCTCACA 
GAGTTTTCCA TGGTGGATGG GGTGTGGCAA GGCCAGAGTT GTTCCCGGAC TCGGCTTCTG 
GGTGGCGATA GGTGGAACCG GCTGAAGGCC TCAAGCTGTG AGAGCATTCC TGAGGAGGAC 
TCTGAAGCCA GGGTCTTTGT AAACAGCCTG GGTTTGATGA GTACCTTAAG CCAACCAGTT 
AGCAGGGCCA GCTCCGTCTC CAAACAGTCC AGCTGTGAGA GCATCACCGA TGAGTTTTCC 
AGGTTCATGG TGAAACAGAT GGAAAACGAA GGGAGAGGGT TTGAGTTGCT GCTGGATTAC 
TACGCAGGCA AAAATGCCAG CAGTATCATG AGCTCAGCGA TGCAGCAGGC ATGCCAGAAA 
AACGACCACC TCAACGTGAG ACCGAGCTGC CCCTCTAAGC AATCCAGTAC AGAGAGCATA 
ACAGAGGAGT TCTATAGGTA CATGTTAAGG GACATCGCGA AAGAAAGCAA AGATGGTGCC 
TCCTCCAGAC GTAGCAGCCA TGATTGGACC ACGGGCCTGC TGTCTCCTTC TACACGATCC 
CCTCTGTGTT ACAGACAGTC ATCTATGCCT GACAGCAGGT CGCCATGCTC CAGACTAACA 
GTAAATGCGC CTGTCAAAGC CAACTCCTTA GATGGCTTTG CCCAAAACTG CCCTCAAGAT 
TCTGTAAATG TACAGCCAGT CAGTAGGGCT TCCTCATCTG GCCTCTGCAA ATCAGATTCC 
TGCTTGTATC GCAGAAGTGG GACTGACCAG ATCACAAACA TGCTAATTCA TGAAACATGG 
GCCAGCTCCA TTGAGGCTCT CATGAGAAAG AACAAAATCA TTGCTGATGA TAGCGAGGCA 
GCTAATGCCA GTCCTGGCCC TGTTTCCAGT GGTTCTCCTT TGCAAGTAGA GAAGAATGCC 
AACAGATTAG CCACCAGCAA AGGACACAGG GGGCCAACTC TGCTTGTACA GGAATCTGTT 
GATTACCAGA GAAAAGATGC TGTTACTGAA GGCAATCATT CCCCAGTGTC ATCTCCAGGC 
AAAACAGCTC CTGTTAAAAA ACCCAGTGAT TTTGATCCTA GAAGAGAAAC CTCTGCATGC 
CACAATGCTG CTGGTCTCAA CAGCCCTAGG CGGTCACTCT GCTCAAGGGA TGTGCCTTTG 
ATTCAGATAG AGACAGATCA GAAAGAAGAG TGCATTGGAG AACCTGGACC TTTCCTTTCC 
CAAAGTGGCT CCCTAGAGGA AACAGAAGGC CACCAACCTG AAGAAACCAT CCCAGATGTG 
GCCAGAAATG AAGACACAGC TCCGAGCACC TGTCAGAGCT CTCGTGACAG CCTTGAGACC 
AGTGGGGAAG TAGAAGTGGA GGTCTTGAAA GAAGACATAC CCCGAGATGA GTCCCGGAAC 
CCTCCTAGCA GCAGCGAGGA GAGTACAGGC AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC 
ATCCCAGACG ACACAAGCAG CTTTCTGCAG CTCAGCGAGC GGTCCATGAG CAATGGCAAC 
AGTAGTGGCA CTAGCAGTCT TGGCATTATG GACCTGGACA TTTATCAGGA AAGCATACCG 
TCTTCTCCCA TGATTAATGA ATTAGTAGAA GAAAAGGAGA TTCTTAAAGA ACAATCAGAA 
AGCATAAAGG AACATGCCTC TGGACTGCCA GGGAGAGCTG CCAGCCCCCA GAGGAGCCTA 
CTGGTGATCA ACTTTGACCT GGAGCCAGAG TGTCCTGATG CTGAGCTTCG AGCCACTCTG 
CAGTGGATAG CTGCCTCCGA ACTGGGGATC CCAACAATCT ACTTTAAGAA ATCTCAGGAA 
AGCAGAATTG AAAAGTTTCT AGATGTTGTG AAGCTTGTTC AGCAGAAGTC CTGGAAGGTG 
GGAGATATAT TTCACGCGGT TGTCCAGTAC TGCAAACTGC ATGCAGAGCA GAAGGAGAGG 
ACTCCGAGTC TCTTTGACTG GCTTCTGGAA CTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( XM_006496576.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496639.1 Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X2, mRNA.

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CloneID	OMu27827
Clone ID Related Accession (Same CDS sequence)	XM_006496576.3 , XM_006496576.4
Accession Version	XM_006496576.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496576.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTAG TTGAACCAAA CTCTGCTACC TGCCTTTCTG CTGCCCTGCC GGTCAAGAGA 
CACTGCCAAG AATCCAGCAA CGTGGAATCA CCACTGATGC ATGAAGGTTC AGAGCCACAG 
CAGATCACCA GCAGTGCAGC AGGCAACCTG GCTGGCTCCA TCACGGCCTG TAAAAAGGTT 
CTTCGAAGCA ATAGCTTGCT GGAGTCCACA GACTACTGGC TGCAGAACCA GAGGACGCCT 
TGCCAGATTG GGTTTGTGGA AGATGAGTCA GAAAACTGTG CTTCTGTCTG CTTTGTGAAC 
CTGGATGTCA ACAAAGATGC CTGCATCACA GAGAACCTGC AGCAGAAACT GGTCAATGTT 
TCACCGGACC TTCCAAACCT CATCAGCTCC ATGAATGTCC AACAGCCGAA AGAAAATGAA 
ATTGTCCTCC TAAGTGGGTT AGCCTCTGGA AACCTCCAGG CAGATTTTGA TGTTTCACAG 
TGCCCTTGGC TACCAGATAT CTGCTTGGTC CAGTGTGCAA GAGGGAACAG ACCAAACAGT 
ACCAACTGCA TCATCTTTGA GATCAACAAG TTCTTAATTG GTCTGGAAGT AGTGCAGGAG 
CGTCAGCTCC ACCTGGAGAC GAACGTGTTG AAGCTGGAGG ATGATACAAA CTGTTCTCTG 
TCTTCAATTG AAGAAGATTT TCTTACAGCC TCCGAGCATT TGGAGGAAGA AATCGAGGTG 
GACGACTGCA GAAGTGGTTT GGAAAATACA AATGTGTCAG CTAATGTTTT GGAAAGTAAA 
AAGCCAAAGG AAACTACCCA GGAAGGATGG GATTACCACA AGGAAAAGTT GCATTGTGCT 
TTAGGGGAAA AACACATTAG AAAACACCGT ACGCCCTCCA CAAAAACAGA AGGGTCAAAG 
GAAAATACAG AAGAGAACAC GTCCTTGAAA AGTCTCAATC GTTTAGTCAG GCCATCACAC 
CTGAAAAGTG AAGTGGCGGG GAACAAGCAA CTGGCCACAA ACTACTCCTA CCCAGAAAAT 
ATCAAAGGGG AACTAGAAAC ATCTCAAATG CTGTTCATCC CTAGAGATGC CTATCTCTCC 
ATGGTTAAGA AAGATGTACT TTCTCCGTGT AGTGTACTCA GTGAGCAGGG AGGCAGCCAC 
AGAGACCATG ACGTTACTCC AAACCCTCTT CCTCCCGTTC AAAATGGAGA AGCCAGCACC 
GGAGAGTATG CCACAAACTT AGCAGAATCT GTAATGCAAG ATGCCTTCAT CCGATTGTCT 
CAGTCACAGC CCACACTCCC CCAGGAATCT GCAGTCAGTT TCTCTATGCG AAGTGCTCTC 
CTTCCCAGTG GCTGTTGCAC AAAAGATATG GTGGTCCCTC GTTCATGGAA CGAGCTACCC 
AAAATTGTCA TTGTGCAGAG CCCAGATGGC AGTGACACAG TCCCTGAGCC AAATGTCTCC 
TCCTGGCCTG ACATGGAATT TGTTGAAACC TCGGGCATCT TTTCTGCAGA CAGTTCCAGC 
AGACCCACTC AGAGTGCCCT AGAAGTTGCA TTGGCTTGTG CAGCCACTGT GATTGGAACC 
ATTTCCAGTC CACAGGCCAC AGAAAGATTC GCCATGGAGC AAGAATCCCT GGTATCTACC 
TATGCCCAGA GAGGCACTGG GGTACAGCAA ACTCAAGTGC CCCAAGCATT CATGGCACCT 
TCTACAACCG AGTATTCCTT CCCATCAGCT CTGTGTGGCA TGACACAAGT GGCAAGTGCT 
GTCGCTGTCT GTGGCTTATG TGAAAAAGAA GAGGCGACAT GTCCTGTAGC TCCAACTGAC 
CTCTTGCCTA CATCTGGGGC TTCTGAAGAA ATATCCTCCA TCGGAAGTTT AGTGATGGAG 
AGGAGCACAG AACTGGGGAA GGAAGCCATT GCAGAGGCTC TGCTCAGAGA GGCTACTCTG 
ATTTTAGCAA GGCCAGATGC TTACAGCAGC CTTGGTGAGC TCTTGGAATC TGTGAACCAG 
AGAATCATAG AAACCACTTC TAAAACCCAG ACCCTGTGCA CAGAAAGTGT TCAAAGGAAT 
GAACTGGCAC ACACCTTGTC CAATGTCATC CTCAAGCACT CCGTGGATGA ACTTCACCAG 
AAAACTACAA TGGCTCACCC CACTGATGAA AGGCATCCCT GTGGAACTCT GGACACCTTG 
ATGGAAAGTG TGAACCAGCT GCTGCACAAT GTGATCTGCT TCACATTCAA AAAGATGAAT 
CACATTGTAA CACTCAGTGA GCATCCCTCA TTTGATCAGG CTGCTGGTCA AGCCTGGGTA 
AAAGCCTTTG CATGCCCCAG CAGCCAGCCT CTTAGCAATG CACACGGTAC TGGCCTTGTC 
ATCAGGAATC TTGTAGAGGA TGCATCTCCT AAGTCAAACA AGGGCGGAGC AAGGCCAGAA 
CTGGTCAACA ACCCCAGGTT GCAATCTGAA TTCTCCTGCA GTCATAGAAT GTTTGATTCA 
ACTGCTAAGT CATTCCCCAA GGAAATATAT CTGAAAGGGA TTATGGGAGA GGATACCAGG 
AACCCTCATC ATACACTAAA TTATGACAGC AATGAACGAA GAGCCTCTAC AGACCTAGGA 
AAATTGACAA CAGCAAGCGA GGGTTGTAGT GGTTTCCAGG AAACTGAAGA CAGCATTGTT 
CCAAACACCC AAGAGAAATA CATCTGTGCC ACACCCCTAA ACAATGAAGC TCAAGTTAAC 
CTATCCTTAT TAGGTGATGA CCTGTCTGTT CCTGCTCAGT CTACTCTAGA GGCAAAGCAG 
TCCGAGGTCT ATGGCATCAC AGATTTTGCG GAAGAATTGG CAGAGACTGT TGTCTCCATG 
GCAACTGAAA TTGCAGCAAT CTGCCTTGAC AACTCCAATG GCAAACAGCC CTGGTTTTGT 
GCTTGGAAAA GAGGGAACGA GTTTTTAACG GCGCCTAACG GATCCTGCCG ATCCTTGAAG 
AGGAAGAAGG AAAACTCAAG CGCCGGAAGC ACCGTGAGGA AACACAAGCC ACCTCGCCTC 
AGTGAAATCA AGAGAAAAGC TGATGAGCAC CCAGAGCTGA AAGAGAAGCT GATGAACAGA 
GTCATGGATG AGTCCATGAA CCTCGAAGAC ATCCCTGATT CCGTCAGTAC CTTTGCCAAT 
GAAGTGGCAG CCAAGATCAT GAACCTCACA GAGTTTTCCA TGGTGGATGG GGTGTGGCAA 
GGCCAGAGTT GTTCCCGGAC TCGGCTTCTG GGTGGCGATA GGTGGAACCG GCTGAAGGCC 
TCAAGCTGTG AGAGCATTCC TGAGGAGGAC TCTGAAGCCA GGGTCTTTGT AAACAGCCTG 
GGTTTGATGA GTACCTTAAG CCAACCAGTT AGCAGGGCCA GCTCCGTCTC CAAACAGTCC 
AGCTGTGAGA GCATCACCGA TGAGTTTTCC AGGTTCATGG TGAAACAGAT GGAAAACGAA 
GGGAGAGGGT TTGAGTTGCT GCTGGATTAC TACGCAGGCA AAAATGCCAG CAGTATCATG 
AGCTCAGCGA TGCAGCAGGC ATGCCAGAAA AACGACCACC TCAACGTGAG ACCGAGCTGC 
CCCTCTAAGC AATCCAGTAC AGAGAGCATA ACAGAGGAGT TCTATAGGTA CATGTTAAGG 
GACATCGCGA AAGAAAGCAA AGATGGTGCC TCCTCCAGAC GTAGCAGCCA TGATTGGACC 
ACGGGCCTGC TGTCTCCTTC TACACGATCC CCTCTGTGTT ACAGACAGTC ATCTATGCCT 
GACAGCAGGT CGCCATGCTC CAGACTAACA GTAAATGCGC CTGTCAAAGC CAACTCCTTA 
GATGGCTTTG CCCAAAACTG CCCTCAAGAT TCTGTAAATG TACAGCCAGT CAGTAGGGCT 
TCCTCATCTG GCCTCTGCAA ATCAGATTCC TGCTTGTATC GCAGAAGTGG GACTGACCAG 
ATCACAAACA TGCTAATTCA TGAAACATGG GCCAGCTCCA TTGAGGCTCT CATGAGAAAG 
AACAAAATCA TTGCTGATGA TAGCGAGGCA GCTAATGCCA GTCCTGGCCC TGTTTCCAGT 
GGTTCTCCTT TGCAAGTAGA GAAGAATGCC AACAGATTAG CCACCAGCAA AGGACACAGG 
GGGCCAACTC TGCTTGTACA GGAATCTGTT GATTACCAGA GAAAAGATGC TGTTACTGAA 
GGCAATCATT CCCCAGTGTC ATCTCCAGGC AAAACAGCTC CTGTTAAAAA ACCCAGTGAT 
TTTGATCCTA GAAGAGAAAC CTCTGCATGC CACAATGCTG CTGGTCTCAA CAGCCCTAGG 
CGGTCACTCT GCTCAAGGGA TGTGCCTTTG ATTCAGATAG AGACAGATCA GAAAGAAGAG 
TGCATTGGAG AACCTGGACC TTTCCTTTCC CAAAGTGGCT CCCTAGAGGA AACAGAAGGC 
CACCAACCTG AAGAAACCAT CCCAGATGTG GCCAGAAATG AAGACACAGC TCCGAGCACC 
TGTCAGAGCT CTCGTGACAG CCTTGAGACC AGTGGGGAAG TAGAAGTGGA GGTCTTGAAA 
GAAGACATAC CCCGAGATGA GTCCCGGAAC CCTCCTAGCA GCAGCGAGGA GAGTACAGGC 
AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC ATCCCAGACG ACACAAGCAG CTTTCTGCAG 
CTCAGCGAGC GGTCCATGAG CAATGGCAAC AGTAGTGGCA CTAGCAGTCT TGGCATTATG 
GACCTGGACA TTTATCAGGA AAGCATACCG TCTTCTCCCA TGATTAATGA ATTAGTAGAA 
GAAAAGGAGA TTCTTAAAGA ACAATCAGAA AGCATAAAGG AACATGCCTC TGGACTGCCA 
GGGAGAGCTG CCAGCCCCCA GAGGAGCCTA CTGGTGATCA ACTTTGACCT GGAGCCAGAG 
TGTCCTGATG CTGAGCTTCG AGCCACTCTG CAGTGGATAG CTGCCTCCGA ACTGGGGATC 
CCAACAATCT ACTTTAAGAA ATCTCAGGAA AGCAGAATTG AAAAGTTTCT AGATGTTGTG 
AAGCTTGTTC AGCAGAAGTC CTGGAAGGTG GGAGATATAT TTCACGCGGT TGTCCAGTAC 
TGCAAACTGC ATGCAGAGCA GAAGGAGAGG ACTCCGAGTC TCTTTGACTG GCTTCTGGAA 
CTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496639.1
CDS	390..5498
Translation	MWVVEPNSATCLSAALPVKRHCQESSNVESPLMHEGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASGNLQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETNVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETTQEGWDYHKEKLHCALGEKHIRKHRTPSTKTEGSKENTEENTSLKSLNRLVRPSHLKSEVAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKDVLSPCSVLSEQGGSHRDHDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSMRSALLPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADSSSRPTQSALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQAFMAPSTTEYSFPSALCGMTQVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSIGSLVMERSTELGKEAIAEALLREATLILARPDAYSSLGELLESVNQRIIETTSKTQTLCTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCGTLDTLMESVNQLLHNVICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLVEDASPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTLNYDSNERRASTDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLLGDDLSVPAQSTLEAKQSEVYGITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCSRTRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEFYRYMLRDIAKESKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVNAPVKANSLDGFAQNCPQDSVNVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQVEKNANRLATSKGHRGPTLLVQESVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAGLNSPRRSLCSRDVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSSRDSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLSERSMSNGNSSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESIKEHASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006496576.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496576.4

ORF Insert Sequence:

ATGTGGGTAG TTGAACCAAA CTCTGCTACC TGCCTTTCTG CTGCCCTGCC GGTCAAGAGA 
CACTGCCAAG AATCCAGCAA CGTGGAATCA CCACTGATGC ATGAAGGTTC AGAGCCACAG 
CAGATCACCA GCAGTGCAGC AGGCAACCTG GCTGGCTCCA TCACGGCCTG TAAAAAGGTT 
CTTCGAAGCA ATAGCTTGCT GGAGTCCACA GACTACTGGC TGCAGAACCA GAGGACGCCT 
TGCCAGATTG GGTTTGTGGA AGATGAGTCA GAAAACTGTG CTTCTGTCTG CTTTGTGAAC 
CTGGATGTCA ACAAAGATGC CTGCATCACA GAGAACCTGC AGCAGAAACT GGTCAATGTT 
TCACCGGACC TTCCAAACCT CATCAGCTCC ATGAATGTCC AACAGCCGAA AGAAAATGAA 
ATTGTCCTCC TAAGTGGGTT AGCCTCTGGA AACCTCCAGG CAGATTTTGA TGTTTCACAG 
TGCCCTTGGC TACCAGATAT CTGCTTGGTC CAGTGTGCAA GAGGGAACAG ACCAAACAGT 
ACCAACTGCA TCATCTTTGA GATCAACAAG TTCTTAATTG GTCTGGAAGT AGTGCAGGAG 
CGTCAGCTCC ACCTGGAGAC GAACGTGTTG AAGCTGGAGG ATGATACAAA CTGTTCTCTG 
TCTTCAATTG AAGAAGATTT TCTTACAGCC TCCGAGCATT TGGAGGAAGA AATCGAGGTG 
GACGACTGCA GAAGTGGTTT GGAAAATACA AATGTGTCAG CTAATGTTTT GGAAAGTAAA 
AAGCCAAAGG AAACTACCCA GGAAGGATGG GATTACCACA AGGAAAAGTT GCATTGTGCT 
TTAGGGGAAA AACACATTAG AAAACACCGT ACGCCCTCCA CAAAAACAGA AGGGTCAAAG 
GAAAATACAG AAGAGAACAC GTCCTTGAAA AGTCTCAATC GTTTAGTCAG GCCATCACAC 
CTGAAAAGTG AAGTGGCGGG GAACAAGCAA CTGGCCACAA ACTACTCCTA CCCAGAAAAT 
ATCAAAGGGG AACTAGAAAC ATCTCAAATG CTGTTCATCC CTAGAGATGC CTATCTCTCC 
ATGGTTAAGA AAGATGTACT TTCTCCGTGT AGTGTACTCA GTGAGCAGGG AGGCAGCCAC 
AGAGACCATG ACGTTACTCC AAACCCTCTT CCTCCCGTTC AAAATGGAGA AGCCAGCACC 
GGAGAGTATG CCACAAACTT AGCAGAATCT GTAATGCAAG ATGCCTTCAT CCGATTGTCT 
CAGTCACAGC CCACACTCCC CCAGGAATCT GCAGTCAGTT TCTCTATGCG AAGTGCTCTC 
CTTCCCAGTG GCTGTTGCAC AAAAGATATG GTGGTCCCTC GTTCATGGAA CGAGCTACCC 
AAAATTGTCA TTGTGCAGAG CCCAGATGGC AGTGACACAG TCCCTGAGCC AAATGTCTCC 
TCCTGGCCTG ACATGGAATT TGTTGAAACC TCGGGCATCT TTTCTGCAGA CAGTTCCAGC 
AGACCCACTC AGAGTGCCCT AGAAGTTGCA TTGGCTTGTG CAGCCACTGT GATTGGAACC 
ATTTCCAGTC CACAGGCCAC AGAAAGATTC GCCATGGAGC AAGAATCCCT GGTATCTACC 
TATGCCCAGA GAGGCACTGG GGTACAGCAA ACTCAAGTGC CCCAAGCATT CATGGCACCT 
TCTACAACCG AGTATTCCTT CCCATCAGCT CTGTGTGGCA TGACACAAGT GGCAAGTGCT 
GTCGCTGTCT GTGGCTTATG TGAAAAAGAA GAGGCGACAT GTCCTGTAGC TCCAACTGAC 
CTCTTGCCTA CATCTGGGGC TTCTGAAGAA ATATCCTCCA TCGGAAGTTT AGTGATGGAG 
AGGAGCACAG AACTGGGGAA GGAAGCCATT GCAGAGGCTC TGCTCAGAGA GGCTACTCTG 
ATTTTAGCAA GGCCAGATGC TTACAGCAGC CTTGGTGAGC TCTTGGAATC TGTGAACCAG 
AGAATCATAG AAACCACTTC TAAAACCCAG ACCCTGTGCA CAGAAAGTGT TCAAAGGAAT 
GAACTGGCAC ACACCTTGTC CAATGTCATC CTCAAGCACT CCGTGGATGA ACTTCACCAG 
AAAACTACAA TGGCTCACCC CACTGATGAA AGGCATCCCT GTGGAACTCT GGACACCTTG 
ATGGAAAGTG TGAACCAGCT GCTGCACAAT GTGATCTGCT TCACATTCAA AAAGATGAAT 
CACATTGTAA CACTCAGTGA GCATCCCTCA TTTGATCAGG CTGCTGGTCA AGCCTGGGTA 
AAAGCCTTTG CATGCCCCAG CAGCCAGCCT CTTAGCAATG CACACGGTAC TGGCCTTGTC 
ATCAGGAATC TTGTAGAGGA TGCATCTCCT AAGTCAAACA AGGGCGGAGC AAGGCCAGAA 
CTGGTCAACA ACCCCAGGTT GCAATCTGAA TTCTCCTGCA GTCATAGAAT GTTTGATTCA 
ACTGCTAAGT CATTCCCCAA GGAAATATAT CTGAAAGGGA TTATGGGAGA GGATACCAGG 
AACCCTCATC ATACACTAAA TTATGACAGC AATGAACGAA GAGCCTCTAC AGACCTAGGA 
AAATTGACAA CAGCAAGCGA GGGTTGTAGT GGTTTCCAGG AAACTGAAGA CAGCATTGTT 
CCAAACACCC AAGAGAAATA CATCTGTGCC ACACCCCTAA ACAATGAAGC TCAAGTTAAC 
CTATCCTTAT TAGGTGATGA CCTGTCTGTT CCTGCTCAGT CTACTCTAGA GGCAAAGCAG 
TCCGAGGTCT ATGGCATCAC AGATTTTGCG GAAGAATTGG CAGAGACTGT TGTCTCCATG 
GCAACTGAAA TTGCAGCAAT CTGCCTTGAC AACTCCAATG GCAAACAGCC CTGGTTTTGT 
GCTTGGAAAA GAGGGAACGA GTTTTTAACG GCGCCTAACG GATCCTGCCG ATCCTTGAAG 
AGGAAGAAGG AAAACTCAAG CGCCGGAAGC ACCGTGAGGA AACACAAGCC ACCTCGCCTC 
AGTGAAATCA AGAGAAAAGC TGATGAGCAC CCAGAGCTGA AAGAGAAGCT GATGAACAGA 
GTCATGGATG AGTCCATGAA CCTCGAAGAC ATCCCTGATT CCGTCAGTAC CTTTGCCAAT 
GAAGTGGCAG CCAAGATCAT GAACCTCACA GAGTTTTCCA TGGTGGATGG GGTGTGGCAA 
GGCCAGAGTT GTTCCCGGAC TCGGCTTCTG GGTGGCGATA GGTGGAACCG GCTGAAGGCC 
TCAAGCTGTG AGAGCATTCC TGAGGAGGAC TCTGAAGCCA GGGTCTTTGT AAACAGCCTG 
GGTTTGATGA GTACCTTAAG CCAACCAGTT AGCAGGGCCA GCTCCGTCTC CAAACAGTCC 
AGCTGTGAGA GCATCACCGA TGAGTTTTCC AGGTTCATGG TGAAACAGAT GGAAAACGAA 
GGGAGAGGGT TTGAGTTGCT GCTGGATTAC TACGCAGGCA AAAATGCCAG CAGTATCATG 
AGCTCAGCGA TGCAGCAGGC ATGCCAGAAA AACGACCACC TCAACGTGAG ACCGAGCTGC 
CCCTCTAAGC AATCCAGTAC AGAGAGCATA ACAGAGGAGT TCTATAGGTA CATGTTAAGG 
GACATCGCGA AAGAAAGCAA AGATGGTGCC TCCTCCAGAC GTAGCAGCCA TGATTGGACC 
ACGGGCCTGC TGTCTCCTTC TACACGATCC CCTCTGTGTT ACAGACAGTC ATCTATGCCT 
GACAGCAGGT CGCCATGCTC CAGACTAACA GTAAATGCGC CTGTCAAAGC CAACTCCTTA 
GATGGCTTTG CCCAAAACTG CCCTCAAGAT TCTGTAAATG TACAGCCAGT CAGTAGGGCT 
TCCTCATCTG GCCTCTGCAA ATCAGATTCC TGCTTGTATC GCAGAAGTGG GACTGACCAG 
ATCACAAACA TGCTAATTCA TGAAACATGG GCCAGCTCCA TTGAGGCTCT CATGAGAAAG 
AACAAAATCA TTGCTGATGA TAGCGAGGCA GCTAATGCCA GTCCTGGCCC TGTTTCCAGT 
GGTTCTCCTT TGCAAGTAGA GAAGAATGCC AACAGATTAG CCACCAGCAA AGGACACAGG 
GGGCCAACTC TGCTTGTACA GGAATCTGTT GATTACCAGA GAAAAGATGC TGTTACTGAA 
GGCAATCATT CCCCAGTGTC ATCTCCAGGC AAAACAGCTC CTGTTAAAAA ACCCAGTGAT 
TTTGATCCTA GAAGAGAAAC CTCTGCATGC CACAATGCTG CTGGTCTCAA CAGCCCTAGG 
CGGTCACTCT GCTCAAGGGA TGTGCCTTTG ATTCAGATAG AGACAGATCA GAAAGAAGAG 
TGCATTGGAG AACCTGGACC TTTCCTTTCC CAAAGTGGCT CCCTAGAGGA AACAGAAGGC 
CACCAACCTG AAGAAACCAT CCCAGATGTG GCCAGAAATG AAGACACAGC TCCGAGCACC 
TGTCAGAGCT CTCGTGACAG CCTTGAGACC AGTGGGGAAG TAGAAGTGGA GGTCTTGAAA 
GAAGACATAC CCCGAGATGA GTCCCGGAAC CCTCCTAGCA GCAGCGAGGA GAGTACAGGC 
AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC ATCCCAGACG ACACAAGCAG CTTTCTGCAG 
CTCAGCGAGC GGTCCATGAG CAATGGCAAC AGTAGTGGCA CTAGCAGTCT TGGCATTATG 
GACCTGGACA TTTATCAGGA AAGCATACCG TCTTCTCCCA TGATTAATGA ATTAGTAGAA 
GAAAAGGAGA TTCTTAAAGA ACAATCAGAA AGCATAAAGG AACATGCCTC TGGACTGCCA 
GGGAGAGCTG CCAGCCCCCA GAGGAGCCTA CTGGTGATCA ACTTTGACCT GGAGCCAGAG 
TGTCCTGATG CTGAGCTTCG AGCCACTCTG CAGTGGATAG CTGCCTCCGA ACTGGGGATC 
CCAACAATCT ACTTTAAGAA ATCTCAGGAA AGCAGAATTG AAAAGTTTCT AGATGTTGTG 
AAGCTTGTTC AGCAGAAGTC CTGGAAGGTG GGAGATATAT TTCACGCGGT TGTCCAGTAC 
TGCAAACTGC ATGCAGAGCA GAAGGAGAGG ACTCCGAGTC TCTTTGACTG GCTTCTGGAA 
CTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( XM_006496577.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496640.1 Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X3, mRNA.

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CloneID	OMu27828
Clone ID Related Accession (Same CDS sequence)	XM_006496577.3 , XM_006496577.4
Accession Version	XM_006496577.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	A-kinase anchor protein SPHKAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496577.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTCA ACTCCCGGCT TTCTGTGCAA AGCAACGTGG AATCACCACT GATGCATGAA 
GGTTCAGAGC CACAGCAGAT CACCAGCAGT GCAGCAGGCA ACCTGGCTGG CTCCATCACG 
GCCTGTAAAA AGGTTCTTCG AAGCAATAGC TTGCTGGAGT CCACAGACTA CTGGCTGCAG 
AACCAGAGGA CGCCTTGCCA GATTGGGTTT GTGGAAGATG AGTCAGAAAA CTGTGCTTCT 
GTCTGCTTTG TGAACCTGGA TGTCAACAAA GATGCCTGCA TCACAGAGAA CCTGCAGCAG 
AAACTGGTCA ATGTTTCACC GGACCTTCCA AACCTCATCA GCTCCATGAA TGTCCAACAG 
CCGAAAGAAA ATGAAATTGT CCTCCTAAGT GGGTTAGCCT CTGGAAACCT CCAGGCAGAT 
TTTGATGTTT CACAGTGCCC TTGGCTACCA GATATCTGCT TGGTCCAGTG TGCAAGAGGG 
AACAGACCAA ACAGTACCAA CTGCATCATC TTTGAGATCA ACAAGTTCTT AATTGGTCTG 
GAAGTAGTGC AGGAGCGTCA GCTCCACCTG GAGACGAACG TGTTGAAGCT GGAGGATGAT 
ACAAACTGTT CTCTGTCTTC AATTGAAGAA GATTTTCTTA CAGCCTCCGA GCATTTGGAG 
GAAGAAATCG AGGTGGACGA CTGCAGAAGT GGTTTGGAAA ATACAAATGT GTCAGCTAAT 
GTTTTGGAAA GTAAAAAGCC AAAGGAAACT ACCCAGGAAG GATGGGATTA CCACAAGGAA 
AAGTTGCATT GTGCTTTAGG GGAAAAACAC ATTAGAAAAC ACCGTACGCC CTCCACAAAA 
ACAGAAGGGT CAAAGGAAAA TACAGAAGAG AACACGTCCT TGAAAAGTCT CAATCGTTTA 
GTCAGGCCAT CACACCTGAA AAGTGAAGTG GCGGGGAACA AGCAACTGGC CACAAACTAC 
TCCTACCCAG AAAATATCAA AGGGGAACTA GAAACATCTC AAATGCTGTT CATCCCTAGA 
GATGCCTATC TCTCCATGGT TAAGAAAGAT GTACTTTCTC CGTGTAGTGT ACTCAGTGAG 
CAGGGAGGCA GCCACAGAGA CCATGACGTT ACTCCAAACC CTCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA GCACCGGAGA GTATGCCACA AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC ACAGCCCACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
ATGCGAAGTG CTCTCCTTCC CAGTGGCTGT TGCACAAAAG ATATGGTGGT CCCTCGTTCA 
TGGAACGAGC TACCCAAAAT TGTCATTGTG CAGAGCCCAG ATGGCAGTGA CACAGTCCCT 
GAGCCAAATG TCTCCTCCTG GCCTGACATG GAATTTGTTG AAACCTCGGG CATCTTTTCT 
GCAGACAGTT CCAGCAGACC CACTCAGAGT GCCCTAGAAG TTGCATTGGC TTGTGCAGCC 
ACTGTGATTG GAACCATTTC CAGTCCACAG GCCACAGAAA GATTCGCCAT GGAGCAAGAA 
TCCCTGGTAT CTACCTATGC CCAGAGAGGC ACTGGGGTAC AGCAAACTCA AGTGCCCCAA 
GCATTCATGG CACCTTCTAC AACCGAGTAT TCCTTCCCAT CAGCTCTGTG TGGCATGACA 
CAAGTGGCAA GTGCTGTCGC TGTCTGTGGC TTATGTGAAA AAGAAGAGGC GACATGTCCT 
GTAGCTCCAA CTGACCTCTT GCCTACATCT GGGGCTTCTG AAGAAATATC CTCCATCGGA 
AGTTTAGTGA TGGAGAGGAG CACAGAACTG GGGAAGGAAG CCATTGCAGA GGCTCTGCTC 
AGAGAGGCTA CTCTGATTTT AGCAAGGCCA GATGCTTACA GCAGCCTTGG TGAGCTCTTG 
GAATCTGTGA ACCAGAGAAT CATAGAAACC ACTTCTAAAA CCCAGACCCT GTGCACAGAA 
AGTGTTCAAA GGAATGAACT GGCACACACC TTGTCCAATG TCATCCTCAA GCACTCCGTG 
GATGAACTTC ACCAGAAAAC TACAATGGCT CACCCCACTG ATGAAAGGCA TCCCTGTGGA 
ACTCTGGACA CCTTGATGGA AAGTGTGAAC CAGCTGCTGC ACAATGTGAT CTGCTTCACA 
TTCAAAAAGA TGAATCACAT TGTAACACTC AGTGAGCATC CCTCATTTGA TCAGGCTGCT 
GGTCAAGCCT GGGTAAAAGC CTTTGCATGC CCCAGCAGCC AGCCTCTTAG CAATGCACAC 
GGTACTGGCC TTGTCATCAG GAATCTTGTA GAGGATGCAT CTCCTAAGTC AAACAAGGGC 
GGAGCAAGGC CAGAACTGGT CAACAACCCC AGGTTGCAAT CTGAATTCTC CTGCAGTCAT 
AGAATGTTTG ATTCAACTGC TAAGTCATTC CCCAAGGAAA TATATCTGAA AGGGATTATG 
GGAGAGGATA CCAGGAACCC TCATCATACA CTAAATTATG ACAGCAATGA ACGAAGAGCC 
TCTACAGACC TAGGAAAATT GACAACAGCA AGCGAGGGTT GTAGTGGTTT CCAGGAAACT 
GAAGACAGCA TTGTTCCAAA CACCCAAGAG AAATACATCT GTGCCACACC CCTAAACAAT 
GAAGCTCAAG TTAACCTATC CTTATTAGGT GATGACCTGT CTGTTCCTGC TCAGTCTACT 
CTAGAGGCAA AGCAGTCCGA GGTCTATGGC ATCACAGATT TTGCGGAAGA ATTGGCAGAG 
ACTGTTGTCT CCATGGCAAC TGAAATTGCA GCAATCTGCC TTGACAACTC CAATGGCAAA 
CAGCCCTGGT TTTGTGCTTG GAAAAGAGGG AACGAGTTTT TAACGGCGCC TAACGGATCC 
TGCCGATCCT TGAAGAGGAA GAAGGAAAAC TCAAGCGCCG GAAGCACCGT GAGGAAACAC 
AAGCCACCTC GCCTCAGTGA AATCAAGAGA AAAGCTGATG AGCACCCAGA GCTGAAAGAG 
AAGCTGATGA ACAGAGTCAT GGATGAGTCC ATGAACCTCG AAGACATCCC TGATTCCGTC 
AGTACCTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TCACAGAGTT TTCCATGGTG 
GATGGGGTGT GGCAAGGCCA GAGTTGTTCC CGGACTCGGC TTCTGGGTGG CGATAGGTGG 
AACCGGCTGA AGGCCTCAAG CTGTGAGAGC ATTCCTGAGG AGGACTCTGA AGCCAGGGTC 
TTTGTAAACA GCCTGGGTTT GATGAGTACC TTAAGCCAAC CAGTTAGCAG GGCCAGCTCC 
GTCTCCAAAC AGTCCAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAA 
CAGATGGAAA ACGAAGGGAG AGGGTTTGAG TTGCTGCTGG ATTACTACGC AGGCAAAAAT 
GCCAGCAGTA TCATGAGCTC AGCGATGCAG CAGGCATGCC AGAAAAACGA CCACCTCAAC 
GTGAGACCGA GCTGCCCCTC TAAGCAATCC AGTACAGAGA GCATAACAGA GGAGTTCTAT 
AGGTACATGT TAAGGGACAT CGCGAAAGAA AGCAAAGATG GTGCCTCCTC CAGACGTAGC 
AGCCATGATT GGACCACGGG CCTGCTGTCT CCTTCTACAC GATCCCCTCT GTGTTACAGA 
CAGTCATCTA TGCCTGACAG CAGGTCGCCA TGCTCCAGAC TAACAGTAAA TGCGCCTGTC 
AAAGCCAACT CCTTAGATGG CTTTGCCCAA AACTGCCCTC AAGATTCTGT AAATGTACAG 
CCAGTCAGTA GGGCTTCCTC ATCTGGCCTC TGCAAATCAG ATTCCTGCTT GTATCGCAGA 
AGTGGGACTG ACCAGATCAC AAACATGCTA ATTCATGAAA CATGGGCCAG CTCCATTGAG 
GCTCTCATGA GAAAGAACAA AATCATTGCT GATGATAGCG AGGCAGCTAA TGCCAGTCCT 
GGCCCTGTTT CCAGTGGTTC TCCTTTGCAA GTAGAGAAGA ATGCCAACAG ATTAGCCACC 
AGCAAAGGAC ACAGGGGGCC AACTCTGCTT GTACAGGAAT CTGTTGATTA CCAGAGAAAA 
GATGCTGTTA CTGAAGGCAA TCATTCCCCA GTGTCATCTC CAGGCAAAAC AGCTCCTGTT 
AAAAAACCCA GTGATTTTGA TCCTAGAAGA GAAACCTCTG CATGCCACAA TGCTGCTGGT 
CTCAACAGCC CTAGGCGGTC ACTCTGCTCA AGGGATGTGC CTTTGATTCA GATAGAGACA 
GATCAGAAAG AAGAGTGCAT TGGAGAACCT GGACCTTTCC TTTCCCAAAG TGGCTCCCTA 
GAGGAAACAG AAGGCCACCA ACCTGAAGAA ACCATCCCAG ATGTGGCCAG AAATGAAGAC 
ACAGCTCCGA GCACCTGTCA GAGCTCTCGT GACAGCCTTG AGACCAGTGG GGAAGTAGAA 
GTGGAGGTCT TGAAAGAAGA CATACCCCGA GATGAGTCCC GGAACCCTCC TAGCAGCAGC 
GAGGAGAGTA CAGGCAGCTG GTCCCAGCTG GCCAATGAGG AGGACATCCC AGACGACACA 
AGCAGCTTTC TGCAGCTCAG CGAGCGGTCC ATGAGCAATG GCAACAGTAG TGGCACTAGC 
AGTCTTGGCA TTATGGACCT GGACATTTAT CAGGAAAGCA TACCGTCTTC TCCCATGATT 
AATGAATTAG TAGAAGAAAA GGAGATTCTT AAAGAACAAT CAGAAAGCAT AAAGGAACAT 
GCCTCTGGAC TGCCAGGGAG AGCTGCCAGC CCCCAGAGGA GCCTACTGGT GATCAACTTT 
GACCTGGAGC CAGAGTGTCC TGATGCTGAG CTTCGAGCCA CTCTGCAGTG GATAGCTGCC 
TCCGAACTGG GGATCCCAAC AATCTACTTT AAGAAATCTC AGGAAAGCAG AATTGAAAAG 
TTTCTAGATG TTGTGAAGCT TGTTCAGCAG AAGTCCTGGA AGGTGGGAGA TATATTTCAC 
GCGGTTGTCC AGTACTGCAA ACTGCATGCA GAGCAGAAGG AGAGGACTCC GAGTCTCTTT 
GACTGGCTTC TGGAACTAGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496640.1
CDS	401..5464
Translation	MDVNSRLSVQSNVESPLMHEGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASGNLQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETNVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETTQEGWDYHKEKLHCALGEKHIRKHRTPSTKTEGSKENTEENTSLKSLNRLVRPSHLKSEVAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKDVLSPCSVLSEQGGSHRDHDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSMRSALLPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADSSSRPTQSALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQAFMAPSTTEYSFPSALCGMTQVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSIGSLVMERSTELGKEAIAEALLREATLILARPDAYSSLGELLESVNQRIIETTSKTQTLCTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCGTLDTLMESVNQLLHNVICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLVEDASPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTLNYDSNERRASTDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLLGDDLSVPAQSTLEAKQSEVYGITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCSRTRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEFYRYMLRDIAKESKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVNAPVKANSLDGFAQNCPQDSVNVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQVEKNANRLATSKGHRGPTLLVQESVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAGLNSPRRSLCSRDVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSSRDSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLSERSMSNGNSSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESIKEHASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006496577.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496577.4

ORF Insert Sequence:

ATGGATGTCA ACTCCCGGCT TTCTGTGCAA AGCAACGTGG AATCACCACT GATGCATGAA 
GGTTCAGAGC CACAGCAGAT CACCAGCAGT GCAGCAGGCA ACCTGGCTGG CTCCATCACG 
GCCTGTAAAA AGGTTCTTCG AAGCAATAGC TTGCTGGAGT CCACAGACTA CTGGCTGCAG 
AACCAGAGGA CGCCTTGCCA GATTGGGTTT GTGGAAGATG AGTCAGAAAA CTGTGCTTCT 
GTCTGCTTTG TGAACCTGGA TGTCAACAAA GATGCCTGCA TCACAGAGAA CCTGCAGCAG 
AAACTGGTCA ATGTTTCACC GGACCTTCCA AACCTCATCA GCTCCATGAA TGTCCAACAG 
CCGAAAGAAA ATGAAATTGT CCTCCTAAGT GGGTTAGCCT CTGGAAACCT CCAGGCAGAT 
TTTGATGTTT CACAGTGCCC TTGGCTACCA GATATCTGCT TGGTCCAGTG TGCAAGAGGG 
AACAGACCAA ACAGTACCAA CTGCATCATC TTTGAGATCA ACAAGTTCTT AATTGGTCTG 
GAAGTAGTGC AGGAGCGTCA GCTCCACCTG GAGACGAACG TGTTGAAGCT GGAGGATGAT 
ACAAACTGTT CTCTGTCTTC AATTGAAGAA GATTTTCTTA CAGCCTCCGA GCATTTGGAG 
GAAGAAATCG AGGTGGACGA CTGCAGAAGT GGTTTGGAAA ATACAAATGT GTCAGCTAAT 
GTTTTGGAAA GTAAAAAGCC AAAGGAAACT ACCCAGGAAG GATGGGATTA CCACAAGGAA 
AAGTTGCATT GTGCTTTAGG GGAAAAACAC ATTAGAAAAC ACCGTACGCC CTCCACAAAA 
ACAGAAGGGT CAAAGGAAAA TACAGAAGAG AACACGTCCT TGAAAAGTCT CAATCGTTTA 
GTCAGGCCAT CACACCTGAA AAGTGAAGTG GCGGGGAACA AGCAACTGGC CACAAACTAC 
TCCTACCCAG AAAATATCAA AGGGGAACTA GAAACATCTC AAATGCTGTT CATCCCTAGA 
GATGCCTATC TCTCCATGGT TAAGAAAGAT GTACTTTCTC CGTGTAGTGT ACTCAGTGAG 
CAGGGAGGCA GCCACAGAGA CCATGACGTT ACTCCAAACC CTCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA GCACCGGAGA GTATGCCACA AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC ACAGCCCACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
ATGCGAAGTG CTCTCCTTCC CAGTGGCTGT TGCACAAAAG ATATGGTGGT CCCTCGTTCA 
TGGAACGAGC TACCCAAAAT TGTCATTGTG CAGAGCCCAG ATGGCAGTGA CACAGTCCCT 
GAGCCAAATG TCTCCTCCTG GCCTGACATG GAATTTGTTG AAACCTCGGG CATCTTTTCT 
GCAGACAGTT CCAGCAGACC CACTCAGAGT GCCCTAGAAG TTGCATTGGC TTGTGCAGCC 
ACTGTGATTG GAACCATTTC CAGTCCACAG GCCACAGAAA GATTCGCCAT GGAGCAAGAA 
TCCCTGGTAT CTACCTATGC CCAGAGAGGC ACTGGGGTAC AGCAAACTCA AGTGCCCCAA 
GCATTCATGG CACCTTCTAC AACCGAGTAT TCCTTCCCAT CAGCTCTGTG TGGCATGACA 
CAAGTGGCAA GTGCTGTCGC TGTCTGTGGC TTATGTGAAA AAGAAGAGGC GACATGTCCT 
GTAGCTCCAA CTGACCTCTT GCCTACATCT GGGGCTTCTG AAGAAATATC CTCCATCGGA 
AGTTTAGTGA TGGAGAGGAG CACAGAACTG GGGAAGGAAG CCATTGCAGA GGCTCTGCTC 
AGAGAGGCTA CTCTGATTTT AGCAAGGCCA GATGCTTACA GCAGCCTTGG TGAGCTCTTG 
GAATCTGTGA ACCAGAGAAT CATAGAAACC ACTTCTAAAA CCCAGACCCT GTGCACAGAA 
AGTGTTCAAA GGAATGAACT GGCACACACC TTGTCCAATG TCATCCTCAA GCACTCCGTG 
GATGAACTTC ACCAGAAAAC TACAATGGCT CACCCCACTG ATGAAAGGCA TCCCTGTGGA 
ACTCTGGACA CCTTGATGGA AAGTGTGAAC CAGCTGCTGC ACAATGTGAT CTGCTTCACA 
TTCAAAAAGA TGAATCACAT TGTAACACTC AGTGAGCATC CCTCATTTGA TCAGGCTGCT 
GGTCAAGCCT GGGTAAAAGC CTTTGCATGC CCCAGCAGCC AGCCTCTTAG CAATGCACAC 
GGTACTGGCC TTGTCATCAG GAATCTTGTA GAGGATGCAT CTCCTAAGTC AAACAAGGGC 
GGAGCAAGGC CAGAACTGGT CAACAACCCC AGGTTGCAAT CTGAATTCTC CTGCAGTCAT 
AGAATGTTTG ATTCAACTGC TAAGTCATTC CCCAAGGAAA TATATCTGAA AGGGATTATG 
GGAGAGGATA CCAGGAACCC TCATCATACA CTAAATTATG ACAGCAATGA ACGAAGAGCC 
TCTACAGACC TAGGAAAATT GACAACAGCA AGCGAGGGTT GTAGTGGTTT CCAGGAAACT 
GAAGACAGCA TTGTTCCAAA CACCCAAGAG AAATACATCT GTGCCACACC CCTAAACAAT 
GAAGCTCAAG TTAACCTATC CTTATTAGGT GATGACCTGT CTGTTCCTGC TCAGTCTACT 
CTAGAGGCAA AGCAGTCCGA GGTCTATGGC ATCACAGATT TTGCGGAAGA ATTGGCAGAG 
ACTGTTGTCT CCATGGCAAC TGAAATTGCA GCAATCTGCC TTGACAACTC CAATGGCAAA 
CAGCCCTGGT TTTGTGCTTG GAAAAGAGGG AACGAGTTTT TAACGGCGCC TAACGGATCC 
TGCCGATCCT TGAAGAGGAA GAAGGAAAAC TCAAGCGCCG GAAGCACCGT GAGGAAACAC 
AAGCCACCTC GCCTCAGTGA AATCAAGAGA AAAGCTGATG AGCACCCAGA GCTGAAAGAG 
AAGCTGATGA ACAGAGTCAT GGATGAGTCC ATGAACCTCG AAGACATCCC TGATTCCGTC 
AGTACCTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TCACAGAGTT TTCCATGGTG 
GATGGGGTGT GGCAAGGCCA GAGTTGTTCC CGGACTCGGC TTCTGGGTGG CGATAGGTGG 
AACCGGCTGA AGGCCTCAAG CTGTGAGAGC ATTCCTGAGG AGGACTCTGA AGCCAGGGTC 
TTTGTAAACA GCCTGGGTTT GATGAGTACC TTAAGCCAAC CAGTTAGCAG GGCCAGCTCC 
GTCTCCAAAC AGTCCAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAA 
CAGATGGAAA ACGAAGGGAG AGGGTTTGAG TTGCTGCTGG ATTACTACGC AGGCAAAAAT 
GCCAGCAGTA TCATGAGCTC AGCGATGCAG CAGGCATGCC AGAAAAACGA CCACCTCAAC 
GTGAGACCGA GCTGCCCCTC TAAGCAATCC AGTACAGAGA GCATAACAGA GGAGTTCTAT 
AGGTACATGT TAAGGGACAT CGCGAAAGAA AGCAAAGATG GTGCCTCCTC CAGACGTAGC 
AGCCATGATT GGACCACGGG CCTGCTGTCT CCTTCTACAC GATCCCCTCT GTGTTACAGA 
CAGTCATCTA TGCCTGACAG CAGGTCGCCA TGCTCCAGAC TAACAGTAAA TGCGCCTGTC 
AAAGCCAACT CCTTAGATGG CTTTGCCCAA AACTGCCCTC AAGATTCTGT AAATGTACAG 
CCAGTCAGTA GGGCTTCCTC ATCTGGCCTC TGCAAATCAG ATTCCTGCTT GTATCGCAGA 
AGTGGGACTG ACCAGATCAC AAACATGCTA ATTCATGAAA CATGGGCCAG CTCCATTGAG 
GCTCTCATGA GAAAGAACAA AATCATTGCT GATGATAGCG AGGCAGCTAA TGCCAGTCCT 
GGCCCTGTTT CCAGTGGTTC TCCTTTGCAA GTAGAGAAGA ATGCCAACAG ATTAGCCACC 
AGCAAAGGAC ACAGGGGGCC AACTCTGCTT GTACAGGAAT CTGTTGATTA CCAGAGAAAA 
GATGCTGTTA CTGAAGGCAA TCATTCCCCA GTGTCATCTC CAGGCAAAAC AGCTCCTGTT 
AAAAAACCCA GTGATTTTGA TCCTAGAAGA GAAACCTCTG CATGCCACAA TGCTGCTGGT 
CTCAACAGCC CTAGGCGGTC ACTCTGCTCA AGGGATGTGC CTTTGATTCA GATAGAGACA 
GATCAGAAAG AAGAGTGCAT TGGAGAACCT GGACCTTTCC TTTCCCAAAG TGGCTCCCTA 
GAGGAAACAG AAGGCCACCA ACCTGAAGAA ACCATCCCAG ATGTGGCCAG AAATGAAGAC 
ACAGCTCCGA GCACCTGTCA GAGCTCTCGT GACAGCCTTG AGACCAGTGG GGAAGTAGAA 
GTGGAGGTCT TGAAAGAAGA CATACCCCGA GATGAGTCCC GGAACCCTCC TAGCAGCAGC 
GAGGAGAGTA CAGGCAGCTG GTCCCAGCTG GCCAATGAGG AGGACATCCC AGACGACACA 
AGCAGCTTTC TGCAGCTCAG CGAGCGGTCC ATGAGCAATG GCAACAGTAG TGGCACTAGC 
AGTCTTGGCA TTATGGACCT GGACATTTAT CAGGAAAGCA TACCGTCTTC TCCCATGATT 
AATGAATTAG TAGAAGAAAA GGAGATTCTT AAAGAACAAT CAGAAAGCAT AAAGGAACAT 
GCCTCTGGAC TGCCAGGGAG AGCTGCCAGC CCCCAGAGGA GCCTACTGGT GATCAACTTT 
GACCTGGAGC CAGAGTGTCC TGATGCTGAG CTTCGAGCCA CTCTGCAGTG GATAGCTGCC 
TCCGAACTGG GGATCCCAAC AATCTACTTT AAGAAATCTC AGGAAAGCAG AATTGAAAAG 
TTTCTAGATG TTGTGAAGCT TGTTCAGCAG AAGTCCTGGA AGGTGGGAGA TATATTTCAC 
GCGGTTGTCC AGTACTGCAA ACTGCATGCA GAGCAGAAGG AGAGGACTCC GAGTCTCTTT 
GACTGGCTTC TGGAACTAGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( NM_172430.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766018.3 Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant 2, mRNA.

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CloneID	OMu03435
Clone ID Related Accession (Same CDS sequence)	NM_172430.4 , NM_172430.3
Accession Version	NM_172430.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	A-kinase anchor protein SPHKAP isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY769819.1, BY774616.1, AK038638.1, AK122538.1, BC069832.1 and BC060165.1. On Oct 7, 2017 this sequence version replaced NM_172430.3. ##Evidence-Data-START## Transcript exon combination :: BC069832.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849377 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGTCA ACTCCCGGCT TTCTGTGCAA AGCAACGTGG AATCACCACT GATGCATGAA 
GGTTCAGAGC CACAGCAGAT CACCAGCAGT GCAGCAGGCA ACCTGGCTGG CTCCATCACG 
GCCTGTAAAA AGGTTCTTCG AAGCAATAGC TTGCTGGAGT CCACAGACTA CTGGCTGCAG 
AACCAGAGGA CGCCTTGCCA GATTGGGTTT GTGGAAGATG AGTCAGAAAA CTGTGCTTCT 
GTCTGCTTTG TGAACCTGGA TGTCAACAAA GATGCCTGCA TCACAGAGAA CCTGCAGCAG 
AAACTGGTCA ATGTTTCACC GGACCTTCCA AACCTCATCA GCTCCATGAA TGTCCAACAG 
CCGAAAGAAA ATGAAATTGT CCTCCTAAGT GGGTTAGCCT CTGGAAACCT CCAGGCAGAT 
TTTGATGTTT CACAGTGCCC TTGGCTACCA GATATCTGCT TGGTCCAGTG TGCAAGAGGG 
AACAGACCAA ACAGTACCAA CTGCATCATC TTTGAGATCA ACAAGTTCTT AATTGGTCTG 
GAAGTAGTGC AGGAGCGTCA GCTCCACCTG GAGACGAACG TGTTGAAGCT GGAGGATGAT 
ACAAACTGTT CTCTGTCTTC AATTGAAGAA GATTTTCTTA CAGCCTCCGA GCATTTGGAG 
GAAGAAATCG AGGTGGACGA CTGCAGAAGT GGTTTGGAAA ATACAAATGT GTCAGCTAAT 
GTTTTGGAAA GTAAAAAGCC AAAGGAAACT ACCCAGGAAG GATGGGATTA CCACAAGGAA 
AAGTTGCATT GTGCTTTAGG GGAAAAACAC ATTAGAAAAC ACCGTACGCC CTCCACAAAA 
ACAGAAGGGT CAAAGGAAAA TACAGAAGAG AACACGTCCT TGAAAAGTCT CAATCGTTTA 
GTCAGGCCAT CACACCTGAA AAGTGAAGTG GCGGGGAACA AGCAACTGGC CACAAACTAC 
TCCTACCCAG AAAATATCAA AGGGGAACTA GAAACATCTC AAATGCTGTT CATCCCTAGA 
GATGCCTATC TCTCCATGGT TAAGAAAGAT GTACTTTCTC CGTGTAGTGT ACTCAGTGAG 
CAGGGAGGCA GCCACAGAGA CCATGACGTT ACTCCAAACC CTCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA GCACCGGAGA GTATGCCACA AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC ACAGCCCACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
ATGCGAAGTG CTCTCCTTCC CAGTGGCTGT TGCACAAAAG ATATGGTGGT CCCTCGTTCA 
TGGAACGAGC TACCCAAAAT TGTCATTGTG CAGAGCCCAG ATGGCAGTGA CACAGTCCCT 
GAGCCAAATG TCTCCTCCTG GCCTGACATG GAATTTGTTG AAACCTCGGG CATCTTTTCT 
GCAGACAGTT CCAGCAGACC CACTCAGAGT GCCCTAGAAG TTGCATTGGC TTGTGCAGCC 
ACTGTGATTG GAACCATTTC CAGTCCACAG GCCACAGAAA GATTCGCCAT GGAGCAAGAA 
TCCCTGGTAT CTACCTATGC CCAGAGAGGC ACTGGGGTAC AGCAAACTCA AGTGCCCCAA 
GCATTCATGG CACCTTCTAC AACCGAGTAT TCCTTCCCAT CAGCTCTGTG TGGCATGACA 
CAAGTGGCAA GTGCTGTCGC TGTCTGTGGC TTATGTGAAA AAGAAGAGGC GACATGTCCT 
GTAGCTCCAA CTGACCTCTT GCCTACATCT GGGGCTTCTG AAGAAATATC CTCCATCGGA 
AGTTTAGTGA TGGAGAGGAG CACAGAACTG GGGAAGGAAG CCATTGCAGA GGCTCTGCTC 
AGAGAGGCTA CTCTGATTTT AGCAAGGCCA GATGCTTACA GCAGCCTTGG TGAGCTCTTG 
GAATCTGTGA ACCAGAGAAT CATAGAAACC ACTTCTAAAA CCCAGACCCT GTGCACAGAA 
AGTGTTCAAA GGAATGAACT GGCACACACC TTGTCCAATG TCATCCTCAA GCACTCCGTG 
GATGAACTTC ACCAGAAAAC TACAATGGCT CACCCCACTG ATGAAAGGCA TCCCTGTGGA 
ACTCTGGACA CCTTGATGGA AAGTGTGAAC CAGCTGCTGC ACAATGTGAT CTGCTTCACA 
TTCAAAAAGA TGAATCACAT TGTAACACTC AGTGAGCATC CCTCATTTGA TCAGGCTGCT 
GGTCAAGCCT GGGTAAAAGC CTTTGCATGC CCCAGCAGCC AGCCTCTTAG CAATGCACAC 
GGTACTGGCC TTGTCATCAG GAATCTTGTA GAGGATGCAT CTCCTAAGTC AAACAAGGGC 
GGAGCAAGGC CAGAACTGGT CAACAACCCC AGGTTGCAAT CTGAATTCTC CTGCAGTCAT 
AGAATGTTTG ATTCAACTGC TAAGTCATTC CCCAAGGAAA TATATCTGAA AGGGATTATG 
GGAGAGGATA CCAGGAACCC TCATCATACA CTAAATTATG ACAGCAATGA ACGAAGAGCC 
TCTACAGACC TAGGAAAATT GACAACAGCA AGCGAGGGTT GTAGTGGTTT CCAGGAAACT 
GAAGACAGCA TTGTTCCAAA CACCCAAGAG AAATACATCT GTGCCACACC CCTAAACAAT 
GAAGCTCAAG TTAACCTATC CTTATTAGGT GATGACCTGT CTGTTCCTGC TCAGTCTACT 
CTAGAGGCAA AGCAGTCCGA GGTCTATGGC ATCACAGATT TTGCGGAAGA ATTGGCAGAG 
ACTGTTGTCT CCATGGCAAC TGAAATTGCA GCAATCTGCC TTGACAACTC CAATGGCAAA 
CAGCCCTGGT TTTGTGCTTG GAAAAGAGGG AACGAGTTTT TAACGGCGCC TAACGGATCC 
TGCCGATCCT TGAAGAGGAA GAAGGAAAAC TCAAGCGCCG GAAGCACCGT GAGGAAACAC 
AAGCCACCTC GCCTCAGTGA AATCAAGAGA AAAGCTGATG AGCACCCAGA GCTGAAAGAG 
AAGCTGATGA ACAGAGTCAT GGATGAGTCC ATGAACCTCG AAGACATCCC TGATTCCGTC 
AGTACCTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TCACAGAGTT TTCCATGGTG 
GATGGGGTGT GGCAAGGCCA GAGTTGTTCC CGGACTCGGC TTCTGGGTGG CGATAGGTGG 
AACCGGCTGA AGGCCTCAAG CTGTGAGAGC ATTCCTGAGG AGGACTCTGA AGCCAGGGTC 
TTTGTAAACA GCCTGGGTTT GATGAGTACC TTAAGCCAAC CAGTTAGCAG GGCCAGCTCC 
GTCTCCAAAC AGTCCAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAA 
CAGATGGAAA ACGAAGGGAG AGGGTTTGAG TTGCTGCTGG ATTACTACGC AGGCAAAAAT 
GCCAGCAGTA TCATGAGCTC AGCGATGCAG CAGGCATGCC AGAAAAACGA CCACCTCAAC 
GTGAGACCGA GCTGCCCCTC TAAGCAATCC AGTACAGAGA GCATAACAGA GGAGTTCTAT 
AGGTACATGT TAAGGGACAT CGCGAAAGAA AGCAAAGATG GTGCCTCCTC CAGACGTAGC 
AGCCATGATT GGACCACGGG CCTGCTGTCT CCTTCTACAC GATCCCCTCT GTGTTACAGA 
CAGTCATCTA TGCCTGACAG CAGGTCGCCA TGCTCCAGAC TAACAGTAAA TGCGCCTGTC 
AAAGCCAACT CCTTAGATGG CTTTGCCCAA AACTGCCCTC AAGATTCTGT AAATGTACAG 
CCAGTCAGTA GGGCTTCCTC ATCTGGCCTC TGCAAATCAG ATTCCTGCTT GTATCGCAGA 
AGTGGGACTG ACCAGATCAC AAACATGCTA ATTCATGAAA CATGGGCCAG CTCCATTGAG 
GCTCTCATGA GAAAGAACAA AATCATTGCT GATGATAGCG AGGCAGCTAA TGCCAGTCCT 
GGCCCTGTTT CCAGTGGTTC TCCTTTGCAA GTAGAGAAGA ATGCCAACAG ATTAGCCACC 
AGCAAAGGAC ACAGGGGGCC AACTCTGCTT GTACAGGAAT CTGTTGATTA CCAGAGAAAA 
GATGCTGTTA CTGAAGGCAA TCATTCCCCA GTGTCATCTC CAGGCAAAAC AGCTCCTGTT 
AAAAAACCCA GTGATTTTGA TCCTAGAAGA GAAACCTCTG CATGCCACAA TGCTGCTGGT 
CTCAACAGCC CTAGGCGGTC ACTCTGCTCA AGGGATGTGC CTTTGATTCA GATAGAGACA 
GATCAGAAAG AAGAGTGCAT TGGAGAACCT GGACCTTTCC TTTCCCAAAG TGGCTCCCTA 
GAGGAAACAG AAGGCCACCA ACCTGAAGAA ACCATCCCAG ATGTGGCCAG AAATGAAGAC 
ACAGCTCCGA GCACCTGTCA GAGCTCTCGT GACAGCCTTG AGACCAGTGG GGAAGTAGAA 
GTGGAGGTCT TGAAAGAAGA CATACCCCGA GATGAGTCCC GGAACCCTCC TAGCAGCAGC 
GAGGAGAGTA CAGGCAGCTG GTCCCAGCTG GCCAATGAGG AGGACATCCC AGACGACACA 
AGCAGCTTTC TGCAGCTCAG CGAGCGGTCC ATGAGTGAAT TAGTAGAAGA AAAGGAGATT 
CTTAAAGAAC AATCAGAAAG CATAAAGGAA CATGCCTCTG GACTGCCAGG GAGAGCTGCC 
AGCCCCCAGA GGAGCCTACT GGTGATCAAC TTTGACCTGG AGCCAGAGTG TCCTGATGCT 
GAGCTTCGAG CCACTCTGCA GTGGATAGCT GCCTCCGAAC TGGGGATCCC AACAATCTAC 
TTTAAGAAAT CTCAGGAAAG CAGAATTGAA AAGTTTCTAG ATGTTGTGAA GCTTGTTCAG 
CAGAAGTCCT GGAAGGTGGG AGATATATTT CACGCGGTTG TCCAGTACTG CAAACTGCAT 
GCAGAGCAGA AGGAGAGGAC TCCGAGTCTC TTTGACTGGC TTCTGGAACT AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766018.3
CDS	190..5166
Translation	MDVNSRLSVQSNVESPLMHEGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASGNLQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETNVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETTQEGWDYHKEKLHCALGEKHIRKHRTPSTKTEGSKENTEENTSLKSLNRLVRPSHLKSEVAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKDVLSPCSVLSEQGGSHRDHDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSMRSALLPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADSSSRPTQSALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQAFMAPSTTEYSFPSALCGMTQVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSIGSLVMERSTELGKEAIAEALLREATLILARPDAYSSLGELLESVNQRIIETTSKTQTLCTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCGTLDTLMESVNQLLHNVICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLVEDASPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTLNYDSNERRASTDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLLGDDLSVPAQSTLEAKQSEVYGITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCSRTRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEFYRYMLRDIAKESKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVNAPVKANSLDGFAQNCPQDSVNVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQVEKNANRLATSKGHRGPTLLVQESVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAGLNSPRRSLCSRDVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSSRDSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLSERSMSELVEEKEILKEQSESIKEHASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG

Target ORF information:

RefSeq Version	NM_172430.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172430.4

ORF Insert Sequence:

ATGGATGTCA ACTCCCGGCT TTCTGTGCAA AGCAACGTGG AATCACCACT GATGCATGAA 
GGTTCAGAGC CACAGCAGAT CACCAGCAGT GCAGCAGGCA ACCTGGCTGG CTCCATCACG 
GCCTGTAAAA AGGTTCTTCG AAGCAATAGC TTGCTGGAGT CCACAGACTA CTGGCTGCAG 
AACCAGAGGA CGCCTTGCCA GATTGGGTTT GTGGAAGATG AGTCAGAAAA CTGTGCTTCT 
GTCTGCTTTG TGAACCTGGA TGTCAACAAA GATGCCTGCA TCACAGAGAA CCTGCAGCAG 
AAACTGGTCA ATGTTTCACC GGACCTTCCA AACCTCATCA GCTCCATGAA TGTCCAACAG 
CCGAAAGAAA ATGAAATTGT CCTCCTAAGT GGGTTAGCCT CTGGAAACCT CCAGGCAGAT 
TTTGATGTTT CACAGTGCCC TTGGCTACCA GATATCTGCT TGGTCCAGTG TGCAAGAGGG 
AACAGACCAA ACAGTACCAA CTGCATCATC TTTGAGATCA ACAAGTTCTT AATTGGTCTG 
GAAGTAGTGC AGGAGCGTCA GCTCCACCTG GAGACGAACG TGTTGAAGCT GGAGGATGAT 
ACAAACTGTT CTCTGTCTTC AATTGAAGAA GATTTTCTTA CAGCCTCCGA GCATTTGGAG 
GAAGAAATCG AGGTGGACGA CTGCAGAAGT GGTTTGGAAA ATACAAATGT GTCAGCTAAT 
GTTTTGGAAA GTAAAAAGCC AAAGGAAACT ACCCAGGAAG GATGGGATTA CCACAAGGAA 
AAGTTGCATT GTGCTTTAGG GGAAAAACAC ATTAGAAAAC ACCGTACGCC CTCCACAAAA 
ACAGAAGGGT CAAAGGAAAA TACAGAAGAG AACACGTCCT TGAAAAGTCT CAATCGTTTA 
GTCAGGCCAT CACACCTGAA AAGTGAAGTG GCGGGGAACA AGCAACTGGC CACAAACTAC 
TCCTACCCAG AAAATATCAA AGGGGAACTA GAAACATCTC AAATGCTGTT CATCCCTAGA 
GATGCCTATC TCTCCATGGT TAAGAAAGAT GTACTTTCTC CGTGTAGTGT ACTCAGTGAG 
CAGGGAGGCA GCCACAGAGA CCATGACGTT ACTCCAAACC CTCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA GCACCGGAGA GTATGCCACA AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC ACAGCCCACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
ATGCGAAGTG CTCTCCTTCC CAGTGGCTGT TGCACAAAAG ATATGGTGGT CCCTCGTTCA 
TGGAACGAGC TACCCAAAAT TGTCATTGTG CAGAGCCCAG ATGGCAGTGA CACAGTCCCT 
GAGCCAAATG TCTCCTCCTG GCCTGACATG GAATTTGTTG AAACCTCGGG CATCTTTTCT 
GCAGACAGTT CCAGCAGACC CACTCAGAGT GCCCTAGAAG TTGCATTGGC TTGTGCAGCC 
ACTGTGATTG GAACCATTTC CAGTCCACAG GCCACAGAAA GATTCGCCAT GGAGCAAGAA 
TCCCTGGTAT CTACCTATGC CCAGAGAGGC ACTGGGGTAC AGCAAACTCA AGTGCCCCAA 
GCATTCATGG CACCTTCTAC AACCGAGTAT TCCTTCCCAT CAGCTCTGTG TGGCATGACA 
CAAGTGGCAA GTGCTGTCGC TGTCTGTGGC TTATGTGAAA AAGAAGAGGC GACATGTCCT 
GTAGCTCCAA CTGACCTCTT GCCTACATCT GGGGCTTCTG AAGAAATATC CTCCATCGGA 
AGTTTAGTGA TGGAGAGGAG CACAGAACTG GGGAAGGAAG CCATTGCAGA GGCTCTGCTC 
AGAGAGGCTA CTCTGATTTT AGCAAGGCCA GATGCTTACA GCAGCCTTGG TGAGCTCTTG 
GAATCTGTGA ACCAGAGAAT CATAGAAACC ACTTCTAAAA CCCAGACCCT GTGCACAGAA 
AGTGTTCAAA GGAATGAACT GGCACACACC TTGTCCAATG TCATCCTCAA GCACTCCGTG 
GATGAACTTC ACCAGAAAAC TACAATGGCT CACCCCACTG ATGAAAGGCA TCCCTGTGGA 
ACTCTGGACA CCTTGATGGA AAGTGTGAAC CAGCTGCTGC ACAATGTGAT CTGCTTCACA 
TTCAAAAAGA TGAATCACAT TGTAACACTC AGTGAGCATC CCTCATTTGA TCAGGCTGCT 
GGTCAAGCCT GGGTAAAAGC CTTTGCATGC CCCAGCAGCC AGCCTCTTAG CAATGCACAC 
GGTACTGGCC TTGTCATCAG GAATCTTGTA GAGGATGCAT CTCCTAAGTC AAACAAGGGC 
GGAGCAAGGC CAGAACTGGT CAACAACCCC AGGTTGCAAT CTGAATTCTC CTGCAGTCAT 
AGAATGTTTG ATTCAACTGC TAAGTCATTC CCCAAGGAAA TATATCTGAA AGGGATTATG 
GGAGAGGATA CCAGGAACCC TCATCATACA CTAAATTATG ACAGCAATGA ACGAAGAGCC 
TCTACAGACC TAGGAAAATT GACAACAGCA AGCGAGGGTT GTAGTGGTTT CCAGGAAACT 
GAAGACAGCA TTGTTCCAAA CACCCAAGAG AAATACATCT GTGCCACACC CCTAAACAAT 
GAAGCTCAAG TTAACCTATC CTTATTAGGT GATGACCTGT CTGTTCCTGC TCAGTCTACT 
CTAGAGGCAA AGCAGTCCGA GGTCTATGGC ATCACAGATT TTGCGGAAGA ATTGGCAGAG 
ACTGTTGTCT CCATGGCAAC TGAAATTGCA GCAATCTGCC TTGACAACTC CAATGGCAAA 
CAGCCCTGGT TTTGTGCTTG GAAAAGAGGG AACGAGTTTT TAACGGCGCC TAACGGATCC 
TGCCGATCCT TGAAGAGGAA GAAGGAAAAC TCAAGCGCCG GAAGCACCGT GAGGAAACAC 
AAGCCACCTC GCCTCAGTGA AATCAAGAGA AAAGCTGATG AGCACCCAGA GCTGAAAGAG 
AAGCTGATGA ACAGAGTCAT GGATGAGTCC ATGAACCTCG AAGACATCCC TGATTCCGTC 
AGTACCTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TCACAGAGTT TTCCATGGTG 
GATGGGGTGT GGCAAGGCCA GAGTTGTTCC CGGACTCGGC TTCTGGGTGG CGATAGGTGG 
AACCGGCTGA AGGCCTCAAG CTGTGAGAGC ATTCCTGAGG AGGACTCTGA AGCCAGGGTC 
TTTGTAAACA GCCTGGGTTT GATGAGTACC TTAAGCCAAC CAGTTAGCAG GGCCAGCTCC 
GTCTCCAAAC AGTCCAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAA 
CAGATGGAAA ACGAAGGGAG AGGGTTTGAG TTGCTGCTGG ATTACTACGC AGGCAAAAAT 
GCCAGCAGTA TCATGAGCTC AGCGATGCAG CAGGCATGCC AGAAAAACGA CCACCTCAAC 
GTGAGACCGA GCTGCCCCTC TAAGCAATCC AGTACAGAGA GCATAACAGA GGAGTTCTAT 
AGGTACATGT TAAGGGACAT CGCGAAAGAA AGCAAAGATG GTGCCTCCTC CAGACGTAGC 
AGCCATGATT GGACCACGGG CCTGCTGTCT CCTTCTACAC GATCCCCTCT GTGTTACAGA 
CAGTCATCTA TGCCTGACAG CAGGTCGCCA TGCTCCAGAC TAACAGTAAA TGCGCCTGTC 
AAAGCCAACT CCTTAGATGG CTTTGCCCAA AACTGCCCTC AAGATTCTGT AAATGTACAG 
CCAGTCAGTA GGGCTTCCTC ATCTGGCCTC TGCAAATCAG ATTCCTGCTT GTATCGCAGA 
AGTGGGACTG ACCAGATCAC AAACATGCTA ATTCATGAAA CATGGGCCAG CTCCATTGAG 
GCTCTCATGA GAAAGAACAA AATCATTGCT GATGATAGCG AGGCAGCTAA TGCCAGTCCT 
GGCCCTGTTT CCAGTGGTTC TCCTTTGCAA GTAGAGAAGA ATGCCAACAG ATTAGCCACC 
AGCAAAGGAC ACAGGGGGCC AACTCTGCTT GTACAGGAAT CTGTTGATTA CCAGAGAAAA 
GATGCTGTTA CTGAAGGCAA TCATTCCCCA GTGTCATCTC CAGGCAAAAC AGCTCCTGTT 
AAAAAACCCA GTGATTTTGA TCCTAGAAGA GAAACCTCTG CATGCCACAA TGCTGCTGGT 
CTCAACAGCC CTAGGCGGTC ACTCTGCTCA AGGGATGTGC CTTTGATTCA GATAGAGACA 
GATCAGAAAG AAGAGTGCAT TGGAGAACCT GGACCTTTCC TTTCCCAAAG TGGCTCCCTA 
GAGGAAACAG AAGGCCACCA ACCTGAAGAA ACCATCCCAG ATGTGGCCAG AAATGAAGAC 
ACAGCTCCGA GCACCTGTCA GAGCTCTCGT GACAGCCTTG AGACCAGTGG GGAAGTAGAA 
GTGGAGGTCT TGAAAGAAGA CATACCCCGA GATGAGTCCC GGAACCCTCC TAGCAGCAGC 
GAGGAGAGTA CAGGCAGCTG GTCCCAGCTG GCCAATGAGG AGGACATCCC AGACGACACA 
AGCAGCTTTC TGCAGCTCAG CGAGCGGTCC ATGAGTGAAT TAGTAGAAGA AAAGGAGATT 
CTTAAAGAAC AATCAGAAAG CATAAAGGAA CATGCCTCTG GACTGCCAGG GAGAGCTGCC 
AGCCCCCAGA GGAGCCTACT GGTGATCAAC TTTGACCTGG AGCCAGAGTG TCCTGATGCT 
GAGCTTCGAG CCACTCTGCA GTGGATAGCT GCCTCCGAAC TGGGGATCCC AACAATCTAC 
TTTAAGAAAT CTCAGGAAAG CAGAATTGAA AAGTTTCTAG ATGTTGTGAA GCTTGTTCAG 
CAGAAGTCCT GGAAGGTGGG AGATATATTT CACGCGGTTG TCCAGTACTG CAAACTGCAT 
GCAGAGCAGA AGGAGAGGAC TCCGAGTCTC TTTGACTGGC TTCTGGAACT AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( NM_172430.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766018.3 Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), mRNA.

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CloneID	OMu03435
Clone ID Related Accession (Same CDS sequence)	NM_172430.4 , NM_172430.3
Accession Version	NM_172430.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	A-kinase anchor protein SPHKAP
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY769819.1, BY774616.1, AK038638.1, AK122538.1, BC069832.1 and BC060165.1. On Mar 15, 2008 this sequence version replaced NM_172430.2. ##Evidence-Data-START## Transcript exon combination :: BC069832.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849377 [ECO:0000350] ##Evidence-Data-END##

ATGGATGTCA ACTCCCGGCT TTCTGTGCAA AGCAACGTGG AATCACCACT GATGCATGAA 
GGTTCAGAGC CACAGCAGAT CACCAGCAGT GCAGCAGGCA ACCTGGCTGG CTCCATCACG 
GCCTGTAAAA AGGTTCTTCG AAGCAATAGC TTGCTGGAGT CCACAGACTA CTGGCTGCAG 
AACCAGAGGA CGCCTTGCCA GATTGGGTTT GTGGAAGATG AGTCAGAAAA CTGTGCTTCT 
GTCTGCTTTG TGAACCTGGA TGTCAACAAA GATGCCTGCA TCACAGAGAA CCTGCAGCAG 
AAACTGGTCA ATGTTTCACC GGACCTTCCA AACCTCATCA GCTCCATGAA TGTCCAACAG 
CCGAAAGAAA ATGAAATTGT CCTCCTAAGT GGGTTAGCCT CTGGAAACCT CCAGGCAGAT 
TTTGATGTTT CACAGTGCCC TTGGCTACCA GATATCTGCT TGGTCCAGTG TGCAAGAGGG 
AACAGACCAA ACAGTACCAA CTGCATCATC TTTGAGATCA ACAAGTTCTT AATTGGTCTG 
GAAGTAGTGC AGGAGCGTCA GCTCCACCTG GAGACGAACG TGTTGAAGCT GGAGGATGAT 
ACAAACTGTT CTCTGTCTTC AATTGAAGAA GATTTTCTTA CAGCCTCCGA GCATTTGGAG 
GAAGAAATCG AGGTGGACGA CTGCAGAAGT GGTTTGGAAA ATACAAATGT GTCAGCTAAT 
GTTTTGGAAA GTAAAAAGCC AAAGGAAACT ACCCAGGAAG GATGGGATTA CCACAAGGAA 
AAGTTGCATT GTGCTTTAGG GGAAAAACAC ATTAGAAAAC ACCGTACGCC CTCCACAAAA 
ACAGAAGGGT CAAAGGAAAA TACAGAAGAG AACACGTCCT TGAAAAGTCT CAATCGTTTA 
GTCAGGCCAT CACACCTGAA AAGTGAAGTG GCGGGGAACA AGCAACTGGC CACAAACTAC 
TCCTACCCAG AAAATATCAA AGGGGAACTA GAAACATCTC AAATGCTGTT CATCCCTAGA 
GATGCCTATC TCTCCATGGT TAAGAAAGAT GTACTTTCTC CGTGTAGTGT ACTCAGTGAG 
CAGGGAGGCA GCCACAGAGA CCATGACGTT ACTCCAAACC CTCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA GCACCGGAGA GTATGCCACA AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC ACAGCCCACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
ATGCGAAGTG CTCTCCTTCC CAGTGGCTGT TGCACAAAAG ATATGGTGGT CCCTCGTTCA 
TGGAACGAGC TACCCAAAAT TGTCATTGTG CAGAGCCCAG ATGGCAGTGA CACAGTCCCT 
GAGCCAAATG TCTCCTCCTG GCCTGACATG GAATTTGTTG AAACCTCGGG CATCTTTTCT 
GCAGACAGTT CCAGCAGACC CACTCAGAGT GCCCTAGAAG TTGCATTGGC TTGTGCAGCC 
ACTGTGATTG GAACCATTTC CAGTCCACAG GCCACAGAAA GATTCGCCAT GGAGCAAGAA 
TCCCTGGTAT CTACCTATGC CCAGAGAGGC ACTGGGGTAC AGCAAACTCA AGTGCCCCAA 
GCATTCATGG CACCTTCTAC AACCGAGTAT TCCTTCCCAT CAGCTCTGTG TGGCATGACA 
CAAGTGGCAA GTGCTGTCGC TGTCTGTGGC TTATGTGAAA AAGAAGAGGC GACATGTCCT 
GTAGCTCCAA CTGACCTCTT GCCTACATCT GGGGCTTCTG AAGAAATATC CTCCATCGGA 
AGTTTAGTGA TGGAGAGGAG CACAGAACTG GGGAAGGAAG CCATTGCAGA GGCTCTGCTC 
AGAGAGGCTA CTCTGATTTT AGCAAGGCCA GATGCTTACA GCAGCCTTGG TGAGCTCTTG 
GAATCTGTGA ACCAGAGAAT CATAGAAACC ACTTCTAAAA CCCAGACCCT GTGCACAGAA 
AGTGTTCAAA GGAATGAACT GGCACACACC TTGTCCAATG TCATCCTCAA GCACTCCGTG 
GATGAACTTC ACCAGAAAAC TACAATGGCT CACCCCACTG ATGAAAGGCA TCCCTGTGGA 
ACTCTGGACA CCTTGATGGA AAGTGTGAAC CAGCTGCTGC ACAATGTGAT CTGCTTCACA 
TTCAAAAAGA TGAATCACAT TGTAACACTC AGTGAGCATC CCTCATTTGA TCAGGCTGCT 
GGTCAAGCCT GGGTAAAAGC CTTTGCATGC CCCAGCAGCC AGCCTCTTAG CAATGCACAC 
GGTACTGGCC TTGTCATCAG GAATCTTGTA GAGGATGCAT CTCCTAAGTC AAACAAGGGC 
GGAGCAAGGC CAGAACTGGT CAACAACCCC AGGTTGCAAT CTGAATTCTC CTGCAGTCAT 
AGAATGTTTG ATTCAACTGC TAAGTCATTC CCCAAGGAAA TATATCTGAA AGGGATTATG 
GGAGAGGATA CCAGGAACCC TCATCATACA CTAAATTATG ACAGCAATGA ACGAAGAGCC 
TCTACAGACC TAGGAAAATT GACAACAGCA AGCGAGGGTT GTAGTGGTTT CCAGGAAACT 
GAAGACAGCA TTGTTCCAAA CACCCAAGAG AAATACATCT GTGCCACACC CCTAAACAAT 
GAAGCTCAAG TTAACCTATC CTTATTAGGT GATGACCTGT CTGTTCCTGC TCAGTCTACT 
CTAGAGGCAA AGCAGTCCGA GGTCTATGGC ATCACAGATT TTGCGGAAGA ATTGGCAGAG 
ACTGTTGTCT CCATGGCAAC TGAAATTGCA GCAATCTGCC TTGACAACTC CAATGGCAAA 
CAGCCCTGGT TTTGTGCTTG GAAAAGAGGG AACGAGTTTT TAACGGCGCC TAACGGATCC 
TGCCGATCCT TGAAGAGGAA GAAGGAAAAC TCAAGCGCCG GAAGCACCGT GAGGAAACAC 
AAGCCACCTC GCCTCAGTGA AATCAAGAGA AAAGCTGATG AGCACCCAGA GCTGAAAGAG 
AAGCTGATGA ACAGAGTCAT GGATGAGTCC ATGAACCTCG AAGACATCCC TGATTCCGTC 
AGTACCTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TCACAGAGTT TTCCATGGTG 
GATGGGGTGT GGCAAGGCCA GAGTTGTTCC CGGACTCGGC TTCTGGGTGG CGATAGGTGG 
AACCGGCTGA AGGCCTCAAG CTGTGAGAGC ATTCCTGAGG AGGACTCTGA AGCCAGGGTC 
TTTGTAAACA GCCTGGGTTT GATGAGTACC TTAAGCCAAC CAGTTAGCAG GGCCAGCTCC 
GTCTCCAAAC AGTCCAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAA 
CAGATGGAAA ACGAAGGGAG AGGGTTTGAG TTGCTGCTGG ATTACTACGC AGGCAAAAAT 
GCCAGCAGTA TCATGAGCTC AGCGATGCAG CAGGCATGCC AGAAAAACGA CCACCTCAAC 
GTGAGACCGA GCTGCCCCTC TAAGCAATCC AGTACAGAGA GCATAACAGA GGAGTTCTAT 
AGGTACATGT TAAGGGACAT CGCGAAAGAA AGCAAAGATG GTGCCTCCTC CAGACGTAGC 
AGCCATGATT GGACCACGGG CCTGCTGTCT CCTTCTACAC GATCCCCTCT GTGTTACAGA 
CAGTCATCTA TGCCTGACAG CAGGTCGCCA TGCTCCAGAC TAACAGTAAA TGCGCCTGTC 
AAAGCCAACT CCTTAGATGG CTTTGCCCAA AACTGCCCTC AAGATTCTGT AAATGTACAG 
CCAGTCAGTA GGGCTTCCTC ATCTGGCCTC TGCAAATCAG ATTCCTGCTT GTATCGCAGA 
AGTGGGACTG ACCAGATCAC AAACATGCTA ATTCATGAAA CATGGGCCAG CTCCATTGAG 
GCTCTCATGA GAAAGAACAA AATCATTGCT GATGATAGCG AGGCAGCTAA TGCCAGTCCT 
GGCCCTGTTT CCAGTGGTTC TCCTTTGCAA GTAGAGAAGA ATGCCAACAG ATTAGCCACC 
AGCAAAGGAC ACAGGGGGCC AACTCTGCTT GTACAGGAAT CTGTTGATTA CCAGAGAAAA 
GATGCTGTTA CTGAAGGCAA TCATTCCCCA GTGTCATCTC CAGGCAAAAC AGCTCCTGTT 
AAAAAACCCA GTGATTTTGA TCCTAGAAGA GAAACCTCTG CATGCCACAA TGCTGCTGGT 
CTCAACAGCC CTAGGCGGTC ACTCTGCTCA AGGGATGTGC CTTTGATTCA GATAGAGACA 
GATCAGAAAG AAGAGTGCAT TGGAGAACCT GGACCTTTCC TTTCCCAAAG TGGCTCCCTA 
GAGGAAACAG AAGGCCACCA ACCTGAAGAA ACCATCCCAG ATGTGGCCAG AAATGAAGAC 
ACAGCTCCGA GCACCTGTCA GAGCTCTCGT GACAGCCTTG AGACCAGTGG GGAAGTAGAA 
GTGGAGGTCT TGAAAGAAGA CATACCCCGA GATGAGTCCC GGAACCCTCC TAGCAGCAGC 
GAGGAGAGTA CAGGCAGCTG GTCCCAGCTG GCCAATGAGG AGGACATCCC AGACGACACA 
AGCAGCTTTC TGCAGCTCAG CGAGCGGTCC ATGAGTGAAT TAGTAGAAGA AAAGGAGATT 
CTTAAAGAAC AATCAGAAAG CATAAAGGAA CATGCCTCTG GACTGCCAGG GAGAGCTGCC 
AGCCCCCAGA GGAGCCTACT GGTGATCAAC TTTGACCTGG AGCCAGAGTG TCCTGATGCT 
GAGCTTCGAG CCACTCTGCA GTGGATAGCT GCCTCCGAAC TGGGGATCCC AACAATCTAC 
TTTAAGAAAT CTCAGGAAAG CAGAATTGAA AAGTTTCTAG ATGTTGTGAA GCTTGTTCAG 
CAGAAGTCCT GGAAGGTGGG AGATATATTT CACGCGGTTG TCCAGTACTG CAAACTGCAT 
GCAGAGCAGA AGGAGAGGAC TCCGAGTCTC TTTGACTGGC TTCTGGAACT AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766018.3
CDS	190..5166
Translation	MDVNSRLSVQSNVESPLMHEGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASGNLQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETNVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETTQEGWDYHKEKLHCALGEKHIRKHRTPSTKTEGSKENTEENTSLKSLNRLVRPSHLKSEVAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKDVLSPCSVLSEQGGSHRDHDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSMRSALLPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADSSSRPTQSALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQAFMAPSTTEYSFPSALCGMTQVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSIGSLVMERSTELGKEAIAEALLREATLILARPDAYSSLGELLESVNQRIIETTSKTQTLCTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCGTLDTLMESVNQLLHNVICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLVEDASPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTLNYDSNERRASTDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLLGDDLSVPAQSTLEAKQSEVYGITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCSRTRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEFYRYMLRDIAKESKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVNAPVKANSLDGFAQNCPQDSVNVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQVEKNANRLATSKGHRGPTLLVQESVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAGLNSPRRSLCSRDVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSSRDSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLSERSMSELVEEKEILKEQSESIKEHASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG

Target ORF information:

RefSeq Version	NM_172430.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172430.3

ORF Insert Sequence:

ATGGATGTCA ACTCCCGGCT TTCTGTGCAA AGCAACGTGG AATCACCACT GATGCATGAA 
GGTTCAGAGC CACAGCAGAT CACCAGCAGT GCAGCAGGCA ACCTGGCTGG CTCCATCACG 
GCCTGTAAAA AGGTTCTTCG AAGCAATAGC TTGCTGGAGT CCACAGACTA CTGGCTGCAG 
AACCAGAGGA CGCCTTGCCA GATTGGGTTT GTGGAAGATG AGTCAGAAAA CTGTGCTTCT 
GTCTGCTTTG TGAACCTGGA TGTCAACAAA GATGCCTGCA TCACAGAGAA CCTGCAGCAG 
AAACTGGTCA ATGTTTCACC GGACCTTCCA AACCTCATCA GCTCCATGAA TGTCCAACAG 
CCGAAAGAAA ATGAAATTGT CCTCCTAAGT GGGTTAGCCT CTGGAAACCT CCAGGCAGAT 
TTTGATGTTT CACAGTGCCC TTGGCTACCA GATATCTGCT TGGTCCAGTG TGCAAGAGGG 
AACAGACCAA ACAGTACCAA CTGCATCATC TTTGAGATCA ACAAGTTCTT AATTGGTCTG 
GAAGTAGTGC AGGAGCGTCA GCTCCACCTG GAGACGAACG TGTTGAAGCT GGAGGATGAT 
ACAAACTGTT CTCTGTCTTC AATTGAAGAA GATTTTCTTA CAGCCTCCGA GCATTTGGAG 
GAAGAAATCG AGGTGGACGA CTGCAGAAGT GGTTTGGAAA ATACAAATGT GTCAGCTAAT 
GTTTTGGAAA GTAAAAAGCC AAAGGAAACT ACCCAGGAAG GATGGGATTA CCACAAGGAA 
AAGTTGCATT GTGCTTTAGG GGAAAAACAC ATTAGAAAAC ACCGTACGCC CTCCACAAAA 
ACAGAAGGGT CAAAGGAAAA TACAGAAGAG AACACGTCCT TGAAAAGTCT CAATCGTTTA 
GTCAGGCCAT CACACCTGAA AAGTGAAGTG GCGGGGAACA AGCAACTGGC CACAAACTAC 
TCCTACCCAG AAAATATCAA AGGGGAACTA GAAACATCTC AAATGCTGTT CATCCCTAGA 
GATGCCTATC TCTCCATGGT TAAGAAAGAT GTACTTTCTC CGTGTAGTGT ACTCAGTGAG 
CAGGGAGGCA GCCACAGAGA CCATGACGTT ACTCCAAACC CTCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA GCACCGGAGA GTATGCCACA AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC ACAGCCCACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
ATGCGAAGTG CTCTCCTTCC CAGTGGCTGT TGCACAAAAG ATATGGTGGT CCCTCGTTCA 
TGGAACGAGC TACCCAAAAT TGTCATTGTG CAGAGCCCAG ATGGCAGTGA CACAGTCCCT 
GAGCCAAATG TCTCCTCCTG GCCTGACATG GAATTTGTTG AAACCTCGGG CATCTTTTCT 
GCAGACAGTT CCAGCAGACC CACTCAGAGT GCCCTAGAAG TTGCATTGGC TTGTGCAGCC 
ACTGTGATTG GAACCATTTC CAGTCCACAG GCCACAGAAA GATTCGCCAT GGAGCAAGAA 
TCCCTGGTAT CTACCTATGC CCAGAGAGGC ACTGGGGTAC AGCAAACTCA AGTGCCCCAA 
GCATTCATGG CACCTTCTAC AACCGAGTAT TCCTTCCCAT CAGCTCTGTG TGGCATGACA 
CAAGTGGCAA GTGCTGTCGC TGTCTGTGGC TTATGTGAAA AAGAAGAGGC GACATGTCCT 
GTAGCTCCAA CTGACCTCTT GCCTACATCT GGGGCTTCTG AAGAAATATC CTCCATCGGA 
AGTTTAGTGA TGGAGAGGAG CACAGAACTG GGGAAGGAAG CCATTGCAGA GGCTCTGCTC 
AGAGAGGCTA CTCTGATTTT AGCAAGGCCA GATGCTTACA GCAGCCTTGG TGAGCTCTTG 
GAATCTGTGA ACCAGAGAAT CATAGAAACC ACTTCTAAAA CCCAGACCCT GTGCACAGAA 
AGTGTTCAAA GGAATGAACT GGCACACACC TTGTCCAATG TCATCCTCAA GCACTCCGTG 
GATGAACTTC ACCAGAAAAC TACAATGGCT CACCCCACTG ATGAAAGGCA TCCCTGTGGA 
ACTCTGGACA CCTTGATGGA AAGTGTGAAC CAGCTGCTGC ACAATGTGAT CTGCTTCACA 
TTCAAAAAGA TGAATCACAT TGTAACACTC AGTGAGCATC CCTCATTTGA TCAGGCTGCT 
GGTCAAGCCT GGGTAAAAGC CTTTGCATGC CCCAGCAGCC AGCCTCTTAG CAATGCACAC 
GGTACTGGCC TTGTCATCAG GAATCTTGTA GAGGATGCAT CTCCTAAGTC AAACAAGGGC 
GGAGCAAGGC CAGAACTGGT CAACAACCCC AGGTTGCAAT CTGAATTCTC CTGCAGTCAT 
AGAATGTTTG ATTCAACTGC TAAGTCATTC CCCAAGGAAA TATATCTGAA AGGGATTATG 
GGAGAGGATA CCAGGAACCC TCATCATACA CTAAATTATG ACAGCAATGA ACGAAGAGCC 
TCTACAGACC TAGGAAAATT GACAACAGCA AGCGAGGGTT GTAGTGGTTT CCAGGAAACT 
GAAGACAGCA TTGTTCCAAA CACCCAAGAG AAATACATCT GTGCCACACC CCTAAACAAT 
GAAGCTCAAG TTAACCTATC CTTATTAGGT GATGACCTGT CTGTTCCTGC TCAGTCTACT 
CTAGAGGCAA AGCAGTCCGA GGTCTATGGC ATCACAGATT TTGCGGAAGA ATTGGCAGAG 
ACTGTTGTCT CCATGGCAAC TGAAATTGCA GCAATCTGCC TTGACAACTC CAATGGCAAA 
CAGCCCTGGT TTTGTGCTTG GAAAAGAGGG AACGAGTTTT TAACGGCGCC TAACGGATCC 
TGCCGATCCT TGAAGAGGAA GAAGGAAAAC TCAAGCGCCG GAAGCACCGT GAGGAAACAC 
AAGCCACCTC GCCTCAGTGA AATCAAGAGA AAAGCTGATG AGCACCCAGA GCTGAAAGAG 
AAGCTGATGA ACAGAGTCAT GGATGAGTCC ATGAACCTCG AAGACATCCC TGATTCCGTC 
AGTACCTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TCACAGAGTT TTCCATGGTG 
GATGGGGTGT GGCAAGGCCA GAGTTGTTCC CGGACTCGGC TTCTGGGTGG CGATAGGTGG 
AACCGGCTGA AGGCCTCAAG CTGTGAGAGC ATTCCTGAGG AGGACTCTGA AGCCAGGGTC 
TTTGTAAACA GCCTGGGTTT GATGAGTACC TTAAGCCAAC CAGTTAGCAG GGCCAGCTCC 
GTCTCCAAAC AGTCCAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAA 
CAGATGGAAA ACGAAGGGAG AGGGTTTGAG TTGCTGCTGG ATTACTACGC AGGCAAAAAT 
GCCAGCAGTA TCATGAGCTC AGCGATGCAG CAGGCATGCC AGAAAAACGA CCACCTCAAC 
GTGAGACCGA GCTGCCCCTC TAAGCAATCC AGTACAGAGA GCATAACAGA GGAGTTCTAT 
AGGTACATGT TAAGGGACAT CGCGAAAGAA AGCAAAGATG GTGCCTCCTC CAGACGTAGC 
AGCCATGATT GGACCACGGG CCTGCTGTCT CCTTCTACAC GATCCCCTCT GTGTTACAGA 
CAGTCATCTA TGCCTGACAG CAGGTCGCCA TGCTCCAGAC TAACAGTAAA TGCGCCTGTC 
AAAGCCAACT CCTTAGATGG CTTTGCCCAA AACTGCCCTC AAGATTCTGT AAATGTACAG 
CCAGTCAGTA GGGCTTCCTC ATCTGGCCTC TGCAAATCAG ATTCCTGCTT GTATCGCAGA 
AGTGGGACTG ACCAGATCAC AAACATGCTA ATTCATGAAA CATGGGCCAG CTCCATTGAG 
GCTCTCATGA GAAAGAACAA AATCATTGCT GATGATAGCG AGGCAGCTAA TGCCAGTCCT 
GGCCCTGTTT CCAGTGGTTC TCCTTTGCAA GTAGAGAAGA ATGCCAACAG ATTAGCCACC 
AGCAAAGGAC ACAGGGGGCC AACTCTGCTT GTACAGGAAT CTGTTGATTA CCAGAGAAAA 
GATGCTGTTA CTGAAGGCAA TCATTCCCCA GTGTCATCTC CAGGCAAAAC AGCTCCTGTT 
AAAAAACCCA GTGATTTTGA TCCTAGAAGA GAAACCTCTG CATGCCACAA TGCTGCTGGT 
CTCAACAGCC CTAGGCGGTC ACTCTGCTCA AGGGATGTGC CTTTGATTCA GATAGAGACA 
GATCAGAAAG AAGAGTGCAT TGGAGAACCT GGACCTTTCC TTTCCCAAAG TGGCTCCCTA 
GAGGAAACAG AAGGCCACCA ACCTGAAGAA ACCATCCCAG ATGTGGCCAG AAATGAAGAC 
ACAGCTCCGA GCACCTGTCA GAGCTCTCGT GACAGCCTTG AGACCAGTGG GGAAGTAGAA 
GTGGAGGTCT TGAAAGAAGA CATACCCCGA GATGAGTCCC GGAACCCTCC TAGCAGCAGC 
GAGGAGAGTA CAGGCAGCTG GTCCCAGCTG GCCAATGAGG AGGACATCCC AGACGACACA 
AGCAGCTTTC TGCAGCTCAG CGAGCGGTCC ATGAGTGAAT TAGTAGAAGA AAAGGAGATT 
CTTAAAGAAC AATCAGAAAG CATAAAGGAA CATGCCTCTG GACTGCCAGG GAGAGCTGCC 
AGCCCCCAGA GGAGCCTACT GGTGATCAAC TTTGACCTGG AGCCAGAGTG TCCTGATGCT 
GAGCTTCGAG CCACTCTGCA GTGGATAGCT GCCTCCGAAC TGGGGATCCC AACAATCTAC 
TTTAAGAAAT CTCAGGAAAG CAGAATTGAA AAGTTTCTAG ATGTTGTGAA GCTTGTTCAG 
CAGAAGTCCT GGAAGGTGGG AGATATATTT CACGCGGTTG TCCAGTACTG CAAACTGCAT 
GCAGAGCAGA AGGAGAGGAC TCCGAGTCTC TTTGACTGGC TTCTGGAACT AGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( NM_001356547.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001343476.1 Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant 1, mRNA.

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CloneID	OMu27829
Clone ID Related Accession (Same CDS sequence)	XM_006496578.3 , NM_001356547.1
Accession Version	NM_001356547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	A-kinase anchor protein SPHKAP isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154144.9 and AC138359.8. On Oct 7, 2017 this sequence version replaced XM_006496578.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.109316.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849377, SAMN00849379 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCTGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC ATGAAGGTTC AGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAACCTG 
GCTGGCTCCA TCACGGCCTG TAAAAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGC TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGATGAGTCA 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAAGATGC CTGCATCACA 
GAGAACCTGC AGCAGAAACT GGTCAATGTT TCACCGGACC TTCCAAACCT CATCAGCTCC 
ATGAATGTCC AACAGCCGAA AGAAAATGAA ATTGTCCTCC TAAGTGGGTT AGCCTCTGGA 
AACCTCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGTGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT AGTGCAGGAG CGTCAGCTCC ACCTGGAGAC GAACGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCTCTG TCTTCAATTG AAGAAGATTT TCTTACAGCC 
TCCGAGCATT TGGAGGAAGA AATCGAGGTG GACGACTGCA GAAGTGGTTT GGAAAATACA 
AATGTGTCAG CTAATGTTTT GGAAAGTAAA AAGCCAAAGG AAACTACCCA GGAAGGATGG 
GATTACCACA AGGAAAAGTT GCATTGTGCT TTAGGGGAAA AACACATTAG AAAACACCGT 
ACGCCCTCCA CAAAAACAGA AGGGTCAAAG GAAAATACAG AAGAGAACAC GTCCTTGAAA 
AGTCTCAATC GTTTAGTCAG GCCATCACAC CTGAAAAGTG AAGTGGCGGG GAACAAGCAA 
CTGGCCACAA ACTACTCCTA CCCAGAAAAT ATCAAAGGGG AACTAGAAAC ATCTCAAATG 
CTGTTCATCC CTAGAGATGC CTATCTCTCC ATGGTTAAGA AAGATGTACT TTCTCCGTGT 
AGTGTACTCA GTGAGCAGGG AGGCAGCCAC AGAGACCATG ACGTTACTCC AAACCCTCTT 
CCTCCCGTTC AAAATGGAGA AGCCAGCACC GGAGAGTATG CCACAAACTT AGCAGAATCT 
GTAATGCAAG ATGCCTTCAT CCGATTGTCT CAGTCACAGC CCACACTCCC CCAGGAATCT 
GCAGTCAGTT TCTCTATGCG AAGTGCTCTC CTTCCCAGTG GCTGTTGCAC AAAAGATATG 
GTGGTCCCTC GTTCATGGAA CGAGCTACCC AAAATTGTCA TTGTGCAGAG CCCAGATGGC 
AGTGACACAG TCCCTGAGCC AAATGTCTCC TCCTGGCCTG ACATGGAATT TGTTGAAACC 
TCGGGCATCT TTTCTGCAGA CAGTTCCAGC AGACCCACTC AGAGTGCCCT AGAAGTTGCA 
TTGGCTTGTG CAGCCACTGT GATTGGAACC ATTTCCAGTC CACAGGCCAC AGAAAGATTC 
GCCATGGAGC AAGAATCCCT GGTATCTACC TATGCCCAGA GAGGCACTGG GGTACAGCAA 
ACTCAAGTGC CCCAAGCATT CATGGCACCT TCTACAACCG AGTATTCCTT CCCATCAGCT 
CTGTGTGGCA TGACACAAGT GGCAAGTGCT GTCGCTGTCT GTGGCTTATG TGAAAAAGAA 
GAGGCGACAT GTCCTGTAGC TCCAACTGAC CTCTTGCCTA CATCTGGGGC TTCTGAAGAA 
ATATCCTCCA TCGGAAGTTT AGTGATGGAG AGGAGCACAG AACTGGGGAA GGAAGCCATT 
GCAGAGGCTC TGCTCAGAGA GGCTACTCTG ATTTTAGCAA GGCCAGATGC TTACAGCAGC 
CTTGGTGAGC TCTTGGAATC TGTGAACCAG AGAATCATAG AAACCACTTC TAAAACCCAG 
ACCCTGTGCA CAGAAAGTGT TCAAAGGAAT GAACTGGCAC ACACCTTGTC CAATGTCATC 
CTCAAGCACT CCGTGGATGA ACTTCACCAG AAAACTACAA TGGCTCACCC CACTGATGAA 
AGGCATCCCT GTGGAACTCT GGACACCTTG ATGGAAAGTG TGAACCAGCT GCTGCACAAT 
GTGATCTGCT TCACATTCAA AAAGATGAAT CACATTGTAA CACTCAGTGA GCATCCCTCA 
TTTGATCAGG CTGCTGGTCA AGCCTGGGTA AAAGCCTTTG CATGCCCCAG CAGCCAGCCT 
CTTAGCAATG CACACGGTAC TGGCCTTGTC ATCAGGAATC TTGTAGAGGA TGCATCTCCT 
AAGTCAAACA AGGGCGGAGC AAGGCCAGAA CTGGTCAACA ACCCCAGGTT GCAATCTGAA 
TTCTCCTGCA GTCATAGAAT GTTTGATTCA ACTGCTAAGT CATTCCCCAA GGAAATATAT 
CTGAAAGGGA TTATGGGAGA GGATACCAGG AACCCTCATC ATACACTAAA TTATGACAGC 
AATGAACGAA GAGCCTCTAC AGACCTAGGA AAATTGACAA CAGCAAGCGA GGGTTGTAGT 
GGTTTCCAGG AAACTGAAGA CAGCATTGTT CCAAACACCC AAGAGAAATA CATCTGTGCC 
ACACCCCTAA ACAATGAAGC TCAAGTTAAC CTATCCTTAT TAGGTGATGA CCTGTCTGTT 
CCTGCTCAGT CTACTCTAGA GGCAAAGCAG TCCGAGGTCT ATGGCATCAC AGATTTTGCG 
GAAGAATTGG CAGAGACTGT TGTCTCCATG GCAACTGAAA TTGCAGCAAT CTGCCTTGAC 
AACTCCAATG GCAAACAGCC CTGGTTTTGT GCTTGGAAAA GAGGGAACGA GTTTTTAACG 
GCGCCTAACG GATCCTGCCG ATCCTTGAAG AGGAAGAAGG AAAACTCAAG CGCCGGAAGC 
ACCGTGAGGA AACACAAGCC ACCTCGCCTC AGTGAAATCA AGAGAAAAGC TGATGAGCAC 
CCAGAGCTGA AAGAGAAGCT GATGAACAGA GTCATGGATG AGTCCATGAA CCTCGAAGAC 
ATCCCTGATT CCGTCAGTAC CTTTGCCAAT GAAGTGGCAG CCAAGATCAT GAACCTCACA 
GAGTTTTCCA TGGTGGATGG GGTGTGGCAA GGCCAGAGTT GTTCCCGGAC TCGGCTTCTG 
GGTGGCGATA GGTGGAACCG GCTGAAGGCC TCAAGCTGTG AGAGCATTCC TGAGGAGGAC 
TCTGAAGCCA GGGTCTTTGT AAACAGCCTG GGTTTGATGA GTACCTTAAG CCAACCAGTT 
AGCAGGGCCA GCTCCGTCTC CAAACAGTCC AGCTGTGAGA GCATCACCGA TGAGTTTTCC 
AGGTTCATGG TGAAACAGAT GGAAAACGAA GGGAGAGGGT TTGAGTTGCT GCTGGATTAC 
TACGCAGGCA AAAATGCCAG CAGTATCATG AGCTCAGCGA TGCAGCAGGC ATGCCAGAAA 
AACGACCACC TCAACGTGAG ACCGAGCTGC CCCTCTAAGC AATCCAGTAC AGAGAGCATA 
ACAGAGGAGT TCTATAGGTA CATGTTAAGG GACATCGCGA AAGAAAGCAA AGATGGTGCC 
TCCTCCAGAC GTAGCAGCCA TGATTGGACC ACGGGCCTGC TGTCTCCTTC TACACGATCC 
CCTCTGTGTT ACAGACAGTC ATCTATGCCT GACAGCAGGT CGCCATGCTC CAGACTAACA 
GTAAATGCGC CTGTCAAAGC CAACTCCTTA GATGGCTTTG CCCAAAACTG CCCTCAAGAT 
TCTGTAAATG TACAGCCAGT CAGTAGGGCT TCCTCATCTG GCCTCTGCAA ATCAGATTCC 
TGCTTGTATC GCAGAAGTGG GACTGACCAG ATCACAAACA TGCTAATTCA TGAAACATGG 
GCCAGCTCCA TTGAGGCTCT CATGAGAAAG AACAAAATCA TTGCTGATGA TAGCGAGGCA 
GCTAATGCCA GTCCTGGCCC TGTTTCCAGT GGTTCTCCTT TGCAAGTAGA GAAGAATGCC 
AACAGATTAG CCACCAGCAA AGGACACAGG GGGCCAACTC TGCTTGTACA GGAATCTGTT 
GATTACCAGA GAAAAGATGC TGTTACTGAA GGCAATCATT CCCCAGTGTC ATCTCCAGGC 
AAAACAGCTC CTGTTAAAAA ACCCAGTGAT TTTGATCCTA GAAGAGAAAC CTCTGCATGC 
CACAATGCTG CTGGTCTCAA CAGCCCTAGG CGGTCACTCT GCTCAAGGGA TGTGCCTTTG 
ATTCAGATAG AGACAGATCA GAAAGAAGAG TGCATTGGAG AACCTGGACC TTTCCTTTCC 
CAAAGTGGCT CCCTAGAGGA AACAGAAGGC CACCAACCTG AAGAAACCAT CCCAGATGTG 
GCCAGAAATG AAGACACAGC TCCGAGCACC TGTCAGAGCT CTCGTGACAG CCTTGAGACC 
AGTGGGGAAG TAGAAGTGGA GGTCTTGAAA GAAGACATAC CCCGAGATGA GTCCCGGAAC 
CCTCCTAGCA GCAGCGAGGA GAGTACAGGC AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC 
ATCCCAGACG ACACAAGCAG CTTTCTGCAG CTCAGCGAGC GGTCCATGAG TGAATTAGTA 
GAAGAAAAGG AGATTCTTAA AGAACAATCA GAAAGCATAA AGGAACATGC CTCTGGACTG 
CCAGGGAGAG CTGCCAGCCC CCAGAGGAGC CTACTGGTGA TCAACTTTGA CCTGGAGCCA 
GAGTGTCCTG ATGCTGAGCT TCGAGCCACT CTGCAGTGGA TAGCTGCCTC CGAACTGGGG 
ATCCCAACAA TCTACTTTAA GAAATCTCAG GAAAGCAGAA TTGAAAAGTT TCTAGATGTT 
GTGAAGCTTG TTCAGCAGAA GTCCTGGAAG GTGGGAGATA TATTTCACGC GGTTGTCCAG 
TACTGCAAAC TGCATGCAGA GCAGAAGGAG AGGACTCCGA GTCTCTTTGA CTGGCTTCTG 
GAACTAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343476.1
CDS	194..5245
Translation	MSTPGFLCKAMWVVEPNSATCLSAALPVKRHCQESSNVESPLMHEGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASGNLQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETNVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETTQEGWDYHKEKLHCALGEKHIRKHRTPSTKTEGSKENTEENTSLKSLNRLVRPSHLKSEVAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKDVLSPCSVLSEQGGSHRDHDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSMRSALLPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADSSSRPTQSALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQAFMAPSTTEYSFPSALCGMTQVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSIGSLVMERSTELGKEAIAEALLREATLILARPDAYSSLGELLESVNQRIIETTSKTQTLCTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCGTLDTLMESVNQLLHNVICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLVEDASPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTLNYDSNERRASTDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLLGDDLSVPAQSTLEAKQSEVYGITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCSRTRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEFYRYMLRDIAKESKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVNAPVKANSLDGFAQNCPQDSVNVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQVEKNANRLATSKGHRGPTLLVQESVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAGLNSPRRSLCSRDVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSSRDSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLSERSMSELVEEKEILKEQSESIKEHASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG

Target ORF information:

RefSeq Version	NM_001356547.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356547.1

ORF Insert Sequence:

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCTGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC ATGAAGGTTC AGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAACCTG 
GCTGGCTCCA TCACGGCCTG TAAAAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGC TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGATGAGTCA 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAAGATGC CTGCATCACA 
GAGAACCTGC AGCAGAAACT GGTCAATGTT TCACCGGACC TTCCAAACCT CATCAGCTCC 
ATGAATGTCC AACAGCCGAA AGAAAATGAA ATTGTCCTCC TAAGTGGGTT AGCCTCTGGA 
AACCTCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGTGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT AGTGCAGGAG CGTCAGCTCC ACCTGGAGAC GAACGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCTCTG TCTTCAATTG AAGAAGATTT TCTTACAGCC 
TCCGAGCATT TGGAGGAAGA AATCGAGGTG GACGACTGCA GAAGTGGTTT GGAAAATACA 
AATGTGTCAG CTAATGTTTT GGAAAGTAAA AAGCCAAAGG AAACTACCCA GGAAGGATGG 
GATTACCACA AGGAAAAGTT GCATTGTGCT TTAGGGGAAA AACACATTAG AAAACACCGT 
ACGCCCTCCA CAAAAACAGA AGGGTCAAAG GAAAATACAG AAGAGAACAC GTCCTTGAAA 
AGTCTCAATC GTTTAGTCAG GCCATCACAC CTGAAAAGTG AAGTGGCGGG GAACAAGCAA 
CTGGCCACAA ACTACTCCTA CCCAGAAAAT ATCAAAGGGG AACTAGAAAC ATCTCAAATG 
CTGTTCATCC CTAGAGATGC CTATCTCTCC ATGGTTAAGA AAGATGTACT TTCTCCGTGT 
AGTGTACTCA GTGAGCAGGG AGGCAGCCAC AGAGACCATG ACGTTACTCC AAACCCTCTT 
CCTCCCGTTC AAAATGGAGA AGCCAGCACC GGAGAGTATG CCACAAACTT AGCAGAATCT 
GTAATGCAAG ATGCCTTCAT CCGATTGTCT CAGTCACAGC CCACACTCCC CCAGGAATCT 
GCAGTCAGTT TCTCTATGCG AAGTGCTCTC CTTCCCAGTG GCTGTTGCAC AAAAGATATG 
GTGGTCCCTC GTTCATGGAA CGAGCTACCC AAAATTGTCA TTGTGCAGAG CCCAGATGGC 
AGTGACACAG TCCCTGAGCC AAATGTCTCC TCCTGGCCTG ACATGGAATT TGTTGAAACC 
TCGGGCATCT TTTCTGCAGA CAGTTCCAGC AGACCCACTC AGAGTGCCCT AGAAGTTGCA 
TTGGCTTGTG CAGCCACTGT GATTGGAACC ATTTCCAGTC CACAGGCCAC AGAAAGATTC 
GCCATGGAGC AAGAATCCCT GGTATCTACC TATGCCCAGA GAGGCACTGG GGTACAGCAA 
ACTCAAGTGC CCCAAGCATT CATGGCACCT TCTACAACCG AGTATTCCTT CCCATCAGCT 
CTGTGTGGCA TGACACAAGT GGCAAGTGCT GTCGCTGTCT GTGGCTTATG TGAAAAAGAA 
GAGGCGACAT GTCCTGTAGC TCCAACTGAC CTCTTGCCTA CATCTGGGGC TTCTGAAGAA 
ATATCCTCCA TCGGAAGTTT AGTGATGGAG AGGAGCACAG AACTGGGGAA GGAAGCCATT 
GCAGAGGCTC TGCTCAGAGA GGCTACTCTG ATTTTAGCAA GGCCAGATGC TTACAGCAGC 
CTTGGTGAGC TCTTGGAATC TGTGAACCAG AGAATCATAG AAACCACTTC TAAAACCCAG 
ACCCTGTGCA CAGAAAGTGT TCAAAGGAAT GAACTGGCAC ACACCTTGTC CAATGTCATC 
CTCAAGCACT CCGTGGATGA ACTTCACCAG AAAACTACAA TGGCTCACCC CACTGATGAA 
AGGCATCCCT GTGGAACTCT GGACACCTTG ATGGAAAGTG TGAACCAGCT GCTGCACAAT 
GTGATCTGCT TCACATTCAA AAAGATGAAT CACATTGTAA CACTCAGTGA GCATCCCTCA 
TTTGATCAGG CTGCTGGTCA AGCCTGGGTA AAAGCCTTTG CATGCCCCAG CAGCCAGCCT 
CTTAGCAATG CACACGGTAC TGGCCTTGTC ATCAGGAATC TTGTAGAGGA TGCATCTCCT 
AAGTCAAACA AGGGCGGAGC AAGGCCAGAA CTGGTCAACA ACCCCAGGTT GCAATCTGAA 
TTCTCCTGCA GTCATAGAAT GTTTGATTCA ACTGCTAAGT CATTCCCCAA GGAAATATAT 
CTGAAAGGGA TTATGGGAGA GGATACCAGG AACCCTCATC ATACACTAAA TTATGACAGC 
AATGAACGAA GAGCCTCTAC AGACCTAGGA AAATTGACAA CAGCAAGCGA GGGTTGTAGT 
GGTTTCCAGG AAACTGAAGA CAGCATTGTT CCAAACACCC AAGAGAAATA CATCTGTGCC 
ACACCCCTAA ACAATGAAGC TCAAGTTAAC CTATCCTTAT TAGGTGATGA CCTGTCTGTT 
CCTGCTCAGT CTACTCTAGA GGCAAAGCAG TCCGAGGTCT ATGGCATCAC AGATTTTGCG 
GAAGAATTGG CAGAGACTGT TGTCTCCATG GCAACTGAAA TTGCAGCAAT CTGCCTTGAC 
AACTCCAATG GCAAACAGCC CTGGTTTTGT GCTTGGAAAA GAGGGAACGA GTTTTTAACG 
GCGCCTAACG GATCCTGCCG ATCCTTGAAG AGGAAGAAGG AAAACTCAAG CGCCGGAAGC 
ACCGTGAGGA AACACAAGCC ACCTCGCCTC AGTGAAATCA AGAGAAAAGC TGATGAGCAC 
CCAGAGCTGA AAGAGAAGCT GATGAACAGA GTCATGGATG AGTCCATGAA CCTCGAAGAC 
ATCCCTGATT CCGTCAGTAC CTTTGCCAAT GAAGTGGCAG CCAAGATCAT GAACCTCACA 
GAGTTTTCCA TGGTGGATGG GGTGTGGCAA GGCCAGAGTT GTTCCCGGAC TCGGCTTCTG 
GGTGGCGATA GGTGGAACCG GCTGAAGGCC TCAAGCTGTG AGAGCATTCC TGAGGAGGAC 
TCTGAAGCCA GGGTCTTTGT AAACAGCCTG GGTTTGATGA GTACCTTAAG CCAACCAGTT 
AGCAGGGCCA GCTCCGTCTC CAAACAGTCC AGCTGTGAGA GCATCACCGA TGAGTTTTCC 
AGGTTCATGG TGAAACAGAT GGAAAACGAA GGGAGAGGGT TTGAGTTGCT GCTGGATTAC 
TACGCAGGCA AAAATGCCAG CAGTATCATG AGCTCAGCGA TGCAGCAGGC ATGCCAGAAA 
AACGACCACC TCAACGTGAG ACCGAGCTGC CCCTCTAAGC AATCCAGTAC AGAGAGCATA 
ACAGAGGAGT TCTATAGGTA CATGTTAAGG GACATCGCGA AAGAAAGCAA AGATGGTGCC 
TCCTCCAGAC GTAGCAGCCA TGATTGGACC ACGGGCCTGC TGTCTCCTTC TACACGATCC 
CCTCTGTGTT ACAGACAGTC ATCTATGCCT GACAGCAGGT CGCCATGCTC CAGACTAACA 
GTAAATGCGC CTGTCAAAGC CAACTCCTTA GATGGCTTTG CCCAAAACTG CCCTCAAGAT 
TCTGTAAATG TACAGCCAGT CAGTAGGGCT TCCTCATCTG GCCTCTGCAA ATCAGATTCC 
TGCTTGTATC GCAGAAGTGG GACTGACCAG ATCACAAACA TGCTAATTCA TGAAACATGG 
GCCAGCTCCA TTGAGGCTCT CATGAGAAAG AACAAAATCA TTGCTGATGA TAGCGAGGCA 
GCTAATGCCA GTCCTGGCCC TGTTTCCAGT GGTTCTCCTT TGCAAGTAGA GAAGAATGCC 
AACAGATTAG CCACCAGCAA AGGACACAGG GGGCCAACTC TGCTTGTACA GGAATCTGTT 
GATTACCAGA GAAAAGATGC TGTTACTGAA GGCAATCATT CCCCAGTGTC ATCTCCAGGC 
AAAACAGCTC CTGTTAAAAA ACCCAGTGAT TTTGATCCTA GAAGAGAAAC CTCTGCATGC 
CACAATGCTG CTGGTCTCAA CAGCCCTAGG CGGTCACTCT GCTCAAGGGA TGTGCCTTTG 
ATTCAGATAG AGACAGATCA GAAAGAAGAG TGCATTGGAG AACCTGGACC TTTCCTTTCC 
CAAAGTGGCT CCCTAGAGGA AACAGAAGGC CACCAACCTG AAGAAACCAT CCCAGATGTG 
GCCAGAAATG AAGACACAGC TCCGAGCACC TGTCAGAGCT CTCGTGACAG CCTTGAGACC 
AGTGGGGAAG TAGAAGTGGA GGTCTTGAAA GAAGACATAC CCCGAGATGA GTCCCGGAAC 
CCTCCTAGCA GCAGCGAGGA GAGTACAGGC AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC 
ATCCCAGACG ACACAAGCAG CTTTCTGCAG CTCAGCGAGC GGTCCATGAG TGAATTAGTA 
GAAGAAAAGG AGATTCTTAA AGAACAATCA GAAAGCATAA AGGAACATGC CTCTGGACTG 
CCAGGGAGAG CTGCCAGCCC CCAGAGGAGC CTACTGGTGA TCAACTTTGA CCTGGAGCCA 
GAGTGTCCTG ATGCTGAGCT TCGAGCCACT CTGCAGTGGA TAGCTGCCTC CGAACTGGGG 
ATCCCAACAA TCTACTTTAA GAAATCTCAG GAAAGCAGAA TTGAAAAGTT TCTAGATGTT 
GTGAAGCTTG TTCAGCAGAA GTCCTGGAAG GTGGGAGATA TATTTCACGC GGTTGTCCAG 
TACTGCAAAC TGCATGCAGA GCAGAAGGAG AGGACTCCGA GTCTCTTTGA CTGGCTTCTG 
GAACTAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( XM_006496575.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496638.1 Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X1, mRNA.

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CloneID	OMu27826
Clone ID Related Accession (Same CDS sequence)	XM_006496575.3 , XM_006496575.4
Accession Version	XM_006496575.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	A-kinase anchor protein SPHKAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496575.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCTGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC ATGAAGGTTC AGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAACCTG 
GCTGGCTCCA TCACGGCCTG TAAAAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGC TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGATGAGTCA 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAAGATGC CTGCATCACA 
GAGAACCTGC AGCAGAAACT GGTCAATGTT TCACCGGACC TTCCAAACCT CATCAGCTCC 
ATGAATGTCC AACAGCCGAA AGAAAATGAA ATTGTCCTCC TAAGTGGGTT AGCCTCTGGA 
AACCTCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGTGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT AGTGCAGGAG CGTCAGCTCC ACCTGGAGAC GAACGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCTCTG TCTTCAATTG AAGAAGATTT TCTTACAGCC 
TCCGAGCATT TGGAGGAAGA AATCGAGGTG GACGACTGCA GAAGTGGTTT GGAAAATACA 
AATGTGTCAG CTAATGTTTT GGAAAGTAAA AAGCCAAAGG AAACTACCCA GGAAGGATGG 
GATTACCACA AGGAAAAGTT GCATTGTGCT TTAGGGGAAA AACACATTAG AAAACACCGT 
ACGCCCTCCA CAAAAACAGA AGGGTCAAAG GAAAATACAG AAGAGAACAC GTCCTTGAAA 
AGTCTCAATC GTTTAGTCAG GCCATCACAC CTGAAAAGTG AAGTGGCGGG GAACAAGCAA 
CTGGCCACAA ACTACTCCTA CCCAGAAAAT ATCAAAGGGG AACTAGAAAC ATCTCAAATG 
CTGTTCATCC CTAGAGATGC CTATCTCTCC ATGGTTAAGA AAGATGTACT TTCTCCGTGT 
AGTGTACTCA GTGAGCAGGG AGGCAGCCAC AGAGACCATG ACGTTACTCC AAACCCTCTT 
CCTCCCGTTC AAAATGGAGA AGCCAGCACC GGAGAGTATG CCACAAACTT AGCAGAATCT 
GTAATGCAAG ATGCCTTCAT CCGATTGTCT CAGTCACAGC CCACACTCCC CCAGGAATCT 
GCAGTCAGTT TCTCTATGCG AAGTGCTCTC CTTCCCAGTG GCTGTTGCAC AAAAGATATG 
GTGGTCCCTC GTTCATGGAA CGAGCTACCC AAAATTGTCA TTGTGCAGAG CCCAGATGGC 
AGTGACACAG TCCCTGAGCC AAATGTCTCC TCCTGGCCTG ACATGGAATT TGTTGAAACC 
TCGGGCATCT TTTCTGCAGA CAGTTCCAGC AGACCCACTC AGAGTGCCCT AGAAGTTGCA 
TTGGCTTGTG CAGCCACTGT GATTGGAACC ATTTCCAGTC CACAGGCCAC AGAAAGATTC 
GCCATGGAGC AAGAATCCCT GGTATCTACC TATGCCCAGA GAGGCACTGG GGTACAGCAA 
ACTCAAGTGC CCCAAGCATT CATGGCACCT TCTACAACCG AGTATTCCTT CCCATCAGCT 
CTGTGTGGCA TGACACAAGT GGCAAGTGCT GTCGCTGTCT GTGGCTTATG TGAAAAAGAA 
GAGGCGACAT GTCCTGTAGC TCCAACTGAC CTCTTGCCTA CATCTGGGGC TTCTGAAGAA 
ATATCCTCCA TCGGAAGTTT AGTGATGGAG AGGAGCACAG AACTGGGGAA GGAAGCCATT 
GCAGAGGCTC TGCTCAGAGA GGCTACTCTG ATTTTAGCAA GGCCAGATGC TTACAGCAGC 
CTTGGTGAGC TCTTGGAATC TGTGAACCAG AGAATCATAG AAACCACTTC TAAAACCCAG 
ACCCTGTGCA CAGAAAGTGT TCAAAGGAAT GAACTGGCAC ACACCTTGTC CAATGTCATC 
CTCAAGCACT CCGTGGATGA ACTTCACCAG AAAACTACAA TGGCTCACCC CACTGATGAA 
AGGCATCCCT GTGGAACTCT GGACACCTTG ATGGAAAGTG TGAACCAGCT GCTGCACAAT 
GTGATCTGCT TCACATTCAA AAAGATGAAT CACATTGTAA CACTCAGTGA GCATCCCTCA 
TTTGATCAGG CTGCTGGTCA AGCCTGGGTA AAAGCCTTTG CATGCCCCAG CAGCCAGCCT 
CTTAGCAATG CACACGGTAC TGGCCTTGTC ATCAGGAATC TTGTAGAGGA TGCATCTCCT 
AAGTCAAACA AGGGCGGAGC AAGGCCAGAA CTGGTCAACA ACCCCAGGTT GCAATCTGAA 
TTCTCCTGCA GTCATAGAAT GTTTGATTCA ACTGCTAAGT CATTCCCCAA GGAAATATAT 
CTGAAAGGGA TTATGGGAGA GGATACCAGG AACCCTCATC ATACACTAAA TTATGACAGC 
AATGAACGAA GAGCCTCTAC AGACCTAGGA AAATTGACAA CAGCAAGCGA GGGTTGTAGT 
GGTTTCCAGG AAACTGAAGA CAGCATTGTT CCAAACACCC AAGAGAAATA CATCTGTGCC 
ACACCCCTAA ACAATGAAGC TCAAGTTAAC CTATCCTTAT TAGGTGATGA CCTGTCTGTT 
CCTGCTCAGT CTACTCTAGA GGCAAAGCAG TCCGAGGTCT ATGGCATCAC AGATTTTGCG 
GAAGAATTGG CAGAGACTGT TGTCTCCATG GCAACTGAAA TTGCAGCAAT CTGCCTTGAC 
AACTCCAATG GCAAACAGCC CTGGTTTTGT GCTTGGAAAA GAGGGAACGA GTTTTTAACG 
GCGCCTAACG GATCCTGCCG ATCCTTGAAG AGGAAGAAGG AAAACTCAAG CGCCGGAAGC 
ACCGTGAGGA AACACAAGCC ACCTCGCCTC AGTGAAATCA AGAGAAAAGC TGATGAGCAC 
CCAGAGCTGA AAGAGAAGCT GATGAACAGA GTCATGGATG AGTCCATGAA CCTCGAAGAC 
ATCCCTGATT CCGTCAGTAC CTTTGCCAAT GAAGTGGCAG CCAAGATCAT GAACCTCACA 
GAGTTTTCCA TGGTGGATGG GGTGTGGCAA GGCCAGAGTT GTTCCCGGAC TCGGCTTCTG 
GGTGGCGATA GGTGGAACCG GCTGAAGGCC TCAAGCTGTG AGAGCATTCC TGAGGAGGAC 
TCTGAAGCCA GGGTCTTTGT AAACAGCCTG GGTTTGATGA GTACCTTAAG CCAACCAGTT 
AGCAGGGCCA GCTCCGTCTC CAAACAGTCC AGCTGTGAGA GCATCACCGA TGAGTTTTCC 
AGGTTCATGG TGAAACAGAT GGAAAACGAA GGGAGAGGGT TTGAGTTGCT GCTGGATTAC 
TACGCAGGCA AAAATGCCAG CAGTATCATG AGCTCAGCGA TGCAGCAGGC ATGCCAGAAA 
AACGACCACC TCAACGTGAG ACCGAGCTGC CCCTCTAAGC AATCCAGTAC AGAGAGCATA 
ACAGAGGAGT TCTATAGGTA CATGTTAAGG GACATCGCGA AAGAAAGCAA AGATGGTGCC 
TCCTCCAGAC GTAGCAGCCA TGATTGGACC ACGGGCCTGC TGTCTCCTTC TACACGATCC 
CCTCTGTGTT ACAGACAGTC ATCTATGCCT GACAGCAGGT CGCCATGCTC CAGACTAACA 
GTAAATGCGC CTGTCAAAGC CAACTCCTTA GATGGCTTTG CCCAAAACTG CCCTCAAGAT 
TCTGTAAATG TACAGCCAGT CAGTAGGGCT TCCTCATCTG GCCTCTGCAA ATCAGATTCC 
TGCTTGTATC GCAGAAGTGG GACTGACCAG ATCACAAACA TGCTAATTCA TGAAACATGG 
GCCAGCTCCA TTGAGGCTCT CATGAGAAAG AACAAAATCA TTGCTGATGA TAGCGAGGCA 
GCTAATGCCA GTCCTGGCCC TGTTTCCAGT GGTTCTCCTT TGCAAGTAGA GAAGAATGCC 
AACAGATTAG CCACCAGCAA AGGACACAGG GGGCCAACTC TGCTTGTACA GGAATCTGTT 
GATTACCAGA GAAAAGATGC TGTTACTGAA GGCAATCATT CCCCAGTGTC ATCTCCAGGC 
AAAACAGCTC CTGTTAAAAA ACCCAGTGAT TTTGATCCTA GAAGAGAAAC CTCTGCATGC 
CACAATGCTG CTGGTCTCAA CAGCCCTAGG CGGTCACTCT GCTCAAGGGA TGTGCCTTTG 
ATTCAGATAG AGACAGATCA GAAAGAAGAG TGCATTGGAG AACCTGGACC TTTCCTTTCC 
CAAAGTGGCT CCCTAGAGGA AACAGAAGGC CACCAACCTG AAGAAACCAT CCCAGATGTG 
GCCAGAAATG AAGACACAGC TCCGAGCACC TGTCAGAGCT CTCGTGACAG CCTTGAGACC 
AGTGGGGAAG TAGAAGTGGA GGTCTTGAAA GAAGACATAC CCCGAGATGA GTCCCGGAAC 
CCTCCTAGCA GCAGCGAGGA GAGTACAGGC AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC 
ATCCCAGACG ACACAAGCAG CTTTCTGCAG CTCAGCGAGC GGTCCATGAG CAATGGCAAC 
AGTAGTGGCA CTAGCAGTCT TGGCATTATG GACCTGGACA TTTATCAGGA AAGCATACCG 
TCTTCTCCCA TGATTAATGA ATTAGTAGAA GAAAAGGAGA TTCTTAAAGA ACAATCAGAA 
AGCATAAAGG AACATGCCTC TGGACTGCCA GGGAGAGCTG CCAGCCCCCA GAGGAGCCTA 
CTGGTGATCA ACTTTGACCT GGAGCCAGAG TGTCCTGATG CTGAGCTTCG AGCCACTCTG 
CAGTGGATAG CTGCCTCCGA ACTGGGGATC CCAACAATCT ACTTTAAGAA ATCTCAGGAA 
AGCAGAATTG AAAAGTTTCT AGATGTTGTG AAGCTTGTTC AGCAGAAGTC CTGGAAGGTG 
GGAGATATAT TTCACGCGGT TGTCCAGTAC TGCAAACTGC ATGCAGAGCA GAAGGAGAGG 
ACTCCGAGTC TCTTTGACTG GCTTCTGGAA CTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496638.1
CDS	386..5524
Translation	MSTPGFLCKAMWVVEPNSATCLSAALPVKRHCQESSNVESPLMHEGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASGNLQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETNVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETTQEGWDYHKEKLHCALGEKHIRKHRTPSTKTEGSKENTEENTSLKSLNRLVRPSHLKSEVAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKDVLSPCSVLSEQGGSHRDHDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSMRSALLPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADSSSRPTQSALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQAFMAPSTTEYSFPSALCGMTQVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSIGSLVMERSTELGKEAIAEALLREATLILARPDAYSSLGELLESVNQRIIETTSKTQTLCTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCGTLDTLMESVNQLLHNVICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLVEDASPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTLNYDSNERRASTDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLLGDDLSVPAQSTLEAKQSEVYGITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCSRTRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEFYRYMLRDIAKESKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVNAPVKANSLDGFAQNCPQDSVNVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQVEKNANRLATSKGHRGPTLLVQESVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAGLNSPRRSLCSRDVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSSRDSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLSERSMSNGNSSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESIKEHASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006496575.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496575.3

ORF Insert Sequence:

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCTGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC ATGAAGGTTC AGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAACCTG 
GCTGGCTCCA TCACGGCCTG TAAAAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGC TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGATGAGTCA 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAAGATGC CTGCATCACA 
GAGAACCTGC AGCAGAAACT GGTCAATGTT TCACCGGACC TTCCAAACCT CATCAGCTCC 
ATGAATGTCC AACAGCCGAA AGAAAATGAA ATTGTCCTCC TAAGTGGGTT AGCCTCTGGA 
AACCTCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGTGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT AGTGCAGGAG CGTCAGCTCC ACCTGGAGAC GAACGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCTCTG TCTTCAATTG AAGAAGATTT TCTTACAGCC 
TCCGAGCATT TGGAGGAAGA AATCGAGGTG GACGACTGCA GAAGTGGTTT GGAAAATACA 
AATGTGTCAG CTAATGTTTT GGAAAGTAAA AAGCCAAAGG AAACTACCCA GGAAGGATGG 
GATTACCACA AGGAAAAGTT GCATTGTGCT TTAGGGGAAA AACACATTAG AAAACACCGT 
ACGCCCTCCA CAAAAACAGA AGGGTCAAAG GAAAATACAG AAGAGAACAC GTCCTTGAAA 
AGTCTCAATC GTTTAGTCAG GCCATCACAC CTGAAAAGTG AAGTGGCGGG GAACAAGCAA 
CTGGCCACAA ACTACTCCTA CCCAGAAAAT ATCAAAGGGG AACTAGAAAC ATCTCAAATG 
CTGTTCATCC CTAGAGATGC CTATCTCTCC ATGGTTAAGA AAGATGTACT TTCTCCGTGT 
AGTGTACTCA GTGAGCAGGG AGGCAGCCAC AGAGACCATG ACGTTACTCC AAACCCTCTT 
CCTCCCGTTC AAAATGGAGA AGCCAGCACC GGAGAGTATG CCACAAACTT AGCAGAATCT 
GTAATGCAAG ATGCCTTCAT CCGATTGTCT CAGTCACAGC CCACACTCCC CCAGGAATCT 
GCAGTCAGTT TCTCTATGCG AAGTGCTCTC CTTCCCAGTG GCTGTTGCAC AAAAGATATG 
GTGGTCCCTC GTTCATGGAA CGAGCTACCC AAAATTGTCA TTGTGCAGAG CCCAGATGGC 
AGTGACACAG TCCCTGAGCC AAATGTCTCC TCCTGGCCTG ACATGGAATT TGTTGAAACC 
TCGGGCATCT TTTCTGCAGA CAGTTCCAGC AGACCCACTC AGAGTGCCCT AGAAGTTGCA 
TTGGCTTGTG CAGCCACTGT GATTGGAACC ATTTCCAGTC CACAGGCCAC AGAAAGATTC 
GCCATGGAGC AAGAATCCCT GGTATCTACC TATGCCCAGA GAGGCACTGG GGTACAGCAA 
ACTCAAGTGC CCCAAGCATT CATGGCACCT TCTACAACCG AGTATTCCTT CCCATCAGCT 
CTGTGTGGCA TGACACAAGT GGCAAGTGCT GTCGCTGTCT GTGGCTTATG TGAAAAAGAA 
GAGGCGACAT GTCCTGTAGC TCCAACTGAC CTCTTGCCTA CATCTGGGGC TTCTGAAGAA 
ATATCCTCCA TCGGAAGTTT AGTGATGGAG AGGAGCACAG AACTGGGGAA GGAAGCCATT 
GCAGAGGCTC TGCTCAGAGA GGCTACTCTG ATTTTAGCAA GGCCAGATGC TTACAGCAGC 
CTTGGTGAGC TCTTGGAATC TGTGAACCAG AGAATCATAG AAACCACTTC TAAAACCCAG 
ACCCTGTGCA CAGAAAGTGT TCAAAGGAAT GAACTGGCAC ACACCTTGTC CAATGTCATC 
CTCAAGCACT CCGTGGATGA ACTTCACCAG AAAACTACAA TGGCTCACCC CACTGATGAA 
AGGCATCCCT GTGGAACTCT GGACACCTTG ATGGAAAGTG TGAACCAGCT GCTGCACAAT 
GTGATCTGCT TCACATTCAA AAAGATGAAT CACATTGTAA CACTCAGTGA GCATCCCTCA 
TTTGATCAGG CTGCTGGTCA AGCCTGGGTA AAAGCCTTTG CATGCCCCAG CAGCCAGCCT 
CTTAGCAATG CACACGGTAC TGGCCTTGTC ATCAGGAATC TTGTAGAGGA TGCATCTCCT 
AAGTCAAACA AGGGCGGAGC AAGGCCAGAA CTGGTCAACA ACCCCAGGTT GCAATCTGAA 
TTCTCCTGCA GTCATAGAAT GTTTGATTCA ACTGCTAAGT CATTCCCCAA GGAAATATAT 
CTGAAAGGGA TTATGGGAGA GGATACCAGG AACCCTCATC ATACACTAAA TTATGACAGC 
AATGAACGAA GAGCCTCTAC AGACCTAGGA AAATTGACAA CAGCAAGCGA GGGTTGTAGT 
GGTTTCCAGG AAACTGAAGA CAGCATTGTT CCAAACACCC AAGAGAAATA CATCTGTGCC 
ACACCCCTAA ACAATGAAGC TCAAGTTAAC CTATCCTTAT TAGGTGATGA CCTGTCTGTT 
CCTGCTCAGT CTACTCTAGA GGCAAAGCAG TCCGAGGTCT ATGGCATCAC AGATTTTGCG 
GAAGAATTGG CAGAGACTGT TGTCTCCATG GCAACTGAAA TTGCAGCAAT CTGCCTTGAC 
AACTCCAATG GCAAACAGCC CTGGTTTTGT GCTTGGAAAA GAGGGAACGA GTTTTTAACG 
GCGCCTAACG GATCCTGCCG ATCCTTGAAG AGGAAGAAGG AAAACTCAAG CGCCGGAAGC 
ACCGTGAGGA AACACAAGCC ACCTCGCCTC AGTGAAATCA AGAGAAAAGC TGATGAGCAC 
CCAGAGCTGA AAGAGAAGCT GATGAACAGA GTCATGGATG AGTCCATGAA CCTCGAAGAC 
ATCCCTGATT CCGTCAGTAC CTTTGCCAAT GAAGTGGCAG CCAAGATCAT GAACCTCACA 
GAGTTTTCCA TGGTGGATGG GGTGTGGCAA GGCCAGAGTT GTTCCCGGAC TCGGCTTCTG 
GGTGGCGATA GGTGGAACCG GCTGAAGGCC TCAAGCTGTG AGAGCATTCC TGAGGAGGAC 
TCTGAAGCCA GGGTCTTTGT AAACAGCCTG GGTTTGATGA GTACCTTAAG CCAACCAGTT 
AGCAGGGCCA GCTCCGTCTC CAAACAGTCC AGCTGTGAGA GCATCACCGA TGAGTTTTCC 
AGGTTCATGG TGAAACAGAT GGAAAACGAA GGGAGAGGGT TTGAGTTGCT GCTGGATTAC 
TACGCAGGCA AAAATGCCAG CAGTATCATG AGCTCAGCGA TGCAGCAGGC ATGCCAGAAA 
AACGACCACC TCAACGTGAG ACCGAGCTGC CCCTCTAAGC AATCCAGTAC AGAGAGCATA 
ACAGAGGAGT TCTATAGGTA CATGTTAAGG GACATCGCGA AAGAAAGCAA AGATGGTGCC 
TCCTCCAGAC GTAGCAGCCA TGATTGGACC ACGGGCCTGC TGTCTCCTTC TACACGATCC 
CCTCTGTGTT ACAGACAGTC ATCTATGCCT GACAGCAGGT CGCCATGCTC CAGACTAACA 
GTAAATGCGC CTGTCAAAGC CAACTCCTTA GATGGCTTTG CCCAAAACTG CCCTCAAGAT 
TCTGTAAATG TACAGCCAGT CAGTAGGGCT TCCTCATCTG GCCTCTGCAA ATCAGATTCC 
TGCTTGTATC GCAGAAGTGG GACTGACCAG ATCACAAACA TGCTAATTCA TGAAACATGG 
GCCAGCTCCA TTGAGGCTCT CATGAGAAAG AACAAAATCA TTGCTGATGA TAGCGAGGCA 
GCTAATGCCA GTCCTGGCCC TGTTTCCAGT GGTTCTCCTT TGCAAGTAGA GAAGAATGCC 
AACAGATTAG CCACCAGCAA AGGACACAGG GGGCCAACTC TGCTTGTACA GGAATCTGTT 
GATTACCAGA GAAAAGATGC TGTTACTGAA GGCAATCATT CCCCAGTGTC ATCTCCAGGC 
AAAACAGCTC CTGTTAAAAA ACCCAGTGAT TTTGATCCTA GAAGAGAAAC CTCTGCATGC 
CACAATGCTG CTGGTCTCAA CAGCCCTAGG CGGTCACTCT GCTCAAGGGA TGTGCCTTTG 
ATTCAGATAG AGACAGATCA GAAAGAAGAG TGCATTGGAG AACCTGGACC TTTCCTTTCC 
CAAAGTGGCT CCCTAGAGGA AACAGAAGGC CACCAACCTG AAGAAACCAT CCCAGATGTG 
GCCAGAAATG AAGACACAGC TCCGAGCACC TGTCAGAGCT CTCGTGACAG CCTTGAGACC 
AGTGGGGAAG TAGAAGTGGA GGTCTTGAAA GAAGACATAC CCCGAGATGA GTCCCGGAAC 
CCTCCTAGCA GCAGCGAGGA GAGTACAGGC AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC 
ATCCCAGACG ACACAAGCAG CTTTCTGCAG CTCAGCGAGC GGTCCATGAG CAATGGCAAC 
AGTAGTGGCA CTAGCAGTCT TGGCATTATG GACCTGGACA TTTATCAGGA AAGCATACCG 
TCTTCTCCCA TGATTAATGA ATTAGTAGAA GAAAAGGAGA TTCTTAAAGA ACAATCAGAA 
AGCATAAAGG AACATGCCTC TGGACTGCCA GGGAGAGCTG CCAGCCCCCA GAGGAGCCTA 
CTGGTGATCA ACTTTGACCT GGAGCCAGAG TGTCCTGATG CTGAGCTTCG AGCCACTCTG 
CAGTGGATAG CTGCCTCCGA ACTGGGGATC CCAACAATCT ACTTTAAGAA ATCTCAGGAA 
AGCAGAATTG AAAAGTTTCT AGATGTTGTG AAGCTTGTTC AGCAGAAGTC CTGGAAGGTG 
GGAGATATAT TTCACGCGGT TGTCCAGTAC TGCAAACTGC ATGCAGAGCA GAAGGAGAGG 
ACTCCGAGTC TCTTTGACTG GCTTCTGGAA CTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( XM_006496576.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496639.1 Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X2, mRNA.

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CloneID	OMu27827
Clone ID Related Accession (Same CDS sequence)	XM_006496576.3 , XM_006496576.4
Accession Version	XM_006496576.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	A-kinase anchor protein SPHKAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496576.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTAG TTGAACCAAA CTCTGCTACC TGCCTTTCTG CTGCCCTGCC GGTCAAGAGA 
CACTGCCAAG AATCCAGCAA CGTGGAATCA CCACTGATGC ATGAAGGTTC AGAGCCACAG 
CAGATCACCA GCAGTGCAGC AGGCAACCTG GCTGGCTCCA TCACGGCCTG TAAAAAGGTT 
CTTCGAAGCA ATAGCTTGCT GGAGTCCACA GACTACTGGC TGCAGAACCA GAGGACGCCT 
TGCCAGATTG GGTTTGTGGA AGATGAGTCA GAAAACTGTG CTTCTGTCTG CTTTGTGAAC 
CTGGATGTCA ACAAAGATGC CTGCATCACA GAGAACCTGC AGCAGAAACT GGTCAATGTT 
TCACCGGACC TTCCAAACCT CATCAGCTCC ATGAATGTCC AACAGCCGAA AGAAAATGAA 
ATTGTCCTCC TAAGTGGGTT AGCCTCTGGA AACCTCCAGG CAGATTTTGA TGTTTCACAG 
TGCCCTTGGC TACCAGATAT CTGCTTGGTC CAGTGTGCAA GAGGGAACAG ACCAAACAGT 
ACCAACTGCA TCATCTTTGA GATCAACAAG TTCTTAATTG GTCTGGAAGT AGTGCAGGAG 
CGTCAGCTCC ACCTGGAGAC GAACGTGTTG AAGCTGGAGG ATGATACAAA CTGTTCTCTG 
TCTTCAATTG AAGAAGATTT TCTTACAGCC TCCGAGCATT TGGAGGAAGA AATCGAGGTG 
GACGACTGCA GAAGTGGTTT GGAAAATACA AATGTGTCAG CTAATGTTTT GGAAAGTAAA 
AAGCCAAAGG AAACTACCCA GGAAGGATGG GATTACCACA AGGAAAAGTT GCATTGTGCT 
TTAGGGGAAA AACACATTAG AAAACACCGT ACGCCCTCCA CAAAAACAGA AGGGTCAAAG 
GAAAATACAG AAGAGAACAC GTCCTTGAAA AGTCTCAATC GTTTAGTCAG GCCATCACAC 
CTGAAAAGTG AAGTGGCGGG GAACAAGCAA CTGGCCACAA ACTACTCCTA CCCAGAAAAT 
ATCAAAGGGG AACTAGAAAC ATCTCAAATG CTGTTCATCC CTAGAGATGC CTATCTCTCC 
ATGGTTAAGA AAGATGTACT TTCTCCGTGT AGTGTACTCA GTGAGCAGGG AGGCAGCCAC 
AGAGACCATG ACGTTACTCC AAACCCTCTT CCTCCCGTTC AAAATGGAGA AGCCAGCACC 
GGAGAGTATG CCACAAACTT AGCAGAATCT GTAATGCAAG ATGCCTTCAT CCGATTGTCT 
CAGTCACAGC CCACACTCCC CCAGGAATCT GCAGTCAGTT TCTCTATGCG AAGTGCTCTC 
CTTCCCAGTG GCTGTTGCAC AAAAGATATG GTGGTCCCTC GTTCATGGAA CGAGCTACCC 
AAAATTGTCA TTGTGCAGAG CCCAGATGGC AGTGACACAG TCCCTGAGCC AAATGTCTCC 
TCCTGGCCTG ACATGGAATT TGTTGAAACC TCGGGCATCT TTTCTGCAGA CAGTTCCAGC 
AGACCCACTC AGAGTGCCCT AGAAGTTGCA TTGGCTTGTG CAGCCACTGT GATTGGAACC 
ATTTCCAGTC CACAGGCCAC AGAAAGATTC GCCATGGAGC AAGAATCCCT GGTATCTACC 
TATGCCCAGA GAGGCACTGG GGTACAGCAA ACTCAAGTGC CCCAAGCATT CATGGCACCT 
TCTACAACCG AGTATTCCTT CCCATCAGCT CTGTGTGGCA TGACACAAGT GGCAAGTGCT 
GTCGCTGTCT GTGGCTTATG TGAAAAAGAA GAGGCGACAT GTCCTGTAGC TCCAACTGAC 
CTCTTGCCTA CATCTGGGGC TTCTGAAGAA ATATCCTCCA TCGGAAGTTT AGTGATGGAG 
AGGAGCACAG AACTGGGGAA GGAAGCCATT GCAGAGGCTC TGCTCAGAGA GGCTACTCTG 
ATTTTAGCAA GGCCAGATGC TTACAGCAGC CTTGGTGAGC TCTTGGAATC TGTGAACCAG 
AGAATCATAG AAACCACTTC TAAAACCCAG ACCCTGTGCA CAGAAAGTGT TCAAAGGAAT 
GAACTGGCAC ACACCTTGTC CAATGTCATC CTCAAGCACT CCGTGGATGA ACTTCACCAG 
AAAACTACAA TGGCTCACCC CACTGATGAA AGGCATCCCT GTGGAACTCT GGACACCTTG 
ATGGAAAGTG TGAACCAGCT GCTGCACAAT GTGATCTGCT TCACATTCAA AAAGATGAAT 
CACATTGTAA CACTCAGTGA GCATCCCTCA TTTGATCAGG CTGCTGGTCA AGCCTGGGTA 
AAAGCCTTTG CATGCCCCAG CAGCCAGCCT CTTAGCAATG CACACGGTAC TGGCCTTGTC 
ATCAGGAATC TTGTAGAGGA TGCATCTCCT AAGTCAAACA AGGGCGGAGC AAGGCCAGAA 
CTGGTCAACA ACCCCAGGTT GCAATCTGAA TTCTCCTGCA GTCATAGAAT GTTTGATTCA 
ACTGCTAAGT CATTCCCCAA GGAAATATAT CTGAAAGGGA TTATGGGAGA GGATACCAGG 
AACCCTCATC ATACACTAAA TTATGACAGC AATGAACGAA GAGCCTCTAC AGACCTAGGA 
AAATTGACAA CAGCAAGCGA GGGTTGTAGT GGTTTCCAGG AAACTGAAGA CAGCATTGTT 
CCAAACACCC AAGAGAAATA CATCTGTGCC ACACCCCTAA ACAATGAAGC TCAAGTTAAC 
CTATCCTTAT TAGGTGATGA CCTGTCTGTT CCTGCTCAGT CTACTCTAGA GGCAAAGCAG 
TCCGAGGTCT ATGGCATCAC AGATTTTGCG GAAGAATTGG CAGAGACTGT TGTCTCCATG 
GCAACTGAAA TTGCAGCAAT CTGCCTTGAC AACTCCAATG GCAAACAGCC CTGGTTTTGT 
GCTTGGAAAA GAGGGAACGA GTTTTTAACG GCGCCTAACG GATCCTGCCG ATCCTTGAAG 
AGGAAGAAGG AAAACTCAAG CGCCGGAAGC ACCGTGAGGA AACACAAGCC ACCTCGCCTC 
AGTGAAATCA AGAGAAAAGC TGATGAGCAC CCAGAGCTGA AAGAGAAGCT GATGAACAGA 
GTCATGGATG AGTCCATGAA CCTCGAAGAC ATCCCTGATT CCGTCAGTAC CTTTGCCAAT 
GAAGTGGCAG CCAAGATCAT GAACCTCACA GAGTTTTCCA TGGTGGATGG GGTGTGGCAA 
GGCCAGAGTT GTTCCCGGAC TCGGCTTCTG GGTGGCGATA GGTGGAACCG GCTGAAGGCC 
TCAAGCTGTG AGAGCATTCC TGAGGAGGAC TCTGAAGCCA GGGTCTTTGT AAACAGCCTG 
GGTTTGATGA GTACCTTAAG CCAACCAGTT AGCAGGGCCA GCTCCGTCTC CAAACAGTCC 
AGCTGTGAGA GCATCACCGA TGAGTTTTCC AGGTTCATGG TGAAACAGAT GGAAAACGAA 
GGGAGAGGGT TTGAGTTGCT GCTGGATTAC TACGCAGGCA AAAATGCCAG CAGTATCATG 
AGCTCAGCGA TGCAGCAGGC ATGCCAGAAA AACGACCACC TCAACGTGAG ACCGAGCTGC 
CCCTCTAAGC AATCCAGTAC AGAGAGCATA ACAGAGGAGT TCTATAGGTA CATGTTAAGG 
GACATCGCGA AAGAAAGCAA AGATGGTGCC TCCTCCAGAC GTAGCAGCCA TGATTGGACC 
ACGGGCCTGC TGTCTCCTTC TACACGATCC CCTCTGTGTT ACAGACAGTC ATCTATGCCT 
GACAGCAGGT CGCCATGCTC CAGACTAACA GTAAATGCGC CTGTCAAAGC CAACTCCTTA 
GATGGCTTTG CCCAAAACTG CCCTCAAGAT TCTGTAAATG TACAGCCAGT CAGTAGGGCT 
TCCTCATCTG GCCTCTGCAA ATCAGATTCC TGCTTGTATC GCAGAAGTGG GACTGACCAG 
ATCACAAACA TGCTAATTCA TGAAACATGG GCCAGCTCCA TTGAGGCTCT CATGAGAAAG 
AACAAAATCA TTGCTGATGA TAGCGAGGCA GCTAATGCCA GTCCTGGCCC TGTTTCCAGT 
GGTTCTCCTT TGCAAGTAGA GAAGAATGCC AACAGATTAG CCACCAGCAA AGGACACAGG 
GGGCCAACTC TGCTTGTACA GGAATCTGTT GATTACCAGA GAAAAGATGC TGTTACTGAA 
GGCAATCATT CCCCAGTGTC ATCTCCAGGC AAAACAGCTC CTGTTAAAAA ACCCAGTGAT 
TTTGATCCTA GAAGAGAAAC CTCTGCATGC CACAATGCTG CTGGTCTCAA CAGCCCTAGG 
CGGTCACTCT GCTCAAGGGA TGTGCCTTTG ATTCAGATAG AGACAGATCA GAAAGAAGAG 
TGCATTGGAG AACCTGGACC TTTCCTTTCC CAAAGTGGCT CCCTAGAGGA AACAGAAGGC 
CACCAACCTG AAGAAACCAT CCCAGATGTG GCCAGAAATG AAGACACAGC TCCGAGCACC 
TGTCAGAGCT CTCGTGACAG CCTTGAGACC AGTGGGGAAG TAGAAGTGGA GGTCTTGAAA 
GAAGACATAC CCCGAGATGA GTCCCGGAAC CCTCCTAGCA GCAGCGAGGA GAGTACAGGC 
AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC ATCCCAGACG ACACAAGCAG CTTTCTGCAG 
CTCAGCGAGC GGTCCATGAG CAATGGCAAC AGTAGTGGCA CTAGCAGTCT TGGCATTATG 
GACCTGGACA TTTATCAGGA AAGCATACCG TCTTCTCCCA TGATTAATGA ATTAGTAGAA 
GAAAAGGAGA TTCTTAAAGA ACAATCAGAA AGCATAAAGG AACATGCCTC TGGACTGCCA 
GGGAGAGCTG CCAGCCCCCA GAGGAGCCTA CTGGTGATCA ACTTTGACCT GGAGCCAGAG 
TGTCCTGATG CTGAGCTTCG AGCCACTCTG CAGTGGATAG CTGCCTCCGA ACTGGGGATC 
CCAACAATCT ACTTTAAGAA ATCTCAGGAA AGCAGAATTG AAAAGTTTCT AGATGTTGTG 
AAGCTTGTTC AGCAGAAGTC CTGGAAGGTG GGAGATATAT TTCACGCGGT TGTCCAGTAC 
TGCAAACTGC ATGCAGAGCA GAAGGAGAGG ACTCCGAGTC TCTTTGACTG GCTTCTGGAA 
CTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496639.1
CDS	320..5428
Translation	MWVVEPNSATCLSAALPVKRHCQESSNVESPLMHEGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASGNLQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETNVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETTQEGWDYHKEKLHCALGEKHIRKHRTPSTKTEGSKENTEENTSLKSLNRLVRPSHLKSEVAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKDVLSPCSVLSEQGGSHRDHDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSMRSALLPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADSSSRPTQSALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQAFMAPSTTEYSFPSALCGMTQVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSIGSLVMERSTELGKEAIAEALLREATLILARPDAYSSLGELLESVNQRIIETTSKTQTLCTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCGTLDTLMESVNQLLHNVICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLVEDASPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTLNYDSNERRASTDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLLGDDLSVPAQSTLEAKQSEVYGITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCSRTRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEFYRYMLRDIAKESKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVNAPVKANSLDGFAQNCPQDSVNVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQVEKNANRLATSKGHRGPTLLVQESVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAGLNSPRRSLCSRDVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSSRDSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLSERSMSNGNSSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESIKEHASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006496576.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496576.3

ORF Insert Sequence:

ATGTGGGTAG TTGAACCAAA CTCTGCTACC TGCCTTTCTG CTGCCCTGCC GGTCAAGAGA 
CACTGCCAAG AATCCAGCAA CGTGGAATCA CCACTGATGC ATGAAGGTTC AGAGCCACAG 
CAGATCACCA GCAGTGCAGC AGGCAACCTG GCTGGCTCCA TCACGGCCTG TAAAAAGGTT 
CTTCGAAGCA ATAGCTTGCT GGAGTCCACA GACTACTGGC TGCAGAACCA GAGGACGCCT 
TGCCAGATTG GGTTTGTGGA AGATGAGTCA GAAAACTGTG CTTCTGTCTG CTTTGTGAAC 
CTGGATGTCA ACAAAGATGC CTGCATCACA GAGAACCTGC AGCAGAAACT GGTCAATGTT 
TCACCGGACC TTCCAAACCT CATCAGCTCC ATGAATGTCC AACAGCCGAA AGAAAATGAA 
ATTGTCCTCC TAAGTGGGTT AGCCTCTGGA AACCTCCAGG CAGATTTTGA TGTTTCACAG 
TGCCCTTGGC TACCAGATAT CTGCTTGGTC CAGTGTGCAA GAGGGAACAG ACCAAACAGT 
ACCAACTGCA TCATCTTTGA GATCAACAAG TTCTTAATTG GTCTGGAAGT AGTGCAGGAG 
CGTCAGCTCC ACCTGGAGAC GAACGTGTTG AAGCTGGAGG ATGATACAAA CTGTTCTCTG 
TCTTCAATTG AAGAAGATTT TCTTACAGCC TCCGAGCATT TGGAGGAAGA AATCGAGGTG 
GACGACTGCA GAAGTGGTTT GGAAAATACA AATGTGTCAG CTAATGTTTT GGAAAGTAAA 
AAGCCAAAGG AAACTACCCA GGAAGGATGG GATTACCACA AGGAAAAGTT GCATTGTGCT 
TTAGGGGAAA AACACATTAG AAAACACCGT ACGCCCTCCA CAAAAACAGA AGGGTCAAAG 
GAAAATACAG AAGAGAACAC GTCCTTGAAA AGTCTCAATC GTTTAGTCAG GCCATCACAC 
CTGAAAAGTG AAGTGGCGGG GAACAAGCAA CTGGCCACAA ACTACTCCTA CCCAGAAAAT 
ATCAAAGGGG AACTAGAAAC ATCTCAAATG CTGTTCATCC CTAGAGATGC CTATCTCTCC 
ATGGTTAAGA AAGATGTACT TTCTCCGTGT AGTGTACTCA GTGAGCAGGG AGGCAGCCAC 
AGAGACCATG ACGTTACTCC AAACCCTCTT CCTCCCGTTC AAAATGGAGA AGCCAGCACC 
GGAGAGTATG CCACAAACTT AGCAGAATCT GTAATGCAAG ATGCCTTCAT CCGATTGTCT 
CAGTCACAGC CCACACTCCC CCAGGAATCT GCAGTCAGTT TCTCTATGCG AAGTGCTCTC 
CTTCCCAGTG GCTGTTGCAC AAAAGATATG GTGGTCCCTC GTTCATGGAA CGAGCTACCC 
AAAATTGTCA TTGTGCAGAG CCCAGATGGC AGTGACACAG TCCCTGAGCC AAATGTCTCC 
TCCTGGCCTG ACATGGAATT TGTTGAAACC TCGGGCATCT TTTCTGCAGA CAGTTCCAGC 
AGACCCACTC AGAGTGCCCT AGAAGTTGCA TTGGCTTGTG CAGCCACTGT GATTGGAACC 
ATTTCCAGTC CACAGGCCAC AGAAAGATTC GCCATGGAGC AAGAATCCCT GGTATCTACC 
TATGCCCAGA GAGGCACTGG GGTACAGCAA ACTCAAGTGC CCCAAGCATT CATGGCACCT 
TCTACAACCG AGTATTCCTT CCCATCAGCT CTGTGTGGCA TGACACAAGT GGCAAGTGCT 
GTCGCTGTCT GTGGCTTATG TGAAAAAGAA GAGGCGACAT GTCCTGTAGC TCCAACTGAC 
CTCTTGCCTA CATCTGGGGC TTCTGAAGAA ATATCCTCCA TCGGAAGTTT AGTGATGGAG 
AGGAGCACAG AACTGGGGAA GGAAGCCATT GCAGAGGCTC TGCTCAGAGA GGCTACTCTG 
ATTTTAGCAA GGCCAGATGC TTACAGCAGC CTTGGTGAGC TCTTGGAATC TGTGAACCAG 
AGAATCATAG AAACCACTTC TAAAACCCAG ACCCTGTGCA CAGAAAGTGT TCAAAGGAAT 
GAACTGGCAC ACACCTTGTC CAATGTCATC CTCAAGCACT CCGTGGATGA ACTTCACCAG 
AAAACTACAA TGGCTCACCC CACTGATGAA AGGCATCCCT GTGGAACTCT GGACACCTTG 
ATGGAAAGTG TGAACCAGCT GCTGCACAAT GTGATCTGCT TCACATTCAA AAAGATGAAT 
CACATTGTAA CACTCAGTGA GCATCCCTCA TTTGATCAGG CTGCTGGTCA AGCCTGGGTA 
AAAGCCTTTG CATGCCCCAG CAGCCAGCCT CTTAGCAATG CACACGGTAC TGGCCTTGTC 
ATCAGGAATC TTGTAGAGGA TGCATCTCCT AAGTCAAACA AGGGCGGAGC AAGGCCAGAA 
CTGGTCAACA ACCCCAGGTT GCAATCTGAA TTCTCCTGCA GTCATAGAAT GTTTGATTCA 
ACTGCTAAGT CATTCCCCAA GGAAATATAT CTGAAAGGGA TTATGGGAGA GGATACCAGG 
AACCCTCATC ATACACTAAA TTATGACAGC AATGAACGAA GAGCCTCTAC AGACCTAGGA 
AAATTGACAA CAGCAAGCGA GGGTTGTAGT GGTTTCCAGG AAACTGAAGA CAGCATTGTT 
CCAAACACCC AAGAGAAATA CATCTGTGCC ACACCCCTAA ACAATGAAGC TCAAGTTAAC 
CTATCCTTAT TAGGTGATGA CCTGTCTGTT CCTGCTCAGT CTACTCTAGA GGCAAAGCAG 
TCCGAGGTCT ATGGCATCAC AGATTTTGCG GAAGAATTGG CAGAGACTGT TGTCTCCATG 
GCAACTGAAA TTGCAGCAAT CTGCCTTGAC AACTCCAATG GCAAACAGCC CTGGTTTTGT 
GCTTGGAAAA GAGGGAACGA GTTTTTAACG GCGCCTAACG GATCCTGCCG ATCCTTGAAG 
AGGAAGAAGG AAAACTCAAG CGCCGGAAGC ACCGTGAGGA AACACAAGCC ACCTCGCCTC 
AGTGAAATCA AGAGAAAAGC TGATGAGCAC CCAGAGCTGA AAGAGAAGCT GATGAACAGA 
GTCATGGATG AGTCCATGAA CCTCGAAGAC ATCCCTGATT CCGTCAGTAC CTTTGCCAAT 
GAAGTGGCAG CCAAGATCAT GAACCTCACA GAGTTTTCCA TGGTGGATGG GGTGTGGCAA 
GGCCAGAGTT GTTCCCGGAC TCGGCTTCTG GGTGGCGATA GGTGGAACCG GCTGAAGGCC 
TCAAGCTGTG AGAGCATTCC TGAGGAGGAC TCTGAAGCCA GGGTCTTTGT AAACAGCCTG 
GGTTTGATGA GTACCTTAAG CCAACCAGTT AGCAGGGCCA GCTCCGTCTC CAAACAGTCC 
AGCTGTGAGA GCATCACCGA TGAGTTTTCC AGGTTCATGG TGAAACAGAT GGAAAACGAA 
GGGAGAGGGT TTGAGTTGCT GCTGGATTAC TACGCAGGCA AAAATGCCAG CAGTATCATG 
AGCTCAGCGA TGCAGCAGGC ATGCCAGAAA AACGACCACC TCAACGTGAG ACCGAGCTGC 
CCCTCTAAGC AATCCAGTAC AGAGAGCATA ACAGAGGAGT TCTATAGGTA CATGTTAAGG 
GACATCGCGA AAGAAAGCAA AGATGGTGCC TCCTCCAGAC GTAGCAGCCA TGATTGGACC 
ACGGGCCTGC TGTCTCCTTC TACACGATCC CCTCTGTGTT ACAGACAGTC ATCTATGCCT 
GACAGCAGGT CGCCATGCTC CAGACTAACA GTAAATGCGC CTGTCAAAGC CAACTCCTTA 
GATGGCTTTG CCCAAAACTG CCCTCAAGAT TCTGTAAATG TACAGCCAGT CAGTAGGGCT 
TCCTCATCTG GCCTCTGCAA ATCAGATTCC TGCTTGTATC GCAGAAGTGG GACTGACCAG 
ATCACAAACA TGCTAATTCA TGAAACATGG GCCAGCTCCA TTGAGGCTCT CATGAGAAAG 
AACAAAATCA TTGCTGATGA TAGCGAGGCA GCTAATGCCA GTCCTGGCCC TGTTTCCAGT 
GGTTCTCCTT TGCAAGTAGA GAAGAATGCC AACAGATTAG CCACCAGCAA AGGACACAGG 
GGGCCAACTC TGCTTGTACA GGAATCTGTT GATTACCAGA GAAAAGATGC TGTTACTGAA 
GGCAATCATT CCCCAGTGTC ATCTCCAGGC AAAACAGCTC CTGTTAAAAA ACCCAGTGAT 
TTTGATCCTA GAAGAGAAAC CTCTGCATGC CACAATGCTG CTGGTCTCAA CAGCCCTAGG 
CGGTCACTCT GCTCAAGGGA TGTGCCTTTG ATTCAGATAG AGACAGATCA GAAAGAAGAG 
TGCATTGGAG AACCTGGACC TTTCCTTTCC CAAAGTGGCT CCCTAGAGGA AACAGAAGGC 
CACCAACCTG AAGAAACCAT CCCAGATGTG GCCAGAAATG AAGACACAGC TCCGAGCACC 
TGTCAGAGCT CTCGTGACAG CCTTGAGACC AGTGGGGAAG TAGAAGTGGA GGTCTTGAAA 
GAAGACATAC CCCGAGATGA GTCCCGGAAC CCTCCTAGCA GCAGCGAGGA GAGTACAGGC 
AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC ATCCCAGACG ACACAAGCAG CTTTCTGCAG 
CTCAGCGAGC GGTCCATGAG CAATGGCAAC AGTAGTGGCA CTAGCAGTCT TGGCATTATG 
GACCTGGACA TTTATCAGGA AAGCATACCG TCTTCTCCCA TGATTAATGA ATTAGTAGAA 
GAAAAGGAGA TTCTTAAAGA ACAATCAGAA AGCATAAAGG AACATGCCTC TGGACTGCCA 
GGGAGAGCTG CCAGCCCCCA GAGGAGCCTA CTGGTGATCA ACTTTGACCT GGAGCCAGAG 
TGTCCTGATG CTGAGCTTCG AGCCACTCTG CAGTGGATAG CTGCCTCCGA ACTGGGGATC 
CCAACAATCT ACTTTAAGAA ATCTCAGGAA AGCAGAATTG AAAAGTTTCT AGATGTTGTG 
AAGCTTGTTC AGCAGAAGTC CTGGAAGGTG GGAGATATAT TTCACGCGGT TGTCCAGTAC 
TGCAAACTGC ATGCAGAGCA GAAGGAGAGG ACTCCGAGTC TCTTTGACTG GCTTCTGGAA 
CTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( XM_006496577.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496640.1 Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu27828
Clone ID Related Accession (Same CDS sequence)	XM_006496577.3 , XM_006496577.4
Accession Version	XM_006496577.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	A-kinase anchor protein SPHKAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496577.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTCA ACTCCCGGCT TTCTGTGCAA AGCAACGTGG AATCACCACT GATGCATGAA 
GGTTCAGAGC CACAGCAGAT CACCAGCAGT GCAGCAGGCA ACCTGGCTGG CTCCATCACG 
GCCTGTAAAA AGGTTCTTCG AAGCAATAGC TTGCTGGAGT CCACAGACTA CTGGCTGCAG 
AACCAGAGGA CGCCTTGCCA GATTGGGTTT GTGGAAGATG AGTCAGAAAA CTGTGCTTCT 
GTCTGCTTTG TGAACCTGGA TGTCAACAAA GATGCCTGCA TCACAGAGAA CCTGCAGCAG 
AAACTGGTCA ATGTTTCACC GGACCTTCCA AACCTCATCA GCTCCATGAA TGTCCAACAG 
CCGAAAGAAA ATGAAATTGT CCTCCTAAGT GGGTTAGCCT CTGGAAACCT CCAGGCAGAT 
TTTGATGTTT CACAGTGCCC TTGGCTACCA GATATCTGCT TGGTCCAGTG TGCAAGAGGG 
AACAGACCAA ACAGTACCAA CTGCATCATC TTTGAGATCA ACAAGTTCTT AATTGGTCTG 
GAAGTAGTGC AGGAGCGTCA GCTCCACCTG GAGACGAACG TGTTGAAGCT GGAGGATGAT 
ACAAACTGTT CTCTGTCTTC AATTGAAGAA GATTTTCTTA CAGCCTCCGA GCATTTGGAG 
GAAGAAATCG AGGTGGACGA CTGCAGAAGT GGTTTGGAAA ATACAAATGT GTCAGCTAAT 
GTTTTGGAAA GTAAAAAGCC AAAGGAAACT ACCCAGGAAG GATGGGATTA CCACAAGGAA 
AAGTTGCATT GTGCTTTAGG GGAAAAACAC ATTAGAAAAC ACCGTACGCC CTCCACAAAA 
ACAGAAGGGT CAAAGGAAAA TACAGAAGAG AACACGTCCT TGAAAAGTCT CAATCGTTTA 
GTCAGGCCAT CACACCTGAA AAGTGAAGTG GCGGGGAACA AGCAACTGGC CACAAACTAC 
TCCTACCCAG AAAATATCAA AGGGGAACTA GAAACATCTC AAATGCTGTT CATCCCTAGA 
GATGCCTATC TCTCCATGGT TAAGAAAGAT GTACTTTCTC CGTGTAGTGT ACTCAGTGAG 
CAGGGAGGCA GCCACAGAGA CCATGACGTT ACTCCAAACC CTCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA GCACCGGAGA GTATGCCACA AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC ACAGCCCACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
ATGCGAAGTG CTCTCCTTCC CAGTGGCTGT TGCACAAAAG ATATGGTGGT CCCTCGTTCA 
TGGAACGAGC TACCCAAAAT TGTCATTGTG CAGAGCCCAG ATGGCAGTGA CACAGTCCCT 
GAGCCAAATG TCTCCTCCTG GCCTGACATG GAATTTGTTG AAACCTCGGG CATCTTTTCT 
GCAGACAGTT CCAGCAGACC CACTCAGAGT GCCCTAGAAG TTGCATTGGC TTGTGCAGCC 
ACTGTGATTG GAACCATTTC CAGTCCACAG GCCACAGAAA GATTCGCCAT GGAGCAAGAA 
TCCCTGGTAT CTACCTATGC CCAGAGAGGC ACTGGGGTAC AGCAAACTCA AGTGCCCCAA 
GCATTCATGG CACCTTCTAC AACCGAGTAT TCCTTCCCAT CAGCTCTGTG TGGCATGACA 
CAAGTGGCAA GTGCTGTCGC TGTCTGTGGC TTATGTGAAA AAGAAGAGGC GACATGTCCT 
GTAGCTCCAA CTGACCTCTT GCCTACATCT GGGGCTTCTG AAGAAATATC CTCCATCGGA 
AGTTTAGTGA TGGAGAGGAG CACAGAACTG GGGAAGGAAG CCATTGCAGA GGCTCTGCTC 
AGAGAGGCTA CTCTGATTTT AGCAAGGCCA GATGCTTACA GCAGCCTTGG TGAGCTCTTG 
GAATCTGTGA ACCAGAGAAT CATAGAAACC ACTTCTAAAA CCCAGACCCT GTGCACAGAA 
AGTGTTCAAA GGAATGAACT GGCACACACC TTGTCCAATG TCATCCTCAA GCACTCCGTG 
GATGAACTTC ACCAGAAAAC TACAATGGCT CACCCCACTG ATGAAAGGCA TCCCTGTGGA 
ACTCTGGACA CCTTGATGGA AAGTGTGAAC CAGCTGCTGC ACAATGTGAT CTGCTTCACA 
TTCAAAAAGA TGAATCACAT TGTAACACTC AGTGAGCATC CCTCATTTGA TCAGGCTGCT 
GGTCAAGCCT GGGTAAAAGC CTTTGCATGC CCCAGCAGCC AGCCTCTTAG CAATGCACAC 
GGTACTGGCC TTGTCATCAG GAATCTTGTA GAGGATGCAT CTCCTAAGTC AAACAAGGGC 
GGAGCAAGGC CAGAACTGGT CAACAACCCC AGGTTGCAAT CTGAATTCTC CTGCAGTCAT 
AGAATGTTTG ATTCAACTGC TAAGTCATTC CCCAAGGAAA TATATCTGAA AGGGATTATG 
GGAGAGGATA CCAGGAACCC TCATCATACA CTAAATTATG ACAGCAATGA ACGAAGAGCC 
TCTACAGACC TAGGAAAATT GACAACAGCA AGCGAGGGTT GTAGTGGTTT CCAGGAAACT 
GAAGACAGCA TTGTTCCAAA CACCCAAGAG AAATACATCT GTGCCACACC CCTAAACAAT 
GAAGCTCAAG TTAACCTATC CTTATTAGGT GATGACCTGT CTGTTCCTGC TCAGTCTACT 
CTAGAGGCAA AGCAGTCCGA GGTCTATGGC ATCACAGATT TTGCGGAAGA ATTGGCAGAG 
ACTGTTGTCT CCATGGCAAC TGAAATTGCA GCAATCTGCC TTGACAACTC CAATGGCAAA 
CAGCCCTGGT TTTGTGCTTG GAAAAGAGGG AACGAGTTTT TAACGGCGCC TAACGGATCC 
TGCCGATCCT TGAAGAGGAA GAAGGAAAAC TCAAGCGCCG GAAGCACCGT GAGGAAACAC 
AAGCCACCTC GCCTCAGTGA AATCAAGAGA AAAGCTGATG AGCACCCAGA GCTGAAAGAG 
AAGCTGATGA ACAGAGTCAT GGATGAGTCC ATGAACCTCG AAGACATCCC TGATTCCGTC 
AGTACCTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TCACAGAGTT TTCCATGGTG 
GATGGGGTGT GGCAAGGCCA GAGTTGTTCC CGGACTCGGC TTCTGGGTGG CGATAGGTGG 
AACCGGCTGA AGGCCTCAAG CTGTGAGAGC ATTCCTGAGG AGGACTCTGA AGCCAGGGTC 
TTTGTAAACA GCCTGGGTTT GATGAGTACC TTAAGCCAAC CAGTTAGCAG GGCCAGCTCC 
GTCTCCAAAC AGTCCAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAA 
CAGATGGAAA ACGAAGGGAG AGGGTTTGAG TTGCTGCTGG ATTACTACGC AGGCAAAAAT 
GCCAGCAGTA TCATGAGCTC AGCGATGCAG CAGGCATGCC AGAAAAACGA CCACCTCAAC 
GTGAGACCGA GCTGCCCCTC TAAGCAATCC AGTACAGAGA GCATAACAGA GGAGTTCTAT 
AGGTACATGT TAAGGGACAT CGCGAAAGAA AGCAAAGATG GTGCCTCCTC CAGACGTAGC 
AGCCATGATT GGACCACGGG CCTGCTGTCT CCTTCTACAC GATCCCCTCT GTGTTACAGA 
CAGTCATCTA TGCCTGACAG CAGGTCGCCA TGCTCCAGAC TAACAGTAAA TGCGCCTGTC 
AAAGCCAACT CCTTAGATGG CTTTGCCCAA AACTGCCCTC AAGATTCTGT AAATGTACAG 
CCAGTCAGTA GGGCTTCCTC ATCTGGCCTC TGCAAATCAG ATTCCTGCTT GTATCGCAGA 
AGTGGGACTG ACCAGATCAC AAACATGCTA ATTCATGAAA CATGGGCCAG CTCCATTGAG 
GCTCTCATGA GAAAGAACAA AATCATTGCT GATGATAGCG AGGCAGCTAA TGCCAGTCCT 
GGCCCTGTTT CCAGTGGTTC TCCTTTGCAA GTAGAGAAGA ATGCCAACAG ATTAGCCACC 
AGCAAAGGAC ACAGGGGGCC AACTCTGCTT GTACAGGAAT CTGTTGATTA CCAGAGAAAA 
GATGCTGTTA CTGAAGGCAA TCATTCCCCA GTGTCATCTC CAGGCAAAAC AGCTCCTGTT 
AAAAAACCCA GTGATTTTGA TCCTAGAAGA GAAACCTCTG CATGCCACAA TGCTGCTGGT 
CTCAACAGCC CTAGGCGGTC ACTCTGCTCA AGGGATGTGC CTTTGATTCA GATAGAGACA 
GATCAGAAAG AAGAGTGCAT TGGAGAACCT GGACCTTTCC TTTCCCAAAG TGGCTCCCTA 
GAGGAAACAG AAGGCCACCA ACCTGAAGAA ACCATCCCAG ATGTGGCCAG AAATGAAGAC 
ACAGCTCCGA GCACCTGTCA GAGCTCTCGT GACAGCCTTG AGACCAGTGG GGAAGTAGAA 
GTGGAGGTCT TGAAAGAAGA CATACCCCGA GATGAGTCCC GGAACCCTCC TAGCAGCAGC 
GAGGAGAGTA CAGGCAGCTG GTCCCAGCTG GCCAATGAGG AGGACATCCC AGACGACACA 
AGCAGCTTTC TGCAGCTCAG CGAGCGGTCC ATGAGCAATG GCAACAGTAG TGGCACTAGC 
AGTCTTGGCA TTATGGACCT GGACATTTAT CAGGAAAGCA TACCGTCTTC TCCCATGATT 
AATGAATTAG TAGAAGAAAA GGAGATTCTT AAAGAACAAT CAGAAAGCAT AAAGGAACAT 
GCCTCTGGAC TGCCAGGGAG AGCTGCCAGC CCCCAGAGGA GCCTACTGGT GATCAACTTT 
GACCTGGAGC CAGAGTGTCC TGATGCTGAG CTTCGAGCCA CTCTGCAGTG GATAGCTGCC 
TCCGAACTGG GGATCCCAAC AATCTACTTT AAGAAATCTC AGGAAAGCAG AATTGAAAAG 
TTTCTAGATG TTGTGAAGCT TGTTCAGCAG AAGTCCTGGA AGGTGGGAGA TATATTTCAC 
GCGGTTGTCC AGTACTGCAA ACTGCATGCA GAGCAGAAGG AGAGGACTCC GAGTCTCTTT 
GACTGGCTTC TGGAACTAGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496640.1
CDS	383..5446
Translation	MDVNSRLSVQSNVESPLMHEGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASGNLQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETNVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETTQEGWDYHKEKLHCALGEKHIRKHRTPSTKTEGSKENTEENTSLKSLNRLVRPSHLKSEVAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKDVLSPCSVLSEQGGSHRDHDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSMRSALLPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADSSSRPTQSALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQAFMAPSTTEYSFPSALCGMTQVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSIGSLVMERSTELGKEAIAEALLREATLILARPDAYSSLGELLESVNQRIIETTSKTQTLCTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCGTLDTLMESVNQLLHNVICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLVEDASPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTLNYDSNERRASTDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLLGDDLSVPAQSTLEAKQSEVYGITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCSRTRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEFYRYMLRDIAKESKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVNAPVKANSLDGFAQNCPQDSVNVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQVEKNANRLATSKGHRGPTLLVQESVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAGLNSPRRSLCSRDVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSSRDSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLSERSMSNGNSSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESIKEHASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006496577.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496577.3

ORF Insert Sequence:

ATGGATGTCA ACTCCCGGCT TTCTGTGCAA AGCAACGTGG AATCACCACT GATGCATGAA 
GGTTCAGAGC CACAGCAGAT CACCAGCAGT GCAGCAGGCA ACCTGGCTGG CTCCATCACG 
GCCTGTAAAA AGGTTCTTCG AAGCAATAGC TTGCTGGAGT CCACAGACTA CTGGCTGCAG 
AACCAGAGGA CGCCTTGCCA GATTGGGTTT GTGGAAGATG AGTCAGAAAA CTGTGCTTCT 
GTCTGCTTTG TGAACCTGGA TGTCAACAAA GATGCCTGCA TCACAGAGAA CCTGCAGCAG 
AAACTGGTCA ATGTTTCACC GGACCTTCCA AACCTCATCA GCTCCATGAA TGTCCAACAG 
CCGAAAGAAA ATGAAATTGT CCTCCTAAGT GGGTTAGCCT CTGGAAACCT CCAGGCAGAT 
TTTGATGTTT CACAGTGCCC TTGGCTACCA GATATCTGCT TGGTCCAGTG TGCAAGAGGG 
AACAGACCAA ACAGTACCAA CTGCATCATC TTTGAGATCA ACAAGTTCTT AATTGGTCTG 
GAAGTAGTGC AGGAGCGTCA GCTCCACCTG GAGACGAACG TGTTGAAGCT GGAGGATGAT 
ACAAACTGTT CTCTGTCTTC AATTGAAGAA GATTTTCTTA CAGCCTCCGA GCATTTGGAG 
GAAGAAATCG AGGTGGACGA CTGCAGAAGT GGTTTGGAAA ATACAAATGT GTCAGCTAAT 
GTTTTGGAAA GTAAAAAGCC AAAGGAAACT ACCCAGGAAG GATGGGATTA CCACAAGGAA 
AAGTTGCATT GTGCTTTAGG GGAAAAACAC ATTAGAAAAC ACCGTACGCC CTCCACAAAA 
ACAGAAGGGT CAAAGGAAAA TACAGAAGAG AACACGTCCT TGAAAAGTCT CAATCGTTTA 
GTCAGGCCAT CACACCTGAA AAGTGAAGTG GCGGGGAACA AGCAACTGGC CACAAACTAC 
TCCTACCCAG AAAATATCAA AGGGGAACTA GAAACATCTC AAATGCTGTT CATCCCTAGA 
GATGCCTATC TCTCCATGGT TAAGAAAGAT GTACTTTCTC CGTGTAGTGT ACTCAGTGAG 
CAGGGAGGCA GCCACAGAGA CCATGACGTT ACTCCAAACC CTCTTCCTCC CGTTCAAAAT 
GGAGAAGCCA GCACCGGAGA GTATGCCACA AACTTAGCAG AATCTGTAAT GCAAGATGCC 
TTCATCCGAT TGTCTCAGTC ACAGCCCACA CTCCCCCAGG AATCTGCAGT CAGTTTCTCT 
ATGCGAAGTG CTCTCCTTCC CAGTGGCTGT TGCACAAAAG ATATGGTGGT CCCTCGTTCA 
TGGAACGAGC TACCCAAAAT TGTCATTGTG CAGAGCCCAG ATGGCAGTGA CACAGTCCCT 
GAGCCAAATG TCTCCTCCTG GCCTGACATG GAATTTGTTG AAACCTCGGG CATCTTTTCT 
GCAGACAGTT CCAGCAGACC CACTCAGAGT GCCCTAGAAG TTGCATTGGC TTGTGCAGCC 
ACTGTGATTG GAACCATTTC CAGTCCACAG GCCACAGAAA GATTCGCCAT GGAGCAAGAA 
TCCCTGGTAT CTACCTATGC CCAGAGAGGC ACTGGGGTAC AGCAAACTCA AGTGCCCCAA 
GCATTCATGG CACCTTCTAC AACCGAGTAT TCCTTCCCAT CAGCTCTGTG TGGCATGACA 
CAAGTGGCAA GTGCTGTCGC TGTCTGTGGC TTATGTGAAA AAGAAGAGGC GACATGTCCT 
GTAGCTCCAA CTGACCTCTT GCCTACATCT GGGGCTTCTG AAGAAATATC CTCCATCGGA 
AGTTTAGTGA TGGAGAGGAG CACAGAACTG GGGAAGGAAG CCATTGCAGA GGCTCTGCTC 
AGAGAGGCTA CTCTGATTTT AGCAAGGCCA GATGCTTACA GCAGCCTTGG TGAGCTCTTG 
GAATCTGTGA ACCAGAGAAT CATAGAAACC ACTTCTAAAA CCCAGACCCT GTGCACAGAA 
AGTGTTCAAA GGAATGAACT GGCACACACC TTGTCCAATG TCATCCTCAA GCACTCCGTG 
GATGAACTTC ACCAGAAAAC TACAATGGCT CACCCCACTG ATGAAAGGCA TCCCTGTGGA 
ACTCTGGACA CCTTGATGGA AAGTGTGAAC CAGCTGCTGC ACAATGTGAT CTGCTTCACA 
TTCAAAAAGA TGAATCACAT TGTAACACTC AGTGAGCATC CCTCATTTGA TCAGGCTGCT 
GGTCAAGCCT GGGTAAAAGC CTTTGCATGC CCCAGCAGCC AGCCTCTTAG CAATGCACAC 
GGTACTGGCC TTGTCATCAG GAATCTTGTA GAGGATGCAT CTCCTAAGTC AAACAAGGGC 
GGAGCAAGGC CAGAACTGGT CAACAACCCC AGGTTGCAAT CTGAATTCTC CTGCAGTCAT 
AGAATGTTTG ATTCAACTGC TAAGTCATTC CCCAAGGAAA TATATCTGAA AGGGATTATG 
GGAGAGGATA CCAGGAACCC TCATCATACA CTAAATTATG ACAGCAATGA ACGAAGAGCC 
TCTACAGACC TAGGAAAATT GACAACAGCA AGCGAGGGTT GTAGTGGTTT CCAGGAAACT 
GAAGACAGCA TTGTTCCAAA CACCCAAGAG AAATACATCT GTGCCACACC CCTAAACAAT 
GAAGCTCAAG TTAACCTATC CTTATTAGGT GATGACCTGT CTGTTCCTGC TCAGTCTACT 
CTAGAGGCAA AGCAGTCCGA GGTCTATGGC ATCACAGATT TTGCGGAAGA ATTGGCAGAG 
ACTGTTGTCT CCATGGCAAC TGAAATTGCA GCAATCTGCC TTGACAACTC CAATGGCAAA 
CAGCCCTGGT TTTGTGCTTG GAAAAGAGGG AACGAGTTTT TAACGGCGCC TAACGGATCC 
TGCCGATCCT TGAAGAGGAA GAAGGAAAAC TCAAGCGCCG GAAGCACCGT GAGGAAACAC 
AAGCCACCTC GCCTCAGTGA AATCAAGAGA AAAGCTGATG AGCACCCAGA GCTGAAAGAG 
AAGCTGATGA ACAGAGTCAT GGATGAGTCC ATGAACCTCG AAGACATCCC TGATTCCGTC 
AGTACCTTTG CCAATGAAGT GGCAGCCAAG ATCATGAACC TCACAGAGTT TTCCATGGTG 
GATGGGGTGT GGCAAGGCCA GAGTTGTTCC CGGACTCGGC TTCTGGGTGG CGATAGGTGG 
AACCGGCTGA AGGCCTCAAG CTGTGAGAGC ATTCCTGAGG AGGACTCTGA AGCCAGGGTC 
TTTGTAAACA GCCTGGGTTT GATGAGTACC TTAAGCCAAC CAGTTAGCAG GGCCAGCTCC 
GTCTCCAAAC AGTCCAGCTG TGAGAGCATC ACCGATGAGT TTTCCAGGTT CATGGTGAAA 
CAGATGGAAA ACGAAGGGAG AGGGTTTGAG TTGCTGCTGG ATTACTACGC AGGCAAAAAT 
GCCAGCAGTA TCATGAGCTC AGCGATGCAG CAGGCATGCC AGAAAAACGA CCACCTCAAC 
GTGAGACCGA GCTGCCCCTC TAAGCAATCC AGTACAGAGA GCATAACAGA GGAGTTCTAT 
AGGTACATGT TAAGGGACAT CGCGAAAGAA AGCAAAGATG GTGCCTCCTC CAGACGTAGC 
AGCCATGATT GGACCACGGG CCTGCTGTCT CCTTCTACAC GATCCCCTCT GTGTTACAGA 
CAGTCATCTA TGCCTGACAG CAGGTCGCCA TGCTCCAGAC TAACAGTAAA TGCGCCTGTC 
AAAGCCAACT CCTTAGATGG CTTTGCCCAA AACTGCCCTC AAGATTCTGT AAATGTACAG 
CCAGTCAGTA GGGCTTCCTC ATCTGGCCTC TGCAAATCAG ATTCCTGCTT GTATCGCAGA 
AGTGGGACTG ACCAGATCAC AAACATGCTA ATTCATGAAA CATGGGCCAG CTCCATTGAG 
GCTCTCATGA GAAAGAACAA AATCATTGCT GATGATAGCG AGGCAGCTAA TGCCAGTCCT 
GGCCCTGTTT CCAGTGGTTC TCCTTTGCAA GTAGAGAAGA ATGCCAACAG ATTAGCCACC 
AGCAAAGGAC ACAGGGGGCC AACTCTGCTT GTACAGGAAT CTGTTGATTA CCAGAGAAAA 
GATGCTGTTA CTGAAGGCAA TCATTCCCCA GTGTCATCTC CAGGCAAAAC AGCTCCTGTT 
AAAAAACCCA GTGATTTTGA TCCTAGAAGA GAAACCTCTG CATGCCACAA TGCTGCTGGT 
CTCAACAGCC CTAGGCGGTC ACTCTGCTCA AGGGATGTGC CTTTGATTCA GATAGAGACA 
GATCAGAAAG AAGAGTGCAT TGGAGAACCT GGACCTTTCC TTTCCCAAAG TGGCTCCCTA 
GAGGAAACAG AAGGCCACCA ACCTGAAGAA ACCATCCCAG ATGTGGCCAG AAATGAAGAC 
ACAGCTCCGA GCACCTGTCA GAGCTCTCGT GACAGCCTTG AGACCAGTGG GGAAGTAGAA 
GTGGAGGTCT TGAAAGAAGA CATACCCCGA GATGAGTCCC GGAACCCTCC TAGCAGCAGC 
GAGGAGAGTA CAGGCAGCTG GTCCCAGCTG GCCAATGAGG AGGACATCCC AGACGACACA 
AGCAGCTTTC TGCAGCTCAG CGAGCGGTCC ATGAGCAATG GCAACAGTAG TGGCACTAGC 
AGTCTTGGCA TTATGGACCT GGACATTTAT CAGGAAAGCA TACCGTCTTC TCCCATGATT 
AATGAATTAG TAGAAGAAAA GGAGATTCTT AAAGAACAAT CAGAAAGCAT AAAGGAACAT 
GCCTCTGGAC TGCCAGGGAG AGCTGCCAGC CCCCAGAGGA GCCTACTGGT GATCAACTTT 
GACCTGGAGC CAGAGTGTCC TGATGCTGAG CTTCGAGCCA CTCTGCAGTG GATAGCTGCC 
TCCGAACTGG GGATCCCAAC AATCTACTTT AAGAAATCTC AGGAAAGCAG AATTGAAAAG 
TTTCTAGATG TTGTGAAGCT TGTTCAGCAG AAGTCCTGGA AGGTGGGAGA TATATTTCAC 
GCGGTTGTCC AGTACTGCAA ACTGCATGCA GAGCAGAAGG AGAGGACTCC GAGTCTCTTT 
GACTGGCTTC TGGAACTAGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphkap ( XM_006496578.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496641.1 Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X4, mRNA.

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CloneID	OMu27829
Clone ID Related Accession (Same CDS sequence)	XM_006496578.3 , NM_001356547.1
Accession Version	XM_006496578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	A-kinase anchor protein SPHKAP isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496578.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCTGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC ATGAAGGTTC AGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAACCTG 
GCTGGCTCCA TCACGGCCTG TAAAAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGC TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGATGAGTCA 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAAGATGC CTGCATCACA 
GAGAACCTGC AGCAGAAACT GGTCAATGTT TCACCGGACC TTCCAAACCT CATCAGCTCC 
ATGAATGTCC AACAGCCGAA AGAAAATGAA ATTGTCCTCC TAAGTGGGTT AGCCTCTGGA 
AACCTCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGTGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT AGTGCAGGAG CGTCAGCTCC ACCTGGAGAC GAACGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCTCTG TCTTCAATTG AAGAAGATTT TCTTACAGCC 
TCCGAGCATT TGGAGGAAGA AATCGAGGTG GACGACTGCA GAAGTGGTTT GGAAAATACA 
AATGTGTCAG CTAATGTTTT GGAAAGTAAA AAGCCAAAGG AAACTACCCA GGAAGGATGG 
GATTACCACA AGGAAAAGTT GCATTGTGCT TTAGGGGAAA AACACATTAG AAAACACCGT 
ACGCCCTCCA CAAAAACAGA AGGGTCAAAG GAAAATACAG AAGAGAACAC GTCCTTGAAA 
AGTCTCAATC GTTTAGTCAG GCCATCACAC CTGAAAAGTG AAGTGGCGGG GAACAAGCAA 
CTGGCCACAA ACTACTCCTA CCCAGAAAAT ATCAAAGGGG AACTAGAAAC ATCTCAAATG 
CTGTTCATCC CTAGAGATGC CTATCTCTCC ATGGTTAAGA AAGATGTACT TTCTCCGTGT 
AGTGTACTCA GTGAGCAGGG AGGCAGCCAC AGAGACCATG ACGTTACTCC AAACCCTCTT 
CCTCCCGTTC AAAATGGAGA AGCCAGCACC GGAGAGTATG CCACAAACTT AGCAGAATCT 
GTAATGCAAG ATGCCTTCAT CCGATTGTCT CAGTCACAGC CCACACTCCC CCAGGAATCT 
GCAGTCAGTT TCTCTATGCG AAGTGCTCTC CTTCCCAGTG GCTGTTGCAC AAAAGATATG 
GTGGTCCCTC GTTCATGGAA CGAGCTACCC AAAATTGTCA TTGTGCAGAG CCCAGATGGC 
AGTGACACAG TCCCTGAGCC AAATGTCTCC TCCTGGCCTG ACATGGAATT TGTTGAAACC 
TCGGGCATCT TTTCTGCAGA CAGTTCCAGC AGACCCACTC AGAGTGCCCT AGAAGTTGCA 
TTGGCTTGTG CAGCCACTGT GATTGGAACC ATTTCCAGTC CACAGGCCAC AGAAAGATTC 
GCCATGGAGC AAGAATCCCT GGTATCTACC TATGCCCAGA GAGGCACTGG GGTACAGCAA 
ACTCAAGTGC CCCAAGCATT CATGGCACCT TCTACAACCG AGTATTCCTT CCCATCAGCT 
CTGTGTGGCA TGACACAAGT GGCAAGTGCT GTCGCTGTCT GTGGCTTATG TGAAAAAGAA 
GAGGCGACAT GTCCTGTAGC TCCAACTGAC CTCTTGCCTA CATCTGGGGC TTCTGAAGAA 
ATATCCTCCA TCGGAAGTTT AGTGATGGAG AGGAGCACAG AACTGGGGAA GGAAGCCATT 
GCAGAGGCTC TGCTCAGAGA GGCTACTCTG ATTTTAGCAA GGCCAGATGC TTACAGCAGC 
CTTGGTGAGC TCTTGGAATC TGTGAACCAG AGAATCATAG AAACCACTTC TAAAACCCAG 
ACCCTGTGCA CAGAAAGTGT TCAAAGGAAT GAACTGGCAC ACACCTTGTC CAATGTCATC 
CTCAAGCACT CCGTGGATGA ACTTCACCAG AAAACTACAA TGGCTCACCC CACTGATGAA 
AGGCATCCCT GTGGAACTCT GGACACCTTG ATGGAAAGTG TGAACCAGCT GCTGCACAAT 
GTGATCTGCT TCACATTCAA AAAGATGAAT CACATTGTAA CACTCAGTGA GCATCCCTCA 
TTTGATCAGG CTGCTGGTCA AGCCTGGGTA AAAGCCTTTG CATGCCCCAG CAGCCAGCCT 
CTTAGCAATG CACACGGTAC TGGCCTTGTC ATCAGGAATC TTGTAGAGGA TGCATCTCCT 
AAGTCAAACA AGGGCGGAGC AAGGCCAGAA CTGGTCAACA ACCCCAGGTT GCAATCTGAA 
TTCTCCTGCA GTCATAGAAT GTTTGATTCA ACTGCTAAGT CATTCCCCAA GGAAATATAT 
CTGAAAGGGA TTATGGGAGA GGATACCAGG AACCCTCATC ATACACTAAA TTATGACAGC 
AATGAACGAA GAGCCTCTAC AGACCTAGGA AAATTGACAA CAGCAAGCGA GGGTTGTAGT 
GGTTTCCAGG AAACTGAAGA CAGCATTGTT CCAAACACCC AAGAGAAATA CATCTGTGCC 
ACACCCCTAA ACAATGAAGC TCAAGTTAAC CTATCCTTAT TAGGTGATGA CCTGTCTGTT 
CCTGCTCAGT CTACTCTAGA GGCAAAGCAG TCCGAGGTCT ATGGCATCAC AGATTTTGCG 
GAAGAATTGG CAGAGACTGT TGTCTCCATG GCAACTGAAA TTGCAGCAAT CTGCCTTGAC 
AACTCCAATG GCAAACAGCC CTGGTTTTGT GCTTGGAAAA GAGGGAACGA GTTTTTAACG 
GCGCCTAACG GATCCTGCCG ATCCTTGAAG AGGAAGAAGG AAAACTCAAG CGCCGGAAGC 
ACCGTGAGGA AACACAAGCC ACCTCGCCTC AGTGAAATCA AGAGAAAAGC TGATGAGCAC 
CCAGAGCTGA AAGAGAAGCT GATGAACAGA GTCATGGATG AGTCCATGAA CCTCGAAGAC 
ATCCCTGATT CCGTCAGTAC CTTTGCCAAT GAAGTGGCAG CCAAGATCAT GAACCTCACA 
GAGTTTTCCA TGGTGGATGG GGTGTGGCAA GGCCAGAGTT GTTCCCGGAC TCGGCTTCTG 
GGTGGCGATA GGTGGAACCG GCTGAAGGCC TCAAGCTGTG AGAGCATTCC TGAGGAGGAC 
TCTGAAGCCA GGGTCTTTGT AAACAGCCTG GGTTTGATGA GTACCTTAAG CCAACCAGTT 
AGCAGGGCCA GCTCCGTCTC CAAACAGTCC AGCTGTGAGA GCATCACCGA TGAGTTTTCC 
AGGTTCATGG TGAAACAGAT GGAAAACGAA GGGAGAGGGT TTGAGTTGCT GCTGGATTAC 
TACGCAGGCA AAAATGCCAG CAGTATCATG AGCTCAGCGA TGCAGCAGGC ATGCCAGAAA 
AACGACCACC TCAACGTGAG ACCGAGCTGC CCCTCTAAGC AATCCAGTAC AGAGAGCATA 
ACAGAGGAGT TCTATAGGTA CATGTTAAGG GACATCGCGA AAGAAAGCAA AGATGGTGCC 
TCCTCCAGAC GTAGCAGCCA TGATTGGACC ACGGGCCTGC TGTCTCCTTC TACACGATCC 
CCTCTGTGTT ACAGACAGTC ATCTATGCCT GACAGCAGGT CGCCATGCTC CAGACTAACA 
GTAAATGCGC CTGTCAAAGC CAACTCCTTA GATGGCTTTG CCCAAAACTG CCCTCAAGAT 
TCTGTAAATG TACAGCCAGT CAGTAGGGCT TCCTCATCTG GCCTCTGCAA ATCAGATTCC 
TGCTTGTATC GCAGAAGTGG GACTGACCAG ATCACAAACA TGCTAATTCA TGAAACATGG 
GCCAGCTCCA TTGAGGCTCT CATGAGAAAG AACAAAATCA TTGCTGATGA TAGCGAGGCA 
GCTAATGCCA GTCCTGGCCC TGTTTCCAGT GGTTCTCCTT TGCAAGTAGA GAAGAATGCC 
AACAGATTAG CCACCAGCAA AGGACACAGG GGGCCAACTC TGCTTGTACA GGAATCTGTT 
GATTACCAGA GAAAAGATGC TGTTACTGAA GGCAATCATT CCCCAGTGTC ATCTCCAGGC 
AAAACAGCTC CTGTTAAAAA ACCCAGTGAT TTTGATCCTA GAAGAGAAAC CTCTGCATGC 
CACAATGCTG CTGGTCTCAA CAGCCCTAGG CGGTCACTCT GCTCAAGGGA TGTGCCTTTG 
ATTCAGATAG AGACAGATCA GAAAGAAGAG TGCATTGGAG AACCTGGACC TTTCCTTTCC 
CAAAGTGGCT CCCTAGAGGA AACAGAAGGC CACCAACCTG AAGAAACCAT CCCAGATGTG 
GCCAGAAATG AAGACACAGC TCCGAGCACC TGTCAGAGCT CTCGTGACAG CCTTGAGACC 
AGTGGGGAAG TAGAAGTGGA GGTCTTGAAA GAAGACATAC CCCGAGATGA GTCCCGGAAC 
CCTCCTAGCA GCAGCGAGGA GAGTACAGGC AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC 
ATCCCAGACG ACACAAGCAG CTTTCTGCAG CTCAGCGAGC GGTCCATGAG TGAATTAGTA 
GAAGAAAAGG AGATTCTTAA AGAACAATCA GAAAGCATAA AGGAACATGC CTCTGGACTG 
CCAGGGAGAG CTGCCAGCCC CCAGAGGAGC CTACTGGTGA TCAACTTTGA CCTGGAGCCA 
GAGTGTCCTG ATGCTGAGCT TCGAGCCACT CTGCAGTGGA TAGCTGCCTC CGAACTGGGG 
ATCCCAACAA TCTACTTTAA GAAATCTCAG GAAAGCAGAA TTGAAAAGTT TCTAGATGTT 
GTGAAGCTTG TTCAGCAGAA GTCCTGGAAG GTGGGAGATA TATTTCACGC GGTTGTCCAG 
TACTGCAAAC TGCATGCAGA GCAGAAGGAG AGGACTCCGA GTCTCTTTGA CTGGCTTCTG 
GAACTAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496641.1
CDS	183..5234
Translation	MSTPGFLCKAMWVVEPNSATCLSAALPVKRHCQESSNVESPLMHEGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASGNLQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETNVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETTQEGWDYHKEKLHCALGEKHIRKHRTPSTKTEGSKENTEENTSLKSLNRLVRPSHLKSEVAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKDVLSPCSVLSEQGGSHRDHDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSMRSALLPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADSSSRPTQSALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQAFMAPSTTEYSFPSALCGMTQVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSIGSLVMERSTELGKEAIAEALLREATLILARPDAYSSLGELLESVNQRIIETTSKTQTLCTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCGTLDTLMESVNQLLHNVICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLVEDASPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTLNYDSNERRASTDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLLGDDLSVPAQSTLEAKQSEVYGITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAWKRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCSRTRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEFYRYMLRDIAKESKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVNAPVKANSLDGFAQNCPQDSVNVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQVEKNANRLATSKGHRGPTLLVQESVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAGLNSPRRSLCSRDVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSSRDSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLSERSMSELVEEKEILKEQSESIKEHASGLPGRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG

Target ORF information:

RefSeq Version	XM_006496578.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496578.3

ORF Insert Sequence:

ATGTCAACTC CCGGCTTTCT GTGCAAAGCC ATGTGGGTAG TTGAACCAAA CTCTGCTACC 
TGCCTTTCTG CTGCCCTGCC GGTCAAGAGA CACTGCCAAG AATCCAGCAA CGTGGAATCA 
CCACTGATGC ATGAAGGTTC AGAGCCACAG CAGATCACCA GCAGTGCAGC AGGCAACCTG 
GCTGGCTCCA TCACGGCCTG TAAAAAGGTT CTTCGAAGCA ATAGCTTGCT GGAGTCCACA 
GACTACTGGC TGCAGAACCA GAGGACGCCT TGCCAGATTG GGTTTGTGGA AGATGAGTCA 
GAAAACTGTG CTTCTGTCTG CTTTGTGAAC CTGGATGTCA ACAAAGATGC CTGCATCACA 
GAGAACCTGC AGCAGAAACT GGTCAATGTT TCACCGGACC TTCCAAACCT CATCAGCTCC 
ATGAATGTCC AACAGCCGAA AGAAAATGAA ATTGTCCTCC TAAGTGGGTT AGCCTCTGGA 
AACCTCCAGG CAGATTTTGA TGTTTCACAG TGCCCTTGGC TACCAGATAT CTGCTTGGTC 
CAGTGTGCAA GAGGGAACAG ACCAAACAGT ACCAACTGCA TCATCTTTGA GATCAACAAG 
TTCTTAATTG GTCTGGAAGT AGTGCAGGAG CGTCAGCTCC ACCTGGAGAC GAACGTGTTG 
AAGCTGGAGG ATGATACAAA CTGTTCTCTG TCTTCAATTG AAGAAGATTT TCTTACAGCC 
TCCGAGCATT TGGAGGAAGA AATCGAGGTG GACGACTGCA GAAGTGGTTT GGAAAATACA 
AATGTGTCAG CTAATGTTTT GGAAAGTAAA AAGCCAAAGG AAACTACCCA GGAAGGATGG 
GATTACCACA AGGAAAAGTT GCATTGTGCT TTAGGGGAAA AACACATTAG AAAACACCGT 
ACGCCCTCCA CAAAAACAGA AGGGTCAAAG GAAAATACAG AAGAGAACAC GTCCTTGAAA 
AGTCTCAATC GTTTAGTCAG GCCATCACAC CTGAAAAGTG AAGTGGCGGG GAACAAGCAA 
CTGGCCACAA ACTACTCCTA CCCAGAAAAT ATCAAAGGGG AACTAGAAAC ATCTCAAATG 
CTGTTCATCC CTAGAGATGC CTATCTCTCC ATGGTTAAGA AAGATGTACT TTCTCCGTGT 
AGTGTACTCA GTGAGCAGGG AGGCAGCCAC AGAGACCATG ACGTTACTCC AAACCCTCTT 
CCTCCCGTTC AAAATGGAGA AGCCAGCACC GGAGAGTATG CCACAAACTT AGCAGAATCT 
GTAATGCAAG ATGCCTTCAT CCGATTGTCT CAGTCACAGC CCACACTCCC CCAGGAATCT 
GCAGTCAGTT TCTCTATGCG AAGTGCTCTC CTTCCCAGTG GCTGTTGCAC AAAAGATATG 
GTGGTCCCTC GTTCATGGAA CGAGCTACCC AAAATTGTCA TTGTGCAGAG CCCAGATGGC 
AGTGACACAG TCCCTGAGCC AAATGTCTCC TCCTGGCCTG ACATGGAATT TGTTGAAACC 
TCGGGCATCT TTTCTGCAGA CAGTTCCAGC AGACCCACTC AGAGTGCCCT AGAAGTTGCA 
TTGGCTTGTG CAGCCACTGT GATTGGAACC ATTTCCAGTC CACAGGCCAC AGAAAGATTC 
GCCATGGAGC AAGAATCCCT GGTATCTACC TATGCCCAGA GAGGCACTGG GGTACAGCAA 
ACTCAAGTGC CCCAAGCATT CATGGCACCT TCTACAACCG AGTATTCCTT CCCATCAGCT 
CTGTGTGGCA TGACACAAGT GGCAAGTGCT GTCGCTGTCT GTGGCTTATG TGAAAAAGAA 
GAGGCGACAT GTCCTGTAGC TCCAACTGAC CTCTTGCCTA CATCTGGGGC TTCTGAAGAA 
ATATCCTCCA TCGGAAGTTT AGTGATGGAG AGGAGCACAG AACTGGGGAA GGAAGCCATT 
GCAGAGGCTC TGCTCAGAGA GGCTACTCTG ATTTTAGCAA GGCCAGATGC TTACAGCAGC 
CTTGGTGAGC TCTTGGAATC TGTGAACCAG AGAATCATAG AAACCACTTC TAAAACCCAG 
ACCCTGTGCA CAGAAAGTGT TCAAAGGAAT GAACTGGCAC ACACCTTGTC CAATGTCATC 
CTCAAGCACT CCGTGGATGA ACTTCACCAG AAAACTACAA TGGCTCACCC CACTGATGAA 
AGGCATCCCT GTGGAACTCT GGACACCTTG ATGGAAAGTG TGAACCAGCT GCTGCACAAT 
GTGATCTGCT TCACATTCAA AAAGATGAAT CACATTGTAA CACTCAGTGA GCATCCCTCA 
TTTGATCAGG CTGCTGGTCA AGCCTGGGTA AAAGCCTTTG CATGCCCCAG CAGCCAGCCT 
CTTAGCAATG CACACGGTAC TGGCCTTGTC ATCAGGAATC TTGTAGAGGA TGCATCTCCT 
AAGTCAAACA AGGGCGGAGC AAGGCCAGAA CTGGTCAACA ACCCCAGGTT GCAATCTGAA 
TTCTCCTGCA GTCATAGAAT GTTTGATTCA ACTGCTAAGT CATTCCCCAA GGAAATATAT 
CTGAAAGGGA TTATGGGAGA GGATACCAGG AACCCTCATC ATACACTAAA TTATGACAGC 
AATGAACGAA GAGCCTCTAC AGACCTAGGA AAATTGACAA CAGCAAGCGA GGGTTGTAGT 
GGTTTCCAGG AAACTGAAGA CAGCATTGTT CCAAACACCC AAGAGAAATA CATCTGTGCC 
ACACCCCTAA ACAATGAAGC TCAAGTTAAC CTATCCTTAT TAGGTGATGA CCTGTCTGTT 
CCTGCTCAGT CTACTCTAGA GGCAAAGCAG TCCGAGGTCT ATGGCATCAC AGATTTTGCG 
GAAGAATTGG CAGAGACTGT TGTCTCCATG GCAACTGAAA TTGCAGCAAT CTGCCTTGAC 
AACTCCAATG GCAAACAGCC CTGGTTTTGT GCTTGGAAAA GAGGGAACGA GTTTTTAACG 
GCGCCTAACG GATCCTGCCG ATCCTTGAAG AGGAAGAAGG AAAACTCAAG CGCCGGAAGC 
ACCGTGAGGA AACACAAGCC ACCTCGCCTC AGTGAAATCA AGAGAAAAGC TGATGAGCAC 
CCAGAGCTGA AAGAGAAGCT GATGAACAGA GTCATGGATG AGTCCATGAA CCTCGAAGAC 
ATCCCTGATT CCGTCAGTAC CTTTGCCAAT GAAGTGGCAG CCAAGATCAT GAACCTCACA 
GAGTTTTCCA TGGTGGATGG GGTGTGGCAA GGCCAGAGTT GTTCCCGGAC TCGGCTTCTG 
GGTGGCGATA GGTGGAACCG GCTGAAGGCC TCAAGCTGTG AGAGCATTCC TGAGGAGGAC 
TCTGAAGCCA GGGTCTTTGT AAACAGCCTG GGTTTGATGA GTACCTTAAG CCAACCAGTT 
AGCAGGGCCA GCTCCGTCTC CAAACAGTCC AGCTGTGAGA GCATCACCGA TGAGTTTTCC 
AGGTTCATGG TGAAACAGAT GGAAAACGAA GGGAGAGGGT TTGAGTTGCT GCTGGATTAC 
TACGCAGGCA AAAATGCCAG CAGTATCATG AGCTCAGCGA TGCAGCAGGC ATGCCAGAAA 
AACGACCACC TCAACGTGAG ACCGAGCTGC CCCTCTAAGC AATCCAGTAC AGAGAGCATA 
ACAGAGGAGT TCTATAGGTA CATGTTAAGG GACATCGCGA AAGAAAGCAA AGATGGTGCC 
TCCTCCAGAC GTAGCAGCCA TGATTGGACC ACGGGCCTGC TGTCTCCTTC TACACGATCC 
CCTCTGTGTT ACAGACAGTC ATCTATGCCT GACAGCAGGT CGCCATGCTC CAGACTAACA 
GTAAATGCGC CTGTCAAAGC CAACTCCTTA GATGGCTTTG CCCAAAACTG CCCTCAAGAT 
TCTGTAAATG TACAGCCAGT CAGTAGGGCT TCCTCATCTG GCCTCTGCAA ATCAGATTCC 
TGCTTGTATC GCAGAAGTGG GACTGACCAG ATCACAAACA TGCTAATTCA TGAAACATGG 
GCCAGCTCCA TTGAGGCTCT CATGAGAAAG AACAAAATCA TTGCTGATGA TAGCGAGGCA 
GCTAATGCCA GTCCTGGCCC TGTTTCCAGT GGTTCTCCTT TGCAAGTAGA GAAGAATGCC 
AACAGATTAG CCACCAGCAA AGGACACAGG GGGCCAACTC TGCTTGTACA GGAATCTGTT 
GATTACCAGA GAAAAGATGC TGTTACTGAA GGCAATCATT CCCCAGTGTC ATCTCCAGGC 
AAAACAGCTC CTGTTAAAAA ACCCAGTGAT TTTGATCCTA GAAGAGAAAC CTCTGCATGC 
CACAATGCTG CTGGTCTCAA CAGCCCTAGG CGGTCACTCT GCTCAAGGGA TGTGCCTTTG 
ATTCAGATAG AGACAGATCA GAAAGAAGAG TGCATTGGAG AACCTGGACC TTTCCTTTCC 
CAAAGTGGCT CCCTAGAGGA AACAGAAGGC CACCAACCTG AAGAAACCAT CCCAGATGTG 
GCCAGAAATG AAGACACAGC TCCGAGCACC TGTCAGAGCT CTCGTGACAG CCTTGAGACC 
AGTGGGGAAG TAGAAGTGGA GGTCTTGAAA GAAGACATAC CCCGAGATGA GTCCCGGAAC 
CCTCCTAGCA GCAGCGAGGA GAGTACAGGC AGCTGGTCCC AGCTGGCCAA TGAGGAGGAC 
ATCCCAGACG ACACAAGCAG CTTTCTGCAG CTCAGCGAGC GGTCCATGAG TGAATTAGTA 
GAAGAAAAGG AGATTCTTAA AGAACAATCA GAAAGCATAA AGGAACATGC CTCTGGACTG 
CCAGGGAGAG CTGCCAGCCC CCAGAGGAGC CTACTGGTGA TCAACTTTGA CCTGGAGCCA 
GAGTGTCCTG ATGCTGAGCT TCGAGCCACT CTGCAGTGGA TAGCTGCCTC CGAACTGGGG 
ATCCCAACAA TCTACTTTAA GAAATCTCAG GAAAGCAGAA TTGAAAAGTT TCTAGATGTT 
GTGAAGCTTG TTCAGCAGAA GTCCTGGAAG GTGGGAGATA TATTTCACGC GGTTGTCCAG 
TACTGCAAAC TGCATGCAGA GCAGAAGGAG AGGACTCCGA GTCTCTTTGA CTGGCTTCTG 
GAACTAGGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Sphkap
Entrez Gene ID	77629
Full Name	SPHK1 interactor, AKAP domain containing
Synonyms	4930544G21Rik,A930009L15Rik,AI852220,SKIP,mKIAA1678
General protein information	Preferred Names SPHK1 interactor, AKAP domain containing Names A-kinase anchor protein SPHKAP SPHK1-interactor and AKAP domain-containing protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C5

Canis lupus familiaris (dog)	SPHKAP	XP_005635852.1
Gallus gallus (chicken)	SPHKAP	NP_001171003.1
Xenopus tropicalis (tropical clawed frog)	sphkap	XP_002933413.2
Bos taurus (cattle)	SPHKAP	XP_002685689.2
Mus musculus (house mouse)	Sphkap	NP_766018.3
Danio rerio (zebrafish)	sphkap	NP_001076514.1
Homo sapiens (human)	SPHKAP	NP_001136116.1
Pan troglodytes (chimpanzee)	SPHKAP	XP_001137841.1
	SPHKAP	XP_001111587.1
Rattus norvegicus (Norway rat)	Sphkap	NP_001120964.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Sphkap cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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