tlk-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	tlk-1
Entrez Gene ID	176369
Full Name	Serine/threonine-protein kinase tousled-like 1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_181913.3	NP_871642.2	Serine/threonine-protein kinase tousled-like 1
NM_066744.4	NP_499145.2	Serine/threonine-protein kinase tousled-like 1
NM_066744.3	NP_499145.2	Serine/threonine-protein kinase tousled-like 1
NM_001047379.1	NP_001040844.1	Serine/threonine-protein kinase tousled-like 1
NM_001268088.2	NP_001255017.1	Protein kinase domain-containing protein
NM_001268088.1	NP_001255017.1	Serine/threonine-protein kinase tousled-like 1
NM_181913.4	NP_871642.2	Serine/threonine-protein kinase tousled-like 1
NM_001047379.2	NP_001040844.1	Protein kinase domain-containing protein

Pathways from BioSystems

Homologies

	TLK2	XP_001107302.2
Canis lupus familiaris (dog)	TLK2	XP_005624288.1
Danio rerio (zebrafish)	tlk2	NP_001103887.1
Caenorhabditis elegans (roundworm)	tlk-1	NP_001040844.1
Xenopus tropicalis (tropical clawed frog)	tlk2	NP_001072711.1
Homo sapiens (human)	TLK2	NP_006843.2
Gallus gallus (chicken)	TLK2	XP_418070.3
Drosophila melanogaster (fruit fly)	tlk	NP_001138153.1
Mus musculus (house mouse)	Tlk2	NP_001106176.1
Pan troglodytes (chimpanzee)	TLK2	XP_523598.3
Rattus norvegicus (Norway rat)	Tlk2	NP_001178581.1
Bos taurus (cattle)	TLK2	NP_001179756.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TLK-1 contributes to chromosome condensation and segregation;TLK-1 functions in cytokinesis by localizing AIR-2 to the midzone microtubules.
Title: Caenorhabditis elegans TLK-1 controls cytokinesis by localizing AIR-2/Aurora B to midzone microtubules.

Tousled-like kinase (TLK-1) contributes to chromosome segregation as a substrate and regulator of the Aurora B kinase AIR-2.
Title: The C. elegans Tousled-like kinase contributes to chromosome segregation as a substrate and regulator of the Aurora B kinase.

The following tlk-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tlk-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340104	NM_066744.4 Latest version!	Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa340105	NM_181913.4 Latest version!	Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa340531	NM_001268088.2 Latest version!	Caenorhabditis elegans Protein kinase domain-containing protein (tlk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa340577	NM_001047379.2 Latest version!	Caenorhabditis elegans Protein kinase domain-containing protein (tlk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa340104	NM_066744.3	Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCa340531	NM_001268088.1	Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCa340105	NM_181913.3	Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCa340577	NM_001047379.1	Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tlk-1 ( NM_066744.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499145.2 Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), mRNA.

Price & Availability↑

CloneID	OCa340104
Clone ID Related Accession (Same CDS sequence)	NM_066744.4 , NM_066744.3
Accession Version	NM_066744.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase tousled-like 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_066744.3.

ATGTCGATGT TGTCAATGGA CGGTATCGTC GCAGGAGGCG GAAGCAGCAG TGGTGGCGGA 
GAACGAAGTT TCGTATTAGA GCAAAAGATG TTCAATACGG GCCCACAGAA CAAAGCTTTA 
CCAACGGTCC AGAGCAGCGG TTCATCATCG AATCACGCTC CGATCGTCGG CGAGTCTCCA 
CTCGGCACTG TGAGCAGCAC AATGGCTACC GGTGATACGG GTCGTGTTGG AAACGTGACC 
TATATGAGTT CCGGAATGCT CGGTGCAACA CAGTTTATGC CACAAAATTC AAGTCATCCA 
TCAACGTCTG TAATGATGCA ACAGGTACCA CCACAAAACG GTGGTGCAAC ACGTTCATCA 
CCAACTGAGA TGCAACAGTG TATGCAGGCA ATGTCAGAAG ACTCGATAGA AATGCGGGAT 
TATAATTCTG GAGTTCATCA TATGCATCCT CATCAAATGC AAATGCAACA GCAGCAGCAA 
CATCATCAGC AGCAATACAA TATGTCGTAT CATAATCATC AACAACAAAT GCAGCAGATG 
CATTACCACC AGCAGCAACA GCAATATCAG CAGCAGCAGG CTCAACATCA TCAGATGTAT 
GCTCCACAAA TTCAGCAACA GCAACAGCAG CCCCAGCAAC AGTCACAACA ACAGTCTGCC 
CAACAGCCCC AACAGTCATC GGCTGCGTTA CAACATGTGA ATGAGTCGAG CAATCTCTCG 
TCGGCTGGTA GTATTTCGGA TCGTGAACCA GAGCAACACG GTGGTACCCC TCAACGACCG 
ACAGCGCCAC AGTCGTCGAC AGCCACTGAC AAGAAAACCA GAAAGCGACG AAAAGCTGGT 
CCAACTGAAG ATCAGGCAAC TCCGAAACAA GAAAGGAAAA TCACAGAATT CATGAAAGTT 
GGAGGAGAGG TTGCAAGTGG AAATAGTGTT GCACGATGTC TATTGACGGA ATATCATCAA 
AATCAAGGAT CACCGAAGAG GCAGCCAGCT GTACAGCAGA ATGGATCTAA CAGTTACGAT 
TCACAGCAGC AGCAACCACA AATGAATCAG CATGAAATGC AAAACTCATA TTGGGGAGTT 
GCTACACCGT CATTGGGTGT TAATAATCGT GGAACCCCGA CTCCAACTCA ACAACAACAT 
TATTCATCGG ATTCAAACTC AAACTCGAAT CAATCACCAC CTGGTCAGGG AAATCAATCA 
GGAAGGATGG TGAGAACAAT CGATGAAGAG ACTCAGACGG ATTCTTCGCT ATCTCAGGCT 
AATCCTCAAA ATGCTGATGA AGTCGCCAAA ATGAATAGAA TCATTGAAGA CCATCGTCGC 
CAAATCGAAG AGTTGAACTC GAAGAATAGT TTGGAACGAA GAAAGAATGA AGCTTCGAAA 
GAAACAATCA AGCGATTACT TATCGATAAG AATCAGATCG AACGAAAAGC CCTTCGCGAC 
AAAACGGCTG CCGATTCACC TCGAATTGGA TGTTTCAAAA CTACTCGAAC TGGTGATTCA 
TTCCGTGATC AATGGGTTGA TGGGTGGGCG TTTGCCGAAA TGGATAAGAA AACTGAACAG 
ATCAATGCTG AAAGAAATGA AATTGCAAGT GCTTCTGCCT TGTTGAAAAA ACGAAAGCCT 
CTTGGAATCG GTAAAGAACC TAAACGCCCA CAGGCAGTCA ATTCTCAAAA TGACTCCAAC 
GGTATGCAGC CATCAACAAG TTCCAATACG AATGGAGACG ATGCGATCTT CAGACGACCT 
GAAGAACCGA AAGAAATCCA GTACCAAGAG TACATTGAGC TCGATGAAAT CTATAAACTG 
CGCAGGGAGC ACTTGAGAAA AGAAGAAACT GATCTCTCCA TGGAGAAAGA AAGATTGGAA 
AAGGAGAAGC AGCATCATGT GAGAGAGCTG AAACGAGCTT CGAATGAAAG TGCGTCACAG 
TTCAATGATC ATCGACTCTT GCATAAAAGA TACCTCATGC TGAACTTGCT CGGTAAGGGT 
GGATTCAGTG AGGTCTGGAA GGCGTTCGAC ATTGAAGAGA ACCGGTATGT CGCATGCAAG 
ATCCATCATG TTAACAAAGA CTGGAAGGAG GAGAAGAAGG CGAACTACGT CAAGCACGCG 
ATGCGTGAAA AAGACATCCA CAAGTCATTG GATCATTGCC GAATTGTGAA GCAATACGAT 
CTGCTCACAA TCGACAATCA CTCGTTCTGC ACGGTTCTCG AATATGTGCC GGGCAACGAC 
CTCGATTTCT ACCTCAAGCA GAACAGGTCG ATCAGTGAGA AGGAGGCTCG AAGCATTATT 
ATGCAAGTTG TTTCTGCTCT TGTCTATCTG AATGAAAAGA GCACTCCCAT CATTCATTAT 
GATCTAAAGC CTGCGAATAT TCTTCTCGAG TCAGGAAACA CCAGCGGTGC AATCAAGATC 
ACCGACTTTG GGCTGTCGAA AATCATGGAG GGAGAGAGTG ACGATCATGA CTTGGGAATC 
GAGCTCACCT CACAGTTCGC TGGAACTTAT TGGTATCTTC CACCTGAGAC CTTCATCGTT 
CCACCACCAA AGATCACTTG TAAAGTCGAT GTCTGGAGTA TTGGAGTCAT CTTCTATCAA 
TGTATCTATG GAAAGAAGCC GTTCGGAAAT GATCTGACTC AACAAAAGAT CCTCGAATAC 
AATACGATTA TTAATGCAAG AGAAGTGTCA TTCCCAAGCA AGCCTCAGGT GAGCTCCGCT 
GCTCAAGATT TCATAAGAAG ATGCCTGCAG TACAGGAAAG AAGACAGAGC AGATGTTTTC 
GAGCTGGCGA AGCATGAGCT TTTCAGACCA AGAGGAGCGA TAAGAGCCTC CGTTGCCGGA 
TCCGTAAGTT CTCCAAGCAT TCCACGCAGT CCATCAGTGA ATCGTGAAGA TGACAATATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499145.2
CDS	123..3005
Translation	MSMLSMDGIVAGGGSSSGGGERSFVLEQKMFNTGPQNKALPTVQSSGSSSNHAPIVGESPLGTVSSTMATGDTGRVGNVTYMSSGMLGATQFMPQNSSHPSTSVMMQQVPPQNGGATRSSPTEMQQCMQAMSEDSIEMRDYNSGVHHMHPHQMQMQQQQQHHQQQYNMSYHNHQQQMQQMHYHQQQQQYQQQQAQHHQMYAPQIQQQQQQPQQQSQQQSAQQPQQSSAALQHVNESSNLSSAGSISDREPEQHGGTPQRPTAPQSSTATDKKTRKRRKAGPTEDQATPKQERKITEFMKVGGEVASGNSVARCLLTEYHQNQGSPKRQPAVQQNGSNSYDSQQQQPQMNQHEMQNSYWGVATPSLGVNNRGTPTPTQQQHYSSDSNSNSNQSPPGQGNQSGRMVRTIDEETQTDSSLSQANPQNADEVAKMNRIIEDHRRQIEELNSKNSLERRKNEASKETIKRLLIDKNQIERKALRDKTAADSPRIGCFKTTRTGDSFRDQWVDGWAFAEMDKKTEQINAERNEIASASALLKKRKPLGIGKEPKRPQAVNSQNDSNGMQPSTSSNTNGDDAIFRRPEEPKEIQYQEYIELDEIYKLRREHLRKEETDLSMEKERLEKEKQHHVRELKRASNESASQFNDHRLLHKRYLMLNLLGKGGFSEVWKAFDIEENRYVACKIHHVNKDWKEEKKANYVKHAMREKDIHKSLDHCRIVKQYDLLTIDNHSFCTVLEYVPGNDLDFYLKQNRSISEKEARSIIMQVVSALVYLNEKSTPIIHYDLKPANILLESGNTSGAIKITDFGLSKIMEGESDDHDLGIELTSQFAGTYWYLPPETFIVPPPKITCKVDVWSIGVIFYQCIYGKKPFGNDLTQQKILEYNTIINAREVSFPSKPQVSSAAQDFIRRCLQYRKEDRADVFELAKHELFRPRGAIRASVAGSVSSPSIPRSPSVNREDDNM

Target ORF information:

RefSeq Version	NM_066744.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066744.4

ORF Insert Sequence:

ATGTCGATGT TGTCAATGGA CGGTATCGTC GCAGGAGGCG GAAGCAGCAG TGGTGGCGGA 
GAACGAAGTT TCGTATTAGA GCAAAAGATG TTCAATACGG GCCCACAGAA CAAAGCTTTA 
CCAACGGTCC AGAGCAGCGG TTCATCATCG AATCACGCTC CGATCGTCGG CGAGTCTCCA 
CTCGGCACTG TGAGCAGCAC AATGGCTACC GGTGATACGG GTCGTGTTGG AAACGTGACC 
TATATGAGTT CCGGAATGCT CGGTGCAACA CAGTTTATGC CACAAAATTC AAGTCATCCA 
TCAACGTCTG TAATGATGCA ACAGGTACCA CCACAAAACG GTGGTGCAAC ACGTTCATCA 
CCAACTGAGA TGCAACAGTG TATGCAGGCA ATGTCAGAAG ACTCGATAGA AATGCGGGAT 
TATAATTCTG GAGTTCATCA TATGCATCCT CATCAAATGC AAATGCAACA GCAGCAGCAA 
CATCATCAGC AGCAATACAA TATGTCGTAT CATAATCATC AACAACAAAT GCAGCAGATG 
CATTACCACC AGCAGCAACA GCAATATCAG CAGCAGCAGG CTCAACATCA TCAGATGTAT 
GCTCCACAAA TTCAGCAACA GCAACAGCAG CCCCAGCAAC AGTCACAACA ACAGTCTGCC 
CAACAGCCCC AACAGTCATC GGCTGCGTTA CAACATGTGA ATGAGTCGAG CAATCTCTCG 
TCGGCTGGTA GTATTTCGGA TCGTGAACCA GAGCAACACG GTGGTACCCC TCAACGACCG 
ACAGCGCCAC AGTCGTCGAC AGCCACTGAC AAGAAAACCA GAAAGCGACG AAAAGCTGGT 
CCAACTGAAG ATCAGGCAAC TCCGAAACAA GAAAGGAAAA TCACAGAATT CATGAAAGTT 
GGAGGAGAGG TTGCAAGTGG AAATAGTGTT GCACGATGTC TATTGACGGA ATATCATCAA 
AATCAAGGAT CACCGAAGAG GCAGCCAGCT GTACAGCAGA ATGGATCTAA CAGTTACGAT 
TCACAGCAGC AGCAACCACA AATGAATCAG CATGAAATGC AAAACTCATA TTGGGGAGTT 
GCTACACCGT CATTGGGTGT TAATAATCGT GGAACCCCGA CTCCAACTCA ACAACAACAT 
TATTCATCGG ATTCAAACTC AAACTCGAAT CAATCACCAC CTGGTCAGGG AAATCAATCA 
GGAAGGATGG TGAGAACAAT CGATGAAGAG ACTCAGACGG ATTCTTCGCT ATCTCAGGCT 
AATCCTCAAA ATGCTGATGA AGTCGCCAAA ATGAATAGAA TCATTGAAGA CCATCGTCGC 
CAAATCGAAG AGTTGAACTC GAAGAATAGT TTGGAACGAA GAAAGAATGA AGCTTCGAAA 
GAAACAATCA AGCGATTACT TATCGATAAG AATCAGATCG AACGAAAAGC CCTTCGCGAC 
AAAACGGCTG CCGATTCACC TCGAATTGGA TGTTTCAAAA CTACTCGAAC TGGTGATTCA 
TTCCGTGATC AATGGGTTGA TGGGTGGGCG TTTGCCGAAA TGGATAAGAA AACTGAACAG 
ATCAATGCTG AAAGAAATGA AATTGCAAGT GCTTCTGCCT TGTTGAAAAA ACGAAAGCCT 
CTTGGAATCG GTAAAGAACC TAAACGCCCA CAGGCAGTCA ATTCTCAAAA TGACTCCAAC 
GGTATGCAGC CATCAACAAG TTCCAATACG AATGGAGACG ATGCGATCTT CAGACGACCT 
GAAGAACCGA AAGAAATCCA GTACCAAGAG TACATTGAGC TCGATGAAAT CTATAAACTG 
CGCAGGGAGC ACTTGAGAAA AGAAGAAACT GATCTCTCCA TGGAGAAAGA AAGATTGGAA 
AAGGAGAAGC AGCATCATGT GAGAGAGCTG AAACGAGCTT CGAATGAAAG TGCGTCACAG 
TTCAATGATC ATCGACTCTT GCATAAAAGA TACCTCATGC TGAACTTGCT CGGTAAGGGT 
GGATTCAGTG AGGTCTGGAA GGCGTTCGAC ATTGAAGAGA ACCGGTATGT CGCATGCAAG 
ATCCATCATG TTAACAAAGA CTGGAAGGAG GAGAAGAAGG CGAACTACGT CAAGCACGCG 
ATGCGTGAAA AAGACATCCA CAAGTCATTG GATCATTGCC GAATTGTGAA GCAATACGAT 
CTGCTCACAA TCGACAATCA CTCGTTCTGC ACGGTTCTCG AATATGTGCC GGGCAACGAC 
CTCGATTTCT ACCTCAAGCA GAACAGGTCG ATCAGTGAGA AGGAGGCTCG AAGCATTATT 
ATGCAAGTTG TTTCTGCTCT TGTCTATCTG AATGAAAAGA GCACTCCCAT CATTCATTAT 
GATCTAAAGC CTGCGAATAT TCTTCTCGAG TCAGGAAACA CCAGCGGTGC AATCAAGATC 
ACCGACTTTG GGCTGTCGAA AATCATGGAG GGAGAGAGTG ACGATCATGA CTTGGGAATC 
GAGCTCACCT CACAGTTCGC TGGAACTTAT TGGTATCTTC CACCTGAGAC CTTCATCGTT 
CCACCACCAA AGATCACTTG TAAAGTCGAT GTCTGGAGTA TTGGAGTCAT CTTCTATCAA 
TGTATCTATG GAAAGAAGCC GTTCGGAAAT GATCTGACTC AACAAAAGAT CCTCGAATAC 
AATACGATTA TTAATGCAAG AGAAGTGTCA TTCCCAAGCA AGCCTCAGGT GAGCTCCGCT 
GCTCAAGATT TCATAAGAAG ATGCCTGCAG TACAGGAAAG AAGACAGAGC AGATGTTTTC 
GAGCTGGCGA AGCATGAGCT TTTCAGACCA AGAGGAGCGA TAAGAGCCTC CGTTGCCGGA 
TCCGTAAGTT CTCCAAGCAT TCCACGCAGT CCATCAGTGA ATCGTGAAGA TGACAATATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tlk-1 ( NM_181913.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_871642.2 Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), mRNA.

Price & Availability↑

CloneID	OCa340105
Clone ID Related Accession (Same CDS sequence)	NM_181913.3 , NM_181913.4
Accession Version	NM_181913.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase tousled-like 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_181913.3.

ATGTCGATGT TGTCAATGGA CGGTATCGTC GCAGGAGGCG GAAGCAGCAG TGGTGGCGGA 
GAACGAAGTT TCGTATTAGA GCAAAAGATG TTCAATACGG GCCCACAGAA CAAAGCTTTA 
CCAACGGTCC AGAGCAGCGG TTCATCATCG AATCACGCTC CGATCGTCGG CGAGTCTCCA 
CTCGGCACTG TGAGCAGCAC AATGGCTACC GGTGATACGG GTCGTGTTGG AAACGTGACC 
TATATGAGTT CCGGAATGCT CGGTGCAACA CAGTTTATGC CACAAAATTC AAGTCATCCA 
TCAACGTCTG TAATGATGCA ACAGGTACCA CCACAAAACG GTGGTGCAAC ACGTTCATCA 
CCAACTGAGA TGCAACAGTG TATGCAGGCA ATGTCAGAAG ACTCGATAGA AATGCGGGAT 
TATAATTCTG GAGTTCATCA TATGCATCCT CATCAAATGC AAATGCAACA GCAGCAGCAA 
CATCATCAGC AGCAATACAA TATGTCGTAT CATAATCATC AACAACAAAT GCAGCAGATG 
CATTACCACC AGCAGCAACA GCAATATCAG CAGCAGCAGG CTCAACATCA TCAGATGTAT 
GCTCCACAAA TTCAGCAACA GCAACAGCAG CCCCAGCAAC AGTCACAACA ACAGTCTGCC 
CAACAGCCCC AACAGTCATC GGCTGCGTTA CAACATGTGA ATGAGTCGAG CAATCTCTCG 
TCGGCTGGTA GTATTTCGGA TCGTGAACCA GAGCAACACG GTGGTACCCC TCAACGACCG 
ACAGCGCCAC AGTCGTCGAC AGCCACTGAC AAGAAAACCA GAAAGCGACG AAAAGCTGGT 
CCAACTGAAG ATCAGGCAAC TCCGAAACAA GAAAGGAAAA TCACAGAATT CATGAAAGTT 
GGAGGAGAGG TTGCAAGTGG AAATAGTGTT GCACGATGTC TATTGACGGA ATATCATCAA 
AATCAAGGAT CACCGAAGAG GCAGCCAGCT GTACAGCAGA ATGGATCTAA CAGTTACGAT 
TCACAGCAGC AGCAACCACA AATGAATCAG CATGAAATGC AAAACTCATA TTGGGGAGTT 
GCTACACCGT CATTGGGTGT TAATAATCGT GGAACCCCGA CTCCAACTCA ACAACAACAT 
TATTCATCGG ATTCAAACTC AAACTCGAAT CAATCACCAC CTGGTCAGGG AAATCAATCA 
GGAAGGATGG TGAGAACAAT CGATGAAGAG ACTCAGACGG ATTCTTCGCT ATCTCAGGCT 
AATCCTCAAA ATGCTGATGA AGTCGCCAAA ATGAATAGAA TCATTGAAGA CCATCGTCGC 
CAAATCGAAG AGTTGAACTC GAAGAATAGT TTGGAACGAA GAAAGAATGA AGCTTCGAAA 
GAAACAATCA AGCGATTACT TATCGATAAG AATCAGATCG AACGAAAAGC CCTTCGCGAC 
AAAACGGCTG CCGATTCACC TCGAATTGGA TGTTTCAAAA CTACTCGAAC TGGTGATTCA 
TTCCGTGATC AATGGGTTGA TGGGTGGGCG TTTGCCGAAA TGGATAAGAA AACTGAACAG 
ATCAATGCTG AAAGAAATGA AATTGCAAGT GCTTCTGCCT TGTTGAAAAA ACGAAAGCCT 
CTTGGAATCG GTAAAGAACC TAAACGCCCA CAGGCAGTCA ATTCTCAAAA TGACTCCAAC 
GGTATGCAGC CATCAACAAG TTCCAATACG AATGGAGACG ATGCGATCTT CAGACGACCT 
GAAGAACCGA AAGAAATCCA GTACCAAGAG TACATTGAGC TCGATGAAAT CTATAAACTG 
CGCAGGGAGC ACTTGAGAAA AGAAGAAACT GATCTCTCCA TGGAGAAAGA AAGATTGGAA 
AAGGAGAAGC AGCATCATGT GAGAGAGCTG AAACGAGCTT CGAATGAAAG TGCGTCACAG 
TTCAATGATC ATCGACTCTT GCATAAAAGA TACCTCATGC TGAACTTGCT CGGTAAGGGT 
GGATTCAGTG AGGTCTGGAA GGCGTTCGAC ATTGAAGAGA ACCGGTATGT CGCATGCAAG 
ATCCATCATG TTAACAAAGA CTGGAAGGAG GAGAAGAAGG CGAACTACGT CAAGCACGCG 
ATGCGTGAAA AAGACATCCA CAAGTCATTG GATCATTGCC GAATTGTGAA GCAATACGAT 
CTGCTCACAA TCGACAATCA CTCGTTCTGC ACGGTTCTCG AATATGTGCC GGGCAACGAC 
CTCGATTTCT ACCTCAAGCA GAACAGGTCG ATCAGTGAGA AGGAGGCTCG AAGCATTATT 
ATGCAAGTTG TTTCTGCTCT TGTCTATCTG AATGAAAAGA GCACTCCCAT CATTCATTAT 
GATCTAAAGC CTGCGAATAT TCTTCTCGAG TCAGGAAACA CCAGCGGTGC AATCAAGATC 
ACCGACTTTG GGCTGTCGAA AATCATGGAG GGAGAGAGTG ACGATCATGA CTTGGGAATC 
GAGCTCACCT CACAGTTCGC TGGAACTTAT TGGTATCTTC CACCTGAGAC CTTCATCGTT 
CCACCACCAA AGATCACTTG TAAAGTCGAT GTCTGGAGTA TTGGAGTCAT CTTCTATCAA 
TGTATCTATG GAAAGAAGCC GTTCGGAAAT GATCTGACTC AACAAAAGAT CCTCGAATAC 
AATACGATTA TTAATGCAAG AGAAGTGTCA TTCCCAAGCA AGCCTCAGGT GAGCTCCGCT 
GCTCAAGATT TCATAAGAAG ATGCCTGCAG TACAGGAAAG AAGACAGAGC AGATGTTTTC 
GAGCTGGCGA AGCATGAGCT TTTCAGACCA AGAGGAGCGA TAAGAGCCTC CGTTGCCGGA 
TCCCTCTTAC TTGTAATAAT CACGTTGTGT CAACATTCGA ACAGATCCGA AGACCGTTTG 
TGTGTGAGCA ACGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_871642.2
CDS	119..3016
Translation	MSMLSMDGIVAGGGSSSGGGERSFVLEQKMFNTGPQNKALPTVQSSGSSSNHAPIVGESPLGTVSSTMATGDTGRVGNVTYMSSGMLGATQFMPQNSSHPSTSVMMQQVPPQNGGATRSSPTEMQQCMQAMSEDSIEMRDYNSGVHHMHPHQMQMQQQQQHHQQQYNMSYHNHQQQMQQMHYHQQQQQYQQQQAQHHQMYAPQIQQQQQQPQQQSQQQSAQQPQQSSAALQHVNESSNLSSAGSISDREPEQHGGTPQRPTAPQSSTATDKKTRKRRKAGPTEDQATPKQERKITEFMKVGGEVASGNSVARCLLTEYHQNQGSPKRQPAVQQNGSNSYDSQQQQPQMNQHEMQNSYWGVATPSLGVNNRGTPTPTQQQHYSSDSNSNSNQSPPGQGNQSGRMVRTIDEETQTDSSLSQANPQNADEVAKMNRIIEDHRRQIEELNSKNSLERRKNEASKETIKRLLIDKNQIERKALRDKTAADSPRIGCFKTTRTGDSFRDQWVDGWAFAEMDKKTEQINAERNEIASASALLKKRKPLGIGKEPKRPQAVNSQNDSNGMQPSTSSNTNGDDAIFRRPEEPKEIQYQEYIELDEIYKLRREHLRKEETDLSMEKERLEKEKQHHVRELKRASNESASQFNDHRLLHKRYLMLNLLGKGGFSEVWKAFDIEENRYVACKIHHVNKDWKEEKKANYVKHAMREKDIHKSLDHCRIVKQYDLLTIDNHSFCTVLEYVPGNDLDFYLKQNRSISEKEARSIIMQVVSALVYLNEKSTPIIHYDLKPANILLESGNTSGAIKITDFGLSKIMEGESDDHDLGIELTSQFAGTYWYLPPETFIVPPPKITCKVDVWSIGVIFYQCIYGKKPFGNDLTQQKILEYNTIINAREVSFPSKPQVSSAAQDFIRRCLQYRKEDRADVFELAKHELFRPRGAIRASVAGSLLLVIITLCQHSNRSEDRLCVSNV

Target ORF information:

RefSeq Version	NM_181913.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181913.4

ORF Insert Sequence:

ATGTCGATGT TGTCAATGGA CGGTATCGTC GCAGGAGGCG GAAGCAGCAG TGGTGGCGGA 
GAACGAAGTT TCGTATTAGA GCAAAAGATG TTCAATACGG GCCCACAGAA CAAAGCTTTA 
CCAACGGTCC AGAGCAGCGG TTCATCATCG AATCACGCTC CGATCGTCGG CGAGTCTCCA 
CTCGGCACTG TGAGCAGCAC AATGGCTACC GGTGATACGG GTCGTGTTGG AAACGTGACC 
TATATGAGTT CCGGAATGCT CGGTGCAACA CAGTTTATGC CACAAAATTC AAGTCATCCA 
TCAACGTCTG TAATGATGCA ACAGGTACCA CCACAAAACG GTGGTGCAAC ACGTTCATCA 
CCAACTGAGA TGCAACAGTG TATGCAGGCA ATGTCAGAAG ACTCGATAGA AATGCGGGAT 
TATAATTCTG GAGTTCATCA TATGCATCCT CATCAAATGC AAATGCAACA GCAGCAGCAA 
CATCATCAGC AGCAATACAA TATGTCGTAT CATAATCATC AACAACAAAT GCAGCAGATG 
CATTACCACC AGCAGCAACA GCAATATCAG CAGCAGCAGG CTCAACATCA TCAGATGTAT 
GCTCCACAAA TTCAGCAACA GCAACAGCAG CCCCAGCAAC AGTCACAACA ACAGTCTGCC 
CAACAGCCCC AACAGTCATC GGCTGCGTTA CAACATGTGA ATGAGTCGAG CAATCTCTCG 
TCGGCTGGTA GTATTTCGGA TCGTGAACCA GAGCAACACG GTGGTACCCC TCAACGACCG 
ACAGCGCCAC AGTCGTCGAC AGCCACTGAC AAGAAAACCA GAAAGCGACG AAAAGCTGGT 
CCAACTGAAG ATCAGGCAAC TCCGAAACAA GAAAGGAAAA TCACAGAATT CATGAAAGTT 
GGAGGAGAGG TTGCAAGTGG AAATAGTGTT GCACGATGTC TATTGACGGA ATATCATCAA 
AATCAAGGAT CACCGAAGAG GCAGCCAGCT GTACAGCAGA ATGGATCTAA CAGTTACGAT 
TCACAGCAGC AGCAACCACA AATGAATCAG CATGAAATGC AAAACTCATA TTGGGGAGTT 
GCTACACCGT CATTGGGTGT TAATAATCGT GGAACCCCGA CTCCAACTCA ACAACAACAT 
TATTCATCGG ATTCAAACTC AAACTCGAAT CAATCACCAC CTGGTCAGGG AAATCAATCA 
GGAAGGATGG TGAGAACAAT CGATGAAGAG ACTCAGACGG ATTCTTCGCT ATCTCAGGCT 
AATCCTCAAA ATGCTGATGA AGTCGCCAAA ATGAATAGAA TCATTGAAGA CCATCGTCGC 
CAAATCGAAG AGTTGAACTC GAAGAATAGT TTGGAACGAA GAAAGAATGA AGCTTCGAAA 
GAAACAATCA AGCGATTACT TATCGATAAG AATCAGATCG AACGAAAAGC CCTTCGCGAC 
AAAACGGCTG CCGATTCACC TCGAATTGGA TGTTTCAAAA CTACTCGAAC TGGTGATTCA 
TTCCGTGATC AATGGGTTGA TGGGTGGGCG TTTGCCGAAA TGGATAAGAA AACTGAACAG 
ATCAATGCTG AAAGAAATGA AATTGCAAGT GCTTCTGCCT TGTTGAAAAA ACGAAAGCCT 
CTTGGAATCG GTAAAGAACC TAAACGCCCA CAGGCAGTCA ATTCTCAAAA TGACTCCAAC 
GGTATGCAGC CATCAACAAG TTCCAATACG AATGGAGACG ATGCGATCTT CAGACGACCT 
GAAGAACCGA AAGAAATCCA GTACCAAGAG TACATTGAGC TCGATGAAAT CTATAAACTG 
CGCAGGGAGC ACTTGAGAAA AGAAGAAACT GATCTCTCCA TGGAGAAAGA AAGATTGGAA 
AAGGAGAAGC AGCATCATGT GAGAGAGCTG AAACGAGCTT CGAATGAAAG TGCGTCACAG 
TTCAATGATC ATCGACTCTT GCATAAAAGA TACCTCATGC TGAACTTGCT CGGTAAGGGT 
GGATTCAGTG AGGTCTGGAA GGCGTTCGAC ATTGAAGAGA ACCGGTATGT CGCATGCAAG 
ATCCATCATG TTAACAAAGA CTGGAAGGAG GAGAAGAAGG CGAACTACGT CAAGCACGCG 
ATGCGTGAAA AAGACATCCA CAAGTCATTG GATCATTGCC GAATTGTGAA GCAATACGAT 
CTGCTCACAA TCGACAATCA CTCGTTCTGC ACGGTTCTCG AATATGTGCC GGGCAACGAC 
CTCGATTTCT ACCTCAAGCA GAACAGGTCG ATCAGTGAGA AGGAGGCTCG AAGCATTATT 
ATGCAAGTTG TTTCTGCTCT TGTCTATCTG AATGAAAAGA GCACTCCCAT CATTCATTAT 
GATCTAAAGC CTGCGAATAT TCTTCTCGAG TCAGGAAACA CCAGCGGTGC AATCAAGATC 
ACCGACTTTG GGCTGTCGAA AATCATGGAG GGAGAGAGTG ACGATCATGA CTTGGGAATC 
GAGCTCACCT CACAGTTCGC TGGAACTTAT TGGTATCTTC CACCTGAGAC CTTCATCGTT 
CCACCACCAA AGATCACTTG TAAAGTCGAT GTCTGGAGTA TTGGAGTCAT CTTCTATCAA 
TGTATCTATG GAAAGAAGCC GTTCGGAAAT GATCTGACTC AACAAAAGAT CCTCGAATAC 
AATACGATTA TTAATGCAAG AGAAGTGTCA TTCCCAAGCA AGCCTCAGGT GAGCTCCGCT 
GCTCAAGATT TCATAAGAAG ATGCCTGCAG TACAGGAAAG AAGACAGAGC AGATGTTTTC 
GAGCTGGCGA AGCATGAGCT TTTCAGACCA AGAGGAGCGA TAAGAGCCTC CGTTGCCGGA 
TCCCTCTTAC TTGTAATAAT CACGTTGTGT CAACATTCGA ACAGATCCGA AGACCGTTTG 
TGTGTGAGCA ACGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tlk-1 ( NM_001268088.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255017.1 Caenorhabditis elegans Protein kinase domain-containing protein (tlk-1), mRNA.

Price & Availability↑

CloneID	OCa340531
Clone ID Related Accession (Same CDS sequence)	NM_001268088.2 , NM_001268088.1
Accession Version	NM_001268088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein kinase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001268088.1.

ATGAGTTCCG GAATGCTCGG TGCAACACAG TTTATGCCAC AAAATTCAAG TCATCCATCA 
ACGTCTGTAA TGATGCAACA GGTACCACCA CAAAACGGTG GTGCAACACG TTCATCACCA 
ACTGAGATGC AACAGTGTAT GCAGGCAATG TCAGAAGACT CGATAGAAAT GCGGGATTAT 
AATTCTGGAG TTCATCATAT GCATCCTCAT CAAATGCAAA TGCAACAGCA GCAGCAACAT 
CATCAGCAGC AATACAATAT GTCGTATCAT AATCATCAAC AACAAATGCA GCAGATGCAT 
TACCACCAGC AGCAACAGCA ATATCAGCAG CAGCAGGCTC AACATCATCA GATGTATGCT 
CCACAAATTC AGCAACAGCA ACAGCAGCCC CAGCAACAGT CACAACAACA GTCTGCCCAA 
CAGCCCCAAC AGTCATCGGC TGCGTTACAA CATGTGAATG AGTCGAGCAA TCTCTCGTCG 
GCTGGTAGTA TTTCGGATCG TGAACCAGAG CAACACGGTG GTACCCCTCA ACGACCGACA 
GCGCCACAGT CGTCGACAGC CACTGACAAG AAAACCAGAA AGCGACGAAA AGCTGGTCCA 
ACTGAAGATC AGGCAACTCC GAAACAAGAA AGGAAAATCA CAGAATTCAT GAAAGTTGGA 
GGAGAGGTTG CAAGTGGAAA TAGTGTTGCA CGATGTCTAT TGACGGAATA TCATCAAAAT 
CAAGGATCAC CGAAGAGGCA GCCAGCTGTA CAGCAGAATG GATCTAACAG TTACGATTCA 
CAGCAGCAGC AACCACAAAT GAATCAGCAT GAAATGCAAA ACTCATATTG GGGAGTTGCT 
ACACCGTCAT TGGGTGTTAA TAATCGTGGA ACCCCGACTC CAACTCAACA ACAACATTAT 
TCATCGGATT CAAACTCAAA CTCGAATCAA TCACCACCTG GTCAGGGAAA TCAATCAGGA 
AGGATGGTGA GAACAATCGA TGAAGAGACT CAGACGGATT CTTCGCTATC TCAGGCTAAT 
CCTCAAAATG CTGATGAAGT CGCCAAAATG AATAGAATCA TTGAAGACCA TCGTCGCCAA 
ATCGAAGAGT TGAACTCGAA GAATAGTTTG GAACGAAGAA AGAATGAAGC TTCGAAAGAA 
ACAATCAAGC GATTACTTAT CGATAAGAAT CAGATCGAAC GAAAAGCCCT TCGCGACAAA 
ACGGCTGCCG ATTCACCTCG AATTGGATGT TTCAAAACTA CTCGAACTGG TGATTCATTC 
CGTGATCAAT GGGTTGATGG GTGGGCGTTT GCCGAAATGG ATAAGAAAAC TGAACAGATC 
AATGCTGAAA GAAATGAAAT TGCAAGTGCT TCTGCCTTGT TGAAAAAACG AAAGCCTCTT 
GGAATCGGTA AAGAACCTAA ACGCCCACAG GCAGTCAATT CTCAAAATGA CTCCAACGGT 
ATGCAGCCAT CAACAAGTTC CAATACGAAT GGAGACGATG CGATCTTCAG ACGACCTGAA 
GAACCGAAAG AAATCCAGTA CCAAGAGTAC ATTGAGCTCG ATGAAATCTA TAAACTGCGC 
AGGGAGCACT TGAGAAAAGA AGAAACTGAT CTCTCCATGG AGAAAGAAAG ATTGGAAAAG 
GAGAAGCAGC ATCATGTGAG AGAGCTGAAA CGAGCTTCGA ATGAAAGTGC GTCACAGTTC 
AATGATCATC GACTCTTGCA TAAAAGATAC CTCATGCTGA ACTTGCTCGG TAAGGGTGGA 
TTCAGTGAGG TCTGGAAGGC GTTCGACATT GAAGAGAACC GGTATGTCGC ATGCAAGATC 
CATCATGTTA ACAAAGACTG GAAGGAGGAG AAGAAGGCGA ACTACGTCAA GCACGCGATG 
CGTGAAAAAG ACATCCACAA GTCATTGGAT CATTGCCGAA TTGTGAAGCA ATACGATCTG 
CTCACAATCG ACAATCACTC GTTCTGCACG GTTCTCGAAT ATGTGCCGGG CAACGACCTC 
GATTTCTACC TCAAGCAGAA CAGGTCGATC AGTGAGAAGG AGGCTCGAAG CATTATTATG 
CAAGTTGTTT CTGCTCTTGT CTATCTGAAT GAAAAGAGCA CTCCCATCAT TCATTATGAT 
CTAAAGCCTG CGAATATTCT TCTCGAGTCA GGAAACACCA GCGGTGCAAT CAAGATCACC 
GACTTTGGGC TGTCGAAAAT CATGGAGGGA GAGAGTGACG ATCATGACTT GGGAATCGAG 
CTCACCTCAC AGTTCGCTGG AACTTATTGG TATCTTCCAC CTGAGACCTT CATCGTTCCA 
CCACCAAAGA TCACTTGTAA AGTCGATGTC TGGAGTATTG GAGTCATCTT CTATCAATGT 
ATCTATGGAA AGAAGCCGTT CGGAAATGAT CTGACTCAAC AAAAGATCCT CGAATACAAT 
ACGATTATTA ATGCAAGAGA AGTGTCATTC CCAAGCAAGC CTCAGGTGAG CTCCGCTGCT 
CAAGATTTCA TAAGAAGATG CCTGCAGTAC AGGAAAGAAG ACAGAGCAGA TGTTTTCGAG 
CTGGCGAAGC ATGAGCTTTT CAGACCAAGA GGAGCGATAA GAGCCTCCGT TGCCGGATCC 
CTCTTACTTG TAATAATCAC GTTGTGTCAA CATTCGAACA GATCCGAAGA CCGTTTGTGT 
GTGAGCAACG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255017.1
CDS	5..2659
Translation	MSSGMLGATQFMPQNSSHPSTSVMMQQVPPQNGGATRSSPTEMQQCMQAMSEDSIEMRDYNSGVHHMHPHQMQMQQQQQHHQQQYNMSYHNHQQQMQQMHYHQQQQQYQQQQAQHHQMYAPQIQQQQQQPQQQSQQQSAQQPQQSSAALQHVNESSNLSSAGSISDREPEQHGGTPQRPTAPQSSTATDKKTRKRRKAGPTEDQATPKQERKITEFMKVGGEVASGNSVARCLLTEYHQNQGSPKRQPAVQQNGSNSYDSQQQQPQMNQHEMQNSYWGVATPSLGVNNRGTPTPTQQQHYSSDSNSNSNQSPPGQGNQSGRMVRTIDEETQTDSSLSQANPQNADEVAKMNRIIEDHRRQIEELNSKNSLERRKNEASKETIKRLLIDKNQIERKALRDKTAADSPRIGCFKTTRTGDSFRDQWVDGWAFAEMDKKTEQINAERNEIASASALLKKRKPLGIGKEPKRPQAVNSQNDSNGMQPSTSSNTNGDDAIFRRPEEPKEIQYQEYIELDEIYKLRREHLRKEETDLSMEKERLEKEKQHHVRELKRASNESASQFNDHRLLHKRYLMLNLLGKGGFSEVWKAFDIEENRYVACKIHHVNKDWKEEKKANYVKHAMREKDIHKSLDHCRIVKQYDLLTIDNHSFCTVLEYVPGNDLDFYLKQNRSISEKEARSIIMQVVSALVYLNEKSTPIIHYDLKPANILLESGNTSGAIKITDFGLSKIMEGESDDHDLGIELTSQFAGTYWYLPPETFIVPPPKITCKVDVWSIGVIFYQCIYGKKPFGNDLTQQKILEYNTIINAREVSFPSKPQVSSAAQDFIRRCLQYRKEDRADVFELAKHELFRPRGAIRASVAGSLLLVIITLCQHSNRSEDRLCVSNV

Target ORF information:

RefSeq Version	NM_001268088.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein kinase domain-containing protein (tlk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268088.2

ORF Insert Sequence:

ATGAGTTCCG GAATGCTCGG TGCAACACAG TTTATGCCAC AAAATTCAAG TCATCCATCA 
ACGTCTGTAA TGATGCAACA GGTACCACCA CAAAACGGTG GTGCAACACG TTCATCACCA 
ACTGAGATGC AACAGTGTAT GCAGGCAATG TCAGAAGACT CGATAGAAAT GCGGGATTAT 
AATTCTGGAG TTCATCATAT GCATCCTCAT CAAATGCAAA TGCAACAGCA GCAGCAACAT 
CATCAGCAGC AATACAATAT GTCGTATCAT AATCATCAAC AACAAATGCA GCAGATGCAT 
TACCACCAGC AGCAACAGCA ATATCAGCAG CAGCAGGCTC AACATCATCA GATGTATGCT 
CCACAAATTC AGCAACAGCA ACAGCAGCCC CAGCAACAGT CACAACAACA GTCTGCCCAA 
CAGCCCCAAC AGTCATCGGC TGCGTTACAA CATGTGAATG AGTCGAGCAA TCTCTCGTCG 
GCTGGTAGTA TTTCGGATCG TGAACCAGAG CAACACGGTG GTACCCCTCA ACGACCGACA 
GCGCCACAGT CGTCGACAGC CACTGACAAG AAAACCAGAA AGCGACGAAA AGCTGGTCCA 
ACTGAAGATC AGGCAACTCC GAAACAAGAA AGGAAAATCA CAGAATTCAT GAAAGTTGGA 
GGAGAGGTTG CAAGTGGAAA TAGTGTTGCA CGATGTCTAT TGACGGAATA TCATCAAAAT 
CAAGGATCAC CGAAGAGGCA GCCAGCTGTA CAGCAGAATG GATCTAACAG TTACGATTCA 
CAGCAGCAGC AACCACAAAT GAATCAGCAT GAAATGCAAA ACTCATATTG GGGAGTTGCT 
ACACCGTCAT TGGGTGTTAA TAATCGTGGA ACCCCGACTC CAACTCAACA ACAACATTAT 
TCATCGGATT CAAACTCAAA CTCGAATCAA TCACCACCTG GTCAGGGAAA TCAATCAGGA 
AGGATGGTGA GAACAATCGA TGAAGAGACT CAGACGGATT CTTCGCTATC TCAGGCTAAT 
CCTCAAAATG CTGATGAAGT CGCCAAAATG AATAGAATCA TTGAAGACCA TCGTCGCCAA 
ATCGAAGAGT TGAACTCGAA GAATAGTTTG GAACGAAGAA AGAATGAAGC TTCGAAAGAA 
ACAATCAAGC GATTACTTAT CGATAAGAAT CAGATCGAAC GAAAAGCCCT TCGCGACAAA 
ACGGCTGCCG ATTCACCTCG AATTGGATGT TTCAAAACTA CTCGAACTGG TGATTCATTC 
CGTGATCAAT GGGTTGATGG GTGGGCGTTT GCCGAAATGG ATAAGAAAAC TGAACAGATC 
AATGCTGAAA GAAATGAAAT TGCAAGTGCT TCTGCCTTGT TGAAAAAACG AAAGCCTCTT 
GGAATCGGTA AAGAACCTAA ACGCCCACAG GCAGTCAATT CTCAAAATGA CTCCAACGGT 
ATGCAGCCAT CAACAAGTTC CAATACGAAT GGAGACGATG CGATCTTCAG ACGACCTGAA 
GAACCGAAAG AAATCCAGTA CCAAGAGTAC ATTGAGCTCG ATGAAATCTA TAAACTGCGC 
AGGGAGCACT TGAGAAAAGA AGAAACTGAT CTCTCCATGG AGAAAGAAAG ATTGGAAAAG 
GAGAAGCAGC ATCATGTGAG AGAGCTGAAA CGAGCTTCGA ATGAAAGTGC GTCACAGTTC 
AATGATCATC GACTCTTGCA TAAAAGATAC CTCATGCTGA ACTTGCTCGG TAAGGGTGGA 
TTCAGTGAGG TCTGGAAGGC GTTCGACATT GAAGAGAACC GGTATGTCGC ATGCAAGATC 
CATCATGTTA ACAAAGACTG GAAGGAGGAG AAGAAGGCGA ACTACGTCAA GCACGCGATG 
CGTGAAAAAG ACATCCACAA GTCATTGGAT CATTGCCGAA TTGTGAAGCA ATACGATCTG 
CTCACAATCG ACAATCACTC GTTCTGCACG GTTCTCGAAT ATGTGCCGGG CAACGACCTC 
GATTTCTACC TCAAGCAGAA CAGGTCGATC AGTGAGAAGG AGGCTCGAAG CATTATTATG 
CAAGTTGTTT CTGCTCTTGT CTATCTGAAT GAAAAGAGCA CTCCCATCAT TCATTATGAT 
CTAAAGCCTG CGAATATTCT TCTCGAGTCA GGAAACACCA GCGGTGCAAT CAAGATCACC 
GACTTTGGGC TGTCGAAAAT CATGGAGGGA GAGAGTGACG ATCATGACTT GGGAATCGAG 
CTCACCTCAC AGTTCGCTGG AACTTATTGG TATCTTCCAC CTGAGACCTT CATCGTTCCA 
CCACCAAAGA TCACTTGTAA AGTCGATGTC TGGAGTATTG GAGTCATCTT CTATCAATGT 
ATCTATGGAA AGAAGCCGTT CGGAAATGAT CTGACTCAAC AAAAGATCCT CGAATACAAT 
ACGATTATTA ATGCAAGAGA AGTGTCATTC CCAAGCAAGC CTCAGGTGAG CTCCGCTGCT 
CAAGATTTCA TAAGAAGATG CCTGCAGTAC AGGAAAGAAG ACAGAGCAGA TGTTTTCGAG 
CTGGCGAAGC ATGAGCTTTT CAGACCAAGA GGAGCGATAA GAGCCTCCGT TGCCGGATCC 
CTCTTACTTG TAATAATCAC GTTGTGTCAA CATTCGAACA GATCCGAAGA CCGTTTGTGT 
GTGAGCAACG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tlk-1 ( NM_001047379.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001040844.1 Caenorhabditis elegans Protein kinase domain-containing protein (tlk-1), mRNA.

Price & Availability↑

CloneID	OCa340577
Clone ID Related Accession (Same CDS sequence)	NM_001047379.1 , NM_001047379.2
Accession Version	NM_001047379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein kinase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001047379.1.

ATGGCTACCG GTGATACGGG TCGTGTTGGA AACGTGACCT ATATGAGTTC CGGAATGCTC 
GGTGCAACAC AGTTTATGCC ACAAAATTCA AGTCATCCAT CAACGTCTGT AATGATGCAA 
CAGGTACCAC CACAAAACGG TGGTGCAACA CGTTCATCAC CAACTGAGAT GCAACAGTGT 
ATGCAGGCAA TGTCAGAAGA CTCGATAGAA ATGCGGGATT ATAATTCTGG AGTTCATCAT 
ATGCATCCTC ATCAAATGCA AATGCAACAG CAGCAGCAAC ATCATCAGCA GCAATACAAT 
ATGTCGTATC ATAATCATCA ACAACAAATG CAGCAGATGC ATTACCACCA GCAGCAACAG 
CAATATCAGC AGCAGCAGGC TCAACATCAT CAGATGTATG CTCCACAAAT TCAGCAACAG 
CAACAGCAGC CCCAGCAACA GTCACAACAA CAGTCTGCCC AACAGCCCCA ACAGTCATCG 
GCTGCGTTAC AACATGTGAA TGAGTCGAGC AATCTCTCGT CGGCTGGTAG TATTTCGGAT 
CGTGAACCAG AGCAACACGG TGGTACCCCT CAACGACCGA CAGCGCCACA GTCGTCGACA 
GCCACTGACA AGAAAACCAG AAAGCGACGA AAAGCTGGTC CAACTGAAGA TCAGGCAACT 
CCGAAACAAG AAAGGAAAAT CACAGAATTC ATGAAAGTTG GAGGAGAGGT TGCAAGTGGA 
AATAGTGTTG CACGATGTCT ATTGACGGAA TATCATCAAA ATCAAGGATC ACCGAAGAGG 
CAGCCAGCTG TACAGCAGAA TGGATCTAAC AGTTACGATT CACAGCAGCA GCAACCACAA 
ATGAATCAGC ATGAAATGCA AAACTCATAT TGGGGAGTTG CTACACCGTC ATTGGGTGTT 
AATAATCGTG GAACCCCGAC TCCAACTCAA CAACAACATT ATTCATCGGA TTCAAACTCA 
AACTCGAATC AATCACCACC TGGTCAGGGA AATCAATCAG GAAGGATGGT GAGAACAATC 
GATGAAGAGA CTCAGACGGA TTCTTCGCTA TCTCAGGCTA ATCCTCAAAA TGCTGATGAA 
GTCGCCAAAA TGAATAGAAT CATTGAAGAC CATCGTCGCC AAATCGAAGA GTTGAACTCG 
AAGAATAGTT TGGAACGAAG AAAGAATGAA GCTTCGAAAG AAACAATCAA GCGATTACTT 
ATCGATAAGA ATCAGATCGA ACGAAAAGCC CTTCGCGACA AAACGGCTGC CGATTCACCT 
CGAATTGGAT GTTTCAAAAC TACTCGAACT GGTGATTCAT TCCGTGATCA ATGGGTTGAT 
GGGTGGGCGT TTGCCGAAAT GGATAAGAAA ACTGAACAGA TCAATGCTGA AAGAAATGAA 
ATTGCAAGTG CTTCTGCCTT GTTGAAAAAA CGAAAGCCTC TTGGAATCGG TAAAGAACCT 
AAACGCCCAC AGGCAGTCAA TTCTCAAAAT GACTCCAACG GTATGCAGCC ATCAACAAGT 
TCCAATACGA ATGGAGACGA TGCGATCTTC AGACGACCTG AAGAACCGAA AGAAATCCAG 
TACCAAGAGT ACATTGAGCT CGATGAAATC TATAAACTGC GCAGGGAGCA CTTGAGAAAA 
GAAGAAACTG ATCTCTCCAT GGAGAAAGAA AGATTGGAAA AGGAGAAGCA GCATCATGTG 
AGAGAGCTGA AACGAGCTTC GAATGAAAGT GCGTCACAGT TCAATGATCA TCGACTCTTG 
CATAAAAGAT ACCTCATGCT GAACTTGCTC GGTAAGGGTG GATTCAGTGA GGTCTGGAAG 
GCGTTCGACA TTGAAGAGAA CCGGTATGTC GCATGCAAGA TCCATCATGT TAACAAAGAC 
TGGAAGGAGG AGAAGAAGGC GAACTACGTC AAGCACGCGA TGCGTGAAAA AGACATCCAC 
AAGTCATTGG ATCATTGCCG AATTGTGAAG CAATACGATC TGCTCACAAT CGACAATCAC 
TCGTTCTGCA CGGTTCTCGA ATATGTGCCG GGCAACGACC TCGATTTCTA CCTCAAGCAG 
AACAGGTCGA TCAGTGAGAA GGAGGCTCGA AGCATTATTA TGCAAGTTGT TTCTGCTCTT 
GTCTATCTGA ATGAAAAGAG CACTCCCATC ATTCATTATG ATCTAAAGCC TGCGAATATT 
CTTCTCGAGT CAGGAAACAC CAGCGGTGCA ATCAAGATCA CCGACTTTGG GCTGTCGAAA 
ATCATGGAGG GAGAGAGTGA CGATCATGAC TTGGGAATCG AGCTCACCTC ACAGTTCGCT 
GGAACTTATT GGTATCTTCC ACCTGAGACC TTCATCGTTC CACCACCAAA GATCACTTGT 
AAAGTCGATG TCTGGAGTAT TGGAGTCATC TTCTATCAAT GTATCTATGG AAAGAAGCCG 
TTCGGAAATG ATCTGACTCA ACAAAAGATC CTCGAATACA ATACGATTAT TAATGCAAGA 
GAAGTGTCAT TCCCAAGCAA GCCTCAGGTG AGCTCCGCTG CTCAAGATTT CATAAGAAGA 
TGCCTGCAGT ACAGGAAAGA AGACAGAGCA GATGTTTTCG AGCTGGCGAA GCATGAGCTT 
TTCAGACCAA GAGGAGCGAT AAGAGCCTCC GTTGCCGGAT CCGTAAGTTC TCCAAGCATT 
CCACGCAGTC CATCAGTGAA TCGTGAAGAT GACAATATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001040844.1
CDS	314..2995
Translation	MATGDTGRVGNVTYMSSGMLGATQFMPQNSSHPSTSVMMQQVPPQNGGATRSSPTEMQQCMQAMSEDSIEMRDYNSGVHHMHPHQMQMQQQQQHHQQQYNMSYHNHQQQMQQMHYHQQQQQYQQQQAQHHQMYAPQIQQQQQQPQQQSQQQSAQQPQQSSAALQHVNESSNLSSAGSISDREPEQHGGTPQRPTAPQSSTATDKKTRKRRKAGPTEDQATPKQERKITEFMKVGGEVASGNSVARCLLTEYHQNQGSPKRQPAVQQNGSNSYDSQQQQPQMNQHEMQNSYWGVATPSLGVNNRGTPTPTQQQHYSSDSNSNSNQSPPGQGNQSGRMVRTIDEETQTDSSLSQANPQNADEVAKMNRIIEDHRRQIEELNSKNSLERRKNEASKETIKRLLIDKNQIERKALRDKTAADSPRIGCFKTTRTGDSFRDQWVDGWAFAEMDKKTEQINAERNEIASASALLKKRKPLGIGKEPKRPQAVNSQNDSNGMQPSTSSNTNGDDAIFRRPEEPKEIQYQEYIELDEIYKLRREHLRKEETDLSMEKERLEKEKQHHVRELKRASNESASQFNDHRLLHKRYLMLNLLGKGGFSEVWKAFDIEENRYVACKIHHVNKDWKEEKKANYVKHAMREKDIHKSLDHCRIVKQYDLLTIDNHSFCTVLEYVPGNDLDFYLKQNRSISEKEARSIIMQVVSALVYLNEKSTPIIHYDLKPANILLESGNTSGAIKITDFGLSKIMEGESDDHDLGIELTSQFAGTYWYLPPETFIVPPPKITCKVDVWSIGVIFYQCIYGKKPFGNDLTQQKILEYNTIINAREVSFPSKPQVSSAAQDFIRRCLQYRKEDRADVFELAKHELFRPRGAIRASVAGSVSSPSIPRSPSVNREDDNM

Target ORF information:

RefSeq Version	NM_001047379.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein kinase domain-containing protein (tlk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047379.2

ORF Insert Sequence:

ATGGCTACCG GTGATACGGG TCGTGTTGGA AACGTGACCT ATATGAGTTC CGGAATGCTC 
GGTGCAACAC AGTTTATGCC ACAAAATTCA AGTCATCCAT CAACGTCTGT AATGATGCAA 
CAGGTACCAC CACAAAACGG TGGTGCAACA CGTTCATCAC CAACTGAGAT GCAACAGTGT 
ATGCAGGCAA TGTCAGAAGA CTCGATAGAA ATGCGGGATT ATAATTCTGG AGTTCATCAT 
ATGCATCCTC ATCAAATGCA AATGCAACAG CAGCAGCAAC ATCATCAGCA GCAATACAAT 
ATGTCGTATC ATAATCATCA ACAACAAATG CAGCAGATGC ATTACCACCA GCAGCAACAG 
CAATATCAGC AGCAGCAGGC TCAACATCAT CAGATGTATG CTCCACAAAT TCAGCAACAG 
CAACAGCAGC CCCAGCAACA GTCACAACAA CAGTCTGCCC AACAGCCCCA ACAGTCATCG 
GCTGCGTTAC AACATGTGAA TGAGTCGAGC AATCTCTCGT CGGCTGGTAG TATTTCGGAT 
CGTGAACCAG AGCAACACGG TGGTACCCCT CAACGACCGA CAGCGCCACA GTCGTCGACA 
GCCACTGACA AGAAAACCAG AAAGCGACGA AAAGCTGGTC CAACTGAAGA TCAGGCAACT 
CCGAAACAAG AAAGGAAAAT CACAGAATTC ATGAAAGTTG GAGGAGAGGT TGCAAGTGGA 
AATAGTGTTG CACGATGTCT ATTGACGGAA TATCATCAAA ATCAAGGATC ACCGAAGAGG 
CAGCCAGCTG TACAGCAGAA TGGATCTAAC AGTTACGATT CACAGCAGCA GCAACCACAA 
ATGAATCAGC ATGAAATGCA AAACTCATAT TGGGGAGTTG CTACACCGTC ATTGGGTGTT 
AATAATCGTG GAACCCCGAC TCCAACTCAA CAACAACATT ATTCATCGGA TTCAAACTCA 
AACTCGAATC AATCACCACC TGGTCAGGGA AATCAATCAG GAAGGATGGT GAGAACAATC 
GATGAAGAGA CTCAGACGGA TTCTTCGCTA TCTCAGGCTA ATCCTCAAAA TGCTGATGAA 
GTCGCCAAAA TGAATAGAAT CATTGAAGAC CATCGTCGCC AAATCGAAGA GTTGAACTCG 
AAGAATAGTT TGGAACGAAG AAAGAATGAA GCTTCGAAAG AAACAATCAA GCGATTACTT 
ATCGATAAGA ATCAGATCGA ACGAAAAGCC CTTCGCGACA AAACGGCTGC CGATTCACCT 
CGAATTGGAT GTTTCAAAAC TACTCGAACT GGTGATTCAT TCCGTGATCA ATGGGTTGAT 
GGGTGGGCGT TTGCCGAAAT GGATAAGAAA ACTGAACAGA TCAATGCTGA AAGAAATGAA 
ATTGCAAGTG CTTCTGCCTT GTTGAAAAAA CGAAAGCCTC TTGGAATCGG TAAAGAACCT 
AAACGCCCAC AGGCAGTCAA TTCTCAAAAT GACTCCAACG GTATGCAGCC ATCAACAAGT 
TCCAATACGA ATGGAGACGA TGCGATCTTC AGACGACCTG AAGAACCGAA AGAAATCCAG 
TACCAAGAGT ACATTGAGCT CGATGAAATC TATAAACTGC GCAGGGAGCA CTTGAGAAAA 
GAAGAAACTG ATCTCTCCAT GGAGAAAGAA AGATTGGAAA AGGAGAAGCA GCATCATGTG 
AGAGAGCTGA AACGAGCTTC GAATGAAAGT GCGTCACAGT TCAATGATCA TCGACTCTTG 
CATAAAAGAT ACCTCATGCT GAACTTGCTC GGTAAGGGTG GATTCAGTGA GGTCTGGAAG 
GCGTTCGACA TTGAAGAGAA CCGGTATGTC GCATGCAAGA TCCATCATGT TAACAAAGAC 
TGGAAGGAGG AGAAGAAGGC GAACTACGTC AAGCACGCGA TGCGTGAAAA AGACATCCAC 
AAGTCATTGG ATCATTGCCG AATTGTGAAG CAATACGATC TGCTCACAAT CGACAATCAC 
TCGTTCTGCA CGGTTCTCGA ATATGTGCCG GGCAACGACC TCGATTTCTA CCTCAAGCAG 
AACAGGTCGA TCAGTGAGAA GGAGGCTCGA AGCATTATTA TGCAAGTTGT TTCTGCTCTT 
GTCTATCTGA ATGAAAAGAG CACTCCCATC ATTCATTATG ATCTAAAGCC TGCGAATATT 
CTTCTCGAGT CAGGAAACAC CAGCGGTGCA ATCAAGATCA CCGACTTTGG GCTGTCGAAA 
ATCATGGAGG GAGAGAGTGA CGATCATGAC TTGGGAATCG AGCTCACCTC ACAGTTCGCT 
GGAACTTATT GGTATCTTCC ACCTGAGACC TTCATCGTTC CACCACCAAA GATCACTTGT 
AAAGTCGATG TCTGGAGTAT TGGAGTCATC TTCTATCAAT GTATCTATGG AAAGAAGCCG 
TTCGGAAATG ATCTGACTCA ACAAAAGATC CTCGAATACA ATACGATTAT TAATGCAAGA 
GAAGTGTCAT TCCCAAGCAA GCCTCAGGTG AGCTCCGCTG CTCAAGATTT CATAAGAAGA 
TGCCTGCAGT ACAGGAAAGA AGACAGAGCA GATGTTTTCG AGCTGGCGAA GCATGAGCTT 
TTCAGACCAA GAGGAGCGAT AAGAGCCTCC GTTGCCGGAT CCGTAAGTTC TCCAAGCATT 
CCACGCAGTC CATCAGTGAA TCGTGAAGAT GACAATATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tlk-1 ( NM_066744.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499145.2 Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), partial mRNA.

Price & Availability↑

CloneID	OCa340104
Clone ID Related Accession (Same CDS sequence)	NM_066744.4 , NM_066744.3
Accession Version	NM_066744.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase tousled-like 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_066744.2. COMPLETENESS: incomplete on both ends.

ATGTCGATGT TGTCAATGGA CGGTATCGTC GCAGGAGGCG GAAGCAGCAG TGGTGGCGGA 
GAACGAAGTT TCGTATTAGA GCAAAAGATG TTCAATACGG GCCCACAGAA CAAAGCTTTA 
CCAACGGTCC AGAGCAGCGG TTCATCATCG AATCACGCTC CGATCGTCGG CGAGTCTCCA 
CTCGGCACTG TGAGCAGCAC AATGGCTACC GGTGATACGG GTCGTGTTGG AAACGTGACC 
TATATGAGTT CCGGAATGCT CGGTGCAACA CAGTTTATGC CACAAAATTC AAGTCATCCA 
TCAACGTCTG TAATGATGCA ACAGGTACCA CCACAAAACG GTGGTGCAAC ACGTTCATCA 
CCAACTGAGA TGCAACAGTG TATGCAGGCA ATGTCAGAAG ACTCGATAGA AATGCGGGAT 
TATAATTCTG GAGTTCATCA TATGCATCCT CATCAAATGC AAATGCAACA GCAGCAGCAA 
CATCATCAGC AGCAATACAA TATGTCGTAT CATAATCATC AACAACAAAT GCAGCAGATG 
CATTACCACC AGCAGCAACA GCAATATCAG CAGCAGCAGG CTCAACATCA TCAGATGTAT 
GCTCCACAAA TTCAGCAACA GCAACAGCAG CCCCAGCAAC AGTCACAACA ACAGTCTGCC 
CAACAGCCCC AACAGTCATC GGCTGCGTTA CAACATGTGA ATGAGTCGAG CAATCTCTCG 
TCGGCTGGTA GTATTTCGGA TCGTGAACCA GAGCAACACG GTGGTACCCC TCAACGACCG 
ACAGCGCCAC AGTCGTCGAC AGCCACTGAC AAGAAAACCA GAAAGCGACG AAAAGCTGGT 
CCAACTGAAG ATCAGGCAAC TCCGAAACAA GAAAGGAAAA TCACAGAATT CATGAAAGTT 
GGAGGAGAGG TTGCAAGTGG AAATAGTGTT GCACGATGTC TATTGACGGA ATATCATCAA 
AATCAAGGAT CACCGAAGAG GCAGCCAGCT GTACAGCAGA ATGGATCTAA CAGTTACGAT 
TCACAGCAGC AGCAACCACA AATGAATCAG CATGAAATGC AAAACTCATA TTGGGGAGTT 
GCTACACCGT CATTGGGTGT TAATAATCGT GGAACCCCGA CTCCAACTCA ACAACAACAT 
TATTCATCGG ATTCAAACTC AAACTCGAAT CAATCACCAC CTGGTCAGGG AAATCAATCA 
GGAAGGATGG TGAGAACAAT CGATGAAGAG ACTCAGACGG ATTCTTCGCT ATCTCAGGCT 
AATCCTCAAA ATGCTGATGA AGTCGCCAAA ATGAATAGAA TCATTGAAGA CCATCGTCGC 
CAAATCGAAG AGTTGAACTC GAAGAATAGT TTGGAACGAA GAAAGAATGA AGCTTCGAAA 
GAAACAATCA AGCGATTACT TATCGATAAG AATCAGATCG AACGAAAAGC CCTTCGCGAC 
AAAACGGCTG CCGATTCACC TCGAATTGGA TGTTTCAAAA CTACTCGAAC TGGTGATTCA 
TTCCGTGATC AATGGGTTGA TGGGTGGGCG TTTGCCGAAA TGGATAAGAA AACTGAACAG 
ATCAATGCTG AAAGAAATGA AATTGCAAGT GCTTCTGCCT TGTTGAAAAA ACGAAAGCCT 
CTTGGAATCG GTAAAGAACC TAAACGCCCA CAGGCAGTCA ATTCTCAAAA TGACTCCAAC 
GGTATGCAGC CATCAACAAG TTCCAATACG AATGGAGACG ATGCGATCTT CAGACGACCT 
GAAGAACCGA AAGAAATCCA GTACCAAGAG TACATTGAGC TCGATGAAAT CTATAAACTG 
CGCAGGGAGC ACTTGAGAAA AGAAGAAACT GATCTCTCCA TGGAGAAAGA AAGATTGGAA 
AAGGAGAAGC AGCATCATGT GAGAGAGCTG AAACGAGCTT CGAATGAAAG TGCGTCACAG 
TTCAATGATC ATCGACTCTT GCATAAAAGA TACCTCATGC TGAACTTGCT CGGTAAGGGT 
GGATTCAGTG AGGTCTGGAA GGCGTTCGAC ATTGAAGAGA ACCGGTATGT CGCATGCAAG 
ATCCATCATG TTAACAAAGA CTGGAAGGAG GAGAAGAAGG CGAACTACGT CAAGCACGCG 
ATGCGTGAAA AAGACATCCA CAAGTCATTG GATCATTGCC GAATTGTGAA GCAATACGAT 
CTGCTCACAA TCGACAATCA CTCGTTCTGC ACGGTTCTCG AATATGTGCC GGGCAACGAC 
CTCGATTTCT ACCTCAAGCA GAACAGGTCG ATCAGTGAGA AGGAGGCTCG AAGCATTATT 
ATGCAAGTTG TTTCTGCTCT TGTCTATCTG AATGAAAAGA GCACTCCCAT CATTCATTAT 
GATCTAAAGC CTGCGAATAT TCTTCTCGAG TCAGGAAACA CCAGCGGTGC AATCAAGATC 
ACCGACTTTG GGCTGTCGAA AATCATGGAG GGAGAGAGTG ACGATCATGA CTTGGGAATC 
GAGCTCACCT CACAGTTCGC TGGAACTTAT TGGTATCTTC CACCTGAGAC CTTCATCGTT 
CCACCACCAA AGATCACTTG TAAAGTCGAT GTCTGGAGTA TTGGAGTCAT CTTCTATCAA 
TGTATCTATG GAAAGAAGCC GTTCGGAAAT GATCTGACTC AACAAAAGAT CCTCGAATAC 
AATACGATTA TTAATGCAAG AGAAGTGTCA TTCCCAAGCA AGCCTCAGGT GAGCTCCGCT 
GCTCAAGATT TCATAAGAAG ATGCCTGCAG TACAGGAAAG AAGACAGAGC AGATGTTTTC 
GAGCTGGCGA AGCATGAGCT TTTCAGACCA AGAGGAGCGA TAAGAGCCTC CGTTGCCGGA 
TCCGTAAGTT CTCCAAGCAT TCCACGCAGT CCATCAGTGA ATCGTGAAGA TGACAATATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499145.2
CDS	1..2883
Translation	MSMLSMDGIVAGGGSSSGGGERSFVLEQKMFNTGPQNKALPTVQSSGSSSNHAPIVGESPLGTVSSTMATGDTGRVGNVTYMSSGMLGATQFMPQNSSHPSTSVMMQQVPPQNGGATRSSPTEMQQCMQAMSEDSIEMRDYNSGVHHMHPHQMQMQQQQQHHQQQYNMSYHNHQQQMQQMHYHQQQQQYQQQQAQHHQMYAPQIQQQQQQPQQQSQQQSAQQPQQSSAALQHVNESSNLSSAGSISDREPEQHGGTPQRPTAPQSSTATDKKTRKRRKAGPTEDQATPKQERKITEFMKVGGEVASGNSVARCLLTEYHQNQGSPKRQPAVQQNGSNSYDSQQQQPQMNQHEMQNSYWGVATPSLGVNNRGTPTPTQQQHYSSDSNSNSNQSPPGQGNQSGRMVRTIDEETQTDSSLSQANPQNADEVAKMNRIIEDHRRQIEELNSKNSLERRKNEASKETIKRLLIDKNQIERKALRDKTAADSPRIGCFKTTRTGDSFRDQWVDGWAFAEMDKKTEQINAERNEIASASALLKKRKPLGIGKEPKRPQAVNSQNDSNGMQPSTSSNTNGDDAIFRRPEEPKEIQYQEYIELDEIYKLRREHLRKEETDLSMEKERLEKEKQHHVRELKRASNESASQFNDHRLLHKRYLMLNLLGKGGFSEVWKAFDIEENRYVACKIHHVNKDWKEEKKANYVKHAMREKDIHKSLDHCRIVKQYDLLTIDNHSFCTVLEYVPGNDLDFYLKQNRSISEKEARSIIMQVVSALVYLNEKSTPIIHYDLKPANILLESGNTSGAIKITDFGLSKIMEGESDDHDLGIELTSQFAGTYWYLPPETFIVPPPKITCKVDVWSIGVIFYQCIYGKKPFGNDLTQQKILEYNTIINAREVSFPSKPQVSSAAQDFIRRCLQYRKEDRADVFELAKHELFRPRGAIRASVAGSVSSPSIPRSPSVNREDDNM

Target ORF information:

RefSeq Version	NM_066744.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066744.3

ORF Insert Sequence:

ATGTCGATGT TGTCAATGGA CGGTATCGTC GCAGGAGGCG GAAGCAGCAG TGGTGGCGGA 
GAACGAAGTT TCGTATTAGA GCAAAAGATG TTCAATACGG GCCCACAGAA CAAAGCTTTA 
CCAACGGTCC AGAGCAGCGG TTCATCATCG AATCACGCTC CGATCGTCGG CGAGTCTCCA 
CTCGGCACTG TGAGCAGCAC AATGGCTACC GGTGATACGG GTCGTGTTGG AAACGTGACC 
TATATGAGTT CCGGAATGCT CGGTGCAACA CAGTTTATGC CACAAAATTC AAGTCATCCA 
TCAACGTCTG TAATGATGCA ACAGGTACCA CCACAAAACG GTGGTGCAAC ACGTTCATCA 
CCAACTGAGA TGCAACAGTG TATGCAGGCA ATGTCAGAAG ACTCGATAGA AATGCGGGAT 
TATAATTCTG GAGTTCATCA TATGCATCCT CATCAAATGC AAATGCAACA GCAGCAGCAA 
CATCATCAGC AGCAATACAA TATGTCGTAT CATAATCATC AACAACAAAT GCAGCAGATG 
CATTACCACC AGCAGCAACA GCAATATCAG CAGCAGCAGG CTCAACATCA TCAGATGTAT 
GCTCCACAAA TTCAGCAACA GCAACAGCAG CCCCAGCAAC AGTCACAACA ACAGTCTGCC 
CAACAGCCCC AACAGTCATC GGCTGCGTTA CAACATGTGA ATGAGTCGAG CAATCTCTCG 
TCGGCTGGTA GTATTTCGGA TCGTGAACCA GAGCAACACG GTGGTACCCC TCAACGACCG 
ACAGCGCCAC AGTCGTCGAC AGCCACTGAC AAGAAAACCA GAAAGCGACG AAAAGCTGGT 
CCAACTGAAG ATCAGGCAAC TCCGAAACAA GAAAGGAAAA TCACAGAATT CATGAAAGTT 
GGAGGAGAGG TTGCAAGTGG AAATAGTGTT GCACGATGTC TATTGACGGA ATATCATCAA 
AATCAAGGAT CACCGAAGAG GCAGCCAGCT GTACAGCAGA ATGGATCTAA CAGTTACGAT 
TCACAGCAGC AGCAACCACA AATGAATCAG CATGAAATGC AAAACTCATA TTGGGGAGTT 
GCTACACCGT CATTGGGTGT TAATAATCGT GGAACCCCGA CTCCAACTCA ACAACAACAT 
TATTCATCGG ATTCAAACTC AAACTCGAAT CAATCACCAC CTGGTCAGGG AAATCAATCA 
GGAAGGATGG TGAGAACAAT CGATGAAGAG ACTCAGACGG ATTCTTCGCT ATCTCAGGCT 
AATCCTCAAA ATGCTGATGA AGTCGCCAAA ATGAATAGAA TCATTGAAGA CCATCGTCGC 
CAAATCGAAG AGTTGAACTC GAAGAATAGT TTGGAACGAA GAAAGAATGA AGCTTCGAAA 
GAAACAATCA AGCGATTACT TATCGATAAG AATCAGATCG AACGAAAAGC CCTTCGCGAC 
AAAACGGCTG CCGATTCACC TCGAATTGGA TGTTTCAAAA CTACTCGAAC TGGTGATTCA 
TTCCGTGATC AATGGGTTGA TGGGTGGGCG TTTGCCGAAA TGGATAAGAA AACTGAACAG 
ATCAATGCTG AAAGAAATGA AATTGCAAGT GCTTCTGCCT TGTTGAAAAA ACGAAAGCCT 
CTTGGAATCG GTAAAGAACC TAAACGCCCA CAGGCAGTCA ATTCTCAAAA TGACTCCAAC 
GGTATGCAGC CATCAACAAG TTCCAATACG AATGGAGACG ATGCGATCTT CAGACGACCT 
GAAGAACCGA AAGAAATCCA GTACCAAGAG TACATTGAGC TCGATGAAAT CTATAAACTG 
CGCAGGGAGC ACTTGAGAAA AGAAGAAACT GATCTCTCCA TGGAGAAAGA AAGATTGGAA 
AAGGAGAAGC AGCATCATGT GAGAGAGCTG AAACGAGCTT CGAATGAAAG TGCGTCACAG 
TTCAATGATC ATCGACTCTT GCATAAAAGA TACCTCATGC TGAACTTGCT CGGTAAGGGT 
GGATTCAGTG AGGTCTGGAA GGCGTTCGAC ATTGAAGAGA ACCGGTATGT CGCATGCAAG 
ATCCATCATG TTAACAAAGA CTGGAAGGAG GAGAAGAAGG CGAACTACGT CAAGCACGCG 
ATGCGTGAAA AAGACATCCA CAAGTCATTG GATCATTGCC GAATTGTGAA GCAATACGAT 
CTGCTCACAA TCGACAATCA CTCGTTCTGC ACGGTTCTCG AATATGTGCC GGGCAACGAC 
CTCGATTTCT ACCTCAAGCA GAACAGGTCG ATCAGTGAGA AGGAGGCTCG AAGCATTATT 
ATGCAAGTTG TTTCTGCTCT TGTCTATCTG AATGAAAAGA GCACTCCCAT CATTCATTAT 
GATCTAAAGC CTGCGAATAT TCTTCTCGAG TCAGGAAACA CCAGCGGTGC AATCAAGATC 
ACCGACTTTG GGCTGTCGAA AATCATGGAG GGAGAGAGTG ACGATCATGA CTTGGGAATC 
GAGCTCACCT CACAGTTCGC TGGAACTTAT TGGTATCTTC CACCTGAGAC CTTCATCGTT 
CCACCACCAA AGATCACTTG TAAAGTCGAT GTCTGGAGTA TTGGAGTCAT CTTCTATCAA 
TGTATCTATG GAAAGAAGCC GTTCGGAAAT GATCTGACTC AACAAAAGAT CCTCGAATAC 
AATACGATTA TTAATGCAAG AGAAGTGTCA TTCCCAAGCA AGCCTCAGGT GAGCTCCGCT 
GCTCAAGATT TCATAAGAAG ATGCCTGCAG TACAGGAAAG AAGACAGAGC AGATGTTTTC 
GAGCTGGCGA AGCATGAGCT TTTCAGACCA AGAGGAGCGA TAAGAGCCTC CGTTGCCGGA 
TCCGTAAGTT CTCCAAGCAT TCCACGCAGT CCATCAGTGA ATCGTGAAGA TGACAATATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tlk-1 ( NM_181913.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_871642.2 Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), partial mRNA.

Price & Availability↑

CloneID	OCa340105
Clone ID Related Accession (Same CDS sequence)	NM_181913.3 , NM_181913.4
Accession Version	NM_181913.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase tousled-like 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_181913.2. COMPLETENESS: incomplete on both ends.

ATGTCGATGT TGTCAATGGA CGGTATCGTC GCAGGAGGCG GAAGCAGCAG TGGTGGCGGA 
GAACGAAGTT TCGTATTAGA GCAAAAGATG TTCAATACGG GCCCACAGAA CAAAGCTTTA 
CCAACGGTCC AGAGCAGCGG TTCATCATCG AATCACGCTC CGATCGTCGG CGAGTCTCCA 
CTCGGCACTG TGAGCAGCAC AATGGCTACC GGTGATACGG GTCGTGTTGG AAACGTGACC 
TATATGAGTT CCGGAATGCT CGGTGCAACA CAGTTTATGC CACAAAATTC AAGTCATCCA 
TCAACGTCTG TAATGATGCA ACAGGTACCA CCACAAAACG GTGGTGCAAC ACGTTCATCA 
CCAACTGAGA TGCAACAGTG TATGCAGGCA ATGTCAGAAG ACTCGATAGA AATGCGGGAT 
TATAATTCTG GAGTTCATCA TATGCATCCT CATCAAATGC AAATGCAACA GCAGCAGCAA 
CATCATCAGC AGCAATACAA TATGTCGTAT CATAATCATC AACAACAAAT GCAGCAGATG 
CATTACCACC AGCAGCAACA GCAATATCAG CAGCAGCAGG CTCAACATCA TCAGATGTAT 
GCTCCACAAA TTCAGCAACA GCAACAGCAG CCCCAGCAAC AGTCACAACA ACAGTCTGCC 
CAACAGCCCC AACAGTCATC GGCTGCGTTA CAACATGTGA ATGAGTCGAG CAATCTCTCG 
TCGGCTGGTA GTATTTCGGA TCGTGAACCA GAGCAACACG GTGGTACCCC TCAACGACCG 
ACAGCGCCAC AGTCGTCGAC AGCCACTGAC AAGAAAACCA GAAAGCGACG AAAAGCTGGT 
CCAACTGAAG ATCAGGCAAC TCCGAAACAA GAAAGGAAAA TCACAGAATT CATGAAAGTT 
GGAGGAGAGG TTGCAAGTGG AAATAGTGTT GCACGATGTC TATTGACGGA ATATCATCAA 
AATCAAGGAT CACCGAAGAG GCAGCCAGCT GTACAGCAGA ATGGATCTAA CAGTTACGAT 
TCACAGCAGC AGCAACCACA AATGAATCAG CATGAAATGC AAAACTCATA TTGGGGAGTT 
GCTACACCGT CATTGGGTGT TAATAATCGT GGAACCCCGA CTCCAACTCA ACAACAACAT 
TATTCATCGG ATTCAAACTC AAACTCGAAT CAATCACCAC CTGGTCAGGG AAATCAATCA 
GGAAGGATGG TGAGAACAAT CGATGAAGAG ACTCAGACGG ATTCTTCGCT ATCTCAGGCT 
AATCCTCAAA ATGCTGATGA AGTCGCCAAA ATGAATAGAA TCATTGAAGA CCATCGTCGC 
CAAATCGAAG AGTTGAACTC GAAGAATAGT TTGGAACGAA GAAAGAATGA AGCTTCGAAA 
GAAACAATCA AGCGATTACT TATCGATAAG AATCAGATCG AACGAAAAGC CCTTCGCGAC 
AAAACGGCTG CCGATTCACC TCGAATTGGA TGTTTCAAAA CTACTCGAAC TGGTGATTCA 
TTCCGTGATC AATGGGTTGA TGGGTGGGCG TTTGCCGAAA TGGATAAGAA AACTGAACAG 
ATCAATGCTG AAAGAAATGA AATTGCAAGT GCTTCTGCCT TGTTGAAAAA ACGAAAGCCT 
CTTGGAATCG GTAAAGAACC TAAACGCCCA CAGGCAGTCA ATTCTCAAAA TGACTCCAAC 
GGTATGCAGC CATCAACAAG TTCCAATACG AATGGAGACG ATGCGATCTT CAGACGACCT 
GAAGAACCGA AAGAAATCCA GTACCAAGAG TACATTGAGC TCGATGAAAT CTATAAACTG 
CGCAGGGAGC ACTTGAGAAA AGAAGAAACT GATCTCTCCA TGGAGAAAGA AAGATTGGAA 
AAGGAGAAGC AGCATCATGT GAGAGAGCTG AAACGAGCTT CGAATGAAAG TGCGTCACAG 
TTCAATGATC ATCGACTCTT GCATAAAAGA TACCTCATGC TGAACTTGCT CGGTAAGGGT 
GGATTCAGTG AGGTCTGGAA GGCGTTCGAC ATTGAAGAGA ACCGGTATGT CGCATGCAAG 
ATCCATCATG TTAACAAAGA CTGGAAGGAG GAGAAGAAGG CGAACTACGT CAAGCACGCG 
ATGCGTGAAA AAGACATCCA CAAGTCATTG GATCATTGCC GAATTGTGAA GCAATACGAT 
CTGCTCACAA TCGACAATCA CTCGTTCTGC ACGGTTCTCG AATATGTGCC GGGCAACGAC 
CTCGATTTCT ACCTCAAGCA GAACAGGTCG ATCAGTGAGA AGGAGGCTCG AAGCATTATT 
ATGCAAGTTG TTTCTGCTCT TGTCTATCTG AATGAAAAGA GCACTCCCAT CATTCATTAT 
GATCTAAAGC CTGCGAATAT TCTTCTCGAG TCAGGAAACA CCAGCGGTGC AATCAAGATC 
ACCGACTTTG GGCTGTCGAA AATCATGGAG GGAGAGAGTG ACGATCATGA CTTGGGAATC 
GAGCTCACCT CACAGTTCGC TGGAACTTAT TGGTATCTTC CACCTGAGAC CTTCATCGTT 
CCACCACCAA AGATCACTTG TAAAGTCGAT GTCTGGAGTA TTGGAGTCAT CTTCTATCAA 
TGTATCTATG GAAAGAAGCC GTTCGGAAAT GATCTGACTC AACAAAAGAT CCTCGAATAC 
AATACGATTA TTAATGCAAG AGAAGTGTCA TTCCCAAGCA AGCCTCAGGT GAGCTCCGCT 
GCTCAAGATT TCATAAGAAG ATGCCTGCAG TACAGGAAAG AAGACAGAGC AGATGTTTTC 
GAGCTGGCGA AGCATGAGCT TTTCAGACCA AGAGGAGCGA TAAGAGCCTC CGTTGCCGGA 
TCCCTCTTAC TTGTAATAAT CACGTTGTGT CAACATTCGA ACAGATCCGA AGACCGTTTG 
TGTGTGAGCA ACGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_871642.2
CDS	1..2898
Translation	MSMLSMDGIVAGGGSSSGGGERSFVLEQKMFNTGPQNKALPTVQSSGSSSNHAPIVGESPLGTVSSTMATGDTGRVGNVTYMSSGMLGATQFMPQNSSHPSTSVMMQQVPPQNGGATRSSPTEMQQCMQAMSEDSIEMRDYNSGVHHMHPHQMQMQQQQQHHQQQYNMSYHNHQQQMQQMHYHQQQQQYQQQQAQHHQMYAPQIQQQQQQPQQQSQQQSAQQPQQSSAALQHVNESSNLSSAGSISDREPEQHGGTPQRPTAPQSSTATDKKTRKRRKAGPTEDQATPKQERKITEFMKVGGEVASGNSVARCLLTEYHQNQGSPKRQPAVQQNGSNSYDSQQQQPQMNQHEMQNSYWGVATPSLGVNNRGTPTPTQQQHYSSDSNSNSNQSPPGQGNQSGRMVRTIDEETQTDSSLSQANPQNADEVAKMNRIIEDHRRQIEELNSKNSLERRKNEASKETIKRLLIDKNQIERKALRDKTAADSPRIGCFKTTRTGDSFRDQWVDGWAFAEMDKKTEQINAERNEIASASALLKKRKPLGIGKEPKRPQAVNSQNDSNGMQPSTSSNTNGDDAIFRRPEEPKEIQYQEYIELDEIYKLRREHLRKEETDLSMEKERLEKEKQHHVRELKRASNESASQFNDHRLLHKRYLMLNLLGKGGFSEVWKAFDIEENRYVACKIHHVNKDWKEEKKANYVKHAMREKDIHKSLDHCRIVKQYDLLTIDNHSFCTVLEYVPGNDLDFYLKQNRSISEKEARSIIMQVVSALVYLNEKSTPIIHYDLKPANILLESGNTSGAIKITDFGLSKIMEGESDDHDLGIELTSQFAGTYWYLPPETFIVPPPKITCKVDVWSIGVIFYQCIYGKKPFGNDLTQQKILEYNTIINAREVSFPSKPQVSSAAQDFIRRCLQYRKEDRADVFELAKHELFRPRGAIRASVAGSLLLVIITLCQHSNRSEDRLCVSNV

Target ORF information:

RefSeq Version	NM_181913.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181913.3

ORF Insert Sequence:

ATGTCGATGT TGTCAATGGA CGGTATCGTC GCAGGAGGCG GAAGCAGCAG TGGTGGCGGA 
GAACGAAGTT TCGTATTAGA GCAAAAGATG TTCAATACGG GCCCACAGAA CAAAGCTTTA 
CCAACGGTCC AGAGCAGCGG TTCATCATCG AATCACGCTC CGATCGTCGG CGAGTCTCCA 
CTCGGCACTG TGAGCAGCAC AATGGCTACC GGTGATACGG GTCGTGTTGG AAACGTGACC 
TATATGAGTT CCGGAATGCT CGGTGCAACA CAGTTTATGC CACAAAATTC AAGTCATCCA 
TCAACGTCTG TAATGATGCA ACAGGTACCA CCACAAAACG GTGGTGCAAC ACGTTCATCA 
CCAACTGAGA TGCAACAGTG TATGCAGGCA ATGTCAGAAG ACTCGATAGA AATGCGGGAT 
TATAATTCTG GAGTTCATCA TATGCATCCT CATCAAATGC AAATGCAACA GCAGCAGCAA 
CATCATCAGC AGCAATACAA TATGTCGTAT CATAATCATC AACAACAAAT GCAGCAGATG 
CATTACCACC AGCAGCAACA GCAATATCAG CAGCAGCAGG CTCAACATCA TCAGATGTAT 
GCTCCACAAA TTCAGCAACA GCAACAGCAG CCCCAGCAAC AGTCACAACA ACAGTCTGCC 
CAACAGCCCC AACAGTCATC GGCTGCGTTA CAACATGTGA ATGAGTCGAG CAATCTCTCG 
TCGGCTGGTA GTATTTCGGA TCGTGAACCA GAGCAACACG GTGGTACCCC TCAACGACCG 
ACAGCGCCAC AGTCGTCGAC AGCCACTGAC AAGAAAACCA GAAAGCGACG AAAAGCTGGT 
CCAACTGAAG ATCAGGCAAC TCCGAAACAA GAAAGGAAAA TCACAGAATT CATGAAAGTT 
GGAGGAGAGG TTGCAAGTGG AAATAGTGTT GCACGATGTC TATTGACGGA ATATCATCAA 
AATCAAGGAT CACCGAAGAG GCAGCCAGCT GTACAGCAGA ATGGATCTAA CAGTTACGAT 
TCACAGCAGC AGCAACCACA AATGAATCAG CATGAAATGC AAAACTCATA TTGGGGAGTT 
GCTACACCGT CATTGGGTGT TAATAATCGT GGAACCCCGA CTCCAACTCA ACAACAACAT 
TATTCATCGG ATTCAAACTC AAACTCGAAT CAATCACCAC CTGGTCAGGG AAATCAATCA 
GGAAGGATGG TGAGAACAAT CGATGAAGAG ACTCAGACGG ATTCTTCGCT ATCTCAGGCT 
AATCCTCAAA ATGCTGATGA AGTCGCCAAA ATGAATAGAA TCATTGAAGA CCATCGTCGC 
CAAATCGAAG AGTTGAACTC GAAGAATAGT TTGGAACGAA GAAAGAATGA AGCTTCGAAA 
GAAACAATCA AGCGATTACT TATCGATAAG AATCAGATCG AACGAAAAGC CCTTCGCGAC 
AAAACGGCTG CCGATTCACC TCGAATTGGA TGTTTCAAAA CTACTCGAAC TGGTGATTCA 
TTCCGTGATC AATGGGTTGA TGGGTGGGCG TTTGCCGAAA TGGATAAGAA AACTGAACAG 
ATCAATGCTG AAAGAAATGA AATTGCAAGT GCTTCTGCCT TGTTGAAAAA ACGAAAGCCT 
CTTGGAATCG GTAAAGAACC TAAACGCCCA CAGGCAGTCA ATTCTCAAAA TGACTCCAAC 
GGTATGCAGC CATCAACAAG TTCCAATACG AATGGAGACG ATGCGATCTT CAGACGACCT 
GAAGAACCGA AAGAAATCCA GTACCAAGAG TACATTGAGC TCGATGAAAT CTATAAACTG 
CGCAGGGAGC ACTTGAGAAA AGAAGAAACT GATCTCTCCA TGGAGAAAGA AAGATTGGAA 
AAGGAGAAGC AGCATCATGT GAGAGAGCTG AAACGAGCTT CGAATGAAAG TGCGTCACAG 
TTCAATGATC ATCGACTCTT GCATAAAAGA TACCTCATGC TGAACTTGCT CGGTAAGGGT 
GGATTCAGTG AGGTCTGGAA GGCGTTCGAC ATTGAAGAGA ACCGGTATGT CGCATGCAAG 
ATCCATCATG TTAACAAAGA CTGGAAGGAG GAGAAGAAGG CGAACTACGT CAAGCACGCG 
ATGCGTGAAA AAGACATCCA CAAGTCATTG GATCATTGCC GAATTGTGAA GCAATACGAT 
CTGCTCACAA TCGACAATCA CTCGTTCTGC ACGGTTCTCG AATATGTGCC GGGCAACGAC 
CTCGATTTCT ACCTCAAGCA GAACAGGTCG ATCAGTGAGA AGGAGGCTCG AAGCATTATT 
ATGCAAGTTG TTTCTGCTCT TGTCTATCTG AATGAAAAGA GCACTCCCAT CATTCATTAT 
GATCTAAAGC CTGCGAATAT TCTTCTCGAG TCAGGAAACA CCAGCGGTGC AATCAAGATC 
ACCGACTTTG GGCTGTCGAA AATCATGGAG GGAGAGAGTG ACGATCATGA CTTGGGAATC 
GAGCTCACCT CACAGTTCGC TGGAACTTAT TGGTATCTTC CACCTGAGAC CTTCATCGTT 
CCACCACCAA AGATCACTTG TAAAGTCGAT GTCTGGAGTA TTGGAGTCAT CTTCTATCAA 
TGTATCTATG GAAAGAAGCC GTTCGGAAAT GATCTGACTC AACAAAAGAT CCTCGAATAC 
AATACGATTA TTAATGCAAG AGAAGTGTCA TTCCCAAGCA AGCCTCAGGT GAGCTCCGCT 
GCTCAAGATT TCATAAGAAG ATGCCTGCAG TACAGGAAAG AAGACAGAGC AGATGTTTTC 
GAGCTGGCGA AGCATGAGCT TTTCAGACCA AGAGGAGCGA TAAGAGCCTC CGTTGCCGGA 
TCCCTCTTAC TTGTAATAAT CACGTTGTGT CAACATTCGA ACAGATCCGA AGACCGTTTG 
TGTGTGAGCA ACGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tlk-1 ( NM_001268088.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255017.1 Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), partial mRNA.

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CloneID	OCa340531
Clone ID Related Accession (Same CDS sequence)	NM_001268088.2 , NM_001268088.1
Accession Version	NM_001268088.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase tousled-like 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGAGTTCCG GAATGCTCGG TGCAACACAG TTTATGCCAC AAAATTCAAG TCATCCATCA 
ACGTCTGTAA TGATGCAACA GGTACCACCA CAAAACGGTG GTGCAACACG TTCATCACCA 
ACTGAGATGC AACAGTGTAT GCAGGCAATG TCAGAAGACT CGATAGAAAT GCGGGATTAT 
AATTCTGGAG TTCATCATAT GCATCCTCAT CAAATGCAAA TGCAACAGCA GCAGCAACAT 
CATCAGCAGC AATACAATAT GTCGTATCAT AATCATCAAC AACAAATGCA GCAGATGCAT 
TACCACCAGC AGCAACAGCA ATATCAGCAG CAGCAGGCTC AACATCATCA GATGTATGCT 
CCACAAATTC AGCAACAGCA ACAGCAGCCC CAGCAACAGT CACAACAACA GTCTGCCCAA 
CAGCCCCAAC AGTCATCGGC TGCGTTACAA CATGTGAATG AGTCGAGCAA TCTCTCGTCG 
GCTGGTAGTA TTTCGGATCG TGAACCAGAG CAACACGGTG GTACCCCTCA ACGACCGACA 
GCGCCACAGT CGTCGACAGC CACTGACAAG AAAACCAGAA AGCGACGAAA AGCTGGTCCA 
ACTGAAGATC AGGCAACTCC GAAACAAGAA AGGAAAATCA CAGAATTCAT GAAAGTTGGA 
GGAGAGGTTG CAAGTGGAAA TAGTGTTGCA CGATGTCTAT TGACGGAATA TCATCAAAAT 
CAAGGATCAC CGAAGAGGCA GCCAGCTGTA CAGCAGAATG GATCTAACAG TTACGATTCA 
CAGCAGCAGC AACCACAAAT GAATCAGCAT GAAATGCAAA ACTCATATTG GGGAGTTGCT 
ACACCGTCAT TGGGTGTTAA TAATCGTGGA ACCCCGACTC CAACTCAACA ACAACATTAT 
TCATCGGATT CAAACTCAAA CTCGAATCAA TCACCACCTG GTCAGGGAAA TCAATCAGGA 
AGGATGGTGA GAACAATCGA TGAAGAGACT CAGACGGATT CTTCGCTATC TCAGGCTAAT 
CCTCAAAATG CTGATGAAGT CGCCAAAATG AATAGAATCA TTGAAGACCA TCGTCGCCAA 
ATCGAAGAGT TGAACTCGAA GAATAGTTTG GAACGAAGAA AGAATGAAGC TTCGAAAGAA 
ACAATCAAGC GATTACTTAT CGATAAGAAT CAGATCGAAC GAAAAGCCCT TCGCGACAAA 
ACGGCTGCCG ATTCACCTCG AATTGGATGT TTCAAAACTA CTCGAACTGG TGATTCATTC 
CGTGATCAAT GGGTTGATGG GTGGGCGTTT GCCGAAATGG ATAAGAAAAC TGAACAGATC 
AATGCTGAAA GAAATGAAAT TGCAAGTGCT TCTGCCTTGT TGAAAAAACG AAAGCCTCTT 
GGAATCGGTA AAGAACCTAA ACGCCCACAG GCAGTCAATT CTCAAAATGA CTCCAACGGT 
ATGCAGCCAT CAACAAGTTC CAATACGAAT GGAGACGATG CGATCTTCAG ACGACCTGAA 
GAACCGAAAG AAATCCAGTA CCAAGAGTAC ATTGAGCTCG ATGAAATCTA TAAACTGCGC 
AGGGAGCACT TGAGAAAAGA AGAAACTGAT CTCTCCATGG AGAAAGAAAG ATTGGAAAAG 
GAGAAGCAGC ATCATGTGAG AGAGCTGAAA CGAGCTTCGA ATGAAAGTGC GTCACAGTTC 
AATGATCATC GACTCTTGCA TAAAAGATAC CTCATGCTGA ACTTGCTCGG TAAGGGTGGA 
TTCAGTGAGG TCTGGAAGGC GTTCGACATT GAAGAGAACC GGTATGTCGC ATGCAAGATC 
CATCATGTTA ACAAAGACTG GAAGGAGGAG AAGAAGGCGA ACTACGTCAA GCACGCGATG 
CGTGAAAAAG ACATCCACAA GTCATTGGAT CATTGCCGAA TTGTGAAGCA ATACGATCTG 
CTCACAATCG ACAATCACTC GTTCTGCACG GTTCTCGAAT ATGTGCCGGG CAACGACCTC 
GATTTCTACC TCAAGCAGAA CAGGTCGATC AGTGAGAAGG AGGCTCGAAG CATTATTATG 
CAAGTTGTTT CTGCTCTTGT CTATCTGAAT GAAAAGAGCA CTCCCATCAT TCATTATGAT 
CTAAAGCCTG CGAATATTCT TCTCGAGTCA GGAAACACCA GCGGTGCAAT CAAGATCACC 
GACTTTGGGC TGTCGAAAAT CATGGAGGGA GAGAGTGACG ATCATGACTT GGGAATCGAG 
CTCACCTCAC AGTTCGCTGG AACTTATTGG TATCTTCCAC CTGAGACCTT CATCGTTCCA 
CCACCAAAGA TCACTTGTAA AGTCGATGTC TGGAGTATTG GAGTCATCTT CTATCAATGT 
ATCTATGGAA AGAAGCCGTT CGGAAATGAT CTGACTCAAC AAAAGATCCT CGAATACAAT 
ACGATTATTA ATGCAAGAGA AGTGTCATTC CCAAGCAAGC CTCAGGTGAG CTCCGCTGCT 
CAAGATTTCA TAAGAAGATG CCTGCAGTAC AGGAAAGAAG ACAGAGCAGA TGTTTTCGAG 
CTGGCGAAGC ATGAGCTTTT CAGACCAAGA GGAGCGATAA GAGCCTCCGT TGCCGGATCC 
CTCTTACTTG TAATAATCAC GTTGTGTCAA CATTCGAACA GATCCGAAGA CCGTTTGTGT 
GTGAGCAACG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255017.1
CDS	1..2655
Translation	MSSGMLGATQFMPQNSSHPSTSVMMQQVPPQNGGATRSSPTEMQQCMQAMSEDSIEMRDYNSGVHHMHPHQMQMQQQQQHHQQQYNMSYHNHQQQMQQMHYHQQQQQYQQQQAQHHQMYAPQIQQQQQQPQQQSQQQSAQQPQQSSAALQHVNESSNLSSAGSISDREPEQHGGTPQRPTAPQSSTATDKKTRKRRKAGPTEDQATPKQERKITEFMKVGGEVASGNSVARCLLTEYHQNQGSPKRQPAVQQNGSNSYDSQQQQPQMNQHEMQNSYWGVATPSLGVNNRGTPTPTQQQHYSSDSNSNSNQSPPGQGNQSGRMVRTIDEETQTDSSLSQANPQNADEVAKMNRIIEDHRRQIEELNSKNSLERRKNEASKETIKRLLIDKNQIERKALRDKTAADSPRIGCFKTTRTGDSFRDQWVDGWAFAEMDKKTEQINAERNEIASASALLKKRKPLGIGKEPKRPQAVNSQNDSNGMQPSTSSNTNGDDAIFRRPEEPKEIQYQEYIELDEIYKLRREHLRKEETDLSMEKERLEKEKQHHVRELKRASNESASQFNDHRLLHKRYLMLNLLGKGGFSEVWKAFDIEENRYVACKIHHVNKDWKEEKKANYVKHAMREKDIHKSLDHCRIVKQYDLLTIDNHSFCTVLEYVPGNDLDFYLKQNRSISEKEARSIIMQVVSALVYLNEKSTPIIHYDLKPANILLESGNTSGAIKITDFGLSKIMEGESDDHDLGIELTSQFAGTYWYLPPETFIVPPPKITCKVDVWSIGVIFYQCIYGKKPFGNDLTQQKILEYNTIINAREVSFPSKPQVSSAAQDFIRRCLQYRKEDRADVFELAKHELFRPRGAIRASVAGSLLLVIITLCQHSNRSEDRLCVSNV

Target ORF information:

RefSeq Version	NM_001268088.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268088.1

ORF Insert Sequence:

ATGAGTTCCG GAATGCTCGG TGCAACACAG TTTATGCCAC AAAATTCAAG TCATCCATCA 
ACGTCTGTAA TGATGCAACA GGTACCACCA CAAAACGGTG GTGCAACACG TTCATCACCA 
ACTGAGATGC AACAGTGTAT GCAGGCAATG TCAGAAGACT CGATAGAAAT GCGGGATTAT 
AATTCTGGAG TTCATCATAT GCATCCTCAT CAAATGCAAA TGCAACAGCA GCAGCAACAT 
CATCAGCAGC AATACAATAT GTCGTATCAT AATCATCAAC AACAAATGCA GCAGATGCAT 
TACCACCAGC AGCAACAGCA ATATCAGCAG CAGCAGGCTC AACATCATCA GATGTATGCT 
CCACAAATTC AGCAACAGCA ACAGCAGCCC CAGCAACAGT CACAACAACA GTCTGCCCAA 
CAGCCCCAAC AGTCATCGGC TGCGTTACAA CATGTGAATG AGTCGAGCAA TCTCTCGTCG 
GCTGGTAGTA TTTCGGATCG TGAACCAGAG CAACACGGTG GTACCCCTCA ACGACCGACA 
GCGCCACAGT CGTCGACAGC CACTGACAAG AAAACCAGAA AGCGACGAAA AGCTGGTCCA 
ACTGAAGATC AGGCAACTCC GAAACAAGAA AGGAAAATCA CAGAATTCAT GAAAGTTGGA 
GGAGAGGTTG CAAGTGGAAA TAGTGTTGCA CGATGTCTAT TGACGGAATA TCATCAAAAT 
CAAGGATCAC CGAAGAGGCA GCCAGCTGTA CAGCAGAATG GATCTAACAG TTACGATTCA 
CAGCAGCAGC AACCACAAAT GAATCAGCAT GAAATGCAAA ACTCATATTG GGGAGTTGCT 
ACACCGTCAT TGGGTGTTAA TAATCGTGGA ACCCCGACTC CAACTCAACA ACAACATTAT 
TCATCGGATT CAAACTCAAA CTCGAATCAA TCACCACCTG GTCAGGGAAA TCAATCAGGA 
AGGATGGTGA GAACAATCGA TGAAGAGACT CAGACGGATT CTTCGCTATC TCAGGCTAAT 
CCTCAAAATG CTGATGAAGT CGCCAAAATG AATAGAATCA TTGAAGACCA TCGTCGCCAA 
ATCGAAGAGT TGAACTCGAA GAATAGTTTG GAACGAAGAA AGAATGAAGC TTCGAAAGAA 
ACAATCAAGC GATTACTTAT CGATAAGAAT CAGATCGAAC GAAAAGCCCT TCGCGACAAA 
ACGGCTGCCG ATTCACCTCG AATTGGATGT TTCAAAACTA CTCGAACTGG TGATTCATTC 
CGTGATCAAT GGGTTGATGG GTGGGCGTTT GCCGAAATGG ATAAGAAAAC TGAACAGATC 
AATGCTGAAA GAAATGAAAT TGCAAGTGCT TCTGCCTTGT TGAAAAAACG AAAGCCTCTT 
GGAATCGGTA AAGAACCTAA ACGCCCACAG GCAGTCAATT CTCAAAATGA CTCCAACGGT 
ATGCAGCCAT CAACAAGTTC CAATACGAAT GGAGACGATG CGATCTTCAG ACGACCTGAA 
GAACCGAAAG AAATCCAGTA CCAAGAGTAC ATTGAGCTCG ATGAAATCTA TAAACTGCGC 
AGGGAGCACT TGAGAAAAGA AGAAACTGAT CTCTCCATGG AGAAAGAAAG ATTGGAAAAG 
GAGAAGCAGC ATCATGTGAG AGAGCTGAAA CGAGCTTCGA ATGAAAGTGC GTCACAGTTC 
AATGATCATC GACTCTTGCA TAAAAGATAC CTCATGCTGA ACTTGCTCGG TAAGGGTGGA 
TTCAGTGAGG TCTGGAAGGC GTTCGACATT GAAGAGAACC GGTATGTCGC ATGCAAGATC 
CATCATGTTA ACAAAGACTG GAAGGAGGAG AAGAAGGCGA ACTACGTCAA GCACGCGATG 
CGTGAAAAAG ACATCCACAA GTCATTGGAT CATTGCCGAA TTGTGAAGCA ATACGATCTG 
CTCACAATCG ACAATCACTC GTTCTGCACG GTTCTCGAAT ATGTGCCGGG CAACGACCTC 
GATTTCTACC TCAAGCAGAA CAGGTCGATC AGTGAGAAGG AGGCTCGAAG CATTATTATG 
CAAGTTGTTT CTGCTCTTGT CTATCTGAAT GAAAAGAGCA CTCCCATCAT TCATTATGAT 
CTAAAGCCTG CGAATATTCT TCTCGAGTCA GGAAACACCA GCGGTGCAAT CAAGATCACC 
GACTTTGGGC TGTCGAAAAT CATGGAGGGA GAGAGTGACG ATCATGACTT GGGAATCGAG 
CTCACCTCAC AGTTCGCTGG AACTTATTGG TATCTTCCAC CTGAGACCTT CATCGTTCCA 
CCACCAAAGA TCACTTGTAA AGTCGATGTC TGGAGTATTG GAGTCATCTT CTATCAATGT 
ATCTATGGAA AGAAGCCGTT CGGAAATGAT CTGACTCAAC AAAAGATCCT CGAATACAAT 
ACGATTATTA ATGCAAGAGA AGTGTCATTC CCAAGCAAGC CTCAGGTGAG CTCCGCTGCT 
CAAGATTTCA TAAGAAGATG CCTGCAGTAC AGGAAAGAAG ACAGAGCAGA TGTTTTCGAG 
CTGGCGAAGC ATGAGCTTTT CAGACCAAGA GGAGCGATAA GAGCCTCCGT TGCCGGATCC 
CTCTTACTTG TAATAATCAC GTTGTGTCAA CATTCGAACA GATCCGAAGA CCGTTTGTGT 
GTGAGCAACG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tlk-1 ( NM_001047379.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001040844.1 Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), partial mRNA.

Price & Availability↑

CloneID	OCa340577
Clone ID Related Accession (Same CDS sequence)	NM_001047379.1 , NM_001047379.2
Accession Version	NM_001047379.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase tousled-like 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGGCTACCG GTGATACGGG TCGTGTTGGA AACGTGACCT ATATGAGTTC CGGAATGCTC 
GGTGCAACAC AGTTTATGCC ACAAAATTCA AGTCATCCAT CAACGTCTGT AATGATGCAA 
CAGGTACCAC CACAAAACGG TGGTGCAACA CGTTCATCAC CAACTGAGAT GCAACAGTGT 
ATGCAGGCAA TGTCAGAAGA CTCGATAGAA ATGCGGGATT ATAATTCTGG AGTTCATCAT 
ATGCATCCTC ATCAAATGCA AATGCAACAG CAGCAGCAAC ATCATCAGCA GCAATACAAT 
ATGTCGTATC ATAATCATCA ACAACAAATG CAGCAGATGC ATTACCACCA GCAGCAACAG 
CAATATCAGC AGCAGCAGGC TCAACATCAT CAGATGTATG CTCCACAAAT TCAGCAACAG 
CAACAGCAGC CCCAGCAACA GTCACAACAA CAGTCTGCCC AACAGCCCCA ACAGTCATCG 
GCTGCGTTAC AACATGTGAA TGAGTCGAGC AATCTCTCGT CGGCTGGTAG TATTTCGGAT 
CGTGAACCAG AGCAACACGG TGGTACCCCT CAACGACCGA CAGCGCCACA GTCGTCGACA 
GCCACTGACA AGAAAACCAG AAAGCGACGA AAAGCTGGTC CAACTGAAGA TCAGGCAACT 
CCGAAACAAG AAAGGAAAAT CACAGAATTC ATGAAAGTTG GAGGAGAGGT TGCAAGTGGA 
AATAGTGTTG CACGATGTCT ATTGACGGAA TATCATCAAA ATCAAGGATC ACCGAAGAGG 
CAGCCAGCTG TACAGCAGAA TGGATCTAAC AGTTACGATT CACAGCAGCA GCAACCACAA 
ATGAATCAGC ATGAAATGCA AAACTCATAT TGGGGAGTTG CTACACCGTC ATTGGGTGTT 
AATAATCGTG GAACCCCGAC TCCAACTCAA CAACAACATT ATTCATCGGA TTCAAACTCA 
AACTCGAATC AATCACCACC TGGTCAGGGA AATCAATCAG GAAGGATGGT GAGAACAATC 
GATGAAGAGA CTCAGACGGA TTCTTCGCTA TCTCAGGCTA ATCCTCAAAA TGCTGATGAA 
GTCGCCAAAA TGAATAGAAT CATTGAAGAC CATCGTCGCC AAATCGAAGA GTTGAACTCG 
AAGAATAGTT TGGAACGAAG AAAGAATGAA GCTTCGAAAG AAACAATCAA GCGATTACTT 
ATCGATAAGA ATCAGATCGA ACGAAAAGCC CTTCGCGACA AAACGGCTGC CGATTCACCT 
CGAATTGGAT GTTTCAAAAC TACTCGAACT GGTGATTCAT TCCGTGATCA ATGGGTTGAT 
GGGTGGGCGT TTGCCGAAAT GGATAAGAAA ACTGAACAGA TCAATGCTGA AAGAAATGAA 
ATTGCAAGTG CTTCTGCCTT GTTGAAAAAA CGAAAGCCTC TTGGAATCGG TAAAGAACCT 
AAACGCCCAC AGGCAGTCAA TTCTCAAAAT GACTCCAACG GTATGCAGCC ATCAACAAGT 
TCCAATACGA ATGGAGACGA TGCGATCTTC AGACGACCTG AAGAACCGAA AGAAATCCAG 
TACCAAGAGT ACATTGAGCT CGATGAAATC TATAAACTGC GCAGGGAGCA CTTGAGAAAA 
GAAGAAACTG ATCTCTCCAT GGAGAAAGAA AGATTGGAAA AGGAGAAGCA GCATCATGTG 
AGAGAGCTGA AACGAGCTTC GAATGAAAGT GCGTCACAGT TCAATGATCA TCGACTCTTG 
CATAAAAGAT ACCTCATGCT GAACTTGCTC GGTAAGGGTG GATTCAGTGA GGTCTGGAAG 
GCGTTCGACA TTGAAGAGAA CCGGTATGTC GCATGCAAGA TCCATCATGT TAACAAAGAC 
TGGAAGGAGG AGAAGAAGGC GAACTACGTC AAGCACGCGA TGCGTGAAAA AGACATCCAC 
AAGTCATTGG ATCATTGCCG AATTGTGAAG CAATACGATC TGCTCACAAT CGACAATCAC 
TCGTTCTGCA CGGTTCTCGA ATATGTGCCG GGCAACGACC TCGATTTCTA CCTCAAGCAG 
AACAGGTCGA TCAGTGAGAA GGAGGCTCGA AGCATTATTA TGCAAGTTGT TTCTGCTCTT 
GTCTATCTGA ATGAAAAGAG CACTCCCATC ATTCATTATG ATCTAAAGCC TGCGAATATT 
CTTCTCGAGT CAGGAAACAC CAGCGGTGCA ATCAAGATCA CCGACTTTGG GCTGTCGAAA 
ATCATGGAGG GAGAGAGTGA CGATCATGAC TTGGGAATCG AGCTCACCTC ACAGTTCGCT 
GGAACTTATT GGTATCTTCC ACCTGAGACC TTCATCGTTC CACCACCAAA GATCACTTGT 
AAAGTCGATG TCTGGAGTAT TGGAGTCATC TTCTATCAAT GTATCTATGG AAAGAAGCCG 
TTCGGAAATG ATCTGACTCA ACAAAAGATC CTCGAATACA ATACGATTAT TAATGCAAGA 
GAAGTGTCAT TCCCAAGCAA GCCTCAGGTG AGCTCCGCTG CTCAAGATTT CATAAGAAGA 
TGCCTGCAGT ACAGGAAAGA AGACAGAGCA GATGTTTTCG AGCTGGCGAA GCATGAGCTT 
TTCAGACCAA GAGGAGCGAT AAGAGCCTCC GTTGCCGGAT CCGTAAGTTC TCCAAGCATT 
CCACGCAGTC CATCAGTGAA TCGTGAAGAT GACAATATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001040844.1
CDS	1..2682
Translation	MATGDTGRVGNVTYMSSGMLGATQFMPQNSSHPSTSVMMQQVPPQNGGATRSSPTEMQQCMQAMSEDSIEMRDYNSGVHHMHPHQMQMQQQQQHHQQQYNMSYHNHQQQMQQMHYHQQQQQYQQQQAQHHQMYAPQIQQQQQQPQQQSQQQSAQQPQQSSAALQHVNESSNLSSAGSISDREPEQHGGTPQRPTAPQSSTATDKKTRKRRKAGPTEDQATPKQERKITEFMKVGGEVASGNSVARCLLTEYHQNQGSPKRQPAVQQNGSNSYDSQQQQPQMNQHEMQNSYWGVATPSLGVNNRGTPTPTQQQHYSSDSNSNSNQSPPGQGNQSGRMVRTIDEETQTDSSLSQANPQNADEVAKMNRIIEDHRRQIEELNSKNSLERRKNEASKETIKRLLIDKNQIERKALRDKTAADSPRIGCFKTTRTGDSFRDQWVDGWAFAEMDKKTEQINAERNEIASASALLKKRKPLGIGKEPKRPQAVNSQNDSNGMQPSTSSNTNGDDAIFRRPEEPKEIQYQEYIELDEIYKLRREHLRKEETDLSMEKERLEKEKQHHVRELKRASNESASQFNDHRLLHKRYLMLNLLGKGGFSEVWKAFDIEENRYVACKIHHVNKDWKEEKKANYVKHAMREKDIHKSLDHCRIVKQYDLLTIDNHSFCTVLEYVPGNDLDFYLKQNRSISEKEARSIIMQVVSALVYLNEKSTPIIHYDLKPANILLESGNTSGAIKITDFGLSKIMEGESDDHDLGIELTSQFAGTYWYLPPETFIVPPPKITCKVDVWSIGVIFYQCIYGKKPFGNDLTQQKILEYNTIINAREVSFPSKPQVSSAAQDFIRRCLQYRKEDRADVFELAKHELFRPRGAIRASVAGSVSSPSIPRSPSVNREDDNM

Target ORF information:

RefSeq Version	NM_001047379.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase tousled-like 1 (tlk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047379.1

ORF Insert Sequence:

ATGGCTACCG GTGATACGGG TCGTGTTGGA AACGTGACCT ATATGAGTTC CGGAATGCTC 
GGTGCAACAC AGTTTATGCC ACAAAATTCA AGTCATCCAT CAACGTCTGT AATGATGCAA 
CAGGTACCAC CACAAAACGG TGGTGCAACA CGTTCATCAC CAACTGAGAT GCAACAGTGT 
ATGCAGGCAA TGTCAGAAGA CTCGATAGAA ATGCGGGATT ATAATTCTGG AGTTCATCAT 
ATGCATCCTC ATCAAATGCA AATGCAACAG CAGCAGCAAC ATCATCAGCA GCAATACAAT 
ATGTCGTATC ATAATCATCA ACAACAAATG CAGCAGATGC ATTACCACCA GCAGCAACAG 
CAATATCAGC AGCAGCAGGC TCAACATCAT CAGATGTATG CTCCACAAAT TCAGCAACAG 
CAACAGCAGC CCCAGCAACA GTCACAACAA CAGTCTGCCC AACAGCCCCA ACAGTCATCG 
GCTGCGTTAC AACATGTGAA TGAGTCGAGC AATCTCTCGT CGGCTGGTAG TATTTCGGAT 
CGTGAACCAG AGCAACACGG TGGTACCCCT CAACGACCGA CAGCGCCACA GTCGTCGACA 
GCCACTGACA AGAAAACCAG AAAGCGACGA AAAGCTGGTC CAACTGAAGA TCAGGCAACT 
CCGAAACAAG AAAGGAAAAT CACAGAATTC ATGAAAGTTG GAGGAGAGGT TGCAAGTGGA 
AATAGTGTTG CACGATGTCT ATTGACGGAA TATCATCAAA ATCAAGGATC ACCGAAGAGG 
CAGCCAGCTG TACAGCAGAA TGGATCTAAC AGTTACGATT CACAGCAGCA GCAACCACAA 
ATGAATCAGC ATGAAATGCA AAACTCATAT TGGGGAGTTG CTACACCGTC ATTGGGTGTT 
AATAATCGTG GAACCCCGAC TCCAACTCAA CAACAACATT ATTCATCGGA TTCAAACTCA 
AACTCGAATC AATCACCACC TGGTCAGGGA AATCAATCAG GAAGGATGGT GAGAACAATC 
GATGAAGAGA CTCAGACGGA TTCTTCGCTA TCTCAGGCTA ATCCTCAAAA TGCTGATGAA 
GTCGCCAAAA TGAATAGAAT CATTGAAGAC CATCGTCGCC AAATCGAAGA GTTGAACTCG 
AAGAATAGTT TGGAACGAAG AAAGAATGAA GCTTCGAAAG AAACAATCAA GCGATTACTT 
ATCGATAAGA ATCAGATCGA ACGAAAAGCC CTTCGCGACA AAACGGCTGC CGATTCACCT 
CGAATTGGAT GTTTCAAAAC TACTCGAACT GGTGATTCAT TCCGTGATCA ATGGGTTGAT 
GGGTGGGCGT TTGCCGAAAT GGATAAGAAA ACTGAACAGA TCAATGCTGA AAGAAATGAA 
ATTGCAAGTG CTTCTGCCTT GTTGAAAAAA CGAAAGCCTC TTGGAATCGG TAAAGAACCT 
AAACGCCCAC AGGCAGTCAA TTCTCAAAAT GACTCCAACG GTATGCAGCC ATCAACAAGT 
TCCAATACGA ATGGAGACGA TGCGATCTTC AGACGACCTG AAGAACCGAA AGAAATCCAG 
TACCAAGAGT ACATTGAGCT CGATGAAATC TATAAACTGC GCAGGGAGCA CTTGAGAAAA 
GAAGAAACTG ATCTCTCCAT GGAGAAAGAA AGATTGGAAA AGGAGAAGCA GCATCATGTG 
AGAGAGCTGA AACGAGCTTC GAATGAAAGT GCGTCACAGT TCAATGATCA TCGACTCTTG 
CATAAAAGAT ACCTCATGCT GAACTTGCTC GGTAAGGGTG GATTCAGTGA GGTCTGGAAG 
GCGTTCGACA TTGAAGAGAA CCGGTATGTC GCATGCAAGA TCCATCATGT TAACAAAGAC 
TGGAAGGAGG AGAAGAAGGC GAACTACGTC AAGCACGCGA TGCGTGAAAA AGACATCCAC 
AAGTCATTGG ATCATTGCCG AATTGTGAAG CAATACGATC TGCTCACAAT CGACAATCAC 
TCGTTCTGCA CGGTTCTCGA ATATGTGCCG GGCAACGACC TCGATTTCTA CCTCAAGCAG 
AACAGGTCGA TCAGTGAGAA GGAGGCTCGA AGCATTATTA TGCAAGTTGT TTCTGCTCTT 
GTCTATCTGA ATGAAAAGAG CACTCCCATC ATTCATTATG ATCTAAAGCC TGCGAATATT 
CTTCTCGAGT CAGGAAACAC CAGCGGTGCA ATCAAGATCA CCGACTTTGG GCTGTCGAAA 
ATCATGGAGG GAGAGAGTGA CGATCATGAC TTGGGAATCG AGCTCACCTC ACAGTTCGCT 
GGAACTTATT GGTATCTTCC ACCTGAGACC TTCATCGTTC CACCACCAAA GATCACTTGT 
AAAGTCGATG TCTGGAGTAT TGGAGTCATC TTCTATCAAT GTATCTATGG AAAGAAGCCG 
TTCGGAAATG ATCTGACTCA ACAAAAGATC CTCGAATACA ATACGATTAT TAATGCAAGA 
GAAGTGTCAT TCCCAAGCAA GCCTCAGGTG AGCTCCGCTG CTCAAGATTT CATAAGAAGA 
TGCCTGCAGT ACAGGAAAGA AGACAGAGCA GATGTTTTCG AGCTGGCGAA GCATGAGCTT 
TTCAGACCAA GAGGAGCGAT AAGAGCCTCC GTTGCCGGAT CCGTAAGTTC TCCAAGCATT 
CCACGCAGTC CATCAGTGAA TCGTGAAGAT GACAATATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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tlk-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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