Tlk2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tlk2
Entrez Gene ID	303592
Full Name	tousled-like kinase 2
General protein information	Preferred Names tousled-like kinase 2 Names serine/threonine-protein kinase tousled-like 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	mouse homolog is a serine threonine kinase; may have a role in phosphorylations involved in regulating spermatogenesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191652.1	NP_001178581.1	serine/threonine-protein kinase tousled-like 2
NM_001191652.2	NP_001178581.1	serine/threonine-protein kinase tousled-like 2
XM_017597336.1	XP_017452825.1	serine/threonine-protein kinase tousled-like 2 isoform X5
XM_017597335.1	XP_017452824.1	serine/threonine-protein kinase tousled-like 2 isoform X4
XM_017597333.1	XP_017452822.1	serine/threonine-protein kinase tousled-like 2 isoform X1
XM_017597334.1	XP_017452823.1	serine/threonine-protein kinase tousled-like 2 isoform X2
XM_006247574.3	XP_006247636.1	serine/threonine-protein kinase tousled-like 2 isoform X3
XM_006247576.3	XP_006247638.1	serine/threonine-protein kinase tousled-like 2 isoform X3

Pathways from BioSystems

Homologies

	TLK2	XP_001107302.2
Canis lupus familiaris (dog)	TLK2	XP_005624288.1
Danio rerio (zebrafish)	tlk2	NP_001103887.1
Caenorhabditis elegans (roundworm)	tlk-1	NP_001040844.1
Xenopus tropicalis (tropical clawed frog)	tlk2	NP_001072711.1
Homo sapiens (human)	TLK2	NP_006843.2
Gallus gallus (chicken)	TLK2	XP_418070.3
Drosophila melanogaster (fruit fly)	tlk	NP_001138153.1
Mus musculus (house mouse)	Tlk2	NP_001106176.1
Pan troglodytes (chimpanzee)	TLK2	XP_523598.3
Rattus norvegicus (Norway rat)	Tlk2	NP_001178581.1
Bos taurus (cattle)	TLK2	NP_001179756.1

Gene Ontology
Bibliography

Related articles in PubMed

Caenorhabditis elegans TLK-1 controls cytokinesis by localizing AIR-2/Aurora B to midzone microtubules.
Yeh CH, Yang HJ, Lee IJ, Wu YC
Biochemical and biophysical research communications400(2)187-93(2010 Sep)

Phosphorylation-mediated control of histone chaperone ASF1 levels by Tousled-like kinases.
Pilyugin M, Demmers J, Verrijzer CP, Karch F, Moshkin YM
PloS one4(12)e8328(2009 Dec)

Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint.
Groth A, Lukas J, Nigg EA, Sillj? HH, Wernstedt C, Bartek J, Hansen K
The EMBO journal22(7)1676-87(2003 Apr)

Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication.
Sillj? HH, Takahashi K, Tanaka K, Van Houwe G, Nigg EA
The EMBO journal18(20)5691-702(1999 Oct)

Nuclear localization of protein kinase U-alpha is regulated by 14-3-3.
Zhang S, Xing H, Muslin AJ
The Journal of biological chemistry274(35)24865-72(1999 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tlk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tlk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa33848	NM_001191652.2 Latest version!	Rattus norvegicus tousled-like kinase 2 (Tlk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa33848	XM_006247576.3 Latest version!	Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa33848	XM_006247574.3 Latest version!	Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa46039	XM_017597333.1 Latest version!	Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa46040	XM_017597335.1 Latest version!	Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa57367	XM_017597334.1 Latest version!	Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa57368	XM_017597336.1 Latest version!	Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ORa33848	NM_001191652.1	Rattus norvegicus tousled-like kinase 2 (Tlk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tlk2 ( NM_001191652.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178581.1 Rattus norvegicus tousled-like kinase 2 (Tlk2), mRNA.

Price & Availability↑

CloneID	ORa33848
Clone ID Related Accession (Same CDS sequence)	NM_001191652.1 , NM_001191652.2 , XM_006247574.3 , XM_006247576.3
Accession Version	NM_001191652.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-08-14
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase tousled-like 2
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR03073174.1. On Jul 17, 2010 this sequence version replaced XM_221023.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT 
GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA 
CCGCGTCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GTACTGCAAA GGAGGCCTCA 
GAAGAGCAGT CTGCCCTGCC AACCCTCATG TCAGTGATGT TAGCAAAACC TCGACTTGAC 
ACAGAGCAGT TAGCGCCAAG GGGAGCTGGC CTCTGCTTCA CTTTCGTCTC TGCTCAACAA 
AACAGCCCTT CGTCCACGGG GTCTGGCAAT ACAGAACATT CTTGCAGCTC CCAGAAACAG 
ATCTCCATCC AGCACAGGCA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA 
GAAAACAGTA AGAACTCTGA CTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA 
GCCAACTGTG ATTTGAGACG ACAGATAGAT GAACAGCAAA AGATGCTAGA GAAATACAAG 
GAACGATTAA ATAGATGTGT CACCATGAGC AAGAAGCTCC TTATAGAAAA GTCAAAACAA 
GAGAAGATGG CATGCAGGGA CAAGAGCATG CAGGACCGAT TGCGATTAGG TCACTTTACA 
ACTGTCCGGC ATGGAGCCTC TTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAAAAC 
CTCATCAAGC AACAGGAAAG GATAAATTCA CAGAGAGAAG AGATAGAAAG GCAACGGAAA 
ATGTTAGCAA AACGGAAACC CCCTGCCATG GGTCAGGCCC CTCCCGCAAC CAATGAGCAG 
AAACAACGGA AAAGCAAGAC CAATGGAGCT GAAAATGAAA CGTTAACCTT AGCTGAATAC 
CATGAACAAG AAGAAATCTT CAAACTTAGA TTAGGTCATC TTAAGAAAGA GGAAGCAGAA 
ATCCAGGCAG AACTGGAAAG GCTAGAAAGG GTTAGGAATC TACACATCAG GGAATTAAAA 
AGGATACATA ATGAAGATAA TTCACAGTTT AAAGACCACC CAACACTAAA TGACAGATAT 
TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC GTTTGATCTA 
ACGGAGCAAA GATATGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG 
AAAAAGGAGA ATTACCACAA GCATGCGTGT AGGGAATACC GGATTCACAA AGAACTGGAT 
CACCCCAGAA TAGTGAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA 
GTATTAGAAT ACTGTGAAGG AAATGATCTG GACTTCTACC TAAAGCAGCA CAAATTAATG 
TCGGAGAAAG AAGCCCGATC CATTATTATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT 
GAAATAAAAC CTCCCATCAT ACACTATGAC CTCAAACCAG GTAATATCCT TTTAGTAAAT 
GGTACAGCGT GTGGAGAGAT AAAAATTACA GATTTTGGTC TTTCCAAGAT CATGGATGAC 
GATAGCTACA ATTCAGTGGA CGGCATGGAG CTAACGTCAC AAGGTGCTGG TACTTATTGG 
TATTTGCCAC CAGAGTGTTT TGTGGTTGGG AAAGAGCCAC CAAAGATCTC AAATAAAGTC 
GATGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTGT ATGGGAGGAA GCCTTTTGGT 
CATAACCAGT CCCAGCAAGA TATTCTACAA GAGAATACTA TTCTTAAAGC TACTGAAGTA 
CAGTTCCCGC CCAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATCAG GCGATGTTTG 
GCCTATCGAA AGGAAGACCG CATTGATGTG CAGCAGCTGG CCTGTGACCC CTACTTGTTG 
CCTCACATCC GAAAGTCAGT CTCCACAAGT AGTCCTGCAG GAGCTGCCAT TGCATCAACC 
TCGGGGGCAT CCAATAACAG TTCTTCGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178581.1
CDS	528..2780
Translation	MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSTAKEASEEQSALPTLMSVMLAKPRLDTEQLAPRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN

Target ORF information:

RefSeq Version	NM_001191652.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tousled-like kinase 2 (Tlk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191652.1

ORF Insert Sequence:

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT 
GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA 
CCGCGTCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GTACTGCAAA GGAGGCCTCA 
GAAGAGCAGT CTGCCCTGCC AACCCTCATG TCAGTGATGT TAGCAAAACC TCGACTTGAC 
ACAGAGCAGT TAGCGCCAAG GGGAGCTGGC CTCTGCTTCA CTTTCGTCTC TGCTCAACAA 
AACAGCCCTT CGTCCACGGG GTCTGGCAAT ACAGAACATT CTTGCAGCTC CCAGAAACAG 
ATCTCCATCC AGCACAGGCA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA 
GAAAACAGTA AGAACTCTGA CTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA 
GCCAACTGTG ATTTGAGACG ACAGATAGAT GAACAGCAAA AGATGCTAGA GAAATACAAG 
GAACGATTAA ATAGATGTGT CACCATGAGC AAGAAGCTCC TTATAGAAAA GTCAAAACAA 
GAGAAGATGG CATGCAGGGA CAAGAGCATG CAGGACCGAT TGCGATTAGG TCACTTTACA 
ACTGTCCGGC ATGGAGCCTC TTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAAAAC 
CTCATCAAGC AACAGGAAAG GATAAATTCA CAGAGAGAAG AGATAGAAAG GCAACGGAAA 
ATGTTAGCAA AACGGAAACC CCCTGCCATG GGTCAGGCCC CTCCCGCAAC CAATGAGCAG 
AAACAACGGA AAAGCAAGAC CAATGGAGCT GAAAATGAAA CGTTAACCTT AGCTGAATAC 
CATGAACAAG AAGAAATCTT CAAACTTAGA TTAGGTCATC TTAAGAAAGA GGAAGCAGAA 
ATCCAGGCAG AACTGGAAAG GCTAGAAAGG GTTAGGAATC TACACATCAG GGAATTAAAA 
AGGATACATA ATGAAGATAA TTCACAGTTT AAAGACCACC CAACACTAAA TGACAGATAT 
TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC GTTTGATCTA 
ACGGAGCAAA GATATGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG 
AAAAAGGAGA ATTACCACAA GCATGCGTGT AGGGAATACC GGATTCACAA AGAACTGGAT 
CACCCCAGAA TAGTGAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA 
GTATTAGAAT ACTGTGAAGG AAATGATCTG GACTTCTACC TAAAGCAGCA CAAATTAATG 
TCGGAGAAAG AAGCCCGATC CATTATTATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT 
GAAATAAAAC CTCCCATCAT ACACTATGAC CTCAAACCAG GTAATATCCT TTTAGTAAAT 
GGTACAGCGT GTGGAGAGAT AAAAATTACA GATTTTGGTC TTTCCAAGAT CATGGATGAC 
GATAGCTACA ATTCAGTGGA CGGCATGGAG CTAACGTCAC AAGGTGCTGG TACTTATTGG 
TATTTGCCAC CAGAGTGTTT TGTGGTTGGG AAAGAGCCAC CAAAGATCTC AAATAAAGTC 
GATGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTGT ATGGGAGGAA GCCTTTTGGT 
CATAACCAGT CCCAGCAAGA TATTCTACAA GAGAATACTA TTCTTAAAGC TACTGAAGTA 
CAGTTCCCGC CCAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATCAG GCGATGTTTG 
GCCTATCGAA AGGAAGACCG CATTGATGTG CAGCAGCTGG CCTGTGACCC CTACTTGTTG 
CCTCACATCC GAAAGTCAGT CTCCACAAGT AGTCCTGCAG GAGCTGCCAT TGCATCAACC 
TCGGGGGCAT CCAATAACAG TTCTTCGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tlk2 ( NM_001191652.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178581.1 Rattus norvegicus tousled-like kinase 2 (Tlk2), mRNA.

Price & Availability↑

CloneID	ORa33848
Clone ID Related Accession (Same CDS sequence)	NM_001191652.1 , NM_001191652.2 , XM_006247574.3 , XM_006247576.3
Accession Version	NM_001191652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase tousled-like 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07072195.1, FN804032.1, FM091827.1, FM098672.1, FM082999.1 and BC086544.1. On Aug 5, 2017 this sequence version replaced NM_001191652.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT 
GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA 
CCGCGTCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GTACTGCAAA GGAGGCCTCA 
GAAGAGCAGT CTGCCCTGCC AACCCTCATG TCAGTGATGT TAGCAAAACC TCGACTTGAC 
ACAGAGCAGT TAGCGCCAAG GGGAGCTGGC CTCTGCTTCA CTTTCGTCTC TGCTCAACAA 
AACAGCCCTT CGTCCACGGG GTCTGGCAAT ACAGAACATT CTTGCAGCTC CCAGAAACAG 
ATCTCCATCC AGCACAGGCA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA 
GAAAACAGTA AGAACTCTGA CTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA 
GCCAACTGTG ATTTGAGACG ACAGATAGAT GAACAGCAAA AGATGCTAGA GAAATACAAG 
GAACGATTAA ATAGATGTGT CACCATGAGC AAGAAGCTCC TTATAGAAAA GTCAAAACAA 
GAGAAGATGG CATGCAGGGA CAAGAGCATG CAGGACCGAT TGCGATTAGG TCACTTTACA 
ACTGTCCGGC ATGGAGCCTC TTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAAAAC 
CTCATCAAGC AACAGGAAAG GATAAATTCA CAGAGAGAAG AGATAGAAAG GCAACGGAAA 
ATGTTAGCAA AACGGAAACC CCCTGCCATG GGTCAGGCCC CTCCCGCAAC CAATGAGCAG 
AAACAACGGA AAAGCAAGAC CAATGGAGCT GAAAATGAAA CGTTAACCTT AGCTGAATAC 
CATGAACAAG AAGAAATCTT CAAACTTAGA TTAGGTCATC TTAAGAAAGA GGAAGCAGAA 
ATCCAGGCAG AACTGGAAAG GCTAGAAAGG GTTAGGAATC TACACATCAG GGAATTAAAA 
AGGATACATA ATGAAGATAA TTCACAGTTT AAAGACCACC CAACACTAAA TGACAGATAT 
TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC GTTTGATCTA 
ACGGAGCAAA GATATGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG 
AAAAAGGAGA ATTACCACAA GCATGCGTGT AGGGAATACC GGATTCACAA AGAACTGGAT 
CACCCCAGAA TAGTGAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA 
GTATTAGAAT ACTGTGAAGG AAATGATCTG GACTTCTACC TAAAGCAGCA CAAATTAATG 
TCGGAGAAAG AAGCCCGATC CATTATTATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT 
GAAATAAAAC CTCCCATCAT ACACTATGAC CTCAAACCAG GTAATATCCT TTTAGTAAAT 
GGTACAGCGT GTGGAGAGAT AAAAATTACA GATTTTGGTC TTTCCAAGAT CATGGATGAC 
GATAGCTACA ATTCAGTGGA CGGCATGGAG CTAACGTCAC AAGGTGCTGG TACTTATTGG 
TATTTGCCAC CAGAGTGTTT TGTGGTTGGG AAAGAGCCAC CAAAGATCTC AAATAAAGTC 
GATGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTGT ATGGGAGGAA GCCTTTTGGT 
CATAACCAGT CCCAGCAAGA TATTCTACAA GAGAATACTA TTCTTAAAGC TACTGAAGTA 
CAGTTCCCGC CCAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATCAG GCGATGTTTG 
GCCTATCGAA AGGAAGACCG CATTGATGTG CAGCAGCTGG CCTGTGACCC CTACTTGTTG 
CCTCACATCC GAAAGTCAGT CTCCACAAGT AGTCCTGCAG GAGCTGCCAT TGCATCAACC 
TCGGGGGCAT CCAATAACAG TTCTTCGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178581.1
CDS	525..2777
Translation	MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSTAKEASEEQSALPTLMSVMLAKPRLDTEQLAPRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN

Target ORF information:

RefSeq Version	NM_001191652.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tousled-like kinase 2 (Tlk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191652.2

ORF Insert Sequence:

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT 
GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA 
CCGCGTCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GTACTGCAAA GGAGGCCTCA 
GAAGAGCAGT CTGCCCTGCC AACCCTCATG TCAGTGATGT TAGCAAAACC TCGACTTGAC 
ACAGAGCAGT TAGCGCCAAG GGGAGCTGGC CTCTGCTTCA CTTTCGTCTC TGCTCAACAA 
AACAGCCCTT CGTCCACGGG GTCTGGCAAT ACAGAACATT CTTGCAGCTC CCAGAAACAG 
ATCTCCATCC AGCACAGGCA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA 
GAAAACAGTA AGAACTCTGA CTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA 
GCCAACTGTG ATTTGAGACG ACAGATAGAT GAACAGCAAA AGATGCTAGA GAAATACAAG 
GAACGATTAA ATAGATGTGT CACCATGAGC AAGAAGCTCC TTATAGAAAA GTCAAAACAA 
GAGAAGATGG CATGCAGGGA CAAGAGCATG CAGGACCGAT TGCGATTAGG TCACTTTACA 
ACTGTCCGGC ATGGAGCCTC TTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAAAAC 
CTCATCAAGC AACAGGAAAG GATAAATTCA CAGAGAGAAG AGATAGAAAG GCAACGGAAA 
ATGTTAGCAA AACGGAAACC CCCTGCCATG GGTCAGGCCC CTCCCGCAAC CAATGAGCAG 
AAACAACGGA AAAGCAAGAC CAATGGAGCT GAAAATGAAA CGTTAACCTT AGCTGAATAC 
CATGAACAAG AAGAAATCTT CAAACTTAGA TTAGGTCATC TTAAGAAAGA GGAAGCAGAA 
ATCCAGGCAG AACTGGAAAG GCTAGAAAGG GTTAGGAATC TACACATCAG GGAATTAAAA 
AGGATACATA ATGAAGATAA TTCACAGTTT AAAGACCACC CAACACTAAA TGACAGATAT 
TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC GTTTGATCTA 
ACGGAGCAAA GATATGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG 
AAAAAGGAGA ATTACCACAA GCATGCGTGT AGGGAATACC GGATTCACAA AGAACTGGAT 
CACCCCAGAA TAGTGAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA 
GTATTAGAAT ACTGTGAAGG AAATGATCTG GACTTCTACC TAAAGCAGCA CAAATTAATG 
TCGGAGAAAG AAGCCCGATC CATTATTATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT 
GAAATAAAAC CTCCCATCAT ACACTATGAC CTCAAACCAG GTAATATCCT TTTAGTAAAT 
GGTACAGCGT GTGGAGAGAT AAAAATTACA GATTTTGGTC TTTCCAAGAT CATGGATGAC 
GATAGCTACA ATTCAGTGGA CGGCATGGAG CTAACGTCAC AAGGTGCTGG TACTTATTGG 
TATTTGCCAC CAGAGTGTTT TGTGGTTGGG AAAGAGCCAC CAAAGATCTC AAATAAAGTC 
GATGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTGT ATGGGAGGAA GCCTTTTGGT 
CATAACCAGT CCCAGCAAGA TATTCTACAA GAGAATACTA TTCTTAAAGC TACTGAAGTA 
CAGTTCCCGC CCAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATCAG GCGATGTTTG 
GCCTATCGAA AGGAAGACCG CATTGATGTG CAGCAGCTGG CCTGTGACCC CTACTTGTTG 
CCTCACATCC GAAAGTCAGT CTCCACAAGT AGTCCTGCAG GAGCTGCCAT TGCATCAACC 
TCGGGGGCAT CCAATAACAG TTCTTCGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tlk2 ( XM_006247574.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247636.1 Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa33848
Clone ID Related Accession (Same CDS sequence)	NM_001191652.1 , NM_001191652.2 , XM_006247574.3 , XM_006247576.3
Accession Version	XM_006247574.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase tousled-like 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247574.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT 
GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA 
CCGCGTCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GTACTGCAAA GGAGGCCTCA 
GAAGAGCAGT CTGCCCTGCC AACCCTCATG TCAGTGATGT TAGCAAAACC TCGACTTGAC 
ACAGAGCAGT TAGCGCCAAG GGGAGCTGGC CTCTGCTTCA CTTTCGTCTC TGCTCAACAA 
AACAGCCCTT CGTCCACGGG GTCTGGCAAT ACAGAACATT CTTGCAGCTC CCAGAAACAG 
ATCTCCATCC AGCACAGGCA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA 
GAAAACAGTA AGAACTCTGA CTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA 
GCCAACTGTG ATTTGAGACG ACAGATAGAT GAACAGCAAA AGATGCTAGA GAAATACAAG 
GAACGATTAA ATAGATGTGT CACCATGAGC AAGAAGCTCC TTATAGAAAA GTCAAAACAA 
GAGAAGATGG CATGCAGGGA CAAGAGCATG CAGGACCGAT TGCGATTAGG TCACTTTACA 
ACTGTCCGGC ATGGAGCCTC TTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAAAAC 
CTCATCAAGC AACAGGAAAG GATAAATTCA CAGAGAGAAG AGATAGAAAG GCAACGGAAA 
ATGTTAGCAA AACGGAAACC CCCTGCCATG GGTCAGGCCC CTCCCGCAAC CAATGAGCAG 
AAACAACGGA AAAGCAAGAC CAATGGAGCT GAAAATGAAA CGTTAACCTT AGCTGAATAC 
CATGAACAAG AAGAAATCTT CAAACTTAGA TTAGGTCATC TTAAGAAAGA GGAAGCAGAA 
ATCCAGGCAG AACTGGAAAG GCTAGAAAGG GTTAGGAATC TACACATCAG GGAATTAAAA 
AGGATACATA ATGAAGATAA TTCACAGTTT AAAGACCACC CAACACTAAA TGACAGATAT 
TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC GTTTGATCTA 
ACGGAGCAAA GATATGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG 
AAAAAGGAGA ATTACCACAA GCATGCGTGT AGGGAATACC GGATTCACAA AGAACTGGAT 
CACCCCAGAA TAGTGAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA 
GTATTAGAAT ACTGTGAAGG AAATGATCTG GACTTCTACC TAAAGCAGCA CAAATTAATG 
TCGGAGAAAG AAGCCCGATC CATTATTATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT 
GAAATAAAAC CTCCCATCAT ACACTATGAC CTCAAACCAG GTAATATCCT TTTAGTAAAT 
GGTACAGCGT GTGGAGAGAT AAAAATTACA GATTTTGGTC TTTCCAAGAT CATGGATGAC 
GATAGCTACA ATTCAGTGGA CGGCATGGAG CTAACGTCAC AAGGTGCTGG TACTTATTGG 
TATTTGCCAC CAGAGTGTTT TGTGGTTGGG AAAGAGCCAC CAAAGATCTC AAATAAAGTC 
GATGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTGT ATGGGAGGAA GCCTTTTGGT 
CATAACCAGT CCCAGCAAGA TATTCTACAA GAGAATACTA TTCTTAAAGC TACTGAAGTA 
CAGTTCCCGC CCAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATCAG GCGATGTTTG 
GCCTATCGAA AGGAAGACCG CATTGATGTG CAGCAGCTGG CCTGTGACCC CTACTTGTTG 
CCTCACATCC GAAAGTCAGT CTCCACAAGT AGTCCTGCAG GAGCTGCCAT TGCATCAACC 
TCGGGGGCAT CCAATAACAG TTCTTCGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247636.1
CDS	4274..6526
Translation	MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSTAKEASEEQSALPTLMSVMLAKPRLDTEQLAPRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN

Target ORF information:

RefSeq Version	XM_006247574.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247574.3

ORF Insert Sequence:

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT 
GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA 
CCGCGTCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GTACTGCAAA GGAGGCCTCA 
GAAGAGCAGT CTGCCCTGCC AACCCTCATG TCAGTGATGT TAGCAAAACC TCGACTTGAC 
ACAGAGCAGT TAGCGCCAAG GGGAGCTGGC CTCTGCTTCA CTTTCGTCTC TGCTCAACAA 
AACAGCCCTT CGTCCACGGG GTCTGGCAAT ACAGAACATT CTTGCAGCTC CCAGAAACAG 
ATCTCCATCC AGCACAGGCA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA 
GAAAACAGTA AGAACTCTGA CTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA 
GCCAACTGTG ATTTGAGACG ACAGATAGAT GAACAGCAAA AGATGCTAGA GAAATACAAG 
GAACGATTAA ATAGATGTGT CACCATGAGC AAGAAGCTCC TTATAGAAAA GTCAAAACAA 
GAGAAGATGG CATGCAGGGA CAAGAGCATG CAGGACCGAT TGCGATTAGG TCACTTTACA 
ACTGTCCGGC ATGGAGCCTC TTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAAAAC 
CTCATCAAGC AACAGGAAAG GATAAATTCA CAGAGAGAAG AGATAGAAAG GCAACGGAAA 
ATGTTAGCAA AACGGAAACC CCCTGCCATG GGTCAGGCCC CTCCCGCAAC CAATGAGCAG 
AAACAACGGA AAAGCAAGAC CAATGGAGCT GAAAATGAAA CGTTAACCTT AGCTGAATAC 
CATGAACAAG AAGAAATCTT CAAACTTAGA TTAGGTCATC TTAAGAAAGA GGAAGCAGAA 
ATCCAGGCAG AACTGGAAAG GCTAGAAAGG GTTAGGAATC TACACATCAG GGAATTAAAA 
AGGATACATA ATGAAGATAA TTCACAGTTT AAAGACCACC CAACACTAAA TGACAGATAT 
TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC GTTTGATCTA 
ACGGAGCAAA GATATGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG 
AAAAAGGAGA ATTACCACAA GCATGCGTGT AGGGAATACC GGATTCACAA AGAACTGGAT 
CACCCCAGAA TAGTGAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA 
GTATTAGAAT ACTGTGAAGG AAATGATCTG GACTTCTACC TAAAGCAGCA CAAATTAATG 
TCGGAGAAAG AAGCCCGATC CATTATTATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT 
GAAATAAAAC CTCCCATCAT ACACTATGAC CTCAAACCAG GTAATATCCT TTTAGTAAAT 
GGTACAGCGT GTGGAGAGAT AAAAATTACA GATTTTGGTC TTTCCAAGAT CATGGATGAC 
GATAGCTACA ATTCAGTGGA CGGCATGGAG CTAACGTCAC AAGGTGCTGG TACTTATTGG 
TATTTGCCAC CAGAGTGTTT TGTGGTTGGG AAAGAGCCAC CAAAGATCTC AAATAAAGTC 
GATGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTGT ATGGGAGGAA GCCTTTTGGT 
CATAACCAGT CCCAGCAAGA TATTCTACAA GAGAATACTA TTCTTAAAGC TACTGAAGTA 
CAGTTCCCGC CCAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATCAG GCGATGTTTG 
GCCTATCGAA AGGAAGACCG CATTGATGTG CAGCAGCTGG CCTGTGACCC CTACTTGTTG 
CCTCACATCC GAAAGTCAGT CTCCACAAGT AGTCCTGCAG GAGCTGCCAT TGCATCAACC 
TCGGGGGCAT CCAATAACAG TTCTTCGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tlk2 ( XM_006247576.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247638.1 Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa33848
Clone ID Related Accession (Same CDS sequence)	NM_001191652.1 , NM_001191652.2 , XM_006247574.3 , XM_006247576.3
Accession Version	XM_006247576.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase tousled-like 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247576.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT 
GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA 
CCGCGTCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GTACTGCAAA GGAGGCCTCA 
GAAGAGCAGT CTGCCCTGCC AACCCTCATG TCAGTGATGT TAGCAAAACC TCGACTTGAC 
ACAGAGCAGT TAGCGCCAAG GGGAGCTGGC CTCTGCTTCA CTTTCGTCTC TGCTCAACAA 
AACAGCCCTT CGTCCACGGG GTCTGGCAAT ACAGAACATT CTTGCAGCTC CCAGAAACAG 
ATCTCCATCC AGCACAGGCA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA 
GAAAACAGTA AGAACTCTGA CTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA 
GCCAACTGTG ATTTGAGACG ACAGATAGAT GAACAGCAAA AGATGCTAGA GAAATACAAG 
GAACGATTAA ATAGATGTGT CACCATGAGC AAGAAGCTCC TTATAGAAAA GTCAAAACAA 
GAGAAGATGG CATGCAGGGA CAAGAGCATG CAGGACCGAT TGCGATTAGG TCACTTTACA 
ACTGTCCGGC ATGGAGCCTC TTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAAAAC 
CTCATCAAGC AACAGGAAAG GATAAATTCA CAGAGAGAAG AGATAGAAAG GCAACGGAAA 
ATGTTAGCAA AACGGAAACC CCCTGCCATG GGTCAGGCCC CTCCCGCAAC CAATGAGCAG 
AAACAACGGA AAAGCAAGAC CAATGGAGCT GAAAATGAAA CGTTAACCTT AGCTGAATAC 
CATGAACAAG AAGAAATCTT CAAACTTAGA TTAGGTCATC TTAAGAAAGA GGAAGCAGAA 
ATCCAGGCAG AACTGGAAAG GCTAGAAAGG GTTAGGAATC TACACATCAG GGAATTAAAA 
AGGATACATA ATGAAGATAA TTCACAGTTT AAAGACCACC CAACACTAAA TGACAGATAT 
TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC GTTTGATCTA 
ACGGAGCAAA GATATGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG 
AAAAAGGAGA ATTACCACAA GCATGCGTGT AGGGAATACC GGATTCACAA AGAACTGGAT 
CACCCCAGAA TAGTGAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA 
GTATTAGAAT ACTGTGAAGG AAATGATCTG GACTTCTACC TAAAGCAGCA CAAATTAATG 
TCGGAGAAAG AAGCCCGATC CATTATTATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT 
GAAATAAAAC CTCCCATCAT ACACTATGAC CTCAAACCAG GTAATATCCT TTTAGTAAAT 
GGTACAGCGT GTGGAGAGAT AAAAATTACA GATTTTGGTC TTTCCAAGAT CATGGATGAC 
GATAGCTACA ATTCAGTGGA CGGCATGGAG CTAACGTCAC AAGGTGCTGG TACTTATTGG 
TATTTGCCAC CAGAGTGTTT TGTGGTTGGG AAAGAGCCAC CAAAGATCTC AAATAAAGTC 
GATGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTGT ATGGGAGGAA GCCTTTTGGT 
CATAACCAGT CCCAGCAAGA TATTCTACAA GAGAATACTA TTCTTAAAGC TACTGAAGTA 
CAGTTCCCGC CCAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATCAG GCGATGTTTG 
GCCTATCGAA AGGAAGACCG CATTGATGTG CAGCAGCTGG CCTGTGACCC CTACTTGTTG 
CCTCACATCC GAAAGTCAGT CTCCACAAGT AGTCCTGCAG GAGCTGCCAT TGCATCAACC 
TCGGGGGCAT CCAATAACAG TTCTTCGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247638.1
CDS	125..2377
Translation	MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSTAKEASEEQSALPTLMSVMLAKPRLDTEQLAPRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN

Target ORF information:

RefSeq Version	XM_006247576.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247576.3

ORF Insert Sequence:

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT 
GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA 
CCGCGTCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GTACTGCAAA GGAGGCCTCA 
GAAGAGCAGT CTGCCCTGCC AACCCTCATG TCAGTGATGT TAGCAAAACC TCGACTTGAC 
ACAGAGCAGT TAGCGCCAAG GGGAGCTGGC CTCTGCTTCA CTTTCGTCTC TGCTCAACAA 
AACAGCCCTT CGTCCACGGG GTCTGGCAAT ACAGAACATT CTTGCAGCTC CCAGAAACAG 
ATCTCCATCC AGCACAGGCA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA 
GAAAACAGTA AGAACTCTGA CTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA 
GCCAACTGTG ATTTGAGACG ACAGATAGAT GAACAGCAAA AGATGCTAGA GAAATACAAG 
GAACGATTAA ATAGATGTGT CACCATGAGC AAGAAGCTCC TTATAGAAAA GTCAAAACAA 
GAGAAGATGG CATGCAGGGA CAAGAGCATG CAGGACCGAT TGCGATTAGG TCACTTTACA 
ACTGTCCGGC ATGGAGCCTC TTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAAAAC 
CTCATCAAGC AACAGGAAAG GATAAATTCA CAGAGAGAAG AGATAGAAAG GCAACGGAAA 
ATGTTAGCAA AACGGAAACC CCCTGCCATG GGTCAGGCCC CTCCCGCAAC CAATGAGCAG 
AAACAACGGA AAAGCAAGAC CAATGGAGCT GAAAATGAAA CGTTAACCTT AGCTGAATAC 
CATGAACAAG AAGAAATCTT CAAACTTAGA TTAGGTCATC TTAAGAAAGA GGAAGCAGAA 
ATCCAGGCAG AACTGGAAAG GCTAGAAAGG GTTAGGAATC TACACATCAG GGAATTAAAA 
AGGATACATA ATGAAGATAA TTCACAGTTT AAAGACCACC CAACACTAAA TGACAGATAT 
TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC GTTTGATCTA 
ACGGAGCAAA GATATGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG 
AAAAAGGAGA ATTACCACAA GCATGCGTGT AGGGAATACC GGATTCACAA AGAACTGGAT 
CACCCCAGAA TAGTGAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA 
GTATTAGAAT ACTGTGAAGG AAATGATCTG GACTTCTACC TAAAGCAGCA CAAATTAATG 
TCGGAGAAAG AAGCCCGATC CATTATTATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT 
GAAATAAAAC CTCCCATCAT ACACTATGAC CTCAAACCAG GTAATATCCT TTTAGTAAAT 
GGTACAGCGT GTGGAGAGAT AAAAATTACA GATTTTGGTC TTTCCAAGAT CATGGATGAC 
GATAGCTACA ATTCAGTGGA CGGCATGGAG CTAACGTCAC AAGGTGCTGG TACTTATTGG 
TATTTGCCAC CAGAGTGTTT TGTGGTTGGG AAAGAGCCAC CAAAGATCTC AAATAAAGTC 
GATGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTGT ATGGGAGGAA GCCTTTTGGT 
CATAACCAGT CCCAGCAAGA TATTCTACAA GAGAATACTA TTCTTAAAGC TACTGAAGTA 
CAGTTCCCGC CCAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATCAG GCGATGTTTG 
GCCTATCGAA AGGAAGACCG CATTGATGTG CAGCAGCTGG CCTGTGACCC CTACTTGTTG 
CCTCACATCC GAAAGTCAGT CTCCACAAGT AGTCCTGCAG GAGCTGCCAT TGCATCAACC 
TCGGGGGCAT CCAATAACAG TTCTTCGAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tlk2 ( XM_017597333.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452822.1 Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa46039
Clone ID Related Accession (Same CDS sequence)	XM_017597333.1
Accession Version	XM_017597333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase tousled-like 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGCGT CGAGTAGAAC AGCCTCTCTA TGGTTTAGAT 
GGCAGTACTG CAAAGGAGGC CTCAGAAGAG CAGTCTGCCC TGCCAACCCT CATGTCAGTG 
ATGTTAGCAA AACCTCGACT TGACACAGAG CAGTTAGCGC CAAGGGGAGC TGGCCTCTGC 
TTCACTTTCG TCTCTGCTCA ACAAAACAGC CCTTCGTCCA CGGGGTCTGG CAATACAGAA 
CATTCTTGCA GCTCCCAGAA ACAGATCTCC ATCCAGCACA GGCAGACCCA GTCTGACCTC 
ACAATAGAAA AAATATCTGC ACTAGAAAAC AGTAAGAACT CTGACTTAGA GAAGAAGGAA 
GGAAGAATAG ATGATTTATT AAGAGCCAAC TGTGATTTGA GACGACAGAT AGATGAACAG 
CAAAAGATGC TAGAGAAATA CAAGGAACGA TTAAATAGAT GTGTCACCAT GAGCAAGAAG 
CTCCTTATAG AAAAGTCAAA ACAAGAGAAG ATGGCATGCA GGGACAAGAG CATGCAGGAC 
CGATTGCGAT TAGGTCACTT TACAACTGTC CGGCATGGAG CCTCTTTTAC TGAACAGTGG 
ACAGATGGTT ATGCTTTCCA AAACCTCATC AAGCAACAGG AAAGGATAAA TTCACAGAGA 
GAAGAGATAG AAAGGCAACG GAAAATGTTA GCAAAACGGA AACCCCCTGC CATGGGTCAG 
GCCCCTCCCG CAACCAATGA GCAGAAACAA CGGAAAAGCA AGACCAATGG AGCTGAAAAT 
GAAACGTTAA CCTTAGCTGA ATACCATGAA CAAGAAGAAA TCTTCAAACT TAGATTAGGT 
CATCTTAAGA AAGAGGAAGC AGAAATCCAG GCAGAACTGG AAAGGCTAGA AAGGGTTAGG 
AATCTACACA TCAGGGAATT AAAAAGGATA CATAATGAAG ATAATTCACA GTTTAAAGAC 
CACCCAACAC TAAATGACAG ATATTTGTTG TTACATCTTT TGGGTAGAGG AGGTTTCAGT 
GAAGTTTACA AGGCGTTTGA TCTAACGGAG CAAAGATATG TAGCTGTGAA AATTCACCAG 
TTAAATAAAA ACTGGAGAGA TGAGAAAAAG GAGAATTACC ACAAGCATGC GTGTAGGGAA 
TACCGGATTC ACAAAGAACT GGATCACCCC AGAATAGTGA AGCTGTATGA TTACTTTTCA 
CTGGACACTG ACTCGTTTTG TACAGTATTA GAATACTGTG AAGGAAATGA TCTGGACTTC 
TACCTAAAGC AGCACAAATT AATGTCGGAG AAAGAAGCCC GATCCATTAT TATGCAGATT 
GTGAATGCTT TAAAGTACTT AAATGAAATA AAACCTCCCA TCATACACTA TGACCTCAAA 
CCAGGTAATA TCCTTTTAGT AAATGGTACA GCGTGTGGAG AGATAAAAAT TACAGATTTT 
GGTCTTTCCA AGATCATGGA TGACGATAGC TACAATTCAG TGGACGGCAT GGAGCTAACG 
TCACAAGGTG CTGGTACTTA TTGGTATTTG CCACCAGAGT GTTTTGTGGT TGGGAAAGAG 
CCACCAAAGA TCTCAAATAA AGTCGATGTC TGGTCAGTGG GTGTGATCTT CTACCAGTGT 
CTGTATGGGA GGAAGCCTTT TGGTCATAAC CAGTCCCAGC AAGATATTCT ACAAGAGAAT 
ACTATTCTTA AAGCTACTGA AGTACAGTTC CCGCCCAAGC CAGTAGTAAC ACCTGAAGCA 
AAGGCGTTTA TCAGGCGATG TTTGGCCTAT CGAAAGGAAG ACCGCATTGA TGTGCAGCAG 
CTGGCCTGTG ACCCCTACTT GTTGCCTCAC ATCCGAAAGT CAGTCTCCAC AAGTAGTCCT 
GCAGGAGCTG CCATTGCATC AACCTCGGGG GCATCCAATA ACAGTTCTTC GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452822.1
CDS	3..2159
Translation	MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFERRVEQPLYGLDGSTAKEASEEQSALPTLMSVMLAKPRLDTEQLAPRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN

Target ORF information:

RefSeq Version	XM_017597333.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597333.1

ORF Insert Sequence:

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGCGT CGAGTAGAAC AGCCTCTCTA TGGTTTAGAT 
GGCAGTACTG CAAAGGAGGC CTCAGAAGAG CAGTCTGCCC TGCCAACCCT CATGTCAGTG 
ATGTTAGCAA AACCTCGACT TGACACAGAG CAGTTAGCGC CAAGGGGAGC TGGCCTCTGC 
TTCACTTTCG TCTCTGCTCA ACAAAACAGC CCTTCGTCCA CGGGGTCTGG CAATACAGAA 
CATTCTTGCA GCTCCCAGAA ACAGATCTCC ATCCAGCACA GGCAGACCCA GTCTGACCTC 
ACAATAGAAA AAATATCTGC ACTAGAAAAC AGTAAGAACT CTGACTTAGA GAAGAAGGAA 
GGAAGAATAG ATGATTTATT AAGAGCCAAC TGTGATTTGA GACGACAGAT AGATGAACAG 
CAAAAGATGC TAGAGAAATA CAAGGAACGA TTAAATAGAT GTGTCACCAT GAGCAAGAAG 
CTCCTTATAG AAAAGTCAAA ACAAGAGAAG ATGGCATGCA GGGACAAGAG CATGCAGGAC 
CGATTGCGAT TAGGTCACTT TACAACTGTC CGGCATGGAG CCTCTTTTAC TGAACAGTGG 
ACAGATGGTT ATGCTTTCCA AAACCTCATC AAGCAACAGG AAAGGATAAA TTCACAGAGA 
GAAGAGATAG AAAGGCAACG GAAAATGTTA GCAAAACGGA AACCCCCTGC CATGGGTCAG 
GCCCCTCCCG CAACCAATGA GCAGAAACAA CGGAAAAGCA AGACCAATGG AGCTGAAAAT 
GAAACGTTAA CCTTAGCTGA ATACCATGAA CAAGAAGAAA TCTTCAAACT TAGATTAGGT 
CATCTTAAGA AAGAGGAAGC AGAAATCCAG GCAGAACTGG AAAGGCTAGA AAGGGTTAGG 
AATCTACACA TCAGGGAATT AAAAAGGATA CATAATGAAG ATAATTCACA GTTTAAAGAC 
CACCCAACAC TAAATGACAG ATATTTGTTG TTACATCTTT TGGGTAGAGG AGGTTTCAGT 
GAAGTTTACA AGGCGTTTGA TCTAACGGAG CAAAGATATG TAGCTGTGAA AATTCACCAG 
TTAAATAAAA ACTGGAGAGA TGAGAAAAAG GAGAATTACC ACAAGCATGC GTGTAGGGAA 
TACCGGATTC ACAAAGAACT GGATCACCCC AGAATAGTGA AGCTGTATGA TTACTTTTCA 
CTGGACACTG ACTCGTTTTG TACAGTATTA GAATACTGTG AAGGAAATGA TCTGGACTTC 
TACCTAAAGC AGCACAAATT AATGTCGGAG AAAGAAGCCC GATCCATTAT TATGCAGATT 
GTGAATGCTT TAAAGTACTT AAATGAAATA AAACCTCCCA TCATACACTA TGACCTCAAA 
CCAGGTAATA TCCTTTTAGT AAATGGTACA GCGTGTGGAG AGATAAAAAT TACAGATTTT 
GGTCTTTCCA AGATCATGGA TGACGATAGC TACAATTCAG TGGACGGCAT GGAGCTAACG 
TCACAAGGTG CTGGTACTTA TTGGTATTTG CCACCAGAGT GTTTTGTGGT TGGGAAAGAG 
CCACCAAAGA TCTCAAATAA AGTCGATGTC TGGTCAGTGG GTGTGATCTT CTACCAGTGT 
CTGTATGGGA GGAAGCCTTT TGGTCATAAC CAGTCCCAGC AAGATATTCT ACAAGAGAAT 
ACTATTCTTA AAGCTACTGA AGTACAGTTC CCGCCCAAGC CAGTAGTAAC ACCTGAAGCA 
AAGGCGTTTA TCAGGCGATG TTTGGCCTAT CGAAAGGAAG ACCGCATTGA TGTGCAGCAG 
CTGGCCTGTG ACCCCTACTT GTTGCCTCAC ATCCGAAAGT CAGTCTCCAC AAGTAGTCCT 
GCAGGAGCTG CCATTGCATC AACCTCGGGG GCATCCAATA ACAGTTCTTC GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tlk2 ( XM_017597335.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452824.1 Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa46040
Clone ID Related Accession (Same CDS sequence)	XM_017597335.1
Accession Version	XM_017597335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase tousled-like 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT 
GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA 
CCGCGTCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GTACTGCAAA GGAGGCCTCA 
GAAGAGCAGT CTGCCCTGCC AACCCTCATG TCAGTGATGT TAGCAAAACC TCGACTTGAC 
ACAGAGCAGT TAGCGCCAAG GGGAGCTGGC CTCTGCTTCA CTTTCGTCTC TGCCAACTGT 
GATTTGAGAC GACAGATAGA TGAACAGCAA AAGATGCTAG AGAAATACAA GGAACGATTA 
AATAGATGTG TCACCATGAG CAAGAAGCTC CTTATAGAAA AGTCAAAACA AGAGAAGATG 
GCATGCAGGG ACAAGAGCAT GCAGGACCGA TTGCGATTAG GTCACTTTAC AACTGTCCGG 
CATGGAGCCT CTTTTACTGA ACAGTGGACA GATGGTTATG CTTTCCAAAA CCTCATCAAG 
CAACAGGAAA GGATAAATTC ACAGAGAGAA GAGATAGAAA GGCAACGGAA AATGTTAGCA 
AAACGGAAAC CCCCTGCCAT GGGTCAGGCC CCTCCCGCAA CCAATGAGCA GAAACAACGG 
AAAAGCAAGA CCAATGGAGC TGAAAATGAA ACGTTAACCT TAGCTGAATA CCATGAACAA 
GAAGAAATCT TCAAACTTAG ATTAGGTCAT CTTAAGAAAG AGGAAGCAGA AATCCAGGCA 
GAACTGGAAA GGCTAGAAAG GGTTAGGAAT CTACACATCA GGGAATTAAA AAGGATACAT 
AATGAAGATA ATTCACAGTT TAAAGACCAC CCAACACTAA ATGACAGATA TTTGTTGTTA 
CATCTTTTGG GTAGAGGAGG TTTCAGTGAA GTTTACAAGG CGTTTGATCT AACGGAGCAA 
AGATATGTAG CTGTGAAAAT TCACCAGTTA AATAAAAACT GGAGAGATGA GAAAAAGGAG 
AATTACCACA AGCATGCGTG TAGGGAATAC CGGATTCACA AAGAACTGGA TCACCCCAGA 
ATAGTGAAGC TGTATGATTA CTTTTCACTG GACACTGACT CGTTTTGTAC AGTATTAGAA 
TACTGTGAAG GAAATGATCT GGACTTCTAC CTAAAGCAGC ACAAATTAAT GTCGGAGAAA 
GAAGCCCGAT CCATTATTAT GCAGATTGTG AATGCTTTAA AGTACTTAAA TGAAATAAAA 
CCTCCCATCA TACACTATGA CCTCAAACCA GGTAATATCC TTTTAGTAAA TGGTACAGCG 
TGTGGAGAGA TAAAAATTAC AGATTTTGGT CTTTCCAAGA TCATGGATGA CGATAGCTAC 
AATTCAGTGG ACGGCATGGA GCTAACGTCA CAAGGTGCTG GTACTTATTG GTATTTGCCA 
CCAGAGTGTT TTGTGGTTGG GAAAGAGCCA CCAAAGATCT CAAATAAAGT CGATGTCTGG 
TCAGTGGGTG TGATCTTCTA CCAGTGTCTG TATGGGAGGA AGCCTTTTGG TCATAACCAG 
TCCCAGCAAG ATATTCTACA AGAGAATACT ATTCTTAAAG CTACTGAAGT ACAGTTCCCG 
CCCAAGCCAG TAGTAACACC TGAAGCAAAG GCGTTTATCA GGCGATGTTT GGCCTATCGA 
AAGGAAGACC GCATTGATGT GCAGCAGCTG GCCTGTGACC CCTACTTGTT GCCTCACATC 
CGAAAGTCAG TCTCCACAAG TAGTCCTGCA GGAGCTGCCA TTGCATCAAC CTCGGGGGCA 
TCCAATAACA GTTCTTCGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452824.1
CDS	3..2066
Translation	MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSTAKEASEEQSALPTLMSVMLAKPRLDTEQLAPRGAGLCFTFVSANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN

Target ORF information:

RefSeq Version	XM_017597335.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597335.1

ORF Insert Sequence:

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT 
GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA 
CCGCGTCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GTACTGCAAA GGAGGCCTCA 
GAAGAGCAGT CTGCCCTGCC AACCCTCATG TCAGTGATGT TAGCAAAACC TCGACTTGAC 
ACAGAGCAGT TAGCGCCAAG GGGAGCTGGC CTCTGCTTCA CTTTCGTCTC TGCCAACTGT 
GATTTGAGAC GACAGATAGA TGAACAGCAA AAGATGCTAG AGAAATACAA GGAACGATTA 
AATAGATGTG TCACCATGAG CAAGAAGCTC CTTATAGAAA AGTCAAAACA AGAGAAGATG 
GCATGCAGGG ACAAGAGCAT GCAGGACCGA TTGCGATTAG GTCACTTTAC AACTGTCCGG 
CATGGAGCCT CTTTTACTGA ACAGTGGACA GATGGTTATG CTTTCCAAAA CCTCATCAAG 
CAACAGGAAA GGATAAATTC ACAGAGAGAA GAGATAGAAA GGCAACGGAA AATGTTAGCA 
AAACGGAAAC CCCCTGCCAT GGGTCAGGCC CCTCCCGCAA CCAATGAGCA GAAACAACGG 
AAAAGCAAGA CCAATGGAGC TGAAAATGAA ACGTTAACCT TAGCTGAATA CCATGAACAA 
GAAGAAATCT TCAAACTTAG ATTAGGTCAT CTTAAGAAAG AGGAAGCAGA AATCCAGGCA 
GAACTGGAAA GGCTAGAAAG GGTTAGGAAT CTACACATCA GGGAATTAAA AAGGATACAT 
AATGAAGATA ATTCACAGTT TAAAGACCAC CCAACACTAA ATGACAGATA TTTGTTGTTA 
CATCTTTTGG GTAGAGGAGG TTTCAGTGAA GTTTACAAGG CGTTTGATCT AACGGAGCAA 
AGATATGTAG CTGTGAAAAT TCACCAGTTA AATAAAAACT GGAGAGATGA GAAAAAGGAG 
AATTACCACA AGCATGCGTG TAGGGAATAC CGGATTCACA AAGAACTGGA TCACCCCAGA 
ATAGTGAAGC TGTATGATTA CTTTTCACTG GACACTGACT CGTTTTGTAC AGTATTAGAA 
TACTGTGAAG GAAATGATCT GGACTTCTAC CTAAAGCAGC ACAAATTAAT GTCGGAGAAA 
GAAGCCCGAT CCATTATTAT GCAGATTGTG AATGCTTTAA AGTACTTAAA TGAAATAAAA 
CCTCCCATCA TACACTATGA CCTCAAACCA GGTAATATCC TTTTAGTAAA TGGTACAGCG 
TGTGGAGAGA TAAAAATTAC AGATTTTGGT CTTTCCAAGA TCATGGATGA CGATAGCTAC 
AATTCAGTGG ACGGCATGGA GCTAACGTCA CAAGGTGCTG GTACTTATTG GTATTTGCCA 
CCAGAGTGTT TTGTGGTTGG GAAAGAGCCA CCAAAGATCT CAAATAAAGT CGATGTCTGG 
TCAGTGGGTG TGATCTTCTA CCAGTGTCTG TATGGGAGGA AGCCTTTTGG TCATAACCAG 
TCCCAGCAAG ATATTCTACA AGAGAATACT ATTCTTAAAG CTACTGAAGT ACAGTTCCCG 
CCCAAGCCAG TAGTAACACC TGAAGCAAAG GCGTTTATCA GGCGATGTTT GGCCTATCGA 
AAGGAAGACC GCATTGATGT GCAGCAGCTG GCCTGTGACC CCTACTTGTT GCCTCACATC 
CGAAAGTCAG TCTCCACAAG TAGTCCTGCA GGAGCTGCCA TTGCATCAAC CTCGGGGGCA 
TCCAATAACA GTTCTTCGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tlk2 ( XM_017597334.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452823.1 Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa57367
Clone ID Related Accession (Same CDS sequence)	XM_017597334.1
Accession Version	XM_017597334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase tousled-like 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT 
GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA 
CCGCGTCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GTACTGCAAA GGAGGCCTCA 
GAAGAGCAGT CTGCCCTGCC AACCCTCATG TCAGTGATGT TAGCAAAACC TCGACTTGAC 
ACAGAGCAGT TAGCGCCAAG GGGAGCTGGC CTCTGCTTCA CTTTCGTCTC TGCTCAACAA 
AACAGCCCTT CGTCCACGGG GTCTGGCAAT ACAGAACATT CTTGCAGCTC CCAGAAACAG 
ATCTCCATCC AGCACAGGCA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA 
GAAAACAGTA AGAACTCTGA CTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA 
TCAAAACAAG AGAAGATGGC ATGCAGGGAC AAGAGCATGC AGGACCGATT GCGATTAGGT 
CACTTTACAA CTGTCCGGCA TGGAGCCTCT TTTACTGAAC AGTGGACAGA TGGTTATGCT 
TTCCAAAACC TCATCAAGCA ACAGGAAAGG ATAAATTCAC AGAGAGAAGA GATAGAAAGG 
CAACGGAAAA TGTTAGCAAA ACGGAAACCC CCTGCCATGG GTCAGGCCCC TCCCGCAACC 
AATGAGCAGA AACAACGGAA AAGCAAGACC AATGGAGCTG AAAATGAAAC GTTAACCTTA 
GCTGAATACC ATGAACAAGA AGAAATCTTC AAACTTAGAT TAGGTCATCT TAAGAAAGAG 
GAAGCAGAAA TCCAGGCAGA ACTGGAAAGG CTAGAAAGGG TTAGGAATCT ACACATCAGG 
GAATTAAAAA GGATACATAA TGAAGATAAT TCACAGTTTA AAGACCACCC AACACTAAAT 
GACAGATATT TGTTGTTACA TCTTTTGGGT AGAGGAGGTT TCAGTGAAGT TTACAAGGCG 
TTTGATCTAA CGGAGCAAAG ATATGTAGCT GTGAAAATTC ACCAGTTAAA TAAAAACTGG 
AGAGATGAGA AAAAGGAGAA TTACCACAAG CATGCGTGTA GGGAATACCG GATTCACAAA 
GAACTGGATC ACCCCAGAAT AGTGAAGCTG TATGATTACT TTTCACTGGA CACTGACTCG 
TTTTGTACAG TATTAGAATA CTGTGAAGGA AATGATCTGG ACTTCTACCT AAAGCAGCAC 
AAATTAATGT CGGAGAAAGA AGCCCGATCC ATTATTATGC AGATTGTGAA TGCTTTAAAG 
TACTTAAATG AAATAAAACC TCCCATCATA CACTATGACC TCAAACCAGG TAATATCCTT 
TTAGTAAATG GTACAGCGTG TGGAGAGATA AAAATTACAG ATTTTGGTCT TTCCAAGATC 
ATGGATGACG ATAGCTACAA TTCAGTGGAC GGCATGGAGC TAACGTCACA AGGTGCTGGT 
ACTTATTGGT ATTTGCCACC AGAGTGTTTT GTGGTTGGGA AAGAGCCACC AAAGATCTCA 
AATAAAGTCG ATGTCTGGTC AGTGGGTGTG ATCTTCTACC AGTGTCTGTA TGGGAGGAAG 
CCTTTTGGTC ATAACCAGTC CCAGCAAGAT ATTCTACAAG AGAATACTAT TCTTAAAGCT 
ACTGAAGTAC AGTTCCCGCC CAAGCCAGTA GTAACACCTG AAGCAAAGGC GTTTATCAGG 
CGATGTTTGG CCTATCGAAA GGAAGACCGC ATTGATGTGC AGCAGCTGGC CTGTGACCCC 
TACTTGTTGC CTCACATCCG AAAGTCAGTC TCCACAAGTA GTCCTGCAGG AGCTGCCATT 
GCATCAACCT CGGGGGCATC CAATAACAGT TCTTCGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452823.1
CDS	3..2144
Translation	MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSTAKEASEEQSALPTLMSVMLAKPRLDTEQLAPRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN

Target ORF information:

RefSeq Version	XM_017597334.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597334.1

ORF Insert Sequence:

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTTTGTGC 
AGTGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 
CGAAATCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG 
GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT 
GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA 
CCGCGTCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GTACTGCAAA GGAGGCCTCA 
GAAGAGCAGT CTGCCCTGCC AACCCTCATG TCAGTGATGT TAGCAAAACC TCGACTTGAC 
ACAGAGCAGT TAGCGCCAAG GGGAGCTGGC CTCTGCTTCA CTTTCGTCTC TGCTCAACAA 
AACAGCCCTT CGTCCACGGG GTCTGGCAAT ACAGAACATT CTTGCAGCTC CCAGAAACAG 
ATCTCCATCC AGCACAGGCA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA 
GAAAACAGTA AGAACTCTGA CTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA 
TCAAAACAAG AGAAGATGGC ATGCAGGGAC AAGAGCATGC AGGACCGATT GCGATTAGGT 
CACTTTACAA CTGTCCGGCA TGGAGCCTCT TTTACTGAAC AGTGGACAGA TGGTTATGCT 
TTCCAAAACC TCATCAAGCA ACAGGAAAGG ATAAATTCAC AGAGAGAAGA GATAGAAAGG 
CAACGGAAAA TGTTAGCAAA ACGGAAACCC CCTGCCATGG GTCAGGCCCC TCCCGCAACC 
AATGAGCAGA AACAACGGAA AAGCAAGACC AATGGAGCTG AAAATGAAAC GTTAACCTTA 
GCTGAATACC ATGAACAAGA AGAAATCTTC AAACTTAGAT TAGGTCATCT TAAGAAAGAG 
GAAGCAGAAA TCCAGGCAGA ACTGGAAAGG CTAGAAAGGG TTAGGAATCT ACACATCAGG 
GAATTAAAAA GGATACATAA TGAAGATAAT TCACAGTTTA AAGACCACCC AACACTAAAT 
GACAGATATT TGTTGTTACA TCTTTTGGGT AGAGGAGGTT TCAGTGAAGT TTACAAGGCG 
TTTGATCTAA CGGAGCAAAG ATATGTAGCT GTGAAAATTC ACCAGTTAAA TAAAAACTGG 
AGAGATGAGA AAAAGGAGAA TTACCACAAG CATGCGTGTA GGGAATACCG GATTCACAAA 
GAACTGGATC ACCCCAGAAT AGTGAAGCTG TATGATTACT TTTCACTGGA CACTGACTCG 
TTTTGTACAG TATTAGAATA CTGTGAAGGA AATGATCTGG ACTTCTACCT AAAGCAGCAC 
AAATTAATGT CGGAGAAAGA AGCCCGATCC ATTATTATGC AGATTGTGAA TGCTTTAAAG 
TACTTAAATG AAATAAAACC TCCCATCATA CACTATGACC TCAAACCAGG TAATATCCTT 
TTAGTAAATG GTACAGCGTG TGGAGAGATA AAAATTACAG ATTTTGGTCT TTCCAAGATC 
ATGGATGACG ATAGCTACAA TTCAGTGGAC GGCATGGAGC TAACGTCACA AGGTGCTGGT 
ACTTATTGGT ATTTGCCACC AGAGTGTTTT GTGGTTGGGA AAGAGCCACC AAAGATCTCA 
AATAAAGTCG ATGTCTGGTC AGTGGGTGTG ATCTTCTACC AGTGTCTGTA TGGGAGGAAG 
CCTTTTGGTC ATAACCAGTC CCAGCAAGAT ATTCTACAAG AGAATACTAT TCTTAAAGCT 
ACTGAAGTAC AGTTCCCGCC CAAGCCAGTA GTAACACCTG AAGCAAAGGC GTTTATCAGG 
CGATGTTTGG CCTATCGAAA GGAAGACCGC ATTGATGTGC AGCAGCTGGC CTGTGACCCC 
TACTTGTTGC CTCACATCCG AAAGTCAGTC TCCACAAGTA GTCCTGCAGG AGCTGCCATT 
GCATCAACCT CGGGGGCATC CAATAACAGT TCTTCGAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tlk2 ( XM_017597336.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452825.1 Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X6, mRNA.

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CloneID	ORa57368
Clone ID Related Accession (Same CDS sequence)	XM_017597336.1
Accession Version	XM_017597336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase tousled-like 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GCGTCGAGTA GAACAGCCTC TCTATGGTTT AGATGGCAGT 
ACTGCAAAGG AGGCCTCAGA AGAGCAGTCT GCCCTGCCAA CCCTCATGTC AGTGATGTTA 
GCAAAACCTC GACTTGACAC AGAGCAGTTA GCGCCAAGGG GAGCTGGCCT CTGCTTCACT 
TTCGTCTCTG CTCAACAAAA CAGCCCTTCG TCCACGGGGT CTGGCAATAC AGAACATTCT 
TGCAGCTCCC AGAAACAGAT CTCCATCCAG CACAGGCAGA CCCAGTCTGA CCTCACAATA 
GAAAAAATAT CTGCACTAGA AAACAGTAAG AACTCTGACT TAGAGAAGAA GGAAGGAAGA 
ATAGATGATT TATTAAGAGC CAACTGTGAT TTGAGACGAC AGATAGATGA ACAGCAAAAG 
ATGCTAGAGA AATACAAGGA ACGATTAAAT AGATGTGTCA CCATGAGCAA GAAGCTCCTT 
ATAGAAAAGT CAAAACAAGA GAAGATGGCA TGCAGGGACA AGAGCATGCA GGACCGATTG 
CGATTAGGTC ACTTTACAAC TGTCCGGCAT GGAGCCTCTT TTACTGAACA GTGGACAGAT 
GGTTATGCTT TCCAAAACCT CATCAAGCAA CAGGAAAGGA TAAATTCACA GAGAGAAGAG 
ATAGAAAGGC AACGGAAAAT GTTAGCAAAA CGGAAACCCC CTGCCATGGG TCAGGCCCCT 
CCCGCAACCA ATGAGCAGAA ACAACGGAAA AGCAAGACCA ATGGAGCTGA AAATGAAACG 
TTAACCTTAG CTGAATACCA TGAACAAGAA GAAATCTTCA AACTTAGATT AGGTCATCTT 
AAGAAAGAGG AAGCAGAAAT CCAGGCAGAA CTGGAAAGGC TAGAAAGGGT TAGGAATCTA 
CACATCAGGG AATTAAAAAG GATACATAAT GAAGATAATT CACAGTTTAA AGACCACCCA 
ACACTAAATG ACAGATATTT GTTGTTACAT CTTTTGGGTA GAGGAGGTTT CAGTGAAGTT 
TACAAGGCGT TTGATCTAAC GGAGCAAAGA TATGTAGCTG TGAAAATTCA CCAGTTAAAT 
AAAAACTGGA GAGATGAGAA AAAGGAGAAT TACCACAAGC ATGCGTGTAG GGAATACCGG 
ATTCACAAAG AACTGGATCA CCCCAGAATA GTGAAGCTGT ATGATTACTT TTCACTGGAC 
ACTGACTCGT TTTGTACAGT ATTAGAATAC TGTGAAGGAA ATGATCTGGA CTTCTACCTA 
AAGCAGCACA AATTAATGTC GGAGAAAGAA GCCCGATCCA TTATTATGCA GATTGTGAAT 
GCTTTAAAGT ACTTAAATGA AATAAAACCT CCCATCATAC ACTATGACCT CAAACCAGGT 
AATATCCTTT TAGTAAATGG TACAGCGTGT GGAGAGATAA AAATTACAGA TTTTGGTCTT 
TCCAAGATCA TGGATGACGA TAGCTACAAT TCAGTGGACG GCATGGAGCT AACGTCACAA 
GGTGCTGGTA CTTATTGGTA TTTGCCACCA GAGTGTTTTG TGGTTGGGAA AGAGCCACCA 
AAGATCTCAA ATAAAGTCGA TGTCTGGTCA GTGGGTGTGA TCTTCTACCA GTGTCTGTAT 
GGGAGGAAGC CTTTTGGTCA TAACCAGTCC CAGCAAGATA TTCTACAAGA GAATACTATT 
CTTAAAGCTA CTGAAGTACA GTTCCCGCCC AAGCCAGTAG TAACACCTGA AGCAAAGGCG 
TTTATCAGGC GATGTTTGGC CTATCGAAAG GAAGACCGCA TTGATGTGCA GCAGCTGGCC 
TGTGACCCCT ACTTGTTGCC TCACATCCGA AAGTCAGTCT CCACAAGTAG TCCTGCAGGA 
GCTGCCATTG CATCAACCTC GGGGGCATCC AATAACAGTT CTTCGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452825.1
CDS	483..2453
Translation	MMEELHSLDPRRQELLEARFTGVGVSKRRVEQPLYGLDGSTAKEASEEQSALPTLMSVMLAKPRLDTEQLAPRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN

Target ORF information:

RefSeq Version	XM_017597336.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tousled-like kinase 2 (Tlk2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597336.1

ORF Insert Sequence:

ATGATGGAAG AACTGCACAG CCTGGACCCA CGGAGGCAGG AGTTACTGGA GGCCAGGTTC 
ACTGGGGTTG GCGTAAGTAA GCGTCGAGTA GAACAGCCTC TCTATGGTTT AGATGGCAGT 
ACTGCAAAGG AGGCCTCAGA AGAGCAGTCT GCCCTGCCAA CCCTCATGTC AGTGATGTTA 
GCAAAACCTC GACTTGACAC AGAGCAGTTA GCGCCAAGGG GAGCTGGCCT CTGCTTCACT 
TTCGTCTCTG CTCAACAAAA CAGCCCTTCG TCCACGGGGT CTGGCAATAC AGAACATTCT 
TGCAGCTCCC AGAAACAGAT CTCCATCCAG CACAGGCAGA CCCAGTCTGA CCTCACAATA 
GAAAAAATAT CTGCACTAGA AAACAGTAAG AACTCTGACT TAGAGAAGAA GGAAGGAAGA 
ATAGATGATT TATTAAGAGC CAACTGTGAT TTGAGACGAC AGATAGATGA ACAGCAAAAG 
ATGCTAGAGA AATACAAGGA ACGATTAAAT AGATGTGTCA CCATGAGCAA GAAGCTCCTT 
ATAGAAAAGT CAAAACAAGA GAAGATGGCA TGCAGGGACA AGAGCATGCA GGACCGATTG 
CGATTAGGTC ACTTTACAAC TGTCCGGCAT GGAGCCTCTT TTACTGAACA GTGGACAGAT 
GGTTATGCTT TCCAAAACCT CATCAAGCAA CAGGAAAGGA TAAATTCACA GAGAGAAGAG 
ATAGAAAGGC AACGGAAAAT GTTAGCAAAA CGGAAACCCC CTGCCATGGG TCAGGCCCCT 
CCCGCAACCA ATGAGCAGAA ACAACGGAAA AGCAAGACCA ATGGAGCTGA AAATGAAACG 
TTAACCTTAG CTGAATACCA TGAACAAGAA GAAATCTTCA AACTTAGATT AGGTCATCTT 
AAGAAAGAGG AAGCAGAAAT CCAGGCAGAA CTGGAAAGGC TAGAAAGGGT TAGGAATCTA 
CACATCAGGG AATTAAAAAG GATACATAAT GAAGATAATT CACAGTTTAA AGACCACCCA 
ACACTAAATG ACAGATATTT GTTGTTACAT CTTTTGGGTA GAGGAGGTTT CAGTGAAGTT 
TACAAGGCGT TTGATCTAAC GGAGCAAAGA TATGTAGCTG TGAAAATTCA CCAGTTAAAT 
AAAAACTGGA GAGATGAGAA AAAGGAGAAT TACCACAAGC ATGCGTGTAG GGAATACCGG 
ATTCACAAAG AACTGGATCA CCCCAGAATA GTGAAGCTGT ATGATTACTT TTCACTGGAC 
ACTGACTCGT TTTGTACAGT ATTAGAATAC TGTGAAGGAA ATGATCTGGA CTTCTACCTA 
AAGCAGCACA AATTAATGTC GGAGAAAGAA GCCCGATCCA TTATTATGCA GATTGTGAAT 
GCTTTAAAGT ACTTAAATGA AATAAAACCT CCCATCATAC ACTATGACCT CAAACCAGGT 
AATATCCTTT TAGTAAATGG TACAGCGTGT GGAGAGATAA AAATTACAGA TTTTGGTCTT 
TCCAAGATCA TGGATGACGA TAGCTACAAT TCAGTGGACG GCATGGAGCT AACGTCACAA 
GGTGCTGGTA CTTATTGGTA TTTGCCACCA GAGTGTTTTG TGGTTGGGAA AGAGCCACCA 
AAGATCTCAA ATAAAGTCGA TGTCTGGTCA GTGGGTGTGA TCTTCTACCA GTGTCTGTAT 
GGGAGGAAGC CTTTTGGTCA TAACCAGTCC CAGCAAGATA TTCTACAAGA GAATACTATT 
CTTAAAGCTA CTGAAGTACA GTTCCCGCCC AAGCCAGTAG TAACACCTGA AGCAAAGGCG 
TTTATCAGGC GATGTTTGGC CTATCGAAAG GAAGACCGCA TTGATGTGCA GCAGCTGGCC 
TGTGACCCCT ACTTGTTGCC TCACATCCGA AAGTCAGTCT CCACAAGTAG TCCTGCAGGA 
GCTGCCATTG CATCAACCTC GGGGGCATCC AATAACAGTT CTTCGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Caenorhabditis elegans TLK-1 controls cytokinesis by localizing AIR-2/Aurora B to midzone microtubules.
Biochemical and biophysical research communications400(2)187-93(2010 Sep)
Yeh CH,Yang HJ,Lee IJ,Wu YC

Phosphorylation-mediated control of histone chaperone ASF1 levels by Tousled-like kinases.
PloS one4(12)e8328(2009 Dec)
Pilyugin M,Demmers J,Verrijzer CP,Karch F,Moshkin YM

Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint.
The EMBO journal22(7)1676-87(2003 Apr)
Groth A,Lukas J,Nigg EA,Sillj? HH,Wernstedt C,Bartek J,Hansen K

Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication.
The EMBO journal18(20)5691-702(1999 Oct)
Sillj? HH,Takahashi K,Tanaka K,Van Houwe G,Nigg EA

Nuclear localization of protein kinase U-alpha is regulated by 14-3-3.
The Journal of biological chemistry274(35)24865-72(1999 Aug)
Zhang S,Xing H,Muslin AJ

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Tlk2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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