TLK2 cDNA ORF clone, Bos taurus(cattle)

The following TLK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa30156 NM_001192827.1
Latest version!
Bos taurus tousled like kinase 2 (TLK2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa30156 XM_005220906.4
Latest version!
Bos taurus tousled like kinase 2 (TLK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa52917 XM_010816371.3
Latest version!
Bos taurus tousled like kinase 2 (TLK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa226452 XM_024980280.1
Latest version!
Bos taurus tousled like kinase 2 (TLK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa226453 XM_024980282.1
Latest version!
Bos taurus tousled like kinase 2 (TLK2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa52918 XM_024980279.1
Latest version!
Bos taurus tousled like kinase 2 (TLK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa52919 XM_024980281.1
Latest version!
Bos taurus tousled like kinase 2 (TLK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OBa52917 XM_010816371.2 Bos taurus tousled like kinase 2 (TLK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa30156 XM_005220906.3 Bos taurus tousled like kinase 2 (TLK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa30156
    Clone ID Related Accession (Same CDS sequence) XM_005220906.3 , XM_005220906.4 , NM_001192827.1
    Accession Version NM_001192827.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000019.1. On Jul 17, 2010 this sequence version replaced XM_614568.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5721695.3203.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT
    GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA
    CCGCGGCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GCGCTACAAA GGAGGTGTCA
    GAGGAGCAGT CTGCCCTGCC AACGCTTATG TCAGTGATGC TGGCAAAACC TCGGCTCGAC
    ACAGAGCAGC TGGCACAGAG GGGAGCTGGC CTCTGCTTCA CTTTTGTCTC AGCTCAGCAA
    AACAGTCCCT CATCTACGGG ATCTGGCAAC ACAGAGCATT CCTGCAGCTC CCAAAAACAG
    ATCTCCATCC AGCACAGACA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA
    GAAAACAGTA AGAATTCTGA TTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA
    GCCAACTGTG ATTTGAGACG GCAGATTGAT GAACAGCAAA AGATGTTAGA GAAATACAAA
    GAACGATTAA ATCGATGTGT GACGATGAGC AAGAAACTGC TTATAGAAAA GTCAAAACAA
    GAGAAGATGG CATGTAGAGA TAAAAGCATG CAAGATCGCT TGAGACTGGG CCACTTTACC
    ACAGTCCGAC ATGGGGCTTC GTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAGAAC
    CTTATTAAGC AACAGGAAAG GATAAATTCA CAGAGGGAAG AGATAGAAAG ACAACGAAAA
    ATGTTAGCGA AGCGGAAACC TCCTGCCATG GGACAGGCCC CTCCAGCAAC CAATGAGCAG
    AAACAGCGGA AAAGCAAGAC CAACGGAGCT GAGAATGAAA CGTTAACATT AGCAGAATAC
    CATGAACAAG AAGAAATCTT CAAACTCAGA TTAGGTCATC TTAAAAAGGA GGAAGCAGAG
    ATCCAGGCAG AGCTGGAAAG GCTAGAAAGG GTTAGAAATC TACATATCAG GGAACTAAAA
    AGGATACATA ATGAAGATAA TTCACAATTT AAAGATCATC CAACGCTAAA TGACAGATAT
    TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC ATTTGATCTA
    ACAGAACAAA GATACGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG
    AAAAAGGAGA ATTACCACAA GCATGCATGT AGGGAATACC GGATTCACAA AGAACTGGAT
    CATCCCAGAA TAGTTAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA
    GTACTAGAAT ACTGTGAGGG AAATGATCTG GACTTCTACC TGAAACAGCA CAAATTAATG
    TCGGAGAAAG AAGCCCGATC CATTATCATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT
    GAAATAAAAC CTCCTATCAT ACACTATGAC CTCAAACCAG GTAATATTCT TTTAGTAAAT
    GGTACAGCGT GTGGAGAGAT AAAAATCACA GATTTTGGTC TTTCTAAGAT AATGGATGAT
    GATAGCTACA ATTCAGTGGA TGGCATGGAG CTAACATCAC AAGGTGCTGG TACTTACTGG
    TATTTACCGC CAGAGTGTTT TGTGGTTGGG AAAGAACCAC CAAAGATCTC AAATAAAGTT
    GACGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTTT ACGGAAGGAA GCCTTTTGGC
    CATAATCAGT CCCAGCAAGA CATTCTACAA GAAAATACTA TCCTCAAAGC TACTGAAGTG
    CAGTTCCCGC CAAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATTCG ACGATGCTTG
    GCCTACCGAA AGGAGGACCG CATCGACGTC CAGCAGCTGG CCTGTGACCC CTACTTGCTT
    CCTCACATCC GAAAGTCGGT CTCCACGAGT AGCCCTGCTG GAGCTGCTAT TGCGTCAACC
    TCGGGGGCGT CCAATAACAG TTCTTCGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179756.1
    CDS144..2396
    Translation

    Target ORF information:

    RefSeq Version NM_001192827.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 2 (TLK2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192827.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT
    GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA
    CCGCGGCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GCGCTACAAA GGAGGTGTCA
    GAGGAGCAGT CTGCCCTGCC AACGCTTATG TCAGTGATGC TGGCAAAACC TCGGCTCGAC
    ACAGAGCAGC TGGCACAGAG GGGAGCTGGC CTCTGCTTCA CTTTTGTCTC AGCTCAGCAA
    AACAGTCCCT CATCTACGGG ATCTGGCAAC ACAGAGCATT CCTGCAGCTC CCAAAAACAG
    ATCTCCATCC AGCACAGACA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA
    GAAAACAGTA AGAATTCTGA TTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA
    GCCAACTGTG ATTTGAGACG GCAGATTGAT GAACAGCAAA AGATGTTAGA GAAATACAAA
    GAACGATTAA ATCGATGTGT GACGATGAGC AAGAAACTGC TTATAGAAAA GTCAAAACAA
    GAGAAGATGG CATGTAGAGA TAAAAGCATG CAAGATCGCT TGAGACTGGG CCACTTTACC
    ACAGTCCGAC ATGGGGCTTC GTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAGAAC
    CTTATTAAGC AACAGGAAAG GATAAATTCA CAGAGGGAAG AGATAGAAAG ACAACGAAAA
    ATGTTAGCGA AGCGGAAACC TCCTGCCATG GGACAGGCCC CTCCAGCAAC CAATGAGCAG
    AAACAGCGGA AAAGCAAGAC CAACGGAGCT GAGAATGAAA CGTTAACATT AGCAGAATAC
    CATGAACAAG AAGAAATCTT CAAACTCAGA TTAGGTCATC TTAAAAAGGA GGAAGCAGAG
    ATCCAGGCAG AGCTGGAAAG GCTAGAAAGG GTTAGAAATC TACATATCAG GGAACTAAAA
    AGGATACATA ATGAAGATAA TTCACAATTT AAAGATCATC CAACGCTAAA TGACAGATAT
    TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC ATTTGATCTA
    ACAGAACAAA GATACGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG
    AAAAAGGAGA ATTACCACAA GCATGCATGT AGGGAATACC GGATTCACAA AGAACTGGAT
    CATCCCAGAA TAGTTAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA
    GTACTAGAAT ACTGTGAGGG AAATGATCTG GACTTCTACC TGAAACAGCA CAAATTAATG
    TCGGAGAAAG AAGCCCGATC CATTATCATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT
    GAAATAAAAC CTCCTATCAT ACACTATGAC CTCAAACCAG GTAATATTCT TTTAGTAAAT
    GGTACAGCGT GTGGAGAGAT AAAAATCACA GATTTTGGTC TTTCTAAGAT AATGGATGAT
    GATAGCTACA ATTCAGTGGA TGGCATGGAG CTAACATCAC AAGGTGCTGG TACTTACTGG
    TATTTACCGC CAGAGTGTTT TGTGGTTGGG AAAGAACCAC CAAAGATCTC AAATAAAGTT
    GACGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTTT ACGGAAGGAA GCCTTTTGGC
    CATAATCAGT CCCAGCAAGA CATTCTACAA GAAAATACTA TCCTCAAAGC TACTGAAGTG
    CAGTTCCCGC CAAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATTCG ACGATGCTTG
    GCCTACCGAA AGGAGGACCG CATCGACGTC CAGCAGCTGG CCTGTGACCC CTACTTGCTT
    CCTCACATCC GAAAGTCGGT CTCCACGAGT AGCCCTGCTG GAGCTGCTAT TGCGTCAACC
    TCGGGGGCGT CCAATAACAG TTCTTCGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30156
    Clone ID Related Accession (Same CDS sequence) XM_005220906.3 , XM_005220906.4 , NM_001192827.1
    Accession Version XM_005220906.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT
    GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA
    CCGCGGCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GCGCTACAAA GGAGGTGTCA
    GAGGAGCAGT CTGCCCTGCC AACGCTTATG TCAGTGATGC TGGCAAAACC TCGGCTCGAC
    ACAGAGCAGC TGGCACAGAG GGGAGCTGGC CTCTGCTTCA CTTTTGTCTC AGCTCAGCAA
    AACAGTCCCT CATCTACGGG ATCTGGCAAC ACAGAGCATT CCTGCAGCTC CCAAAAACAG
    ATCTCCATCC AGCACAGACA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA
    GAAAACAGTA AGAATTCTGA TTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA
    GCCAACTGTG ATTTGAGACG GCAGATTGAT GAACAGCAAA AGATGTTAGA GAAATACAAA
    GAACGATTAA ATCGATGTGT GACGATGAGC AAGAAACTGC TTATAGAAAA GTCAAAACAA
    GAGAAGATGG CATGTAGAGA TAAAAGCATG CAAGATCGCT TGAGACTGGG CCACTTTACC
    ACAGTCCGAC ATGGGGCTTC GTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAGAAC
    CTTATTAAGC AACAGGAAAG GATAAATTCA CAGAGGGAAG AGATAGAAAG ACAACGAAAA
    ATGTTAGCGA AGCGGAAACC TCCTGCCATG GGACAGGCCC CTCCAGCAAC CAATGAGCAG
    AAACAGCGGA AAAGCAAGAC CAACGGAGCT GAGAATGAAA CGTTAACATT AGCAGAATAC
    CATGAACAAG AAGAAATCTT CAAACTCAGA TTAGGTCATC TTAAAAAGGA GGAAGCAGAG
    ATCCAGGCAG AGCTGGAAAG GCTAGAAAGG GTTAGAAATC TACATATCAG GGAACTAAAA
    AGGATACATA ATGAAGATAA TTCACAATTT AAAGATCATC CAACGCTAAA TGACAGATAT
    TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC ATTTGATCTA
    ACAGAACAAA GATACGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG
    AAAAAGGAGA ATTACCACAA GCATGCATGT AGGGAATACC GGATTCACAA AGAACTGGAT
    CATCCCAGAA TAGTTAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA
    GTACTAGAAT ACTGTGAGGG AAATGATCTG GACTTCTACC TGAAACAGCA CAAATTAATG
    TCGGAGAAAG AAGCCCGATC CATTATCATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT
    GAAATAAAAC CTCCTATCAT ACACTATGAC CTCAAACCAG GTAATATTCT TTTAGTAAAT
    GGTACAGCGT GTGGAGAGAT AAAAATCACA GATTTTGGTC TTTCTAAGAT AATGGATGAT
    GATAGCTACA ATTCAGTGGA TGGCATGGAG CTAACATCAC AAGGTGCTGG TACTTACTGG
    TATTTACCGC CAGAGTGTTT TGTGGTTGGG AAAGAACCAC CAAAGATCTC AAATAAAGTT
    GACGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTTT ACGGAAGGAA GCCTTTTGGC
    CATAATCAGT CCCAGCAAGA CATTCTACAA GAAAATACTA TCCTCAAAGC TACTGAAGTG
    CAGTTCCCGC CAAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATTCG ACGATGCTTG
    GCCTACCGAA AGGAGGACCG CATCGACGTC CAGCAGCTGG CCTGTGACCC CTACTTGCTT
    CCTCACATCC GAAAGTCGGT CTCCACGAGT AGCCCTGCTG GAGCTGCTAT TGCGTCAACC
    TCGGGGGCGT CCAATAACAG TTCTTCGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220963.1
    CDS503..2755
    Translation

    Target ORF information:

    RefSeq Version XM_005220906.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 2 (TLK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220906.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT
    GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA
    CCGCGGCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GCGCTACAAA GGAGGTGTCA
    GAGGAGCAGT CTGCCCTGCC AACGCTTATG TCAGTGATGC TGGCAAAACC TCGGCTCGAC
    ACAGAGCAGC TGGCACAGAG GGGAGCTGGC CTCTGCTTCA CTTTTGTCTC AGCTCAGCAA
    AACAGTCCCT CATCTACGGG ATCTGGCAAC ACAGAGCATT CCTGCAGCTC CCAAAAACAG
    ATCTCCATCC AGCACAGACA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA
    GAAAACAGTA AGAATTCTGA TTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA
    GCCAACTGTG ATTTGAGACG GCAGATTGAT GAACAGCAAA AGATGTTAGA GAAATACAAA
    GAACGATTAA ATCGATGTGT GACGATGAGC AAGAAACTGC TTATAGAAAA GTCAAAACAA
    GAGAAGATGG CATGTAGAGA TAAAAGCATG CAAGATCGCT TGAGACTGGG CCACTTTACC
    ACAGTCCGAC ATGGGGCTTC GTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAGAAC
    CTTATTAAGC AACAGGAAAG GATAAATTCA CAGAGGGAAG AGATAGAAAG ACAACGAAAA
    ATGTTAGCGA AGCGGAAACC TCCTGCCATG GGACAGGCCC CTCCAGCAAC CAATGAGCAG
    AAACAGCGGA AAAGCAAGAC CAACGGAGCT GAGAATGAAA CGTTAACATT AGCAGAATAC
    CATGAACAAG AAGAAATCTT CAAACTCAGA TTAGGTCATC TTAAAAAGGA GGAAGCAGAG
    ATCCAGGCAG AGCTGGAAAG GCTAGAAAGG GTTAGAAATC TACATATCAG GGAACTAAAA
    AGGATACATA ATGAAGATAA TTCACAATTT AAAGATCATC CAACGCTAAA TGACAGATAT
    TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC ATTTGATCTA
    ACAGAACAAA GATACGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG
    AAAAAGGAGA ATTACCACAA GCATGCATGT AGGGAATACC GGATTCACAA AGAACTGGAT
    CATCCCAGAA TAGTTAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA
    GTACTAGAAT ACTGTGAGGG AAATGATCTG GACTTCTACC TGAAACAGCA CAAATTAATG
    TCGGAGAAAG AAGCCCGATC CATTATCATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT
    GAAATAAAAC CTCCTATCAT ACACTATGAC CTCAAACCAG GTAATATTCT TTTAGTAAAT
    GGTACAGCGT GTGGAGAGAT AAAAATCACA GATTTTGGTC TTTCTAAGAT AATGGATGAT
    GATAGCTACA ATTCAGTGGA TGGCATGGAG CTAACATCAC AAGGTGCTGG TACTTACTGG
    TATTTACCGC CAGAGTGTTT TGTGGTTGGG AAAGAACCAC CAAAGATCTC AAATAAAGTT
    GACGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTTT ACGGAAGGAA GCCTTTTGGC
    CATAATCAGT CCCAGCAAGA CATTCTACAA GAAAATACTA TCCTCAAAGC TACTGAAGTG
    CAGTTCCCGC CAAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATTCG ACGATGCTTG
    GCCTACCGAA AGGAGGACCG CATCGACGTC CAGCAGCTGG CCTGTGACCC CTACTTGCTT
    CCTCACATCC GAAAGTCGGT CTCCACGAGT AGCCCTGCTG GAGCTGCTAT TGCGTCAACC
    TCGGGGGCGT CCAATAACAG TTCTTCGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30156
    Clone ID Related Accession (Same CDS sequence) XM_005220906.3 , XM_005220906.4 , NM_001192827.1
    Accession Version XM_005220906.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220906.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT
    GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA
    CCGCGGCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GCGCTACAAA GGAGGTGTCA
    GAGGAGCAGT CTGCCCTGCC AACGCTTATG TCAGTGATGC TGGCAAAACC TCGGCTCGAC
    ACAGAGCAGC TGGCACAGAG GGGAGCTGGC CTCTGCTTCA CTTTTGTCTC AGCTCAGCAA
    AACAGTCCCT CATCTACGGG ATCTGGCAAC ACAGAGCATT CCTGCAGCTC CCAAAAACAG
    ATCTCCATCC AGCACAGACA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA
    GAAAACAGTA AGAATTCTGA TTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA
    GCCAACTGTG ATTTGAGACG GCAGATTGAT GAACAGCAAA AGATGTTAGA GAAATACAAA
    GAACGATTAA ATCGATGTGT GACGATGAGC AAGAAACTGC TTATAGAAAA GTCAAAACAA
    GAGAAGATGG CATGTAGAGA TAAAAGCATG CAAGATCGCT TGAGACTGGG CCACTTTACC
    ACAGTCCGAC ATGGGGCTTC GTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAGAAC
    CTTATTAAGC AACAGGAAAG GATAAATTCA CAGAGGGAAG AGATAGAAAG ACAACGAAAA
    ATGTTAGCGA AGCGGAAACC TCCTGCCATG GGACAGGCCC CTCCAGCAAC CAATGAGCAG
    AAACAGCGGA AAAGCAAGAC CAACGGAGCT GAGAATGAAA CGTTAACATT AGCAGAATAC
    CATGAACAAG AAGAAATCTT CAAACTCAGA TTAGGTCATC TTAAAAAGGA GGAAGCAGAG
    ATCCAGGCAG AGCTGGAAAG GCTAGAAAGG GTTAGAAATC TACATATCAG GGAACTAAAA
    AGGATACATA ATGAAGATAA TTCACAATTT AAAGATCATC CAACGCTAAA TGACAGATAT
    TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC ATTTGATCTA
    ACAGAACAAA GATACGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG
    AAAAAGGAGA ATTACCACAA GCATGCATGT AGGGAATACC GGATTCACAA AGAACTGGAT
    CATCCCAGAA TAGTTAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA
    GTACTAGAAT ACTGTGAGGG AAATGATCTG GACTTCTACC TGAAACAGCA CAAATTAATG
    TCGGAGAAAG AAGCCCGATC CATTATCATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT
    GAAATAAAAC CTCCTATCAT ACACTATGAC CTCAAACCAG GTAATATTCT TTTAGTAAAT
    GGTACAGCGT GTGGAGAGAT AAAAATCACA GATTTTGGTC TTTCTAAGAT AATGGATGAT
    GATAGCTACA ATTCAGTGGA TGGCATGGAG CTAACATCAC AAGGTGCTGG TACTTACTGG
    TATTTACCGC CAGAGTGTTT TGTGGTTGGG AAAGAACCAC CAAAGATCTC AAATAAAGTT
    GACGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTTT ACGGAAGGAA GCCTTTTGGC
    CATAATCAGT CCCAGCAAGA CATTCTACAA GAAAATACTA TCCTCAAAGC TACTGAAGTG
    CAGTTCCCGC CAAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATTCG ACGATGCTTG
    GCCTACCGAA AGGAGGACCG CATCGACGTC CAGCAGCTGG CCTGTGACCC CTACTTGCTT
    CCTCACATCC GAAAGTCGGT CTCCACGAGT AGCCCTGCTG GAGCTGCTAT TGCGTCAACC
    TCGGGGGCGT CCAATAACAG TTCTTCGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220963.1
    CDS519..2771
    Translation

    Target ORF information:

    RefSeq Version XM_005220906.4
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 2 (TLK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220906.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT
    GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA
    CCGCGGCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GCGCTACAAA GGAGGTGTCA
    GAGGAGCAGT CTGCCCTGCC AACGCTTATG TCAGTGATGC TGGCAAAACC TCGGCTCGAC
    ACAGAGCAGC TGGCACAGAG GGGAGCTGGC CTCTGCTTCA CTTTTGTCTC AGCTCAGCAA
    AACAGTCCCT CATCTACGGG ATCTGGCAAC ACAGAGCATT CCTGCAGCTC CCAAAAACAG
    ATCTCCATCC AGCACAGACA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA
    GAAAACAGTA AGAATTCTGA TTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA
    GCCAACTGTG ATTTGAGACG GCAGATTGAT GAACAGCAAA AGATGTTAGA GAAATACAAA
    GAACGATTAA ATCGATGTGT GACGATGAGC AAGAAACTGC TTATAGAAAA GTCAAAACAA
    GAGAAGATGG CATGTAGAGA TAAAAGCATG CAAGATCGCT TGAGACTGGG CCACTTTACC
    ACAGTCCGAC ATGGGGCTTC GTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAGAAC
    CTTATTAAGC AACAGGAAAG GATAAATTCA CAGAGGGAAG AGATAGAAAG ACAACGAAAA
    ATGTTAGCGA AGCGGAAACC TCCTGCCATG GGACAGGCCC CTCCAGCAAC CAATGAGCAG
    AAACAGCGGA AAAGCAAGAC CAACGGAGCT GAGAATGAAA CGTTAACATT AGCAGAATAC
    CATGAACAAG AAGAAATCTT CAAACTCAGA TTAGGTCATC TTAAAAAGGA GGAAGCAGAG
    ATCCAGGCAG AGCTGGAAAG GCTAGAAAGG GTTAGAAATC TACATATCAG GGAACTAAAA
    AGGATACATA ATGAAGATAA TTCACAATTT AAAGATCATC CAACGCTAAA TGACAGATAT
    TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC ATTTGATCTA
    ACAGAACAAA GATACGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG
    AAAAAGGAGA ATTACCACAA GCATGCATGT AGGGAATACC GGATTCACAA AGAACTGGAT
    CATCCCAGAA TAGTTAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA
    GTACTAGAAT ACTGTGAGGG AAATGATCTG GACTTCTACC TGAAACAGCA CAAATTAATG
    TCGGAGAAAG AAGCCCGATC CATTATCATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT
    GAAATAAAAC CTCCTATCAT ACACTATGAC CTCAAACCAG GTAATATTCT TTTAGTAAAT
    GGTACAGCGT GTGGAGAGAT AAAAATCACA GATTTTGGTC TTTCTAAGAT AATGGATGAT
    GATAGCTACA ATTCAGTGGA TGGCATGGAG CTAACATCAC AAGGTGCTGG TACTTACTGG
    TATTTACCGC CAGAGTGTTT TGTGGTTGGG AAAGAACCAC CAAAGATCTC AAATAAAGTT
    GACGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTTT ACGGAAGGAA GCCTTTTGGC
    CATAATCAGT CCCAGCAAGA CATTCTACAA GAAAATACTA TCCTCAAAGC TACTGAAGTG
    CAGTTCCCGC CAAAGCCAGT AGTAACACCT GAAGCAAAGG CGTTTATTCG ACGATGCTTG
    GCCTACCGAA AGGAGGACCG CATCGACGTC CAGCAGCTGG CCTGTGACCC CTACTTGCTT
    CCTCACATCC GAAAGTCGGT CTCCACGAGT AGCCCTGCTG GAGCTGCTAT TGCGTCAACC
    TCGGGGGCGT CCAATAACAG TTCTTCGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52917
    Clone ID Related Accession (Same CDS sequence) XM_010816371.2 , XM_010816371.3
    Accession Version XM_010816371.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGCGG CGAGTAGAAC AGCCTCTCTA TGGTTTAGAT
    GGCAGCGCTA CAAAGGAGGT GTCAGAGGAG CAGTCTGCCC TGCCAACGCT TATGTCAGTG
    ATGCTGGCAA AACCTCGGCT CGACACAGAG CAGCTGGCAC AGAGGGGAGC TGGCCTCTGC
    TTCACTTTTG TCTCAGCTCA GCAAAACAGT CCCTCATCTA CGGGATCTGG CAACACAGAG
    CATTCCTGCA GCTCCCAAAA ACAGATCTCC ATCCAGCACA GACAGACCCA GTCTGACCTC
    ACAATAGAAA AAATATCTGC ACTAGAAAAC AGTAAGAATT CTGATTTAGA GAAGAAGGAA
    GGAAGAATAG ATGATTTATT AAGAGCCAAC TGTGATTTGA GACGGCAGAT TGATGAACAG
    CAAAAGATGT TAGAGAAATA CAAAGAACGA TTAAATCGAT GTGTGACGAT GAGCAAGAAA
    CTGCTTATAG AAAAGTCAAA ACAAGAGAAG ATGGCATGTA GAGATAAAAG CATGCAAGAT
    CGCTTGAGAC TGGGCCACTT TACCACAGTC CGACATGGGG CTTCGTTTAC TGAACAGTGG
    ACAGATGGTT ATGCTTTCCA GAACCTTATT AAGCAACAGG AAAGGATAAA TTCACAGAGG
    GAAGAGATAG AAAGACAACG AAAAATGTTA GCGAAGCGGA AACCTCCTGC CATGGGACAG
    GCCCCTCCAG CAACCAATGA GCAGAAACAG CGGAAAAGCA AGACCAACGG AGCTGAGAAT
    GAAACGTTAA CATTAGCAGA ATACCATGAA CAAGAAGAAA TCTTCAAACT CAGATTAGGT
    CATCTTAAAA AGGAGGAAGC AGAGATCCAG GCAGAGCTGG AAAGGCTAGA AAGGGTTAGA
    AATCTACATA TCAGGGAACT AAAAAGGATA CATAATGAAG ATAATTCACA ATTTAAAGAT
    CATCCAACGC TAAATGACAG ATATTTGTTG TTACATCTTT TGGGTAGAGG AGGTTTCAGT
    GAAGTTTACA AGGCATTTGA TCTAACAGAA CAAAGATACG TAGCTGTGAA AATTCACCAG
    TTAAATAAAA ACTGGAGAGA TGAGAAAAAG GAGAATTACC ACAAGCATGC ATGTAGGGAA
    TACCGGATTC ACAAAGAACT GGATCATCCC AGAATAGTTA AGCTGTATGA TTACTTTTCA
    CTGGACACTG ACTCGTTTTG TACAGTACTA GAATACTGTG AGGGAAATGA TCTGGACTTC
    TACCTGAAAC AGCACAAATT AATGTCGGAG AAAGAAGCCC GATCCATTAT CATGCAGATT
    GTGAATGCTT TAAAGTACTT AAATGAAATA AAACCTCCTA TCATACACTA TGACCTCAAA
    CCAGGTAATA TTCTTTTAGT AAATGGTACA GCGTGTGGAG AGATAAAAAT CACAGATTTT
    GGTCTTTCTA AGATAATGGA TGATGATAGC TACAATTCAG TGGATGGCAT GGAGCTAACA
    TCACAAGGTG CTGGTACTTA CTGGTATTTA CCGCCAGAGT GTTTTGTGGT TGGGAAAGAA
    CCACCAAAGA TCTCAAATAA AGTTGACGTC TGGTCAGTGG GTGTGATCTT CTACCAGTGT
    CTTTACGGAA GGAAGCCTTT TGGCCATAAT CAGTCCCAGC AAGACATTCT ACAAGAAAAT
    ACTATCCTCA AAGCTACTGA AGTGCAGTTC CCGCCAAAGC CAGTAGTAAC ACCTGAAGCA
    AAGGCGTTTA TTCGACGATG CTTGGCCTAC CGAAAGGAGG ACCGCATCGA CGTCCAGCAG
    CTGGCCTGTG ACCCCTACTT GCTTCCTCAC ATCCGAAAGT CGGTCTCCAC GAGTAGCCCT
    GCTGGAGCTG CTATTGCGTC AACCTCGGGG GCGTCCAATA ACAGTTCTTC GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814673.1
    CDS502..2658
    Translation

    Target ORF information:

    RefSeq Version XM_010816371.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 2 (TLK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816371.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGCGG CGAGTAGAAC AGCCTCTCTA TGGTTTAGAT
    GGCAGCGCTA CAAAGGAGGT GTCAGAGGAG CAGTCTGCCC TGCCAACGCT TATGTCAGTG
    ATGCTGGCAA AACCTCGGCT CGACACAGAG CAGCTGGCAC AGAGGGGAGC TGGCCTCTGC
    TTCACTTTTG TCTCAGCTCA GCAAAACAGT CCCTCATCTA CGGGATCTGG CAACACAGAG
    CATTCCTGCA GCTCCCAAAA ACAGATCTCC ATCCAGCACA GACAGACCCA GTCTGACCTC
    ACAATAGAAA AAATATCTGC ACTAGAAAAC AGTAAGAATT CTGATTTAGA GAAGAAGGAA
    GGAAGAATAG ATGATTTATT AAGAGCCAAC TGTGATTTGA GACGGCAGAT TGATGAACAG
    CAAAAGATGT TAGAGAAATA CAAAGAACGA TTAAATCGAT GTGTGACGAT GAGCAAGAAA
    CTGCTTATAG AAAAGTCAAA ACAAGAGAAG ATGGCATGTA GAGATAAAAG CATGCAAGAT
    CGCTTGAGAC TGGGCCACTT TACCACAGTC CGACATGGGG CTTCGTTTAC TGAACAGTGG
    ACAGATGGTT ATGCTTTCCA GAACCTTATT AAGCAACAGG AAAGGATAAA TTCACAGAGG
    GAAGAGATAG AAAGACAACG AAAAATGTTA GCGAAGCGGA AACCTCCTGC CATGGGACAG
    GCCCCTCCAG CAACCAATGA GCAGAAACAG CGGAAAAGCA AGACCAACGG AGCTGAGAAT
    GAAACGTTAA CATTAGCAGA ATACCATGAA CAAGAAGAAA TCTTCAAACT CAGATTAGGT
    CATCTTAAAA AGGAGGAAGC AGAGATCCAG GCAGAGCTGG AAAGGCTAGA AAGGGTTAGA
    AATCTACATA TCAGGGAACT AAAAAGGATA CATAATGAAG ATAATTCACA ATTTAAAGAT
    CATCCAACGC TAAATGACAG ATATTTGTTG TTACATCTTT TGGGTAGAGG AGGTTTCAGT
    GAAGTTTACA AGGCATTTGA TCTAACAGAA CAAAGATACG TAGCTGTGAA AATTCACCAG
    TTAAATAAAA ACTGGAGAGA TGAGAAAAAG GAGAATTACC ACAAGCATGC ATGTAGGGAA
    TACCGGATTC ACAAAGAACT GGATCATCCC AGAATAGTTA AGCTGTATGA TTACTTTTCA
    CTGGACACTG ACTCGTTTTG TACAGTACTA GAATACTGTG AGGGAAATGA TCTGGACTTC
    TACCTGAAAC AGCACAAATT AATGTCGGAG AAAGAAGCCC GATCCATTAT CATGCAGATT
    GTGAATGCTT TAAAGTACTT AAATGAAATA AAACCTCCTA TCATACACTA TGACCTCAAA
    CCAGGTAATA TTCTTTTAGT AAATGGTACA GCGTGTGGAG AGATAAAAAT CACAGATTTT
    GGTCTTTCTA AGATAATGGA TGATGATAGC TACAATTCAG TGGATGGCAT GGAGCTAACA
    TCACAAGGTG CTGGTACTTA CTGGTATTTA CCGCCAGAGT GTTTTGTGGT TGGGAAAGAA
    CCACCAAAGA TCTCAAATAA AGTTGACGTC TGGTCAGTGG GTGTGATCTT CTACCAGTGT
    CTTTACGGAA GGAAGCCTTT TGGCCATAAT CAGTCCCAGC AAGACATTCT ACAAGAAAAT
    ACTATCCTCA AAGCTACTGA AGTGCAGTTC CCGCCAAAGC CAGTAGTAAC ACCTGAAGCA
    AAGGCGTTTA TTCGACGATG CTTGGCCTAC CGAAAGGAGG ACCGCATCGA CGTCCAGCAG
    CTGGCCTGTG ACCCCTACTT GCTTCCTCAC ATCCGAAAGT CGGTCTCCAC GAGTAGCCCT
    GCTGGAGCTG CTATTGCGTC AACCTCGGGG GCGTCCAATA ACAGTTCTTC GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52917
    Clone ID Related Accession (Same CDS sequence) XM_010816371.2 , XM_010816371.3
    Accession Version XM_010816371.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816371.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGCGG CGAGTAGAAC AGCCTCTCTA TGGTTTAGAT
    GGCAGCGCTA CAAAGGAGGT GTCAGAGGAG CAGTCTGCCC TGCCAACGCT TATGTCAGTG
    ATGCTGGCAA AACCTCGGCT CGACACAGAG CAGCTGGCAC AGAGGGGAGC TGGCCTCTGC
    TTCACTTTTG TCTCAGCTCA GCAAAACAGT CCCTCATCTA CGGGATCTGG CAACACAGAG
    CATTCCTGCA GCTCCCAAAA ACAGATCTCC ATCCAGCACA GACAGACCCA GTCTGACCTC
    ACAATAGAAA AAATATCTGC ACTAGAAAAC AGTAAGAATT CTGATTTAGA GAAGAAGGAA
    GGAAGAATAG ATGATTTATT AAGAGCCAAC TGTGATTTGA GACGGCAGAT TGATGAACAG
    CAAAAGATGT TAGAGAAATA CAAAGAACGA TTAAATCGAT GTGTGACGAT GAGCAAGAAA
    CTGCTTATAG AAAAGTCAAA ACAAGAGAAG ATGGCATGTA GAGATAAAAG CATGCAAGAT
    CGCTTGAGAC TGGGCCACTT TACCACAGTC CGACATGGGG CTTCGTTTAC TGAACAGTGG
    ACAGATGGTT ATGCTTTCCA GAACCTTATT AAGCAACAGG AAAGGATAAA TTCACAGAGG
    GAAGAGATAG AAAGACAACG AAAAATGTTA GCGAAGCGGA AACCTCCTGC CATGGGACAG
    GCCCCTCCAG CAACCAATGA GCAGAAACAG CGGAAAAGCA AGACCAACGG AGCTGAGAAT
    GAAACGTTAA CATTAGCAGA ATACCATGAA CAAGAAGAAA TCTTCAAACT CAGATTAGGT
    CATCTTAAAA AGGAGGAAGC AGAGATCCAG GCAGAGCTGG AAAGGCTAGA AAGGGTTAGA
    AATCTACATA TCAGGGAACT AAAAAGGATA CATAATGAAG ATAATTCACA ATTTAAAGAT
    CATCCAACGC TAAATGACAG ATATTTGTTG TTACATCTTT TGGGTAGAGG AGGTTTCAGT
    GAAGTTTACA AGGCATTTGA TCTAACAGAA CAAAGATACG TAGCTGTGAA AATTCACCAG
    TTAAATAAAA ACTGGAGAGA TGAGAAAAAG GAGAATTACC ACAAGCATGC ATGTAGGGAA
    TACCGGATTC ACAAAGAACT GGATCATCCC AGAATAGTTA AGCTGTATGA TTACTTTTCA
    CTGGACACTG ACTCGTTTTG TACAGTACTA GAATACTGTG AGGGAAATGA TCTGGACTTC
    TACCTGAAAC AGCACAAATT AATGTCGGAG AAAGAAGCCC GATCCATTAT CATGCAGATT
    GTGAATGCTT TAAAGTACTT AAATGAAATA AAACCTCCTA TCATACACTA TGACCTCAAA
    CCAGGTAATA TTCTTTTAGT AAATGGTACA GCGTGTGGAG AGATAAAAAT CACAGATTTT
    GGTCTTTCTA AGATAATGGA TGATGATAGC TACAATTCAG TGGATGGCAT GGAGCTAACA
    TCACAAGGTG CTGGTACTTA CTGGTATTTA CCGCCAGAGT GTTTTGTGGT TGGGAAAGAA
    CCACCAAAGA TCTCAAATAA AGTTGACGTC TGGTCAGTGG GTGTGATCTT CTACCAGTGT
    CTTTACGGAA GGAAGCCTTT TGGCCATAAT CAGTCCCAGC AAGACATTCT ACAAGAAAAT
    ACTATCCTCA AAGCTACTGA AGTGCAGTTC CCGCCAAAGC CAGTAGTAAC ACCTGAAGCA
    AAGGCGTTTA TTCGACGATG CTTGGCCTAC CGAAAGGAGG ACCGCATCGA CGTCCAGCAG
    CTGGCCTGTG ACCCCTACTT GCTTCCTCAC ATCCGAAAGT CGGTCTCCAC GAGTAGCCCT
    GCTGGAGCTG CTATTGCGTC AACCTCGGGG GCGTCCAATA ACAGTTCTTC GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814673.1
    CDS519..2675
    Translation

    Target ORF information:

    RefSeq Version XM_010816371.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 2 (TLK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816371.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGCGG CGAGTAGAAC AGCCTCTCTA TGGTTTAGAT
    GGCAGCGCTA CAAAGGAGGT GTCAGAGGAG CAGTCTGCCC TGCCAACGCT TATGTCAGTG
    ATGCTGGCAA AACCTCGGCT CGACACAGAG CAGCTGGCAC AGAGGGGAGC TGGCCTCTGC
    TTCACTTTTG TCTCAGCTCA GCAAAACAGT CCCTCATCTA CGGGATCTGG CAACACAGAG
    CATTCCTGCA GCTCCCAAAA ACAGATCTCC ATCCAGCACA GACAGACCCA GTCTGACCTC
    ACAATAGAAA AAATATCTGC ACTAGAAAAC AGTAAGAATT CTGATTTAGA GAAGAAGGAA
    GGAAGAATAG ATGATTTATT AAGAGCCAAC TGTGATTTGA GACGGCAGAT TGATGAACAG
    CAAAAGATGT TAGAGAAATA CAAAGAACGA TTAAATCGAT GTGTGACGAT GAGCAAGAAA
    CTGCTTATAG AAAAGTCAAA ACAAGAGAAG ATGGCATGTA GAGATAAAAG CATGCAAGAT
    CGCTTGAGAC TGGGCCACTT TACCACAGTC CGACATGGGG CTTCGTTTAC TGAACAGTGG
    ACAGATGGTT ATGCTTTCCA GAACCTTATT AAGCAACAGG AAAGGATAAA TTCACAGAGG
    GAAGAGATAG AAAGACAACG AAAAATGTTA GCGAAGCGGA AACCTCCTGC CATGGGACAG
    GCCCCTCCAG CAACCAATGA GCAGAAACAG CGGAAAAGCA AGACCAACGG AGCTGAGAAT
    GAAACGTTAA CATTAGCAGA ATACCATGAA CAAGAAGAAA TCTTCAAACT CAGATTAGGT
    CATCTTAAAA AGGAGGAAGC AGAGATCCAG GCAGAGCTGG AAAGGCTAGA AAGGGTTAGA
    AATCTACATA TCAGGGAACT AAAAAGGATA CATAATGAAG ATAATTCACA ATTTAAAGAT
    CATCCAACGC TAAATGACAG ATATTTGTTG TTACATCTTT TGGGTAGAGG AGGTTTCAGT
    GAAGTTTACA AGGCATTTGA TCTAACAGAA CAAAGATACG TAGCTGTGAA AATTCACCAG
    TTAAATAAAA ACTGGAGAGA TGAGAAAAAG GAGAATTACC ACAAGCATGC ATGTAGGGAA
    TACCGGATTC ACAAAGAACT GGATCATCCC AGAATAGTTA AGCTGTATGA TTACTTTTCA
    CTGGACACTG ACTCGTTTTG TACAGTACTA GAATACTGTG AGGGAAATGA TCTGGACTTC
    TACCTGAAAC AGCACAAATT AATGTCGGAG AAAGAAGCCC GATCCATTAT CATGCAGATT
    GTGAATGCTT TAAAGTACTT AAATGAAATA AAACCTCCTA TCATACACTA TGACCTCAAA
    CCAGGTAATA TTCTTTTAGT AAATGGTACA GCGTGTGGAG AGATAAAAAT CACAGATTTT
    GGTCTTTCTA AGATAATGGA TGATGATAGC TACAATTCAG TGGATGGCAT GGAGCTAACA
    TCACAAGGTG CTGGTACTTA CTGGTATTTA CCGCCAGAGT GTTTTGTGGT TGGGAAAGAA
    CCACCAAAGA TCTCAAATAA AGTTGACGTC TGGTCAGTGG GTGTGATCTT CTACCAGTGT
    CTTTACGGAA GGAAGCCTTT TGGCCATAAT CAGTCCCAGC AAGACATTCT ACAAGAAAAT
    ACTATCCTCA AAGCTACTGA AGTGCAGTTC CCGCCAAAGC CAGTAGTAAC ACCTGAAGCA
    AAGGCGTTTA TTCGACGATG CTTGGCCTAC CGAAAGGAGG ACCGCATCGA CGTCCAGCAG
    CTGGCCTGTG ACCCCTACTT GCTTCCTCAC ATCCGAAAGT CGGTCTCCAC GAGTAGCCCT
    GCTGGAGCTG CTATTGCGTC AACCTCGGGG GCGTCCAATA ACAGTTCTTC GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226452
    Clone ID Related Accession (Same CDS sequence) XM_024980280.1
    Accession Version XM_024980280.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT
    GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA
    CCGCGGCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GCGCTACAAA GGAGGTGTCA
    GAGGAGCAGT CTGCCCTGCC AACGCTTATG TCAGTGATGC TGGCAAAACC TCGGCTCGAC
    ACAGAGCAGC TGGCACAGAG GGGAGCTGGC CTCTGCTTCA CTTTTGTCTC AGCTCAGCAA
    AACAGTCCCT CATCTACGGG ATCTGGCAAC ACAGAGCATT CCTGCAGCTC CCAAAAACAG
    ATCTCCATCC AGCACAGACA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA
    GAAAACAGTA AGAATTCTGA TTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA
    GCCAACTGTG ATTTGAGACG GCAGATTGAT GAACAGCAAA AGATGTTAGA GAAATACAAA
    GAACGATTAA ATCGATGTGT GACGATGAGC AAGAAACTGC TTATAGAAAA GTCAAAACAA
    GAGAAGATGG CATGTAGAGA TAAAAGCATG CAAGATCGCT TGAGACTGGG CCACTTTACC
    ACAGTCCGAC ATGGGGCTTC GTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAGAAC
    CTTATTAAGC AACAGGAAAG GATAAATTCA CAGAGGGAAG AGATAGAAAG ACAACGAAAA
    ATGTTAGCGA AGCGGAAACC TCCTGCCATG GGACAGGCCC CTCCAGCAAC CAATGAGCAG
    AAACAGCGGA AAAGCAAGAC CAACGGAGCT GAGAATGAAA CGTTAACATT AGCAGAATAC
    CATGAACAAG AAGAAATCTT CAAACTCAGA TTAGGTCATC TTAAAAAGGA GGAAGCAGAG
    ATCCAGGCAG AGCTGGAAAG GCTAGAAAGG GTTAGAAATC TACATATCAG GGAACTAAAA
    AGGATACATA ATGAAGATAA TTCACAATTT AAAGATCATC CAACGCTAAA TGACAGATAT
    TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC ATTTGATCTA
    ACAGAACAAA GATACGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG
    AAAAAGGAGA ATTACCACAA GCATGCATGT AGGGAATACC GGATTCACAA AGAACTGGAT
    CATCCCAGAA TAGTTAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA
    GTACTAGAAT ACTGTGAGGG AAATGATCTG GACTTCTACC TGAAACAGCA CAAATTAATG
    TCGGAGAAAG AAGCCCGATC CATTATCATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT
    GAAATAAAAC CTCCTATCAT ACACTATGAC CTCAAACCAG GTAATATTCT TTTAGTAAAT
    GGTACAGCGT GTGGAGAGAT AAAAATCACA GATTTTGGTC TTTCTAAGAT AATGGATGAT
    GATAGCTACA ATTCAGTGGA TGGCATGGAG CTAACATCAC AAGGTGCTGG TACTTACTGG
    TATTTACCGC CAGAGTGTTT TGTGGTTGGG AAAGAACCAC CAAAGATCTC AAATAAAGTT
    GACGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTTT ACGGAAGGAA GCCTTTTGGC
    CATAATCAGT CCCAGCAAGA CATTCTACAA GAAAATACTA TCCTCAAAGC TACTGAAGTG
    CAGTTCCCGC CAAAGCCAGT AGTAACACCT GAAGCAAAGC CTACCCTGGG AGCTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836048.1
    CDS519..2618
    Translation

    Target ORF information:

    RefSeq Version XM_024980280.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 2 (TLK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980280.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT
    GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA
    CCGCGGCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GCGCTACAAA GGAGGTGTCA
    GAGGAGCAGT CTGCCCTGCC AACGCTTATG TCAGTGATGC TGGCAAAACC TCGGCTCGAC
    ACAGAGCAGC TGGCACAGAG GGGAGCTGGC CTCTGCTTCA CTTTTGTCTC AGCTCAGCAA
    AACAGTCCCT CATCTACGGG ATCTGGCAAC ACAGAGCATT CCTGCAGCTC CCAAAAACAG
    ATCTCCATCC AGCACAGACA GACCCAGTCT GACCTCACAA TAGAAAAAAT ATCTGCACTA
    GAAAACAGTA AGAATTCTGA TTTAGAGAAG AAGGAAGGAA GAATAGATGA TTTATTAAGA
    GCCAACTGTG ATTTGAGACG GCAGATTGAT GAACAGCAAA AGATGTTAGA GAAATACAAA
    GAACGATTAA ATCGATGTGT GACGATGAGC AAGAAACTGC TTATAGAAAA GTCAAAACAA
    GAGAAGATGG CATGTAGAGA TAAAAGCATG CAAGATCGCT TGAGACTGGG CCACTTTACC
    ACAGTCCGAC ATGGGGCTTC GTTTACTGAA CAGTGGACAG ATGGTTATGC TTTCCAGAAC
    CTTATTAAGC AACAGGAAAG GATAAATTCA CAGAGGGAAG AGATAGAAAG ACAACGAAAA
    ATGTTAGCGA AGCGGAAACC TCCTGCCATG GGACAGGCCC CTCCAGCAAC CAATGAGCAG
    AAACAGCGGA AAAGCAAGAC CAACGGAGCT GAGAATGAAA CGTTAACATT AGCAGAATAC
    CATGAACAAG AAGAAATCTT CAAACTCAGA TTAGGTCATC TTAAAAAGGA GGAAGCAGAG
    ATCCAGGCAG AGCTGGAAAG GCTAGAAAGG GTTAGAAATC TACATATCAG GGAACTAAAA
    AGGATACATA ATGAAGATAA TTCACAATTT AAAGATCATC CAACGCTAAA TGACAGATAT
    TTGTTGTTAC ATCTTTTGGG TAGAGGAGGT TTCAGTGAAG TTTACAAGGC ATTTGATCTA
    ACAGAACAAA GATACGTAGC TGTGAAAATT CACCAGTTAA ATAAAAACTG GAGAGATGAG
    AAAAAGGAGA ATTACCACAA GCATGCATGT AGGGAATACC GGATTCACAA AGAACTGGAT
    CATCCCAGAA TAGTTAAGCT GTATGATTAC TTTTCACTGG ACACTGACTC GTTTTGTACA
    GTACTAGAAT ACTGTGAGGG AAATGATCTG GACTTCTACC TGAAACAGCA CAAATTAATG
    TCGGAGAAAG AAGCCCGATC CATTATCATG CAGATTGTGA ATGCTTTAAA GTACTTAAAT
    GAAATAAAAC CTCCTATCAT ACACTATGAC CTCAAACCAG GTAATATTCT TTTAGTAAAT
    GGTACAGCGT GTGGAGAGAT AAAAATCACA GATTTTGGTC TTTCTAAGAT AATGGATGAT
    GATAGCTACA ATTCAGTGGA TGGCATGGAG CTAACATCAC AAGGTGCTGG TACTTACTGG
    TATTTACCGC CAGAGTGTTT TGTGGTTGGG AAAGAACCAC CAAAGATCTC AAATAAAGTT
    GACGTCTGGT CAGTGGGTGT GATCTTCTAC CAGTGTCTTT ACGGAAGGAA GCCTTTTGGC
    CATAATCAGT CCCAGCAAGA CATTCTACAA GAAAATACTA TCCTCAAAGC TACTGAAGTG
    CAGTTCCCGC CAAAGCCAGT AGTAACACCT GAAGCAAAGC CTACCCTGGG AGCTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226453
    Clone ID Related Accession (Same CDS sequence) XM_024980282.1
    Accession Version XM_024980282.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GCGGCGAGTA GAACAGCCTC TCTATGGTTT AGATGGCAGC
    GCTACAAAGG AGGTGTCAGA GGAGCAGTCT GCCCTGCCAA CGCTTATGTC AGTGATGCTG
    GCAAAACCTC GGCTCGACAC AGAGCAGCTG GCACAGAGGG GAGCTGGCCT CTGCTTCACT
    TTTGTCTCAG CTCAGCAAAA CAGTCCCTCA TCTACGGGAT CTGGCAACAC AGAGCATTCC
    TGCAGCTCCC AAAAACAGAT CTCCATCCAG CACAGACAGA CCCAGTCTGA CCTCACAATA
    GAAAAAATAT CTGCACTAGA AAACAGTAAG AATTCTGATT TAGAGAAGAA GGAAGGAAGA
    ATAGATGATT TATTAAGAGC CAACTGTGAT TTGAGACGGC AGATTGATGA ACAGCAAAAG
    ATGTTAGAGA AATACAAAGA ACGATTAAAT CGATGTGTGA CGATGAGCAA GAAACTGCTT
    ATAGAAAAGT CAAAACAAGA GAAGATGGCA TGTAGAGATA AAAGCATGCA AGATCGCTTG
    AGACTGGGCC ACTTTACCAC AGTCCGACAT GGGGCTTCGT TTACTGAACA GTGGACAGAT
    GGTTATGCTT TCCAGAACCT TATTAAGCAA CAGGAAAGGA TAAATTCACA GAGGGAAGAG
    ATAGAAAGAC AACGAAAAAT GTTAGCGAAG CGGAAACCTC CTGCCATGGG ACAGGCCCCT
    CCAGCAACCA ATGAGCAGAA ACAGCGGAAA AGCAAGACCA ACGGAGCTGA GAATGAAACG
    TTAACATTAG CAGAATACCA TGAACAAGAA GAAATCTTCA AACTCAGATT AGGTCATCTT
    AAAAAGGAGG AAGCAGAGAT CCAGGCAGAG CTGGAAAGGC TAGAAAGGGT TAGAAATCTA
    CATATCAGGG AACTAAAAAG GATACATAAT GAAGATAATT CACAATTTAA AGATCATCCA
    ACGCTAAATG ACAGATATTT GTTGTTACAT CTTTTGGGTA GAGGAGGTTT CAGTGAAGTT
    TACAAGGCAT TTGATCTAAC AGAACAAAGA TACGTAGCTG TGAAAATTCA CCAGTTAAAT
    AAAAACTGGA GAGATGAGAA AAAGGAGAAT TACCACAAGC ATGCATGTAG GGAATACCGG
    ATTCACAAAG AACTGGATCA TCCCAGAATA GTTAAGCTGT ATGATTACTT TTCACTGGAC
    ACTGACTCGT TTTGTACAGT ACTAGAATAC TGTGAGGGAA ATGATCTGGA CTTCTACCTG
    AAACAGCACA AATTAATGTC GGAGAAAGAA GCCCGATCCA TTATCATGCA GATTGTGAAT
    GCTTTAAAGT ACTTAAATGA AATAAAACCT CCTATCATAC ACTATGACCT CAAACCAGGT
    AATATTCTTT TAGTAAATGG TACAGCGTGT GGAGAGATAA AAATCACAGA TTTTGGTCTT
    TCTAAGATAA TGGATGATGA TAGCTACAAT TCAGTGGATG GCATGGAGCT AACATCACAA
    GGTGCTGGTA CTTACTGGTA TTTACCGCCA GAGTGTTTTG TGGTTGGGAA AGAACCACCA
    AAGATCTCAA ATAAAGTTGA CGTCTGGTCA GTGGGTGTGA TCTTCTACCA GTGTCTTTAC
    GGAAGGAAGC CTTTTGGCCA TAATCAGTCC CAGCAAGACA TTCTACAAGA AAATACTATC
    CTCAAAGCTA CTGAAGTGCA GTTCCCGCCA AAGCCAGTAG TAACACCTGA AGCAAAGGCG
    TTTATTCGAC GATGCTTGGC CTACCGAAAG GAGGACCGCA TCGACGTCCA GCAGCTGGCC
    TGTGACCCCT ACTTGCTTCC TCACATCCGA AAGTCGGTCT CCACGAGTAG CCCTGCTGGA
    GCTGCTATTG CGTCAACCTC GGGGGCGTCC AATAACAGTT CTTCGAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836050.1
    CDS179..2149
    Translation

    Target ORF information:

    RefSeq Version XM_024980282.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 2 (TLK2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980282.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GCGGCGAGTA GAACAGCCTC TCTATGGTTT AGATGGCAGC
    GCTACAAAGG AGGTGTCAGA GGAGCAGTCT GCCCTGCCAA CGCTTATGTC AGTGATGCTG
    GCAAAACCTC GGCTCGACAC AGAGCAGCTG GCACAGAGGG GAGCTGGCCT CTGCTTCACT
    TTTGTCTCAG CTCAGCAAAA CAGTCCCTCA TCTACGGGAT CTGGCAACAC AGAGCATTCC
    TGCAGCTCCC AAAAACAGAT CTCCATCCAG CACAGACAGA CCCAGTCTGA CCTCACAATA
    GAAAAAATAT CTGCACTAGA AAACAGTAAG AATTCTGATT TAGAGAAGAA GGAAGGAAGA
    ATAGATGATT TATTAAGAGC CAACTGTGAT TTGAGACGGC AGATTGATGA ACAGCAAAAG
    ATGTTAGAGA AATACAAAGA ACGATTAAAT CGATGTGTGA CGATGAGCAA GAAACTGCTT
    ATAGAAAAGT CAAAACAAGA GAAGATGGCA TGTAGAGATA AAAGCATGCA AGATCGCTTG
    AGACTGGGCC ACTTTACCAC AGTCCGACAT GGGGCTTCGT TTACTGAACA GTGGACAGAT
    GGTTATGCTT TCCAGAACCT TATTAAGCAA CAGGAAAGGA TAAATTCACA GAGGGAAGAG
    ATAGAAAGAC AACGAAAAAT GTTAGCGAAG CGGAAACCTC CTGCCATGGG ACAGGCCCCT
    CCAGCAACCA ATGAGCAGAA ACAGCGGAAA AGCAAGACCA ACGGAGCTGA GAATGAAACG
    TTAACATTAG CAGAATACCA TGAACAAGAA GAAATCTTCA AACTCAGATT AGGTCATCTT
    AAAAAGGAGG AAGCAGAGAT CCAGGCAGAG CTGGAAAGGC TAGAAAGGGT TAGAAATCTA
    CATATCAGGG AACTAAAAAG GATACATAAT GAAGATAATT CACAATTTAA AGATCATCCA
    ACGCTAAATG ACAGATATTT GTTGTTACAT CTTTTGGGTA GAGGAGGTTT CAGTGAAGTT
    TACAAGGCAT TTGATCTAAC AGAACAAAGA TACGTAGCTG TGAAAATTCA CCAGTTAAAT
    AAAAACTGGA GAGATGAGAA AAAGGAGAAT TACCACAAGC ATGCATGTAG GGAATACCGG
    ATTCACAAAG AACTGGATCA TCCCAGAATA GTTAAGCTGT ATGATTACTT TTCACTGGAC
    ACTGACTCGT TTTGTACAGT ACTAGAATAC TGTGAGGGAA ATGATCTGGA CTTCTACCTG
    AAACAGCACA AATTAATGTC GGAGAAAGAA GCCCGATCCA TTATCATGCA GATTGTGAAT
    GCTTTAAAGT ACTTAAATGA AATAAAACCT CCTATCATAC ACTATGACCT CAAACCAGGT
    AATATTCTTT TAGTAAATGG TACAGCGTGT GGAGAGATAA AAATCACAGA TTTTGGTCTT
    TCTAAGATAA TGGATGATGA TAGCTACAAT TCAGTGGATG GCATGGAGCT AACATCACAA
    GGTGCTGGTA CTTACTGGTA TTTACCGCCA GAGTGTTTTG TGGTTGGGAA AGAACCACCA
    AAGATCTCAA ATAAAGTTGA CGTCTGGTCA GTGGGTGTGA TCTTCTACCA GTGTCTTTAC
    GGAAGGAAGC CTTTTGGCCA TAATCAGTCC CAGCAAGACA TTCTACAAGA AAATACTATC
    CTCAAAGCTA CTGAAGTGCA GTTCCCGCCA AAGCCAGTAG TAACACCTGA AGCAAAGGCG
    TTTATTCGAC GATGCTTGGC CTACCGAAAG GAGGACCGCA TCGACGTCCA GCAGCTGGCC
    TGTGACCCCT ACTTGCTTCC TCACATCCGA AAGTCGGTCT CCACGAGTAG CCCTGCTGGA
    GCTGCTATTG CGTCAACCTC GGGGGCGTCC AATAACAGTT CTTCGAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52918
    Clone ID Related Accession (Same CDS sequence) XM_024980279.1
    Accession Version XM_024980279.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT
    GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA
    CCGCGGCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GCGCTACAAA GGAGGTGTCA
    GAGGAGCAGT CTGCCCTGCC AACGCTTATG TCAGTGATGC TGGCAAAACC TCGGCTCGAC
    ACAGAGCAGC TGGCACAGAG GGGAGCTGGC CTCTGCTTCA CTTTTGTCTC ATCTGACCTC
    ACAATAGAAA AAATATCTGC ACTAGAAAAC AGTAAGAATT CTGATTTAGA GAAGAAGGAA
    GGAAGAATAG ATGATTTATT AAGAGCCAAC TGTGATTTGA GACGGCAGAT TGATGAACAG
    CAAAAGATGT TAGAGAAATA CAAAGAACGA TTAAATCGAT GTGTGACGAT GAGCAAGAAA
    CTGCTTATAG AAAAGTCAAA ACAAGAGAAG ATGGCATGTA GAGATAAAAG CATGCAAGAT
    CGCTTGAGAC TGGGCCACTT TACCACAGTC CGACATGGGG CTTCGTTTAC TGAACAGTGG
    ACAGATGGTT ATGCTTTCCA GAACCTTATT AAGCAACAGG AAAGGATAAA TTCACAGAGG
    GAAGAGATAG AAAGACAACG AAAAATGTTA GCGAAGCGGA AACCTCCTGC CATGGGACAG
    GCCCCTCCAG CAACCAATGA GCAGAAACAG CGGAAAAGCA AGACCAACGG AGCTGAGAAT
    GAAACGTTAA CATTAGCAGA ATACCATGAA CAAGAAGAAA TCTTCAAACT CAGATTAGGT
    CATCTTAAAA AGGAGGAAGC AGAGATCCAG GCAGAGCTGG AAAGGCTAGA AAGGGTTAGA
    AATCTACATA TCAGGGAACT AAAAAGGATA CATAATGAAG ATAATTCACA ATTTAAAGAT
    CATCCAACGC TAAATGACAG ATATTTGTTG TTACATCTTT TGGGTAGAGG AGGTTTCAGT
    GAAGTTTACA AGGCATTTGA TCTAACAGAA CAAAGATACG TAGCTGTGAA AATTCACCAG
    TTAAATAAAA ACTGGAGAGA TGAGAAAAAG GAGAATTACC ACAAGCATGC ATGTAGGGAA
    TACCGGATTC ACAAAGAACT GGATCATCCC AGAATAGTTA AGCTGTATGA TTACTTTTCA
    CTGGACACTG ACTCGTTTTG TACAGTACTA GAATACTGTG AGGGAAATGA TCTGGACTTC
    TACCTGAAAC AGCACAAATT AATGTCGGAG AAAGAAGCCC GATCCATTAT CATGCAGATT
    GTGAATGCTT TAAAGTACTT AAATGAAATA AAACCTCCTA TCATACACTA TGACCTCAAA
    CCAGGTAATA TTCTTTTAGT AAATGGTACA GCGTGTGGAG AGATAAAAAT CACAGATTTT
    GGTCTTTCTA AGATAATGGA TGATGATAGC TACAATTCAG TGGATGGCAT GGAGCTAACA
    TCACAAGGTG CTGGTACTTA CTGGTATTTA CCGCCAGAGT GTTTTGTGGT TGGGAAAGAA
    CCACCAAAGA TCTCAAATAA AGTTGACGTC TGGTCAGTGG GTGTGATCTT CTACCAGTGT
    CTTTACGGAA GGAAGCCTTT TGGCCATAAT CAGTCCCAGC AAGACATTCT ACAAGAAAAT
    ACTATCCTCA AAGCTACTGA AGTGCAGTTC CCGCCAAAGC CAGTAGTAAC ACCTGAAGCA
    AAGGCGTTTA TTCGACGATG CTTGGCCTAC CGAAAGGAGG ACCGCATCGA CGTCCAGCAG
    CTGGCCTGTG ACCCCTACTT GCTTCCTCAC ATCCGAAAGT CGGTCTCCAC GAGTAGCCCT
    GCTGGAGCTG CTATTGCGTC AACCTCGGGG GCGTCCAATA ACAGTTCTTC GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836047.1
    CDS520..2676
    Translation

    Target ORF information:

    RefSeq Version XM_024980279.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 2 (TLK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980279.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT
    GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA
    CCGCGGCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GCGCTACAAA GGAGGTGTCA
    GAGGAGCAGT CTGCCCTGCC AACGCTTATG TCAGTGATGC TGGCAAAACC TCGGCTCGAC
    ACAGAGCAGC TGGCACAGAG GGGAGCTGGC CTCTGCTTCA CTTTTGTCTC ATCTGACCTC
    ACAATAGAAA AAATATCTGC ACTAGAAAAC AGTAAGAATT CTGATTTAGA GAAGAAGGAA
    GGAAGAATAG ATGATTTATT AAGAGCCAAC TGTGATTTGA GACGGCAGAT TGATGAACAG
    CAAAAGATGT TAGAGAAATA CAAAGAACGA TTAAATCGAT GTGTGACGAT GAGCAAGAAA
    CTGCTTATAG AAAAGTCAAA ACAAGAGAAG ATGGCATGTA GAGATAAAAG CATGCAAGAT
    CGCTTGAGAC TGGGCCACTT TACCACAGTC CGACATGGGG CTTCGTTTAC TGAACAGTGG
    ACAGATGGTT ATGCTTTCCA GAACCTTATT AAGCAACAGG AAAGGATAAA TTCACAGAGG
    GAAGAGATAG AAAGACAACG AAAAATGTTA GCGAAGCGGA AACCTCCTGC CATGGGACAG
    GCCCCTCCAG CAACCAATGA GCAGAAACAG CGGAAAAGCA AGACCAACGG AGCTGAGAAT
    GAAACGTTAA CATTAGCAGA ATACCATGAA CAAGAAGAAA TCTTCAAACT CAGATTAGGT
    CATCTTAAAA AGGAGGAAGC AGAGATCCAG GCAGAGCTGG AAAGGCTAGA AAGGGTTAGA
    AATCTACATA TCAGGGAACT AAAAAGGATA CATAATGAAG ATAATTCACA ATTTAAAGAT
    CATCCAACGC TAAATGACAG ATATTTGTTG TTACATCTTT TGGGTAGAGG AGGTTTCAGT
    GAAGTTTACA AGGCATTTGA TCTAACAGAA CAAAGATACG TAGCTGTGAA AATTCACCAG
    TTAAATAAAA ACTGGAGAGA TGAGAAAAAG GAGAATTACC ACAAGCATGC ATGTAGGGAA
    TACCGGATTC ACAAAGAACT GGATCATCCC AGAATAGTTA AGCTGTATGA TTACTTTTCA
    CTGGACACTG ACTCGTTTTG TACAGTACTA GAATACTGTG AGGGAAATGA TCTGGACTTC
    TACCTGAAAC AGCACAAATT AATGTCGGAG AAAGAAGCCC GATCCATTAT CATGCAGATT
    GTGAATGCTT TAAAGTACTT AAATGAAATA AAACCTCCTA TCATACACTA TGACCTCAAA
    CCAGGTAATA TTCTTTTAGT AAATGGTACA GCGTGTGGAG AGATAAAAAT CACAGATTTT
    GGTCTTTCTA AGATAATGGA TGATGATAGC TACAATTCAG TGGATGGCAT GGAGCTAACA
    TCACAAGGTG CTGGTACTTA CTGGTATTTA CCGCCAGAGT GTTTTGTGGT TGGGAAAGAA
    CCACCAAAGA TCTCAAATAA AGTTGACGTC TGGTCAGTGG GTGTGATCTT CTACCAGTGT
    CTTTACGGAA GGAAGCCTTT TGGCCATAAT CAGTCCCAGC AAGACATTCT ACAAGAAAAT
    ACTATCCTCA AAGCTACTGA AGTGCAGTTC CCGCCAAAGC CAGTAGTAAC ACCTGAAGCA
    AAGGCGTTTA TTCGACGATG CTTGGCCTAC CGAAAGGAGG ACCGCATCGA CGTCCAGCAG
    CTGGCCTGTG ACCCCTACTT GCTTCCTCAC ATCCGAAAGT CGGTCTCCAC GAGTAGCCCT
    GCTGGAGCTG CTATTGCGTC AACCTCGGGG GCGTCCAATA ACAGTTCTTC GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52919
    Clone ID Related Accession (Same CDS sequence) XM_024980281.1
    Accession Version XM_024980281.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase tousled-like 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT
    GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA
    CCGCGGCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GCGCTACAAA GGAGGTGTCA
    GAGGAGCAGT CTGCCCTGCC AACGCTTATG TCAGTGATGC TGGCAAAACC TCGGCTCGAC
    ACAGAGCAGC TGGCACAGAG GGGAGCTGGC CTCTGCTTCA CTTTTGTCTC AGCCAACTGT
    GATTTGAGAC GGCAGATTGA TGAACAGCAA AAGATGTTAG AGAAATACAA AGAACGATTA
    AATCGATGTG TGACGATGAG CAAGAAACTG CTTATAGAAA AGTCAAAACA AGAGAAGATG
    GCATGTAGAG ATAAAAGCAT GCAAGATCGC TTGAGACTGG GCCACTTTAC CACAGTCCGA
    CATGGGGCTT CGTTTACTGA ACAGTGGACA GATGGTTATG CTTTCCAGAA CCTTATTAAG
    CAACAGGAAA GGATAAATTC ACAGAGGGAA GAGATAGAAA GACAACGAAA AATGTTAGCG
    AAGCGGAAAC CTCCTGCCAT GGGACAGGCC CCTCCAGCAA CCAATGAGCA GAAACAGCGG
    AAAAGCAAGA CCAACGGAGC TGAGAATGAA ACGTTAACAT TAGCAGAATA CCATGAACAA
    GAAGAAATCT TCAAACTCAG ATTAGGTCAT CTTAAAAAGG AGGAAGCAGA GATCCAGGCA
    GAGCTGGAAA GGCTAGAAAG GGTTAGAAAT CTACATATCA GGGAACTAAA AAGGATACAT
    AATGAAGATA ATTCACAATT TAAAGATCAT CCAACGCTAA ATGACAGATA TTTGTTGTTA
    CATCTTTTGG GTAGAGGAGG TTTCAGTGAA GTTTACAAGG CATTTGATCT AACAGAACAA
    AGATACGTAG CTGTGAAAAT TCACCAGTTA AATAAAAACT GGAGAGATGA GAAAAAGGAG
    AATTACCACA AGCATGCATG TAGGGAATAC CGGATTCACA AAGAACTGGA TCATCCCAGA
    ATAGTTAAGC TGTATGATTA CTTTTCACTG GACACTGACT CGTTTTGTAC AGTACTAGAA
    TACTGTGAGG GAAATGATCT GGACTTCTAC CTGAAACAGC ACAAATTAAT GTCGGAGAAA
    GAAGCCCGAT CCATTATCAT GCAGATTGTG AATGCTTTAA AGTACTTAAA TGAAATAAAA
    CCTCCTATCA TACACTATGA CCTCAAACCA GGTAATATTC TTTTAGTAAA TGGTACAGCG
    TGTGGAGAGA TAAAAATCAC AGATTTTGGT CTTTCTAAGA TAATGGATGA TGATAGCTAC
    AATTCAGTGG ATGGCATGGA GCTAACATCA CAAGGTGCTG GTACTTACTG GTATTTACCG
    CCAGAGTGTT TTGTGGTTGG GAAAGAACCA CCAAAGATCT CAAATAAAGT TGACGTCTGG
    TCAGTGGGTG TGATCTTCTA CCAGTGTCTT TACGGAAGGA AGCCTTTTGG CCATAATCAG
    TCCCAGCAAG ACATTCTACA AGAAAATACT ATCCTCAAAG CTACTGAAGT GCAGTTCCCG
    CCAAAGCCAG TAGTAACACC TGAAGCAAAG GCGTTTATTC GACGATGCTT GGCCTACCGA
    AAGGAGGACC GCATCGACGT CCAGCAGCTG GCCTGTGACC CCTACTTGCT TCCTCACATC
    CGAAAGTCGG TCTCCACGAG TAGCCCTGCT GGAGCTGCTA TTGCGTCAAC CTCGGGGGCG
    TCCAATAACA GTTCTTCGAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836049.1
    CDS520..2583
    Translation

    Target ORF information:

    RefSeq Version XM_024980281.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tousled like kinase 2 (TLK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980281.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGATGGAAG AATTGCATAG CCTGGACCCC CGACGGCAGG AATTATTAGA GGCCAGGTTT 
    ACTGGAGTTG GTGTTAGTAA GGGGCCACTC AATAGTGAGT CTTCCAACCA GAGCTTGTGC
    AGCGTGGGGT CCTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAACA GAATGACCAG
    CGAAACCGGA AAAGAAAAGC TGAACCATAT GAAACTAGCC AAGGGAAAGG CACTCCTAGG
    GGACATAAAA TTAGTGATTA CTTTGAGTTT GCTGGGGGAA GCGGGCCAGG AACCAGCCCT
    GGCAGAAGTG TTCCACCAGT TGCACGATCC TCACCGCAAC ATTCCTTATC CAATCCCTTA
    CCGCGGCGAG TAGAACAGCC TCTCTATGGT TTAGATGGCA GCGCTACAAA GGAGGTGTCA
    GAGGAGCAGT CTGCCCTGCC AACGCTTATG TCAGTGATGC TGGCAAAACC TCGGCTCGAC
    ACAGAGCAGC TGGCACAGAG GGGAGCTGGC CTCTGCTTCA CTTTTGTCTC AGCCAACTGT
    GATTTGAGAC GGCAGATTGA TGAACAGCAA AAGATGTTAG AGAAATACAA AGAACGATTA
    AATCGATGTG TGACGATGAG CAAGAAACTG CTTATAGAAA AGTCAAAACA AGAGAAGATG
    GCATGTAGAG ATAAAAGCAT GCAAGATCGC TTGAGACTGG GCCACTTTAC CACAGTCCGA
    CATGGGGCTT CGTTTACTGA ACAGTGGACA GATGGTTATG CTTTCCAGAA CCTTATTAAG
    CAACAGGAAA GGATAAATTC ACAGAGGGAA GAGATAGAAA GACAACGAAA AATGTTAGCG
    AAGCGGAAAC CTCCTGCCAT GGGACAGGCC CCTCCAGCAA CCAATGAGCA GAAACAGCGG
    AAAAGCAAGA CCAACGGAGC TGAGAATGAA ACGTTAACAT TAGCAGAATA CCATGAACAA
    GAAGAAATCT TCAAACTCAG ATTAGGTCAT CTTAAAAAGG AGGAAGCAGA GATCCAGGCA
    GAGCTGGAAA GGCTAGAAAG GGTTAGAAAT CTACATATCA GGGAACTAAA AAGGATACAT
    AATGAAGATA ATTCACAATT TAAAGATCAT CCAACGCTAA ATGACAGATA TTTGTTGTTA
    CATCTTTTGG GTAGAGGAGG TTTCAGTGAA GTTTACAAGG CATTTGATCT AACAGAACAA
    AGATACGTAG CTGTGAAAAT TCACCAGTTA AATAAAAACT GGAGAGATGA GAAAAAGGAG
    AATTACCACA AGCATGCATG TAGGGAATAC CGGATTCACA AAGAACTGGA TCATCCCAGA
    ATAGTTAAGC TGTATGATTA CTTTTCACTG GACACTGACT CGTTTTGTAC AGTACTAGAA
    TACTGTGAGG GAAATGATCT GGACTTCTAC CTGAAACAGC ACAAATTAAT GTCGGAGAAA
    GAAGCCCGAT CCATTATCAT GCAGATTGTG AATGCTTTAA AGTACTTAAA TGAAATAAAA
    CCTCCTATCA TACACTATGA CCTCAAACCA GGTAATATTC TTTTAGTAAA TGGTACAGCG
    TGTGGAGAGA TAAAAATCAC AGATTTTGGT CTTTCTAAGA TAATGGATGA TGATAGCTAC
    AATTCAGTGG ATGGCATGGA GCTAACATCA CAAGGTGCTG GTACTTACTG GTATTTACCG
    CCAGAGTGTT TTGTGGTTGG GAAAGAACCA CCAAAGATCT CAAATAAAGT TGACGTCTGG
    TCAGTGGGTG TGATCTTCTA CCAGTGTCTT TACGGAAGGA AGCCTTTTGG CCATAATCAG
    TCCCAGCAAG ACATTCTACA AGAAAATACT ATCCTCAAAG CTACTGAAGT GCAGTTCCCG
    CCAAAGCCAG TAGTAACACC TGAAGCAAAG GCGTTTATTC GACGATGCTT GGCCTACCGA
    AAGGAGGACC GCATCGACGT CCAGCAGCTG GCCTGTGACC CCTACTTGCT TCCTCACATC
    CGAAAGTCGG TCTCCACGAG TAGCCCTGCT GGAGCTGCTA TTGCGTCAAC CTCGGGGGCG
    TCCAATAACA GTTCTTCGAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.