Tlk cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Tlk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tlk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa02497 NM_001272292.1
Latest version!
Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OFa02498 NM_001144680.3
Latest version!
Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa02498 NM_001169184.2
Latest version!
Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa02498 NM_001144682.2
Latest version!
Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa02499 NM_001272291.1
Latest version!
Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa02500 NM_001272293.1
Latest version!
Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa02500 NM_001144683.1
Latest version!
Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa02500 NM_001297914.1
Latest version!
Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant N, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa02927 NM_206620.6
Latest version!
Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa02928 NM_001103403.1
Latest version!
Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant B, partial mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OFa02929 NM_001144681.3
Latest version!
Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OFa02930 NM_130717.5
Latest version!
Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa02497
    Clone ID Related Accession (Same CDS sequence) NM_001272292.1
    Accession Version NM_001272292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Tousled-like kinase, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGATCAAT TTCAAACTTC ACTAAATCCG CGGAAACTCG AGCTTCTGGA GTCTCGAATT 
    ACTGGAGTGC GGATGTCCCC CGGCGCCCAT TTGCAGATGT CCCCGCAGAA CACTTCGTCC
    CTAAGTCAAC ACCATCCACA TCAACAGCAA CAGTTACAAC CCCCACAGCA GCAACAACAG
    CATTTCCCTA ACCATCACAG CGCCCAGCAA CAGTCGCAGC AGCAGCAGCA ACAGGAGCAA
    CAGAATCCCC AGCAGCAGGC GCAACAGCAG CAGCAGATAC TCCCACATCA ACATTTGCAG
    CACCTGCACA AGCATCCGCA TCAGCTGCAA CTGCATCAGC AGCAGCAACA ACAACTCCAC
    CAGCAACAGC AGCAACACTT CCACCAGCAG TCGCTGCAAG GGCTGCATCA GGGTAGCAGC
    AATCCGGATT CGAATATGAG CACTGGCTCC TCGCATAGCG AGAAGGATGT CAATGATATG
    CTGAGTGGCG GTGCAGCAAC GCCAGGAGCT GCAGCAGCAG CGATTCAACA GCAACATCCC
    GCCTTTGCGC CCACACTGGG AATGCAGCAA CCACCGCCGC CCCCACCTCA ACACTCCAAT
    AATGGAGGCG AGATGGGCTA CTTGTCGGCA GGCACGACCA CGACGACGTC GGTGTTAACG
    GTAGGCAAGC CTCGGACGCC AGCGGAGCGG AAACGGAAGC GAAAAATGCC TCCATGTGCC
    ACTAGTGCGG ATGAGGCGGG GAGTGGCGGT GGCTCTGGCG GAGCAGGAGC AACCGTTGTT
    AACAACAGCA GCCTGAAGGG CAAATCATTG GCCTTTCGTG ATATGCCCAA GGTAAACATG
    AGCCTGAATC TGGGCGATCG TCTGGGAGGA TCTGCAGGAA GCGGAGTAGG AGCCGGTGGC
    GCCGGAAGCG GGGGAGGTGG CGCTGGTTCC GGTTCTGGAA GCGGTGGCGG CAAAAGCGCC
    CGCCTGATGC TGCCAGTCAG CGACAACAAG AAGATCAACG ACTATTTCAA TAAGCAGCAA
    ACGGGCGTGG GCGTCGGTGT GCCAGGTGGT GCGGGAGGCA ATACCGCTGG CCTTCGAGGA
    TCACATACGG GAGGTGGCAG CAAGTCACCC TCATCCGCCC AGCAGCAGCA AACGGCGGCA
    CAGCAGCAGG GAAGCGGTGT TGCGACGGGA GGCAGTGCAG GCGGTTCCGC TGGCAACCAG
    GTGCAAGTGC AAACGAGCAG CGCTTACGCC CTTTACCCAC CAGCTAGTCC CCAAACCCAG
    ACGTCACAGC AACAGCAGCA GCAGCAACCG GGATCAGACT TTCACTATGT CAACTCCAGC
    AAGGCGCAGC AACAACAGCA GCGTCAACAG CAACAGACTT CCAATCAAAT GGTTCCTCCA
    CACGTGGTCG TTGGCCTTGG TGGTCATCCA CTGAGCCTCG CGTCCATTCA GCAGCAGACG
    CCCTTATCCC AGCAGCAACA GCAGCAACAA CAGCAGCAGC AACAGCAGCA ACTGGGACCA
    CCGACCACAT CGACGGCCTC CGTCGTGCCA ACGCATCCGC ATCAACTCGG ATCCCTGGGA
    GTTGTTGGGA TGGTCGGTGT GGGTGTTGGC GTGGGCGTTG GAGTAAATGT GGGTGTGGGA
    CCACCACTGC CACCACCACC GCCGATGGCC ATGCCAGCGG CCATTATCAC TTATAGTAAG
    GCCACTCAAA CGGAGGTGTC GCTGCATGAA TTGCAGGAGC GCGAAGCGGA GCACGAATCG
    GGCAAGGTGA AGCTAGACGA GATGACACGG CTGTCCGATG AACAAAAGTC CCAAATTGTT
    GGCAACCAGA AGACGATTGA CCAGCACAAG TGCCACATAG CCAAGTGTAT TGATGTGGTC
    AAGAAGCTGT TGAAGGAGAA GAGCAGCATC GAGAAGAAGG AGGCGCGACA GAAGTGCATG
    CAGAATCGCC TCAGGCTCGG ACAGTTTGTT ACCCAACGAG TGGGCGCCAC ATTCCAGTCC
    CGCTTTAACA ATCATCCCGT GCTGAATGAT CGCTATCTTC TGTTGATGCT CCTGGGCAAG
    GGCGGCTTCT CAGAGGTCCA CAAGGCCTTC GACCTGAAGG AGCAACGCTA TGTCGCATGT
    AAGGTGCACC AATTAAACAA GGATTGGAAG GAGGATAAGA AAGCTAATTA TATCAAACAC
    GCTTTGCGGG AATACAACAT TCACAAGGCA CTGGATCATC CGCGGGTCGT CAAGCTATAC
    GATGTCTTCG AGATCGATGC GAATTCCTTT TGCACAGTGC TCGAATACTG TGATGGCCAC
    GATCTGGACT TCTATTTGAA GCAACATAAG ACTATACCCG AGCGTGAAGC GCGCTCGATA
    ATAATGCAGG TTGTATCTGC ACTCAAGTAT CTAAATGAGA TTAAGCCTCC AGTTATCCAC
    TACGATCTGA AGCCCGGCAA CATTCTGCTT ACCGAGGGCA ACGTCTGCGG CGAGATTAAG
    ATCACCGACT TCGGTCTGTC AAAGGTGATG GACGACGAGA ATTACAATCC CGATCACGGC
    ATGGATCTGA CCTCTCAGGG GGCGGGAACC TACTGGTATC TGCCACCCGA GTGCTTTGTC
    GTGGGCAAAA ATCCGCCGAA AATCTCCTCC AAAGTGGACG TATGGAGTGT GGGTGTTATC
    TTCTACCAGT GTCTGTACGG CAAAAAGCCC TTCGGTCACA ATCAGTCGCA GGCCACGATT
    CTCGAGGAGA ATACGATCCT GAAGGCCACC GAAGTGCAGT TCTCCAACAA GCCAACCGTT
    TCTAACGAGG CCAAGAGTTT CATTCGGGGA TGCTTGGCCT ATCGCAAGGA GGATCGCATG
    GATGTGTTCG CACTGGCCAG GCACGAGTAC ATTCAGCCAC CGATACCGAA ACATGGGCGC
    GGTTCGCTCA ATCAGCAACA GCAGGCGCAA CAACAGCAGC AGCAACAACA GCAACAGCAG
    CAGCAACAGT CGTCGACGTC ACAGGCCAAT TCTACAGGCC AGACATCTTT CTCTGCCCAC
    ATGTTTGGCA ATATGAATCA GTCGAGTTCG TCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259221.1
    CDS708..3743
    Translation

    Target ORF information:

    RefSeq Version NM_001272292.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGATCAAT TTCAAACTTC ACTAAATCCG CGGAAACTCG AGCTTCTGGA GTCTCGAATT 
    ACTGGAGTGC GGATGTCCCC CGGCGCCCAT TTGCAGATGT CCCCGCAGAA CACTTCGTCC
    CTAAGTCAAC ACCATCCACA TCAACAGCAA CAGTTACAAC CCCCACAGCA GCAACAACAG
    CATTTCCCTA ACCATCACAG CGCCCAGCAA CAGTCGCAGC AGCAGCAGCA ACAGGAGCAA
    CAGAATCCCC AGCAGCAGGC GCAACAGCAG CAGCAGATAC TCCCACATCA ACATTTGCAG
    CACCTGCACA AGCATCCGCA TCAGCTGCAA CTGCATCAGC AGCAGCAACA ACAACTCCAC
    CAGCAACAGC AGCAACACTT CCACCAGCAG TCGCTGCAAG GGCTGCATCA GGGTAGCAGC
    AATCCGGATT CGAATATGAG CACTGGCTCC TCGCATAGCG AGAAGGATGT CAATGATATG
    CTGAGTGGCG GTGCAGCAAC GCCAGGAGCT GCAGCAGCAG CGATTCAACA GCAACATCCC
    GCCTTTGCGC CCACACTGGG AATGCAGCAA CCACCGCCGC CCCCACCTCA ACACTCCAAT
    AATGGAGGCG AGATGGGCTA CTTGTCGGCA GGCACGACCA CGACGACGTC GGTGTTAACG
    GTAGGCAAGC CTCGGACGCC AGCGGAGCGG AAACGGAAGC GAAAAATGCC TCCATGTGCC
    ACTAGTGCGG ATGAGGCGGG GAGTGGCGGT GGCTCTGGCG GAGCAGGAGC AACCGTTGTT
    AACAACAGCA GCCTGAAGGG CAAATCATTG GCCTTTCGTG ATATGCCCAA GGTAAACATG
    AGCCTGAATC TGGGCGATCG TCTGGGAGGA TCTGCAGGAA GCGGAGTAGG AGCCGGTGGC
    GCCGGAAGCG GGGGAGGTGG CGCTGGTTCC GGTTCTGGAA GCGGTGGCGG CAAAAGCGCC
    CGCCTGATGC TGCCAGTCAG CGACAACAAG AAGATCAACG ACTATTTCAA TAAGCAGCAA
    ACGGGCGTGG GCGTCGGTGT GCCAGGTGGT GCGGGAGGCA ATACCGCTGG CCTTCGAGGA
    TCACATACGG GAGGTGGCAG CAAGTCACCC TCATCCGCCC AGCAGCAGCA AACGGCGGCA
    CAGCAGCAGG GAAGCGGTGT TGCGACGGGA GGCAGTGCAG GCGGTTCCGC TGGCAACCAG
    GTGCAAGTGC AAACGAGCAG CGCTTACGCC CTTTACCCAC CAGCTAGTCC CCAAACCCAG
    ACGTCACAGC AACAGCAGCA GCAGCAACCG GGATCAGACT TTCACTATGT CAACTCCAGC
    AAGGCGCAGC AACAACAGCA GCGTCAACAG CAACAGACTT CCAATCAAAT GGTTCCTCCA
    CACGTGGTCG TTGGCCTTGG TGGTCATCCA CTGAGCCTCG CGTCCATTCA GCAGCAGACG
    CCCTTATCCC AGCAGCAACA GCAGCAACAA CAGCAGCAGC AACAGCAGCA ACTGGGACCA
    CCGACCACAT CGACGGCCTC CGTCGTGCCA ACGCATCCGC ATCAACTCGG ATCCCTGGGA
    GTTGTTGGGA TGGTCGGTGT GGGTGTTGGC GTGGGCGTTG GAGTAAATGT GGGTGTGGGA
    CCACCACTGC CACCACCACC GCCGATGGCC ATGCCAGCGG CCATTATCAC TTATAGTAAG
    GCCACTCAAA CGGAGGTGTC GCTGCATGAA TTGCAGGAGC GCGAAGCGGA GCACGAATCG
    GGCAAGGTGA AGCTAGACGA GATGACACGG CTGTCCGATG AACAAAAGTC CCAAATTGTT
    GGCAACCAGA AGACGATTGA CCAGCACAAG TGCCACATAG CCAAGTGTAT TGATGTGGTC
    AAGAAGCTGT TGAAGGAGAA GAGCAGCATC GAGAAGAAGG AGGCGCGACA GAAGTGCATG
    CAGAATCGCC TCAGGCTCGG ACAGTTTGTT ACCCAACGAG TGGGCGCCAC ATTCCAGTCC
    CGCTTTAACA ATCATCCCGT GCTGAATGAT CGCTATCTTC TGTTGATGCT CCTGGGCAAG
    GGCGGCTTCT CAGAGGTCCA CAAGGCCTTC GACCTGAAGG AGCAACGCTA TGTCGCATGT
    AAGGTGCACC AATTAAACAA GGATTGGAAG GAGGATAAGA AAGCTAATTA TATCAAACAC
    GCTTTGCGGG AATACAACAT TCACAAGGCA CTGGATCATC CGCGGGTCGT CAAGCTATAC
    GATGTCTTCG AGATCGATGC GAATTCCTTT TGCACAGTGC TCGAATACTG TGATGGCCAC
    GATCTGGACT TCTATTTGAA GCAACATAAG ACTATACCCG AGCGTGAAGC GCGCTCGATA
    ATAATGCAGG TTGTATCTGC ACTCAAGTAT CTAAATGAGA TTAAGCCTCC AGTTATCCAC
    TACGATCTGA AGCCCGGCAA CATTCTGCTT ACCGAGGGCA ACGTCTGCGG CGAGATTAAG
    ATCACCGACT TCGGTCTGTC AAAGGTGATG GACGACGAGA ATTACAATCC CGATCACGGC
    ATGGATCTGA CCTCTCAGGG GGCGGGAACC TACTGGTATC TGCCACCCGA GTGCTTTGTC
    GTGGGCAAAA ATCCGCCGAA AATCTCCTCC AAAGTGGACG TATGGAGTGT GGGTGTTATC
    TTCTACCAGT GTCTGTACGG CAAAAAGCCC TTCGGTCACA ATCAGTCGCA GGCCACGATT
    CTCGAGGAGA ATACGATCCT GAAGGCCACC GAAGTGCAGT TCTCCAACAA GCCAACCGTT
    TCTAACGAGG CCAAGAGTTT CATTCGGGGA TGCTTGGCCT ATCGCAAGGA GGATCGCATG
    GATGTGTTCG CACTGGCCAG GCACGAGTAC ATTCAGCCAC CGATACCGAA ACATGGGCGC
    GGTTCGCTCA ATCAGCAACA GCAGGCGCAA CAACAGCAGC AGCAACAACA GCAACAGCAG
    CAGCAACAGT CGTCGACGTC ACAGGCCAAT TCTACAGGCC AGACATCTTT CTCTGCCCAC
    ATGTTTGGCA ATATGAATCA GTCGAGTTCG TCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02498
    Clone ID Related Accession (Same CDS sequence) NM_001144682.2 , NM_001169184.2 , NM_001144680.3
    Accession Version NM_001144682.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Tousled-like kinase, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001144682.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCAAT TTCAAACTTC ACTAAATCCG CGGAAACTCG AGCTTCTGGA GTCTCGAATT 
    ACTGGAGTGC GGATGTCCCC CGGCGCCCAT TTGCAGATGT CCCCGCAGAA CACTTCGTCC
    CTAAGTCAAC ACCATCCACA TCAACAGCAA CAGTTACAAC CCCCACAGCA GCAACAACAG
    CATTTCCCTA ACCATCACAG CGCCCAGCAA CAGTCGCAGC AGCAGCAGCA ACAGGAGCAA
    CAGAATCCCC AGCAGCAGGC GCAACAGCAG CAGCAGATAC TCCCACATCA ACATTTGCAG
    CACCTGCACA AGCATCCGCA TCAGCTGCAA CTGCATCAGC AGCAGCAACA ACAACTCCAC
    CAGCAACAGC AGCAACACTT CCACCAGCAG TCGCTGCAAG GGCTGCATCA GGGTAGCAGC
    AATCCGGATT CGAATATGAG CACTGGCTCC TCGCATAGCG AGAAGGATGT CAATGATATG
    CTGAGTGGCG GTGCAGCAAC GCCAGGAGCT GCAGCAGCAG CGATTCAACA GCAACATCCC
    GCCTTTGCGC CCACACTGGG AATGCAGCAA CCACCGCCGC CCCCACCTCA ACACTCCAAT
    AATGGAGGCG AGATGGGCTA CTTGTCGGCA GGCACGACCA CGACGACGTC GGTGTTAACG
    GTAGGCAAGC CTCGGACGCC AGCGGAGCGG AAACGGAAGC GAAAAATGCC TCCATGTGCC
    ACTAGTGCGG ATGAGGCGGG GAGTGGCGGT GGCTCTGGCG GAGCAGGAGC AACCGTTGTT
    AACAACAGCA GCCTGAAGGG CAAATCATTG GCCTTTCGTG ATATGCCCAA GGTAAACATG
    AGCCTGAATC TGGGCGATCG TCTGGGAGGA TCTGCAGGAA GCGGAGTAGG AGCCGGTGGC
    GCCGGAAGCG GGGGAGGTGG CGCTGGTTCC GGTTCTGGAA GCGGTGGCGG CAAAAGCGCC
    CGCCTGATGC TGCCAGTCAG CGACAACAAG AAGATCAACG ACTATTTCAA TAAGCAGCAA
    ACGGGCGTGG GCGTCGGTGT GCCAGGTGGT GCGGGAGGCA ATACCGCTGG CCTTCGAGGA
    TCACATACGG GAGGTGGCAG CAAGTCACCC TCATCCGCCC AGCAGCAGCA AACGGCGGCA
    CAGCAGCAGG GAAGCGGTGT TGCGACGGGA GGCAGTGCAG GCGGTTCCGC TGGCAACCAG
    GTGCAAGTGC AAACGAGCAG CGCTTACGCC CTTTACCCAC CAGCTAGTCC CCAAACCCAG
    ACGTCACAGC AACAGCAGCA GCAGCAACCG GGATCAGACT TTCACTATGT CAACTCCAGC
    AAGGCGCAGC AACAACAGCA GCGTCAACAG CAACAGACTT CCAATCAAAT GGTTCCTCCA
    CACGTGGTCG TTGGCCTTGG TGGTCATCCA CTGAGCCTCG CGTCCATTCA GCAGCAGACG
    CCCTTATCCC AGCAGCAACA GCAGCAACAA CAGCAGCAGC AACAGCAGCA ACTGGGACCA
    CCGACCACAT CGACGGCCTC CGTCGTGCCA ACGCATCCGC ATCAACTCGG ATCCCTGGGA
    GTTGTTGGGA TGGTCGGTGT GGGTGTTGGC GTGGGCGTTG GAGTAAATGT GGGTGTGGGA
    CCACCACTGC CACCACCACC GCCGATGGCC ATGCCAGCGG CCATTATCAC TTATAGTAAG
    GCCACTCAAA CGGAGGTGTC GCTGCATGAA TTGCAGGAGC GCGAAGCGGA GCACGAATCG
    GGCAAGGTGA AGCTAGACGA GATGACACGG CTGTCCGATG AACAAAAGTC CCAAATTGTT
    GGCAACCAGA AGACGATTGA CCAGCACAAG TGCCACATAG CCAAGTGTAT TGATGTGGTC
    AAGAAGCTGT TGAAGGAGAA GAGCAGCATC GAGAAGAAGG AGGCGCGACA GAAGTGCATG
    CAGAATCGCC TCAGGCTCGG ACAGTTTGTT ACCCAACGAG TGGGCGCCAC ATTCCAGGAG
    AACTGGACGG ACGGCTATGC GTTCCAGGAG CTGAGTCGGC GGCAAGAAGA AATAACCGCT
    GAGCGTGAAG AGATAGATCG GCAGAAAAAG CAGCTGATGA AAAAGCGTCC GGCGGAGTCC
    GGACGCAAGC GCAACAACAA CAGTAACCAG AACAACCAGC AGCAGCAGCA ACAGCAACAC
    CAGCAACAGC AGCAGCAACA AAATTCCAAC TCGAACGATT CCACGCAGCT GACGAGCGGA
    GTTGTTACCG GTCCAGGCAG TGATCGTGTG AGCGTAAGCG TCGACAGCGG ATTGGGTGGC
    AATAATGCGG GCGCGATCGG TGGCGGAACC GTTGGTGGTG GCGTTGGAGG TGGTGGTGTT
    GGAGGCGGTG GTGTCGGAGG CGGCGGTGGA CGTGGACTTT CTCGCAGCAA TTCGACGCAG
    GCCAATCAGG CTCAATTGCT GCACAACGGC GGTGGTGGTT CGGGCGGCAA TGTCGGCAAC
    TCGGGCGGCG TTGGCGACCG CTTGTCAGAT CGAGGAGGAG GAGGTGGCGG CATCGGCGGA
    AACGATAGCG GCAGCTGCTC GGACTCGGGC ACTTTCCTGA AGCCAGACCC CGTATCGGGT
    GCCTACACAG CGCAGGAGTA TTACGAGTAC GATGAGATCC TCAAGTTGCG ACAAAATGCC
    CTCAAAAAGG AGGACGCCGA CCTGCAGCTG GAGATGGAGA AGCTGGAGCG GGAGCGCAAT
    CTGCACATCC GAGAGCTCAA GCGGATTCTT AACGAGGATC AGTCCCGCTT TAACAATCAT
    CCCGTGCTGA ATGATCGCTA TCTTCTGTTG ATGCTCCTGG GCAAGGGCGG CTTCTCAGAG
    GTCCACAAGG CCTTCGACCT GAAGGAGCAA CGCTATGTCG CATGTAAGGT GCACCAATTA
    AACAAGGATT GGAAGGAGGA TAAGAAAGCT AATTATATCA AACACGCTTT GCGGGAATAC
    AACATTCACA AGGCACTGGA TCATCCGCGG GTCGTCAAGC TATACGATGT CTTCGAGATC
    GATGCGAATT CCTTTTGCAC AGTGCTCGAA TACTGTGATG GCCACGATCT GGACTTCTAT
    TTGAAGCAAC ATAAGACTAT ACCCGAGCGT GAAGCGCGCT CGATAATAAT GCAGGTTGTA
    TCTGCACTCA AGTATCTAAA TGAGATTAAG CCTCCAGTTA TCCACTACGA TCTGAAGCCC
    GGCAACATTC TGCTTACCGA GGGCAACGTC TGCGGCGAGA TTAAGATCAC CGACTTCGGT
    CTGTCAAAGG TGATGGACGA CGAGAATTAC AATCCCGATC ACGGCATGGA TCTGACCTCT
    CAGGGGGCGG GAACCTACTG GTATCTGCCA CCCGAGTGCT TTGTCGTGGG CAAAAATCCG
    CCGAAAATCT CCTCCAAAGT GGACGTATGG AGTGTGGGTG TTATCTTCTA CCAGTGTCTG
    TACGGCAAAA AGCCCTTCGG TCACAATCAG TCGCAGGCCA CGATTCTCGA GGAGAATACG
    ATCCTGAAGG CCACCGAAGT GCAGTTCTCC AACAAGCCAA CCGTTTCTAA CGAGGCCAAG
    AGTTTCATTC GGGGATGCTT GGCCTATCGC AAGGAGGATC GCATGGATGT GTTCGCACTG
    GCCAGGCACG AGTACATTCA GCCACCGATA CCGAAACATG GGCGCGGTTC GCTCAATCAG
    CAACAGCAGG CGCAACAACA GCAGCAGCAA CAACAGCAAC AGCAGCAGCA ACAGTCGTCG
    ACGTCACAGG CCAATTCTAC AGGCCAGACA TCTTTCTCTG CCCACATGTT TGGCAATATG
    AATCAGTCGA GTTCGTCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138154.1
    CDS517..4317
    Translation

    Target ORF information:

    RefSeq Version NM_001144682.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144682.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCAAT TTCAAACTTC ACTAAATCCG CGGAAACTCG AGCTTCTGGA GTCTCGAATT 
    ACTGGAGTGC GGATGTCCCC CGGCGCCCAT TTGCAGATGT CCCCGCAGAA CACTTCGTCC
    CTAAGTCAAC ACCATCCACA TCAACAGCAA CAGTTACAAC CCCCACAGCA GCAACAACAG
    CATTTCCCTA ACCATCACAG CGCCCAGCAA CAGTCGCAGC AGCAGCAGCA ACAGGAGCAA
    CAGAATCCCC AGCAGCAGGC GCAACAGCAG CAGCAGATAC TCCCACATCA ACATTTGCAG
    CACCTGCACA AGCATCCGCA TCAGCTGCAA CTGCATCAGC AGCAGCAACA ACAACTCCAC
    CAGCAACAGC AGCAACACTT CCACCAGCAG TCGCTGCAAG GGCTGCATCA GGGTAGCAGC
    AATCCGGATT CGAATATGAG CACTGGCTCC TCGCATAGCG AGAAGGATGT CAATGATATG
    CTGAGTGGCG GTGCAGCAAC GCCAGGAGCT GCAGCAGCAG CGATTCAACA GCAACATCCC
    GCCTTTGCGC CCACACTGGG AATGCAGCAA CCACCGCCGC CCCCACCTCA ACACTCCAAT
    AATGGAGGCG AGATGGGCTA CTTGTCGGCA GGCACGACCA CGACGACGTC GGTGTTAACG
    GTAGGCAAGC CTCGGACGCC AGCGGAGCGG AAACGGAAGC GAAAAATGCC TCCATGTGCC
    ACTAGTGCGG ATGAGGCGGG GAGTGGCGGT GGCTCTGGCG GAGCAGGAGC AACCGTTGTT
    AACAACAGCA GCCTGAAGGG CAAATCATTG GCCTTTCGTG ATATGCCCAA GGTAAACATG
    AGCCTGAATC TGGGCGATCG TCTGGGAGGA TCTGCAGGAA GCGGAGTAGG AGCCGGTGGC
    GCCGGAAGCG GGGGAGGTGG CGCTGGTTCC GGTTCTGGAA GCGGTGGCGG CAAAAGCGCC
    CGCCTGATGC TGCCAGTCAG CGACAACAAG AAGATCAACG ACTATTTCAA TAAGCAGCAA
    ACGGGCGTGG GCGTCGGTGT GCCAGGTGGT GCGGGAGGCA ATACCGCTGG CCTTCGAGGA
    TCACATACGG GAGGTGGCAG CAAGTCACCC TCATCCGCCC AGCAGCAGCA AACGGCGGCA
    CAGCAGCAGG GAAGCGGTGT TGCGACGGGA GGCAGTGCAG GCGGTTCCGC TGGCAACCAG
    GTGCAAGTGC AAACGAGCAG CGCTTACGCC CTTTACCCAC CAGCTAGTCC CCAAACCCAG
    ACGTCACAGC AACAGCAGCA GCAGCAACCG GGATCAGACT TTCACTATGT CAACTCCAGC
    AAGGCGCAGC AACAACAGCA GCGTCAACAG CAACAGACTT CCAATCAAAT GGTTCCTCCA
    CACGTGGTCG TTGGCCTTGG TGGTCATCCA CTGAGCCTCG CGTCCATTCA GCAGCAGACG
    CCCTTATCCC AGCAGCAACA GCAGCAACAA CAGCAGCAGC AACAGCAGCA ACTGGGACCA
    CCGACCACAT CGACGGCCTC CGTCGTGCCA ACGCATCCGC ATCAACTCGG ATCCCTGGGA
    GTTGTTGGGA TGGTCGGTGT GGGTGTTGGC GTGGGCGTTG GAGTAAATGT GGGTGTGGGA
    CCACCACTGC CACCACCACC GCCGATGGCC ATGCCAGCGG CCATTATCAC TTATAGTAAG
    GCCACTCAAA CGGAGGTGTC GCTGCATGAA TTGCAGGAGC GCGAAGCGGA GCACGAATCG
    GGCAAGGTGA AGCTAGACGA GATGACACGG CTGTCCGATG AACAAAAGTC CCAAATTGTT
    GGCAACCAGA AGACGATTGA CCAGCACAAG TGCCACATAG CCAAGTGTAT TGATGTGGTC
    AAGAAGCTGT TGAAGGAGAA GAGCAGCATC GAGAAGAAGG AGGCGCGACA GAAGTGCATG
    CAGAATCGCC TCAGGCTCGG ACAGTTTGTT ACCCAACGAG TGGGCGCCAC ATTCCAGGAG
    AACTGGACGG ACGGCTATGC GTTCCAGGAG CTGAGTCGGC GGCAAGAAGA AATAACCGCT
    GAGCGTGAAG AGATAGATCG GCAGAAAAAG CAGCTGATGA AAAAGCGTCC GGCGGAGTCC
    GGACGCAAGC GCAACAACAA CAGTAACCAG AACAACCAGC AGCAGCAGCA ACAGCAACAC
    CAGCAACAGC AGCAGCAACA AAATTCCAAC TCGAACGATT CCACGCAGCT GACGAGCGGA
    GTTGTTACCG GTCCAGGCAG TGATCGTGTG AGCGTAAGCG TCGACAGCGG ATTGGGTGGC
    AATAATGCGG GCGCGATCGG TGGCGGAACC GTTGGTGGTG GCGTTGGAGG TGGTGGTGTT
    GGAGGCGGTG GTGTCGGAGG CGGCGGTGGA CGTGGACTTT CTCGCAGCAA TTCGACGCAG
    GCCAATCAGG CTCAATTGCT GCACAACGGC GGTGGTGGTT CGGGCGGCAA TGTCGGCAAC
    TCGGGCGGCG TTGGCGACCG CTTGTCAGAT CGAGGAGGAG GAGGTGGCGG CATCGGCGGA
    AACGATAGCG GCAGCTGCTC GGACTCGGGC ACTTTCCTGA AGCCAGACCC CGTATCGGGT
    GCCTACACAG CGCAGGAGTA TTACGAGTAC GATGAGATCC TCAAGTTGCG ACAAAATGCC
    CTCAAAAAGG AGGACGCCGA CCTGCAGCTG GAGATGGAGA AGCTGGAGCG GGAGCGCAAT
    CTGCACATCC GAGAGCTCAA GCGGATTCTT AACGAGGATC AGTCCCGCTT TAACAATCAT
    CCCGTGCTGA ATGATCGCTA TCTTCTGTTG ATGCTCCTGG GCAAGGGCGG CTTCTCAGAG
    GTCCACAAGG CCTTCGACCT GAAGGAGCAA CGCTATGTCG CATGTAAGGT GCACCAATTA
    AACAAGGATT GGAAGGAGGA TAAGAAAGCT AATTATATCA AACACGCTTT GCGGGAATAC
    AACATTCACA AGGCACTGGA TCATCCGCGG GTCGTCAAGC TATACGATGT CTTCGAGATC
    GATGCGAATT CCTTTTGCAC AGTGCTCGAA TACTGTGATG GCCACGATCT GGACTTCTAT
    TTGAAGCAAC ATAAGACTAT ACCCGAGCGT GAAGCGCGCT CGATAATAAT GCAGGTTGTA
    TCTGCACTCA AGTATCTAAA TGAGATTAAG CCTCCAGTTA TCCACTACGA TCTGAAGCCC
    GGCAACATTC TGCTTACCGA GGGCAACGTC TGCGGCGAGA TTAAGATCAC CGACTTCGGT
    CTGTCAAAGG TGATGGACGA CGAGAATTAC AATCCCGATC ACGGCATGGA TCTGACCTCT
    CAGGGGGCGG GAACCTACTG GTATCTGCCA CCCGAGTGCT TTGTCGTGGG CAAAAATCCG
    CCGAAAATCT CCTCCAAAGT GGACGTATGG AGTGTGGGTG TTATCTTCTA CCAGTGTCTG
    TACGGCAAAA AGCCCTTCGG TCACAATCAG TCGCAGGCCA CGATTCTCGA GGAGAATACG
    ATCCTGAAGG CCACCGAAGT GCAGTTCTCC AACAAGCCAA CCGTTTCTAA CGAGGCCAAG
    AGTTTCATTC GGGGATGCTT GGCCTATCGC AAGGAGGATC GCATGGATGT GTTCGCACTG
    GCCAGGCACG AGTACATTCA GCCACCGATA CCGAAACATG GGCGCGGTTC GCTCAATCAG
    CAACAGCAGG CGCAACAACA GCAGCAGCAA CAACAGCAAC AGCAGCAGCA ACAGTCGTCG
    ACGTCACAGG CCAATTCTAC AGGCCAGACA TCTTTCTCTG CCCACATGTT TGGCAATATG
    AATCAGTCGA GTTCGTCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02498
    Clone ID Related Accession (Same CDS sequence) NM_001144682.2 , NM_001169184.2 , NM_001144680.3
    Accession Version NM_001169184.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Tousled-like kinase, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001169184.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCAAT TTCAAACTTC ACTAAATCCG CGGAAACTCG AGCTTCTGGA GTCTCGAATT 
    ACTGGAGTGC GGATGTCCCC CGGCGCCCAT TTGCAGATGT CCCCGCAGAA CACTTCGTCC
    CTAAGTCAAC ACCATCCACA TCAACAGCAA CAGTTACAAC CCCCACAGCA GCAACAACAG
    CATTTCCCTA ACCATCACAG CGCCCAGCAA CAGTCGCAGC AGCAGCAGCA ACAGGAGCAA
    CAGAATCCCC AGCAGCAGGC GCAACAGCAG CAGCAGATAC TCCCACATCA ACATTTGCAG
    CACCTGCACA AGCATCCGCA TCAGCTGCAA CTGCATCAGC AGCAGCAACA ACAACTCCAC
    CAGCAACAGC AGCAACACTT CCACCAGCAG TCGCTGCAAG GGCTGCATCA GGGTAGCAGC
    AATCCGGATT CGAATATGAG CACTGGCTCC TCGCATAGCG AGAAGGATGT CAATGATATG
    CTGAGTGGCG GTGCAGCAAC GCCAGGAGCT GCAGCAGCAG CGATTCAACA GCAACATCCC
    GCCTTTGCGC CCACACTGGG AATGCAGCAA CCACCGCCGC CCCCACCTCA ACACTCCAAT
    AATGGAGGCG AGATGGGCTA CTTGTCGGCA GGCACGACCA CGACGACGTC GGTGTTAACG
    GTAGGCAAGC CTCGGACGCC AGCGGAGCGG AAACGGAAGC GAAAAATGCC TCCATGTGCC
    ACTAGTGCGG ATGAGGCGGG GAGTGGCGGT GGCTCTGGCG GAGCAGGAGC AACCGTTGTT
    AACAACAGCA GCCTGAAGGG CAAATCATTG GCCTTTCGTG ATATGCCCAA GGTAAACATG
    AGCCTGAATC TGGGCGATCG TCTGGGAGGA TCTGCAGGAA GCGGAGTAGG AGCCGGTGGC
    GCCGGAAGCG GGGGAGGTGG CGCTGGTTCC GGTTCTGGAA GCGGTGGCGG CAAAAGCGCC
    CGCCTGATGC TGCCAGTCAG CGACAACAAG AAGATCAACG ACTATTTCAA TAAGCAGCAA
    ACGGGCGTGG GCGTCGGTGT GCCAGGTGGT GCGGGAGGCA ATACCGCTGG CCTTCGAGGA
    TCACATACGG GAGGTGGCAG CAAGTCACCC TCATCCGCCC AGCAGCAGCA AACGGCGGCA
    CAGCAGCAGG GAAGCGGTGT TGCGACGGGA GGCAGTGCAG GCGGTTCCGC TGGCAACCAG
    GTGCAAGTGC AAACGAGCAG CGCTTACGCC CTTTACCCAC CAGCTAGTCC CCAAACCCAG
    ACGTCACAGC AACAGCAGCA GCAGCAACCG GGATCAGACT TTCACTATGT CAACTCCAGC
    AAGGCGCAGC AACAACAGCA GCGTCAACAG CAACAGACTT CCAATCAAAT GGTTCCTCCA
    CACGTGGTCG TTGGCCTTGG TGGTCATCCA CTGAGCCTCG CGTCCATTCA GCAGCAGACG
    CCCTTATCCC AGCAGCAACA GCAGCAACAA CAGCAGCAGC AACAGCAGCA ACTGGGACCA
    CCGACCACAT CGACGGCCTC CGTCGTGCCA ACGCATCCGC ATCAACTCGG ATCCCTGGGA
    GTTGTTGGGA TGGTCGGTGT GGGTGTTGGC GTGGGCGTTG GAGTAAATGT GGGTGTGGGA
    CCACCACTGC CACCACCACC GCCGATGGCC ATGCCAGCGG CCATTATCAC TTATAGTAAG
    GCCACTCAAA CGGAGGTGTC GCTGCATGAA TTGCAGGAGC GCGAAGCGGA GCACGAATCG
    GGCAAGGTGA AGCTAGACGA GATGACACGG CTGTCCGATG AACAAAAGTC CCAAATTGTT
    GGCAACCAGA AGACGATTGA CCAGCACAAG TGCCACATAG CCAAGTGTAT TGATGTGGTC
    AAGAAGCTGT TGAAGGAGAA GAGCAGCATC GAGAAGAAGG AGGCGCGACA GAAGTGCATG
    CAGAATCGCC TCAGGCTCGG ACAGTTTGTT ACCCAACGAG TGGGCGCCAC ATTCCAGGAG
    AACTGGACGG ACGGCTATGC GTTCCAGGAG CTGAGTCGGC GGCAAGAAGA AATAACCGCT
    GAGCGTGAAG AGATAGATCG GCAGAAAAAG CAGCTGATGA AAAAGCGTCC GGCGGAGTCC
    GGACGCAAGC GCAACAACAA CAGTAACCAG AACAACCAGC AGCAGCAGCA ACAGCAACAC
    CAGCAACAGC AGCAGCAACA AAATTCCAAC TCGAACGATT CCACGCAGCT GACGAGCGGA
    GTTGTTACCG GTCCAGGCAG TGATCGTGTG AGCGTAAGCG TCGACAGCGG ATTGGGTGGC
    AATAATGCGG GCGCGATCGG TGGCGGAACC GTTGGTGGTG GCGTTGGAGG TGGTGGTGTT
    GGAGGCGGTG GTGTCGGAGG CGGCGGTGGA CGTGGACTTT CTCGCAGCAA TTCGACGCAG
    GCCAATCAGG CTCAATTGCT GCACAACGGC GGTGGTGGTT CGGGCGGCAA TGTCGGCAAC
    TCGGGCGGCG TTGGCGACCG CTTGTCAGAT CGAGGAGGAG GAGGTGGCGG CATCGGCGGA
    AACGATAGCG GCAGCTGCTC GGACTCGGGC ACTTTCCTGA AGCCAGACCC CGTATCGGGT
    GCCTACACAG CGCAGGAGTA TTACGAGTAC GATGAGATCC TCAAGTTGCG ACAAAATGCC
    CTCAAAAAGG AGGACGCCGA CCTGCAGCTG GAGATGGAGA AGCTGGAGCG GGAGCGCAAT
    CTGCACATCC GAGAGCTCAA GCGGATTCTT AACGAGGATC AGTCCCGCTT TAACAATCAT
    CCCGTGCTGA ATGATCGCTA TCTTCTGTTG ATGCTCCTGG GCAAGGGCGG CTTCTCAGAG
    GTCCACAAGG CCTTCGACCT GAAGGAGCAA CGCTATGTCG CATGTAAGGT GCACCAATTA
    AACAAGGATT GGAAGGAGGA TAAGAAAGCT AATTATATCA AACACGCTTT GCGGGAATAC
    AACATTCACA AGGCACTGGA TCATCCGCGG GTCGTCAAGC TATACGATGT CTTCGAGATC
    GATGCGAATT CCTTTTGCAC AGTGCTCGAA TACTGTGATG GCCACGATCT GGACTTCTAT
    TTGAAGCAAC ATAAGACTAT ACCCGAGCGT GAAGCGCGCT CGATAATAAT GCAGGTTGTA
    TCTGCACTCA AGTATCTAAA TGAGATTAAG CCTCCAGTTA TCCACTACGA TCTGAAGCCC
    GGCAACATTC TGCTTACCGA GGGCAACGTC TGCGGCGAGA TTAAGATCAC CGACTTCGGT
    CTGTCAAAGG TGATGGACGA CGAGAATTAC AATCCCGATC ACGGCATGGA TCTGACCTCT
    CAGGGGGCGG GAACCTACTG GTATCTGCCA CCCGAGTGCT TTGTCGTGGG CAAAAATCCG
    CCGAAAATCT CCTCCAAAGT GGACGTATGG AGTGTGGGTG TTATCTTCTA CCAGTGTCTG
    TACGGCAAAA AGCCCTTCGG TCACAATCAG TCGCAGGCCA CGATTCTCGA GGAGAATACG
    ATCCTGAAGG CCACCGAAGT GCAGTTCTCC AACAAGCCAA CCGTTTCTAA CGAGGCCAAG
    AGTTTCATTC GGGGATGCTT GGCCTATCGC AAGGAGGATC GCATGGATGT GTTCGCACTG
    GCCAGGCACG AGTACATTCA GCCACCGATA CCGAAACATG GGCGCGGTTC GCTCAATCAG
    CAACAGCAGG CGCAACAACA GCAGCAGCAA CAACAGCAAC AGCAGCAGCA ACAGTCGTCG
    ACGTCACAGG CCAATTCTAC AGGCCAGACA TCTTTCTCTG CCCACATGTT TGGCAATATG
    AATCAGTCGA GTTCGTCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162655.1
    CDS517..4317
    Translation

    Target ORF information:

    RefSeq Version NM_001169184.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169184.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCAAT TTCAAACTTC ACTAAATCCG CGGAAACTCG AGCTTCTGGA GTCTCGAATT 
    ACTGGAGTGC GGATGTCCCC CGGCGCCCAT TTGCAGATGT CCCCGCAGAA CACTTCGTCC
    CTAAGTCAAC ACCATCCACA TCAACAGCAA CAGTTACAAC CCCCACAGCA GCAACAACAG
    CATTTCCCTA ACCATCACAG CGCCCAGCAA CAGTCGCAGC AGCAGCAGCA ACAGGAGCAA
    CAGAATCCCC AGCAGCAGGC GCAACAGCAG CAGCAGATAC TCCCACATCA ACATTTGCAG
    CACCTGCACA AGCATCCGCA TCAGCTGCAA CTGCATCAGC AGCAGCAACA ACAACTCCAC
    CAGCAACAGC AGCAACACTT CCACCAGCAG TCGCTGCAAG GGCTGCATCA GGGTAGCAGC
    AATCCGGATT CGAATATGAG CACTGGCTCC TCGCATAGCG AGAAGGATGT CAATGATATG
    CTGAGTGGCG GTGCAGCAAC GCCAGGAGCT GCAGCAGCAG CGATTCAACA GCAACATCCC
    GCCTTTGCGC CCACACTGGG AATGCAGCAA CCACCGCCGC CCCCACCTCA ACACTCCAAT
    AATGGAGGCG AGATGGGCTA CTTGTCGGCA GGCACGACCA CGACGACGTC GGTGTTAACG
    GTAGGCAAGC CTCGGACGCC AGCGGAGCGG AAACGGAAGC GAAAAATGCC TCCATGTGCC
    ACTAGTGCGG ATGAGGCGGG GAGTGGCGGT GGCTCTGGCG GAGCAGGAGC AACCGTTGTT
    AACAACAGCA GCCTGAAGGG CAAATCATTG GCCTTTCGTG ATATGCCCAA GGTAAACATG
    AGCCTGAATC TGGGCGATCG TCTGGGAGGA TCTGCAGGAA GCGGAGTAGG AGCCGGTGGC
    GCCGGAAGCG GGGGAGGTGG CGCTGGTTCC GGTTCTGGAA GCGGTGGCGG CAAAAGCGCC
    CGCCTGATGC TGCCAGTCAG CGACAACAAG AAGATCAACG ACTATTTCAA TAAGCAGCAA
    ACGGGCGTGG GCGTCGGTGT GCCAGGTGGT GCGGGAGGCA ATACCGCTGG CCTTCGAGGA
    TCACATACGG GAGGTGGCAG CAAGTCACCC TCATCCGCCC AGCAGCAGCA AACGGCGGCA
    CAGCAGCAGG GAAGCGGTGT TGCGACGGGA GGCAGTGCAG GCGGTTCCGC TGGCAACCAG
    GTGCAAGTGC AAACGAGCAG CGCTTACGCC CTTTACCCAC CAGCTAGTCC CCAAACCCAG
    ACGTCACAGC AACAGCAGCA GCAGCAACCG GGATCAGACT TTCACTATGT CAACTCCAGC
    AAGGCGCAGC AACAACAGCA GCGTCAACAG CAACAGACTT CCAATCAAAT GGTTCCTCCA
    CACGTGGTCG TTGGCCTTGG TGGTCATCCA CTGAGCCTCG CGTCCATTCA GCAGCAGACG
    CCCTTATCCC AGCAGCAACA GCAGCAACAA CAGCAGCAGC AACAGCAGCA ACTGGGACCA
    CCGACCACAT CGACGGCCTC CGTCGTGCCA ACGCATCCGC ATCAACTCGG ATCCCTGGGA
    GTTGTTGGGA TGGTCGGTGT GGGTGTTGGC GTGGGCGTTG GAGTAAATGT GGGTGTGGGA
    CCACCACTGC CACCACCACC GCCGATGGCC ATGCCAGCGG CCATTATCAC TTATAGTAAG
    GCCACTCAAA CGGAGGTGTC GCTGCATGAA TTGCAGGAGC GCGAAGCGGA GCACGAATCG
    GGCAAGGTGA AGCTAGACGA GATGACACGG CTGTCCGATG AACAAAAGTC CCAAATTGTT
    GGCAACCAGA AGACGATTGA CCAGCACAAG TGCCACATAG CCAAGTGTAT TGATGTGGTC
    AAGAAGCTGT TGAAGGAGAA GAGCAGCATC GAGAAGAAGG AGGCGCGACA GAAGTGCATG
    CAGAATCGCC TCAGGCTCGG ACAGTTTGTT ACCCAACGAG TGGGCGCCAC ATTCCAGGAG
    AACTGGACGG ACGGCTATGC GTTCCAGGAG CTGAGTCGGC GGCAAGAAGA AATAACCGCT
    GAGCGTGAAG AGATAGATCG GCAGAAAAAG CAGCTGATGA AAAAGCGTCC GGCGGAGTCC
    GGACGCAAGC GCAACAACAA CAGTAACCAG AACAACCAGC AGCAGCAGCA ACAGCAACAC
    CAGCAACAGC AGCAGCAACA AAATTCCAAC TCGAACGATT CCACGCAGCT GACGAGCGGA
    GTTGTTACCG GTCCAGGCAG TGATCGTGTG AGCGTAAGCG TCGACAGCGG ATTGGGTGGC
    AATAATGCGG GCGCGATCGG TGGCGGAACC GTTGGTGGTG GCGTTGGAGG TGGTGGTGTT
    GGAGGCGGTG GTGTCGGAGG CGGCGGTGGA CGTGGACTTT CTCGCAGCAA TTCGACGCAG
    GCCAATCAGG CTCAATTGCT GCACAACGGC GGTGGTGGTT CGGGCGGCAA TGTCGGCAAC
    TCGGGCGGCG TTGGCGACCG CTTGTCAGAT CGAGGAGGAG GAGGTGGCGG CATCGGCGGA
    AACGATAGCG GCAGCTGCTC GGACTCGGGC ACTTTCCTGA AGCCAGACCC CGTATCGGGT
    GCCTACACAG CGCAGGAGTA TTACGAGTAC GATGAGATCC TCAAGTTGCG ACAAAATGCC
    CTCAAAAAGG AGGACGCCGA CCTGCAGCTG GAGATGGAGA AGCTGGAGCG GGAGCGCAAT
    CTGCACATCC GAGAGCTCAA GCGGATTCTT AACGAGGATC AGTCCCGCTT TAACAATCAT
    CCCGTGCTGA ATGATCGCTA TCTTCTGTTG ATGCTCCTGG GCAAGGGCGG CTTCTCAGAG
    GTCCACAAGG CCTTCGACCT GAAGGAGCAA CGCTATGTCG CATGTAAGGT GCACCAATTA
    AACAAGGATT GGAAGGAGGA TAAGAAAGCT AATTATATCA AACACGCTTT GCGGGAATAC
    AACATTCACA AGGCACTGGA TCATCCGCGG GTCGTCAAGC TATACGATGT CTTCGAGATC
    GATGCGAATT CCTTTTGCAC AGTGCTCGAA TACTGTGATG GCCACGATCT GGACTTCTAT
    TTGAAGCAAC ATAAGACTAT ACCCGAGCGT GAAGCGCGCT CGATAATAAT GCAGGTTGTA
    TCTGCACTCA AGTATCTAAA TGAGATTAAG CCTCCAGTTA TCCACTACGA TCTGAAGCCC
    GGCAACATTC TGCTTACCGA GGGCAACGTC TGCGGCGAGA TTAAGATCAC CGACTTCGGT
    CTGTCAAAGG TGATGGACGA CGAGAATTAC AATCCCGATC ACGGCATGGA TCTGACCTCT
    CAGGGGGCGG GAACCTACTG GTATCTGCCA CCCGAGTGCT TTGTCGTGGG CAAAAATCCG
    CCGAAAATCT CCTCCAAAGT GGACGTATGG AGTGTGGGTG TTATCTTCTA CCAGTGTCTG
    TACGGCAAAA AGCCCTTCGG TCACAATCAG TCGCAGGCCA CGATTCTCGA GGAGAATACG
    ATCCTGAAGG CCACCGAAGT GCAGTTCTCC AACAAGCCAA CCGTTTCTAA CGAGGCCAAG
    AGTTTCATTC GGGGATGCTT GGCCTATCGC AAGGAGGATC GCATGGATGT GTTCGCACTG
    GCCAGGCACG AGTACATTCA GCCACCGATA CCGAAACATG GGCGCGGTTC GCTCAATCAG
    CAACAGCAGG CGCAACAACA GCAGCAGCAA CAACAGCAAC AGCAGCAGCA ACAGTCGTCG
    ACGTCACAGG CCAATTCTAC AGGCCAGACA TCTTTCTCTG CCCACATGTT TGGCAATATG
    AATCAGTCGA GTTCGTCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02498
    Clone ID Related Accession (Same CDS sequence) NM_001144682.2 , NM_001169184.2 , NM_001144680.3
    Accession Version NM_001144680.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Tousled-like kinase, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001144680.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCAAT TTCAAACTTC ACTAAATCCG CGGAAACTCG AGCTTCTGGA GTCTCGAATT 
    ACTGGAGTGC GGATGTCCCC CGGCGCCCAT TTGCAGATGT CCCCGCAGAA CACTTCGTCC
    CTAAGTCAAC ACCATCCACA TCAACAGCAA CAGTTACAAC CCCCACAGCA GCAACAACAG
    CATTTCCCTA ACCATCACAG CGCCCAGCAA CAGTCGCAGC AGCAGCAGCA ACAGGAGCAA
    CAGAATCCCC AGCAGCAGGC GCAACAGCAG CAGCAGATAC TCCCACATCA ACATTTGCAG
    CACCTGCACA AGCATCCGCA TCAGCTGCAA CTGCATCAGC AGCAGCAACA ACAACTCCAC
    CAGCAACAGC AGCAACACTT CCACCAGCAG TCGCTGCAAG GGCTGCATCA GGGTAGCAGC
    AATCCGGATT CGAATATGAG CACTGGCTCC TCGCATAGCG AGAAGGATGT CAATGATATG
    CTGAGTGGCG GTGCAGCAAC GCCAGGAGCT GCAGCAGCAG CGATTCAACA GCAACATCCC
    GCCTTTGCGC CCACACTGGG AATGCAGCAA CCACCGCCGC CCCCACCTCA ACACTCCAAT
    AATGGAGGCG AGATGGGCTA CTTGTCGGCA GGCACGACCA CGACGACGTC GGTGTTAACG
    GTAGGCAAGC CTCGGACGCC AGCGGAGCGG AAACGGAAGC GAAAAATGCC TCCATGTGCC
    ACTAGTGCGG ATGAGGCGGG GAGTGGCGGT GGCTCTGGCG GAGCAGGAGC AACCGTTGTT
    AACAACAGCA GCCTGAAGGG CAAATCATTG GCCTTTCGTG ATATGCCCAA GGTAAACATG
    AGCCTGAATC TGGGCGATCG TCTGGGAGGA TCTGCAGGAA GCGGAGTAGG AGCCGGTGGC
    GCCGGAAGCG GGGGAGGTGG CGCTGGTTCC GGTTCTGGAA GCGGTGGCGG CAAAAGCGCC
    CGCCTGATGC TGCCAGTCAG CGACAACAAG AAGATCAACG ACTATTTCAA TAAGCAGCAA
    ACGGGCGTGG GCGTCGGTGT GCCAGGTGGT GCGGGAGGCA ATACCGCTGG CCTTCGAGGA
    TCACATACGG GAGGTGGCAG CAAGTCACCC TCATCCGCCC AGCAGCAGCA AACGGCGGCA
    CAGCAGCAGG GAAGCGGTGT TGCGACGGGA GGCAGTGCAG GCGGTTCCGC TGGCAACCAG
    GTGCAAGTGC AAACGAGCAG CGCTTACGCC CTTTACCCAC CAGCTAGTCC CCAAACCCAG
    ACGTCACAGC AACAGCAGCA GCAGCAACCG GGATCAGACT TTCACTATGT CAACTCCAGC
    AAGGCGCAGC AACAACAGCA GCGTCAACAG CAACAGACTT CCAATCAAAT GGTTCCTCCA
    CACGTGGTCG TTGGCCTTGG TGGTCATCCA CTGAGCCTCG CGTCCATTCA GCAGCAGACG
    CCCTTATCCC AGCAGCAACA GCAGCAACAA CAGCAGCAGC AACAGCAGCA ACTGGGACCA
    CCGACCACAT CGACGGCCTC CGTCGTGCCA ACGCATCCGC ATCAACTCGG ATCCCTGGGA
    GTTGTTGGGA TGGTCGGTGT GGGTGTTGGC GTGGGCGTTG GAGTAAATGT GGGTGTGGGA
    CCACCACTGC CACCACCACC GCCGATGGCC ATGCCAGCGG CCATTATCAC TTATAGTAAG
    GCCACTCAAA CGGAGGTGTC GCTGCATGAA TTGCAGGAGC GCGAAGCGGA GCACGAATCG
    GGCAAGGTGA AGCTAGACGA GATGACACGG CTGTCCGATG AACAAAAGTC CCAAATTGTT
    GGCAACCAGA AGACGATTGA CCAGCACAAG TGCCACATAG CCAAGTGTAT TGATGTGGTC
    AAGAAGCTGT TGAAGGAGAA GAGCAGCATC GAGAAGAAGG AGGCGCGACA GAAGTGCATG
    CAGAATCGCC TCAGGCTCGG ACAGTTTGTT ACCCAACGAG TGGGCGCCAC ATTCCAGGAG
    AACTGGACGG ACGGCTATGC GTTCCAGGAG CTGAGTCGGC GGCAAGAAGA AATAACCGCT
    GAGCGTGAAG AGATAGATCG GCAGAAAAAG CAGCTGATGA AAAAGCGTCC GGCGGAGTCC
    GGACGCAAGC GCAACAACAA CAGTAACCAG AACAACCAGC AGCAGCAGCA ACAGCAACAC
    CAGCAACAGC AGCAGCAACA AAATTCCAAC TCGAACGATT CCACGCAGCT GACGAGCGGA
    GTTGTTACCG GTCCAGGCAG TGATCGTGTG AGCGTAAGCG TCGACAGCGG ATTGGGTGGC
    AATAATGCGG GCGCGATCGG TGGCGGAACC GTTGGTGGTG GCGTTGGAGG TGGTGGTGTT
    GGAGGCGGTG GTGTCGGAGG CGGCGGTGGA CGTGGACTTT CTCGCAGCAA TTCGACGCAG
    GCCAATCAGG CTCAATTGCT GCACAACGGC GGTGGTGGTT CGGGCGGCAA TGTCGGCAAC
    TCGGGCGGCG TTGGCGACCG CTTGTCAGAT CGAGGAGGAG GAGGTGGCGG CATCGGCGGA
    AACGATAGCG GCAGCTGCTC GGACTCGGGC ACTTTCCTGA AGCCAGACCC CGTATCGGGT
    GCCTACACAG CGCAGGAGTA TTACGAGTAC GATGAGATCC TCAAGTTGCG ACAAAATGCC
    CTCAAAAAGG AGGACGCCGA CCTGCAGCTG GAGATGGAGA AGCTGGAGCG GGAGCGCAAT
    CTGCACATCC GAGAGCTCAA GCGGATTCTT AACGAGGATC AGTCCCGCTT TAACAATCAT
    CCCGTGCTGA ATGATCGCTA TCTTCTGTTG ATGCTCCTGG GCAAGGGCGG CTTCTCAGAG
    GTCCACAAGG CCTTCGACCT GAAGGAGCAA CGCTATGTCG CATGTAAGGT GCACCAATTA
    AACAAGGATT GGAAGGAGGA TAAGAAAGCT AATTATATCA AACACGCTTT GCGGGAATAC
    AACATTCACA AGGCACTGGA TCATCCGCGG GTCGTCAAGC TATACGATGT CTTCGAGATC
    GATGCGAATT CCTTTTGCAC AGTGCTCGAA TACTGTGATG GCCACGATCT GGACTTCTAT
    TTGAAGCAAC ATAAGACTAT ACCCGAGCGT GAAGCGCGCT CGATAATAAT GCAGGTTGTA
    TCTGCACTCA AGTATCTAAA TGAGATTAAG CCTCCAGTTA TCCACTACGA TCTGAAGCCC
    GGCAACATTC TGCTTACCGA GGGCAACGTC TGCGGCGAGA TTAAGATCAC CGACTTCGGT
    CTGTCAAAGG TGATGGACGA CGAGAATTAC AATCCCGATC ACGGCATGGA TCTGACCTCT
    CAGGGGGCGG GAACCTACTG GTATCTGCCA CCCGAGTGCT TTGTCGTGGG CAAAAATCCG
    CCGAAAATCT CCTCCAAAGT GGACGTATGG AGTGTGGGTG TTATCTTCTA CCAGTGTCTG
    TACGGCAAAA AGCCCTTCGG TCACAATCAG TCGCAGGCCA CGATTCTCGA GGAGAATACG
    ATCCTGAAGG CCACCGAAGT GCAGTTCTCC AACAAGCCAA CCGTTTCTAA CGAGGCCAAG
    AGTTTCATTC GGGGATGCTT GGCCTATCGC AAGGAGGATC GCATGGATGT GTTCGCACTG
    GCCAGGCACG AGTACATTCA GCCACCGATA CCGAAACATG GGCGCGGTTC GCTCAATCAG
    CAACAGCAGG CGCAACAACA GCAGCAGCAA CAACAGCAAC AGCAGCAGCA ACAGTCGTCG
    ACGTCACAGG CCAATTCTAC AGGCCAGACA TCTTTCTCTG CCCACATGTT TGGCAATATG
    AATCAGTCGA GTTCGTCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138152.1
    CDS299..4099
    Translation

    Target ORF information:

    RefSeq Version NM_001144680.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144680.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATCAAT TTCAAACTTC ACTAAATCCG CGGAAACTCG AGCTTCTGGA GTCTCGAATT 
    ACTGGAGTGC GGATGTCCCC CGGCGCCCAT TTGCAGATGT CCCCGCAGAA CACTTCGTCC
    CTAAGTCAAC ACCATCCACA TCAACAGCAA CAGTTACAAC CCCCACAGCA GCAACAACAG
    CATTTCCCTA ACCATCACAG CGCCCAGCAA CAGTCGCAGC AGCAGCAGCA ACAGGAGCAA
    CAGAATCCCC AGCAGCAGGC GCAACAGCAG CAGCAGATAC TCCCACATCA ACATTTGCAG
    CACCTGCACA AGCATCCGCA TCAGCTGCAA CTGCATCAGC AGCAGCAACA ACAACTCCAC
    CAGCAACAGC AGCAACACTT CCACCAGCAG TCGCTGCAAG GGCTGCATCA GGGTAGCAGC
    AATCCGGATT CGAATATGAG CACTGGCTCC TCGCATAGCG AGAAGGATGT CAATGATATG
    CTGAGTGGCG GTGCAGCAAC GCCAGGAGCT GCAGCAGCAG CGATTCAACA GCAACATCCC
    GCCTTTGCGC CCACACTGGG AATGCAGCAA CCACCGCCGC CCCCACCTCA ACACTCCAAT
    AATGGAGGCG AGATGGGCTA CTTGTCGGCA GGCACGACCA CGACGACGTC GGTGTTAACG
    GTAGGCAAGC CTCGGACGCC AGCGGAGCGG AAACGGAAGC GAAAAATGCC TCCATGTGCC
    ACTAGTGCGG ATGAGGCGGG GAGTGGCGGT GGCTCTGGCG GAGCAGGAGC AACCGTTGTT
    AACAACAGCA GCCTGAAGGG CAAATCATTG GCCTTTCGTG ATATGCCCAA GGTAAACATG
    AGCCTGAATC TGGGCGATCG TCTGGGAGGA TCTGCAGGAA GCGGAGTAGG AGCCGGTGGC
    GCCGGAAGCG GGGGAGGTGG CGCTGGTTCC GGTTCTGGAA GCGGTGGCGG CAAAAGCGCC
    CGCCTGATGC TGCCAGTCAG CGACAACAAG AAGATCAACG ACTATTTCAA TAAGCAGCAA
    ACGGGCGTGG GCGTCGGTGT GCCAGGTGGT GCGGGAGGCA ATACCGCTGG CCTTCGAGGA
    TCACATACGG GAGGTGGCAG CAAGTCACCC TCATCCGCCC AGCAGCAGCA AACGGCGGCA
    CAGCAGCAGG GAAGCGGTGT TGCGACGGGA GGCAGTGCAG GCGGTTCCGC TGGCAACCAG
    GTGCAAGTGC AAACGAGCAG CGCTTACGCC CTTTACCCAC CAGCTAGTCC CCAAACCCAG
    ACGTCACAGC AACAGCAGCA GCAGCAACCG GGATCAGACT TTCACTATGT CAACTCCAGC
    AAGGCGCAGC AACAACAGCA GCGTCAACAG CAACAGACTT CCAATCAAAT GGTTCCTCCA
    CACGTGGTCG TTGGCCTTGG TGGTCATCCA CTGAGCCTCG CGTCCATTCA GCAGCAGACG
    CCCTTATCCC AGCAGCAACA GCAGCAACAA CAGCAGCAGC AACAGCAGCA ACTGGGACCA
    CCGACCACAT CGACGGCCTC CGTCGTGCCA ACGCATCCGC ATCAACTCGG ATCCCTGGGA
    GTTGTTGGGA TGGTCGGTGT GGGTGTTGGC GTGGGCGTTG GAGTAAATGT GGGTGTGGGA
    CCACCACTGC CACCACCACC GCCGATGGCC ATGCCAGCGG CCATTATCAC TTATAGTAAG
    GCCACTCAAA CGGAGGTGTC GCTGCATGAA TTGCAGGAGC GCGAAGCGGA GCACGAATCG
    GGCAAGGTGA AGCTAGACGA GATGACACGG CTGTCCGATG AACAAAAGTC CCAAATTGTT
    GGCAACCAGA AGACGATTGA CCAGCACAAG TGCCACATAG CCAAGTGTAT TGATGTGGTC
    AAGAAGCTGT TGAAGGAGAA GAGCAGCATC GAGAAGAAGG AGGCGCGACA GAAGTGCATG
    CAGAATCGCC TCAGGCTCGG ACAGTTTGTT ACCCAACGAG TGGGCGCCAC ATTCCAGGAG
    AACTGGACGG ACGGCTATGC GTTCCAGGAG CTGAGTCGGC GGCAAGAAGA AATAACCGCT
    GAGCGTGAAG AGATAGATCG GCAGAAAAAG CAGCTGATGA AAAAGCGTCC GGCGGAGTCC
    GGACGCAAGC GCAACAACAA CAGTAACCAG AACAACCAGC AGCAGCAGCA ACAGCAACAC
    CAGCAACAGC AGCAGCAACA AAATTCCAAC TCGAACGATT CCACGCAGCT GACGAGCGGA
    GTTGTTACCG GTCCAGGCAG TGATCGTGTG AGCGTAAGCG TCGACAGCGG ATTGGGTGGC
    AATAATGCGG GCGCGATCGG TGGCGGAACC GTTGGTGGTG GCGTTGGAGG TGGTGGTGTT
    GGAGGCGGTG GTGTCGGAGG CGGCGGTGGA CGTGGACTTT CTCGCAGCAA TTCGACGCAG
    GCCAATCAGG CTCAATTGCT GCACAACGGC GGTGGTGGTT CGGGCGGCAA TGTCGGCAAC
    TCGGGCGGCG TTGGCGACCG CTTGTCAGAT CGAGGAGGAG GAGGTGGCGG CATCGGCGGA
    AACGATAGCG GCAGCTGCTC GGACTCGGGC ACTTTCCTGA AGCCAGACCC CGTATCGGGT
    GCCTACACAG CGCAGGAGTA TTACGAGTAC GATGAGATCC TCAAGTTGCG ACAAAATGCC
    CTCAAAAAGG AGGACGCCGA CCTGCAGCTG GAGATGGAGA AGCTGGAGCG GGAGCGCAAT
    CTGCACATCC GAGAGCTCAA GCGGATTCTT AACGAGGATC AGTCCCGCTT TAACAATCAT
    CCCGTGCTGA ATGATCGCTA TCTTCTGTTG ATGCTCCTGG GCAAGGGCGG CTTCTCAGAG
    GTCCACAAGG CCTTCGACCT GAAGGAGCAA CGCTATGTCG CATGTAAGGT GCACCAATTA
    AACAAGGATT GGAAGGAGGA TAAGAAAGCT AATTATATCA AACACGCTTT GCGGGAATAC
    AACATTCACA AGGCACTGGA TCATCCGCGG GTCGTCAAGC TATACGATGT CTTCGAGATC
    GATGCGAATT CCTTTTGCAC AGTGCTCGAA TACTGTGATG GCCACGATCT GGACTTCTAT
    TTGAAGCAAC ATAAGACTAT ACCCGAGCGT GAAGCGCGCT CGATAATAAT GCAGGTTGTA
    TCTGCACTCA AGTATCTAAA TGAGATTAAG CCTCCAGTTA TCCACTACGA TCTGAAGCCC
    GGCAACATTC TGCTTACCGA GGGCAACGTC TGCGGCGAGA TTAAGATCAC CGACTTCGGT
    CTGTCAAAGG TGATGGACGA CGAGAATTAC AATCCCGATC ACGGCATGGA TCTGACCTCT
    CAGGGGGCGG GAACCTACTG GTATCTGCCA CCCGAGTGCT TTGTCGTGGG CAAAAATCCG
    CCGAAAATCT CCTCCAAAGT GGACGTATGG AGTGTGGGTG TTATCTTCTA CCAGTGTCTG
    TACGGCAAAA AGCCCTTCGG TCACAATCAG TCGCAGGCCA CGATTCTCGA GGAGAATACG
    ATCCTGAAGG CCACCGAAGT GCAGTTCTCC AACAAGCCAA CCGTTTCTAA CGAGGCCAAG
    AGTTTCATTC GGGGATGCTT GGCCTATCGC AAGGAGGATC GCATGGATGT GTTCGCACTG
    GCCAGGCACG AGTACATTCA GCCACCGATA CCGAAACATG GGCGCGGTTC GCTCAATCAG
    CAACAGCAGG CGCAACAACA GCAGCAGCAA CAACAGCAAC AGCAGCAGCA ACAGTCGTCG
    ACGTCACAGG CCAATTCTAC AGGCCAGACA TCTTTCTCTG CCCACATGTT TGGCAATATG
    AATCAGTCGA GTTCGTCCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02499
    Clone ID Related Accession (Same CDS sequence) NM_001272291.1
    Accession Version NM_001272291.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Tousled-like kinase, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGCAGCA AACGAAATAC CAGCGATTTG GGCCTATTGC AGCGCACCTG CATTGTCGGG 
    GGAAAATCGA TGTCCCCCGG CGCCCATTTG CAGATGTCCC CGCAGAACAC TTCGTCCCTA
    AGTCAACACC ATCCACATCA ACAGCAACAG TTACAACCCC CACAGCAGCA ACAACAGCAT
    TTCCCTAACC ATCACAGCGC CCAGCAACAG TCGCAGCAGC AGCAGCAACA GGAGCAACAG
    AATCCCCAGC AGCAGGCGCA ACAGCAGCAG CAGATACTCC CACATCAACA TTTGCAGCAC
    CTGCACAAGC ATCCGCATCA GCTGCAACTG CATCAGCAGC AGCAACAACA ACTCCACCAG
    CAACAGCAGC AACACTTCCA CCAGCAGTCG CTGCAAGGGC TGCATCAGGG TAGCAGCAAT
    CCGGATTCGA ATATGAGCAC TGGCTCCTCG CATAGCGAGA AGGATGTCAA TGATATGCTG
    AGTGGCGGTG CAGCAACGCC AGGAGCTGCA GCAGCAGCGA TTCAACAGCA ACATCCCGCC
    TTTGCGCCCA CACTGGGAAT GCAGCAACCA CCGCCGCCCC CACCTCAACA CTCCAATAAT
    GGAGGCGAGA TGGGCTACTT GTCGGCAGGC ACGACCACGA CGACGTCGGT GTTAACGGTA
    GGCAAGCCTC GGACGCCAGC GGAGCGGAAA CGGAAGCGAA AAATGCCTCC ATGTGCCACT
    AGTGCGGATG AGGCGGGGAG TGGCGGTGGC TCTGGCGGAG CAGGAGCAAC CGTTGTTAAC
    AACAGCAGCC TGAAGGGCAA ATCATTGGCC TTTCGTGATA TGCCCAAGGT AAACATGAGC
    CTGAATCTGG GCGATCGTCT GGGAGGATCT GCAGGAAGCG GAGTAGGAGC CGGTGGCGCC
    GGAAGCGGGG GAGGTGGCGC TGGTTCCGGT TCTGGAAGCG GTGGCGGCAA AAGCGCCCGC
    CTGATGCTGC CAGTCAGCGA CAACAAGAAG ATCAACGACT ATTTCAATAA GCAGCAAACG
    GGCGTGGGCG TCGGTGTGCC AGGTGGTGCG GGAGGCAATA CCGCTGGCCT TCGAGGATCA
    CATACGGGAG GTGGCAGCAA GTCACCCTCA TCCGCCCAGC AGCAGCAAAC GGCGGCACAG
    CAGCAGGGAA GCGGTGTTGC GACGGGAGGC AGTGCAGGCG GTTCCGCTGG CAACCAGGTG
    CAAGTGCAAA CGAGCAGCGC TTACGCCCTT TACCCACCAG CTAGTCCCCA AACCCAGACG
    TCACAGCAAC AGCAGCAGCA GCAACCGGGA TCAGACTTTC ACTATGTCAA CTCCAGCAAG
    GCGCAGCAAC AACAGCAGCG TCAACAGCAA CAGACTTCCA ATCAAATGGT TCCTCCACAC
    GTGGTCGTTG GCCTTGGTGG TCATCCACTG AGCCTCGCGT CCATTCAGCA GCAGACGCCC
    TTATCCCAGC AGCAACAGCA GCAACAACAG CAGCAGCAAC AGCAGCAACT GGGACCACCG
    ACCACATCGA CGGCCTCCGT CGTGCCAACG CATCCGCATC AACTCGGATC CCTGGGAGTT
    GTTGGGATGG TCGGTGTGGG TGTTGGCGTG GGCGTTGGAG TAAATGTGGG TGTGGGACCA
    CCACTGCCAC CACCACCGCC GATGGCCATG CCAGCGGCCA TTATCACTTA TAGTAAGGCC
    ACTCAAACGG AGGTGTCGCT GCATGAATTG CAGGAGCGCG AAGCGGAGCA CGAATCGGGC
    AAGGTGAAGC TAGACGAGAT GACACGGCTG TCCGATGAAC AAAAGTCCCA AATTGTTGGC
    AACCAGAAGA CGATTGACCA GCACAAGTGC CACATAGCCA AGTGTATTGA TGTGGTCAAG
    AAGCTGTTGA AGGAGAAGAG CAGCATCGAG AAGAAGGAGG CGCGACAGAA GTGCATGCAG
    AATCGCCTCA GGCTCGGACA GTTTGTTACC CAACGAGTGG GCGCCACATT CCAGGAGAAC
    TGGACGGACG GCTATGCGTT CCAGGAGCTG AGTCGGCGGC AAGAAGAAAT AACCGCTGAG
    CGTGAAGAGA TAGATCGGCA GAAAAAGCAG CTGATGAAAA AGCGTCCGGC GGAGTCCGGA
    CGCAAGCGCA ACAACAACAG TAACCAGAAC AACCAGCAGC AGCAGCAACA GCAACACCAG
    CAACAGCAGC AGCAACAAAA TTCCAACTCG AACGATTCCA CGCAGCTGAC GAGCGGAGTT
    GTTACCGGTC CAGGCAGTGA TCGTGTGAGC GTAAGCGTCG ACAGCGGATT GGGTGGCAAT
    AATGCGGGCG CGATCGGTGG CGGAACCGTT GGTGGTGGCG TTGGAGGTGG TGGTGTTGGA
    GGCGGTGGTG TCGGAGGCGG CGGTGGACGT GGACTTTCTC GCAGCAATTC GACGCAGGCC
    AATCAGGCTC AATTGCTGCA CAACGGCGGT GGTGGTTCGG GCGGCAATGT CGGCAACTCG
    GGCGGCGTTG GCGACCGCTT GTCAGATCGA GGAGGAGGAG GTGGCGGCAT CGGCGGAAAC
    GATAGCGGCA GCTGCTCGGA CTCGGGCACT TTCCTGAAGC CAGACCCCGT ATCGGGTGCC
    TACACAGCGC AGGAGTATTA CGAGTACGAT GAGATCCTCA AGTTGCGACA AAATGCCCTC
    AAAAAGGAGG ACGCCGACCT GCAGCTGGAG ATGGAGAAGC TGGAGCGGGA GCGCAATCTG
    CACATCCGAG AGCTCAAGCG GATTCTTAAC GAGGATCAGT CCCGCTTTAA CAATCATCCC
    GTGCTGAATG ATCGCTATCT TCTGTTGATG CTCCTGGGCA AGGGCGGCTT CTCAGAGGTC
    CACAAGGCCT TCGACCTGAA GGAGCAACGC TATGTCGCAT GTAAGGTGCA CCAATTAAAC
    AAGGATTGGA AGGAGGATAA GAAAGCTAAT TATATCAAAC ACGCTTTGCG GGAATACAAC
    ATTCACAAGG CACTGGATCA TCCGCGGGTC GTCAAGCTAT ACGATGTCTT CGAGATCGAT
    GCGAATTCCT TTTGCACAGT GCTCGAATAC TGTGATGGCC ACGATCTGGA CTTCTATTTG
    AAGCAACATA AGACTATACC CGAGCGTGAA GCGCGCTCGA TAATAATGCA GGTTGTATCT
    GCACTCAAGT ATCTAAATGA GATTAAGCCT CCAGTTATCC ACTACGATCT GAAGCCCGGC
    AACATTCTGC TTACCGAGGG CAACGTCTGC GGCGAGATTA AGATCACCGA CTTCGGTCTG
    TCAAAGGTGA TGGACGACGA GAATTACAAT CCCGATCACG GCATGGATCT GACCTCTCAG
    GGGGCGGGAA CCTACTGGTA TCTGCCACCC GAGTGCTTTG TCGTGGGCAA AAATCCGCCG
    AAAATCTCCT CCAAAGTGGA CGTATGGAGT GTGGGTGTTA TCTTCTACCA GTGTCTGTAC
    GGCAAAAAGC CCTTCGGTCA CAATCAGTCG CAGGCCACGA TTCTCGAGGA GAATACGATC
    CTGAAGGCCA CCGAAGTGCA GTTCTCCAAC AAGCCAACCG TTTCTAACGA GGCCAAGAGT
    TTCATTCGGG GATGCTTGGC CTATCGCAAG GAGGATCGCA TGGATGTGTT CGCACTGGCC
    AGGCACGAGT ACATTCAGCC ACCGATACCG AAACATGGGC GCGGTTCGCT CAATCAGCAA
    CAGCAGGCGC AACAACAGCA GCAGCAACAA CAGCAACAGC AGCAGCAACA GTCGTCGACG
    TCACAGGCCA ATTCTACAGG CCAGACATCT TTCTCTGCCC ACATGTTTGG CAATATGAAT
    CAGTCGAGTT CGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259220.1
    CDS240..4037
    Translation

    Target ORF information:

    RefSeq Version NM_001272291.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272291.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGCAGCA AACGAAATAC CAGCGATTTG GGCCTATTGC AGCGCACCTG CATTGTCGGG 
    GGAAAATCGA TGTCCCCCGG CGCCCATTTG CAGATGTCCC CGCAGAACAC TTCGTCCCTA
    AGTCAACACC ATCCACATCA ACAGCAACAG TTACAACCCC CACAGCAGCA ACAACAGCAT
    TTCCCTAACC ATCACAGCGC CCAGCAACAG TCGCAGCAGC AGCAGCAACA GGAGCAACAG
    AATCCCCAGC AGCAGGCGCA ACAGCAGCAG CAGATACTCC CACATCAACA TTTGCAGCAC
    CTGCACAAGC ATCCGCATCA GCTGCAACTG CATCAGCAGC AGCAACAACA ACTCCACCAG
    CAACAGCAGC AACACTTCCA CCAGCAGTCG CTGCAAGGGC TGCATCAGGG TAGCAGCAAT
    CCGGATTCGA ATATGAGCAC TGGCTCCTCG CATAGCGAGA AGGATGTCAA TGATATGCTG
    AGTGGCGGTG CAGCAACGCC AGGAGCTGCA GCAGCAGCGA TTCAACAGCA ACATCCCGCC
    TTTGCGCCCA CACTGGGAAT GCAGCAACCA CCGCCGCCCC CACCTCAACA CTCCAATAAT
    GGAGGCGAGA TGGGCTACTT GTCGGCAGGC ACGACCACGA CGACGTCGGT GTTAACGGTA
    GGCAAGCCTC GGACGCCAGC GGAGCGGAAA CGGAAGCGAA AAATGCCTCC ATGTGCCACT
    AGTGCGGATG AGGCGGGGAG TGGCGGTGGC TCTGGCGGAG CAGGAGCAAC CGTTGTTAAC
    AACAGCAGCC TGAAGGGCAA ATCATTGGCC TTTCGTGATA TGCCCAAGGT AAACATGAGC
    CTGAATCTGG GCGATCGTCT GGGAGGATCT GCAGGAAGCG GAGTAGGAGC CGGTGGCGCC
    GGAAGCGGGG GAGGTGGCGC TGGTTCCGGT TCTGGAAGCG GTGGCGGCAA AAGCGCCCGC
    CTGATGCTGC CAGTCAGCGA CAACAAGAAG ATCAACGACT ATTTCAATAA GCAGCAAACG
    GGCGTGGGCG TCGGTGTGCC AGGTGGTGCG GGAGGCAATA CCGCTGGCCT TCGAGGATCA
    CATACGGGAG GTGGCAGCAA GTCACCCTCA TCCGCCCAGC AGCAGCAAAC GGCGGCACAG
    CAGCAGGGAA GCGGTGTTGC GACGGGAGGC AGTGCAGGCG GTTCCGCTGG CAACCAGGTG
    CAAGTGCAAA CGAGCAGCGC TTACGCCCTT TACCCACCAG CTAGTCCCCA AACCCAGACG
    TCACAGCAAC AGCAGCAGCA GCAACCGGGA TCAGACTTTC ACTATGTCAA CTCCAGCAAG
    GCGCAGCAAC AACAGCAGCG TCAACAGCAA CAGACTTCCA ATCAAATGGT TCCTCCACAC
    GTGGTCGTTG GCCTTGGTGG TCATCCACTG AGCCTCGCGT CCATTCAGCA GCAGACGCCC
    TTATCCCAGC AGCAACAGCA GCAACAACAG CAGCAGCAAC AGCAGCAACT GGGACCACCG
    ACCACATCGA CGGCCTCCGT CGTGCCAACG CATCCGCATC AACTCGGATC CCTGGGAGTT
    GTTGGGATGG TCGGTGTGGG TGTTGGCGTG GGCGTTGGAG TAAATGTGGG TGTGGGACCA
    CCACTGCCAC CACCACCGCC GATGGCCATG CCAGCGGCCA TTATCACTTA TAGTAAGGCC
    ACTCAAACGG AGGTGTCGCT GCATGAATTG CAGGAGCGCG AAGCGGAGCA CGAATCGGGC
    AAGGTGAAGC TAGACGAGAT GACACGGCTG TCCGATGAAC AAAAGTCCCA AATTGTTGGC
    AACCAGAAGA CGATTGACCA GCACAAGTGC CACATAGCCA AGTGTATTGA TGTGGTCAAG
    AAGCTGTTGA AGGAGAAGAG CAGCATCGAG AAGAAGGAGG CGCGACAGAA GTGCATGCAG
    AATCGCCTCA GGCTCGGACA GTTTGTTACC CAACGAGTGG GCGCCACATT CCAGGAGAAC
    TGGACGGACG GCTATGCGTT CCAGGAGCTG AGTCGGCGGC AAGAAGAAAT AACCGCTGAG
    CGTGAAGAGA TAGATCGGCA GAAAAAGCAG CTGATGAAAA AGCGTCCGGC GGAGTCCGGA
    CGCAAGCGCA ACAACAACAG TAACCAGAAC AACCAGCAGC AGCAGCAACA GCAACACCAG
    CAACAGCAGC AGCAACAAAA TTCCAACTCG AACGATTCCA CGCAGCTGAC GAGCGGAGTT
    GTTACCGGTC CAGGCAGTGA TCGTGTGAGC GTAAGCGTCG ACAGCGGATT GGGTGGCAAT
    AATGCGGGCG CGATCGGTGG CGGAACCGTT GGTGGTGGCG TTGGAGGTGG TGGTGTTGGA
    GGCGGTGGTG TCGGAGGCGG CGGTGGACGT GGACTTTCTC GCAGCAATTC GACGCAGGCC
    AATCAGGCTC AATTGCTGCA CAACGGCGGT GGTGGTTCGG GCGGCAATGT CGGCAACTCG
    GGCGGCGTTG GCGACCGCTT GTCAGATCGA GGAGGAGGAG GTGGCGGCAT CGGCGGAAAC
    GATAGCGGCA GCTGCTCGGA CTCGGGCACT TTCCTGAAGC CAGACCCCGT ATCGGGTGCC
    TACACAGCGC AGGAGTATTA CGAGTACGAT GAGATCCTCA AGTTGCGACA AAATGCCCTC
    AAAAAGGAGG ACGCCGACCT GCAGCTGGAG ATGGAGAAGC TGGAGCGGGA GCGCAATCTG
    CACATCCGAG AGCTCAAGCG GATTCTTAAC GAGGATCAGT CCCGCTTTAA CAATCATCCC
    GTGCTGAATG ATCGCTATCT TCTGTTGATG CTCCTGGGCA AGGGCGGCTT CTCAGAGGTC
    CACAAGGCCT TCGACCTGAA GGAGCAACGC TATGTCGCAT GTAAGGTGCA CCAATTAAAC
    AAGGATTGGA AGGAGGATAA GAAAGCTAAT TATATCAAAC ACGCTTTGCG GGAATACAAC
    ATTCACAAGG CACTGGATCA TCCGCGGGTC GTCAAGCTAT ACGATGTCTT CGAGATCGAT
    GCGAATTCCT TTTGCACAGT GCTCGAATAC TGTGATGGCC ACGATCTGGA CTTCTATTTG
    AAGCAACATA AGACTATACC CGAGCGTGAA GCGCGCTCGA TAATAATGCA GGTTGTATCT
    GCACTCAAGT ATCTAAATGA GATTAAGCCT CCAGTTATCC ACTACGATCT GAAGCCCGGC
    AACATTCTGC TTACCGAGGG CAACGTCTGC GGCGAGATTA AGATCACCGA CTTCGGTCTG
    TCAAAGGTGA TGGACGACGA GAATTACAAT CCCGATCACG GCATGGATCT GACCTCTCAG
    GGGGCGGGAA CCTACTGGTA TCTGCCACCC GAGTGCTTTG TCGTGGGCAA AAATCCGCCG
    AAAATCTCCT CCAAAGTGGA CGTATGGAGT GTGGGTGTTA TCTTCTACCA GTGTCTGTAC
    GGCAAAAAGC CCTTCGGTCA CAATCAGTCG CAGGCCACGA TTCTCGAGGA GAATACGATC
    CTGAAGGCCA CCGAAGTGCA GTTCTCCAAC AAGCCAACCG TTTCTAACGA GGCCAAGAGT
    TTCATTCGGG GATGCTTGGC CTATCGCAAG GAGGATCGCA TGGATGTGTT CGCACTGGCC
    AGGCACGAGT ACATTCAGCC ACCGATACCG AAACATGGGC GCGGTTCGCT CAATCAGCAA
    CAGCAGGCGC AACAACAGCA GCAGCAACAA CAGCAACAGC AGCAGCAACA GTCGTCGACG
    TCACAGGCCA ATTCTACAGG CCAGACATCT TTCTCTGCCC ACATGTTTGG CAATATGAAT
    CAGTCGAGTT CGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02500
    Clone ID Related Accession (Same CDS sequence) NM_001297914.1 , NM_001144683.1 , NM_001272293.1
    Accession Version NM_001297914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Tousled-like kinase, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCCCCG GCGCCCATTT GCAGATGTCC CCGCAGAACA CTTCGTCCCT AAGTCAACAC 
    CATCCACATC AACAGCAACA GTTACAACCC CCACAGCAGC AACAACAGCA TTTCCCTAAC
    CATCACAGCG CCCAGCAACA GTCGCAGCAG CAGCAGCAAC AGGAGCAACA GAATCCCCAG
    CAGCAGGCGC AACAGCAGCA GCAGATACTC CCACATCAAC ATTTGCAGCA CCTGCACAAG
    CATCCGCATC AGCTGCAACT GCATCAGCAG CAGCAACAAC AACTCCACCA GCAACAGCAG
    CAACACTTCC ACCAGCAGTC GCTGCAAGGG CTGCATCAGG GTAGCAGCAA TCCGGATTCG
    AATATGAGCA CTGGCTCCTC GCATAGCGAG AAGGATGTCA ATGATATGCT GAGTGGCGGT
    GCAGCAACGC CAGGAGCTGC AGCAGCAGCG ATTCAACAGC AACATCCCGC CTTTGCGCCC
    ACACTGGGAA TGCAGCAACC ACCGCCGCCC CCACCTCAAC ACTCCAATAA TGGAGGCGAG
    ATGGGCTACT TGTCGGCAGG CACGACCACG ACGACGTCGG TGTTAACGGT AGGCAAGCCT
    CGGACGCCAG CGGAGCGGAA ACGGAAGCGA AAAATGCCTC CATGTGCCAC TAGTGCGGAT
    GAGGCGGGGA GTGGCGGTGG CTCTGGCGGA GCAGGAGCAA CCGTTGTTAA CAACAGCAGC
    CTGAAGGGCA AATCATTGGC CTTTCGTGAT ATGCCCAAGG TAAACATGAG CCTGAATCTG
    GGCGATCGTC TGGGAGGATC TGCAGGAAGC GGAGTAGGAG CCGGTGGCGC CGGAAGCGGG
    GGAGGTGGCG CTGGTTCCGG TTCTGGAAGC GGTGGCGGCA AAAGCGCCCG CCTGATGCTG
    CCAGTCAGCG ACAACAAGAA GATCAACGAC TATTTCAATA AGCAGCAAAC GGGCGTGGGC
    GTCGGTGTGC CAGGTGGTGC GGGAGGCAAT ACCGCTGGCC TTCGAGGATC ACATACGGGA
    GGTGGCAGCA AGTCACCCTC ATCCGCCCAG CAGCAGCAAA CGGCGGCACA GCAGCAGGGA
    AGCGGTGTTG CGACGGGAGG CAGTGCAGGC GGTTCCGCTG GCAACCAGGT GCAAGTGCAA
    ACGAGCAGCG CTTACGCCCT TTACCCACCA GCTAGTCCCC AAACCCAGAC GTCACAGCAA
    CAGCAGCAGC AGCAACCGGG ATCAGACTTT CACTATGTCA ACTCCAGCAA GGCGCAGCAA
    CAACAGCAGC GTCAACAGCA ACAGACTTCC AATCAAATGG TTCCTCCACA CGTGGTCGTT
    GGCCTTGGTG GTCATCCACT GAGCCTCGCG TCCATTCAGC AGCAGACGCC CTTATCCCAG
    CAGCAACAGC AGCAACAACA GCAGCAGCAA CAGCAGCAAC TGGGACCACC GACCACATCG
    ACGGCCTCCG TCGTGCCAAC GCATCCGCAT CAACTCGGAT CCCTGGGAGT TGTTGGGATG
    GTCGGTGTGG GTGTTGGCGT GGGCGTTGGA GTAAATGTGG GTGTGGGACC ACCACTGCCA
    CCACCACCGC CGATGGCCAT GCCAGCGGCC ATTATCACTT ATAGTAAGGC CACTCAAACG
    GAGGTGTCGC TGCATGAATT GCAGGAGCGC GAAGCGGAGC ACGAATCGGG CAAGGTGAAG
    CTAGACGAGA TGACACGGCT GTCCGATGAA CAAAAGTCCC AAATTGTTGG CAACCAGAAG
    ACGATTGACC AGCACAAGTG CCACATAGCC AAGTGTATTG ATGTGGTCAA GAAGCTGTTG
    AAGGAGAAGA GCAGCATCGA GAAGAAGGAG GCGCGACAGA AGTGCATGCA GAATCGCCTC
    AGGCTCGGAC AGTTTGTTAC CCAACGAGTG GGCGCCACAT TCCAGGAGAA CTGGACGGAC
    GGCTATGCGT TCCAGGAGCT GAGTCGGCGG CAAGAAGAAA TAACCGCTGA GCGTGAAGAG
    ATAGATCGGC AGAAAAAGCA GCTGATGAAA AAGCGTCCGG CGGAGTCCGG ACGCAAGCGC
    AACAACAACA GTAACCAGAA CAACCAGCAG CAGCAGCAAC AGCAACACCA GCAACAGCAG
    CAGCAACAAA ATTCCAACTC GAACGATTCC ACGCAGCTGA CGAGCGGAGT TGTTACCGGT
    CCAGGCAGTG ATCGTGTGAG CGTAAGCGTC GACAGCGGAT TGGGTGGCAA TAATGCGGGC
    GCGATCGGTG GCGGAACCGT TGGTGGTGGC GTTGGAGGTG GTGGTGTTGG AGGCGGTGGT
    GTCGGAGGCG GCGGTGGACG TGGACTTTCT CGCAGCAATT CGACGCAGGC CAATCAGGCT
    CAATTGCTGC ACAACGGCGG TGGTGGTTCG GGCGGCAATG TCGGCAACTC GGGCGGCGTT
    GGCGACCGCT TGTCAGATCG AGGAGGAGGA GGTGGCGGCA TCGGCGGAAA CGATAGCGGC
    AGCTGCTCGG ACTCGGGCAC TTTCCTGAAG CCAGACCCCG TATCGGGTGC CTACACAGCG
    CAGGAGTATT ACGAGTACGA TGAGATCCTC AAGTTGCGAC AAAATGCCCT CAAAAAGGAG
    GACGCCGACC TGCAGCTGGA GATGGAGAAG CTGGAGCGGG AGCGCAATCT GCACATCCGA
    GAGCTCAAGC GGATTCTTAA CGAGGATCAG TCCCGCTTTA ACAATCATCC CGTGCTGAAT
    GATCGCTATC TTCTGTTGAT GCTCCTGGGC AAGGGCGGCT TCTCAGAGGT CCACAAGGCC
    TTCGACCTGA AGGAGCAACG CTATGTCGCA TGTAAGGTGC ACCAATTAAA CAAGGATTGG
    AAGGAGGATA AGAAAGCTAA TTATATCAAA CACGCTTTGC GGGAATACAA CATTCACAAG
    GCACTGGATC ATCCGCGGGT CGTCAAGCTA TACGATGTCT TCGAGATCGA TGCGAATTCC
    TTTTGCACAG TGCTCGAATA CTGTGATGGC CACGATCTGG ACTTCTATTT GAAGCAACAT
    AAGACTATAC CCGAGCGTGA AGCGCGCTCG ATAATAATGC AGGTTGTATC TGCACTCAAG
    TATCTAAATG AGATTAAGCC TCCAGTTATC CACTACGATC TGAAGCCCGG CAACATTCTG
    CTTACCGAGG GCAACGTCTG CGGCGAGATT AAGATCACCG ACTTCGGTCT GTCAAAGGTG
    ATGGACGACG AGAATTACAA TCCCGATCAC GGCATGGATC TGACCTCTCA GGGGGCGGGA
    ACCTACTGGT ATCTGCCACC CGAGTGCTTT GTCGTGGGCA AAAATCCGCC GAAAATCTCC
    TCCAAAGTGG ACGTATGGAG TGTGGGTGTT ATCTTCTACC AGTGTCTGTA CGGCAAAAAG
    CCCTTCGGTC ACAATCAGTC GCAGGCCACG ATTCTCGAGG AGAATACGAT CCTGAAGGCC
    ACCGAAGTGC AGTTCTCCAA CAAGCCAACC GTTTCTAACG AGGCCAAGAG TTTCATTCGG
    GGATGCTTGG CCTATCGCAA GGAGGATCGC ATGGATGTGT TCGCACTGGC CAGGCACGAG
    TACATTCAGC CACCGATACC GAAACATGGG CGCGGTTCGC TCAATCAGCA ACAGCAGGCG
    CAACAACAGC AGCAGCAACA ACAGCAACAG CAGCAGCAAC AGTCGTCGAC GTCACAGGCC
    AATTCTACAG GCCAGACATC TTTCTCTGCC CACATGTTTG GCAATATGAA TCAGTCGAGT
    TCGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001284843.1
    CDS395..4123
    Translation

    Target ORF information:

    RefSeq Version NM_001297914.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant N, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001297914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCCCCG GCGCCCATTT GCAGATGTCC CCGCAGAACA CTTCGTCCCT AAGTCAACAC 
    CATCCACATC AACAGCAACA GTTACAACCC CCACAGCAGC AACAACAGCA TTTCCCTAAC
    CATCACAGCG CCCAGCAACA GTCGCAGCAG CAGCAGCAAC AGGAGCAACA GAATCCCCAG
    CAGCAGGCGC AACAGCAGCA GCAGATACTC CCACATCAAC ATTTGCAGCA CCTGCACAAG
    CATCCGCATC AGCTGCAACT GCATCAGCAG CAGCAACAAC AACTCCACCA GCAACAGCAG
    CAACACTTCC ACCAGCAGTC GCTGCAAGGG CTGCATCAGG GTAGCAGCAA TCCGGATTCG
    AATATGAGCA CTGGCTCCTC GCATAGCGAG AAGGATGTCA ATGATATGCT GAGTGGCGGT
    GCAGCAACGC CAGGAGCTGC AGCAGCAGCG ATTCAACAGC AACATCCCGC CTTTGCGCCC
    ACACTGGGAA TGCAGCAACC ACCGCCGCCC CCACCTCAAC ACTCCAATAA TGGAGGCGAG
    ATGGGCTACT TGTCGGCAGG CACGACCACG ACGACGTCGG TGTTAACGGT AGGCAAGCCT
    CGGACGCCAG CGGAGCGGAA ACGGAAGCGA AAAATGCCTC CATGTGCCAC TAGTGCGGAT
    GAGGCGGGGA GTGGCGGTGG CTCTGGCGGA GCAGGAGCAA CCGTTGTTAA CAACAGCAGC
    CTGAAGGGCA AATCATTGGC CTTTCGTGAT ATGCCCAAGG TAAACATGAG CCTGAATCTG
    GGCGATCGTC TGGGAGGATC TGCAGGAAGC GGAGTAGGAG CCGGTGGCGC CGGAAGCGGG
    GGAGGTGGCG CTGGTTCCGG TTCTGGAAGC GGTGGCGGCA AAAGCGCCCG CCTGATGCTG
    CCAGTCAGCG ACAACAAGAA GATCAACGAC TATTTCAATA AGCAGCAAAC GGGCGTGGGC
    GTCGGTGTGC CAGGTGGTGC GGGAGGCAAT ACCGCTGGCC TTCGAGGATC ACATACGGGA
    GGTGGCAGCA AGTCACCCTC ATCCGCCCAG CAGCAGCAAA CGGCGGCACA GCAGCAGGGA
    AGCGGTGTTG CGACGGGAGG CAGTGCAGGC GGTTCCGCTG GCAACCAGGT GCAAGTGCAA
    ACGAGCAGCG CTTACGCCCT TTACCCACCA GCTAGTCCCC AAACCCAGAC GTCACAGCAA
    CAGCAGCAGC AGCAACCGGG ATCAGACTTT CACTATGTCA ACTCCAGCAA GGCGCAGCAA
    CAACAGCAGC GTCAACAGCA ACAGACTTCC AATCAAATGG TTCCTCCACA CGTGGTCGTT
    GGCCTTGGTG GTCATCCACT GAGCCTCGCG TCCATTCAGC AGCAGACGCC CTTATCCCAG
    CAGCAACAGC AGCAACAACA GCAGCAGCAA CAGCAGCAAC TGGGACCACC GACCACATCG
    ACGGCCTCCG TCGTGCCAAC GCATCCGCAT CAACTCGGAT CCCTGGGAGT TGTTGGGATG
    GTCGGTGTGG GTGTTGGCGT GGGCGTTGGA GTAAATGTGG GTGTGGGACC ACCACTGCCA
    CCACCACCGC CGATGGCCAT GCCAGCGGCC ATTATCACTT ATAGTAAGGC CACTCAAACG
    GAGGTGTCGC TGCATGAATT GCAGGAGCGC GAAGCGGAGC ACGAATCGGG CAAGGTGAAG
    CTAGACGAGA TGACACGGCT GTCCGATGAA CAAAAGTCCC AAATTGTTGG CAACCAGAAG
    ACGATTGACC AGCACAAGTG CCACATAGCC AAGTGTATTG ATGTGGTCAA GAAGCTGTTG
    AAGGAGAAGA GCAGCATCGA GAAGAAGGAG GCGCGACAGA AGTGCATGCA GAATCGCCTC
    AGGCTCGGAC AGTTTGTTAC CCAACGAGTG GGCGCCACAT TCCAGGAGAA CTGGACGGAC
    GGCTATGCGT TCCAGGAGCT GAGTCGGCGG CAAGAAGAAA TAACCGCTGA GCGTGAAGAG
    ATAGATCGGC AGAAAAAGCA GCTGATGAAA AAGCGTCCGG CGGAGTCCGG ACGCAAGCGC
    AACAACAACA GTAACCAGAA CAACCAGCAG CAGCAGCAAC AGCAACACCA GCAACAGCAG
    CAGCAACAAA ATTCCAACTC GAACGATTCC ACGCAGCTGA CGAGCGGAGT TGTTACCGGT
    CCAGGCAGTG ATCGTGTGAG CGTAAGCGTC GACAGCGGAT TGGGTGGCAA TAATGCGGGC
    GCGATCGGTG GCGGAACCGT TGGTGGTGGC GTTGGAGGTG GTGGTGTTGG AGGCGGTGGT
    GTCGGAGGCG GCGGTGGACG TGGACTTTCT CGCAGCAATT CGACGCAGGC CAATCAGGCT
    CAATTGCTGC ACAACGGCGG TGGTGGTTCG GGCGGCAATG TCGGCAACTC GGGCGGCGTT
    GGCGACCGCT TGTCAGATCG AGGAGGAGGA GGTGGCGGCA TCGGCGGAAA CGATAGCGGC
    AGCTGCTCGG ACTCGGGCAC TTTCCTGAAG CCAGACCCCG TATCGGGTGC CTACACAGCG
    CAGGAGTATT ACGAGTACGA TGAGATCCTC AAGTTGCGAC AAAATGCCCT CAAAAAGGAG
    GACGCCGACC TGCAGCTGGA GATGGAGAAG CTGGAGCGGG AGCGCAATCT GCACATCCGA
    GAGCTCAAGC GGATTCTTAA CGAGGATCAG TCCCGCTTTA ACAATCATCC CGTGCTGAAT
    GATCGCTATC TTCTGTTGAT GCTCCTGGGC AAGGGCGGCT TCTCAGAGGT CCACAAGGCC
    TTCGACCTGA AGGAGCAACG CTATGTCGCA TGTAAGGTGC ACCAATTAAA CAAGGATTGG
    AAGGAGGATA AGAAAGCTAA TTATATCAAA CACGCTTTGC GGGAATACAA CATTCACAAG
    GCACTGGATC ATCCGCGGGT CGTCAAGCTA TACGATGTCT TCGAGATCGA TGCGAATTCC
    TTTTGCACAG TGCTCGAATA CTGTGATGGC CACGATCTGG ACTTCTATTT GAAGCAACAT
    AAGACTATAC CCGAGCGTGA AGCGCGCTCG ATAATAATGC AGGTTGTATC TGCACTCAAG
    TATCTAAATG AGATTAAGCC TCCAGTTATC CACTACGATC TGAAGCCCGG CAACATTCTG
    CTTACCGAGG GCAACGTCTG CGGCGAGATT AAGATCACCG ACTTCGGTCT GTCAAAGGTG
    ATGGACGACG AGAATTACAA TCCCGATCAC GGCATGGATC TGACCTCTCA GGGGGCGGGA
    ACCTACTGGT ATCTGCCACC CGAGTGCTTT GTCGTGGGCA AAAATCCGCC GAAAATCTCC
    TCCAAAGTGG ACGTATGGAG TGTGGGTGTT ATCTTCTACC AGTGTCTGTA CGGCAAAAAG
    CCCTTCGGTC ACAATCAGTC GCAGGCCACG ATTCTCGAGG AGAATACGAT CCTGAAGGCC
    ACCGAAGTGC AGTTCTCCAA CAAGCCAACC GTTTCTAACG AGGCCAAGAG TTTCATTCGG
    GGATGCTTGG CCTATCGCAA GGAGGATCGC ATGGATGTGT TCGCACTGGC CAGGCACGAG
    TACATTCAGC CACCGATACC GAAACATGGG CGCGGTTCGC TCAATCAGCA ACAGCAGGCG
    CAACAACAGC AGCAGCAACA ACAGCAACAG CAGCAGCAAC AGTCGTCGAC GTCACAGGCC
    AATTCTACAG GCCAGACATC TTTCTCTGCC CACATGTTTG GCAATATGAA TCAGTCGAGT
    TCGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02500
    Clone ID Related Accession (Same CDS sequence) NM_001297914.1 , NM_001144683.1 , NM_001272293.1
    Accession Version NM_001144683.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Tousled-like kinase, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCCCCG GCGCCCATTT GCAGATGTCC CCGCAGAACA CTTCGTCCCT AAGTCAACAC 
    CATCCACATC AACAGCAACA GTTACAACCC CCACAGCAGC AACAACAGCA TTTCCCTAAC
    CATCACAGCG CCCAGCAACA GTCGCAGCAG CAGCAGCAAC AGGAGCAACA GAATCCCCAG
    CAGCAGGCGC AACAGCAGCA GCAGATACTC CCACATCAAC ATTTGCAGCA CCTGCACAAG
    CATCCGCATC AGCTGCAACT GCATCAGCAG CAGCAACAAC AACTCCACCA GCAACAGCAG
    CAACACTTCC ACCAGCAGTC GCTGCAAGGG CTGCATCAGG GTAGCAGCAA TCCGGATTCG
    AATATGAGCA CTGGCTCCTC GCATAGCGAG AAGGATGTCA ATGATATGCT GAGTGGCGGT
    GCAGCAACGC CAGGAGCTGC AGCAGCAGCG ATTCAACAGC AACATCCCGC CTTTGCGCCC
    ACACTGGGAA TGCAGCAACC ACCGCCGCCC CCACCTCAAC ACTCCAATAA TGGAGGCGAG
    ATGGGCTACT TGTCGGCAGG CACGACCACG ACGACGTCGG TGTTAACGGT AGGCAAGCCT
    CGGACGCCAG CGGAGCGGAA ACGGAAGCGA AAAATGCCTC CATGTGCCAC TAGTGCGGAT
    GAGGCGGGGA GTGGCGGTGG CTCTGGCGGA GCAGGAGCAA CCGTTGTTAA CAACAGCAGC
    CTGAAGGGCA AATCATTGGC CTTTCGTGAT ATGCCCAAGG TAAACATGAG CCTGAATCTG
    GGCGATCGTC TGGGAGGATC TGCAGGAAGC GGAGTAGGAG CCGGTGGCGC CGGAAGCGGG
    GGAGGTGGCG CTGGTTCCGG TTCTGGAAGC GGTGGCGGCA AAAGCGCCCG CCTGATGCTG
    CCAGTCAGCG ACAACAAGAA GATCAACGAC TATTTCAATA AGCAGCAAAC GGGCGTGGGC
    GTCGGTGTGC CAGGTGGTGC GGGAGGCAAT ACCGCTGGCC TTCGAGGATC ACATACGGGA
    GGTGGCAGCA AGTCACCCTC ATCCGCCCAG CAGCAGCAAA CGGCGGCACA GCAGCAGGGA
    AGCGGTGTTG CGACGGGAGG CAGTGCAGGC GGTTCCGCTG GCAACCAGGT GCAAGTGCAA
    ACGAGCAGCG CTTACGCCCT TTACCCACCA GCTAGTCCCC AAACCCAGAC GTCACAGCAA
    CAGCAGCAGC AGCAACCGGG ATCAGACTTT CACTATGTCA ACTCCAGCAA GGCGCAGCAA
    CAACAGCAGC GTCAACAGCA ACAGACTTCC AATCAAATGG TTCCTCCACA CGTGGTCGTT
    GGCCTTGGTG GTCATCCACT GAGCCTCGCG TCCATTCAGC AGCAGACGCC CTTATCCCAG
    CAGCAACAGC AGCAACAACA GCAGCAGCAA CAGCAGCAAC TGGGACCACC GACCACATCG
    ACGGCCTCCG TCGTGCCAAC GCATCCGCAT CAACTCGGAT CCCTGGGAGT TGTTGGGATG
    GTCGGTGTGG GTGTTGGCGT GGGCGTTGGA GTAAATGTGG GTGTGGGACC ACCACTGCCA
    CCACCACCGC CGATGGCCAT GCCAGCGGCC ATTATCACTT ATAGTAAGGC CACTCAAACG
    GAGGTGTCGC TGCATGAATT GCAGGAGCGC GAAGCGGAGC ACGAATCGGG CAAGGTGAAG
    CTAGACGAGA TGACACGGCT GTCCGATGAA CAAAAGTCCC AAATTGTTGG CAACCAGAAG
    ACGATTGACC AGCACAAGTG CCACATAGCC AAGTGTATTG ATGTGGTCAA GAAGCTGTTG
    AAGGAGAAGA GCAGCATCGA GAAGAAGGAG GCGCGACAGA AGTGCATGCA GAATCGCCTC
    AGGCTCGGAC AGTTTGTTAC CCAACGAGTG GGCGCCACAT TCCAGGAGAA CTGGACGGAC
    GGCTATGCGT TCCAGGAGCT GAGTCGGCGG CAAGAAGAAA TAACCGCTGA GCGTGAAGAG
    ATAGATCGGC AGAAAAAGCA GCTGATGAAA AAGCGTCCGG CGGAGTCCGG ACGCAAGCGC
    AACAACAACA GTAACCAGAA CAACCAGCAG CAGCAGCAAC AGCAACACCA GCAACAGCAG
    CAGCAACAAA ATTCCAACTC GAACGATTCC ACGCAGCTGA CGAGCGGAGT TGTTACCGGT
    CCAGGCAGTG ATCGTGTGAG CGTAAGCGTC GACAGCGGAT TGGGTGGCAA TAATGCGGGC
    GCGATCGGTG GCGGAACCGT TGGTGGTGGC GTTGGAGGTG GTGGTGTTGG AGGCGGTGGT
    GTCGGAGGCG GCGGTGGACG TGGACTTTCT CGCAGCAATT CGACGCAGGC CAATCAGGCT
    CAATTGCTGC ACAACGGCGG TGGTGGTTCG GGCGGCAATG TCGGCAACTC GGGCGGCGTT
    GGCGACCGCT TGTCAGATCG AGGAGGAGGA GGTGGCGGCA TCGGCGGAAA CGATAGCGGC
    AGCTGCTCGG ACTCGGGCAC TTTCCTGAAG CCAGACCCCG TATCGGGTGC CTACACAGCG
    CAGGAGTATT ACGAGTACGA TGAGATCCTC AAGTTGCGAC AAAATGCCCT CAAAAAGGAG
    GACGCCGACC TGCAGCTGGA GATGGAGAAG CTGGAGCGGG AGCGCAATCT GCACATCCGA
    GAGCTCAAGC GGATTCTTAA CGAGGATCAG TCCCGCTTTA ACAATCATCC CGTGCTGAAT
    GATCGCTATC TTCTGTTGAT GCTCCTGGGC AAGGGCGGCT TCTCAGAGGT CCACAAGGCC
    TTCGACCTGA AGGAGCAACG CTATGTCGCA TGTAAGGTGC ACCAATTAAA CAAGGATTGG
    AAGGAGGATA AGAAAGCTAA TTATATCAAA CACGCTTTGC GGGAATACAA CATTCACAAG
    GCACTGGATC ATCCGCGGGT CGTCAAGCTA TACGATGTCT TCGAGATCGA TGCGAATTCC
    TTTTGCACAG TGCTCGAATA CTGTGATGGC CACGATCTGG ACTTCTATTT GAAGCAACAT
    AAGACTATAC CCGAGCGTGA AGCGCGCTCG ATAATAATGC AGGTTGTATC TGCACTCAAG
    TATCTAAATG AGATTAAGCC TCCAGTTATC CACTACGATC TGAAGCCCGG CAACATTCTG
    CTTACCGAGG GCAACGTCTG CGGCGAGATT AAGATCACCG ACTTCGGTCT GTCAAAGGTG
    ATGGACGACG AGAATTACAA TCCCGATCAC GGCATGGATC TGACCTCTCA GGGGGCGGGA
    ACCTACTGGT ATCTGCCACC CGAGTGCTTT GTCGTGGGCA AAAATCCGCC GAAAATCTCC
    TCCAAAGTGG ACGTATGGAG TGTGGGTGTT ATCTTCTACC AGTGTCTGTA CGGCAAAAAG
    CCCTTCGGTC ACAATCAGTC GCAGGCCACG ATTCTCGAGG AGAATACGAT CCTGAAGGCC
    ACCGAAGTGC AGTTCTCCAA CAAGCCAACC GTTTCTAACG AGGCCAAGAG TTTCATTCGG
    GGATGCTTGG CCTATCGCAA GGAGGATCGC ATGGATGTGT TCGCACTGGC CAGGCACGAG
    TACATTCAGC CACCGATACC GAAACATGGG CGCGGTTCGC TCAATCAGCA ACAGCAGGCG
    CAACAACAGC AGCAGCAACA ACAGCAACAG CAGCAGCAAC AGTCGTCGAC GTCACAGGCC
    AATTCTACAG GCCAGACATC TTTCTCTGCC CACATGTTTG GCAATATGAA TCAGTCGAGT
    TCGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138155.1
    CDS380..4108
    Translation

    Target ORF information:

    RefSeq Version NM_001144683.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144683.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCCCCG GCGCCCATTT GCAGATGTCC CCGCAGAACA CTTCGTCCCT AAGTCAACAC 
    CATCCACATC AACAGCAACA GTTACAACCC CCACAGCAGC AACAACAGCA TTTCCCTAAC
    CATCACAGCG CCCAGCAACA GTCGCAGCAG CAGCAGCAAC AGGAGCAACA GAATCCCCAG
    CAGCAGGCGC AACAGCAGCA GCAGATACTC CCACATCAAC ATTTGCAGCA CCTGCACAAG
    CATCCGCATC AGCTGCAACT GCATCAGCAG CAGCAACAAC AACTCCACCA GCAACAGCAG
    CAACACTTCC ACCAGCAGTC GCTGCAAGGG CTGCATCAGG GTAGCAGCAA TCCGGATTCG
    AATATGAGCA CTGGCTCCTC GCATAGCGAG AAGGATGTCA ATGATATGCT GAGTGGCGGT
    GCAGCAACGC CAGGAGCTGC AGCAGCAGCG ATTCAACAGC AACATCCCGC CTTTGCGCCC
    ACACTGGGAA TGCAGCAACC ACCGCCGCCC CCACCTCAAC ACTCCAATAA TGGAGGCGAG
    ATGGGCTACT TGTCGGCAGG CACGACCACG ACGACGTCGG TGTTAACGGT AGGCAAGCCT
    CGGACGCCAG CGGAGCGGAA ACGGAAGCGA AAAATGCCTC CATGTGCCAC TAGTGCGGAT
    GAGGCGGGGA GTGGCGGTGG CTCTGGCGGA GCAGGAGCAA CCGTTGTTAA CAACAGCAGC
    CTGAAGGGCA AATCATTGGC CTTTCGTGAT ATGCCCAAGG TAAACATGAG CCTGAATCTG
    GGCGATCGTC TGGGAGGATC TGCAGGAAGC GGAGTAGGAG CCGGTGGCGC CGGAAGCGGG
    GGAGGTGGCG CTGGTTCCGG TTCTGGAAGC GGTGGCGGCA AAAGCGCCCG CCTGATGCTG
    CCAGTCAGCG ACAACAAGAA GATCAACGAC TATTTCAATA AGCAGCAAAC GGGCGTGGGC
    GTCGGTGTGC CAGGTGGTGC GGGAGGCAAT ACCGCTGGCC TTCGAGGATC ACATACGGGA
    GGTGGCAGCA AGTCACCCTC ATCCGCCCAG CAGCAGCAAA CGGCGGCACA GCAGCAGGGA
    AGCGGTGTTG CGACGGGAGG CAGTGCAGGC GGTTCCGCTG GCAACCAGGT GCAAGTGCAA
    ACGAGCAGCG CTTACGCCCT TTACCCACCA GCTAGTCCCC AAACCCAGAC GTCACAGCAA
    CAGCAGCAGC AGCAACCGGG ATCAGACTTT CACTATGTCA ACTCCAGCAA GGCGCAGCAA
    CAACAGCAGC GTCAACAGCA ACAGACTTCC AATCAAATGG TTCCTCCACA CGTGGTCGTT
    GGCCTTGGTG GTCATCCACT GAGCCTCGCG TCCATTCAGC AGCAGACGCC CTTATCCCAG
    CAGCAACAGC AGCAACAACA GCAGCAGCAA CAGCAGCAAC TGGGACCACC GACCACATCG
    ACGGCCTCCG TCGTGCCAAC GCATCCGCAT CAACTCGGAT CCCTGGGAGT TGTTGGGATG
    GTCGGTGTGG GTGTTGGCGT GGGCGTTGGA GTAAATGTGG GTGTGGGACC ACCACTGCCA
    CCACCACCGC CGATGGCCAT GCCAGCGGCC ATTATCACTT ATAGTAAGGC CACTCAAACG
    GAGGTGTCGC TGCATGAATT GCAGGAGCGC GAAGCGGAGC ACGAATCGGG CAAGGTGAAG
    CTAGACGAGA TGACACGGCT GTCCGATGAA CAAAAGTCCC AAATTGTTGG CAACCAGAAG
    ACGATTGACC AGCACAAGTG CCACATAGCC AAGTGTATTG ATGTGGTCAA GAAGCTGTTG
    AAGGAGAAGA GCAGCATCGA GAAGAAGGAG GCGCGACAGA AGTGCATGCA GAATCGCCTC
    AGGCTCGGAC AGTTTGTTAC CCAACGAGTG GGCGCCACAT TCCAGGAGAA CTGGACGGAC
    GGCTATGCGT TCCAGGAGCT GAGTCGGCGG CAAGAAGAAA TAACCGCTGA GCGTGAAGAG
    ATAGATCGGC AGAAAAAGCA GCTGATGAAA AAGCGTCCGG CGGAGTCCGG ACGCAAGCGC
    AACAACAACA GTAACCAGAA CAACCAGCAG CAGCAGCAAC AGCAACACCA GCAACAGCAG
    CAGCAACAAA ATTCCAACTC GAACGATTCC ACGCAGCTGA CGAGCGGAGT TGTTACCGGT
    CCAGGCAGTG ATCGTGTGAG CGTAAGCGTC GACAGCGGAT TGGGTGGCAA TAATGCGGGC
    GCGATCGGTG GCGGAACCGT TGGTGGTGGC GTTGGAGGTG GTGGTGTTGG AGGCGGTGGT
    GTCGGAGGCG GCGGTGGACG TGGACTTTCT CGCAGCAATT CGACGCAGGC CAATCAGGCT
    CAATTGCTGC ACAACGGCGG TGGTGGTTCG GGCGGCAATG TCGGCAACTC GGGCGGCGTT
    GGCGACCGCT TGTCAGATCG AGGAGGAGGA GGTGGCGGCA TCGGCGGAAA CGATAGCGGC
    AGCTGCTCGG ACTCGGGCAC TTTCCTGAAG CCAGACCCCG TATCGGGTGC CTACACAGCG
    CAGGAGTATT ACGAGTACGA TGAGATCCTC AAGTTGCGAC AAAATGCCCT CAAAAAGGAG
    GACGCCGACC TGCAGCTGGA GATGGAGAAG CTGGAGCGGG AGCGCAATCT GCACATCCGA
    GAGCTCAAGC GGATTCTTAA CGAGGATCAG TCCCGCTTTA ACAATCATCC CGTGCTGAAT
    GATCGCTATC TTCTGTTGAT GCTCCTGGGC AAGGGCGGCT TCTCAGAGGT CCACAAGGCC
    TTCGACCTGA AGGAGCAACG CTATGTCGCA TGTAAGGTGC ACCAATTAAA CAAGGATTGG
    AAGGAGGATA AGAAAGCTAA TTATATCAAA CACGCTTTGC GGGAATACAA CATTCACAAG
    GCACTGGATC ATCCGCGGGT CGTCAAGCTA TACGATGTCT TCGAGATCGA TGCGAATTCC
    TTTTGCACAG TGCTCGAATA CTGTGATGGC CACGATCTGG ACTTCTATTT GAAGCAACAT
    AAGACTATAC CCGAGCGTGA AGCGCGCTCG ATAATAATGC AGGTTGTATC TGCACTCAAG
    TATCTAAATG AGATTAAGCC TCCAGTTATC CACTACGATC TGAAGCCCGG CAACATTCTG
    CTTACCGAGG GCAACGTCTG CGGCGAGATT AAGATCACCG ACTTCGGTCT GTCAAAGGTG
    ATGGACGACG AGAATTACAA TCCCGATCAC GGCATGGATC TGACCTCTCA GGGGGCGGGA
    ACCTACTGGT ATCTGCCACC CGAGTGCTTT GTCGTGGGCA AAAATCCGCC GAAAATCTCC
    TCCAAAGTGG ACGTATGGAG TGTGGGTGTT ATCTTCTACC AGTGTCTGTA CGGCAAAAAG
    CCCTTCGGTC ACAATCAGTC GCAGGCCACG ATTCTCGAGG AGAATACGAT CCTGAAGGCC
    ACCGAAGTGC AGTTCTCCAA CAAGCCAACC GTTTCTAACG AGGCCAAGAG TTTCATTCGG
    GGATGCTTGG CCTATCGCAA GGAGGATCGC ATGGATGTGT TCGCACTGGC CAGGCACGAG
    TACATTCAGC CACCGATACC GAAACATGGG CGCGGTTCGC TCAATCAGCA ACAGCAGGCG
    CAACAACAGC AGCAGCAACA ACAGCAACAG CAGCAGCAAC AGTCGTCGAC GTCACAGGCC
    AATTCTACAG GCCAGACATC TTTCTCTGCC CACATGTTTG GCAATATGAA TCAGTCGAGT
    TCGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02500
    Clone ID Related Accession (Same CDS sequence) NM_001297914.1 , NM_001144683.1 , NM_001272293.1
    Accession Version NM_001272293.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Tousled-like kinase, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCCCCG GCGCCCATTT GCAGATGTCC CCGCAGAACA CTTCGTCCCT AAGTCAACAC 
    CATCCACATC AACAGCAACA GTTACAACCC CCACAGCAGC AACAACAGCA TTTCCCTAAC
    CATCACAGCG CCCAGCAACA GTCGCAGCAG CAGCAGCAAC AGGAGCAACA GAATCCCCAG
    CAGCAGGCGC AACAGCAGCA GCAGATACTC CCACATCAAC ATTTGCAGCA CCTGCACAAG
    CATCCGCATC AGCTGCAACT GCATCAGCAG CAGCAACAAC AACTCCACCA GCAACAGCAG
    CAACACTTCC ACCAGCAGTC GCTGCAAGGG CTGCATCAGG GTAGCAGCAA TCCGGATTCG
    AATATGAGCA CTGGCTCCTC GCATAGCGAG AAGGATGTCA ATGATATGCT GAGTGGCGGT
    GCAGCAACGC CAGGAGCTGC AGCAGCAGCG ATTCAACAGC AACATCCCGC CTTTGCGCCC
    ACACTGGGAA TGCAGCAACC ACCGCCGCCC CCACCTCAAC ACTCCAATAA TGGAGGCGAG
    ATGGGCTACT TGTCGGCAGG CACGACCACG ACGACGTCGG TGTTAACGGT AGGCAAGCCT
    CGGACGCCAG CGGAGCGGAA ACGGAAGCGA AAAATGCCTC CATGTGCCAC TAGTGCGGAT
    GAGGCGGGGA GTGGCGGTGG CTCTGGCGGA GCAGGAGCAA CCGTTGTTAA CAACAGCAGC
    CTGAAGGGCA AATCATTGGC CTTTCGTGAT ATGCCCAAGG TAAACATGAG CCTGAATCTG
    GGCGATCGTC TGGGAGGATC TGCAGGAAGC GGAGTAGGAG CCGGTGGCGC CGGAAGCGGG
    GGAGGTGGCG CTGGTTCCGG TTCTGGAAGC GGTGGCGGCA AAAGCGCCCG CCTGATGCTG
    CCAGTCAGCG ACAACAAGAA GATCAACGAC TATTTCAATA AGCAGCAAAC GGGCGTGGGC
    GTCGGTGTGC CAGGTGGTGC GGGAGGCAAT ACCGCTGGCC TTCGAGGATC ACATACGGGA
    GGTGGCAGCA AGTCACCCTC ATCCGCCCAG CAGCAGCAAA CGGCGGCACA GCAGCAGGGA
    AGCGGTGTTG CGACGGGAGG CAGTGCAGGC GGTTCCGCTG GCAACCAGGT GCAAGTGCAA
    ACGAGCAGCG CTTACGCCCT TTACCCACCA GCTAGTCCCC AAACCCAGAC GTCACAGCAA
    CAGCAGCAGC AGCAACCGGG ATCAGACTTT CACTATGTCA ACTCCAGCAA GGCGCAGCAA
    CAACAGCAGC GTCAACAGCA ACAGACTTCC AATCAAATGG TTCCTCCACA CGTGGTCGTT
    GGCCTTGGTG GTCATCCACT GAGCCTCGCG TCCATTCAGC AGCAGACGCC CTTATCCCAG
    CAGCAACAGC AGCAACAACA GCAGCAGCAA CAGCAGCAAC TGGGACCACC GACCACATCG
    ACGGCCTCCG TCGTGCCAAC GCATCCGCAT CAACTCGGAT CCCTGGGAGT TGTTGGGATG
    GTCGGTGTGG GTGTTGGCGT GGGCGTTGGA GTAAATGTGG GTGTGGGACC ACCACTGCCA
    CCACCACCGC CGATGGCCAT GCCAGCGGCC ATTATCACTT ATAGTAAGGC CACTCAAACG
    GAGGTGTCGC TGCATGAATT GCAGGAGCGC GAAGCGGAGC ACGAATCGGG CAAGGTGAAG
    CTAGACGAGA TGACACGGCT GTCCGATGAA CAAAAGTCCC AAATTGTTGG CAACCAGAAG
    ACGATTGACC AGCACAAGTG CCACATAGCC AAGTGTATTG ATGTGGTCAA GAAGCTGTTG
    AAGGAGAAGA GCAGCATCGA GAAGAAGGAG GCGCGACAGA AGTGCATGCA GAATCGCCTC
    AGGCTCGGAC AGTTTGTTAC CCAACGAGTG GGCGCCACAT TCCAGGAGAA CTGGACGGAC
    GGCTATGCGT TCCAGGAGCT GAGTCGGCGG CAAGAAGAAA TAACCGCTGA GCGTGAAGAG
    ATAGATCGGC AGAAAAAGCA GCTGATGAAA AAGCGTCCGG CGGAGTCCGG ACGCAAGCGC
    AACAACAACA GTAACCAGAA CAACCAGCAG CAGCAGCAAC AGCAACACCA GCAACAGCAG
    CAGCAACAAA ATTCCAACTC GAACGATTCC ACGCAGCTGA CGAGCGGAGT TGTTACCGGT
    CCAGGCAGTG ATCGTGTGAG CGTAAGCGTC GACAGCGGAT TGGGTGGCAA TAATGCGGGC
    GCGATCGGTG GCGGAACCGT TGGTGGTGGC GTTGGAGGTG GTGGTGTTGG AGGCGGTGGT
    GTCGGAGGCG GCGGTGGACG TGGACTTTCT CGCAGCAATT CGACGCAGGC CAATCAGGCT
    CAATTGCTGC ACAACGGCGG TGGTGGTTCG GGCGGCAATG TCGGCAACTC GGGCGGCGTT
    GGCGACCGCT TGTCAGATCG AGGAGGAGGA GGTGGCGGCA TCGGCGGAAA CGATAGCGGC
    AGCTGCTCGG ACTCGGGCAC TTTCCTGAAG CCAGACCCCG TATCGGGTGC CTACACAGCG
    CAGGAGTATT ACGAGTACGA TGAGATCCTC AAGTTGCGAC AAAATGCCCT CAAAAAGGAG
    GACGCCGACC TGCAGCTGGA GATGGAGAAG CTGGAGCGGG AGCGCAATCT GCACATCCGA
    GAGCTCAAGC GGATTCTTAA CGAGGATCAG TCCCGCTTTA ACAATCATCC CGTGCTGAAT
    GATCGCTATC TTCTGTTGAT GCTCCTGGGC AAGGGCGGCT TCTCAGAGGT CCACAAGGCC
    TTCGACCTGA AGGAGCAACG CTATGTCGCA TGTAAGGTGC ACCAATTAAA CAAGGATTGG
    AAGGAGGATA AGAAAGCTAA TTATATCAAA CACGCTTTGC GGGAATACAA CATTCACAAG
    GCACTGGATC ATCCGCGGGT CGTCAAGCTA TACGATGTCT TCGAGATCGA TGCGAATTCC
    TTTTGCACAG TGCTCGAATA CTGTGATGGC CACGATCTGG ACTTCTATTT GAAGCAACAT
    AAGACTATAC CCGAGCGTGA AGCGCGCTCG ATAATAATGC AGGTTGTATC TGCACTCAAG
    TATCTAAATG AGATTAAGCC TCCAGTTATC CACTACGATC TGAAGCCCGG CAACATTCTG
    CTTACCGAGG GCAACGTCTG CGGCGAGATT AAGATCACCG ACTTCGGTCT GTCAAAGGTG
    ATGGACGACG AGAATTACAA TCCCGATCAC GGCATGGATC TGACCTCTCA GGGGGCGGGA
    ACCTACTGGT ATCTGCCACC CGAGTGCTTT GTCGTGGGCA AAAATCCGCC GAAAATCTCC
    TCCAAAGTGG ACGTATGGAG TGTGGGTGTT ATCTTCTACC AGTGTCTGTA CGGCAAAAAG
    CCCTTCGGTC ACAATCAGTC GCAGGCCACG ATTCTCGAGG AGAATACGAT CCTGAAGGCC
    ACCGAAGTGC AGTTCTCCAA CAAGCCAACC GTTTCTAACG AGGCCAAGAG TTTCATTCGG
    GGATGCTTGG CCTATCGCAA GGAGGATCGC ATGGATGTGT TCGCACTGGC CAGGCACGAG
    TACATTCAGC CACCGATACC GAAACATGGG CGCGGTTCGC TCAATCAGCA ACAGCAGGCG
    CAACAACAGC AGCAGCAACA ACAGCAACAG CAGCAGCAAC AGTCGTCGAC GTCACAGGCC
    AATTCTACAG GCCAGACATC TTTCTCTGCC CACATGTTTG GCAATATGAA TCAGTCGAGT
    TCGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259222.1
    CDS396..4124
    Translation

    Target ORF information:

    RefSeq Version NM_001272293.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCCCCG GCGCCCATTT GCAGATGTCC CCGCAGAACA CTTCGTCCCT AAGTCAACAC 
    CATCCACATC AACAGCAACA GTTACAACCC CCACAGCAGC AACAACAGCA TTTCCCTAAC
    CATCACAGCG CCCAGCAACA GTCGCAGCAG CAGCAGCAAC AGGAGCAACA GAATCCCCAG
    CAGCAGGCGC AACAGCAGCA GCAGATACTC CCACATCAAC ATTTGCAGCA CCTGCACAAG
    CATCCGCATC AGCTGCAACT GCATCAGCAG CAGCAACAAC AACTCCACCA GCAACAGCAG
    CAACACTTCC ACCAGCAGTC GCTGCAAGGG CTGCATCAGG GTAGCAGCAA TCCGGATTCG
    AATATGAGCA CTGGCTCCTC GCATAGCGAG AAGGATGTCA ATGATATGCT GAGTGGCGGT
    GCAGCAACGC CAGGAGCTGC AGCAGCAGCG ATTCAACAGC AACATCCCGC CTTTGCGCCC
    ACACTGGGAA TGCAGCAACC ACCGCCGCCC CCACCTCAAC ACTCCAATAA TGGAGGCGAG
    ATGGGCTACT TGTCGGCAGG CACGACCACG ACGACGTCGG TGTTAACGGT AGGCAAGCCT
    CGGACGCCAG CGGAGCGGAA ACGGAAGCGA AAAATGCCTC CATGTGCCAC TAGTGCGGAT
    GAGGCGGGGA GTGGCGGTGG CTCTGGCGGA GCAGGAGCAA CCGTTGTTAA CAACAGCAGC
    CTGAAGGGCA AATCATTGGC CTTTCGTGAT ATGCCCAAGG TAAACATGAG CCTGAATCTG
    GGCGATCGTC TGGGAGGATC TGCAGGAAGC GGAGTAGGAG CCGGTGGCGC CGGAAGCGGG
    GGAGGTGGCG CTGGTTCCGG TTCTGGAAGC GGTGGCGGCA AAAGCGCCCG CCTGATGCTG
    CCAGTCAGCG ACAACAAGAA GATCAACGAC TATTTCAATA AGCAGCAAAC GGGCGTGGGC
    GTCGGTGTGC CAGGTGGTGC GGGAGGCAAT ACCGCTGGCC TTCGAGGATC ACATACGGGA
    GGTGGCAGCA AGTCACCCTC ATCCGCCCAG CAGCAGCAAA CGGCGGCACA GCAGCAGGGA
    AGCGGTGTTG CGACGGGAGG CAGTGCAGGC GGTTCCGCTG GCAACCAGGT GCAAGTGCAA
    ACGAGCAGCG CTTACGCCCT TTACCCACCA GCTAGTCCCC AAACCCAGAC GTCACAGCAA
    CAGCAGCAGC AGCAACCGGG ATCAGACTTT CACTATGTCA ACTCCAGCAA GGCGCAGCAA
    CAACAGCAGC GTCAACAGCA ACAGACTTCC AATCAAATGG TTCCTCCACA CGTGGTCGTT
    GGCCTTGGTG GTCATCCACT GAGCCTCGCG TCCATTCAGC AGCAGACGCC CTTATCCCAG
    CAGCAACAGC AGCAACAACA GCAGCAGCAA CAGCAGCAAC TGGGACCACC GACCACATCG
    ACGGCCTCCG TCGTGCCAAC GCATCCGCAT CAACTCGGAT CCCTGGGAGT TGTTGGGATG
    GTCGGTGTGG GTGTTGGCGT GGGCGTTGGA GTAAATGTGG GTGTGGGACC ACCACTGCCA
    CCACCACCGC CGATGGCCAT GCCAGCGGCC ATTATCACTT ATAGTAAGGC CACTCAAACG
    GAGGTGTCGC TGCATGAATT GCAGGAGCGC GAAGCGGAGC ACGAATCGGG CAAGGTGAAG
    CTAGACGAGA TGACACGGCT GTCCGATGAA CAAAAGTCCC AAATTGTTGG CAACCAGAAG
    ACGATTGACC AGCACAAGTG CCACATAGCC AAGTGTATTG ATGTGGTCAA GAAGCTGTTG
    AAGGAGAAGA GCAGCATCGA GAAGAAGGAG GCGCGACAGA AGTGCATGCA GAATCGCCTC
    AGGCTCGGAC AGTTTGTTAC CCAACGAGTG GGCGCCACAT TCCAGGAGAA CTGGACGGAC
    GGCTATGCGT TCCAGGAGCT GAGTCGGCGG CAAGAAGAAA TAACCGCTGA GCGTGAAGAG
    ATAGATCGGC AGAAAAAGCA GCTGATGAAA AAGCGTCCGG CGGAGTCCGG ACGCAAGCGC
    AACAACAACA GTAACCAGAA CAACCAGCAG CAGCAGCAAC AGCAACACCA GCAACAGCAG
    CAGCAACAAA ATTCCAACTC GAACGATTCC ACGCAGCTGA CGAGCGGAGT TGTTACCGGT
    CCAGGCAGTG ATCGTGTGAG CGTAAGCGTC GACAGCGGAT TGGGTGGCAA TAATGCGGGC
    GCGATCGGTG GCGGAACCGT TGGTGGTGGC GTTGGAGGTG GTGGTGTTGG AGGCGGTGGT
    GTCGGAGGCG GCGGTGGACG TGGACTTTCT CGCAGCAATT CGACGCAGGC CAATCAGGCT
    CAATTGCTGC ACAACGGCGG TGGTGGTTCG GGCGGCAATG TCGGCAACTC GGGCGGCGTT
    GGCGACCGCT TGTCAGATCG AGGAGGAGGA GGTGGCGGCA TCGGCGGAAA CGATAGCGGC
    AGCTGCTCGG ACTCGGGCAC TTTCCTGAAG CCAGACCCCG TATCGGGTGC CTACACAGCG
    CAGGAGTATT ACGAGTACGA TGAGATCCTC AAGTTGCGAC AAAATGCCCT CAAAAAGGAG
    GACGCCGACC TGCAGCTGGA GATGGAGAAG CTGGAGCGGG AGCGCAATCT GCACATCCGA
    GAGCTCAAGC GGATTCTTAA CGAGGATCAG TCCCGCTTTA ACAATCATCC CGTGCTGAAT
    GATCGCTATC TTCTGTTGAT GCTCCTGGGC AAGGGCGGCT TCTCAGAGGT CCACAAGGCC
    TTCGACCTGA AGGAGCAACG CTATGTCGCA TGTAAGGTGC ACCAATTAAA CAAGGATTGG
    AAGGAGGATA AGAAAGCTAA TTATATCAAA CACGCTTTGC GGGAATACAA CATTCACAAG
    GCACTGGATC ATCCGCGGGT CGTCAAGCTA TACGATGTCT TCGAGATCGA TGCGAATTCC
    TTTTGCACAG TGCTCGAATA CTGTGATGGC CACGATCTGG ACTTCTATTT GAAGCAACAT
    AAGACTATAC CCGAGCGTGA AGCGCGCTCG ATAATAATGC AGGTTGTATC TGCACTCAAG
    TATCTAAATG AGATTAAGCC TCCAGTTATC CACTACGATC TGAAGCCCGG CAACATTCTG
    CTTACCGAGG GCAACGTCTG CGGCGAGATT AAGATCACCG ACTTCGGTCT GTCAAAGGTG
    ATGGACGACG AGAATTACAA TCCCGATCAC GGCATGGATC TGACCTCTCA GGGGGCGGGA
    ACCTACTGGT ATCTGCCACC CGAGTGCTTT GTCGTGGGCA AAAATCCGCC GAAAATCTCC
    TCCAAAGTGG ACGTATGGAG TGTGGGTGTT ATCTTCTACC AGTGTCTGTA CGGCAAAAAG
    CCCTTCGGTC ACAATCAGTC GCAGGCCACG ATTCTCGAGG AGAATACGAT CCTGAAGGCC
    ACCGAAGTGC AGTTCTCCAA CAAGCCAACC GTTTCTAACG AGGCCAAGAG TTTCATTCGG
    GGATGCTTGG CCTATCGCAA GGAGGATCGC ATGGATGTGT TCGCACTGGC CAGGCACGAG
    TACATTCAGC CACCGATACC GAAACATGGG CGCGGTTCGC TCAATCAGCA ACAGCAGGCG
    CAACAACAGC AGCAGCAACA ACAGCAACAG CAGCAGCAAC AGTCGTCGAC GTCACAGGCC
    AATTCTACAG GCCAGACATC TTTCTCTGCC CACATGTTTG GCAATATGAA TCAGTCGAGT
    TCGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02927
    Clone ID Related Accession (Same CDS sequence) NM_206620.6
    Accession Version NM_206620.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3768bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Tousled-like kinase, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 26, 2009 this sequence version replaced NM_206620.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCTGTGG TAAAACAGCG GAAAATGAGC CGCTTTAAGA TGTCCCCCGG CGCCCATTTG 
    CAGATGTCCC CGCAGAACAC TTCGTCCCTA AGTCAACACC ATCCACATCA ACAGCAACAG
    TTACAACCCC CACAGCAGCA ACAACAGCAT TTCCCTAACC ATCACAGCGC CCAGCAACAG
    TCGCAGCAGC AGCAGCAACA GGAGCAACAG AATCCCCAGC AGCAGGCGCA ACAGCAGCAG
    CAGATACTCC CACATCAACA TTTGCAGCAC CTGCACAAGC ATCCGCATCA GCTGCAACTG
    CATCAGCAGC AGCAACAACA ACTCCACCAG CAACAGCAGC AACACTTCCA CCAGCAGTCG
    CTGCAAGGGC TGCATCAGGG TAGCAGCAAT CCGGATTCGA ATATGAGCAC TGGCTCCTCG
    CATAGCGAGA AGGATGTCAA TGATATGCTG AGTGGCGGTG CAGCAACGCC AGGAGCTGCA
    GCAGCAGCGA TTCAACAGCA ACATCCCGCC TTTGCGCCCA CACTGGGAAT GCAGCAACCA
    CCGCCGCCCC CACCTCAACA CTCCAATAAT GGAGGCGAGA TGGGCTACTT GTCGGCAGGC
    ACGACCACGA CGACGTCGGT GTTAACGGTA GGCAAGCCTC GGACGCCAGC GGAGCGGAAA
    CGGAAGCGAA AAATGCCTCC ATGTGCCACT AGTGCGGATG AGGCGGGGAG TGGCGGTGGC
    TCTGGCGGAG CAGGAGCAAC CGTTGTTAAC AACAGCAGCC TGAAGGGCAA ATCATTGGCC
    TTTCGTGATA TGCCCAAGGT AAACATGAGC CTGAATCTGG GCGATCGTCT GGGAGGATCT
    GCAGGAAGCG GAGTAGGAGC CGGTGGCGCC GGAAGCGGGG GAGGTGGCGC TGGTTCCGGT
    TCTGGAAGCG GTGGCGGCAA AAGCGCCCGC CTGATGCTGC CAGTCAGCGA CAACAAGAAG
    ATCAACGACT ATTTCAATAA GCAGCAAACG GGCGTGGGCG TCGGTGTGCC AGGTGGTGCG
    GGAGGCAATA CCGCTGGCCT TCGAGGATCA CATACGGGAG GTGGCAGCAA GTCACCCTCA
    TCCGCCCAGC AGCAGCAAAC GGCGGCACAG CAGCAGGGAA GCGGTGTTGC GACGGGAGGC
    AGTGCAGGCG GTTCCGCTGG CAACCAGGTG CAAGTGCAAA CGAGCAGCGC TTACGCCCTT
    TACCCACCAG CTAGTCCCCA AACCCAGACG TCACAGCAAC AGCAGCAGCA GCAACCGGGA
    TCAGACTTTC ACTATGTCAA CTCCAGCAAG GCGCAGCAAC AACAGCAGCG TCAACAGCAA
    CAGACTTCCA ATCAAATGGT TCCTCCACAC GTGGTCGTTG GCCTTGGTGG TCATCCACTG
    AGCCTCGCGT CCATTCAGCA GCAGACGCCC TTATCCCAGC AGCAACAGCA GCAACAACAG
    CAGCAGCAAC AGCAGCAACT GGGACCACCG ACCACATCGA CGGCCTCCGT CGTGCCAACG
    CATCCGCATC AACTCGGATC CCTGGGAGTT GTTGGGATGG TCGGTGTGGG TGTTGGCGTG
    GGCGTTGGAG TAAATGTGGG TGTGGGACCA CCACTGCCAC CACCACCGCC GATGGCCATG
    CCAGCGGCCA TTATCACTTA TAGTAAGGCC ACTCAAACGG AGGTGTCGCT GCATGAATTG
    CAGGAGCGCG AAGCGGAGCA CGAATCGGGC AAGGTGAAGC TAGACGAGAT GACACGGCTG
    TCCGATGAAC AAAAGTCCCA AATTGTTGGC AACCAGAAGA CGATTGACCA GCACAAGTGC
    CACATAGCCA AGTGTATTGA TGTGGTCAAG AAGCTGTTGA AGGAGAAGAG CAGCATCGAG
    AAGAAGGAGG CGCGACAGAA GTGCATGCAG AATCGCCTCA GGCTCGGACA GTTTGTTACC
    CAACGAGTGG GCGCCACATT CCAGGAGAAC TGGACGGACG GCTATGCGTT CCAGGAGCTG
    AGTCGGCGGC AAGAAGAAAT AACCGCTGAG CGTGAAGAGA TAGATCGGCA GAAAAAGCAG
    CTGATGAAAA AGCGTCCGGC GGAGTCCGGA CGCAAGCGCA ACAACAACAG TAACCAGAAC
    AACCAGCAGC AGCAGCAACA GCAACACCAG CAACAGCAGC AGCAACAAAA TTCCAACTCG
    AACGATTCCA CGCAGCTGAC GAGCGGAGTT GTTACCGGTC CAGGCAGTGA TCGTGTGAGC
    GTAAGCGTCG ACAGCGGATT GGGTGGCAAT AATGCGGGCG CGATCGGTGG CGGAACCGTT
    GGTGGTGGCG TTGGAGGTGG TGGTGTTGGA GGCGGTGGTG TCGGAGGCGG CGGTGGACGT
    GGACTTTCTC GCAGCAATTC GACGCAGGCC AATCAGGCTC AATTGCTGCA CAACGGCGGT
    GGTGGTTCGG GCGGCAATGT CGGCAACTCG GGCGGCGTTG GCGACCGCTT GTCAGATCGA
    GGAGGAGGAG GTGGCGGCAT CGGCGGAAAC GATAGCGGCA GCTGCTCGGA CTCGGGCACT
    TTCCTGAAGC CAGACCCCGT ATCGGGTGCC TACACAGCGC AGGAGTATTA CGAGTACGAT
    GAGATCCTCA AGTTGCGACA AAATGCCCTC AAAAAGGAGG ACGCCGACCT GCAGCTGGAG
    ATGGAGAAGC TGGAGCGGGA GCGCAATCTG CACATCCGAG AGCTCAAGCG GATTCTTAAC
    GAGGATCAGT CCCGCTTTAA CAATCATCCC GTGCTGAATG ATCGCTATCT TCTGTTGATG
    CTCCTGGGCA AGGGCGGCTT CTCAGAGGTC CACAAGGCCT TCGACCTGAA GGAGCAACGC
    TATGTCGCAT GTAAGGTGCA CCAATTAAAC AAGGATTGGA AGGAGGATAA GAAAGCTAAT
    TATATCAAAC ACGCTTTGCG GGAATACAAC ATTCACAAGG CACTGGATCA TCCGCGGGTC
    GTCAAGCTAT ACGATGTCTT CGAGATCGAT GCGAATTCCT TTTGCACAGT GCTCGAATAC
    TGTGATGGCC ACGATCTGGA CTTCTATTTG AAGCAACATA AGACTATACC CGAGCGTGAA
    GCGCGCTCGA TAATAATGCA GGTTGTATCT GCACTCAAGT ATCTAAATGA GATTAAGCCT
    CCAGTTATCC ACTACGATCT GAAGCCCGGC AACATTCTGC TTACCGAGGG CAACGTCTGC
    GGCGAGATTA AGATCACCGA CTTCGGTCTG TCAAAGGTGA TGGACGACGA GAATTACAAT
    CCCGATCACG GCATGGATCT GACCTCTCAG GGGGCGGGAA CCTACTGGTA TCTGCCACCC
    GAGTGCTTTG TCGTGGGCAA AAATCCGCCG AAAATCTCCT CCAAAGTGGA CGTATGGAGT
    GTGGGTGTTA TCTTCTACCA GTGTCTGTAC GGCAAAAAGC CCTTCGGTCA CAATCAGTCG
    CAGGCCACGA TTCTCGAGGA GAATACGATC CTGAAGGCCA CCGAAGTGCA GTTCTCCAAC
    AAGCCAACCG TTTCTAACGA GGCCAAGAGT TTCATTCGGG GATGCTTGGC CTATCGCAAG
    GAGGATCGCA TGGATGTGTT CGCACTGGCC AGGCACGAGT ACATTCAGCC ACCGATACCG
    AAACATGGGC GCGGTTCGCT CAATCAGCAA CAGCAGGCGC AACAACAGCA GCAGCAACAA
    CAGCAACAGC AGCAGCAACA GTCGTCGACG TCACAGGCCA ATTCTACAGG CCAGACATCT
    TTCTCTGCCC ACATGTTTGG CAATATGAAT CAGTCGAGTT CGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996343.5
    CDS197..3964
    Translation

    Target ORF information:

    RefSeq Version NM_206620.6
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206620.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCTGTGG TAAAACAGCG GAAAATGAGC CGCTTTAAGA TGTCCCCCGG CGCCCATTTG 
    CAGATGTCCC CGCAGAACAC TTCGTCCCTA AGTCAACACC ATCCACATCA ACAGCAACAG
    TTACAACCCC CACAGCAGCA ACAACAGCAT TTCCCTAACC ATCACAGCGC CCAGCAACAG
    TCGCAGCAGC AGCAGCAACA GGAGCAACAG AATCCCCAGC AGCAGGCGCA ACAGCAGCAG
    CAGATACTCC CACATCAACA TTTGCAGCAC CTGCACAAGC ATCCGCATCA GCTGCAACTG
    CATCAGCAGC AGCAACAACA ACTCCACCAG CAACAGCAGC AACACTTCCA CCAGCAGTCG
    CTGCAAGGGC TGCATCAGGG TAGCAGCAAT CCGGATTCGA ATATGAGCAC TGGCTCCTCG
    CATAGCGAGA AGGATGTCAA TGATATGCTG AGTGGCGGTG CAGCAACGCC AGGAGCTGCA
    GCAGCAGCGA TTCAACAGCA ACATCCCGCC TTTGCGCCCA CACTGGGAAT GCAGCAACCA
    CCGCCGCCCC CACCTCAACA CTCCAATAAT GGAGGCGAGA TGGGCTACTT GTCGGCAGGC
    ACGACCACGA CGACGTCGGT GTTAACGGTA GGCAAGCCTC GGACGCCAGC GGAGCGGAAA
    CGGAAGCGAA AAATGCCTCC ATGTGCCACT AGTGCGGATG AGGCGGGGAG TGGCGGTGGC
    TCTGGCGGAG CAGGAGCAAC CGTTGTTAAC AACAGCAGCC TGAAGGGCAA ATCATTGGCC
    TTTCGTGATA TGCCCAAGGT AAACATGAGC CTGAATCTGG GCGATCGTCT GGGAGGATCT
    GCAGGAAGCG GAGTAGGAGC CGGTGGCGCC GGAAGCGGGG GAGGTGGCGC TGGTTCCGGT
    TCTGGAAGCG GTGGCGGCAA AAGCGCCCGC CTGATGCTGC CAGTCAGCGA CAACAAGAAG
    ATCAACGACT ATTTCAATAA GCAGCAAACG GGCGTGGGCG TCGGTGTGCC AGGTGGTGCG
    GGAGGCAATA CCGCTGGCCT TCGAGGATCA CATACGGGAG GTGGCAGCAA GTCACCCTCA
    TCCGCCCAGC AGCAGCAAAC GGCGGCACAG CAGCAGGGAA GCGGTGTTGC GACGGGAGGC
    AGTGCAGGCG GTTCCGCTGG CAACCAGGTG CAAGTGCAAA CGAGCAGCGC TTACGCCCTT
    TACCCACCAG CTAGTCCCCA AACCCAGACG TCACAGCAAC AGCAGCAGCA GCAACCGGGA
    TCAGACTTTC ACTATGTCAA CTCCAGCAAG GCGCAGCAAC AACAGCAGCG TCAACAGCAA
    CAGACTTCCA ATCAAATGGT TCCTCCACAC GTGGTCGTTG GCCTTGGTGG TCATCCACTG
    AGCCTCGCGT CCATTCAGCA GCAGACGCCC TTATCCCAGC AGCAACAGCA GCAACAACAG
    CAGCAGCAAC AGCAGCAACT GGGACCACCG ACCACATCGA CGGCCTCCGT CGTGCCAACG
    CATCCGCATC AACTCGGATC CCTGGGAGTT GTTGGGATGG TCGGTGTGGG TGTTGGCGTG
    GGCGTTGGAG TAAATGTGGG TGTGGGACCA CCACTGCCAC CACCACCGCC GATGGCCATG
    CCAGCGGCCA TTATCACTTA TAGTAAGGCC ACTCAAACGG AGGTGTCGCT GCATGAATTG
    CAGGAGCGCG AAGCGGAGCA CGAATCGGGC AAGGTGAAGC TAGACGAGAT GACACGGCTG
    TCCGATGAAC AAAAGTCCCA AATTGTTGGC AACCAGAAGA CGATTGACCA GCACAAGTGC
    CACATAGCCA AGTGTATTGA TGTGGTCAAG AAGCTGTTGA AGGAGAAGAG CAGCATCGAG
    AAGAAGGAGG CGCGACAGAA GTGCATGCAG AATCGCCTCA GGCTCGGACA GTTTGTTACC
    CAACGAGTGG GCGCCACATT CCAGGAGAAC TGGACGGACG GCTATGCGTT CCAGGAGCTG
    AGTCGGCGGC AAGAAGAAAT AACCGCTGAG CGTGAAGAGA TAGATCGGCA GAAAAAGCAG
    CTGATGAAAA AGCGTCCGGC GGAGTCCGGA CGCAAGCGCA ACAACAACAG TAACCAGAAC
    AACCAGCAGC AGCAGCAACA GCAACACCAG CAACAGCAGC AGCAACAAAA TTCCAACTCG
    AACGATTCCA CGCAGCTGAC GAGCGGAGTT GTTACCGGTC CAGGCAGTGA TCGTGTGAGC
    GTAAGCGTCG ACAGCGGATT GGGTGGCAAT AATGCGGGCG CGATCGGTGG CGGAACCGTT
    GGTGGTGGCG TTGGAGGTGG TGGTGTTGGA GGCGGTGGTG TCGGAGGCGG CGGTGGACGT
    GGACTTTCTC GCAGCAATTC GACGCAGGCC AATCAGGCTC AATTGCTGCA CAACGGCGGT
    GGTGGTTCGG GCGGCAATGT CGGCAACTCG GGCGGCGTTG GCGACCGCTT GTCAGATCGA
    GGAGGAGGAG GTGGCGGCAT CGGCGGAAAC GATAGCGGCA GCTGCTCGGA CTCGGGCACT
    TTCCTGAAGC CAGACCCCGT ATCGGGTGCC TACACAGCGC AGGAGTATTA CGAGTACGAT
    GAGATCCTCA AGTTGCGACA AAATGCCCTC AAAAAGGAGG ACGCCGACCT GCAGCTGGAG
    ATGGAGAAGC TGGAGCGGGA GCGCAATCTG CACATCCGAG AGCTCAAGCG GATTCTTAAC
    GAGGATCAGT CCCGCTTTAA CAATCATCCC GTGCTGAATG ATCGCTATCT TCTGTTGATG
    CTCCTGGGCA AGGGCGGCTT CTCAGAGGTC CACAAGGCCT TCGACCTGAA GGAGCAACGC
    TATGTCGCAT GTAAGGTGCA CCAATTAAAC AAGGATTGGA AGGAGGATAA GAAAGCTAAT
    TATATCAAAC ACGCTTTGCG GGAATACAAC ATTCACAAGG CACTGGATCA TCCGCGGGTC
    GTCAAGCTAT ACGATGTCTT CGAGATCGAT GCGAATTCCT TTTGCACAGT GCTCGAATAC
    TGTGATGGCC ACGATCTGGA CTTCTATTTG AAGCAACATA AGACTATACC CGAGCGTGAA
    GCGCGCTCGA TAATAATGCA GGTTGTATCT GCACTCAAGT ATCTAAATGA GATTAAGCCT
    CCAGTTATCC ACTACGATCT GAAGCCCGGC AACATTCTGC TTACCGAGGG CAACGTCTGC
    GGCGAGATTA AGATCACCGA CTTCGGTCTG TCAAAGGTGA TGGACGACGA GAATTACAAT
    CCCGATCACG GCATGGATCT GACCTCTCAG GGGGCGGGAA CCTACTGGTA TCTGCCACCC
    GAGTGCTTTG TCGTGGGCAA AAATCCGCCG AAAATCTCCT CCAAAGTGGA CGTATGGAGT
    GTGGGTGTTA TCTTCTACCA GTGTCTGTAC GGCAAAAAGC CCTTCGGTCA CAATCAGTCG
    CAGGCCACGA TTCTCGAGGA GAATACGATC CTGAAGGCCA CCGAAGTGCA GTTCTCCAAC
    AAGCCAACCG TTTCTAACGA GGCCAAGAGT TTCATTCGGG GATGCTTGGC CTATCGCAAG
    GAGGATCGCA TGGATGTGTT CGCACTGGCC AGGCACGAGT ACATTCAGCC ACCGATACCG
    AAACATGGGC GCGGTTCGCT CAATCAGCAA CAGCAGGCGC AACAACAGCA GCAGCAACAA
    CAGCAACAGC AGCAGCAACA GTCGTCGACG TCACAGGCCA ATTCTACAGG CCAGACATCT
    TTCTCTGCCC ACATGTTTGG CAATATGAAT CAGTCGAGTT CGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02928
    Clone ID Related Accession (Same CDS sequence) NM_001103403.1
    Accession Version NM_001103403.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Tousled-like kinase, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCCGCGAT TGCGATGCGA CGACGTTGCT GCCGTGCTGC TGCCCCTGAT GATGCTGTTA 
    TTGCTGTTGT TGCTGTTGTT GCGTCGTCTC GTGGTTGTCG TTGTCGGCCA CTTCATCATC
    GACAAGCAGC AAGAGCAAGA GCAAGAGCAG CAGCACCAGC AGCAGCAGCA GCAGCAGAGG
    CACAGGCTGT TGGCTAAAAC AAAGCAGAGG ACCCGGACCT CGGCCGACAA AAAGGACGAC
    TTGTGTCCTC ATCAATTCAG TTTCGGTTTC AGTCGCAATT CAAACGCCAT CAAAATCAAA
    ACGGCGCCCA GCTATCAACA ACATTTTCAT CATCAAAATC TTCACCACCA CCAACAACAA
    CATCAACAAC AACAACAACA ACAACATATA ACGCAAGTGC AACAACAACA ACAACAACAA
    CAGCAACTTT TGGTATTGCC ACAGCAGCAG CAACAGCAAC ATTTGCGAGT GTGCAACAAT
    CAAATCAATA ACAACAATAA CAGCAGTAAT AGCTGCCAAA ACAGCTATCA ATATAGTTAT
    AACAATAATA ATAACAACAC CAACGCCAAC AACAACAGCA ATCAGCAGCT GCAACAACAA
    ATTGTGAATA ACAACAACAA TCTCGAGGAC GATGTGTATT ATTTATTTTA TAAAGATTTT
    TATATGTCCC CCGGCGCCCA TTTGCAGATG TCCCCGCAGA ACACTTCGTC CCTAAGTCAA
    CACCATCCAC ATCAACAGCA ACAGTTACAA CCCCCACAGC AGCAACAACA GCATTTCCCT
    AACCATCACA GCGCCCAGCA ACAGTCGCAG CAGCAGCAGC AACAGGAGCA ACAGAATCCC
    CAGCAGCAGG CGCAACAGCA GCAGCAGATA CTCCCACATC AACATTTGCA GCACCTGCAC
    AAGCATCCGC ATCAGCTGCA ACTGCATCAG CAGCAGCAAC AACAACTCCA CCAGCAACAG
    CAGCAACACT TCCACCAGCA GTCGCTGCAA GGGCTGCATC AGGGTAGCAG CAATCCGGAT
    TCGAATATGA GCACTGGCTC CTCGCATAGC GAGAAGGATG TCAATGATAT GCTGAGTGGC
    GGTGCAGCAA CGCCAGGAGC TGCAGCAGCA GCGATTCAAC AGCAACATCC CGCCTTTGCG
    CCCACACTGG GAATGCAGCA ACCACCGCCG CCCCCACCTC AACACTCCAA TAATGGAGGC
    GAGATGGGCT ACTTGTCGGC AGGCACGACC ACGACGACGT CGGTGTTAAC GGTAGGCAAG
    CCTCGGACGC CAGCGGAGCG GAAACGGAAG CGAAAAATGC CTCCATGTGC CACTAGTGCG
    GATGAGGCGG GGAGTGGCGG TGGCTCTGGC GGAGCAGGAG CAACCGTTGT TAACAACAGC
    AGCCTGAAGG GCAAATCATT GGCCTTTCGT GATATGCCCA AGGTAAACAT GAGCCTGAAT
    CTGGGCGATC GTCTGGGAGG ATCTGCAGGA AGCGGAGTAG GAGCCGGTGG CGCCGGAAGC
    GGGGGAGGTG GCGCTGGTTC CGGTTCTGGA AGCGGTGGCG GCAAAAGCGC CCGCCTGATG
    CTGCCAGTCA GCGACAACAA GAAGATCAAC GACTATTTCA ATAAGCAGCA AACGGGCGTG
    GGCGTCGGTG TGCCAGGTGG TGCGGGAGGC AATACCGCTG GCCTTCGAGG ATCACATACG
    GGAGGTGGCA GCAAGTCACC CTCATCCGCC CAGCAGCAGC AAACGGCGGC ACAGCAGCAG
    GGAAGCGGTG TTGCGACGGG AGGCAGTGCA GGCGGTTCCG CTGGCAACCA GGTGCAAGTG
    CAAACGAGCA GCGCTTACGC CCTTTACCCA CCAGCTAGTC CCCAAACCCA GACGTCACAG
    CAACAGCAGC AGCAGCAACC GGGATCAGAC TTTCACTATG TCAACTCCAG CAAGGCGCAG
    CAACAACAGC AGCGTCAACA GCAACAGACT TCCAATCAAA TGGTTCCTCC ACACGTGGTC
    GTTGGCCTTG GTGGTCATCC ACTGAGCCTC GCGTCCATTC AGCAGCAGAC GCCCTTATCC
    CAGCAGCAAC AGCAGCAACA ACAGCAGCAG CAACAGCAGC AACTGGGACC ACCGACCACA
    TCGACGGCCT CCGTCGTGCC AACGCATCCG CATCAACTCG GATCCCTGGG AGTTGTTGGG
    ATGGTCGGTG TGGGTGTTGG CGTGGGCGTT GGAGTAAATG TGGGTGTGGG ACCACCACTG
    CCACCACCAC CGCCGATGGC CATGCCAGCG GCCATTATCA CTTATAGTAA GGCCACTCAA
    ACGGAGGTGT CGCTGCATGA ATTGCAGGAG CGCGAAGCGG AGCACGAATC GGGCAAGGTG
    AAGCTAGACG AGATGACACG GCTGTCCGAT GAACAAAAGT CCCAAATTGT TGGCAACCAG
    AAGACGATTG ACCAGCACAA GTGCCACATA GCCAAGTGTA TTGATGTGGT CAAGAAGCTG
    TTGAAGGAGA AGAGCAGCAT CGAGAAGAAG GAGGCGCGAC AGAAGTGCAT GCAGAATCGC
    CTCAGGCTCG GACAGTTTGT TACCCAACGA GTGGGCGCCA CATTCCAGGA GAACTGGACG
    GACGGCTATG CGTTCCAGGA GCTGAGTCGG CGGCAAGAAG AAATAACCGC TGAGCGTGAA
    GAGATAGATC GGCAGAAAAA GCAGCTGATG AAAAAGCGTC CGGCGGAGTC CGGACGCAAG
    CGCAACAACA ACAGTAACCA GAACAACCAG CAGCAGCAGC AACAGCAACA CCAGCAACAG
    CAGCAGCAAC AAAATTCCAA CTCGAACGAT TCCACGCAGC TGACGAGCGG AGTTGTTACC
    GGTCCAGGCA GTGATCGTGT GAGCGTAAGC GTCGACAGCG GATTGGGTGG CAATAATGCG
    GGCGCGATCG GTGGCGGAAC CGTTGGTGGT GGCGTTGGAG GTGGTGGTGT TGGAGGCGGT
    GGTGTCGGAG GCGGCGGTGG ACGTGGACTT TCTCGCAGCA ATTCGACGCA GGCCAATCAG
    GCTCAATTGC TGCACAACGG CGGTGGTGGT TCGGGCGGCA ATGTCGGCAA CTCGGGCGGC
    GTTGGCGACC GCTTGTCAGA TCGAGGAGGA GGAGGTGGCG GCATCGGCGG AAACGATAGC
    GGCAGCTGCT CGGACTCGGG CACTTTCCTG AAGCCAGACC CCGTATCGGG TGCCTACACA
    GCGCAGGAGT ATTACGAGTA CGATGAGATC CTCAAGTTGC GACAAAATGC CCTCAAAAAG
    GAGGACGCCG ACCTGCAGCT GGAGATGGAG AAGCTGGAGC GGGAGCGCAA TCTGCACATC
    CGAGAGCTCA AGCGGATTCT TAACGAGGAT CAGTCCCGCT TTAACAATCA TCCCGTGCTG
    AATGATCGCT ATCTTCTGTT GATGCTCCTG GGCAAGGGCG GCTTCTCAGA GGTCCACAAG
    GCCTTCGACC TGAAGGAGCA ACGCTATGTC GCATGTAAGG TGCACCAATT AAACAAGGAT
    TGGAAGGAGG ATAAGAAAGC TAATTATATC AAACACGCTT TGCGGGAATA CAACATTCAC
    AAGGCACTGG ATCATCCGCG GGTCGTCAAG CTATACGATG TCTTCGAGAT CGATGCGAAT
    TCCTTTTGCA CAGTGCTCGA ATACTGTGAT GGCCACGATC TGGACTTCTA TTTGAAGCAA
    CATAAGACTA TACCCGAGCG TGAAGCGCGC TCGATAATAA TGCAGGTTGT ATCTGCACTC
    AAGTATCTAA ATGAGATTAA GCCTCCAGTT ATCCACTACG ATCTGAAGCC CGGCAACATT
    CTGCTTACCG AGGGCAACGT CTGCGGCGAG ATTAAGATCA CCGACTTCGG TCTGTCAAAG
    GTGATGGACG ACGAGAATTA CAATCCCGAT CACGGCATGG ATCTGACCTC TCAGGGGGCG
    GGAACCTACT GGTATCTGCC ACCCGAGTGC TTTGTCGTGG GCAAAAATCC GCCGAAAATC
    TCCTCCAAAG TGGACGTATG GAGTGTGGGT GTTATCTTCT ACCAGTGTCT GTACGGCAAA
    AAGCCCTTCG GTCACAATCA GTCGCAGGCC ACGATTCTCG AGGAGAATAC GATCCTGAAG
    GCCACCGAAG TGCAGTTCTC CAACAAGCCA ACCGTTTCTA ACGAGGCCAA GAGTTTCATT
    CGGGGATGCT TGGCCTATCG CAAGGAGGAT CGCATGGATG TGTTCGCACT GGCCAGGCAC
    GAGTACATTC AGCCACCGAT ACCGAAACAT GGGCGCGGTT CGCTCAATCA GCAACAGCAG
    GCGCAACAAC AGCAGCAGCA ACAACAGCAA CAGCAGCAGC AACAGTCGTC GACGTCACAG
    GCCAATTCTA CAGGCCAGAC ATCTTTCTCT GCCCACATGT TTGGCAATAT GAATCAGTCG
    AGTTCGTCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096873.1
    CDS1..4392
    Translation

    Target ORF information:

    RefSeq Version NM_001103403.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant B, partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103403.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCCGCGAT TGCGATGCGA CGACGTTGCT GCCGTGCTGC TGCCCCTGAT GATGCTGTTA 
    TTGCTGTTGT TGCTGTTGTT GCGTCGTCTC GTGGTTGTCG TTGTCGGCCA CTTCATCATC
    GACAAGCAGC AAGAGCAAGA GCAAGAGCAG CAGCACCAGC AGCAGCAGCA GCAGCAGAGG
    CACAGGCTGT TGGCTAAAAC AAAGCAGAGG ACCCGGACCT CGGCCGACAA AAAGGACGAC
    TTGTGTCCTC ATCAATTCAG TTTCGGTTTC AGTCGCAATT CAAACGCCAT CAAAATCAAA
    ACGGCGCCCA GCTATCAACA ACATTTTCAT CATCAAAATC TTCACCACCA CCAACAACAA
    CATCAACAAC AACAACAACA ACAACATATA ACGCAAGTGC AACAACAACA ACAACAACAA
    CAGCAACTTT TGGTATTGCC ACAGCAGCAG CAACAGCAAC ATTTGCGAGT GTGCAACAAT
    CAAATCAATA ACAACAATAA CAGCAGTAAT AGCTGCCAAA ACAGCTATCA ATATAGTTAT
    AACAATAATA ATAACAACAC CAACGCCAAC AACAACAGCA ATCAGCAGCT GCAACAACAA
    ATTGTGAATA ACAACAACAA TCTCGAGGAC GATGTGTATT ATTTATTTTA TAAAGATTTT
    TATATGTCCC CCGGCGCCCA TTTGCAGATG TCCCCGCAGA ACACTTCGTC CCTAAGTCAA
    CACCATCCAC ATCAACAGCA ACAGTTACAA CCCCCACAGC AGCAACAACA GCATTTCCCT
    AACCATCACA GCGCCCAGCA ACAGTCGCAG CAGCAGCAGC AACAGGAGCA ACAGAATCCC
    CAGCAGCAGG CGCAACAGCA GCAGCAGATA CTCCCACATC AACATTTGCA GCACCTGCAC
    AAGCATCCGC ATCAGCTGCA ACTGCATCAG CAGCAGCAAC AACAACTCCA CCAGCAACAG
    CAGCAACACT TCCACCAGCA GTCGCTGCAA GGGCTGCATC AGGGTAGCAG CAATCCGGAT
    TCGAATATGA GCACTGGCTC CTCGCATAGC GAGAAGGATG TCAATGATAT GCTGAGTGGC
    GGTGCAGCAA CGCCAGGAGC TGCAGCAGCA GCGATTCAAC AGCAACATCC CGCCTTTGCG
    CCCACACTGG GAATGCAGCA ACCACCGCCG CCCCCACCTC AACACTCCAA TAATGGAGGC
    GAGATGGGCT ACTTGTCGGC AGGCACGACC ACGACGACGT CGGTGTTAAC GGTAGGCAAG
    CCTCGGACGC CAGCGGAGCG GAAACGGAAG CGAAAAATGC CTCCATGTGC CACTAGTGCG
    GATGAGGCGG GGAGTGGCGG TGGCTCTGGC GGAGCAGGAG CAACCGTTGT TAACAACAGC
    AGCCTGAAGG GCAAATCATT GGCCTTTCGT GATATGCCCA AGGTAAACAT GAGCCTGAAT
    CTGGGCGATC GTCTGGGAGG ATCTGCAGGA AGCGGAGTAG GAGCCGGTGG CGCCGGAAGC
    GGGGGAGGTG GCGCTGGTTC CGGTTCTGGA AGCGGTGGCG GCAAAAGCGC CCGCCTGATG
    CTGCCAGTCA GCGACAACAA GAAGATCAAC GACTATTTCA ATAAGCAGCA AACGGGCGTG
    GGCGTCGGTG TGCCAGGTGG TGCGGGAGGC AATACCGCTG GCCTTCGAGG ATCACATACG
    GGAGGTGGCA GCAAGTCACC CTCATCCGCC CAGCAGCAGC AAACGGCGGC ACAGCAGCAG
    GGAAGCGGTG TTGCGACGGG AGGCAGTGCA GGCGGTTCCG CTGGCAACCA GGTGCAAGTG
    CAAACGAGCA GCGCTTACGC CCTTTACCCA CCAGCTAGTC CCCAAACCCA GACGTCACAG
    CAACAGCAGC AGCAGCAACC GGGATCAGAC TTTCACTATG TCAACTCCAG CAAGGCGCAG
    CAACAACAGC AGCGTCAACA GCAACAGACT TCCAATCAAA TGGTTCCTCC ACACGTGGTC
    GTTGGCCTTG GTGGTCATCC ACTGAGCCTC GCGTCCATTC AGCAGCAGAC GCCCTTATCC
    CAGCAGCAAC AGCAGCAACA ACAGCAGCAG CAACAGCAGC AACTGGGACC ACCGACCACA
    TCGACGGCCT CCGTCGTGCC AACGCATCCG CATCAACTCG GATCCCTGGG AGTTGTTGGG
    ATGGTCGGTG TGGGTGTTGG CGTGGGCGTT GGAGTAAATG TGGGTGTGGG ACCACCACTG
    CCACCACCAC CGCCGATGGC CATGCCAGCG GCCATTATCA CTTATAGTAA GGCCACTCAA
    ACGGAGGTGT CGCTGCATGA ATTGCAGGAG CGCGAAGCGG AGCACGAATC GGGCAAGGTG
    AAGCTAGACG AGATGACACG GCTGTCCGAT GAACAAAAGT CCCAAATTGT TGGCAACCAG
    AAGACGATTG ACCAGCACAA GTGCCACATA GCCAAGTGTA TTGATGTGGT CAAGAAGCTG
    TTGAAGGAGA AGAGCAGCAT CGAGAAGAAG GAGGCGCGAC AGAAGTGCAT GCAGAATCGC
    CTCAGGCTCG GACAGTTTGT TACCCAACGA GTGGGCGCCA CATTCCAGGA GAACTGGACG
    GACGGCTATG CGTTCCAGGA GCTGAGTCGG CGGCAAGAAG AAATAACCGC TGAGCGTGAA
    GAGATAGATC GGCAGAAAAA GCAGCTGATG AAAAAGCGTC CGGCGGAGTC CGGACGCAAG
    CGCAACAACA ACAGTAACCA GAACAACCAG CAGCAGCAGC AACAGCAACA CCAGCAACAG
    CAGCAGCAAC AAAATTCCAA CTCGAACGAT TCCACGCAGC TGACGAGCGG AGTTGTTACC
    GGTCCAGGCA GTGATCGTGT GAGCGTAAGC GTCGACAGCG GATTGGGTGG CAATAATGCG
    GGCGCGATCG GTGGCGGAAC CGTTGGTGGT GGCGTTGGAG GTGGTGGTGT TGGAGGCGGT
    GGTGTCGGAG GCGGCGGTGG ACGTGGACTT TCTCGCAGCA ATTCGACGCA GGCCAATCAG
    GCTCAATTGC TGCACAACGG CGGTGGTGGT TCGGGCGGCA ATGTCGGCAA CTCGGGCGGC
    GTTGGCGACC GCTTGTCAGA TCGAGGAGGA GGAGGTGGCG GCATCGGCGG AAACGATAGC
    GGCAGCTGCT CGGACTCGGG CACTTTCCTG AAGCCAGACC CCGTATCGGG TGCCTACACA
    GCGCAGGAGT ATTACGAGTA CGATGAGATC CTCAAGTTGC GACAAAATGC CCTCAAAAAG
    GAGGACGCCG ACCTGCAGCT GGAGATGGAG AAGCTGGAGC GGGAGCGCAA TCTGCACATC
    CGAGAGCTCA AGCGGATTCT TAACGAGGAT CAGTCCCGCT TTAACAATCA TCCCGTGCTG
    AATGATCGCT ATCTTCTGTT GATGCTCCTG GGCAAGGGCG GCTTCTCAGA GGTCCACAAG
    GCCTTCGACC TGAAGGAGCA ACGCTATGTC GCATGTAAGG TGCACCAATT AAACAAGGAT
    TGGAAGGAGG ATAAGAAAGC TAATTATATC AAACACGCTT TGCGGGAATA CAACATTCAC
    AAGGCACTGG ATCATCCGCG GGTCGTCAAG CTATACGATG TCTTCGAGAT CGATGCGAAT
    TCCTTTTGCA CAGTGCTCGA ATACTGTGAT GGCCACGATC TGGACTTCTA TTTGAAGCAA
    CATAAGACTA TACCCGAGCG TGAAGCGCGC TCGATAATAA TGCAGGTTGT ATCTGCACTC
    AAGTATCTAA ATGAGATTAA GCCTCCAGTT ATCCACTACG ATCTGAAGCC CGGCAACATT
    CTGCTTACCG AGGGCAACGT CTGCGGCGAG ATTAAGATCA CCGACTTCGG TCTGTCAAAG
    GTGATGGACG ACGAGAATTA CAATCCCGAT CACGGCATGG ATCTGACCTC TCAGGGGGCG
    GGAACCTACT GGTATCTGCC ACCCGAGTGC TTTGTCGTGG GCAAAAATCC GCCGAAAATC
    TCCTCCAAAG TGGACGTATG GAGTGTGGGT GTTATCTTCT ACCAGTGTCT GTACGGCAAA
    AAGCCCTTCG GTCACAATCA GTCGCAGGCC ACGATTCTCG AGGAGAATAC GATCCTGAAG
    GCCACCGAAG TGCAGTTCTC CAACAAGCCA ACCGTTTCTA ACGAGGCCAA GAGTTTCATT
    CGGGGATGCT TGGCCTATCG CAAGGAGGAT CGCATGGATG TGTTCGCACT GGCCAGGCAC
    GAGTACATTC AGCCACCGAT ACCGAAACAT GGGCGCGGTT CGCTCAATCA GCAACAGCAG
    GCGCAACAAC AGCAGCAGCA ACAACAGCAA CAGCAGCAGC AACAGTCGTC GACGTCACAG
    GCCAATTCTA CAGGCCAGAC ATCTTTCTCT GCCCACATGT TTGGCAATAT GAATCAGTCG
    AGTTCGTCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02929
    Clone ID Related Accession (Same CDS sequence) NM_001144681.3
    Accession Version NM_001144681.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Tousled-like kinase, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_001144681.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTGTA CGCCGTCTCC TCTTTGCGGT TCATTCGCTC TAGCAATGCT CCAGCAACAA 
    CAGCAACAAC TGGAAATAAG AGAAGCACAG CAAAGGCAAC AACAGAAATC GCGTTTACAA
    CTAAATAATT ATTATAAAGA TATACCCCGA AATCAACATC ATATGCAAAA CTGTCAGGAT
    TTTCTACAAG TTCAGCAAAC CGTAGAAATA AACAAGTTGC ATTTTGTACA AAAAACACAG
    CAACAAGAAT GTTATATGCA ACAAATTCAC CATCATCAAC AACAGCAACA AAAGCAAATC
    GTTGATTATC AGCGTGTCAA TGAAAGGGCG CACATGCAAC CCACACAAAT ATTGCAAAGT
    CAGCAGAAAA TATCAGAATT GCAACAAATA CAACGTTCAG CAAGAAAACC AGAACTGTTG
    CAAATACCAG AAATACTACA AAAACAGCAA TTTCAAACAG CTCAACAAAA GCAAACGAAA
    AAGCAAGGTG CAAATACAGC AACGATAACA TCGTTTAACA GCAATAGCAA TAGCAACAAT
    AGTAGCAGCA GTTGCAAGAA CTACAATACC ACTAGTAACA GCAACAAAAA TAATATCGAC
    AATAATAGCA GCAATAGCAG CGGCCAAATA ATGTTAGCCT CTGCCAGCGC CTCTGCCTCC
    GCTCGGAAAC GCAGTCGCAG CACGAGTAAC GGCAGCAAAA GCAACAACAA AACTGCCAGT
    GGCCGCATAT TTACAAAAAG CATGTCCCCC GGCGCCCATT TGCAGATGTC CCCGCAGAAC
    ACTTCGTCCC TAAGTCAACA CCATCCACAT CAACAGCAAC AGTTACAACC CCCACAGCAG
    CAACAACAGC ATTTCCCTAA CCATCACAGC GCCCAGCAAC AGTCGCAGCA GCAGCAGCAA
    CAGGAGCAAC AGAATCCCCA GCAGCAGGCG CAACAGCAGC AGCAGATACT CCCACATCAA
    CATTTGCAGC ACCTGCACAA GCATCCGCAT CAGCTGCAAC TGCATCAGCA GCAGCAACAA
    CAACTCCACC AGCAACAGCA GCAACACTTC CACCAGCAGT CGCTGCAAGG GCTGCATCAG
    GGTAGCAGCA ATCCGGATTC GAATATGAGC ACTGGCTCCT CGCATAGCGA GAAGGATGTC
    AATGATATGC TGAGTGGCGG TGCAGCAACG CCAGGAGCTG CAGCAGCAGC GATTCAACAG
    CAACATCCCG CCTTTGCGCC CACACTGGGA ATGCAGCAAC CACCGCCGCC CCCACCTCAA
    CACTCCAATA ATGGAGGCGA GATGGGCTAC TTGTCGGCAG GCACGACCAC GACGACGTCG
    GTGTTAACGG TAGGCAAGCC TCGGACGCCA GCGGAGCGGA AACGGAAGCG AAAAATGCCT
    CCATGTGCCA CTAGTGCGGA TGAGGCGGGG AGTGGCGGTG GCTCTGGCGG AGCAGGAGCA
    ACCGTTGTTA ACAACAGCAG CCTGAAGGGC AAATCATTGG CCTTTCGTGA TATGCCCAAG
    GTAAACATGA GCCTGAATCT GGGCGATCGT CTGGGAGGAT CTGCAGGAAG CGGAGTAGGA
    GCCGGTGGCG CCGGAAGCGG GGGAGGTGGC GCTGGTTCCG GTTCTGGAAG CGGTGGCGGC
    AAAAGCGCCC GCCTGATGCT GCCAGTCAGC GACAACAAGA AGATCAACGA CTATTTCAAT
    AAGCAGCAAA CGGGCGTGGG CGTCGGTGTG CCAGGTGGTG CGGGAGGCAA TACCGCTGGC
    CTTCGAGGAT CACATACGGG AGGTGGCAGC AAGTCACCCT CATCCGCCCA GCAGCAGCAA
    ACGGCGGCAC AGCAGCAGGG AAGCGGTGTT GCGACGGGAG GCAGTGCAGG CGGTTCCGCT
    GGCAACCAGG TGCAAGTGCA AACGAGCAGC GCTTACGCCC TTTACCCACC AGCTAGTCCC
    CAAACCCAGA CGTCACAGCA ACAGCAGCAG CAGCAACCGG GATCAGACTT TCACTATGTC
    AACTCCAGCA AGGCGCAGCA ACAACAGCAG CGTCAACAGC AACAGACTTC CAATCAAATG
    GTTCCTCCAC ACGTGGTCGT TGGCCTTGGT GGTCATCCAC TGAGCCTCGC GTCCATTCAG
    CAGCAGACGC CCTTATCCCA GCAGCAACAG CAGCAACAAC AGCAGCAGCA ACAGCAGCAA
    CTGGGACCAC CGACCACATC GACGGCCTCC GTCGTGCCAA CGCATCCGCA TCAACTCGGA
    TCCCTGGGAG TTGTTGGGAT GGTCGGTGTG GGTGTTGGCG TGGGCGTTGG AGTAAATGTG
    GGTGTGGGAC CACCACTGCC ACCACCACCG CCGATGGCCA TGCCAGCGGC CATTATCACT
    TATAGTAAGG CCACTCAAAC GGAGGTGTCG CTGCATGAAT TGCAGGAGCG CGAAGCGGAG
    CACGAATCGG GCAAGGTGAA GCTAGACGAG ATGACACGGC TGTCCGATGA ACAAAAGTCC
    CAAATTGTTG GCAACCAGAA GACGATTGAC CAGCACAAGT GCCACATAGC CAAGTGTATT
    GATGTGGTCA AGAAGCTGTT GAAGGAGAAG AGCAGCATCG AGAAGAAGGA GGCGCGACAG
    AAGTGCATGC AGAATCGCCT CAGGCTCGGA CAGTTTGTTA CCCAACGAGT GGGCGCCACA
    TTCCAGGAGA ACTGGACGGA CGGCTATGCG TTCCAGGAGC TGAGTCGGCG GCAAGAAGAA
    ATAACCGCTG AGCGTGAAGA GATAGATCGG CAGAAAAAGC AGCTGATGAA AAAGCGTCCG
    GCGGAGTCCG GACGCAAGCG CAACAACAAC AGTAACCAGA ACAACCAGCA GCAGCAGCAA
    CAGCAACACC AGCAACAGCA GCAGCAACAA AATTCCAACT CGAACGATTC CACGCAGCTG
    ACGAGCGGAG TTGTTACCGG TCCAGGCAGT GATCGTGTGA GCGTAAGCGT CGACAGCGGA
    TTGGGTGGCA ATAATGCGGG CGCGATCGGT GGCGGAACCG TTGGTGGTGG CGTTGGAGGT
    GGTGGTGTTG GAGGCGGTGG TGTCGGAGGC GGCGGTGGAC GTGGACTTTC TCGCAGCAAT
    TCGACGCAGG CCAATCAGGC TCAATTGCTG CACAACGGCG GTGGTGGTTC GGGCGGCAAT
    GTCGGCAACT CGGGCGGCGT TGGCGACCGC TTGTCAGATC GAGGAGGAGG AGGTGGCGGC
    ATCGGCGGAA ACGATAGCGG CAGCTGCTCG GACTCGGGCA CTTTCCTGAA GCCAGACCCC
    GTATCGGGTG CCTACACAGC GCAGGAGTAT TACGAGTACG ATGAGATCCT CAAGTTGCGA
    CAAAATGCCC TCAAAAAGGA GGACGCCGAC CTGCAGCTGG AGATGGAGAA GCTGGAGCGG
    GAGCGCAATC TGCACATCCG AGAGCTCAAG CGGATTCTTA ACGAGGATCA GTCCCGCTTT
    AACAATCATC CCGTGCTGAA TGATCGCTAT CTTCTGTTGA TGCTCCTGGG CAAGGGCGGC
    TTCTCAGAGG TCCACAAGGC CTTCGACCTG AAGGAGCAAC GCTATGTCGC ATGTAAGGTG
    CACCAATTAA ACAAGGATTG GAAGGAGGAT AAGAAAGCTA ATTATATCAA ACACGCTTTG
    CGGGAATACA ACATTCACAA GGCACTGGAT CATCCGCGGG TCGTCAAGCT ATACGATGTC
    TTCGAGATCG ATGCGAATTC CTTTTGCACA GTGCTCGAAT ACTGTGATGG CCACGATCTG
    GACTTCTATT TGAAGCAACA TAAGACTATA CCCGAGCGTG AAGCGCGCTC GATAATAATG
    CAGGTTGTAT CTGCACTCAA GTATCTAAAT GAGATTAAGC CTCCAGTTAT CCACTACGAT
    CTGAAGCCCG GCAACATTCT GCTTACCGAG GGCAACGTCT GCGGCGAGAT TAAGATCACC
    GACTTCGGTC TGTCAAAGGT GATGGACGAC GAGAATTACA ATCCCGATCA CGGCATGGAT
    CTGACCTCTC AGGGGGCGGG AACCTACTGG TATCTGCCAC CCGAGTGCTT TGTCGTGGGC
    AAAAATCCGC CGAAAATCTC CTCCAAAGTG GACGTATGGA GTGTGGGTGT TATCTTCTAC
    CAGTGTCTGT ACGGCAAAAA GCCCTTCGGT CACAATCAGT CGCAGGCCAC GATTCTCGAG
    GAGAATACGA TCCTGAAGGC CACCGAAGTG CAGTTCTCCA ACAAGCCAAC CGTTTCTAAC
    GAGGCCAAGA GTTTCATTCG GGGATGCTTG GCCTATCGCA AGGAGGATCG CATGGATGTG
    TTCGCACTGG CCAGGCACGA GTACATTCAG CCACCGATAC CGAAACATGG GCGCGGTTCG
    CTCAATCAGC AACAGCAGGC GCAACAACAG CAGCAGCAAC AACAGCAACA GCAGCAGCAA
    CAGTCGTCGA CGTCACAGGC CAATTCTACA GGCCAGACAT CTTTCTCTGC CCACATGTTT
    GGCAATATGA ATCAGTCGAG TTCGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138153.1
    CDS1201..5670
    Translation

    Target ORF information:

    RefSeq Version NM_001144681.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144681.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTGTA CGCCGTCTCC TCTTTGCGGT TCATTCGCTC TAGCAATGCT CCAGCAACAA 
    CAGCAACAAC TGGAAATAAG AGAAGCACAG CAAAGGCAAC AACAGAAATC GCGTTTACAA
    CTAAATAATT ATTATAAAGA TATACCCCGA AATCAACATC ATATGCAAAA CTGTCAGGAT
    TTTCTACAAG TTCAGCAAAC CGTAGAAATA AACAAGTTGC ATTTTGTACA AAAAACACAG
    CAACAAGAAT GTTATATGCA ACAAATTCAC CATCATCAAC AACAGCAACA AAAGCAAATC
    GTTGATTATC AGCGTGTCAA TGAAAGGGCG CACATGCAAC CCACACAAAT ATTGCAAAGT
    CAGCAGAAAA TATCAGAATT GCAACAAATA CAACGTTCAG CAAGAAAACC AGAACTGTTG
    CAAATACCAG AAATACTACA AAAACAGCAA TTTCAAACAG CTCAACAAAA GCAAACGAAA
    AAGCAAGGTG CAAATACAGC AACGATAACA TCGTTTAACA GCAATAGCAA TAGCAACAAT
    AGTAGCAGCA GTTGCAAGAA CTACAATACC ACTAGTAACA GCAACAAAAA TAATATCGAC
    AATAATAGCA GCAATAGCAG CGGCCAAATA ATGTTAGCCT CTGCCAGCGC CTCTGCCTCC
    GCTCGGAAAC GCAGTCGCAG CACGAGTAAC GGCAGCAAAA GCAACAACAA AACTGCCAGT
    GGCCGCATAT TTACAAAAAG CATGTCCCCC GGCGCCCATT TGCAGATGTC CCCGCAGAAC
    ACTTCGTCCC TAAGTCAACA CCATCCACAT CAACAGCAAC AGTTACAACC CCCACAGCAG
    CAACAACAGC ATTTCCCTAA CCATCACAGC GCCCAGCAAC AGTCGCAGCA GCAGCAGCAA
    CAGGAGCAAC AGAATCCCCA GCAGCAGGCG CAACAGCAGC AGCAGATACT CCCACATCAA
    CATTTGCAGC ACCTGCACAA GCATCCGCAT CAGCTGCAAC TGCATCAGCA GCAGCAACAA
    CAACTCCACC AGCAACAGCA GCAACACTTC CACCAGCAGT CGCTGCAAGG GCTGCATCAG
    GGTAGCAGCA ATCCGGATTC GAATATGAGC ACTGGCTCCT CGCATAGCGA GAAGGATGTC
    AATGATATGC TGAGTGGCGG TGCAGCAACG CCAGGAGCTG CAGCAGCAGC GATTCAACAG
    CAACATCCCG CCTTTGCGCC CACACTGGGA ATGCAGCAAC CACCGCCGCC CCCACCTCAA
    CACTCCAATA ATGGAGGCGA GATGGGCTAC TTGTCGGCAG GCACGACCAC GACGACGTCG
    GTGTTAACGG TAGGCAAGCC TCGGACGCCA GCGGAGCGGA AACGGAAGCG AAAAATGCCT
    CCATGTGCCA CTAGTGCGGA TGAGGCGGGG AGTGGCGGTG GCTCTGGCGG AGCAGGAGCA
    ACCGTTGTTA ACAACAGCAG CCTGAAGGGC AAATCATTGG CCTTTCGTGA TATGCCCAAG
    GTAAACATGA GCCTGAATCT GGGCGATCGT CTGGGAGGAT CTGCAGGAAG CGGAGTAGGA
    GCCGGTGGCG CCGGAAGCGG GGGAGGTGGC GCTGGTTCCG GTTCTGGAAG CGGTGGCGGC
    AAAAGCGCCC GCCTGATGCT GCCAGTCAGC GACAACAAGA AGATCAACGA CTATTTCAAT
    AAGCAGCAAA CGGGCGTGGG CGTCGGTGTG CCAGGTGGTG CGGGAGGCAA TACCGCTGGC
    CTTCGAGGAT CACATACGGG AGGTGGCAGC AAGTCACCCT CATCCGCCCA GCAGCAGCAA
    ACGGCGGCAC AGCAGCAGGG AAGCGGTGTT GCGACGGGAG GCAGTGCAGG CGGTTCCGCT
    GGCAACCAGG TGCAAGTGCA AACGAGCAGC GCTTACGCCC TTTACCCACC AGCTAGTCCC
    CAAACCCAGA CGTCACAGCA ACAGCAGCAG CAGCAACCGG GATCAGACTT TCACTATGTC
    AACTCCAGCA AGGCGCAGCA ACAACAGCAG CGTCAACAGC AACAGACTTC CAATCAAATG
    GTTCCTCCAC ACGTGGTCGT TGGCCTTGGT GGTCATCCAC TGAGCCTCGC GTCCATTCAG
    CAGCAGACGC CCTTATCCCA GCAGCAACAG CAGCAACAAC AGCAGCAGCA ACAGCAGCAA
    CTGGGACCAC CGACCACATC GACGGCCTCC GTCGTGCCAA CGCATCCGCA TCAACTCGGA
    TCCCTGGGAG TTGTTGGGAT GGTCGGTGTG GGTGTTGGCG TGGGCGTTGG AGTAAATGTG
    GGTGTGGGAC CACCACTGCC ACCACCACCG CCGATGGCCA TGCCAGCGGC CATTATCACT
    TATAGTAAGG CCACTCAAAC GGAGGTGTCG CTGCATGAAT TGCAGGAGCG CGAAGCGGAG
    CACGAATCGG GCAAGGTGAA GCTAGACGAG ATGACACGGC TGTCCGATGA ACAAAAGTCC
    CAAATTGTTG GCAACCAGAA GACGATTGAC CAGCACAAGT GCCACATAGC CAAGTGTATT
    GATGTGGTCA AGAAGCTGTT GAAGGAGAAG AGCAGCATCG AGAAGAAGGA GGCGCGACAG
    AAGTGCATGC AGAATCGCCT CAGGCTCGGA CAGTTTGTTA CCCAACGAGT GGGCGCCACA
    TTCCAGGAGA ACTGGACGGA CGGCTATGCG TTCCAGGAGC TGAGTCGGCG GCAAGAAGAA
    ATAACCGCTG AGCGTGAAGA GATAGATCGG CAGAAAAAGC AGCTGATGAA AAAGCGTCCG
    GCGGAGTCCG GACGCAAGCG CAACAACAAC AGTAACCAGA ACAACCAGCA GCAGCAGCAA
    CAGCAACACC AGCAACAGCA GCAGCAACAA AATTCCAACT CGAACGATTC CACGCAGCTG
    ACGAGCGGAG TTGTTACCGG TCCAGGCAGT GATCGTGTGA GCGTAAGCGT CGACAGCGGA
    TTGGGTGGCA ATAATGCGGG CGCGATCGGT GGCGGAACCG TTGGTGGTGG CGTTGGAGGT
    GGTGGTGTTG GAGGCGGTGG TGTCGGAGGC GGCGGTGGAC GTGGACTTTC TCGCAGCAAT
    TCGACGCAGG CCAATCAGGC TCAATTGCTG CACAACGGCG GTGGTGGTTC GGGCGGCAAT
    GTCGGCAACT CGGGCGGCGT TGGCGACCGC TTGTCAGATC GAGGAGGAGG AGGTGGCGGC
    ATCGGCGGAA ACGATAGCGG CAGCTGCTCG GACTCGGGCA CTTTCCTGAA GCCAGACCCC
    GTATCGGGTG CCTACACAGC GCAGGAGTAT TACGAGTACG ATGAGATCCT CAAGTTGCGA
    CAAAATGCCC TCAAAAAGGA GGACGCCGAC CTGCAGCTGG AGATGGAGAA GCTGGAGCGG
    GAGCGCAATC TGCACATCCG AGAGCTCAAG CGGATTCTTA ACGAGGATCA GTCCCGCTTT
    AACAATCATC CCGTGCTGAA TGATCGCTAT CTTCTGTTGA TGCTCCTGGG CAAGGGCGGC
    TTCTCAGAGG TCCACAAGGC CTTCGACCTG AAGGAGCAAC GCTATGTCGC ATGTAAGGTG
    CACCAATTAA ACAAGGATTG GAAGGAGGAT AAGAAAGCTA ATTATATCAA ACACGCTTTG
    CGGGAATACA ACATTCACAA GGCACTGGAT CATCCGCGGG TCGTCAAGCT ATACGATGTC
    TTCGAGATCG ATGCGAATTC CTTTTGCACA GTGCTCGAAT ACTGTGATGG CCACGATCTG
    GACTTCTATT TGAAGCAACA TAAGACTATA CCCGAGCGTG AAGCGCGCTC GATAATAATG
    CAGGTTGTAT CTGCACTCAA GTATCTAAAT GAGATTAAGC CTCCAGTTAT CCACTACGAT
    CTGAAGCCCG GCAACATTCT GCTTACCGAG GGCAACGTCT GCGGCGAGAT TAAGATCACC
    GACTTCGGTC TGTCAAAGGT GATGGACGAC GAGAATTACA ATCCCGATCA CGGCATGGAT
    CTGACCTCTC AGGGGGCGGG AACCTACTGG TATCTGCCAC CCGAGTGCTT TGTCGTGGGC
    AAAAATCCGC CGAAAATCTC CTCCAAAGTG GACGTATGGA GTGTGGGTGT TATCTTCTAC
    CAGTGTCTGT ACGGCAAAAA GCCCTTCGGT CACAATCAGT CGCAGGCCAC GATTCTCGAG
    GAGAATACGA TCCTGAAGGC CACCGAAGTG CAGTTCTCCA ACAAGCCAAC CGTTTCTAAC
    GAGGCCAAGA GTTTCATTCG GGGATGCTTG GCCTATCGCA AGGAGGATCG CATGGATGTG
    TTCGCACTGG CCAGGCACGA GTACATTCAG CCACCGATAC CGAAACATGG GCGCGGTTCG
    CTCAATCAGC AACAGCAGGC GCAACAACAG CAGCAGCAAC AACAGCAACA GCAGCAGCAA
    CAGTCGTCGA CGTCACAGGC CAATTCTACA GGCCAGACAT CTTTCTCTGC CCACATGTTT
    GGCAATATGA ATCAGTCGAG TTCGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02930
    Clone ID Related Accession (Same CDS sequence) NM_130717.5
    Accession Version NM_130717.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Tousled-like kinase, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_130717.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTGTGTGC AGAAAAATAT GCGGCGCTTA AAAAAGATGT CCCCCGGCGC CCATTTGCAG 
    ATGTCCCCGC AGAACACTTC GTCCCTAAGT CAACACCATC CACATCAACA GCAACAGTTA
    CAACCCCCAC AGCAGCAACA ACAGCATTTC CCTAACCATC ACAGCGCCCA GCAACAGTCG
    CAGCAGCAGC AGCAACAGGA GCAACAGAAT CCCCAGCAGC AGGCGCAACA GCAGCAGCAG
    ATACTCCCAC ATCAACATTT GCAGCACCTG CACAAGCATC CGCATCAGCT GCAACTGCAT
    CAGCAGCAGC AACAACAACT CCACCAGCAA CAGCAGCAAC ACTTCCACCA GCAGTCGCTG
    CAAGGGCTGC ATCAGGGTAG CAGCAATCCG GATTCGAATA TGAGCACTGG CTCCTCGCAT
    AGCGAGAAGG ATGTCAATGA TATGCTGAGT GGCGGTGCAG CAACGCCAGG AGCTGCAGCA
    GCAGCGATTC AACAGCAACA TCCCGCCTTT GCGCCCACAC TGGGAATGCA GCAACCACCG
    CCGCCCCCAC CTCAACACTC CAATAATGGA GGCGAGATGG GCTACTTGTC GGCAGGCACG
    ACCACGACGA CGTCGGTGTT AACGGTAGGC AAGCCTCGGA CGCCAGCGGA GCGGAAACGG
    AAGCGAAAAA TGCCTCCATG TGCCACTAGT GCGGATGAGG CGGGGAGTGG CGGTGGCTCT
    GGCGGAGCAG GAGCAACCGT TGTTAACAAC AGCAGCCTGA AGGGCAAATC ATTGGCCTTT
    CGTGATATGC CCAAGGTAAA CATGAGCCTG AATCTGGGCG ATCGTCTGGG AGGATCTGCA
    GGAAGCGGAG TAGGAGCCGG TGGCGCCGGA AGCGGGGGAG GTGGCGCTGG TTCCGGTTCT
    GGAAGCGGTG GCGGCAAAAG CGCCCGCCTG ATGCTGCCAG TCAGCGACAA CAAGAAGATC
    AACGACTATT TCAATAAGCA GCAAACGGGC GTGGGCGTCG GTGTGCCAGG TGGTGCGGGA
    GGCAATACCG CTGGCCTTCG AGGATCACAT ACGGGAGGTG GCAGCAAGTC ACCCTCATCC
    GCCCAGCAGC AGCAAACGGC GGCACAGCAG CAGGGAAGCG GTGTTGCGAC GGGAGGCAGT
    GCAGGCGGTT CCGCTGGCAA CCAGGTGCAA GTGCAAACGA GCAGCGCTTA CGCCCTTTAC
    CCACCAGCTA GTCCCCAAAC CCAGACGTCA CAGCAACAGC AGCAGCAGCA ACCGGGATCA
    GACTTTCACT ATGTCAACTC CAGCAAGGCG CAGCAACAAC AGCAGCGTCA ACAGCAACAG
    ACTTCCAATC AAATGGTTCC TCCACACGTG GTCGTTGGCC TTGGTGGTCA TCCACTGAGC
    CTCGCGTCCA TTCAGCAGCA GACGCCCTTA TCCCAGCAGC AACAGCAGCA ACAACAGCAG
    CAGCAACAGC AGCAACTGGG ACCACCGACC ACATCGACGG CCTCCGTCGT GCCAACGCAT
    CCGCATCAAC TCGGATCCCT GGGAGTTGTT GGGATGGTCG GTGTGGGTGT TGGCGTGGGC
    GTTGGAGTAA ATGTGGGTGT GGGACCACCA CTGCCACCAC CACCGCCGAT GGCCATGCCA
    GCGGCCATTA TCACTTATAG TAAGGCCACT CAAACGGAGG TGTCGCTGCA TGAATTGCAG
    GAGCGCGAAG CGGAGCACGA ATCGGGCAAG GTGAAGCTAG ACGAGATGAC ACGGCTGTCC
    GATGAACAAA AGTCCCAAAT TGTTGGCAAC CAGAAGACGA TTGACCAGCA CAAGTGCCAC
    ATAGCCAAGT GTATTGATGT GGTCAAGAAG CTGTTGAAGG AGAAGAGCAG CATCGAGAAG
    AAGGAGGCGC GACAGAAGTG CATGCAGAAT CGCCTCAGGC TCGGACAGTT TGTTACCCAA
    CGAGTGGGCG CCACATTCCA GGAGAACTGG ACGGACGGCT ATGCGTTCCA GGAGCTGAGT
    CGGCGGCAAG AAGAAATAAC CGCTGAGCGT GAAGAGATAG ATCGGCAGAA AAAGCAGCTG
    ATGAAAAAGC GTCCGGCGGA GTCCGGACGC AAGCGCAACA ACAACAGTAA CCAGAACAAC
    CAGCAGCAGC AGCAACAGCA ACACCAGCAA CAGCAGCAGC AACAAAATTC CAACTCGAAC
    GATTCCACGC AGCTGACGAG CGGAGTTGTT ACCGGTCCAG GCAGTGATCG TGTGAGCGTA
    AGCGTCGACA GCGGATTGGG TGGCAATAAT GCGGGCGCGA TCGGTGGCGG AACCGTTGGT
    GGTGGCGTTG GAGGTGGTGG TGTTGGAGGC GGTGGTGTCG GAGGCGGCGG TGGACGTGGA
    CTTTCTCGCA GCAATTCGAC GCAGGCCAAT CAGGCTCAAT TGCTGCACAA CGGCGGTGGT
    GGTTCGGGCG GCAATGTCGG CAACTCGGGC GGCGTTGGCG ACCGCTTGTC AGATCGAGGA
    GGAGGAGGTG GCGGCATCGG CGGAAACGAT AGCGGCAGCT GCTCGGACTC GGGCACTTTC
    CTGAAGCCAG ACCCCGTATC GGGTGCCTAC ACAGCGCAGG AGTATTACGA GTACGATGAG
    ATCCTCAAGT TGCGACAAAA TGCCCTCAAA AAGGAGGACG CCGACCTGCA GCTGGAGATG
    GAGAAGCTGG AGCGGGAGCG CAATCTGCAC ATCCGAGAGC TCAAGCGGAT TCTTAACGAG
    GATCAGTCCC GCTTTAACAA TCATCCCGTG CTGAATGATC GCTATCTTCT GTTGATGCTC
    CTGGGCAAGG GCGGCTTCTC AGAGGTCCAC AAGGCCTTCG ACCTGAAGGA GCAACGCTAT
    GTCGCATGTA AGGTGCACCA ATTAAACAAG GATTGGAAGG AGGATAAGAA AGCTAATTAT
    ATCAAACACG CTTTGCGGGA ATACAACATT CACAAGGCAC TGGATCATCC GCGGGTCGTC
    AAGCTATACG ATGTCTTCGA GATCGATGCG AATTCCTTTT GCACAGTGCT CGAATACTGT
    GATGGCCACG ATCTGGACTT CTATTTGAAG CAACATAAGA CTATACCCGA GCGTGAAGCG
    CGCTCGATAA TAATGCAGGT TGTATCTGCA CTCAAGTATC TAAATGAGAT TAAGCCTCCA
    GTTATCCACT ACGATCTGAA GCCCGGCAAC ATTCTGCTTA CCGAGGGCAA CGTCTGCGGC
    GAGATTAAGA TCACCGACTT CGGTCTGTCA AAGGTGATGG ACGACGAGAA TTACAATCCC
    GATCACGGCA TGGATCTGAC CTCTCAGGGG GCGGGAACCT ACTGGTATCT GCCACCCGAG
    TGCTTTGTCG TGGGCAAAAA TCCGCCGAAA ATCTCCTCCA AAGTGGACGT ATGGAGTGTG
    GGTGTTATCT TCTACCAGTG TCTGTACGGC AAAAAGCCCT TCGGTCACAA TCAGTCGCAG
    GCCACGATTC TCGAGGAGAA TACGATCCTG AAGGCCACCG AAGTGCAGTT CTCCAACAAG
    CCAACCGTTT CTAACGAGGC CAAGAGTTTC ATTCGGGGAT GCTTGGCCTA TCGCAAGGAG
    GATCGCATGG ATGTGTTCGC ACTGGCCAGG CACGAGTACA TTCAGCCACC GATACCGAAA
    CATGGGCGCG GTTCGCTCAA TCAGCAACAG CAGGCGCAAC AACAGCAGCA GCAACAACAG
    CAACAGCAGC AGCAACAGTC GTCGACGTCA CAGGCCAATT CTACAGGCCA GACATCTTTC
    TCTGCCCACA TGTTTGGCAA TATGAATCAG TCGAGTTCGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_570073.3
    CDS512..4276
    Translation

    Target ORF information:

    RefSeq Version NM_130717.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Tousled-like kinase (Tlk), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_130717.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTGTGTGC AGAAAAATAT GCGGCGCTTA AAAAAGATGT CCCCCGGCGC CCATTTGCAG 
    ATGTCCCCGC AGAACACTTC GTCCCTAAGT CAACACCATC CACATCAACA GCAACAGTTA
    CAACCCCCAC AGCAGCAACA ACAGCATTTC CCTAACCATC ACAGCGCCCA GCAACAGTCG
    CAGCAGCAGC AGCAACAGGA GCAACAGAAT CCCCAGCAGC AGGCGCAACA GCAGCAGCAG
    ATACTCCCAC ATCAACATTT GCAGCACCTG CACAAGCATC CGCATCAGCT GCAACTGCAT
    CAGCAGCAGC AACAACAACT CCACCAGCAA CAGCAGCAAC ACTTCCACCA GCAGTCGCTG
    CAAGGGCTGC ATCAGGGTAG CAGCAATCCG GATTCGAATA TGAGCACTGG CTCCTCGCAT
    AGCGAGAAGG ATGTCAATGA TATGCTGAGT GGCGGTGCAG CAACGCCAGG AGCTGCAGCA
    GCAGCGATTC AACAGCAACA TCCCGCCTTT GCGCCCACAC TGGGAATGCA GCAACCACCG
    CCGCCCCCAC CTCAACACTC CAATAATGGA GGCGAGATGG GCTACTTGTC GGCAGGCACG
    ACCACGACGA CGTCGGTGTT AACGGTAGGC AAGCCTCGGA CGCCAGCGGA GCGGAAACGG
    AAGCGAAAAA TGCCTCCATG TGCCACTAGT GCGGATGAGG CGGGGAGTGG CGGTGGCTCT
    GGCGGAGCAG GAGCAACCGT TGTTAACAAC AGCAGCCTGA AGGGCAAATC ATTGGCCTTT
    CGTGATATGC CCAAGGTAAA CATGAGCCTG AATCTGGGCG ATCGTCTGGG AGGATCTGCA
    GGAAGCGGAG TAGGAGCCGG TGGCGCCGGA AGCGGGGGAG GTGGCGCTGG TTCCGGTTCT
    GGAAGCGGTG GCGGCAAAAG CGCCCGCCTG ATGCTGCCAG TCAGCGACAA CAAGAAGATC
    AACGACTATT TCAATAAGCA GCAAACGGGC GTGGGCGTCG GTGTGCCAGG TGGTGCGGGA
    GGCAATACCG CTGGCCTTCG AGGATCACAT ACGGGAGGTG GCAGCAAGTC ACCCTCATCC
    GCCCAGCAGC AGCAAACGGC GGCACAGCAG CAGGGAAGCG GTGTTGCGAC GGGAGGCAGT
    GCAGGCGGTT CCGCTGGCAA CCAGGTGCAA GTGCAAACGA GCAGCGCTTA CGCCCTTTAC
    CCACCAGCTA GTCCCCAAAC CCAGACGTCA CAGCAACAGC AGCAGCAGCA ACCGGGATCA
    GACTTTCACT ATGTCAACTC CAGCAAGGCG CAGCAACAAC AGCAGCGTCA ACAGCAACAG
    ACTTCCAATC AAATGGTTCC TCCACACGTG GTCGTTGGCC TTGGTGGTCA TCCACTGAGC
    CTCGCGTCCA TTCAGCAGCA GACGCCCTTA TCCCAGCAGC AACAGCAGCA ACAACAGCAG
    CAGCAACAGC AGCAACTGGG ACCACCGACC ACATCGACGG CCTCCGTCGT GCCAACGCAT
    CCGCATCAAC TCGGATCCCT GGGAGTTGTT GGGATGGTCG GTGTGGGTGT TGGCGTGGGC
    GTTGGAGTAA ATGTGGGTGT GGGACCACCA CTGCCACCAC CACCGCCGAT GGCCATGCCA
    GCGGCCATTA TCACTTATAG TAAGGCCACT CAAACGGAGG TGTCGCTGCA TGAATTGCAG
    GAGCGCGAAG CGGAGCACGA ATCGGGCAAG GTGAAGCTAG ACGAGATGAC ACGGCTGTCC
    GATGAACAAA AGTCCCAAAT TGTTGGCAAC CAGAAGACGA TTGACCAGCA CAAGTGCCAC
    ATAGCCAAGT GTATTGATGT GGTCAAGAAG CTGTTGAAGG AGAAGAGCAG CATCGAGAAG
    AAGGAGGCGC GACAGAAGTG CATGCAGAAT CGCCTCAGGC TCGGACAGTT TGTTACCCAA
    CGAGTGGGCG CCACATTCCA GGAGAACTGG ACGGACGGCT ATGCGTTCCA GGAGCTGAGT
    CGGCGGCAAG AAGAAATAAC CGCTGAGCGT GAAGAGATAG ATCGGCAGAA AAAGCAGCTG
    ATGAAAAAGC GTCCGGCGGA GTCCGGACGC AAGCGCAACA ACAACAGTAA CCAGAACAAC
    CAGCAGCAGC AGCAACAGCA ACACCAGCAA CAGCAGCAGC AACAAAATTC CAACTCGAAC
    GATTCCACGC AGCTGACGAG CGGAGTTGTT ACCGGTCCAG GCAGTGATCG TGTGAGCGTA
    AGCGTCGACA GCGGATTGGG TGGCAATAAT GCGGGCGCGA TCGGTGGCGG AACCGTTGGT
    GGTGGCGTTG GAGGTGGTGG TGTTGGAGGC GGTGGTGTCG GAGGCGGCGG TGGACGTGGA
    CTTTCTCGCA GCAATTCGAC GCAGGCCAAT CAGGCTCAAT TGCTGCACAA CGGCGGTGGT
    GGTTCGGGCG GCAATGTCGG CAACTCGGGC GGCGTTGGCG ACCGCTTGTC AGATCGAGGA
    GGAGGAGGTG GCGGCATCGG CGGAAACGAT AGCGGCAGCT GCTCGGACTC GGGCACTTTC
    CTGAAGCCAG ACCCCGTATC GGGTGCCTAC ACAGCGCAGG AGTATTACGA GTACGATGAG
    ATCCTCAAGT TGCGACAAAA TGCCCTCAAA AAGGAGGACG CCGACCTGCA GCTGGAGATG
    GAGAAGCTGG AGCGGGAGCG CAATCTGCAC ATCCGAGAGC TCAAGCGGAT TCTTAACGAG
    GATCAGTCCC GCTTTAACAA TCATCCCGTG CTGAATGATC GCTATCTTCT GTTGATGCTC
    CTGGGCAAGG GCGGCTTCTC AGAGGTCCAC AAGGCCTTCG ACCTGAAGGA GCAACGCTAT
    GTCGCATGTA AGGTGCACCA ATTAAACAAG GATTGGAAGG AGGATAAGAA AGCTAATTAT
    ATCAAACACG CTTTGCGGGA ATACAACATT CACAAGGCAC TGGATCATCC GCGGGTCGTC
    AAGCTATACG ATGTCTTCGA GATCGATGCG AATTCCTTTT GCACAGTGCT CGAATACTGT
    GATGGCCACG ATCTGGACTT CTATTTGAAG CAACATAAGA CTATACCCGA GCGTGAAGCG
    CGCTCGATAA TAATGCAGGT TGTATCTGCA CTCAAGTATC TAAATGAGAT TAAGCCTCCA
    GTTATCCACT ACGATCTGAA GCCCGGCAAC ATTCTGCTTA CCGAGGGCAA CGTCTGCGGC
    GAGATTAAGA TCACCGACTT CGGTCTGTCA AAGGTGATGG ACGACGAGAA TTACAATCCC
    GATCACGGCA TGGATCTGAC CTCTCAGGGG GCGGGAACCT ACTGGTATCT GCCACCCGAG
    TGCTTTGTCG TGGGCAAAAA TCCGCCGAAA ATCTCCTCCA AAGTGGACGT ATGGAGTGTG
    GGTGTTATCT TCTACCAGTG TCTGTACGGC AAAAAGCCCT TCGGTCACAA TCAGTCGCAG
    GCCACGATTC TCGAGGAGAA TACGATCCTG AAGGCCACCG AAGTGCAGTT CTCCAACAAG
    CCAACCGTTT CTAACGAGGC CAAGAGTTTC ATTCGGGGAT GCTTGGCCTA TCGCAAGGAG
    GATCGCATGG ATGTGTTCGC ACTGGCCAGG CACGAGTACA TTCAGCCACC GATACCGAAA
    CATGGGCGCG GTTCGCTCAA TCAGCAACAG CAGGCGCAAC AACAGCAGCA GCAACAACAG
    CAACAGCAGC AGCAACAGTC GTCGACGTCA CAGGCCAATT CTACAGGCCA GACATCTTTC
    TCTGCCCACA TGTTTGGCAA TATGAATCAG TCGAGTTCGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.