emb-9 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	emb-9
Entrez Gene ID	176314
Full Name	Collagen alpha-1(IV) chain
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001027491.4	NP_001022662.2	Collagen alpha-1(IV) chain
NM_001027492.4	NP_001022663.1	Collagen alpha-1(IV) chain
NM_001027492.5	NP_001022663.1	Collagen alpha-1(IV) chain
NM_001027491.5	NP_001022662.2	Collagen alpha-1(IV) chain

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL4A2	XP_001136859.1
Canis lupus familiaris (dog)	COL4A2	XP_534183.3
Rattus norvegicus (Norway rat)	Col4a2	XP_006253538.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009200	XP_319976.3
	COL4A2	XP_002800878.1
Drosophila melanogaster (fruit fly)	Cg25C	NP_723046.1
Caenorhabditis elegans (roundworm)	emb-9	NP_001022662.1
Bos taurus (cattle)	COL4A2	XP_002692057.2
Mus musculus (house mouse)	Col4a2	NP_034062.3
Gallus gallus (chicken)	COL4A2	NP_001155862.1
Danio rerio (zebrafish)	col4a2	XP_687811.6
Caenorhabditis elegans (roundworm)	let-2	NP_510663.1
Xenopus tropicalis (tropical clawed frog)	col4a2	XP_004912045.1
Homo sapiens (human)	COL4A2	NP_001837.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A dominant mutation in the EMB-9/type IV collagen alpha1 subunit can compensate for loss of FBL-1 activity in gonadogenesis.
Title: Tissue architecture in the Caenorhabditis elegans gonad depends on interactions among fibulin-1, type IV collagen and the ADAMTS extracellular protease.

The following emb-9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the emb-9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340084	NM_001027491.5 Latest version!	Caenorhabditis elegans Collagen alpha-1(IV) chain (emb-9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa340085	NM_001027492.5 Latest version!	Caenorhabditis elegans Collagen alpha-1(IV) chain (emb-9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OCa340085	NM_001027492.4	Caenorhabditis elegans Collagen alpha-1(IV) chain (emb-9), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCa340084	NM_001027491.4	Caenorhabditis elegans Collagen alpha-1(IV) chain (emb-9), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

emb-9 ( NM_001027491.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022662.2 Caenorhabditis elegans Collagen alpha-1(IV) chain (emb-9), mRNA.

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CloneID	OCa340084
Clone ID Related Accession (Same CDS sequence)	NM_001027491.4 , NM_001027491.5
Accession Version	NM_001027491.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Collagen alpha-1(IV) chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001027491.4.

ATGTCACGCT TATCGCTTCT CGGCTTGACG GCAGCCGTAG TGCTACTGTC GTCGTTCTGT 
CAAGATAGGA TACATGTGGA TGCTGCCGCC GCATGCAAGG GATGTGCTCC ACCATGTGTT 
TGCCCAGGAA CCAAAGGAGA ACGTGGTAAT CCAGGATTTG GTGGTGAACC AGGACATCCT 
GGAGCACCAG GACAAGATGG ACCAGAAGGA GCACCAGGAG CTCCAGGAAT GTTTGGAGCC 
GAAGGAGATT TTGGAGATAT GGGATCTAAG GGAGCTCGTG GAGATCGTGG TCTTCCTGGA 
TCGCCAGGTC ATCCAGGTCT TCAAGGACTT GACGGATTAC CAGGACTGAA AGGAGAAGAA 
GGAATTCCAG GATGCAATGG AACAGATGGT TTCCCTGGAA TGCCCGGACT TGCTGGACCT 
CCAGGGCAAT CTGGACAAAA CGGAAACCCT GGACGACCAG GACTCTCCGG ACCACCAGGA 
GAAGGAGGTG TCAATTCACA AGGACGCAAA GGAGTTAAAG GAGAATCTGG AAGATCAGGA 
GTTCCAGGTC TTCCAGGAAA CTCTGGTTAT CCTGGATTGA AAGGAGCAAA GGGAGATCCA 
GGACCGTACG GTCTTCCCGG ATTCCCCGGA GTTTCTGGAT TGAAGGGAAG AATGGGAGTG 
AGAACTTCTG GTGTTAAGGG AGAGAAGGGT TTGCCTGGAC CACCAGGACC ACCAGGACAA 
CCGGGATCTT ATCCATGGGC TTCAAAGCCA ATTGAGATGG AAGTCTTGCA AGGACCTGTC 
GGACCAGCTG GAGTGAAAGG AGAAAAGGGA CGTGATGGAC CAGTAGGACC ACCAGGAATG 
CTCGGACTTG ACGGACCACC AGGATATCCT GGATTAAAGG GACAGAAGGG AGATTTGGGA 
GATGCTGGAC AACGTGGTAA ACGTGGAAAG GACGGAGTTC CAGGAAATTA TGGAGAAAAG 
GGATCCCAAG GAGAACAAGG ACTTGGAGGA ACTCCAGGAT ACCCAGGAAC TAAGGGAGGG 
GCTGGAGAAC CAGGATACCC AGGAAGACCA GGTTTCGAAG GAGACTGTGG ACCGGAAGGA 
CCACTTGGAG AAGGAACTGG TGAGGCTGGA CCACATGGAG CTCAAGGATT CGACGGAGTT 
CAAGGAGGCA AAGGATTGCC AGGACATGAT GGTCTCCCAG GACCAGTAGG TCCAAGAGGT 
CCAGTTGGAG CTCCAGGAGC CCCAGGACAG CCCGGTATCG ATGGAATGCC AGGATACACC 
GAAAAAGGAG ATAGAGGAGA GGACGGTTAC CCAGGATTCG CTGGCGAACC AGGACTCCCA 
GGAGAACCAG GAGATTGTGG TTATCCAGGA GAGGATGGTC TTCCAGGATA CGATATTCAA 
GGACCACCTG GACTTGACGG ACAATCCGGA AGAGATGGAT TCCCTGGTAT TCCAGGAGAC 
ATCGGAGATC CAGGTTATTC TGGAGAAAAG GGATTCCCAG GAACTGGAGT TAACAAAGTT 
GGACCACCAG GAATGACCGG TTTGCCAGGA GAGCCAGGAA TGCCAGGACG TATTGGAGTT 
GACGGTTATC CAGGACCACC AGGAAACAAT GGAGAAAGAG GAGAAGACTG TGGATATTGT 
CCAGATGGTG TTCCAGGAAA TGCTGGAGAT CCAGGATTCC CCGGAATGAA TGGATATCCA 
GGACCACCAG GACCTAATGG TGATCATGGA GACTGCGGAA TGCCAGGAGC TCCAGGAAAG 
CCAGGATCCG CTGGATCAGA TGGACTTTCA GGATCACCAG GACTTCCAGG AATTCCAGGC 
TACCCAGGAA TGAAGGGAGA AGCCGGAGAG ATCGTTGGAC CAATGGAAAA CCCAGCTGGA 
ATTCCAGGTC TTAAGGGAGA TCATGGTCTT CCAGGACTAC CAGGACGACC AGGAAGTGAC 
GGACTCCCTG GTTACCCAGG AGGACCAGGA CAAAATGGAT TCCCAGGACT CCAAGGTGAA 
CCAGGTCTTG CTGGAATTGA TGGGAAGAGA GGACGTCAAG GATCTCTCGG AATCCCAGGA 
CTTCAAGGAC CACCTGGAGA CTCTTTCCCA GGACAGCCAG GAACACCTGG TTACAAGGGA 
GAACGAGGTG CTGATGGTCT TCCAGGACTT CCAGGAGCTC AAGGACCACG GGGAATTCCA 
GCACCATTGA GAATTGTCAA TCAAGTTGCT GGACAACCAG GTGTTGACGG CATGCCAGGT 
CTTCCAGGAG ATAGAGGAGC TGATGGTCTT CCAGGACTCC CAGGACCGGT TGGACCAGAC 
GGATACCCAG GAACACCAGG AGAGCGCGGT ATGGATGGTC TTCCAGGATT CCCAGGACTC 
CATGGAGAGC CTGGAATGCG TGGACAGCAA GGAGAAGTTG GTTTCAACGG AATTGATGGG 
GACTGTGGAG AGCCAGGTCT TGACGGATAC CCCGGAGCAC CAGGAGCTCC AGGAGCTCCA 
GGAGAGACTG GATTTGGCTT CCCAGGACAA GTCGGATACC CAGGACCAAA TGGAGATGCT 
GGGGCAGCTG GACTTCCAGG ACCAGACGGC TACCCAGGAA GAGACGGTCT TCCAGGAACT 
CCTGGATACC CAGGAGAAGC AGGAATGAAC GGACAAGATG GAGCCCCAGG ACAGCCAGGA 
TCTCGTGGAG AGTCTGGACT TGTTGGAATT GATGGAAAGA AAGGACGCGA TGGAACCCCA 
GGAACACGTG GACAAGACGG TGGACCGGGA TATTCCGGAG AGGCTGGAGC CCCAGGACAA 
AATGGAATGG ATGGATACCC AGGAGCACCA GGAGATCAAG GATATCCAGG ATCCCCAGGG 
CAAGACGGAT ACCCAGGGCC AAGTGGAATT CCAGGAGAGG ACGGTCTTGT CGGATTCCCA 
GGACTTCGTG GTGAACACGG AGACAATGGT CTTCCAGGAT TGGAGGGAGA ATGCGGAGAG 
GAAGGATCCA GAGGACTTGA TGGTGTTCCA GGTTACCCAG GAGAACACGG AACCGACGGG 
CTACCAGGGC TTCCAGGAGC TGATGGTCAA CCAGGTTTTG TTGGAGAAGC CGGAGAGCCA 
GGTACACCAG GATACAGAGG TCAGCCAGGA GAACCAGGAA ACCTCGCATA TCCAGGACAG 
CCAGGAGATG TTGGATACCC AGGACCAGAT GGACCACCAG GTCTTCCAGG ACAAGACGGC 
CTTCCAGGAC TGAATGGTGA ACGAGGAGAC AATGGAGATA GTTATCCAGG AAACCCAGGA 
TTGAGCGGCC AACCAGGAGA TGCTGGATAT GACGGACTTG ATGGAGTCCC AGGACCACCA 
GGATACCCAG GAATCACTGG GATGCCAGGG CTCAAGGGAG AATCTGGACT TCCAGGACTT 
CCAGGACGTC AAGGAAATGA CGGCATTCCA GGTCAACCAG GACTTGAAGG AGAATGTGGT 
GAAGATGGGT TCCCAGGATC TCCAGGGCAA CCAGGTTATC CTGGGCAGCA AGGACGTGAA 
GGAGAGAAGG GATATCCAGG AATTCCAGGA GAAAATGGTC TCCCTGGTCT TCGTGGACAA 
GACGGCCAGC CAGGTCTCAA GGGAGAAAAT GGTTTGGATG GTCAGCCAGG TTATCCAGGA 
TCCGCAGGAC AATTGGGAAC TCCAGGAGAC GTTGGATATC CAGGAGCACC TGGAGAAAAT 
GGAGACAACG GAAATCAGGG ACGTGATGGA CAACCTGGAC TTCGTGGAGA ATCAGGTCAG 
CCAGGACAGC CAGGATTGCC AGGAAGAGAT GGTCAGCCAG GACCAGTTGG ACCACCAGGA 
GACGATGGAT ACCCAGGAGC ACCCGGACAA GACATATATG GCCCACCAGG TCAAGCTGGA 
CAAGATGGCT ACCCAGGACT TGATGGACTC CCAGGAGCCC CAGGACTTAA TGGAGAACCA 
GGATCTCCAG GACAATACGG AATGCCAGGT CTTCCAGGAG GCCCAGGAGA ATCAGGACTA 
CCAGGATATC CAGGAGAACG TGGTCTTCCC GGACTCGACG GAAAGCGCGG TCATGATGGA 
CTCCCAGGAG CACCAGGTGT ACCAGGAGTT GAGGGAGTTC CAGGACTTGA AGGAGATTGT 
GGAGAAGACG GTTATCCAGG AGCTCCAGGA GCCCCAGGAA GCAACGGATA TCCAGGAGAG 
CGTGGTCTTC CAGGTGTCCC AGGACAACAA GGACGATCTG GTGACAACGG TTATCCAGGA 
GCACCAGGAC AACCTGGAAT CAAGGGACCA CGTGGAGATG ATGGATTCCC AGGACGCGAC 
GGACTCGACG GACTTCCAGG TCGACCAGGA CGTGAAGGAT TGCCCGGACC AATGGCTATG 
GCAGTTAGAA ACCCGCCAGG CCAACCAGGA GAAAATGGTT ATCCAGGAGA GAAGGGATAC 
CCAGGACTTC CAGGAGATAA CGGACTTTCC GGACCACCAG GAAAAGCCGG ATACCCAGGA 
GCTCCAGGAA CAGACGGCTA TCCAGGACCA CCAGGTCTTT CAGGAATGCC TGGACACGGT 
GGAGATCAAG GATTCCAAGG AGCTGCTGGA CGTACTGGTA ATCCAGGACT TCCAGGTACT 
CCAGGATACC CAGGATCACC AGGAGGATGG GCACCAAGTC GAGGATTCAC TTTTGCCAAG 
CACTCTCAGA CTACCGCAGT ACCACAGTGC CCACCAGGAG CTTCTCAACT TTGGGAAGGA 
TATTCTCTTC TTTACGTTCA AGGAAACGGT CGTGCCAGTG GACAAGATCT TGGTCAACCA 
GGATCTTGCC TCTCCAAATT CAACACAATG CCATTCATGT TCTGTAATAT GAACAGCGTC 
TGCCACGTTT CCAGCCGTAA CGATTACTCG TTCTGGTTGT CAACTGACGA GCCAATGACA 
CCAATGATGA ATCCAGTCAC CGGAACAGCA ATTCGTCCAT ACATTTCTCG TTGTGCCGTC 
TGTGAAGTTC CAACTCAAAT CATCGCAGTT CACTCTCAAG ACACATCAGT TCCACAATGT 
CCACAAGGAT GGTCGGGTAT GTGGACCGGA TATTCCTTTG TCATGCACAC TGCCGCTGGA 
GCCGAAGGAA CTGGACAAAG TCTTCAATCG CCTGGTTCCT GTCTCGAAGA GTTCCGTGCC 
GTTCCATTCA TTGAGTGCCA CGGAAGAGGA ACATGCAATT ATTATGCCAC CAATCACGGA 
TTCTGGCTTT CCATCGTTGA TCAGGACAAG CAATTCCGTA AACCAATGTC ACAGACACTC 
AAAGCTGGAG GACTTAAAGA TCGTGTATCC AGATGCCAAG TGTGTCTCAA GAACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022662.2
CDS	50..5329
Translation	MSRLSLLGLTAAVVLLSSFCQDRIHVDAAAACKGCAPPCVCPGTKGERGNPGFGGEPGHPGAPGQDGPEGAPGAPGMFGAEGDFGDMGSKGARGDRGLPGSPGHPGLQGLDGLPGLKGEEGIPGCNGTDGFPGMPGLAGPPGQSGQNGNPGRPGLSGPPGEGGVNSQGRKGVKGESGRSGVPGLPGNSGYPGLKGAKGDPGPYGLPGFPGVSGLKGRMGVRTSGVKGEKGLPGPPGPPGQPGSYPWASKPIEMEVLQGPVGPAGVKGEKGRDGPVGPPGMLGLDGPPGYPGLKGQKGDLGDAGQRGKRGKDGVPGNYGEKGSQGEQGLGGTPGYPGTKGGAGEPGYPGRPGFEGDCGPEGPLGEGTGEAGPHGAQGFDGVQGGKGLPGHDGLPGPVGPRGPVGAPGAPGQPGIDGMPGYTEKGDRGEDGYPGFAGEPGLPGEPGDCGYPGEDGLPGYDIQGPPGLDGQSGRDGFPGIPGDIGDPGYSGEKGFPGTGVNKVGPPGMTGLPGEPGMPGRIGVDGYPGPPGNNGERGEDCGYCPDGVPGNAGDPGFPGMNGYPGPPGPNGDHGDCGMPGAPGKPGSAGSDGLSGSPGLPGIPGYPGMKGEAGEIVGPMENPAGIPGLKGDHGLPGLPGRPGSDGLPGYPGGPGQNGFPGLQGEPGLAGIDGKRGRQGSLGIPGLQGPPGDSFPGQPGTPGYKGERGADGLPGLPGAQGPRGIPAPLRIVNQVAGQPGVDGMPGLPGDRGADGLPGLPGPVGPDGYPGTPGERGMDGLPGFPGLHGEPGMRGQQGEVGFNGIDGDCGEPGLDGYPGAPGAPGAPGETGFGFPGQVGYPGPNGDAGAAGLPGPDGYPGRDGLPGTPGYPGEAGMNGQDGAPGQPGSRGESGLVGIDGKKGRDGTPGTRGQDGGPGYSGEAGAPGQNGMDGYPGAPGDQGYPGSPGQDGYPGPSGIPGEDGLVGFPGLRGEHGDNGLPGLEGECGEEGSRGLDGVPGYPGEHGTDGLPGLPGADGQPGFVGEAGEPGTPGYRGQPGEPGNLAYPGQPGDVGYPGPDGPPGLPGQDGLPGLNGERGDNGDSYPGNPGLSGQPGDAGYDGLDGVPGPPGYPGITGMPGLKGESGLPGLPGRQGNDGIPGQPGLEGECGEDGFPGSPGQPGYPGQQGREGEKGYPGIPGENGLPGLRGQDGQPGLKGENGLDGQPGYPGSAGQLGTPGDVGYPGAPGENGDNGNQGRDGQPGLRGESGQPGQPGLPGRDGQPGPVGPPGDDGYPGAPGQDIYGPPGQAGQDGYPGLDGLPGAPGLNGEPGSPGQYGMPGLPGGPGESGLPGYPGERGLPGLDGKRGHDGLPGAPGVPGVEGVPGLEGDCGEDGYPGAPGAPGSNGYPGERGLPGVPGQQGRSGDNGYPGAPGQPGIKGPRGDDGFPGRDGLDGLPGRPGREGLPGPMAMAVRNPPGQPGENGYPGEKGYPGLPGDNGLSGPPGKAGYPGAPGTDGYPGPPGLSGMPGHGGDQGFQGAAGRTGNPGLPGTPGYPGSPGGWAPSRGFTFAKHSQTTAVPQCPPGASQLWEGYSLLYVQGNGRASGQDLGQPGSCLSKFNTMPFMFCNMNSVCHVSSRNDYSFWLSTDEPMTPMMNPVTGTAIRPYISRCAVCEVPTQIIAVHSQDTSVPQCPQGWSGMWTGYSFVMHTAAGAEGTGQSLQSPGSCLEEFRAVPFIECHGRGTCNYYATNHGFWLSIVDQDKQFRKPMSQTLKAGGLKDRVSRCQVCLKNR

Target ORF information:

RefSeq Version	NM_001027491.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Collagen alpha-1(IV) chain (emb-9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027491.5

ORF Insert Sequence:

ATGTCACGCT TATCGCTTCT CGGCTTGACG GCAGCCGTAG TGCTACTGTC GTCGTTCTGT 
CAAGATAGGA TACATGTGGA TGCTGCCGCC GCATGCAAGG GATGTGCTCC ACCATGTGTT 
TGCCCAGGAA CCAAAGGAGA ACGTGGTAAT CCAGGATTTG GTGGTGAACC AGGACATCCT 
GGAGCACCAG GACAAGATGG ACCAGAAGGA GCACCAGGAG CTCCAGGAAT GTTTGGAGCC 
GAAGGAGATT TTGGAGATAT GGGATCTAAG GGAGCTCGTG GAGATCGTGG TCTTCCTGGA 
TCGCCAGGTC ATCCAGGTCT TCAAGGACTT GACGGATTAC CAGGACTGAA AGGAGAAGAA 
GGAATTCCAG GATGCAATGG AACAGATGGT TTCCCTGGAA TGCCCGGACT TGCTGGACCT 
CCAGGGCAAT CTGGACAAAA CGGAAACCCT GGACGACCAG GACTCTCCGG ACCACCAGGA 
GAAGGAGGTG TCAATTCACA AGGACGCAAA GGAGTTAAAG GAGAATCTGG AAGATCAGGA 
GTTCCAGGTC TTCCAGGAAA CTCTGGTTAT CCTGGATTGA AAGGAGCAAA GGGAGATCCA 
GGACCGTACG GTCTTCCCGG ATTCCCCGGA GTTTCTGGAT TGAAGGGAAG AATGGGAGTG 
AGAACTTCTG GTGTTAAGGG AGAGAAGGGT TTGCCTGGAC CACCAGGACC ACCAGGACAA 
CCGGGATCTT ATCCATGGGC TTCAAAGCCA ATTGAGATGG AAGTCTTGCA AGGACCTGTC 
GGACCAGCTG GAGTGAAAGG AGAAAAGGGA CGTGATGGAC CAGTAGGACC ACCAGGAATG 
CTCGGACTTG ACGGACCACC AGGATATCCT GGATTAAAGG GACAGAAGGG AGATTTGGGA 
GATGCTGGAC AACGTGGTAA ACGTGGAAAG GACGGAGTTC CAGGAAATTA TGGAGAAAAG 
GGATCCCAAG GAGAACAAGG ACTTGGAGGA ACTCCAGGAT ACCCAGGAAC TAAGGGAGGG 
GCTGGAGAAC CAGGATACCC AGGAAGACCA GGTTTCGAAG GAGACTGTGG ACCGGAAGGA 
CCACTTGGAG AAGGAACTGG TGAGGCTGGA CCACATGGAG CTCAAGGATT CGACGGAGTT 
CAAGGAGGCA AAGGATTGCC AGGACATGAT GGTCTCCCAG GACCAGTAGG TCCAAGAGGT 
CCAGTTGGAG CTCCAGGAGC CCCAGGACAG CCCGGTATCG ATGGAATGCC AGGATACACC 
GAAAAAGGAG ATAGAGGAGA GGACGGTTAC CCAGGATTCG CTGGCGAACC AGGACTCCCA 
GGAGAACCAG GAGATTGTGG TTATCCAGGA GAGGATGGTC TTCCAGGATA CGATATTCAA 
GGACCACCTG GACTTGACGG ACAATCCGGA AGAGATGGAT TCCCTGGTAT TCCAGGAGAC 
ATCGGAGATC CAGGTTATTC TGGAGAAAAG GGATTCCCAG GAACTGGAGT TAACAAAGTT 
GGACCACCAG GAATGACCGG TTTGCCAGGA GAGCCAGGAA TGCCAGGACG TATTGGAGTT 
GACGGTTATC CAGGACCACC AGGAAACAAT GGAGAAAGAG GAGAAGACTG TGGATATTGT 
CCAGATGGTG TTCCAGGAAA TGCTGGAGAT CCAGGATTCC CCGGAATGAA TGGATATCCA 
GGACCACCAG GACCTAATGG TGATCATGGA GACTGCGGAA TGCCAGGAGC TCCAGGAAAG 
CCAGGATCCG CTGGATCAGA TGGACTTTCA GGATCACCAG GACTTCCAGG AATTCCAGGC 
TACCCAGGAA TGAAGGGAGA AGCCGGAGAG ATCGTTGGAC CAATGGAAAA CCCAGCTGGA 
ATTCCAGGTC TTAAGGGAGA TCATGGTCTT CCAGGACTAC CAGGACGACC AGGAAGTGAC 
GGACTCCCTG GTTACCCAGG AGGACCAGGA CAAAATGGAT TCCCAGGACT CCAAGGTGAA 
CCAGGTCTTG CTGGAATTGA TGGGAAGAGA GGACGTCAAG GATCTCTCGG AATCCCAGGA 
CTTCAAGGAC CACCTGGAGA CTCTTTCCCA GGACAGCCAG GAACACCTGG TTACAAGGGA 
GAACGAGGTG CTGATGGTCT TCCAGGACTT CCAGGAGCTC AAGGACCACG GGGAATTCCA 
GCACCATTGA GAATTGTCAA TCAAGTTGCT GGACAACCAG GTGTTGACGG CATGCCAGGT 
CTTCCAGGAG ATAGAGGAGC TGATGGTCTT CCAGGACTCC CAGGACCGGT TGGACCAGAC 
GGATACCCAG GAACACCAGG AGAGCGCGGT ATGGATGGTC TTCCAGGATT CCCAGGACTC 
CATGGAGAGC CTGGAATGCG TGGACAGCAA GGAGAAGTTG GTTTCAACGG AATTGATGGG 
GACTGTGGAG AGCCAGGTCT TGACGGATAC CCCGGAGCAC CAGGAGCTCC AGGAGCTCCA 
GGAGAGACTG GATTTGGCTT CCCAGGACAA GTCGGATACC CAGGACCAAA TGGAGATGCT 
GGGGCAGCTG GACTTCCAGG ACCAGACGGC TACCCAGGAA GAGACGGTCT TCCAGGAACT 
CCTGGATACC CAGGAGAAGC AGGAATGAAC GGACAAGATG GAGCCCCAGG ACAGCCAGGA 
TCTCGTGGAG AGTCTGGACT TGTTGGAATT GATGGAAAGA AAGGACGCGA TGGAACCCCA 
GGAACACGTG GACAAGACGG TGGACCGGGA TATTCCGGAG AGGCTGGAGC CCCAGGACAA 
AATGGAATGG ATGGATACCC AGGAGCACCA GGAGATCAAG GATATCCAGG ATCCCCAGGG 
CAAGACGGAT ACCCAGGGCC AAGTGGAATT CCAGGAGAGG ACGGTCTTGT CGGATTCCCA 
GGACTTCGTG GTGAACACGG AGACAATGGT CTTCCAGGAT TGGAGGGAGA ATGCGGAGAG 
GAAGGATCCA GAGGACTTGA TGGTGTTCCA GGTTACCCAG GAGAACACGG AACCGACGGG 
CTACCAGGGC TTCCAGGAGC TGATGGTCAA CCAGGTTTTG TTGGAGAAGC CGGAGAGCCA 
GGTACACCAG GATACAGAGG TCAGCCAGGA GAACCAGGAA ACCTCGCATA TCCAGGACAG 
CCAGGAGATG TTGGATACCC AGGACCAGAT GGACCACCAG GTCTTCCAGG ACAAGACGGC 
CTTCCAGGAC TGAATGGTGA ACGAGGAGAC AATGGAGATA GTTATCCAGG AAACCCAGGA 
TTGAGCGGCC AACCAGGAGA TGCTGGATAT GACGGACTTG ATGGAGTCCC AGGACCACCA 
GGATACCCAG GAATCACTGG GATGCCAGGG CTCAAGGGAG AATCTGGACT TCCAGGACTT 
CCAGGACGTC AAGGAAATGA CGGCATTCCA GGTCAACCAG GACTTGAAGG AGAATGTGGT 
GAAGATGGGT TCCCAGGATC TCCAGGGCAA CCAGGTTATC CTGGGCAGCA AGGACGTGAA 
GGAGAGAAGG GATATCCAGG AATTCCAGGA GAAAATGGTC TCCCTGGTCT TCGTGGACAA 
GACGGCCAGC CAGGTCTCAA GGGAGAAAAT GGTTTGGATG GTCAGCCAGG TTATCCAGGA 
TCCGCAGGAC AATTGGGAAC TCCAGGAGAC GTTGGATATC CAGGAGCACC TGGAGAAAAT 
GGAGACAACG GAAATCAGGG ACGTGATGGA CAACCTGGAC TTCGTGGAGA ATCAGGTCAG 
CCAGGACAGC CAGGATTGCC AGGAAGAGAT GGTCAGCCAG GACCAGTTGG ACCACCAGGA 
GACGATGGAT ACCCAGGAGC ACCCGGACAA GACATATATG GCCCACCAGG TCAAGCTGGA 
CAAGATGGCT ACCCAGGACT TGATGGACTC CCAGGAGCCC CAGGACTTAA TGGAGAACCA 
GGATCTCCAG GACAATACGG AATGCCAGGT CTTCCAGGAG GCCCAGGAGA ATCAGGACTA 
CCAGGATATC CAGGAGAACG TGGTCTTCCC GGACTCGACG GAAAGCGCGG TCATGATGGA 
CTCCCAGGAG CACCAGGTGT ACCAGGAGTT GAGGGAGTTC CAGGACTTGA AGGAGATTGT 
GGAGAAGACG GTTATCCAGG AGCTCCAGGA GCCCCAGGAA GCAACGGATA TCCAGGAGAG 
CGTGGTCTTC CAGGTGTCCC AGGACAACAA GGACGATCTG GTGACAACGG TTATCCAGGA 
GCACCAGGAC AACCTGGAAT CAAGGGACCA CGTGGAGATG ATGGATTCCC AGGACGCGAC 
GGACTCGACG GACTTCCAGG TCGACCAGGA CGTGAAGGAT TGCCCGGACC AATGGCTATG 
GCAGTTAGAA ACCCGCCAGG CCAACCAGGA GAAAATGGTT ATCCAGGAGA GAAGGGATAC 
CCAGGACTTC CAGGAGATAA CGGACTTTCC GGACCACCAG GAAAAGCCGG ATACCCAGGA 
GCTCCAGGAA CAGACGGCTA TCCAGGACCA CCAGGTCTTT CAGGAATGCC TGGACACGGT 
GGAGATCAAG GATTCCAAGG AGCTGCTGGA CGTACTGGTA ATCCAGGACT TCCAGGTACT 
CCAGGATACC CAGGATCACC AGGAGGATGG GCACCAAGTC GAGGATTCAC TTTTGCCAAG 
CACTCTCAGA CTACCGCAGT ACCACAGTGC CCACCAGGAG CTTCTCAACT TTGGGAAGGA 
TATTCTCTTC TTTACGTTCA AGGAAACGGT CGTGCCAGTG GACAAGATCT TGGTCAACCA 
GGATCTTGCC TCTCCAAATT CAACACAATG CCATTCATGT TCTGTAATAT GAACAGCGTC 
TGCCACGTTT CCAGCCGTAA CGATTACTCG TTCTGGTTGT CAACTGACGA GCCAATGACA 
CCAATGATGA ATCCAGTCAC CGGAACAGCA ATTCGTCCAT ACATTTCTCG TTGTGCCGTC 
TGTGAAGTTC CAACTCAAAT CATCGCAGTT CACTCTCAAG ACACATCAGT TCCACAATGT 
CCACAAGGAT GGTCGGGTAT GTGGACCGGA TATTCCTTTG TCATGCACAC TGCCGCTGGA 
GCCGAAGGAA CTGGACAAAG TCTTCAATCG CCTGGTTCCT GTCTCGAAGA GTTCCGTGCC 
GTTCCATTCA TTGAGTGCCA CGGAAGAGGA ACATGCAATT ATTATGCCAC CAATCACGGA 
TTCTGGCTTT CCATCGTTGA TCAGGACAAG CAATTCCGTA AACCAATGTC ACAGACACTC 
AAAGCTGGAG GACTTAAAGA TCGTGTATCC AGATGCCAAG TGTGTCTCAA GAACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emb-9 ( NM_001027492.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022663.1 Caenorhabditis elegans Collagen alpha-1(IV) chain (emb-9), mRNA.

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CloneID	OCa340085
Clone ID Related Accession (Same CDS sequence)	NM_001027492.4 , NM_001027492.5
Accession Version	NM_001027492.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Collagen alpha-1(IV) chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001027492.4.

ATGTCACGCT TATCGCTTCT CGGCTTGACG GCAGCCGTAG TGCTACTGTC GTCGTTCTGT 
CAAGATAGGA TACATGTGGA TGCTGCCGCC GCATGCAAGG GATGTGCTCC ACCATGTGTT 
TGCCCAGGAA CCAAAGGAGA ACGTGGTAAT CCAGGATTTG GTGGTGAACC AGGACATCCT 
GGAGCACCAG GACAAGATGG ACCAGAAGGA GCACCAGGAG CTCCAGGAAT GTTTGGAGCC 
GAAGGAGATT TTGGAGATAT GGGATCTAAG GGAGCTCGTG GAGATCGTGG TCTTCCTGGA 
TCGCCAGGTC ATCCAGGTCT TCAAGGACTT GACGGATTAC CAGGACTGAA AGGAGAAGAA 
GGAATTCCAG GATGCAATGG AACAGATGGT TTCCCTGGAA TGCCCGGACT TGCTGGACCT 
CCAGGGCAAT CTGGACAAAA CGGAAACCCT GGACGACCAG GACTCTCCGG ACCACCAGGA 
GAAGGAGGTG TCAATTCACA AGGACGCAAA GGAGTTAAAG GAGAATCTGG AAGATCAGGA 
GTTCCAGGTC TTCCAGGAAA CTCTGGTTAT CCTGGATTGA AAGGAGCAAA GGGAGATCCA 
GGACCGTACG GTCTTCCCGG ATTCCCCGGA GTTTCTGGAT TGAAGGGAAG AATGGGAGTG 
AGAACTTCTG GTGTTAAGGG AGAGAAGGGT TTGCCTGGAC CACCAGGACC ACCAGGACAA 
CCGGGATCTT ATCCATGGGC TTCAAAGCCA ATTGAGATGG AAGTCTTGCA AGGACCTGTC 
GGACCAGCTG GAGTGAAAGG AGAAAAGGGA CGTGATGGAC CAGTAGGACC ACCAGGAATG 
CTCGGACTTG ACGGACCACC AGGATATCCT GGATTAAAGG GACAGAAGGG AGATTTGGGA 
GATGCTGGAC AACGTGGTAA ACGTGGAAAG GACGGAGTTC CAGGAAATTA TGGAGAAAAG 
GGATCCCAAG GAGAACAAGG ACTTGGAGGA ACTCCAGGAT ACCCAGGAAC TAAGGGAGGG 
GCTGGAGAAC CAGGATACCC AGGAAGACCA GGTTTCGAAG GAGACTGTGG ACCGGAAGGA 
CCACTTGGAG AAGGAACTGG TGAGGCTGGA CCACATGGAG CTCAAGGATT CGACGGAGTT 
CAAGGAGGCA AAGGATTGCC AGGACATGAT GGTCTCCCAG GACCAGTAGG TCCAAGAGGT 
CCAGTTGGAG CTCCAGGAGC CCCAGGACAG CCCGGTATCG ATGGAATGCC AGGATACACC 
GAAAAAGGAG ATAGAGGAGA GGACGGTTAC CCAGGATTCG CTGGCGAACC AGGACTCCCA 
GGAGAACCAG GAGATTGTGG TTATCCAGGA GAGGATGGTC TTCCAGGATA CGATATTCAA 
GGACCACCTG GACTTGACGG ACAATCCGGA AGAGATGGAT TCCCTGGTAT TCCAGGAGAC 
ATCGGAGATC CAGGTTATTC TGGAGAAAAG GGATTCCCAG GAACTGGAGT TAACAAAGTT 
GGACCAGACG GATACCCAGG AACACCAGGA GAGCGCGGTA TGGATGGTCT TCCAGGATTC 
CCAGGACTCC ATGGAGAGCC TGGAATGCGT GGACAGCAAG GAGAAGTTGG TTTCAACGGA 
ATTGATGGGG ACTGTGGAGA GCCAGGTCTT GACGGATACC CCGGAGCACC AGGAGCTCCA 
GGAGCTCCAG GAGAGACTGG ATTTGGCTTC CCAGGACAAG TCGGATACCC AGGACCAAAT 
GGAGATGCTG GGGCAGCTGG ACTTCCAGGA CCAGACGGCT ACCCAGGAAG AGACGGTCTT 
CCAGGAACTC CTGGATACCC AGGAGAAGCA GGAATGAACG GACAAGATGG AGCCCCAGGA 
CAGCCAGGAT CTCGTGGAGA GTCTGGACTT GTTGGAATTG ATGGAAAGAA AGGACGCGAT 
GGAACCCCAG GAACACGTGG ACAAGACGGT GGACCGGGAT ATTCCGGAGA GGCTGGAGCC 
CCAGGACAAA ATGGAATGGA TGGATACCCA GGAGCACCAG GAGATCAAGG ATATCCAGGA 
TCCCCAGGGC AAGACGGATA CCCAGGGCCA AGTGGAATTC CAGGAGAGGA CGGTCTTGTC 
GGATTCCCAG GACTTCGTGG TGAACACGGA GACAATGGTC TTCCAGGATT GGAGGGAGAA 
TGCGGAGAGG AAGGATCCAG AGGACTTGAT GGTGTTCCAG GTTACCCAGG AGAACACGGA 
ACCGACGGGC TACCAGGGCT TCCAGGAGCT GATGGTCAAC CAGGTTTTGT TGGAGAAGCC 
GGAGAGCCAG GTACACCAGG ATACAGAGGT CAGCCAGGAG AACCAGGAAA CCTCGCATAT 
CCAGGACAGC CAGGAGATGT TGGATACCCA GGACCAGATG GACCACCAGG TCTTCCAGGA 
CAAGACGGCC TTCCAGGACT GAATGGTGAA CGAGGAGACA ATGGAGATAG TTATCCAGGA 
AACCCAGGAT TGAGCGGCCA ACCAGGAGAT GCTGGATATG ACGGACTTGA TGGAGTCCCA 
GGACCACCAG GATACCCAGG AATCACTGGG ATGCCAGGGC TCAAGGGAGA ATCTGGACTT 
CCAGGACTTC CAGGACGTCA AGGAAATGAC GGCATTCCAG GTCAACCAGG ACTTGAAGGA 
GAATGTGGTG AAGATGGGTT CCCAGGATCT CCAGGGCAAC CAGGTTATCC TGGGCAGCAA 
GGACGTGAAG GAGAGAAGGG ATATCCAGGA ATTCCAGGAG AAAATGGTCT CCCTGGTCTT 
CGTGGACAAG ACGGCCAGCC AGGTCTCAAG GGAGAAAATG GTTTGGATGG TCAGCCAGGT 
TATCCAGGAT CCGCAGGACA ATTGGGAACT CCAGGAGACG TTGGATATCC AGGAGCACCT 
GGAGAAAATG GAGACAACGG AAATCAGGGA CGTGATGGAC AACCTGGACT TCGTGGAGAA 
TCAGGTCAGC CAGGACAGCC AGGATTGCCA GGAAGAGATG GTCAGCCAGG ACCAGTTGGA 
CCACCAGGAG ACGATGGATA CCCAGGAGCA CCCGGACAAG ACATATATGG CCCACCAGGT 
CAAGCTGGAC AAGATGGCTA CCCAGGACTT GATGGACTCC CAGGAGCCCC AGGACTTAAT 
GGAGAACCAG GATCTCCAGG ACAATACGGA ATGCCAGGTC TTCCAGGAGG CCCAGGAGAA 
TCAGGACTAC CAGGATATCC AGGAGAACGT GGTCTTCCCG GACTCGACGG AAAGCGCGGT 
CATGATGGAC TCCCAGGAGC ACCAGGTGTA CCAGGAGTTG AGGGAGTTCC AGGACTTGAA 
GGAGATTGTG GAGAAGACGG TTATCCAGGA GCTCCAGGAG CCCCAGGAAG CAACGGATAT 
CCAGGAGAGC GTGGTCTTCC AGGTGTCCCA GGACAACAAG GACGATCTGG TGACAACGGT 
TATCCAGGAG CACCAGGACA ACCTGGAATC AAGGGACCAC GTGGAGATGA TGGATTCCCA 
GGACGCGACG GACTCGACGG ACTTCCAGGT CGACCAGGAC GTGAAGGATT GCCCGGACCA 
ATGGCTATGG CAGTTAGAAA CCCGCCAGGC CAACCAGGAG AAAATGGTTA TCCAGGAGAG 
AAGGGATACC CAGGACTTCC AGGAGATAAC GGACTTTCCG GACCACCAGG AAAAGCCGGA 
TACCCAGGAG CTCCAGGAAC AGACGGCTAT CCAGGACCAC CAGGTCTTTC AGGAATGCCT 
GGACACGGTG GAGATCAAGG ATTCCAAGGA GCTGCTGGAC GTACTGGTAA TCCAGGACTT 
CCAGGTACTC CAGGATACCC AGGATCACCA GGAGGATGGG CACCAAGTCG AGGATTCACT 
TTTGCCAAGC ACTCTCAGAC TACCGCAGTA CCACAGTGCC CACCAGGAGC TTCTCAACTT 
TGGGAAGGAT ATTCTCTTCT TTACGTTCAA GGAAACGGTC GTGCCAGTGG ACAAGATCTT 
GGTCAACCAG GATCTTGCCT CTCCAAATTC AACACAATGC CATTCATGTT CTGTAATATG 
AACAGCGTCT GCCACGTTTC CAGCCGTAAC GATTACTCGT TCTGGTTGTC AACTGACGAG 
CCAATGACAC CAATGATGAA TCCAGTCACC GGAACAGCAA TTCGTCCATA CATTTCTCGT 
TGTGCCGTCT GTGAAGTTCC AACTCAAATC ATCGCAGTTC ACTCTCAAGA CACATCAGTT 
CCACAATGTC CACAAGGATG GTCGGGTATG TGGACCGGAT ATTCCTTTGT CATGCACACT 
GCCGCTGGAG CCGAAGGAAC TGGACAAAGT CTTCAATCGC CTGGTTCCTG TCTCGAAGAG 
TTCCGTGCCG TTCCATTCAT TGAGTGCCAC GGAAGAGGAA CATGCAATTA TTATGCCACC 
AATCACGGAT TCTGGCTTTC CATCGTTGAT CAGGACAAGC AATTCCGTAA ACCAATGTCA 
CAGACACTCA AAGCTGGAGG ACTTAAAGAT CGTGTATCCA GATGCCAAGT GTGTCTCAAG 
AACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022663.1
CDS	50..4558
Translation	MSRLSLLGLTAAVVLLSSFCQDRIHVDAAAACKGCAPPCVCPGTKGERGNPGFGGEPGHPGAPGQDGPEGAPGAPGMFGAEGDFGDMGSKGARGDRGLPGSPGHPGLQGLDGLPGLKGEEGIPGCNGTDGFPGMPGLAGPPGQSGQNGNPGRPGLSGPPGEGGVNSQGRKGVKGESGRSGVPGLPGNSGYPGLKGAKGDPGPYGLPGFPGVSGLKGRMGVRTSGVKGEKGLPGPPGPPGQPGSYPWASKPIEMEVLQGPVGPAGVKGEKGRDGPVGPPGMLGLDGPPGYPGLKGQKGDLGDAGQRGKRGKDGVPGNYGEKGSQGEQGLGGTPGYPGTKGGAGEPGYPGRPGFEGDCGPEGPLGEGTGEAGPHGAQGFDGVQGGKGLPGHDGLPGPVGPRGPVGAPGAPGQPGIDGMPGYTEKGDRGEDGYPGFAGEPGLPGEPGDCGYPGEDGLPGYDIQGPPGLDGQSGRDGFPGIPGDIGDPGYSGEKGFPGTGVNKVGPDGYPGTPGERGMDGLPGFPGLHGEPGMRGQQGEVGFNGIDGDCGEPGLDGYPGAPGAPGAPGETGFGFPGQVGYPGPNGDAGAAGLPGPDGYPGRDGLPGTPGYPGEAGMNGQDGAPGQPGSRGESGLVGIDGKKGRDGTPGTRGQDGGPGYSGEAGAPGQNGMDGYPGAPGDQGYPGSPGQDGYPGPSGIPGEDGLVGFPGLRGEHGDNGLPGLEGECGEEGSRGLDGVPGYPGEHGTDGLPGLPGADGQPGFVGEAGEPGTPGYRGQPGEPGNLAYPGQPGDVGYPGPDGPPGLPGQDGLPGLNGERGDNGDSYPGNPGLSGQPGDAGYDGLDGVPGPPGYPGITGMPGLKGESGLPGLPGRQGNDGIPGQPGLEGECGEDGFPGSPGQPGYPGQQGREGEKGYPGIPGENGLPGLRGQDGQPGLKGENGLDGQPGYPGSAGQLGTPGDVGYPGAPGENGDNGNQGRDGQPGLRGESGQPGQPGLPGRDGQPGPVGPPGDDGYPGAPGQDIYGPPGQAGQDGYPGLDGLPGAPGLNGEPGSPGQYGMPGLPGGPGESGLPGYPGERGLPGLDGKRGHDGLPGAPGVPGVEGVPGLEGDCGEDGYPGAPGAPGSNGYPGERGLPGVPGQQGRSGDNGYPGAPGQPGIKGPRGDDGFPGRDGLDGLPGRPGREGLPGPMAMAVRNPPGQPGENGYPGEKGYPGLPGDNGLSGPPGKAGYPGAPGTDGYPGPPGLSGMPGHGGDQGFQGAAGRTGNPGLPGTPGYPGSPGGWAPSRGFTFAKHSQTTAVPQCPPGASQLWEGYSLLYVQGNGRASGQDLGQPGSCLSKFNTMPFMFCNMNSVCHVSSRNDYSFWLSTDEPMTPMMNPVTGTAIRPYISRCAVCEVPTQIIAVHSQDTSVPQCPQGWSGMWTGYSFVMHTAAGAEGTGQSLQSPGSCLEEFRAVPFIECHGRGTCNYYATNHGFWLSIVDQDKQFRKPMSQTLKAGGLKDRVSRCQVCLKNR

Target ORF information:

RefSeq Version	NM_001027492.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Collagen alpha-1(IV) chain (emb-9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027492.5

ORF Insert Sequence:

ATGTCACGCT TATCGCTTCT CGGCTTGACG GCAGCCGTAG TGCTACTGTC GTCGTTCTGT 
CAAGATAGGA TACATGTGGA TGCTGCCGCC GCATGCAAGG GATGTGCTCC ACCATGTGTT 
TGCCCAGGAA CCAAAGGAGA ACGTGGTAAT CCAGGATTTG GTGGTGAACC AGGACATCCT 
GGAGCACCAG GACAAGATGG ACCAGAAGGA GCACCAGGAG CTCCAGGAAT GTTTGGAGCC 
GAAGGAGATT TTGGAGATAT GGGATCTAAG GGAGCTCGTG GAGATCGTGG TCTTCCTGGA 
TCGCCAGGTC ATCCAGGTCT TCAAGGACTT GACGGATTAC CAGGACTGAA AGGAGAAGAA 
GGAATTCCAG GATGCAATGG AACAGATGGT TTCCCTGGAA TGCCCGGACT TGCTGGACCT 
CCAGGGCAAT CTGGACAAAA CGGAAACCCT GGACGACCAG GACTCTCCGG ACCACCAGGA 
GAAGGAGGTG TCAATTCACA AGGACGCAAA GGAGTTAAAG GAGAATCTGG AAGATCAGGA 
GTTCCAGGTC TTCCAGGAAA CTCTGGTTAT CCTGGATTGA AAGGAGCAAA GGGAGATCCA 
GGACCGTACG GTCTTCCCGG ATTCCCCGGA GTTTCTGGAT TGAAGGGAAG AATGGGAGTG 
AGAACTTCTG GTGTTAAGGG AGAGAAGGGT TTGCCTGGAC CACCAGGACC ACCAGGACAA 
CCGGGATCTT ATCCATGGGC TTCAAAGCCA ATTGAGATGG AAGTCTTGCA AGGACCTGTC 
GGACCAGCTG GAGTGAAAGG AGAAAAGGGA CGTGATGGAC CAGTAGGACC ACCAGGAATG 
CTCGGACTTG ACGGACCACC AGGATATCCT GGATTAAAGG GACAGAAGGG AGATTTGGGA 
GATGCTGGAC AACGTGGTAA ACGTGGAAAG GACGGAGTTC CAGGAAATTA TGGAGAAAAG 
GGATCCCAAG GAGAACAAGG ACTTGGAGGA ACTCCAGGAT ACCCAGGAAC TAAGGGAGGG 
GCTGGAGAAC CAGGATACCC AGGAAGACCA GGTTTCGAAG GAGACTGTGG ACCGGAAGGA 
CCACTTGGAG AAGGAACTGG TGAGGCTGGA CCACATGGAG CTCAAGGATT CGACGGAGTT 
CAAGGAGGCA AAGGATTGCC AGGACATGAT GGTCTCCCAG GACCAGTAGG TCCAAGAGGT 
CCAGTTGGAG CTCCAGGAGC CCCAGGACAG CCCGGTATCG ATGGAATGCC AGGATACACC 
GAAAAAGGAG ATAGAGGAGA GGACGGTTAC CCAGGATTCG CTGGCGAACC AGGACTCCCA 
GGAGAACCAG GAGATTGTGG TTATCCAGGA GAGGATGGTC TTCCAGGATA CGATATTCAA 
GGACCACCTG GACTTGACGG ACAATCCGGA AGAGATGGAT TCCCTGGTAT TCCAGGAGAC 
ATCGGAGATC CAGGTTATTC TGGAGAAAAG GGATTCCCAG GAACTGGAGT TAACAAAGTT 
GGACCAGACG GATACCCAGG AACACCAGGA GAGCGCGGTA TGGATGGTCT TCCAGGATTC 
CCAGGACTCC ATGGAGAGCC TGGAATGCGT GGACAGCAAG GAGAAGTTGG TTTCAACGGA 
ATTGATGGGG ACTGTGGAGA GCCAGGTCTT GACGGATACC CCGGAGCACC AGGAGCTCCA 
GGAGCTCCAG GAGAGACTGG ATTTGGCTTC CCAGGACAAG TCGGATACCC AGGACCAAAT 
GGAGATGCTG GGGCAGCTGG ACTTCCAGGA CCAGACGGCT ACCCAGGAAG AGACGGTCTT 
CCAGGAACTC CTGGATACCC AGGAGAAGCA GGAATGAACG GACAAGATGG AGCCCCAGGA 
CAGCCAGGAT CTCGTGGAGA GTCTGGACTT GTTGGAATTG ATGGAAAGAA AGGACGCGAT 
GGAACCCCAG GAACACGTGG ACAAGACGGT GGACCGGGAT ATTCCGGAGA GGCTGGAGCC 
CCAGGACAAA ATGGAATGGA TGGATACCCA GGAGCACCAG GAGATCAAGG ATATCCAGGA 
TCCCCAGGGC AAGACGGATA CCCAGGGCCA AGTGGAATTC CAGGAGAGGA CGGTCTTGTC 
GGATTCCCAG GACTTCGTGG TGAACACGGA GACAATGGTC TTCCAGGATT GGAGGGAGAA 
TGCGGAGAGG AAGGATCCAG AGGACTTGAT GGTGTTCCAG GTTACCCAGG AGAACACGGA 
ACCGACGGGC TACCAGGGCT TCCAGGAGCT GATGGTCAAC CAGGTTTTGT TGGAGAAGCC 
GGAGAGCCAG GTACACCAGG ATACAGAGGT CAGCCAGGAG AACCAGGAAA CCTCGCATAT 
CCAGGACAGC CAGGAGATGT TGGATACCCA GGACCAGATG GACCACCAGG TCTTCCAGGA 
CAAGACGGCC TTCCAGGACT GAATGGTGAA CGAGGAGACA ATGGAGATAG TTATCCAGGA 
AACCCAGGAT TGAGCGGCCA ACCAGGAGAT GCTGGATATG ACGGACTTGA TGGAGTCCCA 
GGACCACCAG GATACCCAGG AATCACTGGG ATGCCAGGGC TCAAGGGAGA ATCTGGACTT 
CCAGGACTTC CAGGACGTCA AGGAAATGAC GGCATTCCAG GTCAACCAGG ACTTGAAGGA 
GAATGTGGTG AAGATGGGTT CCCAGGATCT CCAGGGCAAC CAGGTTATCC TGGGCAGCAA 
GGACGTGAAG GAGAGAAGGG ATATCCAGGA ATTCCAGGAG AAAATGGTCT CCCTGGTCTT 
CGTGGACAAG ACGGCCAGCC AGGTCTCAAG GGAGAAAATG GTTTGGATGG TCAGCCAGGT 
TATCCAGGAT CCGCAGGACA ATTGGGAACT CCAGGAGACG TTGGATATCC AGGAGCACCT 
GGAGAAAATG GAGACAACGG AAATCAGGGA CGTGATGGAC AACCTGGACT TCGTGGAGAA 
TCAGGTCAGC CAGGACAGCC AGGATTGCCA GGAAGAGATG GTCAGCCAGG ACCAGTTGGA 
CCACCAGGAG ACGATGGATA CCCAGGAGCA CCCGGACAAG ACATATATGG CCCACCAGGT 
CAAGCTGGAC AAGATGGCTA CCCAGGACTT GATGGACTCC CAGGAGCCCC AGGACTTAAT 
GGAGAACCAG GATCTCCAGG ACAATACGGA ATGCCAGGTC TTCCAGGAGG CCCAGGAGAA 
TCAGGACTAC CAGGATATCC AGGAGAACGT GGTCTTCCCG GACTCGACGG AAAGCGCGGT 
CATGATGGAC TCCCAGGAGC ACCAGGTGTA CCAGGAGTTG AGGGAGTTCC AGGACTTGAA 
GGAGATTGTG GAGAAGACGG TTATCCAGGA GCTCCAGGAG CCCCAGGAAG CAACGGATAT 
CCAGGAGAGC GTGGTCTTCC AGGTGTCCCA GGACAACAAG GACGATCTGG TGACAACGGT 
TATCCAGGAG CACCAGGACA ACCTGGAATC AAGGGACCAC GTGGAGATGA TGGATTCCCA 
GGACGCGACG GACTCGACGG ACTTCCAGGT CGACCAGGAC GTGAAGGATT GCCCGGACCA 
ATGGCTATGG CAGTTAGAAA CCCGCCAGGC CAACCAGGAG AAAATGGTTA TCCAGGAGAG 
AAGGGATACC CAGGACTTCC AGGAGATAAC GGACTTTCCG GACCACCAGG AAAAGCCGGA 
TACCCAGGAG CTCCAGGAAC AGACGGCTAT CCAGGACCAC CAGGTCTTTC AGGAATGCCT 
GGACACGGTG GAGATCAAGG ATTCCAAGGA GCTGCTGGAC GTACTGGTAA TCCAGGACTT 
CCAGGTACTC CAGGATACCC AGGATCACCA GGAGGATGGG CACCAAGTCG AGGATTCACT 
TTTGCCAAGC ACTCTCAGAC TACCGCAGTA CCACAGTGCC CACCAGGAGC TTCTCAACTT 
TGGGAAGGAT ATTCTCTTCT TTACGTTCAA GGAAACGGTC GTGCCAGTGG ACAAGATCTT 
GGTCAACCAG GATCTTGCCT CTCCAAATTC AACACAATGC CATTCATGTT CTGTAATATG 
AACAGCGTCT GCCACGTTTC CAGCCGTAAC GATTACTCGT TCTGGTTGTC AACTGACGAG 
CCAATGACAC CAATGATGAA TCCAGTCACC GGAACAGCAA TTCGTCCATA CATTTCTCGT 
TGTGCCGTCT GTGAAGTTCC AACTCAAATC ATCGCAGTTC ACTCTCAAGA CACATCAGTT 
CCACAATGTC CACAAGGATG GTCGGGTATG TGGACCGGAT ATTCCTTTGT CATGCACACT 
GCCGCTGGAG CCGAAGGAAC TGGACAAAGT CTTCAATCGC CTGGTTCCTG TCTCGAAGAG 
TTCCGTGCCG TTCCATTCAT TGAGTGCCAC GGAAGAGGAA CATGCAATTA TTATGCCACC 
AATCACGGAT TCTGGCTTTC CATCGTTGAT CAGGACAAGC AATTCCGTAA ACCAATGTCA 
CAGACACTCA AAGCTGGAGG ACTTAAAGAT CGTGTATCCA GATGCCAAGT GTGTCTCAAG 
AACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emb-9 ( NM_001027491.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022662.2 Caenorhabditis elegans Collagen alpha-1(IV) chain (emb-9), partial mRNA.

Price & Availability↑

CloneID	OCa340084
Clone ID Related Accession (Same CDS sequence)	NM_001027491.4 , NM_001027491.5
Accession Version	NM_001027491.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Collagen alpha-1(IV) chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Feb 27, 2013 this sequence version replaced NM_001027491.3. COMPLETENESS: incomplete on both ends.

ATGTCACGCT TATCGCTTCT CGGCTTGACG GCAGCCGTAG TGCTACTGTC GTCGTTCTGT 
CAAGATAGGA TACATGTGGA TGCTGCCGCC GCATGCAAGG GATGTGCTCC ACCATGTGTT 
TGCCCAGGAA CCAAAGGAGA ACGTGGTAAT CCAGGATTTG GTGGTGAACC AGGACATCCT 
GGAGCACCAG GACAAGATGG ACCAGAAGGA GCACCAGGAG CTCCAGGAAT GTTTGGAGCC 
GAAGGAGATT TTGGAGATAT GGGATCTAAG GGAGCTCGTG GAGATCGTGG TCTTCCTGGA 
TCGCCAGGTC ATCCAGGTCT TCAAGGACTT GACGGATTAC CAGGACTGAA AGGAGAAGAA 
GGAATTCCAG GATGCAATGG AACAGATGGT TTCCCTGGAA TGCCCGGACT TGCTGGACCT 
CCAGGGCAAT CTGGACAAAA CGGAAACCCT GGACGACCAG GACTCTCCGG ACCACCAGGA 
GAAGGAGGTG TCAATTCACA AGGACGCAAA GGAGTTAAAG GAGAATCTGG AAGATCAGGA 
GTTCCAGGTC TTCCAGGAAA CTCTGGTTAT CCTGGATTGA AAGGAGCAAA GGGAGATCCA 
GGACCGTACG GTCTTCCCGG ATTCCCCGGA GTTTCTGGAT TGAAGGGAAG AATGGGAGTG 
AGAACTTCTG GTGTTAAGGG AGAGAAGGGT TTGCCTGGAC CACCAGGACC ACCAGGACAA 
CCGGGATCTT ATCCATGGGC TTCAAAGCCA ATTGAGATGG AAGTCTTGCA AGGACCTGTC 
GGACCAGCTG GAGTGAAAGG AGAAAAGGGA CGTGATGGAC CAGTAGGACC ACCAGGAATG 
CTCGGACTTG ACGGACCACC AGGATATCCT GGATTAAAGG GACAGAAGGG AGATTTGGGA 
GATGCTGGAC AACGTGGTAA ACGTGGAAAG GACGGAGTTC CAGGAAATTA TGGAGAAAAG 
GGATCCCAAG GAGAACAAGG ACTTGGAGGA ACTCCAGGAT ACCCAGGAAC TAAGGGAGGG 
GCTGGAGAAC CAGGATACCC AGGAAGACCA GGTTTCGAAG GAGACTGTGG ACCGGAAGGA 
CCACTTGGAG AAGGAACTGG TGAGGCTGGA CCACATGGAG CTCAAGGATT CGACGGAGTT 
CAAGGAGGCA AAGGATTGCC AGGACATGAT GGTCTCCCAG GACCAGTAGG TCCAAGAGGT 
CCAGTTGGAG CTCCAGGAGC CCCAGGACAG CCCGGTATCG ATGGAATGCC AGGATACACC 
GAAAAAGGAG ATAGAGGAGA GGACGGTTAC CCAGGATTCG CTGGCGAACC AGGACTCCCA 
GGAGAACCAG GAGATTGTGG TTATCCAGGA GAGGATGGTC TTCCAGGATA CGATATTCAA 
GGACCACCTG GACTTGACGG ACAATCCGGA AGAGATGGAT TCCCTGGTAT TCCAGGAGAC 
ATCGGAGATC CAGGTTATTC TGGAGAAAAG GGATTCCCAG GAACTGGAGT TAACAAAGTT 
GGACCACCAG GAATGACCGG TTTGCCAGGA GAGCCAGGAA TGCCAGGACG TATTGGAGTT 
GACGGTTATC CAGGACCACC AGGAAACAAT GGAGAAAGAG GAGAAGACTG TGGATATTGT 
CCAGATGGTG TTCCAGGAAA TGCTGGAGAT CCAGGATTCC CCGGAATGAA TGGATATCCA 
GGACCACCAG GACCTAATGG TGATCATGGA GACTGCGGAA TGCCAGGAGC TCCAGGAAAG 
CCAGGATCCG CTGGATCAGA TGGACTTTCA GGATCACCAG GACTTCCAGG AATTCCAGGC 
TACCCAGGAA TGAAGGGAGA AGCCGGAGAG ATCGTTGGAC CAATGGAAAA CCCAGCTGGA 
ATTCCAGGTC TTAAGGGAGA TCATGGTCTT CCAGGACTAC CAGGACGACC AGGAAGTGAC 
GGACTCCCTG GTTACCCAGG AGGACCAGGA CAAAATGGAT TCCCAGGACT CCAAGGTGAA 
CCAGGTCTTG CTGGAATTGA TGGGAAGAGA GGACGTCAAG GATCTCTCGG AATCCCAGGA 
CTTCAAGGAC CACCTGGAGA CTCTTTCCCA GGACAGCCAG GAACACCTGG TTACAAGGGA 
GAACGAGGTG CTGATGGTCT TCCAGGACTT CCAGGAGCTC AAGGACCACG GGGAATTCCA 
GCACCATTGA GAATTGTCAA TCAAGTTGCT GGACAACCAG GTGTTGACGG CATGCCAGGT 
CTTCCAGGAG ATAGAGGAGC TGATGGTCTT CCAGGACTCC CAGGACCGGT TGGACCAGAC 
GGATACCCAG GAACACCAGG AGAGCGCGGT ATGGATGGTC TTCCAGGATT CCCAGGACTC 
CATGGAGAGC CTGGAATGCG TGGACAGCAA GGAGAAGTTG GTTTCAACGG AATTGATGGG 
GACTGTGGAG AGCCAGGTCT TGACGGATAC CCCGGAGCAC CAGGAGCTCC AGGAGCTCCA 
GGAGAGACTG GATTTGGCTT CCCAGGACAA GTCGGATACC CAGGACCAAA TGGAGATGCT 
GGGGCAGCTG GACTTCCAGG ACCAGACGGC TACCCAGGAA GAGACGGTCT TCCAGGAACT 
CCTGGATACC CAGGAGAAGC AGGAATGAAC GGACAAGATG GAGCCCCAGG ACAGCCAGGA 
TCTCGTGGAG AGTCTGGACT TGTTGGAATT GATGGAAAGA AAGGACGCGA TGGAACCCCA 
GGAACACGTG GACAAGACGG TGGACCGGGA TATTCCGGAG AGGCTGGAGC CCCAGGACAA 
AATGGAATGG ATGGATACCC AGGAGCACCA GGAGATCAAG GATATCCAGG ATCCCCAGGG 
CAAGACGGAT ACCCAGGGCC AAGTGGAATT CCAGGAGAGG ACGGTCTTGT CGGATTCCCA 
GGACTTCGTG GTGAACACGG AGACAATGGT CTTCCAGGAT TGGAGGGAGA ATGCGGAGAG 
GAAGGATCCA GAGGACTTGA TGGTGTTCCA GGTTACCCAG GAGAACACGG AACCGACGGG 
CTACCAGGGC TTCCAGGAGC TGATGGTCAA CCAGGTTTTG TTGGAGAAGC CGGAGAGCCA 
GGTACACCAG GATACAGAGG TCAGCCAGGA GAACCAGGAA ACCTCGCATA TCCAGGACAG 
CCAGGAGATG TTGGATACCC AGGACCAGAT GGACCACCAG GTCTTCCAGG ACAAGACGGC 
CTTCCAGGAC TGAATGGTGA ACGAGGAGAC AATGGAGATA GTTATCCAGG AAACCCAGGA 
TTGAGCGGCC AACCAGGAGA TGCTGGATAT GACGGACTTG ATGGAGTCCC AGGACCACCA 
GGATACCCAG GAATCACTGG GATGCCAGGG CTCAAGGGAG AATCTGGACT TCCAGGACTT 
CCAGGACGTC AAGGAAATGA CGGCATTCCA GGTCAACCAG GACTTGAAGG AGAATGTGGT 
GAAGATGGGT TCCCAGGATC TCCAGGGCAA CCAGGTTATC CTGGGCAGCA AGGACGTGAA 
GGAGAGAAGG GATATCCAGG AATTCCAGGA GAAAATGGTC TCCCTGGTCT TCGTGGACAA 
GACGGCCAGC CAGGTCTCAA GGGAGAAAAT GGTTTGGATG GTCAGCCAGG TTATCCAGGA 
TCCGCAGGAC AATTGGGAAC TCCAGGAGAC GTTGGATATC CAGGAGCACC TGGAGAAAAT 
GGAGACAACG GAAATCAGGG ACGTGATGGA CAACCTGGAC TTCGTGGAGA ATCAGGTCAG 
CCAGGACAGC CAGGATTGCC AGGAAGAGAT GGTCAGCCAG GACCAGTTGG ACCACCAGGA 
GACGATGGAT ACCCAGGAGC ACCCGGACAA GACATATATG GCCCACCAGG TCAAGCTGGA 
CAAGATGGCT ACCCAGGACT TGATGGACTC CCAGGAGCCC CAGGACTTAA TGGAGAACCA 
GGATCTCCAG GACAATACGG AATGCCAGGT CTTCCAGGAG GCCCAGGAGA ATCAGGACTA 
CCAGGATATC CAGGAGAACG TGGTCTTCCC GGACTCGACG GAAAGCGCGG TCATGATGGA 
CTCCCAGGAG CACCAGGTGT ACCAGGAGTT GAGGGAGTTC CAGGACTTGA AGGAGATTGT 
GGAGAAGACG GTTATCCAGG AGCTCCAGGA GCCCCAGGAA GCAACGGATA TCCAGGAGAG 
CGTGGTCTTC CAGGTGTCCC AGGACAACAA GGACGATCTG GTGACAACGG TTATCCAGGA 
GCACCAGGAC AACCTGGAAT CAAGGGACCA CGTGGAGATG ATGGATTCCC AGGACGCGAC 
GGACTCGACG GACTTCCAGG TCGACCAGGA CGTGAAGGAT TGCCCGGACC AATGGCTATG 
GCAGTTAGAA ACCCGCCAGG CCAACCAGGA GAAAATGGTT ATCCAGGAGA GAAGGGATAC 
CCAGGACTTC CAGGAGATAA CGGACTTTCC GGACCACCAG GAAAAGCCGG ATACCCAGGA 
GCTCCAGGAA CAGACGGCTA TCCAGGACCA CCAGGTCTTT CAGGAATGCC TGGACACGGT 
GGAGATCAAG GATTCCAAGG AGCTGCTGGA CGTACTGGTA ATCCAGGACT TCCAGGTACT 
CCAGGATACC CAGGATCACC AGGAGGATGG GCACCAAGTC GAGGATTCAC TTTTGCCAAG 
CACTCTCAGA CTACCGCAGT ACCACAGTGC CCACCAGGAG CTTCTCAACT TTGGGAAGGA 
TATTCTCTTC TTTACGTTCA AGGAAACGGT CGTGCCAGTG GACAAGATCT TGGTCAACCA 
GGATCTTGCC TCTCCAAATT CAACACAATG CCATTCATGT TCTGTAATAT GAACAGCGTC 
TGCCACGTTT CCAGCCGTAA CGATTACTCG TTCTGGTTGT CAACTGACGA GCCAATGACA 
CCAATGATGA ATCCAGTCAC CGGAACAGCA ATTCGTCCAT ACATTTCTCG TTGTGCCGTC 
TGTGAAGTTC CAACTCAAAT CATCGCAGTT CACTCTCAAG ACACATCAGT TCCACAATGT 
CCACAAGGAT GGTCGGGTAT GTGGACCGGA TATTCCTTTG TCATGCACAC TGCCGCTGGA 
GCCGAAGGAA CTGGACAAAG TCTTCAATCG CCTGGTTCCT GTCTCGAAGA GTTCCGTGCC 
GTTCCATTCA TTGAGTGCCA CGGAAGAGGA ACATGCAATT ATTATGCCAC CAATCACGGA 
TTCTGGCTTT CCATCGTTGA TCAGGACAAG CAATTCCGTA AACCAATGTC ACAGACACTC 
AAAGCTGGAG GACTTAAAGA TCGTGTATCC AGATGCCAAG TGTGTCTCAA GAACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022662.2
CDS	1..5280
Translation	MSRLSLLGLTAAVVLLSSFCQDRIHVDAAAACKGCAPPCVCPGTKGERGNPGFGGEPGHPGAPGQDGPEGAPGAPGMFGAEGDFGDMGSKGARGDRGLPGSPGHPGLQGLDGLPGLKGEEGIPGCNGTDGFPGMPGLAGPPGQSGQNGNPGRPGLSGPPGEGGVNSQGRKGVKGESGRSGVPGLPGNSGYPGLKGAKGDPGPYGLPGFPGVSGLKGRMGVRTSGVKGEKGLPGPPGPPGQPGSYPWASKPIEMEVLQGPVGPAGVKGEKGRDGPVGPPGMLGLDGPPGYPGLKGQKGDLGDAGQRGKRGKDGVPGNYGEKGSQGEQGLGGTPGYPGTKGGAGEPGYPGRPGFEGDCGPEGPLGEGTGEAGPHGAQGFDGVQGGKGLPGHDGLPGPVGPRGPVGAPGAPGQPGIDGMPGYTEKGDRGEDGYPGFAGEPGLPGEPGDCGYPGEDGLPGYDIQGPPGLDGQSGRDGFPGIPGDIGDPGYSGEKGFPGTGVNKVGPPGMTGLPGEPGMPGRIGVDGYPGPPGNNGERGEDCGYCPDGVPGNAGDPGFPGMNGYPGPPGPNGDHGDCGMPGAPGKPGSAGSDGLSGSPGLPGIPGYPGMKGEAGEIVGPMENPAGIPGLKGDHGLPGLPGRPGSDGLPGYPGGPGQNGFPGLQGEPGLAGIDGKRGRQGSLGIPGLQGPPGDSFPGQPGTPGYKGERGADGLPGLPGAQGPRGIPAPLRIVNQVAGQPGVDGMPGLPGDRGADGLPGLPGPVGPDGYPGTPGERGMDGLPGFPGLHGEPGMRGQQGEVGFNGIDGDCGEPGLDGYPGAPGAPGAPGETGFGFPGQVGYPGPNGDAGAAGLPGPDGYPGRDGLPGTPGYPGEAGMNGQDGAPGQPGSRGESGLVGIDGKKGRDGTPGTRGQDGGPGYSGEAGAPGQNGMDGYPGAPGDQGYPGSPGQDGYPGPSGIPGEDGLVGFPGLRGEHGDNGLPGLEGECGEEGSRGLDGVPGYPGEHGTDGLPGLPGADGQPGFVGEAGEPGTPGYRGQPGEPGNLAYPGQPGDVGYPGPDGPPGLPGQDGLPGLNGERGDNGDSYPGNPGLSGQPGDAGYDGLDGVPGPPGYPGITGMPGLKGESGLPGLPGRQGNDGIPGQPGLEGECGEDGFPGSPGQPGYPGQQGREGEKGYPGIPGENGLPGLRGQDGQPGLKGENGLDGQPGYPGSAGQLGTPGDVGYPGAPGENGDNGNQGRDGQPGLRGESGQPGQPGLPGRDGQPGPVGPPGDDGYPGAPGQDIYGPPGQAGQDGYPGLDGLPGAPGLNGEPGSPGQYGMPGLPGGPGESGLPGYPGERGLPGLDGKRGHDGLPGAPGVPGVEGVPGLEGDCGEDGYPGAPGAPGSNGYPGERGLPGVPGQQGRSGDNGYPGAPGQPGIKGPRGDDGFPGRDGLDGLPGRPGREGLPGPMAMAVRNPPGQPGENGYPGEKGYPGLPGDNGLSGPPGKAGYPGAPGTDGYPGPPGLSGMPGHGGDQGFQGAAGRTGNPGLPGTPGYPGSPGGWAPSRGFTFAKHSQTTAVPQCPPGASQLWEGYSLLYVQGNGRASGQDLGQPGSCLSKFNTMPFMFCNMNSVCHVSSRNDYSFWLSTDEPMTPMMNPVTGTAIRPYISRCAVCEVPTQIIAVHSQDTSVPQCPQGWSGMWTGYSFVMHTAAGAEGTGQSLQSPGSCLEEFRAVPFIECHGRGTCNYYATNHGFWLSIVDQDKQFRKPMSQTLKAGGLKDRVSRCQVCLKNR

Target ORF information:

RefSeq Version	NM_001027491.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Collagen alpha-1(IV) chain (emb-9), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027491.4

ORF Insert Sequence:

ATGTCACGCT TATCGCTTCT CGGCTTGACG GCAGCCGTAG TGCTACTGTC GTCGTTCTGT 
CAAGATAGGA TACATGTGGA TGCTGCCGCC GCATGCAAGG GATGTGCTCC ACCATGTGTT 
TGCCCAGGAA CCAAAGGAGA ACGTGGTAAT CCAGGATTTG GTGGTGAACC AGGACATCCT 
GGAGCACCAG GACAAGATGG ACCAGAAGGA GCACCAGGAG CTCCAGGAAT GTTTGGAGCC 
GAAGGAGATT TTGGAGATAT GGGATCTAAG GGAGCTCGTG GAGATCGTGG TCTTCCTGGA 
TCGCCAGGTC ATCCAGGTCT TCAAGGACTT GACGGATTAC CAGGACTGAA AGGAGAAGAA 
GGAATTCCAG GATGCAATGG AACAGATGGT TTCCCTGGAA TGCCCGGACT TGCTGGACCT 
CCAGGGCAAT CTGGACAAAA CGGAAACCCT GGACGACCAG GACTCTCCGG ACCACCAGGA 
GAAGGAGGTG TCAATTCACA AGGACGCAAA GGAGTTAAAG GAGAATCTGG AAGATCAGGA 
GTTCCAGGTC TTCCAGGAAA CTCTGGTTAT CCTGGATTGA AAGGAGCAAA GGGAGATCCA 
GGACCGTACG GTCTTCCCGG ATTCCCCGGA GTTTCTGGAT TGAAGGGAAG AATGGGAGTG 
AGAACTTCTG GTGTTAAGGG AGAGAAGGGT TTGCCTGGAC CACCAGGACC ACCAGGACAA 
CCGGGATCTT ATCCATGGGC TTCAAAGCCA ATTGAGATGG AAGTCTTGCA AGGACCTGTC 
GGACCAGCTG GAGTGAAAGG AGAAAAGGGA CGTGATGGAC CAGTAGGACC ACCAGGAATG 
CTCGGACTTG ACGGACCACC AGGATATCCT GGATTAAAGG GACAGAAGGG AGATTTGGGA 
GATGCTGGAC AACGTGGTAA ACGTGGAAAG GACGGAGTTC CAGGAAATTA TGGAGAAAAG 
GGATCCCAAG GAGAACAAGG ACTTGGAGGA ACTCCAGGAT ACCCAGGAAC TAAGGGAGGG 
GCTGGAGAAC CAGGATACCC AGGAAGACCA GGTTTCGAAG GAGACTGTGG ACCGGAAGGA 
CCACTTGGAG AAGGAACTGG TGAGGCTGGA CCACATGGAG CTCAAGGATT CGACGGAGTT 
CAAGGAGGCA AAGGATTGCC AGGACATGAT GGTCTCCCAG GACCAGTAGG TCCAAGAGGT 
CCAGTTGGAG CTCCAGGAGC CCCAGGACAG CCCGGTATCG ATGGAATGCC AGGATACACC 
GAAAAAGGAG ATAGAGGAGA GGACGGTTAC CCAGGATTCG CTGGCGAACC AGGACTCCCA 
GGAGAACCAG GAGATTGTGG TTATCCAGGA GAGGATGGTC TTCCAGGATA CGATATTCAA 
GGACCACCTG GACTTGACGG ACAATCCGGA AGAGATGGAT TCCCTGGTAT TCCAGGAGAC 
ATCGGAGATC CAGGTTATTC TGGAGAAAAG GGATTCCCAG GAACTGGAGT TAACAAAGTT 
GGACCACCAG GAATGACCGG TTTGCCAGGA GAGCCAGGAA TGCCAGGACG TATTGGAGTT 
GACGGTTATC CAGGACCACC AGGAAACAAT GGAGAAAGAG GAGAAGACTG TGGATATTGT 
CCAGATGGTG TTCCAGGAAA TGCTGGAGAT CCAGGATTCC CCGGAATGAA TGGATATCCA 
GGACCACCAG GACCTAATGG TGATCATGGA GACTGCGGAA TGCCAGGAGC TCCAGGAAAG 
CCAGGATCCG CTGGATCAGA TGGACTTTCA GGATCACCAG GACTTCCAGG AATTCCAGGC 
TACCCAGGAA TGAAGGGAGA AGCCGGAGAG ATCGTTGGAC CAATGGAAAA CCCAGCTGGA 
ATTCCAGGTC TTAAGGGAGA TCATGGTCTT CCAGGACTAC CAGGACGACC AGGAAGTGAC 
GGACTCCCTG GTTACCCAGG AGGACCAGGA CAAAATGGAT TCCCAGGACT CCAAGGTGAA 
CCAGGTCTTG CTGGAATTGA TGGGAAGAGA GGACGTCAAG GATCTCTCGG AATCCCAGGA 
CTTCAAGGAC CACCTGGAGA CTCTTTCCCA GGACAGCCAG GAACACCTGG TTACAAGGGA 
GAACGAGGTG CTGATGGTCT TCCAGGACTT CCAGGAGCTC AAGGACCACG GGGAATTCCA 
GCACCATTGA GAATTGTCAA TCAAGTTGCT GGACAACCAG GTGTTGACGG CATGCCAGGT 
CTTCCAGGAG ATAGAGGAGC TGATGGTCTT CCAGGACTCC CAGGACCGGT TGGACCAGAC 
GGATACCCAG GAACACCAGG AGAGCGCGGT ATGGATGGTC TTCCAGGATT CCCAGGACTC 
CATGGAGAGC CTGGAATGCG TGGACAGCAA GGAGAAGTTG GTTTCAACGG AATTGATGGG 
GACTGTGGAG AGCCAGGTCT TGACGGATAC CCCGGAGCAC CAGGAGCTCC AGGAGCTCCA 
GGAGAGACTG GATTTGGCTT CCCAGGACAA GTCGGATACC CAGGACCAAA TGGAGATGCT 
GGGGCAGCTG GACTTCCAGG ACCAGACGGC TACCCAGGAA GAGACGGTCT TCCAGGAACT 
CCTGGATACC CAGGAGAAGC AGGAATGAAC GGACAAGATG GAGCCCCAGG ACAGCCAGGA 
TCTCGTGGAG AGTCTGGACT TGTTGGAATT GATGGAAAGA AAGGACGCGA TGGAACCCCA 
GGAACACGTG GACAAGACGG TGGACCGGGA TATTCCGGAG AGGCTGGAGC CCCAGGACAA 
AATGGAATGG ATGGATACCC AGGAGCACCA GGAGATCAAG GATATCCAGG ATCCCCAGGG 
CAAGACGGAT ACCCAGGGCC AAGTGGAATT CCAGGAGAGG ACGGTCTTGT CGGATTCCCA 
GGACTTCGTG GTGAACACGG AGACAATGGT CTTCCAGGAT TGGAGGGAGA ATGCGGAGAG 
GAAGGATCCA GAGGACTTGA TGGTGTTCCA GGTTACCCAG GAGAACACGG AACCGACGGG 
CTACCAGGGC TTCCAGGAGC TGATGGTCAA CCAGGTTTTG TTGGAGAAGC CGGAGAGCCA 
GGTACACCAG GATACAGAGG TCAGCCAGGA GAACCAGGAA ACCTCGCATA TCCAGGACAG 
CCAGGAGATG TTGGATACCC AGGACCAGAT GGACCACCAG GTCTTCCAGG ACAAGACGGC 
CTTCCAGGAC TGAATGGTGA ACGAGGAGAC AATGGAGATA GTTATCCAGG AAACCCAGGA 
TTGAGCGGCC AACCAGGAGA TGCTGGATAT GACGGACTTG ATGGAGTCCC AGGACCACCA 
GGATACCCAG GAATCACTGG GATGCCAGGG CTCAAGGGAG AATCTGGACT TCCAGGACTT 
CCAGGACGTC AAGGAAATGA CGGCATTCCA GGTCAACCAG GACTTGAAGG AGAATGTGGT 
GAAGATGGGT TCCCAGGATC TCCAGGGCAA CCAGGTTATC CTGGGCAGCA AGGACGTGAA 
GGAGAGAAGG GATATCCAGG AATTCCAGGA GAAAATGGTC TCCCTGGTCT TCGTGGACAA 
GACGGCCAGC CAGGTCTCAA GGGAGAAAAT GGTTTGGATG GTCAGCCAGG TTATCCAGGA 
TCCGCAGGAC AATTGGGAAC TCCAGGAGAC GTTGGATATC CAGGAGCACC TGGAGAAAAT 
GGAGACAACG GAAATCAGGG ACGTGATGGA CAACCTGGAC TTCGTGGAGA ATCAGGTCAG 
CCAGGACAGC CAGGATTGCC AGGAAGAGAT GGTCAGCCAG GACCAGTTGG ACCACCAGGA 
GACGATGGAT ACCCAGGAGC ACCCGGACAA GACATATATG GCCCACCAGG TCAAGCTGGA 
CAAGATGGCT ACCCAGGACT TGATGGACTC CCAGGAGCCC CAGGACTTAA TGGAGAACCA 
GGATCTCCAG GACAATACGG AATGCCAGGT CTTCCAGGAG GCCCAGGAGA ATCAGGACTA 
CCAGGATATC CAGGAGAACG TGGTCTTCCC GGACTCGACG GAAAGCGCGG TCATGATGGA 
CTCCCAGGAG CACCAGGTGT ACCAGGAGTT GAGGGAGTTC CAGGACTTGA AGGAGATTGT 
GGAGAAGACG GTTATCCAGG AGCTCCAGGA GCCCCAGGAA GCAACGGATA TCCAGGAGAG 
CGTGGTCTTC CAGGTGTCCC AGGACAACAA GGACGATCTG GTGACAACGG TTATCCAGGA 
GCACCAGGAC AACCTGGAAT CAAGGGACCA CGTGGAGATG ATGGATTCCC AGGACGCGAC 
GGACTCGACG GACTTCCAGG TCGACCAGGA CGTGAAGGAT TGCCCGGACC AATGGCTATG 
GCAGTTAGAA ACCCGCCAGG CCAACCAGGA GAAAATGGTT ATCCAGGAGA GAAGGGATAC 
CCAGGACTTC CAGGAGATAA CGGACTTTCC GGACCACCAG GAAAAGCCGG ATACCCAGGA 
GCTCCAGGAA CAGACGGCTA TCCAGGACCA CCAGGTCTTT CAGGAATGCC TGGACACGGT 
GGAGATCAAG GATTCCAAGG AGCTGCTGGA CGTACTGGTA ATCCAGGACT TCCAGGTACT 
CCAGGATACC CAGGATCACC AGGAGGATGG GCACCAAGTC GAGGATTCAC TTTTGCCAAG 
CACTCTCAGA CTACCGCAGT ACCACAGTGC CCACCAGGAG CTTCTCAACT TTGGGAAGGA 
TATTCTCTTC TTTACGTTCA AGGAAACGGT CGTGCCAGTG GACAAGATCT TGGTCAACCA 
GGATCTTGCC TCTCCAAATT CAACACAATG CCATTCATGT TCTGTAATAT GAACAGCGTC 
TGCCACGTTT CCAGCCGTAA CGATTACTCG TTCTGGTTGT CAACTGACGA GCCAATGACA 
CCAATGATGA ATCCAGTCAC CGGAACAGCA ATTCGTCCAT ACATTTCTCG TTGTGCCGTC 
TGTGAAGTTC CAACTCAAAT CATCGCAGTT CACTCTCAAG ACACATCAGT TCCACAATGT 
CCACAAGGAT GGTCGGGTAT GTGGACCGGA TATTCCTTTG TCATGCACAC TGCCGCTGGA 
GCCGAAGGAA CTGGACAAAG TCTTCAATCG CCTGGTTCCT GTCTCGAAGA GTTCCGTGCC 
GTTCCATTCA TTGAGTGCCA CGGAAGAGGA ACATGCAATT ATTATGCCAC CAATCACGGA 
TTCTGGCTTT CCATCGTTGA TCAGGACAAG CAATTCCGTA AACCAATGTC ACAGACACTC 
AAAGCTGGAG GACTTAAAGA TCGTGTATCC AGATGCCAAG TGTGTCTCAA GAACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emb-9 ( NM_001027492.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022663.1 Caenorhabditis elegans Collagen alpha-1(IV) chain (emb-9), partial mRNA.

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CloneID	OCa340085
Clone ID Related Accession (Same CDS sequence)	NM_001027492.4 , NM_001027492.5
Accession Version	NM_001027492.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Collagen alpha-1(IV) chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_001027492.3. COMPLETENESS: incomplete on both ends.

ATGTCACGCT TATCGCTTCT CGGCTTGACG GCAGCCGTAG TGCTACTGTC GTCGTTCTGT 
CAAGATAGGA TACATGTGGA TGCTGCCGCC GCATGCAAGG GATGTGCTCC ACCATGTGTT 
TGCCCAGGAA CCAAAGGAGA ACGTGGTAAT CCAGGATTTG GTGGTGAACC AGGACATCCT 
GGAGCACCAG GACAAGATGG ACCAGAAGGA GCACCAGGAG CTCCAGGAAT GTTTGGAGCC 
GAAGGAGATT TTGGAGATAT GGGATCTAAG GGAGCTCGTG GAGATCGTGG TCTTCCTGGA 
TCGCCAGGTC ATCCAGGTCT TCAAGGACTT GACGGATTAC CAGGACTGAA AGGAGAAGAA 
GGAATTCCAG GATGCAATGG AACAGATGGT TTCCCTGGAA TGCCCGGACT TGCTGGACCT 
CCAGGGCAAT CTGGACAAAA CGGAAACCCT GGACGACCAG GACTCTCCGG ACCACCAGGA 
GAAGGAGGTG TCAATTCACA AGGACGCAAA GGAGTTAAAG GAGAATCTGG AAGATCAGGA 
GTTCCAGGTC TTCCAGGAAA CTCTGGTTAT CCTGGATTGA AAGGAGCAAA GGGAGATCCA 
GGACCGTACG GTCTTCCCGG ATTCCCCGGA GTTTCTGGAT TGAAGGGAAG AATGGGAGTG 
AGAACTTCTG GTGTTAAGGG AGAGAAGGGT TTGCCTGGAC CACCAGGACC ACCAGGACAA 
CCGGGATCTT ATCCATGGGC TTCAAAGCCA ATTGAGATGG AAGTCTTGCA AGGACCTGTC 
GGACCAGCTG GAGTGAAAGG AGAAAAGGGA CGTGATGGAC CAGTAGGACC ACCAGGAATG 
CTCGGACTTG ACGGACCACC AGGATATCCT GGATTAAAGG GACAGAAGGG AGATTTGGGA 
GATGCTGGAC AACGTGGTAA ACGTGGAAAG GACGGAGTTC CAGGAAATTA TGGAGAAAAG 
GGATCCCAAG GAGAACAAGG ACTTGGAGGA ACTCCAGGAT ACCCAGGAAC TAAGGGAGGG 
GCTGGAGAAC CAGGATACCC AGGAAGACCA GGTTTCGAAG GAGACTGTGG ACCGGAAGGA 
CCACTTGGAG AAGGAACTGG TGAGGCTGGA CCACATGGAG CTCAAGGATT CGACGGAGTT 
CAAGGAGGCA AAGGATTGCC AGGACATGAT GGTCTCCCAG GACCAGTAGG TCCAAGAGGT 
CCAGTTGGAG CTCCAGGAGC CCCAGGACAG CCCGGTATCG ATGGAATGCC AGGATACACC 
GAAAAAGGAG ATAGAGGAGA GGACGGTTAC CCAGGATTCG CTGGCGAACC AGGACTCCCA 
GGAGAACCAG GAGATTGTGG TTATCCAGGA GAGGATGGTC TTCCAGGATA CGATATTCAA 
GGACCACCTG GACTTGACGG ACAATCCGGA AGAGATGGAT TCCCTGGTAT TCCAGGAGAC 
ATCGGAGATC CAGGTTATTC TGGAGAAAAG GGATTCCCAG GAACTGGAGT TAACAAAGTT 
GGACCAGACG GATACCCAGG AACACCAGGA GAGCGCGGTA TGGATGGTCT TCCAGGATTC 
CCAGGACTCC ATGGAGAGCC TGGAATGCGT GGACAGCAAG GAGAAGTTGG TTTCAACGGA 
ATTGATGGGG ACTGTGGAGA GCCAGGTCTT GACGGATACC CCGGAGCACC AGGAGCTCCA 
GGAGCTCCAG GAGAGACTGG ATTTGGCTTC CCAGGACAAG TCGGATACCC AGGACCAAAT 
GGAGATGCTG GGGCAGCTGG ACTTCCAGGA CCAGACGGCT ACCCAGGAAG AGACGGTCTT 
CCAGGAACTC CTGGATACCC AGGAGAAGCA GGAATGAACG GACAAGATGG AGCCCCAGGA 
CAGCCAGGAT CTCGTGGAGA GTCTGGACTT GTTGGAATTG ATGGAAAGAA AGGACGCGAT 
GGAACCCCAG GAACACGTGG ACAAGACGGT GGACCGGGAT ATTCCGGAGA GGCTGGAGCC 
CCAGGACAAA ATGGAATGGA TGGATACCCA GGAGCACCAG GAGATCAAGG ATATCCAGGA 
TCCCCAGGGC AAGACGGATA CCCAGGGCCA AGTGGAATTC CAGGAGAGGA CGGTCTTGTC 
GGATTCCCAG GACTTCGTGG TGAACACGGA GACAATGGTC TTCCAGGATT GGAGGGAGAA 
TGCGGAGAGG AAGGATCCAG AGGACTTGAT GGTGTTCCAG GTTACCCAGG AGAACACGGA 
ACCGACGGGC TACCAGGGCT TCCAGGAGCT GATGGTCAAC CAGGTTTTGT TGGAGAAGCC 
GGAGAGCCAG GTACACCAGG ATACAGAGGT CAGCCAGGAG AACCAGGAAA CCTCGCATAT 
CCAGGACAGC CAGGAGATGT TGGATACCCA GGACCAGATG GACCACCAGG TCTTCCAGGA 
CAAGACGGCC TTCCAGGACT GAATGGTGAA CGAGGAGACA ATGGAGATAG TTATCCAGGA 
AACCCAGGAT TGAGCGGCCA ACCAGGAGAT GCTGGATATG ACGGACTTGA TGGAGTCCCA 
GGACCACCAG GATACCCAGG AATCACTGGG ATGCCAGGGC TCAAGGGAGA ATCTGGACTT 
CCAGGACTTC CAGGACGTCA AGGAAATGAC GGCATTCCAG GTCAACCAGG ACTTGAAGGA 
GAATGTGGTG AAGATGGGTT CCCAGGATCT CCAGGGCAAC CAGGTTATCC TGGGCAGCAA 
GGACGTGAAG GAGAGAAGGG ATATCCAGGA ATTCCAGGAG AAAATGGTCT CCCTGGTCTT 
CGTGGACAAG ACGGCCAGCC AGGTCTCAAG GGAGAAAATG GTTTGGATGG TCAGCCAGGT 
TATCCAGGAT CCGCAGGACA ATTGGGAACT CCAGGAGACG TTGGATATCC AGGAGCACCT 
GGAGAAAATG GAGACAACGG AAATCAGGGA CGTGATGGAC AACCTGGACT TCGTGGAGAA 
TCAGGTCAGC CAGGACAGCC AGGATTGCCA GGAAGAGATG GTCAGCCAGG ACCAGTTGGA 
CCACCAGGAG ACGATGGATA CCCAGGAGCA CCCGGACAAG ACATATATGG CCCACCAGGT 
CAAGCTGGAC AAGATGGCTA CCCAGGACTT GATGGACTCC CAGGAGCCCC AGGACTTAAT 
GGAGAACCAG GATCTCCAGG ACAATACGGA ATGCCAGGTC TTCCAGGAGG CCCAGGAGAA 
TCAGGACTAC CAGGATATCC AGGAGAACGT GGTCTTCCCG GACTCGACGG AAAGCGCGGT 
CATGATGGAC TCCCAGGAGC ACCAGGTGTA CCAGGAGTTG AGGGAGTTCC AGGACTTGAA 
GGAGATTGTG GAGAAGACGG TTATCCAGGA GCTCCAGGAG CCCCAGGAAG CAACGGATAT 
CCAGGAGAGC GTGGTCTTCC AGGTGTCCCA GGACAACAAG GACGATCTGG TGACAACGGT 
TATCCAGGAG CACCAGGACA ACCTGGAATC AAGGGACCAC GTGGAGATGA TGGATTCCCA 
GGACGCGACG GACTCGACGG ACTTCCAGGT CGACCAGGAC GTGAAGGATT GCCCGGACCA 
ATGGCTATGG CAGTTAGAAA CCCGCCAGGC CAACCAGGAG AAAATGGTTA TCCAGGAGAG 
AAGGGATACC CAGGACTTCC AGGAGATAAC GGACTTTCCG GACCACCAGG AAAAGCCGGA 
TACCCAGGAG CTCCAGGAAC AGACGGCTAT CCAGGACCAC CAGGTCTTTC AGGAATGCCT 
GGACACGGTG GAGATCAAGG ATTCCAAGGA GCTGCTGGAC GTACTGGTAA TCCAGGACTT 
CCAGGTACTC CAGGATACCC AGGATCACCA GGAGGATGGG CACCAAGTCG AGGATTCACT 
TTTGCCAAGC ACTCTCAGAC TACCGCAGTA CCACAGTGCC CACCAGGAGC TTCTCAACTT 
TGGGAAGGAT ATTCTCTTCT TTACGTTCAA GGAAACGGTC GTGCCAGTGG ACAAGATCTT 
GGTCAACCAG GATCTTGCCT CTCCAAATTC AACACAATGC CATTCATGTT CTGTAATATG 
AACAGCGTCT GCCACGTTTC CAGCCGTAAC GATTACTCGT TCTGGTTGTC AACTGACGAG 
CCAATGACAC CAATGATGAA TCCAGTCACC GGAACAGCAA TTCGTCCATA CATTTCTCGT 
TGTGCCGTCT GTGAAGTTCC AACTCAAATC ATCGCAGTTC ACTCTCAAGA CACATCAGTT 
CCACAATGTC CACAAGGATG GTCGGGTATG TGGACCGGAT ATTCCTTTGT CATGCACACT 
GCCGCTGGAG CCGAAGGAAC TGGACAAAGT CTTCAATCGC CTGGTTCCTG TCTCGAAGAG 
TTCCGTGCCG TTCCATTCAT TGAGTGCCAC GGAAGAGGAA CATGCAATTA TTATGCCACC 
AATCACGGAT TCTGGCTTTC CATCGTTGAT CAGGACAAGC AATTCCGTAA ACCAATGTCA 
CAGACACTCA AAGCTGGAGG ACTTAAAGAT CGTGTATCCA GATGCCAAGT GTGTCTCAAG 
AACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022663.1
CDS	1..4509
Translation	MSRLSLLGLTAAVVLLSSFCQDRIHVDAAAACKGCAPPCVCPGTKGERGNPGFGGEPGHPGAPGQDGPEGAPGAPGMFGAEGDFGDMGSKGARGDRGLPGSPGHPGLQGLDGLPGLKGEEGIPGCNGTDGFPGMPGLAGPPGQSGQNGNPGRPGLSGPPGEGGVNSQGRKGVKGESGRSGVPGLPGNSGYPGLKGAKGDPGPYGLPGFPGVSGLKGRMGVRTSGVKGEKGLPGPPGPPGQPGSYPWASKPIEMEVLQGPVGPAGVKGEKGRDGPVGPPGMLGLDGPPGYPGLKGQKGDLGDAGQRGKRGKDGVPGNYGEKGSQGEQGLGGTPGYPGTKGGAGEPGYPGRPGFEGDCGPEGPLGEGTGEAGPHGAQGFDGVQGGKGLPGHDGLPGPVGPRGPVGAPGAPGQPGIDGMPGYTEKGDRGEDGYPGFAGEPGLPGEPGDCGYPGEDGLPGYDIQGPPGLDGQSGRDGFPGIPGDIGDPGYSGEKGFPGTGVNKVGPDGYPGTPGERGMDGLPGFPGLHGEPGMRGQQGEVGFNGIDGDCGEPGLDGYPGAPGAPGAPGETGFGFPGQVGYPGPNGDAGAAGLPGPDGYPGRDGLPGTPGYPGEAGMNGQDGAPGQPGSRGESGLVGIDGKKGRDGTPGTRGQDGGPGYSGEAGAPGQNGMDGYPGAPGDQGYPGSPGQDGYPGPSGIPGEDGLVGFPGLRGEHGDNGLPGLEGECGEEGSRGLDGVPGYPGEHGTDGLPGLPGADGQPGFVGEAGEPGTPGYRGQPGEPGNLAYPGQPGDVGYPGPDGPPGLPGQDGLPGLNGERGDNGDSYPGNPGLSGQPGDAGYDGLDGVPGPPGYPGITGMPGLKGESGLPGLPGRQGNDGIPGQPGLEGECGEDGFPGSPGQPGYPGQQGREGEKGYPGIPGENGLPGLRGQDGQPGLKGENGLDGQPGYPGSAGQLGTPGDVGYPGAPGENGDNGNQGRDGQPGLRGESGQPGQPGLPGRDGQPGPVGPPGDDGYPGAPGQDIYGPPGQAGQDGYPGLDGLPGAPGLNGEPGSPGQYGMPGLPGGPGESGLPGYPGERGLPGLDGKRGHDGLPGAPGVPGVEGVPGLEGDCGEDGYPGAPGAPGSNGYPGERGLPGVPGQQGRSGDNGYPGAPGQPGIKGPRGDDGFPGRDGLDGLPGRPGREGLPGPMAMAVRNPPGQPGENGYPGEKGYPGLPGDNGLSGPPGKAGYPGAPGTDGYPGPPGLSGMPGHGGDQGFQGAAGRTGNPGLPGTPGYPGSPGGWAPSRGFTFAKHSQTTAVPQCPPGASQLWEGYSLLYVQGNGRASGQDLGQPGSCLSKFNTMPFMFCNMNSVCHVSSRNDYSFWLSTDEPMTPMMNPVTGTAIRPYISRCAVCEVPTQIIAVHSQDTSVPQCPQGWSGMWTGYSFVMHTAAGAEGTGQSLQSPGSCLEEFRAVPFIECHGRGTCNYYATNHGFWLSIVDQDKQFRKPMSQTLKAGGLKDRVSRCQVCLKNR

Target ORF information:

RefSeq Version	NM_001027492.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Collagen alpha-1(IV) chain (emb-9), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027492.4

ORF Insert Sequence:

ATGTCACGCT TATCGCTTCT CGGCTTGACG GCAGCCGTAG TGCTACTGTC GTCGTTCTGT 
CAAGATAGGA TACATGTGGA TGCTGCCGCC GCATGCAAGG GATGTGCTCC ACCATGTGTT 
TGCCCAGGAA CCAAAGGAGA ACGTGGTAAT CCAGGATTTG GTGGTGAACC AGGACATCCT 
GGAGCACCAG GACAAGATGG ACCAGAAGGA GCACCAGGAG CTCCAGGAAT GTTTGGAGCC 
GAAGGAGATT TTGGAGATAT GGGATCTAAG GGAGCTCGTG GAGATCGTGG TCTTCCTGGA 
TCGCCAGGTC ATCCAGGTCT TCAAGGACTT GACGGATTAC CAGGACTGAA AGGAGAAGAA 
GGAATTCCAG GATGCAATGG AACAGATGGT TTCCCTGGAA TGCCCGGACT TGCTGGACCT 
CCAGGGCAAT CTGGACAAAA CGGAAACCCT GGACGACCAG GACTCTCCGG ACCACCAGGA 
GAAGGAGGTG TCAATTCACA AGGACGCAAA GGAGTTAAAG GAGAATCTGG AAGATCAGGA 
GTTCCAGGTC TTCCAGGAAA CTCTGGTTAT CCTGGATTGA AAGGAGCAAA GGGAGATCCA 
GGACCGTACG GTCTTCCCGG ATTCCCCGGA GTTTCTGGAT TGAAGGGAAG AATGGGAGTG 
AGAACTTCTG GTGTTAAGGG AGAGAAGGGT TTGCCTGGAC CACCAGGACC ACCAGGACAA 
CCGGGATCTT ATCCATGGGC TTCAAAGCCA ATTGAGATGG AAGTCTTGCA AGGACCTGTC 
GGACCAGCTG GAGTGAAAGG AGAAAAGGGA CGTGATGGAC CAGTAGGACC ACCAGGAATG 
CTCGGACTTG ACGGACCACC AGGATATCCT GGATTAAAGG GACAGAAGGG AGATTTGGGA 
GATGCTGGAC AACGTGGTAA ACGTGGAAAG GACGGAGTTC CAGGAAATTA TGGAGAAAAG 
GGATCCCAAG GAGAACAAGG ACTTGGAGGA ACTCCAGGAT ACCCAGGAAC TAAGGGAGGG 
GCTGGAGAAC CAGGATACCC AGGAAGACCA GGTTTCGAAG GAGACTGTGG ACCGGAAGGA 
CCACTTGGAG AAGGAACTGG TGAGGCTGGA CCACATGGAG CTCAAGGATT CGACGGAGTT 
CAAGGAGGCA AAGGATTGCC AGGACATGAT GGTCTCCCAG GACCAGTAGG TCCAAGAGGT 
CCAGTTGGAG CTCCAGGAGC CCCAGGACAG CCCGGTATCG ATGGAATGCC AGGATACACC 
GAAAAAGGAG ATAGAGGAGA GGACGGTTAC CCAGGATTCG CTGGCGAACC AGGACTCCCA 
GGAGAACCAG GAGATTGTGG TTATCCAGGA GAGGATGGTC TTCCAGGATA CGATATTCAA 
GGACCACCTG GACTTGACGG ACAATCCGGA AGAGATGGAT TCCCTGGTAT TCCAGGAGAC 
ATCGGAGATC CAGGTTATTC TGGAGAAAAG GGATTCCCAG GAACTGGAGT TAACAAAGTT 
GGACCAGACG GATACCCAGG AACACCAGGA GAGCGCGGTA TGGATGGTCT TCCAGGATTC 
CCAGGACTCC ATGGAGAGCC TGGAATGCGT GGACAGCAAG GAGAAGTTGG TTTCAACGGA 
ATTGATGGGG ACTGTGGAGA GCCAGGTCTT GACGGATACC CCGGAGCACC AGGAGCTCCA 
GGAGCTCCAG GAGAGACTGG ATTTGGCTTC CCAGGACAAG TCGGATACCC AGGACCAAAT 
GGAGATGCTG GGGCAGCTGG ACTTCCAGGA CCAGACGGCT ACCCAGGAAG AGACGGTCTT 
CCAGGAACTC CTGGATACCC AGGAGAAGCA GGAATGAACG GACAAGATGG AGCCCCAGGA 
CAGCCAGGAT CTCGTGGAGA GTCTGGACTT GTTGGAATTG ATGGAAAGAA AGGACGCGAT 
GGAACCCCAG GAACACGTGG ACAAGACGGT GGACCGGGAT ATTCCGGAGA GGCTGGAGCC 
CCAGGACAAA ATGGAATGGA TGGATACCCA GGAGCACCAG GAGATCAAGG ATATCCAGGA 
TCCCCAGGGC AAGACGGATA CCCAGGGCCA AGTGGAATTC CAGGAGAGGA CGGTCTTGTC 
GGATTCCCAG GACTTCGTGG TGAACACGGA GACAATGGTC TTCCAGGATT GGAGGGAGAA 
TGCGGAGAGG AAGGATCCAG AGGACTTGAT GGTGTTCCAG GTTACCCAGG AGAACACGGA 
ACCGACGGGC TACCAGGGCT TCCAGGAGCT GATGGTCAAC CAGGTTTTGT TGGAGAAGCC 
GGAGAGCCAG GTACACCAGG ATACAGAGGT CAGCCAGGAG AACCAGGAAA CCTCGCATAT 
CCAGGACAGC CAGGAGATGT TGGATACCCA GGACCAGATG GACCACCAGG TCTTCCAGGA 
CAAGACGGCC TTCCAGGACT GAATGGTGAA CGAGGAGACA ATGGAGATAG TTATCCAGGA 
AACCCAGGAT TGAGCGGCCA ACCAGGAGAT GCTGGATATG ACGGACTTGA TGGAGTCCCA 
GGACCACCAG GATACCCAGG AATCACTGGG ATGCCAGGGC TCAAGGGAGA ATCTGGACTT 
CCAGGACTTC CAGGACGTCA AGGAAATGAC GGCATTCCAG GTCAACCAGG ACTTGAAGGA 
GAATGTGGTG AAGATGGGTT CCCAGGATCT CCAGGGCAAC CAGGTTATCC TGGGCAGCAA 
GGACGTGAAG GAGAGAAGGG ATATCCAGGA ATTCCAGGAG AAAATGGTCT CCCTGGTCTT 
CGTGGACAAG ACGGCCAGCC AGGTCTCAAG GGAGAAAATG GTTTGGATGG TCAGCCAGGT 
TATCCAGGAT CCGCAGGACA ATTGGGAACT CCAGGAGACG TTGGATATCC AGGAGCACCT 
GGAGAAAATG GAGACAACGG AAATCAGGGA CGTGATGGAC AACCTGGACT TCGTGGAGAA 
TCAGGTCAGC CAGGACAGCC AGGATTGCCA GGAAGAGATG GTCAGCCAGG ACCAGTTGGA 
CCACCAGGAG ACGATGGATA CCCAGGAGCA CCCGGACAAG ACATATATGG CCCACCAGGT 
CAAGCTGGAC AAGATGGCTA CCCAGGACTT GATGGACTCC CAGGAGCCCC AGGACTTAAT 
GGAGAACCAG GATCTCCAGG ACAATACGGA ATGCCAGGTC TTCCAGGAGG CCCAGGAGAA 
TCAGGACTAC CAGGATATCC AGGAGAACGT GGTCTTCCCG GACTCGACGG AAAGCGCGGT 
CATGATGGAC TCCCAGGAGC ACCAGGTGTA CCAGGAGTTG AGGGAGTTCC AGGACTTGAA 
GGAGATTGTG GAGAAGACGG TTATCCAGGA GCTCCAGGAG CCCCAGGAAG CAACGGATAT 
CCAGGAGAGC GTGGTCTTCC AGGTGTCCCA GGACAACAAG GACGATCTGG TGACAACGGT 
TATCCAGGAG CACCAGGACA ACCTGGAATC AAGGGACCAC GTGGAGATGA TGGATTCCCA 
GGACGCGACG GACTCGACGG ACTTCCAGGT CGACCAGGAC GTGAAGGATT GCCCGGACCA 
ATGGCTATGG CAGTTAGAAA CCCGCCAGGC CAACCAGGAG AAAATGGTTA TCCAGGAGAG 
AAGGGATACC CAGGACTTCC AGGAGATAAC GGACTTTCCG GACCACCAGG AAAAGCCGGA 
TACCCAGGAG CTCCAGGAAC AGACGGCTAT CCAGGACCAC CAGGTCTTTC AGGAATGCCT 
GGACACGGTG GAGATCAAGG ATTCCAAGGA GCTGCTGGAC GTACTGGTAA TCCAGGACTT 
CCAGGTACTC CAGGATACCC AGGATCACCA GGAGGATGGG CACCAAGTCG AGGATTCACT 
TTTGCCAAGC ACTCTCAGAC TACCGCAGTA CCACAGTGCC CACCAGGAGC TTCTCAACTT 
TGGGAAGGAT ATTCTCTTCT TTACGTTCAA GGAAACGGTC GTGCCAGTGG ACAAGATCTT 
GGTCAACCAG GATCTTGCCT CTCCAAATTC AACACAATGC CATTCATGTT CTGTAATATG 
AACAGCGTCT GCCACGTTTC CAGCCGTAAC GATTACTCGT TCTGGTTGTC AACTGACGAG 
CCAATGACAC CAATGATGAA TCCAGTCACC GGAACAGCAA TTCGTCCATA CATTTCTCGT 
TGTGCCGTCT GTGAAGTTCC AACTCAAATC ATCGCAGTTC ACTCTCAAGA CACATCAGTT 
CCACAATGTC CACAAGGATG GTCGGGTATG TGGACCGGAT ATTCCTTTGT CATGCACACT 
GCCGCTGGAG CCGAAGGAAC TGGACAAAGT CTTCAATCGC CTGGTTCCTG TCTCGAAGAG 
TTCCGTGCCG TTCCATTCAT TGAGTGCCAC GGAAGAGGAA CATGCAATTA TTATGCCACC 
AATCACGGAT TCTGGCTTTC CATCGTTGAT CAGGACAAGC AATTCCGTAA ACCAATGTCA 
CAGACACTCA AAGCTGGAGG ACTTAAAGAT CGTGTATCCA GATGCCAAGT GTGTCTCAAG 
AACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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emb-9 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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