col4a2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	col4a2
Entrez Gene ID	100486079
Full Name	collagen, type IV, alpha 2
General protein information	Preferred Names collagen, type IV, alpha 2 Names collagen alpha-2(IV) chain
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004911987.4	XP_004912044.1	collagen alpha-2(IV) chain
XM_004911987.3	XP_004912044.1	collagen alpha-2(IV) chain
XM_002933017.2	XP_002933063.2	collagen alpha-2(IV) chain
XM_002933017.3	XP_002933063.2	collagen alpha-2(IV) chain
XM_031897040.1	XP_031752900.1	collagen alpha-2(IV) chain
XM_031897041.1	XP_031752901.1	collagen alpha-2(IV) chain

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL4A2	XP_001136859.1
Canis lupus familiaris (dog)	COL4A2	XP_534183.3
Rattus norvegicus (Norway rat)	Col4a2	XP_006253538.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009200	XP_319976.3
	COL4A2	XP_002800878.1
Drosophila melanogaster (fruit fly)	Cg25C	NP_723046.1
Caenorhabditis elegans (roundworm)	emb-9	NP_001022662.1
Bos taurus (cattle)	COL4A2	XP_002692057.2
Mus musculus (house mouse)	Col4a2	NP_034062.3
Gallus gallus (chicken)	COL4A2	NP_001155862.1
Danio rerio (zebrafish)	col4a2	XP_687811.6
Caenorhabditis elegans (roundworm)	let-2	NP_510663.1
Xenopus tropicalis (tropical clawed frog)	col4a2	XP_004912045.1
Homo sapiens (human)	COL4A2	NP_001837.2

Gene Ontology

The following col4a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col4a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa06182	XM_004911987.4 Latest version!	Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa06182	XM_002933017.3 Latest version!	Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa06182	XM_031897041.1 Latest version!	Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa06182	XM_031897040.1 Latest version!	Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OXa06182	XM_004911987.3	Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OXa06182	XM_002933017.2	Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col4a2 ( XM_004911987.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912044.1 Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X1, mRNA.

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CloneID	OXa06182
Clone ID Related Accession (Same CDS sequence)	XM_004911987.4 , XM_004911987.3 , XM_002933017.2 , XM_002933017.3 , XM_031897040.1 , XM_031897041.1
Accession Version	XM_004911987.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-2(IV) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911987.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA CTCAAAGCAC CAAGAGATGT CCCACAAGCA GGAGAGAGTA CAGCTGTTTT 
CAGCTCCAAC ACTATTCAGT CACTTGGTGC ATGCTGTTTG TTGTACTGGT GACATGCTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGCC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGAT GCCAGTGTCT GCCCGAGAAA GGAAGTCGTG GACAACCAGG ACCCCTGGGA 
GGGCAAGGTA CCTCTGGCCC ACCTGGGCTA ATAGGAATAG CAGGTCTGCA GGGACGTAAG 
GGAGACAAAG GAGAACGAGG ATTTCCTGGA GTGACTGGCC CCTCTGGAGA TAAGGGCCAA 
AGTGGTGTGA CAGGTTTTCC CGGAGCTGAT GGCGTACCTG GACATACAGG TCAGGGTGGC 
CCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAC AAGGCGATGC TGGTGTGGGA 
GGTTCACATG GTTCAAGAGG CCCTCCTGGA ATTTCTGGTG GATTTGGTGC CAAAGGTCAA 
AAAGGAGAAC CGTTTCATGT ATCAATCGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAAGCAGGCT TTAATGGTAT TCAGGGTCCA AGGGGTTCTC CTGGAACAGC AGGCTTCATT 
GGGCCACAGG GTTACCGTGG ACCTCGTGGG CCACCTGGAC CTCCTGGGCC ACAGGGACCA 
CAAGGCAACA GGGGTCTTGG ATATTATGGA GAAAAAGGAG AACCTGGTGA TCCTGGTCCA 
CCTGGTGAAC AACCTCGTCA AGGAGAGAGA GAACAAAAGC ACAAAATTGA AATTCTCTTG 
CTACAATATA AGGGGGCCAA GGGGGACCAA GGAGAGAAGG GAAATCCTGG CGATAAAGGT 
CAACCAGCTG TCACAGGAGA AAAAGGAGAG GAAGGTATTA CAGGATTTCC TGGACAAAGG 
GGGTTTCCTG GTAATGATGG TAACAGTGGA GCTTCAGGAG AAATAGGGTT TCCTGGGGCA 
AACGGTGTAC CTGGATTACC TGGTACTAAA GGAAAAAAGG GAGAGGTTGG AGACCTAGGA 
CCTCAAGGAC CTGTTACTTT TGTCAATGGT CAACAAAAAA GAAAAGGCTA TGAAGGTGAA 
ATTGGATTTC CAGGACTTAA TGGGGTTTAT GGAGATGATG GAGACCCTGG AGATCCTGGT 
TTTCCAGGCC AGAATGGATC ACCAAGTAGA TTTCCAGGAC AAACAGGTGA TACAGGCTTG 
AAAGGTCAAA GGGGTCCCAA AGGTTACCAA GGTGATACAG GCTTACCAGC ATTTCAGCCT 
GGACCCCCAG GTATAGATGG AGCCCCTGGC AAAGAAGGAC CACCTGGCCA ACCAGGTTAT 
CCAGGTGGAC GCATTGGGAT GAAGGGATTA CCCGGGTTTC CTGGAAGGGA TGGACAAAGA 
GGAGCTCCTG GTTCTCGAGG TTTAAGGGGA ATCAAAGGTG AACAAGGGGA ATGCAGATGC 
TATGAAGGAG ATGAAGCTGG CAGAGGCCCT GCTGGAGAGC CTGGTCCAGT TGGTTTTCCA 
GGTTTTCAGG GTCACGCAGG CAGAAAGGGA GAAGCAGGAG ATAGAGGAGT ACAAGGGCTT 
CCAGGAGCAC CAGGCACAGT GGGTTTAGCT GGTCCTGCTG GGTTTCCTGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTATT TGCTACAGAA AAAGGTTCCA AGGGAGCACA AGGAGACTCT 
GGTTTTCCTG GTGTTCCTGG GCGTGATGGA TACCCTGGAA GAAATGGTAG AGATGGTAGT 
CCTGGCTTTC CAGGCCCTTC TGGAGATGGT ATAAAAGGAC TCCCAGGTTC TCATGGGTTT 
CCAGGACTCC CTGGTAATGC TGGTGCTCGA GGTGAGACGG GCTTTCCTGG ACCAGGATTT 
CCAGGTTCAA AAGGGCTGCG GGGAGAGCCA GGAAACAGAG GAGTGAGCGG ATTTCCAGGA 
GTTCCTGGAC TTTCAGGCCC CCAAGGTGAC TGCTATCAAG AAACTGATGA AGAAAATGAA 
ATAGGGAAAG AAGGGACTGG CTGCAGACTA ATTGCACCTC CTGCTCCAGC AGGTGCCCCA 
GGGCCACCAG GATTACCAGG ATATAACGGT ATAAGAGGCC AGAAAGGTTT TCCCGGACCA 
CATGGTTTTG ATGGCGTTTT TGGATTAAAA GGCATCAAAG GAGACCGTGG AACCGATAGT 
TTGCCAGGTC CACCAGGTTT TTCTGGACAA AGGGGAGACA GAGGTAATGC AGGACTACCT 
GGCCTACCCG GGGTACCAGG TTTGTCAGGA AAACCTGGCT TTAAAGGGGT ACAAGGAGAA 
AAAGGAAGTG TAGGTGATCG CTTAGGAGCT CCATCAGGGG AGCAAGGAAA TACTGGCCTG 
CCTGGCTTGC AAGGACTTAA AGGAACACAA GGGGATCAAG GCATACCTGG CCTACAAGGT 
AAAGTTGGAA CACCAGGAAC ACCTGGAAAC AAGGGTATTA AAGGCTCTCC CGGGGCCCCA 
GGAGTACCTG GAATACCAGG ACCTGCAGCA TTATTTGGAT TTCCAGGAAT GCCCGGAAAC 
CCAGGCACAC CAGGACCCCA AGGTTCAATG GGACCTCCAG GTCCTCCAGG ACAGAGAGGA 
GATGAAGGCG AGAAAGGGCC ACCAGGTCCA GCTGGGATGA AAGGGTTACC TGGAGAACTT 
GGTGTTGGAG GATTTATTGG TGTTAGGGGC AGACATGGAA ATCCAGGCAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGCATCAAAG GTTCCCCTGG CATGGAAGGC 
TTCCAGGGAA TGAAAGGAGC GAAGGGGAGA CCTGCATGGA GAGGACTCAA AGGACCAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG GGAAATGGAA CTTCCGGTAC AAAAGGTGAC 
CGTGGGGAAC AAGGACCACC AGGAGACCCC CCCAAAATGC CAGAAATGAT GCTTTTGACA 
AAAGGCGAAA CAGGAGATCA AGGTGTCTCG GGCTTTAAAG GAATTTCTGG ACTTAAAGGC 
AGTAAAGGTA TGCCTGGACC TCCAGGACAG CTAGGCTTAC CTGGCTTGCC TGGACTACGA 
AGCTTTGAAT TTGGAGATAA AGGAGAAACT GGTACTCCTG GGGTTATCGG TAATCAAGGA 
ATTCTAGGAG AAGTTGGTCC AGACGGAATT ATTGGCTTCC CAGGATTTAC TGGTCCCAGA 
GGTACTCCTG GTATTTCTGG CTTTCCTGGT GTTCCTGGTG AAAGAGGCAA ATATGGAGAT 
ATAGGAGAAA GAGGTGATAC TATTGATTTA CCAGGAGAAA GAGGAACAAA GGGTCAGGGT 
GGAACCCCCG GGCTGCCAGG ACAGCGAGGA ATACACGGAG AAAAAGGAAC TACAGGAGAT 
GAAGGTTTTC GGGGTATTGA GGGAGTGATA GGGGAACACG GACAAACAGG AGAAAAAGGA 
TTCCCAGGTC AACAAGGCCT TGTGGGCTTT CCAGGCTCAC AGGGTTTTCC AGGTCTGCCA 
GGTGTGCCTG GCGAGAAAGG AAGTTCTGGG TTTCCTGGGT TACCTGGTCA ACATGGTTTT 
CCTGGCATAA GAGGGATTGC TGGCCTAGAT GGTCTGCCAG GCACTAAAGG AATTAATGGA 
CAGCCAGGTG CGGATATAAT TGGTTTAAAG GGATTTAAAG GTCTAAGTGG TGGAAAGGGT 
CAACCAGGAG AGGCTAGTAC CATTGTAGGA ATGCCTGGAC CTGCAGGATC TAAAGGAGCC 
GAAGGGGATC AAGGTTTACC TGGCTTAATT GGACTTCCTG GAACAGTTGG AGTCCGTGGC 
TTGCCTGGGT TTTCTAACAA GACAGGTTTG CTAGGAGATG TTGGACCTCC AGGACCTCCT 
GGACTGCCAG GTTTTCCAGG CCCTATCGGA AGACCAGGTT TACCCTCTAA ACCTGGAGTA 
AAAGGAGTCA TAGGGGATCT GGGGTTACAA GGGAACTATG GTGCTAAAGG TATTCCTGGT 
GACGAAGGAA AAGTGGGAGT TCCTGGACTG GCTGGAATAC CTGGAGCCAA AGGTTTGAAA 
GGCAGTGCTG GCTTCCAGGG TTTTGTTGGG GTATCAGGGT ATCGAGGAGA TCAAGGACCT 
AAAGGGCTTA GAGGTGACAT CGGAGAATAT GGTTTAAAGG GTCCTCCTGG ACAACCGGGC 
CCTATGTCTG AACCTCCTCT TATTACGGCT GAACAAGGCT ACCCTGGAGC ACACGGAATT 
ATTGGCCATC AAGGTGTTCA GGGCGATATG GGCCCAAAAG GTCTTGTGGG TACTCCAGGT 
GCCCCTGGCC AAACCGGTAA AGAAGGCTTA CCAGGTGTAC CAGGCTGGCC TGCTCCTCCA 
GGCAGTCGTG GAGAACAAGG ACCCATGGGA CGAACAGGAC CTAATGGATT CAAAGGTGCC 
AGTGGTGTGC CAGGCAGGGC AGGGCTTCCT GGGATGCCCG GGAGAAGTGT CAATATTGGC 
TATCTTTTAG TCAAGCACAG TCAAACAGAT GAAGAGCCAA TGTGTCCTGT TGGTATGGCC 
CGCTTGTGGA CTGGGTATAG TCTCTTGTAT TTTGAAGGAC AGGAAAAAGC ACACAATCAA 
GACTTAGGTC TAGCTGGATC TTGTTTACAG CGCTTCAGTA CAATGCCGTT CCTGTACTGC 
AACCCAGGGG ATGTCTGTTA TTATGCCAAC CGTAACGATA AGTCTTATTG GCTTTCCACC 
ACTGCACCTC TTCCAATGAT GCCTGTGGTT GAAGAAGAAA TTAGGCCATA TATTAGCCGT 
TGCTCAGTGT GTGAGGCTCC AGCAGTAGCA ATTGCTGTTC ACAGTCAAGA TGTCAGTATC 
CCACATTGCC CAGATGGTTG GCGGAGCTTG TGGATTGGCT ACTCCTTTCT CATGCACACT 
GCCGCAGGCG ATGAGGGGGG AGGACAATCT CTCTCCTCCC CTGGAAGTTG TTTGGAAGAC 
TTCAGGGCGA CTCCATTCAT TGAATGCAAT GGTGGAAGAG GGACCTGCCA CTACTTTGCA 
AACAAATACA GTTTCTGGCT TACTACTATT GACGAGCCCT TCCAGAGCTC TCCGCCTGCA 
GATACACTAA AAGCTGGGCT GATTCGAACT CATATCAGCC GATGCCAAGT CTGCATGAAA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912044.1
CDS	182..5350
Translation	MAGTQSTKRCPTSRREYSCFQLQHYSVTWCMLFVVLVTCFAGNAHAGDKSYTGPCGGRDCSQGCQCLPEKGSRGQPGPLGGQGTSGPPGLIGIAGLQGRKGDKGERGFPGVTGPSGDKGQSGVTGFPGADGVPGHTGQGGPRGKPGHDGCNGTQGDAGVGGSHGSRGPPGISGGFGAKGQKGEPFHVSIEVKNRLRGDPGEAGFNGIQGPRGSPGTAGFIGPQGYRGPRGPPGPPGPQGPQGNRGLGYYGEKGEPGDPGPPGEQPRQGEREQKHKIEILLLQYKGAKGDQGEKGNPGDKGQPAVTGEKGEEGITGFPGQRGFPGNDGNSGASGEIGFPGANGVPGLPGTKGKKGEVGDLGPQGPVTFVNGQQKRKGYEGEIGFPGLNGVYGDDGDPGDPGFPGQNGSPSRFPGQTGDTGLKGQRGPKGYQGDTGLPAFQPGPPGIDGAPGKEGPPGQPGYPGGRIGMKGLPGFPGRDGQRGAPGSRGLRGIKGEQGECRCYEGDEAGRGPAGEPGPVGFPGFQGHAGRKGEAGDRGVQGLPGAPGTVGLAGPAGFPGPAGEKGDKVFATEKGSKGAQGDSGFPGVPGRDGYPGRNGRDGSPGFPGPSGDGIKGLPGSHGFPGLPGNAGARGETGFPGPGFPGSKGLRGEPGNRGVSGFPGVPGLSGPQGDCYQETDEENEIGKEGTGCRLIAPPAPAGAPGPPGLPGYNGIRGQKGFPGPHGFDGVFGLKGIKGDRGTDSLPGPPGFSGQRGDRGNAGLPGLPGVPGLSGKPGFKGVQGEKGSVGDRLGAPSGEQGNTGLPGLQGLKGTQGDQGIPGLQGKVGTPGTPGNKGIKGSPGAPGVPGIPGPAALFGFPGMPGNPGTPGPQGSMGPPGPPGQRGDEGEKGPPGPAGMKGLPGELGVGGFIGVRGRHGNPGIPGLVGMHGLPGVKGIKGSPGMEGFQGMKGAKGRPAWRGLKGPSGNIGFPGVKGGNGTSGTKGDRGEQGPPGDPPKMPEMMLLTKGETGDQGVSGFKGISGLKGSKGMPGPPGQLGLPGLPGLRSFEFGDKGETGTPGVIGNQGILGEVGPDGIIGFPGFTGPRGTPGISGFPGVPGERGKYGDIGERGDTIDLPGERGTKGQGGTPGLPGQRGIHGEKGTTGDEGFRGIEGVIGEHGQTGEKGFPGQQGLVGFPGSQGFPGLPGVPGEKGSSGFPGLPGQHGFPGIRGIAGLDGLPGTKGINGQPGADIIGLKGFKGLSGGKGQPGEASTIVGMPGPAGSKGAEGDQGLPGLIGLPGTVGVRGLPGFSNKTGLLGDVGPPGPPGLPGFPGPIGRPGLPSKPGVKGVIGDLGLQGNYGAKGIPGDEGKVGVPGLAGIPGAKGLKGSAGFQGFVGVSGYRGDQGPKGLRGDIGEYGLKGPPGQPGPMSEPPLITAEQGYPGAHGIIGHQGVQGDMGPKGLVGTPGAPGQTGKEGLPGVPGWPAPPGSRGEQGPMGRTGPNGFKGASGVPGRAGLPGMPGRSVNIGYLLVKHSQTDEEPMCPVGMARLWTGYSLLYFEGQEKAHNQDLGLAGSCLQRFSTMPFLYCNPGDVCYYANRNDKSYWLSTTAPLPMMPVVEEEIRPYISRCSVCEAPAVAIAVHSQDVSIPHCPDGWRSLWIGYSFLMHTAAGDEGGGQSLSSPGSCLEDFRATPFIECNGGRGTCHYFANKYSFWLTTIDEPFQSSPPADTLKAGLIRTHISRCQVCMKNL

Target ORF information:

RefSeq Version	XM_004911987.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911987.4

ORF Insert Sequence:

ATGGCAGGAA CTCAAAGCAC CAAGAGATGT CCCACAAGCA GGAGAGAGTA CAGCTGTTTT 
CAGCTCCAAC ACTATTCAGT CACTTGGTGC ATGCTGTTTG TTGTACTGGT GACATGCTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGCC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGAT GCCAGTGTCT GCCCGAGAAA GGAAGTCGTG GACAACCAGG ACCCCTGGGA 
GGGCAAGGTA CCTCTGGCCC ACCTGGGCTA ATAGGAATAG CAGGTCTGCA GGGACGTAAG 
GGAGACAAAG GAGAACGAGG ATTTCCTGGA GTGACTGGCC CCTCTGGAGA TAAGGGCCAA 
AGTGGTGTGA CAGGTTTTCC CGGAGCTGAT GGCGTACCTG GACATACAGG TCAGGGTGGC 
CCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAC AAGGCGATGC TGGTGTGGGA 
GGTTCACATG GTTCAAGAGG CCCTCCTGGA ATTTCTGGTG GATTTGGTGC CAAAGGTCAA 
AAAGGAGAAC CGTTTCATGT ATCAATCGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAAGCAGGCT TTAATGGTAT TCAGGGTCCA AGGGGTTCTC CTGGAACAGC AGGCTTCATT 
GGGCCACAGG GTTACCGTGG ACCTCGTGGG CCACCTGGAC CTCCTGGGCC ACAGGGACCA 
CAAGGCAACA GGGGTCTTGG ATATTATGGA GAAAAAGGAG AACCTGGTGA TCCTGGTCCA 
CCTGGTGAAC AACCTCGTCA AGGAGAGAGA GAACAAAAGC ACAAAATTGA AATTCTCTTG 
CTACAATATA AGGGGGCCAA GGGGGACCAA GGAGAGAAGG GAAATCCTGG CGATAAAGGT 
CAACCAGCTG TCACAGGAGA AAAAGGAGAG GAAGGTATTA CAGGATTTCC TGGACAAAGG 
GGGTTTCCTG GTAATGATGG TAACAGTGGA GCTTCAGGAG AAATAGGGTT TCCTGGGGCA 
AACGGTGTAC CTGGATTACC TGGTACTAAA GGAAAAAAGG GAGAGGTTGG AGACCTAGGA 
CCTCAAGGAC CTGTTACTTT TGTCAATGGT CAACAAAAAA GAAAAGGCTA TGAAGGTGAA 
ATTGGATTTC CAGGACTTAA TGGGGTTTAT GGAGATGATG GAGACCCTGG AGATCCTGGT 
TTTCCAGGCC AGAATGGATC ACCAAGTAGA TTTCCAGGAC AAACAGGTGA TACAGGCTTG 
AAAGGTCAAA GGGGTCCCAA AGGTTACCAA GGTGATACAG GCTTACCAGC ATTTCAGCCT 
GGACCCCCAG GTATAGATGG AGCCCCTGGC AAAGAAGGAC CACCTGGCCA ACCAGGTTAT 
CCAGGTGGAC GCATTGGGAT GAAGGGATTA CCCGGGTTTC CTGGAAGGGA TGGACAAAGA 
GGAGCTCCTG GTTCTCGAGG TTTAAGGGGA ATCAAAGGTG AACAAGGGGA ATGCAGATGC 
TATGAAGGAG ATGAAGCTGG CAGAGGCCCT GCTGGAGAGC CTGGTCCAGT TGGTTTTCCA 
GGTTTTCAGG GTCACGCAGG CAGAAAGGGA GAAGCAGGAG ATAGAGGAGT ACAAGGGCTT 
CCAGGAGCAC CAGGCACAGT GGGTTTAGCT GGTCCTGCTG GGTTTCCTGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTATT TGCTACAGAA AAAGGTTCCA AGGGAGCACA AGGAGACTCT 
GGTTTTCCTG GTGTTCCTGG GCGTGATGGA TACCCTGGAA GAAATGGTAG AGATGGTAGT 
CCTGGCTTTC CAGGCCCTTC TGGAGATGGT ATAAAAGGAC TCCCAGGTTC TCATGGGTTT 
CCAGGACTCC CTGGTAATGC TGGTGCTCGA GGTGAGACGG GCTTTCCTGG ACCAGGATTT 
CCAGGTTCAA AAGGGCTGCG GGGAGAGCCA GGAAACAGAG GAGTGAGCGG ATTTCCAGGA 
GTTCCTGGAC TTTCAGGCCC CCAAGGTGAC TGCTATCAAG AAACTGATGA AGAAAATGAA 
ATAGGGAAAG AAGGGACTGG CTGCAGACTA ATTGCACCTC CTGCTCCAGC AGGTGCCCCA 
GGGCCACCAG GATTACCAGG ATATAACGGT ATAAGAGGCC AGAAAGGTTT TCCCGGACCA 
CATGGTTTTG ATGGCGTTTT TGGATTAAAA GGCATCAAAG GAGACCGTGG AACCGATAGT 
TTGCCAGGTC CACCAGGTTT TTCTGGACAA AGGGGAGACA GAGGTAATGC AGGACTACCT 
GGCCTACCCG GGGTACCAGG TTTGTCAGGA AAACCTGGCT TTAAAGGGGT ACAAGGAGAA 
AAAGGAAGTG TAGGTGATCG CTTAGGAGCT CCATCAGGGG AGCAAGGAAA TACTGGCCTG 
CCTGGCTTGC AAGGACTTAA AGGAACACAA GGGGATCAAG GCATACCTGG CCTACAAGGT 
AAAGTTGGAA CACCAGGAAC ACCTGGAAAC AAGGGTATTA AAGGCTCTCC CGGGGCCCCA 
GGAGTACCTG GAATACCAGG ACCTGCAGCA TTATTTGGAT TTCCAGGAAT GCCCGGAAAC 
CCAGGCACAC CAGGACCCCA AGGTTCAATG GGACCTCCAG GTCCTCCAGG ACAGAGAGGA 
GATGAAGGCG AGAAAGGGCC ACCAGGTCCA GCTGGGATGA AAGGGTTACC TGGAGAACTT 
GGTGTTGGAG GATTTATTGG TGTTAGGGGC AGACATGGAA ATCCAGGCAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGCATCAAAG GTTCCCCTGG CATGGAAGGC 
TTCCAGGGAA TGAAAGGAGC GAAGGGGAGA CCTGCATGGA GAGGACTCAA AGGACCAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG GGAAATGGAA CTTCCGGTAC AAAAGGTGAC 
CGTGGGGAAC AAGGACCACC AGGAGACCCC CCCAAAATGC CAGAAATGAT GCTTTTGACA 
AAAGGCGAAA CAGGAGATCA AGGTGTCTCG GGCTTTAAAG GAATTTCTGG ACTTAAAGGC 
AGTAAAGGTA TGCCTGGACC TCCAGGACAG CTAGGCTTAC CTGGCTTGCC TGGACTACGA 
AGCTTTGAAT TTGGAGATAA AGGAGAAACT GGTACTCCTG GGGTTATCGG TAATCAAGGA 
ATTCTAGGAG AAGTTGGTCC AGACGGAATT ATTGGCTTCC CAGGATTTAC TGGTCCCAGA 
GGTACTCCTG GTATTTCTGG CTTTCCTGGT GTTCCTGGTG AAAGAGGCAA ATATGGAGAT 
ATAGGAGAAA GAGGTGATAC TATTGATTTA CCAGGAGAAA GAGGAACAAA GGGTCAGGGT 
GGAACCCCCG GGCTGCCAGG ACAGCGAGGA ATACACGGAG AAAAAGGAAC TACAGGAGAT 
GAAGGTTTTC GGGGTATTGA GGGAGTGATA GGGGAACACG GACAAACAGG AGAAAAAGGA 
TTCCCAGGTC AACAAGGCCT TGTGGGCTTT CCAGGCTCAC AGGGTTTTCC AGGTCTGCCA 
GGTGTGCCTG GCGAGAAAGG AAGTTCTGGG TTTCCTGGGT TACCTGGTCA ACATGGTTTT 
CCTGGCATAA GAGGGATTGC TGGCCTAGAT GGTCTGCCAG GCACTAAAGG AATTAATGGA 
CAGCCAGGTG CGGATATAAT TGGTTTAAAG GGATTTAAAG GTCTAAGTGG TGGAAAGGGT 
CAACCAGGAG AGGCTAGTAC CATTGTAGGA ATGCCTGGAC CTGCAGGATC TAAAGGAGCC 
GAAGGGGATC AAGGTTTACC TGGCTTAATT GGACTTCCTG GAACAGTTGG AGTCCGTGGC 
TTGCCTGGGT TTTCTAACAA GACAGGTTTG CTAGGAGATG TTGGACCTCC AGGACCTCCT 
GGACTGCCAG GTTTTCCAGG CCCTATCGGA AGACCAGGTT TACCCTCTAA ACCTGGAGTA 
AAAGGAGTCA TAGGGGATCT GGGGTTACAA GGGAACTATG GTGCTAAAGG TATTCCTGGT 
GACGAAGGAA AAGTGGGAGT TCCTGGACTG GCTGGAATAC CTGGAGCCAA AGGTTTGAAA 
GGCAGTGCTG GCTTCCAGGG TTTTGTTGGG GTATCAGGGT ATCGAGGAGA TCAAGGACCT 
AAAGGGCTTA GAGGTGACAT CGGAGAATAT GGTTTAAAGG GTCCTCCTGG ACAACCGGGC 
CCTATGTCTG AACCTCCTCT TATTACGGCT GAACAAGGCT ACCCTGGAGC ACACGGAATT 
ATTGGCCATC AAGGTGTTCA GGGCGATATG GGCCCAAAAG GTCTTGTGGG TACTCCAGGT 
GCCCCTGGCC AAACCGGTAA AGAAGGCTTA CCAGGTGTAC CAGGCTGGCC TGCTCCTCCA 
GGCAGTCGTG GAGAACAAGG ACCCATGGGA CGAACAGGAC CTAATGGATT CAAAGGTGCC 
AGTGGTGTGC CAGGCAGGGC AGGGCTTCCT GGGATGCCCG GGAGAAGTGT CAATATTGGC 
TATCTTTTAG TCAAGCACAG TCAAACAGAT GAAGAGCCAA TGTGTCCTGT TGGTATGGCC 
CGCTTGTGGA CTGGGTATAG TCTCTTGTAT TTTGAAGGAC AGGAAAAAGC ACACAATCAA 
GACTTAGGTC TAGCTGGATC TTGTTTACAG CGCTTCAGTA CAATGCCGTT CCTGTACTGC 
AACCCAGGGG ATGTCTGTTA TTATGCCAAC CGTAACGATA AGTCTTATTG GCTTTCCACC 
ACTGCACCTC TTCCAATGAT GCCTGTGGTT GAAGAAGAAA TTAGGCCATA TATTAGCCGT 
TGCTCAGTGT GTGAGGCTCC AGCAGTAGCA ATTGCTGTTC ACAGTCAAGA TGTCAGTATC 
CCACATTGCC CAGATGGTTG GCGGAGCTTG TGGATTGGCT ACTCCTTTCT CATGCACACT 
GCCGCAGGCG ATGAGGGGGG AGGACAATCT CTCTCCTCCC CTGGAAGTTG TTTGGAAGAC 
TTCAGGGCGA CTCCATTCAT TGAATGCAAT GGTGGAAGAG GGACCTGCCA CTACTTTGCA 
AACAAATACA GTTTCTGGCT TACTACTATT GACGAGCCCT TCCAGAGCTC TCCGCCTGCA 
GATACACTAA AAGCTGGGCT GATTCGAACT CATATCAGCC GATGCCAAGT CTGCATGAAA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a2 ( XM_002933017.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933063.2 Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X2, mRNA.

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CloneID	OXa06182
Clone ID Related Accession (Same CDS sequence)	XM_004911987.4 , XM_004911987.3 , XM_002933017.2 , XM_002933017.3 , XM_031897040.1 , XM_031897041.1
Accession Version	XM_002933017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-2(IV) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA CTCAAAGCAC CAAGAGATGT CCCACAAGCA GGAGAGAGTA CAGCTGTTTT 
CAGCTCCAAC ACTATTCAGT CACTTGGTGC ATGCTGTTTG TTGTACTGGT GACATGCTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGCC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGAT GCCAGTGTCT GCCCGAGAAA GGAAGTCGTG GACAACCAGG ACCCCTGGGA 
GGGCAAGGTA CCTCTGGCCC ACCTGGGCTA ATAGGAATAG CAGGTCTGCA GGGACGTAAG 
GGAGACAAAG GAGAACGAGG ATTTCCTGGA GTGACTGGCC CCTCTGGAGA TAAGGGCCAA 
AGTGGTGTGA CAGGTTTTCC CGGAGCTGAT GGCGTACCTG GACATACAGG TCAGGGTGGC 
CCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAC AAGGCGATGC TGGTGTGGGA 
GGTTCACATG GTTCAAGAGG CCCTCCTGGA ATTTCTGGTG GATTTGGTGC CAAAGGTCAA 
AAAGGAGAAC CGTTTCATGT ATCAATCGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAAGCAGGCT TTAATGGTAT TCAGGGTCCA AGGGGTTCTC CTGGAACAGC AGGCTTCATT 
GGGCCACAGG GTTACCGTGG ACCTCGTGGG CCACCTGGAC CTCCTGGGCC ACAGGGACCA 
CAAGGCAACA GGGGTCTTGG ATATTATGGA GAAAAAGGAG AACCTGGTGA TCCTGGTCCA 
CCTGGTGAAC AACCTCGTCA AGGAGAGAGA GAACAAAAGC ACAAAATTGA AATTCTCTTG 
CTACAATATA AGGGGGCCAA GGGGGACCAA GGAGAGAAGG GAAATCCTGG CGATAAAGGT 
CAACCAGCTG TCACAGGAGA AAAAGGAGAG GAAGGTATTA CAGGATTTCC TGGACAAAGG 
GGGTTTCCTG GTAATGATGG TAACAGTGGA GCTTCAGGAG AAATAGGGTT TCCTGGGGCA 
AACGGTGTAC CTGGATTACC TGGTACTAAA GGAAAAAAGG GAGAGGTTGG AGACCTAGGA 
CCTCAAGGAC CTGTTACTTT TGTCAATGGT CAACAAAAAA GAAAAGGCTA TGAAGGTGAA 
ATTGGATTTC CAGGACTTAA TGGGGTTTAT GGAGATGATG GAGACCCTGG AGATCCTGGT 
TTTCCAGGCC AGAATGGATC ACCAAGTAGA TTTCCAGGAC AAACAGGTGA TACAGGCTTG 
AAAGGTCAAA GGGGTCCCAA AGGTTACCAA GGTGATACAG GCTTACCAGC ATTTCAGCCT 
GGACCCCCAG GTATAGATGG AGCCCCTGGC AAAGAAGGAC CACCTGGCCA ACCAGGTTAT 
CCAGGTGGAC GCATTGGGAT GAAGGGATTA CCCGGGTTTC CTGGAAGGGA TGGACAAAGA 
GGAGCTCCTG GTTCTCGAGG TTTAAGGGGA ATCAAAGGTG AACAAGGGGA ATGCAGATGC 
TATGAAGGAG ATGAAGCTGG CAGAGGCCCT GCTGGAGAGC CTGGTCCAGT TGGTTTTCCA 
GGTTTTCAGG GTCACGCAGG CAGAAAGGGA GAAGCAGGAG ATAGAGGAGT ACAAGGGCTT 
CCAGGAGCAC CAGGCACAGT GGGTTTAGCT GGTCCTGCTG GGTTTCCTGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTATT TGCTACAGAA AAAGGTTCCA AGGGAGCACA AGGAGACTCT 
GGTTTTCCTG GTGTTCCTGG GCGTGATGGA TACCCTGGAA GAAATGGTAG AGATGGTAGT 
CCTGGCTTTC CAGGCCCTTC TGGAGATGGT ATAAAAGGAC TCCCAGGTTC TCATGGGTTT 
CCAGGACTCC CTGGTAATGC TGGTGCTCGA GGTGAGACGG GCTTTCCTGG ACCAGGATTT 
CCAGGTTCAA AAGGGCTGCG GGGAGAGCCA GGAAACAGAG GAGTGAGCGG ATTTCCAGGA 
GTTCCTGGAC TTTCAGGCCC CCAAGGTGAC TGCTATCAAG AAACTGATGA AGAAAATGAA 
ATAGGGAAAG AAGGGACTGG CTGCAGACTA ATTGCACCTC CTGCTCCAGC AGGTGCCCCA 
GGGCCACCAG GATTACCAGG ATATAACGGT ATAAGAGGCC AGAAAGGTTT TCCCGGACCA 
CATGGTTTTG ATGGCGTTTT TGGATTAAAA GGCATCAAAG GAGACCGTGG AACCGATAGT 
TTGCCAGGTC CACCAGGTTT TTCTGGACAA AGGGGAGACA GAGGTAATGC AGGACTACCT 
GGCCTACCCG GGGTACCAGG TTTGTCAGGA AAACCTGGCT TTAAAGGGGT ACAAGGAGAA 
AAAGGAAGTG TAGGTGATCG CTTAGGAGCT CCATCAGGGG AGCAAGGAAA TACTGGCCTG 
CCTGGCTTGC AAGGACTTAA AGGAACACAA GGGGATCAAG GCATACCTGG CCTACAAGGT 
AAAGTTGGAA CACCAGGAAC ACCTGGAAAC AAGGGTATTA AAGGCTCTCC CGGGGCCCCA 
GGAGTACCTG GAATACCAGG ACCTGCAGCA TTATTTGGAT TTCCAGGAAT GCCCGGAAAC 
CCAGGCACAC CAGGACCCCA AGGTTCAATG GGACCTCCAG GTCCTCCAGG ACAGAGAGGA 
GATGAAGGCG AGAAAGGGCC ACCAGGTCCA GCTGGGATGA AAGGGTTACC TGGAGAACTT 
GGTGTTGGAG GATTTATTGG TGTTAGGGGC AGACATGGAA ATCCAGGCAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGCATCAAAG GTTCCCCTGG CATGGAAGGC 
TTCCAGGGAA TGAAAGGAGC GAAGGGGAGA CCTGCATGGA GAGGACTCAA AGGACCAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG GGAAATGGAA CTTCCGGTAC AAAAGGTGAC 
CGTGGGGAAC AAGGACCACC AGGAGACCCC CCCAAAATGC CAGAAATGAT GCTTTTGACA 
AAAGGCGAAA CAGGAGATCA AGGTGTCTCG GGCTTTAAAG GAATTTCTGG ACTTAAAGGC 
AGTAAAGGTA TGCCTGGACC TCCAGGACAG CTAGGCTTAC CTGGCTTGCC TGGACTACGA 
AGCTTTGAAT TTGGAGATAA AGGAGAAACT GGTACTCCTG GGGTTATCGG TAATCAAGGA 
ATTCTAGGAG AAGTTGGTCC AGACGGAATT ATTGGCTTCC CAGGATTTAC TGGTCCCAGA 
GGTACTCCTG GTATTTCTGG CTTTCCTGGT GTTCCTGGTG AAAGAGGCAA ATATGGAGAT 
ATAGGAGAAA GAGGTGATAC TATTGATTTA CCAGGAGAAA GAGGAACAAA GGGTCAGGGT 
GGAACCCCCG GGCTGCCAGG ACAGCGAGGA ATACACGGAG AAAAAGGAAC TACAGGAGAT 
GAAGGTTTTC GGGGTATTGA GGGAGTGATA GGGGAACACG GACAAACAGG AGAAAAAGGA 
TTCCCAGGTC AACAAGGCCT TGTGGGCTTT CCAGGCTCAC AGGGTTTTCC AGGTCTGCCA 
GGTGTGCCTG GCGAGAAAGG AAGTTCTGGG TTTCCTGGGT TACCTGGTCA ACATGGTTTT 
CCTGGCATAA GAGGGATTGC TGGCCTAGAT GGTCTGCCAG GCACTAAAGG AATTAATGGA 
CAGCCAGGTG CGGATATAAT TGGTTTAAAG GGATTTAAAG GTCTAAGTGG TGGAAAGGGT 
CAACCAGGAG AGGCTAGTAC CATTGTAGGA ATGCCTGGAC CTGCAGGATC TAAAGGAGCC 
GAAGGGGATC AAGGTTTACC TGGCTTAATT GGACTTCCTG GAACAGTTGG AGTCCGTGGC 
TTGCCTGGGT TTTCTAACAA GACAGGTTTG CTAGGAGATG TTGGACCTCC AGGACCTCCT 
GGACTGCCAG GTTTTCCAGG CCCTATCGGA AGACCAGGTT TACCCTCTAA ACCTGGAGTA 
AAAGGAGTCA TAGGGGATCT GGGGTTACAA GGGAACTATG GTGCTAAAGG TATTCCTGGT 
GACGAAGGAA AAGTGGGAGT TCCTGGACTG GCTGGAATAC CTGGAGCCAA AGGTTTGAAA 
GGCAGTGCTG GCTTCCAGGG TTTTGTTGGG GTATCAGGGT ATCGAGGAGA TCAAGGACCT 
AAAGGGCTTA GAGGTGACAT CGGAGAATAT GGTTTAAAGG GTCCTCCTGG ACAACCGGGC 
CCTATGTCTG AACCTCCTCT TATTACGGCT GAACAAGGCT ACCCTGGAGC ACACGGAATT 
ATTGGCCATC AAGGTGTTCA GGGCGATATG GGCCCAAAAG GTCTTGTGGG TACTCCAGGT 
GCCCCTGGCC AAACCGGTAA AGAAGGCTTA CCAGGTGTAC CAGGCTGGCC TGCTCCTCCA 
GGCAGTCGTG GAGAACAAGG ACCCATGGGA CGAACAGGAC CTAATGGATT CAAAGGTGCC 
AGTGGTGTGC CAGGCAGGGC AGGGCTTCCT GGGATGCCCG GGAGAAGTGT CAATATTGGC 
TATCTTTTAG TCAAGCACAG TCAAACAGAT GAAGAGCCAA TGTGTCCTGT TGGTATGGCC 
CGCTTGTGGA CTGGGTATAG TCTCTTGTAT TTTGAAGGAC AGGAAAAAGC ACACAATCAA 
GACTTAGGTC TAGCTGGATC TTGTTTACAG CGCTTCAGTA CAATGCCGTT CCTGTACTGC 
AACCCAGGGG ATGTCTGTTA TTATGCCAAC CGTAACGATA AGTCTTATTG GCTTTCCACC 
ACTGCACCTC TTCCAATGAT GCCTGTGGTT GAAGAAGAAA TTAGGCCATA TATTAGCCGT 
TGCTCAGTGT GTGAGGCTCC AGCAGTAGCA ATTGCTGTTC ACAGTCAAGA TGTCAGTATC 
CCACATTGCC CAGATGGTTG GCGGAGCTTG TGGATTGGCT ACTCCTTTCT CATGCACACT 
GCCGCAGGCG ATGAGGGGGG AGGACAATCT CTCTCCTCCC CTGGAAGTTG TTTGGAAGAC 
TTCAGGGCGA CTCCATTCAT TGAATGCAAT GGTGGAAGAG GGACCTGCCA CTACTTTGCA 
AACAAATACA GTTTCTGGCT TACTACTATT GACGAGCCCT TCCAGAGCTC TCCGCCTGCA 
GATACACTAA AAGCTGGGCT GATTCGAACT CATATCAGCC GATGCCAAGT CTGCATGAAA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933063.2
CDS	126..5294
Translation	MAGTQSTKRCPTSRREYSCFQLQHYSVTWCMLFVVLVTCFAGNAHAGDKSYTGPCGGRDCSQGCQCLPEKGSRGQPGPLGGQGTSGPPGLIGIAGLQGRKGDKGERGFPGVTGPSGDKGQSGVTGFPGADGVPGHTGQGGPRGKPGHDGCNGTQGDAGVGGSHGSRGPPGISGGFGAKGQKGEPFHVSIEVKNRLRGDPGEAGFNGIQGPRGSPGTAGFIGPQGYRGPRGPPGPPGPQGPQGNRGLGYYGEKGEPGDPGPPGEQPRQGEREQKHKIEILLLQYKGAKGDQGEKGNPGDKGQPAVTGEKGEEGITGFPGQRGFPGNDGNSGASGEIGFPGANGVPGLPGTKGKKGEVGDLGPQGPVTFVNGQQKRKGYEGEIGFPGLNGVYGDDGDPGDPGFPGQNGSPSRFPGQTGDTGLKGQRGPKGYQGDTGLPAFQPGPPGIDGAPGKEGPPGQPGYPGGRIGMKGLPGFPGRDGQRGAPGSRGLRGIKGEQGECRCYEGDEAGRGPAGEPGPVGFPGFQGHAGRKGEAGDRGVQGLPGAPGTVGLAGPAGFPGPAGEKGDKVFATEKGSKGAQGDSGFPGVPGRDGYPGRNGRDGSPGFPGPSGDGIKGLPGSHGFPGLPGNAGARGETGFPGPGFPGSKGLRGEPGNRGVSGFPGVPGLSGPQGDCYQETDEENEIGKEGTGCRLIAPPAPAGAPGPPGLPGYNGIRGQKGFPGPHGFDGVFGLKGIKGDRGTDSLPGPPGFSGQRGDRGNAGLPGLPGVPGLSGKPGFKGVQGEKGSVGDRLGAPSGEQGNTGLPGLQGLKGTQGDQGIPGLQGKVGTPGTPGNKGIKGSPGAPGVPGIPGPAALFGFPGMPGNPGTPGPQGSMGPPGPPGQRGDEGEKGPPGPAGMKGLPGELGVGGFIGVRGRHGNPGIPGLVGMHGLPGVKGIKGSPGMEGFQGMKGAKGRPAWRGLKGPSGNIGFPGVKGGNGTSGTKGDRGEQGPPGDPPKMPEMMLLTKGETGDQGVSGFKGISGLKGSKGMPGPPGQLGLPGLPGLRSFEFGDKGETGTPGVIGNQGILGEVGPDGIIGFPGFTGPRGTPGISGFPGVPGERGKYGDIGERGDTIDLPGERGTKGQGGTPGLPGQRGIHGEKGTTGDEGFRGIEGVIGEHGQTGEKGFPGQQGLVGFPGSQGFPGLPGVPGEKGSSGFPGLPGQHGFPGIRGIAGLDGLPGTKGINGQPGADIIGLKGFKGLSGGKGQPGEASTIVGMPGPAGSKGAEGDQGLPGLIGLPGTVGVRGLPGFSNKTGLLGDVGPPGPPGLPGFPGPIGRPGLPSKPGVKGVIGDLGLQGNYGAKGIPGDEGKVGVPGLAGIPGAKGLKGSAGFQGFVGVSGYRGDQGPKGLRGDIGEYGLKGPPGQPGPMSEPPLITAEQGYPGAHGIIGHQGVQGDMGPKGLVGTPGAPGQTGKEGLPGVPGWPAPPGSRGEQGPMGRTGPNGFKGASGVPGRAGLPGMPGRSVNIGYLLVKHSQTDEEPMCPVGMARLWTGYSLLYFEGQEKAHNQDLGLAGSCLQRFSTMPFLYCNPGDVCYYANRNDKSYWLSTTAPLPMMPVVEEEIRPYISRCSVCEAPAVAIAVHSQDVSIPHCPDGWRSLWIGYSFLMHTAAGDEGGGQSLSSPGSCLEDFRATPFIECNGGRGTCHYFANKYSFWLTTIDEPFQSSPPADTLKAGLIRTHISRCQVCMKNL

Target ORF information:

RefSeq Version	XM_002933017.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933017.3

ORF Insert Sequence:

ATGGCAGGAA CTCAAAGCAC CAAGAGATGT CCCACAAGCA GGAGAGAGTA CAGCTGTTTT 
CAGCTCCAAC ACTATTCAGT CACTTGGTGC ATGCTGTTTG TTGTACTGGT GACATGCTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGCC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGAT GCCAGTGTCT GCCCGAGAAA GGAAGTCGTG GACAACCAGG ACCCCTGGGA 
GGGCAAGGTA CCTCTGGCCC ACCTGGGCTA ATAGGAATAG CAGGTCTGCA GGGACGTAAG 
GGAGACAAAG GAGAACGAGG ATTTCCTGGA GTGACTGGCC CCTCTGGAGA TAAGGGCCAA 
AGTGGTGTGA CAGGTTTTCC CGGAGCTGAT GGCGTACCTG GACATACAGG TCAGGGTGGC 
CCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAC AAGGCGATGC TGGTGTGGGA 
GGTTCACATG GTTCAAGAGG CCCTCCTGGA ATTTCTGGTG GATTTGGTGC CAAAGGTCAA 
AAAGGAGAAC CGTTTCATGT ATCAATCGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAAGCAGGCT TTAATGGTAT TCAGGGTCCA AGGGGTTCTC CTGGAACAGC AGGCTTCATT 
GGGCCACAGG GTTACCGTGG ACCTCGTGGG CCACCTGGAC CTCCTGGGCC ACAGGGACCA 
CAAGGCAACA GGGGTCTTGG ATATTATGGA GAAAAAGGAG AACCTGGTGA TCCTGGTCCA 
CCTGGTGAAC AACCTCGTCA AGGAGAGAGA GAACAAAAGC ACAAAATTGA AATTCTCTTG 
CTACAATATA AGGGGGCCAA GGGGGACCAA GGAGAGAAGG GAAATCCTGG CGATAAAGGT 
CAACCAGCTG TCACAGGAGA AAAAGGAGAG GAAGGTATTA CAGGATTTCC TGGACAAAGG 
GGGTTTCCTG GTAATGATGG TAACAGTGGA GCTTCAGGAG AAATAGGGTT TCCTGGGGCA 
AACGGTGTAC CTGGATTACC TGGTACTAAA GGAAAAAAGG GAGAGGTTGG AGACCTAGGA 
CCTCAAGGAC CTGTTACTTT TGTCAATGGT CAACAAAAAA GAAAAGGCTA TGAAGGTGAA 
ATTGGATTTC CAGGACTTAA TGGGGTTTAT GGAGATGATG GAGACCCTGG AGATCCTGGT 
TTTCCAGGCC AGAATGGATC ACCAAGTAGA TTTCCAGGAC AAACAGGTGA TACAGGCTTG 
AAAGGTCAAA GGGGTCCCAA AGGTTACCAA GGTGATACAG GCTTACCAGC ATTTCAGCCT 
GGACCCCCAG GTATAGATGG AGCCCCTGGC AAAGAAGGAC CACCTGGCCA ACCAGGTTAT 
CCAGGTGGAC GCATTGGGAT GAAGGGATTA CCCGGGTTTC CTGGAAGGGA TGGACAAAGA 
GGAGCTCCTG GTTCTCGAGG TTTAAGGGGA ATCAAAGGTG AACAAGGGGA ATGCAGATGC 
TATGAAGGAG ATGAAGCTGG CAGAGGCCCT GCTGGAGAGC CTGGTCCAGT TGGTTTTCCA 
GGTTTTCAGG GTCACGCAGG CAGAAAGGGA GAAGCAGGAG ATAGAGGAGT ACAAGGGCTT 
CCAGGAGCAC CAGGCACAGT GGGTTTAGCT GGTCCTGCTG GGTTTCCTGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTATT TGCTACAGAA AAAGGTTCCA AGGGAGCACA AGGAGACTCT 
GGTTTTCCTG GTGTTCCTGG GCGTGATGGA TACCCTGGAA GAAATGGTAG AGATGGTAGT 
CCTGGCTTTC CAGGCCCTTC TGGAGATGGT ATAAAAGGAC TCCCAGGTTC TCATGGGTTT 
CCAGGACTCC CTGGTAATGC TGGTGCTCGA GGTGAGACGG GCTTTCCTGG ACCAGGATTT 
CCAGGTTCAA AAGGGCTGCG GGGAGAGCCA GGAAACAGAG GAGTGAGCGG ATTTCCAGGA 
GTTCCTGGAC TTTCAGGCCC CCAAGGTGAC TGCTATCAAG AAACTGATGA AGAAAATGAA 
ATAGGGAAAG AAGGGACTGG CTGCAGACTA ATTGCACCTC CTGCTCCAGC AGGTGCCCCA 
GGGCCACCAG GATTACCAGG ATATAACGGT ATAAGAGGCC AGAAAGGTTT TCCCGGACCA 
CATGGTTTTG ATGGCGTTTT TGGATTAAAA GGCATCAAAG GAGACCGTGG AACCGATAGT 
TTGCCAGGTC CACCAGGTTT TTCTGGACAA AGGGGAGACA GAGGTAATGC AGGACTACCT 
GGCCTACCCG GGGTACCAGG TTTGTCAGGA AAACCTGGCT TTAAAGGGGT ACAAGGAGAA 
AAAGGAAGTG TAGGTGATCG CTTAGGAGCT CCATCAGGGG AGCAAGGAAA TACTGGCCTG 
CCTGGCTTGC AAGGACTTAA AGGAACACAA GGGGATCAAG GCATACCTGG CCTACAAGGT 
AAAGTTGGAA CACCAGGAAC ACCTGGAAAC AAGGGTATTA AAGGCTCTCC CGGGGCCCCA 
GGAGTACCTG GAATACCAGG ACCTGCAGCA TTATTTGGAT TTCCAGGAAT GCCCGGAAAC 
CCAGGCACAC CAGGACCCCA AGGTTCAATG GGACCTCCAG GTCCTCCAGG ACAGAGAGGA 
GATGAAGGCG AGAAAGGGCC ACCAGGTCCA GCTGGGATGA AAGGGTTACC TGGAGAACTT 
GGTGTTGGAG GATTTATTGG TGTTAGGGGC AGACATGGAA ATCCAGGCAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGCATCAAAG GTTCCCCTGG CATGGAAGGC 
TTCCAGGGAA TGAAAGGAGC GAAGGGGAGA CCTGCATGGA GAGGACTCAA AGGACCAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG GGAAATGGAA CTTCCGGTAC AAAAGGTGAC 
CGTGGGGAAC AAGGACCACC AGGAGACCCC CCCAAAATGC CAGAAATGAT GCTTTTGACA 
AAAGGCGAAA CAGGAGATCA AGGTGTCTCG GGCTTTAAAG GAATTTCTGG ACTTAAAGGC 
AGTAAAGGTA TGCCTGGACC TCCAGGACAG CTAGGCTTAC CTGGCTTGCC TGGACTACGA 
AGCTTTGAAT TTGGAGATAA AGGAGAAACT GGTACTCCTG GGGTTATCGG TAATCAAGGA 
ATTCTAGGAG AAGTTGGTCC AGACGGAATT ATTGGCTTCC CAGGATTTAC TGGTCCCAGA 
GGTACTCCTG GTATTTCTGG CTTTCCTGGT GTTCCTGGTG AAAGAGGCAA ATATGGAGAT 
ATAGGAGAAA GAGGTGATAC TATTGATTTA CCAGGAGAAA GAGGAACAAA GGGTCAGGGT 
GGAACCCCCG GGCTGCCAGG ACAGCGAGGA ATACACGGAG AAAAAGGAAC TACAGGAGAT 
GAAGGTTTTC GGGGTATTGA GGGAGTGATA GGGGAACACG GACAAACAGG AGAAAAAGGA 
TTCCCAGGTC AACAAGGCCT TGTGGGCTTT CCAGGCTCAC AGGGTTTTCC AGGTCTGCCA 
GGTGTGCCTG GCGAGAAAGG AAGTTCTGGG TTTCCTGGGT TACCTGGTCA ACATGGTTTT 
CCTGGCATAA GAGGGATTGC TGGCCTAGAT GGTCTGCCAG GCACTAAAGG AATTAATGGA 
CAGCCAGGTG CGGATATAAT TGGTTTAAAG GGATTTAAAG GTCTAAGTGG TGGAAAGGGT 
CAACCAGGAG AGGCTAGTAC CATTGTAGGA ATGCCTGGAC CTGCAGGATC TAAAGGAGCC 
GAAGGGGATC AAGGTTTACC TGGCTTAATT GGACTTCCTG GAACAGTTGG AGTCCGTGGC 
TTGCCTGGGT TTTCTAACAA GACAGGTTTG CTAGGAGATG TTGGACCTCC AGGACCTCCT 
GGACTGCCAG GTTTTCCAGG CCCTATCGGA AGACCAGGTT TACCCTCTAA ACCTGGAGTA 
AAAGGAGTCA TAGGGGATCT GGGGTTACAA GGGAACTATG GTGCTAAAGG TATTCCTGGT 
GACGAAGGAA AAGTGGGAGT TCCTGGACTG GCTGGAATAC CTGGAGCCAA AGGTTTGAAA 
GGCAGTGCTG GCTTCCAGGG TTTTGTTGGG GTATCAGGGT ATCGAGGAGA TCAAGGACCT 
AAAGGGCTTA GAGGTGACAT CGGAGAATAT GGTTTAAAGG GTCCTCCTGG ACAACCGGGC 
CCTATGTCTG AACCTCCTCT TATTACGGCT GAACAAGGCT ACCCTGGAGC ACACGGAATT 
ATTGGCCATC AAGGTGTTCA GGGCGATATG GGCCCAAAAG GTCTTGTGGG TACTCCAGGT 
GCCCCTGGCC AAACCGGTAA AGAAGGCTTA CCAGGTGTAC CAGGCTGGCC TGCTCCTCCA 
GGCAGTCGTG GAGAACAAGG ACCCATGGGA CGAACAGGAC CTAATGGATT CAAAGGTGCC 
AGTGGTGTGC CAGGCAGGGC AGGGCTTCCT GGGATGCCCG GGAGAAGTGT CAATATTGGC 
TATCTTTTAG TCAAGCACAG TCAAACAGAT GAAGAGCCAA TGTGTCCTGT TGGTATGGCC 
CGCTTGTGGA CTGGGTATAG TCTCTTGTAT TTTGAAGGAC AGGAAAAAGC ACACAATCAA 
GACTTAGGTC TAGCTGGATC TTGTTTACAG CGCTTCAGTA CAATGCCGTT CCTGTACTGC 
AACCCAGGGG ATGTCTGTTA TTATGCCAAC CGTAACGATA AGTCTTATTG GCTTTCCACC 
ACTGCACCTC TTCCAATGAT GCCTGTGGTT GAAGAAGAAA TTAGGCCATA TATTAGCCGT 
TGCTCAGTGT GTGAGGCTCC AGCAGTAGCA ATTGCTGTTC ACAGTCAAGA TGTCAGTATC 
CCACATTGCC CAGATGGTTG GCGGAGCTTG TGGATTGGCT ACTCCTTTCT CATGCACACT 
GCCGCAGGCG ATGAGGGGGG AGGACAATCT CTCTCCTCCC CTGGAAGTTG TTTGGAAGAC 
TTCAGGGCGA CTCCATTCAT TGAATGCAAT GGTGGAAGAG GGACCTGCCA CTACTTTGCA 
AACAAATACA GTTTCTGGCT TACTACTATT GACGAGCCCT TCCAGAGCTC TCCGCCTGCA 
GATACACTAA AAGCTGGGCT GATTCGAACT CATATCAGCC GATGCCAAGT CTGCATGAAA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a2 ( XM_031897040.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752900.1 Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa06182
Clone ID Related Accession (Same CDS sequence)	XM_004911987.4 , XM_004911987.3 , XM_002933017.2 , XM_002933017.3 , XM_031897040.1 , XM_031897041.1
Accession Version	XM_031897040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-2(IV) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA CTCAAAGCAC CAAGAGATGT CCCACAAGCA GGAGAGAGTA CAGCTGTTTT 
CAGCTCCAAC ACTATTCAGT CACTTGGTGC ATGCTGTTTG TTGTACTGGT GACATGCTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGCC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGAT GCCAGTGTCT GCCCGAGAAA GGAAGTCGTG GACAACCAGG ACCCCTGGGA 
GGGCAAGGTA CCTCTGGCCC ACCTGGGCTA ATAGGAATAG CAGGTCTGCA GGGACGTAAG 
GGAGACAAAG GAGAACGAGG ATTTCCTGGA GTGACTGGCC CCTCTGGAGA TAAGGGCCAA 
AGTGGTGTGA CAGGTTTTCC CGGAGCTGAT GGCGTACCTG GACATACAGG TCAGGGTGGC 
CCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAC AAGGCGATGC TGGTGTGGGA 
GGTTCACATG GTTCAAGAGG CCCTCCTGGA ATTTCTGGTG GATTTGGTGC CAAAGGTCAA 
AAAGGAGAAC CGTTTCATGT ATCAATCGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAAGCAGGCT TTAATGGTAT TCAGGGTCCA AGGGGTTCTC CTGGAACAGC AGGCTTCATT 
GGGCCACAGG GTTACCGTGG ACCTCGTGGG CCACCTGGAC CTCCTGGGCC ACAGGGACCA 
CAAGGCAACA GGGGTCTTGG ATATTATGGA GAAAAAGGAG AACCTGGTGA TCCTGGTCCA 
CCTGGTGAAC AACCTCGTCA AGGAGAGAGA GAACAAAAGC ACAAAATTGA AATTCTCTTG 
CTACAATATA AGGGGGCCAA GGGGGACCAA GGAGAGAAGG GAAATCCTGG CGATAAAGGT 
CAACCAGCTG TCACAGGAGA AAAAGGAGAG GAAGGTATTA CAGGATTTCC TGGACAAAGG 
GGGTTTCCTG GTAATGATGG TAACAGTGGA GCTTCAGGAG AAATAGGGTT TCCTGGGGCA 
AACGGTGTAC CTGGATTACC TGGTACTAAA GGAAAAAAGG GAGAGGTTGG AGACCTAGGA 
CCTCAAGGAC CTGTTACTTT TGTCAATGGT CAACAAAAAA GAAAAGGCTA TGAAGGTGAA 
ATTGGATTTC CAGGACTTAA TGGGGTTTAT GGAGATGATG GAGACCCTGG AGATCCTGGT 
TTTCCAGGCC AGAATGGATC ACCAAGTAGA TTTCCAGGAC AAACAGGTGA TACAGGCTTG 
AAAGGTCAAA GGGGTCCCAA AGGTTACCAA GGTGATACAG GCTTACCAGC ATTTCAGCCT 
GGACCCCCAG GTATAGATGG AGCCCCTGGC AAAGAAGGAC CACCTGGCCA ACCAGGTTAT 
CCAGGTGGAC GCATTGGGAT GAAGGGATTA CCCGGGTTTC CTGGAAGGGA TGGACAAAGA 
GGAGCTCCTG GTTCTCGAGG TTTAAGGGGA ATCAAAGGTG AACAAGGGGA ATGCAGATGC 
TATGAAGGAG ATGAAGCTGG CAGAGGCCCT GCTGGAGAGC CTGGTCCAGT TGGTTTTCCA 
GGTTTTCAGG GTCACGCAGG CAGAAAGGGA GAAGCAGGAG ATAGAGGAGT ACAAGGGCTT 
CCAGGAGCAC CAGGCACAGT GGGTTTAGCT GGTCCTGCTG GGTTTCCTGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTATT TGCTACAGAA AAAGGTTCCA AGGGAGCACA AGGAGACTCT 
GGTTTTCCTG GTGTTCCTGG GCGTGATGGA TACCCTGGAA GAAATGGTAG AGATGGTAGT 
CCTGGCTTTC CAGGCCCTTC TGGAGATGGT ATAAAAGGAC TCCCAGGTTC TCATGGGTTT 
CCAGGACTCC CTGGTAATGC TGGTGCTCGA GGTGAGACGG GCTTTCCTGG ACCAGGATTT 
CCAGGTTCAA AAGGGCTGCG GGGAGAGCCA GGAAACAGAG GAGTGAGCGG ATTTCCAGGA 
GTTCCTGGAC TTTCAGGCCC CCAAGGTGAC TGCTATCAAG AAACTGATGA AGAAAATGAA 
ATAGGGAAAG AAGGGACTGG CTGCAGACTA ATTGCACCTC CTGCTCCAGC AGGTGCCCCA 
GGGCCACCAG GATTACCAGG ATATAACGGT ATAAGAGGCC AGAAAGGTTT TCCCGGACCA 
CATGGTTTTG ATGGCGTTTT TGGATTAAAA GGCATCAAAG GAGACCGTGG AACCGATAGT 
TTGCCAGGTC CACCAGGTTT TTCTGGACAA AGGGGAGACA GAGGTAATGC AGGACTACCT 
GGCCTACCCG GGGTACCAGG TTTGTCAGGA AAACCTGGCT TTAAAGGGGT ACAAGGAGAA 
AAAGGAAGTG TAGGTGATCG CTTAGGAGCT CCATCAGGGG AGCAAGGAAA TACTGGCCTG 
CCTGGCTTGC AAGGACTTAA AGGAACACAA GGGGATCAAG GCATACCTGG CCTACAAGGT 
AAAGTTGGAA CACCAGGAAC ACCTGGAAAC AAGGGTATTA AAGGCTCTCC CGGGGCCCCA 
GGAGTACCTG GAATACCAGG ACCTGCAGCA TTATTTGGAT TTCCAGGAAT GCCCGGAAAC 
CCAGGCACAC CAGGACCCCA AGGTTCAATG GGACCTCCAG GTCCTCCAGG ACAGAGAGGA 
GATGAAGGCG AGAAAGGGCC ACCAGGTCCA GCTGGGATGA AAGGGTTACC TGGAGAACTT 
GGTGTTGGAG GATTTATTGG TGTTAGGGGC AGACATGGAA ATCCAGGCAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGCATCAAAG GTTCCCCTGG CATGGAAGGC 
TTCCAGGGAA TGAAAGGAGC GAAGGGGAGA CCTGCATGGA GAGGACTCAA AGGACCAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG GGAAATGGAA CTTCCGGTAC AAAAGGTGAC 
CGTGGGGAAC AAGGACCACC AGGAGACCCC CCCAAAATGC CAGAAATGAT GCTTTTGACA 
AAAGGCGAAA CAGGAGATCA AGGTGTCTCG GGCTTTAAAG GAATTTCTGG ACTTAAAGGC 
AGTAAAGGTA TGCCTGGACC TCCAGGACAG CTAGGCTTAC CTGGCTTGCC TGGACTACGA 
AGCTTTGAAT TTGGAGATAA AGGAGAAACT GGTACTCCTG GGGTTATCGG TAATCAAGGA 
ATTCTAGGAG AAGTTGGTCC AGACGGAATT ATTGGCTTCC CAGGATTTAC TGGTCCCAGA 
GGTACTCCTG GTATTTCTGG CTTTCCTGGT GTTCCTGGTG AAAGAGGCAA ATATGGAGAT 
ATAGGAGAAA GAGGTGATAC TATTGATTTA CCAGGAGAAA GAGGAACAAA GGGTCAGGGT 
GGAACCCCCG GGCTGCCAGG ACAGCGAGGA ATACACGGAG AAAAAGGAAC TACAGGAGAT 
GAAGGTTTTC GGGGTATTGA GGGAGTGATA GGGGAACACG GACAAACAGG AGAAAAAGGA 
TTCCCAGGTC AACAAGGCCT TGTGGGCTTT CCAGGCTCAC AGGGTTTTCC AGGTCTGCCA 
GGTGTGCCTG GCGAGAAAGG AAGTTCTGGG TTTCCTGGGT TACCTGGTCA ACATGGTTTT 
CCTGGCATAA GAGGGATTGC TGGCCTAGAT GGTCTGCCAG GCACTAAAGG AATTAATGGA 
CAGCCAGGTG CGGATATAAT TGGTTTAAAG GGATTTAAAG GTCTAAGTGG TGGAAAGGGT 
CAACCAGGAG AGGCTAGTAC CATTGTAGGA ATGCCTGGAC CTGCAGGATC TAAAGGAGCC 
GAAGGGGATC AAGGTTTACC TGGCTTAATT GGACTTCCTG GAACAGTTGG AGTCCGTGGC 
TTGCCTGGGT TTTCTAACAA GACAGGTTTG CTAGGAGATG TTGGACCTCC AGGACCTCCT 
GGACTGCCAG GTTTTCCAGG CCCTATCGGA AGACCAGGTT TACCCTCTAA ACCTGGAGTA 
AAAGGAGTCA TAGGGGATCT GGGGTTACAA GGGAACTATG GTGCTAAAGG TATTCCTGGT 
GACGAAGGAA AAGTGGGAGT TCCTGGACTG GCTGGAATAC CTGGAGCCAA AGGTTTGAAA 
GGCAGTGCTG GCTTCCAGGG TTTTGTTGGG GTATCAGGGT ATCGAGGAGA TCAAGGACCT 
AAAGGGCTTA GAGGTGACAT CGGAGAATAT GGTTTAAAGG GTCCTCCTGG ACAACCGGGC 
CCTATGTCTG AACCTCCTCT TATTACGGCT GAACAAGGCT ACCCTGGAGC ACACGGAATT 
ATTGGCCATC AAGGTGTTCA GGGCGATATG GGCCCAAAAG GTCTTGTGGG TACTCCAGGT 
GCCCCTGGCC AAACCGGTAA AGAAGGCTTA CCAGGTGTAC CAGGCTGGCC TGCTCCTCCA 
GGCAGTCGTG GAGAACAAGG ACCCATGGGA CGAACAGGAC CTAATGGATT CAAAGGTGCC 
AGTGGTGTGC CAGGCAGGGC AGGGCTTCCT GGGATGCCCG GGAGAAGTGT CAATATTGGC 
TATCTTTTAG TCAAGCACAG TCAAACAGAT GAAGAGCCAA TGTGTCCTGT TGGTATGGCC 
CGCTTGTGGA CTGGGTATAG TCTCTTGTAT TTTGAAGGAC AGGAAAAAGC ACACAATCAA 
GACTTAGGTC TAGCTGGATC TTGTTTACAG CGCTTCAGTA CAATGCCGTT CCTGTACTGC 
AACCCAGGGG ATGTCTGTTA TTATGCCAAC CGTAACGATA AGTCTTATTG GCTTTCCACC 
ACTGCACCTC TTCCAATGAT GCCTGTGGTT GAAGAAGAAA TTAGGCCATA TATTAGCCGT 
TGCTCAGTGT GTGAGGCTCC AGCAGTAGCA ATTGCTGTTC ACAGTCAAGA TGTCAGTATC 
CCACATTGCC CAGATGGTTG GCGGAGCTTG TGGATTGGCT ACTCCTTTCT CATGCACACT 
GCCGCAGGCG ATGAGGGGGG AGGACAATCT CTCTCCTCCC CTGGAAGTTG TTTGGAAGAC 
TTCAGGGCGA CTCCATTCAT TGAATGCAAT GGTGGAAGAG GGACCTGCCA CTACTTTGCA 
AACAAATACA GTTTCTGGCT TACTACTATT GACGAGCCCT TCCAGAGCTC TCCGCCTGCA 
GATACACTAA AAGCTGGGCT GATTCGAACT CATATCAGCC GATGCCAAGT CTGCATGAAA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752900.1
CDS	211..5379
Translation	MAGTQSTKRCPTSRREYSCFQLQHYSVTWCMLFVVLVTCFAGNAHAGDKSYTGPCGGRDCSQGCQCLPEKGSRGQPGPLGGQGTSGPPGLIGIAGLQGRKGDKGERGFPGVTGPSGDKGQSGVTGFPGADGVPGHTGQGGPRGKPGHDGCNGTQGDAGVGGSHGSRGPPGISGGFGAKGQKGEPFHVSIEVKNRLRGDPGEAGFNGIQGPRGSPGTAGFIGPQGYRGPRGPPGPPGPQGPQGNRGLGYYGEKGEPGDPGPPGEQPRQGEREQKHKIEILLLQYKGAKGDQGEKGNPGDKGQPAVTGEKGEEGITGFPGQRGFPGNDGNSGASGEIGFPGANGVPGLPGTKGKKGEVGDLGPQGPVTFVNGQQKRKGYEGEIGFPGLNGVYGDDGDPGDPGFPGQNGSPSRFPGQTGDTGLKGQRGPKGYQGDTGLPAFQPGPPGIDGAPGKEGPPGQPGYPGGRIGMKGLPGFPGRDGQRGAPGSRGLRGIKGEQGECRCYEGDEAGRGPAGEPGPVGFPGFQGHAGRKGEAGDRGVQGLPGAPGTVGLAGPAGFPGPAGEKGDKVFATEKGSKGAQGDSGFPGVPGRDGYPGRNGRDGSPGFPGPSGDGIKGLPGSHGFPGLPGNAGARGETGFPGPGFPGSKGLRGEPGNRGVSGFPGVPGLSGPQGDCYQETDEENEIGKEGTGCRLIAPPAPAGAPGPPGLPGYNGIRGQKGFPGPHGFDGVFGLKGIKGDRGTDSLPGPPGFSGQRGDRGNAGLPGLPGVPGLSGKPGFKGVQGEKGSVGDRLGAPSGEQGNTGLPGLQGLKGTQGDQGIPGLQGKVGTPGTPGNKGIKGSPGAPGVPGIPGPAALFGFPGMPGNPGTPGPQGSMGPPGPPGQRGDEGEKGPPGPAGMKGLPGELGVGGFIGVRGRHGNPGIPGLVGMHGLPGVKGIKGSPGMEGFQGMKGAKGRPAWRGLKGPSGNIGFPGVKGGNGTSGTKGDRGEQGPPGDPPKMPEMMLLTKGETGDQGVSGFKGISGLKGSKGMPGPPGQLGLPGLPGLRSFEFGDKGETGTPGVIGNQGILGEVGPDGIIGFPGFTGPRGTPGISGFPGVPGERGKYGDIGERGDTIDLPGERGTKGQGGTPGLPGQRGIHGEKGTTGDEGFRGIEGVIGEHGQTGEKGFPGQQGLVGFPGSQGFPGLPGVPGEKGSSGFPGLPGQHGFPGIRGIAGLDGLPGTKGINGQPGADIIGLKGFKGLSGGKGQPGEASTIVGMPGPAGSKGAEGDQGLPGLIGLPGTVGVRGLPGFSNKTGLLGDVGPPGPPGLPGFPGPIGRPGLPSKPGVKGVIGDLGLQGNYGAKGIPGDEGKVGVPGLAGIPGAKGLKGSAGFQGFVGVSGYRGDQGPKGLRGDIGEYGLKGPPGQPGPMSEPPLITAEQGYPGAHGIIGHQGVQGDMGPKGLVGTPGAPGQTGKEGLPGVPGWPAPPGSRGEQGPMGRTGPNGFKGASGVPGRAGLPGMPGRSVNIGYLLVKHSQTDEEPMCPVGMARLWTGYSLLYFEGQEKAHNQDLGLAGSCLQRFSTMPFLYCNPGDVCYYANRNDKSYWLSTTAPLPMMPVVEEEIRPYISRCSVCEAPAVAIAVHSQDVSIPHCPDGWRSLWIGYSFLMHTAAGDEGGGQSLSSPGSCLEDFRATPFIECNGGRGTCHYFANKYSFWLTTIDEPFQSSPPADTLKAGLIRTHISRCQVCMKNL

Target ORF information:

RefSeq Version	XM_031897040.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897040.1

ORF Insert Sequence:

ATGGCAGGAA CTCAAAGCAC CAAGAGATGT CCCACAAGCA GGAGAGAGTA CAGCTGTTTT 
CAGCTCCAAC ACTATTCAGT CACTTGGTGC ATGCTGTTTG TTGTACTGGT GACATGCTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGCC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGAT GCCAGTGTCT GCCCGAGAAA GGAAGTCGTG GACAACCAGG ACCCCTGGGA 
GGGCAAGGTA CCTCTGGCCC ACCTGGGCTA ATAGGAATAG CAGGTCTGCA GGGACGTAAG 
GGAGACAAAG GAGAACGAGG ATTTCCTGGA GTGACTGGCC CCTCTGGAGA TAAGGGCCAA 
AGTGGTGTGA CAGGTTTTCC CGGAGCTGAT GGCGTACCTG GACATACAGG TCAGGGTGGC 
CCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAC AAGGCGATGC TGGTGTGGGA 
GGTTCACATG GTTCAAGAGG CCCTCCTGGA ATTTCTGGTG GATTTGGTGC CAAAGGTCAA 
AAAGGAGAAC CGTTTCATGT ATCAATCGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAAGCAGGCT TTAATGGTAT TCAGGGTCCA AGGGGTTCTC CTGGAACAGC AGGCTTCATT 
GGGCCACAGG GTTACCGTGG ACCTCGTGGG CCACCTGGAC CTCCTGGGCC ACAGGGACCA 
CAAGGCAACA GGGGTCTTGG ATATTATGGA GAAAAAGGAG AACCTGGTGA TCCTGGTCCA 
CCTGGTGAAC AACCTCGTCA AGGAGAGAGA GAACAAAAGC ACAAAATTGA AATTCTCTTG 
CTACAATATA AGGGGGCCAA GGGGGACCAA GGAGAGAAGG GAAATCCTGG CGATAAAGGT 
CAACCAGCTG TCACAGGAGA AAAAGGAGAG GAAGGTATTA CAGGATTTCC TGGACAAAGG 
GGGTTTCCTG GTAATGATGG TAACAGTGGA GCTTCAGGAG AAATAGGGTT TCCTGGGGCA 
AACGGTGTAC CTGGATTACC TGGTACTAAA GGAAAAAAGG GAGAGGTTGG AGACCTAGGA 
CCTCAAGGAC CTGTTACTTT TGTCAATGGT CAACAAAAAA GAAAAGGCTA TGAAGGTGAA 
ATTGGATTTC CAGGACTTAA TGGGGTTTAT GGAGATGATG GAGACCCTGG AGATCCTGGT 
TTTCCAGGCC AGAATGGATC ACCAAGTAGA TTTCCAGGAC AAACAGGTGA TACAGGCTTG 
AAAGGTCAAA GGGGTCCCAA AGGTTACCAA GGTGATACAG GCTTACCAGC ATTTCAGCCT 
GGACCCCCAG GTATAGATGG AGCCCCTGGC AAAGAAGGAC CACCTGGCCA ACCAGGTTAT 
CCAGGTGGAC GCATTGGGAT GAAGGGATTA CCCGGGTTTC CTGGAAGGGA TGGACAAAGA 
GGAGCTCCTG GTTCTCGAGG TTTAAGGGGA ATCAAAGGTG AACAAGGGGA ATGCAGATGC 
TATGAAGGAG ATGAAGCTGG CAGAGGCCCT GCTGGAGAGC CTGGTCCAGT TGGTTTTCCA 
GGTTTTCAGG GTCACGCAGG CAGAAAGGGA GAAGCAGGAG ATAGAGGAGT ACAAGGGCTT 
CCAGGAGCAC CAGGCACAGT GGGTTTAGCT GGTCCTGCTG GGTTTCCTGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTATT TGCTACAGAA AAAGGTTCCA AGGGAGCACA AGGAGACTCT 
GGTTTTCCTG GTGTTCCTGG GCGTGATGGA TACCCTGGAA GAAATGGTAG AGATGGTAGT 
CCTGGCTTTC CAGGCCCTTC TGGAGATGGT ATAAAAGGAC TCCCAGGTTC TCATGGGTTT 
CCAGGACTCC CTGGTAATGC TGGTGCTCGA GGTGAGACGG GCTTTCCTGG ACCAGGATTT 
CCAGGTTCAA AAGGGCTGCG GGGAGAGCCA GGAAACAGAG GAGTGAGCGG ATTTCCAGGA 
GTTCCTGGAC TTTCAGGCCC CCAAGGTGAC TGCTATCAAG AAACTGATGA AGAAAATGAA 
ATAGGGAAAG AAGGGACTGG CTGCAGACTA ATTGCACCTC CTGCTCCAGC AGGTGCCCCA 
GGGCCACCAG GATTACCAGG ATATAACGGT ATAAGAGGCC AGAAAGGTTT TCCCGGACCA 
CATGGTTTTG ATGGCGTTTT TGGATTAAAA GGCATCAAAG GAGACCGTGG AACCGATAGT 
TTGCCAGGTC CACCAGGTTT TTCTGGACAA AGGGGAGACA GAGGTAATGC AGGACTACCT 
GGCCTACCCG GGGTACCAGG TTTGTCAGGA AAACCTGGCT TTAAAGGGGT ACAAGGAGAA 
AAAGGAAGTG TAGGTGATCG CTTAGGAGCT CCATCAGGGG AGCAAGGAAA TACTGGCCTG 
CCTGGCTTGC AAGGACTTAA AGGAACACAA GGGGATCAAG GCATACCTGG CCTACAAGGT 
AAAGTTGGAA CACCAGGAAC ACCTGGAAAC AAGGGTATTA AAGGCTCTCC CGGGGCCCCA 
GGAGTACCTG GAATACCAGG ACCTGCAGCA TTATTTGGAT TTCCAGGAAT GCCCGGAAAC 
CCAGGCACAC CAGGACCCCA AGGTTCAATG GGACCTCCAG GTCCTCCAGG ACAGAGAGGA 
GATGAAGGCG AGAAAGGGCC ACCAGGTCCA GCTGGGATGA AAGGGTTACC TGGAGAACTT 
GGTGTTGGAG GATTTATTGG TGTTAGGGGC AGACATGGAA ATCCAGGCAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGCATCAAAG GTTCCCCTGG CATGGAAGGC 
TTCCAGGGAA TGAAAGGAGC GAAGGGGAGA CCTGCATGGA GAGGACTCAA AGGACCAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG GGAAATGGAA CTTCCGGTAC AAAAGGTGAC 
CGTGGGGAAC AAGGACCACC AGGAGACCCC CCCAAAATGC CAGAAATGAT GCTTTTGACA 
AAAGGCGAAA CAGGAGATCA AGGTGTCTCG GGCTTTAAAG GAATTTCTGG ACTTAAAGGC 
AGTAAAGGTA TGCCTGGACC TCCAGGACAG CTAGGCTTAC CTGGCTTGCC TGGACTACGA 
AGCTTTGAAT TTGGAGATAA AGGAGAAACT GGTACTCCTG GGGTTATCGG TAATCAAGGA 
ATTCTAGGAG AAGTTGGTCC AGACGGAATT ATTGGCTTCC CAGGATTTAC TGGTCCCAGA 
GGTACTCCTG GTATTTCTGG CTTTCCTGGT GTTCCTGGTG AAAGAGGCAA ATATGGAGAT 
ATAGGAGAAA GAGGTGATAC TATTGATTTA CCAGGAGAAA GAGGAACAAA GGGTCAGGGT 
GGAACCCCCG GGCTGCCAGG ACAGCGAGGA ATACACGGAG AAAAAGGAAC TACAGGAGAT 
GAAGGTTTTC GGGGTATTGA GGGAGTGATA GGGGAACACG GACAAACAGG AGAAAAAGGA 
TTCCCAGGTC AACAAGGCCT TGTGGGCTTT CCAGGCTCAC AGGGTTTTCC AGGTCTGCCA 
GGTGTGCCTG GCGAGAAAGG AAGTTCTGGG TTTCCTGGGT TACCTGGTCA ACATGGTTTT 
CCTGGCATAA GAGGGATTGC TGGCCTAGAT GGTCTGCCAG GCACTAAAGG AATTAATGGA 
CAGCCAGGTG CGGATATAAT TGGTTTAAAG GGATTTAAAG GTCTAAGTGG TGGAAAGGGT 
CAACCAGGAG AGGCTAGTAC CATTGTAGGA ATGCCTGGAC CTGCAGGATC TAAAGGAGCC 
GAAGGGGATC AAGGTTTACC TGGCTTAATT GGACTTCCTG GAACAGTTGG AGTCCGTGGC 
TTGCCTGGGT TTTCTAACAA GACAGGTTTG CTAGGAGATG TTGGACCTCC AGGACCTCCT 
GGACTGCCAG GTTTTCCAGG CCCTATCGGA AGACCAGGTT TACCCTCTAA ACCTGGAGTA 
AAAGGAGTCA TAGGGGATCT GGGGTTACAA GGGAACTATG GTGCTAAAGG TATTCCTGGT 
GACGAAGGAA AAGTGGGAGT TCCTGGACTG GCTGGAATAC CTGGAGCCAA AGGTTTGAAA 
GGCAGTGCTG GCTTCCAGGG TTTTGTTGGG GTATCAGGGT ATCGAGGAGA TCAAGGACCT 
AAAGGGCTTA GAGGTGACAT CGGAGAATAT GGTTTAAAGG GTCCTCCTGG ACAACCGGGC 
CCTATGTCTG AACCTCCTCT TATTACGGCT GAACAAGGCT ACCCTGGAGC ACACGGAATT 
ATTGGCCATC AAGGTGTTCA GGGCGATATG GGCCCAAAAG GTCTTGTGGG TACTCCAGGT 
GCCCCTGGCC AAACCGGTAA AGAAGGCTTA CCAGGTGTAC CAGGCTGGCC TGCTCCTCCA 
GGCAGTCGTG GAGAACAAGG ACCCATGGGA CGAACAGGAC CTAATGGATT CAAAGGTGCC 
AGTGGTGTGC CAGGCAGGGC AGGGCTTCCT GGGATGCCCG GGAGAAGTGT CAATATTGGC 
TATCTTTTAG TCAAGCACAG TCAAACAGAT GAAGAGCCAA TGTGTCCTGT TGGTATGGCC 
CGCTTGTGGA CTGGGTATAG TCTCTTGTAT TTTGAAGGAC AGGAAAAAGC ACACAATCAA 
GACTTAGGTC TAGCTGGATC TTGTTTACAG CGCTTCAGTA CAATGCCGTT CCTGTACTGC 
AACCCAGGGG ATGTCTGTTA TTATGCCAAC CGTAACGATA AGTCTTATTG GCTTTCCACC 
ACTGCACCTC TTCCAATGAT GCCTGTGGTT GAAGAAGAAA TTAGGCCATA TATTAGCCGT 
TGCTCAGTGT GTGAGGCTCC AGCAGTAGCA ATTGCTGTTC ACAGTCAAGA TGTCAGTATC 
CCACATTGCC CAGATGGTTG GCGGAGCTTG TGGATTGGCT ACTCCTTTCT CATGCACACT 
GCCGCAGGCG ATGAGGGGGG AGGACAATCT CTCTCCTCCC CTGGAAGTTG TTTGGAAGAC 
TTCAGGGCGA CTCCATTCAT TGAATGCAAT GGTGGAAGAG GGACCTGCCA CTACTTTGCA 
AACAAATACA GTTTCTGGCT TACTACTATT GACGAGCCCT TCCAGAGCTC TCCGCCTGCA 
GATACACTAA AAGCTGGGCT GATTCGAACT CATATCAGCC GATGCCAAGT CTGCATGAAA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a2 ( XM_031897041.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752901.1 Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X4, mRNA.

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CloneID	OXa06182
Clone ID Related Accession (Same CDS sequence)	XM_004911987.4 , XM_004911987.3 , XM_002933017.2 , XM_002933017.3 , XM_031897040.1 , XM_031897041.1
Accession Version	XM_031897041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-2(IV) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA CTCAAAGCAC CAAGAGATGT CCCACAAGCA GGAGAGAGTA CAGCTGTTTT 
CAGCTCCAAC ACTATTCAGT CACTTGGTGC ATGCTGTTTG TTGTACTGGT GACATGCTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGCC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGAT GCCAGTGTCT GCCCGAGAAA GGAAGTCGTG GACAACCAGG ACCCCTGGGA 
GGGCAAGGTA CCTCTGGCCC ACCTGGGCTA ATAGGAATAG CAGGTCTGCA GGGACGTAAG 
GGAGACAAAG GAGAACGAGG ATTTCCTGGA GTGACTGGCC CCTCTGGAGA TAAGGGCCAA 
AGTGGTGTGA CAGGTTTTCC CGGAGCTGAT GGCGTACCTG GACATACAGG TCAGGGTGGC 
CCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAC AAGGCGATGC TGGTGTGGGA 
GGTTCACATG GTTCAAGAGG CCCTCCTGGA ATTTCTGGTG GATTTGGTGC CAAAGGTCAA 
AAAGGAGAAC CGTTTCATGT ATCAATCGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAAGCAGGCT TTAATGGTAT TCAGGGTCCA AGGGGTTCTC CTGGAACAGC AGGCTTCATT 
GGGCCACAGG GTTACCGTGG ACCTCGTGGG CCACCTGGAC CTCCTGGGCC ACAGGGACCA 
CAAGGCAACA GGGGTCTTGG ATATTATGGA GAAAAAGGAG AACCTGGTGA TCCTGGTCCA 
CCTGGTGAAC AACCTCGTCA AGGAGAGAGA GAACAAAAGC ACAAAATTGA AATTCTCTTG 
CTACAATATA AGGGGGCCAA GGGGGACCAA GGAGAGAAGG GAAATCCTGG CGATAAAGGT 
CAACCAGCTG TCACAGGAGA AAAAGGAGAG GAAGGTATTA CAGGATTTCC TGGACAAAGG 
GGGTTTCCTG GTAATGATGG TAACAGTGGA GCTTCAGGAG AAATAGGGTT TCCTGGGGCA 
AACGGTGTAC CTGGATTACC TGGTACTAAA GGAAAAAAGG GAGAGGTTGG AGACCTAGGA 
CCTCAAGGAC CTGTTACTTT TGTCAATGGT CAACAAAAAA GAAAAGGCTA TGAAGGTGAA 
ATTGGATTTC CAGGACTTAA TGGGGTTTAT GGAGATGATG GAGACCCTGG AGATCCTGGT 
TTTCCAGGCC AGAATGGATC ACCAAGTAGA TTTCCAGGAC AAACAGGTGA TACAGGCTTG 
AAAGGTCAAA GGGGTCCCAA AGGTTACCAA GGTGATACAG GCTTACCAGC ATTTCAGCCT 
GGACCCCCAG GTATAGATGG AGCCCCTGGC AAAGAAGGAC CACCTGGCCA ACCAGGTTAT 
CCAGGTGGAC GCATTGGGAT GAAGGGATTA CCCGGGTTTC CTGGAAGGGA TGGACAAAGA 
GGAGCTCCTG GTTCTCGAGG TTTAAGGGGA ATCAAAGGTG AACAAGGGGA ATGCAGATGC 
TATGAAGGAG ATGAAGCTGG CAGAGGCCCT GCTGGAGAGC CTGGTCCAGT TGGTTTTCCA 
GGTTTTCAGG GTCACGCAGG CAGAAAGGGA GAAGCAGGAG ATAGAGGAGT ACAAGGGCTT 
CCAGGAGCAC CAGGCACAGT GGGTTTAGCT GGTCCTGCTG GGTTTCCTGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTATT TGCTACAGAA AAAGGTTCCA AGGGAGCACA AGGAGACTCT 
GGTTTTCCTG GTGTTCCTGG GCGTGATGGA TACCCTGGAA GAAATGGTAG AGATGGTAGT 
CCTGGCTTTC CAGGCCCTTC TGGAGATGGT ATAAAAGGAC TCCCAGGTTC TCATGGGTTT 
CCAGGACTCC CTGGTAATGC TGGTGCTCGA GGTGAGACGG GCTTTCCTGG ACCAGGATTT 
CCAGGTTCAA AAGGGCTGCG GGGAGAGCCA GGAAACAGAG GAGTGAGCGG ATTTCCAGGA 
GTTCCTGGAC TTTCAGGCCC CCAAGGTGAC TGCTATCAAG AAACTGATGA AGAAAATGAA 
ATAGGGAAAG AAGGGACTGG CTGCAGACTA ATTGCACCTC CTGCTCCAGC AGGTGCCCCA 
GGGCCACCAG GATTACCAGG ATATAACGGT ATAAGAGGCC AGAAAGGTTT TCCCGGACCA 
CATGGTTTTG ATGGCGTTTT TGGATTAAAA GGCATCAAAG GAGACCGTGG AACCGATAGT 
TTGCCAGGTC CACCAGGTTT TTCTGGACAA AGGGGAGACA GAGGTAATGC AGGACTACCT 
GGCCTACCCG GGGTACCAGG TTTGTCAGGA AAACCTGGCT TTAAAGGGGT ACAAGGAGAA 
AAAGGAAGTG TAGGTGATCG CTTAGGAGCT CCATCAGGGG AGCAAGGAAA TACTGGCCTG 
CCTGGCTTGC AAGGACTTAA AGGAACACAA GGGGATCAAG GCATACCTGG CCTACAAGGT 
AAAGTTGGAA CACCAGGAAC ACCTGGAAAC AAGGGTATTA AAGGCTCTCC CGGGGCCCCA 
GGAGTACCTG GAATACCAGG ACCTGCAGCA TTATTTGGAT TTCCAGGAAT GCCCGGAAAC 
CCAGGCACAC CAGGACCCCA AGGTTCAATG GGACCTCCAG GTCCTCCAGG ACAGAGAGGA 
GATGAAGGCG AGAAAGGGCC ACCAGGTCCA GCTGGGATGA AAGGGTTACC TGGAGAACTT 
GGTGTTGGAG GATTTATTGG TGTTAGGGGC AGACATGGAA ATCCAGGCAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGCATCAAAG GTTCCCCTGG CATGGAAGGC 
TTCCAGGGAA TGAAAGGAGC GAAGGGGAGA CCTGCATGGA GAGGACTCAA AGGACCAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG GGAAATGGAA CTTCCGGTAC AAAAGGTGAC 
CGTGGGGAAC AAGGACCACC AGGAGACCCC CCCAAAATGC CAGAAATGAT GCTTTTGACA 
AAAGGCGAAA CAGGAGATCA AGGTGTCTCG GGCTTTAAAG GAATTTCTGG ACTTAAAGGC 
AGTAAAGGTA TGCCTGGACC TCCAGGACAG CTAGGCTTAC CTGGCTTGCC TGGACTACGA 
AGCTTTGAAT TTGGAGATAA AGGAGAAACT GGTACTCCTG GGGTTATCGG TAATCAAGGA 
ATTCTAGGAG AAGTTGGTCC AGACGGAATT ATTGGCTTCC CAGGATTTAC TGGTCCCAGA 
GGTACTCCTG GTATTTCTGG CTTTCCTGGT GTTCCTGGTG AAAGAGGCAA ATATGGAGAT 
ATAGGAGAAA GAGGTGATAC TATTGATTTA CCAGGAGAAA GAGGAACAAA GGGTCAGGGT 
GGAACCCCCG GGCTGCCAGG ACAGCGAGGA ATACACGGAG AAAAAGGAAC TACAGGAGAT 
GAAGGTTTTC GGGGTATTGA GGGAGTGATA GGGGAACACG GACAAACAGG AGAAAAAGGA 
TTCCCAGGTC AACAAGGCCT TGTGGGCTTT CCAGGCTCAC AGGGTTTTCC AGGTCTGCCA 
GGTGTGCCTG GCGAGAAAGG AAGTTCTGGG TTTCCTGGGT TACCTGGTCA ACATGGTTTT 
CCTGGCATAA GAGGGATTGC TGGCCTAGAT GGTCTGCCAG GCACTAAAGG AATTAATGGA 
CAGCCAGGTG CGGATATAAT TGGTTTAAAG GGATTTAAAG GTCTAAGTGG TGGAAAGGGT 
CAACCAGGAG AGGCTAGTAC CATTGTAGGA ATGCCTGGAC CTGCAGGATC TAAAGGAGCC 
GAAGGGGATC AAGGTTTACC TGGCTTAATT GGACTTCCTG GAACAGTTGG AGTCCGTGGC 
TTGCCTGGGT TTTCTAACAA GACAGGTTTG CTAGGAGATG TTGGACCTCC AGGACCTCCT 
GGACTGCCAG GTTTTCCAGG CCCTATCGGA AGACCAGGTT TACCCTCTAA ACCTGGAGTA 
AAAGGAGTCA TAGGGGATCT GGGGTTACAA GGGAACTATG GTGCTAAAGG TATTCCTGGT 
GACGAAGGAA AAGTGGGAGT TCCTGGACTG GCTGGAATAC CTGGAGCCAA AGGTTTGAAA 
GGCAGTGCTG GCTTCCAGGG TTTTGTTGGG GTATCAGGGT ATCGAGGAGA TCAAGGACCT 
AAAGGGCTTA GAGGTGACAT CGGAGAATAT GGTTTAAAGG GTCCTCCTGG ACAACCGGGC 
CCTATGTCTG AACCTCCTCT TATTACGGCT GAACAAGGCT ACCCTGGAGC ACACGGAATT 
ATTGGCCATC AAGGTGTTCA GGGCGATATG GGCCCAAAAG GTCTTGTGGG TACTCCAGGT 
GCCCCTGGCC AAACCGGTAA AGAAGGCTTA CCAGGTGTAC CAGGCTGGCC TGCTCCTCCA 
GGCAGTCGTG GAGAACAAGG ACCCATGGGA CGAACAGGAC CTAATGGATT CAAAGGTGCC 
AGTGGTGTGC CAGGCAGGGC AGGGCTTCCT GGGATGCCCG GGAGAAGTGT CAATATTGGC 
TATCTTTTAG TCAAGCACAG TCAAACAGAT GAAGAGCCAA TGTGTCCTGT TGGTATGGCC 
CGCTTGTGGA CTGGGTATAG TCTCTTGTAT TTTGAAGGAC AGGAAAAAGC ACACAATCAA 
GACTTAGGTC TAGCTGGATC TTGTTTACAG CGCTTCAGTA CAATGCCGTT CCTGTACTGC 
AACCCAGGGG ATGTCTGTTA TTATGCCAAC CGTAACGATA AGTCTTATTG GCTTTCCACC 
ACTGCACCTC TTCCAATGAT GCCTGTGGTT GAAGAAGAAA TTAGGCCATA TATTAGCCGT 
TGCTCAGTGT GTGAGGCTCC AGCAGTAGCA ATTGCTGTTC ACAGTCAAGA TGTCAGTATC 
CCACATTGCC CAGATGGTTG GCGGAGCTTG TGGATTGGCT ACTCCTTTCT CATGCACACT 
GCCGCAGGCG ATGAGGGGGG AGGACAATCT CTCTCCTCCC CTGGAAGTTG TTTGGAAGAC 
TTCAGGGCGA CTCCATTCAT TGAATGCAAT GGTGGAAGAG GGACCTGCCA CTACTTTGCA 
AACAAATACA GTTTCTGGCT TACTACTATT GACGAGCCCT TCCAGAGCTC TCCGCCTGCA 
GATACACTAA AAGCTGGGCT GATTCGAACT CATATCAGCC GATGCCAAGT CTGCATGAAA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752901.1
CDS	140..5308
Translation	MAGTQSTKRCPTSRREYSCFQLQHYSVTWCMLFVVLVTCFAGNAHAGDKSYTGPCGGRDCSQGCQCLPEKGSRGQPGPLGGQGTSGPPGLIGIAGLQGRKGDKGERGFPGVTGPSGDKGQSGVTGFPGADGVPGHTGQGGPRGKPGHDGCNGTQGDAGVGGSHGSRGPPGISGGFGAKGQKGEPFHVSIEVKNRLRGDPGEAGFNGIQGPRGSPGTAGFIGPQGYRGPRGPPGPPGPQGPQGNRGLGYYGEKGEPGDPGPPGEQPRQGEREQKHKIEILLLQYKGAKGDQGEKGNPGDKGQPAVTGEKGEEGITGFPGQRGFPGNDGNSGASGEIGFPGANGVPGLPGTKGKKGEVGDLGPQGPVTFVNGQQKRKGYEGEIGFPGLNGVYGDDGDPGDPGFPGQNGSPSRFPGQTGDTGLKGQRGPKGYQGDTGLPAFQPGPPGIDGAPGKEGPPGQPGYPGGRIGMKGLPGFPGRDGQRGAPGSRGLRGIKGEQGECRCYEGDEAGRGPAGEPGPVGFPGFQGHAGRKGEAGDRGVQGLPGAPGTVGLAGPAGFPGPAGEKGDKVFATEKGSKGAQGDSGFPGVPGRDGYPGRNGRDGSPGFPGPSGDGIKGLPGSHGFPGLPGNAGARGETGFPGPGFPGSKGLRGEPGNRGVSGFPGVPGLSGPQGDCYQETDEENEIGKEGTGCRLIAPPAPAGAPGPPGLPGYNGIRGQKGFPGPHGFDGVFGLKGIKGDRGTDSLPGPPGFSGQRGDRGNAGLPGLPGVPGLSGKPGFKGVQGEKGSVGDRLGAPSGEQGNTGLPGLQGLKGTQGDQGIPGLQGKVGTPGTPGNKGIKGSPGAPGVPGIPGPAALFGFPGMPGNPGTPGPQGSMGPPGPPGQRGDEGEKGPPGPAGMKGLPGELGVGGFIGVRGRHGNPGIPGLVGMHGLPGVKGIKGSPGMEGFQGMKGAKGRPAWRGLKGPSGNIGFPGVKGGNGTSGTKGDRGEQGPPGDPPKMPEMMLLTKGETGDQGVSGFKGISGLKGSKGMPGPPGQLGLPGLPGLRSFEFGDKGETGTPGVIGNQGILGEVGPDGIIGFPGFTGPRGTPGISGFPGVPGERGKYGDIGERGDTIDLPGERGTKGQGGTPGLPGQRGIHGEKGTTGDEGFRGIEGVIGEHGQTGEKGFPGQQGLVGFPGSQGFPGLPGVPGEKGSSGFPGLPGQHGFPGIRGIAGLDGLPGTKGINGQPGADIIGLKGFKGLSGGKGQPGEASTIVGMPGPAGSKGAEGDQGLPGLIGLPGTVGVRGLPGFSNKTGLLGDVGPPGPPGLPGFPGPIGRPGLPSKPGVKGVIGDLGLQGNYGAKGIPGDEGKVGVPGLAGIPGAKGLKGSAGFQGFVGVSGYRGDQGPKGLRGDIGEYGLKGPPGQPGPMSEPPLITAEQGYPGAHGIIGHQGVQGDMGPKGLVGTPGAPGQTGKEGLPGVPGWPAPPGSRGEQGPMGRTGPNGFKGASGVPGRAGLPGMPGRSVNIGYLLVKHSQTDEEPMCPVGMARLWTGYSLLYFEGQEKAHNQDLGLAGSCLQRFSTMPFLYCNPGDVCYYANRNDKSYWLSTTAPLPMMPVVEEEIRPYISRCSVCEAPAVAIAVHSQDVSIPHCPDGWRSLWIGYSFLMHTAAGDEGGGQSLSSPGSCLEDFRATPFIECNGGRGTCHYFANKYSFWLTTIDEPFQSSPPADTLKAGLIRTHISRCQVCMKNL

Target ORF information:

RefSeq Version	XM_031897041.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897041.1

ORF Insert Sequence:

ATGGCAGGAA CTCAAAGCAC CAAGAGATGT CCCACAAGCA GGAGAGAGTA CAGCTGTTTT 
CAGCTCCAAC ACTATTCAGT CACTTGGTGC ATGCTGTTTG TTGTACTGGT GACATGCTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGCC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGAT GCCAGTGTCT GCCCGAGAAA GGAAGTCGTG GACAACCAGG ACCCCTGGGA 
GGGCAAGGTA CCTCTGGCCC ACCTGGGCTA ATAGGAATAG CAGGTCTGCA GGGACGTAAG 
GGAGACAAAG GAGAACGAGG ATTTCCTGGA GTGACTGGCC CCTCTGGAGA TAAGGGCCAA 
AGTGGTGTGA CAGGTTTTCC CGGAGCTGAT GGCGTACCTG GACATACAGG TCAGGGTGGC 
CCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAC AAGGCGATGC TGGTGTGGGA 
GGTTCACATG GTTCAAGAGG CCCTCCTGGA ATTTCTGGTG GATTTGGTGC CAAAGGTCAA 
AAAGGAGAAC CGTTTCATGT ATCAATCGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAAGCAGGCT TTAATGGTAT TCAGGGTCCA AGGGGTTCTC CTGGAACAGC AGGCTTCATT 
GGGCCACAGG GTTACCGTGG ACCTCGTGGG CCACCTGGAC CTCCTGGGCC ACAGGGACCA 
CAAGGCAACA GGGGTCTTGG ATATTATGGA GAAAAAGGAG AACCTGGTGA TCCTGGTCCA 
CCTGGTGAAC AACCTCGTCA AGGAGAGAGA GAACAAAAGC ACAAAATTGA AATTCTCTTG 
CTACAATATA AGGGGGCCAA GGGGGACCAA GGAGAGAAGG GAAATCCTGG CGATAAAGGT 
CAACCAGCTG TCACAGGAGA AAAAGGAGAG GAAGGTATTA CAGGATTTCC TGGACAAAGG 
GGGTTTCCTG GTAATGATGG TAACAGTGGA GCTTCAGGAG AAATAGGGTT TCCTGGGGCA 
AACGGTGTAC CTGGATTACC TGGTACTAAA GGAAAAAAGG GAGAGGTTGG AGACCTAGGA 
CCTCAAGGAC CTGTTACTTT TGTCAATGGT CAACAAAAAA GAAAAGGCTA TGAAGGTGAA 
ATTGGATTTC CAGGACTTAA TGGGGTTTAT GGAGATGATG GAGACCCTGG AGATCCTGGT 
TTTCCAGGCC AGAATGGATC ACCAAGTAGA TTTCCAGGAC AAACAGGTGA TACAGGCTTG 
AAAGGTCAAA GGGGTCCCAA AGGTTACCAA GGTGATACAG GCTTACCAGC ATTTCAGCCT 
GGACCCCCAG GTATAGATGG AGCCCCTGGC AAAGAAGGAC CACCTGGCCA ACCAGGTTAT 
CCAGGTGGAC GCATTGGGAT GAAGGGATTA CCCGGGTTTC CTGGAAGGGA TGGACAAAGA 
GGAGCTCCTG GTTCTCGAGG TTTAAGGGGA ATCAAAGGTG AACAAGGGGA ATGCAGATGC 
TATGAAGGAG ATGAAGCTGG CAGAGGCCCT GCTGGAGAGC CTGGTCCAGT TGGTTTTCCA 
GGTTTTCAGG GTCACGCAGG CAGAAAGGGA GAAGCAGGAG ATAGAGGAGT ACAAGGGCTT 
CCAGGAGCAC CAGGCACAGT GGGTTTAGCT GGTCCTGCTG GGTTTCCTGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTATT TGCTACAGAA AAAGGTTCCA AGGGAGCACA AGGAGACTCT 
GGTTTTCCTG GTGTTCCTGG GCGTGATGGA TACCCTGGAA GAAATGGTAG AGATGGTAGT 
CCTGGCTTTC CAGGCCCTTC TGGAGATGGT ATAAAAGGAC TCCCAGGTTC TCATGGGTTT 
CCAGGACTCC CTGGTAATGC TGGTGCTCGA GGTGAGACGG GCTTTCCTGG ACCAGGATTT 
CCAGGTTCAA AAGGGCTGCG GGGAGAGCCA GGAAACAGAG GAGTGAGCGG ATTTCCAGGA 
GTTCCTGGAC TTTCAGGCCC CCAAGGTGAC TGCTATCAAG AAACTGATGA AGAAAATGAA 
ATAGGGAAAG AAGGGACTGG CTGCAGACTA ATTGCACCTC CTGCTCCAGC AGGTGCCCCA 
GGGCCACCAG GATTACCAGG ATATAACGGT ATAAGAGGCC AGAAAGGTTT TCCCGGACCA 
CATGGTTTTG ATGGCGTTTT TGGATTAAAA GGCATCAAAG GAGACCGTGG AACCGATAGT 
TTGCCAGGTC CACCAGGTTT TTCTGGACAA AGGGGAGACA GAGGTAATGC AGGACTACCT 
GGCCTACCCG GGGTACCAGG TTTGTCAGGA AAACCTGGCT TTAAAGGGGT ACAAGGAGAA 
AAAGGAAGTG TAGGTGATCG CTTAGGAGCT CCATCAGGGG AGCAAGGAAA TACTGGCCTG 
CCTGGCTTGC AAGGACTTAA AGGAACACAA GGGGATCAAG GCATACCTGG CCTACAAGGT 
AAAGTTGGAA CACCAGGAAC ACCTGGAAAC AAGGGTATTA AAGGCTCTCC CGGGGCCCCA 
GGAGTACCTG GAATACCAGG ACCTGCAGCA TTATTTGGAT TTCCAGGAAT GCCCGGAAAC 
CCAGGCACAC CAGGACCCCA AGGTTCAATG GGACCTCCAG GTCCTCCAGG ACAGAGAGGA 
GATGAAGGCG AGAAAGGGCC ACCAGGTCCA GCTGGGATGA AAGGGTTACC TGGAGAACTT 
GGTGTTGGAG GATTTATTGG TGTTAGGGGC AGACATGGAA ATCCAGGCAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGCATCAAAG GTTCCCCTGG CATGGAAGGC 
TTCCAGGGAA TGAAAGGAGC GAAGGGGAGA CCTGCATGGA GAGGACTCAA AGGACCAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG GGAAATGGAA CTTCCGGTAC AAAAGGTGAC 
CGTGGGGAAC AAGGACCACC AGGAGACCCC CCCAAAATGC CAGAAATGAT GCTTTTGACA 
AAAGGCGAAA CAGGAGATCA AGGTGTCTCG GGCTTTAAAG GAATTTCTGG ACTTAAAGGC 
AGTAAAGGTA TGCCTGGACC TCCAGGACAG CTAGGCTTAC CTGGCTTGCC TGGACTACGA 
AGCTTTGAAT TTGGAGATAA AGGAGAAACT GGTACTCCTG GGGTTATCGG TAATCAAGGA 
ATTCTAGGAG AAGTTGGTCC AGACGGAATT ATTGGCTTCC CAGGATTTAC TGGTCCCAGA 
GGTACTCCTG GTATTTCTGG CTTTCCTGGT GTTCCTGGTG AAAGAGGCAA ATATGGAGAT 
ATAGGAGAAA GAGGTGATAC TATTGATTTA CCAGGAGAAA GAGGAACAAA GGGTCAGGGT 
GGAACCCCCG GGCTGCCAGG ACAGCGAGGA ATACACGGAG AAAAAGGAAC TACAGGAGAT 
GAAGGTTTTC GGGGTATTGA GGGAGTGATA GGGGAACACG GACAAACAGG AGAAAAAGGA 
TTCCCAGGTC AACAAGGCCT TGTGGGCTTT CCAGGCTCAC AGGGTTTTCC AGGTCTGCCA 
GGTGTGCCTG GCGAGAAAGG AAGTTCTGGG TTTCCTGGGT TACCTGGTCA ACATGGTTTT 
CCTGGCATAA GAGGGATTGC TGGCCTAGAT GGTCTGCCAG GCACTAAAGG AATTAATGGA 
CAGCCAGGTG CGGATATAAT TGGTTTAAAG GGATTTAAAG GTCTAAGTGG TGGAAAGGGT 
CAACCAGGAG AGGCTAGTAC CATTGTAGGA ATGCCTGGAC CTGCAGGATC TAAAGGAGCC 
GAAGGGGATC AAGGTTTACC TGGCTTAATT GGACTTCCTG GAACAGTTGG AGTCCGTGGC 
TTGCCTGGGT TTTCTAACAA GACAGGTTTG CTAGGAGATG TTGGACCTCC AGGACCTCCT 
GGACTGCCAG GTTTTCCAGG CCCTATCGGA AGACCAGGTT TACCCTCTAA ACCTGGAGTA 
AAAGGAGTCA TAGGGGATCT GGGGTTACAA GGGAACTATG GTGCTAAAGG TATTCCTGGT 
GACGAAGGAA AAGTGGGAGT TCCTGGACTG GCTGGAATAC CTGGAGCCAA AGGTTTGAAA 
GGCAGTGCTG GCTTCCAGGG TTTTGTTGGG GTATCAGGGT ATCGAGGAGA TCAAGGACCT 
AAAGGGCTTA GAGGTGACAT CGGAGAATAT GGTTTAAAGG GTCCTCCTGG ACAACCGGGC 
CCTATGTCTG AACCTCCTCT TATTACGGCT GAACAAGGCT ACCCTGGAGC ACACGGAATT 
ATTGGCCATC AAGGTGTTCA GGGCGATATG GGCCCAAAAG GTCTTGTGGG TACTCCAGGT 
GCCCCTGGCC AAACCGGTAA AGAAGGCTTA CCAGGTGTAC CAGGCTGGCC TGCTCCTCCA 
GGCAGTCGTG GAGAACAAGG ACCCATGGGA CGAACAGGAC CTAATGGATT CAAAGGTGCC 
AGTGGTGTGC CAGGCAGGGC AGGGCTTCCT GGGATGCCCG GGAGAAGTGT CAATATTGGC 
TATCTTTTAG TCAAGCACAG TCAAACAGAT GAAGAGCCAA TGTGTCCTGT TGGTATGGCC 
CGCTTGTGGA CTGGGTATAG TCTCTTGTAT TTTGAAGGAC AGGAAAAAGC ACACAATCAA 
GACTTAGGTC TAGCTGGATC TTGTTTACAG CGCTTCAGTA CAATGCCGTT CCTGTACTGC 
AACCCAGGGG ATGTCTGTTA TTATGCCAAC CGTAACGATA AGTCTTATTG GCTTTCCACC 
ACTGCACCTC TTCCAATGAT GCCTGTGGTT GAAGAAGAAA TTAGGCCATA TATTAGCCGT 
TGCTCAGTGT GTGAGGCTCC AGCAGTAGCA ATTGCTGTTC ACAGTCAAGA TGTCAGTATC 
CCACATTGCC CAGATGGTTG GCGGAGCTTG TGGATTGGCT ACTCCTTTCT CATGCACACT 
GCCGCAGGCG ATGAGGGGGG AGGACAATCT CTCTCCTCCC CTGGAAGTTG TTTGGAAGAC 
TTCAGGGCGA CTCCATTCAT TGAATGCAAT GGTGGAAGAG GGACCTGCCA CTACTTTGCA 
AACAAATACA GTTTCTGGCT TACTACTATT GACGAGCCCT TCCAGAGCTC TCCGCCTGCA 
GATACACTAA AAGCTGGGCT GATTCGAACT CATATCAGCC GATGCCAAGT CTGCATGAAA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a2 ( XM_004911987.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912044.1 Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X1, mRNA.

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CloneID	OXa06182
Clone ID Related Accession (Same CDS sequence)	XM_004911987.4 , XM_004911987.3 , XM_002933017.2 , XM_002933017.3 , XM_031897040.1 , XM_031897041.1
Accession Version	XM_004911987.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-2(IV) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911987.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA CTCAAAGCAC CAAGAGATGT CCCACAAGCA GGAGAGAGTA CAGCTGTTTT 
CAGCTCCAAC ACTATTCAGT CACTTGGTGC ATGCTGTTTG TTGTACTGGT GACATGCTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGCC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGAT GCCAGTGTCT GCCCGAGAAA GGAAGTCGTG GACAACCAGG ACCCCTGGGA 
GGGCAAGGTA CCTCTGGCCC ACCTGGGCTA ATAGGAATAG CAGGTCTGCA GGGACGTAAG 
GGAGACAAAG GAGAACGAGG ATTTCCTGGA GTGACTGGCC CCTCTGGAGA TAAGGGCCAA 
AGTGGTGTGA CAGGTTTTCC CGGAGCTGAT GGCGTACCTG GACATACAGG TCAGGGTGGC 
CCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAC AAGGCGATGC TGGTGTGGGA 
GGTTCACATG GTTCAAGAGG CCCTCCTGGA ATTTCTGGTG GATTTGGTGC CAAAGGTCAA 
AAAGGAGAAC CGTTTCATGT ATCAATCGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAAGCAGGCT TTAATGGTAT TCAGGGTCCA AGGGGTTCTC CTGGAACAGC AGGCTTCATT 
GGGCCACAGG GTTACCGTGG ACCTCGTGGG CCACCTGGAC CTCCTGGGCC ACAGGGACCA 
CAAGGCAACA GGGGTCTTGG ATATTATGGA GAAAAAGGAG AACCTGGTGA TCCTGGTCCA 
CCTGGTGAAC AACCTCGTCA AGGAGAGAGA GAACAAAAGC ACAAAATTGA AATTCTCTTG 
CTACAATATA AGGGGGCCAA GGGGGACCAA GGAGAGAAGG GAAATCCTGG CGATAAAGGT 
CAACCAGCTG TCACAGGAGA AAAAGGAGAG GAAGGTATTA CAGGATTTCC TGGACAAAGG 
GGGTTTCCTG GTAATGATGG TAACAGTGGA GCTTCAGGAG AAATAGGGTT TCCTGGGGCA 
AACGGTGTAC CTGGATTACC TGGTACTAAA GGAAAAAAGG GAGAGGTTGG AGACCTAGGA 
CCTCAAGGAC CTGTTACTTT TGTCAATGGT CAACAAAAAA GAAAAGGCTA TGAAGGTGAA 
ATTGGATTTC CAGGACTTAA TGGGGTTTAT GGAGATGATG GAGACCCTGG AGATCCTGGT 
TTTCCAGGCC AGAATGGATC ACCAAGTAGA TTTCCAGGAC AAACAGGTGA TACAGGCTTG 
AAAGGTCAAA GGGGTCCCAA AGGTTACCAA GGTGATACAG GCTTACCAGC ATTTCAGCCT 
GGACCCCCAG GTATAGATGG AGCCCCTGGC AAAGAAGGAC CACCTGGCCA ACCAGGTTAT 
CCAGGTGGAC GCATTGGGAT GAAGGGATTA CCCGGGTTTC CTGGAAGGGA TGGACAAAGA 
GGAGCTCCTG GTTCTCGAGG TTTAAGGGGA ATCAAAGGTG AACAAGGGGA ATGCAGATGC 
TATGAAGGAG ATGAAGCTGG CAGAGGCCCT GCTGGAGAGC CTGGTCCAGT TGGTTTTCCA 
GGTTTTCAGG GTCACGCAGG CAGAAAGGGA GAAGCAGGAG ATAGAGGAGT ACAAGGGCTT 
CCAGGAGCAC CAGGCACAGT GGGTTTAGCT GGTCCTGCTG GGTTTCCTGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTATT TGCTACAGAA AAAGGTTCCA AGGGAGCACA AGGAGACTCT 
GGTTTTCCTG GTGTTCCTGG GCGTGATGGA TACCCTGGAA GAAATGGTAG AGATGGTAGT 
CCTGGCTTTC CAGGCCCTTC TGGAGATGGT ATAAAAGGAC TCCCAGGTTC TCATGGGTTT 
CCAGGACTCC CTGGTAATGC TGGTGCTCGA GGTGAGACGG GCTTTCCTGG ACCAGGATTT 
CCAGGTTCAA AAGGGCTGCG GGGAGAGCCA GGAAACAGAG GAGTGAGCGG ATTTCCAGGA 
GTTCCTGGAC TTTCAGGCCC CCAAGGTGAC TGCTATCAAG AAACTGATGA AGAAAATGAA 
ATAGGGAAAG AAGGGACTGG CTGCAGACTA ATTGCACCTC CTGCTCCAGC AGGTGCCCCA 
GGGCCACCAG GATTACCAGG ATATAACGGT ATAAGAGGCC AGAAAGGTTT TCCCGGACCA 
CATGGTTTTG ATGGCGTTTT TGGATTAAAA GGCATCAAAG GAGACCGTGG AACCGATAGT 
TTGCCAGGTC CACCAGGTTT TTCTGGACAA AGGGGAGACA GAGGTAATGC AGGACTACCT 
GGCCTACCCG GGGTACCAGG TTTGTCAGGA AAACCTGGCT TTAAAGGGGT ACAAGGAGAA 
AAAGGAAGTG TAGGTGATCG CTTAGGAGCT CCATCAGGGG AGCAAGGAAA TACTGGCCTG 
CCTGGCTTGC AAGGACTTAA AGGAACACAA GGGGATCAAG GCATACCTGG CCTACAAGGT 
AAAGTTGGAA CACCAGGAAC ACCTGGAAAC AAGGGTATTA AAGGCTCTCC CGGGGCCCCA 
GGAGTACCTG GAATACCAGG ACCTGCAGCA TTATTTGGAT TTCCAGGAAT GCCCGGAAAC 
CCAGGCACAC CAGGACCCCA AGGTTCAATG GGACCTCCAG GTCCTCCAGG ACAGAGAGGA 
GATGAAGGCG AGAAAGGGCC ACCAGGTCCA GCTGGGATGA AAGGGTTACC TGGAGAACTT 
GGTGTTGGAG GATTTATTGG TGTTAGGGGC AGACATGGAA ATCCAGGCAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGCATCAAAG GTTCCCCTGG CATGGAAGGC 
TTCCAGGGAA TGAAAGGAGC GAAGGGGAGA CCTGCATGGA GAGGACTCAA AGGACCAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG GGAAATGGAA CTTCCGGTAC AAAAGGTGAC 
CGTGGGGAAC AAGGACCACC AGGAGACCCC CCCAAAATGC CAGAAATGAT GCTTTTGACA 
AAAGGCGAAA CAGGAGATCA AGGTGTCTCG GGCTTTAAAG GAATTTCTGG ACTTAAAGGC 
AGTAAAGGTA TGCCTGGACC TCCAGGACAG CTAGGCTTAC CTGGCTTGCC TGGACTACGA 
AGCTTTGAAT TTGGAGATAA AGGAGAAACT GGTACTCCTG GGGTTATCGG TAATCAAGGA 
ATTCTAGGAG AAGTTGGTCC AGACGGAATT ATTGGCTTCC CAGGATTTAC TGGTCCCAGA 
GGTACTCCTG GTATTTCTGG CTTTCCTGGT GTTCCTGGTG AAAGAGGCAA ATATGGAGAT 
ATAGGAGAAA GAGGTGATAC TATTGATTTA CCAGGAGAAA GAGGAACAAA GGGTCAGGGT 
GGAACCCCCG GGCTGCCAGG ACAGCGAGGA ATACACGGAG AAAAAGGAAC TACAGGAGAT 
GAAGGTTTTC GGGGTATTGA GGGAGTGATA GGGGAACACG GACAAACAGG AGAAAAAGGA 
TTCCCAGGTC AACAAGGCCT TGTGGGCTTT CCAGGCTCAC AGGGTTTTCC AGGTCTGCCA 
GGTGTGCCTG GCGAGAAAGG AAGTTCTGGG TTTCCTGGGT TACCTGGTCA ACATGGTTTT 
CCTGGCATAA GAGGGATTGC TGGCCTAGAT GGTCTGCCAG GCACTAAAGG AATTAATGGA 
CAGCCAGGTG CGGATATAAT TGGTTTAAAG GGATTTAAAG GTCTAAGTGG TGGAAAGGGT 
CAACCAGGAG AGGCTAGTAC CATTGTAGGA ATGCCTGGAC CTGCAGGATC TAAAGGAGCC 
GAAGGGGATC AAGGTTTACC TGGCTTAATT GGACTTCCTG GAACAGTTGG AGTCCGTGGC 
TTGCCTGGGT TTTCTAACAA GACAGGTTTG CTAGGAGATG TTGGACCTCC AGGACCTCCT 
GGACTGCCAG GTTTTCCAGG CCCTATCGGA AGACCAGGTT TACCCTCTAA ACCTGGAGTA 
AAAGGAGTCA TAGGGGATCT GGGGTTACAA GGGAACTATG GTGCTAAAGG TATTCCTGGT 
GACGAAGGAA AAGTGGGAGT TCCTGGACTG GCTGGAATAC CTGGAGCCAA AGGTTTGAAA 
GGCAGTGCTG GCTTCCAGGG TTTTGTTGGG GTATCAGGGT ATCGAGGAGA TCAAGGACCT 
AAAGGGCTTA GAGGTGACAT CGGAGAATAT GGTTTAAAGG GTCCTCCTGG ACAACCGGGC 
CCTATGTCTG AACCTCCTCT TATTACGGCT GAACAAGGCT ACCCTGGAGC ACACGGAATT 
ATTGGCCATC AAGGTGTTCA GGGCGATATG GGCCCAAAAG GTCTTGTGGG TACTCCAGGT 
GCCCCTGGCC AAACCGGTAA AGAAGGCTTA CCAGGTGTAC CAGGCTGGCC TGCTCCTCCA 
GGCAGTCGTG GAGAACAAGG ACCCATGGGA CGAACAGGAC CTAATGGATT CAAAGGTGCC 
AGTGGTGTGC CAGGCAGGGC AGGGCTTCCT GGGATGCCCG GGAGAAGTGT CAATATTGGC 
TATCTTTTAG TCAAGCACAG TCAAACAGAT GAAGAGCCAA TGTGTCCTGT TGGTATGGCC 
CGCTTGTGGA CTGGGTATAG TCTCTTGTAT TTTGAAGGAC AGGAAAAAGC ACACAATCAA 
GACTTAGGTC TAGCTGGATC TTGTTTACAG CGCTTCAGTA CAATGCCGTT CCTGTACTGC 
AACCCAGGGG ATGTCTGTTA TTATGCCAAC CGTAACGATA AGTCTTATTG GCTTTCCACC 
ACTGCACCTC TTCCAATGAT GCCTGTGGTT GAAGAAGAAA TTAGGCCATA TATTAGCCGT 
TGCTCAGTGT GTGAGGCTCC AGCAGTAGCA ATTGCTGTTC ACAGTCAAGA TGTCAGTATC 
CCACATTGCC CAGATGGTTG GCGGAGCTTG TGGATTGGCT ACTCCTTTCT CATGCACACT 
GCCGCAGGCG ATGAGGGGGG AGGACAATCT CTCTCCTCCC CTGGAAGTTG TTTGGAAGAC 
TTCAGGGCGA CTCCATTCAT TGAATGCAAT GGTGGAAGAG GGACCTGCCA CTACTTTGCA 
AACAAATACA GTTTCTGGCT TACTACTATT GACGAGCCCT TCCAGAGCTC TCCGCCTGCA 
GATACACTAA AAGCTGGGCT GATTCGAACT CATATCAGCC GATGCCAAGT CTGCATGAAA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912044.1
CDS	151..5319
Translation	MAGTQSTKRCPTSRREYSCFQLQHYSVTWCMLFVVLVTCFAGNAHAGDKSYTGPCGGRDCSQGCQCLPEKGSRGQPGPLGGQGTSGPPGLIGIAGLQGRKGDKGERGFPGVTGPSGDKGQSGVTGFPGADGVPGHTGQGGPRGKPGHDGCNGTQGDAGVGGSHGSRGPPGISGGFGAKGQKGEPFHVSIEVKNRLRGDPGEAGFNGIQGPRGSPGTAGFIGPQGYRGPRGPPGPPGPQGPQGNRGLGYYGEKGEPGDPGPPGEQPRQGEREQKHKIEILLLQYKGAKGDQGEKGNPGDKGQPAVTGEKGEEGITGFPGQRGFPGNDGNSGASGEIGFPGANGVPGLPGTKGKKGEVGDLGPQGPVTFVNGQQKRKGYEGEIGFPGLNGVYGDDGDPGDPGFPGQNGSPSRFPGQTGDTGLKGQRGPKGYQGDTGLPAFQPGPPGIDGAPGKEGPPGQPGYPGGRIGMKGLPGFPGRDGQRGAPGSRGLRGIKGEQGECRCYEGDEAGRGPAGEPGPVGFPGFQGHAGRKGEAGDRGVQGLPGAPGTVGLAGPAGFPGPAGEKGDKVFATEKGSKGAQGDSGFPGVPGRDGYPGRNGRDGSPGFPGPSGDGIKGLPGSHGFPGLPGNAGARGETGFPGPGFPGSKGLRGEPGNRGVSGFPGVPGLSGPQGDCYQETDEENEIGKEGTGCRLIAPPAPAGAPGPPGLPGYNGIRGQKGFPGPHGFDGVFGLKGIKGDRGTDSLPGPPGFSGQRGDRGNAGLPGLPGVPGLSGKPGFKGVQGEKGSVGDRLGAPSGEQGNTGLPGLQGLKGTQGDQGIPGLQGKVGTPGTPGNKGIKGSPGAPGVPGIPGPAALFGFPGMPGNPGTPGPQGSMGPPGPPGQRGDEGEKGPPGPAGMKGLPGELGVGGFIGVRGRHGNPGIPGLVGMHGLPGVKGIKGSPGMEGFQGMKGAKGRPAWRGLKGPSGNIGFPGVKGGNGTSGTKGDRGEQGPPGDPPKMPEMMLLTKGETGDQGVSGFKGISGLKGSKGMPGPPGQLGLPGLPGLRSFEFGDKGETGTPGVIGNQGILGEVGPDGIIGFPGFTGPRGTPGISGFPGVPGERGKYGDIGERGDTIDLPGERGTKGQGGTPGLPGQRGIHGEKGTTGDEGFRGIEGVIGEHGQTGEKGFPGQQGLVGFPGSQGFPGLPGVPGEKGSSGFPGLPGQHGFPGIRGIAGLDGLPGTKGINGQPGADIIGLKGFKGLSGGKGQPGEASTIVGMPGPAGSKGAEGDQGLPGLIGLPGTVGVRGLPGFSNKTGLLGDVGPPGPPGLPGFPGPIGRPGLPSKPGVKGVIGDLGLQGNYGAKGIPGDEGKVGVPGLAGIPGAKGLKGSAGFQGFVGVSGYRGDQGPKGLRGDIGEYGLKGPPGQPGPMSEPPLITAEQGYPGAHGIIGHQGVQGDMGPKGLVGTPGAPGQTGKEGLPGVPGWPAPPGSRGEQGPMGRTGPNGFKGASGVPGRAGLPGMPGRSVNIGYLLVKHSQTDEEPMCPVGMARLWTGYSLLYFEGQEKAHNQDLGLAGSCLQRFSTMPFLYCNPGDVCYYANRNDKSYWLSTTAPLPMMPVVEEEIRPYISRCSVCEAPAVAIAVHSQDVSIPHCPDGWRSLWIGYSFLMHTAAGDEGGGQSLSSPGSCLEDFRATPFIECNGGRGTCHYFANKYSFWLTTIDEPFQSSPPADTLKAGLIRTHISRCQVCMKNL

Target ORF information:

RefSeq Version	XM_004911987.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911987.3

ORF Insert Sequence:

ATGGCAGGAA CTCAAAGCAC CAAGAGATGT CCCACAAGCA GGAGAGAGTA CAGCTGTTTT 
CAGCTCCAAC ACTATTCAGT CACTTGGTGC ATGCTGTTTG TTGTACTGGT GACATGCTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGCC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGAT GCCAGTGTCT GCCCGAGAAA GGAAGTCGTG GACAACCAGG ACCCCTGGGA 
GGGCAAGGTA CCTCTGGCCC ACCTGGGCTA ATAGGAATAG CAGGTCTGCA GGGACGTAAG 
GGAGACAAAG GAGAACGAGG ATTTCCTGGA GTGACTGGCC CCTCTGGAGA TAAGGGCCAA 
AGTGGTGTGA CAGGTTTTCC CGGAGCTGAT GGCGTACCTG GACATACAGG TCAGGGTGGC 
CCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAC AAGGCGATGC TGGTGTGGGA 
GGTTCACATG GTTCAAGAGG CCCTCCTGGA ATTTCTGGTG GATTTGGTGC CAAAGGTCAA 
AAAGGAGAAC CGTTTCATGT ATCAATCGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAAGCAGGCT TTAATGGTAT TCAGGGTCCA AGGGGTTCTC CTGGAACAGC AGGCTTCATT 
GGGCCACAGG GTTACCGTGG ACCTCGTGGG CCACCTGGAC CTCCTGGGCC ACAGGGACCA 
CAAGGCAACA GGGGTCTTGG ATATTATGGA GAAAAAGGAG AACCTGGTGA TCCTGGTCCA 
CCTGGTGAAC AACCTCGTCA AGGAGAGAGA GAACAAAAGC ACAAAATTGA AATTCTCTTG 
CTACAATATA AGGGGGCCAA GGGGGACCAA GGAGAGAAGG GAAATCCTGG CGATAAAGGT 
CAACCAGCTG TCACAGGAGA AAAAGGAGAG GAAGGTATTA CAGGATTTCC TGGACAAAGG 
GGGTTTCCTG GTAATGATGG TAACAGTGGA GCTTCAGGAG AAATAGGGTT TCCTGGGGCA 
AACGGTGTAC CTGGATTACC TGGTACTAAA GGAAAAAAGG GAGAGGTTGG AGACCTAGGA 
CCTCAAGGAC CTGTTACTTT TGTCAATGGT CAACAAAAAA GAAAAGGCTA TGAAGGTGAA 
ATTGGATTTC CAGGACTTAA TGGGGTTTAT GGAGATGATG GAGACCCTGG AGATCCTGGT 
TTTCCAGGCC AGAATGGATC ACCAAGTAGA TTTCCAGGAC AAACAGGTGA TACAGGCTTG 
AAAGGTCAAA GGGGTCCCAA AGGTTACCAA GGTGATACAG GCTTACCAGC ATTTCAGCCT 
GGACCCCCAG GTATAGATGG AGCCCCTGGC AAAGAAGGAC CACCTGGCCA ACCAGGTTAT 
CCAGGTGGAC GCATTGGGAT GAAGGGATTA CCCGGGTTTC CTGGAAGGGA TGGACAAAGA 
GGAGCTCCTG GTTCTCGAGG TTTAAGGGGA ATCAAAGGTG AACAAGGGGA ATGCAGATGC 
TATGAAGGAG ATGAAGCTGG CAGAGGCCCT GCTGGAGAGC CTGGTCCAGT TGGTTTTCCA 
GGTTTTCAGG GTCACGCAGG CAGAAAGGGA GAAGCAGGAG ATAGAGGAGT ACAAGGGCTT 
CCAGGAGCAC CAGGCACAGT GGGTTTAGCT GGTCCTGCTG GGTTTCCTGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTATT TGCTACAGAA AAAGGTTCCA AGGGAGCACA AGGAGACTCT 
GGTTTTCCTG GTGTTCCTGG GCGTGATGGA TACCCTGGAA GAAATGGTAG AGATGGTAGT 
CCTGGCTTTC CAGGCCCTTC TGGAGATGGT ATAAAAGGAC TCCCAGGTTC TCATGGGTTT 
CCAGGACTCC CTGGTAATGC TGGTGCTCGA GGTGAGACGG GCTTTCCTGG ACCAGGATTT 
CCAGGTTCAA AAGGGCTGCG GGGAGAGCCA GGAAACAGAG GAGTGAGCGG ATTTCCAGGA 
GTTCCTGGAC TTTCAGGCCC CCAAGGTGAC TGCTATCAAG AAACTGATGA AGAAAATGAA 
ATAGGGAAAG AAGGGACTGG CTGCAGACTA ATTGCACCTC CTGCTCCAGC AGGTGCCCCA 
GGGCCACCAG GATTACCAGG ATATAACGGT ATAAGAGGCC AGAAAGGTTT TCCCGGACCA 
CATGGTTTTG ATGGCGTTTT TGGATTAAAA GGCATCAAAG GAGACCGTGG AACCGATAGT 
TTGCCAGGTC CACCAGGTTT TTCTGGACAA AGGGGAGACA GAGGTAATGC AGGACTACCT 
GGCCTACCCG GGGTACCAGG TTTGTCAGGA AAACCTGGCT TTAAAGGGGT ACAAGGAGAA 
AAAGGAAGTG TAGGTGATCG CTTAGGAGCT CCATCAGGGG AGCAAGGAAA TACTGGCCTG 
CCTGGCTTGC AAGGACTTAA AGGAACACAA GGGGATCAAG GCATACCTGG CCTACAAGGT 
AAAGTTGGAA CACCAGGAAC ACCTGGAAAC AAGGGTATTA AAGGCTCTCC CGGGGCCCCA 
GGAGTACCTG GAATACCAGG ACCTGCAGCA TTATTTGGAT TTCCAGGAAT GCCCGGAAAC 
CCAGGCACAC CAGGACCCCA AGGTTCAATG GGACCTCCAG GTCCTCCAGG ACAGAGAGGA 
GATGAAGGCG AGAAAGGGCC ACCAGGTCCA GCTGGGATGA AAGGGTTACC TGGAGAACTT 
GGTGTTGGAG GATTTATTGG TGTTAGGGGC AGACATGGAA ATCCAGGCAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGCATCAAAG GTTCCCCTGG CATGGAAGGC 
TTCCAGGGAA TGAAAGGAGC GAAGGGGAGA CCTGCATGGA GAGGACTCAA AGGACCAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG GGAAATGGAA CTTCCGGTAC AAAAGGTGAC 
CGTGGGGAAC AAGGACCACC AGGAGACCCC CCCAAAATGC CAGAAATGAT GCTTTTGACA 
AAAGGCGAAA CAGGAGATCA AGGTGTCTCG GGCTTTAAAG GAATTTCTGG ACTTAAAGGC 
AGTAAAGGTA TGCCTGGACC TCCAGGACAG CTAGGCTTAC CTGGCTTGCC TGGACTACGA 
AGCTTTGAAT TTGGAGATAA AGGAGAAACT GGTACTCCTG GGGTTATCGG TAATCAAGGA 
ATTCTAGGAG AAGTTGGTCC AGACGGAATT ATTGGCTTCC CAGGATTTAC TGGTCCCAGA 
GGTACTCCTG GTATTTCTGG CTTTCCTGGT GTTCCTGGTG AAAGAGGCAA ATATGGAGAT 
ATAGGAGAAA GAGGTGATAC TATTGATTTA CCAGGAGAAA GAGGAACAAA GGGTCAGGGT 
GGAACCCCCG GGCTGCCAGG ACAGCGAGGA ATACACGGAG AAAAAGGAAC TACAGGAGAT 
GAAGGTTTTC GGGGTATTGA GGGAGTGATA GGGGAACACG GACAAACAGG AGAAAAAGGA 
TTCCCAGGTC AACAAGGCCT TGTGGGCTTT CCAGGCTCAC AGGGTTTTCC AGGTCTGCCA 
GGTGTGCCTG GCGAGAAAGG AAGTTCTGGG TTTCCTGGGT TACCTGGTCA ACATGGTTTT 
CCTGGCATAA GAGGGATTGC TGGCCTAGAT GGTCTGCCAG GCACTAAAGG AATTAATGGA 
CAGCCAGGTG CGGATATAAT TGGTTTAAAG GGATTTAAAG GTCTAAGTGG TGGAAAGGGT 
CAACCAGGAG AGGCTAGTAC CATTGTAGGA ATGCCTGGAC CTGCAGGATC TAAAGGAGCC 
GAAGGGGATC AAGGTTTACC TGGCTTAATT GGACTTCCTG GAACAGTTGG AGTCCGTGGC 
TTGCCTGGGT TTTCTAACAA GACAGGTTTG CTAGGAGATG TTGGACCTCC AGGACCTCCT 
GGACTGCCAG GTTTTCCAGG CCCTATCGGA AGACCAGGTT TACCCTCTAA ACCTGGAGTA 
AAAGGAGTCA TAGGGGATCT GGGGTTACAA GGGAACTATG GTGCTAAAGG TATTCCTGGT 
GACGAAGGAA AAGTGGGAGT TCCTGGACTG GCTGGAATAC CTGGAGCCAA AGGTTTGAAA 
GGCAGTGCTG GCTTCCAGGG TTTTGTTGGG GTATCAGGGT ATCGAGGAGA TCAAGGACCT 
AAAGGGCTTA GAGGTGACAT CGGAGAATAT GGTTTAAAGG GTCCTCCTGG ACAACCGGGC 
CCTATGTCTG AACCTCCTCT TATTACGGCT GAACAAGGCT ACCCTGGAGC ACACGGAATT 
ATTGGCCATC AAGGTGTTCA GGGCGATATG GGCCCAAAAG GTCTTGTGGG TACTCCAGGT 
GCCCCTGGCC AAACCGGTAA AGAAGGCTTA CCAGGTGTAC CAGGCTGGCC TGCTCCTCCA 
GGCAGTCGTG GAGAACAAGG ACCCATGGGA CGAACAGGAC CTAATGGATT CAAAGGTGCC 
AGTGGTGTGC CAGGCAGGGC AGGGCTTCCT GGGATGCCCG GGAGAAGTGT CAATATTGGC 
TATCTTTTAG TCAAGCACAG TCAAACAGAT GAAGAGCCAA TGTGTCCTGT TGGTATGGCC 
CGCTTGTGGA CTGGGTATAG TCTCTTGTAT TTTGAAGGAC AGGAAAAAGC ACACAATCAA 
GACTTAGGTC TAGCTGGATC TTGTTTACAG CGCTTCAGTA CAATGCCGTT CCTGTACTGC 
AACCCAGGGG ATGTCTGTTA TTATGCCAAC CGTAACGATA AGTCTTATTG GCTTTCCACC 
ACTGCACCTC TTCCAATGAT GCCTGTGGTT GAAGAAGAAA TTAGGCCATA TATTAGCCGT 
TGCTCAGTGT GTGAGGCTCC AGCAGTAGCA ATTGCTGTTC ACAGTCAAGA TGTCAGTATC 
CCACATTGCC CAGATGGTTG GCGGAGCTTG TGGATTGGCT ACTCCTTTCT CATGCACACT 
GCCGCAGGCG ATGAGGGGGG AGGACAATCT CTCTCCTCCC CTGGAAGTTG TTTGGAAGAC 
TTCAGGGCGA CTCCATTCAT TGAATGCAAT GGTGGAAGAG GGACCTGCCA CTACTTTGCA 
AACAAATACA GTTTCTGGCT TACTACTATT GACGAGCCCT TCCAGAGCTC TCCGCCTGCA 
GATACACTAA AAGCTGGGCT GATTCGAACT CATATCAGCC GATGCCAAGT CTGCATGAAA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a2 ( XM_002933017.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933063.2 Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X2, mRNA.

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CloneID	OXa06182
Clone ID Related Accession (Same CDS sequence)	XM_004911987.4 , XM_004911987.3 , XM_002933017.2 , XM_002933017.3 , XM_031897040.1 , XM_031897041.1
Accession Version	XM_002933017.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-2(IV) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 18, 2013 this sequence version replaced XM_002933017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA CTCAAAGCAC CAAGAGATGT CCCACAAGCA GGAGAGAGTA CAGCTGTTTT 
CAGCTCCAAC ACTATTCAGT CACTTGGTGC ATGCTGTTTG TTGTACTGGT GACATGCTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGCC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGAT GCCAGTGTCT GCCCGAGAAA GGAAGTCGTG GACAACCAGG ACCCCTGGGA 
GGGCAAGGTA CCTCTGGCCC ACCTGGGCTA ATAGGAATAG CAGGTCTGCA GGGACGTAAG 
GGAGACAAAG GAGAACGAGG ATTTCCTGGA GTGACTGGCC CCTCTGGAGA TAAGGGCCAA 
AGTGGTGTGA CAGGTTTTCC CGGAGCTGAT GGCGTACCTG GACATACAGG TCAGGGTGGC 
CCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAC AAGGCGATGC TGGTGTGGGA 
GGTTCACATG GTTCAAGAGG CCCTCCTGGA ATTTCTGGTG GATTTGGTGC CAAAGGTCAA 
AAAGGAGAAC CGTTTCATGT ATCAATCGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAAGCAGGCT TTAATGGTAT TCAGGGTCCA AGGGGTTCTC CTGGAACAGC AGGCTTCATT 
GGGCCACAGG GTTACCGTGG ACCTCGTGGG CCACCTGGAC CTCCTGGGCC ACAGGGACCA 
CAAGGCAACA GGGGTCTTGG ATATTATGGA GAAAAAGGAG AACCTGGTGA TCCTGGTCCA 
CCTGGTGAAC AACCTCGTCA AGGAGAGAGA GAACAAAAGC ACAAAATTGA AATTCTCTTG 
CTACAATATA AGGGGGCCAA GGGGGACCAA GGAGAGAAGG GAAATCCTGG CGATAAAGGT 
CAACCAGCTG TCACAGGAGA AAAAGGAGAG GAAGGTATTA CAGGATTTCC TGGACAAAGG 
GGGTTTCCTG GTAATGATGG TAACAGTGGA GCTTCAGGAG AAATAGGGTT TCCTGGGGCA 
AACGGTGTAC CTGGATTACC TGGTACTAAA GGAAAAAAGG GAGAGGTTGG AGACCTAGGA 
CCTCAAGGAC CTGTTACTTT TGTCAATGGT CAACAAAAAA GAAAAGGCTA TGAAGGTGAA 
ATTGGATTTC CAGGACTTAA TGGGGTTTAT GGAGATGATG GAGACCCTGG AGATCCTGGT 
TTTCCAGGCC AGAATGGATC ACCAAGTAGA TTTCCAGGAC AAACAGGTGA TACAGGCTTG 
AAAGGTCAAA GGGGTCCCAA AGGTTACCAA GGTGATACAG GCTTACCAGC ATTTCAGCCT 
GGACCCCCAG GTATAGATGG AGCCCCTGGC AAAGAAGGAC CACCTGGCCA ACCAGGTTAT 
CCAGGTGGAC GCATTGGGAT GAAGGGATTA CCCGGGTTTC CTGGAAGGGA TGGACAAAGA 
GGAGCTCCTG GTTCTCGAGG TTTAAGGGGA ATCAAAGGTG AACAAGGGGA ATGCAGATGC 
TATGAAGGAG ATGAAGCTGG CAGAGGCCCT GCTGGAGAGC CTGGTCCAGT TGGTTTTCCA 
GGTTTTCAGG GTCACGCAGG CAGAAAGGGA GAAGCAGGAG ATAGAGGAGT ACAAGGGCTT 
CCAGGAGCAC CAGGCACAGT GGGTTTAGCT GGTCCTGCTG GGTTTCCTGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTATT TGCTACAGAA AAAGGTTCCA AGGGAGCACA AGGAGACTCT 
GGTTTTCCTG GTGTTCCTGG GCGTGATGGA TACCCTGGAA GAAATGGTAG AGATGGTAGT 
CCTGGCTTTC CAGGCCCTTC TGGAGATGGT ATAAAAGGAC TCCCAGGTTC TCATGGGTTT 
CCAGGACTCC CTGGTAATGC TGGTGCTCGA GGTGAGACGG GCTTTCCTGG ACCAGGATTT 
CCAGGTTCAA AAGGGCTGCG GGGAGAGCCA GGAAACAGAG GAGTGAGCGG ATTTCCAGGA 
GTTCCTGGAC TTTCAGGCCC CCAAGGTGAC TGCTATCAAG AAACTGATGA AGAAAATGAA 
ATAGGGAAAG AAGGGACTGG CTGCAGACTA ATTGCACCTC CTGCTCCAGC AGGTGCCCCA 
GGGCCACCAG GATTACCAGG ATATAACGGT ATAAGAGGCC AGAAAGGTTT TCCCGGACCA 
CATGGTTTTG ATGGCGTTTT TGGATTAAAA GGCATCAAAG GAGACCGTGG AACCGATAGT 
TTGCCAGGTC CACCAGGTTT TTCTGGACAA AGGGGAGACA GAGGTAATGC AGGACTACCT 
GGCCTACCCG GGGTACCAGG TTTGTCAGGA AAACCTGGCT TTAAAGGGGT ACAAGGAGAA 
AAAGGAAGTG TAGGTGATCG CTTAGGAGCT CCATCAGGGG AGCAAGGAAA TACTGGCCTG 
CCTGGCTTGC AAGGACTTAA AGGAACACAA GGGGATCAAG GCATACCTGG CCTACAAGGT 
AAAGTTGGAA CACCAGGAAC ACCTGGAAAC AAGGGTATTA AAGGCTCTCC CGGGGCCCCA 
GGAGTACCTG GAATACCAGG ACCTGCAGCA TTATTTGGAT TTCCAGGAAT GCCCGGAAAC 
CCAGGCACAC CAGGACCCCA AGGTTCAATG GGACCTCCAG GTCCTCCAGG ACAGAGAGGA 
GATGAAGGCG AGAAAGGGCC ACCAGGTCCA GCTGGGATGA AAGGGTTACC TGGAGAACTT 
GGTGTTGGAG GATTTATTGG TGTTAGGGGC AGACATGGAA ATCCAGGCAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGCATCAAAG GTTCCCCTGG CATGGAAGGC 
TTCCAGGGAA TGAAAGGAGC GAAGGGGAGA CCTGCATGGA GAGGACTCAA AGGACCAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG GGAAATGGAA CTTCCGGTAC AAAAGGTGAC 
CGTGGGGAAC AAGGACCACC AGGAGACCCC CCCAAAATGC CAGAAATGAT GCTTTTGACA 
AAAGGCGAAA CAGGAGATCA AGGTGTCTCG GGCTTTAAAG GAATTTCTGG ACTTAAAGGC 
AGTAAAGGTA TGCCTGGACC TCCAGGACAG CTAGGCTTAC CTGGCTTGCC TGGACTACGA 
AGCTTTGAAT TTGGAGATAA AGGAGAAACT GGTACTCCTG GGGTTATCGG TAATCAAGGA 
ATTCTAGGAG AAGTTGGTCC AGACGGAATT ATTGGCTTCC CAGGATTTAC TGGTCCCAGA 
GGTACTCCTG GTATTTCTGG CTTTCCTGGT GTTCCTGGTG AAAGAGGCAA ATATGGAGAT 
ATAGGAGAAA GAGGTGATAC TATTGATTTA CCAGGAGAAA GAGGAACAAA GGGTCAGGGT 
GGAACCCCCG GGCTGCCAGG ACAGCGAGGA ATACACGGAG AAAAAGGAAC TACAGGAGAT 
GAAGGTTTTC GGGGTATTGA GGGAGTGATA GGGGAACACG GACAAACAGG AGAAAAAGGA 
TTCCCAGGTC AACAAGGCCT TGTGGGCTTT CCAGGCTCAC AGGGTTTTCC AGGTCTGCCA 
GGTGTGCCTG GCGAGAAAGG AAGTTCTGGG TTTCCTGGGT TACCTGGTCA ACATGGTTTT 
CCTGGCATAA GAGGGATTGC TGGCCTAGAT GGTCTGCCAG GCACTAAAGG AATTAATGGA 
CAGCCAGGTG CGGATATAAT TGGTTTAAAG GGATTTAAAG GTCTAAGTGG TGGAAAGGGT 
CAACCAGGAG AGGCTAGTAC CATTGTAGGA ATGCCTGGAC CTGCAGGATC TAAAGGAGCC 
GAAGGGGATC AAGGTTTACC TGGCTTAATT GGACTTCCTG GAACAGTTGG AGTCCGTGGC 
TTGCCTGGGT TTTCTAACAA GACAGGTTTG CTAGGAGATG TTGGACCTCC AGGACCTCCT 
GGACTGCCAG GTTTTCCAGG CCCTATCGGA AGACCAGGTT TACCCTCTAA ACCTGGAGTA 
AAAGGAGTCA TAGGGGATCT GGGGTTACAA GGGAACTATG GTGCTAAAGG TATTCCTGGT 
GACGAAGGAA AAGTGGGAGT TCCTGGACTG GCTGGAATAC CTGGAGCCAA AGGTTTGAAA 
GGCAGTGCTG GCTTCCAGGG TTTTGTTGGG GTATCAGGGT ATCGAGGAGA TCAAGGACCT 
AAAGGGCTTA GAGGTGACAT CGGAGAATAT GGTTTAAAGG GTCCTCCTGG ACAACCGGGC 
CCTATGTCTG AACCTCCTCT TATTACGGCT GAACAAGGCT ACCCTGGAGC ACACGGAATT 
ATTGGCCATC AAGGTGTTCA GGGCGATATG GGCCCAAAAG GTCTTGTGGG TACTCCAGGT 
GCCCCTGGCC AAACCGGTAA AGAAGGCTTA CCAGGTGTAC CAGGCTGGCC TGCTCCTCCA 
GGCAGTCGTG GAGAACAAGG ACCCATGGGA CGAACAGGAC CTAATGGATT CAAAGGTGCC 
AGTGGTGTGC CAGGCAGGGC AGGGCTTCCT GGGATGCCCG GGAGAAGTGT CAATATTGGC 
TATCTTTTAG TCAAGCACAG TCAAACAGAT GAAGAGCCAA TGTGTCCTGT TGGTATGGCC 
CGCTTGTGGA CTGGGTATAG TCTCTTGTAT TTTGAAGGAC AGGAAAAAGC ACACAATCAA 
GACTTAGGTC TAGCTGGATC TTGTTTACAG CGCTTCAGTA CAATGCCGTT CCTGTACTGC 
AACCCAGGGG ATGTCTGTTA TTATGCCAAC CGTAACGATA AGTCTTATTG GCTTTCCACC 
ACTGCACCTC TTCCAATGAT GCCTGTGGTT GAAGAAGAAA TTAGGCCATA TATTAGCCGT 
TGCTCAGTGT GTGAGGCTCC AGCAGTAGCA ATTGCTGTTC ACAGTCAAGA TGTCAGTATC 
CCACATTGCC CAGATGGTTG GCGGAGCTTG TGGATTGGCT ACTCCTTTCT CATGCACACT 
GCCGCAGGCG ATGAGGGGGG AGGACAATCT CTCTCCTCCC CTGGAAGTTG TTTGGAAGAC 
TTCAGGGCGA CTCCATTCAT TGAATGCAAT GGTGGAAGAG GGACCTGCCA CTACTTTGCA 
AACAAATACA GTTTCTGGCT TACTACTATT GACGAGCCCT TCCAGAGCTC TCCGCCTGCA 
GATACACTAA AAGCTGGGCT GATTCGAACT CATATCAGCC GATGCCAAGT CTGCATGAAA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933063.2
CDS	126..5294
Translation	MAGTQSTKRCPTSRREYSCFQLQHYSVTWCMLFVVLVTCFAGNAHAGDKSYTGPCGGRDCSQGCQCLPEKGSRGQPGPLGGQGTSGPPGLIGIAGLQGRKGDKGERGFPGVTGPSGDKGQSGVTGFPGADGVPGHTGQGGPRGKPGHDGCNGTQGDAGVGGSHGSRGPPGISGGFGAKGQKGEPFHVSIEVKNRLRGDPGEAGFNGIQGPRGSPGTAGFIGPQGYRGPRGPPGPPGPQGPQGNRGLGYYGEKGEPGDPGPPGEQPRQGEREQKHKIEILLLQYKGAKGDQGEKGNPGDKGQPAVTGEKGEEGITGFPGQRGFPGNDGNSGASGEIGFPGANGVPGLPGTKGKKGEVGDLGPQGPVTFVNGQQKRKGYEGEIGFPGLNGVYGDDGDPGDPGFPGQNGSPSRFPGQTGDTGLKGQRGPKGYQGDTGLPAFQPGPPGIDGAPGKEGPPGQPGYPGGRIGMKGLPGFPGRDGQRGAPGSRGLRGIKGEQGECRCYEGDEAGRGPAGEPGPVGFPGFQGHAGRKGEAGDRGVQGLPGAPGTVGLAGPAGFPGPAGEKGDKVFATEKGSKGAQGDSGFPGVPGRDGYPGRNGRDGSPGFPGPSGDGIKGLPGSHGFPGLPGNAGARGETGFPGPGFPGSKGLRGEPGNRGVSGFPGVPGLSGPQGDCYQETDEENEIGKEGTGCRLIAPPAPAGAPGPPGLPGYNGIRGQKGFPGPHGFDGVFGLKGIKGDRGTDSLPGPPGFSGQRGDRGNAGLPGLPGVPGLSGKPGFKGVQGEKGSVGDRLGAPSGEQGNTGLPGLQGLKGTQGDQGIPGLQGKVGTPGTPGNKGIKGSPGAPGVPGIPGPAALFGFPGMPGNPGTPGPQGSMGPPGPPGQRGDEGEKGPPGPAGMKGLPGELGVGGFIGVRGRHGNPGIPGLVGMHGLPGVKGIKGSPGMEGFQGMKGAKGRPAWRGLKGPSGNIGFPGVKGGNGTSGTKGDRGEQGPPGDPPKMPEMMLLTKGETGDQGVSGFKGISGLKGSKGMPGPPGQLGLPGLPGLRSFEFGDKGETGTPGVIGNQGILGEVGPDGIIGFPGFTGPRGTPGISGFPGVPGERGKYGDIGERGDTIDLPGERGTKGQGGTPGLPGQRGIHGEKGTTGDEGFRGIEGVIGEHGQTGEKGFPGQQGLVGFPGSQGFPGLPGVPGEKGSSGFPGLPGQHGFPGIRGIAGLDGLPGTKGINGQPGADIIGLKGFKGLSGGKGQPGEASTIVGMPGPAGSKGAEGDQGLPGLIGLPGTVGVRGLPGFSNKTGLLGDVGPPGPPGLPGFPGPIGRPGLPSKPGVKGVIGDLGLQGNYGAKGIPGDEGKVGVPGLAGIPGAKGLKGSAGFQGFVGVSGYRGDQGPKGLRGDIGEYGLKGPPGQPGPMSEPPLITAEQGYPGAHGIIGHQGVQGDMGPKGLVGTPGAPGQTGKEGLPGVPGWPAPPGSRGEQGPMGRTGPNGFKGASGVPGRAGLPGMPGRSVNIGYLLVKHSQTDEEPMCPVGMARLWTGYSLLYFEGQEKAHNQDLGLAGSCLQRFSTMPFLYCNPGDVCYYANRNDKSYWLSTTAPLPMMPVVEEEIRPYISRCSVCEAPAVAIAVHSQDVSIPHCPDGWRSLWIGYSFLMHTAAGDEGGGQSLSSPGSCLEDFRATPFIECNGGRGTCHYFANKYSFWLTTIDEPFQSSPPADTLKAGLIRTHISRCQVCMKNL

Target ORF information:

RefSeq Version	XM_002933017.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type IV, alpha 2 (col4a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933017.2

ORF Insert Sequence:

ATGGCAGGAA CTCAAAGCAC CAAGAGATGT CCCACAAGCA GGAGAGAGTA CAGCTGTTTT 
CAGCTCCAAC ACTATTCAGT CACTTGGTGC ATGCTGTTTG TTGTACTGGT GACATGCTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGCC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGAT GCCAGTGTCT GCCCGAGAAA GGAAGTCGTG GACAACCAGG ACCCCTGGGA 
GGGCAAGGTA CCTCTGGCCC ACCTGGGCTA ATAGGAATAG CAGGTCTGCA GGGACGTAAG 
GGAGACAAAG GAGAACGAGG ATTTCCTGGA GTGACTGGCC CCTCTGGAGA TAAGGGCCAA 
AGTGGTGTGA CAGGTTTTCC CGGAGCTGAT GGCGTACCTG GACATACAGG TCAGGGTGGC 
CCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAC AAGGCGATGC TGGTGTGGGA 
GGTTCACATG GTTCAAGAGG CCCTCCTGGA ATTTCTGGTG GATTTGGTGC CAAAGGTCAA 
AAAGGAGAAC CGTTTCATGT ATCAATCGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAAGCAGGCT TTAATGGTAT TCAGGGTCCA AGGGGTTCTC CTGGAACAGC AGGCTTCATT 
GGGCCACAGG GTTACCGTGG ACCTCGTGGG CCACCTGGAC CTCCTGGGCC ACAGGGACCA 
CAAGGCAACA GGGGTCTTGG ATATTATGGA GAAAAAGGAG AACCTGGTGA TCCTGGTCCA 
CCTGGTGAAC AACCTCGTCA AGGAGAGAGA GAACAAAAGC ACAAAATTGA AATTCTCTTG 
CTACAATATA AGGGGGCCAA GGGGGACCAA GGAGAGAAGG GAAATCCTGG CGATAAAGGT 
CAACCAGCTG TCACAGGAGA AAAAGGAGAG GAAGGTATTA CAGGATTTCC TGGACAAAGG 
GGGTTTCCTG GTAATGATGG TAACAGTGGA GCTTCAGGAG AAATAGGGTT TCCTGGGGCA 
AACGGTGTAC CTGGATTACC TGGTACTAAA GGAAAAAAGG GAGAGGTTGG AGACCTAGGA 
CCTCAAGGAC CTGTTACTTT TGTCAATGGT CAACAAAAAA GAAAAGGCTA TGAAGGTGAA 
ATTGGATTTC CAGGACTTAA TGGGGTTTAT GGAGATGATG GAGACCCTGG AGATCCTGGT 
TTTCCAGGCC AGAATGGATC ACCAAGTAGA TTTCCAGGAC AAACAGGTGA TACAGGCTTG 
AAAGGTCAAA GGGGTCCCAA AGGTTACCAA GGTGATACAG GCTTACCAGC ATTTCAGCCT 
GGACCCCCAG GTATAGATGG AGCCCCTGGC AAAGAAGGAC CACCTGGCCA ACCAGGTTAT 
CCAGGTGGAC GCATTGGGAT GAAGGGATTA CCCGGGTTTC CTGGAAGGGA TGGACAAAGA 
GGAGCTCCTG GTTCTCGAGG TTTAAGGGGA ATCAAAGGTG AACAAGGGGA ATGCAGATGC 
TATGAAGGAG ATGAAGCTGG CAGAGGCCCT GCTGGAGAGC CTGGTCCAGT TGGTTTTCCA 
GGTTTTCAGG GTCACGCAGG CAGAAAGGGA GAAGCAGGAG ATAGAGGAGT ACAAGGGCTT 
CCAGGAGCAC CAGGCACAGT GGGTTTAGCT GGTCCTGCTG GGTTTCCTGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTATT TGCTACAGAA AAAGGTTCCA AGGGAGCACA AGGAGACTCT 
GGTTTTCCTG GTGTTCCTGG GCGTGATGGA TACCCTGGAA GAAATGGTAG AGATGGTAGT 
CCTGGCTTTC CAGGCCCTTC TGGAGATGGT ATAAAAGGAC TCCCAGGTTC TCATGGGTTT 
CCAGGACTCC CTGGTAATGC TGGTGCTCGA GGTGAGACGG GCTTTCCTGG ACCAGGATTT 
CCAGGTTCAA AAGGGCTGCG GGGAGAGCCA GGAAACAGAG GAGTGAGCGG ATTTCCAGGA 
GTTCCTGGAC TTTCAGGCCC CCAAGGTGAC TGCTATCAAG AAACTGATGA AGAAAATGAA 
ATAGGGAAAG AAGGGACTGG CTGCAGACTA ATTGCACCTC CTGCTCCAGC AGGTGCCCCA 
GGGCCACCAG GATTACCAGG ATATAACGGT ATAAGAGGCC AGAAAGGTTT TCCCGGACCA 
CATGGTTTTG ATGGCGTTTT TGGATTAAAA GGCATCAAAG GAGACCGTGG AACCGATAGT 
TTGCCAGGTC CACCAGGTTT TTCTGGACAA AGGGGAGACA GAGGTAATGC AGGACTACCT 
GGCCTACCCG GGGTACCAGG TTTGTCAGGA AAACCTGGCT TTAAAGGGGT ACAAGGAGAA 
AAAGGAAGTG TAGGTGATCG CTTAGGAGCT CCATCAGGGG AGCAAGGAAA TACTGGCCTG 
CCTGGCTTGC AAGGACTTAA AGGAACACAA GGGGATCAAG GCATACCTGG CCTACAAGGT 
AAAGTTGGAA CACCAGGAAC ACCTGGAAAC AAGGGTATTA AAGGCTCTCC CGGGGCCCCA 
GGAGTACCTG GAATACCAGG ACCTGCAGCA TTATTTGGAT TTCCAGGAAT GCCCGGAAAC 
CCAGGCACAC CAGGACCCCA AGGTTCAATG GGACCTCCAG GTCCTCCAGG ACAGAGAGGA 
GATGAAGGCG AGAAAGGGCC ACCAGGTCCA GCTGGGATGA AAGGGTTACC TGGAGAACTT 
GGTGTTGGAG GATTTATTGG TGTTAGGGGC AGACATGGAA ATCCAGGCAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGCATCAAAG GTTCCCCTGG CATGGAAGGC 
TTCCAGGGAA TGAAAGGAGC GAAGGGGAGA CCTGCATGGA GAGGACTCAA AGGACCAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG GGAAATGGAA CTTCCGGTAC AAAAGGTGAC 
CGTGGGGAAC AAGGACCACC AGGAGACCCC CCCAAAATGC CAGAAATGAT GCTTTTGACA 
AAAGGCGAAA CAGGAGATCA AGGTGTCTCG GGCTTTAAAG GAATTTCTGG ACTTAAAGGC 
AGTAAAGGTA TGCCTGGACC TCCAGGACAG CTAGGCTTAC CTGGCTTGCC TGGACTACGA 
AGCTTTGAAT TTGGAGATAA AGGAGAAACT GGTACTCCTG GGGTTATCGG TAATCAAGGA 
ATTCTAGGAG AAGTTGGTCC AGACGGAATT ATTGGCTTCC CAGGATTTAC TGGTCCCAGA 
GGTACTCCTG GTATTTCTGG CTTTCCTGGT GTTCCTGGTG AAAGAGGCAA ATATGGAGAT 
ATAGGAGAAA GAGGTGATAC TATTGATTTA CCAGGAGAAA GAGGAACAAA GGGTCAGGGT 
GGAACCCCCG GGCTGCCAGG ACAGCGAGGA ATACACGGAG AAAAAGGAAC TACAGGAGAT 
GAAGGTTTTC GGGGTATTGA GGGAGTGATA GGGGAACACG GACAAACAGG AGAAAAAGGA 
TTCCCAGGTC AACAAGGCCT TGTGGGCTTT CCAGGCTCAC AGGGTTTTCC AGGTCTGCCA 
GGTGTGCCTG GCGAGAAAGG AAGTTCTGGG TTTCCTGGGT TACCTGGTCA ACATGGTTTT 
CCTGGCATAA GAGGGATTGC TGGCCTAGAT GGTCTGCCAG GCACTAAAGG AATTAATGGA 
CAGCCAGGTG CGGATATAAT TGGTTTAAAG GGATTTAAAG GTCTAAGTGG TGGAAAGGGT 
CAACCAGGAG AGGCTAGTAC CATTGTAGGA ATGCCTGGAC CTGCAGGATC TAAAGGAGCC 
GAAGGGGATC AAGGTTTACC TGGCTTAATT GGACTTCCTG GAACAGTTGG AGTCCGTGGC 
TTGCCTGGGT TTTCTAACAA GACAGGTTTG CTAGGAGATG TTGGACCTCC AGGACCTCCT 
GGACTGCCAG GTTTTCCAGG CCCTATCGGA AGACCAGGTT TACCCTCTAA ACCTGGAGTA 
AAAGGAGTCA TAGGGGATCT GGGGTTACAA GGGAACTATG GTGCTAAAGG TATTCCTGGT 
GACGAAGGAA AAGTGGGAGT TCCTGGACTG GCTGGAATAC CTGGAGCCAA AGGTTTGAAA 
GGCAGTGCTG GCTTCCAGGG TTTTGTTGGG GTATCAGGGT ATCGAGGAGA TCAAGGACCT 
AAAGGGCTTA GAGGTGACAT CGGAGAATAT GGTTTAAAGG GTCCTCCTGG ACAACCGGGC 
CCTATGTCTG AACCTCCTCT TATTACGGCT GAACAAGGCT ACCCTGGAGC ACACGGAATT 
ATTGGCCATC AAGGTGTTCA GGGCGATATG GGCCCAAAAG GTCTTGTGGG TACTCCAGGT 
GCCCCTGGCC AAACCGGTAA AGAAGGCTTA CCAGGTGTAC CAGGCTGGCC TGCTCCTCCA 
GGCAGTCGTG GAGAACAAGG ACCCATGGGA CGAACAGGAC CTAATGGATT CAAAGGTGCC 
AGTGGTGTGC CAGGCAGGGC AGGGCTTCCT GGGATGCCCG GGAGAAGTGT CAATATTGGC 
TATCTTTTAG TCAAGCACAG TCAAACAGAT GAAGAGCCAA TGTGTCCTGT TGGTATGGCC 
CGCTTGTGGA CTGGGTATAG TCTCTTGTAT TTTGAAGGAC AGGAAAAAGC ACACAATCAA 
GACTTAGGTC TAGCTGGATC TTGTTTACAG CGCTTCAGTA CAATGCCGTT CCTGTACTGC 
AACCCAGGGG ATGTCTGTTA TTATGCCAAC CGTAACGATA AGTCTTATTG GCTTTCCACC 
ACTGCACCTC TTCCAATGAT GCCTGTGGTT GAAGAAGAAA TTAGGCCATA TATTAGCCGT 
TGCTCAGTGT GTGAGGCTCC AGCAGTAGCA ATTGCTGTTC ACAGTCAAGA TGTCAGTATC 
CCACATTGCC CAGATGGTTG GCGGAGCTTG TGGATTGGCT ACTCCTTTCT CATGCACACT 
GCCGCAGGCG ATGAGGGGGG AGGACAATCT CTCTCCTCCC CTGGAAGTTG TTTGGAAGAC 
TTCAGGGCGA CTCCATTCAT TGAATGCAAT GGTGGAAGAG GGACCTGCCA CTACTTTGCA 
AACAAATACA GTTTCTGGCT TACTACTATT GACGAGCCCT TCCAGAGCTC TCCGCCTGCA 
GATACACTAA AAGCTGGGCT GATTCGAACT CATATCAGCC GATGCCAAGT CTGCATGAAA 
AATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

col4a2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報