COL4A2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL4A2
Entrez Gene ID	1284
Full Name	collagen type IV alpha 2 chain
Synonyms	ICH,POREN2
General protein information	Preferred Names collagen type IV alpha 2 chain Names collagen alpha-2(IV) chain canstatin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q34
Summary	This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jul 2008].
Disorder MIM:	120090
Disorder Html:	Porencephaly 2, 614483 (3); {Hemorrhage, intracerebral, susceptibility to}, 614519 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001846.4	NP_001837.2	collagen alpha-2(IV) chain preproprotein
NM_001846.3	NP_001837.2	collagen alpha-2(IV) chain preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL4A2	XP_001136859.1
Canis lupus familiaris (dog)	COL4A2	XP_534183.3
Rattus norvegicus (Norway rat)	Col4a2	XP_006253538.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009200	XP_319976.3
	COL4A2	XP_002800878.1
Drosophila melanogaster (fruit fly)	Cg25C	NP_723046.1
Caenorhabditis elegans (roundworm)	emb-9	NP_001022662.1
Bos taurus (cattle)	COL4A2	XP_002692057.2
Mus musculus (house mouse)	Col4a2	NP_034062.3
Gallus gallus (chicken)	COL4A2	NP_001155862.1
Danio rerio (zebrafish)	col4a2	XP_687811.6
Caenorhabditis elegans (roundworm)	let-2	NP_510663.1
Xenopus tropicalis (tropical clawed frog)	col4a2	XP_004912045.1
Homo sapiens (human)	COL4A2	NP_001837.2

Gene Ontology
Bibliography

Related articles in PubMed

Rannikm?e K, Sivakumaran V, Millar H, Malik R, Anderson CD, Chong M, Dave T, Falcone GJ, Fernandez-Cadenas I, Jimenez-Conde J, Lindgren A, Montaner J, O'Donnell M, Par? G, Radmanesh F, Rost NS, Slowik A, S?derholm M, Traylor M, Pulit SL, Seshadri S, Worrall BB, Woo D, Markus HS, Mitchell BD, Dichgans M, Rosand J, Sudlow CLM,
Neurology89(17)1829-1839(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results of this study provide evidence that the mutation of COL4A2 is associated with lacunar ischemic stroke and deep intracerebral hemorrhage.

COL4A2 expression is significantly upregulated in human masticatory mucosa during wound healing
Title: Human gingiva transcriptome during wound healing.

Genotype-phenotype correlations in pathology caused by COL4A1 and COL4A2 mutations have been summarized. (Review)
Title: Genotype-phenotype correlations in pathology caused by collagen type IV alpha 1 and 2 mutations.

Expression of miR-26a and miR-29a was significantly down regulated in leukoplakia and cancer tissues but up regulated in lichen planus tissues. Expression of target genes such as, ADAMTS7, ATP1B1, COL4A2, CPEB3, CDK6, DNMT3a and PI3KR1 was significantly down regulated in at least two of three disease types with respect to normal tissues.
Title: MicroRNA and target gene expression based clustering of oral cancer, precancer and normal tissues.

In this study, the inhibitory effect of recombinant canstatin on tumour growth was investigated using a gastric cancer xenograft model.
Title: Canstatin induces apoptosis in gastric cancer xenograft growth in mice through the mitochondrial apoptotic pathway.

The following COL4A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu108585	NM_001846.4 Latest version!	Homo sapiens collagen type IV alpha 2 chain (COL4A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu19483	NM_001846.3 Latest version!	Homo sapiens collagen type IV alpha 2 chain (COL4A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL4A2 ( NM_001846.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001837.2 Homo sapiens collagen type IV alpha 2 chain (COL4A2), mRNA.

Price & Availability↑

CloneID	OHu108585
Clone ID Related Accession (Same CDS sequence)	NM_001846.4
Accession Version	NM_001846.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	collagen alpha-2(IV) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161773.21, AL159153.17 and AL139385.12. On May 17, 2019 this sequence version replaced NM_001846.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000360467.7/ ENSP00000353654.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGAGAG ACCAGCGCGC GGTGGCCGGC CCTGCCCTAC GGCGGTGGCT GCTGCTGGGG 
ACAGTGACCG TGGGGTTCCT CGCCCAGAGC GTCTTGGCGG GTGTGAAGAA GTTTGATGTG 
CCGTGTGGAG GAAGAGATTG CAGTGGGGGC TGCCAGTGCT ACCCTGAGAA AGGTGGACGT 
GGTCAGCCTG GGCCAGTGGG CCCCCAGGGG TACAATGGGC CACCAGGATT ACAAGGATTC 
CCGGGACTGC AGGGACGTAA AGGAGACAAG GGTGAAAGGG GAGCCCCCGG AGTAACGGGA 
CCCAAGGGCG ACGTGGGAGC AAGAGGCGTT TCTGGATTCC CTGGTGCCGA TGGAATTCCT 
GGACACCCGG GGCAAGGTGG GCCCAGGGGA AGGCCGGGCT ACGATGGCTG CAACGGAACC 
CAGGGAGACT CAGGTCCACA GGGGCCCCCC GGCTCTGAGG GGTTCACCGG GCCTCCCGGG 
CCCCAAGGAC CAAAAGGGCA GAAAGGTGAG CCTTATGCAC TGCCTAAAGA GGAGCGCGAC 
AGATATCGGG GTGAACCTGG AGAGCCTGGA TTGGTCGGTT TCCAGGGACC TCCCGGCCGC 
CCTGGGCATG TGGGACAGAT GGGTCCAGTT GGAGCTCCAG GGAGACCAGG ACCACCTGGA 
CCCCCTGGAC CAAAAGGACA GCAAGGCAAC AGAGGACTTG GTTTCTACGG AGTTAAGGGT 
GAAAAGGGTG ACGTAGGGCA GCCGGGACCC AACGGGATTC CATCAGACAC CCTCCACCCC 
ATCATCGCGC CCACAGGAGT CACCTTCCAC CCAGATCAGT ACAAGGGTGA AAAAGGCAGT 
GAGGGGGAAC CAGGAATAAG AGGCATTTCC TTGAAGGGAG AAGAAGGAAT CATGGGCTTT 
CCTGGACTGA GGGGTTACCC TGGCTTGAGT GGTGAAAAAG GATCACCAGG ACAGAAGGGA 
AGCCGAGGCC TGGATGGCTA TCAAGGGCCT GATGGACCCC GGGGACCCAA GGGAGAAGCC 
GGAGACCCAG GGCCCCCTGG ACTACCTGCC TACTCCCCTC ACCCTTCCCT AGCAAAAGGT 
GCCAGAGGTG ACCCGGGATT CCCAGGGGCC CAAGGGGAGC CAGGAAGCCA GGGTGAGCCA 
GGAGACCCGG GCCTCCCAGG TCCCCCTGGC CTCTCCATCG GAGATGGAGA TCAGAGGAGA 
GGCCTGCCGG GTGAGATGGG ACCCAAGGGC TTCATCGGAG ACCCCGGCAT CCCTGCGCTC 
TACGGGGGCC CACCTGGACC TGATGGAAAG CGAGGGCCTC CAGGACCCCC CGGGCTCCCT 
GGACCACCTG GACCTGATGG CTTCCTGTTT GGGCTGAAAG GAGCAAAAGG AAGAGCAGGC 
TTCCCTGGGC TTCCCGGCTC CCCTGGAGCC CGCGGACCAA AGGGGTGGAA AGGTGACGCT 
GGGGAATGCA GATGTACAGA AGGCGACGAA GCTATCAAAG GTCTTCCGGG ACTGCCAGGA 
CCCAAGGGCT TCGCAGGCAT CAACGGGGAG CCGGGGAGGA AAGGGGACAG AGGAGACCCC 
GGCCAACACG GCCTCCCTGG GTTCCCAGGG CTCAAGGGAG TGCCTGGCAA CATTGGTGCT 
CCCGGACCCA AAGGAGCAAA AGGAGATTCC AGAACAATCA CAACCAAAGG TGAGCGGGGA 
CAGCCCGGCG TCCCAGGTGT GCCCGGGATG AAAGGTGACG ATGGCAGCCC AGGCCGCGAT 
GGGCTCGATG GATTCCCCGG CCTCCCAGGC CCTCCCGGTG ATGGCATCAA GGGCCCTCCA 
GGGGACCCAG GCTATCCAGG AATACCTGGA ACGAAGGGTA CTCCAGGAGA AATGGGCCCC 
CCAGGACTGG GCCTTCCCGG CCTCAAAGGC CAACGTGGTT TCCCTGGAGA CGCCGGCTTA 
CCTGGACCAC CAGGCTTCCT GGGCCCTCCT GGCCCCGCAG GGACCCCAGG ACAAATAGAT 
TGTGACACAG ATGTGAAAAG GGCCGTTGGA GGTGACAGAC AGGAGGCCAT CCAGCCAGGT 
TGCATAGGAG GGCCCAAGGG ATTGCCAGGC CTGCCAGGAC CCCCAGGCCC CACAGGTGCC 
AAAGGCCTCC GAGGAATCCC AGGCTTCGCA GGAGCTGATG GAGGACCAGG GCCCAGGGGC 
TTGCCAGGAG ACGCAGGTCG TGAAGGGTTC CCAGGACCCC CAGGGTTCAT AGGACCCCGA 
GGATCCAAAG GTGCAGTGGG CCTCCCTGGC CCAGATGGAT CCCCAGGTCC CATCGGCCTG 
CCAGGGCCAG ATGGGCCCCC TGGGGAAAGG GGCCTCCCTG GAGAAGTCCT GGGAGCTCAG 
CCCGGGCCAC GGGGAGATGC TGGTGTGCCT GGACAGCCTG GGCTTAAAGG CCTTCCCGGA 
GACAGAGGCC CCCCTGGATT CAGAGGAAGC CAAGGGATGC CTGGGATGCC AGGGCTGAAG 
GGCCAGCCAG GCCTCCCAGG ACCTTCCGGC CAGCCAGGCC TGTATGGGCC TCCAGGACTG 
CATGGATTCC CAGGAGCTCC TGGCCAAGAG GGGCCCTTGG GGCTGCCAGG AATCCCAGGC 
CGTGAAGGTC TGCCTGGTGA TAGAGGGGAC CCTGGGGACA CAGGCGCTCC TGGCCCTGTG 
GGCATGAAAG GTCTCTCTGG TGACAGAGGA GATGCTGGCT TCACAGGGGA GCAAGGCCAT 
CCAGGAAGCC CTGGATTTAA AGGAATTGAT GGAATGCCTG GGACCCCCGG GCTAAAAGGA 
GATAGAGGCT CACCTGGGAT GGATGGTTTC CAAGGCATGC CTGGACTCAA AGGGAGACCC 
GGGTTTCCAG GGAGCAAAGG CGAGGCTGGA TTTTTCGGAA TACCCGGTCT GAAGGGTCTG 
GCTGGTGAGC CAGGTTTTAA AGGCAGCCGA GGGGACCCTG GGCCCCCAGG ACCACCTCCT 
GTCATCCTGC CAGGAATGAA AGACATTAAA GGAGAGAAAG GAGATGAAGG GCCTATGGGG 
CTGAAAGGAT ACCTGGGCGC AAAAGGTATC CAAGGAATGC CAGGCATCCC AGGGCTGTCA 
GGAATCCCTG GGCTGCCTGG GAGGCCCGGC CACATCAAAG GAGTCAAGGG AGACATCGGA 
GTCCCCGGCA TCCCCGGTTT GCCAGGATTC CCTGGGGTGG CTGGCCCCCC TGGAATTACG 
GGATTCCCAG GATTCATAGG AAGCCGGGGT GACAAAGGTG CCCCAGGGAG AGCAGGCCTG 
TATGGCGAGA TTGGCGCGAC TGGTGATTTC GGTGACATCG GGGACACTAT AAATTTACCA 
GGAAGACCAG GCCTGAAGGG GGAGCGGGGC ACCACTGGAA TACCAGGTCT GAAGGGATTC 
TTTGGAGAGA AGGGAACAGA AGGTGACATC GGCTTCCCTG GGATAACAGG CGTGACTGGA 
GTCCAAGGCC CTCCTGGACT TAAAGGACAA ACAGGCTTTC CAGGGCTGAC TGGGCCTCCA 
GGGTCGCAGG GAGAGCTGGG GCGGATTGGA CTGCCTGGTG GCAAAGGAGA TGATGGCTGG 
CCGGGAGCTC CGGGCTTACC AGGTTTTCCG GGACTCCGTG GGATCCGCGG CTTACACGGC 
TTGCCAGGCA CCAAGGGCTT TCCAGGATCC CCAGGTTCTG ACATCCACGG AGACCCAGGC 
TTCCCAGGCC CTCCTGGGGA AAGAGGTGAC CCAGGAGAGG CCAACACCCT TCCAGGCCCT 
GTGGGAGTCC CAGGACAGAA AGGAGACCAA GGAGCTCCAG GGGAACGAGG CCCACCTGGG 
AGCCCAGGAC TTCAGGGGTT CCCTGGTATC ACACCCCCTT CCAACATCTC TGGGGCACCT 
GGTGACAAAG GGGCGCCAGG GATATTTGGC CTGAAAGGTT ATCGGGGCCC ACCAGGGCCA 
CCAGGTTCTG CTGCTCTTCC TGGAAGCAAA GGTGACACAG GGAACCCAGG AGCTCCAGGA 
ACCCCAGGGA CCAAAGGATG GGCCGGGGAC TCCGGGCCCC AGGGCAGGCC TGGTGTGTTT 
GGTCTCCCAG GAGAAAAAGG GCCCAGGGGT GAACAAGGCT TCATGGGGAA CACTGGACCC 
ACTGGGGCGG TGGGCGACAG AGGCCCCAAG GGACCCAAGG GAGACCCAGG ATTCCCTGGT 
GCCCCCGGGA CTGTGGGAGC CCCCGGGATT GCAGGAATCC CCCAGAAGAT TGCCGTCCAA 
CCAGGGACAG TGGGTCCCCA GGGGAGGCGA GGCCCCCCTG GGGCACCGGG GGAGATGGGG 
CCCCAGGGCC CCCCCGGAGA ACCAGGTTTC CGTGGGGCTC CAGGGAAAGC TGGGCCCCAA 
GGAAGAGGTG GTGTGTCTGC TGTTCCCGGC TTCCGGGGAG ATGAAGGACC CATAGGCCAC 
CAGGGGCCGA TTGGCCAAGA AGGTGCACCA GGCCGTCCAG GGAGCCCGGG CCTGCCGGGT 
ATGCCAGGCC GCAGCGTCAG CATCGGCTAC CTCCTGGTGA AGCACAGCCA GACGGACCAG 
GAGCCCATGT GCCCAGTGGG CATGAACAAA CTCTGGAGTG GATACAGCCT GCTGTACTTC 
GAGGGCCAGG AGAAGGCGCA CAACCAGGAC CTGGGGCTGG CGGGCTCCTG CCTGGCGCGG 
TTCAGCACCA TGCCCTTCCT GTACTGCAAC CCTGGTGATG TCTGCTACTA TGCCAGCCGG 
AACGACAAGT CCTACTGGCT CTCTACCACT GCGCCGCTGC CCATGATGCC CGTGGCCGAG 
GACGAGATCA AGCCCTACAT CAGCCGCTGT TCTGTGTGTG AGGCCCCGGC CATCGCCATC 
GCGGTCCACA GTCAGGATGT CTCCATCCCA CACTGCCCAG CTGGGTGGCG GAGTTTGTGG 
ATCGGATATT CCTTCCTCAT GCACACGGCG GCGGGAGACG AAGGCGGTGG CCAATCACTG 
GTGTCACCGG GCAGCTGTCT AGAGGACTTC CGCGCCACAC CATTCATCGA ATGCAATGGA 
GGCCGCGGCA CCTGCCACTA CTACGCCAAC AAGTACAGCT TCTGGCTGAC CACCATTCCC 
GAGCAGAGCT TCCAGGGCTC GCCCTCCGCC GACACGCTCA AGGCCGGCCT CATCCGCACA 
CACATCAGCC GCTGCCAGGT GTGCATGAAG AACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001837.2
CDS	290..5428
Translation	MGRDQRAVAGPALRRWLLLGTVTVGFLAQSVLAGVKKFDVPCGGRDCSGGCQCYPEKGGRGQPGPVGPQGYNGPPGLQGFPGLQGRKGDKGERGAPGVTGPKGDVGARGVSGFPGADGIPGHPGQGGPRGRPGYDGCNGTQGDSGPQGPPGSEGFTGPPGPQGPKGQKGEPYALPKEERDRYRGEPGEPGLVGFQGPPGRPGHVGQMGPVGAPGRPGPPGPPGPKGQQGNRGLGFYGVKGEKGDVGQPGPNGIPSDTLHPIIAPTGVTFHPDQYKGEKGSEGEPGIRGISLKGEEGIMGFPGLRGYPGLSGEKGSPGQKGSRGLDGYQGPDGPRGPKGEAGDPGPPGLPAYSPHPSLAKGARGDPGFPGAQGEPGSQGEPGDPGLPGPPGLSIGDGDQRRGLPGEMGPKGFIGDPGIPALYGGPPGPDGKRGPPGPPGLPGPPGPDGFLFGLKGAKGRAGFPGLPGSPGARGPKGWKGDAGECRCTEGDEAIKGLPGLPGPKGFAGINGEPGRKGDRGDPGQHGLPGFPGLKGVPGNIGAPGPKGAKGDSRTITTKGERGQPGVPGVPGMKGDDGSPGRDGLDGFPGLPGPPGDGIKGPPGDPGYPGIPGTKGTPGEMGPPGLGLPGLKGQRGFPGDAGLPGPPGFLGPPGPAGTPGQIDCDTDVKRAVGGDRQEAIQPGCIGGPKGLPGLPGPPGPTGAKGLRGIPGFAGADGGPGPRGLPGDAGREGFPGPPGFIGPRGSKGAVGLPGPDGSPGPIGLPGPDGPPGERGLPGEVLGAQPGPRGDAGVPGQPGLKGLPGDRGPPGFRGSQGMPGMPGLKGQPGLPGPSGQPGLYGPPGLHGFPGAPGQEGPLGLPGIPGREGLPGDRGDPGDTGAPGPVGMKGLSGDRGDAGFTGEQGHPGSPGFKGIDGMPGTPGLKGDRGSPGMDGFQGMPGLKGRPGFPGSKGEAGFFGIPGLKGLAGEPGFKGSRGDPGPPGPPPVILPGMKDIKGEKGDEGPMGLKGYLGAKGIQGMPGIPGLSGIPGLPGRPGHIKGVKGDIGVPGIPGLPGFPGVAGPPGITGFPGFIGSRGDKGAPGRAGLYGEIGATGDFGDIGDTINLPGRPGLKGERGTTGIPGLKGFFGEKGTEGDIGFPGITGVTGVQGPPGLKGQTGFPGLTGPPGSQGELGRIGLPGGKGDDGWPGAPGLPGFPGLRGIRGLHGLPGTKGFPGSPGSDIHGDPGFPGPPGERGDPGEANTLPGPVGVPGQKGDQGAPGERGPPGSPGLQGFPGITPPSNISGAPGDKGAPGIFGLKGYRGPPGPPGSAALPGSKGDTGNPGAPGTPGTKGWAGDSGPQGRPGVFGLPGEKGPRGEQGFMGNTGPTGAVGDRGPKGPKGDPGFPGAPGTVGAPGIAGIPQKIAVQPGTVGPQGRRGPPGAPGEMGPQGPPGEPGFRGAPGKAGPQGRGGVSAVPGFRGDEGPIGHQGPIGQEGAPGRPGSPGLPGMPGRSVSIGYLLVKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEDEIKPYISRCSVCEAPAIAIAVHSQDVSIPHCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYYANKYSFWLTTIPEQSFQGSPSADTLKAGLIRTHISRCQVCMKNL

Target ORF information:

RefSeq Version	NM_001846.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 2 chain (COL4A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001846.4

ORF Insert Sequence:

ATGGGGAGAG ACCAGCGCGC GGTGGCCGGC CCTGCCCTAC GGCGGTGGCT GCTGCTGGGG 
ACAGTGACCG TGGGGTTCCT CGCCCAGAGC GTCTTGGCGG GTGTGAAGAA GTTTGATGTG 
CCGTGTGGAG GAAGAGATTG CAGTGGGGGC TGCCAGTGCT ACCCTGAGAA AGGTGGACGT 
GGTCAGCCTG GGCCAGTGGG CCCCCAGGGG TACAATGGGC CACCAGGATT ACAAGGATTC 
CCGGGACTGC AGGGACGTAA AGGAGACAAG GGTGAAAGGG GAGCCCCCGG AGTAACGGGA 
CCCAAGGGCG ACGTGGGAGC AAGAGGCGTT TCTGGATTCC CTGGTGCCGA TGGAATTCCT 
GGACACCCGG GGCAAGGTGG GCCCAGGGGA AGGCCGGGCT ACGATGGCTG CAACGGAACC 
CAGGGAGACT CAGGTCCACA GGGGCCCCCC GGCTCTGAGG GGTTCACCGG GCCTCCCGGG 
CCCCAAGGAC CAAAAGGGCA GAAAGGTGAG CCTTATGCAC TGCCTAAAGA GGAGCGCGAC 
AGATATCGGG GTGAACCTGG AGAGCCTGGA TTGGTCGGTT TCCAGGGACC TCCCGGCCGC 
CCTGGGCATG TGGGACAGAT GGGTCCAGTT GGAGCTCCAG GGAGACCAGG ACCACCTGGA 
CCCCCTGGAC CAAAAGGACA GCAAGGCAAC AGAGGACTTG GTTTCTACGG AGTTAAGGGT 
GAAAAGGGTG ACGTAGGGCA GCCGGGACCC AACGGGATTC CATCAGACAC CCTCCACCCC 
ATCATCGCGC CCACAGGAGT CACCTTCCAC CCAGATCAGT ACAAGGGTGA AAAAGGCAGT 
GAGGGGGAAC CAGGAATAAG AGGCATTTCC TTGAAGGGAG AAGAAGGAAT CATGGGCTTT 
CCTGGACTGA GGGGTTACCC TGGCTTGAGT GGTGAAAAAG GATCACCAGG ACAGAAGGGA 
AGCCGAGGCC TGGATGGCTA TCAAGGGCCT GATGGACCCC GGGGACCCAA GGGAGAAGCC 
GGAGACCCAG GGCCCCCTGG ACTACCTGCC TACTCCCCTC ACCCTTCCCT AGCAAAAGGT 
GCCAGAGGTG ACCCGGGATT CCCAGGGGCC CAAGGGGAGC CAGGAAGCCA GGGTGAGCCA 
GGAGACCCGG GCCTCCCAGG TCCCCCTGGC CTCTCCATCG GAGATGGAGA TCAGAGGAGA 
GGCCTGCCGG GTGAGATGGG ACCCAAGGGC TTCATCGGAG ACCCCGGCAT CCCTGCGCTC 
TACGGGGGCC CACCTGGACC TGATGGAAAG CGAGGGCCTC CAGGACCCCC CGGGCTCCCT 
GGACCACCTG GACCTGATGG CTTCCTGTTT GGGCTGAAAG GAGCAAAAGG AAGAGCAGGC 
TTCCCTGGGC TTCCCGGCTC CCCTGGAGCC CGCGGACCAA AGGGGTGGAA AGGTGACGCT 
GGGGAATGCA GATGTACAGA AGGCGACGAA GCTATCAAAG GTCTTCCGGG ACTGCCAGGA 
CCCAAGGGCT TCGCAGGCAT CAACGGGGAG CCGGGGAGGA AAGGGGACAG AGGAGACCCC 
GGCCAACACG GCCTCCCTGG GTTCCCAGGG CTCAAGGGAG TGCCTGGCAA CATTGGTGCT 
CCCGGACCCA AAGGAGCAAA AGGAGATTCC AGAACAATCA CAACCAAAGG TGAGCGGGGA 
CAGCCCGGCG TCCCAGGTGT GCCCGGGATG AAAGGTGACG ATGGCAGCCC AGGCCGCGAT 
GGGCTCGATG GATTCCCCGG CCTCCCAGGC CCTCCCGGTG ATGGCATCAA GGGCCCTCCA 
GGGGACCCAG GCTATCCAGG AATACCTGGA ACGAAGGGTA CTCCAGGAGA AATGGGCCCC 
CCAGGACTGG GCCTTCCCGG CCTCAAAGGC CAACGTGGTT TCCCTGGAGA CGCCGGCTTA 
CCTGGACCAC CAGGCTTCCT GGGCCCTCCT GGCCCCGCAG GGACCCCAGG ACAAATAGAT 
TGTGACACAG ATGTGAAAAG GGCCGTTGGA GGTGACAGAC AGGAGGCCAT CCAGCCAGGT 
TGCATAGGAG GGCCCAAGGG ATTGCCAGGC CTGCCAGGAC CCCCAGGCCC CACAGGTGCC 
AAAGGCCTCC GAGGAATCCC AGGCTTCGCA GGAGCTGATG GAGGACCAGG GCCCAGGGGC 
TTGCCAGGAG ACGCAGGTCG TGAAGGGTTC CCAGGACCCC CAGGGTTCAT AGGACCCCGA 
GGATCCAAAG GTGCAGTGGG CCTCCCTGGC CCAGATGGAT CCCCAGGTCC CATCGGCCTG 
CCAGGGCCAG ATGGGCCCCC TGGGGAAAGG GGCCTCCCTG GAGAAGTCCT GGGAGCTCAG 
CCCGGGCCAC GGGGAGATGC TGGTGTGCCT GGACAGCCTG GGCTTAAAGG CCTTCCCGGA 
GACAGAGGCC CCCCTGGATT CAGAGGAAGC CAAGGGATGC CTGGGATGCC AGGGCTGAAG 
GGCCAGCCAG GCCTCCCAGG ACCTTCCGGC CAGCCAGGCC TGTATGGGCC TCCAGGACTG 
CATGGATTCC CAGGAGCTCC TGGCCAAGAG GGGCCCTTGG GGCTGCCAGG AATCCCAGGC 
CGTGAAGGTC TGCCTGGTGA TAGAGGGGAC CCTGGGGACA CAGGCGCTCC TGGCCCTGTG 
GGCATGAAAG GTCTCTCTGG TGACAGAGGA GATGCTGGCT TCACAGGGGA GCAAGGCCAT 
CCAGGAAGCC CTGGATTTAA AGGAATTGAT GGAATGCCTG GGACCCCCGG GCTAAAAGGA 
GATAGAGGCT CACCTGGGAT GGATGGTTTC CAAGGCATGC CTGGACTCAA AGGGAGACCC 
GGGTTTCCAG GGAGCAAAGG CGAGGCTGGA TTTTTCGGAA TACCCGGTCT GAAGGGTCTG 
GCTGGTGAGC CAGGTTTTAA AGGCAGCCGA GGGGACCCTG GGCCCCCAGG ACCACCTCCT 
GTCATCCTGC CAGGAATGAA AGACATTAAA GGAGAGAAAG GAGATGAAGG GCCTATGGGG 
CTGAAAGGAT ACCTGGGCGC AAAAGGTATC CAAGGAATGC CAGGCATCCC AGGGCTGTCA 
GGAATCCCTG GGCTGCCTGG GAGGCCCGGC CACATCAAAG GAGTCAAGGG AGACATCGGA 
GTCCCCGGCA TCCCCGGTTT GCCAGGATTC CCTGGGGTGG CTGGCCCCCC TGGAATTACG 
GGATTCCCAG GATTCATAGG AAGCCGGGGT GACAAAGGTG CCCCAGGGAG AGCAGGCCTG 
TATGGCGAGA TTGGCGCGAC TGGTGATTTC GGTGACATCG GGGACACTAT AAATTTACCA 
GGAAGACCAG GCCTGAAGGG GGAGCGGGGC ACCACTGGAA TACCAGGTCT GAAGGGATTC 
TTTGGAGAGA AGGGAACAGA AGGTGACATC GGCTTCCCTG GGATAACAGG CGTGACTGGA 
GTCCAAGGCC CTCCTGGACT TAAAGGACAA ACAGGCTTTC CAGGGCTGAC TGGGCCTCCA 
GGGTCGCAGG GAGAGCTGGG GCGGATTGGA CTGCCTGGTG GCAAAGGAGA TGATGGCTGG 
CCGGGAGCTC CGGGCTTACC AGGTTTTCCG GGACTCCGTG GGATCCGCGG CTTACACGGC 
TTGCCAGGCA CCAAGGGCTT TCCAGGATCC CCAGGTTCTG ACATCCACGG AGACCCAGGC 
TTCCCAGGCC CTCCTGGGGA AAGAGGTGAC CCAGGAGAGG CCAACACCCT TCCAGGCCCT 
GTGGGAGTCC CAGGACAGAA AGGAGACCAA GGAGCTCCAG GGGAACGAGG CCCACCTGGG 
AGCCCAGGAC TTCAGGGGTT CCCTGGTATC ACACCCCCTT CCAACATCTC TGGGGCACCT 
GGTGACAAAG GGGCGCCAGG GATATTTGGC CTGAAAGGTT ATCGGGGCCC ACCAGGGCCA 
CCAGGTTCTG CTGCTCTTCC TGGAAGCAAA GGTGACACAG GGAACCCAGG AGCTCCAGGA 
ACCCCAGGGA CCAAAGGATG GGCCGGGGAC TCCGGGCCCC AGGGCAGGCC TGGTGTGTTT 
GGTCTCCCAG GAGAAAAAGG GCCCAGGGGT GAACAAGGCT TCATGGGGAA CACTGGACCC 
ACTGGGGCGG TGGGCGACAG AGGCCCCAAG GGACCCAAGG GAGACCCAGG ATTCCCTGGT 
GCCCCCGGGA CTGTGGGAGC CCCCGGGATT GCAGGAATCC CCCAGAAGAT TGCCGTCCAA 
CCAGGGACAG TGGGTCCCCA GGGGAGGCGA GGCCCCCCTG GGGCACCGGG GGAGATGGGG 
CCCCAGGGCC CCCCCGGAGA ACCAGGTTTC CGTGGGGCTC CAGGGAAAGC TGGGCCCCAA 
GGAAGAGGTG GTGTGTCTGC TGTTCCCGGC TTCCGGGGAG ATGAAGGACC CATAGGCCAC 
CAGGGGCCGA TTGGCCAAGA AGGTGCACCA GGCCGTCCAG GGAGCCCGGG CCTGCCGGGT 
ATGCCAGGCC GCAGCGTCAG CATCGGCTAC CTCCTGGTGA AGCACAGCCA GACGGACCAG 
GAGCCCATGT GCCCAGTGGG CATGAACAAA CTCTGGAGTG GATACAGCCT GCTGTACTTC 
GAGGGCCAGG AGAAGGCGCA CAACCAGGAC CTGGGGCTGG CGGGCTCCTG CCTGGCGCGG 
TTCAGCACCA TGCCCTTCCT GTACTGCAAC CCTGGTGATG TCTGCTACTA TGCCAGCCGG 
AACGACAAGT CCTACTGGCT CTCTACCACT GCGCCGCTGC CCATGATGCC CGTGGCCGAG 
GACGAGATCA AGCCCTACAT CAGCCGCTGT TCTGTGTGTG AGGCCCCGGC CATCGCCATC 
GCGGTCCACA GTCAGGATGT CTCCATCCCA CACTGCCCAG CTGGGTGGCG GAGTTTGTGG 
ATCGGATATT CCTTCCTCAT GCACACGGCG GCGGGAGACG AAGGCGGTGG CCAATCACTG 
GTGTCACCGG GCAGCTGTCT AGAGGACTTC CGCGCCACAC CATTCATCGA ATGCAATGGA 
GGCCGCGGCA CCTGCCACTA CTACGCCAAC AAGTACAGCT TCTGGCTGAC CACCATTCCC 
GAGCAGAGCT TCCAGGGCTC GCCCTCCGCC GACACGCTCA AGGCCGGCCT CATCCGCACA 
CACATCAGCC GCTGCCAGGT GTGCATGAAG AACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A2 ( NM_001846.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001837.2 Homo sapiens collagen type IV alpha 2 chain (COL4A2), mRNA.

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CloneID	OHu19483
Clone ID Related Accession (Same CDS sequence)	NM_001846.3
Accession Version	NM_001846.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	collagen alpha-2(IV) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN397864.1, CB148713.1, CN397790.1, CB045156.1, CN397819.1, CK000715.1, CN397808.1, BY795218.2, BX648393.1, M24766.1, AL139385.12 and AW298730.1. This sequence is a reference standard in the RefSeqGene project. On Jun 29, 2016 this sequence version replaced NM_001846.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGAGAG ACCAGCGCGC GGTGGCCGGC CCTGCCCTAC GGCGGTGGCT GCTGCTGGGG 
ACAGTGACCG TGGGGTTCCT CGCCCAGAGC GTCTTGGCGG GTGTGAAGAA GTTTGATGTG 
CCGTGTGGAG GAAGAGATTG CAGTGGGGGC TGCCAGTGCT ACCCTGAGAA AGGTGGACGT 
GGTCAGCCTG GGCCAGTGGG CCCCCAGGGG TACAATGGGC CACCAGGATT ACAAGGATTC 
CCGGGACTGC AGGGACGTAA AGGAGACAAG GGTGAAAGGG GAGCCCCCGG AGTAACGGGA 
CCCAAGGGCG ACGTGGGAGC AAGAGGCGTT TCTGGATTCC CTGGTGCCGA TGGAATTCCT 
GGACACCCGG GGCAAGGTGG GCCCAGGGGA AGGCCGGGCT ACGATGGCTG CAACGGAACC 
CAGGGAGACT CAGGTCCACA GGGGCCCCCC GGCTCTGAGG GGTTCACCGG GCCTCCCGGG 
CCCCAAGGAC CAAAAGGGCA GAAAGGTGAG CCTTATGCAC TGCCTAAAGA GGAGCGCGAC 
AGATATCGGG GTGAACCTGG AGAGCCTGGA TTGGTCGGTT TCCAGGGACC TCCCGGCCGC 
CCTGGGCATG TGGGACAGAT GGGTCCAGTT GGAGCTCCAG GGAGACCAGG ACCACCTGGA 
CCCCCTGGAC CAAAAGGACA GCAAGGCAAC AGAGGACTTG GTTTCTACGG AGTTAAGGGT 
GAAAAGGGTG ACGTAGGGCA GCCGGGACCC AACGGGATTC CATCAGACAC CCTCCACCCC 
ATCATCGCGC CCACAGGAGT CACCTTCCAC CCAGATCAGT ACAAGGGTGA AAAAGGCAGT 
GAGGGGGAAC CAGGAATAAG AGGCATTTCC TTGAAGGGAG AAGAAGGAAT CATGGGCTTT 
CCTGGACTGA GGGGTTACCC TGGCTTGAGT GGTGAAAAAG GATCACCAGG ACAGAAGGGA 
AGCCGAGGCC TGGATGGCTA TCAAGGGCCT GATGGACCCC GGGGACCCAA GGGAGAAGCC 
GGAGACCCAG GGCCCCCTGG ACTACCTGCC TACTCCCCTC ACCCTTCCCT AGCAAAAGGT 
GCCAGAGGTG ACCCGGGATT CCCAGGGGCC CAAGGGGAGC CAGGAAGCCA GGGTGAGCCA 
GGAGACCCGG GCCTCCCAGG TCCCCCTGGC CTCTCCATCG GAGATGGAGA TCAGAGGAGA 
GGCCTGCCGG GTGAGATGGG ACCCAAGGGC TTCATCGGAG ACCCCGGCAT CCCTGCGCTC 
TACGGGGGCC CACCTGGACC TGATGGAAAG CGAGGGCCTC CAGGACCCCC CGGGCTCCCT 
GGACCACCTG GACCTGATGG CTTCCTGTTT GGGCTGAAAG GAGCAAAAGG AAGAGCAGGC 
TTCCCTGGGC TTCCCGGCTC CCCTGGAGCC CGCGGACCAA AGGGGTGGAA AGGTGACGCT 
GGGGAATGCA GATGTACAGA AGGCGACGAA GCTATCAAAG GTCTTCCGGG ACTGCCAGGA 
CCCAAGGGCT TCGCAGGCAT CAACGGGGAG CCGGGGAGGA AAGGGGACAG AGGAGACCCC 
GGCCAACACG GCCTCCCTGG GTTCCCAGGG CTCAAGGGAG TGCCTGGCAA CATTGGTGCT 
CCCGGACCCA AAGGAGCAAA AGGAGATTCC AGAACAATCA CAACCAAAGG TGAGCGGGGA 
CAGCCCGGCG TCCCAGGTGT GCCCGGGATG AAAGGTGACG ATGGCAGCCC AGGCCGCGAT 
GGGCTCGATG GATTCCCCGG CCTCCCAGGC CCTCCCGGTG ATGGCATCAA GGGCCCTCCA 
GGGGACCCAG GCTATCCAGG AATACCTGGA ACGAAGGGTA CTCCAGGAGA AATGGGCCCC 
CCAGGACTGG GCCTTCCCGG CCTCAAAGGC CAACGTGGTT TCCCTGGAGA CGCCGGCTTA 
CCTGGACCAC CAGGCTTCCT GGGCCCTCCT GGCCCCGCAG GGACCCCAGG ACAAATAGAT 
TGTGACACAG ATGTGAAAAG GGCCGTTGGA GGTGACAGAC AGGAGGCCAT CCAGCCAGGT 
TGCATAGGAG GGCCCAAGGG ATTGCCAGGC CTGCCAGGAC CCCCAGGCCC CACAGGTGCC 
AAAGGCCTCC GAGGAATCCC AGGCTTCGCA GGAGCTGATG GAGGACCAGG GCCCAGGGGC 
TTGCCAGGAG ACGCAGGTCG TGAAGGGTTC CCAGGACCCC CAGGGTTCAT AGGACCCCGA 
GGATCCAAAG GTGCAGTGGG CCTCCCTGGC CCAGATGGAT CCCCAGGTCC CATCGGCCTG 
CCAGGGCCAG ATGGGCCCCC TGGGGAAAGG GGCCTCCCTG GAGAAGTCCT GGGAGCTCAG 
CCCGGGCCAC GGGGAGATGC TGGTGTGCCT GGACAGCCTG GGCTTAAAGG CCTTCCCGGA 
GACAGAGGCC CCCCTGGATT CAGAGGAAGC CAAGGGATGC CTGGGATGCC AGGGCTGAAG 
GGCCAGCCAG GCCTCCCAGG ACCTTCCGGC CAGCCAGGCC TGTATGGGCC TCCAGGACTG 
CATGGATTCC CAGGAGCTCC TGGCCAAGAG GGGCCCTTGG GGCTGCCAGG AATCCCAGGC 
CGTGAAGGTC TGCCTGGTGA TAGAGGGGAC CCTGGGGACA CAGGCGCTCC TGGCCCTGTG 
GGCATGAAAG GTCTCTCTGG TGACAGAGGA GATGCTGGCT TCACAGGGGA GCAAGGCCAT 
CCAGGAAGCC CTGGATTTAA AGGAATTGAT GGAATGCCTG GGACCCCCGG GCTAAAAGGA 
GATAGAGGCT CACCTGGGAT GGATGGTTTC CAAGGCATGC CTGGACTCAA AGGGAGACCC 
GGGTTTCCAG GGAGCAAAGG CGAGGCTGGA TTTTTCGGAA TACCCGGTCT GAAGGGTCTG 
GCTGGTGAGC CAGGTTTTAA AGGCAGCCGA GGGGACCCTG GGCCCCCAGG ACCACCTCCT 
GTCATCCTGC CAGGAATGAA AGACATTAAA GGAGAGAAAG GAGATGAAGG GCCTATGGGG 
CTGAAAGGAT ACCTGGGCGC AAAAGGTATC CAAGGAATGC CAGGCATCCC AGGGCTGTCA 
GGAATCCCTG GGCTGCCTGG GAGGCCCGGC CACATCAAAG GAGTCAAGGG AGACATCGGA 
GTCCCCGGCA TCCCCGGTTT GCCAGGATTC CCTGGGGTGG CTGGCCCCCC TGGAATTACG 
GGATTCCCAG GATTCATAGG AAGCCGGGGT GACAAAGGTG CCCCAGGGAG AGCAGGCCTG 
TATGGCGAGA TTGGCGCGAC TGGTGATTTC GGTGACATCG GGGACACTAT AAATTTACCA 
GGAAGACCAG GCCTGAAGGG GGAGCGGGGC ACCACTGGAA TACCAGGTCT GAAGGGATTC 
TTTGGAGAGA AGGGAACAGA AGGTGACATC GGCTTCCCTG GGATAACAGG CGTGACTGGA 
GTCCAAGGCC CTCCTGGACT TAAAGGACAA ACAGGCTTTC CAGGGCTGAC TGGGCCTCCA 
GGGTCGCAGG GAGAGCTGGG GCGGATTGGA CTGCCTGGTG GCAAAGGAGA TGATGGCTGG 
CCGGGAGCTC CGGGCTTACC AGGTTTTCCG GGACTCCGTG GGATCCGCGG CTTACACGGC 
TTGCCAGGCA CCAAGGGCTT TCCAGGATCC CCAGGTTCTG ACATCCACGG AGACCCAGGC 
TTCCCAGGCC CTCCTGGGGA AAGAGGTGAC CCAGGAGAGG CCAACACCCT TCCAGGCCCT 
GTGGGAGTCC CAGGACAGAA AGGAGACCAA GGAGCTCCAG GGGAACGAGG CCCACCTGGG 
AGCCCAGGAC TTCAGGGGTT CCCAGGCATC ACACCCCCTT CCAACATCTC TGGGGCACCT 
GGTGACAAAG GGGCGCCAGG GATATTTGGC CTGAAAGGTT ATCGGGGCCC ACCAGGGCCA 
CCAGGTTCTG CTGCTCTTCC TGGAAGCAAA GGTGACACAG GGAACCCAGG AGCTCCAGGA 
ACCCCAGGGA CCAAAGGATG GGCCGGGGAC TCCGGGCCCC AGGGCAGGCC TGGTGTGTTT 
GGTCTCCCAG GAGAAAAAGG GCCCAGGGGT GAACAAGGCT TCATGGGGAA CACTGGACCC 
ACCGGGGCGG TGGGCGACAG AGGCCCCAAG GGACCCAAGG GAGACCCAGG ATTCCCTGGT 
GCCCCCGGGA CTGTGGGAGC CCCCGGGATT GCAGGAATCC CCCAGAAGAT TGCCGTCCAA 
CCAGGGACAG TGGGTCCCCA GGGGAGGCGA GGCCCCCCTG GGGCACCGGG GGAGATGGGG 
CCCCAGGGCC CCCCCGGAGA ACCAGGTTTT CGTGGGGCTC CAGGGAAAGC TGGGCCCCAA 
GGAAGAGGTG GTGTGTCTGC TGTTCCCGGC TTCCGGGGAG ATGAAGGACC CATAGGCCAC 
CAGGGGCCGA TTGGCCAAGA AGGTGCACCA GGCCGTCCAG GGAGCCCGGG CCTGCCGGGT 
ATGCCAGGCC GCAGCGTCAG CATCGGCTAC CTCCTGGTGA AGCACAGCCA GACGGACCAG 
GAGCCCATGT GCCCAGTGGG CATGAACAAA CTCTGGAGTG GATACAGCCT GCTGTACTTC 
GAGGGCCAGG AGAAGGCGCA CAACCAGGAC CTGGGGCTGG CGGGCTCCTG CCTGGCGCGG 
TTCAGCACCA TGCCCTTCCT GTACTGCAAC CCTGGTGATG TCTGCTACTA TGCCAGCCGG 
AACGACAAGT CCTACTGGCT CTCTACCACT GCGCCGCTGC CCATGATGCC CGTGGCCGAG 
GACGAGATCA AGCCCTACAT CAGCCGCTGT TCTGTGTGTG AGGCCCCGGC CATCGCCATC 
GCGGTCCACA GTCAGGATGT CTCCATCCCA CACTGCCCAG CTGGGTGGCG GAGTTTGTGG 
ATCGGATATT CCTTCCTCAT GCACACGGCG GCGGGAGACG AAGGCGGTGG CCAATCACTG 
GTGTCACCGG GCAGCTGTCT AGAGGACTTC CGCGCCACAC CATTCATCGA ATGCAATGGA 
GGCCGCGGCA CCTGCCACTA CTACGCCAAC AAGTACAGCT TCTGGCTGAC CACCATTCCC 
GAGCAGAGCT TCCAGGGCTC GCCCTCCGCC GACACGCTCA AGGCCGGCCT CATCCGCACA 
CACATCAGCC GCTGCCAGGT GTGCATGAAG AACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001837.2
CDS	307..5445
Translation	MGRDQRAVAGPALRRWLLLGTVTVGFLAQSVLAGVKKFDVPCGGRDCSGGCQCYPEKGGRGQPGPVGPQGYNGPPGLQGFPGLQGRKGDKGERGAPGVTGPKGDVGARGVSGFPGADGIPGHPGQGGPRGRPGYDGCNGTQGDSGPQGPPGSEGFTGPPGPQGPKGQKGEPYALPKEERDRYRGEPGEPGLVGFQGPPGRPGHVGQMGPVGAPGRPGPPGPPGPKGQQGNRGLGFYGVKGEKGDVGQPGPNGIPSDTLHPIIAPTGVTFHPDQYKGEKGSEGEPGIRGISLKGEEGIMGFPGLRGYPGLSGEKGSPGQKGSRGLDGYQGPDGPRGPKGEAGDPGPPGLPAYSPHPSLAKGARGDPGFPGAQGEPGSQGEPGDPGLPGPPGLSIGDGDQRRGLPGEMGPKGFIGDPGIPALYGGPPGPDGKRGPPGPPGLPGPPGPDGFLFGLKGAKGRAGFPGLPGSPGARGPKGWKGDAGECRCTEGDEAIKGLPGLPGPKGFAGINGEPGRKGDRGDPGQHGLPGFPGLKGVPGNIGAPGPKGAKGDSRTITTKGERGQPGVPGVPGMKGDDGSPGRDGLDGFPGLPGPPGDGIKGPPGDPGYPGIPGTKGTPGEMGPPGLGLPGLKGQRGFPGDAGLPGPPGFLGPPGPAGTPGQIDCDTDVKRAVGGDRQEAIQPGCIGGPKGLPGLPGPPGPTGAKGLRGIPGFAGADGGPGPRGLPGDAGREGFPGPPGFIGPRGSKGAVGLPGPDGSPGPIGLPGPDGPPGERGLPGEVLGAQPGPRGDAGVPGQPGLKGLPGDRGPPGFRGSQGMPGMPGLKGQPGLPGPSGQPGLYGPPGLHGFPGAPGQEGPLGLPGIPGREGLPGDRGDPGDTGAPGPVGMKGLSGDRGDAGFTGEQGHPGSPGFKGIDGMPGTPGLKGDRGSPGMDGFQGMPGLKGRPGFPGSKGEAGFFGIPGLKGLAGEPGFKGSRGDPGPPGPPPVILPGMKDIKGEKGDEGPMGLKGYLGAKGIQGMPGIPGLSGIPGLPGRPGHIKGVKGDIGVPGIPGLPGFPGVAGPPGITGFPGFIGSRGDKGAPGRAGLYGEIGATGDFGDIGDTINLPGRPGLKGERGTTGIPGLKGFFGEKGTEGDIGFPGITGVTGVQGPPGLKGQTGFPGLTGPPGSQGELGRIGLPGGKGDDGWPGAPGLPGFPGLRGIRGLHGLPGTKGFPGSPGSDIHGDPGFPGPPGERGDPGEANTLPGPVGVPGQKGDQGAPGERGPPGSPGLQGFPGITPPSNISGAPGDKGAPGIFGLKGYRGPPGPPGSAALPGSKGDTGNPGAPGTPGTKGWAGDSGPQGRPGVFGLPGEKGPRGEQGFMGNTGPTGAVGDRGPKGPKGDPGFPGAPGTVGAPGIAGIPQKIAVQPGTVGPQGRRGPPGAPGEMGPQGPPGEPGFRGAPGKAGPQGRGGVSAVPGFRGDEGPIGHQGPIGQEGAPGRPGSPGLPGMPGRSVSIGYLLVKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEDEIKPYISRCSVCEAPAIAIAVHSQDVSIPHCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYYANKYSFWLTTIPEQSFQGSPSADTLKAGLIRTHISRCQVCMKNL

Target ORF information:

RefSeq Version	NM_001846.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 2 chain (COL4A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001846.3

ORF Insert Sequence:

ATGGGGAGAG ACCAGCGCGC GGTGGCCGGC CCTGCCCTAC GGCGGTGGCT GCTGCTGGGG 
ACAGTGACCG TGGGGTTCCT CGCCCAGAGC GTCTTGGCGG GTGTGAAGAA GTTTGATGTG 
CCGTGTGGAG GAAGAGATTG CAGTGGGGGC TGCCAGTGCT ACCCTGAGAA AGGTGGACGT 
GGTCAGCCTG GGCCAGTGGG CCCCCAGGGG TACAATGGGC CACCAGGATT ACAAGGATTC 
CCGGGACTGC AGGGACGTAA AGGAGACAAG GGTGAAAGGG GAGCCCCCGG AGTAACGGGA 
CCCAAGGGCG ACGTGGGAGC AAGAGGCGTT TCTGGATTCC CTGGTGCCGA TGGAATTCCT 
GGACACCCGG GGCAAGGTGG GCCCAGGGGA AGGCCGGGCT ACGATGGCTG CAACGGAACC 
CAGGGAGACT CAGGTCCACA GGGGCCCCCC GGCTCTGAGG GGTTCACCGG GCCTCCCGGG 
CCCCAAGGAC CAAAAGGGCA GAAAGGTGAG CCTTATGCAC TGCCTAAAGA GGAGCGCGAC 
AGATATCGGG GTGAACCTGG AGAGCCTGGA TTGGTCGGTT TCCAGGGACC TCCCGGCCGC 
CCTGGGCATG TGGGACAGAT GGGTCCAGTT GGAGCTCCAG GGAGACCAGG ACCACCTGGA 
CCCCCTGGAC CAAAAGGACA GCAAGGCAAC AGAGGACTTG GTTTCTACGG AGTTAAGGGT 
GAAAAGGGTG ACGTAGGGCA GCCGGGACCC AACGGGATTC CATCAGACAC CCTCCACCCC 
ATCATCGCGC CCACAGGAGT CACCTTCCAC CCAGATCAGT ACAAGGGTGA AAAAGGCAGT 
GAGGGGGAAC CAGGAATAAG AGGCATTTCC TTGAAGGGAG AAGAAGGAAT CATGGGCTTT 
CCTGGACTGA GGGGTTACCC TGGCTTGAGT GGTGAAAAAG GATCACCAGG ACAGAAGGGA 
AGCCGAGGCC TGGATGGCTA TCAAGGGCCT GATGGACCCC GGGGACCCAA GGGAGAAGCC 
GGAGACCCAG GGCCCCCTGG ACTACCTGCC TACTCCCCTC ACCCTTCCCT AGCAAAAGGT 
GCCAGAGGTG ACCCGGGATT CCCAGGGGCC CAAGGGGAGC CAGGAAGCCA GGGTGAGCCA 
GGAGACCCGG GCCTCCCAGG TCCCCCTGGC CTCTCCATCG GAGATGGAGA TCAGAGGAGA 
GGCCTGCCGG GTGAGATGGG ACCCAAGGGC TTCATCGGAG ACCCCGGCAT CCCTGCGCTC 
TACGGGGGCC CACCTGGACC TGATGGAAAG CGAGGGCCTC CAGGACCCCC CGGGCTCCCT 
GGACCACCTG GACCTGATGG CTTCCTGTTT GGGCTGAAAG GAGCAAAAGG AAGAGCAGGC 
TTCCCTGGGC TTCCCGGCTC CCCTGGAGCC CGCGGACCAA AGGGGTGGAA AGGTGACGCT 
GGGGAATGCA GATGTACAGA AGGCGACGAA GCTATCAAAG GTCTTCCGGG ACTGCCAGGA 
CCCAAGGGCT TCGCAGGCAT CAACGGGGAG CCGGGGAGGA AAGGGGACAG AGGAGACCCC 
GGCCAACACG GCCTCCCTGG GTTCCCAGGG CTCAAGGGAG TGCCTGGCAA CATTGGTGCT 
CCCGGACCCA AAGGAGCAAA AGGAGATTCC AGAACAATCA CAACCAAAGG TGAGCGGGGA 
CAGCCCGGCG TCCCAGGTGT GCCCGGGATG AAAGGTGACG ATGGCAGCCC AGGCCGCGAT 
GGGCTCGATG GATTCCCCGG CCTCCCAGGC CCTCCCGGTG ATGGCATCAA GGGCCCTCCA 
GGGGACCCAG GCTATCCAGG AATACCTGGA ACGAAGGGTA CTCCAGGAGA AATGGGCCCC 
CCAGGACTGG GCCTTCCCGG CCTCAAAGGC CAACGTGGTT TCCCTGGAGA CGCCGGCTTA 
CCTGGACCAC CAGGCTTCCT GGGCCCTCCT GGCCCCGCAG GGACCCCAGG ACAAATAGAT 
TGTGACACAG ATGTGAAAAG GGCCGTTGGA GGTGACAGAC AGGAGGCCAT CCAGCCAGGT 
TGCATAGGAG GGCCCAAGGG ATTGCCAGGC CTGCCAGGAC CCCCAGGCCC CACAGGTGCC 
AAAGGCCTCC GAGGAATCCC AGGCTTCGCA GGAGCTGATG GAGGACCAGG GCCCAGGGGC 
TTGCCAGGAG ACGCAGGTCG TGAAGGGTTC CCAGGACCCC CAGGGTTCAT AGGACCCCGA 
GGATCCAAAG GTGCAGTGGG CCTCCCTGGC CCAGATGGAT CCCCAGGTCC CATCGGCCTG 
CCAGGGCCAG ATGGGCCCCC TGGGGAAAGG GGCCTCCCTG GAGAAGTCCT GGGAGCTCAG 
CCCGGGCCAC GGGGAGATGC TGGTGTGCCT GGACAGCCTG GGCTTAAAGG CCTTCCCGGA 
GACAGAGGCC CCCCTGGATT CAGAGGAAGC CAAGGGATGC CTGGGATGCC AGGGCTGAAG 
GGCCAGCCAG GCCTCCCAGG ACCTTCCGGC CAGCCAGGCC TGTATGGGCC TCCAGGACTG 
CATGGATTCC CAGGAGCTCC TGGCCAAGAG GGGCCCTTGG GGCTGCCAGG AATCCCAGGC 
CGTGAAGGTC TGCCTGGTGA TAGAGGGGAC CCTGGGGACA CAGGCGCTCC TGGCCCTGTG 
GGCATGAAAG GTCTCTCTGG TGACAGAGGA GATGCTGGCT TCACAGGGGA GCAAGGCCAT 
CCAGGAAGCC CTGGATTTAA AGGAATTGAT GGAATGCCTG GGACCCCCGG GCTAAAAGGA 
GATAGAGGCT CACCTGGGAT GGATGGTTTC CAAGGCATGC CTGGACTCAA AGGGAGACCC 
GGGTTTCCAG GGAGCAAAGG CGAGGCTGGA TTTTTCGGAA TACCCGGTCT GAAGGGTCTG 
GCTGGTGAGC CAGGTTTTAA AGGCAGCCGA GGGGACCCTG GGCCCCCAGG ACCACCTCCT 
GTCATCCTGC CAGGAATGAA AGACATTAAA GGAGAGAAAG GAGATGAAGG GCCTATGGGG 
CTGAAAGGAT ACCTGGGCGC AAAAGGTATC CAAGGAATGC CAGGCATCCC AGGGCTGTCA 
GGAATCCCTG GGCTGCCTGG GAGGCCCGGC CACATCAAAG GAGTCAAGGG AGACATCGGA 
GTCCCCGGCA TCCCCGGTTT GCCAGGATTC CCTGGGGTGG CTGGCCCCCC TGGAATTACG 
GGATTCCCAG GATTCATAGG AAGCCGGGGT GACAAAGGTG CCCCAGGGAG AGCAGGCCTG 
TATGGCGAGA TTGGCGCGAC TGGTGATTTC GGTGACATCG GGGACACTAT AAATTTACCA 
GGAAGACCAG GCCTGAAGGG GGAGCGGGGC ACCACTGGAA TACCAGGTCT GAAGGGATTC 
TTTGGAGAGA AGGGAACAGA AGGTGACATC GGCTTCCCTG GGATAACAGG CGTGACTGGA 
GTCCAAGGCC CTCCTGGACT TAAAGGACAA ACAGGCTTTC CAGGGCTGAC TGGGCCTCCA 
GGGTCGCAGG GAGAGCTGGG GCGGATTGGA CTGCCTGGTG GCAAAGGAGA TGATGGCTGG 
CCGGGAGCTC CGGGCTTACC AGGTTTTCCG GGACTCCGTG GGATCCGCGG CTTACACGGC 
TTGCCAGGCA CCAAGGGCTT TCCAGGATCC CCAGGTTCTG ACATCCACGG AGACCCAGGC 
TTCCCAGGCC CTCCTGGGGA AAGAGGTGAC CCAGGAGAGG CCAACACCCT TCCAGGCCCT 
GTGGGAGTCC CAGGACAGAA AGGAGACCAA GGAGCTCCAG GGGAACGAGG CCCACCTGGG 
AGCCCAGGAC TTCAGGGGTT CCCAGGCATC ACACCCCCTT CCAACATCTC TGGGGCACCT 
GGTGACAAAG GGGCGCCAGG GATATTTGGC CTGAAAGGTT ATCGGGGCCC ACCAGGGCCA 
CCAGGTTCTG CTGCTCTTCC TGGAAGCAAA GGTGACACAG GGAACCCAGG AGCTCCAGGA 
ACCCCAGGGA CCAAAGGATG GGCCGGGGAC TCCGGGCCCC AGGGCAGGCC TGGTGTGTTT 
GGTCTCCCAG GAGAAAAAGG GCCCAGGGGT GAACAAGGCT TCATGGGGAA CACTGGACCC 
ACCGGGGCGG TGGGCGACAG AGGCCCCAAG GGACCCAAGG GAGACCCAGG ATTCCCTGGT 
GCCCCCGGGA CTGTGGGAGC CCCCGGGATT GCAGGAATCC CCCAGAAGAT TGCCGTCCAA 
CCAGGGACAG TGGGTCCCCA GGGGAGGCGA GGCCCCCCTG GGGCACCGGG GGAGATGGGG 
CCCCAGGGCC CCCCCGGAGA ACCAGGTTTT CGTGGGGCTC CAGGGAAAGC TGGGCCCCAA 
GGAAGAGGTG GTGTGTCTGC TGTTCCCGGC TTCCGGGGAG ATGAAGGACC CATAGGCCAC 
CAGGGGCCGA TTGGCCAAGA AGGTGCACCA GGCCGTCCAG GGAGCCCGGG CCTGCCGGGT 
ATGCCAGGCC GCAGCGTCAG CATCGGCTAC CTCCTGGTGA AGCACAGCCA GACGGACCAG 
GAGCCCATGT GCCCAGTGGG CATGAACAAA CTCTGGAGTG GATACAGCCT GCTGTACTTC 
GAGGGCCAGG AGAAGGCGCA CAACCAGGAC CTGGGGCTGG CGGGCTCCTG CCTGGCGCGG 
TTCAGCACCA TGCCCTTCCT GTACTGCAAC CCTGGTGATG TCTGCTACTA TGCCAGCCGG 
AACGACAAGT CCTACTGGCT CTCTACCACT GCGCCGCTGC CCATGATGCC CGTGGCCGAG 
GACGAGATCA AGCCCTACAT CAGCCGCTGT TCTGTGTGTG AGGCCCCGGC CATCGCCATC 
GCGGTCCACA GTCAGGATGT CTCCATCCCA CACTGCCCAG CTGGGTGGCG GAGTTTGTGG 
ATCGGATATT CCTTCCTCAT GCACACGGCG GCGGGAGACG AAGGCGGTGG CCAATCACTG 
GTGTCACCGG GCAGCTGTCT AGAGGACTTC CGCGCCACAC CATTCATCGA ATGCAATGGA 
GGCCGCGGCA CCTGCCACTA CTACGCCAAC AAGTACAGCT TCTGGCTGAC CACCATTCCC 
GAGCAGAGCT TCCAGGGCTC GCCCTCCGCC GACACGCTCA AGGCCGGCCT CATCCGCACA 
CACATCAGCC GCTGCCAGGT GTGCATGAAG AACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neurology89(17)1829-1839(2017 Oct)
Rannikm?e K,Sivakumaran V,Millar H,Malik R,Anderson CD,Chong M,Dave T,Falcone GJ,Fernandez-Cadenas I,Jimenez-Conde J,Lindgren A,Montaner J,O'Donnell M,Par? G,Radmanesh F,Rost NS,Slowik A,S?derholm M,Traylor M,Pulit SL,Seshadri S,Worrall BB,Woo D,Markus HS,Mitchell BD,Dichgans M,Rosand J,Sudlow CLM,

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COL4A2 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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