Col4a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col4a2
Entrez Gene ID	12827
Full Name	collagen, type IV, alpha 2
Synonyms	Col4a-2
General protein information	Preferred Names collagen, type IV, alpha 2 Names collagen alpha-2(IV) chain canstatin procollagen, type IV, alpha 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 A1.1\|8 5.62 cM
Summary	This gene encodes the alpha-2 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of alpha-1 and alpha-2 subunits that assembles into a type IV collagen network. Canstatin, a peptide derived fom the C-terminus of the collagen chain, is a matrikine that has been shown to inhibit angiogenesis. Homozygous knockout mice for this gene exhibit impaired basement membrane integrity and embryonic lethality. This gene shares a bi-directional promoter with a related gene on chromosome 8. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009932.4	NP_034062.3	collagen alpha-2(IV) chain precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL4A2	XP_001136859.1
Canis lupus familiaris (dog)	COL4A2	XP_534183.3
Rattus norvegicus (Norway rat)	Col4a2	XP_006253538.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009200	XP_319976.3
	COL4A2	XP_002800878.1
Drosophila melanogaster (fruit fly)	Cg25C	NP_723046.1
Caenorhabditis elegans (roundworm)	emb-9	NP_001022662.1
Bos taurus (cattle)	COL4A2	XP_002692057.2
Mus musculus (house mouse)	Col4a2	NP_034062.3
Gallus gallus (chicken)	COL4A2	NP_001155862.1
Danio rerio (zebrafish)	col4a2	XP_687811.6
Caenorhabditis elegans (roundworm)	let-2	NP_510663.1
Xenopus tropicalis (tropical clawed frog)	col4a2	XP_004912045.1
Homo sapiens (human)	COL4A2	NP_001837.2

Gene Ontology
Bibliography

Related articles in PubMed

Lhx8 ablation leads to massive autophagy of mouse oocytes associated with DNA damage.
Pelosi E, D'Ignazio L, Michel M, Beyer M, Thompson K, Forabosco A, Schlessinger D
Biology of reproduction(2018 Jan)

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in murine Col4a1 and Col4a2 genes affected the balance between lung epithelial progenitors and differentiated cells. Mutations in Col4a1 derived from the vascular component were sufficient to cause defects in vascular development and the blood-gas barrier. Col4a1 and Col4a2 mutants displayed disrupted myofibroblast proliferation, differentiation and migration.
Title: Type IV collagen drives alveolar epithelial-endothelial association and the morphogenetic movements of septation.

COL4A1 and COL4A2 mutations are pleiotropic and cause a wide spectrum of disorders, including ocular dysgenesis, brain malformations and myopathy, of variable severity in both mice and humans.
Title: Allelic heterogeneity contributes to variability in ocular dysgenesis, myopathy and brain malformations caused by Col4a1 and Col4a2 mutations.

WT1 maintains testicular cord integrity by additively regulating the expression of basal lamina components Col4a1 and Col4a2 with SOX9.
Title: The Wilms tumor gene, Wt1, maintains testicular cord integrity by regulating the expression of Col4a1 and Col4a2.

Data suggest that podocyte von Hippel-Lindau protein is required for normal maintenance of podocytes, basment membrane collagen {alpha}1{alpha}2{alpha}1(IV) deposition and ultrastructure, neuroglobin experssion and glomerular barrier properties.
Title: Deletion of von Hippel-Lindau in glomerular podocytes results in glomerular basement membrane thickening, ectopic subepithelial deposition of collagen {alpha}1{alpha}2{alpha}1(IV), expression of neuroglobin, and proteinuria.

These results indicate that SOX9 is essential for Col4a2 expression in mesangial cells and might be involved in the accumulation of alpha2(IV) collagen in experimental nephritis.
Title: SRY-related HMG box 9 regulates the expression of Col4a2 through transactivating its enhancer element in mesangial cells.

The following Col4a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col4a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19209	NM_009932.4 Latest version!	Mus musculus collagen, type IV, alpha 2 (Col4a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1252.30 $1789.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col4a2 ( NM_009932.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034062.3 Mus musculus collagen, type IV, alpha 2 (Col4a2), mRNA.

Price & Availability↑

CloneID	OMu19209
Clone ID Related Accession (Same CDS sequence)	NM_009932.4
Accession Version	NM_009932.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	collagen alpha-2(IV) chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ125538.1, AK053858.1, AC114598.9, BC138040.1, AK075619.1 and CO044160.1. On Aug 8, 2015 this sequence version replaced NM_009932.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J04695.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGAG TGCGGTTCAA AGCGTCTGGC CCTCCCCTGC GAGGGTGGCT GCTGCTAGCA 
ACTGTGACAG TGGGACTCCT GGCTCAGAGC GTCTTGGGGG GTGTGAAGAA GTTGGATGTG 
CCCTGCGGAG GGAGAGACTG CAGTGGGGGT TGCCAGTGCT ACCCGGAGAA AGGAGCAAGG 
GGTCAGCCAG GGGCAGTGGG CCCCCAGGGG TACAATGGCC CCCCAGGGTT GCAAGGATTC 
CCAGGACTAC AGGGCCGCAA AGGAGACAAG GGCGAACGGG GAGTTCCTGG ACCAACTGGA 
CCAAAAGGAG ATGTGGGAGC GAGAGGCGTC TCTGGATTCC CCGGTGCAGA TGGAATTCCT 
GGGCATCCAG GGCAAGGCGG ACCCCGAGGA AGGCCAGGCT ACGATGGTTG CAACGGAACC 
AGGGGAGACG CGGGTCCACA GGGGCCCTCT GGATCTGGGG GCTTCCCTGG CCTCCCTGGG 
CCCCAAGGAC CCAAGGGGCA GAAAGGCGAG CCTTACGCAC TCTCTAAAGA AGACCGTGAC 
AAATACCGGG GTGAACCTGG AGAGCCTGGC CTGGTTGGCT ACCAGGGACC TCCTGGCCGC 
CCAGGGCCCA TAGGACAGAT GGGTCCCATG GGAGCACCTG GAAGACCGGG ACCACCGGGA 
CCCCCTGGAC CCAAGGGACA ACCAGGCAAC CGAGGGCTCG GTTTTTACGG ACAGAAGGGT 
GAAAAGGGTG ACATAGGACA GCCAGGACCC AACGGGATCC CATCTGACAT CACACTTGTT 
GGGCCCACAA CATCAACGAT CCACCCGGAT CTGTACAAGG GTGAAAAAGG AGACGAAGGA 
GAGCAAGGGA TACCCGGCGT AATCTCAAAA GGCGAGGAAG GCATCATGGG GTTCCCAGGA 
ATACGGGGTT TTCCTGGCCT TGATGGAGAA AAAGGAGTCG TAGGACAGAA AGGAAGCAGA 
GGCCTGGATG GTTTCCAAGG CCCCAGTGGA CCTCGAGGAC CCAAGGGAGA ACGGGGGGAA 
CAAGGACCCC CAGGACCCTC TGTCTACTCG CCCCATCCAT CCCTGGCAAA AGGTGCCCGA 
GGTGACCCAG GATTCCAAGG TGCTCATGGG GAGCCAGGAA GCCGAGGCGA ACCGGGAGAG 
CCTGGAACCG CGGGTCCTCC AGGCCCATCC GTCGGAGATG AAGATTCAAT GAGAGGCCTT 
CCAGGGGAGA TGGGACCCAA AGGCTTCTCA GGAGAACCAG GGTCCCCTGC ACGCTATCTT 
GGCCCACCAG GAGCAGATGG AAGACCAGGT CCCCAAGGAG TCCCTGGACC TGCAGGCCCA 
CCTGGACCAG ATGGCTTCCT GTTTGGCCTG AAAGGATCAG AAGGGAGAGT GGGCTACCCG 
GGACCTTCAG GTTTCCCAGG AACACGAGGG CAGAAAGGAT GGAAAGGTGA AGCTGGAGAC 
TGCCAATGTG GCCAGGTCAT CGGGGGTCTT CCGGGACTGC CAGGACCCAA AGGTTTTCCC 
GGTGTCAATG GGGAGCTCGG AAAGAAAGGC GACCAAGGAG ACCCAGGCCT GCACGGTATC 
CCTGGGTTCC CAGGATTCAA GGGAGCCCCG GGCGTTGCTG GAGCTCCTGG ACCCAAAGGA 
ATAAAGGGAG ACTCTAGAAC AATCACCACC AAAGGGGAGC GAGGACAGCC AGGTATCCCT 
GGTGTACACG GAATGAAAGG TGATGACGGC GTCCCAGGAC GGGATGGGCT GGATGGATTC 
CCTGGCCTGC CTGGCCCCCC GGGCGATGGC ATCAAAGGCC CTCCAGGGGA TGCAGGTCTC 
CCTGGTGTAC CCGGAACCAA GGGCTTTCCA GGAGACATCG GTCCTCCAGG ACAGGGCTTA 
CCAGGTCCGA AAGGCGAGCG TGGTTTCCCT GGAGACGCTG GGTTGCCTGG ACCTCCGGGC 
TTCCCTGGTC CTCCAGGTCC CCCAGGAACC CCAGGACAGA GAGATTGTGA CACGGGTGTG 
AAAAGACCTA TCGGAGGCGG CCAGCAAGTG GTCGTTCAGC CAGGTTGCAT TGAAGGCCCC 
ACAGGATCAC CTGGCCAGCC AGGACCCCCA GGCCCCACAG GTGCCAAAGG CGTCAGAGGA 
ATGCCAGGAT TCCCAGGAGC TTCTGGAGAA CAAGGGCTCA AGGGCTTTCC AGGAGACCCA 
GGTCGAGAAG GCTTCCCAGG GCCCCCAGGG TTCATGGGAC CCCGAGGATC CAAAGGTACC 
ACAGGCCTCC CTGGCCCAGA TGGACCCCCA GGCCCCATTG GACTTCCAGG ACCAGCTGGA 
CCCCCTGGGG ACAGAGGGAT TCCAGGAGAA GTGCTAGGGG CCCAGCCTGG GACACGAGGA 
GATGCTGGAC TTCCAGGACA GCCTGGGCTG AAAGGGCTCC CAGGAGAAAC AGGAGCACCG 
GGATTCAGAG GAAGCCAAGG GATGCCCGGG ATGCCAGGGC TTAAAGGTCA GCCAGGCTTC 
CCAGGACCTT CGGGACAGCC AGGACAGTCT GGGCCCCCAG GACAACATGG ATTCCCAGGA 
ACTCCTGGCC GAGAGGGGCC TTTGGGGCAG CCAGGCTCCC CTGGCCTTGG AGGTTTGCCT 
GGAGACAGGG GCGAGCCTGG TGACCCTGGT GTCCCTGGCC CTGTGGGCAT GAAAGGTCTG 
TCTGGGGACA GAGGTGACGC CGGTATGTCA GGCGAGCGTG GTCACCCTGG AAGCCCTGGA 
TTTAAAGGAA TGGCAGGGAT GCCTGGTATC CCTGGGCAGA AAGGTGATCG AGGGTCGCCT 
GGAATGGACG GTTTCCAGGG CATGTTGGGA CTTAAGGGAA GACAGGGCTT CCCAGGGACC 
AAAGGAGAAG CTGGATTTTT TGGAGTTCCT GGTTTGAAGG GCCTGCCTGG CGAGCCAGGG 
GTTAAAGGAA ACCGAGGAGA CCGGGGGCCC CCAGGACCAC CTCCTCTCAT CCTGCCGGGG 
ATGAAGGACA TCAAGGGAGA AAAGGGAGAT GAAGGACCAA TGGGCCTGAA AGGGTATCTG 
GGCTTAAAAG GCATCCAAGG AATGCCCGGA GTCCCCGGAG TGTCTGGATT CCCTGGGCTA 
CCTGGAAGGC CTGGCTTCAT CAAAGGAGTC AAGGGAGACA TCGGAGTCCC TGGCACACCA 
GGCTTGCCGG GATTCCCTGG GGTGTCTGGC CCTCCTGGAA TTACCGGGTT TCCAGGATTC 
ACAGGCAGCC GGGGCGAGAA GGGTACTCCA GGAGTAGCAG GAGTTTTTGG CGAGACCGGC 
CCTACTGGGG ACTTTGGTGA CATTGGGGAC ACTGTGGACT TACCAGGGAG CCCAGGCCTG 
AAGGGGGAAC GGGGCATCAC GGGAATTCCA GGTCTCAAGG GATTCTTTGG AGAGAAAGGA 
GCTGCGGGTG ACATTGGATT TCCTGGGATA ACGGGCATGG CTGGAGCTCA GGGCTCTCCT 
GGACTTAAAG GACAGACAGG CTTTCCAGGG CTCACAGGGC TGCAAGGGCC ACAGGGAGAG 
CCTGGACGGA TCGGAATACC CGGTGACAAG GGTGATTTCG GCTGGCCAGG CGTACCAGGT 
CTACCAGGTT TTCCTGGGAT CCGGGGCATC AGCGGATTGC ACGGCCTGCC AGGCACCAAA 
GGCTTCCCTG GATCACCAGG TGTTGATGCT CATGGAGACC CAGGGTTCCC AGGCCCCACT 
GGAGACAGGG GTGACCGGGG AGAGGCCAAC ACACTTCCAG GCCCCGTGGG AGTTCCAGGG 
CAGAAAGGGG AGCGGGGAAC CCCAGGGGAA CGTGGCCCAG CTGGAAGCCC AGGACTTCAG 
GGTTTCCCTG GTATCTCTCC ACCATCCAAC ATCTCTGGGT CACCTGGTGA TGTAGGCGCA 
CCAGGAATAT TTGGCTTGCA AGGCTACCAA GGCCCTCCAG GACCACCTGG GCCAAACGCA 
CTTCCTGGAA TCAAAGGAGA TGAGGGGAGC TCTGGAGCTG CAGGATTCCC TGGACAGAAA 
GGATGGGTTG GGGACCCAGG GCCCCAGGGC CAGCCTGGTG TACTCGGTCT TCCGGGGGAG 
AAAGGGCCCA AGGGTGAACA AGGATTCATG GGCAACACCG GGCCCTCTGG GGCCGTGGGT 
GACAGAGGCC CCAAAGGACC CAAAGGCGAC CAAGGATTCC CAGGTGCTCC TGGCTCTATG 
GGGTCCCCAG GAATTCCTGG CATCCCCCAG AAGATTGCTG TCCAGCCTGG AACGCTGGGT 
CCCCAGGGCA GGAGAGGCCT TCCTGGGGCC CTGGGAGAGA TAGGGCCGCA GGGCCCTCCT 
GGAGATCCAG GCTTCCGTGG GGCCCCAGGC AAGGCTGGGC CTCAGGGCAG AGGTGGCGTG 
TCTGCTGTTC CAGGGTTCCG GGGAGACCAA GGGCCCATGG GACACCAGGG TCCAGTTGGC 
CAGGAAGGGG AGCCAGGCCG TCCAGGGAGC CCAGGCCTGC CAGGGATGCC AGGCCGCAGT 
GTGAGCATCG GCTACCTCCT GGTGAAGCAC AGCCAAACGG ACCAGGAACC CATGTGCCCT 
GTGGGCATGA ACAAGCTCTG GAGTGGGTAC AGCCTGCTAT ATTTTGAGGG CCAGGAGAAA 
GCGCACAACC AGGACCTAGG ACTGGCAGGC TCCTGCCTGG CACGCTTCAG CACCATGCCT 
TTCCTGTACT GCAATCCGGG TGACGTCTGC TACTATGCCA GCCGCAACGA CAAGTCCTAC 
TGGCTCTCCA CCACGGCCCC TCTGCCCATG ATGCCCGTGG CTGAGGAGGA AATCAAGCCC 
TACATCAGCC GCTGCTCTGT GTGCGAGGCT CCGGCCGTGG CCATTGCCGT GCACAGCCAG 
GATACCTCTA TACCCCACTG CCCGGCTGGG TGGCGGAGTT TGTGGATCGG ATATTCATTC 
CTCATGCACA CTGCAGCCGG GGATGAAGGC GGTGGCCAGT CACTGGTGTC GCCGGGCAGC 
TGTCTAGAGG ACTTCCGTGC AACGCCATTT ATCGAGTGTA ACGGGGGCCG TGGTACCTGC 
CACTACTTCG CTAACAAGTA CAGCTTCTGG CTGACCACGA TCCCAGAGCA GAACTTCCAG 
AGCACACCAT CCGCTGACAC GCTCAAGGCT GGCCTCATCC GCACGCACAT CAGCCGCTGC 
CAAGTGTGCA TGAAGAATCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034062.3
CDS	356..5479
Translation	MDRVRFKASGPPLRGWLLLATVTVGLLAQSVLGGVKKLDVPCGGRDCSGGCQCYPEKGARGQPGAVGPQGYNGPPGLQGFPGLQGRKGDKGERGVPGPTGPKGDVGARGVSGFPGADGIPGHPGQGGPRGRPGYDGCNGTRGDAGPQGPSGSGGFPGLPGPQGPKGQKGEPYALSKEDRDKYRGEPGEPGLVGYQGPPGRPGPIGQMGPMGAPGRPGPPGPPGPKGQPGNRGLGFYGQKGEKGDIGQPGPNGIPSDITLVGPTTSTIHPDLYKGEKGDEGEQGIPGVISKGEEGIMGFPGIRGFPGLDGEKGVVGQKGSRGLDGFQGPSGPRGPKGERGEQGPPGPSVYSPHPSLAKGARGDPGFQGAHGEPGSRGEPGEPGTAGPPGPSVGDEDSMRGLPGEMGPKGFSGEPGSPARYLGPPGADGRPGPQGVPGPAGPPGPDGFLFGLKGSEGRVGYPGPSGFPGTRGQKGWKGEAGDCQCGQVIGGLPGLPGPKGFPGVNGELGKKGDQGDPGLHGIPGFPGFKGAPGVAGAPGPKGIKGDSRTITTKGERGQPGIPGVHGMKGDDGVPGRDGLDGFPGLPGPPGDGIKGPPGDAGLPGVPGTKGFPGDIGPPGQGLPGPKGERGFPGDAGLPGPPGFPGPPGPPGTPGQRDCDTGVKRPIGGGQQVVVQPGCIEGPTGSPGQPGPPGPTGAKGVRGMPGFPGASGEQGLKGFPGDPGREGFPGPPGFMGPRGSKGTTGLPGPDGPPGPIGLPGPAGPPGDRGIPGEVLGAQPGTRGDAGLPGQPGLKGLPGETGAPGFRGSQGMPGMPGLKGQPGFPGPSGQPGQSGPPGQHGFPGTPGREGPLGQPGSPGLGGLPGDRGEPGDPGVPGPVGMKGLSGDRGDAGMSGERGHPGSPGFKGMAGMPGIPGQKGDRGSPGMDGFQGMLGLKGRQGFPGTKGEAGFFGVPGLKGLPGEPGVKGNRGDRGPPGPPPLILPGMKDIKGEKGDEGPMGLKGYLGLKGIQGMPGVPGVSGFPGLPGRPGFIKGVKGDIGVPGTPGLPGFPGVSGPPGITGFPGFTGSRGEKGTPGVAGVFGETGPTGDFGDIGDTVDLPGSPGLKGERGITGIPGLKGFFGEKGAAGDIGFPGITGMAGAQGSPGLKGQTGFPGLTGLQGPQGEPGRIGIPGDKGDFGWPGVPGLPGFPGIRGISGLHGLPGTKGFPGSPGVDAHGDPGFPGPTGDRGDRGEANTLPGPVGVPGQKGERGTPGERGPAGSPGLQGFPGISPPSNISGSPGDVGAPGIFGLQGYQGPPGPPGPNALPGIKGDEGSSGAAGFPGQKGWVGDPGPQGQPGVLGLPGEKGPKGEQGFMGNTGPSGAVGDRGPKGPKGDQGFPGAPGSMGSPGIPGIPQKIAVQPGTLGPQGRRGLPGALGEIGPQGPPGDPGFRGAPGKAGPQGRGGVSAVPGFRGDQGPMGHQGPVGQEGEPGRPGSPGLPGMPGRSVSIGYLLVKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEEEIKPYISRCSVCEAPAVAIAVHSQDTSIPHCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYFANKYSFWLTTIPEQNFQSTPSADTLKAGLIRTHISRCQVCMKNL

Target ORF information:

RefSeq Version	NM_009932.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 2 (Col4a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009932.4

ORF Insert Sequence:

ATGGACCGAG TGCGGTTCAA AGCGTCTGGC CCTCCCCTGC GAGGGTGGCT GCTGCTAGCA 
ACTGTGACAG TGGGACTCCT GGCTCAGAGC GTCTTGGGGG GTGTGAAGAA GTTGGATGTG 
CCCTGCGGAG GGAGAGACTG CAGTGGGGGT TGCCAGTGCT ACCCGGAGAA AGGAGCAAGG 
GGTCAGCCAG GGGCAGTGGG CCCCCAGGGG TACAATGGCC CCCCAGGGTT GCAAGGATTC 
CCAGGACTAC AGGGCCGCAA AGGAGACAAG GGCGAACGGG GAGTTCCTGG ACCAACTGGA 
CCAAAAGGAG ATGTGGGAGC GAGAGGCGTC TCTGGATTCC CCGGTGCAGA TGGAATTCCT 
GGGCATCCAG GGCAAGGCGG ACCCCGAGGA AGGCCAGGCT ACGATGGTTG CAACGGAACC 
AGGGGAGACG CGGGTCCACA GGGGCCCTCT GGATCTGGGG GCTTCCCTGG CCTCCCTGGG 
CCCCAAGGAC CCAAGGGGCA GAAAGGCGAG CCTTACGCAC TCTCTAAAGA AGACCGTGAC 
AAATACCGGG GTGAACCTGG AGAGCCTGGC CTGGTTGGCT ACCAGGGACC TCCTGGCCGC 
CCAGGGCCCA TAGGACAGAT GGGTCCCATG GGAGCACCTG GAAGACCGGG ACCACCGGGA 
CCCCCTGGAC CCAAGGGACA ACCAGGCAAC CGAGGGCTCG GTTTTTACGG ACAGAAGGGT 
GAAAAGGGTG ACATAGGACA GCCAGGACCC AACGGGATCC CATCTGACAT CACACTTGTT 
GGGCCCACAA CATCAACGAT CCACCCGGAT CTGTACAAGG GTGAAAAAGG AGACGAAGGA 
GAGCAAGGGA TACCCGGCGT AATCTCAAAA GGCGAGGAAG GCATCATGGG GTTCCCAGGA 
ATACGGGGTT TTCCTGGCCT TGATGGAGAA AAAGGAGTCG TAGGACAGAA AGGAAGCAGA 
GGCCTGGATG GTTTCCAAGG CCCCAGTGGA CCTCGAGGAC CCAAGGGAGA ACGGGGGGAA 
CAAGGACCCC CAGGACCCTC TGTCTACTCG CCCCATCCAT CCCTGGCAAA AGGTGCCCGA 
GGTGACCCAG GATTCCAAGG TGCTCATGGG GAGCCAGGAA GCCGAGGCGA ACCGGGAGAG 
CCTGGAACCG CGGGTCCTCC AGGCCCATCC GTCGGAGATG AAGATTCAAT GAGAGGCCTT 
CCAGGGGAGA TGGGACCCAA AGGCTTCTCA GGAGAACCAG GGTCCCCTGC ACGCTATCTT 
GGCCCACCAG GAGCAGATGG AAGACCAGGT CCCCAAGGAG TCCCTGGACC TGCAGGCCCA 
CCTGGACCAG ATGGCTTCCT GTTTGGCCTG AAAGGATCAG AAGGGAGAGT GGGCTACCCG 
GGACCTTCAG GTTTCCCAGG AACACGAGGG CAGAAAGGAT GGAAAGGTGA AGCTGGAGAC 
TGCCAATGTG GCCAGGTCAT CGGGGGTCTT CCGGGACTGC CAGGACCCAA AGGTTTTCCC 
GGTGTCAATG GGGAGCTCGG AAAGAAAGGC GACCAAGGAG ACCCAGGCCT GCACGGTATC 
CCTGGGTTCC CAGGATTCAA GGGAGCCCCG GGCGTTGCTG GAGCTCCTGG ACCCAAAGGA 
ATAAAGGGAG ACTCTAGAAC AATCACCACC AAAGGGGAGC GAGGACAGCC AGGTATCCCT 
GGTGTACACG GAATGAAAGG TGATGACGGC GTCCCAGGAC GGGATGGGCT GGATGGATTC 
CCTGGCCTGC CTGGCCCCCC GGGCGATGGC ATCAAAGGCC CTCCAGGGGA TGCAGGTCTC 
CCTGGTGTAC CCGGAACCAA GGGCTTTCCA GGAGACATCG GTCCTCCAGG ACAGGGCTTA 
CCAGGTCCGA AAGGCGAGCG TGGTTTCCCT GGAGACGCTG GGTTGCCTGG ACCTCCGGGC 
TTCCCTGGTC CTCCAGGTCC CCCAGGAACC CCAGGACAGA GAGATTGTGA CACGGGTGTG 
AAAAGACCTA TCGGAGGCGG CCAGCAAGTG GTCGTTCAGC CAGGTTGCAT TGAAGGCCCC 
ACAGGATCAC CTGGCCAGCC AGGACCCCCA GGCCCCACAG GTGCCAAAGG CGTCAGAGGA 
ATGCCAGGAT TCCCAGGAGC TTCTGGAGAA CAAGGGCTCA AGGGCTTTCC AGGAGACCCA 
GGTCGAGAAG GCTTCCCAGG GCCCCCAGGG TTCATGGGAC CCCGAGGATC CAAAGGTACC 
ACAGGCCTCC CTGGCCCAGA TGGACCCCCA GGCCCCATTG GACTTCCAGG ACCAGCTGGA 
CCCCCTGGGG ACAGAGGGAT TCCAGGAGAA GTGCTAGGGG CCCAGCCTGG GACACGAGGA 
GATGCTGGAC TTCCAGGACA GCCTGGGCTG AAAGGGCTCC CAGGAGAAAC AGGAGCACCG 
GGATTCAGAG GAAGCCAAGG GATGCCCGGG ATGCCAGGGC TTAAAGGTCA GCCAGGCTTC 
CCAGGACCTT CGGGACAGCC AGGACAGTCT GGGCCCCCAG GACAACATGG ATTCCCAGGA 
ACTCCTGGCC GAGAGGGGCC TTTGGGGCAG CCAGGCTCCC CTGGCCTTGG AGGTTTGCCT 
GGAGACAGGG GCGAGCCTGG TGACCCTGGT GTCCCTGGCC CTGTGGGCAT GAAAGGTCTG 
TCTGGGGACA GAGGTGACGC CGGTATGTCA GGCGAGCGTG GTCACCCTGG AAGCCCTGGA 
TTTAAAGGAA TGGCAGGGAT GCCTGGTATC CCTGGGCAGA AAGGTGATCG AGGGTCGCCT 
GGAATGGACG GTTTCCAGGG CATGTTGGGA CTTAAGGGAA GACAGGGCTT CCCAGGGACC 
AAAGGAGAAG CTGGATTTTT TGGAGTTCCT GGTTTGAAGG GCCTGCCTGG CGAGCCAGGG 
GTTAAAGGAA ACCGAGGAGA CCGGGGGCCC CCAGGACCAC CTCCTCTCAT CCTGCCGGGG 
ATGAAGGACA TCAAGGGAGA AAAGGGAGAT GAAGGACCAA TGGGCCTGAA AGGGTATCTG 
GGCTTAAAAG GCATCCAAGG AATGCCCGGA GTCCCCGGAG TGTCTGGATT CCCTGGGCTA 
CCTGGAAGGC CTGGCTTCAT CAAAGGAGTC AAGGGAGACA TCGGAGTCCC TGGCACACCA 
GGCTTGCCGG GATTCCCTGG GGTGTCTGGC CCTCCTGGAA TTACCGGGTT TCCAGGATTC 
ACAGGCAGCC GGGGCGAGAA GGGTACTCCA GGAGTAGCAG GAGTTTTTGG CGAGACCGGC 
CCTACTGGGG ACTTTGGTGA CATTGGGGAC ACTGTGGACT TACCAGGGAG CCCAGGCCTG 
AAGGGGGAAC GGGGCATCAC GGGAATTCCA GGTCTCAAGG GATTCTTTGG AGAGAAAGGA 
GCTGCGGGTG ACATTGGATT TCCTGGGATA ACGGGCATGG CTGGAGCTCA GGGCTCTCCT 
GGACTTAAAG GACAGACAGG CTTTCCAGGG CTCACAGGGC TGCAAGGGCC ACAGGGAGAG 
CCTGGACGGA TCGGAATACC CGGTGACAAG GGTGATTTCG GCTGGCCAGG CGTACCAGGT 
CTACCAGGTT TTCCTGGGAT CCGGGGCATC AGCGGATTGC ACGGCCTGCC AGGCACCAAA 
GGCTTCCCTG GATCACCAGG TGTTGATGCT CATGGAGACC CAGGGTTCCC AGGCCCCACT 
GGAGACAGGG GTGACCGGGG AGAGGCCAAC ACACTTCCAG GCCCCGTGGG AGTTCCAGGG 
CAGAAAGGGG AGCGGGGAAC CCCAGGGGAA CGTGGCCCAG CTGGAAGCCC AGGACTTCAG 
GGTTTCCCTG GTATCTCTCC ACCATCCAAC ATCTCTGGGT CACCTGGTGA TGTAGGCGCA 
CCAGGAATAT TTGGCTTGCA AGGCTACCAA GGCCCTCCAG GACCACCTGG GCCAAACGCA 
CTTCCTGGAA TCAAAGGAGA TGAGGGGAGC TCTGGAGCTG CAGGATTCCC TGGACAGAAA 
GGATGGGTTG GGGACCCAGG GCCCCAGGGC CAGCCTGGTG TACTCGGTCT TCCGGGGGAG 
AAAGGGCCCA AGGGTGAACA AGGATTCATG GGCAACACCG GGCCCTCTGG GGCCGTGGGT 
GACAGAGGCC CCAAAGGACC CAAAGGCGAC CAAGGATTCC CAGGTGCTCC TGGCTCTATG 
GGGTCCCCAG GAATTCCTGG CATCCCCCAG AAGATTGCTG TCCAGCCTGG AACGCTGGGT 
CCCCAGGGCA GGAGAGGCCT TCCTGGGGCC CTGGGAGAGA TAGGGCCGCA GGGCCCTCCT 
GGAGATCCAG GCTTCCGTGG GGCCCCAGGC AAGGCTGGGC CTCAGGGCAG AGGTGGCGTG 
TCTGCTGTTC CAGGGTTCCG GGGAGACCAA GGGCCCATGG GACACCAGGG TCCAGTTGGC 
CAGGAAGGGG AGCCAGGCCG TCCAGGGAGC CCAGGCCTGC CAGGGATGCC AGGCCGCAGT 
GTGAGCATCG GCTACCTCCT GGTGAAGCAC AGCCAAACGG ACCAGGAACC CATGTGCCCT 
GTGGGCATGA ACAAGCTCTG GAGTGGGTAC AGCCTGCTAT ATTTTGAGGG CCAGGAGAAA 
GCGCACAACC AGGACCTAGG ACTGGCAGGC TCCTGCCTGG CACGCTTCAG CACCATGCCT 
TTCCTGTACT GCAATCCGGG TGACGTCTGC TACTATGCCA GCCGCAACGA CAAGTCCTAC 
TGGCTCTCCA CCACGGCCCC TCTGCCCATG ATGCCCGTGG CTGAGGAGGA AATCAAGCCC 
TACATCAGCC GCTGCTCTGT GTGCGAGGCT CCGGCCGTGG CCATTGCCGT GCACAGCCAG 
GATACCTCTA TACCCCACTG CCCGGCTGGG TGGCGGAGTT TGTGGATCGG ATATTCATTC 
CTCATGCACA CTGCAGCCGG GGATGAAGGC GGTGGCCAGT CACTGGTGTC GCCGGGCAGC 
TGTCTAGAGG ACTTCCGTGC AACGCCATTT ATCGAGTGTA ACGGGGGCCG TGGTACCTGC 
CACTACTTCG CTAACAAGTA CAGCTTCTGG CTGACCACGA TCCCAGAGCA GAACTTCCAG 
AGCACACCAT CCGCTGACAC GCTCAAGGCT GGCCTCATCC GCACGCACAT CAGCCGCTGC 
CAAGTGTGCA TGAAGAATCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lhx8 ablation leads to massive autophagy of mouse oocytes associated with DNA damage.
Biology of reproduction(2018 Jan)
Pelosi E,D'Ignazio L,Michel M,Beyer M,Thompson K,Forabosco A,Schlessinger D

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

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Col4a2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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