Col4a1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col4a1
Entrez Gene ID	33727
Full Name	Collagen type IV alpha 1
Synonyms	CG25C,CG4145,CT12803,Cg25C,Cgc25,Cgc25C,Col IV alpha(1),Coll,Coll IV,DCg1,Dcg,Dcg1,Dmel\CG4145,alpha(IV)1/Cg25C,alpha1,cg25C,cg25c,col4a1,dcg1,gp125
General protein information	Preferred Names Collagen type IV alpha 1 Names CG4145 gene product from transcript CG4145-RB CG4145-PA CG4145-PB CG4145-PC Col4a1-PA Col4a1-PB Col4a1-PC collagen collagen IV collagen type IV collagen type IV alpha 1 collagenIV a1 laminin alpha3,5 preprocollagen alpha1(IV) procollagen IV type IV collagen type IV collagen, alpha-chain
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 25C1-25C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_164615.2	NP_723044.1	collagen type IV alpha 1, isoform A
NM_164617.2	NP_723046.1	collagen type IV alpha 1, isoform C
NM_164616.2	NP_723045.1	collagen type IV alpha 1, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL4A2	XP_001136859.1
Canis lupus familiaris (dog)	COL4A2	XP_534183.3
Rattus norvegicus (Norway rat)	Col4a2	XP_006253538.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009200	XP_319976.3
	COL4A2	XP_002800878.1
Drosophila melanogaster (fruit fly)	Cg25C	NP_723046.1
Caenorhabditis elegans (roundworm)	emb-9	NP_001022662.1
Bos taurus (cattle)	COL4A2	XP_002692057.2
Mus musculus (house mouse)	Col4a2	NP_034062.3
Gallus gallus (chicken)	COL4A2	NP_001155862.1
Danio rerio (zebrafish)	col4a2	XP_687811.6
Caenorhabditis elegans (roundworm)	let-2	NP_510663.1
Xenopus tropicalis (tropical clawed frog)	col4a2	XP_004912045.1
Homo sapiens (human)	COL4A2	NP_001837.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results of phenotypic features of Drosophila col4a1 mutants confirmed an essential role for type IV collagen in maintaining epithelial integrity, gut morphology and intestinal function and suggest that aberrant structure and function of the COL4A1 protein may also be a significant factor in modulating immunity.
Title: Drosophila type IV collagen mutation associates with immune system activation and intestinal dysfunction.

Data show that laminin beta and collagen IV alpha1 were involved in cardiac and muscular abnormalities.
Title: Distinct functions of the laminin ? LN domain and collagen IV during cardiac extracellular matrix formation and stabilization of alary muscle attachments revealed by EMS mutagenesis in Drosophila.

collagen IV functions to immobilize free Dpp in the Drosophila embryo
Title: Multistep molecular mechanism for bone morphogenetic protein extracellular transport in the Drosophila embryo.

Drosophila basement membrane collagen col4a1 mutations cause severe myopathy.
Title: Drosophila basement membrane collagen col4a1 mutations cause severe myopathy.

Data suggest that collagen IV acts to enhance ligand presentation to tubule cell receptors and is required to ensure ligand-mediated activation of the BMP pathway.
Title: Hemocyte-secreted type IV collagen enhances BMP signaling to guide renal tubule morphogenesis in Drosophila.

The following Col4a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col4a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16240	NM_164617.2 Latest version!	Drosophila melanogaster collagen type IV alpha 1, transcript variant C (Col4a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa16240	NM_164616.2 Latest version!	Drosophila melanogaster collagen type IV alpha 1, transcript variant B (Col4a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa16240	NM_164615.2 Latest version!	Drosophila melanogaster collagen type IV alpha 1, transcript variant A (Col4a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col4a1 ( NM_164615.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723044.1 Drosophila melanogaster collagen type IV alpha 1, transcript variant A (Col4a1), mRNA.

Price & Availability↑

CloneID	OFa16240
Clone ID Related Accession (Same CDS sequence)	NM_164615.2 , NM_164617.2 , NM_164616.2
Accession Version	NM_164615.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	collagen type IV alpha 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164615.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCCCT TCTGGAAGCG GCTGCTATAC GCCGCTGTGA TCGCGGGAGC GTTAGTCGGT 
GCCGACGCTC AATTTTGGAA GACGGCTGGT ACGGCCGGTT CGATTCAGGA CTCCGTGAAA 
CACTACAATC GAAATGAACC CAAGTTCCCA ATCGACGACA GTTACGACAT TGTAGACTCT 
GCCGGCGTGG CGCGCGGCGA TCTGCCGCCC AAGAATTGTA CGGCCGGATA TGCGGGCTGT 
GTGCCCAAGT GCATAGCGGA GAAGGGCAAC CGTGGTCTGC CGGGCCCTCT TGGACCCACG 
GGATTGAAGG GCGAAATGGG TTTCCCTGGC ATGGAGGGAC CATCTGGTGA CAAGGGTCAG 
AAGGGTGATC CCGGCCCATA CGGACAGCGT GGTGATAAGG GTGAGCGCGG ATCGCCTGGT 
CTTCATGGTC AGGCTGGTGT ACCCGGAGTC CAGGGACCCG CCGGCAATCC CGGAGCCCCT 
GGTATCAACG GTAAAGACGG TTGCGACGGA CAGGATGGTA TTCCTGGCTT GGAGGGTCTA 
TCCGGAATGC CCGGACCTCG CGGATATGCC GGCCAGCTTG GCAGCAAGGG CGAGAAGGGT 
GAACCGGCAA AGGAGAACGG TGATTACGCG AAGGGCGAGA AGGGTGAGCC CGGTTGGAGG 
GGGACTGCCG GTTTGGCTGG ACCACAAGGA TTTCCTGGAG AAAAGGGCGA GCGCGGCGAC 
AGTGGACCTT ACGGAGCCAA AGGACCCCGG GGTGAGCACG GTCTGAAGGG AGAGAAGGGT 
GCCTCCTGCT ACGGACCCAT GAAGCCTGGT GCACCGGGTA TCAAGGGCGA GAAGGGTGAG 
CCCGCGTCCT CGTTTCCCGT CAAACCGACC CACACGGTGA TGGGACCTCG CGGCGATATG 
GGACAGAAGG GAGAGCCTGG CCTAGTTGGC CGCAAGGGTG AGCCCGGACC TGAAGGCGAC 
ACTGGACTCG ATGGACAGAA GGGCGAGAAG GGTCTGCCCG GCGGCCCAGG CGATCGCGGT 
CGCCAAGGTA ACTTTGGACC CCCAGGATCT ACAGGACAAA AGGGAGATCG TGGCGAGCCG 
GGCCTTAATG GTCTGCCCGG TAATCCCGGA CAGAAGGGTG AGCCAGGACG TGCTGGAGCG 
ACAGGTAAGC CTGGTCTGCT CGGTCCTCCG GGACCGCCAG GCGGTGGCCG TGGAACACCA 
GGACCCCCGG GACCCAAAGG ACCCCGCGGC TATGTTGGCG CACCTGGACC CCAGGGATTA 
AACGGAGTTG ATGGACTACC GGGTCCTCAG GGATACAATG GACAAAAGGG AGGTGCTGGT 
CTGCCCGGTC GCCCCGGCAA CGAGGGACCT CCTGGCAAAA AGGGAGAAAA GGGAACCGCA 
GGACTTAATG GACCAAAGGG ATCCATCGGA CCCATTGGAC ACCCAGGACC ACCGGGACCA 
GAGGGACAGA AGGGTGACGC CGGTTTGCCC GGTTATGGCA TTCAAGGATC TAAGGGAGAT 
GCTGGCATAC CTGGTTATCC CGGACTAAAG GGTAGCAAGG GAGAGCGCGG CTTCAAGGGC 
AATGCTGGTG CTCCCGGTGA CTCCAAGCTG GGTCGTCCTG GAACTCCCGG TGCCGCTGGT 
GCTCCTGGAC AAAAGGGAGA CGCTGGTCGT CCCGGCACTC CTGGCCAAAA GGGAGACATG 
GGTATCAAGG GTGACGTCGG CGGCAAATGC TCATCGTGCA GGGCCGGACC AAAGGGTGAT 
AAGGGAACGA GCGGACTGCC TGGAATTCCC GGAAAGGATG GCGCACGAGG ACCGCCTGGA 
GAGCGCGGAT ATCCCGGAGA GCGTGGACAC GATGGAATCA ACGGACAAAC TGGACCGCCT 
GGTGAGAAGG GAGAGGACGG TCGCACTGGT CTTCCCGGAG CAACTGGAGA GCCTGGCAAA 
CCTGCTCTGT GCGATTTGAG TTTGATTGAG CCATTGAAGG GTGACAAGGG TTACCCTGGT 
GCGCCAGGTG CAAAGGGCGT GCAAGGATTC AAGGGAGCAG AAGGTCTGCC TGGTATCCCT 
GGACCCAAAG GAGAATTCGG TTTCAAGGGT GAGAAGGGTT TGAGCGGAGC ACCCGGCAAC 
GACGGAACAC CCGGACGCGC TGGGCGGGAC GGATACCCCG GAATTCCCGG TCAATCCATC 
AAGGGCGAGC CAGGCTTCCA TGGAAGGGAC GGAGCAAAGG GCGACAAGGG ATCATTTGGC 
CGAAGCGGCG AGAAGGGAGA GCCCGGTAGC TGTGCGCTTG ACGAAATTAA GATGCCCGCC 
AAGGGTAACA AGGGTGAGCC CGGCCAAACC GGCATGCCAG GACCTCCGGG AGAAGACGGC 
AGCCCGGGAG AGAGGGGCTA TACCGGATTG AAGGGCAACA CTGGACCACA GGGACCTCCT 
GGCGTTGAAG GACCCCGCGG CTTGAATGGA CCTCGCGGTG AAAAGGGCAA CCAGGGCGCT 
GTCGGAGTAC CTGGTAATCC TGGCAAGGAC GGCCTTCGCG GCATTCCCGG ACGCAATGGA 
CAGCCTGGAC CGAGGGGAGA GCCTGGTATT TCGAGACCCG GCCCTATGGG CCCACCCGGT 
CTCAATGGTC TGCAAGGTGA GAAGGGCGAC CGTGGTCCAA CCGGACCCAT TGGTTTTCCC 
GGTGCCGATG GCAGTGTGGG ATATCCTGGA GATAGAGGCG ATGCCGGTCT GCCCGGAGTA 
TCTGGACGTC CCGGAATTGT TGGTGAGAAG GGAGACGTGG GCCCGATCGG ACCCGCTGGT 
GTTGCCGGAC CTCCTGGTGT TCCTGGTATT GATGGTGTGC GTGGACGTGA TGGCGCCAAG 
GGTGAGCCCG GCAGTCCCGG ATTGGTCGGC ATGCCCGGTA ACAAAGGTGA CCGTGGTGCT 
CCTGGAAATG ACGGACCCAA GGGCTTTGCT GGCGTTACTG GTGCTCCCGG AAAGCGCGGA 
CCTGCTGGTA TTCCCGGAGT TTCCGGTGCC AAGGGTGACA AGGGCGCTAC TGGCTTGACT 
GGCAACGATG GACCTGTGGG AGGCCGCGGT CCTCCAGGTG CTCCTGGACT GATGGGCATT 
AAGGGTGACC AAGGATTGGC AGGCGCCCCT GGACAACAAG GACTGGACGG TATGCCTGGC 
GAAAAGGGTA ACCAAGGATT CCCCGGTCTG GATGGACCTC CTGGTTTGCC TGGAGATGCC 
TCCGAGAAAG GACAAAAGGG TGAACCCGGT CCATCCGGAC TCCGCGGCGA TACAGGTCCG 
GCCGGAACGC CCGGTTGGCC AGGAGAGAAG GGTTTGCCCG GTCTGGCTGT TCACGGTCGT 
GCTGGTCCGC CAGGCGAGAA GGGTGACCAG GGACGCAGTG GAATCGATGG ACGAGATGGA 
ATTAACGGCG AGAAGGGTGA ACAAGGTCTG CAGGGCGTTT GGGGCCAGCC TGGCGAGAAG 
GGATCTGTCG GCGCACCTGG TATTCCTGGT GCTCCCGGAA TGGATGGTTT GCCCGGCGCT 
GCTGGTGCTC CTGGTGCTGT TGGCTATCCT GGTGATCGCG GTGACAAGGG AGAGCCTGGT 
CTATCTGGTC TGCCCGGACT CAAGGGTGAG ACTGGACCCG TTGGACTGCA GGGCTTCACC 
GGTGCTCCTG GCCCTAAGGG TGAGCGCGGT ATTCGTGGTC AGCCCGGTCT TCCGGCCACC 
GTTCCCGACA TCCGTGGTGA TAAGGGATCC CAGGGCGAGC GCGGCTACAC TGGCGAGAAG 
GGCGAGCAAG GCGAACGAGG CTTGACTGGT CCTGCTGGCG TCGCTGGAGC AAAGGGAGAT 
CGCGGATTGC AGGGACCACC AGGTGCAAGC GGATTGAACG GCATTCCCGG AGCCAAGGGA 
GACATTGGTC CAAGAGGCGA GATCGGTTAT CCAGGAGTTA CCATTAAGGG CGAGAAGGGT 
CTGCCCGGTC GCCCAGGCAG AAACGGACGT CAAGGTCTTA TTGGAGCACC CGGCTTAATT 
GGAGAACGTG GTCTGCCTGG CTTGGCCGGA GAGCCCGGCC TCGTGGGCCT GCCTGGACCC 
ATTGGACCAG CTGGCAGCAA GGGAGAGCGT GGTCTCGCCG GCAGTCCCGG ACAACCAGGA 
CAGGATGGCT TCCCCGGCGC ACCTGGATTG AAGGGAGATA CTGGACCGCA GGGCTTTAAG 
GGCGAACGTG GTCTGAATGG CTTCGAGGGA CAAAAGGGAG ACAAGGGTGA CCGAGGACTC 
CAAGGACCGT CTGGACTGCC CGGCTTGGTT GGACAGAAGG GAGATACCGG CTACCCTGGC 
TTAAATGGAA ACGATGGACC TGTCGGAGCT CCTGGCGAGC GCGGCTTCAC CGGGCCCAAG 
GGACGCGATG GACGCGACGG AACACCAGGT CTGCCTGGAC AGAAGGGTGA ACCAGGAATG 
CTGCCACCAC CAGGACCCAA GGGCGAACCT GGTCAGCCGG GACGCAATGG ACCTAAGGGA 
GAGCCCGGAC GTCCGGGAGA GCGTGGCTTG ATTGGCATCC AGGGTGAGCG TGGCGAAAAG 
GGTGAGCGCG GCCTGATCGG TGAGACTGGT AACGTGGGAC GACCCGGACC CAAGGGAGAT 
CGCGGAGAGC CAGGCGAGAG GGGATATGAG GGCGCCATTG GTTTGATCGG CCAGAAGGGT 
GAGCCCGGTG CTCCAGCCCC CGCTGCTCTG GACTATCTCA CCGGTATCCT GATTACGCGA 
CACAGTCAAT CGGAAACGGT GCCCGCTTGC TCGGCTGGAC ACACGGAACT GTGGACGGGT 
TACTCCCTGT TGTACGTCGA TGGCAATGAC TATGCCCACA ACCAGGACCT TGGATCCCCC 
GGATCCTGTG TGCCACGCTT CTCAACGCTG CCCGTACTGT CGTGTGGTCA GAATAACGTC 
TGCAACTACG CCTCCAGAAA TGACAAGACC TTCTGGCTGA CAACCAACGC CGCCATTCCG 
ATGATGCCCG TTGAAAACAT CGAGATCCGC CAGTACATCT CACGTTGCGT CGTTTGTGAG 
GCGCCGGCTA ATGTGATCGC CGTGCACAGT CAAACGATAG AGGTGCCCGA CTGTCCGAAT 
GGCTGGGAGG GTCTCTGGAT TGGCTACAGT TTCCTCATGC ACACTGCCGT GGGCAACGGT 
GGCGGTGGAC AGGCGCTGCA ATCGCCTGGC TCCTGTTTGG AGGACTTCCG TGCAACGCCC 
TTCATCGAGT GCAACGGCGC CAAGGGCACG TGCCACTTCT ATGAGACGAT GACAAGCTTC 
TGGATGTACA ACCTGGAGTC CTCACAGCCG TTCGAGAGGC CACAGCAGCA GACGATCAAG 
GCCGGCGAGC GGCAGTCGCA TGTGTCCAGG TGCCAGGTGT GCATGAAGAA CTCCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723044.1
CDS	284..5623
Translation	MLPFWKRLLYAAVIAGALVGADAQFWKTAGTAGSIQDSVKHYNRNEPKFPIDDSYDIVDSAGVARGDLPPKNCTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIGLIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQDLGSPGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPVENIEIRQYISRCVVCEAPANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECNGAKGTCHFYETMTSFWMYNLESSQPFERPQQQTIKAGERQSHVSRCQVCMKNSS

Target ORF information:

RefSeq Version	NM_164615.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster collagen type IV alpha 1, transcript variant A (Col4a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164615.2

ORF Insert Sequence:

ATGTTGCCCT TCTGGAAGCG GCTGCTATAC GCCGCTGTGA TCGCGGGAGC GTTAGTCGGT 
GCCGACGCTC AATTTTGGAA GACGGCTGGT ACGGCCGGTT CGATTCAGGA CTCCGTGAAA 
CACTACAATC GAAATGAACC CAAGTTCCCA ATCGACGACA GTTACGACAT TGTAGACTCT 
GCCGGCGTGG CGCGCGGCGA TCTGCCGCCC AAGAATTGTA CGGCCGGATA TGCGGGCTGT 
GTGCCCAAGT GCATAGCGGA GAAGGGCAAC CGTGGTCTGC CGGGCCCTCT TGGACCCACG 
GGATTGAAGG GCGAAATGGG TTTCCCTGGC ATGGAGGGAC CATCTGGTGA CAAGGGTCAG 
AAGGGTGATC CCGGCCCATA CGGACAGCGT GGTGATAAGG GTGAGCGCGG ATCGCCTGGT 
CTTCATGGTC AGGCTGGTGT ACCCGGAGTC CAGGGACCCG CCGGCAATCC CGGAGCCCCT 
GGTATCAACG GTAAAGACGG TTGCGACGGA CAGGATGGTA TTCCTGGCTT GGAGGGTCTA 
TCCGGAATGC CCGGACCTCG CGGATATGCC GGCCAGCTTG GCAGCAAGGG CGAGAAGGGT 
GAACCGGCAA AGGAGAACGG TGATTACGCG AAGGGCGAGA AGGGTGAGCC CGGTTGGAGG 
GGGACTGCCG GTTTGGCTGG ACCACAAGGA TTTCCTGGAG AAAAGGGCGA GCGCGGCGAC 
AGTGGACCTT ACGGAGCCAA AGGACCCCGG GGTGAGCACG GTCTGAAGGG AGAGAAGGGT 
GCCTCCTGCT ACGGACCCAT GAAGCCTGGT GCACCGGGTA TCAAGGGCGA GAAGGGTGAG 
CCCGCGTCCT CGTTTCCCGT CAAACCGACC CACACGGTGA TGGGACCTCG CGGCGATATG 
GGACAGAAGG GAGAGCCTGG CCTAGTTGGC CGCAAGGGTG AGCCCGGACC TGAAGGCGAC 
ACTGGACTCG ATGGACAGAA GGGCGAGAAG GGTCTGCCCG GCGGCCCAGG CGATCGCGGT 
CGCCAAGGTA ACTTTGGACC CCCAGGATCT ACAGGACAAA AGGGAGATCG TGGCGAGCCG 
GGCCTTAATG GTCTGCCCGG TAATCCCGGA CAGAAGGGTG AGCCAGGACG TGCTGGAGCG 
ACAGGTAAGC CTGGTCTGCT CGGTCCTCCG GGACCGCCAG GCGGTGGCCG TGGAACACCA 
GGACCCCCGG GACCCAAAGG ACCCCGCGGC TATGTTGGCG CACCTGGACC CCAGGGATTA 
AACGGAGTTG ATGGACTACC GGGTCCTCAG GGATACAATG GACAAAAGGG AGGTGCTGGT 
CTGCCCGGTC GCCCCGGCAA CGAGGGACCT CCTGGCAAAA AGGGAGAAAA GGGAACCGCA 
GGACTTAATG GACCAAAGGG ATCCATCGGA CCCATTGGAC ACCCAGGACC ACCGGGACCA 
GAGGGACAGA AGGGTGACGC CGGTTTGCCC GGTTATGGCA TTCAAGGATC TAAGGGAGAT 
GCTGGCATAC CTGGTTATCC CGGACTAAAG GGTAGCAAGG GAGAGCGCGG CTTCAAGGGC 
AATGCTGGTG CTCCCGGTGA CTCCAAGCTG GGTCGTCCTG GAACTCCCGG TGCCGCTGGT 
GCTCCTGGAC AAAAGGGAGA CGCTGGTCGT CCCGGCACTC CTGGCCAAAA GGGAGACATG 
GGTATCAAGG GTGACGTCGG CGGCAAATGC TCATCGTGCA GGGCCGGACC AAAGGGTGAT 
AAGGGAACGA GCGGACTGCC TGGAATTCCC GGAAAGGATG GCGCACGAGG ACCGCCTGGA 
GAGCGCGGAT ATCCCGGAGA GCGTGGACAC GATGGAATCA ACGGACAAAC TGGACCGCCT 
GGTGAGAAGG GAGAGGACGG TCGCACTGGT CTTCCCGGAG CAACTGGAGA GCCTGGCAAA 
CCTGCTCTGT GCGATTTGAG TTTGATTGAG CCATTGAAGG GTGACAAGGG TTACCCTGGT 
GCGCCAGGTG CAAAGGGCGT GCAAGGATTC AAGGGAGCAG AAGGTCTGCC TGGTATCCCT 
GGACCCAAAG GAGAATTCGG TTTCAAGGGT GAGAAGGGTT TGAGCGGAGC ACCCGGCAAC 
GACGGAACAC CCGGACGCGC TGGGCGGGAC GGATACCCCG GAATTCCCGG TCAATCCATC 
AAGGGCGAGC CAGGCTTCCA TGGAAGGGAC GGAGCAAAGG GCGACAAGGG ATCATTTGGC 
CGAAGCGGCG AGAAGGGAGA GCCCGGTAGC TGTGCGCTTG ACGAAATTAA GATGCCCGCC 
AAGGGTAACA AGGGTGAGCC CGGCCAAACC GGCATGCCAG GACCTCCGGG AGAAGACGGC 
AGCCCGGGAG AGAGGGGCTA TACCGGATTG AAGGGCAACA CTGGACCACA GGGACCTCCT 
GGCGTTGAAG GACCCCGCGG CTTGAATGGA CCTCGCGGTG AAAAGGGCAA CCAGGGCGCT 
GTCGGAGTAC CTGGTAATCC TGGCAAGGAC GGCCTTCGCG GCATTCCCGG ACGCAATGGA 
CAGCCTGGAC CGAGGGGAGA GCCTGGTATT TCGAGACCCG GCCCTATGGG CCCACCCGGT 
CTCAATGGTC TGCAAGGTGA GAAGGGCGAC CGTGGTCCAA CCGGACCCAT TGGTTTTCCC 
GGTGCCGATG GCAGTGTGGG ATATCCTGGA GATAGAGGCG ATGCCGGTCT GCCCGGAGTA 
TCTGGACGTC CCGGAATTGT TGGTGAGAAG GGAGACGTGG GCCCGATCGG ACCCGCTGGT 
GTTGCCGGAC CTCCTGGTGT TCCTGGTATT GATGGTGTGC GTGGACGTGA TGGCGCCAAG 
GGTGAGCCCG GCAGTCCCGG ATTGGTCGGC ATGCCCGGTA ACAAAGGTGA CCGTGGTGCT 
CCTGGAAATG ACGGACCCAA GGGCTTTGCT GGCGTTACTG GTGCTCCCGG AAAGCGCGGA 
CCTGCTGGTA TTCCCGGAGT TTCCGGTGCC AAGGGTGACA AGGGCGCTAC TGGCTTGACT 
GGCAACGATG GACCTGTGGG AGGCCGCGGT CCTCCAGGTG CTCCTGGACT GATGGGCATT 
AAGGGTGACC AAGGATTGGC AGGCGCCCCT GGACAACAAG GACTGGACGG TATGCCTGGC 
GAAAAGGGTA ACCAAGGATT CCCCGGTCTG GATGGACCTC CTGGTTTGCC TGGAGATGCC 
TCCGAGAAAG GACAAAAGGG TGAACCCGGT CCATCCGGAC TCCGCGGCGA TACAGGTCCG 
GCCGGAACGC CCGGTTGGCC AGGAGAGAAG GGTTTGCCCG GTCTGGCTGT TCACGGTCGT 
GCTGGTCCGC CAGGCGAGAA GGGTGACCAG GGACGCAGTG GAATCGATGG ACGAGATGGA 
ATTAACGGCG AGAAGGGTGA ACAAGGTCTG CAGGGCGTTT GGGGCCAGCC TGGCGAGAAG 
GGATCTGTCG GCGCACCTGG TATTCCTGGT GCTCCCGGAA TGGATGGTTT GCCCGGCGCT 
GCTGGTGCTC CTGGTGCTGT TGGCTATCCT GGTGATCGCG GTGACAAGGG AGAGCCTGGT 
CTATCTGGTC TGCCCGGACT CAAGGGTGAG ACTGGACCCG TTGGACTGCA GGGCTTCACC 
GGTGCTCCTG GCCCTAAGGG TGAGCGCGGT ATTCGTGGTC AGCCCGGTCT TCCGGCCACC 
GTTCCCGACA TCCGTGGTGA TAAGGGATCC CAGGGCGAGC GCGGCTACAC TGGCGAGAAG 
GGCGAGCAAG GCGAACGAGG CTTGACTGGT CCTGCTGGCG TCGCTGGAGC AAAGGGAGAT 
CGCGGATTGC AGGGACCACC AGGTGCAAGC GGATTGAACG GCATTCCCGG AGCCAAGGGA 
GACATTGGTC CAAGAGGCGA GATCGGTTAT CCAGGAGTTA CCATTAAGGG CGAGAAGGGT 
CTGCCCGGTC GCCCAGGCAG AAACGGACGT CAAGGTCTTA TTGGAGCACC CGGCTTAATT 
GGAGAACGTG GTCTGCCTGG CTTGGCCGGA GAGCCCGGCC TCGTGGGCCT GCCTGGACCC 
ATTGGACCAG CTGGCAGCAA GGGAGAGCGT GGTCTCGCCG GCAGTCCCGG ACAACCAGGA 
CAGGATGGCT TCCCCGGCGC ACCTGGATTG AAGGGAGATA CTGGACCGCA GGGCTTTAAG 
GGCGAACGTG GTCTGAATGG CTTCGAGGGA CAAAAGGGAG ACAAGGGTGA CCGAGGACTC 
CAAGGACCGT CTGGACTGCC CGGCTTGGTT GGACAGAAGG GAGATACCGG CTACCCTGGC 
TTAAATGGAA ACGATGGACC TGTCGGAGCT CCTGGCGAGC GCGGCTTCAC CGGGCCCAAG 
GGACGCGATG GACGCGACGG AACACCAGGT CTGCCTGGAC AGAAGGGTGA ACCAGGAATG 
CTGCCACCAC CAGGACCCAA GGGCGAACCT GGTCAGCCGG GACGCAATGG ACCTAAGGGA 
GAGCCCGGAC GTCCGGGAGA GCGTGGCTTG ATTGGCATCC AGGGTGAGCG TGGCGAAAAG 
GGTGAGCGCG GCCTGATCGG TGAGACTGGT AACGTGGGAC GACCCGGACC CAAGGGAGAT 
CGCGGAGAGC CAGGCGAGAG GGGATATGAG GGCGCCATTG GTTTGATCGG CCAGAAGGGT 
GAGCCCGGTG CTCCAGCCCC CGCTGCTCTG GACTATCTCA CCGGTATCCT GATTACGCGA 
CACAGTCAAT CGGAAACGGT GCCCGCTTGC TCGGCTGGAC ACACGGAACT GTGGACGGGT 
TACTCCCTGT TGTACGTCGA TGGCAATGAC TATGCCCACA ACCAGGACCT TGGATCCCCC 
GGATCCTGTG TGCCACGCTT CTCAACGCTG CCCGTACTGT CGTGTGGTCA GAATAACGTC 
TGCAACTACG CCTCCAGAAA TGACAAGACC TTCTGGCTGA CAACCAACGC CGCCATTCCG 
ATGATGCCCG TTGAAAACAT CGAGATCCGC CAGTACATCT CACGTTGCGT CGTTTGTGAG 
GCGCCGGCTA ATGTGATCGC CGTGCACAGT CAAACGATAG AGGTGCCCGA CTGTCCGAAT 
GGCTGGGAGG GTCTCTGGAT TGGCTACAGT TTCCTCATGC ACACTGCCGT GGGCAACGGT 
GGCGGTGGAC AGGCGCTGCA ATCGCCTGGC TCCTGTTTGG AGGACTTCCG TGCAACGCCC 
TTCATCGAGT GCAACGGCGC CAAGGGCACG TGCCACTTCT ATGAGACGAT GACAAGCTTC 
TGGATGTACA ACCTGGAGTC CTCACAGCCG TTCGAGAGGC CACAGCAGCA GACGATCAAG 
GCCGGCGAGC GGCAGTCGCA TGTGTCCAGG TGCCAGGTGT GCATGAAGAA CTCCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a1 ( NM_164617.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723046.1 Drosophila melanogaster collagen type IV alpha 1, transcript variant C (Col4a1), mRNA.

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CloneID	OFa16240
Clone ID Related Accession (Same CDS sequence)	NM_164615.2 , NM_164617.2 , NM_164616.2
Accession Version	NM_164617.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	collagen type IV alpha 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164617.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCCCT TCTGGAAGCG GCTGCTATAC GCCGCTGTGA TCGCGGGAGC GTTAGTCGGT 
GCCGACGCTC AATTTTGGAA GACGGCTGGT ACGGCCGGTT CGATTCAGGA CTCCGTGAAA 
CACTACAATC GAAATGAACC CAAGTTCCCA ATCGACGACA GTTACGACAT TGTAGACTCT 
GCCGGCGTGG CGCGCGGCGA TCTGCCGCCC AAGAATTGTA CGGCCGGATA TGCGGGCTGT 
GTGCCCAAGT GCATAGCGGA GAAGGGCAAC CGTGGTCTGC CGGGCCCTCT TGGACCCACG 
GGATTGAAGG GCGAAATGGG TTTCCCTGGC ATGGAGGGAC CATCTGGTGA CAAGGGTCAG 
AAGGGTGATC CCGGCCCATA CGGACAGCGT GGTGATAAGG GTGAGCGCGG ATCGCCTGGT 
CTTCATGGTC AGGCTGGTGT ACCCGGAGTC CAGGGACCCG CCGGCAATCC CGGAGCCCCT 
GGTATCAACG GTAAAGACGG TTGCGACGGA CAGGATGGTA TTCCTGGCTT GGAGGGTCTA 
TCCGGAATGC CCGGACCTCG CGGATATGCC GGCCAGCTTG GCAGCAAGGG CGAGAAGGGT 
GAACCGGCAA AGGAGAACGG TGATTACGCG AAGGGCGAGA AGGGTGAGCC CGGTTGGAGG 
GGGACTGCCG GTTTGGCTGG ACCACAAGGA TTTCCTGGAG AAAAGGGCGA GCGCGGCGAC 
AGTGGACCTT ACGGAGCCAA AGGACCCCGG GGTGAGCACG GTCTGAAGGG AGAGAAGGGT 
GCCTCCTGCT ACGGACCCAT GAAGCCTGGT GCACCGGGTA TCAAGGGCGA GAAGGGTGAG 
CCCGCGTCCT CGTTTCCCGT CAAACCGACC CACACGGTGA TGGGACCTCG CGGCGATATG 
GGACAGAAGG GAGAGCCTGG CCTAGTTGGC CGCAAGGGTG AGCCCGGACC TGAAGGCGAC 
ACTGGACTCG ATGGACAGAA GGGCGAGAAG GGTCTGCCCG GCGGCCCAGG CGATCGCGGT 
CGCCAAGGTA ACTTTGGACC CCCAGGATCT ACAGGACAAA AGGGAGATCG TGGCGAGCCG 
GGCCTTAATG GTCTGCCCGG TAATCCCGGA CAGAAGGGTG AGCCAGGACG TGCTGGAGCG 
ACAGGTAAGC CTGGTCTGCT CGGTCCTCCG GGACCGCCAG GCGGTGGCCG TGGAACACCA 
GGACCCCCGG GACCCAAAGG ACCCCGCGGC TATGTTGGCG CACCTGGACC CCAGGGATTA 
AACGGAGTTG ATGGACTACC GGGTCCTCAG GGATACAATG GACAAAAGGG AGGTGCTGGT 
CTGCCCGGTC GCCCCGGCAA CGAGGGACCT CCTGGCAAAA AGGGAGAAAA GGGAACCGCA 
GGACTTAATG GACCAAAGGG ATCCATCGGA CCCATTGGAC ACCCAGGACC ACCGGGACCA 
GAGGGACAGA AGGGTGACGC CGGTTTGCCC GGTTATGGCA TTCAAGGATC TAAGGGAGAT 
GCTGGCATAC CTGGTTATCC CGGACTAAAG GGTAGCAAGG GAGAGCGCGG CTTCAAGGGC 
AATGCTGGTG CTCCCGGTGA CTCCAAGCTG GGTCGTCCTG GAACTCCCGG TGCCGCTGGT 
GCTCCTGGAC AAAAGGGAGA CGCTGGTCGT CCCGGCACTC CTGGCCAAAA GGGAGACATG 
GGTATCAAGG GTGACGTCGG CGGCAAATGC TCATCGTGCA GGGCCGGACC AAAGGGTGAT 
AAGGGAACGA GCGGACTGCC TGGAATTCCC GGAAAGGATG GCGCACGAGG ACCGCCTGGA 
GAGCGCGGAT ATCCCGGAGA GCGTGGACAC GATGGAATCA ACGGACAAAC TGGACCGCCT 
GGTGAGAAGG GAGAGGACGG TCGCACTGGT CTTCCCGGAG CAACTGGAGA GCCTGGCAAA 
CCTGCTCTGT GCGATTTGAG TTTGATTGAG CCATTGAAGG GTGACAAGGG TTACCCTGGT 
GCGCCAGGTG CAAAGGGCGT GCAAGGATTC AAGGGAGCAG AAGGTCTGCC TGGTATCCCT 
GGACCCAAAG GAGAATTCGG TTTCAAGGGT GAGAAGGGTT TGAGCGGAGC ACCCGGCAAC 
GACGGAACAC CCGGACGCGC TGGGCGGGAC GGATACCCCG GAATTCCCGG TCAATCCATC 
AAGGGCGAGC CAGGCTTCCA TGGAAGGGAC GGAGCAAAGG GCGACAAGGG ATCATTTGGC 
CGAAGCGGCG AGAAGGGAGA GCCCGGTAGC TGTGCGCTTG ACGAAATTAA GATGCCCGCC 
AAGGGTAACA AGGGTGAGCC CGGCCAAACC GGCATGCCAG GACCTCCGGG AGAAGACGGC 
AGCCCGGGAG AGAGGGGCTA TACCGGATTG AAGGGCAACA CTGGACCACA GGGACCTCCT 
GGCGTTGAAG GACCCCGCGG CTTGAATGGA CCTCGCGGTG AAAAGGGCAA CCAGGGCGCT 
GTCGGAGTAC CTGGTAATCC TGGCAAGGAC GGCCTTCGCG GCATTCCCGG ACGCAATGGA 
CAGCCTGGAC CGAGGGGAGA GCCTGGTATT TCGAGACCCG GCCCTATGGG CCCACCCGGT 
CTCAATGGTC TGCAAGGTGA GAAGGGCGAC CGTGGTCCAA CCGGACCCAT TGGTTTTCCC 
GGTGCCGATG GCAGTGTGGG ATATCCTGGA GATAGAGGCG ATGCCGGTCT GCCCGGAGTA 
TCTGGACGTC CCGGAATTGT TGGTGAGAAG GGAGACGTGG GCCCGATCGG ACCCGCTGGT 
GTTGCCGGAC CTCCTGGTGT TCCTGGTATT GATGGTGTGC GTGGACGTGA TGGCGCCAAG 
GGTGAGCCCG GCAGTCCCGG ATTGGTCGGC ATGCCCGGTA ACAAAGGTGA CCGTGGTGCT 
CCTGGAAATG ACGGACCCAA GGGCTTTGCT GGCGTTACTG GTGCTCCCGG AAAGCGCGGA 
CCTGCTGGTA TTCCCGGAGT TTCCGGTGCC AAGGGTGACA AGGGCGCTAC TGGCTTGACT 
GGCAACGATG GACCTGTGGG AGGCCGCGGT CCTCCAGGTG CTCCTGGACT GATGGGCATT 
AAGGGTGACC AAGGATTGGC AGGCGCCCCT GGACAACAAG GACTGGACGG TATGCCTGGC 
GAAAAGGGTA ACCAAGGATT CCCCGGTCTG GATGGACCTC CTGGTTTGCC TGGAGATGCC 
TCCGAGAAAG GACAAAAGGG TGAACCCGGT CCATCCGGAC TCCGCGGCGA TACAGGTCCG 
GCCGGAACGC CCGGTTGGCC AGGAGAGAAG GGTTTGCCCG GTCTGGCTGT TCACGGTCGT 
GCTGGTCCGC CAGGCGAGAA GGGTGACCAG GGACGCAGTG GAATCGATGG ACGAGATGGA 
ATTAACGGCG AGAAGGGTGA ACAAGGTCTG CAGGGCGTTT GGGGCCAGCC TGGCGAGAAG 
GGATCTGTCG GCGCACCTGG TATTCCTGGT GCTCCCGGAA TGGATGGTTT GCCCGGCGCT 
GCTGGTGCTC CTGGTGCTGT TGGCTATCCT GGTGATCGCG GTGACAAGGG AGAGCCTGGT 
CTATCTGGTC TGCCCGGACT CAAGGGTGAG ACTGGACCCG TTGGACTGCA GGGCTTCACC 
GGTGCTCCTG GCCCTAAGGG TGAGCGCGGT ATTCGTGGTC AGCCCGGTCT TCCGGCCACC 
GTTCCCGACA TCCGTGGTGA TAAGGGATCC CAGGGCGAGC GCGGCTACAC TGGCGAGAAG 
GGCGAGCAAG GCGAACGAGG CTTGACTGGT CCTGCTGGCG TCGCTGGAGC AAAGGGAGAT 
CGCGGATTGC AGGGACCACC AGGTGCAAGC GGATTGAACG GCATTCCCGG AGCCAAGGGA 
GACATTGGTC CAAGAGGCGA GATCGGTTAT CCAGGAGTTA CCATTAAGGG CGAGAAGGGT 
CTGCCCGGTC GCCCAGGCAG AAACGGACGT CAAGGTCTTA TTGGAGCACC CGGCTTAATT 
GGAGAACGTG GTCTGCCTGG CTTGGCCGGA GAGCCCGGCC TCGTGGGCCT GCCTGGACCC 
ATTGGACCAG CTGGCAGCAA GGGAGAGCGT GGTCTCGCCG GCAGTCCCGG ACAACCAGGA 
CAGGATGGCT TCCCCGGCGC ACCTGGATTG AAGGGAGATA CTGGACCGCA GGGCTTTAAG 
GGCGAACGTG GTCTGAATGG CTTCGAGGGA CAAAAGGGAG ACAAGGGTGA CCGAGGACTC 
CAAGGACCGT CTGGACTGCC CGGCTTGGTT GGACAGAAGG GAGATACCGG CTACCCTGGC 
TTAAATGGAA ACGATGGACC TGTCGGAGCT CCTGGCGAGC GCGGCTTCAC CGGGCCCAAG 
GGACGCGATG GACGCGACGG AACACCAGGT CTGCCTGGAC AGAAGGGTGA ACCAGGAATG 
CTGCCACCAC CAGGACCCAA GGGCGAACCT GGTCAGCCGG GACGCAATGG ACCTAAGGGA 
GAGCCCGGAC GTCCGGGAGA GCGTGGCTTG ATTGGCATCC AGGGTGAGCG TGGCGAAAAG 
GGTGAGCGCG GCCTGATCGG TGAGACTGGT AACGTGGGAC GACCCGGACC CAAGGGAGAT 
CGCGGAGAGC CAGGCGAGAG GGGATATGAG GGCGCCATTG GTTTGATCGG CCAGAAGGGT 
GAGCCCGGTG CTCCAGCCCC CGCTGCTCTG GACTATCTCA CCGGTATCCT GATTACGCGA 
CACAGTCAAT CGGAAACGGT GCCCGCTTGC TCGGCTGGAC ACACGGAACT GTGGACGGGT 
TACTCCCTGT TGTACGTCGA TGGCAATGAC TATGCCCACA ACCAGGACCT TGGATCCCCC 
GGATCCTGTG TGCCACGCTT CTCAACGCTG CCCGTACTGT CGTGTGGTCA GAATAACGTC 
TGCAACTACG CCTCCAGAAA TGACAAGACC TTCTGGCTGA CAACCAACGC CGCCATTCCG 
ATGATGCCCG TTGAAAACAT CGAGATCCGC CAGTACATCT CACGTTGCGT CGTTTGTGAG 
GCGCCGGCTA ATGTGATCGC CGTGCACAGT CAAACGATAG AGGTGCCCGA CTGTCCGAAT 
GGCTGGGAGG GTCTCTGGAT TGGCTACAGT TTCCTCATGC ACACTGCCGT GGGCAACGGT 
GGCGGTGGAC AGGCGCTGCA ATCGCCTGGC TCCTGTTTGG AGGACTTCCG TGCAACGCCC 
TTCATCGAGT GCAACGGCGC CAAGGGCACG TGCCACTTCT ATGAGACGAT GACAAGCTTC 
TGGATGTACA ACCTGGAGTC CTCACAGCCG TTCGAGAGGC CACAGCAGCA GACGATCAAG 
GCCGGCGAGC GGCAGTCGCA TGTGTCCAGG TGCCAGGTGT GCATGAAGAA CTCCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723046.1
CDS	103..5442
Translation	MLPFWKRLLYAAVIAGALVGADAQFWKTAGTAGSIQDSVKHYNRNEPKFPIDDSYDIVDSAGVARGDLPPKNCTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIGLIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQDLGSPGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPVENIEIRQYISRCVVCEAPANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECNGAKGTCHFYETMTSFWMYNLESSQPFERPQQQTIKAGERQSHVSRCQVCMKNSS

Target ORF information:

RefSeq Version	NM_164617.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster collagen type IV alpha 1, transcript variant C (Col4a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164617.2

ORF Insert Sequence:

ATGTTGCCCT TCTGGAAGCG GCTGCTATAC GCCGCTGTGA TCGCGGGAGC GTTAGTCGGT 
GCCGACGCTC AATTTTGGAA GACGGCTGGT ACGGCCGGTT CGATTCAGGA CTCCGTGAAA 
CACTACAATC GAAATGAACC CAAGTTCCCA ATCGACGACA GTTACGACAT TGTAGACTCT 
GCCGGCGTGG CGCGCGGCGA TCTGCCGCCC AAGAATTGTA CGGCCGGATA TGCGGGCTGT 
GTGCCCAAGT GCATAGCGGA GAAGGGCAAC CGTGGTCTGC CGGGCCCTCT TGGACCCACG 
GGATTGAAGG GCGAAATGGG TTTCCCTGGC ATGGAGGGAC CATCTGGTGA CAAGGGTCAG 
AAGGGTGATC CCGGCCCATA CGGACAGCGT GGTGATAAGG GTGAGCGCGG ATCGCCTGGT 
CTTCATGGTC AGGCTGGTGT ACCCGGAGTC CAGGGACCCG CCGGCAATCC CGGAGCCCCT 
GGTATCAACG GTAAAGACGG TTGCGACGGA CAGGATGGTA TTCCTGGCTT GGAGGGTCTA 
TCCGGAATGC CCGGACCTCG CGGATATGCC GGCCAGCTTG GCAGCAAGGG CGAGAAGGGT 
GAACCGGCAA AGGAGAACGG TGATTACGCG AAGGGCGAGA AGGGTGAGCC CGGTTGGAGG 
GGGACTGCCG GTTTGGCTGG ACCACAAGGA TTTCCTGGAG AAAAGGGCGA GCGCGGCGAC 
AGTGGACCTT ACGGAGCCAA AGGACCCCGG GGTGAGCACG GTCTGAAGGG AGAGAAGGGT 
GCCTCCTGCT ACGGACCCAT GAAGCCTGGT GCACCGGGTA TCAAGGGCGA GAAGGGTGAG 
CCCGCGTCCT CGTTTCCCGT CAAACCGACC CACACGGTGA TGGGACCTCG CGGCGATATG 
GGACAGAAGG GAGAGCCTGG CCTAGTTGGC CGCAAGGGTG AGCCCGGACC TGAAGGCGAC 
ACTGGACTCG ATGGACAGAA GGGCGAGAAG GGTCTGCCCG GCGGCCCAGG CGATCGCGGT 
CGCCAAGGTA ACTTTGGACC CCCAGGATCT ACAGGACAAA AGGGAGATCG TGGCGAGCCG 
GGCCTTAATG GTCTGCCCGG TAATCCCGGA CAGAAGGGTG AGCCAGGACG TGCTGGAGCG 
ACAGGTAAGC CTGGTCTGCT CGGTCCTCCG GGACCGCCAG GCGGTGGCCG TGGAACACCA 
GGACCCCCGG GACCCAAAGG ACCCCGCGGC TATGTTGGCG CACCTGGACC CCAGGGATTA 
AACGGAGTTG ATGGACTACC GGGTCCTCAG GGATACAATG GACAAAAGGG AGGTGCTGGT 
CTGCCCGGTC GCCCCGGCAA CGAGGGACCT CCTGGCAAAA AGGGAGAAAA GGGAACCGCA 
GGACTTAATG GACCAAAGGG ATCCATCGGA CCCATTGGAC ACCCAGGACC ACCGGGACCA 
GAGGGACAGA AGGGTGACGC CGGTTTGCCC GGTTATGGCA TTCAAGGATC TAAGGGAGAT 
GCTGGCATAC CTGGTTATCC CGGACTAAAG GGTAGCAAGG GAGAGCGCGG CTTCAAGGGC 
AATGCTGGTG CTCCCGGTGA CTCCAAGCTG GGTCGTCCTG GAACTCCCGG TGCCGCTGGT 
GCTCCTGGAC AAAAGGGAGA CGCTGGTCGT CCCGGCACTC CTGGCCAAAA GGGAGACATG 
GGTATCAAGG GTGACGTCGG CGGCAAATGC TCATCGTGCA GGGCCGGACC AAAGGGTGAT 
AAGGGAACGA GCGGACTGCC TGGAATTCCC GGAAAGGATG GCGCACGAGG ACCGCCTGGA 
GAGCGCGGAT ATCCCGGAGA GCGTGGACAC GATGGAATCA ACGGACAAAC TGGACCGCCT 
GGTGAGAAGG GAGAGGACGG TCGCACTGGT CTTCCCGGAG CAACTGGAGA GCCTGGCAAA 
CCTGCTCTGT GCGATTTGAG TTTGATTGAG CCATTGAAGG GTGACAAGGG TTACCCTGGT 
GCGCCAGGTG CAAAGGGCGT GCAAGGATTC AAGGGAGCAG AAGGTCTGCC TGGTATCCCT 
GGACCCAAAG GAGAATTCGG TTTCAAGGGT GAGAAGGGTT TGAGCGGAGC ACCCGGCAAC 
GACGGAACAC CCGGACGCGC TGGGCGGGAC GGATACCCCG GAATTCCCGG TCAATCCATC 
AAGGGCGAGC CAGGCTTCCA TGGAAGGGAC GGAGCAAAGG GCGACAAGGG ATCATTTGGC 
CGAAGCGGCG AGAAGGGAGA GCCCGGTAGC TGTGCGCTTG ACGAAATTAA GATGCCCGCC 
AAGGGTAACA AGGGTGAGCC CGGCCAAACC GGCATGCCAG GACCTCCGGG AGAAGACGGC 
AGCCCGGGAG AGAGGGGCTA TACCGGATTG AAGGGCAACA CTGGACCACA GGGACCTCCT 
GGCGTTGAAG GACCCCGCGG CTTGAATGGA CCTCGCGGTG AAAAGGGCAA CCAGGGCGCT 
GTCGGAGTAC CTGGTAATCC TGGCAAGGAC GGCCTTCGCG GCATTCCCGG ACGCAATGGA 
CAGCCTGGAC CGAGGGGAGA GCCTGGTATT TCGAGACCCG GCCCTATGGG CCCACCCGGT 
CTCAATGGTC TGCAAGGTGA GAAGGGCGAC CGTGGTCCAA CCGGACCCAT TGGTTTTCCC 
GGTGCCGATG GCAGTGTGGG ATATCCTGGA GATAGAGGCG ATGCCGGTCT GCCCGGAGTA 
TCTGGACGTC CCGGAATTGT TGGTGAGAAG GGAGACGTGG GCCCGATCGG ACCCGCTGGT 
GTTGCCGGAC CTCCTGGTGT TCCTGGTATT GATGGTGTGC GTGGACGTGA TGGCGCCAAG 
GGTGAGCCCG GCAGTCCCGG ATTGGTCGGC ATGCCCGGTA ACAAAGGTGA CCGTGGTGCT 
CCTGGAAATG ACGGACCCAA GGGCTTTGCT GGCGTTACTG GTGCTCCCGG AAAGCGCGGA 
CCTGCTGGTA TTCCCGGAGT TTCCGGTGCC AAGGGTGACA AGGGCGCTAC TGGCTTGACT 
GGCAACGATG GACCTGTGGG AGGCCGCGGT CCTCCAGGTG CTCCTGGACT GATGGGCATT 
AAGGGTGACC AAGGATTGGC AGGCGCCCCT GGACAACAAG GACTGGACGG TATGCCTGGC 
GAAAAGGGTA ACCAAGGATT CCCCGGTCTG GATGGACCTC CTGGTTTGCC TGGAGATGCC 
TCCGAGAAAG GACAAAAGGG TGAACCCGGT CCATCCGGAC TCCGCGGCGA TACAGGTCCG 
GCCGGAACGC CCGGTTGGCC AGGAGAGAAG GGTTTGCCCG GTCTGGCTGT TCACGGTCGT 
GCTGGTCCGC CAGGCGAGAA GGGTGACCAG GGACGCAGTG GAATCGATGG ACGAGATGGA 
ATTAACGGCG AGAAGGGTGA ACAAGGTCTG CAGGGCGTTT GGGGCCAGCC TGGCGAGAAG 
GGATCTGTCG GCGCACCTGG TATTCCTGGT GCTCCCGGAA TGGATGGTTT GCCCGGCGCT 
GCTGGTGCTC CTGGTGCTGT TGGCTATCCT GGTGATCGCG GTGACAAGGG AGAGCCTGGT 
CTATCTGGTC TGCCCGGACT CAAGGGTGAG ACTGGACCCG TTGGACTGCA GGGCTTCACC 
GGTGCTCCTG GCCCTAAGGG TGAGCGCGGT ATTCGTGGTC AGCCCGGTCT TCCGGCCACC 
GTTCCCGACA TCCGTGGTGA TAAGGGATCC CAGGGCGAGC GCGGCTACAC TGGCGAGAAG 
GGCGAGCAAG GCGAACGAGG CTTGACTGGT CCTGCTGGCG TCGCTGGAGC AAAGGGAGAT 
CGCGGATTGC AGGGACCACC AGGTGCAAGC GGATTGAACG GCATTCCCGG AGCCAAGGGA 
GACATTGGTC CAAGAGGCGA GATCGGTTAT CCAGGAGTTA CCATTAAGGG CGAGAAGGGT 
CTGCCCGGTC GCCCAGGCAG AAACGGACGT CAAGGTCTTA TTGGAGCACC CGGCTTAATT 
GGAGAACGTG GTCTGCCTGG CTTGGCCGGA GAGCCCGGCC TCGTGGGCCT GCCTGGACCC 
ATTGGACCAG CTGGCAGCAA GGGAGAGCGT GGTCTCGCCG GCAGTCCCGG ACAACCAGGA 
CAGGATGGCT TCCCCGGCGC ACCTGGATTG AAGGGAGATA CTGGACCGCA GGGCTTTAAG 
GGCGAACGTG GTCTGAATGG CTTCGAGGGA CAAAAGGGAG ACAAGGGTGA CCGAGGACTC 
CAAGGACCGT CTGGACTGCC CGGCTTGGTT GGACAGAAGG GAGATACCGG CTACCCTGGC 
TTAAATGGAA ACGATGGACC TGTCGGAGCT CCTGGCGAGC GCGGCTTCAC CGGGCCCAAG 
GGACGCGATG GACGCGACGG AACACCAGGT CTGCCTGGAC AGAAGGGTGA ACCAGGAATG 
CTGCCACCAC CAGGACCCAA GGGCGAACCT GGTCAGCCGG GACGCAATGG ACCTAAGGGA 
GAGCCCGGAC GTCCGGGAGA GCGTGGCTTG ATTGGCATCC AGGGTGAGCG TGGCGAAAAG 
GGTGAGCGCG GCCTGATCGG TGAGACTGGT AACGTGGGAC GACCCGGACC CAAGGGAGAT 
CGCGGAGAGC CAGGCGAGAG GGGATATGAG GGCGCCATTG GTTTGATCGG CCAGAAGGGT 
GAGCCCGGTG CTCCAGCCCC CGCTGCTCTG GACTATCTCA CCGGTATCCT GATTACGCGA 
CACAGTCAAT CGGAAACGGT GCCCGCTTGC TCGGCTGGAC ACACGGAACT GTGGACGGGT 
TACTCCCTGT TGTACGTCGA TGGCAATGAC TATGCCCACA ACCAGGACCT TGGATCCCCC 
GGATCCTGTG TGCCACGCTT CTCAACGCTG CCCGTACTGT CGTGTGGTCA GAATAACGTC 
TGCAACTACG CCTCCAGAAA TGACAAGACC TTCTGGCTGA CAACCAACGC CGCCATTCCG 
ATGATGCCCG TTGAAAACAT CGAGATCCGC CAGTACATCT CACGTTGCGT CGTTTGTGAG 
GCGCCGGCTA ATGTGATCGC CGTGCACAGT CAAACGATAG AGGTGCCCGA CTGTCCGAAT 
GGCTGGGAGG GTCTCTGGAT TGGCTACAGT TTCCTCATGC ACACTGCCGT GGGCAACGGT 
GGCGGTGGAC AGGCGCTGCA ATCGCCTGGC TCCTGTTTGG AGGACTTCCG TGCAACGCCC 
TTCATCGAGT GCAACGGCGC CAAGGGCACG TGCCACTTCT ATGAGACGAT GACAAGCTTC 
TGGATGTACA ACCTGGAGTC CTCACAGCCG TTCGAGAGGC CACAGCAGCA GACGATCAAG 
GCCGGCGAGC GGCAGTCGCA TGTGTCCAGG TGCCAGGTGT GCATGAAGAA CTCCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a1 ( NM_164616.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723045.1 Drosophila melanogaster collagen type IV alpha 1, transcript variant B (Col4a1), mRNA.

Price & Availability↑

CloneID	OFa16240
Clone ID Related Accession (Same CDS sequence)	NM_164615.2 , NM_164617.2 , NM_164616.2
Accession Version	NM_164616.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	collagen type IV alpha 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164616.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCCCT TCTGGAAGCG GCTGCTATAC GCCGCTGTGA TCGCGGGAGC GTTAGTCGGT 
GCCGACGCTC AATTTTGGAA GACGGCTGGT ACGGCCGGTT CGATTCAGGA CTCCGTGAAA 
CACTACAATC GAAATGAACC CAAGTTCCCA ATCGACGACA GTTACGACAT TGTAGACTCT 
GCCGGCGTGG CGCGCGGCGA TCTGCCGCCC AAGAATTGTA CGGCCGGATA TGCGGGCTGT 
GTGCCCAAGT GCATAGCGGA GAAGGGCAAC CGTGGTCTGC CGGGCCCTCT TGGACCCACG 
GGATTGAAGG GCGAAATGGG TTTCCCTGGC ATGGAGGGAC CATCTGGTGA CAAGGGTCAG 
AAGGGTGATC CCGGCCCATA CGGACAGCGT GGTGATAAGG GTGAGCGCGG ATCGCCTGGT 
CTTCATGGTC AGGCTGGTGT ACCCGGAGTC CAGGGACCCG CCGGCAATCC CGGAGCCCCT 
GGTATCAACG GTAAAGACGG TTGCGACGGA CAGGATGGTA TTCCTGGCTT GGAGGGTCTA 
TCCGGAATGC CCGGACCTCG CGGATATGCC GGCCAGCTTG GCAGCAAGGG CGAGAAGGGT 
GAACCGGCAA AGGAGAACGG TGATTACGCG AAGGGCGAGA AGGGTGAGCC CGGTTGGAGG 
GGGACTGCCG GTTTGGCTGG ACCACAAGGA TTTCCTGGAG AAAAGGGCGA GCGCGGCGAC 
AGTGGACCTT ACGGAGCCAA AGGACCCCGG GGTGAGCACG GTCTGAAGGG AGAGAAGGGT 
GCCTCCTGCT ACGGACCCAT GAAGCCTGGT GCACCGGGTA TCAAGGGCGA GAAGGGTGAG 
CCCGCGTCCT CGTTTCCCGT CAAACCGACC CACACGGTGA TGGGACCTCG CGGCGATATG 
GGACAGAAGG GAGAGCCTGG CCTAGTTGGC CGCAAGGGTG AGCCCGGACC TGAAGGCGAC 
ACTGGACTCG ATGGACAGAA GGGCGAGAAG GGTCTGCCCG GCGGCCCAGG CGATCGCGGT 
CGCCAAGGTA ACTTTGGACC CCCAGGATCT ACAGGACAAA AGGGAGATCG TGGCGAGCCG 
GGCCTTAATG GTCTGCCCGG TAATCCCGGA CAGAAGGGTG AGCCAGGACG TGCTGGAGCG 
ACAGGTAAGC CTGGTCTGCT CGGTCCTCCG GGACCGCCAG GCGGTGGCCG TGGAACACCA 
GGACCCCCGG GACCCAAAGG ACCCCGCGGC TATGTTGGCG CACCTGGACC CCAGGGATTA 
AACGGAGTTG ATGGACTACC GGGTCCTCAG GGATACAATG GACAAAAGGG AGGTGCTGGT 
CTGCCCGGTC GCCCCGGCAA CGAGGGACCT CCTGGCAAAA AGGGAGAAAA GGGAACCGCA 
GGACTTAATG GACCAAAGGG ATCCATCGGA CCCATTGGAC ACCCAGGACC ACCGGGACCA 
GAGGGACAGA AGGGTGACGC CGGTTTGCCC GGTTATGGCA TTCAAGGATC TAAGGGAGAT 
GCTGGCATAC CTGGTTATCC CGGACTAAAG GGTAGCAAGG GAGAGCGCGG CTTCAAGGGC 
AATGCTGGTG CTCCCGGTGA CTCCAAGCTG GGTCGTCCTG GAACTCCCGG TGCCGCTGGT 
GCTCCTGGAC AAAAGGGAGA CGCTGGTCGT CCCGGCACTC CTGGCCAAAA GGGAGACATG 
GGTATCAAGG GTGACGTCGG CGGCAAATGC TCATCGTGCA GGGCCGGACC AAAGGGTGAT 
AAGGGAACGA GCGGACTGCC TGGAATTCCC GGAAAGGATG GCGCACGAGG ACCGCCTGGA 
GAGCGCGGAT ATCCCGGAGA GCGTGGACAC GATGGAATCA ACGGACAAAC TGGACCGCCT 
GGTGAGAAGG GAGAGGACGG TCGCACTGGT CTTCCCGGAG CAACTGGAGA GCCTGGCAAA 
CCTGCTCTGT GCGATTTGAG TTTGATTGAG CCATTGAAGG GTGACAAGGG TTACCCTGGT 
GCGCCAGGTG CAAAGGGCGT GCAAGGATTC AAGGGAGCAG AAGGTCTGCC TGGTATCCCT 
GGACCCAAAG GAGAATTCGG TTTCAAGGGT GAGAAGGGTT TGAGCGGAGC ACCCGGCAAC 
GACGGAACAC CCGGACGCGC TGGGCGGGAC GGATACCCCG GAATTCCCGG TCAATCCATC 
AAGGGCGAGC CAGGCTTCCA TGGAAGGGAC GGAGCAAAGG GCGACAAGGG ATCATTTGGC 
CGAAGCGGCG AGAAGGGAGA GCCCGGTAGC TGTGCGCTTG ACGAAATTAA GATGCCCGCC 
AAGGGTAACA AGGGTGAGCC CGGCCAAACC GGCATGCCAG GACCTCCGGG AGAAGACGGC 
AGCCCGGGAG AGAGGGGCTA TACCGGATTG AAGGGCAACA CTGGACCACA GGGACCTCCT 
GGCGTTGAAG GACCCCGCGG CTTGAATGGA CCTCGCGGTG AAAAGGGCAA CCAGGGCGCT 
GTCGGAGTAC CTGGTAATCC TGGCAAGGAC GGCCTTCGCG GCATTCCCGG ACGCAATGGA 
CAGCCTGGAC CGAGGGGAGA GCCTGGTATT TCGAGACCCG GCCCTATGGG CCCACCCGGT 
CTCAATGGTC TGCAAGGTGA GAAGGGCGAC CGTGGTCCAA CCGGACCCAT TGGTTTTCCC 
GGTGCCGATG GCAGTGTGGG ATATCCTGGA GATAGAGGCG ATGCCGGTCT GCCCGGAGTA 
TCTGGACGTC CCGGAATTGT TGGTGAGAAG GGAGACGTGG GCCCGATCGG ACCCGCTGGT 
GTTGCCGGAC CTCCTGGTGT TCCTGGTATT GATGGTGTGC GTGGACGTGA TGGCGCCAAG 
GGTGAGCCCG GCAGTCCCGG ATTGGTCGGC ATGCCCGGTA ACAAAGGTGA CCGTGGTGCT 
CCTGGAAATG ACGGACCCAA GGGCTTTGCT GGCGTTACTG GTGCTCCCGG AAAGCGCGGA 
CCTGCTGGTA TTCCCGGAGT TTCCGGTGCC AAGGGTGACA AGGGCGCTAC TGGCTTGACT 
GGCAACGATG GACCTGTGGG AGGCCGCGGT CCTCCAGGTG CTCCTGGACT GATGGGCATT 
AAGGGTGACC AAGGATTGGC AGGCGCCCCT GGACAACAAG GACTGGACGG TATGCCTGGC 
GAAAAGGGTA ACCAAGGATT CCCCGGTCTG GATGGACCTC CTGGTTTGCC TGGAGATGCC 
TCCGAGAAAG GACAAAAGGG TGAACCCGGT CCATCCGGAC TCCGCGGCGA TACAGGTCCG 
GCCGGAACGC CCGGTTGGCC AGGAGAGAAG GGTTTGCCCG GTCTGGCTGT TCACGGTCGT 
GCTGGTCCGC CAGGCGAGAA GGGTGACCAG GGACGCAGTG GAATCGATGG ACGAGATGGA 
ATTAACGGCG AGAAGGGTGA ACAAGGTCTG CAGGGCGTTT GGGGCCAGCC TGGCGAGAAG 
GGATCTGTCG GCGCACCTGG TATTCCTGGT GCTCCCGGAA TGGATGGTTT GCCCGGCGCT 
GCTGGTGCTC CTGGTGCTGT TGGCTATCCT GGTGATCGCG GTGACAAGGG AGAGCCTGGT 
CTATCTGGTC TGCCCGGACT CAAGGGTGAG ACTGGACCCG TTGGACTGCA GGGCTTCACC 
GGTGCTCCTG GCCCTAAGGG TGAGCGCGGT ATTCGTGGTC AGCCCGGTCT TCCGGCCACC 
GTTCCCGACA TCCGTGGTGA TAAGGGATCC CAGGGCGAGC GCGGCTACAC TGGCGAGAAG 
GGCGAGCAAG GCGAACGAGG CTTGACTGGT CCTGCTGGCG TCGCTGGAGC AAAGGGAGAT 
CGCGGATTGC AGGGACCACC AGGTGCAAGC GGATTGAACG GCATTCCCGG AGCCAAGGGA 
GACATTGGTC CAAGAGGCGA GATCGGTTAT CCAGGAGTTA CCATTAAGGG CGAGAAGGGT 
CTGCCCGGTC GCCCAGGCAG AAACGGACGT CAAGGTCTTA TTGGAGCACC CGGCTTAATT 
GGAGAACGTG GTCTGCCTGG CTTGGCCGGA GAGCCCGGCC TCGTGGGCCT GCCTGGACCC 
ATTGGACCAG CTGGCAGCAA GGGAGAGCGT GGTCTCGCCG GCAGTCCCGG ACAACCAGGA 
CAGGATGGCT TCCCCGGCGC ACCTGGATTG AAGGGAGATA CTGGACCGCA GGGCTTTAAG 
GGCGAACGTG GTCTGAATGG CTTCGAGGGA CAAAAGGGAG ACAAGGGTGA CCGAGGACTC 
CAAGGACCGT CTGGACTGCC CGGCTTGGTT GGACAGAAGG GAGATACCGG CTACCCTGGC 
TTAAATGGAA ACGATGGACC TGTCGGAGCT CCTGGCGAGC GCGGCTTCAC CGGGCCCAAG 
GGACGCGATG GACGCGACGG AACACCAGGT CTGCCTGGAC AGAAGGGTGA ACCAGGAATG 
CTGCCACCAC CAGGACCCAA GGGCGAACCT GGTCAGCCGG GACGCAATGG ACCTAAGGGA 
GAGCCCGGAC GTCCGGGAGA GCGTGGCTTG ATTGGCATCC AGGGTGAGCG TGGCGAAAAG 
GGTGAGCGCG GCCTGATCGG TGAGACTGGT AACGTGGGAC GACCCGGACC CAAGGGAGAT 
CGCGGAGAGC CAGGCGAGAG GGGATATGAG GGCGCCATTG GTTTGATCGG CCAGAAGGGT 
GAGCCCGGTG CTCCAGCCCC CGCTGCTCTG GACTATCTCA CCGGTATCCT GATTACGCGA 
CACAGTCAAT CGGAAACGGT GCCCGCTTGC TCGGCTGGAC ACACGGAACT GTGGACGGGT 
TACTCCCTGT TGTACGTCGA TGGCAATGAC TATGCCCACA ACCAGGACCT TGGATCCCCC 
GGATCCTGTG TGCCACGCTT CTCAACGCTG CCCGTACTGT CGTGTGGTCA GAATAACGTC 
TGCAACTACG CCTCCAGAAA TGACAAGACC TTCTGGCTGA CAACCAACGC CGCCATTCCG 
ATGATGCCCG TTGAAAACAT CGAGATCCGC CAGTACATCT CACGTTGCGT CGTTTGTGAG 
GCGCCGGCTA ATGTGATCGC CGTGCACAGT CAAACGATAG AGGTGCCCGA CTGTCCGAAT 
GGCTGGGAGG GTCTCTGGAT TGGCTACAGT TTCCTCATGC ACACTGCCGT GGGCAACGGT 
GGCGGTGGAC AGGCGCTGCA ATCGCCTGGC TCCTGTTTGG AGGACTTCCG TGCAACGCCC 
TTCATCGAGT GCAACGGCGC CAAGGGCACG TGCCACTTCT ATGAGACGAT GACAAGCTTC 
TGGATGTACA ACCTGGAGTC CTCACAGCCG TTCGAGAGGC CACAGCAGCA GACGATCAAG 
GCCGGCGAGC GGCAGTCGCA TGTGTCCAGG TGCCAGGTGT GCATGAAGAA CTCCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723045.1
CDS	220..5559
Translation	MLPFWKRLLYAAVIAGALVGADAQFWKTAGTAGSIQDSVKHYNRNEPKFPIDDSYDIVDSAGVARGDLPPKNCTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIGLIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQDLGSPGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPVENIEIRQYISRCVVCEAPANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECNGAKGTCHFYETMTSFWMYNLESSQPFERPQQQTIKAGERQSHVSRCQVCMKNSS

Target ORF information:

RefSeq Version	NM_164616.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster collagen type IV alpha 1, transcript variant B (Col4a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164616.2

ORF Insert Sequence:

ATGTTGCCCT TCTGGAAGCG GCTGCTATAC GCCGCTGTGA TCGCGGGAGC GTTAGTCGGT 
GCCGACGCTC AATTTTGGAA GACGGCTGGT ACGGCCGGTT CGATTCAGGA CTCCGTGAAA 
CACTACAATC GAAATGAACC CAAGTTCCCA ATCGACGACA GTTACGACAT TGTAGACTCT 
GCCGGCGTGG CGCGCGGCGA TCTGCCGCCC AAGAATTGTA CGGCCGGATA TGCGGGCTGT 
GTGCCCAAGT GCATAGCGGA GAAGGGCAAC CGTGGTCTGC CGGGCCCTCT TGGACCCACG 
GGATTGAAGG GCGAAATGGG TTTCCCTGGC ATGGAGGGAC CATCTGGTGA CAAGGGTCAG 
AAGGGTGATC CCGGCCCATA CGGACAGCGT GGTGATAAGG GTGAGCGCGG ATCGCCTGGT 
CTTCATGGTC AGGCTGGTGT ACCCGGAGTC CAGGGACCCG CCGGCAATCC CGGAGCCCCT 
GGTATCAACG GTAAAGACGG TTGCGACGGA CAGGATGGTA TTCCTGGCTT GGAGGGTCTA 
TCCGGAATGC CCGGACCTCG CGGATATGCC GGCCAGCTTG GCAGCAAGGG CGAGAAGGGT 
GAACCGGCAA AGGAGAACGG TGATTACGCG AAGGGCGAGA AGGGTGAGCC CGGTTGGAGG 
GGGACTGCCG GTTTGGCTGG ACCACAAGGA TTTCCTGGAG AAAAGGGCGA GCGCGGCGAC 
AGTGGACCTT ACGGAGCCAA AGGACCCCGG GGTGAGCACG GTCTGAAGGG AGAGAAGGGT 
GCCTCCTGCT ACGGACCCAT GAAGCCTGGT GCACCGGGTA TCAAGGGCGA GAAGGGTGAG 
CCCGCGTCCT CGTTTCCCGT CAAACCGACC CACACGGTGA TGGGACCTCG CGGCGATATG 
GGACAGAAGG GAGAGCCTGG CCTAGTTGGC CGCAAGGGTG AGCCCGGACC TGAAGGCGAC 
ACTGGACTCG ATGGACAGAA GGGCGAGAAG GGTCTGCCCG GCGGCCCAGG CGATCGCGGT 
CGCCAAGGTA ACTTTGGACC CCCAGGATCT ACAGGACAAA AGGGAGATCG TGGCGAGCCG 
GGCCTTAATG GTCTGCCCGG TAATCCCGGA CAGAAGGGTG AGCCAGGACG TGCTGGAGCG 
ACAGGTAAGC CTGGTCTGCT CGGTCCTCCG GGACCGCCAG GCGGTGGCCG TGGAACACCA 
GGACCCCCGG GACCCAAAGG ACCCCGCGGC TATGTTGGCG CACCTGGACC CCAGGGATTA 
AACGGAGTTG ATGGACTACC GGGTCCTCAG GGATACAATG GACAAAAGGG AGGTGCTGGT 
CTGCCCGGTC GCCCCGGCAA CGAGGGACCT CCTGGCAAAA AGGGAGAAAA GGGAACCGCA 
GGACTTAATG GACCAAAGGG ATCCATCGGA CCCATTGGAC ACCCAGGACC ACCGGGACCA 
GAGGGACAGA AGGGTGACGC CGGTTTGCCC GGTTATGGCA TTCAAGGATC TAAGGGAGAT 
GCTGGCATAC CTGGTTATCC CGGACTAAAG GGTAGCAAGG GAGAGCGCGG CTTCAAGGGC 
AATGCTGGTG CTCCCGGTGA CTCCAAGCTG GGTCGTCCTG GAACTCCCGG TGCCGCTGGT 
GCTCCTGGAC AAAAGGGAGA CGCTGGTCGT CCCGGCACTC CTGGCCAAAA GGGAGACATG 
GGTATCAAGG GTGACGTCGG CGGCAAATGC TCATCGTGCA GGGCCGGACC AAAGGGTGAT 
AAGGGAACGA GCGGACTGCC TGGAATTCCC GGAAAGGATG GCGCACGAGG ACCGCCTGGA 
GAGCGCGGAT ATCCCGGAGA GCGTGGACAC GATGGAATCA ACGGACAAAC TGGACCGCCT 
GGTGAGAAGG GAGAGGACGG TCGCACTGGT CTTCCCGGAG CAACTGGAGA GCCTGGCAAA 
CCTGCTCTGT GCGATTTGAG TTTGATTGAG CCATTGAAGG GTGACAAGGG TTACCCTGGT 
GCGCCAGGTG CAAAGGGCGT GCAAGGATTC AAGGGAGCAG AAGGTCTGCC TGGTATCCCT 
GGACCCAAAG GAGAATTCGG TTTCAAGGGT GAGAAGGGTT TGAGCGGAGC ACCCGGCAAC 
GACGGAACAC CCGGACGCGC TGGGCGGGAC GGATACCCCG GAATTCCCGG TCAATCCATC 
AAGGGCGAGC CAGGCTTCCA TGGAAGGGAC GGAGCAAAGG GCGACAAGGG ATCATTTGGC 
CGAAGCGGCG AGAAGGGAGA GCCCGGTAGC TGTGCGCTTG ACGAAATTAA GATGCCCGCC 
AAGGGTAACA AGGGTGAGCC CGGCCAAACC GGCATGCCAG GACCTCCGGG AGAAGACGGC 
AGCCCGGGAG AGAGGGGCTA TACCGGATTG AAGGGCAACA CTGGACCACA GGGACCTCCT 
GGCGTTGAAG GACCCCGCGG CTTGAATGGA CCTCGCGGTG AAAAGGGCAA CCAGGGCGCT 
GTCGGAGTAC CTGGTAATCC TGGCAAGGAC GGCCTTCGCG GCATTCCCGG ACGCAATGGA 
CAGCCTGGAC CGAGGGGAGA GCCTGGTATT TCGAGACCCG GCCCTATGGG CCCACCCGGT 
CTCAATGGTC TGCAAGGTGA GAAGGGCGAC CGTGGTCCAA CCGGACCCAT TGGTTTTCCC 
GGTGCCGATG GCAGTGTGGG ATATCCTGGA GATAGAGGCG ATGCCGGTCT GCCCGGAGTA 
TCTGGACGTC CCGGAATTGT TGGTGAGAAG GGAGACGTGG GCCCGATCGG ACCCGCTGGT 
GTTGCCGGAC CTCCTGGTGT TCCTGGTATT GATGGTGTGC GTGGACGTGA TGGCGCCAAG 
GGTGAGCCCG GCAGTCCCGG ATTGGTCGGC ATGCCCGGTA ACAAAGGTGA CCGTGGTGCT 
CCTGGAAATG ACGGACCCAA GGGCTTTGCT GGCGTTACTG GTGCTCCCGG AAAGCGCGGA 
CCTGCTGGTA TTCCCGGAGT TTCCGGTGCC AAGGGTGACA AGGGCGCTAC TGGCTTGACT 
GGCAACGATG GACCTGTGGG AGGCCGCGGT CCTCCAGGTG CTCCTGGACT GATGGGCATT 
AAGGGTGACC AAGGATTGGC AGGCGCCCCT GGACAACAAG GACTGGACGG TATGCCTGGC 
GAAAAGGGTA ACCAAGGATT CCCCGGTCTG GATGGACCTC CTGGTTTGCC TGGAGATGCC 
TCCGAGAAAG GACAAAAGGG TGAACCCGGT CCATCCGGAC TCCGCGGCGA TACAGGTCCG 
GCCGGAACGC CCGGTTGGCC AGGAGAGAAG GGTTTGCCCG GTCTGGCTGT TCACGGTCGT 
GCTGGTCCGC CAGGCGAGAA GGGTGACCAG GGACGCAGTG GAATCGATGG ACGAGATGGA 
ATTAACGGCG AGAAGGGTGA ACAAGGTCTG CAGGGCGTTT GGGGCCAGCC TGGCGAGAAG 
GGATCTGTCG GCGCACCTGG TATTCCTGGT GCTCCCGGAA TGGATGGTTT GCCCGGCGCT 
GCTGGTGCTC CTGGTGCTGT TGGCTATCCT GGTGATCGCG GTGACAAGGG AGAGCCTGGT 
CTATCTGGTC TGCCCGGACT CAAGGGTGAG ACTGGACCCG TTGGACTGCA GGGCTTCACC 
GGTGCTCCTG GCCCTAAGGG TGAGCGCGGT ATTCGTGGTC AGCCCGGTCT TCCGGCCACC 
GTTCCCGACA TCCGTGGTGA TAAGGGATCC CAGGGCGAGC GCGGCTACAC TGGCGAGAAG 
GGCGAGCAAG GCGAACGAGG CTTGACTGGT CCTGCTGGCG TCGCTGGAGC AAAGGGAGAT 
CGCGGATTGC AGGGACCACC AGGTGCAAGC GGATTGAACG GCATTCCCGG AGCCAAGGGA 
GACATTGGTC CAAGAGGCGA GATCGGTTAT CCAGGAGTTA CCATTAAGGG CGAGAAGGGT 
CTGCCCGGTC GCCCAGGCAG AAACGGACGT CAAGGTCTTA TTGGAGCACC CGGCTTAATT 
GGAGAACGTG GTCTGCCTGG CTTGGCCGGA GAGCCCGGCC TCGTGGGCCT GCCTGGACCC 
ATTGGACCAG CTGGCAGCAA GGGAGAGCGT GGTCTCGCCG GCAGTCCCGG ACAACCAGGA 
CAGGATGGCT TCCCCGGCGC ACCTGGATTG AAGGGAGATA CTGGACCGCA GGGCTTTAAG 
GGCGAACGTG GTCTGAATGG CTTCGAGGGA CAAAAGGGAG ACAAGGGTGA CCGAGGACTC 
CAAGGACCGT CTGGACTGCC CGGCTTGGTT GGACAGAAGG GAGATACCGG CTACCCTGGC 
TTAAATGGAA ACGATGGACC TGTCGGAGCT CCTGGCGAGC GCGGCTTCAC CGGGCCCAAG 
GGACGCGATG GACGCGACGG AACACCAGGT CTGCCTGGAC AGAAGGGTGA ACCAGGAATG 
CTGCCACCAC CAGGACCCAA GGGCGAACCT GGTCAGCCGG GACGCAATGG ACCTAAGGGA 
GAGCCCGGAC GTCCGGGAGA GCGTGGCTTG ATTGGCATCC AGGGTGAGCG TGGCGAAAAG 
GGTGAGCGCG GCCTGATCGG TGAGACTGGT AACGTGGGAC GACCCGGACC CAAGGGAGAT 
CGCGGAGAGC CAGGCGAGAG GGGATATGAG GGCGCCATTG GTTTGATCGG CCAGAAGGGT 
GAGCCCGGTG CTCCAGCCCC CGCTGCTCTG GACTATCTCA CCGGTATCCT GATTACGCGA 
CACAGTCAAT CGGAAACGGT GCCCGCTTGC TCGGCTGGAC ACACGGAACT GTGGACGGGT 
TACTCCCTGT TGTACGTCGA TGGCAATGAC TATGCCCACA ACCAGGACCT TGGATCCCCC 
GGATCCTGTG TGCCACGCTT CTCAACGCTG CCCGTACTGT CGTGTGGTCA GAATAACGTC 
TGCAACTACG CCTCCAGAAA TGACAAGACC TTCTGGCTGA CAACCAACGC CGCCATTCCG 
ATGATGCCCG TTGAAAACAT CGAGATCCGC CAGTACATCT CACGTTGCGT CGTTTGTGAG 
GCGCCGGCTA ATGTGATCGC CGTGCACAGT CAAACGATAG AGGTGCCCGA CTGTCCGAAT 
GGCTGGGAGG GTCTCTGGAT TGGCTACAGT TTCCTCATGC ACACTGCCGT GGGCAACGGT 
GGCGGTGGAC AGGCGCTGCA ATCGCCTGGC TCCTGTTTGG AGGACTTCCG TGCAACGCCC 
TTCATCGAGT GCAACGGCGC CAAGGGCACG TGCCACTTCT ATGAGACGAT GACAAGCTTC 
TGGATGTACA ACCTGGAGTC CTCACAGCCG TTCGAGAGGC CACAGCAGCA GACGATCAAG 
GCCGGCGAGC GGCAGTCGCA TGTGTCCAGG TGCCAGGTGT GCATGAAGAA CTCCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Col4a1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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