COL4A2 cDNA ORF clone, Gallus gallus(chicken)

The following COL4A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa142266 XM_015277857.2
Latest version!
Gallus gallus collagen type IV alpha 2 chain (COL4A2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa25906 NM_001162390.1
Latest version!
Gallus gallus collagen type IV alpha 2 chain (COL4A2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa25906 XM_015277855.2
Latest version!
Gallus gallus collagen type IV alpha 2 chain (COL4A2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa25906 XM_015277856.2
Latest version!
Gallus gallus collagen type IV alpha 2 chain (COL4A2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa30679 XM_015277855.1
Latest version!
Gallus gallus collagen, type IV, alpha 2 (COL4A2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa30679 XM_015277856.1
Latest version!
Gallus gallus collagen, type IV, alpha 2 (COL4A2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa30680 XM_015277857.1
Latest version!
Gallus gallus collagen, type IV, alpha 2 (COL4A2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa30681 XM_015277858.2
Latest version!
Gallus gallus collagen type IV alpha 2 chain (COL4A2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OGa30681 XM_015277858.1 Gallus gallus collagen, type IV, alpha 2 (COL4A2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa142266
    Clone ID Related Accession (Same CDS sequence) XM_015277857.2
    Accession Version XM_015277857.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-2(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGATCC CAGGACTGCA AGGCCCTAAA GGTCACAAGG GTGAAAGAGG ACATCCAGGA 
    ATAAGTGGAC CCAAAGGAGA AACGGGGCAA AGAGGAGTCA CTGGTTTCCC AGGGGCAGAT
    GGCGTTCCTG GTCATCCAGG GCAGCCAGGA TCAAGGGGGA AGCCAGGTCA TGATGGCTGT
    AATGGGACAG TAGGTGACCC AGGTGACCCA GGAACACCAG GACACTCTGG TTTCCCTGGT
    ACCATTGGAG TTCAAGGACC AAAGGGCCAG AAGGGGGAGC CCTATGTGCT ACCGCCAGAC
    ATCGCCAGCC GACATCGGGG GGATCCTGGG GACCCAGGAT TCACTGGTTT TCCGGGAGCT
    CCGGGTACAT TGGGTATTCA AGGACCTATT GGTCCAAGAG GAGTGCCAGG AAGACCTGGA
    CCACCGGGGT CCCCAGGACC GCAAGGACCA CAAGGCAACA GAGGTCTTGG CTTCTATGGC
    GAAAAAGGTG AACAGGGCTC CCCAGGTCCT CCAGGTCCAC CTGGACTTCC CACAAGAGAA
    CTGATTGGTG TCCCCACTGA CAAACACAAA GGTGAAAGAG GAGAGCCTGG ACAAAAAGGG
    GAAGCTGGAT TTCCAGGTGT ACTTCTCTTC GCTCCTGAAA AAGGTGAAGA AGGGGTAATG
    GGCTTCCCAG GACAAAGGGG ACTGCCTGGT AACGATGGTT TTCCAGGACT CAGTGGAGAA
    AGAGGTTTTC CAGGATTTGA TGGTCAACCA GGTCAATATG GTCCTAGAGG AGGCAAGGGG
    GAGCAGGGTG AGATGGGTCC TCCAGGACCA CCTGCCTATG TTCCTTACCG CATTCCAAGA
    AAAGGTGTAA GAGGTGATCC AGGCTCTCCA GGTGCTTCTG GACTACGGGG AGAACAAGGA
    GAGCAGGGTG ATCGTGGGCT GCCTGGTATT CCAGGATTCT CTGATGGAGA TGCAGATAAG
    CCAGGCTTAC CTGGAGAAAT AGGACCAAAA GGTGAAAAAG GTGAAGAAGG ATCTCCTGCT
    TATCAAGCAG GCCCGCCTGG GTTTCCAGGT AAACACGGTG AACCAGGAAT CCGTGGTCCA
    CCTGGTCCCC CTGGAACTCC AGGTTCTCTC TTCGGATTAA AAGGACAGGA AGGGACACCA
    GGTAGACCTG GTGTACAAGG TTTTCCAGGG CCAAGAGGAC AAAGAGGACC TAAAGGTGAA
    GAAGGTGACT GCAGTAAATG CCTTCTGAGT GATGAACTCA GAAGGGGCTC TACTGGCCCC
    CGTGGTCCTC CTGGTTTTCC AGGAACCCCT GGCCAGCCTG GGAGAAAAGG AGAACCTGGT
    GATCAAGGTC CACATGGTAT TCCTGGCTAT GCTGGAGCAA AAGGACAATC GGGCCAAGAA
    GGATTACCTG GACCAAAAGG AGAAAAGGGA GATTCTATAT ACATAACTAC TAAAGGCACC
    AAAGGAATCC GAGGAGATCC TGGACTTCCT GGTATCAGAG GGGAAGATGG TTTCCCAGGC
    AGAGATGGAT TAGATGGTTT ACCAGGACTG CCTGGCCTTC CGGGTGATGG TATCAGAGGT
    CTCCCCGGGG ATCCTGGTTA TCCAGGAGAA TTAGGCCCAA AGGGCTTTCC AGGAGAAATA
    GGCCTACCAG GTGAAGGATA TCCTGGCCCA AAAGGATACC GTGGTCTTCC AGGTGACCGT
    GGAACAGATG GACACCCTGG GCCTCCAGGT CTGCCTGGTC CACCAGGAGA GCCTGGCCAA
    TTAGACTGCG GGCAGGTGAT TGAAGATTTT TCTAGAGGAG AAGCAACTGA TCCAATATGG
    TCAGGTGGAG GCTGCGTGAG GCCACCAAAG GGATCTCAAG GCAACCCAGG GCTGCCAGGA
    GCCACAGGTA CTAAAGGTGC AAGAGGATTC CCGGGTGATC CAGGCCCAGT GGGATTCCCA
    GGGCTAAATG GTACACGAGG TGATCCAGGT CGGGAAGGAT ATCCAGGTCC TCCAGGCTTT
    ATTGGGCCCC GGGGAGACAG AGGCCCAAAT GGCCTACCTG GACTACAGGG ACATCCAGGT
    CTTATGGGAA AGTCTGGTGC TCCAGGACTT GCTGGACAGA AGGGTGCTCC TGGTGACGTT
    CTGGGAGCAG CAGCAGGTCC TCGAGGGGAT GATGGGCTGC CTGGCTTCCC TGGCTTGAAA
    GGTGCACCAG GAGATCAAGG AATACCAGGA ATTAGAGGAG CGGACGGTAA CCCAGGATTG
    CCAGGCCCCA AAGGTGACCC AGGGCTGCAA GGTCTCCCTG GTTTGATGGG TTTGCCAGGA
    ACACCAGGAA CTCATGGATT CCCAGGGCCA CCAGGAAACC GCGGCCCTGA CGGTGGACCC
    GGCTCTCAAG GACCCCTTGG TCCACCAGGT GCAAGGGGAG AAGATGGCGA GCAAGGCTTT
    CCTGGTCCCG TGGGCATGAA AGGTCTGTCT GGTGACAAAG GTGAAACAGG TTTCCCAGGA
    TTACAAGGTA TCCCTGGTGT GACCGGTCCC CCTGGCATTA GTGGGATGGA TGGCTTCCCA
    GGAGACAAAG GCTCAAGAGG TTCACCTGGC ATTGATGGTT TCAAAGGCAT GCCAGGCCTG
    AAAGGAAGAC CAGGAATCAA AGGAATCAAA GGAGAATTTG GCTTACTTGG GACTCGGGGT
    GATAAAGGCG CACAGGGTGC ACGAGGCTTT AAAGGTGACC GTGGAGAGCA AGGTCCCCCA
    GGAGAGCCCC CGAAGCTGAA GCCTAGCATG ATGATGGAAG TTAAAGGTGA AAAAGGAGAT
    GCTGGAGAAA CTGGCACCAA GGGATTCTTT GGCATAAAAG GTAGCAAGGG AATGCCAGGC
    CTCCCCGGTA AGACAGGAAT TCCTGGGTCC CCAGGGCACC CCAGTTATGT GCCTGGTGTG
    AAGGGGGACA TCGGTGCAAA AGGGCTGACG GGTCTGAAAG GGTATCCTGG TCCGACTGGC
    TCTCCAGGCA TCAGAGGCTT CCCTGGCAGC ACAGGAGGCA GGGGAGATAA GGGTGCCCCA
    GGAATTTCGG GCCATTTTGG TACTCCTGGC TCCCATGGTG AAATTGGTGA GCCGGGCGAT
    ACTATAAACT TACCAGGAAT GCCAGGCCTC AAAGGGGAAG TTGGTGTGCC GGGTCTTACA
    GGCTTAAGAG GAGGCCCTGG ACAAAAAGGT GAAGGAGGTG ATCCTGGTTT ACCTGGCATT
    GAAGGCCTCA AGGGCATACA GGGTGTACCT GGCTCCTTGG GACAGAAAGG TTTGCCTGGA
    CTGGTCGGCC CTCCAGGGCA GCAAGGAAGC CCCGGGACGC CTGGATTCCA AGGAGAAAAA
    GGAGCTCCCG GCTGGCCTGG CTTACCAGGA CAAGCAGGCT TGCCTGGCTT AAGAGGAATC
    AGTGGACTGC ATGGTTTACC GGGCACCAAG GGCTTACCAG GATCACCAGG TCCAGACGGC
    TATGGCTCTG CGGGCTTCCC AGGCCCTGTG GGTGACAAAG GAGAAGCAGG AGAGCCCAGC
    AGAGTGGAGG GCAGCCAAGG CCCGCCGGGT CAGAAGGGAG ACAGAGGTGT TCCAGGAGTC
    CAAGGACCCT TTGGCATTCC TGGGCAGGAT GGACTTCCAG GGCCTCCTGG GATTTCCAAT
    ATATCAGGAT ATCCAGGCGA TACAGGTTCC CCAGGTCTAG ATGGAGTGCC AGGCTATCCA
    GGACTACATG GGCAGCCTGG AATACCAGCT CCACCGGGAA GCAAGGGAGA AAGTGGACGA
    GCAGGTGTGA GCGGACAAGC TGGCCCCAAA GGGACAAGAG GTGATCCTGG ATTACCAGGG
    AGACCTGGGA TTCCTGGTTA TCCTGGACCA AAAGGTCGTA AGGGTGAACA AGGTGTCATT
    GGCTTTATTG GCACAGTAGG ATTTCCAGGT GATTTGGGAC CCATCGGACC CAAGGGGGAT
    AGAGGGCTTA CTGGCTTTCA GGGCCCTCCA GGCTCTCCTG GGTTGCCACC AATTCCTCCA
    AGGCTGGTTG CTGAGCAAGG TTCACCAGGT CCCCGTGGAA ATGCAGGGCC TCGGGGAAGT
    CCGGGTGATG CAGGACCTCA AGGTCCACCA GGAGAACCAG GTCTCAGAGG CTTACCTGGA
    GAACCAGGAC TCCAGGGCAG AGGTGGCATT CCAGCTCCTC CTGGATCCAG AGGTGAACAA
    GGAGCAATGG GGTTTCAAGG TCCAGTGGGA TTTGAAGGCC AGCCAGGTCG TCCTGGTAGC
    CCTGGGCTGC CAGGCATGCC GGGTCGCAGC GTTAGCATCG GCTACCTGCT GGTGAAGCAC
    AGCCAGTCCG ACCAAGAGCC AATGTGCCCG ATTGGCATGA ACAAACTCTG GAGTGGCTAC
    AGCCTCCTGT ACTTTGAGGG ACAAGAGAAA GCACACAACC AGGACCTAGG GCTGGCTGGC
    TCATGCTTGG CTCGTTTCAG TACAATGCCA TTCCTCTACT GTAACCCTGG GGATATTTGT
    TATTATGCAA ATCGAAATGA CAAATCCTAC TGGCTCTCAA CTACAGCACC TCTTCCAATG
    ATGCCTGTGG CAGAAGAAGA AATTAGGCCA TATATCAGCC GCTGCTCTGT GTGTGAGGCC
    CCAGCTGTGG CAATTGCTGT CCATAGCCAA GAAGCTTCTA TTCCTCGCTG CCCTGAAGGC
    TGGCGCAGTT TATGGATTGG ATATTCCTTC CTTATGCACA CTGCGGCTGG TGATGAAGGC
    GGAGGACAGT CGCTGGTCTC TCCTGGGAGC TGCCTGGAAG ATTTCAGGGC TACTCCTTTC
    ATCGAGTGCA ATGGGGCCCG TGGAACCTGT CATTACTTTG CCAATAAGTA CAGCTTCTGG
    CTCACTACGA TTGACCAGCC CTTCCAAAGC AAGCCATCGG CAGACACACT CAAGGCAGGA
    CTCATCCGAA GCCACATCAG CAGATGTCAA GTCTGTATGA AAAATTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133343.2
    CDS152..5062
    Translation

    Target ORF information:

    RefSeq Version XM_015277857.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 2 chain (COL4A2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277857.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGATCC CAGGACTGCA AGGCCCTAAA GGTCACAAGG GTGAAAGAGG ACATCCAGGA 
    ATAAGTGGAC CCAAAGGAGA AACGGGGCAA AGAGGAGTCA CTGGTTTCCC AGGGGCAGAT
    GGCGTTCCTG GTCATCCAGG GCAGCCAGGA TCAAGGGGGA AGCCAGGTCA TGATGGCTGT
    AATGGGACAG TAGGTGACCC AGGTGACCCA GGAACACCAG GACACTCTGG TTTCCCTGGT
    ACCATTGGAG TTCAAGGACC AAAGGGCCAG AAGGGGGAGC CCTATGTGCT ACCGCCAGAC
    ATCGCCAGCC GACATCGGGG GGATCCTGGG GACCCAGGAT TCACTGGTTT TCCGGGAGCT
    CCGGGTACAT TGGGTATTCA AGGACCTATT GGTCCAAGAG GAGTGCCAGG AAGACCTGGA
    CCACCGGGGT CCCCAGGACC GCAAGGACCA CAAGGCAACA GAGGTCTTGG CTTCTATGGC
    GAAAAAGGTG AACAGGGCTC CCCAGGTCCT CCAGGTCCAC CTGGACTTCC CACAAGAGAA
    CTGATTGGTG TCCCCACTGA CAAACACAAA GGTGAAAGAG GAGAGCCTGG ACAAAAAGGG
    GAAGCTGGAT TTCCAGGTGT ACTTCTCTTC GCTCCTGAAA AAGGTGAAGA AGGGGTAATG
    GGCTTCCCAG GACAAAGGGG ACTGCCTGGT AACGATGGTT TTCCAGGACT CAGTGGAGAA
    AGAGGTTTTC CAGGATTTGA TGGTCAACCA GGTCAATATG GTCCTAGAGG AGGCAAGGGG
    GAGCAGGGTG AGATGGGTCC TCCAGGACCA CCTGCCTATG TTCCTTACCG CATTCCAAGA
    AAAGGTGTAA GAGGTGATCC AGGCTCTCCA GGTGCTTCTG GACTACGGGG AGAACAAGGA
    GAGCAGGGTG ATCGTGGGCT GCCTGGTATT CCAGGATTCT CTGATGGAGA TGCAGATAAG
    CCAGGCTTAC CTGGAGAAAT AGGACCAAAA GGTGAAAAAG GTGAAGAAGG ATCTCCTGCT
    TATCAAGCAG GCCCGCCTGG GTTTCCAGGT AAACACGGTG AACCAGGAAT CCGTGGTCCA
    CCTGGTCCCC CTGGAACTCC AGGTTCTCTC TTCGGATTAA AAGGACAGGA AGGGACACCA
    GGTAGACCTG GTGTACAAGG TTTTCCAGGG CCAAGAGGAC AAAGAGGACC TAAAGGTGAA
    GAAGGTGACT GCAGTAAATG CCTTCTGAGT GATGAACTCA GAAGGGGCTC TACTGGCCCC
    CGTGGTCCTC CTGGTTTTCC AGGAACCCCT GGCCAGCCTG GGAGAAAAGG AGAACCTGGT
    GATCAAGGTC CACATGGTAT TCCTGGCTAT GCTGGAGCAA AAGGACAATC GGGCCAAGAA
    GGATTACCTG GACCAAAAGG AGAAAAGGGA GATTCTATAT ACATAACTAC TAAAGGCACC
    AAAGGAATCC GAGGAGATCC TGGACTTCCT GGTATCAGAG GGGAAGATGG TTTCCCAGGC
    AGAGATGGAT TAGATGGTTT ACCAGGACTG CCTGGCCTTC CGGGTGATGG TATCAGAGGT
    CTCCCCGGGG ATCCTGGTTA TCCAGGAGAA TTAGGCCCAA AGGGCTTTCC AGGAGAAATA
    GGCCTACCAG GTGAAGGATA TCCTGGCCCA AAAGGATACC GTGGTCTTCC AGGTGACCGT
    GGAACAGATG GACACCCTGG GCCTCCAGGT CTGCCTGGTC CACCAGGAGA GCCTGGCCAA
    TTAGACTGCG GGCAGGTGAT TGAAGATTTT TCTAGAGGAG AAGCAACTGA TCCAATATGG
    TCAGGTGGAG GCTGCGTGAG GCCACCAAAG GGATCTCAAG GCAACCCAGG GCTGCCAGGA
    GCCACAGGTA CTAAAGGTGC AAGAGGATTC CCGGGTGATC CAGGCCCAGT GGGATTCCCA
    GGGCTAAATG GTACACGAGG TGATCCAGGT CGGGAAGGAT ATCCAGGTCC TCCAGGCTTT
    ATTGGGCCCC GGGGAGACAG AGGCCCAAAT GGCCTACCTG GACTACAGGG ACATCCAGGT
    CTTATGGGAA AGTCTGGTGC TCCAGGACTT GCTGGACAGA AGGGTGCTCC TGGTGACGTT
    CTGGGAGCAG CAGCAGGTCC TCGAGGGGAT GATGGGCTGC CTGGCTTCCC TGGCTTGAAA
    GGTGCACCAG GAGATCAAGG AATACCAGGA ATTAGAGGAG CGGACGGTAA CCCAGGATTG
    CCAGGCCCCA AAGGTGACCC AGGGCTGCAA GGTCTCCCTG GTTTGATGGG TTTGCCAGGA
    ACACCAGGAA CTCATGGATT CCCAGGGCCA CCAGGAAACC GCGGCCCTGA CGGTGGACCC
    GGCTCTCAAG GACCCCTTGG TCCACCAGGT GCAAGGGGAG AAGATGGCGA GCAAGGCTTT
    CCTGGTCCCG TGGGCATGAA AGGTCTGTCT GGTGACAAAG GTGAAACAGG TTTCCCAGGA
    TTACAAGGTA TCCCTGGTGT GACCGGTCCC CCTGGCATTA GTGGGATGGA TGGCTTCCCA
    GGAGACAAAG GCTCAAGAGG TTCACCTGGC ATTGATGGTT TCAAAGGCAT GCCAGGCCTG
    AAAGGAAGAC CAGGAATCAA AGGAATCAAA GGAGAATTTG GCTTACTTGG GACTCGGGGT
    GATAAAGGCG CACAGGGTGC ACGAGGCTTT AAAGGTGACC GTGGAGAGCA AGGTCCCCCA
    GGAGAGCCCC CGAAGCTGAA GCCTAGCATG ATGATGGAAG TTAAAGGTGA AAAAGGAGAT
    GCTGGAGAAA CTGGCACCAA GGGATTCTTT GGCATAAAAG GTAGCAAGGG AATGCCAGGC
    CTCCCCGGTA AGACAGGAAT TCCTGGGTCC CCAGGGCACC CCAGTTATGT GCCTGGTGTG
    AAGGGGGACA TCGGTGCAAA AGGGCTGACG GGTCTGAAAG GGTATCCTGG TCCGACTGGC
    TCTCCAGGCA TCAGAGGCTT CCCTGGCAGC ACAGGAGGCA GGGGAGATAA GGGTGCCCCA
    GGAATTTCGG GCCATTTTGG TACTCCTGGC TCCCATGGTG AAATTGGTGA GCCGGGCGAT
    ACTATAAACT TACCAGGAAT GCCAGGCCTC AAAGGGGAAG TTGGTGTGCC GGGTCTTACA
    GGCTTAAGAG GAGGCCCTGG ACAAAAAGGT GAAGGAGGTG ATCCTGGTTT ACCTGGCATT
    GAAGGCCTCA AGGGCATACA GGGTGTACCT GGCTCCTTGG GACAGAAAGG TTTGCCTGGA
    CTGGTCGGCC CTCCAGGGCA GCAAGGAAGC CCCGGGACGC CTGGATTCCA AGGAGAAAAA
    GGAGCTCCCG GCTGGCCTGG CTTACCAGGA CAAGCAGGCT TGCCTGGCTT AAGAGGAATC
    AGTGGACTGC ATGGTTTACC GGGCACCAAG GGCTTACCAG GATCACCAGG TCCAGACGGC
    TATGGCTCTG CGGGCTTCCC AGGCCCTGTG GGTGACAAAG GAGAAGCAGG AGAGCCCAGC
    AGAGTGGAGG GCAGCCAAGG CCCGCCGGGT CAGAAGGGAG ACAGAGGTGT TCCAGGAGTC
    CAAGGACCCT TTGGCATTCC TGGGCAGGAT GGACTTCCAG GGCCTCCTGG GATTTCCAAT
    ATATCAGGAT ATCCAGGCGA TACAGGTTCC CCAGGTCTAG ATGGAGTGCC AGGCTATCCA
    GGACTACATG GGCAGCCTGG AATACCAGCT CCACCGGGAA GCAAGGGAGA AAGTGGACGA
    GCAGGTGTGA GCGGACAAGC TGGCCCCAAA GGGACAAGAG GTGATCCTGG ATTACCAGGG
    AGACCTGGGA TTCCTGGTTA TCCTGGACCA AAAGGTCGTA AGGGTGAACA AGGTGTCATT
    GGCTTTATTG GCACAGTAGG ATTTCCAGGT GATTTGGGAC CCATCGGACC CAAGGGGGAT
    AGAGGGCTTA CTGGCTTTCA GGGCCCTCCA GGCTCTCCTG GGTTGCCACC AATTCCTCCA
    AGGCTGGTTG CTGAGCAAGG TTCACCAGGT CCCCGTGGAA ATGCAGGGCC TCGGGGAAGT
    CCGGGTGATG CAGGACCTCA AGGTCCACCA GGAGAACCAG GTCTCAGAGG CTTACCTGGA
    GAACCAGGAC TCCAGGGCAG AGGTGGCATT CCAGCTCCTC CTGGATCCAG AGGTGAACAA
    GGAGCAATGG GGTTTCAAGG TCCAGTGGGA TTTGAAGGCC AGCCAGGTCG TCCTGGTAGC
    CCTGGGCTGC CAGGCATGCC GGGTCGCAGC GTTAGCATCG GCTACCTGCT GGTGAAGCAC
    AGCCAGTCCG ACCAAGAGCC AATGTGCCCG ATTGGCATGA ACAAACTCTG GAGTGGCTAC
    AGCCTCCTGT ACTTTGAGGG ACAAGAGAAA GCACACAACC AGGACCTAGG GCTGGCTGGC
    TCATGCTTGG CTCGTTTCAG TACAATGCCA TTCCTCTACT GTAACCCTGG GGATATTTGT
    TATTATGCAA ATCGAAATGA CAAATCCTAC TGGCTCTCAA CTACAGCACC TCTTCCAATG
    ATGCCTGTGG CAGAAGAAGA AATTAGGCCA TATATCAGCC GCTGCTCTGT GTGTGAGGCC
    CCAGCTGTGG CAATTGCTGT CCATAGCCAA GAAGCTTCTA TTCCTCGCTG CCCTGAAGGC
    TGGCGCAGTT TATGGATTGG ATATTCCTTC CTTATGCACA CTGCGGCTGG TGATGAAGGC
    GGAGGACAGT CGCTGGTCTC TCCTGGGAGC TGCCTGGAAG ATTTCAGGGC TACTCCTTTC
    ATCGAGTGCA ATGGGGCCCG TGGAACCTGT CATTACTTTG CCAATAAGTA CAGCTTCTGG
    CTCACTACGA TTGACCAGCC CTTCCAAAGC AAGCCATCGG CAGACACACT CAAGGCAGGA
    CTCATCCGAA GCCACATCAG CAGATGTCAA GTCTGTATGA AAAATTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa25906
    Clone ID Related Accession (Same CDS sequence) NM_001162390.1 , XM_015277856.2 , XM_015277855.2
    Accession Version NM_001162390.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-22
    Organism Gallus gallus(chicken)
    Product collagen alpha-2(IV) chain precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000386.1. On Jun 21, 2009 this sequence version replaced XM_416952.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG GCTTTCAGGG CCCTCCAGGC TCTCCTGGGT TGCCACCAAT TCCTCCAAGG
    CTGGTTGCTG AGCAAGGTTC ACCAGGTCCC CGTGGAAATG CAGGGCCTCG GGGAAGTCCG
    GGTGATGCAG GACCTCAAGG TCCACCAGGA GAACCAGGTC TCAGAGGCTT ACCTGGAGAA
    CCAGGACTCC AGGGCAGAGG TGGCATTCCA GCTCCTCCTG GATCCAGAGG TGAACAAGGA
    GCAATGGGGT TTCAAGGTCC AGTGGGATTT GAAGGCCAGC CAGGTCGTCC TGGTAGCCCT
    GGGCTGCCAG GCATGCCGGG TCGCAGCGTT AGCATCGGCT ACCTGCTGGT GAAGCACAGC
    CAGTCCGACC AAGAGCCAAT GTGCCCGATT GGCATGAACA AACTCTGGAG TGGCTACAGC
    CTCCTGTACT TTGAGGGACA AGAGAAAGCA CACAACCAGG ACCTAGGGCT GGCTGGCTCA
    TGCTTGGCTC GTTTCAGTAC AATGCCATTC CTCTACTGTA ACCCTGGGGA TATTTGTTAT
    TATGCAAATC GAAATGACAA ATCCTACTGG CTCTCAACTA CAGCACCTCT TCCAATGATG
    CCTGTGGCAG AAGAAGAAAT TAGGCCATAT ATCAGCCGCT GCTCTGTGTG TGAGGCCCCA
    GCTGTGGCAA TTGCTGTCCA TAGCCAAGAA GCTTCTATTC CTCGCTGCCC TGAAGGCTGG
    CGCAGTTTAT GGATTGGATA TTCCTTCCTT ATGCACACTG CGGCTGGTGA TGAAGGCGGA
    GGACAGTCGC TGGTCTCTCC TGGGAGCTGC CTGGAAGATT TCAGGGCTAC TCCTTTCATC
    GAGTGCAATG GGGCCCGTGG AACCTGTCAT TACTTTGCCA ATAAGTACAG CTTCTGGCTC
    ACTACGATTG ACCAGCCCTT CCAAAGCAAG CCATCGGCAG ACACACTCAA GGCAGGACTC
    ATCCGAAGCC ACATCAGCAG ATGTCAAGTC TGTATGAAAA ATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001155862.1
    CDS51..5198
    Translation

    Target ORF information:

    RefSeq Version NM_001162390.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 2 chain (COL4A2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001162390.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG GCTTTCAGGG CCCTCCAGGC TCTCCTGGGT TGCCACCAAT TCCTCCAAGG
    CTGGTTGCTG AGCAAGGTTC ACCAGGTCCC CGTGGAAATG CAGGGCCTCG GGGAAGTCCG
    GGTGATGCAG GACCTCAAGG TCCACCAGGA GAACCAGGTC TCAGAGGCTT ACCTGGAGAA
    CCAGGACTCC AGGGCAGAGG TGGCATTCCA GCTCCTCCTG GATCCAGAGG TGAACAAGGA
    GCAATGGGGT TTCAAGGTCC AGTGGGATTT GAAGGCCAGC CAGGTCGTCC TGGTAGCCCT
    GGGCTGCCAG GCATGCCGGG TCGCAGCGTT AGCATCGGCT ACCTGCTGGT GAAGCACAGC
    CAGTCCGACC AAGAGCCAAT GTGCCCGATT GGCATGAACA AACTCTGGAG TGGCTACAGC
    CTCCTGTACT TTGAGGGACA AGAGAAAGCA CACAACCAGG ACCTAGGGCT GGCTGGCTCA
    TGCTTGGCTC GTTTCAGTAC AATGCCATTC CTCTACTGTA ACCCTGGGGA TATTTGTTAT
    TATGCAAATC GAAATGACAA ATCCTACTGG CTCTCAACTA CAGCACCTCT TCCAATGATG
    CCTGTGGCAG AAGAAGAAAT TAGGCCATAT ATCAGCCGCT GCTCTGTGTG TGAGGCCCCA
    GCTGTGGCAA TTGCTGTCCA TAGCCAAGAA GCTTCTATTC CTCGCTGCCC TGAAGGCTGG
    CGCAGTTTAT GGATTGGATA TTCCTTCCTT ATGCACACTG CGGCTGGTGA TGAAGGCGGA
    GGACAGTCGC TGGTCTCTCC TGGGAGCTGC CTGGAAGATT TCAGGGCTAC TCCTTTCATC
    GAGTGCAATG GGGCCCGTGG AACCTGTCAT TACTTTGCCA ATAAGTACAG CTTCTGGCTC
    ACTACGATTG ACCAGCCCTT CCAAAGCAAG CCATCGGCAG ACACACTCAA GGCAGGACTC
    ATCCGAAGCC ACATCAGCAG ATGTCAAGTC TGTATGAAAA ATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa25906
    Clone ID Related Accession (Same CDS sequence) NM_001162390.1 , XM_015277856.2 , XM_015277855.2
    Accession Version XM_015277856.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-2(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG GCTTTCAGGG CCCTCCAGGC TCTCCTGGGT TGCCACCAAT TCCTCCAAGG
    CTGGTTGCTG AGCAAGGTTC ACCAGGTCCC CGTGGAAATG CAGGGCCTCG GGGAAGTCCG
    GGTGATGCAG GACCTCAAGG TCCACCAGGA GAACCAGGTC TCAGAGGCTT ACCTGGAGAA
    CCAGGACTCC AGGGCAGAGG TGGCATTCCA GCTCCTCCTG GATCCAGAGG TGAACAAGGA
    GCAATGGGGT TTCAAGGTCC AGTGGGATTT GAAGGCCAGC CAGGTCGTCC TGGTAGCCCT
    GGGCTGCCAG GCATGCCGGG TCGCAGCGTT AGCATCGGCT ACCTGCTGGT GAAGCACAGC
    CAGTCCGACC AAGAGCCAAT GTGCCCGATT GGCATGAACA AACTCTGGAG TGGCTACAGC
    CTCCTGTACT TTGAGGGACA AGAGAAAGCA CACAACCAGG ACCTAGGGCT GGCTGGCTCA
    TGCTTGGCTC GTTTCAGTAC AATGCCATTC CTCTACTGTA ACCCTGGGGA TATTTGTTAT
    TATGCAAATC GAAATGACAA ATCCTACTGG CTCTCAACTA CAGCACCTCT TCCAATGATG
    CCTGTGGCAG AAGAAGAAAT TAGGCCATAT ATCAGCCGCT GCTCTGTGTG TGAGGCCCCA
    GCTGTGGCAA TTGCTGTCCA TAGCCAAGAA GCTTCTATTC CTCGCTGCCC TGAAGGCTGG
    CGCAGTTTAT GGATTGGATA TTCCTTCCTT ATGCACACTG CGGCTGGTGA TGAAGGCGGA
    GGACAGTCGC TGGTCTCTCC TGGGAGCTGC CTGGAAGATT TCAGGGCTAC TCCTTTCATC
    GAGTGCAATG GGGCCCGTGG AACCTGTCAT TACTTTGCCA ATAAGTACAG CTTCTGGCTC
    ACTACGATTG ACCAGCCCTT CCAAAGCAAG CCATCGGCAG ACACACTCAA GGCAGGACTC
    ATCCGAAGCC ACATCAGCAG ATGTCAAGTC TGTATGAAAA ATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133342.2
    CDS108..5255
    Translation

    Target ORF information:

    RefSeq Version XM_015277856.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 2 chain (COL4A2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277856.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG GCTTTCAGGG CCCTCCAGGC TCTCCTGGGT TGCCACCAAT TCCTCCAAGG
    CTGGTTGCTG AGCAAGGTTC ACCAGGTCCC CGTGGAAATG CAGGGCCTCG GGGAAGTCCG
    GGTGATGCAG GACCTCAAGG TCCACCAGGA GAACCAGGTC TCAGAGGCTT ACCTGGAGAA
    CCAGGACTCC AGGGCAGAGG TGGCATTCCA GCTCCTCCTG GATCCAGAGG TGAACAAGGA
    GCAATGGGGT TTCAAGGTCC AGTGGGATTT GAAGGCCAGC CAGGTCGTCC TGGTAGCCCT
    GGGCTGCCAG GCATGCCGGG TCGCAGCGTT AGCATCGGCT ACCTGCTGGT GAAGCACAGC
    CAGTCCGACC AAGAGCCAAT GTGCCCGATT GGCATGAACA AACTCTGGAG TGGCTACAGC
    CTCCTGTACT TTGAGGGACA AGAGAAAGCA CACAACCAGG ACCTAGGGCT GGCTGGCTCA
    TGCTTGGCTC GTTTCAGTAC AATGCCATTC CTCTACTGTA ACCCTGGGGA TATTTGTTAT
    TATGCAAATC GAAATGACAA ATCCTACTGG CTCTCAACTA CAGCACCTCT TCCAATGATG
    CCTGTGGCAG AAGAAGAAAT TAGGCCATAT ATCAGCCGCT GCTCTGTGTG TGAGGCCCCA
    GCTGTGGCAA TTGCTGTCCA TAGCCAAGAA GCTTCTATTC CTCGCTGCCC TGAAGGCTGG
    CGCAGTTTAT GGATTGGATA TTCCTTCCTT ATGCACACTG CGGCTGGTGA TGAAGGCGGA
    GGACAGTCGC TGGTCTCTCC TGGGAGCTGC CTGGAAGATT TCAGGGCTAC TCCTTTCATC
    GAGTGCAATG GGGCCCGTGG AACCTGTCAT TACTTTGCCA ATAAGTACAG CTTCTGGCTC
    ACTACGATTG ACCAGCCCTT CCAAAGCAAG CCATCGGCAG ACACACTCAA GGCAGGACTC
    ATCCGAAGCC ACATCAGCAG ATGTCAAGTC TGTATGAAAA ATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa25906
    Clone ID Related Accession (Same CDS sequence) NM_001162390.1 , XM_015277856.2 , XM_015277855.2
    Accession Version XM_015277855.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-2(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG GCTTTCAGGG CCCTCCAGGC TCTCCTGGGT TGCCACCAAT TCCTCCAAGG
    CTGGTTGCTG AGCAAGGTTC ACCAGGTCCC CGTGGAAATG CAGGGCCTCG GGGAAGTCCG
    GGTGATGCAG GACCTCAAGG TCCACCAGGA GAACCAGGTC TCAGAGGCTT ACCTGGAGAA
    CCAGGACTCC AGGGCAGAGG TGGCATTCCA GCTCCTCCTG GATCCAGAGG TGAACAAGGA
    GCAATGGGGT TTCAAGGTCC AGTGGGATTT GAAGGCCAGC CAGGTCGTCC TGGTAGCCCT
    GGGCTGCCAG GCATGCCGGG TCGCAGCGTT AGCATCGGCT ACCTGCTGGT GAAGCACAGC
    CAGTCCGACC AAGAGCCAAT GTGCCCGATT GGCATGAACA AACTCTGGAG TGGCTACAGC
    CTCCTGTACT TTGAGGGACA AGAGAAAGCA CACAACCAGG ACCTAGGGCT GGCTGGCTCA
    TGCTTGGCTC GTTTCAGTAC AATGCCATTC CTCTACTGTA ACCCTGGGGA TATTTGTTAT
    TATGCAAATC GAAATGACAA ATCCTACTGG CTCTCAACTA CAGCACCTCT TCCAATGATG
    CCTGTGGCAG AAGAAGAAAT TAGGCCATAT ATCAGCCGCT GCTCTGTGTG TGAGGCCCCA
    GCTGTGGCAA TTGCTGTCCA TAGCCAAGAA GCTTCTATTC CTCGCTGCCC TGAAGGCTGG
    CGCAGTTTAT GGATTGGATA TTCCTTCCTT ATGCACACTG CGGCTGGTGA TGAAGGCGGA
    GGACAGTCGC TGGTCTCTCC TGGGAGCTGC CTGGAAGATT TCAGGGCTAC TCCTTTCATC
    GAGTGCAATG GGGCCCGTGG AACCTGTCAT TACTTTGCCA ATAAGTACAG CTTCTGGCTC
    ACTACGATTG ACCAGCCCTT CCAAAGCAAG CCATCGGCAG ACACACTCAA GGCAGGACTC
    ATCCGAAGCC ACATCAGCAG ATGTCAAGTC TGTATGAAAA ATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133341.2
    CDS145..5292
    Translation

    Target ORF information:

    RefSeq Version XM_015277855.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 2 chain (COL4A2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277855.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG GCTTTCAGGG CCCTCCAGGC TCTCCTGGGT TGCCACCAAT TCCTCCAAGG
    CTGGTTGCTG AGCAAGGTTC ACCAGGTCCC CGTGGAAATG CAGGGCCTCG GGGAAGTCCG
    GGTGATGCAG GACCTCAAGG TCCACCAGGA GAACCAGGTC TCAGAGGCTT ACCTGGAGAA
    CCAGGACTCC AGGGCAGAGG TGGCATTCCA GCTCCTCCTG GATCCAGAGG TGAACAAGGA
    GCAATGGGGT TTCAAGGTCC AGTGGGATTT GAAGGCCAGC CAGGTCGTCC TGGTAGCCCT
    GGGCTGCCAG GCATGCCGGG TCGCAGCGTT AGCATCGGCT ACCTGCTGGT GAAGCACAGC
    CAGTCCGACC AAGAGCCAAT GTGCCCGATT GGCATGAACA AACTCTGGAG TGGCTACAGC
    CTCCTGTACT TTGAGGGACA AGAGAAAGCA CACAACCAGG ACCTAGGGCT GGCTGGCTCA
    TGCTTGGCTC GTTTCAGTAC AATGCCATTC CTCTACTGTA ACCCTGGGGA TATTTGTTAT
    TATGCAAATC GAAATGACAA ATCCTACTGG CTCTCAACTA CAGCACCTCT TCCAATGATG
    CCTGTGGCAG AAGAAGAAAT TAGGCCATAT ATCAGCCGCT GCTCTGTGTG TGAGGCCCCA
    GCTGTGGCAA TTGCTGTCCA TAGCCAAGAA GCTTCTATTC CTCGCTGCCC TGAAGGCTGG
    CGCAGTTTAT GGATTGGATA TTCCTTCCTT ATGCACACTG CGGCTGGTGA TGAAGGCGGA
    GGACAGTCGC TGGTCTCTCC TGGGAGCTGC CTGGAAGATT TCAGGGCTAC TCCTTTCATC
    GAGTGCAATG GGGCCCGTGG AACCTGTCAT TACTTTGCCA ATAAGTACAG CTTCTGGCTC
    ACTACGATTG ACCAGCCCTT CCAAAGCAAG CCATCGGCAG ACACACTCAA GGCAGGACTC
    ATCCGAAGCC ACATCAGCAG ATGTCAAGTC TGTATGAAAA ATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30679
    Clone ID Related Accession (Same CDS sequence) XM_015277856.1 , XM_015277855.1
    Accession Version XM_015277856.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-2(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG GCTTTCAGGG CCCTCCAGGC TCTCCTGGGT TGCCACCAAT TCCTCCAAGG
    CTGGTTGCTG AGCAAGGTTC ACCAGGTCCC CGTGGAAATG CAGGGCCTCG GGGAAGTCCG
    GGTGATGCAG GACCTCAAGG TCCACCAGGA GAACCAGGTC TCAGAGGCTT ACCTGGAGAA
    CCAGGACTCC AGGGCAGAGG TGGCATTCCA GCTCCTCCTG GATCCAGAGG TGAACAAGGA
    GCAATGGGGT TTCAAGGTCC AGTGGGATTT GAAGGCCAGC CAGGTCGTCC TGGTAGCCCT
    GGGCTGCCAG GCATGCCGGG TCGCAGCGTT AGCATCGGCT ACCTGCTGGT GAAGCACAGC
    CAGTCCGACC AAGAGCCAAT GTGTCCCATT GGCATGAACA AGCTCTGGGA TGGCTACAGC
    CTCCTGTACT TTGAGGGACA AGAGAAAGCA CACAACCAGG ACCTAGGGCT GGCTGGCTCA
    TGCTTGGCTC GTTTCAGTAC AATGCCATTC CTCTACTGTA ACCCTGGGGA TATTTGTTAT
    TATGCAAATC GAAATGACAA ATCCTACTGG CTCTCAACTA CAGCACCTCT TCCAATGATG
    CCTGTGGCAG AAGAAGAAAT TAGGCCATAT ATCAGCCGCT GCTCTGTGTG TGAGGCCCCA
    GCTGTGGCAA TTGCTGTCCA TAGCCAAGAA GCTTCTATTC CTCGCTGCCC TGAAGGCTGG
    CGCAGTTTAT GGATTGGATA TTCCTTCCTT ATGCACACTG CGGCTGGTGA TGAAGGCGGA
    GGACAGTCGC TGGTCTCTCC TGGGAGCTGC CTGGAAGATT TCAGGGCTAC TCCTTTCATC
    GAGTGCAATG GGGCCCGTGG AACCTGTCAT TACTTTGCCA ATAAGTACAG CTTCTGGCTC
    ACTACGATTG ACCAGCCCTT CCAAAGCAAG CCATCGGCAG ACACACTCAA GGCAGGACTC
    ATCCGAAGCC ACATCAGCAG ATGTCAAGTC TGTATGAAAA ATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133342.1
    CDS130..5277
    Translation

    Target ORF information:

    RefSeq Version XM_015277856.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type IV, alpha 2 (COL4A2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277856.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG GCTTTCAGGG CCCTCCAGGC TCTCCTGGGT TGCCACCAAT TCCTCCAAGG
    CTGGTTGCTG AGCAAGGTTC ACCAGGTCCC CGTGGAAATG CAGGGCCTCG GGGAAGTCCG
    GGTGATGCAG GACCTCAAGG TCCACCAGGA GAACCAGGTC TCAGAGGCTT ACCTGGAGAA
    CCAGGACTCC AGGGCAGAGG TGGCATTCCA GCTCCTCCTG GATCCAGAGG TGAACAAGGA
    GCAATGGGGT TTCAAGGTCC AGTGGGATTT GAAGGCCAGC CAGGTCGTCC TGGTAGCCCT
    GGGCTGCCAG GCATGCCGGG TCGCAGCGTT AGCATCGGCT ACCTGCTGGT GAAGCACAGC
    CAGTCCGACC AAGAGCCAAT GTGTCCCATT GGCATGAACA AGCTCTGGGA TGGCTACAGC
    CTCCTGTACT TTGAGGGACA AGAGAAAGCA CACAACCAGG ACCTAGGGCT GGCTGGCTCA
    TGCTTGGCTC GTTTCAGTAC AATGCCATTC CTCTACTGTA ACCCTGGGGA TATTTGTTAT
    TATGCAAATC GAAATGACAA ATCCTACTGG CTCTCAACTA CAGCACCTCT TCCAATGATG
    CCTGTGGCAG AAGAAGAAAT TAGGCCATAT ATCAGCCGCT GCTCTGTGTG TGAGGCCCCA
    GCTGTGGCAA TTGCTGTCCA TAGCCAAGAA GCTTCTATTC CTCGCTGCCC TGAAGGCTGG
    CGCAGTTTAT GGATTGGATA TTCCTTCCTT ATGCACACTG CGGCTGGTGA TGAAGGCGGA
    GGACAGTCGC TGGTCTCTCC TGGGAGCTGC CTGGAAGATT TCAGGGCTAC TCCTTTCATC
    GAGTGCAATG GGGCCCGTGG AACCTGTCAT TACTTTGCCA ATAAGTACAG CTTCTGGCTC
    ACTACGATTG ACCAGCCCTT CCAAAGCAAG CCATCGGCAG ACACACTCAA GGCAGGACTC
    ATCCGAAGCC ACATCAGCAG ATGTCAAGTC TGTATGAAAA ATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30679
    Clone ID Related Accession (Same CDS sequence) XM_015277856.1 , XM_015277855.1
    Accession Version XM_015277855.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-2(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG GCTTTCAGGG CCCTCCAGGC TCTCCTGGGT TGCCACCAAT TCCTCCAAGG
    CTGGTTGCTG AGCAAGGTTC ACCAGGTCCC CGTGGAAATG CAGGGCCTCG GGGAAGTCCG
    GGTGATGCAG GACCTCAAGG TCCACCAGGA GAACCAGGTC TCAGAGGCTT ACCTGGAGAA
    CCAGGACTCC AGGGCAGAGG TGGCATTCCA GCTCCTCCTG GATCCAGAGG TGAACAAGGA
    GCAATGGGGT TTCAAGGTCC AGTGGGATTT GAAGGCCAGC CAGGTCGTCC TGGTAGCCCT
    GGGCTGCCAG GCATGCCGGG TCGCAGCGTT AGCATCGGCT ACCTGCTGGT GAAGCACAGC
    CAGTCCGACC AAGAGCCAAT GTGTCCCATT GGCATGAACA AGCTCTGGGA TGGCTACAGC
    CTCCTGTACT TTGAGGGACA AGAGAAAGCA CACAACCAGG ACCTAGGGCT GGCTGGCTCA
    TGCTTGGCTC GTTTCAGTAC AATGCCATTC CTCTACTGTA ACCCTGGGGA TATTTGTTAT
    TATGCAAATC GAAATGACAA ATCCTACTGG CTCTCAACTA CAGCACCTCT TCCAATGATG
    CCTGTGGCAG AAGAAGAAAT TAGGCCATAT ATCAGCCGCT GCTCTGTGTG TGAGGCCCCA
    GCTGTGGCAA TTGCTGTCCA TAGCCAAGAA GCTTCTATTC CTCGCTGCCC TGAAGGCTGG
    CGCAGTTTAT GGATTGGATA TTCCTTCCTT ATGCACACTG CGGCTGGTGA TGAAGGCGGA
    GGACAGTCGC TGGTCTCTCC TGGGAGCTGC CTGGAAGATT TCAGGGCTAC TCCTTTCATC
    GAGTGCAATG GGGCCCGTGG AACCTGTCAT TACTTTGCCA ATAAGTACAG CTTCTGGCTC
    ACTACGATTG ACCAGCCCTT CCAAAGCAAG CCATCGGCAG ACACACTCAA GGCAGGACTC
    ATCCGAAGCC ACATCAGCAG ATGTCAAGTC TGTATGAAAA ATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133341.1
    CDS143..5290
    Translation

    Target ORF information:

    RefSeq Version XM_015277855.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type IV, alpha 2 (COL4A2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277855.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG GCTTTCAGGG CCCTCCAGGC TCTCCTGGGT TGCCACCAAT TCCTCCAAGG
    CTGGTTGCTG AGCAAGGTTC ACCAGGTCCC CGTGGAAATG CAGGGCCTCG GGGAAGTCCG
    GGTGATGCAG GACCTCAAGG TCCACCAGGA GAACCAGGTC TCAGAGGCTT ACCTGGAGAA
    CCAGGACTCC AGGGCAGAGG TGGCATTCCA GCTCCTCCTG GATCCAGAGG TGAACAAGGA
    GCAATGGGGT TTCAAGGTCC AGTGGGATTT GAAGGCCAGC CAGGTCGTCC TGGTAGCCCT
    GGGCTGCCAG GCATGCCGGG TCGCAGCGTT AGCATCGGCT ACCTGCTGGT GAAGCACAGC
    CAGTCCGACC AAGAGCCAAT GTGTCCCATT GGCATGAACA AGCTCTGGGA TGGCTACAGC
    CTCCTGTACT TTGAGGGACA AGAGAAAGCA CACAACCAGG ACCTAGGGCT GGCTGGCTCA
    TGCTTGGCTC GTTTCAGTAC AATGCCATTC CTCTACTGTA ACCCTGGGGA TATTTGTTAT
    TATGCAAATC GAAATGACAA ATCCTACTGG CTCTCAACTA CAGCACCTCT TCCAATGATG
    CCTGTGGCAG AAGAAGAAAT TAGGCCATAT ATCAGCCGCT GCTCTGTGTG TGAGGCCCCA
    GCTGTGGCAA TTGCTGTCCA TAGCCAAGAA GCTTCTATTC CTCGCTGCCC TGAAGGCTGG
    CGCAGTTTAT GGATTGGATA TTCCTTCCTT ATGCACACTG CGGCTGGTGA TGAAGGCGGA
    GGACAGTCGC TGGTCTCTCC TGGGAGCTGC CTGGAAGATT TCAGGGCTAC TCCTTTCATC
    GAGTGCAATG GGGCCCGTGG AACCTGTCAT TACTTTGCCA ATAAGTACAG CTTCTGGCTC
    ACTACGATTG ACCAGCCCTT CCAAAGCAAG CCATCGGCAG ACACACTCAA GGCAGGACTC
    ATCCGAAGCC ACATCAGCAG ATGTCAAGTC TGTATGAAAA ATTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30680
    Clone ID Related Accession (Same CDS sequence) XM_015277857.1
    Accession Version XM_015277857.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-2(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGATCC CAGGACTGCA AGGCCCTAAA GGTCACAAGG GTGAAAGAGG ACATCCAGGA 
    ATAAGTGGAC CCAAAGGAGA AACGGGGCAA AGAGGAGTCA CTGGTTTCCC AGGGGCAGAT
    GGCGTTCCTG GTCATCCAGG GCAGCCAGGA TCAAGGGGGA AGCCAGGTCA TGATGGCTGT
    AATGGGACAG TAGGTGACCC AGGTGACCCA GGAACACCAG GACACTCTGG TTTCCCTGGT
    ACCATTGGAG TTCAAGGACC AAAGGGCCAG AAGGGGGAGC CCTATGTGCT ACCGCCAGAC
    ATCGCCAGCC GACATCGGGG GGATCCTGGG GACCCAGGAT TCACTGGTTT TCCGGGAGCT
    CCGGGTACAT TGGGTATTCA AGGACCTATT GGTCCAAGAG GAGTGCCAGG AAGACCTGGA
    CCACCGGGGT CCCCAGGACC GCAAGGACCA CAAGGCAACA GAGGTCTTGG CTTCTATGGC
    GAAAAAGGTG AACAGGGCTC CCCAGGTCCT CCAGGTCCAC CTGGACTTCC CACAAGAGAA
    CTGATTGGTG TCCCCACTGA CAAACACAAA GGTGAAAGAG GAGAGCCTGG ACAAAAAGGG
    GAAGCTGGAT TTCCAGGTGT ACTTCTCTTC GCTCCTGAAA AAGGTGAAGA AGGGGTAATG
    GGCTTCCCAG GACAAAGGGG ACTGCCTGGT AACGATGGTT TTCCAGGACT CAGTGGAGAA
    AGAGGTTTTC CAGGATTTGA TGGTCAACCA GGTCAATATG GTCCTAGAGG AGGCAAGGGG
    GAGCAGGGTG AGATGGGTCC TCCAGGACCA CCTGCCTATG TTCCTTACCG CATTCCAAGA
    AAAGGTGTAA GAGGTGATCC AGGCTCTCCA GGTGCTTCTG GACTACGGGG AGAACAAGGA
    GAGCAGGGTG ATCGTGGGCT GCCTGGTATT CCAGGATTCT CTGATGGAGA TGCAGATAAG
    CCAGGCTTAC CTGGAGAAAT AGGACCAAAA GGTGAAAAAG GTGAAGAAGG ATCTCCTGCT
    TATCAAGCAG GCCCGCCTGG GTTTCCAGGT AAACACGGTG AACCAGGAAT CCGTGGTCCA
    CCTGGTCCCC CTGGAACTCC AGGTTCTCTC TTCGGATTAA AAGGACAGGA AGGGACACCA
    GGTAGACCTG GTGTACAAGG TTTTCCAGGG CCAAGAGGAC AAAGAGGACC TAAAGGTGAA
    GAAGGTGACT GCAGTAAATG CCTTCTGAGT GATGAACTCA GAAGGGGCTC TACTGGCCCC
    CGTGGTCCTC CTGGTTTTCC AGGAACCCCT GGCCAGCCTG GGAGAAAAGG AGAACCTGGT
    GATCAAGGTC CACATGGTAT TCCTGGCTAT GCTGGAGCAA AAGGACAATC GGGCCAAGAA
    GGATTACCTG GACCAAAAGG AGAAAAGGGA GATTCTATAT ACATAACTAC TAAAGGCACC
    AAAGGAATCC GAGGAGATCC TGGACTTCCT GGTATCAGAG GGGAAGATGG TTTCCCAGGC
    AGAGATGGAT TAGATGGTTT ACCAGGACTG CCTGGCCTTC CGGGTGATGG TATCAGAGGT
    CTCCCCGGGG ATCCTGGTTA TCCAGGAGAA TTAGGCCCAA AGGGCTTTCC AGGAGAAATA
    GGCCTACCAG GTGAAGGATA TCCTGGCCCA AAAGGATACC GTGGTCTTCC AGGTGACCGT
    GGAACAGATG GACACCCTGG GCCTCCAGGT CTGCCTGGTC CACCAGGAGA GCCTGGCCAA
    TTAGACTGCG GGCAGGTGAT TGAAGATTTT TCTAGAGGAG AAGCAACTGA TCCAATATGG
    TCAGGTGGAG GCTGCGTGAG GCCACCAAAG GGATCTCAAG GCAACCCAGG GCTGCCAGGA
    GCCACAGGTA CTAAAGGTGC AAGAGGATTC CCGGGTGATC CAGGCCCAGT GGGATTCCCA
    GGGCTAAATG GTACACGAGG TGATCCAGGT CGGGAAGGAT ATCCAGGTCC TCCAGGCTTT
    ATTGGGCCCC GGGGAGACAG AGGCCCAAAT GGCCTACCTG GACTACAGGG ACATCCAGGT
    CTTATGGGAA AGTCTGGTGC TCCAGGACTT GCTGGACAGA AGGGTGCTCC TGGTGACGTT
    CTGGGAGCAG CAGCAGGTCC TCGAGGGGAT GATGGGCTGC CTGGCTTCCC TGGCTTGAAA
    GGTGCACCAG GAGATCAAGG AATACCAGGA ATTAGAGGAG CGGACGGTAA CCCAGGATTG
    CCAGGCCCCA AAGGTGACCC AGGGCTGCAA GGTCTCCCTG GTTTGATGGG TTTGCCAGGA
    ACACCAGGAA CTCATGGATT CCCAGGGCCA CCAGGAAACC GCGGCCCTGA CGGTGGACCC
    GGCTCTCAAG GACCCCTTGG TCCACCAGGT GCAAGGGGAG AAGATGGCGA GCAAGGCTTT
    CCTGGTCCCG TGGGCATGAA AGGTCTGTCT GGTGACAAAG GTGAAACAGG TTTCCCAGGA
    TTACAAGGTA TCCCTGGTGT GACCGGTCCC CCTGGCATTA GTGGGATGGA TGGCTTCCCA
    GGAGACAAAG GCTCAAGAGG TTCACCTGGC ATTGATGGTT TCAAAGGCAT GCCAGGCCTG
    AAAGGAAGAC CAGGAATCAA AGGAATCAAA GGAGAATTTG GCTTACTTGG GACTCGGGGT
    GATAAAGGCG CACAGGGTGC ACGAGGCTTT AAAGGTGACC GTGGAGAGCA AGGTCCCCCA
    GGAGAGCCCC CGAAGCTGAA GCCTAGCATG ATGATGGAAG TTAAAGGTGA AAAAGGAGAT
    GCTGGAGAAA CTGGCACCAA GGGATTCTTT GGCATAAAAG GTAGCAAGGG AATGCCAGGC
    CTCCCCGGTA AGACAGGAAT TCCTGGGTCC CCAGGGCACC CCAGTTATGT GCCTGGTGTG
    AAGGGGGACA TCGGTGCAAA AGGGCTGACG GGTCTGAAAG GGTATCCTGG TCCGACTGGC
    TCTCCAGGCA TCAGAGGCTT CCCTGGCAGC ACAGGAGGCA GGGGAGATAA GGGTGCCCCA
    GGAATTTCGG GCCATTTTGG TACTCCTGGC TCCCATGGTG AAATTGGTGA GCCGGGCGAT
    ACTATAAACT TACCAGGAAT GCCAGGCCTC AAAGGGGAAG TTGGTGTGCC GGGTCTTACA
    GGCTTAAGAG GAGGCCCTGG ACAAAAAGGT GAAGGAGGTG ATCCTGGTTT ACCTGGCATT
    GAAGGCCTCA AGGGCATACA GGGTGTACCT GGCTCCTTGG GACAGAAAGG TTTGCCTGGA
    CTGGTCGGCC CTCCAGGGCA GCAAGGAAGC CCCGGGACGC CTGGATTCCA AGGAGAAAAA
    GGAGCTCCCG GCTGGCCTGG CTTACCAGGA CAAGCAGGCT TGCCTGGCTT AAGAGGAATC
    AGTGGACTGC ATGGTTTACC GGGCACCAAG GGCTTACCAG GATCACCAGG TCCAGACGGC
    TATGGCTCTG CGGGCTTCCC AGGCCCTGTG GGTGACAAAG GAGAAGCAGG AGAGCCCAGC
    AGAGTGGAGG GCAGCCAAGG CCCGCCGGGT CAGAAGGGAG ACAGAGGTGT TCCAGGAGTC
    CAAGGACCCT TTGGCATTCC TGGGCAGGAT GGACTTCCAG GGCCTCCTGG GATTTCCAAT
    ATATCAGGAT ATCCAGGCGA TACAGGTTCC CCAGGTCTAG ATGGAGTGCC AGGCTATCCA
    GGACTACATG GGCAGCCTGG AATACCAGCT CCACCGGGAA GCAAGGGAGA AAGTGGACGA
    GCAGGTGTGA GCGGACAAGC TGGCCCCAAA GGGACAAGAG GTGATCCTGG ATTACCAGGG
    AGACCTGGGA TTCCTGGTTA TCCTGGACCA AAAGGTCGTA AGGGTGAACA AGGTGTCATT
    GGCTTTATTG GCACAGTAGG ATTTCCAGGT GATTTGGGAC CCATCGGACC CAAGGGGGAT
    AGAGGGCTTA CTGGCTTTCA GGGCCCTCCA GGCTCTCCTG GGTTGCCACC AATTCCTCCA
    AGGCTGGTTG CTGAGCAAGG TTCACCAGGT CCCCGTGGAA ATGCAGGGCC TCGGGGAAGT
    CCGGGTGATG CAGGACCTCA AGGTCCACCA GGAGAACCAG GTCTCAGAGG CTTACCTGGA
    GAACCAGGAC TCCAGGGCAG AGGTGGCATT CCAGCTCCTC CTGGATCCAG AGGTGAACAA
    GGAGCAATGG GGTTTCAAGG TCCAGTGGGA TTTGAAGGCC AGCCAGGTCG TCCTGGTAGC
    CCTGGGCTGC CAGGCATGCC GGGTCGCAGC GTTAGCATCG GCTACCTGCT GGTGAAGCAC
    AGCCAGTCCG ACCAAGAGCC AATGTGTCCC ATTGGCATGA ACAAGCTCTG GGATGGCTAC
    AGCCTCCTGT ACTTTGAGGG ACAAGAGAAA GCACACAACC AGGACCTAGG GCTGGCTGGC
    TCATGCTTGG CTCGTTTCAG TACAATGCCA TTCCTCTACT GTAACCCTGG GGATATTTGT
    TATTATGCAA ATCGAAATGA CAAATCCTAC TGGCTCTCAA CTACAGCACC TCTTCCAATG
    ATGCCTGTGG CAGAAGAAGA AATTAGGCCA TATATCAGCC GCTGCTCTGT GTGTGAGGCC
    CCAGCTGTGG CAATTGCTGT CCATAGCCAA GAAGCTTCTA TTCCTCGCTG CCCTGAAGGC
    TGGCGCAGTT TATGGATTGG ATATTCCTTC CTTATGCACA CTGCGGCTGG TGATGAAGGC
    GGAGGACAGT CGCTGGTCTC TCCTGGGAGC TGCCTGGAAG ATTTCAGGGC TACTCCTTTC
    ATCGAGTGCA ATGGGGCCCG TGGAACCTGT CATTACTTTG CCAATAAGTA CAGCTTCTGG
    CTCACTACGA TTGACCAGCC CTTCCAAAGC AAGCCATCGG CAGACACACT CAAGGCAGGA
    CTCATCCGAA GCCACATCAG CAGATGTCAA GTCTGTATGA AAAATTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133343.1
    CDS152..5062
    Translation

    Target ORF information:

    RefSeq Version XM_015277857.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type IV, alpha 2 (COL4A2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277857.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGATCC CAGGACTGCA AGGCCCTAAA GGTCACAAGG GTGAAAGAGG ACATCCAGGA 
    ATAAGTGGAC CCAAAGGAGA AACGGGGCAA AGAGGAGTCA CTGGTTTCCC AGGGGCAGAT
    GGCGTTCCTG GTCATCCAGG GCAGCCAGGA TCAAGGGGGA AGCCAGGTCA TGATGGCTGT
    AATGGGACAG TAGGTGACCC AGGTGACCCA GGAACACCAG GACACTCTGG TTTCCCTGGT
    ACCATTGGAG TTCAAGGACC AAAGGGCCAG AAGGGGGAGC CCTATGTGCT ACCGCCAGAC
    ATCGCCAGCC GACATCGGGG GGATCCTGGG GACCCAGGAT TCACTGGTTT TCCGGGAGCT
    CCGGGTACAT TGGGTATTCA AGGACCTATT GGTCCAAGAG GAGTGCCAGG AAGACCTGGA
    CCACCGGGGT CCCCAGGACC GCAAGGACCA CAAGGCAACA GAGGTCTTGG CTTCTATGGC
    GAAAAAGGTG AACAGGGCTC CCCAGGTCCT CCAGGTCCAC CTGGACTTCC CACAAGAGAA
    CTGATTGGTG TCCCCACTGA CAAACACAAA GGTGAAAGAG GAGAGCCTGG ACAAAAAGGG
    GAAGCTGGAT TTCCAGGTGT ACTTCTCTTC GCTCCTGAAA AAGGTGAAGA AGGGGTAATG
    GGCTTCCCAG GACAAAGGGG ACTGCCTGGT AACGATGGTT TTCCAGGACT CAGTGGAGAA
    AGAGGTTTTC CAGGATTTGA TGGTCAACCA GGTCAATATG GTCCTAGAGG AGGCAAGGGG
    GAGCAGGGTG AGATGGGTCC TCCAGGACCA CCTGCCTATG TTCCTTACCG CATTCCAAGA
    AAAGGTGTAA GAGGTGATCC AGGCTCTCCA GGTGCTTCTG GACTACGGGG AGAACAAGGA
    GAGCAGGGTG ATCGTGGGCT GCCTGGTATT CCAGGATTCT CTGATGGAGA TGCAGATAAG
    CCAGGCTTAC CTGGAGAAAT AGGACCAAAA GGTGAAAAAG GTGAAGAAGG ATCTCCTGCT
    TATCAAGCAG GCCCGCCTGG GTTTCCAGGT AAACACGGTG AACCAGGAAT CCGTGGTCCA
    CCTGGTCCCC CTGGAACTCC AGGTTCTCTC TTCGGATTAA AAGGACAGGA AGGGACACCA
    GGTAGACCTG GTGTACAAGG TTTTCCAGGG CCAAGAGGAC AAAGAGGACC TAAAGGTGAA
    GAAGGTGACT GCAGTAAATG CCTTCTGAGT GATGAACTCA GAAGGGGCTC TACTGGCCCC
    CGTGGTCCTC CTGGTTTTCC AGGAACCCCT GGCCAGCCTG GGAGAAAAGG AGAACCTGGT
    GATCAAGGTC CACATGGTAT TCCTGGCTAT GCTGGAGCAA AAGGACAATC GGGCCAAGAA
    GGATTACCTG GACCAAAAGG AGAAAAGGGA GATTCTATAT ACATAACTAC TAAAGGCACC
    AAAGGAATCC GAGGAGATCC TGGACTTCCT GGTATCAGAG GGGAAGATGG TTTCCCAGGC
    AGAGATGGAT TAGATGGTTT ACCAGGACTG CCTGGCCTTC CGGGTGATGG TATCAGAGGT
    CTCCCCGGGG ATCCTGGTTA TCCAGGAGAA TTAGGCCCAA AGGGCTTTCC AGGAGAAATA
    GGCCTACCAG GTGAAGGATA TCCTGGCCCA AAAGGATACC GTGGTCTTCC AGGTGACCGT
    GGAACAGATG GACACCCTGG GCCTCCAGGT CTGCCTGGTC CACCAGGAGA GCCTGGCCAA
    TTAGACTGCG GGCAGGTGAT TGAAGATTTT TCTAGAGGAG AAGCAACTGA TCCAATATGG
    TCAGGTGGAG GCTGCGTGAG GCCACCAAAG GGATCTCAAG GCAACCCAGG GCTGCCAGGA
    GCCACAGGTA CTAAAGGTGC AAGAGGATTC CCGGGTGATC CAGGCCCAGT GGGATTCCCA
    GGGCTAAATG GTACACGAGG TGATCCAGGT CGGGAAGGAT ATCCAGGTCC TCCAGGCTTT
    ATTGGGCCCC GGGGAGACAG AGGCCCAAAT GGCCTACCTG GACTACAGGG ACATCCAGGT
    CTTATGGGAA AGTCTGGTGC TCCAGGACTT GCTGGACAGA AGGGTGCTCC TGGTGACGTT
    CTGGGAGCAG CAGCAGGTCC TCGAGGGGAT GATGGGCTGC CTGGCTTCCC TGGCTTGAAA
    GGTGCACCAG GAGATCAAGG AATACCAGGA ATTAGAGGAG CGGACGGTAA CCCAGGATTG
    CCAGGCCCCA AAGGTGACCC AGGGCTGCAA GGTCTCCCTG GTTTGATGGG TTTGCCAGGA
    ACACCAGGAA CTCATGGATT CCCAGGGCCA CCAGGAAACC GCGGCCCTGA CGGTGGACCC
    GGCTCTCAAG GACCCCTTGG TCCACCAGGT GCAAGGGGAG AAGATGGCGA GCAAGGCTTT
    CCTGGTCCCG TGGGCATGAA AGGTCTGTCT GGTGACAAAG GTGAAACAGG TTTCCCAGGA
    TTACAAGGTA TCCCTGGTGT GACCGGTCCC CCTGGCATTA GTGGGATGGA TGGCTTCCCA
    GGAGACAAAG GCTCAAGAGG TTCACCTGGC ATTGATGGTT TCAAAGGCAT GCCAGGCCTG
    AAAGGAAGAC CAGGAATCAA AGGAATCAAA GGAGAATTTG GCTTACTTGG GACTCGGGGT
    GATAAAGGCG CACAGGGTGC ACGAGGCTTT AAAGGTGACC GTGGAGAGCA AGGTCCCCCA
    GGAGAGCCCC CGAAGCTGAA GCCTAGCATG ATGATGGAAG TTAAAGGTGA AAAAGGAGAT
    GCTGGAGAAA CTGGCACCAA GGGATTCTTT GGCATAAAAG GTAGCAAGGG AATGCCAGGC
    CTCCCCGGTA AGACAGGAAT TCCTGGGTCC CCAGGGCACC CCAGTTATGT GCCTGGTGTG
    AAGGGGGACA TCGGTGCAAA AGGGCTGACG GGTCTGAAAG GGTATCCTGG TCCGACTGGC
    TCTCCAGGCA TCAGAGGCTT CCCTGGCAGC ACAGGAGGCA GGGGAGATAA GGGTGCCCCA
    GGAATTTCGG GCCATTTTGG TACTCCTGGC TCCCATGGTG AAATTGGTGA GCCGGGCGAT
    ACTATAAACT TACCAGGAAT GCCAGGCCTC AAAGGGGAAG TTGGTGTGCC GGGTCTTACA
    GGCTTAAGAG GAGGCCCTGG ACAAAAAGGT GAAGGAGGTG ATCCTGGTTT ACCTGGCATT
    GAAGGCCTCA AGGGCATACA GGGTGTACCT GGCTCCTTGG GACAGAAAGG TTTGCCTGGA
    CTGGTCGGCC CTCCAGGGCA GCAAGGAAGC CCCGGGACGC CTGGATTCCA AGGAGAAAAA
    GGAGCTCCCG GCTGGCCTGG CTTACCAGGA CAAGCAGGCT TGCCTGGCTT AAGAGGAATC
    AGTGGACTGC ATGGTTTACC GGGCACCAAG GGCTTACCAG GATCACCAGG TCCAGACGGC
    TATGGCTCTG CGGGCTTCCC AGGCCCTGTG GGTGACAAAG GAGAAGCAGG AGAGCCCAGC
    AGAGTGGAGG GCAGCCAAGG CCCGCCGGGT CAGAAGGGAG ACAGAGGTGT TCCAGGAGTC
    CAAGGACCCT TTGGCATTCC TGGGCAGGAT GGACTTCCAG GGCCTCCTGG GATTTCCAAT
    ATATCAGGAT ATCCAGGCGA TACAGGTTCC CCAGGTCTAG ATGGAGTGCC AGGCTATCCA
    GGACTACATG GGCAGCCTGG AATACCAGCT CCACCGGGAA GCAAGGGAGA AAGTGGACGA
    GCAGGTGTGA GCGGACAAGC TGGCCCCAAA GGGACAAGAG GTGATCCTGG ATTACCAGGG
    AGACCTGGGA TTCCTGGTTA TCCTGGACCA AAAGGTCGTA AGGGTGAACA AGGTGTCATT
    GGCTTTATTG GCACAGTAGG ATTTCCAGGT GATTTGGGAC CCATCGGACC CAAGGGGGAT
    AGAGGGCTTA CTGGCTTTCA GGGCCCTCCA GGCTCTCCTG GGTTGCCACC AATTCCTCCA
    AGGCTGGTTG CTGAGCAAGG TTCACCAGGT CCCCGTGGAA ATGCAGGGCC TCGGGGAAGT
    CCGGGTGATG CAGGACCTCA AGGTCCACCA GGAGAACCAG GTCTCAGAGG CTTACCTGGA
    GAACCAGGAC TCCAGGGCAG AGGTGGCATT CCAGCTCCTC CTGGATCCAG AGGTGAACAA
    GGAGCAATGG GGTTTCAAGG TCCAGTGGGA TTTGAAGGCC AGCCAGGTCG TCCTGGTAGC
    CCTGGGCTGC CAGGCATGCC GGGTCGCAGC GTTAGCATCG GCTACCTGCT GGTGAAGCAC
    AGCCAGTCCG ACCAAGAGCC AATGTGTCCC ATTGGCATGA ACAAGCTCTG GGATGGCTAC
    AGCCTCCTGT ACTTTGAGGG ACAAGAGAAA GCACACAACC AGGACCTAGG GCTGGCTGGC
    TCATGCTTGG CTCGTTTCAG TACAATGCCA TTCCTCTACT GTAACCCTGG GGATATTTGT
    TATTATGCAA ATCGAAATGA CAAATCCTAC TGGCTCTCAA CTACAGCACC TCTTCCAATG
    ATGCCTGTGG CAGAAGAAGA AATTAGGCCA TATATCAGCC GCTGCTCTGT GTGTGAGGCC
    CCAGCTGTGG CAATTGCTGT CCATAGCCAA GAAGCTTCTA TTCCTCGCTG CCCTGAAGGC
    TGGCGCAGTT TATGGATTGG ATATTCCTTC CTTATGCACA CTGCGGCTGG TGATGAAGGC
    GGAGGACAGT CGCTGGTCTC TCCTGGGAGC TGCCTGGAAG ATTTCAGGGC TACTCCTTTC
    ATCGAGTGCA ATGGGGCCCG TGGAACCTGT CATTACTTTG CCAATAAGTA CAGCTTCTGG
    CTCACTACGA TTGACCAGCC CTTCCAAAGC AAGCCATCGG CAGACACACT CAAGGCAGGA
    CTCATCCGAA GCCACATCAG CAGATGTCAA GTCTGTATGA AAAATTTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30681
    Clone ID Related Accession (Same CDS sequence) XM_015277858.1 , XM_015277858.2
    Accession Version XM_015277858.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-2(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763585.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG AAGTCCATAA AAAGATGATG CTCCACCTGT TGACTTTTGG GGGGGGAACT
    TCCCAGCCTC CTACATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133344.1
    CDS226..4446
    Translation

    Target ORF information:

    RefSeq Version XM_015277858.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type IV, alpha 2 (COL4A2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277858.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG AAGTCCATAA AAAGATGATG CTCCACCTGT TGACTTTTGG GGGGGGAACT
    TCCCAGCCTC CTACATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30681
    Clone ID Related Accession (Same CDS sequence) XM_015277858.1 , XM_015277858.2
    Accession Version XM_015277858.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-2(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277858.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG AAGTCCATAA AAAGATGATG CTCCACCTGT TGACTTTTGG GGGGGGAACT
    TCCCAGCCTC CTACATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133344.1
    CDS228..4448
    Translation

    Target ORF information:

    RefSeq Version XM_015277858.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 2 chain (COL4A2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277858.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACCTTT CACCGCTTCC CGGCGCGCGT GTTTCTGTTT GTCAGGACGT GGGGCTTCTG 
    CTGGTGCTGC TGGAGGTCGT GCTGTCGGCC GGCAGAGTCG ATGCCGGTGG AAAGAGTTAC
    ACAGGACCAT GTGGAGGAAG AGACTGCAGT GGAGGATGCC AATGTTTCCC TGAAAAAGGA
    GCCCGTGGCC AGCCAGGAAT CCTTGGATCG CAGGGGTTCC CTGGACCACC AGGATTGATG
    GGGATCCCAG GACTGCAAGG CCCTAAAGGT CACAAGGGTG AAAGAGGACA TCCAGGAATA
    AGTGGACCCA AAGGAGAAAC GGGGCAAAGA GGAGTCACTG GTTTCCCAGG GGCAGATGGC
    GTTCCTGGTC ATCCAGGGCA GCCAGGATCA AGGGGGAAGC CAGGTCATGA TGGCTGTAAT
    GGGACAGTAG GTGACCCAGG TGACCCAGGA ACACCAGGAC ACTCTGGTTT CCCTGGTACC
    ATTGGAGTTC AAGGACCAAA GGGCCAGAAG GGGGAGCCCT ATGTGCTACC GCCAGACATC
    GCCAGCCGAC ATCGGGGGGA TCCTGGGGAC CCAGGATTCA CTGGTTTTCC GGGAGCTCCG
    GGTACATTGG GTATTCAAGG ACCTATTGGT CCAAGAGGAG TGCCAGGAAG ACCTGGACCA
    CCGGGGTCCC CAGGACCGCA AGGACCACAA GGCAACAGAG GTCTTGGCTT CTATGGCGAA
    AAAGGTGAAC AGGGCTCCCC AGGTCCTCCA GGTCCACCTG GACTTCCCAC AAGAGAACTG
    ATTGGTGTCC CCACTGACAA ACACAAAGGT GAAAGAGGAG AGCCTGGACA AAAAGGGGAA
    GCTGGATTTC CAGGTGTACT TCTCTTCGCT CCTGAAAAAG GTGAAGAAGG GGTAATGGGC
    TTCCCAGGAC AAAGGGGACT GCCTGGTAAC GATGGTTTTC CAGGACTCAG TGGAGAAAGA
    GGTTTTCCAG GATTTGATGG TCAACCAGGT CAATATGGTC CTAGAGGAGG CAAGGGGGAG
    CAGGGTGAGA TGGGTCCTCC AGGACCACCT GCCTATGTTC CTTACCGCAT TCCAAGAAAA
    GGTGTAAGAG GTGATCCAGG CTCTCCAGGT GCTTCTGGAC TACGGGGAGA ACAAGGAGAG
    CAGGGTGATC GTGGGCTGCC TGGTATTCCA GGATTCTCTG ATGGAGATGC AGATAAGCCA
    GGCTTACCTG GAGAAATAGG ACCAAAAGGT GAAAAAGGTG AAGAAGGATC TCCTGCTTAT
    CAAGCAGGCC CGCCTGGGTT TCCAGGTAAA CACGGTGAAC CAGGAATCCG TGGTCCACCT
    GGTCCCCCTG GAACTCCAGG TTCTCTCTTC GGATTAAAAG GACAGGAAGG GACACCAGGT
    AGACCTGGTG TACAAGGTTT TCCAGGGCCA AGAGGACAAA GAGGACCTAA AGGTGAAGAA
    GGTGACTGCA GTAAATGCCT TCTGAGTGAT GAACTCAGAA GGGGCTCTAC TGGCCCCCGT
    GGTCCTCCTG GTTTTCCAGG AACCCCTGGC CAGCCTGGGA GAAAAGGAGA ACCTGGTGAT
    CAAGGTCCAC ATGGTATTCC TGGCTATGCT GGAGCAAAAG GACAATCGGG CCAAGAAGGA
    TTACCTGGAC CAAAAGGAGA AAAGGGAGAT TCTATATACA TAACTACTAA AGGCACCAAA
    GGAATCCGAG GAGATCCTGG ACTTCCTGGT ATCAGAGGGG AAGATGGTTT CCCAGGCAGA
    GATGGATTAG ATGGTTTACC AGGACTGCCT GGCCTTCCGG GTGATGGTAT CAGAGGTCTC
    CCCGGGGATC CTGGTTATCC AGGAGAATTA GGCCCAAAGG GCTTTCCAGG AGAAATAGGC
    CTACCAGGTG AAGGATATCC TGGCCCAAAA GGATACCGTG GTCTTCCAGG TGACCGTGGA
    ACAGATGGAC ACCCTGGGCC TCCAGGTCTG CCTGGTCCAC CAGGAGAGCC TGGCCAATTA
    GACTGCGGGC AGGTGATTGA AGATTTTTCT AGAGGAGAAG CAACTGATCC AATATGGTCA
    GGTGGAGGCT GCGTGAGGCC ACCAAAGGGA TCTCAAGGCA ACCCAGGGCT GCCAGGAGCC
    ACAGGTACTA AAGGTGCAAG AGGATTCCCG GGTGATCCAG GCCCAGTGGG ATTCCCAGGG
    CTAAATGGTA CACGAGGTGA TCCAGGTCGG GAAGGATATC CAGGTCCTCC AGGCTTTATT
    GGGCCCCGGG GAGACAGAGG CCCAAATGGC CTACCTGGAC TACAGGGACA TCCAGGTCTT
    ATGGGAAAGT CTGGTGCTCC AGGACTTGCT GGACAGAAGG GTGCTCCTGG TGACGTTCTG
    GGAGCAGCAG CAGGTCCTCG AGGGGATGAT GGGCTGCCTG GCTTCCCTGG CTTGAAAGGT
    GCACCAGGAG ATCAAGGAAT ACCAGGAATT AGAGGAGCGG ACGGTAACCC AGGATTGCCA
    GGCCCCAAAG GTGACCCAGG GCTGCAAGGT CTCCCTGGTT TGATGGGTTT GCCAGGAACA
    CCAGGAACTC ATGGATTCCC AGGGCCACCA GGAAACCGCG GCCCTGACGG TGGACCCGGC
    TCTCAAGGAC CCCTTGGTCC ACCAGGTGCA AGGGGAGAAG ATGGCGAGCA AGGCTTTCCT
    GGTCCCGTGG GCATGAAAGG TCTGTCTGGT GACAAAGGTG AAACAGGTTT CCCAGGATTA
    CAAGGTATCC CTGGTGTGAC CGGTCCCCCT GGCATTAGTG GGATGGATGG CTTCCCAGGA
    GACAAAGGCT CAAGAGGTTC ACCTGGCATT GATGGTTTCA AAGGCATGCC AGGCCTGAAA
    GGAAGACCAG GAATCAAAGG AATCAAAGGA GAATTTGGCT TACTTGGGAC TCGGGGTGAT
    AAAGGCGCAC AGGGTGCACG AGGCTTTAAA GGTGACCGTG GAGAGCAAGG TCCCCCAGGA
    GAGCCCCCGA AGCTGAAGCC TAGCATGATG ATGGAAGTTA AAGGTGAAAA AGGAGATGCT
    GGAGAAACTG GCACCAAGGG ATTCTTTGGC ATAAAAGGTA GCAAGGGAAT GCCAGGCCTC
    CCCGGTAAGA CAGGAATTCC TGGGTCCCCA GGGCACCCCA GTTATGTGCC TGGTGTGAAG
    GGGGACATCG GTGCAAAAGG GCTGACGGGT CTGAAAGGGT ATCCTGGTCC GACTGGCTCT
    CCAGGCATCA GAGGCTTCCC TGGCAGCACA GGAGGCAGGG GAGATAAGGG TGCCCCAGGA
    ATTTCGGGCC ATTTTGGTAC TCCTGGCTCC CATGGTGAAA TTGGTGAGCC GGGCGATACT
    ATAAACTTAC CAGGAATGCC AGGCCTCAAA GGGGAAGTTG GTGTGCCGGG TCTTACAGGC
    TTAAGAGGAG GCCCTGGACA AAAAGGTGAA GGAGGTGATC CTGGTTTACC TGGCATTGAA
    GGCCTCAAGG GCATACAGGG TGTACCTGGC TCCTTGGGAC AGAAAGGTTT GCCTGGACTG
    GTCGGCCCTC CAGGGCAGCA AGGAAGCCCC GGGACGCCTG GATTCCAAGG AGAAAAAGGA
    GCTCCCGGCT GGCCTGGCTT ACCAGGACAA GCAGGCTTGC CTGGCTTAAG AGGAATCAGT
    GGACTGCATG GTTTACCGGG CACCAAGGGC TTACCAGGAT CACCAGGTCC AGACGGCTAT
    GGCTCTGCGG GCTTCCCAGG CCCTGTGGGT GACAAAGGAG AAGCAGGAGA GCCCAGCAGA
    GTGGAGGGCA GCCAAGGCCC GCCGGGTCAG AAGGGAGACA GAGGTGTTCC AGGAGTCCAA
    GGACCCTTTG GCATTCCTGG GCAGGATGGA CTTCCAGGGC CTCCTGGGAT TTCCAATATA
    TCAGGATATC CAGGCGATAC AGGTTCCCCA GGTCTAGATG GAGTGCCAGG CTATCCAGGA
    CTACATGGGC AGCCTGGAAT ACCAGCTCCA CCGGGAAGCA AGGGAGAAAG TGGACGAGCA
    GGTGTGAGCG GACAAGCTGG CCCCAAAGGG ACAAGAGGTG ATCCTGGATT ACCAGGGAGA
    CCTGGGATTC CTGGTTATCC TGGACCAAAA GGTCGTAAGG GTGAACAAGG TGTCATTGGC
    TTTATTGGCA CAGTAGGATT TCCAGGTGAT TTGGGACCCA TCGGACCCAA GGGGGATAGA
    GGGCTTACTG AAGTCCATAA AAAGATGATG CTCCACCTGT TGACTTTTGG GGGGGGAACT
    TCCCAGCCTC CTACATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.